LOCUS       DAOQXQ010000001         8069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_9411_length_8069_cov_3.578612, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000001 DAOQXQ010000000
VERSION     DAOQXQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9411_length_8069_cov_3.578612"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1862
                     /locus_tag="PKH81_00005"
     CDS             69..1862
                     /locus_tag="PKH81_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ96458.1"
                     /translation="MILANLPSYTQDQSTPPSVVPMIQDIQAQADQAIRAENWDSALR
                     ALTQGTRLYPQEPQFFFKRGSIYLEQGLYEPALKDLLNSEQLGLTGKDILYSLANCFA
                     LLNQDQNALQYQIRYLELVPDDVFEWSNLGWLYYKTNRIDEGIDTLKAVINRFGPDGN
                     LCVTLGNLYTSAFDYQNAKKYYDLAIDFAQERSQSYLVSVYYYNRSVLEEVFYRWDQA
                     NADSQKSLEAAPRSSGYLMQGELSLRRLDLPGAYNYYARAYAHDGTPLAVLGMADTLL
                     QGGKAVEAHETLKVLDNNTDYSWISHYGTTVDHFLADIHRLYRDIYHHRKGQQKDKII
                     HNFSTFINKYSAIISYSLHAWYHDGKYRIHSKLVAQRYAEDSQSSQFKGGLSLYKNSY
                     SFLALDTWRNVSQRYLRTAKDIEVSHIPASLPSYFLEEGKLFNNKVLLEQSIQTLDPI
                     WERKFKAQALGEYLFRFSRQSTTHYHKHSYDLFSLQPSAFTFYGLQLPVMVSIINSNL
                     PTSRIEKKHIRTLASTLLSGPFLADPDSPLVLQITSFSDRIELALIDTTKKFTICTQV
                     FHNKQNSTKKELVEQCNLFIHKVFSSQTLVQ"
     gene            complement(1911..2996)
                     /locus_tag="PKH81_00010"
     CDS             complement(1911..2996)
                     /locus_tag="PKH81_00010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ96459.1"
                     /translation="MSKRTKVLFFTVVLLLVMGGATAAAILLGGKTTKASEGGIMDTD
                     KETAAITSDGLYAVIETPKGSMTLELFYEKTPLTVCNFVGLAEGTLDAAKGKPYYNGL
                     VFHRVIENFMIQGGDPTGTGSGGPGYRFPDEIDPSLKHDAPGILSMANAGPGTNGSQF
                     FITHLPTPWLDGKHTVFGKIVEGQTVVNAIAGGDKIISVKIIRKGDSARKFKATQEDF
                     NRLLADQGNRLQQVAERQKKEFFDLIAKKWPKAQQSSSGVFFQINAQGSGESAAAGQT
                     LTMKYKGYLLDGTVFDDSDMRKPLEFQVGRGKLIPGFDSQALEMKKGEKRTIVIPPEL
                     AYGANGVPGVIPGNSFIAFDLELLEIK"
     gene            complement(2993..>3771)
                     /locus_tag="PKH81_00015"
     CDS             complement(2993..>3771)
                     /locus_tag="PKH81_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004790383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ96460.1"
                     /translation="GVAGDQQAALFGHTCFSPGMVKNTYGTGCFTLLNTGSWPVWSKH
                     NLLTTVAWKIKGKITYALEGSVFIAGAVIQWLRDELGLITTAAESEEIAESVSDSAGA
                     FVVPAFVGLGTPYWDPLARGSIVGLTRGVNRAHIVRAAIESIAYQSEDLFASMRADYG
                     KPIEVIKADGGATANRFLMQAQADISGIPVILPKNTEITALGAAYLAGLRAGYWNNLG
                     EIEKIWQTSASFEPKNEARTRERRLELWHKAVSATREFKV"
     assembly_gap    3772..3781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3782..4500)
                     /locus_tag="PKH81_00020"
     CDS             complement(<3782..4500)
                     /locus_tag="PKH81_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ96461.1"
                     /translation="MSETTNGFILSFDQGTTSSRAIIFDHDGKMVSSAQKEFTQFYPE
                     PGWVEHDPEEIWQTQIDAAHEAIAKAKIQVDQIVSIGITNQRETTVMWDKDSGRPVYR
                     AIVWQCRRSTSICQELIDSGLEDEFRQRTGLRIDPYFSGTKISWLFREIPGLKALAEN
                     GEILFGTIDSWLLWKLTGNHYTDITNASRTLLYNIGEQHWDPMLLSILGVPAQALPVV
                     KESSDDFGYTKKEIFGREIPV"
     gene            complement(4484..5275)
                     /locus_tag="PKH81_00025"
     CDS             complement(4484..5275)
                     /locus_tag="PKH81_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96462.1"
                     /translation="MKLFFSAVLFCCSLGLLPANDIQERLEKLISAENTEALLRDGCV
                     QRTVYRDDRAKPELTPRLDDCRDAIEFWEGDVPAFLSETLYYYPKKATLEGSGKNDTG
                     LVSKIIRSLSRLEGIEYFSTSRKKMRTLYEKSFVVDNEKSRNRIDDSTSGPADGLSLF
                     AVQKDLTFGEYLYRYTYRQTEHTTGFHSQNEESLSYTFVRLIKPRKLRVSLIIHDMGD
                     AFLVYNLTRIDFLAIPGIEGKIQSSFSTRARAMYDWFIREYERNN"
     gene            complement(5276..5995)
                     /gene="jag"
                     /locus_tag="PKH81_00030"
     CDS             complement(5276..5995)
                     /gene="jag"
                     /locus_tag="PKH81_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNQ96463.1"
                     /translation="MTYEFEGKTEKEAIEMAASELGLEKDQFDVEILEAQKNSIFKKG
                     FVKIRVHTDETVKRAIPEKKRSIDSEYDGSLPKPTGAFQDPLPKNEFEKAMIDFIASV
                     VRKMGYDVTVEVMSREEFKIGLRLNSRSSSILIGRKGKNLDALQLLANVYAGKIGRED
                     TRVILDSENYRIRREESLVRLAYTTADRVRTTRGSILLEPMNPFERRLVHTTLNDIGD
                     VDTKSEGEGLYKQVRVFYKGR"
     gene            complement(6021..7784)
                     /gene="yidC"
                     /locus_tag="PKH81_00035"
     CDS             complement(6021..7784)
                     /gene="yidC"
                     /locus_tag="PKH81_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNQ96464.1"
                     /translation="MDRNTVTAIVLSTVVIVAGFTIQSKFFPNKTAIPVQPRQEVPEL
                     TVIDSPSPATTEDSSDKSQQEPAFLEKDSGQEVQSEQRFFIETDLVRVEFTNKGGDIV
                     SYKLKQHKDRDELVEMASSVTDSNRAFSILLGSSEGQILDQYFNVKRINDNEIGFYRS
                     FTVQNADGSKGSFTLAKQYSFIPGQYLFELKVTIDGDAALDGLRFGDAAYTIKTSPQI
                     GPRWHVKEDKYEYRKFFYLLNGKKKTVTVNAGQSKSNNENFSWTAVAGKYFTLIAIPE
                     APLAGVRYSAVTIDPSVPVSQMYLTRPAISGNRNTDLWRFYIGPRTDKNLAMYNISTN
                     NPFGIGDAKLNEMVESSGILAPLEILLKLIMELFYSFIPNWGISIILMTILMRIVIFP
                     LTKKSSEATLKMQEHAPKMQEIQAKYKGNPQKLNEEMAKFYQSSGYNPLSGCLPLLIQ
                     FPLIFAMYNLFNNYFEFRGAMFIPGWIPDLSQGDSVLAFPFTVPLVNWTELRILPIIY
                     VVSQLLFGKVTQTPTSAQQNSSMKFMMYGMPLMFFFLFYNAPAGLLIYWTFSNILTLG
                     QQIVINKMMHAKKAKGLELVK"
     gene            complement(7815..8054)
                     /gene="yidD"
                     /locus_tag="PKH81_00040"
     CDS             complement(7815..8054)
                     /gene="yidD"
                     /locus_tag="PKH81_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ96465.1"
                     /translation="MCGAIRLYQRLISPLFPPSCRFFPTCSAYAYEAISRFGPCKGSW
                     MALRRIMRCHPLHEGGYDPVPKAGRSDESPDMEST"
BASE COUNT         2452 a         1791 c         1397 g         2419 t
ORIGIN      
        1 cggggtgtag gtatgtgatt tgttcatgac catgctttcg cttgataaaa ctatttctct
       61 gtatattctt gattcttgca aacctaccct catacactca agatcaatca acaccaccat
      121 ctgttgttcc catgattcag gatattcaag cacaagcaga tcaggctata agagcagaaa
      181 attgggattc tgccttacgg gctctaaccc aaggaacgcg cttatatcca caagaacctc
      241 aattcttttt taaaagaggt tcaatctacc ttgaacaagg actctatgaa ccggcgctca
      301 aggatctttt gaattcagaa caacttggtc tcactggaaa ggatattttg tattctctcg
      361 caaattgctt cgctcttcta aatcaggacc agaatgcact tcaatatcaa attcgatatc
      421 tggaacttgt tccagatgat gtgtttgaat ggtcaaatct tggatggctt tattacaaga
      481 caaaccgcat agacgagggc atagacaccc ttaaagcagt aattaaccga tttggcccag
      541 atggtaatct ctgtgttacc ttgggaaact tgtatacatc ggcttttgat tatcaaaatg
      601 ctaaaaaata ctatgattta gcaattgatt ttgcccaaga aagatcacag tcctatctgg
      661 tatccgtata ttattacaat aggtctgttt tagaagaagt attttaccga tgggatcagg
      721 ctaacgctga ctcgcaaaaa tcccttgaag cggcaccacg atcatctgga tacttaatgc
      781 aaggagaatt aagcttgcgc aggcttgacc tacctggtgc atataattat tacgctcgag
      841 cgtatgcaca tgacggcacc cctcttgcgg ttttgggtat ggcggacact cttttacaag
      901 gaggaaaagc ggtagaagct catgaaacgc ttaaggtact tgataataac actgattatt
      961 catggatttc ccattatgga actacggtag atcattttct tgccgatatc caccgtttat
     1021 atagagatat ttaccaccat cgtaaaggtc agcaaaaaga taaaattatc cacaactttt
     1081 ccacattcat taataaatac tccgcaatta tttcgtattc cttacacgca tggtatcatg
     1141 atggaaaata cagaatacac agtaaattgg ttgcacagcg ctacgcagag gatagtcaat
     1201 catcccaatt taaaggaggt ctatcactct ataaaaattc ctacagtttt cttgcattgg
     1261 atacatggcg caatgtgtct caaagatacc tccgaacagc taaagatata gaggtctcgc
     1321 atattcctgc ttcattacca tcatatttct tggaggaagg aaaacttttt aataataaag
     1381 ttcttttgga acaatcaata caaactcttg atccaatttg ggagcgaaaa ttcaaagccc
     1441 aagcccttgg cgaatatctt tttcgttttt caagacaatc tacaacacat taccataagc
     1501 atagttatga tcttttttct ctccagccat ctgcctttac gttttatggc ttgcaattac
     1561 ctgttatggt ttcaatcatc aatagtaatc ttcctacatc aagaatcgaa aaaaaacaca
     1621 ttcgtacact ggctagcaca ctattatctg gtccttttct ggctgatcca gattcacccc
     1681 tggtcctaca aataacaagt ttttctgata gaatcgagtt agcacttatt gatacaacaa
     1741 aaaagttcac aatttgtaca caagttttcc acaacaagca aaattccaca aaaaaagaat
     1801 tagtcgaaca gtgcaatctc tttatccaca aagtatttag ttcacaaaca cttgtacaat
     1861 aaaaaacagg gttgcctttc ggcaaccctg tacttcatat tagttttaac ttactttatt
     1921 tcaagaagct ctagatcaaa ggcaataaaa gaattaccgg ggataactcc gggaacgcca
     1981 ttcgctccat aagctagttc tggtggaatt acgattgtcc gtttttctcc ctttttcatc
     2041 tcaagtgctt gagaatcaaa accgggaatc aattttcccc taccaacctg aaattcaagg
     2101 ggtttacgca tatcggaatc atcaaacact gttccatcca acaagtaacc cttatatttc
     2161 attgtcagcg tttgacccgc cgcagcgctt tctcctgatc cttgtgcatt aatttgaaaa
     2221 aatacgcccg aagaagactg ttgtgccttt ggccattttt tggctataag atcaaaaaat
     2281 tctttttttt gcctttcagc aacctgttgt aaacggttac cctgatcagc caagagacga
     2341 ttaaaatctt cctgtgttgc cttaaattta cgagctgaat cgccctttct aataattttt
     2401 acagaaataa tcttatctcc acctgcaatt gcatttacaa cagtttgtcc ttcaacaatc
     2461 ttgccaaata cagtgtgttt tccatctaac caaggagtag gtaaatgagt aataaaaaat
     2521 tgacttccat tagtaccagg gccagcatta gccattgaaa gaataccagg ggcatcatgc
     2581 tttagcgaag gatctatctc atcgggaaaa cgatatccag ggccaccgct acctgttccg
     2641 gttggatctc ctccctgaat cataaaattc tcgattactc gatggaaaac tagtccatta
     2701 taatagggtt tacccttggc tgcatccaga gtcccttctg ccaatcccac aaaattacaa
     2761 acagtcaggg gtgttttttc ataaaacaat tccagcgtca ttgagccctt aggcgtctca
     2821 ataactgcat agagcccatc actggtaatc gcggcggttt ctttatcagt atccataatt
     2881 cctccctcac ttgctttagt agttttacca ccaagcaaaa ttgctgcggc tgttgcacca
     2941 cccattacta gtaataacac aacagtaaaa aaaagcacct tagttcgttt actcatacct
     3001 taaactccct tgttgcagac accgctttat gccaaagctc aagccgtctt tctcgagttc
     3061 tagcttcatt ttttggctca aaactagccg atgtttgcca tattttttca atttctccca
     3121 aattattcca gtaccctgct cgcaggcctg ccaagtaagc cgcccccaat gcagtaattt
     3181 cggtattttt tggaaggatt accgggatac cagaaatatc tgcctgcgct tgcatcaaaa
     3241 atctgttcgc tgttgcacca ccatcagcct taatcacttc tataggcttt ccgtaatcag
     3301 ccctcataga agcaaataaa tcctcgcttt ggtaggcaat actctcaatt gctgcacgta
     3361 caatgtgtgc tctattaact cctctggtta gtccaactat tgagccccta gctaagggat
     3421 cccaatacgg tgttccaaga ccaacaaagg ctggaaccac aaaagctccc gctgaatctg
     3481 atacagactc agctatttct tcagactctg cagctgtagt tatcaagcca agttcatccc
     3541 gtaaccactg gattacagca ccagcgataa atacagaacc ctcaagggca taggttattt
     3601 taccctttat tttccatgcc acggttgtca ataaattatg tttagaccaa acgggccaag
     3661 atcctgtatt caataaggta aaacagccag taccatacgt attttttacc atcccaggcg
     3721 aaaaacacgt gtgtccaaat agtgctgctt gttggtctcc tgccactccc tnnnnnnnnn
     3781 ntgaacagga atctctctac caaaaatttc ctttttagta taaccaaaat cgtcagatga
     3841 ttccttgact accggaaggg cttgcgcagg aacacctaaa atcgaaagaa gcataggatc
     3901 ccagtgttgc tcaccaatgt tatatagcag ggttctagat gcgttagtta tatctgtata
     3961 atgatttccg gttaacttcc ataaaagcca tgaatcaatc gtgccaaata aaatttcgcc
     4021 attctcagca agggccttaa ggccgggaat ttctcgaaac aaccagctaa tcttggtacc
     4081 tgaaaaataa ggatcaattc gtaatcctgt tcgttgcctg aattcatctt ctaatccaga
     4141 atcaattaat tcctggcata tactagtact tcttctacat tgccagacaa tcgctctata
     4201 cactggtcta cctgaatctt tatcccacat gaccgttgtt tcacgttgat tggtaattcc
     4261 tatcgaaact atttgatcta cttgaatttt tgccttggct atagcctcat gggctgcatc
     4321 tatttgtgtt tgccaaattt cttcgggatc atgctctacc cagcctggtt cgggataaaa
     4381 ctgtgtaaat tctttttgtg cacttgaaac catctttccg tcgtgatcaa atataattgc
     4441 acgggatgag gtagttcctt ggtcaaaaga aagaatgaat ccgttagttg tttcgctcat
     4501 attctcttat gaaccaatca tacatagctc gagcacgagt agaaaacgat gattgaatct
     4561 ttccttcaat acccggaata gctaaaaaat caattctggt aagattatac accaaaaaag
     4621 catctcccat atcatggata attaaggata ctcgcaactt tcttggtttt atcaaacgaa
     4681 caaaggtgta agatagagat tcctcatttt gcgaatggaa tcctgttgta tgttcagttt
     4741 gacgatacgt ataacgataa agatactcac caaaggttag atctttttgt actgcaaaca
     4801 aagacaatcc atctgctggg ccagaagtcg aatcgtcgat tctgtttctg cttttttcgt
     4861 tatcaactac aaacgatttc tcgtagagcg tacgcatctt ttttctgcta gttgaaaaat
     4921 actcaatacc ttccaatcga gacaaggaac gaataatttt tgaaaccagt cctgtatcat
     4981 ttttaccaga accttctaaa gtagctttct tggggtaata atataacgtt tcactcagaa
     5041 aggctggaac gtctccttcc caaaattcaa ttgcatcgcg acaatcatct aacctcggtg
     5101 ttaactctgg ttttgcccga tcatcgcgat atactgtcct ctgcacacat ccatcacgaa
     5161 gtaaggcttc cgtattttca gcggatatca acttttccag ccgttcctgt atatcgtttg
     5221 ccggaagcaa acccagcgag caacaaaaca gtacagctga aaaaaaaagt ttcatttatc
     5281 gacccttata aaaaacacga acttgcttat acaatccttc gccttcgctt ttggtgtcta
     5341 catcaccaat atcattgagt gttgtatgta caagccttct ttcaaaggga ttcataggct
     5401 caagcaaaat cgatccgcgc gtagttcgaa cacgatcagc tgtagtataa gcaagtctga
     5461 ctaaggattc ttctcgtcga atacgataat tctcggaatc aagaattacg cgggtgtctt
     5521 ctcggccaat tttacctgca taaacatttg ccagtaactg caaagcatca agattttttc
     5581 cttttcgtcc tatcaaaata gaagaactac gagaattaag tcgcaaacct atcttaaatt
     5641 cttcacggga cataacctca accgtaacat catatcccat ttttcgcact acagatgcaa
     5701 taaaatcaat cattgccttt tcaaattcat tcttaggaag cgggtcctga aaagcacctg
     5761 tcggttttgg aagcgaacca tcatattcac tatcaataga tcttttcttt tccggtattg
     5821 cgcgttttac tgtttcatca gtatgaacac gaattttaac aaaccctttt ttaaagatac
     5881 tgtttttctg cgcttccagt atttccacat caaattgatc tttttctaga cctaattccg
     5941 aagcagccat ctcaatggct tctttttctg tttttccttc aaactcatat gtcatatagt
     6001 gcactcctga ctataatgtg ttatttaacc aattcaaggc ctttggcctt ttttgcatgc
     6061 atcatcttat tgattacaat ttgctgaccc agggtcagta tattggagaa cgtccaatat
     6121 attaacaaac ctgccggtgc attgtaaaac aagaagaaaa acattagcgg cataccatac
     6181 atcataaact tcatagaact attttgttgc gcacttgttg gtgtctgcgt tactttacca
     6241 aacaacagtt gtgaaacaac ataaataatc ggtaagatac gcaattcagt ccaatttacc
     6301 agagggacag taaagggaaa ggctaataca ctgtctcctt gggaaagatc cggaatccat
     6361 cctggtataa acatagctcc gcgaaattca aaataattat taaacaaatt atacatcgcg
     6421 aaaataagcg ggaattgtat caataaaggc aagcacccag aaagaggatt ataaccagaa
     6481 gattgataga actttgccat ttcctcattc aatttttgag gattaccctt atacttggct
     6541 tgaatctctt gcatcttggg cgcatgttct tgcatcttaa gggtagcttc agagcttttc
     6601 ttggttagcg ggaaaataac tatacgcatc aatatagtca tcaaaattat tgaaataccc
     6661 cagttaggta taaaagaata aaaaagttcc attatcaatt taagtaaaat ttccaatggt
     6721 gctaaaatac cgctactttc taccatttca ttcaatttgg catcaccgat cccaaaagga
     6781 ttgtttgtcg aaatattata catagccaga tttttatcag tccttggacc aatataaaaa
     6841 cgccataaat cagtattccg attaccagat atcgcggggc gagtaagata catttgagaa
     6901 acaggcactg atggatcaat cgtaacagca gagtatcgga caccagcaag aggggcttct
     6961 ggtattgcaa tcaatgtaaa atatttccca gcaactgctg tccaggaaaa attttcgtta
     7021 ttagacttac tttggcctgc gtttacagta acagttttct tcttgccatt aagtaaataa
     7081 aagaatttac gatactcgta tttatcttct tttacatgcc acctaggtcc aatttgagga
     7141 gaagttttta tagtatatgc tgcgtcacca aaacgtaacc catcaagggc cgcatctcca
     7201 tcaatagtca cttttaactc aaaaagatac tgtcctggaa taaaggaata ttgcttggct
     7261 aaagtaaaag agccttttga accatcagca ttttgaacgg taaaagatcg ataaaaacca
     7321 atctcattat catttatccg tttcacatta aaatactgat ccagtatctg accctctgaa
     7381 gaacctaata aaatggaaaa agctcgattg gaatcagtaa ccgatgatgc catttctact
     7441 aattcatcgc gatctttgtg ctgtttcaat ttataagaaa caatatcgcc acctttattg
     7501 gtaaactcaa cacgtacaag atctgtttca ataaaaaacc gttgctcaga ttgaacctct
     7561 tgtccagaat ccttctcaag aaaggcaggc tcttgttgtg atttatcaga agaatcctct
     7621 gttgttgccg gggatggcga gtctataaca gtaagctctg gaacttcttg gcgaggttga
     7681 actggaatcg ctgttttgtt tggaaaaaat ttagactgaa tagtaaagcc ggccacaatt
     7741 acgaccgtag aaagaacgat agccgttaca gtatttctat ccatattaca cttgctcctt
     7801 ttctggaatc atacttatgt tgattccata tccggacttt cgtccgatcg tccagccttg
     7861 gggactgggt cataaccgcc ttcgtggagg ggatgacagc gcataatgcg acgtaaagcc
     7921 atccaagacc ctttacatgg tccaaaacgg cttattgcct cataagcata cgcagagcat
     7981 gtcggaaaaa aacggcaaga aggaggaaat aaaggggaga ttaatcgctg atacaagcgt
     8041 attgccccac acataagggc aacaagaac
//
LOCUS       DAOQXQ010000002         8055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_9432_length_8055_cov_3.712500, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000002 DAOQXQ010000000
VERSION     DAOQXQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_8055_cov_3.712500"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="hflX"
                     /locus_tag="PKH81_00045"
     CDS             complement(<1..946)
                     /gene="hflX"
                     /locus_tag="PKH81_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ96466.1"
                     /translation="MIEIEREELKPVRAVLVGLAENRTATDPNPVHELESLASTAGLE
                     CAAQIVLTKPADSPRYGVGSGKALEIKTVALECEAEYIVFDFEITPSQQRNWESLTDL
                     TCIDRQELIIRIFAARAMTREAALQVELARLEYALPRLSHTYSALSRQRGGRYGTKGS
                     GESQLELDRRSIVDRIARLKVELKQVRQNRSTMRKRREKKPVPSCAIVGYTNVGKSSL
                     LNALTGARVLSEDKLFATLDPTTRRFILPTGRTVLLTDTVGFIRNLPHGLVDAFHATL
                     EEAALATAVICVLDASDPALDVQYETTLRVLREVGAADR"
     gene            1088..3196
                     /locus_tag="PKH81_00050"
     CDS             1088..3196
                     /locus_tag="PKH81_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96467.1"
                     /translation="MRTGAKIAVSTLISLFLFTGLTVASYAGLFSILENRFYAPSIIK
                     NYSDQGERAARILDSWHSDNVAAFSTFTANEAVTRSLLPNQSSQDIFDRTNLAGTLMA
                     GTRGLEGIRIIDSGESIRENDTGNRRIHFSTFTQDILRKEDFLISWKQYGRDDADIPF
                     ARIARSDGDSARLILDTERDRYLYTFPFYDSYGTWRGTAVFYVSARAAVQKLIGEGLL
                     RLSDHPVLIATEDALVYGAVTGMPSAGRDFLTNVIIDRWTKNDLAADRIVGTGESGWV
                     LVSKKAGEWGIYGQLVDEQLFSFPKSVRTLFLAVSFLTMFLVIFLLFNLKQDEMVIIR
                     SRIRRFQVNLMEELMETDEEQQWEELRKNLTGKKREFNDRIKRGFGRRVKRKHSEAID
                     QLLDKSWEEILTAIGRKEHTQKIGTEEIRLMLEQVLQNNAISLNLTNVTTGMQSQRPV
                     APDTTPPDAMEDLEEVEELDEVESVEELDEVESVEELDEVESVEELDEVESVEELDEV
                     ESVEELDTDPAYAPAEPDEVEYLDGEWEVELLDVATDEELAEFIEESVPDDILVYDFG
                     EKPGFDEPVRPIPAEQESYSFAEFEISGLDFTSLDELLSDTTEAEEDAENAIWIGDIL
                     PGQTPSRSILLSERHPVEFLEVIGEDEPSPLLDVEETQNDGGTIINRDGIYVISAATK
                     LEDPEGINSDFRELVNSVLH"
     gene            complement(3197..3601)
                     /locus_tag="PKH81_00055"
     CDS             complement(3197..3601)
                     /locus_tag="PKH81_00055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNQ96468.1"
                     /translation="MIHVAAAVIENREGRILIARRKAEISLGGYWEFPGGKMEAGESP
                     AVCAARELHEEMDVRIETGDVVAETTHDYGDKVVHLVAVRAILLDGHMRLHDHDEIRW
                     VAVSEMSDFLFAPADEAIVSALVAAHRHVKIH"
     gene            3667..4065
                     /locus_tag="PKH81_00060"
     CDS             3667..4065
                     /locus_tag="PKH81_00060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ96469.1"
                     /translation="MSFSTKLTVRSYELDAYNHVNNSIYLQYLEYGRMEFLKSIGFNY
                     PKLFESGYFLYVTHIDIRYRASARLFDELTIEVVPIKLGKLSGTFHQKITNQAGDLCA
                     EADVSWGCVDKTGKPSKIPDEFVVPALKPE"
     gene            4091..4468
                     /locus_tag="PKH81_00065"
     CDS             4091..4468
                     /locus_tag="PKH81_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNQ96470.1"
                     /translation="MHFTEVVMPLLEVISDIISIVSVGVVIYGTVIAIMKFARNEIGR
                     FGGLYTIHQIRVLRADLGTYLLLGLEFLIASDILKTIVEPGMKELVILGGIVFLRTVL
                     SFFLDREIRLIEQEARDFMDIKR"
     gene            complement(4449..5255)
                     /locus_tag="PKH81_00070"
     CDS             complement(4449..5255)
                     /locus_tag="PKH81_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNQ96471.1"
                     /translation="MNTSSFHTHTRLCKHASGEPAEYVLQATKDGCSALGFSDHCPYP
                     DDATWPGSRMSVRDVSLYREQVLSLRQDAPFPIYFGFECEWFPLYESWYRDFIRGECG
                     AEYVVYGSHWVRDAGEFWYIPEAAEKRLLGSYLDLTLQALETGLYDLFAHPDIFLAGY
                     TRMDSDIQNACQQIIASAIHHDIPMEINGLGLKKPFVYGDSGLRPPYPVREFWEMAAD
                     MGAKIVCSSDAHRSEDVIAGARFAKAQADEWGIQTMDPYEVLGLPSVLYP"
     gene            complement(5300..6847)
                     /locus_tag="PKH81_00075"
     CDS             complement(5300..6847)
                     /locus_tag="PKH81_00075"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HNQ96472.1"
                     /translation="MFHSRTMAEESGNIFERLIKVFFSSTDPEAEKRRQLKAIAKDLS
                     KNKYKWYRPGSEEALPAMAKFFYELYKVVGAAQLILANANSSMVLKNVLVEMSFSDNQ
                     AKLKERFSEDSIKERAKTVAPKELALQAKNELAAFMAEFDAEKIRKIDLLYAQLELLA
                     GFVCFDYFFLLKKFDSGIPERSFNYTPKFESIRGEYVLEDLKDFALVAYALPLDADWP
                     RIFSIVKAYREVDPVAIGAWNKLTNSLAEIRRSAVLEQIIRHLDKNPAYAVQVEPISP
                     RIVDTYLMKLKTQTEMVIQQIQQEKKTSKATELLKQIFGTDSIVRLKNYAERSNAAFE
                     KKMLGGYLYVEELNYLKAFLIDYFKRDIRALTDLFLVRGKWAAPAMSQGYSGSFHELL
                     ELSDKITAFDDALAEDDEIGSKLRAMLNRAERDKEVVKQLRTQLKDVNDKALAFLTEG
                     TQHFIVIARSLKTILEDHGKSPHALITNWKELELNADQPIKEWIVSVYKQIYAFVMLM
                     QLFLKEE"
     gene            6981..7604
                     /locus_tag="PKH81_00080"
     CDS             6981..7604
                     /locus_tag="PKH81_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HNQ96473.1"
                     /translation="MALSKPSAERLSRLAHLLEQRHDRHTPVSSTELERLTGWPSHTI
                     RKDISALSLPETVSTTAGYDSHLLATAIRQSLGFGEETQNCCIVGLGRLGSAFLEYTG
                     FTDSPFTLCAGFDSNVNRIEILKASVPLFPTFKMKEIIPRLAIRYAILCVPPEQAQNT
                     AIRLADCGVAGIVNFTPAIVRPIEGVEIEQVSIVDVLGGLAARLAAT"
     gene            7627..>8055
                     /locus_tag="PKH81_00085"
     CDS             7627..>8055
                     /locus_tag="PKH81_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ96474.1"
                     /translation="MKRVYNFSAGPSMLPQEVLEKAAAEILDFNGTGQSVMEMSHRSK
                     AYKPIIDKTEALLRELMNIPANYKVLFLQGGASLQFAMIPLNLKKKGKAAYIDTGVWS
                     GKALKEAKKWIEVDVVASSKDKTYNYIPKVDKVTGDYDYVH"
BASE COUNT         2220 a         1972 c         1845 g         2018 t
ORIGIN      
        1 gacgatcggc ggctccaact tcccgaagga ctcgcaacgt cgtttcatat tgtacatcaa
       61 gggcaggatc gctggcatca agcacacaga ttacagccgt tgccagggcc gcttcttcca
      121 acgtcgcatg aaaggcatcg accaatccat gtggaagatt ccggataaaa ccgacagtat
      181 cggtaagcaa taccgtcctc ccggtgggta gtataaaacg tcgggtggta ggatcaaggg
      241 tagcaaaaag cttatcctcg gacaaaactc gcgctccggt aagggcgttg agcaagctcg
      301 attttcctac gttagtatag ccaacgatgg cgcaagaagg aaccggtttt ttttcccggc
      361 gcttgcgcat agtggagcga ttttgacgaa cctgtttgag ttctacctta aggcgagcga
      421 tacgatccac gattgaacgg cgatccaact caagctgtga ttcacctgag cctttagtgc
      481 cataacgtcc accacgctgg cgcgaaaggg ccgaataggt atgagaaaga cggggaagag
      541 catattcaag tcgagctaat tcgacctgaa gcgcagcttc tcgggtcatt gccctggcgg
      601 caaagatacg aataataagc tcttgtctgt caatacaggt taaatcagtc aaagattccc
      661 agtttcgttg ttgcgaaggg gtaatctcaa aatcgaacac aatgtattct gcctcgcatt
      721 caagggctac agtcttaatt tccagagcct ttccggaacc aacgccgtaa cggggagaat
      781 cggcaggctt ggttagcaca atctgtgcag cgcattccaa tccggcagtg cttgcaaggc
      841 tttccagctc atgtacagga ttagggtcag tcgcggttct attttcggca agtccaacca
      901 aaacagcccg aacgggcttg agttcctcgc gctcaatctc tatcattagg gtaactatac
      961 cctaccgtga cagaaaagaa caggcgccag gtcgccttga ttggtgaact catagatcct
     1021 ttctagctaa tgatttaccc gaaacggtcc gatatagtaa aagactcagt ggattttcgg
     1081 aggctatgtg cgcaccgggg caaaaattgc ggtaagtaca ctcatctcgc tcttcctctt
     1141 tacaggcctt acggtagctt cgtatgccgg gctgttttcc attttggaaa accgttttta
     1201 cgcgccttcc attattaaaa actactcaga tcagggagaa cgggcagcca gaatcctcga
     1261 ttcctggcat tcagataatg tagcagcctt ctctaccttt actgcgaacg aggccgtaac
     1321 aagaagcctt ttgcccaacc aaagctcaca ggatatcttt gaccgcacaa acctggccgg
     1381 aactcttatg gcgggaacta gaggacttga gggaattaga ataatcgatt ccggagaaag
     1441 catccgtgaa aacgatacag gaaacaggcg aattcatttt agcaccttta cccaggacat
     1501 ccttcgcaag gaagacttct taatttcctg gaaacaatat ggccgggacg acgctgacat
     1561 tccctttgcc cgaatcgcac ggagcgacgg tgatagtgcc cgcctgattc ttgacacaga
     1621 acgggaccga tatctgtaca ccttcccctt ttatgactcc tacggtacct ggcgtggaac
     1681 tgccgttttt tatgttagtg cccgagctgc tgtacaaaaa cttatcgggg agggactact
     1741 ccgcctgagc gaccaccccg tcctgatagc tacggaagac gctctcgtat atggagcggt
     1801 aaccggcatg ccttctgccg gacgggactt tcttacaaat gtcatcattg atcgatggac
     1861 aaagaatgat cttgctgcag accggattgt agggaccgga gagtcaggtt gggttctcgt
     1921 atcaaaaaaa gccggtgaat ggggaatata tggacaactt gttgacgagc aattgttctc
     1981 ctttcccaaa agcgttagaa cgctgtttct ggcagtcagt tttcttacca tgtttcttgt
     2041 catcttcctt ctgtttaacc ttaagcagga tgaaatggtc attatcagat caagaatcag
     2101 gcgattccag gttaacctta tggaagagct tatggaaact gatgaggagc aacagtggga
     2161 agaactgcgc aaaaacctga ccggaaaaaa acgcgaattc aatgaccgta tcaaacgcgg
     2221 ttttggcaga cgcgtcaagc gcaaacacag cgaagcgata gatcaactcc tggataaaag
     2281 ctgggaagaa attctaaccg ctattggacg caaggaacat acccaaaaaa tcggaaccga
     2341 agaaatcagg cttatgcttg aacaggttct tcaaaacaac gcgatatccc ttaaccttac
     2401 caacgttaca acgggcatgc aaagccaaag acccgtagcg cctgacacta caccgccaga
     2461 tgcaatggaa gaccttgaag aggtcgagga gctcgacgag gtagaatctg tcgaagagct
     2521 cgacgaggtt gagtctgtcg aagagctcga tgaagttgaa tctgtcgaag aactcgacga
     2581 ggtagagtct gtcgaggagc tcgacgaagt tgaatctgtc gaagagctcg acactgatcc
     2641 agcctatgct cccgccgaac cggatgaagt ggaatatctg gacggggaat gggaagtcga
     2701 gcttctggat gttgcaaccg acgaagagct cgccgagttt atcgaagaat ctgtcccgga
     2761 tgatatcctt gtctatgact ttggtgaaaa gcccggattt gatgagccag tcaggcctat
     2821 tcccgcagaa caggaatcgt attcctttgc cgagtttgaa attagcggac tcgactttac
     2881 ctctctggac gagctattat cagataccac tgaagcagaa gaagacgccg aaaacgccat
     2941 atggatcggc gacatccttc caggccaaac accatcccgt tcaatactcc tttcggaacg
     3001 tcacccagtt gaatttttgg aagttattgg agaggacgag ccatccccct tgcttgatgt
     3061 agaagaaaca cagaatgatg gtggaacgat tattaatcgg gatggcattt acgttatttc
     3121 agccgctacg aaacttgagg accccgaagg aattaattca gatttcaggg agttggtaaa
     3181 ctcggtttta cactagtcaa tggattttta catgccgatg cgcggcaaca agtgcggaca
     3241 ctatcgcttc atctgccgga gcaaataaaa aatcggacat ctcggatact gcaacccaac
     3301 gaatctcgtc atgatcatgc aatcgcatat gaccatccaa taagattgcc ctgaccgcca
     3361 ccaaatgaac aaccttgtcc ccgtaatcgt gtgtcgtctc ggcaaccaca tctccggttt
     3421 caatgcgaac atccatttct tcgtgtagct cgcgtgccgc gcatacggct ggactttccc
     3481 ctgcttccat tttacctccg gggaattccc aatacccacc aagagatatc tcagccttgc
     3541 gtcgtgctat caggatgcgt ccctcacggt tttctattac agcggctgcg acatgtatca
     3601 taccttaagt ataagagtca aaacttcaga ttgtcaaaac cgattacgga gtgtatcctg
     3661 taatgcatga gctttagcac aaaacttacc gttagaagtt atgaacttga cgcctacaac
     3721 catgtaaaca attcaattta tctacagtat ctggaatacg gacgaatgga atttcttaag
     3781 tcaattggct ttaactaccc aaaacttttt gaatccggat actttctcta tgttacgcac
     3841 attgatattc gctatagagc ctctgcccgt ctttttgatg agctcaccat cgaagttgtc
     3901 ccgataaaac tgggtaaact atcgggaacc ttccatcaaa aaataaccaa tcaagcaggg
     3961 gatctgtgtg cggaagcaga tgtatcctgg ggctgcgtag acaaaaccgg caagccttcc
     4021 aaaattccgg atgaattcgt ggtgcccgct cttaaacccg aataactacc aatggtcaaa
     4081 ggagtctgct atgcatttta ctgaagtagt aatgccctta cttgaagtta tttccgatat
     4141 catcagcatc gtgagcgttg gtgtcgtgat atacggaacc gttatcgcta tcatgaaatt
     4201 tgccagaaat gaaattggta gatttggcgg actctacacc attcatcaga ttcgtgtact
     4261 gcgggcagat ctgggaacct acctgcttct tggacttgaa tttcttattg cctcggacat
     4321 tctaaaaaca attgttgaac caggaatgaa ggagctcgtt atcttgggtg gaattgtatt
     4381 tctgcgaact gttctttcct ttttcctgga tcgagaaatt cgtctaattg aacaggaagc
     4441 cagagatttt atggatataa aacgctaggc aacccaagaa cctcgtacgg gtccatcgtt
     4501 tgaatacccc attcatcagc ctgggcttta gcaaaccgag ctccagcaat aacatcctcg
     4561 cttcgatgcg cgtcagagga acacacaatc ttggccccca tatcggctgc catttcccaa
     4621 aactcgcgga ctggataggg aggccgaagc cctgaatccc catacacaaa gggtttcttt
     4681 aatccaagtc cattaatttc catcgggatg tcatggtgta ttgcggatgc aataatctgc
     4741 tgacaggcat tttgaatatc gctgtccatt cgagtatatc ccgccaagaa tatatctgga
     4801 tgagcaaaca gatcgtaaag accggtttca agcgcctgta gggttagatc aagataagag
     4861 cccaagagcc gcttctctgc agcctccgga atataccaaa actcacctgc atcccgaacc
     4921 cagtgagatc catacaccac atattctgcc ccgcactcgc cccgaataaa atcgcgatac
     4981 caggattcat acagagggaa ccattcacat tcaaatccga aataaatagg gaatggagcg
     5041 tcttgccgca gggacaggac ctgttcacga tacagggata catcgcggac agacatccga
     5101 cttcctggcc aggtagcgtc atcgggatat ggacaatgat cggaaaaacc aagagcggag
     5161 caaccatcct tggttgcctg aaggacatac tctgccggtt cgccggaggc atgcttgcat
     5221 aaacgggtgt gggtatgaaa agagcttgtg ttcacgtatg gtactatagc gacccaagca
     5281 aaaagggaca aggctaccgt tattcctctt tcaggaacaa ctgcataagc atcacaaagg
     5341 cgtatatttg cttatacacc gaaacaatcc attcttttat gggctgatcg gcattaagct
     5401 caagttcctt ccagttggta atcaaggcat gcgggctttt accgtgatcc tccaaaattg
     5461 ttttaagact acgcgctatc acgataaagt gctgggttcc ctcggttaaa aaagcaagag
     5521 ccttatcgtt tacgtctttg agttgggttc gaagctgttt aaccacttcc ttgtcccgtt
     5581 cagcacggtt cagcatcgcc cgcaacttgg aaccaatctc gtcatcttcg gcaagggcat
     5641 catcaaaggc ggtaatcttg tccgaaagct caagtaattc atggaagctg ccagaatatc
     5701 cctgggacat tgcaggagcc gcccacttcc ctcgaactaa aaacaaatct gttaaggctc
     5761 taatatcgcg cttaaagtag tcgattaaaa aagccttaag gtaatttaac tcttccacat
     5821 ataaataccc gccaagcatc tttttctcaa aggcagcgtt ggatcgttct gcatagtttt
     5881 taagtcgtac tattgaatca gttccgaata tttgcttaag gagctctgta gccttactgg
     5941 tctttttttc ctgttgaatc tgctgtatca ccatctcggt ttgggtttta agcttcatca
     6001 agtaggtatc tacgattcgg ggggaaattg gctcaacctg taccgcatag gccgggttct
     6061 tatccaaatg ccgtatgatt tgttcaagta ccgcggatcg gcgaatttcg gccagtgagt
     6121 ttgtcagctt attccaggcg ccaatagcta ccggatctac ctcgcgatac gcctttacga
     6181 tcgaaaaaat acgcggccaa tctgcatcca ggggaagagc ataggcaaca agagcaaagt
     6241 ctttaaggtc ttccaacaca tactcgccac gaatggattc aaactttggc gtataattaa
     6301 atgatcgctc cggaataccg gaatcaaatt ttttaagtag aaagaaataa tcaaaacaga
     6361 caaaacccgc gagtaattca agctgtgcgt acaacaggtc tatctttcga atcttttcag
     6421 catcaaactc cgccataaag gctgccagtt cattctttgc ttggagcgca agctccttgg
     6481 gagcgaccgt ttttgcccgt tccttgatgg agtcttccga aaatcgctct ttaagcttgg
     6541 cttgattgtc cgaaaaggac atttcaacca ggacattctt aagcaccata gaagaatttg
     6601 cgttggcaag aatcaactga gcggctccaa ctaccttgta tagctcataa aaaaacttgg
     6661 ccatcgcggg gagggcttcc tccgagccgg gacgatacca tttatacttg tttttggata
     6721 aatctttggc aatcgctttt aattgccggc gtttttctgc ctcagggtcg gtagatgaaa
     6781 aaaagacctt gattaaacgc tcaaaaatat tcccgctctc ttctgccata gttctactat
     6841 ggaacataaa aaacctgctg tcaatcgaag gcgtgctttg aagattgttg tgaaatattt
     6901 cacaaaaaat agccttttaa cttgacctcc ttctactcgc ttgatatact aatagtgaaa
     6961 taattcacaa ggaggtgggt atggcccttt ctaaaccatc tgcagaacga ctatcgcgcc
     7021 ttgcccatct tctggaacaa aggcatgacc gacacacgcc tgtttcttct accgaactgg
     7081 aacgccttac cggttggcca agtcacacca ttcgcaagga tatctcggct ctgtccctcc
     7141 cggaaacggt gtctaccacc gcaggatatg attcgcacct actggcaacg gcaattcgcc
     7201 aaagcctcgg gtttggcgaa gaaactcaaa attgctgtat tgttggcctt ggccgccttg
     7261 ggtctgcgtt tttggaatat accgggttta ccgacagccc ctttaccctg tgcgcagggt
     7321 ttgattcaaa cgtaaatcgg atagagatac taaaggcttc agtacctctt ttccccacct
     7381 tcaagatgaa ggaaattatc ccccggctcg ccatacgata cgccatactg tgcgttccac
     7441 ccgagcaagc ccaaaacacg gcgattcgcc ttgccgactg cggcgtggca ggaatcgtta
     7501 attttacccc ggctatagtg cgccccatag agggcgttga aattgaacaa gtatccattg
     7561 tcgacgtgct tggcggcctt gccgcgcgac tcgccgcaac gtaaaaagac acgaaaggag
     7621 taacacatga aacgagtgta caatttctct gcaggacctt cgatgcttcc ccaggaagtc
     7681 ctggaaaaag cagcggcaga aattctggat ttcaacggaa ccggacagtc ggtaatggag
     7741 atgagccatc gatcaaaggc ctataagccc attatcgata aaactgaagc cctgcttcgg
     7801 gaactcatga acattcccgc aaattacaag gtactcttcc ttcagggtgg agcaagctta
     7861 cagtttgcaa tgattcccct taatcttaag aaaaagggca aggctgccta tatcgatacc
     7921 ggtgtatggt caggaaaagc gcttaaagag gcaaaaaaat ggatcgaggt tgacgtggtc
     7981 gcttcttcca aggacaagac ctataactac atccccaagg ttgacaaagt tactggcgac
     8041 tacgactacg tccac
//
LOCUS       DAOQXQ010000003         7897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_9645_length_7897_cov_3.263071, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000003 DAOQXQ010000000
VERSION     DAOQXQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9645_length_7897_cov_3.263071"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKH81_00090"
     CDS             complement(<1..1133)
                     /locus_tag="PKH81_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNQ96475.1"
                     /translation="MIGSILRKTLQGSPIARFISILVSFAVFFLLSSTVYLLSHRNPS
                     LISILPPIGEPGTVIVLQGKHFGQRANDGWVEIAGNRISGNAYISWNDSTIMLTLPET
                     VKDGLVYVSRGRFRSNPLMYANKKNIPITRRGTQTPDMPVIQAISQDRIEIGKVLTLR
                     GKNFGLTRGNSRVLFTWNADSQAILAVNQKKDSMVPCSEQDFDYEFWSDQEVRIRVPD
                     GASTGSLVLETERGISNSQEITIISKPGTKEYTSPRTYAISLQVDITDIQASAANILY
                     LRLPIPKTTTWQRDIELTASKPMPYMDNYRGTILHQLENIKSGQTERISHTFVLTGYG
                     VTTKIQSQLVKPFSDTRSPLYLVYTAADPLVPSSHQDIILKAA"
     gene            complement(1130..1837)
                     /locus_tag="PKH81_00095"
     CDS             complement(1130..1837)
                     /locus_tag="PKH81_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNQ96476.1"
                     /translation="MSTCPDADLYSAYVDGEVPSPWKEKLSQHLSNCPACRSKTDTYL
                     RLSQTMHHDKALLSPEQMDASLARLYDKMNERKARKSMGNAITTTRFFHASIQLPLPA
                     LAAALVLAVCIPVWVARGGSSNSPSDVLHASAATQAHTISPFNDEDLRVPVSSMSIYS
                     PDIPSIQLKNELMPDSGKKIFSMLNYARRFNSEADLFRDADIVIIRLPELTRFDSIEQ
                     TPQIQGVNDGQDARSFR"
     gene            complement(1821..2363)
                     /locus_tag="PKH81_00100"
     CDS             complement(1821..2363)
                     /locus_tag="PKH81_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ96477.1"
                     /translation="MFEHKTGLDANKDEDFRAIYSATMPILFKVAYRIAGTEEAAEDL
                     CHDALIKMTEKNMLFPSINDAKYWLIRVVKNASLNYAKRKGRERRAYERALKEDKRVI
                     DSGETVLLKEDTVQRVQAALEKLPDNLKAVLELKEYGELNYKEIGRVLGITEGNVKVR
                     VFRAREQLSRLIGADDVYMP"
     gene            complement(2356..3858)
                     /gene="cysS"
                     /locus_tag="PKH81_00105"
     CDS             complement(2356..3858)
                     /gene="cysS"
                     /locus_tag="PKH81_00105"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNQ96478.1"
                     /translation="MPMELFNTMGRSLQEFIPIHAGKAGFYGCGPTVYNYAHIGNLRA
                     YVFQDTLSRTLSFLGYDVHHVMNITDIGHLANDADEGEDKMVASAKERGMSVLEIASY
                     YTEAFFNDTQRLNIRMPTTVCKATEHIPEMIELISRIEANGHTYQSGGNLYFDISTFP
                     EYGKLANLNLDDLKAGARIDVDPNKRNPYDFVLWFTNSKFENQALVWPSPWGRGYPGW
                     HIECSAMSIKYLGESFDIHSGGIDHIPIHHTNEIAQSEAATGKKWVSYWLHNEFLVMD
                     KGKMSKSSGNFLTLQHLIDQGYDPLDYRLFLLGGHYRSQLAFSWESMEGARNARKNMI
                     QRVAKIIEATKALPDAVTSDGTYTAPLATDYLDRFRVALEADLSTPRAIAELQGLIRD
                     SSLSPRTILATIEKMDEVLALSIISTAQTMLDKKQQMVQGPGADARIDALIIERSCAK
                     KDKNYARADEIRNQLSAEGIILEDGPSGTIWKRIDLEDTTGCNSPGDSIV"
     gene            3998..4909
                     /gene="murB"
                     /locus_tag="PKH81_00110"
     CDS             3998..4909
                     /gene="murB"
                     /locus_tag="PKH81_00110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNQ96479.1"
                     /translation="MSAHTTFKLGGPADVYIEPPVVEELCALIDYCFTQGVPVYIVGG
                     GSNILVSDQGIRGVVLSLSGLQGTELMSDTDRLLVRSLAGTPVDELSAWCCAQSLAGL
                     ERFAGLPGTVGGAVFMNARCYDLSISDRFFSADILYFTGGRCTLSTMAFTPGEWEYKK
                     SPFQSGRGEDPLLITRDSRIVVAATFRVERSLSASLEADRLSFIEDRKKKGHFRYPSA
                     GSMFKNSRAFGKPSGQIIDEAGLRGFTIGGAQVAPWHGNFVINTGDARASDVRRLVSE
                     VQRQVLEKTGFLLEPEVVFTGEWSDLT"
     gene            4933..6048
                     /locus_tag="PKH81_00115"
     CDS             4933..6048
                     /locus_tag="PKH81_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNQ96480.1"
                     /translation="MRKRVIGIGFVSVLFLCATAWVCSAQVIGRIEYLEGTVEIVRDG
                     SPLRPIDIGSAVEHLDQIKTRANSTVCVSFLPSSGLSGQVDLEENTTAVLRSDSGGME
                     QVHELQLFTGALGVKVKRLASNKSSFRVRTPTAVLGVRGTEFTVISFNGAALVACKTG
                     EVRCDPWSSSAVSNGQAAPGDSALPGRLVEVLESQSVKTAEFPQGEFSSVWKDLRTRW
                     KTFQVELITANPVSFLNSFVPVWERSASQIEADAQRLRANSVLEKWLLEAKKGGPKGN
                     RSSWITERNAVMKDLLAIKPHLTVGMMSWYRLNELIPHLSSADMNKKLSNGQTVQAFV
                     NRFTKTETEVSSAFTLFYAAEKQYMLRNDGLSPFLEF"
     gene            6098..7357
                     /locus_tag="PKH81_00120"
     CDS             6098..7357
                     /locus_tag="PKH81_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ96481.1"
                     /translation="MLQLAFVNFRKGSYILVEGKVESDRFYIIQGGKVQITKDTEVVA
                     EEGGNILGPGDFIGVVSSMSGHSQIETAMAITDVTLISVRRDQFSELIEKNTPVAMKI
                     IYSFTRKMRYLDEALTRITLKKNVESDITHLFNIGEYYAKLSKYNLALYAYYHYLKNA
                     PNGPYAAVARERFMALKSMGVSVTTACLEPKPGEMTRLYTEETIVFCECQPGAELYII
                     QKGQVKITKIVDKNEVLLAVLKAGDMFGEMALLENKPRSASAIALEGTQLLAVNRQNF
                     NQMVSTQPQLIARLTTTLADRIWLMYKQLANTLITDPIGRMYDMLVIQLEKSKIPFQT
                     ARSYTFDFGPNELANMCGIVKEDAQRVISEFMREPIVRLTDERISVADMSELSKQNAY
                     RKKLMDLERARNESRSGGATTNSVVLW"
     gene            7409..>7897
                     /locus_tag="PKH81_00125"
     CDS             7409..>7897
                     /locus_tag="PKH81_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96482.1"
                     /translation="MKRFLSLVCSVLISLALFAQETSPAPQSSGQALSDTYRSITLGM
                     DIESVKEQLLSDGIFGYRGDRDVSLLPYKNRSLIETSGSSFIKRAFFQFYEEKLYIMI
                     FMIDVDRMDYYSLYTTFVTKYGEPTSLDPAKALWKDERVTVTLERPLTVKYVDSVVFQ
                     SLI"
BASE COUNT         1915 a         1793 c         1997 g         2192 t
ORIGIN      
        1 tccgctgctt taaggattat atcctgatgg cttgaaggga ctaagggatc ggcagcggta
       61 tatacaaggt aaagggggga ccgggtatcg gaaaagggtt tgaccagctg gctctgtatc
      121 ttggtagtga cgccataacc ggttaataca aaggtatggg aaatccgctc tgtttgtccg
      181 gattttatat tttcaagttg atgtagaatc gttccccgat aattgtccat gtatggcatg
      241 ggttttgatg cagtcagctc aatatccctt tgccaggtag ttgtttttgg aattggtaat
      301 cgtagataca ggatgtttgc ggcgcttgcc tgtatatcgg ttatatcaac ctgaagagaa
      361 atcgcgtagg tgcgggggct agtgtactct ttggtaccag gcttactgat aatggtgatt
      421 tcctgggaat tactgatacc ccgttctgtc tcaagaacaa gggatccagt agacgctcca
      481 tcaggcaccc gaatccttac ttcctgatcg ctccagaact cataatcaaa atcttgctca
      541 gaacaaggca ccattgagtc tttcttttga ttaacggcga gtattgcctg actgtcagca
      601 ttccaggtaa aaaggacacg ggagttgcct cgtgttaggc caaagttctt tccccgtaag
      661 gtcagtacct ttccaatttc gatcctgtcc tggcttatcg cctgaataac cggcatatcg
      721 ggcgtctgtg ttccacgccg ggtgatggga atgttttttt tgttggcgta catcaggggg
      781 ttacttctaa agcgaccacg ggatacatat acaagcccat ctttaacggt ttccggcaag
      841 gtcagcatga tggttgaatc attccaactg atataggcat tgccggaaat ccggttccct
      901 gcgatttcca cccatccatc gttcgctctc tgtccaaagt gtttgccttg taaaacgatt
      961 accgttcctg gctctcctat gggaggtaaa attgaaatga gggacggatt acggtgcgac
     1021 aagaggtaca ccgttgaact aagcagaaaa aaaactgcaa aggatacgag aatactgata
     1081 aatcgggcta tcggagagcc ctggagggtt ttacggagaa ttgagccgat catcgaaacg
     1141 aacgcgcgtc ctgtccgtcg ttcacgccct gaatctgcgg ggtttgctcg atgctgtcaa
     1201 aacgggttaa ttcggggagt cgaataataa caatatccgc atcacggaaa agatctgctt
     1261 cagagttaaa tctgcgcgca taattgagca tgctaaatat cttttttccg ctatccggca
     1321 tcagctcgtt cttgagttgg atagaaggaa tgtccggact gtaaatggac attgacgaaa
     1381 cgggaacgcg caagtcttca tcgttaaaag gagagatagt gtgtgcttga gtagcggcag
     1441 aagcatgaag gacatcgctc ggggagtttg aagagccacc tcgagcaacc cacacgggta
     1501 tacaaaccgc caacacaagg gccgcggcca gtgcagggag cggtaattgt attgaagcat
     1561 gaaaaaaacg agtggtggtt atggcattgc ccatcgactt tcgtgccttt cgttcgttca
     1621 ttttatcgta taagcgagct aaggatgcgt ccatctgctc agggctcaac agggccttat
     1681 catgatgcat ggtttggcta aggcgcaggt atgtatccgt ttttgaacgg catgcggggc
     1741 agttggaaag atgctggctg agtttttctt tccagggaga gggtacctct ccatcaacat
     1801 aggcagaata caaatcagca tcagggcatg tagacatcgt cagctcctat caatcgtgat
     1861 aattgttcgc gcgcccggaa taccctaacc tttacgtttc cttcagtaat gccaagcacg
     1921 cggcctatct ctttatagtt taattcgcca tattccttaa gctcaagaac agccttcagg
     1981 ttatctggca gtttttccaa tgccgcctgt acgcgttgga ccgtatcttc cttaagaagt
     2041 accgtctccc ctgaatcgat aacccgctta tcttccttga gcgctcgctc ataggcgcgc
     2101 cgctccctgc cttttcgttt tgcgtaatta agcgacgcat ttttaacaac gcgaattagc
     2161 cagtatttag cgtcgttaat cgaggggaac aacatgttct tctcggtcat ttttatcagg
     2221 gcgtcgtgac acaaatcctc ggctgcttcc tcggttcctg ctatccggta tgccacttta
     2281 aacaagatcg gcatggtagc tgaataaata gcccggaagt cttcgtcctt gtttgcgtct
     2341 aaacctgtct tatgctcaaa caattgagtc ccccggcgag ttacagcctg ttgtatcttc
     2401 caaatcgatt cgtttccaga tggtgccgga cggaccgtct tccagtataa taccttcggc
     2461 actcagttgg ttacggatct cgtcggctcg agcgtagttt ttatctttct tggcgcaaga
     2521 gcgttcgata atcagggcgt ctatacgggc gtctgctcct ggtccttgaa ccatttgctg
     2581 ttttttatca agcatggtct gagcggtgga aatgatgctt agggctagaa cctcgtccat
     2641 tttttcgata gtcgcaagaa ttgtacgggg cgaaagggat gagtctcgaa tcaacccctg
     2701 aagctcggca atggctcgtg gggtagagag atccgcttcc agggcaacac ggaatcgatc
     2761 aagatagtct gttgcgagtg gggccgtata cgtaccgtca gatgtaacgg catcaggaag
     2821 agcctttgtc gcttcgataa tcttggccac tcgctgtatc atgttttttc gtgcgtttct
     2881 tgctccttcc atggattccc aggaaaaggc gagttggctc cgataatggc cgcccaaaag
     2941 gaacaaacga taatcgaggg ggtcatatcc ctgatctatc aggtgttgca atgtcagaaa
     3001 attcccggaa gacttggaca tcttcccctt gtccattacc agaaattcat tgtgcagcca
     3061 gtatgaaacc catttctttc cggtggctgc ctcactttgg gcaatttcat tggtgtggtg
     3121 gatcggaata tgatctattc cacctgaatg gatatcaaag ctttccccca ggtacttgat
     3181 gctcattgct gaacattcaa tatgccaacc ggggtagccg cgaccccaag gcgagggcca
     3241 gaccaaggcc tggttttcaa atttactatt ggtaaaccag agaacaaagt cataggggtt
     3301 tcttttgttg ggatctacgt cgattcgcgc tccggcctta agatcgtcaa gattaagatt
     3361 agccaattta ccatattccg ggaaagttga gatatcaaaa tacaaatttc cgccgctttg
     3421 gtaggtgtga ccgttggctt cgatacggga aatcagttca atcatctccg gaatatgctc
     3481 tgttgccttg cagacagtag tgggcatgcg gatattaaga cgctgagtat cattaaaaaa
     3541 agcttctgta taataggaag cgatctccag gacagacatg ccccgttctt tcgcagaagc
     3601 gaccatttta tcttcacctt catcggcatc gttggcaagg tgtccaatgt cggtgatatt
     3661 catcacatgg tgtacgtcgt atcccaggaa ggagagcgtg cggcttaagg tatcctgaaa
     3721 aacatacgcc cgcagattac cgatatgggc atagttgtat acagtgggac cacatccata
     3781 aaagccggcc tttcccgcgt ggatggggat aaactcctgc aaacttcgtc ccatggtatt
     3841 aaaaagctcc attggcatgg tatcctccgc cctcgtattt tcgcgtttcg tgctgtataa
     3901 tgccggaacg atgaatactg tacggaaatt atgggaaaat agcaatataa gcgccatctg
     3961 cccccgctcg gctcacaccg ttttgtttga cgaaccgatg tctgcgcata ctacctttaa
     4021 gttaggcggg ccggctgatg tatatattga acctccggtt gtagaagagc tttgtgccct
     4081 tatcgattac tgttttacac aaggtgtgcc ggtctatata gtgggtggag gctcaaatat
     4141 cctggtgtca gatcagggga ttcggggcgt agtgctgtct ctgtccggtt tacaaggtac
     4201 tgagttgatg agcgacactg atcgtctttt agtgcgctct cttgcgggaa ccccagttga
     4261 cgagctttcg gcttggtgtt gcgctcagtc cctagccggt cttgaacgtt ttgcgggctt
     4321 accgggaacg gtcggtggtg cggtctttat gaatgcgcgt tgctatgacc tgtcgatttc
     4381 ggatcgcttt tttagtgcgg atatcctgta ttttaccggt ggacgctgta cactctcaac
     4441 gatggccttt acccccggtg aatgggagta caaaaaatcg ccgtttcaaa gtggacgagg
     4501 agaagatccc ctgttaataa cccgggattc tcgtattgta gttgcggcga cttttagggt
     4561 agaacgatct ctttcggcga gtcttgaagc cgatcgtctg tcttttatcg aggatcgtaa
     4621 aaaaaagggg cattttcgct atccttcggc gggcagtatg tttaaaaata gccgtgcctt
     4681 tgggaaacct tccgggcaaa ttattgatga ggcaggctta cgcggtttta caatcggagg
     4741 ggctcaggta gcaccctggc atgggaactt tgttataaat acgggagatg cccgggccag
     4801 tgatgtacgg cgtctcgttt ccgaggtaca gcgccaggta ttggaaaaaa ccggttttct
     4861 tttagaacca gaggttgtgt ttactggcga atggtctgac ctgacatgaa agtaacaaat
     4921 gggggagtat ttatgagaaa acgtgttatc ggaatcggtt ttgtatccgt tttgttttta
     4981 tgcgcgactg cctgggtttg ttctgcacaa gtaatcgggc gtatagagta ccttgaggga
     5041 acggtggaaa ttgtccgcga tggtagtccc ctcaggccaa ttgatatcgg ctcggcggtg
     5101 gaacatctgg atcaaataaa aaccagggcg aatagcacgg tctgtgtatc atttcttccc
     5161 tctagcggct tgagcgggca ggtagatctg gaagagaaca cgactgcggt gttaagaagc
     5221 gacagcggtg gtatggaaca agtacatgaa ctacagcttt ttaccggcgc tcttggcgtt
     5281 aaggttaaac gacttgcttc caataagtct tcctttaggg ttagaacccc aactgctgtg
     5341 ttgggggtaa ggggtacgga atttaccgta atcagcttta atggcgccgc ccttgttgcc
     5401 tgtaaaaccg gcgaggttcg ctgtgatccc tggtcatcaa gtgctgtttc aaacggtcaa
     5461 gctgcaccgg gtgactcagc cttacctggc cgtttggtcg aggttctgga gtcgcaatcg
     5521 gttaaaacgg cagaatttcc ccagggagag ttttccagtg tctggaaaga ccttcggacc
     5581 cgatggaaga cctttcaagt tgagctgatt accgctaacc cggtgtcttt tttaaactcg
     5641 tttgtaccgg tttgggagcg ttctgcttcc cagatagaag ccgacgcaca gcggttacgg
     5701 gctaattccg tcctggaaaa gtggcttttg gaggcaaaaa agggaggccc caaggggaac
     5761 cgatcttcct ggattaccga aagaaacgcg gtcatgaagg atttactggc catcaagccg
     5821 catcttaccg ttggaatgat gagttggtat cgcttgaatg agcttatccc gcatctgagt
     5881 tctgccgata tgaataagaa gctctccaac ggacaaacag tgcaagcttt tgttaatcgg
     5941 tttacaaaga cggaaaccga ggtttcatcg gcctttaccc tgttttacgc ggcggaaaag
     6001 cagtatatgc tcaggaatga cgggctttct cccttcctgg agttttaatt gacattgtag
     6061 gaagtcaaaa ctatactgag gtaacgggtg gtgctgattg ttacagctag cgtttgttaa
     6121 ttttagaaaa ggttcatata tcctcgtcga agggaaggtc gaatcggatc ggttttatat
     6181 catccaggga ggcaaggtcc aaatcaccaa ggataccgag gttgttgccg aagaaggtgg
     6241 caatattctt ggtcccggtg actttattgg cgttgtctcc agtatgtccg gtcatagcca
     6301 gatagaaacc gccatggcca ttaccgatgt taccctgatt tctgttcgcc gggatcaatt
     6361 ctcggagctg atcgaaaaaa acaccccggt tgccatgaag attatctact catttacccg
     6421 gaaaatgcgg tacctggatg aggctctcac tcgtattacc cttaagaaga acgtagaaag
     6481 cgatattacc catcttttta atattggcga gtactatgca aagctttcca aatataattt
     6541 agccctgtat gcctattacc attacttgaa aaacgcgcca aatggtccct atgccgccgt
     6601 tgcccgtgaa cggtttatgg cacttaaaag catgggtgtt agcgttacca ctgcctgcct
     6661 tgagccgaag cctggcgaga tgacccgtct atacactgaa gaaacgattg ttttctgcga
     6721 gtgtcagcct ggcgcggagc tctatatcat tcagaaaggg caggtcaaga tcaccaagat
     6781 tgttgataaa aacgaggtgc ttcttgccgt tcttaaagcc ggtgatatgt ttggcgaaat
     6841 ggccttgttg gaaaacaagc ctcgttccgc cagtgctatc gcccttgagg gaacacaatt
     6901 gctggcggtt aaccggcaaa actttaatca gatggtttcc acccagccac agctgattgc
     6961 tcgccttact accacccttg cggaccgcat ctggctgatg tacaaacagc ttgcgaatac
     7021 cctgattacc gatcctattg ggcgaatgta cgacatgctc gtcattcagc tggaaaaatc
     7081 aaagattccc tttcagacag ctcgatcgta tacctttgat tttggcccga atgagcttgc
     7141 aaatatgtgc ggtatcgtta aagaggatgc tcagcgggta atcagcgagt ttatgcgaga
     7201 gcctattgtt cgtctcactg atgagcgtat atctgtagca gatatgtccg agctgagtaa
     7261 acaaaacgcc tatcgaaaaa aacttatgga tttggagcgc gctcgaaatg aatcacgctc
     7321 gggcggggca actaccaaca gcgttgtctt gtggtagctt tcctatgccc tgcttcagtt
     7381 ccaaagcgat tacgccgaaa agaatagcat gaaacgtttt ctctcccttg tttgctctgt
     7441 ccttatatct ctcgccctct ttgcacagga gacttctcct gcccctcaat catccgggca
     7501 ggccttaagc gatacgtatc gatcgattac tcttgggatg gatattgaaa gcgttaagga
     7561 acagcttctt tctgacggga tctttggcta tcggggagac cgtgacgtat ccttattacc
     7621 ctacaagaat cgaagcctta tcgaaacatc aggttcatct tttattaaac gtgctttttt
     7681 tcagttttat gaagagaagt tatatatcat gatttttatg atcgacgtgg atcgtatgga
     7741 ctattattcc ctgtatacga cctttgttac taaatatggt gagccgacct cccttgatcc
     7801 agctaaggct ttgtggaaag atgagcgagt taccgtaacc ctggaacgtc cgcttacggt
     7861 caagtatgtg gattcggtgg tttttcagtc cctgatt
//
LOCUS       DAOQXQ010000004         7696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_9911_length_7696_cov_3.092789, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000004 DAOQXQ010000000
VERSION     DAOQXQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_7696_cov_3.092789"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2584
                     /locus_tag="PKH81_00130"
     CDS             <1..2584
                     /locus_tag="PKH81_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ96483.1"
                     /translation="ARECGLTSWTGLTGKNLADLPGIDVPLIKRMIEDLILNERPVVR
                     TLKTNAPDGSIRWYHADLRIIRSSAGVYGYILTKQDITGQIQPRAILESLMDTSADLI
                     AIVNPEGRLMYASQALARMTGVSSWHILIGHEWQYIFANMGAKGKRYHDFFEALTEGA
                     PQKMITMDLPDGRQVLSCRVNNLSYQDEPFGAVAMASNLTELVEARERAEDAVRAKAV
                     FLANMTHELRTPMNAVIGMNELLSRTSLAPLQRNYVSHIRSSATLLLAIIGDILDFSR
                     IEAKKMILNPQPFSTLSLVRDVINLVAVKVNEKELSFTVDLDPSLPDTLIGDETRIKQ
                     VFINLLNNAVKFTKKGSVSLTLMASPANNKRDISLTVMVKDTGIGIPRERQAELFKEF
                     SRIEGSSSSPIEGSGLGLSICKGLVTLMGGELDLESEEGLGTTFTARITLPFGKLARP
                     LAEFPDSRDIRLLVFERDDAAVRSIRAMTRAAGAEAIYCTSAYEFSAWLSRPGFDRTH
                     VIFEYSSAYQLVLNTADTLPGVSWLALLSLSDFLGSGKDPRISFIFKPLLVADFARFL
                     RGEGVDFTNSLPMTSTLGVPSRYFHARDVKALVVDDNAVNRKVAEGFLTALDIQVGEA
                     DSGEAALKKAAEKQFDLVLMDDVMPGMSGSETTVLMRKLKGYQDTPIIALSANAGEEF
                     EKRYRDAGMTDTLTKPIEFDSFVHCLRRWIPEERVTDVDTGDACALPVEQAKKDDTWI
                     EGLDREVGLSYTGSEKNLEMILKVFIRTGPSLMEKLESAHAGGDPAVLRIAVHSLISA
                     CANIGAMEVSSLAREAESAILKGDTEAVHTLYVRLHDALKTVLSGVGAYITKANAGKP
                     EGTT"
     gene            2581..3321
                     /locus_tag="PKH81_00135"
     CDS             2581..3321
                     /locus_tag="PKH81_00135"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNQ96484.1"
                     /translation="MKTGIIGAVAQEVELLFEALSASGHPVKISRKGSLEFREGVLSG
                     ERVVVVCCGVGKVNAALCAQILISAYAVERIVNTGSAGALDERLAVYDLVVSTDLVQH
                     DFDTTAFGYEPGRIPGMDSLSFAADPLLHEIALVAFMALREHRPDSNVELKMLEGRIA
                     SGDVFVADEKLRERIRDVFSPLCVEMEGAAIAQVCQVSEIPFVIIRSVSDLAGSEATV
                     SYEDFSREASRVSARLVIAMMTALAERS"
     gene            3325..3813
                     /locus_tag="PKH81_00140"
     CDS             3325..3813
                     /locus_tag="PKH81_00140"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HNQ96485.1"
                     /translation="MKEARNVESFSLDHTRVVAPYVRLAGIKSGPHGDTVSKFDIRFC
                     QPNECYMGTAGMHTLEHLMAEYIRDEIDGVIDLSPMGCRTGFYLSVFGEPGEKEIADR
                     VRATLVRVASWQDTEEVPGVDPVMCGNWKDHDLAEAKTWARKWIEGIDRKGFQAMPTL
                     TL"
     gene            complement(3803..5203)
                     /locus_tag="PKH81_00145"
     CDS             complement(3803..5203)
                     /locus_tag="PKH81_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNQ96486.1"
                     /translation="MTHTATSEQLVEAQRAYFNSGATRPVAFRIEQLTRLKKAILARE
                     KEIIAAAKADLGKGDFEAFTTEMVLVLYEINYVIRKLHGWAKARTTSPGLFNLPSKAR
                     VHHDPHGVCLIMSPWNYPFQLSLMPVVGAIAGGNTIILKPSAYSPATSHLVATLIGDT
                     FPREYIAAIEGGRQVNQDLLSCRFDYIFFTGSVAVGKLVMESAARHLTPVTLELGGKS
                     PCIIDKSADIDMSAKRAAWGKCINSGQTCVAPDYFLVHQDVHEAFIKRFLFYVERFYG
                     KDPHQNDEFPRIINEHHFKRLCTHITEAQKAGGTLAGGGRNNPEALKIEPTVIDGVSW
                     DSPLMQEELFGPIAPIITWTDEKQLIQKILDRPRPLALYLFSTDSKQQKRIMAAIPFG
                     GGCINDTIMHVATHTVPFGGTGESGMGGYHGKASFLTFTRSKPVIEKPFAIDVPLRYA
                     PFAGKYRFWRKFLFTR"
     gene            complement(5200..6324)
                     /locus_tag="PKH81_00150"
     CDS             complement(5200..6324)
                     /locus_tag="PKH81_00150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNQ96487.1"
                     /translation="MNTVHTIEHSASVTVNQVLALKKGEQFLILTNPDGDQFEISMAL
                     YRAAQDAGAEVTLIIQEEKTQLDFANQAALAAFGAHPDVCASISANKMGKDRQAIANP
                     WIGKDGKKIDHIFHYLMDEQKTLRAIWTPGITVDMFRRTAGIDYELLQRRCDAIIKAM
                     AGAESIHVSAPGGTNITVPITGRTAMSDDGKFTTPGAGGNIPAGEVFISPLPGQSEGV
                     IVFDGSISLNDGDMIVENPVRVVYSGGYITDISGGKEAELLKASIAAGEKKAREFEAE
                     GKLAKGEGEEYARNAKGLGELGIGLNPRAEIRGNMLEDEKAFHTCHFAIGSNYNNDAP
                     AMIHLDCLVKNPTITVNFPDGASVVIEKEGILVAPFGEIE"
     gene            6387..7115
                     /locus_tag="PKH81_00155"
     CDS             6387..7115
                     /locus_tag="PKH81_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNQ96488.1"
                     /translation="MNIILFEGERVFYRSDDRYTHIRKILKKGPGDTFRAGLIDGPEG
                     LAVIESVDEQGLAFSFQERIPPRPLFPLWMIIGFPRPIQLKRLLRDLSGLGVSRICLT
                     GTALGEKSYRDSSLVSRGAAREALIEGCMQAGGTVLPILEKAETVQEAIALYTDAASR
                     VLLDNVSPAGSLASYSFPSRGACGISLAVGSERGWTDDERALFAQNGYSSCSMGKRIL
                     RTETAACAAASITLAVSGLMERDV"
     gene            7112..>7696
                     /locus_tag="PKH81_00160"
     CDS             7112..>7696
                     /locus_tag="PKH81_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96489.1"
                     /translation="MNQDHIKDMLLALENTELDFTVTMTGKKSRKVNGLYKPDSREIL
                     LHNKNFTDDNQLVYTAIHEYAHHLQCEVDGPLRSPRSHTGAYWARFHALLEKAEKKGL
                     YSIGVEKSDSLSRLTEEIRSKYLAENGRLMNELGGLLAKGHALCQEAGVRYEDWIDRV
                     LCLPRTAAKTAVRVSSLDINPSLGYEAMKIVASQS"
BASE COUNT         1775 a         1731 c         2114 g         2076 t
ORIGIN      
        1 tgcgcgggaa tgcgggttaa cctcctggac cggattgacg ggcaagaacc ttgcagacct
       61 acccggtatt gatgttcctc tgataaaacg catgatagag gatctaatcc tgaatgagcg
      121 accagtagtt agaaccttga agacaaatgc ccccgatggt tctatccgat ggtatcacgc
      181 tgatttacgg ataatcagat cttccgccgg tgtgtatgga tacattctga ccaagcagga
      241 tattaccggt cagattcaac cacgcgctat tcttgagtct ctgatggata caagtgcaga
      301 tttaatcgcc attgttaatc ctgagggacg actgatgtac gcgagccagg ccttggcgcg
      361 tatgaccggg gtttcaagtt ggcatatcct tattggacac gagtggcagt atatttttgc
      421 aaatatgggc gcaaaaggta aacgctatca tgattttttt gaagccttaa ccgagggtgc
      481 gcctcaaaaa atgataacca tggatctacc tgatggaaga caggtcctga gttgtcgggt
      541 taataatctt tcgtatcagg atgaaccgtt tggcgcggtg gctatggcgt ccaacttgac
      601 ggagttggtg gaagcgcggg agcgcgcaga ggatgctgtc agggcaaaag ctgttttcct
      661 ggccaatatg actcatgagc tcaggacgcc gatgaacgcg gttatcggta tgaacgaact
      721 gttaagtaga accagtttag cgccattgca gagaaactat gtgtcgcata tccgctcttc
      781 tgcaaccctt ttgttggcga taatcgggga cattcttgat ttctcgcgaa tagaagcaaa
      841 gaagatgatc ctgaatccac aacccttttc taccctttcg cttgttcggg atgttatcaa
      901 tcttgtggca gttaaggtaa acgaaaaaga attgtccttt acggtagacc ttgatccttc
      961 gttaccggat acgctgatag gggatgagac acgcattaag caggtcttta tcaatctgct
     1021 taacaatgcg gtaaagttta ccaagaaggg ctcggtaagt ttgaccttga tggcatctcc
     1081 tgccaataat aagcgtgata tttctttaac ggttatggta aaggatactg gtattggcat
     1141 cccccgcgag cggcaagctg agttgttcaa ggagttttcc cgaatcgaag gctcttcttc
     1201 atctccgatc gagggctccg gtttagggct ttctatctgt aagggtttgg taacccttat
     1261 ggggggtgag cttgatctgg aaagcgagga aggcttgggc acaaccttta ccgcacgcat
     1321 taccctgcct tttggtaaac ttgcgcgacc gcttgcagaa tttccggact cacgtgatat
     1381 acggcttttg gtgtttgagc gcgatgatgc ggcagtccgt tcaattcgcg ctatgacacg
     1441 cgcggcgggc gcggaggcaa tttattgcac ctctgcgtat gagttttccg cctggctttc
     1501 ccggccgggc tttgatcgaa cccatgttat ttttgaatac tcaagcgcgt atcaacttgt
     1561 gctcaatacg gccgatacat tgcccggggt ctcatggctt gcgttactgt ccttgtccga
     1621 tttcttgggt tcggggaaag atccccgcat atcgtttatc tttaagcccc tgcttgtggc
     1681 agactttgcc cgcttcctgc gtggcgaagg agtggacttt accaatagcc ttcccatgac
     1741 atctacactg ggggtcccgt cccgttactt ccatgcacgc gatgttaaag cccttgtcgt
     1801 ggatgataat gcggttaacc gcaaggtggc agaaggtttt ttaaccgcct tggatataca
     1861 ggtaggcgag gcggattccg gagaagcggc cctaaagaaa gctgcagaga aacagtttga
     1921 tctggtgttg atggatgatg tcatgccggg gatgagcggg agcgaaacta ccgtattaat
     1981 gcgtaagttg aaggggtatc aggatacacc gataatcgcg ctatctgcca acgcgggaga
     2041 ggaattcgag aagcgatatc gggatgcggg aatgaccgac acgcttacca agccaatcga
     2101 atttgactcc tttgtacatt gccttaggcg atggattccg gaagagcgcg ttacagatgt
     2161 ggatacggga gatgcctgtg ccttaccagt tgaacaagca aaaaaagatg atacgtggat
     2221 tgagggtctt gaccgggaag ttggattgag ctatacggga tcggagaaaa accttgagat
     2281 gattcttaag gtgtttattc gcaccggccc ttctcttatg gaaaagcttg agagcgccca
     2341 tgctggagga gatcctgcgg tgttaagaat cgccgttcat tcactgataa gcgcctgtgc
     2401 gaatatcggg gcgatggaag tgtcttctct tgcaagggaa gctgagagtg cgattttgaa
     2461 aggcgatacc gaggcggtac atacactata tgtgcgcctt catgacgcgc ttaaaacagt
     2521 actctccgga gtcggtgcgt atattacaaa agctaatgcg ggtaaaccgg agggtactac
     2581 atgaagacag gcataattgg ggcggttgcc caggaagtag agttgctctt tgaagcgctt
     2641 tcggcttccg gacacccggt taaaatctcg cgaaagggtt ccctggaatt ccgcgagggg
     2701 gttttatccg gagagcgggt agtggttgta tgttgcgggg tcggaaaggt taatgcggcg
     2761 ctctgcgcac aaattcttat ttctgcgtat gcagtagagc ggattgttaa taccggatcg
     2821 gcaggcgccc ttgatgagcg tcttgcagtg tatgacctgg ttgtgtctac cgatttggtc
     2881 cagcatgatt ttgatactac tgcctttggg tatgagccag ggagaatccc gggaatggac
     2941 agtctttcct ttgccgctga tccccttttg catgaaattg cgcttgttgc gtttatggcc
     3001 ttgcgggagc atcggcccga ctcaaatgta gagcttaaga tgcttgaggg cagaatagct
     3061 tcgggggatg tgtttgttgc ggacgagaag cttcgcgagc gcatcaggga tgtttttagt
     3121 cctttgtgtg tggagatgga aggggccgcg attgctcagg tgtgtcaggt tagcgagata
     3181 ccctttgtta ttatccgcag cgtatctgat cttgcgggca gcgaggcaac cgtgtcgtat
     3241 gaagattttt cgcgcgaggc ttcgcgcgtg agcgctcgac ttgtaatcgc gatgatgaca
     3301 gcgcttgctg aaaggagtta atggatgaag gaagcgagaa acgtagaaag ttttagcctg
     3361 gatcacactc gggtagtagc tccgtatgtt agacttgccg ggataaaatc cgggcctcat
     3421 ggagatacgg tgtctaagtt tgatatacgg ttttgtcagc caaatgaatg ctatatgggg
     3481 actgctggaa tgcataccct tgagcatttg atggcggaat atatccgcga cgagatagac
     3541 ggtgtaatcg atctttcccc aatgggatgc cgtacgggtt tttatttgtc ggtgtttggc
     3601 gagccagggg aaaaagaaat tgccgatcgg gtgcgtgcaa ctcttgttcg ggttgcgtca
     3661 tggcaagata ccgaagaggt ccccggagtt gatccggtga tgtgcggtaa ctggaaggac
     3721 catgatcttg ccgaggcaaa gacctgggca agaaaatgga tagagggcat cgaccggaag
     3781 ggcttccagg cgatgccgac ccttaccttg taaaaaggaa tttgcgccag aagcggtact
     3841 tgccggcaaa gggtgcgtac cgcaggggta catcaatagc aaagggtttt tcgataactg
     3901 gtttggaacg ggtaaaggta aggaaacttg ccttgccatg gtatccgccc atgccgcttt
     3961 ctcccgttcc accaaagggg acggtgtgag tggctacatg catgattgta tcgttgatgc
     4021 agcctccacc aaaggggatt gctgccatga tacgcttctg ttgttttgaa tctgtgctga
     4081 acaggtacag cgcaagtggc cttggccgat ccagaatttt ttgaattaat tgtttttcgt
     4141 ctgtccaggt gattattggg gcaataggtc caaacaattc ttcttgcatc aaggggctat
     4201 cccaggaaac gccatctata acggtcggtt caatcttgag tgcttccgga ttatttcttc
     4261 ctcctcctgc aagcgttcct cctgcttttt gagcctcggt tatgtgggta cataagcgct
     4321 taaaatgatg ctcatttata attcgcggga attcatcgtt ttgatgagga tctttgccat
     4381 agaaacgttc aacataaaat aaaaaccgct tgataaaggc ttcatgtaca tcctggtgga
     4441 ccaggaagta gtcgggcgct acgcaggttt gtcctgagtt aatgcatttt ccccaggccg
     4501 ctcgcttggc gctcatgtct atatccgcgc ttttgtcgat gatgcacggg ctctttccac
     4561 caagttccag ggtaacaggc gttaggtgcc gggctgcgct ttccataacg agtttgccca
     4621 ccgcaacgct accggtaaag aagatataat caaagcggca actcagtaga tcctggttca
     4681 cttgccttcc accttcgatg gcagcgatat actcacgggg aaaggtgtct cctataagag
     4741 tggcaacaag atgcgaggtt gcaggagaat atgccgatgg ttttaagatt atggtgttgc
     4801 caccggctat ggctccgacg acgggcatca gtgaaagttg aaagggatag ttccaggggg
     4861 acataatcag gcataccccg tgcgggtcat ggtgaactcg cgctttagag ggaaggttaa
     4921 aaagtccggg gctggtggta cgtgcctttg cccatccgtg aagcttgcgg attacatagt
     4981 tgatctcgta gagaaccagg accatttcgg tggtaaaggc ttcaaaatcg cccttgccca
     5041 ggtcggcttt tgctgccgca ataatctctt tttccctggc gaggattgcc tttttaagcc
     5101 ttgtaagttg ttcgatgcgg aatgccaccg gacgggtagc gcctgaatta aagtaggcgc
     5161 gttgcgcttc taccagttgc tctgatgttg cggtatgtgt cattcaatct ccccaaaagg
     5221 agcgacaaga atgccctcct tttctattac tacagatgca ccgtcaggaa aattgacggt
     5281 gatagtcgga ttttttacca ggcaatcaag atgaatcatt gccggggcgt cgttgttata
     5341 attgcttcct atggcgaagt ggcaggtatg aaaagctttt tcatcctcca gcatgttacc
     5401 gcgaatctcc gctcgggggt ttaagcctat gcccagttcc ccaagtccct ttgcgtttcg
     5461 cgcatattct tcaccttctc ccttggcaag ttttccttcc gcctcgaatt cccgtgcttt
     5521 tttctctcct gccgcgatgg atgctttaag aagttctgcc tccttgccgc cggatatgtc
     5581 ggttatgtat ccgcctgaat acacaacccg caccgggttt tccacaatca tgtctccatc
     5641 attaagactg atggagccgt caaagacaat taccccttcg ctttggccgg ggaggggact
     5701 tataaacacc tctcctgcgg ggatattgcc tccggctcct ggggtagtaa atttgccgtc
     5761 atcgctcatt gccgttcggc cggttattgg aacggtaatg ttggttccgc caggggcgct
     5821 aacgtgtatg gattcagctc cggccattgc ctttatgatt gcgtcgcatc ttctttggag
     5881 aagttcataa tcaatgcccg cggtacgtct gaacatgtcc acggtaatac cgggcgtcca
     5941 aatagcacga agcgtttttt gttcgtccat aaggtaatga aagatgtgat cgattttttt
     6001 tccgtctttg ccaatccagg ggtttgcgat ggcttgtcga tccttaccca ttttgtttgc
     6061 ggagatagac gcgcagacgt cgggatgggc gccaaaggct gcaagcgcag cctgattcgc
     6121 aaaatcgagc tgtgtttttt cttcctgaat aataagggtt acctcggccc ctgcatcttg
     6181 tgcggctcgg tacagggcca tgcttatttc gaattgatct ccatccggat tggtcaggat
     6241 aaggaattgt tctccctttt ttagggctag tacttgattg acggtaactg atgcagagtg
     6301 ctcgatggta tggacggtat tcatggataa aatgtacgcc cgtcggtagg aaatgtcaaa
     6361 cggctttggt atactcctta atacgcatga acattatcct gtttgagggt gagcgggttt
     6421 tttaccgttc ggacgaccgt tacacacata ttcgaaagat tcttaaaaaa gggccgggag
     6481 acacctttag ggcagggttg attgatggac ccgaaggtct tgcagttatt gagtcggtgg
     6541 atgagcaagg tcttgctttt tcttttcaag agcgcatccc ccctcgtccg ctattcccgc
     6601 tatggatgat aattggcttc cccaggccaa ttcagcttaa acgcttgttg cgtgatcttt
     6661 ccggtcttgg cgtttcccgt atctgtctta ctggtaccgc acttggagaa aagagctatc
     6721 gtgactcttc tttggtaagt agaggggccg cgcgcgaagc cttgatcgaa ggatgtatgc
     6781 aagcaggggg aacagtactc cccatacttg aaaaggcaga aaccgtgcaa gaggctatag
     6841 ccttatatac agatgcagcc tctcgggtgc ttctggataa cgtatcgccg gcagggtctc
     6901 ttgcctctta ttcatttcct tcccggggag cttgtggtat atcccttgcc gtggggagcg
     6961 agcgtggatg gacagatgac gagcgggcat tgtttgcaca aaacggttat tcatcgtgct
     7021 ctatggggaa aagaattctt agaacggaaa cagctgcgtg cgctgctgcg tccataacct
     7081 tggccgtttc gggtcttatg gagagagatg tatgaatcaa gatcacatta aggatatgct
     7141 cctcgcactg gaaaatactg aattggattt tacggttacc atgaccggga aaaaaagccg
     7201 gaaggttaat gggctgtata agcctgattc ccgggagata ctgctgcata acaaaaactt
     7261 tacagacgac aatcagcttg tgtataccgc gattcatgaa tacgcgcatc atttacaatg
     7321 cgaggttgat ggacccttac gctctccccg ctctcataca ggagcctact gggcgcgatt
     7381 tcatgccctg ctcgaaaaag ccgagaaaaa aggtctgtat tcaatcggcg tagagaagtc
     7441 cgattcgctt tcgcgcctta ccgaggaaat ccgctcaaag tatcttgcgg agaatgggcg
     7501 acttatgaac gagttgggag gactgcttgc aaaggggcac gccctgtgtc aggaagcggg
     7561 agttcgctat gaagattgga tagaccgggt gctttgcctt cctcgcactg ccgcgaaaac
     7621 agcggttcgg gtgagctccc ttgatattaa tccttccttg gggtatgagg cgatgaagat
     7681 cgttgcctcc cagtct
//
LOCUS       DAOQXQ010000005         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_9994_length_7641_cov_3.563274, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000005 DAOQXQ010000000
VERSION     DAOQXQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9994_length_7641_cov_3.563274"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="hprK"
                     /locus_tag="PKH81_00165"
     CDS             complement(<1..924)
                     /gene="hprK"
                     /locus_tag="PKH81_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNQ96490.1"
                     /translation="MAVKRFSVLDLLGLGLKEHDSLDLRCICGRRGLVRDITIPDLNR
                     PGLALSGFFDSFAYQRVQLFGRGEVAYLNKIAGEGRLDTIQQMFSYPLPCCIFTHHLE
                     PAKEFLDLAEETGCPILQTGLDSSELSSRLLRILSNIFAPKISIHGVLVEVYGLGILI
                     LGDSGVGKSETALELIERGHRLVADDVVEIACVNGNILTGQGANKIIGHHMEIRGLGI
                     INITQLYGVGSIRERKEVQLVAKLEEWDANKVYDRLGTETLTMEILGVQVPKLEIPVK
                     PGRNVPIILETAAMNERLKSMGYFSAKEFNQN"
     gene            complement(1023..1313)
                     /locus_tag="PKH81_00170"
     CDS             complement(1023..1313)
                     /locus_tag="PKH81_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNQ96491.1"
                     /translation="MNINVQAVKFTMDEEQREFVDRKFERIKYAEELITDVLCTIKYD
                     KKFSCDTTVNFRWGGNAHVGAEDFDFGAVVNKMIDVLDLKIKKEKDKVQEKK"
     gene            complement(1395..2828)
                     /gene="rpoN"
                     /locus_tag="PKH81_00175"
     CDS             complement(1395..2828)
                     /gene="rpoN"
                     /locus_tag="PKH81_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNQ96492.1"
                     /translation="MQGQRQELIQQQRLTLSPQLVQSIKLMAMPFADLRERIIDEVEK
                     NPALEVVSDPFETAMPRPPREHYTVRQGVAQGGGDEESDGHKDFIEGVLQRDATLQDY
                     LLSQLGELSLNPAVSDLASLIIQNLDRDGFHQVPPQELPGGHDPVLLQAALETVRSLD
                     PEGCATTDFRESLVVQAKLLRRLAPAGKKDELLETVITILEKYFSALEKGRPDALVKA
                     LARIGKPAPQLDVETAGDVFDLIRTLDPFPGRSHASDMHSYVVPDVIVNRTEDGYSVV
                     INEEEIPVLGLSPFFMDLEGLETDKAARDFARESVKEARWFLSTLERRNLTILKLARA
                     LIVFQGDFFAHGPSHLAPLRMKDVAEEVGLHEATISRAANGKYLQCEWGLFELRYFFS
                     NQVGSLHASASRSSSTQSSKDNPYSAGRFSKQGVKEIVREIVESSSENLSDQKIADQL
                     ALRGIHIARRTVAKYRNELTIKSSFER"
     gene            2905..4239
                     /locus_tag="PKH81_00180"
     CDS             2905..4239
                     /locus_tag="PKH81_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNQ96493.1"
                     /translation="MKDTNPLAPYALTIPNLLIPDTNIDLSRWSVIACDQFTQDREYW
                     AKLKNEVGDQPSILNIILPEVYLEDSDRAERLANIRSAMNDYLNPQRSGGPVFRSESR
                     GMVYIERITAYGRCRKGLIACVDLDGYDWKPDSRAPVRATEATIVDRIPPRMEIRRGA
                     PIESPHIMLLVNDPGKTLVEEAGKAAHNKEILYSTTLRPQSGSITGWLIDSDQEIRNI
                     GQALEAIKQANTDTDGSSFIFAVGDGNHSLATAKAVWDEYRQNCERTGITIDPNHPAR
                     FALVEIVNLYDEGLTFEPIHRVLFGTDTATLSTWLTAKMGGTLKSIENAHKLEEAVAE
                     QGHTRFGFISPEGMTILDTPGTTLAVSRLQPLLDEFLSTHPGTSIDYIHGTEEALRLG
                     TKAGTVSMLLPPVEKDSFFTTIASGGPLPRKSFSMGEASEKRFYLECRRLFS"
     gene            4320..5147
                     /locus_tag="PKH81_00185"
     CDS             4320..5147
                     /locus_tag="PKH81_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ96494.1"
                     /translation="MTAEYTFDENRTELMNRCALPILLVLTALAIGISILALASGYVT
                     VFQNLFYFPILVASISCPRKGLYFTTACSALYFILIIPVFGYYNEIAAALIRVFFFEL
                     VAVVVGYLSWKRQKAENALKEQLANLNDIVQRQTEYIKMELDDARRLETAYRSETGYH
                     EKIIDGAAVAILNWNPEGYIIKANPAVCDFLRIRESELLGRRITAILPLDESGLTTYP
                     TYVETDIHTAGGQIAKALWVISRIEETAGEENPMVYMAIGVALPSRVLLEESRSGHR"
     gene            5134..5946
                     /locus_tag="PKH81_00190"
     CDS             5134..5946
                     /locus_tag="PKH81_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96495.1"
                     /translation="MDIANDLGKKVFFLYPPSVIRDELVSRLLEQEYEVYMLKDLSMA
                     NRLMVLYPDSLVFINIDTGLSEGEWEKWVRDTQQNPATDGIGIGIVSYNTDEDLQRKY
                     LMDIGIPCGFIKLKLGIEESTRILLATLKANEAKGRRKYVRASCEHDTISSINIREGP
                     IKSIGKIVDISAVGFSCILDPDPAFQKNTVLHDVQLKLRASLVRLTSVVVFGTRMKDD
                     RTMYVMLFSPKMEQLSRDKIRNYIQIALQAEIEKEADRLAAEEKEKKTAEAK"
     gene            5963..6319
                     /locus_tag="PKH81_00195"
     CDS             5963..6319
                     /locus_tag="PKH81_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96496.1"
                     /translation="MGKIRKFINILKVEIEDMQEDCEHLIEQSHKKREQGLYTDYVLM
                     ENVSVYRSQIDALTELVEKIEEEDWSGYNTVEEASNKITTIVPQLIKKGDFPEYTIDL
                     VSRKMEKILQFLELSC"
     gene            complement(6355..6438)
                     /locus_tag="PKH81_00200"
     tRNA            complement(6355..6438)
                     /locus_tag="PKH81_00200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6402..6404),aa:Leu,seq:caa)
     gene            complement(6478..>7641)
                     /locus_tag="PKH81_00205"
     CDS             complement(6478..>7641)
                     /locus_tag="PKH81_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ96497.1"
                     /translation="LVAGNVSLFSTEFSRWGYRALVFLVISCPCAFVISVPLGYFGGI
                     GGAARRGILVKGAQALDGLVKVKAVVFDKTGTLSEGRFSVTALSTAPSVSEQELLATA
                     ASIEKHSNHPIARAIVSHVNQKYPDSHPHSSVNPSDIAGSVCTERPGYGLVCGSGTNR
                     VAAGTKRLMDELGVSVGGLWDEHTRVYVSRGSDLLGCIVLSDRPKKDAFAAVAQLRSL
                     GVSRIALISGDSASSAHALASEVGIMEVYSGVLPDEKVHYFEQIARDSEQGSAIFVGD
                     GINDAPVIARADVGIAMGGIGSDAAIEAADLVIMNDNPRSVPEAIRLARYTRSIVYQN
                     IAFAFAVKLLFLILGAFGIATLWEAVFADVGVALIATANALRARSSRIPGDKR"
BASE COUNT         2180 a         1957 c         1742 g         1762 t
ORIGIN      
        1 gttttggtta aattccttgg ccgaaaaata ccccatactc ttgagccgtt cattcatcgc
       61 ggcagtttca agaatgatag gaacatttcg tcccggttta accggaattt ccagcttggg
      121 gacctgtaca ccaagaattt ccatggtgag cgtttcggtt cccaatcggt catacacctt
      181 gttggcatcc cattcttcga gctttgccac aagctggacc tcttttcgtt cgcgaataga
      241 gccgacccca tagagttggg tgatattgat aatacccaat ccgcgaattt ccatgtggtg
      301 accaataatc ttgttagcac cctgtccggt aaggatatta ccgttgacac aggctatctc
      361 gacaacatca tcagcgacca gtcggtgtcc ccgttcaatc agttcaagag cggtttcgct
      421 cttacctacg ccggaatcac ccaaaatcaa aataccaagt ccatacacct ctaccaaaac
      481 cccatgaatg gaaattttgg gagcaaaaat attggacaga atacgaagca agcgagatga
      541 taactcgctg gaatccagtc ctgtctgcaa tattggacac cccgtttcct ctgcgagatc
      601 aaggaattct ttagccggtt caaggtgatg ggtaaaaatg caacaaggca acggatacga
      661 aaacatttgt tgaatcgtat ccaggcgtcc ctctccggca atcttgttca gatatgccac
      721 ctcgccccgc ccaaaaagct gcactcgttg gtaggcaaag gaatcgaaaa atccggaaag
      781 agcaaggcca ggacggttta aatccgggat agtaatatcc cgaaccaggc cgcggcgacc
      841 gcaaatgcac cgtaaatcga gggaatcgtg ttccttaagg cccaatccaa ggagatcaag
      901 cacggagaag cgttttacag ccatacttga ctatacccct caggtggcgt gtctgtaaag
      961 agaagggccg ggactgcagg ggctcctgtg cccgctcggt gttcaccggc ccagggcagc
     1021 acttatttct tttcctgtac cttatccttt tcttttttaa tcttaagatc caaaacatcg
     1081 atcattttat taaccaccgc accaaaatca aaatcctcgg cgccaacgtg ggcattgcca
     1141 ccccaacgaa agttaactgt ggtatcacaa gaaaatttct tgtcgtattt aattgtgcag
     1201 aggacgtcgg tgatgagttc ctcggcgtat ttaatccgct cgaatttgcg gtccacaaac
     1261 tcccgctgtt cctcatccat ggtaaattta actgcctgta cattgatgtt catcttgaac
     1321 ctcccctcta tgatgtaaga cgcgcgatcc ggagcaaagc ctgatcgggt acgtcaatca
     1381 taatacggaa acgcttatct ttcaaatgaa gattttatag taagctcgtt tcggtacttg
     1441 gcaacggtcc gtcgggcaat atgaatccct cgcagagcaa gctgatcggc aatcttttgg
     1501 tcagagaggt tttcggagga agattcaaca atttcccgga caatttcctt aacgccttgc
     1561 ttggaaaagc gcccggccga atacgggttg tccttgcttg actgagttga gcttgagcgg
     1621 gatgcggaag catgaaggga gccaacctga ttactaaaaa aataccgtaa ttcaaaaagg
     1681 ccccactcac actgcagata ctttccattc gctgcacgag aaatggttgc ctcatgaagg
     1741 ccaacctcct cggccacatc tttcatacgc aaaggtgcca gatgcgaagg cccatgagca
     1801 aaaaaatcac cctgaaacac aatcagagcc cgtgcgagct taagaatagt caaattgcga
     1861 cgttcgagcg tagataaaaa ccaccgagcc tcctttaccg attcgcgggc aaaatcccga
     1921 gcagccttat ctgtttcaag accttccaaa tccataaaaa agggagaaag accaaggacc
     1981 ggaatctctt cctcgtttat caccaccgaa taaccatctt cggttcggtt aacaattaca
     2041 tcgggaacaa cataagagtg catatcgctt gcatgagagc gccccggaaa aggatcaagg
     2101 gtacgaatca agtcaaaaac gtccccagcg gtttccacat ccagttgcgg tgcgggcttg
     2161 ccaatacggg caagggcctt taccagggca tccgggcgac ccttctccag ggcagagaaa
     2221 tacttttcaa ggattgtaat aaccgtttcc agaagctcgt ctttcttgcc tgccggggca
     2281 agacggcgaa gaagttttgc ctgaaccacc aagctttccc taaaatcggt ggtagcacat
     2341 ccttcaggat caagcgaacg aactgtctca agggcagcct ggagtaatac aggatcgtgt
     2401 ccaccgggaa gctcctgcgg aggaacctga tgaaatccgt cgcgatccaa attttgaata
     2461 ataagcgagg caagatcaga taccgccgga ttaagcgaaa gctctccaag ctgactaagc
     2521 aggtagtcct gaagagtcgc atcgcgctga agtacaccct caataaaatc cttgtgcccg
     2581 tcgctttcct cgtcccctcc tccctgagcc acaccctggc gaaccgtgta gtgttcacgg
     2641 ggaggacgag gcatggcggt ttcaaaggga tccgagacaa cctcaagggc cggatttttt
     2701 tcaacctcat cgattatgcg ctcacgtaaa tcggcaaacg gcatagccat caatttgatg
     2761 gactgcacca attgcggact aagggtgagg cgctgctgtt gtatcagttc ttgtcgttgt
     2821 ccctgcatgc atctttagaa tacggaagaa cccccgcggg gtcaagattg accgtgaact
     2881 gcttttttgc gtatgatagc atccatgaaa gacacaaacc cccttgctcc ctatgctcta
     2941 acaattccca acctgttaat acctgatacg aatatcgatc tatcccgatg gtcggttatt
     3001 gcctgcgacc agtttaccca ggaccgcgag tactgggcaa agctgaagaa cgaggttgga
     3061 gaccaaccgt ctatcctgaa catcatcctt ccagaagtct atctggagga ttctgatcgg
     3121 gcggaacgcc ttgccaacat tcgatcggcc atgaacgact acctgaaccc acaacgctca
     3181 ggcggcccgg tattcaggag cgaatccagg ggaatggtgt atatagaacg aattactgcc
     3241 tatggaagat gccgcaaagg acttatcgcc tgcgttgatc ttgacggata tgactggaaa
     3301 ccggattcac gagctcctgt acgggcgact gaagcaacca tagttgatcg aatccccccg
     3361 cgaatggaaa ttcgccgtgg agcgcccata gaaagcccgc acatcatgct gttggtcaat
     3421 gatcccggca aaacacttgt ggaagaagcc ggtaaagctg ctcataacaa agagatcctg
     3481 tactcgacca cactcaggcc ccaatcaggc tcaataaccg gatggcttat cgactcggat
     3541 caagaaatcc ggaacatagg ccaggcgctt gaagcgatta aacaagcaaa taccgataca
     3601 gacggctctt cgtttatctt tgcggtaggt gacggaaacc actctctggc gacagccaaa
     3661 gcagtgtggg atgaataccg acaaaactgc gaacgcacag ggataacgat agacccgaat
     3721 catcccgccc gatttgccct ggttgagatt gttaacctct atgatgaagg gctaaccttt
     3781 gaaccaatac accgcgtact gtttggaacc gataccgcaa cactctccac ctggcttact
     3841 gcaaaaatgg gtggaaccct gaaaagcatc gaaaacgccc acaagctgga agaagcggtg
     3901 gcggaacagg gacatacacg ctttggtttt atatctcctg aagggatgac aatccttgat
     3961 acccccggaa caaccctcgc agtcagccgt ctccaaccac ttcttgatga gtttttaagc
     4021 acacatccgg gaacctcgat tgattatatc catggaaccg aagaagcttt gcggctcggc
     4081 accaaggcag gaaccgtgtc gatgttactc cccccggttg aaaaagacag cttttttacc
     4141 acaatcgcct caggcggacc gcttccccgg aaaagctttt ccatgggcga agcgagtgaa
     4201 aaacgctttt atttagaatg caggcgctta tttagctaaa tactgccggc tgcctgtaca
     4261 aggcagccgc tctcccaccg gaatttgcaa tccatcgtct cgtgtactac catgttcata
     4321 tgaccgctga gtacaccttc gatgaaaacc gtaccgaact aatgaaccgt tgtgctcttc
     4381 cgattctact ggtattaacg gctttggcta ttggcattag tatattagcc cttgcttccg
     4441 gctacgtaac ggtattccag aacctttttt attttccaat attggttgcc tcaatttctt
     4501 gcccccgtaa aggcctttac tttaccacgg cgtgctcggc actctatttt atcttaataa
     4561 tcccggtatt tggctattac aacgagatag cagcggccct tatcagggtg tttttctttg
     4621 aactggtagc cgttgtggtc ggctacctga gctggaagag acaaaaggca gaaaacgcgc
     4681 taaaagaaca attagccaac cttaacgata ttgtccaacg tcaaactgaa tatattaaaa
     4741 tggaacttga tgatgcccga aggctggaaa ccgcataccg cagtgaaacc ggctaccatg
     4801 aaaagattat cgatggagca gcggtagcaa tccttaactg gaatcctgag ggatatatca
     4861 taaaggcgaa tccagcagta tgcgatttct tgcgaataag ggaatcggaa ctgctcgggc
     4921 gcagaattac cgcaatctta ccccttgatg aatccggatt gacaacgtat cctacctatg
     4981 ttgaaacgga catccatacc gctggcggtc agatcgccaa ggccttatgg gttatttcca
     5041 gaatagaaga gaccgccgga gaggagaacc ccatggtgta tatggcaata ggggtcgccc
     5101 tcccttcacg ggtcttgttg gaggaatctc gtagtggaca tcgctaacga ccttggtaaa
     5161 aaggtctttt ttctctaccc acccagcgtc atccgggacg aattggtaag tcgattgctg
     5221 gagcaagaat atgaagtgta catgctcaaa gacctgtcga tggcaaacag gctcatggtg
     5281 ctatatccag actcccttgt gtttatcaat attgataccg gactctccga gggtgaatgg
     5341 gaaaaatggg ttcgcgatac ccagcaaaac cccgcgacag acggcattgg tattggcatc
     5401 gtgtcgtata ataccgacga ggaccttcag cgaaaatacc tgatggatat aggaataccc
     5461 tgcggattta tcaaactcaa acttggtatt gaagaaagca cccgaatact tcttgctacc
     5521 cttaaagcaa atgaggcaaa aggacggaga aaatacgttc gcgccagttg tgaacatgac
     5581 accatctcct ctattaatat tcgggaagga ccaattaaaa gcatcgggaa aattgtagac
     5641 atcagtgccg tcggattttc atgcattctt gaccctgatc ccgcctttca aaaaaacacc
     5701 gtccttcatg atgttcaatt aaaactcaga gcatcactcg ttcgccttac ctcggttgtc
     5761 gtattcggaa ctcgcatgaa agatgacaga accatgtatg tcatgctttt ctctccaaaa
     5821 atggaacagc tatcccgaga taaaatacga aactacatac agattgcctt acaagcagaa
     5881 atcgagaagg aagcggaccg attggcagcc gaagaaaaag aaaaaaagac tgccgaagcc
     5941 aaatagcgga caaggagagt gtatgggaaa gattcgcaag tttatcaaca tccttaaggt
     6001 agaaattgag gatatgcagg aagattgtga acacttgatt gaacagtcgc ataaaaaacg
     6061 cgaacaggga ctctacaccg actatgtcct tatggagaac gtgtctgtat accgaagcca
     6121 aattgacgca ttgaccgagt tagttgaaaa aattgaagaa gaagactggt cggggtataa
     6181 caccgtagaa gaagcatcga acaaaataac tactatagtg ccgcaactga taaagaaagg
     6241 tgatttcccg gaatacacca tagacttggt gagccggaag atggagaaga tactgcaatt
     6301 tttggaattg tcgtgttaat cagtaactgc tcgcaagatt gttggtgaat agaatgccgg
     6361 cgatcggact cgaaccgata cacccgtgaa ggcggcagat tttgagtctg ctgcgtctac
     6421 caatttcgcc acgccggcat agaactatta caaagtatca aaaaaacgcc agtctgtcta
     6481 tcttttatcc ccaggtattc gtgagcttcg tgcccgtaag gcatttgccg tagcaatgag
     6541 cgccacgcca acatccgcaa acactgcctc ccataaggtt gctatcccaa aagctcctag
     6601 tatcaaaaaa agtagcttaa cggcgaaggc aaaggcaatg ttttgataca caatactgcg
     6661 agtatatcga gccaagcgaa tagcttctgg caccgaacga ggattgtcat tcataataac
     6721 caggtcagca gcttcaatcg cagcatcact tccgatccct cccatcgcaa tacccacatc
     6781 cgcgcgagcg ataaccggag cgtcattaat cccgtctccg acaaaaatcg cagaaccttg
     6841 ttcagagtcc ctggctatct gttcaaaata gtgaaccttt tcatcgggaa gcacccccga
     6901 gtacacctcc ataatcccta cttccgaggc cagtgcatga gctgaagacg cggaatcgcc
     6961 acttatcaat gctattctgg aaacaccgag ggaacgcaat tgtgccactg ccgcgaaagc
     7021 atccttttta ggtcgatctg acaacacaat acagcccaac aaatcagaac cacgcgatac
     7081 atatacccga gtgtgctcat cccacaaacc gccaacagaa acccccaatt catccataag
     7141 tcgcttggtt cctgccgcca cccgattagt cccagaacca cacacaagcc catacccggg
     7201 tcgttccgtg cacaccgaac ctgcaatgtc agaaggattt actgatgagt ggggatgaga
     7261 gtcaggatac ttctggttaa catgagatac aatcgcgcga gcaatcggat gatttgaatg
     7321 cttttctatg gaagctgcag tggcaagaag ctcctgttca ctgaccgaag gtgcagtaga
     7381 aagcgcagta accgagaaac ggccttcgct caaggtgcct gttttatcaa aaacaaccgc
     7441 cttaaccttt accagtccat caagggcttg cgctccttta actaaaatgc cccggcgcgc
     7501 ggctccccca atcccgccaa aatagccgag tggaacagaa ataacaaagg cgcatgggca
     7561 ggagataacc aaaaaaacca atgcacgata gccccaccgg gaaaattccg tactaaagag
     7621 agacacattt cctgcaacca a
//
LOCUS       DAOQXQ010000006         7499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_10239_length_7499_cov_3.430548, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000006 DAOQXQ010000000
VERSION     DAOQXQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10239_length_7499_cov_3.430548"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1038
                     /locus_tag="PKH81_00210"
     CDS             82..1038
                     /locus_tag="PKH81_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ96498.1"
                     /translation="MFVEPLVRVEGLTKKFNLEAGFFARFGKFVHAVNDLSFTIGKGE
                     TYALVGESGCGKTTTARLIVRMYERDAGTILFGRDTDVGLLRGRDLRSYREKVKYVFQ
                     DPARSLNPRMSVYNVLTSGYRWSSRWPGARRAREEAALILEEVGLAPSDLDRRPADFS
                     GGQRQRISIARSLLMQPELLICDEVVSALDVSIQGQILNLLQDIREKRGLSILFIAHD
                     LKVASYFCDRIGVMYRGELMEEGRAQDLYRNHGHPYTQLLFASATGGTEEAPGSVTTP
                     QWNSAHGCAFAHRCPLADNSCYAQRPEWKNIGDDHFIRCHKR"
     gene            1061..1924
                     /locus_tag="PKH81_00215"
     CDS             1061..1924
                     /locus_tag="PKH81_00215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ96499.1"
                     /translation="MSGARKATRPTAAILYDFDKTLCTRDMQEYSFIPGLGLSAQEFW
                     SEASRLSEQGMDPILAYMYLMLKKSREAEQPIRRNDFVSMGKNIAFFPGVLEWFNRIS
                     SYGKSAGLEVEHFIISSGLTEIIEGSSIGAQFNKIYACAFHYDVNGVADWPLVSVNYT
                     TKTQFLFRINKGVLDGRDGEGLNRFVPEDERPVPFRNMVYIGDGMTDIPCMKLVKTNG
                     GHSIAVYPKDQKEKAAPLLRDKRVDFIASADYRADKELDRLVKKILDRISVVHMLNES
                     NRAQRQSLGKV"
     gene            1921..2730
                     /locus_tag="PKH81_00220"
     CDS             1921..2730
                     /locus_tag="PKH81_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNQ96500.1"
                     /translation="MRYNEPGPVDAVGAFLIAVVYILLAGILAQLPALSPLIIFFPLG
                     FALFSFVYAAARSFYLPSVFPLVRPTLKQVAGATLMIVGILCVITFLMITVQAIFPSF
                     SPDAIDAEEVLSKGLLSGIIGVALLPAVSEEILCRGLMLKALRGSVPRLVAIVVSNLL
                     FAVLHFDIYRIPFTFVSGLVITWVAYETASLVLPVIMHFLHNMILFVIAWSIDRYVKP
                     EAMAQEGLETLAASDPRLLWFSLLVWLFFLGGIATFCIVWGGRMIRSFKAA"
     gene            2750..4510
                     /gene="dnaX"
                     /locus_tag="PKH81_00225"
     CDS             2750..4510
                     /gene="dnaX"
                     /locus_tag="PKH81_00225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNQ96501.1"
                     /translation="MAYEVTATRRRPGQFKDLIGQEFVVATLQKSIEAGKIAHAYLFS
                     GPRGCGKTSSARILARALNCEKGPSAVPCGQCSACLSIARGSNMDVLEIDGASNTSVN
                     DVRQIKDEVQFPPNSCRYKVYIIDEVHMLSTSAFNALLKTIEEPPPYVVFIFATTELH
                     KVPATIKSRCQQFNFRLVPVELLKEALAQAAAESGITADDDALFWVAREATGSVRDAY
                     TLFDQVSAFSDGHLTFAKIEDKLGLVGIDRINHIMSACAQRNVKEALSLLDEILQAGV
                     SVEQCTVDLADYLRGLLLLKGGVTREALLGQSPDRYPGSVLSSWSIPCVERALSIALQ
                     LFRDIRYSVDSRYELELAVSRLAWISDYVSSAELQDAIGRAQALLKGSGGQSVQGKPA
                     APFVEAGLTEASIEQVQVRQASLPTQSSAEEVGRTDEESLVADPFAQLKARMNGKTLV
                     QSDPVPEQSREPDSVSTEDLPVVQAPTVDIAELRDELVRFFEPTKNMLAVALSQTFDW
                     EDDGQTIGLCVSKVFVHKYLEEEQKTIGDALCRYYTKGRTLAIKLVASAAHEEQEDEG
                     IPERVDMVVKMFRGTIVGGA"
     gene            4516..4815
                     /locus_tag="PKH81_00230"
     CDS             4516..4815
                     /locus_tag="PKH81_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNQ96502.1"
                     /translation="MNPFDILKNAKAIQEQVGRLQEEMSGMVVSGSSGGGIVSLTMNG
                     QFEILSLHIDPVAVDGRDIPMLQDLIIAAHTDAQGKIREALKDRIGPLAGGFPGL"
     gene            4812..5399
                     /gene="recR"
                     /locus_tag="PKH81_00235"
     CDS             4812..5399
                     /gene="recR"
                     /locus_tag="PKH81_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNQ96503.1"
                     /translation="MNALDDVIEVFSRLPGIGKKSASRIAYHLLKSDYSVCERLARDI
                     LSLQQKIHECRVCGSYTEDEECRICADSSRDHSLICVVEEPQDVQTIESVREFNGVFH
                     VLGGVIAPLEGIGPDQLRLSSLFSRISAGGVSEVIIATNPTVEGDTTALYIQRALKPF
                     GVTVTRLAFGLPVGGDLEYADRLTLTRSFRGRGVF"
     gene            complement(5396..6832)
                     /locus_tag="PKH81_00240"
     CDS             complement(5396..6832)
                     /locus_tag="PKH81_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96504.1"
                     /translation="MRTLTELQLRIIKEEFPDINLDGDTDVERYFELRSIGRHSDALS
                     LYSSRITKKYPDDVQRALLMRYYRQRDPRYTLLHQDNLVSLAERMVGRIHSIITFMTK
                     DIDSVNLKDAYSVIKLAEGLLSVISNDRWQALSFAERYARYATLLSFKSREMANTAEL
                     LRLYVTETLESVEELKKEREEKIRLQRKKKLLEGSSRKTFDLSAIHFSNQEIARILIP
                     EQITRVEDTVIAYCLKYWNLVFDGAFEKSVFLYSKKFHTRHSDIFQAIKNGISHGWKD
                     EEILNAVLANVVSGYYYSISGDRYLQHTWARYKGSLGMPQATPQPEPSLPIAEKPQRK
                     RTHKKVPLKKSPQTKALPRISIPAPKKQPHKAVAQTAPFSPNSITDIIKKMTGKTYTV
                     YKDLFFQDIRPSIRAVLANAAGKKEGLFSTSKQNQAEELVYGYLFDHYSDPYQNWTNS
                     EERKRIEIIGYPLSSIEPIIERWIKDTR"
     gene            complement(6914..>7499)
                     /locus_tag="PKH81_00245"
     CDS             complement(6914..>7499)
                     /locus_tag="PKH81_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNQ96505.1"
                     /translation="LSVQSSNGIYSAEDRMQIQVEVSQLVAEVDRIASHAQFNGMNML
                     TGRFARETGENTVTGSMWFHIGANMDQRTRVYIGTMTASALGIRNVGDERIMTLENAD
                     SANRAIGTLDAALKTVNKQRADLGAYQNRLEFTMKGLAVGAENLQAAESRIRDTDMAR
                     EMVDFTKNQILTQSGTAMLAQANQQSQSILSLLK"
BASE COUNT         1729 a         1664 c         1962 g         2144 t
ORIGIN      
        1 tcaatggggg ccacaagaag cggatgaatc tttagcccga agctggcgat gtgtgtttga
       61 ccgcgaggag gtaagattgt gatttttgtg gaacctctgg tgcgagtgga aggacttacc
      121 aaaaaattca atcttgaagc gggttttttt gcacgctttg gaaaatttgt tcacgcggtt
      181 aatgaccttt cgtttaccat aggcaagggt gaaacctatg cgcttgttgg agaatcaggt
      241 tgcggcaaga caaccacggc acggcttatt gtacggatgt atgagcgcga tgcaggtaca
      301 attctttttg gtcgggatac tgatgtaggc ctcttgcgcg ggcgcgactt gcgctcgtat
      361 cgagaaaaag ttaagtatgt gtttcaagat cccgcgcgat ctcttaatcc gcgcatgagc
      421 gtgtataatg tgctaacctc tggatacaga tggtcgagcc gatggcccgg cgcgcgtcgg
      481 gcgcgggaag aagctgcatt gatacttgaa gaagttggac ttgctccttc ggatttggat
      541 cgacgccccg cagatttttc gggaggacag cgccagcgaa tatctattgc ccgaagtctt
      601 ttaatgcagc cggagttatt gatttgcgac gaggttgtct ctgcccttga tgtttctatt
      661 caaggacaga tactaaacct tttgcaggat attcgggaaa agcgcggtct gtcgatattg
      721 tttatcgccc atgatcttaa agttgcctcc tatttttgcg atcgtatagg ggttatgtat
      781 cgcggagagt tgatggaaga aggcagggcc caggatttgt atcgcaatca tgggcatccc
      841 tatacgcagc ttttgtttgc gagtgcaacc ggaggaacgg aggaagcccc tggctctgtc
      901 accaccccgc agtggaacag tgcacacggc tgcgcctttg cgcatcgatg cccgctagcg
      961 gataatagct gttatgcaca aaggcccgaa tggaaaaaca ttggggacga tcattttatt
     1021 cgctgtcata agcgataaca cataaggatg tggtaagaga atgagcggag cgcgtaaagc
     1081 tactcggcca acggccgcta ttttatacga ttttgataaa accctgtgca cccgggatat
     1141 gcaggaatac agttttattc ccggccttgg tctttctgca caagaatttt ggagcgaggc
     1201 ctcccgtctt tccgaacagg ggatggatcc aatattagcc tatatgtacc tgatgctcaa
     1261 aaaatcccgg gaagcggagc agccgattcg tcgcaatgat tttgtgtcca tgggaaaaaa
     1321 catcgcattt ttccccgggg ttcttgaatg gtttaataga attagttcat atggaaaaag
     1381 tgcaggcctt gaggtggaac actttatcat ctcttcgggg ttaacagaaa ttatcgaagg
     1441 ctccagcata ggagctcagt ttaataaaat ttatgcctgt gcctttcatt atgatgttaa
     1501 cggtgtggcc gactggccgc ttgtttcggt gaattataca accaagactc agtttctgtt
     1561 caggattaac aagggcgtgc ttgacggtcg tgatggagag ggcttgaacc ggtttgttcc
     1621 ggaagatgag cgacctgttc cattcagaaa tatggtgtat attggcgacg gtatgacgga
     1681 cattccctgc atgaagctgg tcaagacaaa tggcggacat tctatcgcgg tgtatcctaa
     1741 ggatcaaaag gaaaaggcag cgcctttgct tagggataaa cgggttgatt ttattgcgag
     1801 tgccgattat cgcgcagata aagagcttga tcgccttgtt aaaaaaattt tggaccggat
     1861 atcagtggta catatgttaa acgaaagtaa tcgtgcccag aggcaaagtc tgggtaaagt
     1921 gtgaggtata acgagccggg gccagtagac gctgtagggg cttttttaat tgctgtggtg
     1981 tatattcttc tcgctggtat tctggcgcag cttcctgcgc tctctcccct tattatcttt
     2041 tttcctcttg gctttgcctt gttttcgttt gtctatgccg cagccagatc tttttacctt
     2101 ccctccgtat ttccacttgt tcggcccacg ctcaagcaag tagcaggagc taccttgatg
     2161 attgtgggaa ttctctgtgt aattaccttc cttatgatta ctgtccaggc gatttttcct
     2221 tcgttctccc cggatgcgat agatgccgag gaagtactgt ccaagggtct tttatccggg
     2281 attattggag tagccctttt gcctgcggta tccgaggaaa tattgtgccg tggcttgatg
     2341 ctcaaggcct taagggggtc tgttccgcgc ttggtcgcga tagtggtgtc gaatcttctt
     2401 tttgctgttc ttcattttga tatctatcgc atacccttta cctttgtatc gggtttagtg
     2461 attacctggg tcgcctacga aacagcctcg ttggtgttgc cggttatcat gcattttttg
     2521 cataatatga ttttgtttgt tattgcctgg tcgatagatc gatatgtaaa gccagaggcc
     2581 atggcacagg aaggccttga aactcttgcc gcatccgatc caaggctctt gtggttttcc
     2641 ttgcttgtct ggcttttctt tttaggtggt attgccacgt tctgcattgt gtggggcgga
     2701 agaatgatcc ggtctttcaa agcagcttga tttatggtat tctgatccta tggcatatga
     2761 agtaaccgcg acacggcgca ggcccggcca gttcaaggat ttaatcgggc aagagtttgt
     2821 tgtagccacc ctgcaaaaat ctatagaagc cggcaaaatt gcccatgcct atctgttttc
     2881 cgggcctcgc ggatgtggaa aaaccagttc tgcgcgaatc ctagcccgtg cccttaattg
     2941 cgaaaaggga ccttccgcag ttccctgtgg tcagtgctct gcctgtcttt ctattgcgcg
     3001 cggctccaac atggacgtgc tggaaattga cggtgcgtcg aataccagtg tgaacgatgt
     3061 acgtcaaatt aaggacgagg ttcagtttcc ccctaattca tgcaggtata aggtatacat
     3121 cattgacgaa gttcatatgc tttctacaag cgcgtttaac gcgctgctta aaacgataga
     3181 agagccgccg ccctatgttg tttttatctt tgcaaccacg gagcttcaca aggttccggc
     3241 tactataaaa agtcgatgtc aacaatttaa ctttcgtctt gtaccggttg agctgcttaa
     3301 ggaagctctt gcccaggccg cggcagaaag cgggattact gctgacgacg atgccctgtt
     3361 ttgggttgct cgcgaggcca ctggcagtgt ccgcgatgcc tacacccttt ttgatcaggt
     3421 gtctgccttt tccgacgggc accttacctt tgcaaagata gaagacaagc ttggccttgt
     3481 gggtatagat cgaattaacc atatcatgag cgcctgcgca cagcgaaatg ttaaggaagc
     3541 tctttctctt ctggacgaga tactacaggc aggtgtgtct gtagagcaat gtactgtcga
     3601 tttagccgat tatctgaggg gccttctgtt attaaaaggc ggcgtaactc gcgaggcttt
     3661 gcttgggcag agtcccgatc gctaccccgg ctccgttctt tcttcatggt ccatcccctg
     3721 cgtagagcgc gctctttcca tagcgcttca gctattccgc gacattcgct attccgtgga
     3781 tagccgttat gaattggagc ttgctgtttc tcggcttgca tggataagcg attatgtttc
     3841 gtcggcggag ttacaggacg ccatagggcg cgcccaagcc ctgttaaaag gctccggagg
     3901 acaaagtgtt caggggaaac ccgcagcccc ttttgttgag gcgggcctga ccgaagcgtc
     3961 cattgagcag gttcaggtcc gccaagcgtc tcttccaaca caaagcagtg cagaagaggt
     4021 gggccgcact gacgaagaga gcctggttgc cgatcccttt gcccagttaa aagcacgtat
     4081 gaatggaaag actttggtcc aaagcgatcc ggtccctgaa cagtcccggg agcctgattc
     4141 tgtttcgacg gaagacttgc cggttgtaca ggctcctacg gtggacattg ccgagcttcg
     4201 cgatgagctt gttcgctttt ttgagccaac caaaaatatg cttgcggtgg cgctttccca
     4261 aacctttgat tgggaagacg atggacagac gattggcctg tgtgtaagca aggtgtttgt
     4321 tcataaatat cttgaagaag aacaaaaaac catcggtgac gctctttgtc gttattatac
     4381 aaaaggtaga acccttgcca tcaagcttgt tgcttctgcc gcccatgagg aacaggaaga
     4441 cgaaggaatt ccggagcggg tggatatggt agtcaagatg tttcgcggaa caatagttgg
     4501 aggagcataa cgaatatgaa tccctttgat attcttaaga acgcaaaagc aattcaagaa
     4561 caggtaggtc gcttgcaaga agagatgagc ggcatggttg taagcggctc ttcgggcggc
     4621 ggtattgtta gcctgaccat gaatggacag tttgaaattt tgtctctcca catagatcct
     4681 gttgcagtgg atgggcggga tattcccatg ctgcaagatt tgattatcgc tgcccatacg
     4741 gatgcacaag gaaaaatccg cgaagcactt aaagatcgta ttggcccgct cgcgggggga
     4801 ttccctggtt tatgaatgcc cttgatgatg taatcgaggt cttttcgcgc cttcccggta
     4861 ttggaaaaaa gagcgcgagc agaatcgcct atcatctgtt aaaaagtgat tattctgtct
     4921 gcgagcgttt agccagggat attttgtctc tgcaacaaaa aatccatgaa tgtcgtgtat
     4981 gcggctcata caccgaagac gaagagtgcc gaatctgtgc tgattcttca cgggatcact
     5041 ccttgatttg tgttgtagaa gaaccccagg acgtacagac gatagaatct gttcgagagt
     5101 tcaacggcgt ctttcatgtc ttgggtgggg tgattgcgcc gctggagggt atcggccccg
     5161 accaactgcg actttccagt ttgttctccc gcatttctgc agggggtgtt tccgaggtaa
     5221 ttatcgccac aaatcctact gtggaaggcg ataccaccgc cttgtatatc caacgcgctc
     5281 ttaaaccttt tggcgttacg gttaccagac ttgcctttgg cttgccggtt ggaggagatt
     5341 tggaatacgc tgaccgactt acccttaccc gtagtttccg gggacgaggc gtgttctagc
     5401 gggtgtcttt aatccatcgt tcgattattg gttcaatgga tgaaagggga tatccaatta
     5461 tttcgatacg ttttcgctct tcgctgtttg tccaattttg atatgggtcg ctataatgat
     5521 caaaaagata gccatatacc agttcttctg cctggttttg ttttgatgta ctaaaaaggc
     5581 cctctttctt gccagctgcg tttgccaaca ctgcgcggat tgacggacga atatcctgaa
     5641 aaaataaatc tttgtacacg gtgtatgttt ttcccgtcat cttcttgatg atgtcggtaa
     5701 tggaattggg agagaaggga gccgtctgcg caacagcttt atgaggctgt ttttttggag
     5761 cgggaatcga aatacggggc agggcctttg tctgcgggct ctttttaaga gggacttttt
     5821 tgtgagtgcg ttttcgctgt ggcttctctg ctatgggcag agatggttcg ggttgtggtg
     5881 ttgcttgcgg cataccgagc gaccccttgt atctggccca ggtgtgttga aggtagcgat
     5941 ccccggagat actgtaataa taaccggata ctacgttcgc cagcacggcg tttaaaattt
     6001 cttcatcctt ccacccatga cttatgccat tttttatggc ctggaagata tcactgtgtc
     6061 gggtatggaa ttttttgcta tacagaaaaa cagatttttc aaaggctcca tcaaaaacaa
     6121 gattccagta ttttaagcaa tacgcaataa ccgtatcttc tacacgggta atctgttcag
     6181 gaatgagtat gcgcgctatt tcttggttgg aaaaatgaat ggcagacagg tcaaaggttt
     6241 tgcggcttga accttccaat agtttctttt ttcgctgcag gcgaattttt tcttctcgtt
     6301 ccttttttaa ttcctcaaca gattccagtg tctcggtaac gtacaggcgt aaaagctctg
     6361 cggtgtttgc catttctcgc gacttaaaag aaaggagtgt ggcatagcgg gcatatcgtt
     6421 cggcaaaaga aagtgcttgc caacgatcat tggaaataac tgataataat ccttcggcca
     6481 atttaatgac ggaataggca tccttaagat ttaccgagtc tatatccttt gtcataaagg
     6541 tgattatgga atgaatacgt ccaaccatac gctcggcaag ggagacgaga ttgtcctgat
     6601 gtaaaagcgt atatctggga tctcgctggc ggtagtatcg catgagcagg gcccgctgca
     6661 catcgtcggg gtattttttg gttattctgg aagagtacag ggaaagcgca tcggaatggc
     6721 ggccgatgga acgtaactca aagtaacgct ctacatcggt gtctccatcg aggttgatat
     6781 cggggaattc ttctttgatt attcgcagtt gaagctcggt aagcgtgcgc atgacaaccc
     6841 tttatatcaa aaaaaagggt taattcccag tagataccgg aaattaaccc tgtccgtcgt
     6901 tctgtgcggt cgtttacttc aggaggctca agatgctctg cgattgctga tttgcttgcg
     6961 cgagcattgc ggtgccggac tgagtaagga tctggttttt ggtaaagtcg accatttccc
     7021 tcgccatgtc ggtatcgcgg atccgcgatt ctgcggcctg gagattttcg gctccaaccg
     7081 caagtccctt catggtaaat tcgaggcgat tttggtacgc gccgaggtct gccctctgct
     7141 tgtttaccgt tttaagtgct gcatcgagtg taccgatggc acggttcgca ctgtcggcgt
     7201 tttcaagggt cattatcctt tcgtcgccaa cgtttctgat accaagggcc gaggccgtca
     7261 tggtaccaat atagacgcgg gttcgctgat ccatgttagc tccaatatgg aaccacattg
     7321 aaccagttac ggtattctct ccggtttccc gagcgaatct tcctgtaagc atgttcattc
     7381 cgttgaattg ggcatggctt gcgattcggt ctacttcagc aacaagttgt gaaacttcga
     7441 cctggatctg catgcggtct tctgcggaat agataccgtt tgaagactgt acgctcaat
//
LOCUS       DAOQXQ010000007         7013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_11130_length_7013_cov_2.732969, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000007 DAOQXQ010000000
VERSION     DAOQXQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11130_length_7013_cov_2.732969"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKH81_00250"
     CDS             complement(<1..494)
                     /locus_tag="PKH81_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNQ96506.1"
                     /translation="MTAKYSIIVWDWNGTLLDDVAINLHIVNTLLHTRGIPPISSERY
                     KQVFRMPIRDFYHDIGFDMTKEPFEVIAHEYNRLYTAHFDTISLTEGVHDVLCHARDK
                     GIDQYILSASEKTSLAYQVNQKGISPFFQAIVGNDDFSVIGKIDKARLLAKELGNTKN
                     IIFIG"
     gene            complement(494..2023)
                     /locus_tag="PKH81_00255"
     CDS             complement(494..2023)
                     /locus_tag="PKH81_00255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ96507.1"
                     /translation="MIGINSVTSALLFAGALYAGILSVYTFQKMRTHSEGIPLFLLMG
                     ASFFYSAGYGCELFSGDPSWTFAWLRVQYIGLSFMPYLIFTLAMSFTGIYPFKGKISF
                     IFLGVALITFISIQTNPYHHLFYTFIATDTSGPFPTSHLGKGPLYALHMSMFIASCLV
                     SFFLYLKSVITSRGMDKTRQIVLTSTTVFPLAGILLYLLEIVPWHIDPSPLSALLCCN
                     LMVFGIHKTGLLELGPLARTLVFSSMSEGVIVTSKPELIADYNNAILKLFPSLSTDMR
                     GKNLSRAIPGLTDKLPSENDYVDFCHTNTCYEIKNLPVVNTKGKAIGNAYIFRDVTRE
                     REYIESLKKRALIDGVTELYNRLHWEELAVQAIEQSAKNNNECAIMMVDLDHFKDVND
                     SYGHEFGDKILRSVSKRMLSVLRDNDLLGRYGGDEFCLCLPNTGKADCIAFCERLHKD
                     VATLSVSSGSTSRPVSISIGITIIQPGQETTLTKSLSQADQALYRAKRNGRNRTECIE
                     V"
     gene            2137..5964
                     /locus_tag="PKH81_00260"
     CDS             2137..5964
                     /locus_tag="PKH81_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNQ96508.1"
                     /translation="MKTNTFKLIIVSIVACALIVTGLLVIPGFREGPQFTIVTMDGIE
                     QITISQINRLEPLTIRFAEPLQRPAALAQAVSLRPKVQGIWSCKDDRTIEFTPSRPYA
                     ADSQLILSVDTGVLMGGVSGDRGFITRFDVNPQSITVIPGGLVPSDKDANIFVLDGVV
                     SAGIPISQAEARKAVRASLGKNARKQNLDIIWEREGESTTHAFRIESIARRESDELLT
                     LSWKDASRGEQLNEKTWLVPKRDEFRVIEVAQEGPATVKVLFSELLDSRQDIRGFVRI
                     GNEHNVRYSINKNSLTVYGKENWIADQKIEILEGLQSDSGKVLMVPVSSTVSSSWDKP
                     AVRFVTDGVILPTTDGVVLPIETLNLSGVLIEAHKIHGDNVLQFLQVNELDGSRELRR
                     VSDPIWTKSIDFDWNEGMKNRYVPRGLDLSELVKKDPGAILQIKVTFRKRHTKYVCTR
                     GHRDFSSLPEPPDSIGQPEEESEQSYWDYAEEMDYDTRREYRTYRDDPCHPAYYMSEY
                     NSSCVASRTVLVSNLGIMIKKDVDGAFAVSVADIGTTNPIAGAEITFYSYAQGKILTG
                     VTNKDGFFSGKAKSAPYFITAHKGGKTSYLRIDDGTSLSVSHFPIDGVSAEKGVKGFI
                     YGERGVWRPGDDIHLVFVRQDLSGSVPKDFPITFELESPNGSIRSTQVISNSVDGFYR
                     IDTRTDEDDESGSWVARVKAGGQTWTKYLRIEAVVPNRLSINLQTDNAILTQGQNNFT
                     LQSAWLHGAPAPDYKADVWVSFVSAPTVFDGYSDYTFTDPRRGIEENNRLVWEGSLDK
                     KSTANVTLDLDSGRDVPGKLKAMMTTRVFEPSGMFSAEQVAYPFSPYNAYVGIKLPKG
                     DATRGMLLTDVKHRIDLAVVDAEGHPIKNDVPLTVKLYKLEWRWWWEKDALTDASYVS
                     HSYDRVIAEDSIVAKKGSATWTMEVKYPEWGRYLAVVEDPSGGHRAGKIVYIDWPGWA
                     GRGGDSGSGSAAMLPLSSDKNTYTVGNTAEITFAANENARALVTLEKNGRILKQEWVK
                     GSKGTTVWKCPITAEMAPNVYAHVTLLQPHMQTVNSLPIRLYGILPLMVEDPATRLKP
                     MVLAPEEYSSGREARFKVLENNGKPMTYTVAVVDEGLLGLTRFTAPNPWYEFYQKERS
                     SLASWDLYRYVMSAYGGKLETILSVGGGEDMGAGNQAKAQRFKPVVLFLGPFELGKGE
                     TKEHVFKMPNYIGAVRLMVVAGKKGAYGTAEKQVPVREKLMILSTLPERLVVTKRSRF
                     PLPYLTDSQLNRP"
     gene            5991..>7013
                     /locus_tag="PKH81_00265"
     CDS             5991..>7013
                     /locus_tag="PKH81_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96509.1"
                     /translation="MVVNESLDVVIDAGSDKTVTLPVSTTVQGKAVVTVAATAIDGSV
                     TATNTTEIQVVSRGSPTVTTRSFYLKAGESKREFVPSPGESGSKAMSIEVSKMPVLDI
                     QSRLSYLLTYPHGCIEQITSGGFPQLYIPSMIDMSPEETDTIKRNVLSVIERYSGYQA
                     FDGGFSYWPSGGESNPWGSCYAGHFMLEAKKAGYEIPDSLYAPWLKYQTEQAKAFNSS
                     GDRDVEVQAYRLYTLALSGNADIGSMNRLRQQNTASVPVKWLLAGAYYLSGQRSQAAE
                     LVRGLAVELERYRDTGSNWGSTTRDQALVLAMMNVLGETNRSIGTLAKVTEALSSSSW
                     YSTQETA"
BASE COUNT         1913 a         1479 c         1748 g         1873 t
ORIGIN      
        1 ccaataaaga taatgttctt tgtattacca agctctttgg caagaagcct ggccttatca
       61 attttcccta ttacggaaaa atcgtcgttc cctacaatcg cctgaaagaa gggactaata
      121 cctttttgat taacttgata cgcaagactc gttttttctg aagcagagag tatgtattgg
      181 tcaatgcctt tatcacgagc atggcataaa acatcatgta cgccttctgt aagtgatatg
      241 gtgtcgaagt gtgccgtata taacctgtta tactcgtgcg caattacctc gaaaggttct
      301 tttgtcatat caaatccaat atcatggtaa aagtccctga tgggcatgcg gaatacttgc
      361 ttgtatcgct cagagctaat tgggggtatc ccccgcgtat gcaaaagagt gtttactatg
      421 tgcaggttaa tcgctacatc atcaagaagg gttccgttcc agtcccatac gataatagaa
      481 tacttggctg tcattatact tcaatacact ccgttctgtt tcttccgttg cgtttggctc
      541 tatatagggc ttgatcggcc tgggacaagc tttttgtgag cgtggtctct tgccctggct
      601 gtatgattgt tatgccaatg ctgatagaaa ctggcctcga tgtgcttccg gagcttactg
      661 aaagagtggc aacgtcttta tgaaggcgtt cacaaaaagc aatacaatcg gcctttcccg
      721 tgttgggaag gcacaggcaa aactcatcac ctccatatct tcccaacaag tcgttatccc
      781 gtagtacgga gagcattcgc ttggaaactg atcgcaaaat tttatctcca aactcatgac
      841 cgtaggagtc gtttacgtcc ttgaagtgat ctagatcaac catcataatg gcgcactcat
      901 tgttattctt ggcagattgt tcaattgcct gcacggcaag ctcttcccaa tgaagtctgt
      961 tatagagttc ggttactccg tcgataagcg ctcgtttctt gaggctttca atgtattctc
     1021 gctctctcgt tacatctcta aagatatacg cgttaccaat agccttaccc ttggtattta
     1081 caacaggcag gtttttgatt tcgtagcacg tgtttgtatg acaaaaatcg acatagtcgt
     1141 tttctgaggg tagtttatct gttaatccgg gtattgccct ggacagattt tttccacgca
     1201 tatcggtgga aagggatgga aagagcttga ggattgcatt gttgtagtcc gcgattaatt
     1261 ctggcttgct tgttacaata acgccttcgg acatggacga gaacaccagg gttctagcca
     1321 gtggtccaag ttcaagaagc cctgttttat gtatcccaaa taccatcaag ttgcaacaca
     1381 acagggcgga gagggggctt ggatctatgt gccagggaac aatttccaga agatataaca
     1441 agataccggc aagagggaat accgttgtgg aagtaaggac aatctgtctt gttttatcca
     1501 tgccccgcga agtgataaca ctcttaagat ataaaaaaaa cgaaaccagg catgatgcga
     1561 taaacatgga catgtgaagc gcatataagg gaccctttcc cagatgggaa gttgggaagg
     1621 ggccggaggt gtccgttgca atgaaagtat aaaacaggtg atgatatgga tttgtttgaa
     1681 ttgaaataaa ggtaataaga gcaacgccaa gaaatataaa agaaattttc cccttgaacg
     1741 gatatatgcc ggtaaagctc atcgcgagag taaaaattaa atatggcata aaggacagtc
     1801 caatgtattg cactctaagc catgcaaagg tccaggaagg gtcgcctgaa aataattcac
     1861 atccataacc ggcagaataa aaaaatgatg cacccattaa caggaatagt ggaataccct
     1921 cgctgtgtgt acgcattttt tggaaggtgt atacagacag aattcccgca tatagtgcgc
     1981 ccgcgaagag gagagctgat gttacactgt taatgccgat catagggtct tctcctcaaa
     2041 taaaaggtag tgtagcacat tatccagttt gcgaaaatga aaaagagagc ggcttaccgt
     2101 tttgttccat caggaattat gtgatatact ggtttaatga aaacaaatac gttcaaactc
     2161 atcatcgttt cgattgtcgc gtgtgcgctg atcgtcaccg gccttttggt tattcctggt
     2221 tttcgggaag gtccgcaatt taccatcgta acaatggatg gcattgaaca aataacaata
     2281 tcccaaatta atcggctgga accgcttact atcaggttcg ccgaaccatt gcagcgtcct
     2341 gctgctcttg cacaggctgt ttcccttagg ccgaaagtac aagggatctg gtcgtgtaaa
     2401 gatgatcgaa ctatagagtt tacgccttct cgcccatatg cggccgactc gcaattgatc
     2461 ttgtctgttg acactggtgt attgatgggt ggtgtatccg gcgacagggg ttttattacc
     2521 cgctttgatg ttaatcctca atcaataacc gtaattcccg gaggtcttgt cccttcggac
     2581 aaggatgcga atatatttgt gctcgacggt gttgtgtccg ccggaatacc gatttctcag
     2641 gcagaggccc gaaaagcggt acgcgcgagc cttggaaaaa atgcaagaaa acagaacctg
     2701 gacattattt gggagcgcga aggggaatca acaacccatg ccttccgtat tgaatcaatt
     2761 gcccgtaggg aaagcgatga gctactcacg ctaagctgga aggacgcatc ccgtggagaa
     2821 cagcttaacg agaaaacctg gcttgttcca aagagggatg aattcagggt tatagaagtc
     2881 gcccaagagg gacctgctac ggtaaaggtt ttattctctg aattgcttga ttctcgccaa
     2941 gatattagag gctttgtccg catcggtaat gagcacaatg tgcgctattc aataaacaag
     3001 aactcattaa cggtgtatgg taaagagaat tggattgccg atcagaaaat cgagatatta
     3061 gagggattgc agagcgacag cggaaaggtg ctgatggttc ccgtatcctc aacagtcagt
     3121 tcctcatggg ataagccagc cgtacgattt gtaaccgacg gcgtaattct tcccaccaca
     3181 gacggagttg tgctaccgat agagacgctt aatctttccg gggttcttat agaggctcac
     3241 aagattcatg gcgataacgt acttcagttt ttacaagtga acgaacttga cggctcgcgt
     3301 gaattgcgtc gtgtgtcgga tccgatatgg acaaagtcaa ttgattttga ctggaacgag
     3361 ggcatgaaga accgttatgt accccgaggc cttgatttaa gcgagcttgt caagaaagat
     3421 cctggggcca tattgcaaat aaaggtgacc ttccgtaaac gacataccaa gtacgtatgt
     3481 accagagggc atcgtgattt ctcttcattg cccgagcctc ccgacagcat tggacagcca
     3541 gaagaagaaa gcgagcaaag ttattgggat tatgcagagg aaatggatta cgatacgcgc
     3601 agggaatatc gtacctatcg ggacgatccc tgccaccctg catattatat gagcgaatat
     3661 aacagctcat gtgttgcttc gcgaactgtc cttgtttcca atttggggat aatgataaag
     3721 aaggacgtgg acggggcttt cgctgtttct gttgcggata tcggaactac caatccaata
     3781 gctggagctg agattacctt ttatagttac gcacagggaa aaatccttac cggggtaact
     3841 aataaagacg gattttttag cgggaaagca aagtcagctc cctattttat cactgcgcac
     3901 aaaggtggaa aaacttcgta tttacgtatc gatgatggaa cttcattaag tgttagtcat
     3961 tttccgatag atggagtgtc cgcagaaaag ggcgttaagg gctttatcta tggagagcgg
     4021 ggagtatggc ggccgggaga tgacattcat ctggtgttcg tcaggcaaga tctttcgggt
     4081 tcggttccca aggattttcc gattaccttc gagcttgaaa gtccaaatgg tagtattcgc
     4141 tcaacacagg ttatatctaa ctccgttgat ggtttttatc gaattgacac acgaactgac
     4201 gaagatgatg aatcgggttc atgggttgcg cgtgttaagg caggtggaca aacctggact
     4261 aagtacctgc ggattgaggc tgtagtgcca aatcggcttt ccattaatct tcaaactgac
     4321 aatgctatat taacacaggg ccaaaataac tttaccctac aaagcgcctg gcttcatggg
     4381 gctcctgcac cagactataa ggctgatgtg tgggtttcct ttgtatccgc tccaacggtg
     4441 ttcgatggat attcagatta tacttttact gatcctagac gaggtatcga agagaacaat
     4501 cggcttgtgt gggaaggctc gcttgataaa aaatctacag ccaatgtcac ccttgatctt
     4561 gattcaggac gcgatgttcc gggaaaacta aaggcaatga tgacgacgag ggtgtttgaa
     4621 ccttcgggga tgttttccgc cgagcaagtt gcatatcctt tctctcccta caatgcatat
     4681 gtcggcatta aactgccaaa gggagacgca actcggggca tgcttcttac tgatgttaag
     4741 catcgtattg atcttgctgt tgtcgatgcg gaaggtcatc cgattaaaaa tgatgttccc
     4801 ttaacggtca agctctataa actcgaatgg cgctggtggt gggagaaaga tgcgttaact
     4861 gatgcctcct atgtatccca ttcgtatgat cgtgtgattg ccgaggattc aattgtagca
     4921 aaaaagggaa gtgctacctg gacaatggaa gttaagtatc ctgaatgggg acgttatctt
     4981 gccgtggtag aagatccctc cggtggacat cgggcaggaa aaattgtgta tattgactgg
     5041 ccaggatggg ctggtcgtgg tggagattct ggttctggaa gtgcggcgat gttaccattg
     5101 tcttcggaca aaaacactta taccgtaggt aacacggcag agataacctt tgccgcgaat
     5161 gaaaacgccc gggccctggt tacactggag aaaaacggaa ggattctaaa gcaggaatgg
     5221 gttaaggggt caaaggggac tactgtttgg aaatgcccca ttacagcaga gatggctcca
     5281 aacgtatatg cgcatgttac gctcttgcaa cctcatatgc aaacggtaaa tagtctgcca
     5341 atcaggcttt acgggatact gccgcttatg gtagaggatc cggccacccg actgaagccg
     5401 atggttcttg cgcccgagga atattcatcc ggaagagaag caagattcaa ggttttggaa
     5461 aataacggaa agccgatgac gtatactgtc gcggtagtgg acgaaggtct tcttggtctt
     5521 acccgtttta ctgccccgaa tccatggtac gagttttatc aaaaagagcg atcctctttg
     5581 gcaagttggg atttgtaccg ctatgttatg agcgcgtatg gtggaaaact tgaaaccatt
     5641 ctctccgttg gtggaggcga agacatgggt gcgggtaacc aggcgaaggc acagcgattt
     5701 aagcccgttg tcctttttct tgggccattt gagcttggta aaggcgagac caaggaacat
     5761 gtattcaaga tgccgaatta tattggtgcc gtaagactaa tggtagtcgc gggaaagaaa
     5821 ggtgcctatg gcacggcaga aaagcaggtt ccggttcgtg aaaaactaat gattctttcc
     5881 acactgccag aacgcttggt agtaacgaaa agatcaagat tcccattacc atatttaacg
     5941 gacagccaac taaatcggcc ctaaaagttt ctttaaagtc gactggagcc ttggttgtaa
     6001 atgaaagcct tgatgtggtc attgacgcag gaagtgacaa gacggtaaca cttccggttt
     6061 ccaccacagt acagggaaag gctgttgtta ccgtagccgc caccgcgatt gatggatcgg
     6121 tcaccgcgac caatacaacg gagattcagg ttgtatcgcg gggtagtccg actgttacca
     6181 cccggtcttt ctacttaaaa gccggtgaat caaaacgcga gtttgttccg agccccggtg
     6241 agtctggcag taaggcaatg agtattgaag tatctaaaat gcccgtcctg gatatccagt
     6301 ctcgtctttc gtatctgctt acctatcctc atggatgtat agaacagata acatctggtg
     6361 gtttccctca gctgtatata ccgtccatga tcgatatgtc tccagaagaa actgatacca
     6421 taaaaaggaa tgtattgtct gttattgagc gttactcagg atatcaggcg tttgacggag
     6481 ggttctctta ctggccatcg ggcggagaaa gtaatccttg gggttcctgt tacgcagggc
     6541 actttatgct ggaagcaaag aaagcaggat atgaaattcc cgattcgtta tatgcaccgt
     6601 ggttgaaata tcagacagaa caagccaagg cgttcaattc aagtggtgat agggatgttg
     6661 aggtacaggc gtatcggttg tatacacttg ccctgtctgg gaacgctgat attggttcga
     6721 tgaatcggtt gcggcaacaa aacactgctt ctgtcccggt taagtggctg ttagccggcg
     6781 cgtattacct ttccggacaa cgctcacagg ctgctgaact tgtgcgtggt ctggcagttg
     6841 aacttgaacg ttaccgcgac actggtagta actggggatc cacaactcgt gatcaagcgc
     6901 ttgttcttgc catgatgaat gtactcggag aaacaaatcg ctcaattgga acccttgcca
     6961 aggtaaccga ggccttatct tcttcgagct ggtactccac acaagaaacg gcc
//
LOCUS       DAOQXQ010000008         6820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_11532_length_6820_cov_3.172653, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000008 DAOQXQ010000000
VERSION     DAOQXQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11532_length_6820_cov_3.172653"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKH81_00270"
     CDS             complement(<1..1045)
                     /locus_tag="PKH81_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ96510.1"
                     /translation="MMYLRMSLRNIKKNIRKNVLTMTMISFGIVALYVYTGSNTQMFR
                     QFRESVIRDQYGHFQLYSKGYLDEESTEPYQHMITDYHTVAQSLMEDDDIDFLAPRLH
                     FSGVGSSDERSILLKGFGGDPEAEKRMEYASVSSGVFINPDTRYQAVVGENALAKSAS
                     NLGEYLTVLATMNGGGVSAMDFGITGTKQSYGETDRLNDLFILADLASVQELLDLKDS
                     VDTIIVHLASGVNASKKEKDIQQFCEKWNLEYRRWDDLAVFYERSRQVFSMNEQILTA
                     IILVISIFIIINTLYMAYMARMREIGTMRAMGTTKAQITRIILGESMFLSLCGCLSGL
                     VIALLLSFFVYLSG"
     gene            complement(1042..1701)
                     /locus_tag="PKH81_00275"
     CDS             complement(1042..1701)
                     /locus_tag="PKH81_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ96511.1"
                     /translation="MLISVLNVTKTYMMDKVMIPALTDVSLEIDSGDFICLMGPSGAG
                     KSTLLHVLGCIETPTSGDVCIRGENTRAMNDASLSRFRNRHIGFIFQSFNLVSVLNVR
                     ENVEYPLAIRGGKIDRSRVESVIESVGLVDYMNHRPDELSGGQRQRVAIARALVTNPD
                     IILADEPTANLDSKTGEKIIDLLVHLNRDCGTTFIFSTHNEALSHYAHRTIGILDGRL
                     A"
     gene            1907..2395
                     /locus_tag="PKH81_00280"
     CDS             1907..2395
                     /locus_tag="PKH81_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ96512.1"
                     /translation="MNTIKAEKAEPAEVHLVEKGLPIPENSLCLVFDAEHLGDLLRFI
                     NKIAPNRQSPPFLIGRKHETFQPLLLSDILFFRSCGNILYAHTEQQAYEIKHKLFEME
                     KLIACENFIRVNKSNIINVLKIKEIIPWFGGRILLRFTASEERIEVSRNYVKDFKQFL
                     NM"
     gene            2406..2813
                     /locus_tag="PKH81_00285"
     CDS             2406..2813
                     /locus_tag="PKH81_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96513.1"
                     /translation="MDTIKDILIVLIISALVGFGVSGGFALVNNRPLLQTLIAGLLSG
                     SIIGLLSRICFMFVYFNFRNKPALAFMVVIAIIGTATTLISLYFDIRFPFPAIPLIAA
                     SALCGTIVTAIMFHNYNTINARLKQKKEKLDQR"
     gene            complement(2827..3366)
                     /locus_tag="PKH81_00290"
     CDS             complement(2827..3366)
                     /locus_tag="PKH81_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNQ96514.1"
                     /translation="MNTTASKAILVSQLSYRNRNAVIGQIVFVITALSLPLVTHRLGL
                     DYRSAQPMHWMIFFAGLTYGPISGMAVGFLVPVLSHIFTGMPGPMMLVLMIPELMCYG
                     LVAGFLKKRITAFGAMLVASLSGKAVYLGIACMTAKASGSLFSAMSNTWEKSIPAIIV
                     MIVLLPVMSGLYIRVCKRD"
     gene            3468..4418
                     /locus_tag="PKH81_00295"
     CDS             3468..4418
                     /locus_tag="PKH81_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96515.1"
                     /translation="MTRLTAFFFLIALVASTSYAHVANRGVCSRNGEICLINVETGEY
                     TSLTSGHNDMKPSWSMENDQIVFFRVTKMTDRVQTWKTTICIVNADGSNFTQLTSGEY
                     TDYNPTWTRNGKHEILYSRYFSSRGRSVIHRIRFSGETIEDEIISDPKQKEYALSTLK
                     DGRILITSNRSIFTSIHWLLTPATSDTEAVYEPVNFNFKFRSIMDRATFSPDETNIAF
                     EYKAGYGSFSYEDKQITIADFDRQSITISNKRHISPRARQAMTLYPRWLADGKSLVYH
                     SNREGPARLYHYNLETEQTTTVPGDRKIPYEFFCGERSPK"
     gene            4442..5962
                     /locus_tag="PKH81_00300"
     CDS             4442..5962
                     /locus_tag="PKH81_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ96516.1"
                     /translation="MKRYRYYIALFITICSVIYCGAGKYVRLAGNATTNKDEISSVGF
                     TCKDTFDPLSSEAICEMVDEVVVQILGPSKWKTLCKTGDRVVIKVNLVGPHRGKKGEK
                     GVAIITDSRVVQAVAQGVRNAIGPQGDIIVTDTLFYNHENPSLPKEPTSFYNATGYDK
                     DGDGILDGSSGARLVNAESYTENRRFLTIVHTSVLGKTSIWLPDFMRRPQDASKTGEY
                     ADVIIYLPVFKSHGFTGITGALKLGYGLRSESSLPGDTGRYKHSGYGWGTGNKQLLLD
                     YLCAQFKTRPCDFVLLDALTANRKGPLNLSEMDIERGTDWIRTNALYASKDPVALDTV
                     SSLFAGYDPESIALLANAAADGLGTNEPERIRINGATRFTNHRAAIAAQYPPKKSFSG
                     KLKYGNTPGSWPLQDGWGGAKIKIDLEPPIIETVSFKESTTDSIQVVWKATDKSDIIR
                     VDLFCNDITIESTRENTSTGSFNFVPDNKNSYCLVFWDSSLNATERLFYPVIKEKP"
     gene            5959..>6820
                     /locus_tag="PKH81_00305"
     CDS             5959..>6820
                     /locus_tag="PKH81_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ96517.1"
                     /translation="MSADIIIKGVSLTLRQSAMLITDIIPWIVAGALAGACIRKIKRI
                     PFEEKIPYLNSVLAIPAASFSGAASPLCTLGSLPIVTAFLARGLHQGAALAFLASSSI
                     VTPQIALMTAGFLGPRIACMQVAGGFFAGLSAGVLLSLAERTGIQIFRPQDTIHLEEK
                     QLTTRNFIGHVAAQLEYSLFWLVVGVIVSQAAVVISDMSGMSSALSNAASSSKQGSSP
                     GGLFAFFGALLSAPLYSCGGAVLPILAALRAYGIHESFFLAFLICGPATRLRSAAAVG
                     KILTPRALAYY"
BASE COUNT         1988 a         1588 c         1447 g         1797 t
ORIGIN      
        1 cgcccgaaag atacacaaaa aatgacagta aaagagcaat cacaagcccc gaaaggcaac
       61 cgcaaagaga gagaaacata ctttcaccaa gtatgattcg ggttatctgt gccttggttg
      121 tgcccattgc acgcatcgta ccgatttcgc gcattctggc catgtaggcc atatacagtg
      181 tattgataat gataaaaata gaaattacca gaataatcgc agtaaggatt tgctcattca
      241 tggaaaatac ctgacgactc cgttcataaa aaacagcgag gtcatcccag cgtcgatact
      301 caagattcca tttctcacag aattgttgta tgtccttttc ttttttactt gcgtttactc
      361 cggaggctag atgcacaata atggtgtcta cagaatcttt cagatcaaga agctcctgca
      421 cggaggccaa atctgcaagt ataaagagat cgtttaaccg gtcggtttca ccataacttt
      481 gttttgtccc ggtaatgcca aagtccatag cagacactcc gccgccattc attgttgcaa
      541 ggactgtcaa gtattctcca aggttcgaag cacttttagc cagcgcgttt tccccgacca
      601 ctgcctgata ccttgtatct ggattaataa aaacaccgga actaaccgaa gcatattcca
      661 tgcgtttttc tgcttcagga tcacctccaa accctttgag taagatactt ctctcatcac
      721 ttgatccaac accggaaaaa tgaagccggg gggcaagaaa atcaatatcg tcatcttcca
      781 taagtgattg ggcaactgta tggtagtccg taatcatatg ctggtatggc tcggttgact
      841 cttcgtcaag ataccccttg gaatataatt gaaagtgccc gtattgatcg cgaataacgc
      901 tttccctgaa ttggcggaac atttgggtat ttgatccggt gtagacatac aaggccacaa
      961 taccaaacga tatcatcgtc atggtaagaa cgtttttacg gatgtttttt ttgatgtttc
     1021 tcaagctcat acgtagatac atcatgcgag tcgtccatcc aatattccaa tggtacggtg
     1081 ggcatagtgc gaaagagctt cgttatgcgt agaaaaaata aaggtagtgc cacaatcgcg
     1141 attaagatgt accagtagat caattatctt ttcacccgtt ttggaatcaa gattggcggt
     1201 tggctcatct gccaaaataa tatcaggatt ggtaactaaa gccctggcta tggcaacacg
     1261 ttggcgttga ccgcccgata gctcgtctgg gcgatgattc atataatcta caagaccgac
     1321 agactcaatt actgactcaa ctcttgacct gtctatcttg cctccacgaa ttgcaagggg
     1381 gtattctaca ttttctcgaa cattcagcac cgacaccaga ttaaagctct gaaatataaa
     1441 gccaatatga cggttccgaa accgggacaa ggatgcatca ttcatcgccc tggtgttttc
     1501 gccgcggata cacacatcac cagaggtggg agtttctata catcccaaaa catgaagtag
     1561 agttgacttc cctgctccac ttggccccat cagacagata aaatcgcctg aatctatttc
     1621 cagcgagaca tcagtcaaag cgggtatcat taccttgtcc atcatgtagg tcttggttac
     1681 attgagtaca ctgatcaaca ttggcagcct ccagaatgta tagtatcccc acacattaag
     1741 gctgtcgcgc gtttaatgac aacagaacat atctgaatga caacaggaca aaatgcacat
     1801 gcaacatgaa ttgacagatt aacagcggca agcgtactat tacctcatca tgtttatgta
     1861 ccgaatatct gcatcaccgg aaacaaaagc actcatcaag gagctgatga ataccataaa
     1921 ggcggaaaaa gctgagccgg ccgaggtgca tcttgttgaa aagggcttgc cgattccaga
     1981 gaactcactc tgcctggtat ttgacgccga acatcttggc gacctcctgc gctttattaa
     2041 caagatagca ccaaatcgtc agagcccgcc ctttctgatc ggccgcaaac atgaaacatt
     2101 tcaacccctt cttctttcag atattttgtt tttcaggtca tgcgggaata tcctgtacgc
     2161 tcacaccgaa cagcaggcct acgaaattaa acacaagcta tttgagatgg aaaaactaat
     2221 cgcctgcgag aactttatca gggtaaataa gtcaaacatt ataaatgttc ttaaaattaa
     2281 ggaaattatc ccctggtttg gaggacgcat tcttcttcgc tttaccgcaa gcgaggagcg
     2341 aatagaagtt tccagaaatt atgtaaaaga ctttaagcaa tttttgaaca tgtaggagtg
     2401 ctcaaatgga tactattaag gatattctga tcgtactgat tatttccgcg ctggtaggtt
     2461 ttggagttag cggaggcttt gctcttgtta acaacaggcc tttactacaa acgctgattg
     2521 ccggtctttt atcaggttcg attattggtt tgttaagccg gatttgtttt atgtttgtat
     2581 acttcaattt tagaaacaag ccggcccttg cttttatggt ggtcattgca attatcggaa
     2641 cagcaacgac gcttatatcc ctttactttg atattcgctt tccgtttccc gccattccgc
     2701 tcattgctgc ttcagcacta tgtggtacga tcgttaccgc tattatgttt cacaactaca
     2761 acactataaa cgcacggctt aagcaaaaaa aagaaaaact cgatcaacgt taaatggctt
     2821 acaaacttaa tcgcgtttac atactcttat atagaggcct gacataacag gaagtaaaac
     2881 aatcatgaca ataattgccg ggatagactt ttcccatgta tttgacattg ctgaaaataa
     2941 agaacctgac gcctttgccg tcatacatgc aatgccaaga tagactgcct tacctgaaag
     3001 agacgcgact agcattgctc caaaggcagt gattctcttc tttaaaaaac cagccactaa
     3061 tccataacac atcaattccg gtatcataag cacaagcatc attgggcctg gcataccagt
     3121 gaatatatgt gaaagtacgg gaacaaggaa accaacagcc ataccggaaa tgggaccata
     3181 cgttaatcct gcaaaaaaga tcatccaatg catcggctgc gcactgcgat aatcaagtcc
     3241 caatcgatgt gttacaagag gtaatgagag cgcggtaata acaaatacaa tctgtccaat
     3301 aacagcattt ctgttccgat acgaaagttg gctaaccaga attgccttcg acgctgtagt
     3361 gttcaaaaat tacacataca cccaaaagta accgaaagtt accctttgca aaatccacta
     3421 ctcctttaat gatgtgagta acaattactt ctgcaaggag agagtcgatg acccgactaa
     3481 ccgcattctt ttttttgatc gctctggtag caagcacttc ctacgcgcac gttgcaaatc
     3541 gcggagtgtg ttcccgtaat ggtgaaattt gtctaatcaa tgtagaaacc ggcgagtaca
     3601 cctcgcttac atcaggtcat aacgacatga aaccatcctg gtctatggaa aacgatcaaa
     3661 tcgtattttt cagggtaact aaaatgactg atcgagtcca aacatggaaa acaaccattt
     3721 gcattgttaa tgcggacggc agcaacttta cacaactcac cagcggcgag tataccgact
     3781 acaatcctac atggacgcga aatgggaaac atgaaatctt atattcacgt tatttctctt
     3841 caagaggacg atccgttatc caccgcattc gtttctccgg cgagacaatt gaagacgaga
     3901 ttatcagtga tccaaaacaa aaagaatacg ccttaagcac actcaaagac ggacgcattt
     3961 tgattacttc caatcgatca atatttacca gtattcattg gttacttacc ccagcaacaa
     4021 gcgatacgga ggcagtatac gaaccagtga acttcaattt caaatttcgc tcgattatgg
     4081 atcgtgcaac ctttagccct gatgaaacta acattgcttt tgagtataag gcgggctatg
     4141 gctcttttag ctatgaggac aagcaaataa cgatagccga ttttgaccgc cagtctatta
     4201 ctatttcaaa caaacgccat attagccctc gggcaagaca ggccatgacc ttgtatcccc
     4261 gatggctcgc cgacggcaag tctcttgtat accattccaa cagagaaggc ccggcaagac
     4321 tctatcatta caatctggaa acagagcaaa caaccactgt tcccggagac cggaaaattc
     4381 catatgagtt tttttgcgga gaaagaagtc ctaaataatg gtcaaccata aggaaacaca
     4441 catgaagcga tatcgttatt acattgcctt atttattacc atttgctcgg ttatctactg
     4501 tggagcggga aagtatgttc gtctggctgg aaacgccacc acaaacaaag acgagattag
     4561 ttctgtagga ttcacctgca aggacacctt tgatccatta agttcggaag caatatgtga
     4621 gatggtggat gaagttgtag tacaaatcct tggcccaagt aaatggaaaa ctctgtgtaa
     4681 aacgggagac cgtgttgtca taaaggtaaa tctggttgga ccacaccgag gcaagaaagg
     4741 agaaaaagga gtcgcgatca ttaccgactc ccgggtggtg caggctgttg cgcagggagt
     4801 gcggaatgca ataggtccac aaggggacat catcgtaacg gacaccctat tctataacca
     4861 cgagaaccct tcccttccga aagaaccgac tagcttttat aatgccaccg gctatgataa
     4921 ggacggagac ggtattttgg atggatcttc cggcgcgcga cttgttaacg cagagtcata
     4981 tacagaaaat cgcagattcc tcaccattgt tcatacatct gttcttggca aaacttcgat
     5041 ttggcttccg gattttatgc gtcgtccaca ggacgcatca aaaacaggcg aatatgccga
     5101 tgttataatt tatctgccag tattcaagag ccatggattt accggtatca cgggtgccct
     5161 taaactcggt tatggcctac gatcagaaag ttccctgccg ggcgatacag gccgatacaa
     5221 acacagcggg tacggttggg gaacgggaaa caaacagtta ttactcgact acctgtgcgc
     5281 gcagttcaaa acacgaccct gcgactttgt tctgcttgat gccttaacag caaatcgcaa
     5341 aggtccgctt aacctttccg aaatggatat cgaacgaggt accgattgga tcaggactaa
     5401 cgcattgtat gcttcaaaag atcctgttgc acttgacact gtaagctcgc tttttgccgg
     5461 ttacgatcct gaaagcattg cattactggc aaacgcagca gcagacggac ttggaacgaa
     5521 cgagcctgag cggatcagaa ttaacggcgc cacccgcttt actaaccacc gcgctgctat
     5581 tgccgcacag tatccgccca aaaaatcatt ttctggtaag ctcaagtatg ggaatacccc
     5641 tgggtcctgg ccgcttcaag atggatgggg tggggcaaaa attaaaattg atcttgaacc
     5701 ccctattatt gaaacagtgt cgttcaagga atcaactacg gactcaatac aggtagtttg
     5761 gaaggcaaca gataaaagcg atatcatacg tgtagatctt ttttgcaatg atataacgat
     5821 agaaagtaca agagaaaata caagcacagg ttctttcaat tttgttccag acaacaaaaa
     5881 ctcttattgc ctggtgtttt gggacagctc gttaaacgcg accgagcgtc ttttttatcc
     5941 tgttatcaag gaaaaaccat gagtgcagat atcattataa aaggtgtttc actcacatta
     6001 cgacaatcag ccatgctcat taccgacatc atcccctgga tagtcgctgg cgccctggcg
     6061 ggtgcttgta tccggaagat aaaaagaatt ccctttgaag aaaagatccc gtatcttaac
     6121 tctgttctgg caattccggc agcatccttt tccggcgccg cctcgccctt atgcaccctg
     6181 ggaagtttac caatagtaac ggcttttctt gcgcggggcc tccatcaggg cgcggcatta
     6241 gcttttttag catcgtcttc tattgttacc ccgcagattg ccttgatgac ggccggtttc
     6301 ctcgggcccc ggatagcatg tatgcaagtg gcaggaggtt tttttgcagg tctaagcgca
     6361 ggtgtattac tctctcttgc agaaagaacg ggcatacaga tattccgtcc gcaggataca
     6421 atccacctgg aagaaaagca gcttacaaca agaaatttta tagggcatgt agccgcccaa
     6481 ctggaataca gtcttttctg gctcgtagtc ggtgtgattg tatcacaagc agcagtggta
     6541 atctctgaca tgagcgggat gagcagcgca ttaagtaatg ccgcttcatc gagcaagcag
     6601 ggaagctcac cagggggtct ttttgctttc tttggagcac ttctatccgc tccgttgtac
     6661 tcttgcgggg gagccgttct tccgatactc gcagcgttac gagcatatgg aattcacgaa
     6721 tccttttttc tcgcatttct aatctgtgga ccggctacgc gtcttcgttc tgccgcggca
     6781 gttggaaaaa tacttacacc tcgcgcactt gcgtattatc
//
LOCUS       DAOQXQ010000009         6746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_11688_length_6746_cov_2.760574, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000009 DAOQXQ010000000
VERSION     DAOQXQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11688_length_6746_cov_2.760574"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1326
                     /locus_tag="PKH81_00310"
     CDS             25..1326
                     /locus_tag="PKH81_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HNQ96518.1"
                     /translation="MKAHRITLAWCKLLWQTRLGVILQSVLIGITTGFVVLAFRLALS
                     HGEHARSRVYTFLSVHSPLWTLIWIAVIVALGLLLGLLSKLRPMIKGSGIPQIKGELM
                     GKLSHRWLTELPLKFISAVLAIGAGLSLGREGPSVQMGGYIGKAISRLGNRSPAELRF
                     LMISGAAAGLSAAFNAPLAGVLFALEELQKHFSPLMLLCAIAASAMGDFVSSHFFGLA
                     PSFSFPAITPLSLNHFPWLIILGLLSAFCGDLFKQSLYLSQSLYARLGIPPILRPVLP
                     LLVSIPLGFTLAQTIGGGHELVEQLALAQYSVLSIALFFLVKLVFSGLSYGAGTAGGI
                     FLPLLVCGALVGTGFGTVLHTAGIITNIEIVNFLILGMAAFFTAVVRAPVTGTVLILE
                     MSGNLSHLGGLVIVCLIAQLACDLMRSRPVYDVMLYRLTRS"
     gene            complement(1368..1958)
                     /locus_tag="PKH81_00315"
     CDS             complement(1368..1958)
                     /locus_tag="PKH81_00315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ96519.1"
                     /translation="MIYITRHGESTANVEHVFSNRGLKHPLTDKGKKQSENLALLLQG
                     VRITSVYASPILRAIETASIVCSQLHIPRFEVNSLLREYDVGFLEGLSDAQSWDLYFK
                     NERQWISTSNRDSSLFNGETFNQVQQRFRQFIDYLFSSYSPADHNVLIVTHGGLLKIG
                     LPAVVSNQNINEIVEKGIGNCQLLRCQKSGSLLHIG"
     gene            complement(1921..4281)
                     /locus_tag="PKH81_00320"
     CDS             complement(1921..4281)
                     /locus_tag="PKH81_00320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ96520.1"
                     /translation="MNTPKFAKIKDRSSFIITFVLIAIAAIWAIAAVAGISQRNSELA
                     QTEDELVRLNQLVAEQTGSLFREVKGQLILLETWLAANPDKDPRTDKDFIDLVTVIRQ
                     NNRIPIDIRLVDKNGGLFYIPSESLSTPRANVADREYFTAQQDGQTRGFYISAPVQSR
                     VTGVWGIPVSYPISSGNVGMAVIFAAIELPPLNELYEAVRPKPSGAISLARRDGTLLD
                     RVPFDSTLMGTSLSRSSDSDEFYEGSRHFISPIDGETRIIAFHIMKDLPLMISVSVSK
                     SDIFSSWYERMLVWIVVLVAVSILLAALGLFLWSSWSINVQNSNLMSAMNEELTNANE
                     MARLVTENSTDVFTVLSLPEMIVEYLSPSVERNWGWKETELKGKTIEALLDAPTYTKV
                     RDTIDDMQKRLTMGESSARHGKLDINVAHKDGHQIPYELASTIICDNRSTPIKIVALA
                     RDLSERKANEDIVRAMAFYDRLTGLANRRLLEDRLVQLLPLTLREDKKLAILFIDLDR
                     FKPVNDTYGHEAGDWLLRQVADRMLASLRESDLVARTGGDEFIAVLPDVGTSDNASLI
                     ADKIRERISEAYPYDETNTFSISASIGVVLCPDHGRDTRSLLMLSDTAMYQAKKSGRN
                     RVVLYSSDTAALAGMHTSVSIIPWQDSYKSGNDEIDKQHRKLFQMASELIRLASQKDV
                     EKEECMDAIAHVEAHVQEHFHTEETMLFSLGYPDALMHKILHADLTKQLGVLKARYYK
                     EESTLDELVTFIAKTVIDEHLLNEDRRFFYLWRSDDLYNPTRGEHR"
     gene            complement(4302..5975)
                     /locus_tag="PKH81_00325"
     CDS             complement(4302..5975)
                     /locus_tag="PKH81_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNQ96521.1"
                     /translation="MKRILLLCLLVSFVISCYCESASVDSSRFIQVDGTMFRTPDGRP
                     WIGRGVAFGNSVWNNPSEATSFKHHRMEDYGRVKAMGFNSVRFYINYALFETDSRPGQ
                     YREQGFSWLDQNIAAARDQGVGLILNMHYPQGGFQSNGAGDALWNSKKNQDRLVALWK
                     EIAKRYRGEPVIIGYGLVNEPVPVHGIEEWEALAQRLITAIREVDAHHIIFVERAIWL
                     KSGSSNEISSRLFFPKGLNDPSRQLALEFHFYEPMGFTHQNTSWTGWKGLYASYPDEG
                     RIEVEDAQWAGFASASQALPAGNTSWRELGGTPVTCDNPSYLVAYPTVQTQGLGAKGR
                     VWIEELRVEASSDGGVTWSQLGLIEGSSLSGCDFWSSDGSGSLFLTPGKGKTDKTITV
                     SGTGGDANITFRGMPIVCDKNQTYRLTGRLRGESVSKTAMVRLRLDFYSGKSLTAWNK
                     RYLESRLASYITYARQQGVPLYLGEFGLASDAFREQRGGLNWVEDMLDILVSNNMSYN
                     YHTWKESAFGIYQNNSGYADPSRVNSPLEQAFIRMQNPERGSASLFSAP"
     gene            complement(6073..>6746)
                     /locus_tag="PKH81_00330"
     CDS             complement(6073..>6746)
                     /locus_tag="PKH81_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ96522.1"
                     /translation="TIGGVRYTSLLIALVVFIGYIIVEIISRRKKIRYNFDVLPPMLM
                     IGKFLMMGFFIFLLAWKFATYRGIPNVVIVLGITIGLFTFITKNTIIGRYIYAVGGNA
                     RSAKLSGINSEMVTFIVFIIMGAMCGLAGVIFTGYMNTALPQAGVNFELDAIASCFIG
                     GASTSGGIGTVMGAIIGGLVMAAINNGMSLMNIAAQWQYVVKATVLLLAVLYDIYTRR
                     KAGLG"
BASE COUNT         1775 a         1711 c         1482 g         1778 t
ORIGIN      
        1 gcgtttttct ttatactctt ttgtatgaaa gcccatcgaa tcactcttgc ttggtgtaag
       61 cttctatggc aaacccgcct tggtgttatt cttcaaagcg tacttattgg tattactacc
      121 ggttttgtag tccttgcctt tcggcttgcc ctaagccatg gagagcacgc aagatcgcgt
      181 gtctatacat tcctttcggt tcattcccca ttgtggacgc ttatatggat agccgttatt
      241 gtcgcccttg gattgctgct tggcctcttg tcaaaactac ggcctatgat aaaaggaagc
      301 ggaatcccgc agataaaagg cgaattgatg ggaaagcttt cccaccgctg gcttaccgaa
      361 ttacccttaa aatttatatc cgccgttctt gctattggcg cgggtctatc ccttggacgc
      421 gaaggcccct cggtccaaat gggtggatac atcggcaagg ctatttcccg acttggcaat
      481 cgatctcccg cagaactgcg tttccttatg attagcggcg cggctgcagg actttcggca
      541 gcctttaatg caccgcttgc aggagtcctc tttgccctgg aagaattaca aaaacatttt
      601 tccccgctca tgctcctgtg cgcaatagct gcctccgcca tgggtgactt tgtatcaagt
      661 catttttttg gattagcgcc cagtttttct ttccctgcaa ttaccccgtt gtctttaaat
      721 catttcccct ggctcatcat ccttggtctt ctttccgcct tttgcgggga cttgttcaag
      781 caaagtctgt atctatcgca aagtctgtac gcacgactag gcattcctcc aattcttaga
      841 ccagtactcc cacttcttgt ttccataccc cttggcttta ccctggccca aacgatcgga
      901 ggcgggcatg aactggttga gcagcttgcc cttgcccaat acagcgtgtt gtctatagcc
      961 ctgttctttt tggttaaact ggtattctcg ggtctttcgt acggagcggg aacggcaggt
     1021 ggtatcttct tgccattact ggtgtgcgga gcccttgtgg gaaccggttt tggaactgtc
     1081 ttacacacag cgggaattat taccaacatc gaaatcgtca attttttaat attgggaatg
     1141 gccgccttct ttaccgccgt tgtaagagcg ccggtaaccg gtaccgtact catcctggaa
     1201 atgagtggca atctttctca tcttggcggt cttgttattg tctgcctgat agcgcaactt
     1261 gcttgtgacc ttatgcgctc ccgaccagta tatgacgtaa tgttgtaccg acttaccagg
     1321 tcgtagttac tttacgccaa atgctatgcc ggcacgcccg gtggtgatca gccaatatgc
     1381 agtaaagatc cgcttttttg gcagcggagc aactggcaat ttccaatccc tttttccaca
     1441 atctcgttaa tgttttggtt gcttaccact gcgggtaatc caatttttaa tagaccaccg
     1501 tgcgtaacaa tcaaaacatt gtgatctgca ggagaataac tggagaaaag gtaatcaata
     1561 aattgcctga atcgctgctg tacctgatta aaggtttctc cgttaaaaag ggagctatca
     1621 cgattagatg tactaatcca ctgccgctca tttttaaaat acaaatccca actctgtgca
     1681 tcggacaatc cttcaagaaa tccaacatca tattccctta aaagtgagtt cacctcaaac
     1741 cgcggaatat gtaattggga gcaaacgata gaggctgtct caatcgcgcg caaaatcgga
     1801 cttgcataca cagaggtaat ccgaactccc tgtaataaga gagctaaatt ctccgactgt
     1861 tttttaccct tatccgttaa cggatgcttt agtcccctat tggagaacac atgctcaacg
     1921 ttagcggtgc tctccccgtg tcgggttata tagatcatct gatctccata aatagaaaaa
     1981 tcttctgtcc tcattgagca agtgttcgtc tataacagtt ttagcgataa acgtaacaag
     2041 ttcatcaagg gttgactctt ccttgtaata gcgcgcctta agaacaccca actgcttagt
     2101 caaatcagca tgaagaattt tatgcataag agcgtccggg tagcccagac taaaaagcat
     2161 cgtttcctca gtatgaaaat gctcctgcac atgcgcttct acatgagcga tggcatccat
     2221 acattcttct ttttctacat ccttttgtga agccaagcga ataagttcac tcgccatttg
     2281 gaacaatttc cggtgttgct tatctatttc gtcgttcccg cttttatacg aatcctgcca
     2341 gggtataatg ctcactgagg tatgcatacc cgcaagagcg gccgtatcgc ttgaatacaa
     2401 cacaacccga ttacgtccag acttttttgc ctgatacata gccgtatcgc tgagcatcaa
     2461 aagactacga gtatccctgc catgatcggg acaaagcaca accccaatac tcgccgatat
     2521 cgagaaagta ttggtttcgt catacggata tgcctcgctt atcctttccc gaattttgtc
     2581 cgctattagt gatgcattat cgctcgtccc tacatctgga agaacagcga taaactcatc
     2641 gccacccgta cgggccacca agtctgactc tcgcagcgac gcaagcatac ggtcagctac
     2701 ctgcctaagg agccagtctc ctgcctcatg gccataggtg tcgttaacgg gcttaaaacg
     2761 atcaaggtca ataaacagta tggcaagttt tttgtcctcc cgcagagtaa gcggcaaaag
     2821 ttggactaac cggtcttcaa gaagccgacg gttggcgaga ccggtaagac gatcataaaa
     2881 ggccatagcg cgaacgatat cctcattcgc cttacgttcc gacaaatccc gggccaaggc
     2941 tacaatctta atcggcgtac tccgattgtc gcaaataatg gttgacgcca attcatacgg
     3001 aatttgatgc ccatccttat gtgcaacgtt tatatccagt ttaccatggc gagccgatga
     3061 ctcacccata gtgagccttt tttgcatgtc gtctattgta tcgcgtacct ttgtgtaggt
     3121 tggcgcatca agcaaggctt caattgtctt tccttttagt tctgtctctt tccatcccca
     3181 atttcgctcc accgaagggc taaggtactc aacgatcatc tccggaagcg atagtaccgt
     3241 aaacacatcc gtgctgtttt ctgtaacaag gcgagccatc tcgttcgcat tcgtaagctc
     3301 ttcgttcatc gcgctcataa gatttgagtt ttgtacgttt atgctccagc tactccataa
     3361 aaaaaggcca agggctgcaa gcagtatgga tactgccacc aatacaacaa tccacaccag
     3421 catgcgctcg taccatgatg aaaaaatatc acttttggaa accgagacac taatcataag
     3481 tggtaagtct ttcatgatat gaaatgcgat tattctggtt tccccgtcaa tcggagagat
     3541 aaaatggcga gatccttcgt aaaactcgtc ggaatctgaa gaccttgaaa gggatgtgcc
     3601 cataagagta ctgtcaaaag gaaccctgtc tagaagggtg ccatcccttc ttgccaaact
     3661 tatcgctcca ctcggttttg gcctgacggc ttcgtagagc tcattaagtg gaggcagttc
     3721 aatggcagca aaaatgacgg ccatccccac gtttccggag gaaatgggat acgacaccgg
     3781 tattccccat acaccggtaa cccggctttg aaccggcgcg ctgatgtaaa aacccctggt
     3841 ttgcccatct tgttgagccg tgaagtattc acggtctgca acattcgcgc gtggagtact
     3901 cagactctct gacggaatat agaaaagacc gccatttttg tcgacaaggc gtatatcgat
     3961 agggatgcga ttgttttgcc gaataacagt aacaaggtct ataaagtcct tatctgttcg
     4021 aggatctttg tccggatttg ccgctagcca ggtttccaac aagatgagct gaccctttac
     4081 ctctctaaag agacttcccg tttgttcggc tacgagctgg ttaagacgta ctaattcgtc
     4141 ctcggtttga gcaagttccg agtttctctg gctaattccc gctactgccg caatagccca
     4201 aattgctgct atggcaatta aaacgaatgt tattataaaa gaagagcgat cttttatttt
     4261 tgcaaacttg ggtgtattca tgtaggtata agtataagac gctagggtgc cgaaaacaag
     4321 ctggcactcc cgcgctccgg gttctgcatg cgaataaagg cttgctctag cggggaatta
     4381 acccgggaag gatctgcgta gcctgagtta ttttgataga tgccaaaggc gctttctttc
     4441 catgtatgat agttatagct catgttattt gatacaagaa tatcgagcat gtcttccacc
     4501 cagttaagcc ctccccgctg ttcccgaaac gcatccgaag caagcccaaa ctcgccaaga
     4561 tacaggggca cgccttgttg acgggcatag gttatatagg aagcaagacg actttccaga
     4621 tagcgcttgt tccaggcagt aagagatttt ccggaataaa aatcaaggcg taacctcacc
     4681 atggcagtct ttgacacaga ctccccgcga agtcgtccag taaggcgata tgtctgattc
     4741 ttgtcgcaca caatcggcat tccccggaag gttatgttcg catccccgcc tgtcccgctc
     4801 acggtaatgg tcttgtcagt ttttcctttg ccgggggtaa gaaatagaga acctgaccca
     4861 tcacttgacc aaaaatcaca gccagagagg gatgaaccct ctatcagtcc aagctggctc
     4921 caggtaacac cgccatcgga agaagcttct actcgaagtt cctctatcca caccctgccc
     4981 tttgcgccca gaccctgtgt ttgaactgtc gggtatgcga caagataaga agggttatcg
     5041 caggttaccg gggtccctcc caattcacgc caagacgtat tacccgcagg aagcgcttgg
     5101 ctagcgctgg caaaccctgc ccactgagca tcttccactt ctatgcgccc ctcatcggga
     5161 tatgaggcgt agagcccttt ccatccagtc cacgaggtgt tttgatgagt aaaacccatc
     5221 ggttcataaa aatgaaattc cagggcaagc tgacgggagg gatcgttaag cccttttggg
     5281 aaaaaaagtc gggaagaaat ctcgttggaa gagccgcttt taagccagat ggcacgctcg
     5341 acaaagataa tgtgatgcgc gtccacctcg cgaatcgcgg taataagacg ctgggcgagc
     5401 gcttcccatt cttctattcc atgaaccggc accggctcgt ttaccagtcc atagccgata
     5461 atgactggct ctccccgata ccgtttggca atttctttcc aaagagcgac cagacgatct
     5521 tgattttttt tggaattcca caaagcatcc cccgctccat ttgactgaaa tccgccctgg
     5581 ggatagtgca tattaagaat caacccgaca ccctgatctc tggcggcggc gatattttga
     5641 tcaagccagg aaaatccttg ctcccgatac tggcccggac gagagtcagt ttcaaaaaga
     5701 gcgtagttaa tgtaaaagcg taccgagtta aaacccattg ccttaacccg gccataatct
     5761 tccatgcgat gatgcttaaa agaggtagct tcgctcgggt tattccacac agaattccca
     5821 aaggctacac cacggccaat ccatggcctt ccatccggag tgcggaacat agtgccgtct
     5881 acctgaataa agcgggaact atcaactgaa gcggactcgc aatagcaaga aataacaaag
     5941 gaaaccaaga ggcacagcaa caagattctt ttcataccaa agagtataca ccgttattgt
     6001 gtatcgcata aaaaaaagcc cgtagtctcg aggaaagacc acgggcggtg aggggcttaa
     6061 cccttcagaa gatcaaccca aaccagcctt gcgccgggta taaatatcgt aaagaacggc
     6121 gaggagaagg accgtcgcct ttacgacata ttgccattgc gctgcgatat tcatgagtga
     6181 cattccgtta tttattgctg ccataaccag accaccgata atggcaccca taaccgtacc
     6241 aattccacca gaggttgacg caccgccgat aaagcaggaa gcgatggcgt ctagctcaaa
     6301 gttaacacct gcttgaggaa gtgcggtgtt catatacccg gtaaagataa caccagctag
     6361 tccgcacatg gcacccatga taataaagac gataaaggta accatttccg agttaatacc
     6421 ggaaagtttg gccgaccgcg cgttaccacc tacggcgtag atgtagcgac caataatcgt
     6481 attcttggta ataaaggtaa acaagccgat tgtaatacca agaacgatta ctacgttggg
     6541 aataccgcga tacgtagcaa atttccaggc aagcaagaag ataaaaaagc ccatcatcag
     6601 gaactttcca atcatgagca ttggaggaag cacatcaaaa ttatagcgaa tttttttcct
     6661 tcgagaaatt atctctacga taatatagcc aatgaaaaca accagagcta taagcaaaga
     6721 tgtgtaacga acgccaccga tagtaa
//
LOCUS       DAOQXQ010000010         6477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_12323_length_6477_cov_3.494861, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000010 DAOQXQ010000000
VERSION     DAOQXQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_6477_cov_3.494861"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKH81_00335"
     CDS             <1..185
                     /locus_tag="PKH81_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96523.1"
                     /translation="ARELRRHDVPVELHVYRRGKHGLSSATANTVNTKGWGIETTCQN
                     WLDMAITWINDFNRDS"
     gene            complement(187..840)
                     /locus_tag="PKH81_00340"
     CDS             complement(187..840)
                     /locus_tag="PKH81_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ96524.1"
                     /translation="MDMNEPFEFPLNRYLTAAERERVKQHLIISEVDAGTVLFDATEC
                     RGVLVVIRGIVRVYNLSESGREITLYRLFENDICTLSVSCLLGSMPLQAMVKADTDCT
                     IASLSNDVFSDLHRNNSQIQQFLLTNMSARISDVMWVVEQVAFASMDRRIGEFLLSQP
                     SMVVYVTHDKIAQELGTAREVVSRMLKYFEKNGYIELSRGKLKIIDPEGLKRSFSIQ"
     gene            935..1942
                     /locus_tag="PKH81_00345"
     CDS             935..1942
                     /locus_tag="PKH81_00345"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HNQ96525.1"
                     /translation="MKTRFSCHLTGRDWWKPFLVFFAVLVFLNLQMQDASSKITLATK
                     GRELTSIILYYFVIYALFILVQAASLIILSSRALPKIVLGDTCSSFHGKMLPYLGINI
                     KGFLLTLITLGLYYPWYSRSLLAYYYSSVTIQDAPLIFSGTAKKLFKYFIFGLLLPLL
                     VWSVAFGITFGMAILSYTTTGEIDTSHVFLTILLFFSLFPLLAPFLYLSLRWSLSVQW
                     KSRTLVINITFWDGLFFFIKQIFLACITLAIYLPAVYINTYRFFASQSTLAEEGQEPI
                     IIGFDGTNREGFGFIWKQILLSLITCGIYFPWAYAKILCFFINGTWLDEKSADTTALE
                     A"
     gene            complement(1970..2167)
                     /locus_tag="PKH81_00350"
     CDS             complement(1970..2167)
                     /locus_tag="PKH81_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNQ96526.1"
                     /translation="MAKRLYKVEFGRKIFGVCGGLAEYLNVDVVIVRIVWGVLALMYG
                     FGLILYLVCAFVLPNKSEVEH"
     gene            complement(2261..2854)
                     /locus_tag="PKH81_00355"
     CDS             complement(2261..2854)
                     /locus_tag="PKH81_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNQ96527.1"
                     /translation="MLRKIVTITLIAFVVGTAFISCSSAPEEKEEVPVTRKIIGQDGV
                     PMPDWVNKNPKSETHFYAVGYAMKSSRAISKTASGQDARDQIARWVGTSVKNALVSYT
                     SEGGEQTNTQTLSHFENISKQVAEQTLVGVEEDEIWVDDKGGVYMLCSFPKANLNKSF
                     EETVGTFQRNEAAAFAEFKAKEALSFLEKETADGVQK"
     gene            2983..3378
                     /locus_tag="PKH81_00360"
     CDS             2983..3378
                     /locus_tag="PKH81_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96528.1"
                     /translation="MTTRKKDILIIAILLMCTALVRLWTTMMIHTGIDERDYWYSAKA
                     ISQGFDYIHLNHRTIRWSVIIPVAAVQALCGVHPNVYYILPFINALLQTSLLYLLGKR
                     LFSRKVAVLAVFFLIFFPYQIKPAVIKWL"
     gene            3483..3770
                     /locus_tag="PKH81_00365"
     CDS             3483..3770
                     /locus_tag="PKH81_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96529.1"
                     /translation="MMYRPLQFFCGIGSILFLLGFALGLRFLYYFLTETGPNGHIQSL
                     ILAAVLMMLGFQTIVTGLQADIIAANRKILEDIQKKVRNIDIQLQGKDDAH"
     gene            3760..4194
                     /locus_tag="PKH81_00370"
     CDS             3760..4194
                     /locus_tag="PKH81_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNQ96530.1"
                     /translation="MLTKVVNRETISYVLFGVLTSLLNIGIFHGLILVSVDYKLANIV
                     ALVTTKITAYLCNKFFVFKSQTTTGTALAGEIIRYTAVRGSTLFIDYFGLILLVSQFN
                     IDKTLAKVCVTFIVVLINYLLGKKWVFLGKNPEPHSPESPLA"
     gene            4287..>6477
                     /gene="nifJ"
                     /locus_tag="PKH81_00375"
     CDS             4287..>6477
                     /gene="nifJ"
                     /locus_tag="PKH81_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNQ96531.1"
                     /translation="MSEKKLVMIDGNTAAGHVAHALSEVIAIYPITPSSPMGEVADEY
                     SAKGKKNIWGTVPEVVELQSEGGAAGAVHGALTTGALSSTFTASQGLLLMIPNMYKIA
                     GELTSTVFHIAARALAASSLSIFGDHQDVMACRQTGWAMLASNNVQEVMDLAVISHAA
                     TLESRVPFLHFFDGFRTSHEVQKIEEVPYDVMQKMVDDELVRAHRARGLTPENPSIRG
                     TAQNPDVYFQGREAVNPYYTKVPAIVQKAMDKYAQLTGRQYKLYEYVGAKDADRVVIL
                     MGSGCDTVEETVEALVAKGEKVGLLKVRLYRPFSAEDFVKTLPATVKAIAVLDRTKEP
                     GSLGEPLYEDVRTAIGEMQAAGKCQFKDYPTVIGGRYGLGSKEFTPAMVKGVYDNLKG
                     EKKPNFTIGIEDDLTNSSLKYDPSFQLEDAGMHQAMFYGLGSDGTVGANKNSIKIIGE
                     ATDNKAQAYFVYDSKKSGSITTSHLRFGKHAIRKPYLVTQSDFIACHKFTFLEKYDML
                     STAKKGATFLLTSPYGKDQVWDHLPVEVQKQIIDKELKFYVIDAVTIAEKAGMGGRIN
                     VVMQTAFFKISGILPEAEAVALIKKFIEKSYGKKGADVVKKNIDTIDMALAGVEKVEY
                     PKTASSKLKMLPAMTADAPEFVQKVLGTIAVNKGDDIKVSQVPNDGTFPSGTTQYEKR
                     AIAERMPIWNKDLCIQCGQCTVVCPHAVIRAKAYDPKFLAKAPASFQS"
BASE COUNT         1778 a         1602 c         1270 g         1827 t
ORIGIN      
        1 acgcaagaga actccgccgc catgacgtcc ctgttgaact acatgtatat cgccgtggaa
       61 agcatgggct gtctagcgcc acggcaaaca ccgtaaacac caagggctgg ggcatagaaa
      121 caacctgtca aaactggctg gatatggcaa ttacctggat caatgatttt aatcgcgatt
      181 cctaggctat tgaattgaaa aactgcgttt taaaccctcg gggtcaatta tcttaagctt
      241 cccccgagaa agctctatat atccattttt ttcaaaatat ttaagcattc gactgacaac
      301 ttcgcgcgcg gtccctaact cttgggctat tttgtcgtgt gttacataca caaccatact
      361 gggttgagac aaaagaaatt ccccaatgcg tctatccatc gaagcaaaag caacctgttc
      421 tacaacccac ataacatcgc ttatacgggc actcatattg gtaagaagaa attgttgtat
      481 ctgagaattg ttccgatgaa gatcagaaaa aacatcgttt gataaacttg ctatcgtaca
      541 atcagtatct gccttaacca ttgcctgtaa gggcattgaa cccaaaagac aggacacaga
      601 cagagtacaa atatcatttt caaataaccg atacaaggta atctcacgcc cagattcaga
      661 caagttgtat acccgcacaa taccccgaat aacaactagc acccctcgac attctgttgc
      721 atcaaataag acagtacctg catctacctc ggagattatt agatgttgct tgactctttc
      781 acgctctgcc gcagttagat atcgattcaa tggaaattca aagggttcat tcatatccat
      841 agttatatcc cccagggttt tacttgacaa gcaataaaca ggaaggattg ttgctgtaaa
      901 acctccatgg tatactaaga aaaggagttt tatcatgaaa acacgttttt cttgtcacct
      961 aaccggacga gattggtgga aacctttctt ggtgtttttt gcagtgcttg tgtttctcaa
     1021 tctgcaaatg caagatgctt cgtccaaaat tacactagcg accaaaggaa gagaactaac
     1081 ctctattatt ctctattatt ttgttatcta tgcccttttt attcttgtac aggccgcatc
     1141 acttattatt ctctcttcca gagctttacc aaaaatcgta cttggggata cctgcagttc
     1201 ttttcatggt aagatgcttc cctacctggg aatcaatatt aaaggctttt tattaaccct
     1261 tatcaccctt ggcttgtatt atccttggta ttctcgctcc ttgctggcgt attattattc
     1321 ttcagttaca attcaagacg ccccgcttat attttctggt accgccaaaa aactgtttaa
     1381 gtactttatc tttggactgc ttttaccact tcttgtttgg tctgtagctt ttggcattac
     1441 ctttggtatg gctattcttt catataccac aaccggtgaa attgatacgt cgcatgtttt
     1501 tttgacaatt cttctctttt tttccctttt ccccctgtta gcgccctttc tttatttatc
     1561 tctccgttgg tctctatcag tgcaatggaa atccagaacc ttagtcatca atattacatt
     1621 ctgggatggt ctattctttt ttatcaagca aatctttctt gcttgtatca ctcttgccat
     1681 atacttacct gcagtatata tcaacaccta ccgttttttc gcatcacaaa gcacccttgc
     1741 ggaagaaggg caagaaccga ttattatcgg ctttgatgga acaaaccgag aaggcttcgg
     1801 ttttatttgg aaacaaatcc ttctttcact tattacctgt ggtatctatt ttccctgggc
     1861 atacgccaaa attctctgtt tttttatcaa tggaacatgg ctagatgaaa aaagcgcgga
     1921 tacaaccgcg cttgaagcgt aatacgatgt aaagtggagt gctgatgatt cagtgctcca
     1981 cttcgctctt gtttggaaga acaaaggcac aaaccagata caaaattagt ccaaacccgt
     2041 acataagggc taaaacaccc catacaatac gaacgataac gacgtcaaca ttcaggtact
     2101 ctgccaaacc accacaaacg ccaaatatct tccgtccgaa ttcaaccttg taaagccttt
     2161 tcgccatatc tccctcctgg cccaaaaaaa agccctgcgg tctatattcg acagcagggc
     2221 acaacgccac gggtctatcc ccgcaacgcg gatacactca ttatttttgt acgccgtccg
     2281 ccgtttcttt ttccaggaag gacagggctt ctttcgcctt aaattcggca aaggccgcgg
     2341 cttcgtttcg ctggaaagta ccaacggttt cctcaaagct cttgttcaga tttgctttgg
     2401 ggaaagaaca cagcatatac acaccaccct tgtcatcaac ccaaatctcg tcttcctcta
     2461 caccaaccag ggtctgttct gccacctgct tggatatatt ctcaaaatga ctgagagtct
     2521 gagtgttggt ctgttccccg ccctcgctgg tatagctaac cagagcgttt ttaacactgg
     2581 ttccaaccca tcgggcaatc tggtctcgag cgtcttgtcc cgaggcagtc tttgatattg
     2641 cccgagaaga cttcatcgca taacctacag cgtaaaaatg ggtttcactc ttggggttct
     2701 tgtttaccca gtctggcatc ggcactccat cctggccgat aatcttgcgg gttacgggaa
     2761 cctcttcctt ttcttccggt gcggaagaac aagaaataaa cgcggttcca acaacgaatg
     2821 caatcagcgt aattgttaca atttttctta acatcacttc tcctcctggt attattccag
     2881 tgtttcacta agtgtaacac actcaaaaca aaaaacaatc accctctttc gtacgattct
     2941 atttgctttt atactataca tactttgcct tatggagtaa ttatgacaac ccgcaaaaaa
     3001 gacatcctga tcatcgcaat cttgcttatg tgtacagccc tggttcgttt atggacgacg
     3061 atgatgatac ataccggaat tgatgaacgg gactattggt actcggccaa agcaatcagc
     3121 cagggctttg attacatcca tttaaaccac cgaaccatta gatggtctgt tattatacca
     3181 gtggcagccg ttcaggctct ctgtggcgtt catccaaatg tgtattacat cctcccattc
     3241 attaatgccc ttctacaaac aagcctgttg tatcttcttg gtaaacgcct tttttcccga
     3301 aaggttgcgg ttctggctgt gttttttctt attttttttc cttatcaaat caagccggcc
     3361 gtaataaaat ggctataacc tctgttccca tacacacaaa tccagaatta cgaaaatccc
     3421 gccttttttc cagcatgttt caatatgtca aacggtcctc ttttactata attcgggctt
     3481 ttatgatgta ccgccctctt caatttttct gcgggatagg atcaatcctg tttctccttg
     3541 gtttcgccct tggattacgc ttcttgtatt attttcttac cgaaacaggg cctaacggtc
     3601 atatacaatc gcttatcctt gcagccgttc ttatgatgct cgggtttcaa accattgtta
     3661 ctggtcttca agccgacatc attgccgcaa atcggaaaat tctggaggat atacaaaaaa
     3721 aggtaagaaa tatcgatata cagcttcagg gaaaggacga tgctcactaa agtagttaac
     3781 cgcgaaacga tctcttatgt cctttttggt gtattaacca gccttctcaa cataggaata
     3841 tttcatggat tgatccttgt ttcggtagac tataaactcg ccaatattgt cgccctcgta
     3901 acaacaaaaa taacagcata tctctgcaat aaattctttg tattcaaatc tcaaaccacc
     3961 accgggacag ctttagccgg agaaattatt cgttatacag ctgttcgggg aagtactctt
     4021 tttattgact actttgggct tattctttta gtttcccaat tcaacataga taagaccctg
     4081 gctaaagtat gtgtcacctt tatagttgtt ttgatcaatt atcttctcgg aaaaaaatgg
     4141 gtgtttcttg gaaagaaccc agaaccacat tcaccggaaa gtccacttgc ataaaccacc
     4201 cgtacagggt attttatagc gtataaaggt aattttttac ctatatcata catctctcac
     4261 ttcaattctt ttataaggac ggaaatatgt ctgagaaaaa acttgtcatg atcgacggaa
     4321 acactgccgc cggtcacgtg gctcatgcct tgagcgaggt tatcgcaatc tacccgatta
     4381 ccccttcaag ccccatggga gaggtagccg atgaatactc ggccaaggga aaaaagaaca
     4441 tttggggaac tgtccccgaa gttgtagaac tccagtccga aggcggagcc gcaggcgcgg
     4501 tccatggagc tcttaccaca ggagcgcttt catcgacgtt taccgcgtca cagggtcttt
     4561 tgctcatgat cccgaacatg tacaagattg ccggcgaact aaccagcacg gtatttcaca
     4621 tcgctgcacg cgcgcttgct gccagctccc tctcaatttt cggtgatcac caggacgtaa
     4681 tggcctgccg gcaaaccgga tgggcaatgc tcgcatctaa taacgtccag gaagttatgg
     4741 atcttgcagt tatttctcac gccgcaaccc ttgaatcacg cgttcctttc cttcactttt
     4801 ttgatggttt ccgtacctcc cacgaagttc aaaaaatcga agaagtccct tacgatgtta
     4861 tgcaaaagat ggtggatgac gagcttgttc gtgcccatcg cgcacggggc cttaccccgg
     4921 agaacccctc catccgcggt acagcgcaaa accccgacgt ctacttccag ggacgcgagg
     4981 ccgtaaaccc ctactacacc aaagtccccg ctattgttca aaaagcaatg gacaaatacg
     5041 cacagctcac tggccgccag tacaaactct atgaatacgt aggtgccaag gatgcagatc
     5101 gagttgtaat ccttatgggt tcaggctgcg atactgttga agaaactgtc gaagctcttg
     5161 ttgccaaggg agaaaaagtc ggactcctta aggttcgtct gtatcgcccc ttcagcgcag
     5221 aagactttgt aaaaactctc cccgctactg ttaaagcgat tgccgttctt gatcgaacca
     5281 aagaacccgg atctcttgga gaacccctct acgaagatgt tcgcaccgct attggcgaga
     5341 tgcaggcggc cggtaagtgc caatttaagg attaccccac tgttatcggc ggacgctacg
     5401 gactcggttc aaaagaattt acccctgcca tggtaaaggg tgtatatgac aatcttaagg
     5461 gcgaaaagaa gcccaacttt accattggta tcgaagatga ccttaccaac tccagcttga
     5521 agtatgatcc gagcttccag cttgaagacg ctggtatgca ccaggccatg ttctacggac
     5581 tgggatccga tggaaccgtt ggcgcgaaca agaactccat caagatcatt ggtgaagcta
     5641 ccgacaacaa ggctcaagca tactttgtgt atgactccaa aaaatcggga tcaatcacta
     5701 ccagtcacct acgctttggt aagcatgcga ttcgcaagcc ctacctggta acacagtcag
     5761 actttattgc ttgtcacaag tttacattcc tcgaaaagta cgacatgctt tctaccgcaa
     5821 aaaaaggcgc aaccttcctg cttaccagcc cctatggtaa ggatcaagtt tgggatcacc
     5881 ttccggtaga agtacaaaag caaattatcg ataaagaact caaattctat gttatcgacg
     5941 ctgttactat cgcggaaaag gctggtatgg gtggacgtat caacgtagtt atgcaaaccg
     6001 ccttcttcaa aatttccgga attcttcccg aagcagaagc tgtagccctt atcaagaaat
     6061 tcattgaaaa gtcctatggt aagaagggtg cggatgtagt taagaagaat atcgacacca
     6121 tcgacatggc cctggccgga gtcgaaaagg ttgagtatcc caagaccgca tccagcaagc
     6181 tcaaaatgct tccggcaatg acagctgatg cacctgaatt cgtacaaaaa gtacttggaa
     6241 ccatcgctgt taacaaaggc gacgacatca aagtaagcca ggtaccgaat gacggaacct
     6301 tccccagcgg aactacacag tacgaaaaaa gagccattgc agaacgcatg cccatttgga
     6361 acaaggacct ttgtatccag tgcggtcagt gtactgttgt ctgtccacac gcagttattc
     6421 gggctaaggc atatgatcca aaattccttg ccaaagctcc tgcctccttc cagagtg
//
LOCUS       DAOQXQ010000011         6469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_12339_length_6469_cov_2.821640, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000011 DAOQXQ010000000
VERSION     DAOQXQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_6469_cov_2.821640"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PKH81_00380"
     CDS             complement(<1..1242)
                     /locus_tag="PKH81_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96532.1"
                     /translation="MTKTVLSLCFTCVFFLYVSCTSTGPATTPEQEVPAEEQHTVTAV
                     EAQESEASVSDQSEENISSEVPQVSPGDIADPKETDDFIYFYPEPSLLLPEPTHTQDD
                     SKLVQEPPQELPKEVKIERKKEPEAKPEAKPEAKPEGTAEVEPDVAQEPLETPEPGIW
                     EKESVPPSVSPTIQSTPVKKATREVTVPRDFSLEVWYPGTGWVYLGDSSSLDGLSYQT
                     RKIDGNDTLFSFRALKEGSYLLEFSRYDVLGDSFVSDLLAVQVKEPGNIRSGKVRAPD
                     YRSLTDSSIVETAPDPVHSVMIDEPELRPVTTPVQEAARSVPVPTEDPLLLLEEARKA
                     IAQADSVRALEYLDSFFTVARASLDEGWFLRAQAYEANTPLRDIRKALSAYETVTSVF
                     PDSVRWKESDERIRYIKQFYFR"
     gene            complement(1239..1607)
                     /locus_tag="PKH81_00385"
     CDS             complement(1239..1607)
                     /locus_tag="PKH81_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96533.1"
                     /translation="MSQLEALTNFFETHKKRCVIVLSVLLLIFIGMLIFQISVSSQQK
                     SQMRIEQEQLRSQRVPVTDVWLLPDPLPLPGIQFSRDQKKQWGPQEQNLWYSEINEQD
                     VAAVGSEARRQIDALLESVP"
     gene            complement(1607..4549)
                     /locus_tag="PKH81_00390"
     CDS             complement(1607..4549)
                     /locus_tag="PKH81_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96534.1"
                     /translation="MFLKSLDIFGFKSFADRTHIQFSDGITALLGPNGCGKSNVVDAV
                     KWVLGEQASKAMRADKMEDVIFNGTESRKPLNVAEVTLTIANEAGLLNLDVSEIAIKR
                     RLYRSGESEYYINNTPVKLKDIRELFWDTGVGKAAYSVMEQGKIDQILSSKPEERRYI
                     FEEAAGITRFKVRGAEAERKLSKTEENMRQIEGILGEVKRSYDTLKIQADKTVKYRTL
                     REAIFENELDIQLLRLKGFTQDKDRRSQDISDIQTKRSTVQAEIDEINASLEENMDVV
                     NSMEERLVDYQKQIYGLAVEKNEKDKQSHLLVERQNEVKSKLAQLEGRKTAIENRVES
                     LREDADGQDELVRDLNKRLAEIEKNISSFEQNIELAGNRISENDRQGAILESEIDSCD
                     REREIMESELRAITEDIVTELDQRLRDAGYSSRAHSEADEALSSIVSQIKILVSGKKN
                     IFSDFAQLHDPSRDDTKRFAESAVASFSELHELSGKLENALNSYRTTSPGFIDEFLSP
                     EGIITRKRSIDAKIQENREGISVRREKIATLKAVNTDLVVKIEEYRKTLEDLRLNRVQ
                     MKTQGEAAEEQARLIRRELASQEAALRELENELFTEQKRLEDILEQIDEIEGELASIE
                     HRGRTLTSELEKLEQDISSRNTDVSSKQSRLKDRTAEMGKIQSQLEKIHMDLAMTETE
                     IRNIMENFRETHSRDLMEFEERMFTINTPAGELREKLASSRSALRDLGSVNLMAVEEF
                     AEAKERYDFLTSQIADLQKARDDLKRITEEIRAESTEMFLATYNKIKKNFHNMFRRLF
                     GGGRGEIRLVDPQNVLESGVDIFAQPPGKRLENISLLSGGEKSMTAVALLFATYMVKP
                     SPFCLLDEIDAALDEQNVTRFVNALREFANVSQYIVITHNKKTVMGAGTMLGVTMEES
                     GVTKVIAIKLENESDRIVETTSDLENLEEEDVPLEENIVIPPHPPKRIKNAEVEENNT
                     DDSGESEA"
     gene            4721..5431
                     /locus_tag="PKH81_00395"
     CDS             4721..5431
                     /locus_tag="PKH81_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ96535.1"
                     /translation="MAETLVLFSSDTPGESQICRSLRRAGYTVKRVHTLSALHTIAYS
                     FTVLAVVLNRTIIERYCLEPIKHLKRARSACAIISWHSGLYHPEVDSVSVLCEYSTNG
                     WTQERRIEELDHVVKIIENPEKDEVQGDLFVTDETTRYGKRGLVTETDIPLEKTHKKM
                     RKVYELLVDAGSKGISVEDLSIRLWGRNAQIKRDDLQSYMSKLRKLLTEASGGKRTIA
                     RKGETYRISPIHNETAEQ"
     gene            complement(5465..5902)
                     /locus_tag="PKH81_00400"
     CDS             complement(5465..5902)
                     /locus_tag="PKH81_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ96536.1"
                     /translation="MISKQDFPAINERPPEGLKADGTPYRVLVVDDSMFVAKQIGQIL
                     TSEGYEIVTTATDGLDGVEKYKELCPNIDLVTMDITMPKMDGITALEQIMAFDKNAKI
                     VMISALGKEELVKKALLLGAKNYIVKPLDRKKVLERISTVLGK"
     gene            complement(5920..6384)
                     /locus_tag="PKH81_00405"
     CDS             complement(5920..6384)
                     /locus_tag="PKH81_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNQ96537.1"
                     /translation="MRVEYINPFVEAAYNILTEVLGGEIKRGELYLKSTSMPVMGVAA
                     LVGLAGDVEGRVIFDMSLDSAMKIASQMNGEQLQTFDDLAKATITELANLITAQAVTK
                     LHDLGFRFDLTPPALFTGDNMEISDHEVEALIVPMETDQGKVEVNVAIRDRM"
BASE COUNT         1681 a         1600 c         1383 g         1805 t
ORIGIN      
        1 gcggaaataa aactgtttga tgtatcgtat tcgctcatca gattctttcc agcgaacgct
       61 gtcgggaaaa accgaagtaa ctgtttcgta ggcagaaaga gcctttcgaa tatcccttag
      121 gggagtattt gcctcataag cctgtgcgcg caaaaaccat ccttcgtcca aagaggctcg
      181 ggcaacggta aaaaaagaat ccagatactc aagggcgcgc acagaatccg cctgagctat
      241 ggccttccgg gcttcttcca gcaagagaag cggatcctct gtgggaaccg gcacagatcg
      301 tgcggcctcc tgcaccggtg tggtaactgg ccgcaattca ggctcatcga tcattactga
      361 atgaaccgga tctggagccg tctctacaat agatgagtcg gtcaaagagc gataatcggg
      421 ggcgcgtacc ttaccggagc gaatgtttcc cggctcttta acctgaacag caagcaaatc
      481 cgatacaaat gaatcgccca agacatcgta gcgagagaac tcaagcaggt atgaaccctc
      541 cttgagcgcc ctaaaggaaa acaaggtatc attgccgtct attttccgtg tttgatacga
      601 aaggccatcc agggaggagg aatcgccaag atatacccag ccagtgccag gataccaaac
      661 ctccagcgaa aaatcccgcg gaacagttac ctcgcgcgta gcttttttaa ccggggtact
      721 ttgtattgtc ggactaactg aaggcggcac gctctccttc tcccaaatac ctggctccgg
      781 tgtttccaaa ggctcttgtg caacatcagg ctcaacttct gcagtacctt caggttttgc
      841 ttcaggtttt gcttcaggtt ttgcttcagg ttcttttttt ctttctatct taacttcttt
      901 gggtagctct tgtggtggtt cttgcacaag cttactgtcg tcttgggtgt gcgttggctc
      961 cgggagtagt aaagaaggct cgggataaaa atagataaaa tcatccgttt ctttcgggtc
     1021 cgctatgtcg cccggggaga cctgtggtac ctcagaggat atattctctt cggattgatc
     1081 gctaacagat gcttcactct cttgagcttc caccgcagtt acagtatgtt gctcttccgc
     1141 gggcacttct tgctctggtg tagtcgctgg tcctgtagag gtacaggata catacaaaaa
     1201 aaacacacac gtgaagcaaa gcgagagaac tgttttagtc atggaacaga ctccaataac
     1261 gcgtcaatct gtcgacgggc ttctgagcca acagcggcaa catcctgctc attgatttca
     1321 ctataccaca gattctgctc ttgtggtccc cattgctttt tttgatcacg ggaaaactga
     1381 attccaggaa gaggcaaagg atccggtaaa agccatacgt ctgtcaccgg aacccgttgt
     1441 gaacgaagct gttcctgttc aatacgcatc tggctttttt gttgtgaaga aactgatatt
     1501 tgaaaaatca acattccgat aaaaatcaat aaaagtaccg acaaaacaat aacacacctt
     1561 tttttgtgtg tttcaaaaaa attcgtcagc gcttcaagtt gactcattat gcttctgatt
     1621 ctccactgtc atctgtattg ttctcctcca cctcggcatt ttttatgcgt ttgggagggt
     1681 gtggaggaat aacgatgttt tcttcaaggg gaacatcttc ttcctcaaga ttttcaagat
     1741 cagaggtcgt ctcaacgatt cggtcagatt cattttcgag tttaattgcg ataaccttgg
     1801 taacaccgga ttcttccatg gtaacaccaa gcatagttcc cgcacccatt acggtcttct
     1861 tgttatgggt aataacgata tactgactta cgttggcaaa ttccctcagg gcattaacaa
     1921 agcgggtaac gttttgctcg tcaagagccg cgtcaatctc gtccaacaga caaaagggag
     1981 agggcttaac catataggtt gcaaacaaaa gcgctacagc agtcattgac ttttcgccac
     2041 cggagagcag actaatgttt tcaagccttt ttcccggagg ctgggcaaaa atatcaaccc
     2101 cggactcaag tacattttgg ggatcaacaa ggcgaatttc gcctcgaccg ccaccaaaaa
     2161 gacgcctgaa catattatga aagttctttt taatcttgtt ataggtagca aggaacatct
     2221 ctgtagactc agctctaatt tcctcggtaa tacgtttaag atcatcgcgc gctttttgaa
     2281 gatcagcaat ctgcgatgtc aagaaatcat agcgttcctt tgcttcagcg aattcctcca
     2341 ctgccatcaa gtttacactt cccaggtccc gtaaggcaga acgggatgaa gcgagctttt
     2401 ccctgagttc tccagcgggt gtattgatgg taaacatacg ctcttcaaat tccatcaaat
     2461 cccgtgaatg ggtttcccgg aagttttcca tgatatttct aatctcggtt tcggtcatcg
     2521 caagatccat gtgaatcttt tctaactgac tttgaatctt tcccatttcg gcggttcggt
     2581 cttttaaccg tgactgctta ctcgacacat cagtgtttcg gcttgaaatg tcctgttcaa
     2641 gtttctccag ttcagaagtc agggttcgac cgcgatgctc gatggaagca agctctcctt
     2701 caatctcgtc aatctgttca agaatatctt ccagccgctt ttgctcggta aaaagctcgt
     2761 tttccagctc gcgaagagcc gcttcctgcg aggcaagctc ccttcggatt aaccttgcct
     2821 gctcttctgc ggcttctccc tgagtcttca tctgcacacg gttaaggcgc aaatcctcaa
     2881 gcgtcttgcg atattcctcg atcttgacaa caagatcggt atttaccgct tttagggtgg
     2941 caatcttttc gcggcgaaca gaaatccctt ccctgttttc ctgaatcttt gcatcaatgc
     3001 ttcgctttcg cgtaataata ccctcgggag aaaggaactc atcaataaag ccaggggatg
     3061 ttgtgcgata ggaattaagc gcattctcga gtttgccaga cagttcatgc aattcggaaa
     3121 aactggcaac cgcgctttct gcgaatcgct tggtatcatc tcgcgaagga tcatgtaatt
     3181 gagcaaagtc cgaaaaaata tttttctttc ccgaaacaag aatcttgatt tgagaaacga
     3241 ttgaggagag agcctcgtct gcttcgctgt gtgcacgcga agaataaccg gcatcccgca
     3301 aacgctgatc aagttcagta acgatatcct cagtaattgc gcgaagctca ctctccatta
     3361 tttcgcgctc acgatcgcaa gagtctatct ccgattcaag tatggcaccc tgccgatcgt
     3421 tttcggatat gcggtttcct gccaattcaa tattctgctc aaaagaggag atattttttt
     3481 caatctcggc aagccgtttg ttcagatcgc gaactaattc atcttgacca tctgcgtctt
     3541 ctcgaagact ttcaaccctg ttttctatcg cggtttttct gccctcaagc tgggctaatt
     3601 ttgatttaac ctcattttga cgctcaacca ataaatggga ctgtttatct ttttcgttct
     3661 tttctacagc aagtccatat atttgttttt gatagtcaac aagccgctct tccatggagt
     3721 taaccacatc catgttttct tcgagcgagg cgttaatctc gtctatctct gcctgtaccg
     3781 tggaacgctt tgtctgtata tccgagatat cctgacttcg tctgtccttg tcttgcgtaa
     3841 aacccttaag tcgcaacaat tgaatatcaa gctcattttc aaatattgct tcgcgcaatg
     3901 tacgatactt tacggtttta tccgcctgaa ttttaagggt gtcgtacgat cgcttaacct
     3961 cgccaagtat cccttcaatc tgtcgcatgt tttcttcggt tttggaaagc tttcgctctg
     4021 cctcggctcc gcgaacctta aaccgggtaa tccctgccgc ttcttcaaaa atgtaccggc
     4081 gctcttcggg tttactagaa agaatctgat caatctttcc ctgctccatg actgaatagg
     4141 cggccttacc aacaccggta tcccaaaata gttcgcgaat atctttgagc tttaccgggg
     4201 tattgttgat gtagtattcg ctttctccag aacgatacaa gcggcgctta atggcgatct
     4261 cactgacatc cagatttaac agtccagcct cattcgcaat agtcagcgtt acctcggcaa
     4321 cattgagcgg cttccgcgat tccgttccgt taaagataac gtcttccatt ttgtcggctc
     4381 gcatagcctt ggaggcctgt tcaccgagta cccattttac cgcatcgacg acattactct
     4441 ttccacagcc atttggaccg agcaaggcgg ttatgccgtc ggaaaactgg atatgggttc
     4501 tgtcggcgaa ggatttaaaa ccgaatatat caagactttt aagaaacacg ttatttgggc
     4561 cccttggtta aaaactgacg aaaaatcagg gtatactgta ctctagaggg catactccgg
     4621 tcaactccgt tcaggtggca cttcttcatc aatatctcca ttatgtctac tgtcactgct
     4681 ttatctttag acttgtcatg agagagccga tagagaaatc atggctgaaa cactggtact
     4741 tttctcgtcg gatactcccg gcgaaagcca gatatgccgt tcactgcgta gggccggata
     4801 caccgtgaaa agggtgcaca ccctttctgc tctgcatacg attgcgtatt ccttcacggt
     4861 tttagcggtt gtccttaacc gcacaatcat cgagcgatac tgtctggaac caatcaaaca
     4921 tctcaaacga gcgagaagcg catgcgccat cattagctgg cattcgggat tgtatcatcc
     4981 agaggtcgac agcgtaagtg tcttgtgtga atactccacc aatggctgga cccaagagcg
     5041 acggatagaa gaactcgacc acgtggtaaa aatcatagaa aaccccgaaa aagatgaggt
     5101 ccagggggac ttatttgtta ctgatgaaac aacccgatat ggaaaaagag gacttgttac
     5161 agaaacagat attcccttgg agaaaacgca taaaaaaatg cgcaaggtat atgaattact
     5221 ggtggatgca ggctcaaaag gaatcagcgt tgaagattta tcaatacgat tatggggaag
     5281 aaatgcacag atcaaacggg acgaccttca aagctatatg agcaaactgc gcaaattact
     5341 aactgaggcc tctggtggaa agcgaacgat tgcaagaaaa ggagaaacct accgtattag
     5401 ccccatacac aacgaaacgg cagagcaata aaaaaggagg gcgtatcagc cctcctgtgc
     5461 actcttattt accaagtacg gtagaaattc gctcaagcac cttcttgcga tcaagtggct
     5521 tgacgatata atttttagct ccaaggagaa gcgctttttt tacgagctct tccttaccaa
     5581 gggcgctaat cattacaatc ttggcatttt tatcaaaggc cattatctgc tcaagggcgg
     5641 taattccatc catcttgggc atggttatat ccatggttac caaatcgata ttcggacaga
     5701 gttccttata tttctcaact ccatcaagac catcggttgc agtggtaaca atctcgtagc
     5761 cttcactggt caaaatctga ccaatttgtt tggcgacgaa catagaatcg tctacaacca
     5821 gtacgcggta cggtgtgccg tccgccttta aaccctcggg gggccgttca ttgatcgccg
     5881 gaaaatcttg tttagatatc atacactcac tccccttctt tacatgcggt cacgtatcgc
     5941 tacgtttact tctaccttac cctgatctgt ttccattgga acaatcagag cctctacttc
     6001 atgatcagaa atctccatat tgtctccggt aaacagagcc ggaggcgtta aatcaaaacg
     6061 gaaacccaga tcgtgcagtt ttgttacggc ctgtgcggta atcaaattgg caagttcggt
     6121 tatagtcgcc tttgccaaat cgtcaaaggt ctgtagttgc tcgccgttca tttgggaagc
     6181 aatcttcatc gctgaatcca agctcatgtc aaagataact cgaccttcaa catcacctgc
     6241 caaaccaaca agggcagcca cacccataac aggcatggac gttgatttca gatacaattc
     6301 acctcgtttg atctcgccac ccaacacctc tgtcaagatg ttatacgcag cttccacaaa
     6361 cggattaata tactctacac gcattggtct ctccttctat tatgatgctc aatcactctt
     6421 tgctgaaagc cacaatatct ccgcgaacgg ctcgcaacca tccgctatg
//
LOCUS       DAOQXQ010000012         6230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_12898_length_6230_cov_3.739919, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000012 DAOQXQ010000000
VERSION     DAOQXQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_6230_cov_3.739919"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PKH81_00410"
     CDS             complement(<1..1234)
                     /locus_tag="PKH81_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ96538.1"
                     /translation="MTSASYSANNITVLKGLEAVRKRPGMYIGSTGPDGLHHLVYEVV
                     DNCIDEAMAGQCDRILVVLEKNDIVRVEDNGRGIPVDIHPAEGVSALELVLTRLHAGG
                     KFDKGSYKVSGGLHGVGVSVVNALSVWMEAFVSRDGYDHYQKFEIGVPTEPVKRLGAT
                     DNCGTTIRWLVDSSIFTETTTYNFDVLATRLRELAFLNSGITIVLRDERLTNPKELEF
                     RFEGGIRSFVSYLNENKTCLHHEPVFITGERDDVIVEIAFQYNDTYSEVMLSFVNDIN
                     TREGGTHLVGFKSALTRVLNEFLKNSKLAKKMEETLSGDDVREGLTAVLSVKVPEPQF
                     EGQTKTKLGNSEVRGIVESFVNDQLTLYLEQNPNVISSILEKSVLAAKARIAARQARD
                     ATRRKNAMDSSGLPGKLAD"
     gene            1321..2727
                     /gene="dnaA"
                     /locus_tag="PKH81_00415"
     CDS             1321..2727
                     /gene="dnaA"
                     /locus_tag="PKH81_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ96539.1"
                     /translation="MGNWDYEIFWKESLNQLKAELSEQEFAMWFNMAYETSGELSLVV
                     SVPSAFYRDQVKQRYQERIEHTLFELSGQHIQVEFEIKQRKHPDHKEIVSSHVSPASI
                     DRPTQSSSHKKTEGTVQQIKDREDHGQLRRDYTFENFVIGENNSFAANAAIAIAKNPG
                     TAYNPCLIYGGVGLGKTHLMQAIGNLAWVEKGCKIIYITAENFTNEFVEAIKNSTTQK
                     FKNKYRNADMLLIDDIHFLQKKNETQEELFHTFNALYDANKQIIFTCDRPVSELKNLS
                     ERLRSRFERGLNVDLQPPSYETRCAILSKKVEARKVHIPADVINLVAKNISSNVRDLE
                     ASLTKLIAYAELTKKPVTLETAQQQLRDTFGSPKQNNVTIETIQKVVAEYFSLSYTDL
                     KGKKRTKAVSFPRQIAMYIAREITEYSTTELGMEFGGRDHTTVMHGCQKIEERLKMEP
                     SLETTILKLEKLIKDYNS"
     gene            2970..4073
                     /gene="dnaN"
                     /locus_tag="PKH81_00420"
     CDS             2970..4073
                     /gene="dnaN"
                     /locus_tag="PKH81_00420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ96540.1"
                     /translation="MKFSFDRDALLKEISIAQEIIATKNAISILSNVLLEASDGTLCI
                     KATDIKVNFETRIPVNVLESGSTTVFCDKFMSIISSLPSGEIEFEQNDIKITIRSITK
                     KARFQLKSITSDKYPEFTAPADIAFFDIPVKEFKEMIYQTVFSVSDDETRYFMNGVFM
                     EKQADKLQLVATDGRRLAFIAKEFATPLPDFKGVIIPPKILNIINKRASEEGPLSVGV
                     GEKNIFFRFGNYEFSSVLIDGQFPNYSRVIPESQAYSFEVETKDIVEALKRVALLVEQ
                     KSRRVYLAIAPGKLTISSQETELGTANEEIPCKYDGNEMTIALNYLYVEEPLKVIGTD
                     RVKFEFSEAMKAVTIKAEPAKDFFHIVMPMQME"
     gene            4086..5168
                     /gene="recF"
                     /locus_tag="PKH81_00425"
     CDS             4086..5168
                     /gene="recF"
                     /locus_tag="PKH81_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757183.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNQ96541.1"
                     /translation="MPFLSISFTDFRNVQNETLDLLAREVYFIGDNGQGKSNLLESMY
                     LVSYGGSFRTRDDSELVRNGQQAYSVRALYKNQAERTSTVSVVYKEKKKRIEKNAKVI
                     LDRKELIDTIPCILYNHDDLDFASGSPERKRFFIDQSLSMYDSSYIDVLRKYKKILKT
                     RNQVLKDKRADILESIDIQLVEIGLEVCKRRKKTIIDFNRVFSALYEEVSGLRDVHIQ
                     YGPSWKTDTVEQVLGEVFARRALDMSFGMTSSGPHRDRIRFMKGSIGFIPTASTGQRR
                     LISLLLRTAQASYYTEVSGKLPVLLMDDVLLELDPEKRQRFTALLPEYDQLFCTFLPG
                     EPFERYKKTTTKVYDVSEGGWNERIH"
     gene            5152..5616
                     /locus_tag="PKH81_00430"
     CDS             5152..5616
                     /locus_tag="PKH81_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNQ96542.1"
                     /translation="MSASIKKASELITSLFENLSCEDLKNAQNFTRSWKAAVGDKIAS
                     HSKIIDVNKGTIIVEVDHPGWSQHLLLMKQRLLRELAGSFPELEIANLAIRIKQHCPT
                     PYVRQDGIVGEGVFREDATTEEADVPIREDFDSDLKDVLARLKSSIKKGKPS"
     gene            5750..5905
                     /gene="rpmH"
                     /locus_tag="PKH81_00435"
     CDS             5750..5905
                     /gene="rpmH"
                     /locus_tag="PKH81_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ96543.1"
                     /translation="MKRTYQPSKVKRNRKFGFRARMATRGGRLVLKRRRAKGRYKLSV
                     SDEKKPY"
     gene            5926..>6230
                     /gene="rnpA"
                     /locus_tag="PKH81_00440"
     CDS             5926..>6230
                     /gene="rnpA"
                     /locus_tag="PKH81_00440"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ96544.1"
                     /translation="MKRSGFTKAERIKSSAAIQTVFRKGKKYSCDGAKLFVMANGMGW
                     NRVVFTFPRGYGSAVERNHSRRLSREVYRVLKARLARGYDLTILVFPGKDSYAVREQ"
BASE COUNT         2018 a         1170 c         1269 g         1773 t
ORIGIN      
        1 aatcagcaag ttttcccgga agcccggaac tgtccatggc gttttttcgg cgtgttgcgt
       61 ctcgagcctg tctggcagca atacgtgcct ttgcagccaa aacgcttttt tccagaatgg
      121 aacttattac attaggattc tgttctaaat ataaagtaag ttgatcgtta acaaagctct
      181 caactatacc gcgaacttcg gaattaccaa gttttgtttt tgtttggcct tcaaattgtg
      241 gttcaggaac cttaaccgaa agaactgcgg ttaacccttc gcgtacgtca tccccggaaa
      301 gagtttcttc catctttttg gcgagcttag aatttttgag aaattcattt aataccctgg
      361 ttaatgcact cttaaacccg acaaggtgcg ttccaccttc gcgtgtatta atgtcgttaa
      421 cgaaactaag catgacctcg ctataagtat cgttatattg aaaggcaatt tctacgataa
      481 catcatcgcg ctcccctgta ataaacaccg gttcgtgatg cagacaggtt ttattttcgt
      541 ttaaataact gacaaaactg cgaattccac cttcaaagcg gaattccagc tctttggggt
      601 ttgttagtcg ttcatcgcga agtactatgg tgataccgga attcaagaag gcaagctcgc
      661 gaaggcgtgt tgcaagaaca tcaaagttat acgttgttgt ttctgtaaaa atagagctat
      721 caaccagcca gcggatcgtt gttccgcaat tatccgtggc tcctaaccgt ttaactggct
      781 ccgtgggtac accaatctcg aatttttgat agtgatcata accatcccga gaaacaaatg
      841 cttccatcca cacagaaagc gcgtttacaa cggaaacacc aactccatgg agcccgccag
      901 aaactttgta tgatccttta tcaaatttac cgccagcatg aagccgggtt agtacaagtt
      961 caagcgcgct taccccttca gctggatgaa tatcaaccgg aattccacgg ccattatctt
     1021 ctactcgaac tatgtcgttt ttttctaaaa caaccaaaat tcggtcacac tgaccagcca
     1081 ttgcctcgtc aatacagtta tcgacaactt catacacaag gtgatgaagt ccatccggac
     1141 ctgttgatcc gatatacata ccaggtcttt tgcgaacagc ttcaagaccc ttgagaacag
     1201 taatattatt agcggaataa gaggcggaag tcattctgta tccttatatg gtagaaacac
     1261 gtatttttcc ataataccgt cattgtcaaa aaaagtaaag aaggggtata atggccgcct
     1321 atgggaaact gggattacga aattttctgg aaagagtctc tgaatcaact aaaagctgaa
     1381 ctatcagagc aagaattcgc catgtggttt aacatggcat acgaaacctc gggagagcta
     1441 tctttggtag tttcggttcc ctcggctttt tatcgagatc aggtaaagca acgatatcaa
     1501 gaacgtattg aacatacctt gtttgagcta tcaggacagc atatccaggt agaatttgaa
     1561 atcaagcaaa gaaaacatcc cgatcacaaa gaaattgtat caagtcatgt ttctccagcc
     1621 tctattgatc gaccaactca gagctcttcc cataaaaaaa ctgagggaac agttcagcag
     1681 ataaaagatc gtgaagatca tggtcaacta cgtagagatt ataccttcga aaattttgtg
     1741 ataggagaaa acaactcctt tgctgcaaat gccgccattg ctattgcaaa aaatcctgga
     1801 acagcataca acccctgtct tatttatggt ggggtcggct taggtaagac acacctaatg
     1861 caggcaattg gaaatcttgc ctgggtagaa aaagggtgca agattatcta tataacagct
     1921 gaaaacttta ccaatgagtt tgtagaggcc atcaagaact caacaacaca aaaattcaag
     1981 aacaaatatc gaaatgcaga catgctgcta attgatgata ttcacttttt acaaaaaaag
     2041 aatgaaacac aagaagaatt atttcatacg tttaacgcac tctatgacgc caataaacaa
     2101 attattttta cctgtgatag accagtgtca gaattaaaaa atctatccga acgattacgt
     2161 tcccgctttg aacgaggatt aaatgttgat ttgcaacccc ctagttatga gacccgctgt
     2221 gcaatccttt caaaaaaagt agaagctcga aaggtccata taccggcgga tgttattaat
     2281 ctggttgcca aaaacatttc ctcaaatgtt cgagacctgg aagcatctct tacaaaactc
     2341 atcgcttacg ctgaattaac aaaaaaacct gttacccttg aaactgctca acaacaattg
     2401 agggacacct ttggttctcc caaacaaaac aatgttacca tcgaaaccat acaaaaagtt
     2461 gttgcagagt atttttcgct atcttataca gacctaaaag ggaaaaagag aacaaaagct
     2521 gtatccttcc ctcgtcaaat agctatgtat attgcgcggg aaataactga atattcaact
     2581 acggaattag gaatggaatt tggtggtaga gatcacacaa cggttatgca tgggtgccaa
     2641 aaaatagaag aaagacttaa aatggaacct tcgttggaaa ccacaatcct taaattagaa
     2701 aagttaataa aggattacaa tagttaatta agtgatatat tcttgttttt cttcttgttg
     2761 atactatgat agaatatggt gtactttctg tggacaaaac ctgtccctgt gtatatgtgg
     2821 atatcagtgt ctggtaaaat gaccagttat tgattgcgta attctttata gagcaaagaa
     2881 atttaccggt tatccggaaa tccaccggct ctattactac tactactatt ttatatatat
     2941 ataactagta agatagattc ggaggaacta tgaaatttag ctttgacaga gatgctttat
     3001 taaaagaaat ttccattgca caggaaatta tcgcaactaa aaacgcaatc tcaatacttt
     3061 caaatgtatt gcttgaagcc tccgacggaa ccttgtgcat taaggcaacg gatatcaaag
     3121 ttaattttga aacccgtatt cctgttaatg ttctagaaag tggttcaact actgtttttt
     3181 gtgataaatt catgagtata atttcatccc taccttctgg ggaaattgaa tttgaacaaa
     3241 acgatataaa gataacgatt agatcaataa caaaaaaagc tcgctttcaa ctaaaaagca
     3301 tcacgagtga caaatatccg gaatttactg cccctgccga catagccttt tttgatattc
     3361 ctgttaaaga atttaaagaa atgatatatc agacagtatt ttcggtatct gatgatgaaa
     3421 ccaggtattt catgaatggt gttttcatgg agaaacaagc agacaaatta cagttagtag
     3481 caactgatgg gcgaagactg gcttttattg caaaggaatt tgctacaccc ttaccggact
     3541 ttaagggagt aataattccc ccaaagatac ttaatattat taataaaaga gctagtgaag
     3601 aaggacctct ttctgtaggc gtaggagaaa aaaatatttt ctttcgattt ggaaattatg
     3661 aattttcctc tgtactaatt gatggacagt ttccaaatta ttcaagagta attccggagt
     3721 ctcaagctta ttcttttgaa gttgaaacca aggatattgt tgaagcctta aagcgcgttg
     3781 ctttacttgt agagcaaaag tcacgaagag tatatttggc tattgcacca ggtaaactta
     3841 ctataagttc tcaagaaact gagttgggaa ccgcaaatga ggaaattcct tgtaaatacg
     3901 atggaaatga aatgaccatt gctttgaatt atttatatgt tgaagaacct cttaaagtga
     3961 ttggtacaga tcgtgttaag tttgaattta gcgaggctat gaaggctgta accatcaagg
     4021 ctgaaccagc taaagatttt ttccatatcg taatgcctat gcagatggaa taagtattta
     4081 agtccgtgcc gtttctttca atttccttta ctgattttcg caatgttcaa aatgaaacat
     4141 tagacctcct ggccagggag gtctatttta tcggggataa tgggcaaggt aaaagcaatt
     4201 tacttgaaag tatgtatctt gtttcctatg gtggatcatt tagaacaaga gatgatagcg
     4261 agcttgttcg taatggccaa caagcatata gtgttagagc cttatataaa aaccaagcag
     4321 aacgtaccag cacagtatct gttgtttata aagagaaaaa gaaaagaata gaaaaaaatg
     4381 caaaagtgat tcttgatcgt aaggaattga tagatactat tccatgtata ttatacaacc
     4441 atgatgatct ggattttgct tctggttctc cagagagaaa gcgttttttt atagatcaat
     4501 cattatccat gtatgacagt tcgtatattg atgtactacg gaagtacaaa aaaattctta
     4561 aaaccagaaa tcaagtatta aaagataaaa gggcggatat tcttgaatca atcgatattc
     4621 aactggtaga aattggcctt gaagtctgca aacgaagaaa aaaaacaata attgatttta
     4681 atcgtgtttt ttcagccttg tatgaagaag tttctggtct tcgggatgtc catattcaat
     4741 atggaccttc atggaaaaca gacacagtag aacaagtact tggagaagta tttgctagac
     4801 gtgcccttga tatgtccttt gggatgactt cttctggacc tcacagagac agaatacggt
     4861 ttatgaaggg ttcaataggg tttattccta ctgcatccac aggacagcga agattgatat
     4921 cgttactgct tcgaactgcg caagcctcat attatacaga ggtatcaggg aaactaccag
     4981 ttcttttgat ggatgatgtt cttcttgaat tggatccaga aaaaagacaa cgatttacag
     5041 ccttattacc agaatatgat cagctttttt gtactttttt gcccggagaa ccctttgaaa
     5101 ggtataaaaa aacgactacc aaagtatatg atgtgtcgga agggggttgg aatgagcgca
     5161 tccattaaaa aagcatcgga attgattaca tcgctctttg aaaatctcag ttgtgaggat
     5221 ttaaaaaatg cccaaaattt taccagaagt tggaaagccg ccgtcggaga taaaattgca
     5281 agtcatagca aaataatcga tgtaaacaag ggtacgatca ttgtagaggt agatcatcca
     5341 ggttggagcc aacacctttt attaatgaaa caacgattat tgcgtgaatt agctggttct
     5401 tttccggaac tggaaatagc gaatcttgca ataaggatta aacaacattg ccccaccccc
     5461 tatgttcgtc aagatggaat cgtaggcgaa ggggtgttta gggaagacgc tactacagaa
     5521 gaggcggatg taccaattcg agaagatttt gatagtgatt taaaagatgt cctagcacgt
     5581 cttaagagta gtattaagaa aggaaaacct tcctgagggc tcttgccctg tcttgacctg
     5641 taaagaggcg taatgtatac tgccatgcct gactagcagg attagagcac tacaaaccct
     5701 gaagtgaaac atagctttcg ggtttacaaa taaaggagtc tagagttcca tgaaacggac
     5761 atatcagccc agtaaggtta agcgcaacag aaaattcggt tttcgcgcac gtatggcaac
     5821 ccgcggagga cgtttggtcc ttaagcgtcg tagagcaaag ggtcgttata agttaagtgt
     5881 ttctgacgag aaaaagccgt actgatctac aaagtgaatt gagccgtgaa acgctcaggt
     5941 tttaccaagg cagagcgaat aaaaagctct gccgcaattc agactgtttt caggaaggga
     6001 aagaaatata gctgtgacgg agcaaaactc tttgttatgg ctaacgggat ggggtggaat
     6061 cgggtcgtat ttacctttcc acgaggatac ggttctgccg ttgagagaaa ccattctcgg
     6121 cgcctgagcc gcgaggtgta tcgtgtgcta aaagcgcgac tagcgcgcgg ttatgatctg
     6181 acgattttgg tttttcccgg aaaagattcg tatgctgtta gagaacagca
//
LOCUS       DAOQXQ010000013         6218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_12931_length_6218_cov_2.708583, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000013 DAOQXQ010000000
VERSION     DAOQXQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12931_length_6218_cov_2.708583"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKH81_00445"
     CDS             complement(<1..506)
                     /locus_tag="PKH81_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ96545.1"
                     /translation="MASVWIDGTWFAKDEAKISVYDHGLLYGDGLFEGIRMYGGQIFM
                     LESHLDRLFDGARAISLHIPYTREELSLMLKEGVRREKRDDGYIRLIVTRGKGDLGIN
                     PKTCPRATVIAIIDSISLYPSEYYEKGIDIVTASSRRLQGDIFDPRIKSLNYLNNVLA
                     KLEAQVAG"
     gene            complement(487..1332)
                     /locus_tag="PKH81_00450"
     CDS             complement(487..1332)
                     /locus_tag="PKH81_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ96546.1"
                     /translation="MVDNVVNRLMELGLSEYESRAWLSLVRSHPRTAYETARESGIPT
                     AKIYMVLDRLVERGFVLEISTADKKRYVPCEIEDFLSGFRLKTEKIISSLEIDLERIR
                     EDKTPVSYVWNLEGRTEILSRAETMIRGATSTILISVWKEEYISLLPVLQEKETQGLK
                     IAIVFFGDRGDVPVCGSQYQHPIADTLYQERGGRGFALVTDGKTACTATIPDKGHSGS
                     EQGAWSRSTGFVLLAEDYIKHDIYIMKIVSRFDEELSCRFGERYRLLRDIYKDKEVQD
                     GVGMD"
     gene            1397..1867
                     /locus_tag="PKH81_00455"
     CDS             1397..1867
                     /locus_tag="PKH81_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96547.1"
                     /translation="MNIGIIVWSATGHTESVAQNMKETLEQQGHSVSFSKIAIDVDPL
                     KRADTYSIIEAPDSSPYDALILGAPVEAFCLSDVMKRYLTHQPALTGKKAFYFVTQYF
                     PKPWMGGNNSVKQIQKLAESKGAISVGKAVINWSSSSRQTQIDKAVASVMSAFS"
     gene            1910..2416
                     /locus_tag="PKH81_00460"
     CDS             1910..2416
                     /locus_tag="PKH81_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HNQ96548.1"
                     /translation="MKHTPTLIAFVLLSLICLSVNAQDKSWEGNGFVVTWKLSTDQLE
                     MTIETETTGWVSVGFEPTRIMKDANIIIASVLPDGSVVAEDHYGTGTFSHKKDTDLGG
                     TKNVTVTSGSESEGKTRITFSIPLNSGDSKDVILTPGKKTKIILASSKADSFTKKHNK
                     KAKTEIEL"
     gene            2413..2637
                     /locus_tag="PKH81_00465"
     CDS             2413..2637
                     /locus_tag="PKH81_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96549.1"
                     /translation="MKAQLKQKLLVPINIMLFICFVIQMSTALAFVLGFAGSLSYFLH
                     MNVGFLMGLLVICHVYLNWSWIAAKLFKAS"
     gene            complement(2645..3043)
                     /locus_tag="PKH81_00470"
     CDS             complement(2645..3043)
                     /locus_tag="PKH81_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96550.1"
                     /translation="MNKAVGVMVLMFLCCALVCAGGSADSYSEDGYIRAVARDFGRTL
                     EEAEKSVASATWDWYEKKWEGEWTEERFKNAVTQGVENCKNGAMITAAKAGKTGKKVL
                     KALVVAAGDAVEAVSGWVDKNSERYDEERK"
     gene            complement(3151..4620)
                     /gene="ilvC"
                     /locus_tag="PKH81_00475"
     CDS             complement(3151..4620)
                     /gene="ilvC"
                     /locus_tag="PKH81_00475"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNQ96551.1"
                     /translation="MNYFNSIPWRAALMQLGTCRFMDRNEFSDGTAALKGKKIVIVGC
                     GAQGLNQGLNLRDSGLDVSYALRKEAIAEKRASWKNATENGFTVGTYEELIPNADVVG
                     NLTPDKQHTPVITAVMPLMKKGSALWYSHGFNIVEEGMQIRKDITVVMMAPKGPGSEV
                     RVEYSRGFGIPTLIAVHPENDPEGKGWAIAKALAVGTGGSRAGVLESSFIAEVKSDLM
                     GEQTILCGMLQTGSLLCFDKMVASGIDKGWASKFIQYGWETITEALKWGGITGMMDRL
                     SNPAKLKAQALSVELKKIMGDLYRKHQDDIISGKFSTTMMEDWKAGDKDLLAWREATG
                     KTAFEQTPAGNVEITEQEYFDKGILMVAMVKAGVELAFETMTDAGIKAESAYYESLHE
                     TPLIANLISRKKLYEMNKVISDTAEYGCYLFDNACRPLLKDFMTRVELDVIGKGLTVK
                     DSRVSNRELVTVNEEIRNHPVEKIGKVLRSYMTAMKEVI"
     gene            4750..5724
                     /locus_tag="PKH81_00480"
     CDS             4750..5724
                     /locus_tag="PKH81_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ96552.1"
                     /translation="MDLFELEAFLVLSEQLHFARAASRVNLSPSALSRLLSRLEDETG
                     VILLDRDTRQVQLTRSGEEFRDFARETILRRKELSLRLTQTDNRLRGTLRVYASVTAC
                     YSILPPFVEALSREHPELSLSVETGDPAGAANAVREGRADIAVSSTPAEGYTDLSSFT
                     VNKTPLVFAASQKGLYSHLSLPRDTKNSTGNNLVEEKSLIETLLSVPLLLPKAGLARA
                     RFDRWMEELQQPEHKGKKPQIAIETAGNEALLALARLGLGLALVPRLVLENSPFAEGL
                     VLYQAGPHFGDYDIGFVMKSIDRAPRGARHLTEALFLLIKKTYPIHTI"
     gene            5764..>6218
                     /locus_tag="PKH81_00485"
     CDS             5764..>6218
                     /locus_tag="PKH81_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96553.1"
                     /translation="MKSPLEHPQELITISNCSINNQETTILSHLSLIIKAGHNWLITG
                     SNGSGKSALGAALCQSKDLSIIPHDGGLYSNTLSSFISRVSFELAASLIEDEHKRDDS
                     DFIEGGIDSGRTPRNLLDNPSNQNPYVMLTGIEAFLDTGLKFLSTGEIR"
BASE COUNT         1749 a         1586 c         1129 g         1754 t
ORIGIN      
        1 caaccggcaa cctgagcttc caacttggct aacacattat ttaaataatt caaagattta
       61 attctgggat caaaaatatc tccctgtaat cttcgcgaag acgccgtaac gatatcaata
      121 cctttctcat aatattcaga tggatacagg gatatcgaat caataatcgc aataacagtt
      181 gcacgagggc acgtttttgg atttatacca agatctccct ttcccctcgt aacaatcaac
      241 cgaatatatc cgtcatcccg tttctcacga cgaacccctt ccttaagcat caatgaaagt
      301 tcttctcgtg tatacggaat atgtaatgat attgcacgtg caccatcaaa caagcgatct
      361 aagtgggact ccagcataaa aatctgccca ccatacatcc gaataccctc aaaaagacca
      421 tccccgtaca ataaaccatg gtcatacacc gatattttcg cctcatcttt ggcgaaccag
      481 gttccatcaa tccataccga cgccatcttg aacctccttg tccttataaa tatctcttag
      541 caaccgatac ctctcgccaa agcggcaaga cagctcttca tcaaagcgag agactatctt
      601 cattatgtag atgtcatgct taatgtaatc ctcggcaagt aacacaaaac cggtgcttct
      661 agaccaagcc ccttgttcag agccactatg tcctttgtcg ggtattgttg cggtacaagc
      721 agtttttcca tcagtaacta aagcaaaccc ccgacctccc cgttcttgat acaaggtgtc
      781 agcgatagga tgttgatact gcgaaccaca gacagggaca tctcccctat ccccaaaaaa
      841 cacaatagct atcttaagtc cttgcgtttc cttttcctgc aaaaccggaa gtaacgagat
      901 gtattcttct ttccaaacag atatcaaaat tgtagaggta gcccctcgta tcattgtctc
      961 tgcccggctc aaaatctccg ttctgccttc cagattccat acatacgaaa caggggtttt
     1021 atcttcccta atccgttcaa gatctatttc cagggaagaa ataatcttct ctgtttttaa
     1081 cctgaatccc gataaaaaat cttcaatttc acacggtaca tacctttttt tatcggcggt
     1141 tgagatttcc agcacaaaac ctcgttctac caaacggtct aacaccatat aaatctttgc
     1201 cgttggaatt ccactctctc ttgccgtttc ataggcggta cggggatgag agcgaacaag
     1261 agacaaccac gccctgcttt cgtactccga gagccctaat tccatcaatc gattgactac
     1321 gttatctacc atggtagtca ccatacatca tggaaatact acttgtcaat tcataaaacc
     1381 gtcttatact gattttatga atataggaat aatcgtctgg tcagctacag gtcatactga
     1441 atccgttgca caaaacatga aagagactct tgagcaacag gggcattctg tttcattttc
     1501 caaaatagcc atagacgttg atccattaaa acgggcggat acgtattcaa taatcgaggc
     1561 ccctgactcc tcgccctatg acgcccttat acttggggcg ccggtagagg cattttgcct
     1621 ttccgatgtc atgaagcggt atcttacaca tcaaccggcc ttaacaggaa aaaaggcctt
     1681 ttatttcgta acccagtact ttccaaaacc ctggatgggt ggaaacaatt cggttaagca
     1741 aattcaaaaa cttgctgaat caaaaggggc catttctgtc gggaaggctg ttatcaattg
     1801 gagctcctcc tcccgacaaa cgcaaattga taaggcagtt gcctcggtta tgtccgcttt
     1861 ctcttaaaaa cacttgccgg acagccattc taggcctaca cttcaacata tgaaacacac
     1921 tccaacatta atcgcgtttg tcttgctttc actcatttgt ctatccgtaa acgcccagga
     1981 caagagctgg gaaggaaatg gatttgttgt tacatggaag ctatctaccg atcaactgga
     2041 aatgacgata gaaactgaga caacgggctg ggtatctgta ggctttgagc ctaccaggat
     2101 tatgaaggat gcaaacatta ttatcgcttc agtattgcct gatggatctg ttgttgccga
     2161 agatcactat ggaacaggga ctttttcaca caaaaaagat actgaccttg gcggtaccaa
     2221 gaatgtaacg gtcacaagcg gttctgagtc agaaggaaaa acccggatta ccttttcaat
     2281 tccgcttaat tccggggaca gtaaggatgt gatcctgacc ccgggcaaaa aaaccaaaat
     2341 catccttgcc tcaagcaaag cagacagttt taccaaaaag cataacaaaa aggctaaaac
     2401 ggagattgaa ttatgaaagc gcagttaaaa caaaaattat tagtaccaat taacattatg
     2461 ctctttatct gctttgtaat tcaaatgagt acagcgctgg cttttgtcct gggctttgca
     2521 ggtagccttt cttatttttt acatatgaac gttggttttc taatgggtct attagtaatc
     2581 tgccacgtat atcttaactg gtcttggatc gccgcaaagc tcttcaaggc cagctagacc
     2641 ccttttattt tcgttcctca tcatagcgtt cgctgttctt atcaacccag cccgagaccg
     2701 cttcaacagc atcacctgct gctaccacca aggcttttag aacttttttt cctgtcttac
     2761 ctgccttggc agcggtaatc attgctccat ttttacaatt ttccacaccc tgagttactg
     2821 catttttaaa tcgttcctca gtccactcgc cttcccattt tttttcatac cagtcccaag
     2881 tagcactcgc taccgatttc tctgcttctt ccaaggttcg tccaaaatcc cttgctacag
     2941 ctcgtatata accatcctcc gaataggaat ctgctgaacc tccggcacat accagggcac
     3001 agcacaaaaa catcaatacc ataacaccaa cagctttatt cattcggtcc tccatataac
     3061 tcgtaacata aaaaaaatac cgaatcaaga ctcttgtgct tcttgatccg gtatcgtaag
     3121 tcattctata tacaacaata accctgtgtt ttaaatcact tccttcattg ctgtcatata
     3181 agaacgaagg accttaccta ttttctcaac tggatgattg cgtatttctt catttaccgt
     3241 aaccagttcc cggttggata cacgactatc cttaaccgtt aaacctttac cgataacatc
     3301 aagctccacg cgtgtcataa aatcctttag gagaggacgg cacgcattat caaaaaggta
     3361 acagccatac tctgcggtat cagagataac cttgttcatc tcatacagtt ttttccggct
     3421 tattaaattg gcaataagtg gcgtttcgtg aaggctttcg taatatgcgc tttcagcctt
     3481 aattccggcg tctgtcatgg tttcaaacgc aagctcaaca ccagccttta ccatcgcaac
     3541 catcaaaata cccttgtcaa aatattcctg ttcggtaatt tctacgtttc cggcaggagt
     3601 ttgttcaaag gcagttttac ctgttgcttc gcgccatgct aaaagatcct tatctcccgc
     3661 tttccagtct tccatcatgg tagtgctaaa ctttccagag ataatatcat cttgatgctt
     3721 gcggtacaaa tcgcccatta tcttcttgag ttcaacagac agggcctgtg cctttagctt
     3781 ggcaggattt gaaagcctgt ccatcattcc tgttataccg ccccacttaa gggcttcggt
     3841 aatggtttcc catccatatt gaataaattt agacgcccag cccttatcaa tcccggaagc
     3901 aaccatcttg tcaaaacaca aaaggcttcc agtttgaagc attccacaaa ggatggtttg
     3961 ctcacccatt aaatcgcttt ttacctctgc aataaagctc gattcaagaa cacctgcacg
     4021 gcttcctccg gtaccaacgg caagagcctt agcaattgcc caaccttttc cttctggatc
     4081 attttctgga tgaaccgcga taagggttgg aataccgaac ccgcgggagt attctacacg
     4141 aacttctgaa cccggacctt tgggagccat cataaccacg gtaatatcct tacggatttg
     4201 cataccttct tctacaatgt taaatccatg gctgtaccac aatgcagagc ctttcttcat
     4261 aagaggcatt accgctgtga taaccggtgt gtgctgttta tccggggtca agtttcccac
     4321 aacatctgca tttgggatta attcttcata ggtcccaaca gtaaaaccgt tttcggtcgc
     4381 gtttttccaa ctcgcccttt tttcagcaat agcttctttt cgaagcgcat acgatacatc
     4441 aagtcctgaa tcgcgaaggt tgagtccctg attaagacct tgtgcaccac aaccaacaat
     4501 cacgatcttt tttcccttca gggctgcagt tccatcagaa aactcattcc ggtccataaa
     4561 acgacaggtt cccagctgca taagtgccgc acgccaggga atagaattaa aataattcat
     4621 actaataact cctcatacat agacaataaa aaaatagtac ttatgtatac ctctttcttt
     4681 tagatgcgta aagcgtaaca tttgcacggc actattgcgt ttcgcgcatt ataactatac
     4741 agtgtctcca tggatctttt tgaactagaa gcttttctgg tgttaagcga acagcttcat
     4801 tttgcacgtg cagcgtctcg cgtaaacctg agcccttccg cactcagtcg tcttttgtct
     4861 cgtctggagg atgaaactgg cgttatcctt ttggatcggg atactcgaca agtacaatta
     4921 acccggtccg gagaggaatt cagagacttc gcaagggaaa ctatccttag aagaaaagaa
     4981 ctctctctcc ggctaacaca aacagataat cgcctccgtg gtaccctgcg ggtctatgca
     5041 tcggttaccg cttgctattc aattcttcca ccctttgtag aagccctgtc tcgtgaacac
     5101 ccggaattga gcctctcggt agaaaccgga gatcccgcag gtgctgcaaa cgcagttcga
     5161 gaaggaaggg ctgatatcgc cgtttcatct accccggcag aaggatacac cgatttatcc
     5221 tcttttacgg taaataaaac acctcttgtt tttgccgctt cccaaaaagg tctatattca
     5281 catctttcct taccaaggga taccaaaaat tcaacgggaa acaaccttgt agaggaaaaa
     5341 tcgctaatag aaacactctt atctgtcccg ctcttattac ccaaggccgg cctggctcga
     5401 gcacgatttg atcggtggat ggaagaacta caacaaccgg agcacaaggg taaaaaaccc
     5461 caaatagcaa tagaaacagc aggaaatgaa gcccttctcg cccttgcccg ccttggtctg
     5521 gggctcgcac ttgttccacg cctggtactg gaaaacagtc cttttgccga aggattagtg
     5581 ttatatcagg caggcccaca ttttggcgat tatgacatag gattcgtcat gaaatccata
     5641 gatcgcgcac cacgtggggc ccgacactta accgaagctc tttttctcct tatcaaaaaa
     5701 acatatccga tacatactat ctagctgttc aaaacatctt tcttgcagta gtttattttg
     5761 actatgaaat ctcccctaga acatccacaa gaactaatca ctatttcaaa ttgctcaatc
     5821 aacaaccagg aaacaacgat actttctcat ctatccctca tcatcaaagc cggtcacaat
     5881 tggttaatca ccggatcaaa tggtagtggc aaatctgccc ttggagcagc cctttgccaa
     5941 tccaaggatc tttccattat tccacatgat ggaggccttt attcaaacac actgtcttct
     6001 tttatatccc gcgtttcttt tgaactagcc gcctctctaa tagaggatga acataaaagg
     6061 gatgactcgg attttattga aggcggaatc gactcaggta gaacaccccg caaccttctt
     6121 gataatcctt ctaatcaaaa tccctatgtt atgttaacgg ggatcgaagc ctttcttgat
     6181 accggattaa aatttctttc tacgggtgaa attcgcag
//
LOCUS       DAOQXQ010000014         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_13559_length_5977_cov_2.921310, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000014 DAOQXQ010000000
VERSION     DAOQXQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13559_length_5977_cov_2.921310"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2061)
                     /locus_tag="PKH81_00490"
     CDS             complement(<1..2061)
                     /locus_tag="PKH81_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ96554.1"
                     /translation="MRKFWIKITQAFLSLAGCILLSCSVDYAPREDVVDFVKAGLNEI
                     RLSSYTVTIDPADANVEGLAHAVPGYVNQILISLSNPRLFTAEYALLTDVPDLFTPEG
                     QPTLEVVSPVSLRITGIPRVDLEDETALSFRITVRIPETNRSCPDITLSIPCRHISSA
                     VEVALDLSQGASQAMFFTPNPVQVGVGSSLVISLPSASPLNAGTDWVWYVNGVVQSGA
                     TSSSYTFNPSGWLRGQYTVSAFTTLNGVRYSGNLSVLVVDPLLFPLRYDLAGGSATGL
                     SLVSAYAPGAPVTVFSGTLTPPSGLSGFSGWETSDLGTPGQLLSGGATFAMPSNAVLL
                     RARWSGYPATSNLSALAGNASVRLDWTDPASSAFLFHVLSWQNSAGTTMGGIQVPAGL
                     GSYTVGGLSNGETYTFTLTGHFSGTAETVLASVRSTPREPSVVDQLVDVPCVTNFLQD
                     YSGSTFNNRLDPFRIGRTEVTYERWYAVRQWAHANGYVNIRPGKEGSSGVAGTVPQGS
                     LQPVTDIMAKDVVVWCNALSEMNGLELAYYSDAGGTQVRRNVDAPGSICYKPSANGFR
                     LPTLGEWQYAARYRDGSSWTPAGSPSVPSGTAVVSYPEYAITGTSGTANVASRLANPL
                     GLYDMSGNLAEMVWDRFVGAPPSATDIAYNLFGNQNTVKQALGGAYSGAITVSNPTYI
                     SSPSD"
     gene            2206..3474
                     /locus_tag="PKH81_00495"
     CDS             2206..3474
                     /locus_tag="PKH81_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96555.1"
                     /translation="MKSTTRLSFLFLSALLLGACATGPDLFIAKQIPVADKADMLFRN
                     GKIQYEEQLVKNDDLTAIPRVRKYFENTLEIDPIHPEAEAWIVKVDRFSKKSFDDNIA
                     KAKRLRDKGKRSSAEDFAMVLAVKKAGDIKTFDKDLLFLRKDTSSVRKEQIQTRLDKL
                     TALQTDALATTNTRTLARIGSRSLPIYEQLEAIDPGNGDAKKAMIAINTHITSLAQTD
                     IDAAKKYLEGRHYGDAEGAILRAEKALAGTAAASSEEITTIKYQIYYRWASALYLLNK
                     WNLSNDKVNRALAIKRTNEAVALKAKLNKGTAETPRDYDSEAADIIADIDAMIARGQL
                     SQALSRIETSVPLMKKKENQDAISSRKAAVMEAVKKTYNEGIASYNEEDYEMARDRFT
                     QVVRIQGDYEQAQAYLDRTNGKLKALSGSN"
     gene            3499..5778
                     /locus_tag="PKH81_00500"
     CDS             3499..5778
                     /locus_tag="PKH81_00500"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ96556.1"
                     /translation="MNKRAVISRIFDTIAAGAFIAWFFMPIAPGPVDRSTAGLLLKPF
                     TLPLVMQELGTAMFSPHIYWIVYFVPLVGLYRIVSLFFLKKLGRLAAPGGILLSLARI
                     IATIIAMAATIFPAILFADNPMWFLAIPREALISIGVIFLIHIVSLKALATLINNQNA
                     VYREYRQFKSEQNLNLPALRKDSGEKKRKAANALEVLFRIRTKLFFAFIGIISIILLV
                     LSTLLLKSYRVTILNAVGDAARGQVLQASANYRINLGDNIAMFEYLNRQRELNAHAEF
                     AYETLTLYTLLNQEIYLDALTKELPDFRAEYASRNPGEQFPTVEPLSGARAKELALAY
                     QAQQNVLVERNEAERTMSYISPIIKIDTVRKGDERVRRERLLGYSVMVFSEDVIFKPF
                     FKTRVLVIVITALFLYVAVILTYLVGNYIVNPLLFLRMNVRKISNVLTTMIRGTARVS
                     SANLVYNDCVTSRDELKSLSTEINDMVTVIRGIVPYISASTLKQAESGKASTARKELC
                     FLFTDIRGFTTLCEGMQPEEVVHVLNSYLDLETEIILNNHGDVDKFVGDEMMAFFEGP
                     LKEVNACRAAMQIRAAMMAEKERREKEGKPIVAIGIGINSGDVVFGSVGARDRMDFTS
                     IGDTVNLAARLEGANKAYGSKSIITEAVYERVQDNFLCRELDFIAVKGKTEPVRIYEI
                     LQEIPQAIPKLVEIKTLFEKGLKYYRSGSWDRAISAFTKNAELYEDAPSMVFIERIQH
                     FKKDPPPKGWDGVFRMTVK"
BASE COUNT         1631 a         1610 c         1484 g         1252 t
ORIGIN      
        1 atcagaagga ctagaaatat acgttgggtt cgagacagta atagctcctg agtacgcacc
       61 gccaagggcc tgcttaacgg tgttttgatt tccaaatagg ttatacgcta tatcagtagc
      121 cgaaggagga gcaccaacaa atctatccca caccatttcg gcgaggttgc cgctcatgtc
      181 ataaaggcca aggggattgg caagacggct cgcaacattt gccgttccgc tcgttccggt
      241 aatggcatat tcaggatagc tgacaacggc ggtaccggaa ggaacgctcg gagaccccgc
      301 cggggtccaa gaagagccgt cccggtagcg ggccgcgtat tgccactcgc caagcgtcgg
      361 caagcggaaa ccgttcgcgg atggtttata acaaatggaa ccaggggcat caacgtttcg
      421 gcgaacctga gtgccccccg cgtctgaata atacgcgagt tcaagaccgt tcatctcgct
      481 cagggcgtta caccagacca ccacatcctt tgccataata tcggttaccg gctggagact
      541 tccctgcgga acggtcccgg ctactccgga agagccttcc ttcccggggc gaatatttac
      601 atacccgttt gcatgggccc actgacgcac cgcataccag cgctcgtacg tcacctcggt
      661 tcgcccgatc ctgaaggggt caagccggtt attaaaagta gagcccgaat aatcctgcaa
      721 aaagttagtc acgcagggaa cgtccacgag ctgatccacc accgaaggtt cgcgcggggt
      781 ggagcgaacc gaagcaagaa ccgtctctgc cgtgcccgag aaatgaccgg taagggtaaa
      841 ggtataggtt tccccattgg aaagaccgcc caccgtgtac gagccaagcc ctgcgggaac
      901 ttgaatcccg cccatcgtcg ttcctgcgga gttttgccag gaaagcacat ggaaaagaaa
      961 ggcagatgag gcgggatcag tccagtcaag ccgaaccgaa gcgttccccg ccaaggcaga
     1021 aagattactc gttgccggat agcccgacca acgggcgcgc aagagcacgg cattggacgg
     1081 catcgcaaag gtagccccgc cggaaaggag ctgacccggg gtaccaaggt cggaggtttc
     1141 ccagccggaa aaaccggata aacccgaagg cggcgtcagg gtgcctgaaa aaaccgtaac
     1201 cggagccccc ggggcatagg cacttaccaa agaaagcccg gttgcactgc cgcctgcaag
     1261 atcatagcga aggggaaaca agagaggatc aacgaccaac acagacagat tgcctgaata
     1321 gcgaaccccg ttaagggtag taaaggcgga cacggtatac tgaccgcgca gccaaccgga
     1381 tggattaaaa gtataactgg aagaagttgc gccactttgg acgaccccgt ttacatacca
     1441 gacccagtcg gttcctgcgt taagggggga tgcagaggga agggaaataa ccaaagaaga
     1501 tccaaccccc acctgaacag gattaggggt aaagaacatc gcctgacttg caccctgcga
     1561 aaggtccaaa gccacctcaa ccgccgagga aatatgacgg caagggatag aaagggtaat
     1621 atcgggacag ctccggtttg tttcagggat cctgaccgtt atcctgaagg acagggccgt
     1681 ttcatcttcc aggtccacgc ggggaatacc ggtaatgcga agggacacgg gggatactac
     1741 ctcaagggtt ggctgaccct ccggagtaaa aagatcaggt acatcggtca acagggcata
     1801 ttcagcggta aaaaggcggg gattagaaag agaaataagg atttgattta cataaccggg
     1861 caccgcatga gcaagaccct ctacgttggc gtcggcagga tcgattgtta ccgtatatga
     1921 agaaagacga atctcgttaa gacctgcctt aacaaagtcc accacatctt cccgaggggc
     1981 ataatcgacc gaacaggata aaaggataca gcctgccaga gagagaaacg cttgagtgat
     2041 ttttatccaa aacttgcgca tttccggggg cccttaactt taaaaaagaa tgaaactaat
     2101 ataccattca gtatacaatg ttacaagaaa gcatccagat tattgatacc ttttagtgga
     2161 tgaagtaaac taaccctgct ataaagaaag ctaccggagg ataccatgaa aagtactaca
     2221 cgtctatcat tcctgttcct tagcgcgctc ctgcttggcg cctgcgctac cgggccagat
     2281 ctgtttatcg caaaacaaat acccgttgcc gacaaggccg atatgctctt tcgcaacgga
     2341 aagatccaat atgaagagca actcgttaaa aacgacgacc tgaccgctat cccccgggtt
     2401 cgcaaatact ttgaaaacac cctggaaatt gaccccattc atccggaagc agaggcctgg
     2461 attgtaaaag tagaccgctt tagcaaaaaa agctttgacg ataacatcgc caaggccaaa
     2521 agactccggg acaagggaaa aagaagctcg gcagaggact ttgccatggt tctggcggtt
     2581 aaaaaagccg gagacataaa aaccttcgac aaggacctgc tctttttacg aaaagatacc
     2641 agcagtgtgc gaaaagaaca gatccaaacc cgattggaca agcttaccgc actacaaacc
     2701 gacgcccttg ccaccaccaa tacccgaacc cttgcccgta tcggcagcag aagtcttccc
     2761 atatacgaac agctcgaggc gattgatccg ggcaacgggg acgcaaaaaa ggctatgatc
     2821 gccatcaaca cccatatcac ctccctggct caaaccgata ttgacgctgc aaagaaatac
     2881 ctggaaggca gacactatgg ggatgcagaa ggcgcaatcc tccgcgcgga aaaggccctt
     2941 gccggaaccg ctgcggcttc ctccgaagaa attaccacga ttaagtacca aatctactac
     3001 cgttgggcct cggccttgta cctgcttaac aagtggaacc tctccaacga caaggtaaac
     3061 cgcgcgcttg cgattaagcg aacaaacgag gccgtcgcgc tcaaggccaa gctgaataag
     3121 ggaacagccg agaccccccg ggactacgac agcgaagcag ccgacattat cgccgacata
     3181 gacgccatga tcgcgcgcgg acaactgtcc caggccctgt cccgcatcga aacaagtgtt
     3241 ccgctcatga aaaagaaaga aaatcaggat gccatctcat cgcgtaaagc agctgtcatg
     3301 gaagcggtaa aaaagaccta taatgaggga atcgcatctt acaacgaaga ggactacgag
     3361 atggcgcgcg accgctttac tcaggttgtt agaattcaag gcgactacga acaagcccag
     3421 gcctatctgg accgcaccaa cggtaagctc aaggcgcttt cgggatctaa ttaaccgtcg
     3481 tttacaagga agtccggtat gaataaaaga gctgtaatct cacgtatttt tgacaccatc
     3541 gcggcaggcg cctttatcgc ctggtttttc atgccaatag ccccaggacc ggtggacaga
     3601 tcaaccgccg gtctccttct taaacccttt accctgcccc tagtaatgca ggagctggga
     3661 acggcgatgt tctcgccgca tatatactgg attgtctatt ttgtccccct tgtcggtctg
     3721 taccgcattg tctccctgtt ctttttaaag aagcttggcc ggcttgccgc tcccggagga
     3781 atccttttat cccttgcccg catcatagca acgattatcg ccatggcggc cactatcttt
     3841 ccggcaattc tttttgcgga taacccgatg tggtttttgg ccattccccg cgaagcgctc
     3901 atctcgatcg gggttatctt tttaattcac attgtttcgc tcaaggccct ggccaccctg
     3961 attaacaatc aaaacgccgt ataccgagag tatcgccaat tcaagagtga gcaaaatctg
     4021 aatttgcccg cactcagaaa agactctggc gagaaaaaac gcaaggcggc aaatgcgctc
     4081 gaggttctgt tcagaatccg gaccaagctc ttttttgcct ttatcggcat catctcgatt
     4141 atcctgctcg tcctgagtac cctgctcctt aaaagctacc gcgtaaccat actgaacgcg
     4201 gtaggagacg ccgctcgcgg ccaggtcctg caggcaagtg cgaactatcg cattaatctg
     4261 ggcgacaaca tcgccatgtt tgaatatctg aatcgacagc gcgagcttaa cgctcatgcc
     4321 gagttcgcct acgaaaccct aaccctgtac accctgttga atcaggaaat ttatcttgat
     4381 gcgcttacca aggaactacc cgacttccgc gcagaatacg cctcacgcaa ccccggcgag
     4441 cagttcccca ccgtagagcc gctttcggga gccagggcaa aagaacttgc actcgcctac
     4501 caagcccaac aaaacgtctt ggtggaacga aacgaggccg agcgcaccat gagctacatt
     4561 agccccatta tcaagataga caccgtgcga aagggcgatg agcgcgttag aagggagcga
     4621 cttcttggat actctgttat ggtattcagc gaggatgtaa tctttaagcc cttctttaag
     4681 acccgcgttt tggtcatcgt aattactgcc ttgttccttt atgtcgcggt tattcttacc
     4741 tacctggttg gaaactatat cgttaacccg ctcttgttcc tcaggatgaa cgtacgaaag
     4801 atatccaacg ttcttaccac aatgattcgc ggtaccgccc gcgtctcttc cgcaaacctg
     4861 gtgtataacg attgcgtaac cagccgggac gagcttaaat ccctttccac cgagattaac
     4921 gacatggtta ccgtcatccg cggtattgtg ccctacatct ccgcctccac ccttaaacag
     4981 gccgagtccg gaaaggcctc cactgcccgt aaggagcttt gcttcctctt taccgacatc
     5041 cgcggtttta ccaccctctg cgaaggcatg cagccggaag aggtggttca tgttttgaat
     5101 agctacctgg acctggaaac cgagattatt cttaataacc acggggacgt agacaagttt
     5161 gtcggcgacg agatgatggc cttttttgaa ggtccgctta aggaagtaaa cgcctgccgc
     5221 gcggccatgc aaattcgcgc tgccatgatg gcagaaaaag aacgtcgcga aaaagaaggc
     5281 aagcctattg tggctattgg tattggtatc aattcagggg atgttgtttt tggctccgtt
     5341 ggcgcgcgcg atcgtatgga ctttacctcc atcggcgaca cggtaaacct tgccgcccgt
     5401 cttgaaggcg cgaacaaggc ctatggctct aaatcaatta tcaccgaggc agtatacgaa
     5461 cgggttcagg ataacttcct gtgccgtgag ctcgacttta tcgcagtaaa agggaaaaca
     5521 gagcccgtgc gcatctacga aatactgcaa gaaattcccc aggcaatacc caaactggtg
     5581 gagattaaaa ccctcttcga gaaaggcctt aagtactacc gatcgggaag ctgggaccgg
     5641 gcaatctccg cgtttaccaa aaacgccgag ctctacgagg atgcaccttc tatggtgttt
     5701 atcgagcgta tccagcactt taaaaaagat ccgccgccca agggttggga tggcgttttc
     5761 agaatgaccg tcaagtaaca aaaaaggaaa gggtttcccc ctttcctttt ttttatctct
     5821 atcgctcttc tctgaaataa gacaggccaa gagactcagg cccctgatgc tcctttttac
     5881 ggcgcatggt aatcagggct aatacaacga tggttgcaat ataaggaatc atatccagta
     5941 tctggggcga aaaggcgatg tgcttaccaa agacctc
//
LOCUS       DAOQXQ010000015         5880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_13858_length_5880_cov_2.943863, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000015 DAOQXQ010000000
VERSION     DAOQXQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13858_length_5880_cov_2.943863"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PKH81_00505"
     CDS             <1..1493
                     /locus_tag="PKH81_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ96557.1"
                     /translation="TLKALFYACFPLSIVFVFRFFRQFFGIRTGRKTMMAISAVGFLF
                     ACGYFFMGTTVALDSWHSLMLLYPIAAIVYGFYGSLRCLLKGRLETLSTILGLVVAVA
                     FSAYDIYFFLGNHTPMILLQGIGFMSMILGNFYSFSQEIADTNRKCALYADEMERNKV
                     QRDRLFQKIREDTIKSEESTHTLGLSIQQVGTLMTQYLASIDNINSSIETQSDQVSQN
                     KDHVERIFSSIQETSQMVEQHEKLVEITVSTVRELTEGIHRTDTLVKASGETLQKLTS
                     VCLAADRDVAESLKFVEDLADYSENITEIVKSISDLAEQTNILSINAAIEAARSGQMG
                     KGFAVVASEIRSLATRSGESADQIRTILGTMLDKIRVIQRQETLVSSRLKDIVGENEL
                     IDRSITEIFTVLENQLSRNERIGQTIQELVQAVHRIAEQATDQKNSGENLKDSLVLLE
                     AITSSILTASREQRSCNEELKDNLEQLTDVSRKNQDVVHDLKELIV"
     gene            1542..1844
                     /locus_tag="PKH81_00510"
     CDS             1542..1844
                     /locus_tag="PKH81_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNQ96558.1"
                     /translation="MLKHIVMWQFKDEAEGKTRSENCRHIRGLLESLVGVVPEILSLE
                     VGINEYPSPQSSDMVLTVEFESKEALDRYTAHPEHVKVSTYVGKVRTSRSVVDYLI"
     gene            1841..2476
                     /locus_tag="PKH81_00515"
     CDS             1841..2476
                     /locus_tag="PKH81_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HNQ96559.1"
                     /translation="MSSSEVERHVNWFALEKACGRAGCPVCSIVEERTTRYIDNMLFE
                     HVSDRGFRAQYRLAQGFCTRHGKNLESFRDGLAVAILGEDILQSLMAEIQSRKFKKTK
                     ALCPACSAEQTVEREFLGYIAERTEHEFIEFFSRSSLLCVPHYRALCVTTKKIPRWLI
                     ESHEARYASLLQRVRSFIECSAWGRQTDFDALSDEDKIVWKELARTLRGSR"
     gene            2531..3340
                     /locus_tag="PKH81_00520"
     CDS             2531..3340
                     /locus_tag="PKH81_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ96560.1"
                     /translation="MKRITFALIALTLCVSAITAGGKKDVGADNSLATIMERKTFVMG
                     LDDSFPPMGFRNENNEIVGYDVDLAREVTRRMGIELKLQPIDWNAKEQELNTGKIDCI
                     WNGFTITPERAKVITFTKPYLKNAQVLVVRKDSKYLSLADLSGKTVGLQAGSSAAGAL
                     ENATEFRSTLKGVVEFKDNMLALMDLEAKGVDAVVMDLMVANDNINRSGKSYRILSED
                     LAPEEYGIGFRKSDVQLMQAVQKTLEEMAGDGTVSKIAVQWFGADISTIGK"
     gene            3425..4060
                     /locus_tag="PKH81_00525"
     CDS             3425..4060
                     /locus_tag="PKH81_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNQ96561.1"
                     /translation="MLKILQAMLEGTVVSLEVFALTLLFSLPLALPVALGRISKQPLI
                     RALVNGYLLVMRGTPLILQLIFVYFAPYYLFSLSYDRFTAVIVAFTLNYTAYFAEIYR
                     GGIESIPRGQYEAAKVLGLTRFQTFFRIVLPQVIKRILPSMGNEVITLVKDTALAQTI
                     GVAELFRVAQNASSRQFSTMPIFLAGVFYFVMNWAVSAVFVRFEKKLSYYH"
     gene            4077..4832
                     /locus_tag="PKH81_00530"
     CDS             4077..4832
                     /locus_tag="PKH81_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNQ96562.1"
                     /translation="MEIIKVENLSKSFGSLKVIRDISFTVNSGEVLAVIGPSGSGKST
                     LLRSITHLETVSGGTVTIGGEDMVRDGQYASPSQLRSICLKVGLVFQNFNLFPHLSVM
                     DNVAEAQIRVLNRTRNHAEARARELLGKIGLADKEHSYPCELSGGQQQRVSIARALAL
                     NPQVLFFDEPTSALDPELTGEILKVIRTLAAEKMTMVIVTHEMHFARDVADRVIFMDE
                     GVIVEQGPARSVIEQPRSERTKAFLSRFSSRDA"
     gene            complement(4859..>5880)
                     /locus_tag="PKH81_00535"
     CDS             complement(4859..>5880)
                     /locus_tag="PKH81_00535"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNQ96563.1"
                     /translation="RLNEVHDKADILNRSFETPLYVQGSLIHGSVSIGIALYPMHSQD
                     HGELLKCADIAMYRSKKEGKNRAILFNISMMAPIHERMKIGEHLHSALARMEFELYMQ
                     PQILVETGKIVSLEALLRWNSPVLGRVPPDKFIPVAEETHLIVQIGDWVLREACRFLK
                     KMHKEGYTRLSVSVNVSMVQLLQQDFPSSVAKILSEYGIDSSFIELEITESVLMGSGE
                     AIAKGLQALNEIGVRIALDDFGKGYSSLAYLEQLPITVLKIDKSFIDGITPESTAKSI
                     TGNIVGIGKKLGLEVIAEGVENENQLTYLAEQQCDKIQGWIYSKALPLEEAEQFIRGN
                     LGSPM"
BASE COUNT         1477 a         1301 c         1418 g         1684 t
ORIGIN      
        1 ttacccttaa agctcttttt tatgcgtgtt ttccgctgtc catcgtgttc gtgtttcgat
       61 ttttcagaca gttttttggc attcgcaccg gtagaaagac gatgatggct atttctgcgg
      121 ttggtttcct cttcgcttgt ggctatttct ttatgggaac gactgttgcg cttgattcct
      181 ggcattccct tatgttgctg taccccatag ctgctattgt ctatggtttt tatgggtccc
      241 ttcggtgtct gcttaaggga agactcgaga ccctttccac tattttggga cttgtcgtcg
      301 ccgttgcctt ttccgcctat gatatctatt tctttttagg caatcacaca cccatgattt
      361 tgttgcaggg tattggtttt atgagcatga ttttgggaaa cttttatagt ttctcacaag
      421 aaatcgccga tacaaaccgt aaatgtgccc tgtatgccga cgaaatggaa agaaacaagg
      481 ttcaacggga tcgattgttc caaaagattc gggaagatac gataaagagt gaagaatcaa
      541 cccacaccct tggtctgagt atccaacaag ttggcacgct gatgacccag tatttggcaa
      601 gtatagacaa cataaactcc agtatcgaaa cccaaagcga tcaagtaagc caaaacaagg
      661 atcatgttga gcgcattttc tcttcgattc aggaaacctc ccagatggta gaacaacatg
      721 aaaaactcgt ggagattacc gtttcgacgg tcagggagct taccgagggt attcatcgca
      781 ccgacaccct ggtaaaggcg tctggagaga ccttacaaaa actaacctcg gtgtgtcttg
      841 ctgccgaccg ggatgttgcc gaaagcctta aattcgttga ggaccttgct gactattcag
      901 agaatattac cgaaattgta aaatccatca gtgatctggc agagcaaacg aatattctgt
      961 ccataaatgc ggcaattgaa gctgctcgtt caggccagat gggaaaaggt tttgccgtcg
     1021 ttgcttccga gatccgctcg cttgccactc gctccggcga aagcgccgat cagattcgca
     1081 ccatcctggg aacgatgctc gacaagattc gggttattca gcggcaagaa acccttgttt
     1141 cgtcccggct taaggatata gtcggcgaga acgaattgat agatcgatct attacagaga
     1201 tatttaccgt gttggagaat caactttccc gcaacgaacg aatcggtcag accattcagg
     1261 agcttgtgca agcggttcac cgtattgccg aacaggcaac agaccaaaag aacagcggag
     1321 agaatcttaa ggattccctg gttcttcttg aagcgataac ctcgtctatc ctgactgctt
     1381 cccgtgaaca gagatcctgt aatgaagagc ttaaggataa tcttgagcag cttaccgatg
     1441 tgtctcgcaa aaatcaggat gtagtacacg atctaaaaga actgattgtg taggctgtcc
     1501 cagatacccc gggatatgat acactgttcc cggaggaaca tatgcttaaa cacatcgtta
     1561 tgtggcagtt taaggacgaa gccgaaggaa aaacgcgcag tgaaaactgt cgacatatac
     1621 ggggacttct ggaatccctg gtgggggttg tccctgaaat tcttagtttg gaagttggga
     1681 ttaatgaata tccctctcct caatcctcgg atatggtttt gaccgttgag tttgaatcga
     1741 aggaagccct tgatcgctat accgcgcatc ctgaacacgt caaggtaagc acctatgtgg
     1801 gcaaggtcag gaccagtcgt tctgttgtgg attatctgat ttgagttcaa gcgaagtaga
     1861 gcggcatgta aactggtttg cccttgaaaa agcgtgtgga agggcgggct gtccggtttg
     1921 ttcaatagta gaagagcgca ccacccgata tatcgacaat atgctttttg agcatgtctc
     1981 tgatcgtgga ttcagggcac agtatcggct ggcccagggt ttttgcaccc gacatggaaa
     2041 gaatttggaa tcctttcggg atggtttggc cgtggcaatt ttgggagaag acattcttca
     2101 atctttgatg gcagagatac aaagcaggaa attcaagaaa acaaaggcgc tctgtcctgc
     2161 ctgttctgct gagcaaacgg tagaaaggga atttcttgga tatattgccg agcgaacgga
     2221 gcatgagttt atcgagtttt tttctcgatc ttccctgcta tgtgttcctc attatcgcgc
     2281 tctttgtgtc accacaaaaa aaattcctcg ctggttaatc gaaagccacg aggctcgata
     2341 tgcctcgtta ttgcagcggg ttcgctcatt tatagaatgt tctgcttggg gacgtcaaac
     2401 tgattttgat gccttgagcg atgaagacaa gatcgtatgg aaggagcttg cgcgtacgtt
     2461 gcggggatcc cggtaacgga acgattaagc atacataaaa agctagtacg caattacaag
     2521 gagaactaac atgaaaagaa tcacatttgc tctgatcgcg cttaccctct gtgtttccgc
     2581 gattaccgcc ggcggaaaaa aggatgtggg agcggacaat tctcttgcta ccataatgga
     2641 acgcaagacc tttgtgatgg gacttgatga ctcgtttccc cctatgggtt ttcggaacga
     2701 gaacaacgag atcgttggat atgatgttga tcttgcgcgc gaagtaaccc gccgcatggg
     2761 cattgagctc aagcttcagc cgatagactg gaatgccaaa gagcaagagc ttaacactgg
     2821 caagattgac tgtatctgga atggctttac cattacaccc gaacgtgcaa aggtaattac
     2881 ctttacaaaa ccatatctta agaacgccca agtgttggtc gtacgaaagg attccaagta
     2941 cctttctctc gctgatcttt ctggaaagac ggtgggcttg caggctggct cttccgctgc
     3001 aggtgctctg gaaaatgcga ccgaattccg ctctaccctt aagggagtag tggagtttaa
     3061 ggacaatatg cttgccctta tggaccttga ggcaaaaggc gtagacgcgg tagtgatgga
     3121 cctgatggtt gctaacgata acattaaccg ctccggaaaa agctatcgta tcctttccga
     3181 ggatcttgct ccagaagagt atggcattgg ttttagaaag tctgatgtac agctgatgca
     3241 agcagtacaa aaaacccttg aggaaatggc tggcgacggt acggtctcaa agatcgctgt
     3301 acaatggttt ggcgctgata tttctaccat cggtaaataa ggcccatttt tattacgttc
     3361 acaagggaag gggatatccc cttcctttct tttttctgta tacttgctaa tcaggaagta
     3421 ctgtatgctt aagattttac aagcaatgct tgagggtacg gttgtatccc ttgaggtgtt
     3481 tgccttaacc ctgctatttt ctcttccgct tgccttaccg gtcgcccttg ggcgtatatc
     3541 caaacaaccg cttattcgcg ctttggtaaa cggatacttg ttggttatgc ggggcacacc
     3601 gctgatcctt cagcttattt ttgtgtattt tgccccttat tatctttttt cgctttccta
     3661 cgatcgcttt accgcggtta ttgttgcgtt taccttaaac tacactgcat attttgcgga
     3721 gatttatcga ggcggcatcg aatccattcc ccgcggtcaa tatgaggcgg caaaggtgct
     3781 tgggttaacc cggtttcaaa cttttttccg gattgtcctt cctcaagtta tcaagagaat
     3841 tctaccgtct atgggtaatg aagtaattac cctggttaaa gataccgctc tggcgcaaac
     3901 gatcggagtt gccgaattat ttcgcgtagc tcaaaatgcc tcgtccaggc agttttctac
     3961 catgccgatc tttctggcgg gtgtcttcta ttttgtaatg aattgggcgg tatctgccgt
     4021 ctttgttcgt tttgagaaaa aactttcgta ttatcattaa aggacgaact gccaatatgg
     4081 aaataatcaa ggttgagaat ctttctaaaa gctttggctc cctgaaagta attcgcgaca
     4141 tttcgtttac ggttaattct ggagaggtgc ttgcggtcat cggtccctcc ggctcgggca
     4201 agtcaaccct gcttagatca attactcatt tagagacagt ttctggtggt acggttacga
     4261 ttggtggaga ggatatggtt cgggatgggc aatacgcaag tccttcacag cttcgttcga
     4321 tctgtcttaa agtgggtttg gtttttcaga actttaattt gtttcctcat ttatcggtga
     4381 tggacaatgt tgccgaggcg caaatcaggg ttcttaatcg aacaaggaat catgccgaag
     4441 ctcgtgcgcg tgaattgctg ggaaaaatag ggcttgcgga caaggaacac tcgtaccctt
     4501 gtgaattatc gggtggccaa cagcagcgag tatccatcgc tcgagccctt gcccttaatc
     4561 ctcaggtgct cttttttgat gaaccgacga gcgcccttga tccagaatta actggcgaga
     4621 ttcttaaggt aatacgtacc cttgctgccg agaagatgac gatggttatt gtaactcatg
     4681 agatgcattt tgcgcgtgac gtagccgatc gcgtcatttt tatggatgaa ggggttatcg
     4741 tggagcaggg gccagcccga agcgttattg aacagccccg ttctgagcgg acaaaggcct
     4801 ttctttcccg tttttcatcc cgggatgcct gatcattcgc aaaaaaaagt ggtgctattt
     4861 acattggcga accaaggttt ccgcgaataa attgctctgc ttcctctagc ggtagggcct
     4921 tgctatagat ccatccttgt atcttgtcgc attgctgttc ggctagatac gtaagctggt
     4981 tttcgttttc aacaccttct gctattacct caagacccag tttcttacca ataccaacaa
     5041 tgtttcctgt tattgattta gcggttgatt caggggtaat gccgtctata aagctcttgt
     5101 cgattttcag gacggtgatg ggtaattgct caaggtaggc aagcgaggaa tatcctttac
     5161 caaagtcatc caatgcgatg cgaacgccta tttcgttaag ggcttgtaag cctttggcta
     5221 ttgcttcccc cgagcccatc aggacagatt cggttatttc gagttcaata aatgaactgt
     5281 caataccgta ttcactgagt atcttggcga cggaagaggg gaaatcttgc tgaagtagtt
     5341 gaaccatgga aacgttaacc gaaacactca gtcgtgtgta tccctctttg tgcatcttct
     5401 taaggaagcg acaggcctct cttagcaccc aatccccaat ttggacgatc agatgggttt
     5461 cttctgctac cgggataaac ttatccgggg ggactctgcc aagaaccgga ctgttccatc
     5521 gaagcagggc ttccaaactg acgattttac cagtctctac cagaatttgc ggttgcatat
     5581 aaagctcaaa ctccattcgg gccaaagcag agtgtagatg ctccccaatc ttcatccgtt
     5641 catggatggg tgccatcatg gaaatattaa aaagaatcgc tctgttttta ccttcttttt
     5701 tggagcggta catcgcaata tctgcgcatt ttagcagttc cccatgatct tggctgtgca
     5761 tggggtataa ggctatacca atactaaccg agccatgtat gagtgaaccc tggacataca
     5821 agggagtttc aaaggatcta tttaaaatat ctgccttgtc atgcacttcg ttgagtcgat
//
LOCUS       DAOQXQ010000016         5863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_13901_length_5863_cov_3.072831, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000016 DAOQXQ010000000
VERSION     DAOQXQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_5863_cov_3.072831"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1691)
                     /gene="malQ"
                     /locus_tag="PKH81_00540"
     CDS             complement(147..1691)
                     /gene="malQ"
                     /locus_tag="PKH81_00540"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNQ96564.1"
                     /translation="MKAPRSSGILLHPTSLPGTPGIGTIGSPAYRFIDWLSLAGQSLW
                     QVLPLGPTGYGDSPYASFSTFAGNPLLIDLQMLQDQGLLSAQDTQLPEYIRTTGPIDF
                     GSVVWWKIPLLKKAAAHFLSTQAGSDYNQFIKEQAYWLDDYALFMNIKAFYDKKAQDE
                     GLSGKLWHNWWPRALATRDKKALLQWKEENKAGIEEEKAIQYLFYGQWKALKQYAHSK
                     GISIIGDIPIFVAADSADVWANQKYFQLTDTGSPKAVAGVPPDYFSETGQLWGNPLYN
                     WKALKKDGYSWWLKRITSTLSMVDYVRVDHFRGFEAYWSVPAEEATAINGQWIQGPDH
                     DFFKTIRASLGELPILAEDLGVITDGVRALRDDFGFPGMKVLQFAFDANEAGQNGFTN
                     AFLPHMYTANSIVYTGTHDNDTMKGWLDKADIREIDMIKDYLGVEGTDLVRDLIRAAL
                     MSVAVFAIFPLQDIYGIGSEGRMNEPSTLGSNWTWRMDESMYNPDQASWLKRLSRLYA
                     RNLEQA"
     gene            complement(1849..2532)
                     /locus_tag="PKH81_00545"
     CDS             complement(1849..2532)
                     /locus_tag="PKH81_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96565.1"
                     /translation="MVKQDLIERSPVRNFEKSLGGGVKAGEIGVIVSKKGLGKTSILV
                     QLGIDKLLQGSSVVHVSFNQQASYVMTWYEDIFGELAKKKNLDKAAEVQEDLISKRIV
                     LNFNQDSVKTAQVIKTIKALAEGGSKPEAIIIDGFNFSKANPDSMKEMKAFAKESGTV
                     IWYSANAESTDKGFPAALTPFKTDLDVVLYLDPKSDCIQIQVLKEHDKGDYSTELKLD
                     VKTLLIAEK"
     gene            complement(2543..4630)
                     /gene="fusA"
                     /locus_tag="PKH81_00550"
     CDS             complement(2543..4630)
                     /gene="fusA"
                     /locus_tag="PKH81_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ96566.1"
                     /translation="MAFDISKMRNIGISAHIDSGKTTLSERILFYCNKIHHIHEVRGK
                     DGVGAVMDNMELERERGITIASASTQVQWKDYTINVIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVGGVQSQSITVDRQLKRYHVPRIAFVNKCDRTGANPLKVRMQLREKLGLNA
                     YMAQLPIGLEDKLEGIVDLVTMKAVYFEGESGTEIRIAEIPAHLQADAQKYREELVDA
                     ASMFSDELAEAFLEGTETEEMIHAAIRKGTLAEQLVPVFLGSAYKNKGIQSLLDGVTR
                     YLPDPTEVKNYALDLDKNEEQVLLSCDEKSPIVALGFKLEDGKYGQLTYVRVYQGSLK
                     KGDELYNTRARKKFKVGRLVRMNSSTMEDINEGVPGDIVALFGIECASGDTFCGGGLN
                     YAMSSMFVPNPVIDLSITPKDKKSSDQMAKALNRFIKEDPTFHCFVDPESNQTIIQGM
                     GELHLEVYVERMKREYNCEVETGKPQVAYREAITQRADFNYTHKKQTGGSGQYGRVAG
                     FMEPIEEKDYEFVDSIKGGSIPNEYIPSCDKGFRESMKKGALIGFPIVGVRCTINDGQ
                     FHPVDSSDIAFQLASIGAFREAYLKAKPAILEPIMKVSIEGPQEFQGNIFGLINQRRG
                     IILSSTEDDNFTRVDAEVPLSEMFGFSTILRSSTQGKAEYSMEFAKYGKVPQSVGETL
                     MKEYEAKRKAEQK"
     gene            complement(4747..>5863)
                     /locus_tag="PKH81_00555"
     CDS             complement(4747..>5863)
                     /locus_tag="PKH81_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96567.1"
                     /translation="TALPSQVKNPVRKQTSARYRPITPQDRPLVQPGTGTLTEQTGLQ
                     IHTPQTRIALVANPLQPNAHPRWFAASHVLQEPLPPQVTLSFSLFSGSSPMDKTKSDS
                     WFLPIQSYPAVPIDLHAAQLAISLPFFTMSATGYTNTAPLTPDQRAIHSDVRTGWKWF
                     TIAGSWYRQDPEFLDFSLNHDPIEKQWIIAPAVNLPCPGAGRTRLEFQALYGEDTVST
                     DTWNQEWQKNAYTSVQGKIANRDFSFHIGARKEGESVDLSIVYKNYHCIYPNLSLTLG
                     ASGTTTDSTIEPDRCTNTKITVSSVFKAHRQVRLEASVVRTQENQESPPDSRFTVSTS
                     LTNREQKKRYLALHVKWNYSSLSNEQLLSFYFKMRLK"
BASE COUNT         1403 a         1314 c         1498 g         1648 t
ORIGIN      
        1 tcctacacct ttgaagagca ggatattctg ctggtagcgg gttcagagga acgtatcttt
       61 gccttttcac aaaattccga gcctgcccgt gctgcccttg gtggtctatt caggaacctt
      121 tttcccaaaa agaattgatg tgagtatcac gcctgttcca ggtttcgtgc gtataagcgg
      181 gacagtctct tgagccagga agcctgatcg gggttgtaca tagattcgtc cattcgccag
      241 gtccagttcg agccaagcgt ggacggctcg ttcatccgcc cctcacttcc gatgccatat
      301 atatcctgaa ggggaaaaat cgcgaatacc gcgacagaca ttaatgccgc cctgatcaga
      361 tctcgaacca ggtcagttcc ttcaacccct agatagtctt tgatcatatc tatctcgcgt
      421 atatctgcct tgtccaacca tcctttcatg gtatcgttat cgtgagtgcc ggtatagaca
      481 attgaattgg cagtatacat atgggggaga aaggcgttgg taaagccatt ttggcctgct
      541 tcgttggcat caaaggcaaa ctgcaaaacc ttcatgccgg ggaagccgaa atcgtcccga
      601 agagcacgta ctccgtcggt aataacgcca agatcctcgg caagaattgg tagctctcca
      661 agagaggccc tgatagtctt aaaaaagtca tggtcgggcc cttgtatcca ttgaccattg
      721 attgcagtgg cttcttctgc gggtaccgac caataggctt caaagccgcg aaagtgatct
      781 acccgaacat agtctaccat ggacaaggtt gaggtaattc gtttaagcca ccaggaatat
      841 ccatcttttt taagggcttt ccaattgtat aagggattgc cccataattg tcctgtttcg
      901 ctaaaatagt cgggaggaac accggcaact gctttggggc ttccggtatc ggttagctgg
      961 aaatacttct ggtttgccca gacatcggct gagtctgctg ctacaaatat gggtatatct
     1021 ccgataatcg agattccctt gctgtgggca tactgtttta gggccttcca ttggccataa
     1081 aagaggtatt gtattgcttt ttcttcttct attccggcct tgttttcctc tttccattgt
     1141 aaaagggctt ttttatcgcg agtggctaat gctcgtggcc accagttatg ccataatttg
     1201 cccgagagac cctcgtcttg tgccttttta tcgtaaaatg ccttgatatt cataaaaaga
     1261 gcatagtcat caagccaata tgcctgttct ttaatgaatt ggttgtaatc gcttcccgcc
     1321 tgtgtagaaa ggaagtgtgc tgcggctttt ttaagtaagg ggattttcca ccatactacc
     1381 gatccaaagt cgataggccc tgttgtgcgt atgtattcag gaagttgtgt gtcctgcgct
     1441 gaaagaaggc cttggtcctg cagcatctgt agatcaataa gcaagggatt ccccgcaaag
     1501 gtagaaaaag aggcataagg ggagtctcca tagccggtgg gtccgagcgg aagaacttgc
     1561 cacaggcttt gaccggccag agaaagccag tctataaaac ggtaagctgg tgaaccaata
     1621 gtaccaatac ccggtgttcc cggtaaggat gtgggatgta ataaaatgcc gctcgatcgt
     1681 ggtgccttca tggttactcc tttattgatg ctgctaaacc ggtttactcg attactatac
     1741 ggtatcttta tcgctcacgc aaggggattg cggtatataa agatgagtca taggcatagt
     1801 caaaaaaaaa ccgcctatac aggcggtttt ctttgttacg attggtggtt atttttccgc
     1861 aatcaaaagg gtttttacgt ccagtttgag ttcggtggaa taatcaccct tgtcgtgctc
     1921 tttaagaacc tgaatctgta tgcaatcact cttggggtca agatagagaa ctacatcgag
     1981 atcggtctta aagggtgtaa gtgcggcagg aaagcccttg tctgttgatt cagcgtttgc
     2041 gctgtaccaa ataacagttc cgctttcctt ggcaaaggcc ttcatttctt tcatgctgtc
     2101 gggattcgcc ttggaaaagt taaatccgtc aatgatgatt gcttcgggtt tggatccgcc
     2161 ttctgcaagc gctttgatgg ttttaataac ctgggcggtt tttaccgagt cttgattaaa
     2221 attaagaacg atacgcttac tgatcaggtc ttcctgaact tcggcagctt tatccaaatt
     2281 ctttttcttg gcaagctctc caaaaatatc ctcataccag gtcattacat aagaagcctg
     2341 ctggttaaag gagacatgga cgactgaact gccctggagc agtttgtcta tgccgagctg
     2401 aacaaggatc gaggtttttc ccagaccctt tttggataca attaccccaa tctcgccagc
     2461 tttgacgccc ccgccaaggg acttttcaaa gttacggaca gggcttcgct caataagatc
     2521 ctgctttacc attgctatct ccttatttct gttctgcctt gcgcttggct tcgtattctt
     2581 tcatcagcgt ttctccaacg ctctggggga ccttgccgta ttttgcaaac tccattgaat
     2641 actctgcctt tccctgggtc gaggagcgga ggatggtcga gaaaccgaac atctcgctga
     2701 gcggaacttc cgcgtcgacg cgggtaaagt tgtcgtcttc ggtggacgac aggattatac
     2761 cacggcgctg attgataagt ccaaagatat ttccctggaa ttcctgaggt ccttcgatgg
     2821 aaaccttcat gatcggttca aggatagcag gttttgcctt aagatacgct tcacggaacg
     2881 cgccaataga ggcgagctgg aaggcgatat ccgaagagtc aaccgggtgg aactgaccgt
     2941 cgttgatggt gcagcgaact ccgacgattg ggaagccaat aagagcgccc tttttcatgg
     3001 attcgcggaa acccttatcg cacgagggaa tgtactcgtt agggattgat cctcccttga
     3061 tggagtcaac aaactcgtag tctttttcct caataggctc cataaaaccg gcaactcgac
     3121 cgtattgtcc ggaaccaccg gtttgcttct tgtgggtata gttaaagtct gctcgttggg
     3181 taatcgcttc gcgataggct acctggggct taccggtttc tacctcgcag ttatattcgc
     3241 gcttcatgcg ctctacatat acttcaaggt gaagctctcc cattccctgg ataatagtct
     3301 gatttgattc aggatcaaca aaacagtgga aggtggggtc ttctttgatg aagcggttaa
     3361 gggccttagc catttgatcc gaggattttt tatccttggg ggtgatggac aggtcgataa
     3421 ctggattcgg aacgaacatt gagctcatgg catagttaag tccgccaccg cagaaggtat
     3481 ctcccgaggc gcattcaata ccaaacaagg caacgatgtc tccaggaaca ccttcgttaa
     3541 tgtcttccat tgttgaggag ttcatgcgaa caagacgacc aaccttgaat tttttgcgtg
     3601 cacgggtgtt atacagttca tcaccctttt taagggatcc ctgatagaca cgaacatagg
     3661 taagctgacc gtattttccg tcttcgagtt taaagcccag ggcaacaatt ggggactttt
     3721 cgtcgcaaga caggagaacc tgctcttcgt ttttatcgag gtcaagagca tagttcttga
     3781 cttcggtggg gtcgggaagg tagcgggtaa caccgtcgag cagggactga atacccttgt
     3841 tcttgtaggc tgaacccaag aaaaccggaa caagctgttc tgcaagggtt cccttgcgga
     3901 tagctgcatg gatcatctct tcggtttcgg ttccttcaag gaaggcttcg gcaagttcgt
     3961 cggagaacat ggaggctgcg tctaccagtt cttcgcggta tttttgggcg tctgcctgaa
     4021 ggtgtgcggg gatttcggca atgcggattt cggttccgct ttcgccttca aagtacacag
     4081 ccttcatggt aaccaggtca acaatacctt cgagcttgtc ttcaagaccg atgggaagct
     4141 gggccatgta cgcattcaag ccgagttttt cccttagctg catacgtact tttaaggggt
     4201 tggcgccggt tcggtcgcat ttgttgacaa aggcaatacg ggggacatga tagcgtttga
     4261 gctgacggtc taccgtaatt gactgggatt gaacaccacc aactgagcac aatacgagga
     4321 ttgcgccgtc aagaacgcgt aatgagcgct caacttcgat ggtaaagtca acgtgtccgg
     4381 gggtatcgat aacgtttatc gtgtaatcct tccactgaac ctgggtcgag gctgatgcga
     4441 tggtaattcc tcgttcgcgc tcgagttcca tgttgtccat aacagcaccg acgccgtctt
     4501 taccgcgaac ctcatggata tgatggatct tgttgcagta aaaaaggatg cgttcggaaa
     4561 gcgtcgtctt tcccgagtcg atgtgggcgc tgataccgat attcctcatt ttggaaatat
     4621 caaaggccat aatcctgtac ctcactcatt attgattttg cgatatacgc aaatgttttc
     4681 ataaacatgt aatcaatttc agtataccgg aaataaaaag gtgttgcaag accagggatt
     4741 tggcccttat ttaaggcgca ttttgaaata aaaggataga agttgctcgt tggaaaggct
     4801 tgaataattc cattttacgt gtaatgcaag gtaccttttt ttctgttccc ggttagtgag
     4861 ggatgtgctt acggtgaatc gtgaatcagg ggggctttcc tggttttctt gcgtacgtac
     4921 aactgatgcc tcaagacgca cttggcgatg agccttaaaa acactggaaa cagtgatttt
     4981 tgtgttggta catcggtcag gctctatagt cgagtcggtc gtggtaccgc ttgcgccgag
     5041 ggtaagagag aggtttggat agatgcagtg atagttttta tacacaatgg acagatcgac
     5101 tgactcccct tcttttctcg cgccaatgtg aaagctgaaa tcccgattag cgatcttacc
     5161 ttgtacagat gtgtatgcat ttttttgcca ctcctggttc caggtatctg ttgagacggt
     5221 atcttcgcca taaagggctt gaaattcgag tctggttctt cctgctcccg gacagggaag
     5281 attgactgcc ggtgcaataa tccattgctt ttctatgggg tcatggttca gggaaaaatc
     5341 cagaaactcc ggatcctgtc ggtaccagga gccagcaatg gtaaaccatt tccagccggt
     5401 tctaacatca ctgtgaatag ccctctggtc gggtgttaac ggcgcggtat ttgtataccc
     5461 ggttgcgctc atggtaaaaa aaggaagaga gatggcaagt tgggccgcat gtaggtcaat
     5521 aggtactgca ggatagcttt ggattggtaa aaaccatgag tcgctctttg ttttgtccat
     5581 gggactgctt ccggaaaaga gtgaaaaaga gagtgtaacc tgggggggga ggggttcttg
     5641 caggacatga gaggcggcaa accaacgggg atgggcattg ggctgtagag gattagcgac
     5701 aagggcaatg cgcgtctgtg gtgtatgtat ctgtaggcca gtctgttcgg taagggtgcc
     5761 ggtcccgggt tgcacaagtg ggcgatcttg gggtgttatg gggcgatatc tagccgaggt
     5821 ctgttttctt accgggtttt ttacctgtga gggaagagcc gta
//
LOCUS       DAOQXQ010000017         5752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_14271_length_5752_cov_2.810075, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000017 DAOQXQ010000000
VERSION     DAOQXQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14271_length_5752_cov_2.810075"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKH81_00560"
     CDS             complement(<1..188)
                     /locus_tag="PKH81_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96568.1"
                     /translation="MTIAARNRFMQLATLASLLLAMIAVTSAIYILMNGLLSAERTGS
                     RFFSGLDSLFLAPFSARA"
     gene            282..962
                     /locus_tag="PKH81_00565"
     CDS             282..962
                     /locus_tag="PKH81_00565"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HNQ96569.1"
                     /translation="MRKCTIALILCLLAVAAYSGDVASFVSLGFTKDASVYAFGQHGV
                     TDASFASYSDVYYVDVAKNTFLPSGKFSAGPQASKGGHDSAVQFHELKQKAAAKMKNT
                     LIDEQNQGRPLYVQAAEEGRLKKIEFRDFETGRHFDVTMHTLTDGTLSRVASSFYLLV
                     RVTSSDGTVQSHTVGLPSYSRKGVKDYLIKRVITDTTGSSVVFVIEKEMYDTKGSSIR
                     YMVETLTL"
     gene            complement(952..1341)
                     /locus_tag="PKH81_00570"
     CDS             complement(952..1341)
                     /locus_tag="PKH81_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96570.1"
                     /translation="MKHTVRILSLNRPIEAPQTGEGPDGTRSITEYQSLITPKDLEPR
                     PEDHLVHGQTITTIPPGRYLFTQGKMPQDETTCEKLWRDAAEAMWLESLWLEETLKTD
                     RILVRILSEEEGSVYQLFREIEGPFTV"
     gene            1410..2450
                     /locus_tag="PKH81_00575"
     CDS             1410..2450
                     /locus_tag="PKH81_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96571.1"
                     /translation="MIRCVLAIMGSLILFTACFSCAVGSDTVSLWGTDAVSPSLVELC
                     PVSTLEVDAWFSAPVTVVSSRVQLDKAPERVIETVWALRDDGSAEEKTEGEGCCLRFA
                     MEEGPAVGEKAYLTALVEDEKGNSYSFCVPFTGYNERVAALAINEVRTEYDKPKVEYI
                     EFIVTRAGNIAGIEIYNAGNATVPYYEFPSAEVCEGDYIVWHLRSLEEGLVNETIDTD
                     ESSGTDATITGRDFWDTQTKAPLKKTNVIMVRERKGGIVMDALLVVESGKSEWPNDVL
                     AAAAEAAVSCGAWGPTSLVGDAVCSTGTTPTRTIGRNEFSSDTNTRGDWAICLTGKRS
                     PGVANPPFPASP"
     gene            2532..4856
                     /locus_tag="PKH81_00580"
     CDS             2532..4856
                     /locus_tag="PKH81_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNQ96572.1"
                     /translation="MAELLAPAGNPEALDAAINEGADAVYLGLKSFNARLRSTNFAWS
                     QFEATVESLHKRGKKVLVAVNTVLQESETERMYRFLSYLSRIGPDGIIVQDFGTLHLA
                     RSFFPSLRLHASTQMNIASARAANTMSRSGVSRVVLARELSLTEVRDIKARSSCELEV
                     FVHGALCVSESGLCLFSSYLGGKSANRGMCAQACRRLYTTGSGANARQGYFFSPHDLQ
                     LIDSIPDLVQAGIHSFKIEGRMKSAEYVGTVVSAYRYVLDNWEQDRKGAVETARRILA
                     NDFARKKTRYWYDSTLAENVLNPDQAGGTGIFLGKIDKQRETGEGHEVLLKGGSYDPD
                     EGDSVRVHKKDDSVRESHKIKKVVTDAEGKWIDIPEGFGRGDSVYLIQTKNMTKRYPH
                     VLPNDLSRYRSQPGADKLPPLPQAPLVESTQEVSAKKSKKIDPDFPDGVYVQVSTLDD
                     LFMILTDRPVRVIVEINQETIPALLGLPNEKTGAPSPVIPFAKREIILSLDPFSPQAQ
                     EDGLSTMLDELVENGYRHFIVNNPAHIGMLRNRGLKLISGPYLYAFNRWAVDWLKGQG
                     LQSLVCPIEISRDTLEASVDAHYRSQVFVTVFAYPALFRMRFKLPESYDFDFFQDRQG
                     GVFRALSTKEGSYVVPLVPFSIADKGAMLNKLGFTRHIIDISRSSIRRQDFRAVMDSY
                     RKGKILPEISRFNWKDGFYDPERIEEQKSMNERAALRQKKAGPSDKGQGRPGRGAKPG
                     ADMPHKEGRSHGRKKGDTGFSKSKGPGGRTPRSR"
     gene            complement(4849..>5752)
                     /locus_tag="PKH81_00585"
     CDS             complement(4849..>5752)
                     /locus_tag="PKH81_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96573.1"
                     /translation="LPALLVFKTDTGIRYFVLKEKVAPLAIKLCNDARKIIKDQLTRE
                     WYQALKSYRQENAMKSQVEFEKKLSELCKSSTPVLHALLTASFISLLASEGLEDTSGA
                     QGSFHLFSRGRLAPMSELLMLDRQEILTDTRIMLPFWYTIPVISAIITFLHRPKRAKV
                     KKHTGKTVQNATTKEEAAPTGKAAKEKRKEELREAAELIEKKLVPEGSDIETELTIHL
                     DSWNRTLDSQAKNNLTEDVNSLIRDYIRRIIRTIKAQSFDLPRIEGLARTLADTPSLA
                     KIPNRDALMGYIELYILKLVKGIT"
BASE COUNT         1458 a         1292 c         1512 g         1490 t
ORIGIN      
        1 cttgcgcgag cgctaaaggg agcaagaaaa agggaatcaa gaccagaaaa gaacctggac
       61 ccggtgcgtt cggcagataa taagccgttc atcaagatgt atatggcaga ggtgaccgca
      121 atcatggcca gaaggagaga tgccagagtt gctaattgca taaaacgatt acgggccgca
      181 atggtcataa gaaaataata gcatcaaaaa ggatttaagg gaatctcaag tgagggcagg
      241 ttttggtcga tacttaaata tcgaatatcc gggagatagg tatgagaaaa tgcacaatcg
      301 ccttaattct ttgtctgttg gccgttgccg cctattcagg cgatgtagcc agttttgtca
      361 gtttgggttt tactaaagac gcctctgtgt acgcgtttgg acagcatggg gttaccgatg
      421 ccagctttgc gtcctattca gatgtgtact atgtagatgt tgccaagaat acatttcttc
      481 cttccgggaa gttttcggca ggtccacagg ccagtaaggg cggccatgat tcggctgtac
      541 agtttcatga gttgaagcaa aaagccgcgg ccaagatgaa aaacaccctt attgatgaac
      601 aaaaccaggg caggcccctg tatgttcagg ctgcagaaga aggtcggctc aagaagattg
      661 aattccgtga ttttgaaact gggcgacact ttgatgtgac catgcatacc cttaccgacg
      721 ggacgctctc gcgcgtggcc tcatcatttt atctgcttgt cagggttacg tcttctgatg
      781 gaacagtaca atcacatacc gttgggcttc catcctattc ccgtaagggc gttaaggatt
      841 atctgataaa gcgtgttatt accgatacca caggatcgtc cgtggtcttt gttatcgaaa
      901 aagaaatgta cgataccaag ggtagttcca ttcgctatat ggtcgaaacc cttacactgt
      961 aaaaggccct tcaatctccc tgaacaactg atataccgaa ccttcctcct cggacaggat
     1021 tcttacaaga atcctgtctg tttttagtgt ctcttccagc cacaaagatt caagccacat
     1081 agcctctgcc gcgtctctcc agagcttttc gcaggtagtc tcatcctggg gcatcttgcc
     1141 ctgggtgaac agataccttc cgggagggat agtggtaatc gtttgaccgt ggaccaggtg
     1201 gtcttctggc cgtggttcaa gatctttagg agtaataagc gattgatatt ctgtaatgga
     1261 tctggttccg tcaggccctt cgcctgtctg tggcgcttct ataggacggt tcaaacttaa
     1321 tatacgaacg gtgtgcttca tgatctcata ctatcaaaaa aacagccaaa gggcgatatc
     1381 cgggatacaa aacaggtaag gaggtcctga tgattcgttg tgtattggcc attatgggct
     1441 cgttgattct atttaccgct tgtttctcat gtgctgtggg cagtgatact gtttcccttt
     1501 ggggtactga tgcggttagt ccgtctctgg ttgaactgtg cccggtgtct accctcgaag
     1561 ttgacgcctg gtttagcgcg ccggttacgg tagtttcctc ccgggttcag ctcgataagg
     1621 cgcctgagcg ggtaatcgag acggtatggg ccttgaggga cgatgggagc gcagaggaaa
     1681 aaactgaggg agagggttgt tgcctccggt ttgccatgga agaagggccc gcggttggcg
     1741 agaaggcgta tttaactgca cttgtagagg acgagaaggg caacagctac tcattttgtg
     1801 tgccgtttac cggatataac gagcgggttg cagccctggc gattaacgag gtgcgaacag
     1861 aatatgacaa gccaaaggtt gaatatattg agtttatcgt aacaagggct ggaaacattg
     1921 ccggcatcga aatatataac gcgggtaatg ccactgtgcc ttattatgag tttccttcag
     1981 ctgaagtatg cgagggcgac tatattgttt ggcaccttag atcccttgag gaagggcttg
     2041 ttaatgaaac aattgatacg gatgaatcga gtggtactga cgcgacgata actggaagag
     2101 atttttggga tacccaaacc aaggccccgc ttaaaaagac caatgtaatc atggttcggg
     2161 agcgaaaggg ggggattgtt atggacgcac tcttagtagt cgagtcgggt aagagcgaat
     2221 ggcccaatga tgtccttgcc gcggcggcag aggctgcagt gtcctgtggg gcatggggcc
     2281 ccacttccct tgtgggagat gcagtgtgtt caacgggaac aacaccgacg cgcactatcg
     2341 ggcgaaatga gttctccagc gatactaata cgcgaggaga ttgggctatc tgcctgacgg
     2401 ggaaacgaag tcccggtgtg gcgaatcctc cttttcccgc ctctccctga aaccttgtcc
     2461 ggggatctga gtgactgccc atttgcataa tggaagaaat aaggtactat acagcttgag
     2521 gggaggcgct catggcagaa ctattggctc cggccggtaa tccggaagct ctggacgcgg
     2581 cgattaacga aggagcggat gcggtatatc ttggccttaa aagctttaac gcccgtctca
     2641 ggtccaccaa tttcgcctgg tcccagtttg aagcaaccgt agaatctctc cacaaacgcg
     2701 gaaagaaggt gttggtagcg gtgaacaccg tactgcagga aagtgaaaca gaacgaatgt
     2761 accggttcct ttcttatttg tcccgaattg gcccggacgg aataatcgta caggattttg
     2821 gcactcttca tcttgcgcgc agttttttcc cgtctttgcg cctacatgca tcaacccaga
     2881 tgaatatagc aagtgcgcga gcggctaata ccatgagtag atcaggggtg tcccgtgttg
     2941 ttcttgcacg tgaactttca cttaccgagg ttcgggatat caaggcgcgt agctcttgtg
     3001 aactcgaggt ttttgtccat ggggctctgt gtgtcagcga atcaggactg tgcctttttt
     3061 ccagctatct tggagggaag tccgcaaaca ggggaatgtg tgcccaggca tgcaggcggt
     3121 tatataccac tggctctgga gctaacgccc gacagggata tttcttttct ccccacgatt
     3181 tacaactcat cgacagcatt ccagacttgg tacaagcagg gattcattct ttcaaaatag
     3241 aagggcgtat gaaaagcgcc gagtacgttg gtaccgtagt ctctgcctat cggtatgtcc
     3301 ttgataactg ggaacaggat cgaaaaggcg ctgtggagac tgcacgacgc atattggcaa
     3361 atgattttgc ccgcaaaaaa acccgatatt ggtatgattc aacgctggcc gaaaacgtat
     3421 taaaccccga tcaggcgggt ggaaccggaa tattcctggg aaaaattgat aaacagcgag
     3481 aaacgggaga aggtcatgaa gttctcctta agggtggctc ttacgatccg gatgagggag
     3541 actctgttcg cgttcataaa aaagacgact cggtacgaga aagccacaag ataaagaaag
     3601 ttgttaccga tgcagaggga aaatggattg atattcccga aggatttggc cggggagatt
     3661 cggtatatct tattcaaaca aagaacatga ccaagcgata tccccatgtg ctgccgaacg
     3721 atctctcccg ctatcgcagc caacccggtg ccgataaact gccgccctta cctcaagctc
     3781 ccctggttga atccacacag gaagtcagcg cgaaaaaatc aaaaaagatt gatcctgact
     3841 ttcctgatgg cgtctatgta caggtttcca ccctggacga tctttttatg attcttaccg
     3901 accggccggt tcgggttatt gtagaaatca atcaggagac gataccagca ttactcggat
     3961 taccgaatga aaaaactggc gctccttctc cggtaattcc ctttgccaag cgagaaatta
     4021 tactttccct tgaccccttt tctccccagg cccaagagga tggcctatcg actatgctcg
     4081 atgaacttgt agaaaatgga taccgtcatt ttatcgtcaa taatcccgca cacattggaa
     4141 tgctcagaaa tcgtggactc aaattgattt ccggaccgta tctctacgcc tttaaccgat
     4201 gggcggtaga ctggcttaag ggacaggggc ttcaatccct ggtttgtcct atagaaattt
     4261 cgcgtgatac ccttgaagct tccgtagatg ctcattatcg aagtcaggtt tttgttaccg
     4321 tttttgccta tcctgcttta ttccgcatga gattcaagtt gccagaaagt tatgattttg
     4381 atttttttca ggatcgccag ggcggggtgt ttcgcgcgct atccaccaaa gagggttcgt
     4441 atgtggttcc ccttgtaccc ttttctatcg cggataaggg agcgatgctt aacaaacttg
     4501 gatttactcg acacatcatc gatatctcgc gatcttctat cagaagacaa gacttccggg
     4561 cggtcatgga ctcctatcgt aagggaaaga tattacccga gatttcgcga tttaactgga
     4621 aagacgggtt ttatgatcct gagcgcatcg aagagcaaaa atccatgaat gaacgagccg
     4681 cactcaggca aaaaaaagct ggcccatcag ataaaggaca aggtcgtcca ggaagggggg
     4741 ccaagcctgg tgccgatatg ccacacaagg aagggcgaag ccatggcagg aaaaagggtg
     4801 acaccggctt ttcaaaaagc aagggccccg gtggaagaac gcctcgctct aggtaatacc
     4861 tttaaccagt tttagaatat acaattcgat atatcccata agcgcgtcgc gattcggtat
     4921 cttggcaagg cttggggtgt ctgcaagcgt gcgcgcaagc ccttcgatgc ggggcaggtc
     4981 gaaactttgc gccttgatcg tgcggatgat gcgcctgata taatccctga taagggagtt
     5041 aacgtcttct gtcaggttgt tttttgcctg agaatcaagg gttctattcc atgaatcaag
     5101 atggattgtt aattctgttt cgatatcgga tccctcgggg accagtttct tttctataag
     5161 ttcagcggct tcccgtaatt cttccttgcg cttttcttta gcagccttac ctgttggagc
     5221 ggcttcttct ttggttgtag cgttttgcac tgtctttccg gtgtgctttt taaccttggc
     5281 tcgtttaggt cgatgaagaa aggtaataat cgccgaaata acgggaatgg tgtaccagaa
     5341 gggaagcata attctggtgt cggtaagaat ttcctggcga tcgagcatga gtaactcgct
     5401 cattggggca aggcggccac gggaaaaaag atgaaaggaa ccctgagcgc cagaggtatc
     5461 ctcaagaccc tccgaggcca acagcgatat aaacgaagcg gtgagcagtg catggagaac
     5521 tggggtggag ctcttgcaga gctctgacag ttttttttca aattccactt ggctcttcat
     5581 cgcgttttcc tggcggtagc tttttagagc ttggtaccat tcacgcgtta actgatcctt
     5641 aatgattttt ctggcgtcat tacagagttt aatagcgagt ggggctacct tttccttgag
     5701 tacaaaatag cgaataccag tatccgtttt aaagacgagc aaggcgggga gt
//
LOCUS       DAOQXQ010000018         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_14369_length_5713_cov_2.613998, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000018 DAOQXQ010000000
VERSION     DAOQXQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14369_length_5713_cov_2.613998"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKH81_00590"
     CDS             complement(<1..1441)
                     /locus_tag="PKH81_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ96574.1"
                     /translation="MNTSRRIKPVILKVAVSLMLFLAINIPSFAENSGEPATRDIYEA
                     IRANDIRSLRLLLEMDNVEYYNHSEAYPLLYAIRVSNTQAVQILLPYYSNTTLIKIDP
                     FDENSKTILEEAESQNSPEIRRLIYNRVYSELNQDENIELLYLYLISDFEAVRQGVIS
                     KKIDIKNRKKHQKSLLHLCADKRDYNNRWFPIDVYLNEDNKVKLARFLIDQGIDLDLV
                     DDSRDTALHMAIEYGYNALAALLIEAGAAVNGTDQTSRSPLEQALQYKNTIICEKLVK
                     AGANLNEMMDYRRSLLAKSIDDEQFEFAKILIHGGADVNAKGEKGYSPLYEACDESNL
                     ELCTLLLEKGADVNSKNGKYDTPPLPNAIYPDNYELVELLLSHGADANGKDSDGQNAL
                     FNISSTNDMRIVQILLDSGADVNALDCNGSTPVLYAAGFGRLDQLELFTKYKGNFLVK
                     DNEGNTAIHAAVKGAVTKEVGLSDFSFLSG"
     gene            1613..3037
                     /locus_tag="PKH81_00595"
     CDS             1613..3037
                     /locus_tag="PKH81_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNQ96575.1"
                     /translation="MKSKRSDMVAVSIPGERYTPFALAKKLGARAILESASFTHGRDR
                     YSLILATEAFRIKQDEGGVSFLIDGQTIPYSGGDILDAQMEIAAQNVLPPDGIPLPGA
                     GIGFLGYEFCARCDTITLASQKDTLSIPESEFIVAHVYLVFDHFTDTIHIFGLNYEQH
                     EIDLHAAVRDIQSRLGDLDFSYLAPPERSSPSVVITDEAASKVEFTAGVNAIKDHIVK
                     GNLLQAVMSRRLELECEEGALEVYRRLRSRSPSPYLFFLDFGSYQLVGASPESLVRVR
                     DSVASIRPIAGTCRRGKNAIEDEELKKKLLDDPKERAEHLMLVDLARNDLGRVCIPGS
                     VRLSRTMDVELFSHVMHIVSDVVGQTGNGSSGGKSALGIDVLRASFPAGTVSGAPKIR
                     AIEILSSLETVRRGFYAGAVGYVEPDGDLDFCIAIRCALKKGNRWVLHAGAGIVHASD
                     SEREWEETNEKLAAMRTVLEGENK"
     gene            3034..4626
                     /locus_tag="PKH81_00600"
     CDS             3034..4626
                     /locus_tag="PKH81_00600"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component
                     II/anthranilate phosphoribosyltransferase"
                     /protein_id="HNQ96576.1"
                     /translation="MIVIIDNYDSFTYNVYQYFARLSSEEIRVIRNNEITIQELDALS
                     PSRLVISPGPGRPSEAGISIAAIRHFAGKIPILGICLGHQAIGEAFGGKIVGAKFIKH
                     GIAEEISLDGKGVFRTIGNKGVFTRYHSLVIEEESLPAELEVTARAQDGDIMGVRHRE
                     LDIEGVQFHPESIAATQGEALIKAFLSYRREGLSFSATLSSLMAGQSMSRETAELFME
                     DLTEGSLDERQTSAILTALSAKGPAAEEIAGCAAVLCRKKKNVPITASLTDIVGTGGD
                     GLGSFNISSMAALVAAACGIPIAKHGNRAVSSKSGSADFYEALGIKIDVSADKTAQII
                     NKTGFGFLYAPVYHSAMRFAAPVRKLLGVKTIMNLVGPLSNPAGAAYQMLGVYHPSLM
                     KPVAQASKMLGSERVMVVSSRDGMDEISPCDLTDVIEIDAEGKEKTFTVDPAALGVPS
                     CTMADLLGGDAQENVRLAREILSGGGRPGIRHAVALNAGATLYISGKTVSIEEGYHLA
                     LDALADGRVAAKIEEVREASHG"
     gene            4619..>5713
                     /locus_tag="PKH81_00605"
     CDS             4619..>5713
                     /locus_tag="PKH81_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol phosphate
                     synthase/phosphoribosylanthranilate isomerase"
                     /protein_id="HNQ96577.1"
                     /translation="MADILDQIIARRKEDYERLGPTFGSVIPQNRERAVCSFLKENGA
                     ILEIKRASPSKGDIAPDLDPVALARSYGEAGARNVSVLTEMRYFKGSLDDLVLVSSRF
                     PALSFLRKDFILYEDEIEVSFRAGADAVLLIARILDEKTLRSLAARCRSFGMTPFIEV
                     REGEDVAKLTAAAKDGFLVAGVNSRDLATFRTDLLVPALLRTSLPCRTIFESGARNEG
                     TCRFARQIGFDGILIGEAVAKNPQAAAGLVKSFLSAKPDWVGHFWRQIARRKNERRPL
                     VKICGFTRVEDALFAASLGADVLGFVFAPSPRETKVSCVRETSAALDKLYGTATSRST
                     LPAPTTERPLMVGVVVDAVSPQAENAFALVC"
BASE COUNT         1261 a         1449 c         1497 g         1506 t
ORIGIN      
        1 gacctgacaa aaaggaaaaa tcagacaatc caacctcttt ggtaacagct cctttaacgg
       61 ctgcatgaat agccgtgttt ccctcattgt cctttaccaa aaaattcccc ttgtactttg
      121 taaagagttc aagctggtca agtcgtccaa agccagcagc atacagtacc ggggtggagc
      181 cgttgcaatc tagtgcattt acgtcggcac cactgtcaag aaggatctgt acaatgcgca
      241 tgtcgttagt agaactgatg ttgaaaaggg cattttggcc gtcagagtcc ttgccatttg
      301 catccgcccc atgcgacaga agaagctcta ccagttcata gttatctgga tatatcgcat
      361 taggaagtgg tggggtatca tacttgccgt tttttgagtt tacatcagca cccttttcta
      421 gcaataaggt gcaaagctcc agattgcttt catcacatgc ttcataaagg ggtgagtaac
      481 ccttttcgcc tttcgcgtta acgtcagctc ctccgtgaat aaggatcttg gcaaattcaa
      541 attgctcatc atctatactt tttgctaata gtgagcgtct gtaatccatc atttcgttaa
      601 gattcgcgcc tgccttgacg agtttttcac agatgattgt gtttttatat tgaagggcct
      661 gttccagggg ggaccgtgag gtttggtctg ttccattaac ggctgctcct gcttcaataa
      721 gcagggcagc aagtgcatta tatccatatt ctattgccat gtgtaaggct gtatcccttg
      781 agtcgtcaac cagatcaaga tcaatgccct ggtcgattaa aaaacgcgca agcttgactt
      841 tattgtcctc gttcaggtac acgtcaatcg gaaaccagcg attgttataa tcccttttat
      901 ctgcacacag gtggagcagt gatttttggt gtttcttgcg attttttatg tctatttttt
      961 ttgagataac tccctgccgt accgcttcga agtcgcttat caagtacaga tacagaagtt
     1021 cgatattttc gtcctgattc agttccgagt atactcgatt atatatgagt cttcgtattt
     1081 cgggactgtt ctggctttcc gcttcttcca gaatcgtttt actgttctcg tcaaacgggt
     1141 caatcttgat aagcgttgta tttgagtaat aaggcagtaa tatctgaacc gcctgtgtgt
     1201 ttgaaactct aatggcgtat aacaggggat acgcttcact gtggttatag tactcaacat
     1261 tatccatttc cagtaaaagc cttagggacc gtatgtcatt tgcgcgtatt gcttcgtata
     1321 tgtcccgggt agcaggttcg cccgaatttt ccgcaaagga ggggatattg atagcaagga
     1381 acagcatcaa agaaacagcc acttttaaaa tgacgggttt aatgcgcctg gaagtattca
     1441 tgttcttttt cctcgttttt gttgtctgtt tcttattaaa ccctgttcct ttgggccggt
     1501 caaggaataa ccggatagtt ttttatctgt ttctattgac agaaacgcgt gctcccggta
     1561 gactgacatt gttactataa atagtaacaa ggcttaccaa ggagaaaacg ggatgaagtc
     1621 aaaaagaagc gacatggttg ctgtatcaat tccgggggaa cgctacaccc cctttgccct
     1681 tgccaaaaaa ctgggagctc gcgccatttt ggagtctgcc tcctttactc atgggcggga
     1741 ccgctactca ctaatccttg ctaccgaggc gttcaggata aaacaggacg aaggcggggt
     1801 gtcttttcta atagatggtc aaaccatacc ctattcaggt ggggatattt tagacgccca
     1861 gatggagatt gccgctcaaa acgtgcttcc ccccgacgga atccccctgc ccggtgccgg
     1921 tatcggtttt ttagggtatg agttttgcgc gcgctgcgac acgattaccc ttgcaagcca
     1981 aaaggatacc ctgtccattc ccgaaagcga gtttatcgtc gctcatgtgt acctcgtttt
     2041 tgaccatttt accgacacta tccacatttt tggacttaac tacgaacaac acgaaattga
     2101 cctccatgca gccgttcgcg atattcaatc ccggctgggg gatcttgatt tttcgtatct
     2161 tgcgccgccc gagcgcagta gccccagtgt ggttattacc gacgaggctg cttccaaggt
     2221 agagtttacc gcgggggtta acgcgataaa ggatcatatc gttaagggca acttgctcca
     2281 ggcggttatg tcccggcgtc ttgaacttga gtgcgaggaa ggcgctcttg aggtgtatcg
     2341 ccgtcttcgc tcccgctccc catctcccta cttatttttc cttgattttg gctcgtatca
     2401 gttagtgggc gcttcccccg aaagcctggt gcgtgtccgc gactctgtcg cttccattcg
     2461 gcccattgcg ggaacctgcc ggcgtggaaa aaatgccata gaggatgaag agcttaaaaa
     2521 gaagctgctg gacgatccca aggagcgggc cgaacacctg atgctcgttg accttgcccg
     2581 taacgatctt ggccgcgtgt gcatacccgg ctctgtccgc ctctcccgta ccatggatgt
     2641 ggaacttttt agccacgtaa tgcatatcgt ttcggacgtg gtcggtcaaa ccggcaatgg
     2701 gtcttccggt ggaaaaagcg cgcttggtat tgatgtactg cgcgcctcct ttccggcggg
     2761 cacagtaagc ggcgctccca aaattcgcgc catagaaatt ctttcttccc tggaaactgt
     2821 tcgccgtgga ttttacgccg gcgcggtcgg gtatgtagag cctgatggag accttgattt
     2881 ttgtatcgcg attcgatgtg cgcttaaaaa ggggaatcgc tgggtcttgc acgcgggggc
     2941 gggaatcgtt catgcgtcgg acagcgaacg ggaatgggaa gaaaccaacg aaaaactcgc
     3001 ggctatgcgc acagtacttg agggagaaaa caaatgatcg tcataatcga caactacgat
     3061 tcctttacct ataacgtcta tcagtatttt gcccggcttt cgtcggaaga gattcgggta
     3121 attcgcaata acgaaattac tatccaggag cttgacgccc tttcgccctc ccggcttgtt
     3181 atctctcccg gccccgggcg gccctccgaa gcggggatca gtatcgcggc tatccgccac
     3241 tttgccggaa agattcccat actcggcatt tgccttggac atcaggccat tggcgaggcc
     3301 tttggcggga agattgtggg tgccaagttt atcaagcacg gtattgccga ggaaatttcc
     3361 ctggacggaa agggtgtttt ccgcaccatc gggaataagg gcgtgtttac ccgatatcac
     3421 agtctggtca tagaagaaga gagtttaccg gcggagcttg aggttactgc ccgcgcacag
     3481 gatggcgaca taatgggtgt tcgccaccgt gagcttgata tagaaggcgt gcagtttcat
     3541 cctgagtcca tcgcggcaac tcagggcgaa gcccttatca aggcgttcct ttcgtatcgc
     3601 cgggaagggc tttcctttag cgcgactctt tcatccctta tggccggtca gagcatgagc
     3661 cgcgaaaccg cggagctttt tatggaagac cttaccgagg gctcgctcga cgagcggcaa
     3721 acctcggcta tcctgaccgc tctatcggct aaagggcccg cggccgagga gattgccggc
     3781 tgcgctgctg tgttgtgccg taaaaaaaag aacgtaccca ttaccgcctc tcttaccgat
     3841 atcgtcggaa ctggtggtga cgggctgggg agttttaata tcagttccat ggctgccctg
     3901 gtggcagccg cctgcggcat tcccattgcc aagcacggga accgcgccgt ctccagtaag
     3961 tccggttccg cggattttta cgaagccctt ggtatcaaga ttgatgtaag cgcggataaa
     4021 accgcacaga ttatcaacaa gaccggcttc ggctttttat acgcgccggt atatcacagt
     4081 gccatgcgtt ttgccgcccc ggttcgaaaa ctattagggg taaagacaat catgaacctc
     4141 gtcggtccgc tatccaatcc cgcgggcgca gcgtatcaaa tgctgggggt gtatcacccc
     4201 tcccttatga agcccgtggc ccaggcgtcc aagatgcttg gctccgagcg ggttatggtg
     4261 gtgtcaagtc gcgatggtat ggacgaaata tctccctgcg atcttaccga tgttatcgag
     4321 attgacgcgg aggggaaaga gaaaaccttt accgtggacc ccgctgccct tggagttcct
     4381 tcatgcacga tggcagactt gctcggtggc gacgcgcagg aaaacgtgcg ccttgcccgg
     4441 gaaatcctct ctggcggcgg tcggcctggc atccgccatg cggtagccct aaacgcgggg
     4501 gcgacgctct atataagcgg aaagaccgta tctatagaag aaggatatca cttggctctg
     4561 gacgcccttg cagatggccg cgtcgcagcc aagatagaag aagtgcggga ggccagtcat
     4621 ggctgatatc cttgaccaga ttatcgctcg gcgtaaagag gactatgagc ggttaggccc
     4681 aacctttggt tctgtgattc cgcaaaaccg cgagcgggca gtgtgttcct ttttaaagga
     4741 gaacggggca attctggaaa taaagcgagc ttctccctct aaaggagaca tcgccccaga
     4801 ccttgaccct gttgccctgg ctcgttccta tggagaggcc ggagcccgga atgtgtcggt
     4861 gttaacggag atgcgttatt ttaaggggag tctggacgac ttagtgcttg tctcttcccg
     4921 ctttccggcg ctttcttttt tacgcaagga ttttatcctc tacgaggacg agattgaagt
     4981 ttcctttcgc gccggggccg atgcggttct gttaatcgca cgaattctgg acgaaaaaac
     5041 ccttaggtcc cttgcggcac gatgccgctc ctttggcatg acccctttta tagaggttcg
     5101 cgagggagaa gatgtcgcca agcttaccgc cgctgcaaag gacggttttt tggtggccgg
     5161 tgttaactct cgagaccttg ctacctttcg caccgacttg ctggtgccag cgctcttgcg
     5221 taccagtttg ccctgtcgta ccatctttga atccggggcc cgtaacgagg gcacctgccg
     5281 ctttgcccgt caaatcggtt ttgacggcat tttaatcggc gaggctgtcg caaagaaccc
     5341 acaggcagct gccggtttgg taaagtcttt tctttctgcc aagcccgatt gggtcggaca
     5401 cttttggagg caaattgcca ggcggaaaaa cgagcggcgc ccccttgtta aaatatgcgg
     5461 gtttacccgt gtggaagatg cgctgttcgc ggcctctctt ggcgcggatg tgcttggctt
     5521 tgtgtttgca ccaagccccc gcgagacaaa ggtttcctgc gtgcgggaaa cctctgccgc
     5581 gttggacaag ctatacggta ctgctacttc acgatccacg cttcccgcac caacgaccga
     5641 gcggccgctt atggtgggcg tggtagtgga tgcagtgagc ccacaggcag aaaacgcctt
     5701 tgccctggtg tgc
//
LOCUS       DAOQXQ010000019         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_14410_length_5704_cov_2.853425, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000019 DAOQXQ010000000
VERSION     DAOQXQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14410_length_5704_cov_2.853425"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKH81_00610"
     CDS             complement(<1..784)
                     /locus_tag="PKH81_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNQ96578.1"
                     /translation="MLALSKWKNIPLSCTPMSNKTLVVSIPKFPSSPFTSVFPVKISY
                     CPEYVVDNSINPAHNYSPTIFITHQQQSISYQHLPRGLYTPVSHRIVISDYLGLCNWK
                     APLIHDSLPPPFTINPIPLVCKTPPLPIGTTGILRGSSTFLRSDNLYEIRPYHTGDDP
                     RKINWKVYAHTGDLSVREGELLPPPSSEYIFIINQHEPRKNNSSSYKDASERFDILLS
                     KVAHLALSLLDHGHLITIQFFGEHFYSTTIHPMDEQAHAKILD"
     gene            complement(942..1985)
                     /locus_tag="PKH81_00615"
     CDS             complement(942..1985)
                     /locus_tag="PKH81_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNQ96579.1"
                     /translation="MSYSLKKPTRMVYFLDMTTAQTADNLLKGLSSVIRGKNHLLELY
                     ISSFLSGGHVLLDDVPGTGKTTLAKALSRLVGISSNTTLSPATFHRIQFTPDLLPYDI
                     TGVDIFNPKEQRFEFIPGPVFSDILLADEINRTTPKVQSALLEVMAEQQVSSGGVTRK
                     VSDLFFVVATQNPVETEGTYPLPAAQLDRFMLKLSLGYPDKESELSILREDPSQTILP
                     DLVPVVSCDDIRLSRKEQATVYCHPALESFIIAIVRITRDHPQLSLGASPRGALQLLH
                     ASRTLALMRGRTWIEDIDISDLAPAVLAHRLIPRDASLNTVELIRNITIQVCAEISHT
                     TDWTKDVAHKEHA"
     gene            2040..3023
                     /locus_tag="PKH81_00620"
     CDS             2040..3023
                     /locus_tag="PKH81_00620"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ96580.1"
                     /translation="MAVILCAVGGISVFFLSAGYVVYGAMMCSPRKRGTTTAAQEKGL
                     NSGVFSHIRDERIMEPLLASKQRWQEGLTQGLIERLSVMSKKGVFLSGYYYSAEGEAR
                     KLVVLVHGILDSAAGLGYLAEEYHKRGWDVLNLDLRGHGESDGTRYTMGVREAEDLAI
                     WIDLIQKKWPKKEVLIHGISLGGATALMYSGYPEVAESPVKGVVSDSSFGSYKEAAKT
                     LVTAVVRVGWIARGIVCCMSFFSILHTGIGFGKMRPEEAVTRKKIPVLAIHGEADALV
                     PLATISVLFSGIQGLLDEVLIVPDAPHIGAYFYKPEAYMNHIEAFATRSMG"
     gene            3020..5056
                     /locus_tag="PKH81_00625"
     CDS             3020..5056
                     /locus_tag="PKH81_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96581.1"
                     /translation="MRKKNVLGGLFLLIFFGFRLCGEDASLPINRISLFSSGVGYYEH
                     RGSVSGPTGMDIMLPVNAINDALKSLVIHDNASKNPSVSYPSEDTLSRTLKSLSVDLT
                     LSQSLADILGGLRGAEVIVKAPEELSGRIVGVEFVTQNGSEADNSATGERVHYKRASL
                     TLIGRDGLKTISLSEISSLTFTDPDITRDFHRALDLIQTERDSESRLLRLDLPGKGER
                     EVRVSYVIPSPVWKVSYRLDLHTKKPVLQGWAIVDNAGDTDWEQVRLTLVSGRPVSFI
                     QELYPPLYVPRPIIPLSIAGMAKAETYDSGWNAAPDEYAESEMSYSDSYPLAKAAAPR
                     LVNPARSDSGVSTGVVQTASAEAAGELFLYTLPEPVSLARRQSAMVPFVEAEVKAERV
                     SVFSGQKAFNGGIQNPNLCVLLENTSGLKLPPGPITIFDGGSYAGDALVEFLGEKDKR
                     LIAYGEDLAVQGYSRYSGSRRFTGITVSAGVMTIARKTISEWSYTLKNNSDAERLIWV
                     EHPISSTMTLREPREYAEKTDAVYRFPIKLLSGKEGSITVVEEAPVYETIVLASLKED
                     VLLQYSTSGEIPAKVKAVLEKLVTLRQAEDTARQKLVGIEATRKDKIGEQDRIRQNLQ
                     AVGNATEQGRQYMLKMSALDSEIETLVGQIDIGRKTLREATSAVGSYISSLKID"
     assembly_gap    5177..5186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5187..>5704
                     /locus_tag="PKH81_00630"
     CDS             <5187..>5704
                     /locus_tag="PKH81_00630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ96582.1"
                     /translation="FDELQFSFLEKVLDRDGSVAPHRYLDIGCGTGTMLSAFSDRFAQ
                     VYGLDLDPSLLALAAQKMLPGEGKKVDLLEGDMRNLRGFFPEEEFSFITCLGNTLVHI
                     TSSAAIIDFLSSVRSIMEGQGVFVFQIINYDRILDQGIRGLSPIVTEGISFERYYSLP
                     KDNGLIDFDTVL"
BASE COUNT         1611 a         1011 c         1411 g         1661 t
ORIGIN      
        1 aatctaaaat ctttgcatga gcttgttcat ccatagggtg tattgttgtt gaatagaagt
       61 gctcgccaaa gaactgaata gtaataaggt gtccatgatc gagaagggat agtgcaaggt
      121 gcgcaacttt agaaagcaag atatcgaagc gttctgatgc gtctttatat gatgaggagt
      181 tgttttttct aggttcatgt tgattaataa taaaaatgta ttctgaactt ggtggaggca
      241 gcaattcccc ttctcgtaca gaaaggtctc cggtatgagc atacaccttc cagtttatct
      301 ttcggggatc atctccggta tgataggggc gtatttcgta aagattgtct gaccgaagaa
      361 atgtacttga tccacgaaga atacctgttg tccctattgg aagcggcggt gttttgcaaa
      421 caagaggaat agggttaata gtaaaaggag gagggagcga atcgtgtatt aatggagctt
      481 tccagttaca caaacccaaa taatcagata tcactattct atgtgatacg ggtgtatata
      541 gacctcgggg aagatgttgg taagaaatag attgttgttg atgtgtaata aaaatagttg
      601 gcgaatagtt gtgcgccggg tttattgaat tgtcgaccac gtattcaggg caataagaga
      661 tttttacagg gaatacggaa gtaaatggtg atgaaggaaa tttcgggatg gaaactacaa
      721 gggttttgtt ggacataggt gtacaagaaa gtggaatatt tttccatttg gaaagagcta
      781 acaatgtaca cgtacatgaa tgaaggaatg ttataagcat acataaacct gcaagatttg
      841 ctgcaagttc tcctcgaatt aagcccaggg ataacaaaag taaagagata aaaatccaaa
      901 ttattggtaa ataagaccag gataggtaaa atcgccgggt tttatgcgtg ttctttatgc
      961 gcgacatctt ttgtccaatc cgttgtatga gatatttctg cacatacttg gatcgtgata
     1021 tttctgatta gttctactgt attaagggag gcatcccgag gaattagtcg atgggcaagg
     1081 acagcgggtg ccaaatcaga aatgtcaata tcttcaatcc atgttcgtcc tcgcatcagt
     1141 gcaagagtac gcgatgcatg taacagttgc agagcgccgc gaggagaagc tccaagtgaa
     1201 agctgaggat gatctcgggt tattcttaca atagcaataa taaatgattc taaagcagga
     1261 tgacaataaa ccgtggcttg ttcttttctg gataagcgaa tgtcatcaca tgatacaaca
     1321 ggtactaaat caggtagaat tgtctgtgaa gggtcttctc ttagaattga taactctgat
     1381 tctttgtcag gatatccaag gctgagttta agcataaatc gatcgagttg tgctgcaggt
     1441 aaaggatagg tcccttcggt ttcaaccgga ttctgagttg caacaacaaa gaaaagatca
     1501 cttaccttac gagtaacacc acctgatgaa acttgttgtt cagccattac ctcaagaagt
     1561 gcggattgga cctttggagt tgtacggttt atttcgtctg ctagaaggat atcgctgaac
     1621 acaggaccag gaataaactc aaaccgttgt tcttttggat taaagatatc aacacctgtt
     1681 atgtcgtaag ggagtagatc aggggtaaac tgtattcgat ggaatgttgc aggagagagg
     1741 gtagtattac tggaaatacc aacaagacga gacagggctt tagctaaagt agtttttcct
     1801 gttcctggaa cgtcatcaag taatacgtga ccgccagaaa gaaaagagga aatatatagt
     1861 tctagaaggt ggtttttacc acggattact gaagaaagac ctttgaggag gttgtccgca
     1921 gtttgagctg ttgtcatgtc taaaaagtat accatacgcg tgggtttttt taaagaataa
     1981 ctcatgctga ttgcagtttg aggaattacc gtatattctt tgccaatatg gaaatcttct
     2041 tggcggtaat actttgtgct gtgggtggta tctctgtttt tttcctttct gcaggatatg
     2101 tggtttatgg agccatgatg tgttctcccc gaaagcgagg aaccacaacc gcagcacagg
     2161 aaaaagggct taattccgga gtcttttcac atatacggga tgaacggatc atggagcctc
     2221 ttcttgcttc caagcaacgc tggcaggagg gccttacaca gggccttatt gaaagactta
     2281 gtgtaatgtc gaaaaagggt gtctttctta gtggatatta ttattccgca gaaggcgagg
     2341 ccagaaagct cgttgttctt gtccatggta tacttgattc tgctgctgga cttggttatt
     2401 tagctgagga gtaccataag agaggttggg atgtgttgaa tcttgactta aggggacacg
     2461 gagagagcga cgggacccgc tatacaatgg gcgttagaga agcagaggat cttgcgatat
     2521 ggattgattt gattcaaaaa aaatggccca agaaagaggt gttaattcat ggtatatcct
     2581 tgggtggagc cactgcttta atgtattccg gttatccaga ggtggcagaa tcccctgtta
     2641 agggagttgt ttccgattca tcgtttgggt cttacaaaga agccgccaaa accctggtta
     2701 ccgctgtggt acgtgttgga tggattgccc gggggattgt ttgctgtatg tctttttttt
     2761 cgatactaca tacaggaatc ggattcggta aaatgaggcc tgaagaggct gtaacacgta
     2821 aaaagattcc agttttggca attcatggtg aagcggatgc acttgttcct cttgcgacga
     2881 tttctgttct tttttccgga atccaaggct tattagacga ggttttgatt gtacctgacg
     2941 caccacacat aggggcatac ttttataaac ctgaagcata tatgaatcat atcgaggcat
     3001 ttgccacaag gagtatggga tgagaaaaaa aaatgttttg ggagggcttt tccttctgat
     3061 tttttttggt tttaggttat gtggagaaga tgcaagctta ccaattaatc gtatttctct
     3121 tttctcctct ggagtaggtt attatgaaca cagaggaagc gtatcgggtc caacaggtat
     3181 ggatatcatg cttccggtaa acgcgatcaa tgatgcgctt aaatccctgg ttattcatga
     3241 taatgcgtca aaaaatcctt cggtgagtta tccctcggaa gatacacttt cgcgaacact
     3301 taaaagtctt tctgttgatt taaccctttc acagagtctt gctgatattc tggggggatt
     3361 acgtggtgcg gaagttattg taaaggcccc agaggagctt agtggacgca tagtcggggt
     3421 tgaatttgtt acccaaaatg gctctgaagc agataacagt gcaactggag agcgcgtaca
     3481 ctataagagg gcaagcctta ccttgattgg gagggatggt ttaaagacaa tttctctttc
     3541 cgagatttcc tctttaacat ttaccgatcc agatataacg cgcgattttc atagagcctt
     3601 agatcttatt caaacggaaa gggatagtga atccaggctt ttacgtcttg atcttccagg
     3661 aaaaggagaa agagaggttc gggttagcta tgtaatacca tctcctgtat ggaaggtttc
     3721 atataggctc gatttacaca caaaaaaacc agtattacaa ggctgggcga ttgtagataa
     3781 cgcaggtgat actgattggg aacaggtaag actaacactt gtgagtggaa ggccggtgtc
     3841 ctttattcag gaattgtatc ctccgttata tgtaccgcgg ccaatcattc ctctgtccat
     3901 agcaggtatg gcaaaggcag aaacgtatga tagcggctgg aatgcggcac ctgatgaata
     3961 tgcagaaagt gagatgtctt attcagatag ctatccatta gctaaagcgg cagctccgcg
     4021 cttggttaat cccgctcgtt cagattccgg agtgtctaca ggggttgtgc aaacggcttc
     4081 tgcagaagca gcaggagagc tctttttata tacacttcct gaacctgttt ctcttgcacg
     4141 taggcaaagt gcaatggtcc cctttgttga agccgaagtg aaagctgagc gagtgtctgt
     4201 tttctctgga caaaaagcgt ttaatggcgg tattcaaaac cccaatctat gtgtgttatt
     4261 ggaaaatact tctggattaa agttgccccc aggaccgatc accatctttg atggaggtag
     4321 ttatgcaggg gatgcccttg tggaattttt aggagaaaag gataagcgac taatagccta
     4381 tggagaggat cttgctgttc aaggttattc acgctattca ggtagtagac gttttaccgg
     4441 aataactgtt tctgcaggag taatgaccat cgcaagaaaa acaatctctg agtggtcgta
     4501 tacactaaag aacaactctg atgcagaacg acttatttgg gttgagcatc ctataagtag
     4561 cacaatgacg ttaagagagc ctcgagaata tgcagaaaaa actgatgctg tatatcgatt
     4621 tccgataaag cttctttccg ggaaagaggg aagtatcacg gttgttgaag aagctccagt
     4681 atatgagact attgtccttg cctcgttaaa agaggatgta ttgctacagt attccacttc
     4741 cggagaaatt cctgcaaagg taaaagcagt acttgaaaaa ttggtaacac tacgacaggc
     4801 tgaagacaca gcacgacaaa aacttgtggg cattgaggct acaagaaaag ataaaatcgg
     4861 tgagcaagat cgaattagac aaaaccttca ggctgttggt aatgcaaccg aacaggggcg
     4921 acagtatatg ctcaagatgt cagctcttga ttcggagatc gagactctgg ttggtcaaat
     4981 cgatattggg cggaagacgc ttcgcgaggc gacaagcgct gttggctctt atatttcttc
     5041 gcttaagatc gactaatgtt tctgtctttg aactgttgac gcatctccct ttgtgcctga
     5101 tgattaaata agccggattc ggctttggga ggtgcactat gtcgttttat tcacgactgg
     5161 cagaacatta tgacttnnnn nnnnnntttg acgaacttca attttctttt ttagaaaaag
     5221 tccttgatcg ggatggttca gttgctccac accgatacct ggatataggg tgcggtacag
     5281 gaactatgct ttcggcgttc tcggatcgct ttgcacaggt atatgggctg gatcttgatc
     5341 cttctttatt agctcttgct gcccagaaga tgttaccggg cgagggaaaa aaggttgatt
     5401 tacttgaagg ggacatgcgg aatttacggg ggtttttccc ggaagaagag tttagtttta
     5461 taacctgtct tgggaatacc ctggtacata taaccagttc cgctgctatt attgattttt
     5521 tatcgtcagt tcggtctatt atggaaggtc aaggggtttt tgtttttcaa attattaact
     5581 atgaccgaat acttgatcag ggtattcgcg gcttatcccc aatagtgact gagggaatca
     5641 gttttgagcg gtattattcc ttaccaaagg acaatggact cattgatttt gatacagtac
     5701 ttaa
//
LOCUS       DAOQXQ010000020         5407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_15432_length_5407_cov_2.881166, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000020 DAOQXQ010000000
VERSION     DAOQXQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15432_length_5407_cov_2.881166"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..321)
                     /locus_tag="PKH81_00635"
     tRNA            complement(248..321)
                     /locus_tag="PKH81_00635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Ile,seq:cat)
     gene            438..881
                     /locus_tag="PKH81_00640"
     CDS             438..881
                     /locus_tag="PKH81_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ96583.1"
                     /translation="MILDKAETHRRNTRQRAALLELLTSVTCHPSAQWLYEQLAASCP
                     GMSLATVYRNLSLLAKEGKIRTLHCAGDTVRFDGDIGIHYHVVCSQCGAIVDIPGDTG
                     ESTRLCELAHRTSGYVVSDHSFNFYGICPDCQSGNHTVLKDHQFL"
     gene            913..1491
                     /locus_tag="PKH81_00645"
     CDS             913..1491
                     /locus_tag="PKH81_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNQ96584.1"
                     /translation="MKSIKDSQTEKNILAAFIGESQARNKYTFWASKAKEEGYIQIQQ
                     IFLETAEQEKEHAKRLFKFLEGGAEVAIPSGGNAGFIGTTLENLRQAASGENHEWQHM
                     YPDYAKIARSEGFDTVATVMENIAVAEKQHAKRYEAFAKHIEDGDMWVQEGPTTWRCL
                     NCGFIYVGKEAPKTCPACAHPQAYFERLVENW"
     gene            complement(1542..2681)
                     /locus_tag="PKH81_00650"
     CDS             complement(1542..2681)
                     /locus_tag="PKH81_00650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ96585.1"
                     /translation="MKEHDSASLRKAVQAFAGKGRKVVAGEGLQESFSFSGLLESFVH
                     LEFTEEEAIAHWKGILAHAALLTNDLGRKIGIHLALVDYFTNINHMMDSPMLVEVHVF
                     KQTERLAMVDGLTGVFNRRYMDIVLKKEFNRCDRYGKNLSLCLIDIDNFKNINDTHGH
                     IFGDEVLREISQLVKDVVREEDIVCRYGGEEFLVLLPETDEAGAEILAERIRMTMKGR
                     EFFTKYNITFSAGLATYPLSAMDMEGLVMAADRALYQAKFDGKDRAVSASPERRRFGR
                     YPQTWNLNIYPDSEARPITGVITQNVSLGGIQFECPVRYPVDCSIRLVFTSLDIEAPD
                     VEAQARITWAKKTMDGYTYGVSFVDTPEILEAKLETAIGALESIS"
     gene            complement(2754..3023)
                     /locus_tag="PKH81_00655"
     CDS             complement(2754..3023)
                     /locus_tag="PKH81_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HNQ96586.1"
                     /translation="MMADKQKVSRLVKTARGQLDVILKMIEDDRYCMDISTQLLAAAS
                     LIKKAQGEVMRAHLSHCVQDTLLAGDKEATKDKIDEILKILEKNM"
     gene            complement(3027..5300)
                     /locus_tag="PKH81_00660"
     CDS             complement(3027..5300)
                     /locus_tag="PKH81_00660"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ96587.1"
                     /translation="MIITTKFIIEGMTCAGCSATVERRVVALSGVEKADVNLIAKTMS
                     VRHDEDIVTAPEIIGAVQDAGYTATLVAPPSSDGSAPGFHHGPVLQKGEGMAVRLATS
                     LLFTIPLFYIAMGHMFHWPLPDFVHQNPLTFGLLQFLLLVPVLVVNRVFFVRGFKTLW
                     KRSPTMDSLIALGSSAAVVYGLYTLFAMSDSLFIGDTAKARDFAMTLYFESAAMILTL
                     ITLGHYLEDRAKSHTTDAISKLIRLRPDTATVERNGREELIPVEHIVIGDIVVVRPGQ
                     NIPVDGTVVEGYSTLDVSAITGESIPVEKKAGNQVYSATINLTGYLKLRADRVGNDTT
                     LAKIIALVEEASVSKAPISRLADRISSYFVPAVILIALITAAVWLLLGYGTAFAVSSA
                     ITVLVISCPCALGLATPTAIMVGMGKGAQSGILVKSAEALEIAHAVNTVVLDKTGTVT
                     AGKPVVTDVISVGELDDLELLALTASIEAPSEHPLSYAIVGEAKKSGLTITPVEKFTA
                     HPGKGVEAVIRGKRYFAGGPMLMAEHGVDVSVLMPRLSELGMQGKIPFCIGSESGPLG
                     IVGVADVIKSGSREAVSAFKALGMDVILLTGDNHYTARGIAAETGIDTVIPEMLPADK
                     ADYVKKLKSSGKTIAMVGDGINDAPALVCADVGIAIGAGTDIAIDSAGIVLMHSDLMD
                     AVGALRLGKAVIRNIRQNLFWAFIYNVLGIPIAAGVFYGAFGLTLNPMIAAGAMSLSS
                     ISVVLNALRLNLFKKEG"
BASE COUNT         1383 a         1358 c         1228 g         1438 t
ORIGIN      
        1 ttgcatcagt gtagttacgt tgaaatgttg aatgtggaga gccacgcttt ccggtaagtt
       61 cgttgtaaaa ctggacgtag aaaattccgc gatgagtctt gtacaagtaa aaaggacgca
      121 tacacagccc ctttcaagcc gagagtcagg cacattttgc cttaactctt gccttaaaat
      181 gtaaactatg gatgcgccct tctttgaagg gaagcacgtt attccttaca acgtaaggaa
      241 atctttttgg gcccacctgg acttgaacca gggaccgacg gattatgagt ccgcagctct
      301 aaccaactga gctataggcc cggacgtatg caaacctaac agtggaaata tagccgatta
      361 ggcgggaggc ggtcaagaga aggctatttt ggcctttttc ttgacagatt gattattgca
      421 tgataggctt attaagaatg attcttgata aagccgaaac tcatagacga aatacccgac
      481 aaagagctgc attgcttgag ctactcacct cggttacttg tcatccttca gcgcaatggc
      541 tttacgaaca gcttgcggcc tcttgccccg gaatgagcct tgcaacggta taccgtaatc
      601 tttctttgct tgccaaggaa ggaaagattc gcacccttca ttgcgccgga gatacggttc
      661 ggtttgacgg ggatatcggg attcattacc atgtggtatg ctcacagtgc ggggcgattg
      721 tggacattcc tggggataca ggcgagtcta ctcgtctttg tgagttagct catcgtacat
      781 ccgggtatgt tgtttcagat cactccttta atttttacgg gatttgcccc gactgtcagt
      841 cgggtaatca tacagtttta aaagaccatc aatttttgta atgcggctag gccgcggtac
      901 gtggaggtac acatgaagtc aattaaggat tcgcaaaccg aaaagaacat tcttgcggcg
      961 tttatcggcg aatctcaagc ccgcaacaaa tataccttct gggccagcaa ggccaaggaa
     1021 gagggataca ttcaaatcca gcagattttt ttggagactg cggagcaaga aaaggaacat
     1081 gccaagcgct tgttcaaatt tcttgaaggt ggcgccgagg ttgcaattcc atccggagga
     1141 aatgccggtt ttatcggtac tacgctagaa aatctgagac aagctgcctc tggagaaaac
     1201 catgaatggc aacacatgta tcccgattac gcaaagattg cccgctccga aggttttgat
     1261 acggtagcca ccgttatgga aaacattgcg gttgccgaaa aacagcatgc caagcgatac
     1321 gaagcctttg ctaaacacat tgaagacgga gacatgtggg ttcaagaagg tcctactacc
     1381 tggagatgcc ttaactgcgg ctttatctat gtaggcaagg aagctcccaa gacctgtccg
     1441 gcttgcgctc atcctcaagc atattttgaa cggcttgtgg agaactggta attcatcctg
     1501 tacattaaaa aaagctgtcc ggtttcccgg gcagcttttt tttagcttat ggactccagg
     1561 gcgcctatgg cggtttccaa ttttgcttcc aagatttccg gggtatccac aaaggacact
     1621 ccataagtat atccatccat ggttttttta gcccaggtta ttcgagcctg agcctctaca
     1681 tcgggggctt ctatatccaa actggtaaac acgagccgga ttgagcaatc gacgggataa
     1741 cgaacagggc attcaaactg gatgcctccc aaagatacgt tttgagtaat aactccggtt
     1801 atcggccgtg cttcgctatc gggatagata ttcaagttcc aggtttgtgg atatcgtcca
     1861 aatcttcttc tttcggggga tgcggatacc gctcgatcct ttccgtcaaa ctttgcttga
     1921 tataaggctc tatctgcagc catcacaagg ccttccatat ccatcgcgga taatggatat
     1981 gtggcaagcc ccgccgagaa ggttatgttg tatttggtaa aaaattcgcg tcctttcatc
     2041 gtcattctga ttctctcggc aaggatctcc gcacccgctt cgtcggtctc gggtaataac
     2101 accaggaact cttctccgcc atatcggcag acaatgtctt cctctcgcac aacgtctttt
     2161 accaattgag atatttctcg caagacttca tcaccaaaga tatgcccatg cgtatcattt
     2221 atgttcttaa aattatctat gtcgataaga cacagagaca ggttttttcc ataacggtcg
     2281 cagcggttaa attctttttt taatacgatg tccatgtagc gccggttaaa gacgccagtt
     2341 aacccgtcca ccatggcgag tcgttctgtt tgcttgaaca catgaacctc caccaacatg
     2401 ggagagtcca tcatatgatt gatattggta aaataatcga cgagcgctaa atgtatgccg
     2461 atttttcttc ctagatcatt ggttaacaat gctgcgtgcg cgagtatgcc tttccaatgg
     2521 gctatcgcct cttcttcggt aaactctaga tgtacaaacg attcaagcaa tcccgaaaag
     2581 gagaacgatt cctgtagccc ctcacccgca acgaccttgc gtccctttcc ggcaaaggct
     2641 tgaacggcct tgcgtaaaga cgcactatca tgctctttca tacagaaatt cctttcgact
     2701 tttcaaactt ttgtgcaatt atatagtctg gccggaaggt ttgtcgagca aaattacata
     2761 ttcttttcca gaattttcaa tatctcgtca attttatcct tggtagcttc tttatcccct
     2821 gctaacaagg tatcttggac acaatggcta agatgggcac gcatgacttc tccctgcgcc
     2881 ttctttatca gagaagccgc cgctaataac tgagtagata tatccatgca atagcgatcg
     2941 tcctctatca tcttgagtat tacatcgagt tgcccccgtg cggtcttaac cagccgggaa
     3001 accttttgct tgtctgccat catcggtcaa ccttcctttt taaataaatt cagtcttagg
     3061 gcattcagta ccacggatat tgagctcaag ctcatcgctc ctgcggctat catgggatta
     3121 agcgttaatc caaaggctcc gtaaaaaacc cctgcggcta taggtatgcc cagtacgtta
     3181 taaataaagg cccaaaacaa gttttgccga atatttctta ttaccgcctt cccaaggcgt
     3241 aaggctccaa cggcatccat caagtcgctg tgcattaata ctattcccgc tgagtctatc
     3301 gcgatatccg ttcccgctcc tatggcaatg cctacgtcgg cacagacgag ggcaggggca
     3361 tcattgatac cgtcaccaac catcgctatg gtttttcccg agctttttaa tttctttacg
     3421 taatctgcct tgtctgccgg aagcatctcc gggataacgg tgtctatgcc agtttccgcc
     3481 gcaattcctc tggctgtata gtggttatcc ccggttagca atatgacatc catacccagg
     3541 gctttaaaag cacttacggc ttcccgactc ccgctcttaa tgacatccgc aacacccact
     3601 atgccaagcg gaccgctctc cgatcctata caaaaaggaa tctttccctg cattcccaat
     3661 tcagacagtc gaggcataag cacgctgaca tccactccat gttcggccat cagcatgggg
     3721 ccccccgcaa agtagcgctt gccccggatt accgcttcca ctcctttacc gggatgggca
     3781 gtaaatttct ctactggcgt tatggttagt ccgctcttct ttgcttctcc cactatcgcg
     3841 taactaaggg gatgttcgga gggcgcttct atcgatgcgg taagtgcaag taattccaga
     3901 tcatcaagtt ctccaacaga gatcacatcg gttaccacag gcttccccgc ggtgacagtt
     3961 cctgttttat caagcactac ggtgtttact gcatgggcta tttccagggc ctccgctgat
     4021 ttaaccaata ttccgctctg tgcacctttt cccattccca ccattatggc agtaggggtg
     4081 gccaggccca gagcgcaagg acaggatata accaatacgg ttatcgcaga ggaaaccgca
     4141 aaggctgttc catatccaag taaaagccat acagctgcgg ttatcagggc aattagaata
     4201 acagcaggca caaagtacga gctgatacga tcggcgagac gagagatagg agctttagaa
     4261 accgaggctt cttctaccag tgcaattatt tttgcaagtg tcgtatcgtt ccctacacga
     4321 tcggcgcgaa gcttcaggta tccggttaaa ttaatcgtgg cgctgtatac ctgattccca
     4381 gccttctttt ccaccggtat gctttcgccc gttattgcag atacatcaag ggttgaataa
     4441 ccttccacaa ctgttccgtc caccggaata ttttgtcccg ggcgtaccac aacgatgtcg
     4501 ccaatgacta tgtgctccac aggtatcagt tcttccctgc cattgcgctc tacggtcgcg
     4561 gtgtcgggtc taaggcgtat taatttggat atggcgtcgg tggtgtggct tttagcgcgg
     4621 tcctccaaat aatggccaag ggttatcagg gtcaatatca tcgcggctga ctcgaagtat
     4681 agggtcatcg caaaatcgcg agccttggcg gtatctccga tgaacaggga atcgctcatc
     4741 gcgaacaggg tatacaggcc atacacaacc gccgccgaag agcccaaggc tataagtgaa
     4801 tccatggtgg gagatcgctt ccagagtgtc ttaaagccac gtacaaaaaa aacacggtta
     4861 accactaaaa ccggtaccag taaaagaaac tgaagcaggc cgaaggtaag cggattttgg
     4921 tgaacaaaat ctggaagcgg ccaatgaaac atgtgtccca tggcaatgta aaaaagcggg
     4981 atggtaaaca acagagaggt tgcaaggcgc acggccatgc cctccccctt ttgcagcacc
     5041 gggccatgat ggaaaccagg ggccgaaccg tccgaagatg gaggggcaac taaagtggcc
     5101 gtataccccg cgtcttgaac cgcgccaata atctctgggg ccgtaacaat atcctcatcg
     5161 tgtcgaaccg acatggtttt ggcgatcagg tttacatccg ctttctccac tccggacagg
     5221 gccacaacgc gccgttctac cgttgcagaa caaccagcac aggtcattcc ttctataata
     5281 aacttagtcg taataatcat gtatggagct ccttaacacc caccctgggg tgggggtgta
     5341 cttatataaa agtaagactt tactcatgta cttgtcaagt ttttagccct ttttctcttg
     5401 atttctt
//
LOCUS       DAOQXQ010000021         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16017_length_5253_cov_3.065410, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000021 DAOQXQ010000000
VERSION     DAOQXQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16017_length_5253_cov_3.065410"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKH81_00665"
     CDS             <1..568
                     /locus_tag="PKH81_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNQ96588.1"
                     /translation="LLTFKNPIQLQNAALSALAVKYLFPDLEETVIASGLSKAWLPGR
                     FEIIRNNPLVVLDGAHTVSSITLTRDAFSSVSTKPARLLFACAADKNVERMAEILCPF
                     FTSITLTRPGDRKESDLGHTETAFKQVISTMDDEDTDNRPALEIEENFEKAIRKVWDA
                     SAKEATPLLVTGSFYLVAEMKRILSSQL"
     gene            complement(510..1247)
                     /locus_tag="PKH81_00670"
     CDS             complement(510..1247)
                     /locus_tag="PKH81_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ96589.1"
                     /translation="MKPLPFILSETDDWAVVIKDHGMPTAPLKEGEQGTLLSWFLEHY
                     PSCSAVYGKKNIERGLLHRLDTDTSGCVLIAKNQSAYDYLVQAQDKGQFIKRYRALCN
                     AQPLPGFPRSPVQIPQGSSLNLAITSLFRPWGPGRKAVRPVFTKDCRGECSSKVYTTL
                     LESLEKTHTGTDESFLAQCVLTHGFRHQIRCHLASIGYPISGDRLYNDKPGDGPLCLW
                     AYELTFPDPTTEKKVCVSFQLPDKMSQ"
     gene            complement(1244..2164)
                     /locus_tag="PKH81_00675"
     CDS             complement(1244..2164)
                     /locus_tag="PKH81_00675"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ96590.1"
                     /translation="MNVLDFSAIDPADWGSRLDLVCSRKAGGLSRSRLKTSLYTLCVN
                     GKKVKLSYLVKKGDVIHLEWQDPIPQDIEGEDIPLRIVYEDQNITVVNKVQGMVTHPA
                     AGNWSHTLVNALLWHWKCEVPDGSYRSGIVHRLDKDTSGLIITARNRDTEVYLQEEFK
                     KRRVGKIYIALVCGTPKPLRGEIECSIIRDPKNRKRFTWTDDQTKGKLSKTGYRVVAS
                     FGSYSLVKFKLYTGRTHQIRVHCCKLGTPILGDPIYAKKDNMFPHATLMLHAMTLSIR
                     VPPEGTRHVFRSPIPMRFKRIIQSLTEGTR"
     gene            complement(2161..2739)
                     /locus_tag="PKH81_00680"
     CDS             complement(2161..2739)
                     /locus_tag="PKH81_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96591.1"
                     /translation="MNRIVAILLLLVCLPVFLFSQTEPANSNEPKPYEKDEFSTWQKD
                     VRRAGIITFGALPFSTFFTAIYYDLYRYYDNDQDDRYLPWPLKNNDIAEPLTEDEQKQ
                     ILAISIGASVCVALIDFTVRAIKRSAKRRKAERLERDRVETIRIEEIDGAVPLPTEVP
                     PSSHSDQPEQESESSVNDEFILDEPELEGLSP"
     gene            complement(2736..3431)
                     /locus_tag="PKH81_00685"
     CDS             complement(2736..3431)
                     /locus_tag="PKH81_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNQ96592.1"
                     /translation="MGIKANLERIRDSIHTKEHELALPHGSVNLVAISKFHSQTEVQE
                     AILAGQSIFGENRVQEATEKFIPLIAEYPQITVHLVGNLQSNKISRIVPVASCIQSID
                     RLDLLKPLNRHAQENNKRVEVLFEYHTAEDSKAGFMDDDSVFRAIESCSELEHIIPMG
                     FMTMAPFTDDKNSIRDSFRKTRKLREACLQRFPQYSFPVLSMGMSGDYEIAIEEGSTM
                     VRIGTAIFGERTQ"
     gene            complement(3422..4300)
                     /gene="rsmA"
                     /locus_tag="PKH81_00690"
     CDS             complement(3422..4300)
                     /gene="rsmA"
                     /locus_tag="PKH81_00690"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNQ96593.1"
                     /translation="MSFLPDYDSPLALKAFLDDNGMAMQKKFGQNFLVNRKSRQRLVD
                     ALSIGEGSKVWEIGPGLGAMTEEILSRGAQLTVFEIDRGFARLLKESMGENPRFSIIE
                     GDVLKTWKNTGEAQGFPDRLFGNLPYNIAAEIIASTIQEGIRFERAVVTVQKEVAVRM
                     CAKPDSRDYSSFSVLCQWAYTVSPLMDLAGGNFWPRPNVDSRAVVMKKNELWPGCLNP
                     SLFMKLQRALFSSRRKTVLNNLKPWLISSRPPASLEVDPVEILHQAGIDPGARAETLA
                     IQDFLRLSDVVEESWA"
     gene            complement(4278..>5253)
                     /locus_tag="PKH81_00695"
     CDS             complement(4278..>5253)
                     /locus_tag="PKH81_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNQ96594.1"
                     /translation="AFIIRQGMQSKPRKDGVFSRFRAKLRYHLIRYLGFMGPEGGLFL
                     ALVSGSREYLDPALAQDFRNAGLSHILALSGMHLSLFASLALFICRRFCGKRISPWIA
                     LVVMASFFWFAGNSPSLIRAMLMASLVLAAKKAGYASSLLSILCLAGVIQLCIEPSHI
                     TSLAFLLSYSALAGILALSPTIITLIRPYVPTPISAPLSASVSAQLATMPLCAFLIGS
                     ISPLAIPASIIVSPIASLFLVSSLVLVPFGALVPPCNSLTRGILTLLYTGTQQSVSFF
                     ARVPVLTVQDLFGASLVSILSLSVIIALHALAQHWRLRRSCDVLFTRL"
BASE COUNT         1518 a         1152 c         1192 g         1391 t
ORIGIN      
        1 cctccttaca tttaagaatc caattcaact gcaaaacgcg gccctttccg cacttgcggt
       61 gaaatatctg tttcctgatc ttgaagaaac ggttattgct tcaggccttt caaaggcctg
      121 gcttcccggt cgttttgaga taatccgtaa caatcctttg gttgttctcg atggagctca
      181 cactgtatca agtattactc ttacccggga tgcgttttcc tctgtatcaa caaaaccagc
      241 tcgacttctt tttgcttgtg ctgcagataa aaacgtagaa cgcatggcgg aaattctttg
      301 cccatttttt acgtcaatta cgctaactcg tcctggcgac cgaaaagaaa gcgatcttgg
      361 acatacagag actgctttta aacaggtgat atcaacaatg gacgatgagg atactgacaa
      421 taggcctgct ctagaaatcg aagaaaactt tgaaaaggct atcagaaagg tttgggatgc
      481 aagcgccaaa gaggctaccc cgcttttagt tactggctca ttttatctgg tagctgaaat
      541 gaaacgcata ctttcttctc agttgtagga tcagggaagg taagctcata cgcccataaa
      601 caaagagggc cgtctccggg tttgtcatta tacaaacgat ccccacttat aggatatcca
      661 atactcgcca gatggcagcg aatttgatgt cgaaagccat gggtaagtac acactgggca
      721 agaaacgatt catcagtacc tgtatgtgtt ttctccaggg actcgagtag cgtggtatag
      781 actttcgatg agcactctcc ccgacaatcc ttggtaaaaa cagggcgaac tgcttttctt
      841 cccggacccc agggtctgaa caagctggta attgccagat tcaaagagct cccctgtgga
      901 atctggacag gagaccgggg gaaaccaggt agtggttgtg cgttacacaa ggctcggtat
      961 cgtttgataa attgaccctt atcttgggct tgaacaagat aatcgtaggc actttgattt
     1021 tttgcaataa gaacacatcc agaagtatcc gtatccaaac gatggagtaa ccccctttct
     1081 atgttctttt tcccgtatac agcagagcag gacggatagt gttccagaaa ccaggataaa
     1141 agagtcccct gctctccttc tttaagaggc gcggttggca ttccatggtc ttttataacc
     1201 acggcccaat catccgtttc cgacagtata aaaggaagcg gtttcaccga gtaccttccg
     1261 taagggattg gataattcgc ttaaaacgca tgggaattgg tgagcgaaat acatggcgag
     1321 taccttcggg tggtacacgg atagaaagag tcattgcatg aagcattaag gtagcatgtg
     1381 gaaacatgtt gtctttcttt gcatagatgg gatcaccaag aattggtgta cccaatttac
     1441 agcaatgcac ccgaatctgg tgagtcctgc cggtatatag cttaaacttg accagagaat
     1501 aggagccaaa gctagcaacg actcggtatc cggttttgga taactttccc tttgtctgat
     1561 catctgtcca ggtaaatcgt ttgcgatttt ttgggtccct gataatagaa cattcgatct
     1621 ctccacgtag aggcttgggt gttccacaca ccagggcaat atatatcttt cctacccggc
     1681 gttttttaaa ctcttcttgt aaatagacct ctgtatcacg gtttcgagcc gttataataa
     1741 gcccggatgt atccttgtcc aagcgatgaa ctataccaga gcgatatgac ccatcaggta
     1801 cttcgcattt ccagtgccat aaaagggcat taaccagggt gtgagaccaa tttcctgctg
     1861 caggatgagt taccataccc tgcaccttgt tgactactgt tatgttttga tcttcataga
     1921 caatccgtaa aggaatatcc tcaccttcga tatcctgggg aattggatct tgccattcca
     1981 gatgaatgac atcccccttt tttaccagat atgagagctt aactttctta ccgttaacgc
     2041 agagtgtata tagtgatgtt tttaagcggc ttctgcttaa tcctcctgcc ttgcgggagc
     2101 aaacaagatc caggcgactt ccccaatctg cagggtcaat agcagaaaaa tcaagaacat
     2161 tcatggtgac aaaccttcaa gttccggctc atcaaggata aactcatcgt ttactgaaga
     2221 ttcactttct tgctctggtt gatctgaatg tgatgacgga ggaacttcag ttggcaaagg
     2281 cacagctccg tcaatctctt ctatgcgaat tgtttctacc cgatcgcgct ctaagcgctc
     2341 ggcctttctc cgtttagccg atcgttttat cgctcgcaca gtaaaatcaa taagggctac
     2401 acagactgac gcgccaattg agattgcaag tatttgtttt tgttcgtctt cggtaagcgg
     2461 ttcagctatg tcattatttt ttaggggcca gggtaaatat ctgtcgtcct gatcgttatc
     2521 gtaatatcta tacaaatcgt aataaattgc ggtaaaaaaa gttgaaaatg gcagagcacc
     2581 aaaggttatg atgccggctc ttcgtacatc tttttgccag gtgctgaact cgtctttttc
     2641 atatggtttt ggttcatttg aattggccgg ttcagtttgt gaaaagagaa agaccgggag
     2701 gcagacaagt aaaagaagta tggcaacgat cctgttcatt gagtacgctc tccaaaaatt
     2761 gccgtaccga tgcgaaccat ggttgaacct tcttcaatag ctatttcata atcgccactc
     2821 atccccatgg agagcacagg aaatgaatac tgagggaaac gctgtagaca agcttcccgc
     2881 agtttccgtg tcttacgaaa agaatccctg atagaatttt tgtcatcggt aaatggagcc
     2941 atagtcataa agcccatggg aatgatgtgt tcgagctcac tacaagattc aatggcgcga
     3001 aatactgaat catcgtccat aaaaccagct tttgaatcct ccgcggtatg atattcaaaa
     3061 agaacctcaa ctcttttgtt attctcctgg gcatgccggt tgagcggttt tagaaggtcc
     3121 aggcgatcta tggattgtat acatgacgcg actggtacaa ttcgggatat tttattactt
     3181 tgcaagtttc caaccaaatg aacggttatc tgcggatact ccgcaattag cggaatgaac
     3241 ttttcagttg cttcctgaac tctgttttca ccaaagattg actgccctgc gaggatagct
     3301 tcttgaacct ctgtttgtga gtgaaacttt gatatggcta caagattcac cgatccatga
     3361 ggaagggcaa gttcatgttc tttggtatga attgagtcgc gaatgcgctc gagattagct
     3421 tttatgccca tgattcctcc accacatcac tgaggcgtaa aaaatcctga atagccaatg
     3481 tttcagcgcg tgctccggga tctatacccg cttggtgtaa gatttccacc ggatcaactt
     3541 caagtgaagc aggaggtcgt gatgatatga gccatggttt aagattattg agtactgttt
     3601 ttcttctaga agaaaaaagc gctcgttgaa gtttcatgaa taaggatgga tttaaacagc
     3661 caggccataa ttcgtttttt ttcataacta cagctcgtga atcaacatta ggtcttggcc
     3721 agaaattacc gcctgcaaga tccatcaggg gcgaaacggt gtacgcccat tggcaaagca
     3781 cagaaaagga agagtaatcg cggctgtccg gtttggcgca cattcgcacg gccacttctt
     3841 tttgaacggt gactacggct ctttcaaacc ggataccttc ctgaatagtt gaggcaataa
     3901 tttcagcagc aatattatac ggtaagttgc caaaaagacg atcagggaat ccctgtgcct
     3961 caccagtgtt tttccaagtt tttaatacat cgccttcaat aattgaaaaa cggggattct
     4021 ctcccattga ttctttcaag agtctggcaa aacctcgatc aatctcgaaa actgttagtt
     4081 gagctcctct cgaaagaatt tcttccgtca tagctcctaa tcctgggcca atttcccaaa
     4141 cctttgaacc ttccccgata ctgagggcat caacaaggcg ttgacggctt ttacgattaa
     4201 ccaaaaaatt ctgaccgaat tttttttgca tggccatacc gttgtcatcc agaaaagcct
     4261 ttagcgcaag aggggaatca tagtcgggta aaaaggacat cgcaggacct ccgtaaacgc
     4321 caatgctgcg caagggcatg taatgcaata ataacagaaa gtgatagtat actgacgagc
     4381 gaggcgccaa acaagtcctg aactgtcaga acaggaacac gggcaaaaaa ggaaacactc
     4441 tgttgagttc ctgtatataa caaggtgagt atcccccgag taagtgaatt acagggagga
     4501 acaagcgccc caaaaggcac caaaacaagc gatgatacaa gaaaaagaga tgcaatcggg
     4561 ctaactataa ttgaagcggg tattgccaaa ggggaaattg atccaatcag aaaggcgcac
     4621 aatggcattg ttgccaactg agcagacaca gaggcgctga gaggggctga gataggggtc
     4681 ggaacatagg ggcgaataag ggtgataata gttggagata atgcaagaat accggcaaga
     4741 gcactgtatg agagtaaaaa ggccaatgat gtgatatgag atggctctat acaaagctga
     4801 attacaccgg caagacaaag aatcgaaaga agtgatgaag cgtatccggc ttttttggct
     4861 gcaagaacaa gagatgccat caacatagct ctaatcaaag acggggaatt accggcaaac
     4921 caaaaaaagc tggccattac cactagtgcg atccatggag aaatccgttt tccacaaaat
     4981 cggcgacaga taaaaagtgc cagggaagca aaaagggaca ggtgcatccc tgatagggct
     5041 aaaatatgag aaaggcctgc gttgcgaaaa tcttgtgcaa gggcaggatc aagatactcc
     5101 cgggaacctg acacaagagc taaaaaaagg cctccttctg gacccataaa gccgaggtat
     5161 cgaatgagat gataacgtaa cttcgcacga aaacgtgaaa aaactccatc ttttctcggt
     5221 ttagattgca tgccttgccg gataatgaag gca
//
LOCUS       DAOQXQ010000022         5252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16026_length_5252_cov_3.400231, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000022 DAOQXQ010000000
VERSION     DAOQXQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16026_length_5252_cov_3.400231"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKH81_00700"
     CDS             complement(<1..1297)
                     /locus_tag="PKH81_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700320.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNQ96595.1"
                     /translation="MESTVYRLFVERKSGFDNEAKRALAELTGFLGITGLQAVRYLNR
                     YDIQGVSRQTLETAARQIFSESQSDLVSFESFDCANDETVIAIEYLPGQYDQRADSAC
                     QCLALLSGSEERAIVRCARLFVLKGSLTTQDLERVRRYLINPVDSREADSAQSLSLIM
                     KSERPEPVAILSGFTSLSSQALEAFRTQKGLAMDGADIRFLQQYFTKEGRDPTETEIR
                     VLDTYWSDHCRHTTFNTVLEDIAIPEGPEGQPIRDALAVYSGLRLELYGDRAQGRDGS
                     PAEKPVTLMDMAVIGAKALKKRGLLDDIEESAEINACSVFIDVQMLDTAGNNTGCEKW
                     LLMFKNETHNHPTEIEPFGGAATCIGGAIRDPLSGRAWVYQALRVTGAADPRSPIEKT
                     LPGKLPQIKLTREAAAGFSAYGNQIGLATGQVIEYYDPGF"
     gene            1450..1935
                     /locus_tag="PKH81_00705"
     CDS             1450..1935
                     /locus_tag="PKH81_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNQ96596.1"
                     /translation="MQEILDLLQNDARLTAEDIAAMTKKTVAEVQAIIAELEREGVIL
                     KYSAIINQEKNPAEKDSVRAVIEIQVTPEREHGFDALAERIYRFPQVKSLYLMSGGYD
                     LQVIIEGRTLKEVAFFVSEKLATLNGVKSTKTHFVLKTYKENDIVYVDQKKDRREGVT
                     A"
     gene            1932..3107
                     /locus_tag="PKH81_00710"
     CDS             1932..3107
                     /locus_tag="PKH81_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ96597.1"
                     /translation="MNERTDRYSVKILNTPPSGIRKFFDLIIGRDDIISLGVGEPDFP
                     TPWAMREEAWYHLEQGHTSYTSNWGLTELREAIAGYLTKFDLHYDSKKEILVTIGVSE
                     AIDAVLRATLNPGDEVIVAEPCYVSYQPLVELCDTKLVRLDTSADGFVATAEAIERLI
                     TPKTKALMLCSPSNPTGRMIPAEELAKIAEVVKKHKLWVLSDEVYCELVYDGNTHVSI
                     GSFPGMKDYTIVLNGFSKAFAMTGWRIGYLACPAELMAQVHKLHQYSTICAPIMSQYA
                     ALEGLRSGAAEVERMRVSYQQRRNLMVNAFASMGIPVAEPEGAFYIFPDIRKTGLSSE
                     EFATRLINEYKVAVVPGNVFGAGAEGFVRCCYATEIGKLKEAMRRFALMVEGKPLEQ"
     gene            complement(3114..4031)
                     /locus_tag="PKH81_00715"
     CDS             complement(3114..4031)
                     /locus_tag="PKH81_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96598.1"
                     /translation="MISVTAPKKNLPALITLYVAGILCLCVSCSSAPKRPPAIYASRN
                     NAIAQIDLANRSSSRGEFVSAHLFLDEAWRLAVSTDDPDTRIRVRLSRGNAWYGAGEI
                     IKAEDEWKEALVEAEEAGNAELSGASRVFLARASLAEGRSDVAAEERAERARNARSVA
                     KAELKTLKDNILFTALAWRVIGLCEKELSHPDEAEKAIHQSLALHDKHSYLEDAAWDW
                     FLIASVRSKAGELPLAHTALDESIAFNRRAENSSGLGEAWMAKGLVFEKEGKGDRAAV
                     AYTRARDIFRSALLDAQAEEAERKIAHNQ"
     gene            complement(4050..4997)
                     /locus_tag="PKH81_00720"
     CDS             complement(4050..4997)
                     /locus_tag="PKH81_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNQ96599.1"
                     /translation="MKFKIRYADQIVGFLSLGAVLALVFALVILGSKQRWFARDYNFL
                     TYFETSSGLSVGMPLQYKGFTIGKIKSISLMADDNVEVRFFVYDTYYERAKEGSVIEL
                     IVNPIGLGNQFLLHPGNGKSIAKEGSIIPRLDSAEGIARVNAGFVTIPKRDDTIANLM
                     AQINPLLTNLNTTLSQVNGAFDGSGTGPLANSMTSLERILADVNVITGNLETLSEGLS
                     DANGLIPRLIDSDGKIFDSIEASLSSVEGTLSNLEHLSTLLKGQTPDIARLIQDLHIA
                     VVKGQDVLEGLKNNPLLKGGIPERVQTDSTGTTTRAIEF"
     gene            complement(4994..>5252)
                     /locus_tag="PKH81_00725"
     CDS             complement(4994..>5252)
                     /locus_tag="PKH81_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ96600.1"
                     /translation="PSLLYLDEWTESLDDTSARRMVRLVKKRQEAGHTIVFVSHNLAV
                     IKELAQRICMIVDGRLALDISGEELANNDNLSRMIEKGIAE"
BASE COUNT         1234 a         1308 c         1396 g         1314 t
ORIGIN      
        1 aaaatcccgg atcgtaatat tcaattacct gcccggtggc aaggcctatt tgatttccgt
       61 aggcgctaaa accagctgcc gcctcgcggg taagtttgat ttgcggaagt tttcccggca
      121 gggttttttc tatgggactt ctggggtctg cggctccggt tacccgcagc gcctgatata
      181 cccaggcacg acccgaaagg ggatcgcgga tggcgccgcc aatacaggtt gcagctcctc
      241 caaagggttc aatctcggta ggatggttgt gggtttcgtt tttaaacatc aacagccatt
      301 tttcgcatcc agtgttgttt ccggctgtat cgagcatctg tacatcaata aacacggagc
      361 atgcgttaat ctccgccgac tcttcaatgt cgtcaaggag tccccgcttt ttaagggcct
      421 tggcgccaat caccgccatg tccatcagcg ttaccggttt ctctgcggga gaaccatccc
      481 gtccctgtgc gcgatcccca tataactcaa gacgtaagcc tgaatatacg gcaagggcat
      541 cgcgtatggg ctgtccctcc gggccttcgg gaatagctat gtcttcaaga acggtgttaa
      601 aggtggtgtg gcggcagtga tcggaccaat aggtatccag gacccgtatt tctgtttcgg
      661 taggatctcg cccttccttg gtaaagtact gttgaaggaa gcgtatatcc gctccatcca
      721 tcgccagccc cttttgcgtt ctaaatgcct caagggcctg ggaggatagg gaagtaaaac
      781 cggaaaggat cgcgacaggt tctggtcgtt cgctcttcat gataagagaa aggctttgtg
      841 cgctatcggc ttcgcgggaa tctacggggt ttatcaggta gcgacggaca cgctcaaggt
      901 cctgggtagt aagagagcct ttaaggacaa aaaggcgagc acaacggaca atggcccttt
      961 cttcggagcc actgaggagg gcaaggcact ggcatgccga atcggctcgt tggtcatatt
     1021 ggccgggaag gtattcaatg gcaataaccg tctcgtcgtt ggcacagtcg aaactttcaa
     1081 agctcaccag gtcgctttgg ctttcgctaa agatttgccg tgcggctgtt tcaagggtct
     1141 gtcgggaaac accctggatg tcgtaccggt taagatagcg gacagcctgt aagccggtaa
     1201 taccgagaaa gccggtaagt tccgcaagcg ctcttttcgc ttcgttgtca aagccgcttt
     1261 tgcgctcaac aaacagccgg tatacagtcg attccattac aagtcctccg gtaattttgt
     1321 gggcctatca ttgcatagaa caaaggaaaa ttctatattc cctgcacagg gcaggttgaa
     1381 taaaaataca agctcgtggt atatttattc actataatcc ctggttcaca gggcgtaaag
     1441 gagcactcta tgcaggaaat tcttgacctt ttacagaacg atgcgcgcct caccgcagag
     1501 gacattgcgg ccatgaccaa aaaaacggtt gcggaggttc aggcgataat cgcagagctg
     1561 gaaagggaag gggttatcct caagtactct gcaattatca atcaggaaaa aaaccccgcc
     1621 gaaaaagaca gcgttcgtgc ggtaatagaa attcaggtaa cccccgagcg ggagcatggc
     1681 tttgatgccc ttgccgagcg tatttatcgc ttcccccagg ttaagagcct gtatcttatg
     1741 tccggcgggt atgatcttca ggtcataata gaaggtcgaa cccttaaaga ggttgccttc
     1801 tttgtgtcgg aaaaactggc tacccttaac ggcgtaaaaa gcaccaaaac ccattttgtc
     1861 ctaaaaacct acaaggaaaa tgacattgtc tatgtggatc aaaagaagga ccggcgggaa
     1921 ggggtaactg catgaatgaa cgcacagacc gatattcggt taaaatcctt aataccccac
     1981 cgtcgggaat cagaaagttc tttgatttga ttatcggacg ggacgatatt atctcgcttg
     2041 gcgtgggaga gccggatttt ccgaccccct gggctatgcg ggaagaggcc tggtatcacc
     2101 ttgagcaggg acatacttcc tatacttcca actggggatt aaccgagctt cgcgaggcta
     2161 tcgcgggcta cctgaccaag tttgatcttc attacgactc caaaaaagaa atattggtaa
     2221 ccatcggtgt ttccgaggct atcgatgccg tgcttcgtgc gaccttgaac ccgggagacg
     2281 aggtaattgt tgccgagcca tgttatgtat cctatcagcc cctggtggaa ctgtgcgata
     2341 ccaaactggt ccggcttgat acctctgccg acggctttgt tgctactgcc gaagcgatcg
     2401 agcgattgat tacacctaaa accaaggccc tgatgctctg tagtccaagc aacccgacag
     2461 gccgaatgat tccggctgag gagctggcca agattgccga ggtggtcaag aaacacaagt
     2521 tgtgggtcct gtccgacgag gtatattgcg agctggtgta cgacgggaat acccacgttt
     2581 caattggatc cttcccgggg atgaaggact acaccattgt gctcaatgga ttttccaagg
     2641 cctttgccat gactggctgg cgcattggct accttgcctg tcccgcagag cttatggctc
     2701 aggttcataa gctccatcag tattcaacaa tttgcgcgcc tattatgagt cagtacgcgg
     2761 cccttgaagg attgcgaagc ggggctgccg aggttgagcg aatgagggtg tcatatcaac
     2821 agcgaagaaa cctgatggta aacgcctttg catcgatggg tattccggtg gctgaaccgg
     2881 aaggcgcctt ttacatcttt cccgatattc gcaagaccgg cttaagctca gaggaatttg
     2941 cgactcggtt gattaacgag tataaggtgg cggttgttcc tggaaatgtc tttggagcag
     3001 gtgcggaagg ctttgttcgc tgctgttacg ctaccgagat aggcaagctc aaggaagcga
     3061 tgcgccgttt tgccctgatg gtagaaggga aacctctgga acaataacaa gcgttattga
     3121 ttgtgtgcta tttttcgttc ggcttcttcg gcctgtgcgt cgaggagggc cgatcgaaag
     3181 atatcccgcg cgcgggtgta cgccaccgcc gctctatccc ccttgccttc tttctcaaac
     3241 acaagaccct ttgccatcca tgcctcccca agtccgcttg agttttcggc ccgtcggtta
     3301 aaggcgatcg attcatccag agccgtgtgc gcgagcggga gctctccggc tttagagcgc
     3361 acagaggcga ttaaaaacca gtcccacgcc gcgtcctcca gatagctgtg tttgtcgtga
     3421 agggcaaggg attggtgaat cgctttttcc gcttcgtctg gatgagataa ctccttctcg
     3481 caaagaccga taacgcgcca ggcaagggca gtaaaaagga tgttgtcctt tagcgtttta
     3541 agttctgcct ttgcaacgga tcgggcgtta cgggctcgtt ctgcccgctc ttcggccgct
     3601 acatctgacc ttccttcggc aagagaagcc cgtgccagaa agacgcggct tgccccagag
     3661 agctccgcgt tgccagcctc ttctgcttct acaagcgctt ccttccattc atcttctgcc
     3721 ttgattatct ctcccgcgcc ataccaggcg ttgccacggg aaaggcgaac gcgaatgcgg
     3781 gtgtcaggat catctgtgga aacggcaagt cgccatgcct catccaaaaa aagatgggca
     3841 gaaacaaact ctccgcggga tgaagagcga ttggcaaggt cgatttgggc tatagcattg
     3901 ttgcggcttg cgtatatagc ggggggtcgt ttgggggcgg aggagcagga gacacacaga
     3961 cacaagatgc cggctacata gagagtaatc agtgcgggaa gattcttttt gggagcggtt
     4021 accgatatca tgatgcccat cctttattat tagaattcta tcgcccgggt agttgtgccg
     4081 gttgaatctg tttgaactcg ctcggggatg cctcccttaa gcaggggatt attctttaag
     4141 ccttccagta catcctgtcc tttaaccacg gcaatatgaa ggtcctgtat aagccgtgca
     4201 atgtccgggg tttgaccctt aagcagggtg gacaggtgtt ccaggttgga aagcgtgccc
     4261 tcaacagaag aaagagaggc ttcgatgctg tcaaatattt ttccgtcact gtctatcagg
     4321 cggggaataa ggccgttcgc gtccgaaagt ccctcgctca gggtttcaag attcccggtt
     4381 atcacgttta cgtccgcgag tattctttcc aggcttgtca tgctgtttgc aagcggcccg
     4441 gttcccgatc cgtcaaaggc tccattaacc tgagagaggg ttgtgttcag gttggtgagt
     4501 aaggggttaa tttgcgccat caagttcgcg attgtgtcgt ctcgtttggg tatggttaca
     4561 aaacctgcgt taacccgggc gattccctcg gcggagtcaa ggcgggggat gatgctgccc
     4621 tcttttgcta tgctttttcc gttgccggga tggagtaaaa actgattacc caggccaatt
     4681 ggattgacaa tgagctctat taccgagcct tctttggctc gctcataata ggtgtcgtac
     4741 acaaaaaagc ggacttcaac gttgtcgtct gccatcagcg agatgctttt aatcttgcct
     4801 atggtaaatc ccttgtattg caagggcatt ccaacagaaa gcccggaaga ggtttcaaag
     4861 taggtaagaa aattatagtc acgggcgaac catcgttgct tgctgccaag tataacaagc
     4921 gcaaacacca gggctaacac cgcgcccaaa gaaagaaacc caactatctg atccgcgtac
     4981 cgaattttga acttcattcc gctattccct tttctatcat tctagacaga ttatcgttat
     5041 tggccaattc ctctccggaa atatcgagcg cgagccgccc gtctacgatc atacaaatgc
     5101 gttgcgccag ctcttttata accgcaagat tgtgacttac aaagacgatg gtatgacccg
     5161 cttcttgccg tttttttacc agacggacca tgcgtcgagc ggaggtgtcg tccagggatt
     5221 ccgtccactc atcaaggtac aggaggctcg gg
//
LOCUS       DAOQXQ010000023         5185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16290_length_5185_cov_2.772515, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000023 DAOQXQ010000000
VERSION     DAOQXQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16290_length_5185_cov_2.772515"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1546
                     /locus_tag="PKH81_00730"
     CDS             191..1546
                     /locus_tag="PKH81_00730"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNQ96601.1"
                     /translation="MENHAVSMEKIVSLCKRRGFVFQSSEIYGGQNGAWDYGPLGIAL
                     KNNIQKAWWKEMTQLHDNIVGLDAAILMHPRVWEASGHVANFSDPLVDCKDCKSRFRA
                     DDDTQISPENVEKRTCPKCGGPLTEPRSFNLMFKTSIGPAEDSSSVIYLRPETAQGIY
                     VNYKNIAQSNRLKIPFGIAQVGKAFRNEIVTKNFIFRTCEFEQMEMQFFVKPGEDENF
                     FEYWREQRMSYYPKYGIRKDKLRWHHHDKLAHYAKDAYDIEFEFPMGFQELEGIHNRT
                     DFDLTQHTEYSGKDLQYIDQDNGNERFIPYIIETSAGLTRNVLMFLCDAYEEEKVADK
                     GNDDDWRTVLHFHPSIAPVTVAVLPLMKKDGLAELAKEIQGELKEEFMTDYDQSGAIG
                     KRYRRQDEIGTPFCITIDYDSKEDNTVTLRFRDSMEQVRIARSDLVSRIRAEIKSYSR
                     A"
     gene            1567..2736
                     /locus_tag="PKH81_00735"
     CDS             1567..2736
                     /locus_tag="PKH81_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNQ96602.1"
                     /translation="MAESNKLHSINTSRILRAIWLNPGVSRIKVAEMLDLDRSTVTKI
                     MQVIFDRGLAQTDGKNTSQSGVGRRQVNLKINEDIGVVLGLEIQNSRYRAVMTTISGQ
                     VLYSFDGLQETTGENLVSLIVSLVKKAKKFAKTRGFDILGIGIGLPGILDPYSGIIIR
                     SNPLKIAEPFDVLADLQRYIPEAIVMDNDANSCCWGELAFSPENRNRNFIAVLGEFRN
                     LELADAGYHGLAIGLGLVIREKVLHGDHFTAGEFRAVTANRQTGQFTISWDELKRLPE
                     DSAVLDRVYRELTANLGFLVNSVDFTKIVFAGDLTIHQGNLRSYMADAIEENWTYNLE
                     RSFIIEFSRYGENAVCMGAAGLFVEKLFSVPDIADRFQEVVGYDLFDRILTGRNR"
     gene            2740..3750
                     /locus_tag="PKH81_00740"
     CDS             2740..3750
                     /locus_tag="PKH81_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ96603.1"
                     /translation="MEYVALGKTGLMVSRTAFGALPIQRIESREEAASLVLSAWEAGI
                     NFFDTARSYSDSEAKLSLAFSEIRSDVLIATKSATRTGVELQKDLETSLLELQTDYID
                     LYQLHNPSFLPVPDGADGIYNALLAAKRSGLIRSFGITNHSIAIAREAVTSALYDCLQ
                     YPFSLLSDEQDIQLMNDCVASGMGFIAMKALAGGLLTHIPAAFSWIRQFEEVVPVWGI
                     QRKVELDELLSLEANPPVFDKDMQDVVESERAQLSGSFCRGCGYCLPCPVGIPINNAN
                     RMKQLLRRSPVSQWLTDEWQELMSRIEDCTHCGACAQRCPYGLKPYETLPDHLRDYKT
                     FL"
     gene            complement(3747..5036)
                     /locus_tag="PKH81_00745"
     CDS             complement(3747..5036)
                     /locus_tag="PKH81_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ96604.1"
                     /translation="MNSHYFEKEKETMNRKDLESLQLERLQATVKRVYEKNSVYRKSF
                     ELAGVTPQDIKSLSDIQKLPTINKTIFRDTYPMGLMCVDKKEIVEMHMSSGSTGTPVV
                     MPYTQGDLDQWASCMARCYAMAGALPTDVVQITPSFGLFNGGFGMYHGARKYGLFVVP
                     TGAGNTARQIKLAKDFGTRVFTGVVSYGLRLIEELEAEKTSLSDLRIGIFGAESFSAA
                     MKEKISQGLGIEVFDIYGMTETGGVGTLGQDCPAHNGIHVWEDHYIIEILDRDTHTPV
                     ADGEIGELTITALTREALPVIRFRTGDLSRIISRDTCSCGRTHLRVDQIMGRVDDMLI
                     VKGVNFFPSQVEQALMSIPGVLNNYQIIIEEVNGVTDLRVHVEAENGVTGFMVEKVLK
                     ETLGFSPKGDIFAPGALPRQEGKAKRVFYRKSDGSLA"
BASE COUNT         1293 a         1189 c         1267 g         1436 t
ORIGIN      
        1 atgcacagcc caggagtgta acaatcaggg agggtatcat tagggtattc atataatcag
       61 cataaaggtc aaaaatacag aggtcaatcc ctatttgttt cttatgtaca ttaaccgtgc
      121 aagtatggta ttgttactac caatacagat gtccctcgta accagcgggg gccaaaggag
      181 tatccacaag atggaaaatc atgcagtttc aatggaaaaa attgtcagcc tgtgcaagcg
      241 gcgcgggttt gtctttcagt cttccgagat ttatggcgga caaaatgggg cttgggatta
      301 tggcccgctt ggaatcgctc taaagaataa catccaaaaa gcttggtgga aggaaatgac
      361 ccagcttcac gacaatatcg taggacttga tgccgcgatt ttgatgcatc cccgcgtttg
      421 ggaagcatcc ggacacgtcg ccaatttttc cgatccgctg gtagactgta aggattgtaa
      481 aagtcgcttc cgtgccgatg atgataccca aatctcgcct gagaatgttg agaaacgaac
      541 ctgccccaaa tgcggcggcc ctcttaccga accccgttcc tttaatctta tgtttaaaac
      601 ctcgattggt cccgccgagg actcttcgtc ggttatctac cttcgtccgg aaaccgcaca
      661 gggtatctat gtaaactata agaatattgc acagtcaaat cgccttaaga ttccctttgg
      721 tatcgcccag gtaggcaagg cgtttcgtaa cgagattgtt accaagaact ttatcttccg
      781 aacatgcgag tttgagcaga tggaaatgca gtttttcgta aaacccggcg aggacgagaa
      841 cttttttgaa tactggaggg agcagcgcat gagctattat cccaaatatg gtattcgtaa
      901 ggataagctt cgctggcacc accacgataa actcgctcat tacgccaagg atgcctacga
      961 cattgaattt gaattcccca tgggatttca ggaacttgaa ggtattcaca accgtactga
     1021 ttttgacttg acccagcaca ccgaatattc aggcaaggat ttacagtaca ttgaccagga
     1081 taatggcaat gaacgtttta ttccctatat tatcgagacc agcgcaggtc ttaccaggaa
     1141 tgtcttgatg ttcctctgcg acgcctatga agaggaaaag gttgccgaca agggtaacga
     1201 cgacgattgg cgaacggttc ttcattttca cccatcaatc gctccggtta ccgttgcggt
     1261 gcttccgttg atgaagaaag atggccttgc agaacttgcc aaggaaattc agggtgagct
     1321 taaagaagag tttatgaccg attatgacca atccggtgcg attggtaagc gctatcgccg
     1381 acaagatgaa atcggtacac ccttttgcat tactatcgac tacgattcca aagaagataa
     1441 cacggttacc ctcaggttcc gcgactctat ggaacaggtt cgcatcgcgc gaagtgattt
     1501 agtttcccgc attcgcgcgg agattaagag ctatagccgg gcctagggtt cagggatacg
     1561 cgttgtatgg cagagagtaa taaactccat tcaatcaata cctcgcgtat cctcagggct
     1621 atctggctta atccaggggt tagccggatt aaggtagccg agatgcttga tcttgaccgt
     1681 tctacggtaa ccaagattat gcaggttatc tttgaccgcg gtcttgccca gacagacggt
     1741 aagaatacaa gccagtccgg ggttgggcgc agacaggtta accttaagat taacgaggat
     1801 atcggggtcg ttcttggtct tgaaattcaa aactcccgat accgagccgt gatgactact
     1861 atatccggtc aggtgctgta ttcctttgac gggttacaag aaacaaccgg ggaaaaccta
     1921 gtttccctta ttgtttccct ggtaaaaaaa gctaaaaagt tcgccaagac ccgaggtttt
     1981 gatatattag gtattggcat cggtttaccc ggtatccttg acccctattc gggaatcatt
     2041 attagatcta atccgctcaa aattgctgaa ccttttgatg tgcttgcgga tttacaacgc
     2101 tacattcccg aggcgattgt tatggataat gacgcgaact cctgttgttg gggagagctc
     2161 gcctttagtc ccgagaatcg aaaccgaaat tttatagcag tattagggga gtttcgtaat
     2221 cttgaactcg cagacgcggg atatcatggt cttgcgatag gtcttggctt ggttattcgg
     2281 gaaaaagtgc ttcatgggga tcattttacc gccggtgaat ttcgtgctgt taccgcgaat
     2341 cggcaaaccg gtcaattcac catctcctgg gatgagttga aacggcttcc cgaggatagt
     2401 gcggtattag accgtgtcta tcgggagctt actgcgaatc ttggtttctt ggttaactca
     2461 gtcgatttta ccaagattgt gtttgctgga gatttgacga ttcaccaggg aaaccttcgc
     2521 tcatatatgg cagacgcgat tgaagagaac tggacatata accttgagcg atcttttatt
     2581 atcgagttct cacgatatgg ggagaatgca gtgtgtatgg gtgctgcggg attattcgtg
     2641 gaaaaacttt tttcggttcc cgacatcgcc gacaggtttc aggaagtggt tggctatgat
     2701 cttttcgatc gcattcttac cggcaggaac cgttaaccag tggagtatgt agccctcgga
     2761 aaaacaggac tgatggtttc ccgcactgcc tttggggcac ttcccattca gcgtatagaa
     2821 agtcgggaag aggctgcttc gctggtgctc tccgcctggg aagcagggat taattttttt
     2881 gatacagctc gctcatattc tgatagcgaa gcaaaacttt cgcttgcctt tagcgagatt
     2941 cgctcggatg tattgattgc aactaaatct gctacgagaa cgggagtgga attacagaaa
     3001 gatcttgaga cgagtcttct tgaactgcaa accgattata tcgatctcta tcagcttcat
     3061 aatccttctt tcttaccagt gcctgacgga gccgatggta tatacaatgc tcttctggca
     3121 gcgaaacggt ccggacttat ccgaagcttt ggtataacaa atcattcaat tgctattgcc
     3181 agggaagcgg taacatccgc tctctatgat tgtctgcaat atcccttttc cctgttatcc
     3241 gatgaacaag acattcaatt gatgaatgac tgtgttgctt cggggatggg atttattgcc
     3301 atgaaggcac tcgccggtgg cttgcttact cacatccccg cagccttttc atggattcgt
     3361 cagtttgaag aggtagttcc ggtgtggggt attcagagaa aggtcgagct tgatgaattg
     3421 ctttctttag aagccaatcc acctgttttt gataaagaca tgcaagatgt tgtggagagc
     3481 gagcgtgccc aactatccgg gagcttttgc cggggatgtg gctattgctt gccctgtcct
     3541 gtcgggattc ctataaataa cgcgaatcgt atgaagcaac tcctgcgtcg ctcaccggtc
     3601 tcccaatggc ttaccgatga gtggcaggaa ttgatgagcc ggattgaaga ttgcacacac
     3661 tgcggtgcat gcgcccaacg atgtccttac ggccttaagc cgtatgaaac cttacccgat
     3721 catctgcgcg attacaaaac ctttctttaa gccagacttc catcgctttt gcggtagaat
     3781 acccgctttg cctttccttc ctggcggggg agggctcctg gggcaaatat gtctcccttg
     3841 ggagaaaagc cgagcgtttc tttaagtact ttttctacca tgaagcccgt gactccgttt
     3901 tctgcctcta catgcactcg caggtcggtt accccgttta cttcttctat aatgatctgg
     3961 tagttattca atacgccagg aatcgacata agcgcctgtt caacctggct ggggaagaag
     4021 tttacaccct taacgataag catatcgtct acgcggccca ttatttggtc aacgcgcaga
     4081 tgagtgcgtc cgcaagagca ggtgtcgcgc gaaataatac gggaaaggtc tccggttctg
     4141 aaccggatta ccggaagtgc ttctcgggta agagcggtaa tggtaagctc tccgatttcg
     4201 ccatccgcta ctggagtgtg cgtgtcccga tcgagtattt cgataatata atggtcttcc
     4261 cacacatgaa taccgttatg agcggggcaa tcctgtccaa gagtgccaac tcctccggtt
     4321 tcagtcattc cgtagatatc gaatacttct atgccaaggc cctgggaaat tttttctttc
     4381 atggcagcag agaaggattc agcgccaaag ataccgatac gtaaatcgct caagcttgtt
     4441 ttttcggcct ctaattcctc gatgagcctg agaccgtacg acactacacc ggtgaatacc
     4501 cgggttccaa agtctttggc gagttttatt tgacgggcgg tatttccggc cccggtggga
     4561 acgacgaata atccgtattt cctcgctccg tgatacatcc cgaagccgcc gttaaaaaga
     4621 ccaaaagagg gggttatttg cactacatcg gtaggaagcg cgcccgccat ggcatagcat
     4681 ctggccatac agcttgccca ttgatcaaga tctccttgcg tatagggcat gacaactggc
     4741 gtgccggtag aaccggagga catgtgcatc tctactattt cttttttatc cacgcacata
     4801 agtcccatgg ggtaggtgtc acgaaaaatg gttttgttga ttgtaggaag tttttggata
     4861 tcagaaaggc ttttgatatc ctggggagta actccggcta gttcaaagct ttttcggtag
     4921 accgaatttt tttcgtatac tcgcttaacc gtggcttgaa gacgttcaag ttgaagcgac
     4981 tcaaggtcct ttcggttcat tgtctctttt tctttttcaa aataatggct attcattttt
     5041 taaaagctcc tccggcggca aaggcctcag tgtttagttg acgatgtttt tccgggatac
     5101 aagtttctat cgctcgattc catccatctg caagacaggg taactctttt gcggccgcgc
     5161 ccagggtaac gatattggca gcctt
//
LOCUS       DAOQXQ010000024         5146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16435_length_5146_cov_3.316441, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000024 DAOQXQ010000000
VERSION     DAOQXQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_5146_cov_3.316441"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKH81_00750"
     CDS             complement(<1..302)
                     /locus_tag="PKH81_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96605.1"
                     /translation="MKLSPLARRVNRLSVLSFFIIQISTVIVSEVFSQMFNPTKNLDY
                     MGRLHLAANPKLAVFIILICSATGFLICAYLKPLWIELETAEERRTPKNSARARMVA"
     gene            complement(385..969)
                     /locus_tag="PKH81_00755"
     CDS             complement(385..969)
                     /locus_tag="PKH81_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96606.1"
                     /translation="MQNSPAKNFSRFLTDRFSSEWKLLTETERYLSNTPDAPLYESRF
                     RLWREKLNRISRGSTELISLRSEIVDLRKELRLKGYDLSLGLQTLVLEGFRDDTAMAE
                     GFSRVVLCFTNVGLFFHTGSANHIALAQEMDIRLHTLKGITSRESHYLWYARTAQGLI
                     LSGGATETKESFLRLEALVTANPLKLLSALKDLR"
     gene            997..1764
                     /locus_tag="PKH81_00760"
     CDS             997..1764
                     /locus_tag="PKH81_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ96607.1"
                     /translation="MKILLVSDIHANLDALTAVLDETLQSVDGLVCLGDITGYGPDDE
                     ACVQRLRDVSLDLEESLVLAGNHDAALGGGLALTWFNTEAQESIKRSASLLSMESMEW
                     LSSLLPQGYAEFGAERVYAVHGSPVDPLTGYILDKASANLSFNRMEASGLRRCFFGHT
                     HECALWKPGVNIGAIAGYPDIGKSFVADSYLFNPGSVGWPRYIMRPEYGADGIGQDSL
                     WPAWYALWETDTGKIQVCTARYDPSSMLARLADRDKR"
     gene            complement(1772..3730)
                     /locus_tag="PKH81_00765"
     CDS             complement(1772..3730)
                     /locus_tag="PKH81_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ96608.1"
                     /translation="MFRKTLNTCYTPSMNLLSVSRLARSAREGLLFSQVTFGIDEGEK
                     VALIGHNGSGKSTLLACIAGLLDPDQGQSVINKEAGCSFLPQNPVFRPEDSILDHVFS
                     SNSPSLSVIRDYRALCDRLATQENLSATLSDTLARVTHRMDEGNLWNWEYRIESILTS
                     LGITDLSRKMGELSGGMLKKVSLAQVLVEDTRLLLMDEPTNHLDIETIAWLENYLCET
                     ERSVLMVTHDRYFLDAVCTSIYELDNRVLTRYEGNFSRYLEKKQIEEEIAANTETRIE
                     SVLRTEREWLMRGPQARGTKARARVDAIRKMINRDKREVKTEYAFEVTGRRMGGKVLT
                     AEGLSKSFPVPGGTKTVIKDFSWTFTPGERIGIFGSNGSGKTTLLNLLSGELEPDQGI
                     VSRGETMVFGYYRQNPELSFPDQTVLEYIQDTAELIRLADGRDLTASQLLERFGFTGK
                     VQHSPVATLSGGEKKRVYLVRILMSNPNFLILDEPTNDFDIYTMGVLESFLSGYKGCL
                     LVVSHDRFFMDRVAETLFILDDEGTIRHVPFSTSDYLSYRAAEEAERTQKIAAEKRNR
                     PIENTAISTDSLDTKPKKRSFKEQKEFDQIETRIGEMEEQKGTMEALLSGGESDHQKI
                     RTIAEEYEKLSRELESAYERWEHLASLC"
     gene            3747..4385
                     /gene="nth"
                     /locus_tag="PKH81_00770"
     CDS             3747..4385
                     /gene="nth"
                     /locus_tag="PKH81_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNQ96609.1"
                     /translation="MRMIDARGIEKVFMRLSRANPEPKTELESVNDFTLLVAVVLSAQ
                     MTDKGVNRATGPLFALVDNPQAMVDLGEEGLKTFISSVNLYPTKARHIIALSAMLLER
                     YGGKIPSTRKELEGLPGVGPKTASVILNVVFGQATVPVDTHLWRIAPRIGLSVADSVR
                     AMEEDLISKIPARYIHSAHHWLLLHGRYVCTARKPLCDSCCINDVCPRNGLS"
     gene            complement(4382..>5146)
                     /locus_tag="PKH81_00775"
     CDS             complement(4382..>5146)
                     /locus_tag="PKH81_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNQ96610.1"
                     /translation="LSRNYAPQDDTHIGQGERVFYTSRNARYLDTARALIAQVPLQWQ
                     DLLLAPLLSEASIHANTAGVFKGFYKNAETGIGTFGGKKGDALSRIMGNIHLELPVTS
                     AFDCPAIVLSGDANEVSRIAPEVDIAYLDPPYNQHPYGSNYFMLNLLVDGNEPEKPSA
                     ISGIPAHWNRSRYNKRIEAALALEDLVSSLKASYLLISFNSEGFISRDIMETLLQKFG
                     EVHIMETPYSVFRGSRNLAGREKQVTEYLYLVKKNA"
BASE COUNT         1208 a         1178 c         1375 g         1385 t
ORIGIN      
        1 gctaccattc gcgctcgggc agagtttttg ggggtgcggc gctcttccgc ggtttccagt
       61 tcaatccaaa ggggttttag ataggcacag ataagaaagc cggtagcgct acagatcagg
      121 ataataaaca ctgcaagctt gggattggcc gcaaggtgta aacgccccat ataatcaagg
      181 tttttggtgg ggttaaacat ctgtgaaaaa acctcactga caataaccgt ggagatttga
      241 atgataaaga atgatagaac cgaaagacgg tttactctgc gggccagggg agatagtttc
      301 atgagaagcc tcttttttga ttcattgttt ccttattgta ttcctgtttt gctcaagaat
      361 caaatttcac ttggtaaaat atcttcatcg caagtcctta agggctgata atagcttgag
      421 ggggttggcg gtgaccagcg cttccagtct gagaaacgat tcttttgttt ctgtagctcc
      481 tccggagagg ataagcccct gtgcggtacg ggcgtaccac aggtaatggc tctcgcgaga
      541 ggtgatgcct ttgagggtat gaagccgtat gtccatttcc tgggccaggg ctatgtgatt
      601 tgcgctgccg gtatggaaaa agaggcccac attggtaaag cagagcacta cccgggaaaa
      661 tccttctgcc atggcggtgt cgtcacgaaa tccttccaga acgagcgttt gtagccccaa
      721 agaaaggtca tagcctttaa gacgaagctc ttttctcaga tcgacaatct cgcttctaag
      781 gcttatcagc tcggttgaac ccctggaaat gcggtttagc ttctctcgcc agaggcggaa
      841 gcgtgactcg tacaggggtg cgtccggggt gttggataga taccgttctg tttccgtaag
      901 cagtttccat tcggaactga agcggtcggt gaggaaacgg gaaaaattct tggcagggct
      961 gttttgcatc tacaagaaga gtatacttca ggatgtatga aaatactgct ggtttccgat
     1021 atacatgcta acctggacgc ccttaccgcg gttcttgatg aaacgttgca aagcgtagat
     1081 ggactggtgt gtttaggcga tataacgggg tatggccctg atgacgaagc ctgtgtgcag
     1141 agacttcggg atgtttctct tgatttggag gaatctcttg tccttgcagg gaatcatgac
     1201 gcggctcttg gcggaggtct tgcgcttacc tggtttaaca cagaggcaca ggaatcgatt
     1261 aaacgctcgg caagtctttt gtccatggaa tccatggaat ggctatccag tttattaccg
     1321 cagggttatg cagaatttgg cgctgagcgt gtgtatgccg ttcatggctc cccggtcgac
     1381 ccccttacgg ggtatatcct tgataaggca tccgcgaacc tttcttttaa ccggatggaa
     1441 gcaagtggcc tccgtcgatg cttttttggg catacccacg agtgtgccct atggaagccg
     1501 ggagtgaaca tcggagcgat agcggggtat ccggacatag ggaaatcctt tgtcgccgat
     1561 tcatatttgt ttaatcccgg tagcgttggt tggcctcgct acattatgag accggaatat
     1621 ggggccgatg gaataggcca agactcacta tggcctgcct ggtatgccct gtgggaaaca
     1681 gatacgggca agattcaagt gtgtacggcc cgctatgatc cttcctctat gcttgcccgg
     1741 cttgccgacc gcgataagcg gtgattcaag actagcagag agaggcgagg tgttcccatc
     1801 gctcatacgc actttccagc tcccgggaga gcttttcata ctcttcggca atagtgcgaa
     1861 tcttttggtg atcactttcg ccgcctgaga gaagggcttc catcgttccc ttttgctcct
     1921 ccatctcacc aatacgcgtt tctatctgat cgaattcctt ttgctctttg aagctccgtt
     1981 ttttgggttt ggtatctaat gagtcggtag aaattgcggt gttctctatt ggtcggtttc
     2041 gtttttctgc tgcgattttt tgagtgcgtt ctgcttcttc ggctgcccgg taggaaagat
     2101 agtcgcttgt agaaaaggga acgtgacgta tcgttccttc atcgtccaga ataaaaaggg
     2161 tttctgcgac acgatccata aaaaaacgat cgtgcgagac tacaaggagg catcccttgt
     2221 aaccggacag gaaggattca agaacaccca tggtatagat atcaaaatcg tttgttggtt
     2281 cgtccagtat caggaagttg ggattactca ttaagatacg tactaaatag actcgctttt
     2341 tttctccccc cgacagggtg gcaacaggcg agtgttgaac ttttcccgta aaaccaaagc
     2401 gttccagcag ttgtgacgcg gttaagtccc tgccgtctgc aaggcggata agttcggcgg
     2461 tatcctgaat atattccagt accgtttgat ccggaaacga tagctcgggg ttttgtcggt
     2521 aatacccaaa gaccattgtc tcgccgcgtg agacaatgcc ctgatctggc tccagctccc
     2581 cggataacag gtttaggagc gtggttttgc ctgaaccgtt tgatccgaaa attccaatgc
     2641 gctctcccgg ggtaaaggtc caggagaaat cctttataac ggttttggtt cctccaggaa
     2701 cagggaagga tttggaaagg ccttcggccg tcaatacttt tccacccatg cgtctgccgg
     2761 ttacctcaaa ggcgtattct gtcttaactt cccgcttgtc acggttaatc attttgcgga
     2821 tggcatccac ccgcgccctg gccttggttc cacgggcttg tgggcctctc attagccatt
     2881 cacgctctgt gcgtaatacc gattctatgc gggtttcggt attggccgct atttcttctt
     2941 caatttgctt tttctccaga tagcgagaaa agtttccctc gtagcgcgta agaacgcgat
     3001 tgtccaactc ataaatagag gtgcacaccg catcaagaaa gtagcggtcg tgggtaacca
     3061 tgagcacact gcgttctgtt tcgcagaggt agttctcgag ccaggcaatg gtttcaatat
     3121 ctagatggtt agtcggctca tccatcaaca gtagtcgcgt gtcttctacc agcacctgcg
     3181 cgagtgaaac ctttttaagc attccaccag acagttcccc catttttcgg gaaaggtcgg
     3241 taatgccaag gcttgtcagg atggattcga tacgatattc ccaattccac aggttccctt
     3301 catccatgcg atgggtaact cgggcaaggg tgtccgaaag cgttgcggag agattttcct
     3361 gagtggcaag ccgatcgcac agggcacggt aatcgcgaat tacggacaaa gagggactgt
     3421 tggagctgaa tacatggtcc aggattgaat cttccgggcg gaagaccgga ttttgcggaa
     3481 ggaaggagca cccggcttcc ttgtttatta cggattgacc ctggtctgga tccagaaggc
     3541 ctgcgatgca cgccaagagg gtgcttttac cggagccatt gtggccaatc agggcaactt
     3601 tttctccctc atcaatccca aaggttacct gcgaaaacaa taaaccctcc cgtgcggaac
     3661 gggcaaggcg gctaacagac aaaaggttca tggacggagt atagcaggta ttgagcgttt
     3721 tacggaacac gggctacact cggagcatga gaatgattga tgcacgaggg attgaaaagg
     3781 tgtttatgcg tttgtccagg gcgaatccgg aaccaaagac cgagctggaa agcgtgaatg
     3841 attttacact tctggtggcg gtagtccttt cggctcagat gacagataag ggtgttaacc
     3901 gcgctaccgg gcctttattt gcgttagtgg ataatccaca ggcaatggtt gatctgggag
     3961 aagaaggcct taaaacgttt atctcatcag ttaatctcta tccaacaaag gctcgccaca
     4021 ttattgccct aagcgcaatg ctccttgaac ggtatggagg aaagattcct tctacgcgta
     4081 aggagttgga gggtttaccg ggggtaggac caaaaacagc aagcgtcatt ttaaatgtgg
     4141 tgtttgggca agcgactgtc cccgtggaca cacatctgtg gcggattgcc ccccggattg
     4201 gtttaagcgt tgccgactca gttcgcgcta tggaagagga tttgatctcc aagattcccg
     4261 cacggtatat acatagtgcg catcactggc tcttgctcca tggacggtat gtatgcactg
     4321 cccgtaaacc cttgtgcgat agttgttgca ttaatgatgt gtgtccaaga aatggacttt
     4381 cttaagcatt ttttttaacc aggtagaggt actctgtaac ctgcttttcc cttcctgcaa
     4441 gattgcgact tccccgaaaa acactatagg gtgtttccat gatgtgcacc tctccgaatt
     4501 tttgcaggag tgtttccatt atgtctcgcg agataaatcc ttcggagtta aaagagataa
     4561 gcagatagga tgccttgagg gaagagacaa ggtcttcaag cgcgagggcc gcctcgatac
     4621 gtttgttgta ccgggaacga ttccagtgtg cgggaattcc tgaaatcgct gatggctttt
     4681 cgggttcgtt gccatccacc aacaagttaa gcataaaata gttcgatcca tagggatgtt
     4741 ggttgtaggg ggggtcaagg taggcaatat caacctccgg ggcgatacgg cttacctcgt
     4801 ttgcatctcc cgataatacg attgccggac agtcaaaagc gctggttact ggtaattcaa
     4861 ggtgtatgtt acccattatt cgtgaaaggg cgtctccttt ttttcctcca aaggtgccga
     4921 tacctgtttc tgcgttttta taaaaaccct taaagactcc tgcggtgttt gcatgaatgg
     4981 atgcttcgga caagaggggg gcaagcagga ggtcttgcca ttgcaggggg acttgagcga
     5041 tcagtgctcg cgcggtatca aggtatcggg cgtttcgcga ggtataaaac acgcgctctc
     5101 cttgcccgat gtgtgtgtca tcttgtggcg cataattacg ggaaag
//
LOCUS       DAOQXQ010000025         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16444_length_5143_cov_4.488208, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000025 DAOQXQ010000000
VERSION     DAOQXQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16444_length_5143_cov_4.488208"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKH81_00780"
     CDS             <1..987
                     /locus_tag="PKH81_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96611.1"
                     /translation="SAPAITTTPAAAGYTEVLTSGEWSFVKTLPDDIAEGLGWRLHVL
                     AEDASGNRTDPGVAASVVFDVDRSDPVASETTYISAEIVNIKTSFSLEGEAIDSHGIF
                     SLTVSQKQDDGAVVPINTNGPTLGGTVTNRTWTLANLPRNPADIGVATTVNGVYEYII
                     TATDLAGKTHSVTRRLRFDTIGPELTVVSPADAYWSPAATIAFNGVASDLTAVDKVYY
                     QRTAESVVVAPDIPADPGVEANWTGAGVGGGWTLVTGKNTWNFSLTEAGEGRQKVWLA
                     AVDTAANPSYRYPASMVWTLIVQPLTLPYPNRTPVFCLVFQAMQRIQMLWPI"
     gene            879..>5143
                     /locus_tag="PKH81_00785"
     CDS             879..>5143
                     /locus_tag="PKH81_00785"
                     /inference="COORDINATES: protein motif:HMM:NF023666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNQ96612.1"
                     /translation="MDLNSPALNSSIPEQNTRVLFGLSGDAADTNALADISTIVITQS
                     KDGAAAETVPGITVTLAGDEKSATWSVSGLPRIPGSMADSVPLTTASDGEYEYVITAT
                     DVSGKTRQRTASVRFDRTEPVMAISTAIPATWFPLAAFPISGTNSDGGSGIGAVEYSL
                     DGTNFSALSFTTTNWSGTIPVSSGVNTIRLRISDRAGNQTVLPDQTLNVDLEAPSVVV
                     TDPSEILRVRSGMDIPITFDAWDENPGSGLQSVSVTRVGSQTLATPPSVAYTGDMSIT
                     GGTITLPWATLDALAPALVDGTQYVVTVRATDRGERTGSSTFSFVYDATPPSVAIAAP
                     ALSDPATLLSQTINQTVTVSGTASDTQMLRAINSVSLDIVDGSGTVLMNLDTFSGAAA
                     NTWSYNLDTTNATVLSHGTAVGLGTRIILRATAWDAAGNTNFVDRPVTINQESDRPVI
                     TLSNLITDPTDPDYPSILQMIKTVYGSVLDDDGITSVQANNGDGWTVVPLTGSGFTYT
                     TTGTDGSKSLSFRIVDSAGRTFETGTATQPQFVNGTGFVDGPVPYSVDTVYPEIFATI
                     RVDTQTPYDFAAGVSDLTTNKPFGGSTAVFALEVRASDANGIDTVSVTVPGAPGSPYV
                     ATASGLEAGFTIFRTGPIDVSTVPDGAVVVTALVTDKSGLEKSSSNTILVDNTVPTVT
                     NDYPRMGVDQVNGLLDISGQALDSGSGLFSVEYNLGYDYASQPWAPVSGTFSWKISFV
                     GAAPPDVGEVSGTIEQAAGIISVDLNPDSDLFTAPSHGLANGTPVWLGAETLPTGHLA
                     PGVVYYVIDSAADTFRLSASSGGAMVDFTDAGLAIYVSAKSSSPAGDNFWQLPVFIRA
                     TDNAGNVYTTAEWAYRITVDPSGDKPKAYISYPDTTPSILGGIIRINGYAEDDDAVDS
                     VYMQIDVNGDGLFTALDVAGATDWYSGGEGRPVTGGTSWRQSINESQEFNPVEVSAPE
                     ILTGIVYRITELGTTDFTLLGAPAGFVVGTEFTATSDGNAGSGNGRAEPTKRTIHFRV
                     RAKDIYGTFGAWTSAYQIDVDKNVPKFGYTNAFTLTQGTIERLYQPDMWVRGSWVLSG
                     TIEDESGIDRIEISSIVTGSGSSIPVSLTENPRVSFTNGTTGDQGYELSIQIPTAALP
                     AGNTLAVTITAFDNSTPQMSAQQTFSIKYDNIAPTINAYEGVTPVVNTNYLYEAKGSV
                     VETGSGFERVFVYFQRTGTVSGVDRIYNPMLPVPDSRVNTSDLNFVLDSDVPLFAIPT
                     FTKTSTTLQSASLANNDNVRVGSVVYLDGLYCRITDYNSGTGTITWDEDVSQAVTNAA
                     IVYALVLDNTTAEYPVWAPDYSSYTITNDDGDGLIESVTSAGALYEWKTSVDSRNIPD
                     GNITVHYVAFDKAGNKAHGSQATSVRNNPPLLAKVTLATDLSG"
BASE COUNT         1189 a         1313 c         1351 g         1290 t
ORIGIN      
        1 tccgcacctg ctattaccac aacccctgca gcggccgggt ataccgaggt gctcaccagc
       61 ggtgagtggt catttgtcaa gacgcttcct gatgacattg ccgaaggcct gggctggcgc
      121 ctccatgtgt tggccgaaga cgcatccggc aaccgcaccg accctggtgt tgccgcgagt
      181 gtggtctttg atgtagaccg cagcgaccct gtcgcttcgg agacgacgta tatcagtgcg
      241 gaaatcgtca atataaagac gtcctttagt ctggaaggcg aagcaatcga tagtcatgga
      301 atttttagcc tgacggtttc tcaaaaacag gatgacggcg ctgttgttcc cataaatacg
      361 aacgggccaa ctctgggcgg aacggtgacc aatcgcacct ggaccttagc gaatcttccg
      421 cgtaatcccg cggatatagg agtggcaacc actgttaatg gtgtgtatga atacattatt
      481 accgccactg atcttgccgg caagactcat tcggtaacaa ggcggcttcg ctttgatacg
      541 ataggacccg agcttacggt tgtatctccc gcggatgcct attggtcgcc cgcggctacg
      601 attgccttta acggtgtggc ttccgatctt actgccgtag ataaagtgta ttatcagaga
      661 accgccgaat cggtggttgt tgctcccgac atccccgctg atccgggcgt ggaagcaaac
      721 tggactggag ccggcgttgg tggtggatgg acgctggtta ccggcaagaa tacctggaat
      781 ttctccctta ccgaagcagg cgaaggcagg caaaaggtgt ggctcgcggc tgttgatacc
      841 gcggcaaatc cgtcctaccg atatcccgcc tctatggtgt ggaccttaat agtccagccc
      901 ttaactcttc catacccgaa cagaacaccc gtgttctgtt tggtctttca ggcgatgcag
      961 cggatacaaa tgctctggcc gatataagca ctattgtgat tacccaaagc aaggatggag
     1021 ctgcagcaga aaccgttccg ggcataaccg ttacccttgc aggtgacgaa aagagcgcga
     1081 cctggtcggt aagcggttta ccgcgtattc caggcagcat ggctgattcg gttccgctta
     1141 caactgcctc cgatggagaa tatgaatatg taattaccgc cactgatgtg tcgggtaaga
     1201 cacggcagag aacggcgtcg gttcgctttg atcgtaccga accggttatg gcgatttcta
     1261 cggctatacc ggcaacctgg ttcccgcttg cggcattccc catcagtgga acgaattccg
     1321 atggtggtag cggtattggg gctgtggagt actctcttga tggcactaat ttctctgcac
     1381 tctcgtttac cacaacaaac tggagtggta ccatccctgt gtcctctggt gtaaatacca
     1441 tacgcttgcg tatttccgat cgcgccggga accaaacagt tcttcccgat caaaccttga
     1501 atgtcgattt ggaagcgcct tccgttgtgg tcaccgatcc ttcggaaatt cttcgagttc
     1561 gatctggcat ggatattccg ataacctttg acgcatggga cgaaaacccc ggtagtggac
     1621 ttcagagtgt atctgttacc cgggttggct cgcaaaccct tgcgactccc ccttctgttg
     1681 cgtataccgg cgatatgtcg ataaccggcg gcaccattac cctgccctgg gcaactcttg
     1741 acgccttggc tccggctctg gtagacggga cgcagtatgt ggttactgta cgcgctaccg
     1801 atcgtggcga acgcacggga tcttctactt tctcctttgt gtacgacgca actccgcctt
     1861 cggtagctat tgctgccccg gctctaagcg atccggcaac gcttctttcg caaacgatta
     1921 accaaaccgt aacggtaagc ggtaccgcaa gcgacacaca gatgctcagg gccatcaatt
     1981 cggttagcct tgatatcgtt gatggatccg gtacggtttt gatgaacctt gacaccttct
     2041 cgggcgcggc ggccaatacc tggtcgtata accttgatac cacaaacgcc acagtcctct
     2101 cccatggcac cgcggtgggt ctgggaacgc gaattattct acgcgccact gcctgggatg
     2161 cagcgggcaa cacgaacttt gtggatcggc cagtaacaat caatcaggaa tcggaccgac
     2221 cggttattac cctgtccaac ctgatcactg acccgactga tcctgattac ccgtcaattc
     2281 tccagatgat aaagacggta tatggttcag tattggatga cgatggcatt acctctgtac
     2341 aggcgaataa cggcgatgga tggacggtag ttcctcttac tggtagcggt tttacctata
     2401 ccaccacggg taccgatggc tccaagagcc ttagcttccg gattgtggat tccgccggcc
     2461 gtacctttga aacaggtact gccacgcaac cccagttcgt taatggtacg ggctttgtcg
     2521 atggtccagt cccgtatagc gtagacaccg tttatccaga aatttttgct acaatcagag
     2581 tagacaccca gactccttat gattttgccg cgggtgtctc tgatctaaca actaacaagc
     2641 cctttggtgg atctaccgca gtctttgctc ttgaggttcg cgcaagcgat gctaacggca
     2701 tcgacactgt gtcggtaacg gttccgggag caccgggctc tccctatgtc gctacagcct
     2761 ccggactgga agcaggtttt actatcttcc gtactggacc tattgatgtg tctactgttc
     2821 ccgatggtgc ggtggtggta accgctttgg taacggacaa atctggattg gaaaagagtt
     2881 cgtctaatac catcttggtg gataataccg ttccgacggt aacgaacgat tacccgcgaa
     2941 tgggcgtaga tcaggttaat ggacttcttg atataagcgg gcaggctctt gacagcggct
     3001 ctggattatt tagcgttgag tataatcttg gttatgatta tgcaagccaa ccctgggctc
     3061 ctgtttcggg aacatttagc tggaagatct cgtttgtagg agctgctcct ccggacgttg
     3121 gggaagtaag cggtaccatt gaacaggctg caggtattat ctccgttgac ttaaatcctg
     3181 atagcgacct gtttactgcg ccctctcatg gtcttgccaa cggcacgccg gtgtggcttg
     3241 gtgcggaaac actgcccacg gggcatctgg ctccgggagt tgtgtattat gtaatagatt
     3301 cagcggcaga tacattccgg ctttccgcaa gctccggcgg ggcaatggtg gactttaccg
     3361 atgcagggct tgcaatatac gtaagcgcaa aatcgagtag tcctgcaggg gataacttct
     3421 ggcaactacc ggtatttata cgtgcaaccg acaatgcggg taatgtctat acaacagcgg
     3481 aatgggcata ccggattacc gttgatccct caggtgacaa gcccaaggcg tatatctcct
     3541 atcccgacac tactcccagt atccttggag gaattatccg tattaacggt tacgccgaag
     3601 atgatgacgc ggtagacagc gtgtatatgc agatcgatgt taatggtgat ggtcttttta
     3661 ccgctcttga cgtagcgggt gctaccgact ggtattctgg cggcgagggt cgcccggtta
     3721 ccggtggaac gagctggcga cagtcgatta acgaaagtca ggagtttaat ccggttgaag
     3781 tgtcggctcc cgaaattctg acgggtattg tctaccggat taccgagctt gggactacgg
     3841 actttaccct ccttggagct cctgcaggct ttgttgttgg cactgaattt accgcaacct
     3901 ctgatggtaa cgccggaagc ggtaacggaa gggcggagcc aaccaaacgc acaatacact
     3961 tccgcgtacg tgcaaaagat atttatggaa cttttggtgc ctggacttca gcatatcaga
     4021 ttgatgtgga caaaaacgtt cccaaatttg gatacaccaa tgcatttacg cttactcaag
     4081 gaaccataga aagactgtat caacccgata tgtgggttcg cggctcatgg gtgctctctg
     4141 gaactatcga agatgaaagc ggtattgatc gcatagagat cagctccata gtaaccggca
     4201 gcgggtcttc catacccgtc tcgctgacag aaaatccgcg agtctcgttt accaatggca
     4261 caacgggcga tcagggatat gagctttcta ttcaaatacc aacggcagcc ttgccggcgg
     4321 gtaatactct tgcggtaact ataaccgcct ttgacaacag cacgccgcag atgtcggcac
     4381 agcaaacctt tagcatcaag tacgataata ttgccccaac aataaatgcg tatgaggggg
     4441 ttactcctgt tgttaatacg aattatctct acgaggctaa gggctctgtg gtagagaccg
     4501 gtagcggttt cgagcgcgtc tttgtgtact tccaaagaac tggtactgtc tccggcgtgg
     4561 atcgcatata caaccccatg cttccggtac cggatagtag agtcaataca agcgatttga
     4621 actttgtcct tgattctgat gtaccactgt ttgctatacc tacgtttacc aagacaagta
     4681 ccacgcttca atcggcttct ttggctaata acgacaatgt tcgagtcggg tcggtagtgt
     4741 accttgacgg gctgtactgc agaattactg attacaacag tggaacgggt acaataacct
     4801 gggacgagga tgtatcgcag gcggttacaa acgcagctat tgtctatgcc ctggtccttg
     4861 ataataccac tgccgagtat ccagtgtggg cacctgatta ttcgagttat accatcacga
     4921 atgacgacgg tgatggattg attgagtcgg taacgagcgc gggcgccttg tatgaatgga
     4981 agacctctgt ggattcgcga aacatacccg acggtaatat tactgttcac tatgtggcct
     5041 tcgacaaggc tggcaataaa gcccatggct cgcaagcgac atcggtgcgc aataatcctc
     5101 cgctgttggc aaaggttacc ctggccacag atctttcggg taa
//
LOCUS       DAOQXQ010000026         5114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16579_length_5114_cov_2.651710, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000026 DAOQXQ010000000
VERSION     DAOQXQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16579_length_5114_cov_2.651710"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKH81_00790"
     CDS             complement(<1..365)
                     /locus_tag="PKH81_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96613.1"
                     /translation="MTKLSRMFFIKSAFAACLLVCLASCTKPNKVTLSGSLPPVPLTG
                     YQASKTPTDPKALPAVKKELAFLASLSNDKREGVISGQNVGHGDQILNPSGFFGYEPL
                     VAELERQTGEVPGMIGLDF"
     gene            553..807
                     /locus_tag="PKH81_00795"
     CDS             553..807
                     /locus_tag="PKH81_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96614.1"
                     /translation="MRTTLILEDETMITLKELAARQSRTLTEVVSETLNRGLGLTTEK
                     LEPWTCGSFDMGGGFDYTKAWERVNELEASAVAEKMDARK"
     gene            804..1232
                     /locus_tag="PKH81_00800"
     CDS             804..1232
                     /locus_tag="PKH81_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ96615.1"
                     /translation="MIIPDLNVLLYAVDSSSPRNREALSWLESAVNSRKESVGIPWVV
                     HLGFLRLTTNPRVFSRPLTVETASDWLESLESAPSVRMIDPGTAHRGILRHLLLMLGT
                     GANLVTDAHLAALALEHNAVLATGDRDFQRFPGLKLEVLF"
     gene            1287..1508
                     /locus_tag="PKH81_00805"
     CDS             1287..1508
                     /locus_tag="PKH81_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNQ96616.1"
                     /translation="MPYSATRLRQNLYAILDSVLENGQPVEIERKGKILRIVPEERPS
                     IWERLEPHSVVTGDPEDLVSLDWSGVAEP"
     gene            1505..1879
                     /locus_tag="PKH81_00810"
     CDS             1505..1879
                     /locus_tag="PKH81_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ96617.1"
                     /translation="MIFLDTHAVVWLYQKDKDRFSDLALQLLESDDLRISPTVSLELE
                     YLFKIGRLTERSRTILSWLGRSIRLREDDAAFTAVVEASLDASWTRDPFDRLIVAQAS
                     LHDAPLLSKDRTIRDHYRHARW"
     gene            complement(1864..2109)
                     /locus_tag="PKH81_00815"
     CDS             complement(1864..2109)
                     /locus_tag="PKH81_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96618.1"
                     /translation="MYGKREAQLSRGVKHPSGVLIHRPCPLATAARRSGKPADPLALN
                     FPNAFDGLKGIQFVAKVIESSRANGAWVICAEDHQRA"
     gene            2163..2753
                     /locus_tag="PKH81_00820"
     CDS             2163..2753
                     /locus_tag="PKH81_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96619.1"
                     /translation="MIQTHQMVMDTLSTYGSPKAKLTRMVSNGEMIRIRRGLYIDDLS
                     IPRMALAPVIYGPSYISFNTALSWHGLIPERVEIIESASFGKNRDKTFHTPLGEYHYR
                     YLPASAYPHGLRLQEDSGYQFLIASREKALCDAMYKAGVLAHEKDIEALLIENWRIEM
                     EDIESLDKNLVRFLAPLYRKKALTVFASWLEKESHP"
     gene            2750..3631
                     /locus_tag="PKH81_00825"
     CDS             2750..3631
                     /locus_tag="PKH81_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ96620.1"
                     /translation="MTDLSPVKKMLDEYQCKTNEDRSAALREIVQELALVGLSRTDFF
                     SCAAFYGGTALRIFHGLERYSEDLDFSLLTPHASFSLNDYLPAIRDELASWGFEMTVE
                     QKNKTVEGAVQSAFIKGGTLVHLVKIAAVDPPVSGVPPNSLLRVKLEIDTDPPDGARF
                     ETKYRLRPIPFAVRLYDPSSLFAGKVHAVLCRKWKDRVKGRDFFDFLWYLSNNIPLNL
                     AHLEQRMRQSGHWEDVPLLEKNLRQLLQERFATVNYAKAKADAIPFVRDSRMVDLWSA
                     DFFIAVTKDRLKIANQV"
     gene            3646..4824
                     /locus_tag="PKH81_00830"
     CDS             3646..4824
                     /locus_tag="PKH81_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96621.1"
                     /translation="MQSKKIFASFFLAVVTLVVSSVEAEEGIHAEYVRTHSGDGKYLS
                     VSPDGSKFAVVLGKVITIIDSATGEVLADSSEANTAFDPRSFCWSPDSSLAAFTEDYY
                     RLMRDSDVWILTIKDNALIRVFADDIQKPFRAEEGNSATNPVWSPDGKLLFVSCVDYA
                     SRSSVIRSITNLDESTAEVVFSETPENGPGIWSSMFPVGDSLYFCRDTPKKNDEGDGI
                     FRVHLSNGHIETVLSPDETRGEPLLLAVSADGTYALAIHFLYAMSFTSTFFNAGTGVY
                     ESGVFLIDLSDYSKRPLEIWKSGDEVSRIITASFSPDGSQVVYLSQEGKDGHILMIQD
                     TKGGGPRVLFSNDEDNTLLFGDSAVSYKPQLFWTRDNRLFALGNKGDALHEFTLVTGE
                     "
BASE COUNT         1206 a         1311 c         1307 g         1290 t
ORIGIN      
        1 tcgaaatcaa ggccgatcat tcccggcact tcgcccgttt gtcgctccag ttcggcgaca
       61 aggggttcgt acccgaaaaa gccggaggga ttgaggatct ggtcgccgtg accgacgttt
      121 tgtcccgaga tgactccttc tcttttatca ttggagaggg aagcaagaaa ggccagttct
      181 ttttttaccg ccggcagggc tttggggtcg gtgggtgttt tcgaggcttg gtagcccgtc
      241 agcggtaccg ggggaagcga accggagagc gtgactttat taggttttgt gcaggatgca
      301 aggcatacta gaaggcaggc ggcgaacgcg ctttttatga aaaacatcct ggataattta
      361 gtcatatgaa tgctccttat tcatcttttc ttattaaagg gaagtatagc atggaatggc
      421 ggggaatcag agttcgcgcc tcgtggcgct cccttcgcgg cggctccgcg cgtagtagtt
      481 cgcgtctcgt tacgcccgct gcctcaattt atatgttttg acacatcctc tatggcgtag
      541 taatatgtat atatgagaac aaccctcatt ctggaagacg agacgatgat caccctcaag
      601 gagttggcgg cgcggcaatc gcgaacgctc acggaggtcg tgtcggaaac gctgaaccgg
      661 ggactgggac tgacaacgga aaaattggaa ccctggactt gcgggagttt cgatatgggc
      721 ggcggatttg actatacgaa ggcatgggag cgggtcaacg agcttgaagc gtccgcggtg
      781 gccgaaaaaa tggacgcgcg aaaatgatca tccccgattt gaacgtgctc ctgtacgcgg
      841 tggattcttc ctctccccgg aatcgcgagg ctttatcctg gctggaatcg gccgtcaatt
      901 cacggaagga atccgtcggc atcccctggg tcgtacacct cgggtttctc cgccttacca
      961 caaacccgcg agtattcagt cggcccctga cggtcgaaac ggcaagcgat tggcttgagt
     1021 cgctggaatc cgcaccgtct gtccggatga ttgatcccgg cacggcacat cgcggaattc
     1081 tccggcacct cctgctcatg ctcggaacgg gtgccaacct tgtaaccgac gcgcacctcg
     1141 ccgcgctcgc cctcgagcac aacgccgttc tcgccacggg cgaccgggac tttcagcgat
     1201 tccccgggct caaactggaa gtgttgtttt agcgtacgtg ttgtatttta tgtacgtacg
     1261 cattatactg gatacaggag gataccatgc cctatagcgc gactcggctg agacaaaatc
     1321 tctatgccat actggacagc gttctcgaga acggccaacc cgtcgaaatc gagcggaagg
     1381 gaaaaatcct ccgcatcgtg cccgaagaac gcccgtcaat ctgggaacgc ctggaaccgc
     1441 atagcgttgt taccggcgac ccggaggatc tggtttccct tgactggtcg ggagttgccg
     1501 aaccgtgatt ttccttgaca ctcatgccgt cgtctggctc tatcaaaaag ataaagaccg
     1561 tttctccgac ctcgcgctgc aattgctgga aagcgatgac ctacgcattt cacccacggt
     1621 gtcccttgaa cttgagtatc tgtttaaaat cggacgcctg accgagcgaa gcagaacaat
     1681 tctttcctgg ctcggccggt caatccgcct cagggaagat gacgccgcat tcacggcagt
     1741 agttgaggcc agtctcgacg catcctggac acgagacccg tttgaccgcc tgattgtggc
     1801 acaggcaagt cttcacgatg cgccactcct ctccaaagac cgcaccatca gggaccatta
     1861 ccgtcacgcc cgctggtgat cctccgcgca gataacccag gcgccgttag cgcgggagga
     1921 ttcgatgacc ttcgccacga actggatgcc cttgaggccg tcgaaggcgt tcgggaaatt
     1981 gagggcgagc gggtcggcgg gttttccgga ccggcgggcg gctgtcgcga gcggacaagg
     2041 gcgatggatc aacaccccgg aggggtgttt aactccccgc gacagttgtg cttctctttt
     2101 accgtacatt acgcttgttt atcaccagaa agtacagtat attgatactt actggaggta
     2161 aaatgataca aacccaccag atggttatgg atacgctctc tacctatggc tctcccaagg
     2221 cgaaattgac acgcatggtt tcaaacgggg agatgatccg gatacgacgc gggttgtaca
     2281 ttgatgatct gtcaatcccg cgcatggccc tggcacccgt gatctatggc ccctcgtata
     2341 tatcctttaa taccgctctt tcctggcacg gtctgatacc agagcgcgtg gaaattatcg
     2401 aatcagcaag ctttggaaaa aatcgtgaca agacatttca cacacctctt ggtgagtatc
     2461 actaccgata cctccccgct tctgcctatc cacatggact gcgtctacaa gaagatagcg
     2521 gataccagtt ccttatcgcc agtcgtgaaa aggccttgtg cgacgccatg tacaaggcag
     2581 gagttcttgc tcacgagaaa gatatcgaag ctctgctgat agaaaactgg agaatcgaaa
     2641 tggaagacat tgagtctctg gacaaaaacc ttgtccgctt cctggcaccc ctttacagga
     2701 aaaaagcgtt aaccgtattt gcttcctggc ttgaaaagga gtctcatcca tgacagacct
     2761 ctcccccgta aaaaagatgc ttgatgaata ccagtgcaag accaacgagg atcgaagcgc
     2821 tgccctccgt gaaatcgtac aggaactagc ccttgttggc ttatcgcgga cagacttttt
     2881 ctcgtgcgcg gccttttacg gaggaacggc cctccggatt tttcacggtc ttgagcggta
     2941 ctcggaagat cttgatttta gtcttcttac cccccatgcg tcctttagcc tgaatgatta
     3001 cctgccggcg attcgtgatg agcttgcttc ctggggattt gaaatgacgg tggaacaaaa
     3061 aaataaaaca gtagagggtg ctgttcagtc agcctttata aagggaggaa ctctggtgca
     3121 tctggtaaag attgctgctg tagacccacc cgtgtcaggt gttcctccca acagcctgtt
     3181 acgggtaaaa ctggaaattg ataccgaccc gcctgacggt gcacgttttg aaacaaaata
     3241 ccgtttacgt cccatcccct tcgccgttcg actctatgat ccttcctcac tttttgcggg
     3301 aaaagttcac gcagttctgt gccggaaatg gaaggaccgg gttaaaggtc gcgacttttt
     3361 tgattttctg tggtacttat ctaataacat tcccctaaac ctggcacacc ttgaacagcg
     3421 catgcggcag tcgggacatt gggaggatgt tcccttgctg gaaaagaacc tgagacaatt
     3481 attgcaagag cgtttcgcga cagtgaatta tgctaaagcc aaggccgatg ccattccctt
     3541 tgtacgcgat tcccgcatgg ttgatctttg gtccgctgat ttttttatcg ccgttaccaa
     3601 agatcgctta aaaatagcaa atcaagttta ataaaaggaa aaaccatgca gtctaaaaaa
     3661 atctttgcat ctttctttct ggcggtagta accctggtag tttcttctgt agaagccgaa
     3721 gaagggatac atgcggaata tgttagaacc cacagtggag acgggaaata tctatcagtc
     3781 tctccggatg ggtccaagtt cgcagttgtt ttggggaagg taattacgat tatcgattct
     3841 gctacaggcg aagtccttgc cgacagctcc gaagccaaca ccgcctttga tccgcgttcc
     3901 ttttgttggt ccccggattc ttccctggcc gcctttaccg aggattatta ccgactgatg
     3961 cgggacagtg atgtgtggat tcttactata aaagacaatg ctcttatccg tgtatttgct
     4021 gacgatattc aaaaaccatt tagagcggaa gaagggaatt cggcaacaaa tcccgtttgg
     4081 agcccagacg gaaaacttct gtttgtttcg tgcgtcgact atgcctcgcg ctcttcggta
     4141 atcagaagca tcaccaatct ggatgagtca acggcggagg tggttttctc cgaaacccct
     4201 gaaaacggtc ccggcatttg gagcagtatg tttcctgtgg gagattctct ttatttttgc
     4261 cgcgacactc ctaaaaaaaa tgacgaaggt gacggaatct tccgcgttca tctttcaaac
     4321 ggccacatcg agacggttct ttctcccgat gaaacaaggg gagagccgct acttttggct
     4381 gtgagcgctg acggtaccta cgcactcgcg attcactttt tatacgccat gtcttttacg
     4441 tcaacttttt ttaatgcggg aaccggggta tatgaatcgg gcgtatttct gatcgacttg
     4501 tctgattatt caaagaggcc tttggaaatc tggaaaagtg gagacgaggt atcgcgtatt
     4561 ataaccgctt ctttttcccc cgatggaagc caggtggtgt acctgtccca agaaggaaag
     4621 gacggtcaca tactcatgat tcaggacacc aagggcggag gcccccgcgt cctcttttcc
     4681 aacgacgagg acaatactct attgttcggc gattctgccg tttcgtacaa accgcagctc
     4741 ttttggacac gcgacaacag gctttttgca ctgggaaaca aaggggacgc actccacgag
     4801 ttcacgctgg ttacaggtga gtaaaaaaaa cgcggacttt gaaagtccgc gtgctatctt
     4861 ttttaaggcc gccttaccct aaacgaacta tacgcatgtc acttaatttc aaacgttatt
     4921 tctttatttg caagttcact cggcttaaga tacttatagg tgacgctctt tgcagtgcac
     4981 gcaacgggca ccatgatgat ttgagcgttt tcttcatata ctgttctctc ctttattatt
     5041 ttaatcttcg taaaagacgt acggtttact ctgctcttct tgacgggcgc gggcttcgct
     5101 catatcgacg cagc
//
LOCUS       DAOQXQ010000027         5049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_16865_length_5049_cov_3.375050, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000027 DAOQXQ010000000
VERSION     DAOQXQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16865_length_5049_cov_3.375050"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="hisIE"
                     /locus_tag="PKH81_00835"
     CDS             <1..990
                     /gene="hisIE"
                     /locus_tag="PKH81_00835"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNQ96622.1"
                     /translation="IGSAAFTLSDGSPGVNAEFLDALAQAVGKERIIVSVDARDGKIA
                     VKGWTESAGIDLVEGAQAVEKWASELLFTCVEREGTMTGIDLDLVTKLRQAVSCRLVV
                     AGGVSTVDEVVALERLGCDVQLGMALYTGKVSLADGFISCLDWEKEALLPVVAQAESG
                     EVLMVGYANREALAKTFESGNLTFWSRSRSKLWTKGETSGNTLSVVRLRVDCDRDTIL
                     ATVRPAGPTCHTGAWTCFQTTADRPYSLPFLSSIIADRFANPRPGSYTATLTAERVRE
                     KIEEEAEELIEAEGKDDVVWEVADLLYFMNVLMYREGVSWQDVLDELDKRHKK"
     gene            1006..2340
                     /gene="hisD"
                     /locus_tag="PKH81_00840"
     CDS             1006..2340
                     /gene="hisD"
                     /locus_tag="PKH81_00840"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNQ96623.1"
                     /translation="MSVIRTVTSREIPDSFYSIRRLEDAVEVVRGILSSVRDRGDEAV
                     RECASRFDRANPASFEVDPAMLADAEQKMKKDKPALYDALCLSRDLALSFAKRQRESF
                     DDFESELSPGLITGQRTIAVDRAGLYVPGGRFPLFSSVIMCASPARAAGVGELVFCTP
                     PVAQSDPALADIPCVDEAIMATASLCGITRVFAIGGAQAIGAMAYGTHSVPKVNVIVG
                     PGNKYVAAAKKMVYGEVGIDLLAGPSEVFIIADGSADFRWVARDMLAQAEHDPDAQAV
                     LVTNDSLLAERVEKELETLLEPLGSDSPAALSFSANGWIILCESLDEAASIANRKAPE
                     HLELALEAGAVRDALETTLRNYGSLFIGHKSAEVLGDYAAGLNHTLPTSSSALFTGGL
                     SVRHFLKTVTTLRTAERPGSDVRGLNASLAAAALLADAEGLCGHALAARVRL"
     gene            complement(2361..3245)
                     /locus_tag="PKH81_00845"
     CDS             complement(2361..3245)
                     /locus_tag="PKH81_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ96624.1"
                     /translation="MHPDSLFSRYQPLFPELKRLSRAQNTITEENVYQEANKGIRAIM
                     DRMCAETMRPDSQFRNIDHAEDFLARIKSGAKGIILMEHYSNYDLPGVMYLLEKSGPS
                     GKELASRIIAIAGMKLNEDNPMVSAFAEAYSRIIIYPSRSLAAISDPEIFKREEQRSR
                     VINLASMRTLDRVRKEGNAVLVFPAGTRFRPGKPETKRGVREIDSYIRLSDIMMLISI
                     NGNCLRFSDTPSDMLSDVVCEDRIIMTASPVYDCAAFREEARSWGGDNCEDKKQLVVD
                     YVMHRLEVMHEENETDRL"
     gene            complement(3300..3947)
                     /locus_tag="PKH81_00850"
     CDS             complement(3300..3947)
                     /locus_tag="PKH81_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNQ96625.1"
                     /translation="MLRHLLLDLDNTLYPASDAMDKGITKRMMDFVASYLNLPLEDAI
                     ALRKEGLPAYGTTLEWLKACHGLTDEGAYFTAVHPENEIEELTPDPSLRPYLLSLGLP
                     LTLLTNAPMAHAKRVLDFFNIGDIFIGVFDLTFHKGRGKPHPESFLDTLARVGFSVEE
                     TLFVDDHPKYVRGYKALGGKAVLVDEQGRYQSLETEEGFCRIHSIYDLAGILCNY"
     gene            complement(3960..>5049)
                     /locus_tag="PKH81_00855"
     CDS             complement(3960..>5049)
                     /locus_tag="PKH81_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96626.1"
                     /translation="VFRTASEQTIRANKRQDGLYHAYNTLSIATSGGMDVQYLNEMLE
                     GQVAVISSLLLSSKEVLDLCKAMKKGPLFRKDQYSYILYPNKELPHFCAKNIVNEEDA
                     LRIPSLARLLASGNTSIMFQDSQRACHFNPSFRNAQVMEAAFNTLSEAIPQADRQSIL
                     ELYEKTFNHQSFTGRSGTFYAYEGLGSIYWHMVSKLMLAVQEYALAEQDETVAQSLTD
                     LYYDIRSGIGFNKTPEIYGAFPTDPYSHTPAGQGAKQPGMTGQVKEEVITRWGELGVS
                     VSNGIVSFAPRMLRTQEFHSQGTLSFSWCAVPVEYRLVPKTERPRISCILGGKEHSRE
                     GNALDSTESKALLGRTGEIERIIVTVPL"
BASE COUNT         1138 a         1189 c         1405 g         1317 t
ORIGIN      
        1 attggttccg ctgcgtttac tctttctgac ggtagtcccg gcgtaaacgc ggaattcctt
       61 gatgctcttg cccaggctgt aggtaaagag cgtattattg tgtcggtaga cgcacgcgat
      121 ggaaagatag cggttaaggg atggacggag agtgcgggta ttgatttggt agagggcgct
      181 caagcagttg aaaaatgggc gagcgagctg ttgtttacct gtgtagagcg cgaaggtaca
      241 atgaccggca tagaccttga tctagttacc aagcttcgtc aggcggtatc gtgccggctt
      301 gttgtagccg gtggtgtttc taccgttgat gaggttgttg cccttgagcg actgggctgc
      361 gatgttcagc ttggtatggc cttgtatact ggcaaggttt ctcttgccga cggatttatc
      421 tcctgccttg attgggaaaa agaggcattg cttcctgtgg ttgcacaggc ggaaagtggc
      481 gaagtgctta tggttggcta tgccaatcgt gaggccttgg ctaaaacatt tgaaagcgga
      541 aatcttacct tttggagccg ctcccgttca aagctttgga caaagggcga aacatcaggg
      601 aacacccttt cggtggtacg tttgcgggtc gactgcgacc gcgacaccat tcttgccaca
      661 gttcggcctg ccggacccac gtgtcacacg ggggcatgga cgtgctttca aaccacggcg
      721 gatcggccgt atagccttcc ctttctttcg agtattatcg cagatcgatt tgctaatcct
      781 cggcccggct cgtacaccgc aaccctgact gccgaaagag ttcgggaaaa gattgaagag
      841 gaagctgaag agctgatcga ggcagaaggt aaggacgatg tggtatggga agtagcggac
      901 cttttgtatt ttatgaatgt gttaatgtac cgcgagggtg ttagctggca agatgtgctg
      961 gatgaactcg acaagcgaca caagaagtag agaggaggat tccctatgtc tgtaatccgc
     1021 accgtaacga gccgggaaat tccggattct ttttattcta ttcgccggct tgaagacgct
     1081 gtagaggtag tacgcggaat cctttcgtcg gtaagggata gaggcgatga ggcggtacgc
     1141 gaatgtgctt ctcgttttga tcgggcaaat cctgcttcct ttgaagttga tcctgccatg
     1201 cttgccgacg ccgaacaaaa aatgaaaaaa gacaagccgg ccctgtacga cgccctctgt
     1261 ctttcccgcg atcttgccct ttcttttgcc aaacgtcagc gagaatcttt tgacgatttt
     1321 gaaagcgaac tttctcccgg ccttattacc ggtcagcgaa ccattgctgt agatcgtgcg
     1381 ggtctgtatg taccaggcgg acggttcccg cttttttcga gtgtgattat gtgcgcttcg
     1441 cctgctcgtg ctgcaggcgt tggcgaactt gtcttttgta ctcctccagt tgcacaaagt
     1501 gatccggctc ttgcggatat tccctgtgtg gatgaagcaa taatggctac tgcctcgttg
     1561 tgcggaatta cccgcgtgtt cgccattggt ggcgcacagg ctattggcgc gatggcctat
     1621 ggtactcaca gcgtacccaa ggttaatgtg attgtaggcc cgggcaataa atacgttgca
     1681 gcggcaaaaa aaatggtata tggagaggtt ggcattgatt tgctcgcagg cccttcagag
     1741 gtatttatta tcgctgacgg gagcgctgat tttcgatggg tggcccgaga tatgcttgcg
     1801 caagcggaac atgatcctga cgcgcaggca gttctggtaa caaacgacag ccttcttgcc
     1861 gaacgtgtcg agaaagaact tgagaccctt cttgaaccac taggttctga ttctcctgct
     1921 gcgctcagct tttccgcaaa cggatggatt attctatgcg agagtcttga tgaagcggct
     1981 tctattgcca atcgcaaagc cccagaacat cttgagcttg cccttgaagc tggagcggtt
     2041 cgggatgccc ttgaaacgac acttcgaaac tatggctctc tttttatcgg gcataaaagc
     2101 gccgaggtgt tgggtgatta tgcagcagga cttaaccata ccttgcccac ctcatcaagc
     2161 gcgcttttta ccgggggact gtcagtgcgc cacttcctta agacggtaac aactctgcgt
     2221 accgccgagc gtccgggctc tgatgttcgg gggctgaacg catctctggc ggcagctgcc
     2281 ttgcttgcgg atgccgaagg gctttgcggg catgctcttg ctgcccgagt acgcttgtaa
     2341 ctggcgttta ggcttttgta ctagaggcga tctgtttcgt tttcttcgtg catgacttcg
     2401 agccgatgca taacatagtc gactacaagt tgctttttat cttcgcagtt atcgccaccc
     2461 caggaccgag cttcttcccg gaacgcagca cagtcgtata ccgggcttgc ggtcatgata
     2521 atgcggtctt cgcatacaac gtcgctgagc atgtcggatg gtgtatccga gaaacgcaga
     2581 cagttaccgt tgatggatat taacatcatt atatccgaca ggcgaatgta ggagtctatc
     2641 tcgcgaaccc cacgtttggt ttctggtttg cccgggcgga agcgggttcc tgcgggaaac
     2701 acaagaaccg cgttgccttc ttttctgacc ctgtccagtg tccgcattga agcgaggttt
     2761 attactcggc ttcgctgttc ttcccgctta aagatttccg gatcggaaat agccgctaaa
     2821 gatctgctgg gatagatgat gattctggaa tatgcttcgg caaaggcaga aaccatgggg
     2881 ttgtcttcgt taagcttcat gcctgctatc gcgataatac ggcttgcgag ttcctttccc
     2941 gaaggtcctg atttttccaa aaggtacatc acccccggaa ggtcatagtt gctgtaatgt
     3001 tccataagga tgatgccctt ggcaccagat ttgatgcggg caagaaagtc ttccgcatgg
     3061 tcaatattgc ggaattgtga gtcaggtcgc atagtttcgg cacacatgcg gtccatgatg
     3121 gccctaatgc ctttattggc ttcctgatag acgttttctt cggttatcgt gttttgcgcc
     3181 cgagagaggc gcttcagttc cggaaaaagc ggctggtaac gactgaatag tgaatcggga
     3241 tgcatgtcgt ctccttgaat aaacttggca ttcagtaata ccggttagct cgattactgt
     3301 caataattgc agagaatccc ggcaagatcg tagattgaat gaatacggca gaagccttct
     3361 tctgtctcaa ggctttggta gcgcccttgt tcgtcaacca gaacggcctt gccccctagc
     3421 gccttatagc cgcgcacata tttgggatga tcgtccacaa agagggtttc ttccacgcta
     3481 aacccaaccc ttgcaagggt gtccaaaaag gattctggat gcggcttccc cctgccctta
     3541 tgaaaggtaa ggtcaaaaac gccaataaag atatccccga tattaaaaaa atcaagaacc
     3601 cgcttagcgt gagccatggg agcattggtg agaagggtaa gcggaaggcc cagggatagc
     3661 agataggggc gcagagacgg atctggagtg agctcctcta tctcgttttc cggatgaacc
     3721 gcggtaaaat atgcgccctc gtcggtaagc ccgtggcaag ccttaagcca ttcaagggtt
     3781 gtgccataag cgggaagtcc ttcttttctg agcgcgattg catcttccag cggaagattc
     3841 agataggaag cgacgaagtc catcatccgc ttcgtaatgc ccttgtccat ggcatcagaa
     3901 gcggggtaca gggtgttgtc aagatcaagg agaagatgtc gaagcatagc gtgagtgtat
     3961 cataaaggga ccgtcacgat aatacgctcg atttctcccg tacgaccaag aagcgccttg
     4021 gattcagtgc tgtccagggc atttccctcg cgggagtgtt ccttgccgcc caggatacag
     4081 ctgatgcgtg gacgttctgt ttttggaacc aaccggtatt caacgggaac ggcgcaccag
     4141 gagaaagaaa gggttccctg tgagtgaaac tcttgtgtac gaagcatgcg gggcgcaaag
     4201 gaaacaatgc cgttggatac cgataccccg agttcacccc aacgggtgat tacttcttcc
     4261 ttgacctgtc cggtcatgcc gggctgttta gccccctgtc ctgctggagt atgcgagtag
     4321 ggatctgtgg gaaaggctcc gtagatttcc ggtgttttat taaagccaat acccgagcgg
     4381 atgtcgtagt aaagatcggt aaggctttga gcaacagttt cgtcttgttc tgcaagggca
     4441 tattcttgta ccgcgagcat caatttggaa accatgtgcc agtagatgct accaagaccc
     4501 tcataggcgt aaaaggttcc cgatctaccc gtaaagcttt ggtgattaaa ggtcttttcg
     4561 taaagctcaa ggatactctg tcggtctgct tgtggtatcg cctcgctaag tgtattaaaa
     4621 gctgcttcca taacctgggc gtttctgaag gaagggttaa aatggcaggc tctctgtgag
     4681 tcctggaaca taatggaggt gttacccgag gcaagcagtc gggcgagcga tgggatgcgg
     4741 agggcatctt cttcgttgac gatattcttg gcgcaaaaat gtggaagttc cttgttgggg
     4801 tacaggatgt aggaatactg gtccttgcgg aagagcggtc ctttcttcat ggccttgcac
     4861 aagtcaagca cttcttttga actgagtaag agcgaagaga tgaccgctac ctgcccctcg
     4921 agcatttcgt tcaggtactg tacgtccatg ccgccagaag tggcaatgga gagggtgttg
     4981 tacgcatggt aaagaccatc ctgtcgtttg ttggctcgga tcgtctgttc ggatgcagtg
     5041 cggaacaca
//
LOCUS       DAOQXQ010000028         4949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_17310_length_4949_cov_3.015938, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000028 DAOQXQ010000000
VERSION     DAOQXQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17310_length_4949_cov_3.015938"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /gene="mutS"
                     /locus_tag="PKH81_00860"
     CDS             <1..1534
                     /gene="mutS"
                     /locus_tag="PKH81_00860"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNQ96627.1"
                     /translation="LDLERLSSRVAMDRCHPKDLAAIRQSLSSYVSLLDVIQDEGVSN
                     IVEHSFREAVIPLVTLLEKALEDDPPLQISDGSVIRSGWSKRLDELRELKDNTNVVLE
                     RYLESEREETGIGNLKVRYNRMLGYYLEVSKGNLDSVPDHFIRRRSLANGDRYTTDRL
                     VALEVEINGVAASIAETEQDLFLSLKKEIALNLETLQSVARVLAEIDVFQSFAQGATV
                     NGWVRPSFTDSGLMDIQDGRHPVVEAHVGQGDFIPNSITVNSDRSGISFAMITGPNMA
                     GKSTFLRQTALISLMAQCGSFVPAQSAIITPLDRIFCRVGASDNLARGESTFLVEMTE
                     TAYILRSATKDSLVIMDEVGRGTSTEDGLSLARSITEFLLDTIGAKTLFATHYHELSR
                     INHPRLKNFCLDVLETEGTVVFLKKVIEGASANSYGLHVARLAGIPEQVILRAKDILS
                     SQSPASHVAIVPEKAHPSADISLFPEEDLVLNELLSVDVNTLTPIDALALIHRLQQRL
                     QP"
     gene            1587..2315
                     /locus_tag="PKH81_00865"
     CDS             1587..2315
                     /locus_tag="PKH81_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96628.1"
                     /translation="MREHLVEYAISHCFSSSCLLCGRQSSRPLCSDCLDINYLDKIDG
                     LSLLRDSPHRCAHCGRPLISRNKLCTSCLTLDVLSVIDRVLPLFYYSPANSLLLNSWK
                     THGQRGLGYPFARMMSIPILETLARDKDMIIVPVPPRPGKIREKGWDQIEELGTLLQR
                     YWKLPVKSILRRTATMQQKKLNRAGRLSNLKGCITAKKSLVGISSALVIDDIMTTGAT
                     LESCAAALKESGVSRVYGCTLFFD"
     gene            2481..2951
                     /gene="rimP"
                     /locus_tag="PKH81_00870"
     CDS             2481..2951
                     /gene="rimP"
                     /locus_tag="PKH81_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNQ96629.1"
                     /translation="MEYIAPEGISHYADCEPLVSGLGYSLVELVVYTRQSAWHVKVVI
                     TSPQGVGIKDCSTVHRALLPRLEAVLQSQDMYVEVTSPGLDRVFKNASEFAVFAGRLV
                     KIWDTDISDWVSGTIVSATTESVILATQDGERVYPYTKIAKAKLAGTQTAQGKQ"
     gene            2966..4450
                     /gene="nusA"
                     /locus_tag="PKH81_00875"
     CDS             2966..4450
                     /gene="nusA"
                     /locus_tag="PKH81_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNQ96630.1"
                     /translation="MSAEMAEAIRQLVQEKGIDEDLVFRMIEETLKAAYKRKFGMNDN
                     AVVTFNDAHTDVSIYSRKTIVDGVYNPVIEIELEEALELDPECEIGDELLIEVDPKEF
                     DRLSVQSAKQTAHQSIRDIQKDSLHSEYKDKVGEIIIGYYQRERNGNIYVDLGKVEGI
                     LPKKLQSPRETYRQNDRIKALITEVKKTPSGLQIVLSRTDPDFVRRIMELEVPEIYDK
                     TVEIHKIVRESGYRTKVAVFSHRDDVDPVGACVGLKGVRIQAVIRELEGEKIDVLKYD
                     EDPRAFISNALSPAEVSDVVILDEGKRSALAIVAEPQFSLAIGKQGLNVRLANRLADW
                     NIDVKTIEQFEEMDITADSRRAANDLFSDSEDIEEISAICELPGITEDMVALLEQSNI
                     TRIEQLLSMSDEQIIALEGMTDERYQSLSRMLKESLDVVDEVPVTPATADAVEESGIS
                     TQAEEVVAEKEEYECPECGAPITIDMTTCPNCGIGLSFEYEDEE"
     gene            4459..>4949
                     /locus_tag="PKH81_00880"
     CDS             4459..>4949
                     /locus_tag="PKH81_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96631.1"
                     /translation="MAEDLENTQKPKVILNKQKKPDPASQEKEGVAPAPDQGEKKKVV
                     VIKKKSASKDGPQSGSNDTGETPEHTHVKRTVHVVPVHKDKGEAQTDIPVAKQGDSEQ
                     KQKPTSIELSPPRPNVKVGNLADHSRSQPRQGGYQGRSGGYQGQRDGFSGAQAREGAR
                     DPSA"
BASE COUNT         1329 a         1058 c         1183 g         1379 t
ORIGIN      
        1 attggatctt gaacgacttt catcccgagt tgctatggat cgctgtcatc caaaagattt
       61 ggctgctatc aggcagagcc tttcctcata tgtatccttg ttggatgtga tacaggatga
      121 gggagttagc aacatagtgg aacattcttt tagggaagcg gtaatccctt tggtgaccct
      181 tctggaaaag gctctggaag atgatcctcc actacaaatc agtgatggaa gcgttattcg
      241 ctctggttgg tctaagcgcc ttgatgaact tagagagctc aaggataata ctaatgttgt
      301 ccttgagcgc tatctggaga gtgagcgaga agagacgggg attggcaatc ttaaggttcg
      361 ctataaccgt atgcttgggt attatcttga agtaagcaag ggtaaccttg actcagttcc
      421 ggatcatttt atccgtaggc ggtcccttgc taacggggat cggtacacaa cagaccggct
      481 ggttgcactt gaggtggaaa ttaatggagt ggccgcttct attgcggaaa ctgaacaaga
      541 cctttttctt tccctaaaga aagaaatcgc attgaatctt gaaacattac aatcggttgc
      601 acgtgtgttg gcagaaattg atgtatttca atcttttgcg cagggagcta ctgttaatgg
      661 ttgggttcga ccttctttta ccgatagcgg tttgatggat attcaggatg gtcgccatcc
      721 agttgttgaa gctcatgtag gtcagggtga ttttattcca aacagcatca ctgtcaattc
      781 tgatcgttcc ggtatctctt ttgccatgat taccggacca aacatggccg gtaagagtac
      841 ttttcttcgt cagactgcat tgatatccct tatggctcaa tgcggatcct ttgttcccgc
      901 tcaaagcgcc ataattacac cattggacag gatcttttgc agggtaggtg cctcggataa
      961 tcttgccaga ggagagtcaa cctttttggt agaaatgacc gaaacagcct atattctccg
     1021 ctctgcgaca aaagacagct tggttatcat ggacgaggtt ggccggggta cctctactga
     1081 agatggattg tccttagccc gatcaataac cgaattcctt cttgacacaa tcggtgcaaa
     1141 gactctgttt gccactcatt atcatgaact gtctcgaatc aatcaccctc gcttaaagaa
     1201 tttttgtctt gatgtgcttg aaacagaggg taccgtcgtt tttttaaaga aggttattga
     1261 gggcgcttca gccaactcat atggcttaca tgttgctcgt cttgccggta tccctgaaca
     1321 ggtaatcctg cgggcaaagg atattctttc ctctcagtcg cccgcaagtc acgtcgccat
     1381 cgtccctgaa aaagctcacc cttctgctga tataagtctt ttcccagaag aagacctggt
     1441 tcttaatgaa ctactcagtg tggatgttaa tacccttact cctattgacg cccttgctct
     1501 gattcatcga ctccagcaac gattacaacc ttaaaaaaag tgccaccctt ctcgttttta
     1561 tgtacacagt ttgcatgata actcccttga gggaacatct tgttgaatat gccatttctc
     1621 attgttttag ctcttcttgt ctgttatgcg gaaggcaatc atcacgtccc ctgtgttcag
     1681 attgcctcga tatcaattat cttgataaaa ttgatggatt atcgctcttg cgggactctc
     1741 cgcatcgatg tgctcattgc ggtcgccctc ttatctcccg aaacaagctt tgtacttctt
     1801 gtcttactct tgatgttctt tcggttatag atcgggttct tcccttattt tattatagtc
     1861 cggccaactc gctgttatta aattcctgga aaactcacgg acagcgaggt ttgggctatc
     1921 cctttgcccg aatgatgagt atacccattc ttgaaacact agcacgggac aaagacatga
     1981 tcattgttcc tgtcccaccc cggcctggta aaatcaggga aaaaggatgg gatcaaatag
     2041 aagagttagg gactctttta cagcgttatt ggaaattgcc ggttaagtcg attctaaggc
     2101 gtactgctac catgcaacaa aaaaaactga atagggcagg gcgtttatca aatctgaaag
     2161 ggtgcattac cgcaaaaaag tctcttgttg gtatttccag tgccctggta atcgatgata
     2221 ttatgactac gggggcaacc cttgaatcct gtgcagcagc gcttaaggaa agcggggtat
     2281 ctcgtgtata tggatgtacg ctcttctttg attgacagtc cataaagagg gctctgcttg
     2341 acattacgcg gtctgtatgc taatttcttt attgaacatt tatcatcctt tattcgtcag
     2401 gagtcgttca aacggctcct ttttcgtata cggaccgtaa aggaccgctg atcttattca
     2461 gcacagggag ttgcagggta gtggagtata ttgcgcctga gggaatttca cattatgctg
     2521 attgtgagcc gctcgtcagt ggtttggggt acagcctggt tgagctggtt gtgtacacgc
     2581 gtcagtcggc atggcatgtg aaagtagtaa taacctcacc acaaggcgtt ggtattaagg
     2641 attgttccac tgtccatcgc gccttgcttc ctcgtcttga ggcggttctt caatcccagg
     2701 atatgtatgt agaagttacc tctcccgggc ttgacagggt gttcaagaac gccagtgagt
     2761 tcgccgtctt cgcaggaagg ctggttaaaa tatgggatac tgatatatcg gactgggtgt
     2821 ctggtacaat cgtctcggca accaccgaat cggttatact tgcaactcag gacggagagc
     2881 gggtttatcc ctataccaaa attgcaaagg ccaaacttgc cggaacgcaa accgcgcagg
     2941 gcaagcagta gagaaggagt ttttcatgtc cgcagaaatg gcagaagcga tacgccaact
     3001 cgttcaggaa aaaggaattg acgaagatct tgtattcaga atgattgaag aaaccctcaa
     3061 ggccgcttat aagcggaaat tcgggatgaa tgataatgcc gtcgtaacct ttaatgatgc
     3121 tcataccgac gtatccattt attcacgtaa aactatcgtt gatggggtgt ataatcctgt
     3181 tattgagatt gaacttgaag aagcactgga attggatcct gaatgtgaaa ttggagacga
     3241 attgctcatt gaggttgatc caaaagaatt cgatcgtttg tcagttcagt ctgccaagca
     3301 aaccgcacat cagtccatta gggatattca aaaggatagt cttcattctg aatataaaga
     3361 taaggttggc gagataatta ttggctatta tcaacgagag cgtaatggta atatctatgt
     3421 ggacctgggt aaggttgaag gtattctccc taaaaagctt caatctcctc gcgaaacgta
     3481 tcgtcaaaac gatcgtatta aggccttgat taccgaggtt aagaaaaccc cctccggtct
     3541 tcaaattgtg ttatccagaa ctgatcccga ttttgtccga cgtattatgg aacttgaggt
     3601 tccagagatt tatgacaaga ccgtagaaat ccacaaaatt gtccgcgaaa gtgggtatcg
     3661 gacaaaggtt gccgtttttt cccaccggga tgacgttgat ccagttggcg cctgtgttgg
     3721 gttaaagggc gtgcgtatac aagcggtaat tcgtgaactt gaaggcgaaa aaattgatgt
     3781 tcttaaatac gatgaagatc cccgagcctt tattagtaat gcgctttctc ctgccgaggt
     3841 gtctgacgtt gtaatacttg atgagggaaa acgatcagcg cttgcgattg tcgctgagcc
     3901 acagttttcc cttgcgatcg gtaaacaagg tcttaacgta cgtctggcaa accgtctggc
     3961 ggattggaat atcgatgtta aaaccatcga gcaatttgag gaaatggata ttactgctga
     4021 ctcacgaaga gccgctaatg atctgttttc tgattcagag gatatcgagg aaatttctgc
     4081 gatatgtgag cttcccggta ttaccgaaga catggttgct ttgcttgaac aaagcaatat
     4141 aaccagaatt gaacaacttc tttccatgag tgatgagcaa atcattgcgc tggaagggat
     4201 gaccgatgag cgctatcaat ctctttcacg tatgcttaag gaaagccttg atgtcgttga
     4261 tgaagttccc gtaactcctg ctacagctga cgctgttgaa gaatccggaa tttcaacaca
     4321 ggctgaagag gttgttgcag aaaaagaaga atacgagtgc ccagaatgtg gtgcccccat
     4381 aacgatcgat atgacaactt gtcccaattg tggtatcggt ctcagttttg aatacgagga
     4441 cgaagaatag gatactgtat ggctgaagat cttgaaaaca cccaaaaacc gaaggtaatt
     4501 ctcaacaagc aaaaaaaacc tgaccctgcg tcgcaggaga aggagggtgt tgctcctgcc
     4561 cctgatcagg gtgaaaaaaa gaaagtcgtc gtcatcaaga agaaaagtgc ttcaaaagat
     4621 ggtcctcaat ctggttccaa tgatacgggc gaaacacccg agcatactca cgtaaagcga
     4681 accgtgcatg ttgttcctgt tcataaggat aagggcgaag cacaaacaga tattcctgta
     4741 gctaaacagg gagatagcga gcaaaagcaa aaacctacct caatagagct cagcccacca
     4801 agaccaaatg ttaaagtggg taatcttgcc gatcattcac gttcccaacc ccgacagggt
     4861 ggctatcagg gtcgtagtgg tggatatcaa ggtcaaagag acggtttctc cggcgcgcaa
     4921 gcaagggaag gagcacgtga tccatctgc
//
LOCUS       DAOQXQ010000029         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_17456_length_4920_cov_3.162795, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000029 DAOQXQ010000000
VERSION     DAOQXQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17456_length_4920_cov_3.162795"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..904
                     /locus_tag="PKH81_00885"
                     /pseudo
     CDS             18..904
                     /locus_tag="PKH81_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
     gene            948..1565
                     /locus_tag="PKH81_00890"
     CDS             948..1565
                     /locus_tag="PKH81_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNQ96632.1"
                     /translation="MDVQLQELVDRIKKDGVASADAEASRIRQEAQKEAEHILKNAKA
                     EAEKLIQEAQKDADRLESVAVSSIKQSGRNILISFRDSVTTEISTLIKNGVSSALSAD
                     TLKELVPSVVKAWASRSSVDDLAVLLSKEDMTKLESSLVSTLKAEISAGLELTSDGSL
                     SSGFRLGMKDGSAYYDFSAEAVASLFSAYLNPRVAQILASAAKEL"
     gene            1569..2120
                     /locus_tag="PKH81_00895"
     CDS             1569..2120
                     /locus_tag="PKH81_00895"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNQ96633.1"
                     /translation="MSRYYYLMAQLPAILPNNPLALSYASFLELAGRFLSKKDIRLLN
                     RLSLEPERNPETVPSSLVQLWYDRERSLRLSLARLRMTKLGRDASLRGSDEQVLLAGP
                     DMQGVARAAFAMDNPLDAEQFLDTTRFSWIEELSRGHYFDSEAVFAYALKVLIRERGN
                     RFNDSAGRESYTSIYTEILGEEA"
     gene            2117..3904
                     /locus_tag="PKH81_00900"
     CDS             2117..3904
                     /locus_tag="PKH81_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNQ96634.1"
                     /translation="MTDTKGSVVAVNGNMISVRFDGIVTLNEVGYVKVGDQKLKSEII
                     RIRGDVAQLQVFEITKGIQIGDTVEFTGDLLSVELGPGLLGQVYDGLQNPLPELASKA
                     GYFLERGLYLDPLPDSTRWDFTPGATVGSRVVRADTLGTVPEGAFTHRIMVPFTMYGT
                     YTVKSIKEKGNYALKDVIAEITDENGKVYPLTMSFEWPVKRAIDCYAERLKPTETLVT
                     KTRTIDTFFPVAKGGTYCIPGPFGAGKTVLQHTTSRHADVDIVIIAACGERAGEVVET
                     LKEFPELIDPKTGRSLMERTIIICNTSSMPVAAREASVYTGVTLAEYYRQMGLHVLLL
                     ADSTSRWAQALREMSGRLEEIPGEEAFPAYLESYIAAFYERAGIVRLRDGTKGSVTIG
                     GTVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIHPLDSWSKYSGVMDVEW
                     VEFARTYLRRGNEVEQMMKVVGEEGTSLDDFIVSLKGSFLDAVYLQQNSFDEIDAAVS
                     TERQSHIFSMILKILGTSFTFKNKDEARSWFNTTRLLFIDYNYSPWESQQFKKHELEI
                     IAAVEEKSDGLDPVVEKMLGRTSSARVTE"
     gene            3908..>4920
                     /locus_tag="PKH81_00905"
     CDS             3908..>4920
                     /locus_tag="PKH81_00905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ96635.1"
                     /translation="MKKVYSKIESINGSVITVKADGVKYGELAEIETSFGTSLAEVNK
                     LSGDLVSLQVFAGGRGVSTGDKIRFLGNQMRVSFSENLLGRIFTGSGNPRDGGPTLQD
                     NLVQIGGPSVNPSKRIIAKRMIRTGIPMIDMFNTLVVSQKLPIFSSSGEPYNELLARI
                     AMQAEVDVIVLGGMGLKYDDYLYFKDTLENAGALSRTAMFVHTAADPTVECLMIPDMC
                     LAVAEQFAIQGKDVLVLLTDMTNFADAMKEIAIIQEQVPSNRGYPGDLYSQLAARYEK
                     AVDFDDAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNGRIEPFGSLSRLKQN
                     VNG"
BASE COUNT         1251 a         1139 c         1195 g         1335 t
ORIGIN      
        1 ccggcaaaga tggaagcatg gaagatgttg ccttgggcta ttcaacactg gatgggtatg
       61 tacataaccc agtttttttt ggttgtatag ttgggcgtta tgctaatcgc atagccggtg
      121 cctcttttac tctgacggac aaacccatcg actaactgct aatgacgggc ctaattgcct
      181 gcattcaggg cgtccggggt atcacaagat ggtctgggaa gctactcctt tttgcaaatc
      241 ctccgaggcg ggagtcgttt ttactcgtgt aagcccggcg ggcgagcagg gtttcccggg
      301 aaattgcgat cttaaggtat cgtacagttt aaccgcagac gataatatcg ccattaagta
      361 tcttaccaga acagacgaag cttgcccagt taatctgact aatcacacct attttaatct
      421 ctctggcgcc ggatcgggaa ctatactcga tcacagtgtg caactttttg ccgatcacta
      481 tctaccggtg gatgctcagg caattcccac aggagacctt gcgccagtag aagggactcc
      541 cttcgatttc cgcactccca aggctatcag gcgagatata gaggccgtaa ctggaggtta
      601 tgaccataat tggtgcgtta accgggatga ctcctcctgc gctcccgttg ccggggtgta
      661 tgaaccactt tccggcaggg cgttaaccgt ctattcgaca caaccgggtg tgcagttgta
      721 tactggcaat tttcttaccg gggaaaaagg aaaaaacgga gatgtatatg gcaagcatgc
      781 cggtttctgc cttgaaacac agcattatcc cgattctccc aatcgacctg catttcctga
      841 taccgttatt cttccgggag aacgatataa tcacgaaact atttggcact tcgattataa
      901 ttagcaatta ctgacacggt gatttacact atttggaagg ggttaatatg gacgttcaat
      961 tacaggaact ggttgacaga atcaaaaagg acggggttgc ctctgctgat gctgaagcct
     1021 ctcgtattcg ccaggaagct cagaaagaag ccgagcacat acttaaaaat gcaaaggctg
     1081 aagcagaaaa actgatacaa gaagcgcaaa aggatgctga tcggctagaa agcgttgcgg
     1141 tttcttcaat caagcagtca gggcgtaata tcctcatttc cttcagggat tcggtaacta
     1201 ccgagatttc cacccttatt aaaaatggtg tttcttctgc tttatcagca gataccctaa
     1261 aggagcttgt tccctctgtt gtcaaggcct gggcttctcg ctcttcagtt gatgaccttg
     1321 cggtgctgct ttcaaaagaa gatatgacaa aacttgagtc ctcccttgtt tcaaccctta
     1381 aagcggaaat aagtgcaggt cttgaactaa cctccgatgg cagcctttca tccggttttc
     1441 gcctgggcat gaaggatggt tcggcatatt atgatttttc cgctgaagcg gtcgcctctc
     1501 ttttctcggc atatcttaat ccccgggtgg cacagatact cgcgtcggcc gcgaaggagt
     1561 tataagcggt gtcccggtat tactacttga tggcacagct tccggctatt cttcccaaca
     1621 atcctctcgc tctttcctat gcttcttttt tagagcttgc aggacgattc ctttcaaaaa
     1681 aggatattcg tttgttaaac cggctgtccc tggaacctga gcggaatcca gaaacagttc
     1741 catcatccct tgtccagctg tggtatgacc gggagcgctc tctcaggctt tctcttgcgc
     1801 gcctgagaat gacaaaatta ggtcgtgacg catcattacg aggtagtgat gaacaagtcc
     1861 ttctagccgg tccagatatg cagggtgtag ccagggctgc ctttgccatg gataacccgc
     1921 tcgacgccga acagtttctc gatacaaccc gtttttcctg gatagaagaa ctttcacggg
     1981 gacattattt cgactcagag gcggttttcg cctatgctct taaggttctt atccgcgaaa
     2041 gaggtaatcg ttttaacgat tccgctggac gcgagtctta tacatcaatt tataccgaga
     2101 ttcttggaga agaagcatga ctgatacaaa aggcagtgtt gtcgccgtta acggcaatat
     2161 gatcagtgtc cgctttgacg gcatcgttac cctaaacgag gttggatatg tcaaagtagg
     2221 cgatcaaaaa cttaagagcg agataatccg cattcgtggc gatgtcgcac aacttcaggt
     2281 ttttgaaatt acaaagggaa tccaaattgg cgatactgtc gagtttaccg gagatctttt
     2341 gtcggttgag ctcggtccgg gacttctggg acaggtatac gatggcttac aaaatccgct
     2401 tccggaactt gcaagcaaag ctggctactt tttggagcgg ggcttgtatc tggaccccct
     2461 tcctgattcc actcgatggg acttcacccc gggagccacc gtgggctctc gcgttgttcg
     2521 cgcagatacg ctgggaacag ttccggaagg agcctttact caccgcatta tggttccctt
     2581 taccatgtat ggtacctaca ccgtcaagag tatcaaggaa aaagggaatt acgcccttaa
     2641 ggatgttatt gcagaaataa ctgatgaaaa cggcaaggtc tatcccctta ccatgagctt
     2701 tgagtggccc gttaagcgag caatcgattg ttatgctgag cgacttaaac cgacagaaac
     2761 cctggttacc aagacccgga ctattgatac cttttttccc gttgccaagg gtgggacgta
     2821 ttgtattccc ggtcctttcg gtgcgggtaa aacggtatta caacacacca caagccgtca
     2881 tgctgatgtc gacatcgtta ttattgcagc atgtggggag agagcaggtg aggttgtaga
     2941 aactcttaag gagttccctg agcttatcga tcccaaaaca ggtcgctctc ttatggagcg
     3001 gacgattatc atttgtaata catcatccat gccggtcgcg gcacgagagg catctgtata
     3061 taccggtgtt actcttgctg aatactatcg acaaatggga cttcatgtgc ttctccttgc
     3121 cgattctacc agccgttggg cgcaggctct gcgagagatg tctggccgtc ttgaagaaat
     3181 tcccggagag gaagccttcc ccgcctatct tgaatcgtat atcgccgctt tctacgagcg
     3241 agccggtatt gtacgactga gagatggtac aaaaggatcg gtcaccatag gtggtactgt
     3301 ttctcctgct ggtggtaact ttgaagagcc ggtaacacag gcaaccctta aggttgttgg
     3361 tgcattccat ggactttccc gtgaacgttc cgatgcccgt aaatatccgg caattcatcc
     3421 gcttgactca tggtcaaagt attcaggagt catggatgta gaatgggtag agtttgctcg
     3481 tacatacctt cgtcgtggaa atgaggtaga gcagatgatg aaggtcgttg gagaggaagg
     3541 aacaagcctt gacgatttta tcgtaagtct taagggaagc tttcttgacg cagtatatct
     3601 gcagcaaaac tcatttgacg agattgacgc agccgtatct actgaacgac aaagtcacat
     3661 cttttcaatg attcttaaga ttcttggcac ctcatttact tttaaaaata aggatgaggc
     3721 acgcagctgg tttaatacca cccgtcttct ttttattgac tataactatt caccttggga
     3781 atctcagcaa ttcaagaaac atgaactgga aatcattgcc gccgtcgagg aaaaatccga
     3841 tggtcttgat cccgttgtcg aaaaaatgct tggtcgcact tcgtcggcca gggttacgga
     3901 gtaagccatg aaaaaggtat acagcaaaat tgaatcaatc aacggttcgg taatcaccgt
     3961 aaaggctgat ggggtaaagt acggggagtt agccgaaata gaaacttcct ttggcacatc
     4021 ccttgccgag gttaataaac tttccggaga ccttgtatcc ctgcaggttt ttgccggtgg
     4081 acgcggtgta tccaccggag acaagatacg gtttcttgga aaccagatgc gtgtgagctt
     4141 ttcagagaat ctcttgggac gtatctttac cggctcaggt aatccacgag atggagggcc
     4201 aacacttcag gacaatctgg ttcaaattgg tggcccttct gtaaatcctt caaagcgtat
     4261 aatcgccaaa cggatgattc gcactggtat tccaatgatc gatatgttca atactttggt
     4321 tgtatcccag aagttgccca ttttttccag ttcaggagag ccttacaatg agttgctggc
     4381 gcgcatcgcg atgcaggccg aagtagatgt aatcgttctt ggtggtatgg gacttaagta
     4441 tgacgattac ctttatttta aggataccct cgaaaatgcc ggagctctta gccgtacagc
     4501 catgtttgtt cataccgcag cagatccaac cgttgaatgt cttatgattc cggatatgtg
     4561 tcttgctgtc gccgaacagt ttgctattca agggaaagac gtgttggtac tacttaccga
     4621 catgacaaac tttgctgacg cgatgaagga aattgcaatt atccaggaac aggttccctc
     4681 aaaccgcggc tatccgggag acctgtatag tcagcttgcc gcccgttacg aaaaagccgt
     4741 tgattttgat gacgcaggct cagtaaccat tcttgcagtt acaaccatgc ctggagacga
     4801 tgttactcat cctgttccgg acaatacagg atacataacc gaaggacagt tttatcttaa
     4861 aaacggtcgt attgaaccct ttggaagtct ttcccgtctt aagcaaaacg ttaatggtaa
//
LOCUS       DAOQXQ010000030         4885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_17616_length_4885_cov_4.103313, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000030 DAOQXQ010000000
VERSION     DAOQXQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17616_length_4885_cov_4.103313"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..267)
                     /locus_tag="PKH81_00910"
     tRNA            complement(182..267)
                     /locus_tag="PKH81_00910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Ser,seq:gga)
     gene            complement(464..548)
                     /locus_tag="PKH81_00915"
     tRNA            complement(464..548)
                     /locus_tag="PKH81_00915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Ser,seq:cga)
     gene            complement(600..673)
                     /locus_tag="PKH81_00920"
     tRNA            complement(600..673)
                     /locus_tag="PKH81_00920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(637..639),aa:Arg,seq:gcg)
     gene            complement(756..843)
                     /locus_tag="PKH81_00925"
     tRNA            complement(756..843)
                     /locus_tag="PKH81_00925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Ser,seq:gct)
     gene            complement(898..982)
                     /locus_tag="PKH81_00930"
     tRNA            complement(898..982)
                     /locus_tag="PKH81_00930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(946..948),aa:Ser,seq:tga)
     gene            1071..1907
                     /gene="thyX"
                     /locus_tag="PKH81_00935"
     CDS             1071..1907
                     /gene="thyX"
                     /locus_tag="PKH81_00935"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNQ96636.1"
                     /translation="MAHCIVPSAEEILDKEYPVLDKGFVRLVDYMGGDSRIVQSARVS
                     YGEGTKTVREDGALIDYLLRNEHTSPFEQVVLTFHIKLPIFVARQWIRHRTARVNEIS
                     GRYSVMKDECYVPAEADVAFQSTDNKQGRATEAPPAALVQEVRTTLENQQKFVFDGYR
                     DLISQNIAKELARINLPLSTFTEWYWQIDLHNLFHFLRLRCDGHAQKEIRDYAFVMLD
                     ICKRVAPLATESFERHQMNGVHFSQAELTALRALLQGKETGLAGKDLERFNEKLTNGR
                     QI"
     gene            complement(1904..3616)
                     /locus_tag="PKH81_00940"
     CDS             complement(1904..3616)
                     /locus_tag="PKH81_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96637.1"
                     /translation="MSDLQMQGFGGLPAASEKDLLAMNQLMYDKQYRIKVSPGPLRVE
                     KTDKVNPEFIQYILNDYLGHVQKCVAETRKMLPYEDQKFFYEAFHQQDPVGYSAMLAI
                     SSFDIRALGELLSRLQANPREVTVSQLSPFVRLLYRMLIRFYYLGSQGISRLYRSVHS
                     LIVRELVPADPDSLKMHVTAAITEWVYILDSVCTGLYPLLLRMSSPVMLTMRQLMYDN
                     GSKVLAWLQIAPSEVIFQKDLAPLVADGSASVRVAEKEPEEDFSLPDEVTQGLDILET
                     LFPEAGWDDLESMPDLCPYFQPVLQFQDAFTQLAPDNPLHQTMIFFWILEELFQGLRL
                     IKFEPLPATSVKDDVEDINRILEDWILYQESVFDKNFAVELKSYTHQIYTQPDFHKSP
                     YGRKLLSNMYTLIKTMFLPYFDIKMYGTTRGPKDDRLPPFYVRVRRLKRLLSRYDAAI
                     ELAPEGSDQNPEGRVPGVVNPWSPYKFDIANPVSKRLDALCGGKHAKGRTNALLIHYT
                     LSILNVLDWWINNKDSYAYTIPCEYLYRVIEPGSAVPAFGVTPRTDVDQIFQRSLKAR
                     LSGD"
     gene            complement(3657..4763)
                     /locus_tag="PKH81_00945"
     CDS             complement(3657..4763)
                     /locus_tag="PKH81_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNQ96638.1"
                     /translation="MVLFRKQKRWYNRPTRCVLYTREILPAPEYPVENGKPVFGTFQG
                     PFGHFDIRGMKRPFGNLPIPELITNRRIMGIMRFLFCDDRHIGEIELFDAGYFSFMET
                     TLWNRTTGRRIAYRRIIPPGFMKMPIEFSNSVTSCRSRSRFTRVHMRLQKKIIHADFD
                     FLGSWARPPCEGRFEMDLSAPGAADLSAVTPYRVRRRCQASFQQTAALQGWIGTGYDD
                     YRIPLSSGVGFFDVRKAYFSLRTKMSRMIGMGRLDGKIVSFQIGNSVTREDLKYNDNV
                     LFVDGTSWPLPPVKITRPYGVGGVWIIQDTESMVDLTFTPISDSARKLSALVLRTDYH
                     TVYGTYDGVLLTGDGTEIRLKGYPGIGKKILLRI"
BASE COUNT         1352 a         1297 c         1089 g         1147 t
ORIGIN      
        1 aaccgccatg atggttgctc ggcttattct tgctgtacgg agagaggggg attcgcccct
       61 tcggggccgc cctcgtccaa agctgcgctg gctcgctttg gactcacgat cgaatcccca
      121 aacaacactc tttgctaacg aataaaccgc catgatggtt gctcggctta ttcttgctgt
      181 acggagagag ggggattcga acccccggta ccttttgggc acacacgcct tccaagcgtg
      241 caccttcgac cactcggaca tctctccaaa gataaagaaa acccatgtaa aaaagagcga
      301 agcttgcctc tcacgaaagc ggagagaggg ggattcgccc cttcggggcc gccctcgccc
      361 gaagctgcgc tagctcgctt cggtctcacg atcgaatccc caaccagctc actttccagt
      421 gcccaaaccg ccgtattatg taacggcttg ttgctcacga aagcggagag agggggattc
      481 gaacccccgg taccttgcgg tacaacggtt ttcgagaccg cccgattcag ccgctctcgc
      541 atctctccaa tacagaaagg ttctctgtca aaaaaaggct gtctgaaaca gacagcctat
      601 gagactaagt gggttcgaac caccggcctt cagatccgca atctgacgct ctatccagct
      661 gagctatagt ctctcaatcg atcgcttcgg tctcacgatc gaatccccca tcaggcagga
      721 ttcttataag ctgacacgca aagtgtcata cttaacggag caggggggat tcgaaccccc
      781 ggtacccttt tggggcacaa ctccttagca gggagcccga ttcggcctct ctcgcaccgc
      841 tccaaggata acgttttccg gacagactgt ccacggacaa tcttggcgtc ctcaagccgg
      901 agcagggggg attcgaaccc ccggtacctt tcggcacaac ggttttcaag accgcctcct
      961 taaaccgctc ggacaccgct ccaaaaaaat gttctttaca atagcgcaaa gcactacttg
     1021 atgtcaagat tcccttgaga taacgctcaa tgacgtatat acttgcccac atggcacatt
     1081 gtatcgttcc ttcggcagaa gaaatcctgg acaaagaata cccggtattg gacaagggct
     1141 ttgtacggct tgttgactac atgggcggag attcccgcat cgtccaatca gcgcgtgtat
     1201 catacggaga gggcaccaaa accgtccgcg aagacggagc tcttatcgac tacctcttac
     1261 gtaatgaaca tacctccccc tttgaacagg ttgtccttac ctttcatatt aaacttccca
     1321 tctttgtggc caggcagtgg attcgccacc gaaccgcccg tgttaacgag atatcaggtc
     1381 gctattccgt tatgaaggac gaatgctatg ttccggcaga ggcagatgtt gcttttcaaa
     1441 gcactgataa caaacaaggt agagcaaccg aagccccgcc tgcagccctt gttcaagaag
     1501 ttagaactac cttggagaac caacaaaagt ttgtgtttga cggatatcgc gaccttatct
     1561 cgcaaaacat tgccaaagaa ctggcaagaa taaaccttcc cctgtctacc tttaccgaat
     1621 ggtattggca aatagactta cataacctct tccattttct taggctcaga tgcgacggac
     1681 acgcccaaaa agaaattcgc gactatgcct ttgttatgct cgacatctgc aaacgtgttg
     1741 cgccattggc aacagaatcc tttgaacgcc atcaaatgaa cggcgttcat ttctcgcaag
     1801 cggaactcac cgccctaagg gcattgcttc aggggaaaga aaccggtctt gccggcaagg
     1861 atctggagcg cttcaacgaa aaacttacca acggacgaca gatttaatct ccagaaagtc
     1921 gcgctttaag cgacctctga aaaatctgat ccacgtccgt tcgcggggta acgccaaatg
     1981 caggaaccgc actccctggc tcaattactc gatacaggta ctcacaagga atggtatagg
     2041 catagctatc cttgttgtta atccaccagt caagcacatt caatatagaa agggtgtagt
     2101 ggataagcaa ggcatttgtc cgcccctttg cgtgctttcc accgcacagg gcatcaagtc
     2161 gtttggacac cggatttgca atatcaaatt tatacggact ccagggatta accacaccag
     2221 gcacccttcc ttcgggattt tggtcacttc cctccggagc aagctcaatc gccgcatcat
     2281 agcggcttaa cagacgctta agccgacgaa cacgaacata aaagggaggc aaccggtcat
     2341 cttttggccc tcgtgtagtt ccatacatct tgatatcaaa atacggcaag aacatcgttt
     2401 ttatcagagt gtacatgttg gaaagaagct tgcgcccata cggcgattta tgaaaatcag
     2461 gttgcgtata aatttggtga gtatacgatt ttaattccac cgcaaagttt ttgtcgaata
     2521 cgctctcctg atacaatatc cagtcttcta aaatgcgatt aatatcctct acgtcatcct
     2581 taaccgaggt cgcaggcagc ggttcaaatt ttatcaagcg taaaccttga aacagctcct
     2641 ccaaaatcca gaaaaagatc atggtttgat gcaggggatt atctggggca agctgggtaa
     2701 aggcatcttg aaactgtaag actggctgaa aatacgggca cagatccggc atgctttcaa
     2761 gatcatccca accagcctct ggaaatagtg tttcaagaat atcaagccct tgcgtaacct
     2821 catccgggag agaaaaatct tcctcgggct ctttttccgc aacacgtacc gaggccgacc
     2881 cgtcagcaac aagcggagcc aagtcctttt gaaagataac ctcggaaggc gcgatctgaa
     2941 gccatgccag aaccttggag ccgttatcat acataagctg gcgcatggtc aacattaccg
     3001 gagagctcat ccttaaaaga agcgggtaaa gtccggtaca cactgaatcc aggatataca
     3061 cccactctgt aatagccgca gttacatgca tcttaaggga atcagggtcg gcagggacca
     3121 attcacgaac aatcaatgaa tgaaccgaac gatacaagcg ggaaatcccc tggcttccca
     3181 aataataaaa acgaatcaac attcgatata aaagccgtac aaaaggagaa agctgggaga
     3241 cggttacctc ccgaggattt gcctgcagac gcgacaacaa ctccccaagg gcgcgtatat
     3301 caaaagaaga aatcgcgagc atcgcagaat accctaccgg atcctgttga tgaaaggctt
     3361 cgtaaaaaaa cttttgatct tcgtagggca gcattttgcg tgtctctgca acacattttt
     3421 gaacatgccc caaataatca ttaagaatat actgaataaa ttcagggtta accttgtctg
     3481 ttttttcaac tcgcaagggc ccgggagaaa ctttgatgcg gtactgcttg tcatacatca
     3541 gttgattcat cgccaacaag tctttctcgg acgcggcggg aagaccgcca aagccctgca
     3601 tctgcaaatc cgacatactt tttccttaat tggctgcgca ggctaccaga gaaaccctag
     3661 atacgcagca gaatcttttt tccaattcca ggatacccct taagacgaat ctctgtgcca
     3721 tctccggtaa ggaggacgcc atcgtaggtg ccatataccg tatgatagtc ggtgcgcagg
     3781 acaagggcgc ttagcttacg cgcactgtcc gaaatgggtg taaaggtcaa gtctaccata
     3841 ctttctgtgt cctgtattat ccaaacccct ccaacaccat atggccgggt aattttaacc
     3901 ggaggcaggg gccaggatgt cccatccaca aaaagaacat tatcgttgta ctttagatct
     3961 tcccgggtaa ctgaattacc aatttgaaac gaaacgattt ttccgtcaag gcgtcccatt
     4021 ccaatcattc ggctcatctt ggtcctcaat gaaaaatacg cctttctcac atcaaaaaaa
     4081 cccaccccgg aagatagcgg gatgcgatag tcatcatacc ccgtacctat ccatccttgc
     4141 agtgctgcgg tttgttgaaa acttgcctga caccggcggc gaaccctata tggggtaacg
     4201 gcagacagat ctgctgcgcc aggagcagac agatccatct caaacctgcc ttcgcagggc
     4261 ggacgagccc aagaacccaa aaagtcaaaa tctgcatgga ttatcttttt ttgtaaccgc
     4321 atatgaacgc gggtaaagcg actgcgagag cgacaggacg ttacactatt actaaattca
     4381 attggcattt tcataaatcc gggcggaata atccggcgat aagcaatacg ccttccggtc
     4441 gtacgattcc acaaagtcgt ttccataaaa gaaaaatacc ctgcatcaaa cagttcaatt
     4501 tcgccaatat gccgatcatc gcaaaaaaga aacctcatta tgcccataat ccggcgattg
     4561 gttatcagct ccggaattgg caaattaccg aatggtcgtt tcataccccg tatatcaaag
     4621 tggccgaatg gaccttgaaa ggtcccaaaa acaggctttc cattttcaac cggatactca
     4681 ggggccggta aaatctccct tgtatacaaa acgcacctcg tgggccgatt ataccaccgt
     4741 ttctgcttgc ggaacagcac cattaaggat atcctttttt tatgagcatc tctttgtatt
     4801 cactgggagc ggccgaagag gttaccggct ccaaacatgt cctcgagata gacgggcgac
     4861 taatactgat tgattgcggg gcctt
//
LOCUS       DAOQXQ010000031         4855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_17727_length_4855_cov_3.098125, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000031 DAOQXQ010000000
VERSION     DAOQXQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17727_length_4855_cov_3.098125"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1450
                     /locus_tag="PKH81_00950"
     CDS             149..1450
                     /locus_tag="PKH81_00950"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ96639.1"
                     /translation="MLKKVYRNELSEGMRFSAPVFFDDGENMLVSKGTPIKERELKAL
                     DRWKIQYVLTAGNPLSPDQDLDVDDPSELDELEDLDEIEEPDSFAGGPSHSAATAVAQ
                     PAVRSMHETPEIDTLEYTSEQILKLPAVLENHGLYQEYSTLIEELDQVFTNIREQKEI
                     KSRAIDKIVNDLITLIQKDRSGILGFILGGDVREMVSAKCAVNTAILSVIIAEQLSLP
                     RHRLLQVATGALLHDVGMLRIPDSIINKDGKLDDNEQQIMRSHTFYGYKFIVNELLYA
                     DEVGKAAVQHHERWDGEGYPARLAGPNIDIGGRIVSVADAFEAMVSPKAWRDSMVGYK
                     AMKNLLSDNARRFDPDIIKAMIQSMGIYPVGSIVLMNNSVIARVVDTNRDAPLRPKIR
                     VLIDEFARPYTQNEGELIDLTGNHTLFIARAIDPSEYQKKR"
     gene            1447..1818
                     /locus_tag="PKH81_00955"
     CDS             1447..1818
                     /locus_tag="PKH81_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNQ96640.1"
                     /translation="MRQETGRAGENLAASLLEQENWLIIERNWRTRRGEIDIIALDGD
                     TIVFVEVKTWPHGEEGDLEIVIGSQKVRRMVETAKCFLNTHRQYSGRYVRFDVFLVTS
                     DPLGPGPQVVRHLKDAISERV"
     gene            1815..1976
                     /locus_tag="PKH81_00960"
     CDS             1815..1976
                     /locus_tag="PKH81_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96641.1"
                     /translation="MITKRIVAEDPKIRELQEKIKDKGYIDGAIQRIALILSTKLIEV
                     KEPNNERPF"
     gene            1960..2439
                     /locus_tag="PKH81_00965"
     CDS             1960..2439
                     /locus_tag="PKH81_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96642.1"
                     /translation="MSDHSDGSHRRDRHKRHKNRDGRNTPAGSATPRQPLNPQRSGQQ
                     PRRDARQEMPPLPKLPTPVCPRCGQAIADISSAISDKTSGEPIHFDCALAFLQGAETL
                     ADGEKIVYIGQGKFAVVFFENPADTRKFKIIRVLEWEGREGRPEWRTDISSIFSQVR"
     gene            complement(2497..3768)
                     /gene="serS"
                     /locus_tag="PKH81_00970"
     CDS             complement(2497..3768)
                     /gene="serS"
                     /locus_tag="PKH81_00970"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ96643.1"
                     /translation="MLDYRFIKENLDAVKANIKARNMSADADLVVTLFDKRTALVSSL
                     QVLQTRRNDNAKAMKGKLAEEERKRLIDEGKSIKDEIASAEAELAEAEKAMEEAARQI
                     PNMAHPDAPVGKTDVENPEVKRSGEPRQFGFEPKDHVQLGESLDLIDFEAGTKVSGTK
                     YYYLKNEAVFLEQAMIMYALNILRKKGFTPFTTPDVAKEEILQGIGFNPRGNESNVYT
                     LEGEASCLVATAEITLGGYHKDEIIAREKLPLRYCGLSHCFRREAGAAGQFSKGLYRV
                     HQFTKLEMFVYCLPEDSDAIHEELRLIEEEIFAGLGIPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNGGEWGEVTSTSNCTDFQARRLNVRYKDADGKNRFVHMLNGTAISLARAI
                     IAVLENYQEADGSVTIPEALVPYCGFTEIRR"
     gene            complement(3855..4694)
                     /locus_tag="PKH81_00975"
     CDS             complement(3855..4694)
                     /locus_tag="PKH81_00975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNQ96644.1"
                     /translation="MSDYCTLAVANPFKSPVLYLPETTSTMITARKLAEEGYPSGTII
                     RTDRQTAGKGRMPLRSWESPVRESLLCTIILRMPMPTGFTLRVGLAVAQTIDTFLPSA
                     FTTKIKWPNDILSWSFNPKHADQSGKKIAGILCEAEGSFGSPHSILYAGVGINLFQTR
                     FPTSISHKATSIALLNQTPPSCDEVMERYLHFLHESLDVASWQKSISEKLVYRGETIL
                     FQAGDPEHKTDIEGIIEGIGPSGELIVSPTEQSKSKAEEVGIRCDDTGLLHLWSGEIP
                     YPE"
     gene            4770..4841
                     /locus_tag="PKH81_00980"
     tRNA            4770..4841
                     /locus_tag="PKH81_00980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4803..4805,aa:Gly,seq:ccc)
BASE COUNT         1290 a         1078 c         1215 g         1272 t
ORIGIN      
        1 ggacgagatc cttgccgata tcttaagtga acaagacatc ggaagctgtg ttcccgagga
       61 aacttggcgc gctgttgcgg ctatttttgc ctttttggaa cgcggaattc gagataaaat
      121 tgtgtaataa agtgtgtaga ggtttgtcat gcttaaaaaa gtgtatcgta acgagttgag
      181 cgaaggaatg cgtttctctg ccccggtttt ctttgatgac ggagaaaata tgctggtcag
      241 taagggaacg ccaatcaagg agagggagct aaaggcactg gaccgatgga aaatccagta
      301 tgtgcttact gcgggtaatc ctctttctcc ggatcaggat ctagatgtag atgacccttc
      361 cgagcttgac gaactggaag atttagatga aatagaagag ccagacagct ttgctggggg
      421 gccttcccat tcagccgcta ccgcggtggc tcagcctgct gttcgatcta tgcacgaaac
      481 acctgaaata gataccttag aatatacctc cgaacaaatt ttaaagcttc cggctgttct
      541 ggaaaatcac ggtctgtacc aagagtattc taccctgata gaggagttgg atcaggtatt
      601 tacgaatatt cgcgagcaaa aagagataaa aagccgggca atcgataaaa tcgttaacga
      661 cttgatcacc ctgattcaaa aagaccgttc gggaatcctc ggctttattc ttgggggaga
      721 tgtgcgggaa atggtgagtg caaaatgcgc ggtgaataca gccattttgt cggtgattat
      781 cgccgagcaa ctatcgcttc cccgacatcg attgctgcag gtagcaacag gggcccttct
      841 gcatgatgtg ggaatgttgc ggattccgga tagcattatc aacaaggatg gaaaactcga
      901 cgataatgag cagcaaatta tgcgctctca taccttttat gggtataagt ttatcgttaa
      961 tgagcttctt tatgctgatg aagtgggaaa agccgccgta caacatcatg aacggtggga
     1021 tggagaggga tatcctgccc gacttgccgg acctaatatt gatattggcg gtcgtatcgt
     1081 gtctgttgcc gatgcctttg aggcgatggt atctccaaag gcatggagag attctatggt
     1141 tggatataag gccatgaaga accttctttc ggataacgct cgccgttttg atccggatat
     1201 tattaaggca atgattcaga gcatgggaat ctatcctgtt ggatcaatcg tattaatgaa
     1261 taactcggtc attgcccgag tggtagatac aaatcgggat gcgcccttac gccccaaaat
     1321 tcgggtctta attgatgagt ttgcccggcc ctatacccaa aacgagggag agttgattga
     1381 tttgacggga aatcataccc tgtttatcgc tcgtgctata gatccttcgg agtatcaaaa
     1441 aaagaggtga ggcaggagac tgggcgcgca ggcgaaaatc ttgctgcctc tttgttggaa
     1501 caggaaaact ggctcataat tgagcgaaat tggcggacaa gacggggaga aattgatata
     1561 atagcccttg acggcgacac cattgtcttt gttgaggtta aaacctggcc ccatggagaa
     1621 gaaggggacc tggaaatcgt aatcggtagc caaaaagtca ggcgaatggt ggaaacggct
     1681 aaatgttttc ttaacacgca tcgacaatat agtggtagat atgttcggtt cgacgtcttt
     1741 ttggttacct cagatccctt gggacctgga ccacaagtcg ttcgccacct caaagatgcc
     1801 atttcggagc gtgtatgata acgaagagaa tagttgccga agacccaaaa atccgcgaat
     1861 tacaggaaaa aattaaggat aaagggtata ttgacggcgc catacagcgt atcgctctga
     1921 tattgagcac aaaactaatt gaggtgaagg aaccaaacaa tgagcgacca ttctgacgga
     1981 tctcaccgac gggatcgaca taaaagacat aaaaaccggg acggaagaaa tactcccgca
     2041 ggctcagcta cgccccgcca acccttgaac ccgcaacgta gtggacaaca gcctcgacgg
     2101 gatgcccggc aggaaatgcc ccctctgcca aaattaccaa ccccggtctg tcctcgctgt
     2161 ggacaagcga ttgcggatat atcctcggct atttccgata aaacatcggg agagcccatt
     2221 cactttgatt gtgcccttgc ttttttacag ggagcggaaa ccctcgcgga cggtgaaaag
     2281 atagtctata ttggacaggg caaattcgcc gtggtgtttt ttgaaaaccc tgcggatacc
     2341 cggaagttca agattatcag ggtgcttgag tgggaaggac gggaaggccg tcctgagtgg
     2401 agaactgata tttcatcaat ttttagccaa gttcgctaac agcgtatatt cgtaaaaaaa
     2461 aacctccggg ctttccggag gtttttttta tcattgttac cgacgaatct cggtaaagcc
     2521 acaataggga accaaggcct ccgggatagt aactgatcca tcggcctcct gatagttttc
     2581 caataccgct ataattgcac gtgctaaaga aatagcggtg ccattgagca tatgcacaaa
     2641 gcggttttta ccgtcagcat ctttgtatcg cacgttaagg cgacgcgcct gaaaatcggt
     2701 acaatttgat gttgaggtta cctctcccca ttcaccgcca tttctgccgg gcatccatgc
     2761 ttccaaatcc catttgcgat atgcaggagc gcccaggtct cccgtacagg tatcaacaac
     2821 cctaaaaggg atacccagac ctgcaaagat ttcttcttca ataagtctga gctcctcatg
     2881 tatcgcatct gaatcttcag gaaggcaata gacgaacatt tcaagtttgg taaactgatg
     2941 aacgcgatag agcccctttg agaattgacc ggcagcaccg gcttcccggc ggaagcaatg
     3001 tgacagaccg caatacctaa gaggaagttt ttccctggca atgatttcgt ccttgtgata
     3061 tccgccaagg gtaatctctg cggtagcgac aaggcaactt gcttctccct ccagggtata
     3121 tacattcgat tcgttacctc ggggattaaa cccgatgccc tgtaaaatct cttctttggc
     3181 gacatccgga gtagtaaagg gggtaaagcc ttttttgcga agaatgttaa gggcatacat
     3241 aatcatcgct tgttcaagaa acaccgcttc gtttttaagg taatagtact ttgtgccgga
     3301 aaccttggtg cccgcttcaa aatctattag atcaagggat tccccaagtt gcacatgatc
     3361 cttgggttca aaaccgaatt gtctgggttc gcctgatcgc ttgacttcgg gattttccac
     3421 gtctgtctta ccaaccgggg cgtcaggatg ggccatattt ggaatttgac gggctgcttc
     3481 ttccatagcc ttttcagctt cggcaagctc agcttctgcg ctcgcaatct catccttaat
     3541 ggatttccct tcatcaatta aacgctttcg ctcttcttcg gcaagcttgc ccttcatcgc
     3601 ttttgcatta tcattgcggc gggtttgaag aacctgcagg gaggagacaa gggcggttcg
     3661 cttgtcaaaa agggtgacca caagatccgc atcagcgctc atgttccggg ccttgatatt
     3721 cgcctttacg gcatcaagat tttctttgat aaatcggtag tcaagcataa tgacctgata
     3781 ataaaccttc ggaccgggga acttcaagtg gggaagaagt ggaaacagga aatccggggg
     3841 gggtagggca gtctttattc agggtaggga atctcgcctg accagaggtg gagtaaacca
     3901 gtatcatcac agcggatccc cacttcttcg gccttgcttt ttgactgttc tgttgggcta
     3961 acgatgagct ctcctgatgg tccgatacct tcaattattc cttcaatatc tgttttgtgt
     4021 tctgggtctc cagcttgaaa aagtattgtt tctccacggt acaccagttt ttcggaaatc
     4081 gatttttgcc aggaagctac atcgagtgat tcatgcaaaa aatgaagata tcgttccatt
     4141 acctcatcgc acgatggcgg tgtttggttg agtaaggcga ttgaagtagc cttatgggaa
     4201 atagatgtgg ggaaacgtgt ttgaaacaga tttatgccga cacctgcata gagtatggaa
     4261 tgaggtgagc caaaggatcc ttcggcttcg cacagaatac cggcgatttt ttttcctgat
     4321 tgatctgcat gctttggatt gaaagaccac gaaaggatat catttggcca tttaatcttt
     4381 gtggtaaaag cggaagggag aaaggtgtct atggtttgtg ccactgcaag ccctacccga
     4441 agcgtaaatc ccgttggcat aggcatgcgg agtatgatcg tacacaaaag gctttcccgt
     4501 acgggagact cccagcttct taagggcatg cgtcctttgc cggctgtttg ccgatctgtg
     4561 cgaatgatcg tccccgaagg atacccttct tctgcaagct ttcgcgcggt aatcattgtg
     4621 cttgttgttt ccggaagata tagaacaggg gatttaaagg ggttcgcaac agcaagtgta
     4681 caatagtcgc tcataccaca tagtagcagt acaaaaaagt gttgacaact gttcaggaaa
     4741 aaagggatac taacgtgcgt aataattttg cggatgtcgt ataacggcta ttacccgagc
     4801 ttcccaagct cgtgacgagg gttcgactcc cttcatccgc tgtaatttct cttac
//
LOCUS       DAOQXQ010000032         4751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_18245_length_4751_cov_3.420571, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000032 DAOQXQ010000000
VERSION     DAOQXQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18245_length_4751_cov_3.420571"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKH81_00985"
     CDS             <1..421
                     /locus_tag="PKH81_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNQ96645.1"
                     /translation="VTLVEKAQQTIDTQEAEKLQKKMANETFTLQDMLDQIERMSKMG
                     SLQSMLEMIPGMAGQLQNSDIDISALKVQKAIIQSMTLKERSNHLIIGPSRRKRIARG
                     SGTSVGEVNKLIKQFEKMRQTMRKFSKNKGLAGKQLF"
     gene            complement(413..1513)
                     /locus_tag="PKH81_00990"
     CDS             complement(413..1513)
                     /locus_tag="PKH81_00990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ96646.1"
                     /translation="MPISSDVLTQFNHLEKAGALDALGDLRKENRELERIITEATLLV
                     SYTDVSSMLDYVIARFLDHFIPEFLGFLVHPPRGDALRQYCYRNLKETDETIPEQYYD
                     LLKNYFERTPGRISFEQLVETFGKDILTLNPEMVFPMFGIGGLYGIVFLGKKIVGGAY
                     SELEQKYVDRIIRFLSVSMQNGLHYESSITEPKTGLFTHDYFVRRLDEAIARTKRKNT
                     GAGILMLDVDHFKRFNDSWGHVTGDLVLVTLAGVLAHIVREEDCAARFGGEEFSVLVA
                     DCSPVDLMMIAERIRNAVASIRIPAPDGSQELSVTVSIGARVIESTPGMTSLILIGDA
                     DKALYRSKNEGRNRSTLYHQGLLMRAMMKASK"
     gene            1594..2448
                     /locus_tag="PKH81_00995"
     CDS             1594..2448
                     /locus_tag="PKH81_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HNQ96647.1"
                     /translation="MNIFNNDDFVVAGFLMPHPPVIIEAVGKNRISSVSNTINACRSL
                     GALAATLDCQTIVCISPHARRFSDAIYMYDSPILSGDFGRFGSPDIGISMPLDRPFLD
                     ALEDSLAHEFLQGCPQSSLDGDPEYPAVPLDHGVLVPLSFIAPHIPTASLVAISPSVV
                     ISDAPKLARAISVAANRVQKRTLVICSGDLSHRVSPDDVSGTASEGARFDTAVADAFR
                     TGCLAELFALETTMVMRAEECGYTSLLVLASLYPDVQSSLLSYESPFGVGYCVASVTP
                     LKKGGEGP"
     gene            2445..2966
                     /gene="amrA"
                     /locus_tag="PKH81_01000"
     CDS             2445..2966
                     /gene="amrA"
                     /locus_tag="PKH81_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNQ96648.1"
                     /translation="MNTHDTPSIPVLIARATIEMFVRSGKIPSAEDLPPFGTSVAGCF
                     VSLHRQGELRGCIGTILPTKENIRAEIIRNAVSACSDDPRFDPVSPQELADLEIHVDI
                     LDPPQPVSSVQELDPHKYGVIVTSGIKRGLLLPDLAGVDSVEEQLAIACRKASISPQE
                     HYSMERFTVTRYS"
     gene            2967..3863
                     /gene="amrS"
                     /locus_tag="PKH81_01005"
     CDS             2967..3863
                     /gene="amrS"
                     /locus_tag="PKH81_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ96649.1"
                     /translation="MKEAAFCKADKDGAVLCLLCPHNCLIPVGAHGRCRVRVNRGGTL
                     YAESWGRISSLALDPIEKKPLSFFHPQSQILSVGSWGCNLSCRYCQNHRISQECVPLF
                     LEEQKSSDIYLTPQDLVATAREAVPRGNIGVAFTYNEPLVSIEYLLEAASLVQQAGLV
                     NVVVTNGFIQPDCLDAVLPYITAMNIDLKAFNDHFYQTICGASRQPVMESIVRAVKKC
                     HVEITTLIIPGCNDAPQEIEALARWLSTLSPDIPLHLTRHHPDYRMLDNKAASLDHLM
                     DLSRIASTYLSRVVLGNVSGIS"
     gene            3896..4240
                     /locus_tag="PKH81_01010"
     CDS             3896..4240
                     /locus_tag="PKH81_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNQ96650.1"
                     /translation="MQDETIISSSKIGQRLDAIVKKEQVSCLMFNNGKIPIVSVITIG
                     RSHDNDVVIDSKLASRHHAIIQKIKEDYFLKDLGSTNGTLLNGERIPQDKYLRLNRED
                     TISIGKTNLILG"
     gene            complement(4226..>4751)
                     /locus_tag="PKH81_01015"
     CDS             complement(4226..>4751)
                     /locus_tag="PKH81_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96651.1"
                     /translation="QERILEEALGQAEGIVHIEKASTTMARIYRIRAKAWSHIIKDNP
                     EGTNPLEKDILSRRCLEAWLAMRHLETASILTHVQFLHDKTTPTLERLIERANNCYDI
                     IERMRGGTLKNRANRFTKRAIIVPGQAIEMNRFRKVYLEDKKTALTEATRAIQNSFRE
                     CITEYRESVGSAKD"
BASE COUNT         1170 a         1126 c         1096 g         1359 t
ORIGIN      
        1 tgttacttta gtggaaaagg cccagcaaac catcgatacc caggaagcag aaaagcttca
       61 aaaaaagatg gcaaacgaga cctttacctt acaggatatg cttgatcaaa ttgagcggat
      121 gtccaagatg gggtccctgc agtccatgct ggaaatgatt cccggcatgg ctggccaatt
      181 acagaattcc gacattgaca tatctgcgtt aaaagtgcaa aaagcgatta tccagtccat
      241 gacgttaaaa gagcggtcta atcatttaat tattggacct tcacggagaa agcgtattgc
      301 ccgtggttcg ggaacctcgg tgggagaggt aaacaagttg ataaagcaat ttgagaagat
      361 gcgtcaaaca atgcgcaaat tttccaagaa taagggtctc gctggcaagc agttattttg
      421 atgccttcat catggcgcgc atcagcaatc cctgatgata gagggttgat ctgtttcttc
      481 cttcgttctt tgatcggtat aaggctttat cagcatcccc aattaaaatt aacgaggtca
      541 ttccgggggt gctttctatt acccgggcgc caatgcttac cgttaccgac agttcttgac
      601 ttccatcggg agcgggtatt ctaatcgaag cgacggcatt tctgattcgt tcggcgatca
      661 tcatcaagtc taccggggag cagtctgcaa ccaggacaga gaattcttct cccccaaagc
      721 gggcggcaca atcttcttct cgaacgatat gggctaaaac tccggcaagg gtaacaagga
      781 ccaggtctcc ggtaacatgc ccccaggagt cgttaaaacg cttaaaatga tcaacatcaa
      841 gcatcagtat cccggcaccg gtattttttc gctttgttct ggctatggcc tcatcaagac
      901 ggcggacaaa ataatcatgg gtaaaaaggc cggttttggg ctctgtaatc gagctttcat
      961 aatgaagacc gttttgcatg cttaccgata aaaaccggat aattcggtct acatattttt
     1021 gttccagctc tgaataggcc cccccgacga tttttttacc aagaaagaca ataccataaa
     1081 ggccccctat accaaacatg ggaaaaacca tctcgggatt aagggtcagg atatcttttc
     1141 caaaggtttc gactaattgt tcaaaggaga ttcgtccggg tgttcgttca aaataattct
     1201 tgagtaaatc ataatattgc tcagggatgg tttcatcggt ttctttaaga ttacgataac
     1261 agtattgacg tagcgcatct ccccgagggg gatgaactaa aaagcccagg aattccggaa
     1321 taaagtgatc aagaaagcgg gcaataacat agtcgagcat cgagcttaca tcggtatacg
     1381 agacaagcag cgtcgcttcg gtgataatgc gttccagttc ccggttttct tttcgcaggt
     1441 cgcccagggc atcaagtgcc ccggcctttt ccaggtgatt gaactgtgtc agtacatctg
     1501 atgaaatagg catattgcta ttgtaagcct gtttactaaa aatgtcagta aattgctttc
     1561 tttacggtaa aaacggttct atacttccat attatgaata tctttaataa cgatgacttt
     1621 gtggtcgcag gctttttaat gccacatcct ccggttatta ttgaagcggt tggtaaaaat
     1681 cgtatttcca gcgtatcaaa tactattaat gcctgtcgtt ctttgggggc ccttgccgca
     1741 accttggact gccaaaccat agtatgcatt tctccccatg cacggcgctt ttccgacgct
     1801 atatatatgt atgatagtcc gatcttatca ggagattttg gtcgttttgg ctcccctgat
     1861 ataggcattt ccatgcccct cgatcgacct tttttggatg ctctggaaga ctccctcgct
     1921 catgaatttc ttcaaggttg tccgcaatca tcccttgacg gtgatcctga atatccggct
     1981 gttcctttgg accatggtgt gcttgttcct ctttctttta tagcccccca tattcccacg
     2041 gcatctcttg ttgccatttc tcccagtgtt gttatttctg acgcgcccaa actggccaga
     2101 gctatctctg tggctgccaa ccgcgtacaa aagcgtacgc tggttatttg tagtggggat
     2161 ctctcccatc gggtatcccc ggatgatgta agtggaaccg cttctgaagg ggcacgattc
     2221 gataccgctg ttgcggatgc gtttcgcact ggctgtttag ctgaattatt tgcactggag
     2281 accacaatgg ttatgcgtgc agaagagtgt ggctacacct ctttactcgt tcttgcctcg
     2341 ctctatcctg atgtacagag tagcctcttg tcttatgaat caccctttgg ggttggctac
     2401 tgcgttgcct cggttactcc gctaaaaaaa gggggagaag gcccatgaac acacatgata
     2461 cgccatccat tccggtactc atagcacgag caacgatcga gatgtttgtt agatccggaa
     2521 aaattccttc tgcggaagac ctcccccctt ttggcacttc tgtggcaggt tgttttgtgt
     2581 cccttcacag acagggtgag cttcgcggtt gtattggaac cattttaccc acaaaagaaa
     2641 acatacgagc cgaaattatt cgcaatgcag taagcgcttg ctccgatgat cctcgctttg
     2701 atccagtatc tccccaggag cttgcggatc ttgagattca cgtagatatc cttgatcctc
     2761 cccaaccggt ctcgtccgtg caggaactcg atccgcacaa gtatggtgtt atcgttacct
     2821 cgggcattaa aagaggtctt ttattgcccg accttgcggg tgttgattca gtagaggaac
     2881 aactcgccat tgcgtgccgt aaggcatcta tttctcccca ggagcattac tccatggaaa
     2941 ggtttaccgt taccaggtac agttagatga aggaagcagc cttttgcaag gcggacaagg
     3001 atggggcggt gctctgtctt ctttgtcccc ataactgcct tattcccgtt ggcgctcacg
     3061 gtcgatgtcg tgtacgggtg aaccgtggag ggactctgta tgcggagtcc tggggtagaa
     3121 tttcttcttt ggcccttgat ccgatagaaa aaaaaccgct gtcctttttc catccacagt
     3181 cgcagattct gtctgtgggc agctggggct gtaacctcag ttgtcgctat tgtcaaaatc
     3241 atcgaatatc acaggagtgt gttccccttt ttttggaaga gcaaaagtcc tccgacatct
     3301 atctaacccc acaggatctt gtcgccacag ctcgtgaggc ggtacccaga gggaacattg
     3361 gggtagcctt tacctacaat gaacccttgg tgtcaatcga gtatcttttg gaggcggctt
     3421 cccttgttca acaagctggt cttgttaacg tggttgtaac aaatggtttt atacaacctg
     3481 attgtcttga cgcagtgctt ccgtacatta ccgcgatgaa tatagacctt aaggctttta
     3541 atgatcattt ttatcaaact atttgtggcg cttcccgcca accggtaatg gaatctatcg
     3601 tccgggcagt caaaaaatgt catgtagaaa ttactacctt gattattcca ggatgtaatg
     3661 acgctccaca agaaatagaa gcgttagctc gctggttaag cacgctttcc cctgatattc
     3721 cgctccattt aacccgtcat catcccgatt atcgcatgtt ggataataag gctgcgtccc
     3781 ttgatcattt gatggactta tcgcgaatcg cgtcgacata cttatcgcgg gtggttctgg
     3841 gcaatgtttc cggaatttct tgagtcaatt acagaagggg ggtatactga tagctatgca
     3901 agacgaaaca ataatcagtt cgagcaagat tggccaacgg cttgacgcaa tcgtcaagaa
     3961 ggaacaggtg tcgtgcctta tgttcaataa tggcaagatt cctatcgtta gtgttattac
     4021 catcggccgc tctcatgata atgacgtcgt tatagacagc aagctggcga gtagacatca
     4081 tgccattatc caaaagatca aggaagacta cttccttaaa gaccttggca gcactaacgg
     4141 gacgcttctt aacggagaac gaattcccca ggacaagtat ttgcgtctaa atagggaaga
     4201 cactatctcg atcgggaaga caaacttaat ccttggctga gccgacgctt tcgcggtatt
     4261 ccgtaataca ttcacgaaag ctgttttgaa ttgccctggt agcttctgtc agcgccgttt
     4321 ttttgtcttc aagatagact ttcctgaatc tgttcatctc aatcgcctga ccaggaacaa
     4381 taatcgctct ttttgtaaat cggttagccc tgttttttag ggtccccccc cgcattcgct
     4441 cgatgatatc gtagcaatta tttgcacgct caattagccg ctccagggtt ggtgttgtct
     4501 tgtcatgcag gaactgtaca tgagtcagta ttgacgccgt ttccagatgg cgcatggcaa
     4561 gccatgcttc caaacatcgg cgactgagta tatctttttc tagcggattg gtcccttcgg
     4621 gattgtcctt gataatatgc gaccacgcct tggcgcgaat gcgatatatg cgcgccatgg
     4681 tggtggatgc tttttcaatg tgtacaattc cttcggcttg tcctagcgct tcttccaata
     4741 tgcgctcttg a
//
LOCUS       DAOQXQ010000033         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_18264_length_4748_cov_3.358406, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000033 DAOQXQ010000000
VERSION     DAOQXQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_4748_cov_3.358406"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="PKH81_01020"
     CDS             complement(<1..1423)
                     /locus_tag="PKH81_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ96652.1"
                     /translation="MNHKKKDSLIIPLCPHLLFCIIIFLITACQSTPKEKLYPGTPSL
                     LSGVHPEMNTPGFWIARHGDPDGRILDAQGIDRINGEVQAQGLTRDLGMIQQVTSEDL
                     AKDFIKTITWVRKVSLFTKNGKRLHKDFTAACENEMNIQNLPPLISPRYGYTVRQTNV
                     RILPTDEPGYEEKGSEFIDHIQASSIDWGTPLVLLHQSKSGEWLFVHTELVSGWIRTA
                     EVAHIEADAFFTLYRRSEPLVVTAAKADLWADTSKTRFLGSVRMGSYLFAPIAISEAA
                     SASEGLLEIRMSARSKNGTFIEIPAWVESKDVSKGFLPYTARTIYEQSFKLLHSPYGW
                     GGSFGERDCSQFLCEIFRTVGIVLPRNSSRQARVGIPIEWIERQTSTDAKKDILTNGA
                     IGGATLLRFPGHIMLYLGSVYGEPYAIHSTWGYREKIGRSNVTRLVNRVVVSNLNLGD
                     GSDKGSHIERLTNAVIIAATEDFY"
     gene            1481..2197
                     /locus_tag="PKH81_01025"
     CDS             1481..2197
                     /locus_tag="PKH81_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNQ96653.1"
                     /translation="MNNHIRCNRMLLAFLFGVALVATSACAQLPSRDESVPPMLATKR
                     HPVQSEARKILMKEYSLLHYGLDQWTLQNPQMIVIHYTGTDSDEYSLSVFTSDTLASS
                     RTEISSGGAVNVGVHYVITRDGAIWSLLPETDMGRHIIGYNHTAIGIEMTGSSGDVLT
                     DEQLASCAALVANIASRNSGIRYLVGHHEYVQKGRSHLYLYKELVDDYTPTVKHDPGA
                     CFMYLLRETLDREYSVLLED"
     gene            2243..3757
                     /locus_tag="PKH81_01030"
     CDS             2243..3757
                     /locus_tag="PKH81_01030"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNQ96654.1"
                     /translation="MKLKLILVTVLCVLYFASCGKSLPDGELVIENSKNFKIRVNGRT
                     REGTFYIPLTYDETRSYSLVFALHGASHTGAEFQKTTLFDPMAEEHDVIMVYPDGIGR
                     RWDSAEDTALFEAMIKAFSGKYSIDSSRIYLAGFSAGAIKAYELAAVLPGRFAAIAPV
                     AGAMRADADRAALAPTAVLHIHGKRDEEVPFVGLPEWKLLSAPESVELWKGVNGAMGE
                     GEEFFRSRTAIGLAWEGQGHTVAQIFDDDCAHYWPPYASELILDFFYNNPARQGRLVI
                     RRDGLPLTSAIGTVIPLRADITQSESTSPVEEVTWYTNGSQAAVSKKAPWESEWSVNL
                     GGVRRLTAVARLADGTELRSTRNPFLLVSSSLSKDADTAGAETLLTVVDAFSTKIEDS
                     TLEAKNAVDGDIYTRWGSEWTDNESLSLDLGSPCTVTALTLYWEMAYGKEYCIETSMD
                     KESWTIVAQKNDGLGSIEFLLFPATQARYVRIRGIRRATEWGYSLFEVFVYGKK"
     gene            3760..3912
                     /locus_tag="PKH81_01035"
     CDS             3760..3912
                     /locus_tag="PKH81_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96655.1"
                     /translation="MRELPFLYRSILDTATITALVMIAFFAVMDRMGIEKGRRDPLMM
                     HFAFFP"
     gene            3909..4733
                     /locus_tag="PKH81_01040"
     CDS             3909..4733
                     /locus_tag="PKH81_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ96656.1"
                     /translation="MSLSGYVFFDNAFAILLFYEPIRSRICTVGVSVCAFALMVSYSG
                     LITQIFDLPRRNRPWFRACIALASVSLLFSMTVYLHGWEWYMMYFDGPAITCYIIIFV
                     IIETRMAISLRSGKGRYRNAGILLVSLHVMILSLFFWRVLININTANYFFNNSILLVS
                     MSFLFPVVISLHRSGAYRELQELRMKQESQARRAQKGLRDLFGVHNEGKIKLTDREIE
                     VCEALLEGLEYKEIQDRTGLSLSGVKKRVHLLYQKIGVQNRTELSNLIQKMRSSAE"
BASE COUNT         1127 a         1091 c         1188 g         1342 t
ORIGIN      
        1 cgtaaaagtc ctccgtcgct gcgatgatca ccgcattggt aagccgctca atgtgagaac
       61 ccttatcgct tccatctccg agatttaggt tagacacaac tactcggttt acaagacggg
      121 ttacgttgct tcgtcctatt ttctctcggt atccccaggt ggaatggatt gcgtatggct
      181 ctccgtaaac cgatcccaga tagagcatga tatgaccggg gaagcgaaga agtgtagctc
      241 ctccgatcgc tccattagtt aggatgtctt tcttggcgtc tgtcgatgtc tgtcgctcta
      301 tccattcaat cggaatgcca acccgtgcct gccgagatga gttacgcggc aagacgatgc
      361 cgacagtacg gaatatctcg cagagaaatt gtgagcaatc acgttcccca aagctgccgc
      421 cccatccata gggagaatgg agaagcttga aggattgttc gtatatcgtg cgggcagtat
      481 agggaaggaa tcctttagaa acatccttgc tttcaaccca ggcgggtatt tcgatgaacg
      541 ttccgttttt gcttcgcgcg ctcatgcgta tttctagaag tccttcagag gcgctagctg
      601 cctcagaaat cgcaattgga gcaaataggt aacttcccat tcgtactgaa ccgagaaagc
      661 gcgttttact tgtgtctgcc cagaggtcgg cttttgcggc ggtcacaacg agtggttcgc
      721 ttctgcggta cagggtaaaa aaagcatcgg cttcgatgtg cgccacttct gcagtgcgta
      781 tccatcccga tactaattcc gtatggacaa aaagccattc gccgctttta ctttgatgca
      841 acagaactaa cggagtcccc cagtcgatac tgcttgcctg aatatgatcg ataaactcgc
      901 tccccttctc ttcatagcct ggctcgtccg tgggcaggat acggacattc gtttgcctga
      961 cggtgtatcc ataccgggga gatataagag gtggaaggtt ctgaatattc atttcgtttt
     1021 cgcacgcggc tgtgaaatct ttatggagtc gctttccgtt tttggtaaag agggatactt
     1081 tgcgcaccca ggtaattgtc ttgataaagt ctttggcgag atcttctgaa gtcacctgtt
     1141 gtatcattcc caagtcgcgg gtaaggcctt gcgcttgaac ttccccgttt atcctgtcga
     1201 ttccctgcgc gtcaaggata cgtccgtcgg ggtcgccgtg tcgggcaatc cagaatccgg
     1261 gggtgttcat ttccggatgc acaccagaga gaagggaagg agtcccgggg tagagctttt
     1321 cctttggcgt actttggcac gcggtaatca aaaagatgat tatacagaat aaaaggtgcg
     1381 ggcacagagg tattataagg gaatcttttt tcttatgatt cattgtagac agtgttagcc
     1441 agtagcgaca aaaggtcaag aggatgtata gtgggactat atgaataatc atatccgatg
     1501 taaccgaatg ctcttggcct ttttatttgg ggtcgctctc gtagcaactt ccgcttgtgc
     1561 ccagcttcct tccagagacg agtctgtacc gccaatgctc gcgactaaaa gacatcctgt
     1621 tcaaagcgag gcgcgaaaga tcttgatgaa ggagtattcg ctcctccatt atggacttga
     1681 ccaatggacg ctgcaaaatc cgcagatgat tgtaattcat tataccggaa ccgactccga
     1741 tgagtattct ctttctgtgt ttacgagcga tacccttgcg tcatcccgta ccgagatttc
     1801 ttctgggggt gcagttaatg ttggggttca ctatgttatc acccgggacg gggcaatatg
     1861 gtccttgctt ccggaaactg atatgggccg acacataata ggatacaatc atactgcaat
     1921 cggtatagaa atgactggct cctccgggga tgtattgacc gatgaacagc ttgcctcttg
     1981 tgccgccctt gtagccaata ttgcttcccg taattccggt atccgctatc tggttggaca
     2041 ccatgagtat gtccaaaagg gtcgatctca tctctaccta tataaggaat tagttgacga
     2101 ctacactccg acggtaaaac atgatccggg agcttgtttt atgtatcttc ttcgcgaaac
     2161 acttgatcgg gagtattctg tattgttgga agattaaaaa aaagagatgg acagactgaa
     2221 tagtttttta tagcatactg gtatgaaatt gaaacttatt ttagtaacag tgctgtgtgt
     2281 gttgtatttt gcttcctgtg gaaaatcgct tccagacggt gagctcgtca tagaaaatag
     2341 caaaaacttt aagataaggg ttaacggccg tacccgtgaa ggaacctttt atatccccct
     2401 gacctacgat gaaactcgtt cgtattctct ggtttttgcc ctgcatgggg cttctcacac
     2461 cggagctgag tttcagaaaa ccacgctctt tgatccaatg gccgaagagc atgatgtaat
     2521 aatggtctat cccgatggca ttgggcgcag gtgggactcc gccgaggata ccgcattatt
     2581 tgaagcgatg ataaaggctt tttccggaaa atattctatc gactcttctc ggatatatct
     2641 tgcgggattt tctgccgggg ctataaaggc gtacgagctt gcggccgttc ttcccggtcg
     2701 tttcgccgcg atcgcgccgg ttgccggagc gatgcgggcc gatgcagata gagcagctct
     2761 tgctcccacc gcggtcttgc atatccatgg aaagcgggat gaagaagtgc cctttgtagg
     2821 tcttcctgaa tggaagctcc tgtcggctcc cgagtctgta gaattatgga agggagtaaa
     2881 cggcgccatg ggagaggggg aagagttttt ccggtcccgc accgccatcg gtctagcttg
     2941 ggagggtcaa gggcatactg ttgcccagat ttttgacgac gactgtgctc attactggcc
     3001 cccgtatgca tcggaattga tccttgattt cttctataat aatcctgccc gtcaaggtcg
     3061 gcttgttatc cgccgcgacg gccttccgct tacctctgct attggaacgg tgataccgct
     3121 tcgcgctgat atcacacaga gcgaaagtac ctccccggtt gaagaagtca cctggtacac
     3181 aaacgggagc caagccgctg tttctaaaaa ggctccttgg gaaagcgaat ggtcagtaaa
     3241 tcttggtggc gtcaggcgcc ttactgcggt tgctcgcctt gcagatggaa ccgagcttcg
     3301 atctacgcgg aatccttttc tgcttgtaag ctcatccctt tctaaagatg cagacaccgc
     3361 gggtgcagaa acacttctaa cggttgtgga tgccttttcc acaaaaattg aggatagcac
     3421 ccttgaggca aagaatgcag tcgatggaga catctatacc cgttggggca gcgaatggac
     3481 cgacaatgaa agtctctcgc tcgatctggg ttctccttgt accgtgaccg cccttaccct
     3541 ctattgggaa atggcgtatg gcaaggaata ttgcatagag acttcgatgg ataaggaaag
     3601 ctggacgatc gtggcacaaa aaaatgatgg actggggtct atcgagtttt tgttatttcc
     3661 agcgacacag gcacgatacg ttcgcattcg cggtatccgc cgcgcaactg aatggggcta
     3721 ttcacttttt gaagtgttcg tttatggtaa gaagtaatcg tgcgagaact accctttctt
     3781 tacagatcta tccttgatac cgcgacaatt actgcgcttg ttatgattgc attttttgca
     3841 gttatggacc gcatgggaat agaaaaagga cgtcgcgatc ctcttatgat gcacttcgct
     3901 ttttttcctt gagtctctcc ggctatgtat tctttgataa tgcctttgcg atattgcttt
     3961 tttatgaacc gattcgctct cgtatttgca ctgtgggggt ttcagtctgt gcctttgctc
     4021 ttatggtttc ctattcgggc ttgataaccc agatttttga cttgccccgc cgtaaccgtc
     4081 cctggttcag agcctgtatc gctctggcga gtgtttccct gctgttcagt atgacggttt
     4141 atttacacgg atgggaatgg tacatgatgt attttgatgg gccggctatt acctgctaca
     4201 tcattatctt tgtgattatt gaaaccagaa tggctatctc tctgcgatct ggtaagggga
     4261 gatatcgtaa tgccggtatt ttgcttgtct ctcttcatgt catgatcctg tcactctttt
     4321 tttggcgtgt acttatcaat atcaatactg cgaactactt ctttaataat tccattcttt
     4381 tagtctcaat gtcgtttttg ttccccgtag ttatttcact gcatcgcagc ggggcgtacc
     4441 gggaacttca ggaattaagg atgaaacaag aatcgcaggc acgcagagca cagaaaggtc
     4501 ttcgtgattt gtttggagtg cataatgaag gtaagatcaa attgacagac cgcgagattg
     4561 aagtctgtga agcgcttctt gaaggtttgg agtacaagga aatacaggat agaaccggac
     4621 tctctttaag cggagttaaa aagagggttc accttctgta tcaaaaaatt ggcgtgcaaa
     4681 accgaactga actgagtaac ctgatacaaa aaatgcgctc tagtgcagaa taggccctgc
     4741 agcaaacg
//
LOCUS       DAOQXQ010000034         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_18515_length_4701_cov_2.859019, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000034 DAOQXQ010000000
VERSION     DAOQXQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18515_length_4701_cov_2.859019"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="PKH81_01045"
     CDS             complement(<1..1903)
                     /locus_tag="PKH81_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ96657.1"
                     /translation="MKSTIRLGLQINSTDSDYAKGLIFGVQAWCQEQNVPLIVFSGRS
                     FGWPYGYEYQNSAIYSHLHTGNLDALIIISGTQCNFISRDDFTRFIENLAPLPLISVT
                     IPVEGIPSVLVDNKSGLRNLLNHLVDEHDCSRIAVMKGPDDNDEACQRFSVYQEVLKA
                     RGLPVDPDLVFYGDFSAEYSLVSLRQHVDARGVDFDALVCLNDTMAIGCLHYLREKGF
                     RVPEDVIVTGFDDILRARYDVPTLTTVSQDLIEQGKIAAEYAWRRAKGEDDIPPVTVH
                     RTRAIFRQSCGCVPREPASFIAISEEGAKIPANPLASFETGMEWFRIQDDIVRLRHYL
                     SHLISLLSLQDLMEDLRTSLESFMIRSCVIVLYRQEIRTRRSDVFVLPTEAEVLLCYD
                     EETPRTGRKKPVLFNPREHLYPPELFSGRRRNLVACALYHREEQLGYILYEPGNCDPS
                     IYETLCVQLSTTIRSALAFAEKQAAEERLNDALYNLEKSNRKLSTISRTDELTGLYNR
                     RGFVTLGQQTIDLALRMGKRGLVVFGDMDGLKLINDSLGHEAGDRAIIAMGQALKKVF
                     RNLDIVSRLGGDEFAVVAVDIDTSFVNTLRNRLERVLTLYNEISGEEYILSLSLGAVE
                     FSGDKNNNLE"
     gene            complement(1907..4015)
                     /locus_tag="PKH81_01050"
     CDS             complement(1907..4015)
                     /locus_tag="PKH81_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HNQ96658.1"
                     /translation="MSVLAKDNDFYSLHGIWGADAALALGRIGRGSGMVVGDVQPPRR
                     GMAVGYKVGSSDPVFLPFIPNIRVGLGADAYQAEEASADAAAHPEVLPGGIALKFFGS
                     TDIERRLSMSGESWTAGKMTLEVVSFFGSVPDPASASREILREKMCPAIYARVCFDNR
                     ESDEPMIGVFGMQGVRRPLSDTPALGLLGFAHRGEWGFAARPEEGVSEVMDWMSLAAC
                     FDPGRRVLRHLANEGCLKFNVPPRQKKEYLVALGAFRDGIVTQGLKTRAFQNTLFTDL
                     DDILVHALKVASSRLAEAHRLDVELDSRSLSDDRKFLICHAAHSYNASTELLVDESGS
                     PIFIVNEGEYQMMNTLDLTVDQVFHELYYSPWTVKNELDFFFSRSSYRDSIGLAFCHD
                     QGVSDCFTSQGTSSYELPWLTGCFSFMSYEETLNWILCACLYTHNADDGSWLRAHMES
                     FEDAVTSIISRDRNGDGVMDVDSDRCDGGAEITTYDSLDVSLGQARNNLYVAVKAWGA
                     LVSAAAVLQAKANDQKAMSLADKALSCAALIAKTVVSRVDPQQGYIPAVFEGGNTSKI
                     IPAVEALVYPYFSGVPDAVRVDGPYGELISALKKHLDIVLVPGVCLDPVSGGWKLSST
                     SRNTWPSKIFISQFVAEEILGMKDGRTTRDAAHVAWQCNGCADYAATDQLDSATGKDM
                     GSRLYPRLVSSILWQHPPIQ"
     gene            4180..>4701
                     /locus_tag="PKH81_01055"
     CDS             4180..>4701
                     /locus_tag="PKH81_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ96659.1"
                     /translation="MTKAATEQTNKTRILEAAAAEFAERGFDGARVDDIAKRAGINKA
                     LIYYYYKSKEELLVLLFQDTSSDICALLDSPKIKELDFLKPEGATLMINMFLDVLESR
                     QNVIRVVLMEIAKRSPINTHIFTLLQSIMEKMFTLSNSQDFPILENRQTAMITEFFTG
                     IMPILGYVAYHEIW"
BASE COUNT         1398 a         1203 c          958 g         1142 t
ORIGIN      
        1 tttcaaggtt gttgttttta tctccgctaa attcaaccgc gcctaaacta agagaaagga
       61 tatattcctc tccagaaatt tcattataca gagtaagaac tcgctccaga cgattgcgaa
      121 gcgtattaac aaacgaggta tctatatcta ccgcaaccac agcgaattca tctccaccca
      181 agcgtgatac aatgtccaga ttacgaaaaa cctttttaag ggcttgcccc attgcgataa
      241 tggcacgatc ccctgcttca tgacctaaag aatcatttat cagcttaagt ccatccatgt
      301 ccccaaaaac caccaagccc cgctttccca ttctaagggc aagatcgatg gtttgctggc
      361 caagagtaac aaaaccgcgc ctattataaa gcccggttaa ttcatctgtt ctggatatag
      421 tactcagctt cctgttcgat ttttctaaat tatatagagc atcattaagc cgctcttccg
      481 ccgcttgttt ttcggcaaag gcaagcgccg aacgaatagt agtggaaagt tgaacacaga
      541 gggtttcata tatcgagggg tcgcaattac ccggctcata gagtatatag ccaagctgct
      601 cctcgcgatg atacagggca caggcaacca ggtttcgcct acgtccagaa aacagctccg
      661 gaggatagag atgctctcgg ggattaaaca aaaccggttt cttccttcct gttcgaggag
      721 tttcctcgtc gtaacacaac agaacctcag cctctgtcgg aagaacaaaa acatcggatc
      781 ggcgagtcct aatttcctgc cgatataaca cgattacgca agagcgaatc ataaaagact
      841 caagacttgt tcttaaatcc tccatcaaat cctgaagcga tagaagcgat ataagatggg
      901 aaagatagtg ccgaagccgt acaatatcgt cctgtatcct gaaccattcc attcctgtct
      961 caaaagacgc taatggattc gccggaattt ttgctccttc ttctgaaata gcgataaagg
     1021 aggccggctc gcggggaaca cagccgcaac tctggcggaa aattgcacgc gtgcgatgaa
     1081 cagttaccgg aggtatatca tcctctccct ttgctcgacg ccaggcatat tcggctgcga
     1141 ttttcccctg ctcaatcaag tcctggctta cagttgtaag ggtaggaaca tcataacgag
     1201 cacgaagaat atcatcgaat ccggtaacaa ttacatcctc gggaacacga aaaccttttt
     1261 cccgtaagta gtgtagacag cctatcgcca tggtatcgtt aaggcatacc agggcatcaa
     1321 aatcaacacc cctggcgtcc acatgctgac gcagagagac caaactatac tctgcggaaa
     1381 aatccccata aaaaacaaga tccgggtcca cgggaaggcc acgcgctttc aaaacttcct
     1441 gatatacact aaacctctga caagcctcgt cattatcatc aggacccttc attaccgcta
     1501 tccgggaaca gtcgtgttca tctactaaat ggtttaataa attacgtaaa ccggatttat
     1561 tgtctaccaa tacgctcggg attccctcta cgggaatagt aacgcttatc aagggtagcg
     1621 gggccagatt ttcgatgaac cgggtaaagt catcccgtga tataaagttg cactgtgtcc
     1681 ccgaaatgat tattaaagca tccagattac cggtatgcag gtggctatat attgccgagt
     1741 tttgatactc gtacccatac ggccacccga acgaccttcc ggaaaagaca attaacggta
     1801 cgttctgttc ttggcaccag gcctgaaccc caaatattaa ccctttcgca taatctgagt
     1861 cagttgaatt gatctgtagc ccaaggcgta tcgtagattt catatcctat tgtatcggcg
     1921 gatgttgcca caatattgag cttaccaaac gtggatataa gcgagacccc atatccttac
     1981 ccgtcgcaga atccagttga tctgtggctg cataatcagc gcagccatta cactgccagg
     2041 caacgtgagc ggcatcacgc gtagtccgtc cgtccttcat ccctaaaatc tcctcggcaa
     2101 caaattggct aataaaaatc ttggacggcc atgtattacg gctagttgaa gaaagtttcc
     2161 atcccccgga aacaggatca aggcacacgc cgggaaccag aacgatatcc aggtgctttt
     2221 taagagcgga tatcaactcc ccgtaagggc cgtccacacg gacagcatca ggtactcccg
     2281 aaaaataagg atacacaaga gcttcaacag cagggattat ctttgaggtg ttccctccct
     2341 caaaaacagc cgggatgtac ccttgctgtg gatctacccg ggaaacaacc gttttagcaa
     2401 taagagccgc acaagaaaga gccttgtcag caagactcat agctttttga tcgttggcct
     2461 tcgcctgtaa taccgcggct gcggacacaa gggctcccca cgcttttacg gccacatata
     2521 aattgtttcg cgcctggcca aggcttacat caagcgagtc atacgtggtt atctcggctc
     2581 caccgtcaca ccgatcggaa tccacatcca taaccccgtc tccattgcga tcgcgagaaa
     2641 ttatagaggt tacagcatcc tcaaaagatt ccatatgtgc cctaagccag gaaccatcgt
     2701 ctgcgttatg agtatacaag cacgcacaga gaatccaatt gagcgtttct tcataactca
     2761 taaaactaaa acatccggtt aaccagggta gttcatacga tgatgtacct tgtgaggtaa
     2821 agcaatcaga aacaccctga tcatggcaaa aggcaaggcc tattgagtcc cgatacgatg
     2881 aacgtgaaaa gaagaaatcc aattcattct tgactgtcca gggagaataa tataattcgt
     2941 gaaaaacctg atctacggtt aaatccaggg tattcatcat ttgatactca ccctcgttta
     3001 cgataaatat gggagacccc gattcatcca ccagaagttc agtacttgca ttatagctat
     3061 gtgccgcgtg gcaaataaga aactttctgt cgtcggataa agagcgactg tcaagttcca
     3121 catccaatcg atgtgcctca gcaagacggg aagaagcaac cttcaaagca tgcacaagaa
     3181 tgtcgtccaa atcggtaaat agcgtatttt gaaaggctcg ggttttaagc ccctgtgtca
     3241 caattccatc cctgaatgcc ccaagagcaa ccagatattc ttttttctgc ctgggaggaa
     3301 cgttaaattt taaacaacct tcgttcgcta aatgacgtaa tacccgtcgc cctggatcaa
     3361 aacacgcagc cagggacatc cagtccatta cctcgctaac accttcctcc gggcgggcag
     3421 caaagcccca ttcgcctcga tgtgcaaatc cgagtaatcc aagagcaggg gtgtcggata
     3481 aaggacgcct aacgccttgc atcccgaaaa cgccaatcat tggctcgtcc gattctctgt
     3541 tatcaaaaca aacgcgagca tatatagcag gacacatctt ctcccgaagg atctcccgtg
     3601 aagcagaagc aggatccggc acagatccaa aaaaagatac tacctcaaga gtcatctttc
     3661 ccgctgtcca tgactcgccg ctcatcgaca atcttctttc tatatcagta gaaccaaaaa
     3721 acttaagagc gatccctcca ggaagcactt ccggatgcgc tgcagcatcg gctgatgcct
     3781 cttctgcttg gtacgcatct gcacccagac ctacccgaat atttgggata aagggtaaaa
     3841 aaacaggatc cgacgatccg accttataac caacagccat tccacgccga gggggctgca
     3901 catcgccgac gaccataccg gaaccacgac caatacgacc aagcgcaagg gccgcatcag
     3961 caccccaaat accatgcaga gaataaaaat cattgtcttt tgccaaaacg gacattacaa
     4021 gcctcctgga gtatcatctt ctgtcatagt caatcaaaaa ccgtcgattt gcaaggctga
     4081 ttctagttat ttttctttgt aagaattacc gattacaatc ttgacaaaca taacttttgg
     4141 atttattgtt aactaactgg ttaattatat ggagttcaca tgaccaaggc cgcaaccgaa
     4201 caaacaaaca aaactcgcat cctagaagcc gctgcggcag aattcgccga acgtggattt
     4261 gatggtgctc gagtggacga catagctaaa agagcgggta ttaataaagc actcatctac
     4321 tactactaca aaagtaagga agaattgctt gtgctactat tccaggatac atctagcgat
     4381 atttgtgcac tactggattc accaaaaatt aaagagcttg attttcttaa accagaaggc
     4441 gcgactctta tgataaatat gtttctcgat gtcctagaat ctcgtcaaaa tgttattcgt
     4501 gttgtcctga tggaaatcgc aaaacgaagc ccaataaaca cccatatttt caccttatta
     4561 caatccatca tggaaaaaat gttcacctta tcaaattccc aggattttcc gatcctggaa
     4621 aaccgacaaa ccgccatgat aaccgagttt tttactggaa tcatgccaat tttagggtat
     4681 gtggcttatc acgaaatatg g
//
LOCUS       DAOQXQ010000035         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_18565_length_4692_cov_2.748544, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000035 DAOQXQ010000000
VERSION     DAOQXQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_4692_cov_2.748544"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKH81_01060"
     CDS             <1..465
                     /locus_tag="PKH81_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ96660.1"
                     /translation="SLRKALHMEPDNPVLWFNLALSCRALGQRDEARNALLQAARHNP
                     GDVDILDTLAVVLHELGEDSAAEDYYQVALDIAPGNGRVWNNYGVLQFSQARYDEACK
                     SFEKSVTLVPDFEDALYNLRDTYEELGKTSEMEKCAALLERRSFPHRGVSPS"
     gene            484..1311
                     /locus_tag="PKH81_01065"
     CDS             484..1311
                     /locus_tag="PKH81_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNQ96661.1"
                     /translation="MGLKILYIAEIVGKAGVWCVKNALVDLKKAEQPDIVIANADGVT
                     GSYGLGRQHAGYLRKLGIQLAIGGDCSYYKKDLVEAIDTLSGVIRPYNYPPESPGRGW
                     RFVQAGEQKVAVVSLLGRAGFSRVHAENPFTSLGPLVERLHRETPYVIIDFHAGATAE
                     KQAFFFHADGKVSAVIGSHSRVQTSDEGILPGGTAVITDAGRTGSLDSVGGTDPVSRI
                     REYITGVPDWSKDCWARPGIQGVVIELDSSGKALTIRRFSHECEGAPESGDAMEQGG"
     gene            1313..1711
                     /locus_tag="PKH81_01070"
     CDS             1313..1711
                     /locus_tag="PKH81_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNQ96662.1"
                     /translation="MQERARVLKINGDCVDVVPLDIEVCLGCSNSECRTHGSVFTARN
                     RKKLNLSVGTHVRIGASPVTQALQAVLSIGAPIVIAVTVWWSITVCIPELADSWRVGF
                     TLLGLVSGAVACLVITGKWKAELPEVLEIL"
     gene            complement(1720..2940)
                     /locus_tag="PKH81_01075"
     CDS             complement(1720..2940)
                     /locus_tag="PKH81_01075"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNQ96663.1"
                     /translation="MDAISVLIVDDSALMRSLIGKIIENSEGLTVADKAMNGRFALQK
                     LLRAQADIIVLDIEMPEMNGIEFLKERKRLGIDIPVVILSSIAKEGARVTMECLELGA
                     SDFVTKPSGSESANLHTVANQLVELLVAYGNQYKLKKIAGTARKLPEAELRKWEARSG
                     LSELDTDRHNEKEHAQAIDLLSRAPLSLSPRRQETASAQRIVTERPKPLRESAPIEII
                     AIGISTGGPNALREVFAHLDPALPQPILVVQHMPAGFTEEFANSLNKICPLEVKEAAE
                     GDLVKPGRILIAPGDKHMEVERKPLATIVHLNDNPPRNGHRPSVDVLFASVRKEFQNR
                     SLGIIMTGMGRDGAAEIVQLYREGSRTIGQDEASSIVYGMPRVAWELGGVMEQVSLTK
                     MAETINRWGKDYSR"
     gene            complement(2951..3766)
                     /locus_tag="PKH81_01080"
     CDS             complement(2951..3766)
                     /locus_tag="PKH81_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNQ96664.1"
                     /translation="MAAFLSYADFELYRKLIYDESGITFSTTNRSILESRLRERLREK
                     QIPEVGDYYSILKKDKEELKLMLDSVTTNLTRFFRNQPHFDAMEHFIIPEVIKLKKAK
                     NDTKIRVWSAGCSTGEEPYTLAMLLKDKLPSPFTAEITASDISLKSLMVGKSGFYPEA
                     RVVGIPEPYLHRFFEHKNSGYQAKDEIMKMIRFDYHNLINDSGQRNIDILFCRNVLIY
                     FDEAAQKAVIDRFWDAMSSKSFLFIGHSESLFGMKTQFEFLKTDWACLYQKNS"
     gene            3883..4443
                     /locus_tag="PKH81_01085"
     CDS             3883..4443
                     /locus_tag="PKH81_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96665.1"
                     /translation="MSRGITACVLFFLFSLLLSCSFNYTEGDGAGKVLPEMVIEAARA
                     TRYEDARIKVVFNADVLEIYDSDRVWAGEKVSFVEYAADASGAVEMEGAASLLVIDDK
                     EGVYSLGNGAFFHLVDDDIKLTAEDLKWTKKTHQLSGSAEGLVKMEESDGSIIQGTGF
                     FADSLRRYYLFDSTVSGFLMRDGSSE"
     gene            4440..>4692
                     /locus_tag="PKH81_01090"
     CDS             4440..>4692
                     /locus_tag="PKH81_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transporter LptA"
                     /protein_id="HNQ96666.1"
                     /translation="MIARSVLMCVCVIALLTSPLAAESKKIAFNAEKMSGTSGKKDSI
                     TSLEGNASVTVGTLTIKAERIELSGKDFRYVKADGSVKGS"
BASE COUNT         1082 a         1062 c         1263 g         1285 t
ORIGIN      
        1 tctttgcgta aagcccttca tatggagccc gataaccccg tgttatggtt taacctcgcc
       61 ttaagttgcc gtgcccttgg gcagcgggat gaagcgagaa atgccctcct gcaggcggct
      121 cggcataatc ccggagatgt tgatattctt gatacccttg cggttgtcct gcatgaattg
      181 ggagaggatt ctgcagccga ggattattat caggttgctc ttgatatagc tcccgggaat
      241 ggacgggtat ggaataacta tggcgtgctt cagttcagtc aagctcgata tgatgaagcg
      301 tgcaaatcgt ttgaaaagtc cgtaaccctg gttcccgatt ttgaagatgc cctgtataat
      361 cttcgagata catacgagga gctcggaaaa acgagcgaga tggaaaagtg tgcggctctt
      421 ttggagcgac gatcttttcc acacaggggc gtcagccctt cgtaaggata tccgggagaa
      481 tgcatgggcc ttaaaatcct ctacatcgcg gaaattgtcg gtaaggccgg cgtatggtgt
      541 gttaaaaacg cgcttgtgga tctaaaaaaa gctgaacagc cagatattgt tatcgcgaat
      601 gccgacggag ttaccggcag ttacggcttg ggccgtcaac acgccgggta tttgcgtaag
      661 cttggaattc aactggcgat cggcggtgac tgctcctatt ataaaaaaga cctggtagaa
      721 gccattgata ccctctctgg cgttatacgt ccctataatt acccgccgga aagccctggc
      781 cggggatggc ggtttgtgca agccggggaa cagaaggtgg ctgttgtgtc ccttttgggc
      841 agggcaggct tttctcgggt acatgccgaa aaccctttta cctcgcttgg ccctttggtt
      901 gagcgactcc accgtgaaac tccctatgtc atcattgatt ttcatgcagg ggctacggcc
      961 gaaaagcagg cctttttttt ccatgcggat ggcaaggttt cggcggtcat aggctcgcat
     1021 tcacgggtac aaacctcgga cgagggaata cttcccggag gaaccgccgt tattaccgat
     1081 gcgggtcgta cgggtagtct ggattctgta ggcgggacgg atcccgtttc ccgtatacgg
     1141 gagtatataa ccggggtccc tgactggtcc aaggattgtt gggcgcgacc gggaatacag
     1201 ggcgtagtta ttgagcttga ctcgtccgga aaggctttga caatacgacg attttctcat
     1261 gagtgcgagg gcgctccgga atcaggtgat gctatggaac agggaggctg aggtgcaaga
     1321 aagagcgcgg gtgttaaaaa taaacggaga ttgtgtagat gttgttccac tcgatataga
     1381 ggtgtgtttg gggtgcagta acagcgagtg ccgtacacac ggcagtgtct ttactgccag
     1441 aaatcgcaaa aaattgaatc tgagtgtggg aacccatgtg cgaatcggcg cttcgccagt
     1501 aactcaggcc ctccaggcgg tgctatcgat tggcgctcca atagtaattg cggttacggt
     1561 ctggtggagt attaccgtgt gtatccctga gctggccgat tcatggcgcg tcggttttac
     1621 tttacttggc cttgtctccg gggctgtcgc atgccttgtc ataaccggaa aatggaaggc
     1681 ggaattaccc gaggttcttg agattttgta agaaggtctc tatcgtgagt agtctttgcc
     1741 ccatcggtta atggtttctg ccatcttggt gagcgagact tgctccatca cccctccgag
     1801 ttcccatgcg acgcggggca ttccgtatac gatactcgat gcctcatcct gacctatggt
     1861 tcgggaaccc tctcgataga gttgaacaat ctctgcggcc ccatcgcgcc ccataccggt
     1921 catgatgata ccaagagagc ggttttggaa ttctttgcga actgaggcaa acaagacgtc
     1981 aacagagggg cggtgcccat tcctgggcgg gttatcgtta agatgcacaa tggttgcaag
     2041 cggtttgcgt tcaacttcca tgtgtttgtc ccccggcgca atcaggattc gcccgggttt
     2101 taccagatct ccttctgctg cttcttttac ttctagaggg catatcttgt ttagactgtt
     2161 tgcgaattct tcggtaaaac ctgcgggcat atgctgaaca accaaaatgg gttgcggcag
     2221 ggccgggtcc agatgtgcaa acacctcgcg aagcgcgtta ggtccaccgg tagagatacc
     2281 aatggcgata atctctatag gagcgctttc tcgcaatggt ttgggtcgtt cggtaactat
     2341 tcgctgagcg ctcgcagtct cctgtcttct ggggctaagg gatagagggg ctctggaaag
     2401 aaggtcgata gcttgtgcgt gttctttttc gttgtgtcga tcagtgtcca gttccgataa
     2461 accggaacga gcttcccatt ttctgagttc tgcttccggt aatttccggg ccgtgccagc
     2521 gatcttttta agtttatact gattgccata ggcaacaagc aattcaacca gttgattcgc
     2581 tacggtatgg agatttgccg attctgaacc agagggcttg gttacaaagt cgcttgcccc
     2641 aagctcaagg cactccatag ttacacgggc cccttctttt gcgatgctgg acaggataac
     2701 aaccgggata tctataccca gacgctttcg ttctttaaga aactcgattc cgttcatctc
     2761 gggcatttct atgtcaagca cgataatatc ggcttgcgcc cgcagtagtt tttgtagggc
     2821 aaacctgccg ttcattgcct tgtcggctac ggtaagtcct tcgctgtttt caataatttt
     2881 cccaataagg cttctcatca gggcagaatc gtctacgatc aatacagata tggcgtccat
     2941 ggttctcccg ttagctgttt ttttgataga ggcaagccca gtcagtctta agaaattcaa
     3001 actgtgtttt catgccaaag agtgattccg aatgaccgat gaacaaaaag gatttgctcg
     3061 acatcgcgtc ccaaaaccgg tctataaccg ccttttgcgc ggcctcgtca aaatagataa
     3121 ggacattgcg gcaaaagaga atatcaatgt tacgttgccc ggaatcgttt atcaagttgt
     3181 gatagtcaaa acgaatcatc ttcatgattt cgtcttttgc ctgatatcca ctattcttgt
     3241 gctcaaagaa acggtgtaga tagggttcgg gaatgccaac aactcttgct tccgggtaaa
     3301 atcccgactt gcccaccata agcgacttaa gggatatgtc cgacgcggta atctccgcgg
     3361 taaacggcga aggaagttta tcctttagga gcatcgcaag ggtatacggt tcctctccgg
     3421 tcgaacagcc tgcgctccat acccggattt ttgtatcgtt tttagccttt ttgagtttta
     3481 tcacctcggg aataataaag tgttccattg cgtcaaagtg aggttgatta cggaagaacc
     3541 tggtcagatt ggtggtaact gaatcaagca tgagcttgag ttcttctttg tcctttttga
     3601 ggatgctata gtaatcgccc acctcgggta tttgtttttc tctcagtcgt tcccgcaagc
     3661 ggctttccag gattgagcgg tttgttgttg aaaaggtgat tccgctttcg tcgtatatca
     3721 gttttcgata tagttcgaag tcggcgtaac tgagaaaagc ggccataaga actcctgtat
     3781 tggctccagg gaagcctttt tcaagtgtaa gacctcttaa gagggctgtc aattgctact
     3841 atcccctttt gccaggcctc ccaggtgtgc tatagtcaac atatgtcccg cggaataaca
     3901 gcttgtgtcc tttttttctt gttcagcctc ctgctttcct gttccttcaa ttacaccgaa
     3961 ggagatgggg cgggtaaggt gttgcccgaa atggttatag aagctgcccg cgctaccaga
     4021 tatgaggatg cccgaataaa agtggttttt aatgctgatg tcctggagat atacgatagc
     4081 gatcgtgtgt gggcgggaga gaaggtgtct tttgtggaat atgccgccga tgcctccggg
     4141 gctgtggaaa tggaaggggc cgcatcttta ttggtaatag acgataaaga aggcgtgtat
     4201 tcacttggaa acggcgcctt ttttcatctg gttgatgatg atattaaact gactgccgag
     4261 gatcttaagt ggacaaagaa aacccatcag ctttctggaa gcgcggaagg actggtcaag
     4321 atggaagaat ctgacggctc gattattcag ggaacgggtt tttttgccga ctcccttaga
     4381 cggtattatc tttttgactc cacggtatcc ggatttttga tgcgggatgg tagctccgaa
     4441 tgatagcgcg cagtgttttg atgtgtgtgt gtgtaatcgc attgttaacg agtcctcttg
     4501 cggcagaatc aaaaaaaata gcctttaacg ccgaaaagat gagtggcact tccggtaaaa
     4561 aagacagtat taccagtctg gaaggtaacg cgagcgtcac tgtggggact cttacgatta
     4621 aggccgaacg cattgagctg tcggggaagg attttcgcta tgtaaaggcc gacggctcgg
     4681 taaagggctc gg
//
LOCUS       DAOQXQ010000036         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_18981_length_4619_cov_3.010955, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000036 DAOQXQ010000000
VERSION     DAOQXQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18981_length_4619_cov_3.010955"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKH81_01095"
     CDS             <1..291
                     /locus_tag="PKH81_01095"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ96667.1"
                     /translation="LSLLPSVKFSAGIHRGTLILGTIGERERMESTVISDVVNTASRM
                     MEWALASEKCIVISEDIASRLDGANLSWIQPLGPVTLRGRESSTILFEVACP"
     gene            288..2372
                     /locus_tag="PKH81_01100"
     CDS             288..2372
                     /locus_tag="PKH81_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ96668.1"
                     /translation="MTTSIERTHTSRAIFVALIPFACLLALMISLHSANPLSDPDRVS
                     SLTITGGRADLAGSSPDKAFRIRGSWEFYWCHLSDGLDNAIPSEFAPLPGGWDSLSGS
                     PRYGYATYILRLESLIPGHRYAISLGQTISAARLLINGSDIGSIGRPGTSREEEIPGW
                     NYLVSGFTSDAQGHAVLAIQISNHVDRAGGTNALILVGDAQVLEQIRDMQKITEACVF
                     AILATLGCFFLMLYIARAQERSFLWFSLLCISVAVRTLCYDGFVLLDIFPNLPWELFF
                     RLGYLTFTVIICAFFGFLSCLFADVFHSGARRTVFFLFSLYSALIVVAPLSLVVTVLP
                     AFQLLALGTALYGGFIIVRSCRQRREGAYWLLAGFSIALCAFVYDLLVSLWVLSGLSV
                     GHVGMCVFLFVLAVMVIDRYARSYVMVSSLSAELQVINRSLRRFVPDEFLSFLKKGSV
                     TEVRPGDCVEVEMAILSADIRSFTTIAERMGPSEVFVFLNEYLELVGPIVRSNAGFIS
                     KYEGDGFYALFPEGAESAVRASVQIQSAITARNRHKRAVRPLVVGIGLDAGKLTLGTI
                     GDDSRLDGALISTCVRCAGKFEAATKLFSSKILINDAVFMALSDPLAWFLRPVDRLSL
                     GGRTTFLFEIYNNDPEELRDLKWRTQGDLERAMYAWFAGQYDDSRLYLSRIQAVFPDD
                     PVTRYYFRRLLG"
     gene            2507..>4619
                     /locus_tag="PKH81_01105"
     CDS             2507..>4619
                     /locus_tag="PKH81_01105"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ96669.1"
                     /translation="MKKVSTVCILMWFILAAVHAFGSKDTIDTPASTLDSWHETVDIS
                     GRKPGKHNIIVTVEDLAGNQAFGGPFNLYVDPESDLPVARITNPLNNMRVPGNLNIVG
                     TCIDDDAVDYVEIILDNSETPVRAEGKDFWSYYLDTNGMTEGAHTIAVYGVDINGVKG
                     KPFSVTWHLDRNRPETSVQNLTMGALVSKKFTLSGIVTDGNGIKTLAYSLDQGKTFTD
                     LKLDYDKKEFIWKFSVDINTIGMPDGPTVCWFKATDGQGSQGIYTFLYFIDNTPPAVG
                     FISPDPAEAVNGIFSVSGFATDVIGLQSLSWVHGKERGDFEVIKGNPYWVKEFDITGS
                     KDKNFDIEIHAMDIAGNLTVSKQRILVNNDLDVPSLTFTSPAQGELVEGDVLVSGLAI
                     DDDGIAEVWYSLNKSAAEKMDTQGAFGLRLAELPAGSYTVEAWPVDIHGVRGPSVFTT
                     FTAKGSVPRVEIEPVGTPNAELHPEAGGALTAKIFSDAGLKALTWTMTGFEEKTVQIK
                     EGVRDFPLTIAITPDFPWGLLTLEVTATDMYDRKTTSRLPFYITNVGIPRDAPPESSP
                     DTLTVTKEVTIPASGKNPASSGNAVLSIERVLPEDRAFEQGMIVTLAGPGRPKAEQKS
                     AQVRLALQSPLPVSAVNWNLSSGESGKASVQKNDDGSLFALVPLNPLMTPDWTVLTAV
                     ATLKDGTTLEVQGVFCVVRPQS"
BASE COUNT         1022 a         1092 c         1188 g         1317 t
ORIGIN      
        1 ttgtccttat tgccctcggt aaaattctcg gccgggattc accggggcac attaatcctc
       61 ggcactattg gagagcggga gcgaatggaa agcaccgtta tttctgatgt agtaaatacc
      121 gctagtagaa tgatggagtg ggcccttgcc tcagagaaat gcatagtgat aagcgaggat
      181 attgccagtc gccttgatgg agcgaatctg tcatggatac aaccgcttgg tccggttacc
      241 cttcggggta gggaatcttc aaccattctt tttgaggtgg cctgtccatg acaacatcga
      301 tagagcgcac acatacatca agggctattt ttgtggctct tattcccttt gcctgtttac
      361 tcgctcttat gatttccttg cacagtgcaa atcccctctc agatccagat cgggtctctt
      421 ctttgactat taccggcggg cgagccgatc ttgccggaag tagccctgac aaggctttcc
      481 gtattcgcgg gtcctgggaa ttttactggt gccacctgtc cgacggcctt gataatgcga
      541 taccttcgga gtttgcgcct ttgccgggag gatgggactc tctctctggc tctccccgtt
      601 atggttacgc aacctatatt ctccgcctgg aatcgctgat tccagggcat cgatatgcca
      661 taagtcttgg tcaaaccata tcggcagcgc gcctactgat taatggttca gacatcggtt
      721 ctataggccg gccgggtact tcacgggaag aagagattcc cggctggaac tatcttgttt
      781 ccggatttac atcagatgct cagggtcatg cggttcttgc cattcagatt tcaaatcatg
      841 tagatagggc tggaggaacc aatgcgctca ttcttgtagg cgatgcccaa gttctggagc
      901 agatacggga tatgcaaaaa atcacagagg cctgtgtatt cgccatcctt gcaaccctgg
      961 gttgtttctt ccttatgtta tacatcgcgc gcgcacaaga gcggtccttt ctctggtttt
     1021 ctcttttgtg tatctcggtt gcggtgcgca ctctttgtta cgacgggttt gtccttttgg
     1081 atatctttcc gaatcttccc tgggagcttt tctttcggct tgggtatctg acctttactg
     1141 tcataatttg tgcatttttt ggattcctct cctgtttgtt tgcggatgta tttcattctg
     1201 gagcccgtcg gacggttttt ttcctgtttt cactgtatag cgcgcttatt gtcgtcgccc
     1261 cgctctcgct tgtcgtgact gttttacctg cctttcagct acttgctctt ggtacagcct
     1321 tgtatggtgg ttttattatt gtgcgctcct gtcgccaaag gagggaaggc gcatattggc
     1381 ttttggcggg cttttcaatt gccctgtgtg cctttgtata cgaccttcta gtgtcgcttt
     1441 gggttctttc gggtttgagt gtggggcatg tgggcatgtg tgtgtttttg tttgtcctgg
     1501 cggtaatggt gatcgatcgc tatgcgcgct cgtatgtcat ggtgagctct ctttccgcag
     1561 agcttcaggt tattaaccgt tctctccggc gttttgttcc cgacgagttt ctttcttttt
     1621 taaagaaggg ctctgtaacg gaggttcgcc ctggtgactg tgtagaggta gagatggcga
     1681 ttctttcggc cgatattcgc tcctttacca caatcgccga acggatgggt ccctccgaag
     1741 tttttgtatt tctgaatgaa taccttgaat tggttgggcc tatcgtccgt tcaaacgcgg
     1801 gttttatttc caagtatgag ggcgatggct tttatgcctt attcccggaa ggggcggaaa
     1861 gtgcggtgcg ggcttctgta cagattcaaa gcgctattac tgcccgtaac cggcacaagc
     1921 gggcggtacg gccattggtg gtaggcatcg gactcgacgc aggcaagctg acgctcggta
     1981 caattggaga tgatagtcgt ctggacggag cgctcatttc tacctgcgtg cggtgcgcgg
     2041 ggaagtttga ggcagcaaca aagctcttct catccaaaat actgataaat gatgctgttt
     2101 ttatggcatt aagcgatccg ctggcatggt ttttgcggcc cgtagaccga ctgtcgcttg
     2161 gcgggcgtac aaccttttta ttcgagatat ataataatga tccggaagag cttcgagacc
     2221 ttaaatggag aacacagggc gatttggaac gggctatgta tgcctggttt gcaggtcaat
     2281 acgacgactc gcgtctgtat ctatcccgaa tccaggcagt ctttccggat gatccagtta
     2341 cccgttatta cttccggcgc cttttaggct agttaaccgg tttagcaggc ctgtctacta
     2401 cgtcgtttcc caaaaatcaa gacctttaga ctttttttct tgcaaaattt taataatcgt
     2461 gccgataatg gagataatgg agaaggtctc gcgaggagtg tttttaatga agaaagtttc
     2521 aacagtttgt attctcatgt ggttcatcct tgccgccgtt catgcgttcg gaagcaagga
     2581 tacaattgat actcctgcaa gcacgttgga cagctggcat gaaactgtcg atataagcgg
     2641 gcgtaagcca ggtaaacata atattatcgt aacggtcgag gaccttgccg gtaatcaggc
     2701 ctttggcggc ccgtttaact tatatgtaga ccctgaatct gatttacctg ttgcccggat
     2761 aaccaatccg cttaacaata tgcgcgttcc gggaaacctt aatattgtgg gaacctgtat
     2821 tgatgatgac gcagtagact acgttgaaat aatcctggat aacagcgaga ctcccgttcg
     2881 tgccgaaggt aaggactttt ggtcatatta tcttgatacc aatggcatga cggaaggtgc
     2941 acacaccatt gcggtttatg gtgtggatat taatggcgtt aagggcaagc ccttttcggt
     3001 aacctggcac cttgaccgca atcgccccga aacctctgtg cagaatttga caatgggcgc
     3061 ccttgtgtcc aagaaattta ctctttccgg tattgtaacc gacggtaacg gaatcaagac
     3121 cctcgcatat tctctggatc agggaaaaac ctttacggat cttaagcttg attatgataa
     3181 aaaagaattt atctggaaat tctctgtaga cattaatacc atcggtatgc ccgacggccc
     3241 aacggtatgt tggtttaagg cgacagacgg acagggttct cagggtatat ataccttcct
     3301 ctattttatt gataacacac ctccagccgt tggctttatt tctcccgatc ccgcagaggc
     3361 tgttaatggc atcttctctg tttccggttt cgcaaccgat gttatcggct tacaaagcct
     3421 gtcgtgggtt cacggaaagg agcgcggcga ttttgaagta atcaagggaa atccgtattg
     3481 ggttaaggaa ttcgatatta ccggcagcaa ggacaagaac ttcgacatag agattcatgc
     3541 catggatatt gccggaaacc ttaccgtgtc caaacagcgc attttggtaa ataacgactt
     3601 ggacgtacca tcccttacct ttacctctcc tgcgcaagga gaattggtgg aaggggatgt
     3661 gttggtaagc ggcctggcga tcgacgatga cggaattgcc gaggtatggt attcgcttaa
     3721 taagagtgcc gcagaaaaaa tggacacgca gggtgccttt gggctcaggt tggccgagct
     3781 tccggcagga agttataccg ttgaagcctg gcctgtagac attcatggtg ttcgtgggcc
     3841 aagcgtcttt actacattta ccgcaaaggg ttccgttcct cgtgtagaga ttgaaccagt
     3901 tggtacccca aacgcagagc tacatccgga agcgggaggc gcccttactg ccaagatatt
     3961 ctccgatgcg ggcttaaagg ctcttacctg gacaatgacc ggttttgaag aaaaaacggt
     4021 gcagattaaa gagggtgttc gcgatttccc gctgaccata gcgataaccc cggacttccc
     4081 ctggggcttg ttaacgctcg aggttactgc aacagacatg tacgaccgaa aaacgactag
     4141 tcgcttgcct ttctatataa ccaacgtggg tattccgcgg gatgctcctc cggaatcttc
     4201 tccagatacc ttaacagtaa ccaaagaggt taccattccc gcctcaggaa aaaatcctgc
     4261 aagcagcggt aatgcagtgt tgtctattga gcgtgttctt cccgaggatc gtgcctttga
     4321 acagggcatg attgtgactc tagccggccc cggcagaccc aaggcggagc aaaaaagtgc
     4381 gcaggtccgt cttgcccttc aaagtccgct tccggtaagt gccgttaact ggaacctgtc
     4441 ctccggagaa tcggggaagg caagtgtgca aaaaaatgat gacggctctc tctttgccct
     4501 tgtaccgctt aatcccctta tgacacccga ctggacggtg cttaccgcgg tcgcgacact
     4561 taaagacggc actacccttg aggtccaagg cgtattctgt gtggtacgcc cacaaagcg
//
LOCUS       DAOQXQ010000037         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19114_length_4594_cov_2.999119, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000037 DAOQXQ010000000
VERSION     DAOQXQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19114_length_4594_cov_2.999119"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PKH81_01110"
     CDS             <1..152
                     /locus_tag="PKH81_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNQ96670.1"
                     /translation="GRKADFICQEINREINTIGSKNQLLAVGEAVIVCKDALENIREQ
                     LRNIE"
     gene            170..733
                     /locus_tag="PKH81_01115"
     CDS             170..733
                     /locus_tag="PKH81_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNQ96671.1"
                     /translation="MGGFHVVQGKEVRVAISGRSGCGNTTVSRLLAEILDIAFINYTF
                     RSLSQETGMPLSEIIEKAKTDFTFDRQVDTRQVALAQEASCVLGSRLAIWMLEKADLT
                     VYLIASEEVRAKRISNREGASIQETISFTAMRDAEDTRRYRELYGIDNTNYSFADMVI
                     DTEQKNPEEIVSDILQELEKRGLVARD"
     gene            726..1547
                     /locus_tag="PKH81_01120"
     CDS             726..1547
                     /locus_tag="PKH81_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNQ96672.1"
                     /translation="MIKKSLNIDQFRQTILEHYHQEKREFPWRETNDPYAILTSEIML
                     QQTQTDRVVPKYLAWLDSFPNIHALAQAETTEVLERWVGLGYNRRAIFLHRAAKIMDS
                     EYNGFVPQELTDLLSLPGIGPYTARAIRTFAWGYPEVFIETNIRSVFLFFFFPDQTAI
                     DDSNIMPLIESSLYLEDPRTWYYALMDYGSVLKRKTINPNRNSKHYSKQSRFTGSNRE
                     ARGAIVRTLSTGARYTITDLATQCGLDRERITKALLGLTKEGIVCDMDGVYGIYE"
     gene            1610..1681
                     /locus_tag="PKH81_01125"
     tRNA            1610..1681
                     /locus_tag="PKH81_01125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1643..1645,aa:Gly,seq:tcc)
     gene            1794..3305
                     /locus_tag="PKH81_01130"
     CDS             1794..3305
                     /locus_tag="PKH81_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ96673.1"
                     /translation="MSDSSLYDSFIGTTGSYTCIISPSGVIESISDPFAHLLALRHKE
                     DLVGINAIDAVQGSVELKTLFTDICNVSPKTEIPSTRLVHVHRNAEKPLWIKFDIRSN
                     VHQGKDAGIVLTGLDVTDLVISQKDAEKKALQRSSFLVKMGHELRTPLNTVLGYAQLL
                     SGLTDLSPTAATYTQVILTHENSLLRILNDILEYSKYESGQITPVYTEVNLRKLIQDV
                     TKSYIEQCNAKTVALTVELSPTIPETIQSDSIKLTQILSNLLANALTNTRKGGITVTV
                     SCDRVITIDVEDTGTGIPLHEQDTIFEVYATDEEGKELPRGSGIGLAVARLFARTLGG
                     DVTLVRSDSLTGSLFRCTLEARESSSKKRELQQITDYTTIHGLSRPCKVLLVDDVDIN
                     LAMLEIFLAPAGFNVSIASNGEEAVNIFKQFKPDIVFMDLIMPGKNGFDATRDIKNID
                     GSVPVIALTASIVDSIKEQALQAGVNDFMYKPFIPERFFEIIAEHTGISYVTN"
     gene            complement(3308..>4594)
                     /locus_tag="PKH81_01135"
     CDS             complement(3308..>4594)
                     /locus_tag="PKH81_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96674.1"
                     /translation="KIRLLFSNERVFDSSEVIALYGEADSFGIVPVFLTVRTEDTINI
                     IALPYPKYNSNDGLSLKIKIKNYNFFGSMQELDTEFVYESDIDGDKSIEGGFSFIIPF
                     VAYTYECIWDIASSLTWHFESDPEFDLNTGLDVAFPLSFADLHLGFVQSIHINDRDDD
                     NEQYVDDLYFSERLYVKLPITLYETDSFGDVVWTPYISFLANWDSDGISSEDLKGPDV
                     RFGQTISVGQIDWDRNFRNGVSAEAGVSWTHNFNSPQRKEVTIDSTIKAYKSFYDRLA
                     LYTRLRGFYNFDDFISEKQGVMLRGILDKRIQTDTAVYLNIDIPVRVMRVDFLEVTGV
                     SWTRYISFEMHVSPFFDMAITHDLDTGSQYSFADGWYAGGLEVIVFPLKMRSIYARAS
                     VGFDLVEAWESGNLSGESKRDGESIREISIGLGFQY"
BASE COUNT         1419 a          971 c          909 g         1295 t
ORIGIN      
        1 ccggacgcaa agctgatttt atttgccaag aaattaatcg tgagattaat accattggtt
       61 ctaagaatca gttacttgcg gtaggagagg cggtaattgt ttgtaaggat gccctggaaa
      121 atattcgaga acaattgaga aacatagaat aagggagcaa gtgactataa tgggcggttt
      181 tcatgtagtt caggggaaag aagttcgcgt tgccatatca ggaagatccg ggtgtggaaa
      241 tacaacggta tcaagattat tagccgaaat ccttgatatt gcatttatca actatacatt
      301 tcgatcgcta tcccaggaaa ccggtatgcc cctgtcggaa ataattgaaa aggcaaaaac
      361 agattttacc tttgacagac aagttgacac tcggcaggtt gctcttgcac aagaagcttc
      421 ttgcgtactt ggttcccgtt tagcaatatg gatgctagaa aaagccgatt taaccgtcta
      481 tttaatagcc tcagaggaag ttcgagctaa acgcatatct aatcgtgaag gagcatcaat
      541 ccaagaaaca atcagcttta ctgccatgag ggatgctgag gatacacgac gatatcgcga
      601 attgtacgga atagataaca ccaattattc atttgcggat atggtaattg atactgaaca
      661 aaaaaatcct gaggaaattg tatcagatat tttacaagaa ctggagaaac gaggtcttgt
      721 agcgcgtgat taagaaatca ctgaatatcg atcaatttag acaaactata ctcgagcact
      781 atcatcaaga aaaaagggaa ttcccttggc gagagacgaa tgatccctat gccattctta
      841 cctccgaaat tatgcttcaa caaacgcaaa cagacagggt cgttccaaaa taccttgcat
      901 ggcttgattc atttccaaac attcatgcac ttgcacaagc tgagacaaca gaggtgttgg
      961 agagatgggt agggcttggc tataaccgca gggctatctt tcttcatcgc gctgcaaaga
     1021 tcatggatag tgaatataat ggctttgtac cacaggagct taccgatctt ttatcccttc
     1081 ccggaattgg tccatataca gctcgagcga taagaacttt tgcctgggga tacccggagg
     1141 tttttattga gacaaatatt cgatcagtgt ttttgttttt tttctttcct gaccaaacag
     1201 ccatagacga ttctaatatt atgcccctaa tagaatcttc actctatctt gaagatccaa
     1261 gaacctggta ttacgcactt atggattatg gatctgtatt aaaacgaaaa acgatcaatc
     1321 ccaacagaaa tagcaagcat tatagtaaac agtctcgttt taccgggtca aaccgtgaag
     1381 cacggggcgc aatagtcagg actctttcaa ctggtgcccg ttatacaatt accgatcttg
     1441 ctactcaatg cggtttggat agggagcgta ttacaaaggc tcttttgggg cttaccaaag
     1501 aaggaatcgt ttgtgacatg gacggggttt atggaattta tgaatagctc agacttgaca
     1561 cacagcgctt ttttttgata taaattcata cataataaga cccgacagag cggatgtcgt
     1621 ataacggcta ttaccccagc cttccaagct ggagacgagg gttcgactcc cttcatccgc
     1681 tctgtcgggt ttttttttta ccgccttcca ctactatcca cacttcgttg acagagcata
     1741 tagcgggtgc tatcatatta atcagttatt acattaacaa caggtggtac gagatgtccg
     1801 actcctccct ttacgactct tttattggta caactggttc ttatacctgt attattagcc
     1861 cttcaggagt aatagagtct atcagtgatc cttttgcgca cctcttggca ttacggcaca
     1921 aagaagacct tgttggaata aacgctatcg atgccgttca gggatcagtt gaattaaaaa
     1981 cattatttac tgatatatgt aatgtatctc caaaaactga aatcccatcc acccgcttgg
     2041 tacatgttca tcgtaatgca gaaaaacctt tatggattaa gtttgatatt cgctctaatg
     2101 tacaccaagg aaaagatgct ggaattgtat taactggact tgatgttaca gatcttgtta
     2161 tatctcaaaa ggatgccgaa aaaaaagccc tccaaagaag tagttttttg gtgaaaatgg
     2221 gacatgaact taggacccca cttaacaccg tgctgggcta tgcgcaacta ttatcgggtc
     2281 ttaccgattt gtccccaacg gcagctacat atacacaggt aatcctgact catgaaaaca
     2341 gcctgcttcg tattctcaat gatattcttg aatactcaaa atatgaatca ggacagataa
     2401 ctccggttta caccgaggtt aacttacgaa aattaattca agacgtgacc aagagctata
     2461 tcgagcaatg taacgcaaaa acagttgccc ttacggtcga attgtcaccg actatccccg
     2521 aaacgattca aagtgattca atcaaactta cccaaatttt atctaattta ctcgctaacg
     2581 cccttaccaa caccagaaag ggcggtatta ccgttaccgt ctcttgtgat cgggtaataa
     2641 caattgatgt agaggatacc ggaactggta tcccgctcca tgaacaagac actatttttg
     2701 aggtatatgc tacagatgaa gagggtaaag aattgcctcg aggaagtggc atcggtcttg
     2761 cagtagctcg attgtttgcc cgaacccttg gcggagatgt tacacttgta cgctccgaca
     2821 gccttaccgg atccctcttt cgctgtacct tagaggcccg tgaatcttcc tcaaagaaac
     2881 gtgaactcca gcaaattact gattatacta ccattcatgg tctatcccgt ccttgcaagg
     2941 tcctgttagt ggatgatgtc gatataaacc ttgcaatgct cgaaattttc cttgcacccg
     3001 ccggatttaa cgtaagcata gcctcaaatg gcgaagaagc ggtcaacatc tttaaacaat
     3061 tcaaaccaga tattgttttt atggatttaa ttatgccagg taaaaatgga ttcgatgcaa
     3121 caagagatat aaagaatatc gatggatcgg tgccagtaat cgcgctaacc gcaagtattg
     3181 tggattctat caaggaacag gcacttcagg cgggggtcaa tgattttatg tacaagccct
     3241 ttataccgga gcgttttttc gagataattg ccgagcacac cggtattagc tatgtaacaa
     3301 actgatccta gtattgaaaa ccaagaccaa tggatatttc tctgatagat tctccatccc
     3361 tttttgactc cccggagaga ttcccacttt cccaggcttc taccagatca aaccccacac
     3421 ttgctctagc ataaatactt ctcattttta atgggaaaac aatcacctca aggcctcctg
     3481 cataccaacc gtctgcaaaa ctatactgtg atccagtatc aaggtcatga gtgatagcca
     3541 tatcaaaaaa gggagataca tgcatttcaa aactgatata ccgtgtccag gacacaccgg
     3601 ttacttcaag aaaatcaacc cgcataacgc gaacagggat gtcaatgttt aggtatactg
     3661 cggtatctgt ttgaatacgt ttatcaagaa ttcctcgaag cataaccccc tgtttttctg
     3721 aaataaagtc atcaaagtta taaaatcctc gaagacgagt atacagagcg aggcgatcat
     3781 aaaagctttt gtatgctttt attgttgaat caattgtcac ttccttacgt tggggtgaat
     3841 taaagttatg tgtccaagaa actcccgctt cggcacttac accatttcga aagtttcgat
     3901 cccaatctat ctgtccaact gatatagttt gtccgaaacg tacatcggga ccttttaaat
     3961 cttctgaact aataccatca ctatcccaat tcgctagaaa ggatatatat ggcgtccata
     4021 ctacatcacc aaaagaatca gtttcataaa gggtaatcgg taatttaacg tataatctct
     4081 ctgaaaaata gagatcgtct acatactgtt cgttatcatc atcacgatca tttatgtgga
     4141 tgctttgtac aaaaccaagg tgcaagtccg caaatgaaag aggaaaggca acatccaaac
     4201 ctgtgttaag atcaaattcc ggatcacttt caaaatgcca ggttaatgat gaagcgatat
     4261 cccaaataca ttcgtacgtg tatgcaacaa agggtattat aaaagagaat ccaccttcta
     4321 tacttttatc tccatcaata tcagattcat acacaaactc tgtatccaac tcctgcatac
     4381 tgccaaagaa gttgtaattt ttaattttta tttttaagga aaggccatcg ttggaattat
     4441 attttggata aggaagggct atgatattga tagtatcctc tgtgcgaacg gtaaggaaga
     4501 ctggcactat tccaaaactg tctgcttccc cgtacagggc aataacctcg gaggaatcaa
     4561 agacccgttc attagagaac aagagtctta tttt
//
LOCUS       DAOQXQ010000038         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19243_length_4574_cov_3.565169, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000038 DAOQXQ010000000
VERSION     DAOQXQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19243_length_4574_cov_3.565169"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..161
                     /locus_tag="PKH81_01140"
     tRNA            88..161
                     /locus_tag="PKH81_01140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Arg,seq:tcg)
     gene            223..295
                     /locus_tag="PKH81_01145"
     tRNA            223..295
                     /locus_tag="PKH81_01145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:256..258,aa:Lys,seq:ctt)
     gene            377..456
                     /locus_tag="PKH81_01150"
     tRNA            377..456
                     /locus_tag="PKH81_01150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:411..413,aa:Leu,seq:tag)
     gene            499..570
                     /locus_tag="PKH81_01155"
     tRNA            499..570
                     /locus_tag="PKH81_01155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:531..533,aa:Gly,seq:gcc)
     gene            684..2051
                     /gene="tig"
                     /locus_tag="PKH81_01160"
     CDS             684..2051
                     /gene="tig"
                     /locus_tag="PKH81_01160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNQ96675.1"
                     /translation="MNLKKQLTKLDKSRVKLDVTIAKDDLAAAYQESLQKHAKNITLP
                     GFRKGKVPVSILEKKYGEALKAEAASDIMEKALGEVFESASETERPLPYSQPTMEEAP
                     EFSLDKDLSFSVTFDVFPSVTLSTIEGFKIELPQVTVGDNELKEELEAIRERNALVVE
                     KNDGDKAAKDDIVTVNYCELDDAGATVPASEREDFVFTIGTGYNIYKFDDEIIGMKKG
                     DTKEFSKKFAETEEDKDLAGKTKKLKVTLTALKSRKLPELDDELAQDVNEKYKTLEDL
                     KSDIRKNMEVALENKMKEIKSNTLLEKIVEANPFELPESMVQAELESRWRMLAQRFQT
                     DAAQLEKLIISSGQSKQSMLESWREEAEKLLKSRVVVEILLKDRDIAVTPEDVEAEYA
                     SIAERAGISVDEVKKHYADMRRKEYLIDDIKEQRLYGQLLEKCTMAKGKKTAFAELFK
                     DGNQQ"
     gene            2048..2665
                     /locus_tag="PKH81_01165"
     CDS             2048..2665
                     /locus_tag="PKH81_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNQ96676.1"
                     /translation="MNEQMNNLVPYVIEQTGNGERSYDIFSRLLKDRIVFIDGEITDA
                     TADLVVAQLLFLDSQNPEKDINLYINSPGGSVTAGLAIYDTMQQIRSEIQTFCLGQAA
                     SMAALLLAGGSPGKRSALPSSRVLIHQPWGGAQGQAVDIGIQAREIIRLKKLTIEYFS
                     YHTGKTVEEIAQGMERDFFMSPQEAVEYGIVDKVMTRRKHGKTEE"
     gene            2646..3890
                     /gene="clpX"
                     /locus_tag="PKH81_01170"
     CDS             2646..3890
                     /gene="clpX"
                     /locus_tag="PKH81_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNQ96677.1"
                     /translation="MAKLKNDKEQICSFCGKTADTSRRIIAGPGVFICDHCVDVCKGI
                     LDEENQQLSTEFTGDIPTPMEMKAHLDQYIIGQEQAKKTLSVAIYNHYKRIANPSKKP
                     EDVVIEKSNVLMIGPTGSGKTLLARTIAQKMKVPFAIADATTLTEAGYVGEDVENILL
                     KLIQNSGGNIAAAERGIIYIDEIDKIARKGENVSITRDVSGEGVQQALLKIIEGTTAS
                     VPPQGGRKHPNQEMIKIDTTNILFICGGAFVGLDKMIESRIAQHPMGFGADIKIQKER
                     NLQELFNHIAPDDLIKFGMIPEFIGRLPITLALNELVRDDLRRILTEPKNSIVRQFQA
                     SLDIDKVALVFEDDAIDAIADLAIKQNTGARGLRAIVEKMMMELMYEIPSIEGNKRVV
                     ITKEVVEKNRKPEITMADQKTA"
     gene            complement(3950..>4574)
                     /locus_tag="PKH81_01175"
     CDS             complement(3950..>4574)
                     /locus_tag="PKH81_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ96678.1"
                     /translation="DTSLFYPHPVENNEAVLIHPFSIRRPYIIYASRVAYPGKCHVEL
                     IRAFELFKKKTGSPHRLVLAGSDGINAEMVHREVLKSSVAPDILLTGYFPHQNLSELY
                     SAADACIFPSATEGVGLPVLEAMASGIPVACARSGALPEMAADAALYFDQNKPEEIAD
                     CIERLVRLPSGENDSLRREMIAKALERSTQFSWASCAEQTIRTLDSL"
BASE COUNT         1325 a         1059 c         1139 g         1051 t
ORIGIN      
        1 gctgtcgtgg gttcgaaacc catcacccac ccttggaaaa cgttcagaac catctgagct
       61 gttttccgcc cgttttacgg gatgtatgcg cccgtagctc agctggatag agcaccggac
      121 ttcgaatccg taggtcgccc gttcgaaccg ggctgggcgc agaggcgcaa aacgcgcctg
      181 attgacaaaa aatcgtgttt actgtaaaac agtaatccgc acgggccatt agctcagctg
      241 gtagagcaac agactcttaa tctgtgggtc gaaggttcga tcccttcatg gctcaaagaa
      301 ctgtacagaa ggtgcttgac acagtctttc cggttcggta gtatgttgaa actcttaagt
      361 aggattccga acaagagcga gagtggtgga attggcagac acgccagact taggatctgg
      421 tgcctaggcg tgagggttca agtccctcct ctcgcatttc cgccagcgtg accaagatag
      481 gtgctttagt acgattttgc gggaatagct cagtggtaga gcgcgacctt gccaaggtcg
      541 atgtcgcggg tccgactccc gtttcccgct cttactcgga aggcgatccg gtagaatccg
      601 gatcgccttt tttttatgcc tcctgacaag ggggcaaaca cataccggta cttttacacc
      661 aaaaccatag aaaggacaca caaatgaacc tgaagaagca gttaaccaaa ctcgacaaat
      721 cacgcgtcaa gctggacgtt accattgcaa aagatgatct tgcagcggca tatcaggaat
      781 ctctgcaaaa acatgcaaag aacattaccc ttcccggttt ccgcaagggt aaagttcctg
      841 tatccattct ggaaaaaaaa tatggcgaag ccctaaaggc agaagccgca agcgatatca
      901 tggaaaaagc cttgggtgag gtttttgaat ccgcttccga aacagagcgc ccgctcccct
      961 actcccaacc aacaatggaa gaagcacctg agttcagtct ggataaggac ctttccttta
     1021 gcgttacctt cgacgtattt ccgtcggtga ccctttcaac tatcgaagga ttcaagattg
     1081 agcttcccca ggttaccgtt ggagacaatg aactcaagga agaacttgaa gcaatccggg
     1141 agagaaatgc ccttgtagta gagaaaaacg atggagataa ggctgccaag gacgacatcg
     1201 ttactgttaa ctattgtgaa ctcgatgatg caggggctac tgttcccgca agcgagcgag
     1261 aagattttgt atttaccatc ggcaccggct ataacatcta taaattcgat gatgaaatca
     1321 ttggtatgaa gaaaggcgac accaaggaat tctcaaagaa attcgccgag acggaagaag
     1381 ataaagacct tgccggaaag accaaaaaac tcaaggttac ccttaccgcc cttaagtctc
     1441 gcaagcttcc tgaactggac gatgaacttg cccaggatgt aaacgaaaag tacaagactc
     1501 ttgaagacct gaagagcgac attcgcaaga atatggaagt cgccctggaa aacaagatga
     1561 aggaaataaa gagcaatacg cttttagaaa aaattgttga ggcgaatcct tttgagcttc
     1621 ctgaatccat ggttcaggca gaacttgaat cccgctggcg tatgctcgca cagcgattcc
     1681 aaaccgatgc cgcccagcta gaaaagctta ttatctcatc cggccagtcc aaacagtcca
     1741 tgcttgagtc ctggagggaa gaagcggaaa aacttcttaa aagccgtgtt gtggtggaaa
     1801 tactcctcaa ggacagggac attgccgtta caccggagga tgtggaagct gaatatgctt
     1861 caatcgcgga acgtgcagga atttccgttg atgaggtaaa aaaacactat gcggatatgc
     1921 gtagaaaaga gtatcttatt gatgacatca aggagcagcg cctgtatgga caacttcttg
     1981 agaagtgcac catggccaag ggcaagaaaa ctgcctttgc ggaactcttt aaagacggga
     2041 atcaacaatg aatgaacaga tgaacaatct ggtaccttac gttatagagc aaaccggaaa
     2101 cggtgagcgc tcgtatgata tcttctctcg gcttcttaag gacaggattg tttttatcga
     2161 tggggaaatt actgacgcaa ccgcagattt ggtggttgct cagcttcttt ttttggattc
     2221 gcaaaatccg gaaaaagaca ttaatctcta tatcaacagt cccggcggtt ctgttaccgc
     2281 aggacttgcc atatacgata ccatgcagca aatacggtcc gagatacaaa ccttctgcct
     2341 tgggcaggcg gcgagtatgg cagccctttt gcttgcagga ggctctcccg gcaaacgctc
     2401 ggcgcttccc tcttcccggg ttttgattca tcaaccctgg ggtggagcgc agggccaggc
     2461 cgtcgatatc ggtatccagg ctcgggaaat tattagactc aagaaattaa ccattgagta
     2521 tttttcctac cataccggca agacagtcga agaaattgct caggggatgg aacgggattt
     2581 ttttatgtca ccccaggaag ctgttgaata cggcatcgtg gacaaggtga tgacccggag
     2641 gaaacatggc aaaactgaag aatgacaaag aacagatttg ttctttttgc ggtaagaccg
     2701 cggatacctc acgtcgcatc atagcgggac ccggtgtatt tatctgtgat cactgtgtag
     2761 acgtgtgcaa gggtattctt gacgaggaaa accagcaatt atcgaccgaa tttaccgggg
     2821 atattcccac gccaatggaa atgaaggccc atcttgatca atatattatc ggtcaggagc
     2881 aggccaagaa aacgctttcc gttgccattt ataaccacta caaacgcatt gcaaacccct
     2941 caaagaaacc cgaggatgtg gtaatagaaa aatcaaacgt cttgatgatt gggcccactg
     3001 gctctggcaa gacactgctt gccagaacaa tcgcgcaaaa gatgaaagtc ccctttgcga
     3061 ttgctgacgc aaccacgctc acagaagctg gatacgttgg cgaagacgta gaaaacatcc
     3121 tgctcaagct tattcaaaac tcgggcggga atattgccgc agctgaacgc ggaataatct
     3181 acatcgacga gatcgacaag atagcccgta agggagagaa tgtttccatc acacgcgacg
     3241 tttccggaga gggagtacag caagcccttc ttaagattat cgaaggcaca actgcttcag
     3301 tgcccccaca gggcggacga aagcatccga accaggagat gatcaagata gacaccacca
     3361 acatattgtt tatctgtggt ggcgcctttg taggactgga caagatgatc gaaagccgaa
     3421 ttgcccagca tccaatgggt tttggcgctg acattaaaat ccaaaaagag agaaaccttc
     3481 aggaactgtt taaccacatc gctcctgatg acttgatcaa atttggtatg attcctgagt
     3541 ttatcgggcg acttcctatt acccttgccc ttaacgaact tgttcgagat gatttgcgcc
     3601 gtatcttgac cgagccaaag aactccattg ttcggcaatt tcaagcctcg cttgacatcg
     3661 acaaggttgc ccttgtgttt gaagatgacg caattgatgc aattgcagat ttggcgatta
     3721 agcaaaacac cggagcccgt ggactacgcg ctattgtaga aaagatgatg atggaattga
     3781 tgtacgaaat tccgtccatc gaaggaaaca agcgagtggt gataacaaag gaagtggttg
     3841 aaaaaaaccg caagccagaa atcactatgg cggatcaaaa gaccgcttag actctttctt
     3901 ctgaaataca aaggccggta atcagctaga agctgattcc ggcctttttt tatagggaat
     3961 caagggtgcg tatggtttgt tctgcgcaag atgcccagct aaactgagtg cttctctcca
     4021 aggccttagc gatcatctcg cgtctaagtg aatcgttttc cccggaagga agacgaacca
     4081 agcgttcaat gcaatcggcg atttcttcag gtttattttg atcaaaatag agggcagcat
     4141 ctgcggccat ttcgggtaag gcgccggagc gagcgcaagc aacgggtata ccggatgcca
     4201 tagcttcaag cacagggagc cctacccctt ctgttgcaga agggaagata caagcgtcgg
     4261 cggcagaata caattcactt aaattctgat gcggaaaata tccggtaagc aggatatccg
     4321 gggccaccga agacttaaga acctcgcggt ggaccatctc ggcgttaatg ccgtcagaac
     4381 ctgccaagac gagtcgatgc ggggaaccag tttttttctt gaacagttca aaagctcgaa
     4441 ttagctcaac gtgacacttt cccgggtagg ccacacggga tgcgtagatg atatagggac
     4501 ggcgaatgga aaaaggatgg ataagaactg cttcgttatt ttccaccgga tggggataaa
     4561 acaaagaggt gtca
//
LOCUS       DAOQXQ010000039         4531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19491_length_4531_cov_2.740617, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000039 DAOQXQ010000000
VERSION     DAOQXQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_4531_cov_2.740617"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1110)
                     /locus_tag="PKH81_01180"
     CDS             complement(43..1110)
                     /locus_tag="PKH81_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNQ96679.1"
                     /translation="MGRVVLGADSLDGYLTDKDTSYLSRMDVLYARAMESFKILAAGG
                     FSSTLANEEKKVIDIFNEMGHVMQEICKEAPGIKVYSFETEEQSHAEASRVIAKLRDI
                     RTGHKEFVYYTQRAYEMLFRLAYNRNHSDNKNYLVVKTPVTAPVQNYAVHKIPDIDYK
                     IENSVMCVLLRGALLPSMIMSKEIEEYSSHGYVTPFALFKIKRDDSKKEKNMEYILDL
                     DKSYFNMEDLNGRDLIFADPMNATGGSLVTVVKYLQENGVKPKSISFFNVISALKGAL
                     RVVRALDNCTVYTLWMDPVLNASAYIMPGLGDAGDRINGKDTDESPRNIIQLIADYGS
                     DIAGLYRSQLREIEHIVLGRH"
     gene            complement(1192..1686)
                     /locus_tag="PKH81_01185"
     CDS             complement(1192..1686)
                     /locus_tag="PKH81_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96680.1"
                     /translation="MNKKILALVALLLIFTATGAFAFGIGLQYNGNASRVFTNGVAVT
                     FKVDSIPLIFAANWNFADDSTEFGFTGDYWVLNNKITNVGSASLNWFLGIGFFANFAF
                     PDDEFYFAWGGRIPLGLNMFIAKGVFEPFLQVAPSFGIQVIPNLDTTDLFFPISAGFR
                     IWFK"
     gene            complement(1809..2909)
                     /locus_tag="PKH81_01190"
     CDS             complement(1809..2909)
                     /locus_tag="PKH81_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96681.1"
                     /translation="MKKLVCSLCYVVFLTICSIHSAVPNTAVWELLTEAEILFESGEF
                     GKALTEAERARTFHQDLINGYSRLLSDALIPREVKKAGTEIGPVRDVLLKRNEIEAVG
                     IVDMLIDLKGKDFFKNSIPLMLTWLSDRSVLPEADILCGMIYEAEGEYRMASSYFLSA
                     WKHRAFLDVPSRRFEILYRLADMSHVQGEEGRREEYLLLVLAEDAVYSPSQGGQTSLR
                     AMINTLESNQDTEKFFSLYRHNQQVSLKAARDLTDFYYSSSGRLDRALQTATLASIIS
                     LTLLDNALRQKDFEYTWAGFTDMMKRIGKTPSIMQWAQEEDIWQSFLLLALIVGDRGK
                     HALSESILMDLASSCPDPVIVTKARSRLNTIP"
     gene            complement(2906..4024)
                     /gene="ispG"
                     /locus_tag="PKH81_01195"
     CDS             complement(2906..4024)
                     /gene="ispG"
                     /locus_tag="PKH81_01195"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNQ96682.1"
                     /translation="MSDFLKSRLITIGGKGKTRALSIGADAPVSIQTMWKSPLVGADI
                     HDIARKLSLLEQLGCDIVRFAVPDSASAGLLCQLAEVTSMPLVADIHFDWRLALACMD
                     GNVAKIRINPGNIGSEDRIAEVVKKAQDTHTALRIGVNSGSLPKDLQACVNDAINAGK
                     DINQARAHALVEAASREVEFFDQLSFTTFLVSMKASSVQETVYSNEEFARRFNHPLHL
                     GVTEAGPLIAGVVKSTLAFSSLLTRGIGSTVRVSLSDNLENEVIAAREILSECGLRKG
                     GVRIVSCPRCGRDGFDVHGFVSRWQRELYSLNKNITVAVMGCIVNGPGEGKHADIGIT
                     GAGSEVVIFRHGEIVRRVSTKDGYTETDNAFRQELEKL"
     gene            complement(4135..4518)
                     /locus_tag="PKH81_01200"
     CDS             complement(4135..4518)
                     /locus_tag="PKH81_01200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNQ96683.1"
                     /translation="MSAIGSAIGLAIAGQATVGSWKRCYMNNKPAPFILVAFAGAPLT
                     QTIYGFLLMNAMLLSKKDPWYLLGVGLVCGAAIASSAIAQGTASAAGADALGETGKGF
                     SQYITIVGLCETVALFVMVFGLISV"
BASE COUNT         1378 a         1069 c          935 g         1149 t
ORIGIN      
        1 tggcgataat cagcaaacag agaacaggga gaagacgatt cattagtgtc tgccgagtac
       61 gatatgctct atttctcgta attgagagcg atacagaccc gcgatatccg agccataatc
      121 agcgataagc tgaatgatat ttcgtggaga ttcatcggta tccttaccgt taatcctgtc
      181 cccggcgtcg cccaggcctg gcataatgta ggccgaagca ttcaatacgg gatccatcca
      241 gagtgtatac accgtacaat tatcaagagc acgaactacg cgcaaagcac ctttaagcgc
      301 ggaaatcacg ttaaagaagc tgattgactt tggtttaacc ccattttcct gcaaatattt
      361 aacgacagtt accaagcttc caccagtggc attcatggga tcggcaaaaa taaggtcacg
      421 accattgagg tcttccatgt taaaatatga tttatccaaa tcaaggatgt attccatatt
      481 tttttctttt ttagaatcat cgcgttttat cttgaacaag gcaaagggag taacatatcc
      541 atgtgaagaa tattcctcaa tttccttgga cataatcatt gaaggaagca aggcccctct
      601 tagcaacaca cacataaccg agttttctat tttataatca atatcgggta ttttatgtac
      661 cgcataattt tgcacaggag cggttactgg tgttttaacc accaggtaat tcttgttatc
      721 ggaatgattg cgattatatg ccaatctaaa cagcatttcg tatgctcgtt gagtgtaata
      781 cacaaattct ttatgcccgg tgcgaatgtc cctgagcttt gcgattaccc gagaagcctc
      841 ggcatgactt tgctcttctg tttcaaagga atacacttta attcctggag cttccttgca
      901 gatttcctgc ataacgtgcc ccatctcatt aaagatatca atgacttttt tttcttcatt
      961 tgccagagtt gaactaaaac cgcccgcggc gagtatcttg aaggattcca tcgctcgtgc
     1021 gtaaaggaca tccatcctgg aaagatagct cgtatcttta tctgttaaat acccgtcaag
     1081 agaatcagct cccaatacaa ccctgcccat atagactcct tatggttatt atatggtgat
     1141 actaccataa aaaaaccgtc ccgaaaaggg acggtttaag tttgttacac gttatttaaa
     1201 ccaaattctg aaacccgctg atataggaaa gaatagatct gtggtgtcca agtttggaat
     1261 gacctggata ccaaaggatg gcgcaacttg caagaatggc tcaaagacac ccttagctat
     1321 aaacatgttt aacccaaggg gaatacgacc accccaggca aaataaaact cgtcatcggg
     1381 aaaggcgaaa ttagcaaaaa aaccgattcc caaaaaccag ttcaaactag ctgaacctac
     1441 attggttatc ttgttgttca agacccaata atctccggta aacccgaatt cagtagaatc
     1501 gtccgcaaaa ttccagttcg ccgcaaaaat aaggggaatg gaatccacct taaaggttac
     1561 ggcaactcca ttggtaaaca cccgtgaagc gttaccgtta tactgaagcc cgatcccaaa
     1621 agcaaaggct ccagtagcag taaagatgag caataaggct acgagggcaa ggatcttttt
     1681 attcataaaa tactcctatc gattgcagtt tccggctaac acctagctgg atgtacttcc
     1741 aataacatca agactaccgc catttctgtt acatttcaac atctgctgtt acaatccgca
     1801 ccattttatc agggaatcgt attgagtcga gaccgagctt ttgtaacaat aacgggatcg
     1861 gggcaacttg aggccaagtc cataaggata gactcggaaa gagcgtgttt acctcgatca
     1921 ccgacaataa gagcaagaag taaaaaagat tgccagatat cttcttcttg ggcccactgc
     1981 ataatacttg gggttttgcc tatccgcttc atcatgtcag taaaacctgc ccaggtatat
     2041 tcaaaatctt tttgacgcaa ggcattatca agcaaggtta atgaaataat acttgcaaga
     2101 gtagcggttt gaagtgcacg atcaagtctt cctgatgatg aataataaaa atcagttaaa
     2161 tcccgagctg ccttaagcga tacttgttga ttgtgccgat agagcgaaaa aaacttctct
     2221 gtatcttggt tggattccag ggtattgatc attgcacgca agcttgtctg cccaccttgt
     2281 gagggagaat agaccgcatc ctctgccaaa acaagcaata gatactcttc tcgccggcct
     2341 tcttctccct gaacatgcga catatctgct agccggtaaa gaatttcaaa acgtcttgaa
     2401 ggtacatcca aaaatgcacg atgtttccaa gcggacaaga aatatgaact ggccattcga
     2461 tactcgcctt cggcctcata aatcattcca cagagaatat cagcttctgg tagcacagac
     2521 cggtcagaaa gccaggtgag cattaacgga atagagttct tgaaaaaatc ctttccctta
     2581 agatcaataa gcatatcaac tattccaaca gcctcaattt cgtttcgctt aagcaataca
     2641 tcgcgaactg gcccaatctc agtccctgct ttttttactt cccggggaat aagggcatca
     2701 gacaacagtc gtgaataacc gttaatgaga tcttgatgaa aggttctcgc tcgttctgcc
     2761 tcggtaaggg ccttgccaaa ttcaccactt tcaaaaagaa tttccgcttc ggttaaaagt
     2821 tcccatactg cagtattagg taccgctgaa tggatagagc aaatagtgag gaaaacaaca
     2881 taacagaggg aacacacaag tttcttcaca gtttttccag ctcctgtcta aaggcattat
     2941 ctgtttctgt atagccatct tttgtagata cacgacgaac aatctcgcca tgacgaaaaa
     3001 tgacaacttc ggatccagca ccggtaatgc ctatatcggc gtgtttccct tcgcctggtc
     3061 cgtttacaat acagcccatt acggcaacgg taatgttttt attaagagaa tacagttccc
     3121 gctgccagcg ggaaacaaaa ccatgaacat caaaaccatc gcggccgcag cgaggacagg
     3181 agacaatacg cactccaccc tttcttaagc cacactcgga aagtatttct cgtgcggcaa
     3241 taacctcatt ttccagatta tcagacaaac tgacccttac ggttgaaccg attccccggg
     3301 tcaaaagact actaaaggct aaggtacttt ttaccacacc agcaataagt gggcctgctt
     3361 ctgttacacc cagatggagg ggatgattga atctccgggc aaattcttcg ttggaataca
     3421 ctgtttcttg aacagaggag gccttcatgg acactaaaaa agtggtaaac gaaagttggt
     3481 caaagaactc gacctcgcgg ctggcagctt ccacaagagc gtgagcacgc gcttgattga
     3541 tatccttacc tgcgtttata gcatcgttaa cacacgcttg aagatcttta gggagagagc
     3601 ctgagttcac ccctatacgc agggctgtat gagtatcctg ggcttttttc actacctctg
     3661 caattcgatc ctcactgccg atattacctg gattgatcct tattttagcg acgtttccat
     3721 ccatacaagc aagagcaaga cgccaatcaa agtgaatatc agcgaccaga ggcatggaag
     3781 taacttcagc taactggcac aaaaggcccg cactggctga atcgggaaca gcaaagcgaa
     3841 caatatcgca gccaagctgt tcaagcaggg atagtttgcg ggcaatgtcg tggatatccg
     3901 caccgacaag tggacttttc cacattgttt gtatggaaac aggcgcatct gcgccaatag
     3961 aaagggccct ggtttttccc tttccgccaa tagtaataag ccgggatttt agaaaatcac
     4021 tcatacgtac tcactatatc ggcaaaaaaa ggcgctgtac agaggcgaaa aaaaaccgtt
     4081 ccccgttttt ttacggagaa cggcacacat tcagtttaca atagatcgag ttgattacac
     4141 cgaaattaaa ccaaaaacca tgacgaacaa tgcaacggtt tcgcacaaac caacgatggt
     4201 gatgtattgg gaaaaacctt ttccggtttc tccaagagca tcggcaccgg ctgcagaagc
     4261 ggttccctga gcaatagccg aagaagcgat ggctgcacca cataccaacc caactccaag
     4321 aaggtaccag gggtcctttt tcgaaagaag catggcattc ataagaagaa aaccataaat
     4381 cgtctgggta agaggagcac cggcaaaggc aacaaggata aagggagcag gtttattgtt
     4441 catatagcac cgcttccagg aacctacggt cgcctgcccc gcaatggcaa gaccaatcgc
     4501 cgaaccaatc gcagaaatac cgagagcaca t
//
LOCUS       DAOQXQ010000040         4514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19591_length_4514_cov_2.616730, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000040 DAOQXQ010000000
VERSION     DAOQXQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19591_length_4514_cov_2.616730"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2643
                     /gene="dnaE"
                     /locus_tag="PKH81_01205"
     CDS             <1..2643
                     /gene="dnaE"
                     /locus_tag="PKH81_01205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNQ96684.1"
                     /translation="VRIARMCNVTIPQPGPVLPDYQIPSEFTTKEDYITHLVNEGLRQ
                     RYATVTAEIQERADFELSVIKKMDFVGYFLIVWDFIHWAKERNIPVGPGRGSGAGSIV
                     AYALQITDIDPLRYKLLFERFLNPERVSMPDFDVDFCFERRQEVIDYVRDKYGEDHVG
                     QIITFGTLKPKAAIKDVGRVLEIPLPEINALTKLVPDIPKAHIKDAFEVNESIQGSGQ
                     LAPYKDDPRYAELFEIAAALEGANRNTSLHACGMVIGKEVLTHYVPVYKDSKTGKIAT
                     QYTMDLIEDCGLVKMDFLGLKTLTLITHTEQLIRKRGGQWSAFSIAGISDTDRTTFKM
                     LGEGKSAAIFQFESQGMQNILKRAKPDKMEDLIALNALYRPGPMAYIDQFIESKWDPS
                     KIVYPDPCLKDILEETYGVIVYQEQVMQVAQRIAGYSLGQADLLRRAMGKKKPEILAK
                     EKIPFIEGAVKQGFNAEHAAEIYEILAPFAGYGFNKSHAAAYSVVAYQTAYLKANFPA
                     EFIAANLTNEINSTDKLPEYIAEGRKMGIPIDPPDINRSEAFFTVVEGHIVYGLLGIK
                     GLGEQAAHAIVSEREKNGAYTSFMDFLERLDLHTVNKRACEVLVQAGAFDLLGQTRST
                     LLANLERSIEYASHRKDSTRFGQVSLFEDSGVQEFTEFVFEELPDWPQQEKLRSEKEL
                     LGFYISGHPLDAYRKAFERAATFDIRYPERAQKDKQYTIVGMIRSIRPYQTKAGKWMA
                     FASFEDFNGIFDLTFFSSTWEKFRDKALPDSVWGFIGKVDTSRDTPGFLVDSLIDPNE
                     LQEKSVREVHIRIDPAVQEEKKIHPLRDFLFESSGNCSVYFHMDGLHKPYTIKAANQI
                     QVQANEDVLQRILELPGVLQVWKE"
     gene            2668..3258
                     /locus_tag="PKH81_01210"
     CDS             2668..3258
                     /locus_tag="PKH81_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNQ96685.1"
                     /translation="MLHSLFYTISSLISLYSLACFIRIIMTWIPTLEHSAAGRFISRL
                     CDPYLYWFRRFRFTRVGAIDFSPLLALGLLSVASMTFSTLAASGSITVGIVLAGLVQV
                     MWSIVSFMLNLFILFLIIRLILDLVNKYGYSPFVAAVDRLLNPLIARASRFLFRSRII
                     SYRASLLITLGMAVITRVGFGFGINALHQILVNLPF"
     gene            3262..>3940
                     /locus_tag="PKH81_01215"
     CDS             3262..>3940
                     /locus_tag="PKH81_01215"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNQ96686.1"
                     /translation="MYAGEIPIPVSRLKGVGPQTAKVLSNLDILTVGDLLSWWPRDWE
                     DRTVSTPLSRFAERKKIHTIARVASHDWFGAGRMKTLKLIIEDDEGTLAELICFNRPF
                     LEKQFPVDSRVSVYGSFRIAYGSIQSSAFEIDLDSQFSRKVFPVYPLTASLTQIQMRK
                     IISSALDSYAKGIDSELPQEVRSAHGIPAKHEILHMMHRPHRVEEATTAKEALIFEEL
                     YLFELAIG"
     assembly_gap    3941..3950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3969..>4514
                     /locus_tag="PKH81_01220"
     CDS             3969..>4514
                     /locus_tag="PKH81_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ96687.1"
                     /translation="MLDSDAQSNPLLPLQQRLVERLPFSLTDDQVNCILEINADITGE
                     TPMARLLQGDVGSGKTLVSFMAALAVIEQGGQVALLAPTELLARQHAEGAATLLEVLG
                     VRLAYLTGNVRASGRKELLTRLASGEIDVIVGTHALFSQKVQYKNLRLAIIDEQHRFG
                     VRQRAAIQEKGMEGGNGIPHLL"
BASE COUNT         1203 a         1111 c         1010 g         1180 t
ORIGIN      
        1 gtccgtatag cccgtatgtg caatgttacc attccacaac caggtccggt cctccctgac
       61 tatcaaatac cttccgagtt taccaccaaa gaagactata tcactcacct ggttaacgaa
      121 ggacttcgcc agcgatacgc aaccgtaacg gccgagattc aggaacgcgc ggattttgaa
      181 ttatccgtca ttaaaaaaat ggactttgtg gggtacttcc ttattgtatg ggattttatc
      241 cattgggcaa aagagcgcaa tattcccgtc ggacccggtc gtggatcagg ggccggatca
      301 atcgtcgcct acgcgctcca gattaccgac atcgatccct tacgatataa attgcttttc
      361 gagcgattcc ttaacccgga acgggtttcc atgcctgact ttgacgtaga cttttgcttt
      421 gaacgcagac aggaggttat cgattatgtc cgtgacaaat atggagaaga ccatgttgga
      481 caaatcatta cctttggaac gctcaaacca aaggcggcca taaaggatgt tgggcgagta
      541 ttggaaattc cccttcccga gataaacgca ctgacaaaac tggtgccgga tattcccaag
      601 gcacacatca aagacgcctt tgaggttaac gaaagcattc agggatcggg acagcttgcc
      661 ccctataaag atgatcctag atacgccgaa ctttttgaaa tagccgccgc cctggaaggc
      721 gcaaaccgga acacgagtct ccacgcatgc ggtatggtta ttggcaaaga agtccttacc
      781 cactatgtcc cggtatataa ggactctaaa acagggaaga ttgccaccca gtacaccatg
      841 gaccttatcg aagattgcgg tttggtcaag atggacttct tgggtttaaa aacccttaca
      901 ctaatcaccc ataccgaaca gcttatccga aaacgaggcg gtcaatggtc cgccttttcc
      961 atagcgggta tcagcgacac cgatcgtact acgttcaaaa tgcttgggga aggaaagagc
     1021 gcggctatct tccaatttga aagtcagggg atgcaaaaca tccttaaaag ggcaaagccc
     1081 gacaaaatgg aagaccttat tgcgttgaat gccctctatc gtccaggccc tatggcctac
     1141 atcgatcagt tcattgaatc aaaatgggat ccctccaaaa tcgtgtatcc tgacccctgc
     1201 cttaaagaca tccttgaaga aacctatggc gttatcgtgt atcaggaaca ggtaatgcag
     1261 gtagcccaga gaatagccgg ctactccctc ggtcaagcag atttactcag acgcgctatg
     1321 ggtaaaaaaa agcccgagat tcttgcaaag gaaaaaatcc cgttcatcga aggagcggtc
     1381 aagcagggtt ttaatgccga acatgccgca gagatatacg aaattcttgc accctttgca
     1441 ggttatggtt ttaataaaag ccatgccgcc gcatactcgg ttgtcgccta tcaaaccgca
     1501 taccttaaag ccaatttccc tgctgagttt attgcggcga accttaccaa cgaaataaac
     1561 tcgactgaca agcttccgga atacatcgca gaaggacgaa agatgggtat tcccatcgat
     1621 cctcccgaca ttaaccgctc cgaagccttt tttaccgttg tggaaggtca cattgtgtat
     1681 ggcttgttgg gtataaaagg gcttggtgag caggcggcgc atgcaattgt ttctgagcgt
     1741 gaaaaaaatg gtgcttacac atcatttatg gacttcctgg aacgactcga tttacacacg
     1801 gtaaataaac gcgcttgtga agtattagta caggccggag ccttcgatct actcggtcaa
     1861 accagatcta ccttgctcgc aaacctagaa cgctctattg aatatgcctc gcacagaaag
     1921 gattcaaccc gctttgggca ggtaagcctt tttgaagact caggggttca ggagttcacc
     1981 gaatttgttt ttgaagaatt gcctgattgg ccccaacaag aaaaactgcg ctctgagaaa
     2041 gagctgctcg gtttttatat ttctggtcac cccctggacg cataccgaaa agcctttgag
     2101 cgcgctgcaa ccttcgacat tcgctatccc gagcgtgcgc aaaaagacaa gcaatatacc
     2161 attgtgggca tgatacgaag catcaggccc taccaaacca aagccggaaa gtggatggcg
     2221 tttgcctcgt tcgaggattt taacggaata tttgacctca ccttcttttc aagtacctgg
     2281 gaaaaatttc gggacaaggc cttgcccgat tctgtatggg gctttatcgg gaaggttgat
     2341 acctcacgag atacccccgg attcctggtg gattctttaa tcgaccccaa tgaactacaa
     2401 gagaaatcgg tgcgggaagt tcacatacgc atagatccgg cggttcaaga agaaaagaaa
     2461 attcatccac ttcgtgactt tttgtttgaa tcatccggta attgttcagt atatttccat
     2521 atggatgggc ttcataagcc atatacaatt aaggcagcca accagataca ggtacaggca
     2581 aacgaagatg tgcttcaacg catacttgag ttacctggag tactacaagt ctggaaggaa
     2641 tagaccaata ctcaaaggat taccatgatg cttcattccc tgttttacac cataagttca
     2701 cttatttccc tctactcctt ggcgtgtttt atcaggataa ttatgacctg gattccaaca
     2761 ctggagcatt cggctgcggg aaggtttatt tcccgattgt gcgatcctta tctgtactgg
     2821 ttccgccgtt ttcgctttac gcgagtggga gcaattgact tctctcccct gttagccctt
     2881 ggcttactct ctgtggcttc catgactttt tccacgcttg cggcctcagg atcaataact
     2941 gttggaatag tacttgccgg ccttgttcag gttatgtggt ctatcgtcag ttttatgctc
     3001 aatcttttta tcctgtttct tatcatccgc cttattttgg atcttgtaaa taaatatgga
     3061 tattcaccct ttgtagcggc agtggaccgc ttgcttaatc cacttatcgc acgcgcatca
     3121 cggtttcttt tccgttcgcg catcatctct taccgcgcgt cccttcttat tacactgggc
     3181 atggcagtta ttacgcgcgt tggttttggt ttcggaataa acgctctgca ccaaatcctt
     3241 gtcaatcttc ccttttagcc aatgtacgcg ggcgaaatac caataccagt atccaggctc
     3301 aaaggtgttg gaccgcaaac cgcaaaagta ctttccaatc ttgacatctt aaccgtagga
     3361 gaccttctgt cctggtggcc cagagattgg gaagacagga ccgtatctac cccgctttcc
     3421 cgctttgcag agcgaaaaaa aatacacaca atcgcacggg ttgcctctca tgattggttt
     3481 ggagccggaa ggatgaaaac ccttaagctg ataatagaag atgatgaagg aacgcttgcg
     3541 gaacttatat gctttaatcg tccctttctg gaaaagcaat tcccggttga ttcacgggtt
     3601 tcagtatatg gctcttttcg tatcgcgtat ggatcaattc aatcttctgc gttcgagatt
     3661 gatctcgatt cccagttctc tcgtaaggta tttccggtat atcccctaac cgcttccctt
     3721 acccagattc aaatgcgcaa gataatcagc agtgcgcttg actcttacgc gaaaggaatt
     3781 gattcagaac ttccgcaaga agttcgtagc gcccacggga ttcctgctaa acacgaaatt
     3841 cttcatatga tgcatcgtcc acatcgagta gaagaagcca ctacagcaaa agaagccctg
     3901 atttttgagg agctctatct ttttgaactt gctattggcc nnnnnnnnnn cagtcaaccg
     3961 aaagagaaat gttggattcc gatgcacaat caaatcccct gcttcccctt caacagagac
     4021 ttgtcgagcg ccttcccttt tccctgacag atgaccaagt caactgtatt ttggaaatta
     4081 acgcagacat cacaggagaa actccaatgg cccgcctcct ccagggagat gttggctccg
     4141 gaaaaacctt agtctcgttt atggctgctc ttgcggttat tgagcaagga ggtcaggtag
     4201 cgcttcttgc accaactgaa cttttagccc gtcaacacgc cgaaggggcc gcgactctgc
     4261 tagaggtatt aggagtacgt cttgcctatc ttaccggcaa cgttcgcgca tcagggcgaa
     4321 aagaattgtt aacccggctc gcttccggtg aaatcgatgt aatcgttgga acacatgcct
     4381 tattctctca aaaagtacag tataaaaatc ttagactcgc catcatcgat gagcaacacc
     4441 gatttggagt ccgccagaga gcggcgatcc aggaaaaagg gatggaagga ggcaatggaa
     4501 tccctcatct gtta
//
LOCUS       DAOQXQ010000041         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19746_length_4485_cov_3.079684, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000041 DAOQXQ010000000
VERSION     DAOQXQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19746_length_4485_cov_3.079684"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1451
                     /locus_tag="PKH81_01225"
     CDS             141..1451
                     /locus_tag="PKH81_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ96688.1"
                     /translation="MATHQHIHPLADELNAILAPCVAGRLLSDLGTRLYFPKGIIAQS
                     GEAKKLGKTANATIGMAVKKGEPVMLTSLKEQLPSLTNAEAVAYAPTAGNDELRAKWL
                     EEIRKKNPSLGSLEVSLPVLVPGLTAGISYLSDLFLGDDSILLTADPAWDNYTLVVEA
                     RRNAILKQFTLFTETGFDRDGFKKAVIAEAKTGKVRMLLNFPQNPSGYTPTREEAADI
                     VTIVLEAAESGADVMVWCDDAYFGLDYEDNIESESLFARFANAHERILAIKIDGPTKE
                     DYVWGLRTGFLTFASKGMTTAQYEAIIKKLMGAIRSSVSCAATPSQSLMLKMLSDPRN
                     EAEKLAFRNMLEERYRLVRAFIDARPNNPVLKALPFNSGYFMSMKCIGIGAEELRLKL
                     LHESGIGTIAIDPSHLRVAFSSVDTDKIESVFSIIFKTAEELAK"
     gene            complement(1495..1959)
                     /locus_tag="PKH81_01230"
     CDS             complement(1495..1959)
                     /locus_tag="PKH81_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96689.1"
                     /translation="MVSEMIIKLSDHFNTNIVTRFLRPLLAGILADNEISRRISELTD
                     HPEAYTSQGIHVDELYLQILAMARFIYEVRTEVLPNLRLLSGGSGMHDSNKVYRDMAM
                     SNFGSNLQVLADYVHELYLKTVEYDKSHSGRDRPVYRDIPGLEEIGRYLIDK"
     gene            2041..3294
                     /locus_tag="PKH81_01235"
     CDS             2041..3294
                     /locus_tag="PKH81_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ96690.1"
                     /translation="MRTTFLAPFLALSLIMTTISGCSPSNKHIAVMWTNQSEFASYVE
                     LFNASQNRHRIVVEYKENPAGELITSKKGPDLVVGPWLKGEKARSRLIPTDYLFTELR
                     LNSRHFYSALLDLGNIRGRQYLLPVSFNLPSLIFSTENRQLIENDFFLSLDQVQRLSR
                     TFNAQTGGVYAKMGFSPRWNSEFLYLTARMFNARFEEGDSLVSWNKTGLTEATAYIQE
                     WTKMNGSAQAEADFQFKYLYDPPYRIVTGGRSLFAYIPSDELLILPQDKLANIDFRWI
                     VRDNTTPVRDDIIYMGICRNATGLDAAEAFIIWFYHEENQRRLLERSRSMGTMNHSFG
                     ISGGFSAIRAVNERTYPRFYPALLGHLPPPDSLGIPRILPNNWKTLKSEIIIPALEEA
                     VLPQSPATKADTILSDRLALWKKAQ"
     gene            3349..3741
                     /locus_tag="PKH81_01240"
     CDS             3349..3741
                     /locus_tag="PKH81_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96691.1"
                     /translation="MLSNIGPLRTMSYTMMSSVSASGRTYIPVKPSQYVYSQFQHVAG
                     VPARNGQQGVSVDKIKILNTLIDQLIQLKQKNSEQKHTTTGDMSDGQINALISQYQDQ
                     VRGAVAIAEQMPYKPAMPQTGAVINLVA"
     gene            3893..>4485
                     /locus_tag="PKH81_01245"
     CDS             3893..>4485
                     /locus_tag="PKH81_01245"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944825.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNQ96692.1"
                     /translation="MTINDIKHPKVKAWVEEVAKMCQPDSVYVCDGSQKEYDTLMKKL
                     IDCGMATPLKKRPNSVLFRSQPSDVARVENRTYIASKTKEAAGPTNNWIDPNELKKTM
                     TGLYTGCMKGRTMYVIPFSMGPVGSDIAKIGIEITDSEYVVCNMDIMTRVGTKVLDVL
                     GTDGEYVPCLHSVGKPLAAGESDNGQWPCADIENKYIS"
BASE COUNT         1236 a         1220 c          958 g         1071 t
ORIGIN      
        1 taagtatcga ttcaactcgt tcatgccctt ctacgcgctc tttcatggta tacctgccag
       61 tattttcagc gtttttttca ttctacgcgc cttggacgag attgacaacc ggttcccgca
      121 cgttgtatga gatagttact atggcaaccc accaacatat ccatcccctt gcggatgaac
      181 taaacgcgat tctcgccccc tgtgtagccg gacggctttt atccgatctc ggcacccgcc
      241 tgtatttccc caagggcatc atagcccaaa gcggcgaagc caaaaaactt ggcaaaacgg
      301 caaatgccac tatcggcatg gcggtaaaaa aaggtgaacc ggtaatgctt acctcgctca
      361 aggagcagct tccttctctt acaaacgcgg aagcggttgc gtacgcgcca acagcgggta
      421 acgatgagtt acgcgcaaaa tggctggaag aaattcgcaa aaaaaaccct tccctcggct
      481 cccttgaagt ttccttgccg gtcttggtac caggccttac agccggaata tcctatctgt
      541 cggacctctt ccttggcgac gacagcatcc tccttaccgc agaccctgcc tgggataact
      601 acaccctggt ggtagaagcg cggagaaacg ccatccttaa acaatttacc ctctttaccg
      661 aaaccgggtt tgaccgcgac ggctttaaga aagcagtcat agccgaggcc aagacaggca
      721 aggtgcggat gttgcttaac ttcccccaaa acccctcggg ctacacccct acccgcgaag
      781 aggcagcgga catcgtaacc atcgttttag aggccgcaga atctggagcc gacgtaatgg
      841 tatggtgcga cgatgcgtac tttggccttg attatgaaga caacatagaa agcgaatccc
      901 tctttgcccg ctttgccaac gcgcacgaga ggatccttgc cattaaaatt gatggaccca
      961 ccaaagaaga ttatgtatgg ggccttcgca cgggattcct tacctttgca tcaaaaggga
     1021 tgacgacagc ccagtatgaa gcgatcatta aaaagctcat gggggccatc cgctcctcag
     1081 tttcctgcgc ggcgacccca tctcagtctt taatgctcaa gatgctcagc gatccccgta
     1141 acgaggcaga gaaactcgca ttccgcaaca tgctcgaaga gcgataccgc cttgtcaggg
     1201 cctttattga cgcgcgaccg aacaatcccg tgctcaaagc cctacccttt aactcaggat
     1261 attttatgag tatgaagtgt atcggtatcg gtgccgagga attacggctt aagctgctgc
     1321 acgaaagtgg tatcggaacc attgccattg atccctcgca tctgagggtt gccttttcat
     1381 ccgtagatac cgacaagatt gaatcggttt tctcgattat ctttaaaaca gccgaagaac
     1441 tggcgaaata accagcagtc aacaaaaagg cggttcccag ggaaccgcct ttttttattt
     1501 atctatcaag tagcgaccaa tctcttccag gccgggaata tcccgataga ccggacgatc
     1561 tctgccacta tgactcttgt cgtattcaac cgtttttagg tagagctcat gtacgtaatc
     1621 ggccaatacc tgcaggtttg acccaaaatt actcattgcc atatcccgat ataccttgtt
     1681 tgaatcatgc atccccgagc cacccgataa taagcgcaaa ttgggcaaca cctcggttcg
     1741 tacctcataa ataaaacggg ccatagccaa aatttgtaaa tatagctcgt caacatgaat
     1801 cccctgagag gtatacgcct caggatgatc agttaactcc gaaattctcc gcgagatttc
     1861 attatccgcc aaaatgcctg caagcaaggg cctaagaaat cgtgtcacta tattggtatt
     1921 aaaatgatcg gaaagcttaa taatcatttc cgataccatt ggatcccgta taaccatacc
     1981 ccagtatacc ccgagcaatc gggattgaca acaggaaggc ctatctctag agtattctcc
     2041 atgagaacga ctttcttggc accatttctt gccctatccc tgataatgac tactatttcc
     2101 ggctgttctc cctcaaacaa gcatatagcc gtcatgtgga ctaaccaaag cgaattcgcc
     2161 tcctatgttg aattatttaa cgcaagccaa aaccgccacc gaatcgtcgt tgaatataaa
     2221 gagaaccccg caggggagtt gataacttcc aaaaaaggtc ctgatcttgt tgtaggaccc
     2281 tggctcaagg gagaaaaagc ccgttcacgc ctcataccaa ccgactacct ctttacagaa
     2341 ctcagactca attcacgaca cttttatagc gcattgcttg atttgggaaa tatccggggg
     2401 cgccagtatc ttcttccggt cagctttaac ctcccgtcac tcattttttc cacagaaaac
     2461 cgtcagctta tcgaaaacga cttttttctc tccctggatc aagtgcaacg tctttcgcga
     2521 acctttaacg cacaaactgg cggagtctat gccaaaatgg gattttcacc cagatggaat
     2581 agtgaattcc tctatttgac cgcacgtatg ttcaacgccc gctttgaaga aggagactcc
     2641 ctggttagct ggaataaaac cggtcttaca gaagcaacgg cgtacataca agaatggacc
     2701 aagatgaacg gttccgccca ggcagaagcc gatttccagt tcaagtacct gtatgacccg
     2761 ccctatcgaa tcgttaccgg aggacgaagt ctctttgcgt atattccaag cgacgagtta
     2821 ttgatcctgc cgcaggacaa actggcaaat attgacttta gatggattgt cagggataac
     2881 accacaccgg tgagagatga cattatctat atgggtattt gtcgcaacgc taccggcctt
     2941 gatgccgcag aagcctttat tatctggttt tatcatgaag aaaaccaacg ccgccttctg
     3001 gagcgaagca gaagcatggg caccatgaat cattcctttg gcatttccgg tggattttct
     3061 gccatcagag ccgttaacga gcgaacctac ccgcggtttt accccgcgct ccttggtcat
     3121 cttcccccac cagattccct tggtatacca cggattctcc ccaataactg gaagaccctc
     3181 aagtccgaaa taataatccc cgctctggaa gaagcagtcc ttcctcagtc cccggcgaca
     3241 aaggccgaca ccattctttc ggatcgcctt gccttgtgga aaaaggccca gtaaaaaaaa
     3301 gatacatttt cctccatttc ttccgatagt aacaatggag gagatacaat gctgtccaat
     3361 atcggtccac tcaggaccat gtcatacaca atgatgagct cggtttcggc cagtggcagg
     3421 acctatatcc cggtcaagcc aagccaatat gtgtattcac agtttcagca tgtggcaggc
     3481 gtccccgccc gaaacggcca gcaaggcgtt tctgtagata aaatcaagat acttaatacc
     3541 cttatcgatc aactcatcca acttaaacaa aaaaattccg aacaaaagca caccactact
     3601 ggcgacatga gcgatggaca aattaacgcc cttatttctc aatatcaaga tcaggtcagg
     3661 ggcgctgtcg ctattgcaga gcaaatgccc tataaaccgg ccatgcccca aaccggagca
     3721 gtcattaacc tcgtagcttg atcaatattg caccaacaat cctgctaaca gtgcttaaga
     3781 tttgtcaact cacccttaca tatcttgcat cttttacgct aatccgctat tgtttccttg
     3841 agtgaagcga aaaaaaaagt tcttcactat tcaagcaatg caaggagcaa gtatgacgat
     3901 caatgacatc aagcacccca aggtaaaagc gtgggtcgaa gaagtcgcca agatgtgcca
     3961 gccggacagc gtatacgtct gtgacggttc ccagaaagaa tatgataccc tgatgaaaaa
     4021 gctcatcgat tgcggtatgg caacccccct caaaaaacgc cccaacagcg ttttgttccg
     4081 ctcccagccc tcagacgttg cacgcgtaga aaaccgcacc tatatagctt caaagacgaa
     4141 ggaagctgcg ggtcccacca acaactggat cgatcctaac gaactcaaga aaaccatgac
     4201 cggactctac accggctgca tgaagggccg cacgatgtac gtcatcccct tctccatggg
     4261 tcctgttggc tccgacattg ccaagattgg tattgaaatc actgactctg aatacgttgt
     4321 ctgcaacatg gatatcatga cccgtgtagg aaccaaggtt cttgacgtac tcgggactga
     4381 cggtgaatac gtcccctgtc tccactcggt aggaaaacct ctcgccgctg gcgaaagcga
     4441 taacggtcaa tggccatgcg cagatatcga aaacaagtat atatc
//
LOCUS       DAOQXQ010000042         4471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_19841_length_4471_cov_3.761096, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000042 DAOQXQ010000000
VERSION     DAOQXQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19841_length_4471_cov_3.761096"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PKH81_01250"
     CDS             complement(<1..817)
                     /locus_tag="PKH81_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ96693.1"
                     /translation="MPRKVHIRTKIFFIVLVAVLPTVLFSVYTTLSFRSAYLEGSATY
                     LTNLCNSFTNEQRLIARNAEQMLLAISKTRSVMNKEYSFLNRYLSDLMFLYQDYAVLL
                     CANSDGMVIASSINKTGYSLSDRPYFNHAKATNAFTIGQYIVSRSTGVSSITYTLPVR
                     DTSGSRVFLIGTYALDKYSRELSLGRLGKDAVLEIADSDGVCLFSSSSDPLIVPGQPL
                     PSSLFSLMKDHARKNPKKVEIEGKEWFASTDYYSRNGTALYISVRIPYENVVRE"
     gene            877..2433
                     /locus_tag="PKH81_01255"
     CDS             877..2433
                     /locus_tag="PKH81_01255"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ96694.1"
                     /translation="MDVQSIIKNLHPLEIRVLRQYGPNTELTMERLEKDLGYKTGHAN
                     QAFSWLGGKELVREIRRESNTFFEITDLGRKYCSEGTADEKILKLLQGSASLSLPEIA
                     TALDLEQKDIGSAFGILSKEGAVTMDAQKKASFVSIPTTPRYTSTRILLEKAVATEGG
                     LLNQKDLSSDEATVMGTLAKKRGASDSPFRVLERENVVYTLTESAALIKKALDDAGIT
                     GDEIGSITPSLLKNGQWKTGSFRGYNTAIPPARIIPGRTSPYVSFLESVKDKLCSLGF
                     EEFDGPLVETEFWNSDALFMPQFHAARDIHDAYYLKNPTHAKEIEEPWLSNVAAAHQN
                     GGNTGSRGWNYSFDREFTRRLILRSQGTALSAHQLHKAKVPGKYFGIARCFRYDKVDA
                     THLSDFYQTEGIVLGNEVNLRTLLGFLEMFAIEIAGATEVKYVPGYFPFTEPSIEVHI
                     KHPVLGWFELGGSGIFRPEVTRAMGIDVPVLAWGIGIDRMALMALGLNDLRELFSSDI
                     EGVRLRKAKY"
     gene            2445..4178
                     /gene="pheT"
                     /locus_tag="PKH81_01260"
     CDS             2445..4178
                     /gene="pheT"
                     /locus_tag="PKH81_01260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ96695.1"
                     /translation="MPKIEVNEKLFFKLLGKTYDYHTLEDRLTCAKAELDEQPDPSLP
                     ESDRIIKIELNDTNRPDLWSTAGIARQLRLHYQGLSAEKIKYKDFFSQPGMSRDAAQR
                     IIKVDPRLKDIRPYMSAFVISGKPIDEPMLLDIIQTQEKLCWNYGRKRRSISMGIYRS
                     SAISWPVQYTAVDPDTTSFVPLGCDAPMTCRQILTDHPKGKEYGWILKDKQLFPLLID
                     AKKEVLSMAPIINSATLGAVQVGDSDLLVEMTGTDMPTLTLATSIVACDFADAGYTIL
                     PCKIEHPYDTGFGNEITTPYYFQESTTTTVGDINRLLGVDLSINEIITALDKMGCSTK
                     ITGEQITVSPPEYRNDFLHGVDIIEDVMMGREISSFPAERPCEFTIGRLSPVTIFSRK
                     AKSLMVGLGYQEMIFNYLGSRKDYIERMNIAGTEEENKVIEISNPMTENFQFVRPSIL
                     ASLLAAESPSGNAVYPHRIFEIGKVAYLCQEENTGTRTRQQLGFLTASSDANFNEAAS
                     QVASLLYYLDHEYEVKDSTDPRFIPGRQAFILVNGKKVGIFGEIHPQVLENWTIGMPC
                     VAAELDLEELM"
     gene            complement(4219..>4471)
                     /locus_tag="PKH81_01265"
     CDS             complement(4219..>4471)
                     /locus_tag="PKH81_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521089.1"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HNQ96696.1"
                     /translation="PISSLKSYMGHTLGACGAIEAWLSIKMMREGWFAPNLNLENIDQ
                     ECGKLDYIQGHGRTIDTEFIMSNNFAFGGINSSLIFRRI"
BASE COUNT         1332 a         1031 c          929 g         1179 t
ORIGIN      
        1 cttcccgaac tacattttca tacgggattc gaacggaaat atataaggcg gttccgtttc
       61 tggaataata atccgtggat gcaaaccatt ctttgccttc tatttcaacc ttcttgggat
      121 ttttccgtgc atgatctttc atcagactaa acaatgacga aggcaacggt tgtccgggaa
      181 caataagggg atcgctactg ctagaaaaaa ggcacactcc atcgctatca gcgatctcca
      241 gaaccgcgtc ttttcccaaa cgacccaaag aaagctctcg ggagtattta tcaagagcat
      301 aagtaccaat caagaataca cgagaacctg aagtatctct taccggaaga gtgtaggtta
      361 tggaagagac ccctgttgat ctactcacaa tatactgacc gatagtaaag gcattcgttg
      421 cctttgcatg gttaaaatag gggcgatccg aaagcgagta tcctgtttta tttatgctgg
      481 aagctattac catcccatcc gaattggcac aaagcaaaac tgcgtaatct tggtacaaaa
      541 acatcaaatc acttagatac cgattcaaaa aagaatattc tttattcatt actgaacgtg
      601 ttttagagat ggcaagcaac atttgttcgg catttcgtgc gatcaaacgt tgttcattag
      661 taaagctgtt acaaagattg gttaaatagg tcgcgcttcc ttcaagatac gccgatctga
      721 acgaaagggt tgtatataca gaaaacagca cggtgggtaa taccgcgaca agaacaatga
      781 agaaaatctt tgtgcggata tgaacttttc tcggcaccct ttactgcact ccaaagaaaa
      841 tggtataaat attcattatt gtatcaggag ttactcatgg atgtacagtc aattatcaag
      901 aatctgcatc cgctcgaaat acgcgttctt agacagtacg gtccaaacac cgaactgacg
      961 atggagcgcc tcgagaagga tctcggctat aagaccggac acgcgaatca agctttttca
     1021 tggcttggag gcaaagaact agtacgcgaa attcgtcgcg aaagcaatac tttttttgaa
     1081 attaccgatt tagggcgaaa atattgttcc gagggaactg ccgacgaaaa aatccttaaa
     1141 ctcctacagg gttcagcttc gctaagcctg cccgaaattg ctaccgccct ggaccttgag
     1201 caaaaagaca tcggctctgc ttttggaatt ctttccaaag agggtgccgt taccatggac
     1261 gctcaaaaaa aggcctcttt tgtgtcaatc ccgacaacac ctcgatacac atcaaccaga
     1321 atcctgcttg aaaaagctgt ggctacagaa ggggggttgt tgaatcaaaa agacctctct
     1381 tccgacgaag ctactgtaat gggaaccctt gcaaaaaagc gcggtgcttc tgactccccc
     1441 ttccgggtgt tggaacgtga aaacgttgta tacacgctta ccgagagcgc tgctttgatt
     1501 aaaaaagccc ttgatgacgc cggtatcacc ggagatgaga tcggttcaat aaccccgagt
     1561 cttcttaaaa acggtcaatg gaaaacaggt tcctttagag gttataacac cgccattccg
     1621 ccagcacgta ttattcctgg ccgcaccagt ccctacgtaa gctttttaga aagtgttaag
     1681 gacaagctct gttccttggg ttttgaagag tttgatggcc cgcttgtaga aactgaattc
     1741 tggaattcag atgccctgtt tatgccacaa ttccatgccg cccgtgatat ccatgacgcg
     1801 tattatctaa agaatccaac acatgctaag gaaattgaag agccctggtt atcaaatgta
     1861 gcggctgcac atcaaaatgg cggtaacacc ggaagtaggg gatggaacta ctcctttgac
     1921 cgtgaattta cccgtcgcct catccttcgc agccagggaa cagctttgtc ggcacatcaa
     1981 ttacacaaag caaaggttcc cggtaaatat tttggaatag cgcgctgctt ccgttacgat
     2041 aaggtcgatg ctacccacct gtccgatttt taccaaaccg agggaatcgt tcttggaaat
     2101 gaggtaaatc tcagaaccct ccttggcttc ctggaaatgt tcgctatcga aatagctggt
     2161 gccacagaag taaaatatgt ccccggatac ttccctttta cggaaccgtc aatagaggtt
     2221 catattaaac atccagtctt aggctggttc gaacttggcg gatcaggaat tttccggccc
     2281 gaagtaactc gcgccatggg tatagacgta cccgttttgg catggggcat tggaattgac
     2341 cgaatggcgc taatggctct tggcctaaat gatcttcgcg aactattcag ttccgacatc
     2401 gaaggcgtcc gcctcagaaa ggcaaaatac taaggagaac cactgtgcca aaaatagaag
     2461 tgaacgaaaa actgtttttc aagctactcg gtaaaacata cgattatcac acattagaag
     2521 atcgccttac ctgtgcaaag gcagaacttg atgaacaacc tgatccctcg ctcccagaaa
     2581 gtgatcgaat aatcaaaatc gagcttaacg acaccaatcg tccagacctt tggtcaacag
     2641 caggcatagc tcgtcaactc agactgcatt atcaaggatt aagcgccgaa aagataaaat
     2701 acaaagactt cttttcacaa cccggtatgt cccgtgatgc agcccaacgt attatcaagg
     2761 ttgatcccag actcaaggac attcgtccct acatgagcgc ttttgtaata tccggaaaac
     2821 caattgatga acccatgctt cttgatatta ttcaaactca agaaaagctc tgctggaatt
     2881 atggccgtaa acgaagatcc atctccatgg gtatttatag atcctctgct atttcttggc
     2941 ccgttcaata taccgcagtt gatcctgata ctaccagctt tgtacctctt ggctgcgacg
     3001 caccaatgac ctgtcgtcaa attcttactg accatccaaa aggtaaggaa tacggatgga
     3061 ttcttaagga caaacaactc ttcccgcttt taatcgatgc taaaaaagaa gtcctttcaa
     3121 tggctcccat tatcaacagc gcgacgctcg gcgcggttca ggtaggcgat agcgatttgc
     3181 ttgttgagat gacaggaaca gacatgccaa ccctcacgct tgcaaccagt atcgttgcct
     3241 gcgattttgc tgatgcagga tataccattc tcccctgcaa aatagaacat ccctatgata
     3301 ccgggtttgg taacgaaata acaacgccat actattttca ggaatctacg actactacag
     3361 ttggcgacat taaccgcctt cttggagtag atctgagcat taacgaaatc atcacggcct
     3421 tagataaaat ggggtgcagt acaaaaatta ccggtgaaca gataaccgta agccctcctg
     3481 aatatcgaaa tgatttcctt catggcgttg atattatcga ggatgttatg atgggacgtg
     3541 aaatcagctc ctttcctgca gagcggccct gcgaatttac cattggtaga ctttctccgg
     3601 taacgatttt tagccgtaag gctaaaagct tgatggtcgg cctgggatat caggaaatga
     3661 tctttaatta tctcggttca agaaaagatt atatagaacg tatgaatatt gctggtacag
     3721 aagaagaaaa caaggttatt gagatttcaa atccaatgac agaaaacttc caattcgtta
     3781 gaccttctat ccttgcaagt cttcttgctg ccgaatctcc atcaggaaat gctgtgtatc
     3841 cccatcgtat tttcgaaatc ggaaaggtcg cctatctatg ccaggaagaa aataccggaa
     3901 caagaacgcg ccaacaactt ggttttctta cagcttcatc cgacgcaaat tttaacgaag
     3961 cggcaagtca agttgcaagc cttttgtatt atcttgatca tgagtacgag gttaaagatt
     4021 caacagatcc tcgctttatt cccggccgcc aggcattcat tttagtaaac gggaaaaaag
     4081 ttggaatctt tggagaaatc caccctcagg tattggaaaa ctggacaatc ggtatgcctt
     4141 gtgtagcggc agaacttgat cttgaagagt taatgtaatg taatacatta aaaaccgccg
     4201 caatcgcggc ggtttttttt atatccttcg aaatataagc gaactattta ttccaccaaa
     4261 agcaaaatta ttactcataa taaactcagt atcgatagta cgcccatgtc cctgtatata
     4321 atccagttta ccgcattcct gatcaatatt ttccaaattt agatttggag caaaccaccc
     4381 ctctctcatc atcttgatag ataaccatgc ttcaatcgcg ccgcatgctc ccagggtatg
     4441 ccccatataa ctttttaaag agctaattgg c
//
LOCUS       DAOQXQ010000043         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20056_length_4440_cov_3.619384, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000043 DAOQXQ010000000
VERSION     DAOQXQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_4440_cov_3.619384"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKH81_01270"
     CDS             complement(<1..872)
                     /locus_tag="PKH81_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96697.1"
                     /translation="MSLADTWLLAWPQALAVWSKFTMLCDPILCTTRIQASAEGVSES
                     FAAIRFTDKRVVIDLESVKKKKLDKYAVEILAHEAGHHVYCPANQTANYRMLAKIHKA
                     LPGLQQHISSIANMYCDLLINDRLKRQASLRMDQVFIALGKAGSTSSPVWILYMRIYE
                     VLWKLDKGTLCDATLPSAMESDAWLGSRIIRVYAHDWLTGASRFAVLFARYLDQDEER
                     ESPGSFRIFLDLNNALSGCEPSGIVAIDKSDADILHPGEDPLLSGVYIPSVPVQMEEV
                     NEAGTGQAREPWEYG"
     gene            complement(869..1885)
                     /locus_tag="PKH81_01275"
     CDS             complement(869..1885)
                     /locus_tag="PKH81_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96698.1"
                     /translation="MSHLSSSSALACSLTKHKKLINERIAQARRLYLGLDMESFSTLL
                     TGPVAKVIDSLEDSIDECALTAVDTLLIMARHGFLISPYREILASLWTDVLAGCTALV
                     PSAPHQVIGRLHNVLLNMRSRSVDFFRAWIDSLAAVAPFASTKEELFALASVLIWKNG
                     NAAYRKSALAAAKTLPSALVLRVFNAADMSGQEVLNRLGKDRWYNPSLAEPVGFTNRW
                     IVGGFLGNGGPFIKPVSVRVGSDGFILSDGLQAHHLFVDAFGCEICPMRDVPERVYKK
                     SFLERAEEVSQSGRWIVHKKTKVPFYFMDVDAAITCDNDTVVAFSPWSYYLQVLSLPP
                     QAPV"
     gene            complement(1882..3483)
                     /locus_tag="PKH81_01280"
     CDS             complement(1882..3483)
                     /locus_tag="PKH81_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96699.1"
                     /translation="MSDEAKKTVFNPIGHISKSPTIPGKGKQYRDFDVAQLFEGPCTP
                     EANNEVAPSPLDEKLRQAYFWVVNTAIISPHYDVEYNEGPPTSMVVGDSKRSLTLPSA
                     QSYSSYVLLPLLTFAVRGKCLFIGGPGRGKTASALLMGVLAGYPVKEVKRSMQHGHPQ
                     MTIADLLGNPLPADLVNAKSMDDIQIAWRSWLSMRVKIIDEYNRIPTRTQSALLTVMG
                     DNYAEVLNHIYECPESAWYLTANDDKGGGTYEVVEALRDRIDVVVQALAFNPRFLEEL
                     LVRVENKIRPEEFVPSEIIFTQEEVDRMGDEIRSVAVPPDVLRRIEFFASQFEFFETA
                     GTQFEYMTKDTAHVSGAQWRDLVSADNGRDRLKDLGCQTMNGLSVRNLMTLLLYAKAV
                     AYFRGNKSVSLDDVRQILPFVLQDKLHADLDSPFFDLGENTAYKADRIGWLRYLFDSS
                     CQEFVRLNLDEDDPVRDVHNEFAKGLEGLSHSVAHKRLVNIEQLIRSRAKEGKLYGHV
                     YDDLLKLKYLHQRYTNYLNWLKEST"
     gene            complement(3483..4121)
                     /gene="pnuC"
                     /locus_tag="PKH81_01285"
     CDS             complement(3483..4121)
                     /gene="pnuC"
                     /locus_tag="PKH81_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNQ96700.1"
                     /translation="MERLLSVHTVCLVIGNYQLSWIELIGTLFYFASVFLISRRKIST
                     WPVGIVSVILYGILFYQIQLYSDMLEQGYYLVISVIGWMMWNKQKKTEGAVGSLWSSP
                     QGIGLAIVITLVGTSILAFCVSRFHLWLPSLFPEPASYPALDALTTVMSFVAMYLTTI
                     RRTEGWVYWIIVDVLAVWLYWVKDVRFISAQYVFLLCMAIYGLYFWSKRKSA"
     gene            complement(4106..>4440)
                     /locus_tag="PKH81_01290"
     CDS             complement(4106..>4440)
                     /locus_tag="PKH81_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96701.1"
                     /translation="STRDIAFIDTSAITTLAYAHYYFDRASTALEKIVEKNRWKYRHV
                     LLCGDEIPFEDTPDRSGPESRGRLQKINEQLLDKYKIPYTRVFGSREDRGILIQNYIL
                     KGDIWNGY"
BASE COUNT         1252 a         1123 c          972 g         1093 t
ORIGIN      
        1 ccatactccc agggctcgcg agcctgtccg gttccagctt cgttcacttc ttccatctgg
       61 acaggtacag aaggaatgta gacaccgctc aacaaggggt cttctcctgg atgcaagata
      121 tcggcatcgc tcttatcaat agccactatg ccagaaggtt cacatcctga taacgcgtta
      181 ttaagatcca aaaaaatcct gaaggaacca ggggattcac gctcctcgtc ttgatcaagg
      241 tagcgagcaa aaagcacagc aaatcgggag gcaccggtaa gccagtcatg tgcatagacg
      301 cgaataatgc gtgaacccag ccaggcatcg ctttccatgg cagacggtaa tgttgcatcg
      361 cacagagttc ctttatccaa cttccacaac acctcatata tccgcatata taatatccac
      421 acaggagatg aggtgctccc ggccttgccc aaagcaataa agacctgatc catacgcaat
      481 gaggcttgtc gtttaagcct gtcgtttata agtagatcgc aatacatatt tgcaatagaa
      541 gaaatgtgct gttgtaaacc aggaagcgcc ttatgaatct tggccagcat cctgtaatta
      601 gctgtttgat tcgcaggaca atacacatga tgccctgctt catgcgccag gatttccacc
      661 gcatatttgt ccagtttttt cttttttaca ctctcaaggt ctatcacgac tcgcttgtcg
      721 gtaaagcgaa tcgccgcaaa ggattcacta accccttcgg cagacgcctg gattcgcgta
      781 gtgcataaga ttgggtcaca gagcatggta aacttgctcc atacggccag agcctgtggc
      841 caggccaaaa gccatgtgtc ggcaagactc atacgggggc ctggggtggc agggaaagta
      901 cctgcaggta atacgaccaa ggagaaaaag cgacaacagt atcattatca caggtaattg
      961 ccgcgtccac atccataaaa taaaacggga ccttggtttt tttatgcact atccatctgc
     1021 cggattgcga cacctcttct gccctttcta aaaaactctt tttatagacg cgctccggaa
     1081 cgtcacgcat cggacagatc tcgcacccaa aggcatcaac aaataaatga tgagcttgta
     1141 atccatcact caggataaac ccgtcagatc caacacgtac ggacacaggc ttaataaatg
     1201 gccctccgtt acccaaaaag cctccgacaa tccagcggtt ggtaaagccc accggttccg
     1261 ccaaggatgg gttataccaa cgatccttgc ccagtcgatt caatacctcc tggcccgaca
     1321 tgtcagcggc attaaaaacg cgaagtacaa gtgcggaggg gagtgttttg gccgcagcaa
     1381 gggcactttt ccgatacgct gcattaccat tcttccagat taacacagaa gcaagcgcga
     1441 aaagctcttc ctttgtggaa gcgaatggtg ctaccgccgc taaggaatct atccatgcgc
     1501 gaaagaagtc aacgcttctg gagcgcatat taagaagcac attgtgcaag cgcccaatta
     1561 cctggtgcgg agcgcttggg acaagggccg tgcatcctgc taacacatca gtccataatg
     1621 aagccaggat ttccctgtat ggagaaatca ggaagccatg gcgagccatg atgagcaggg
     1681 tatccacagc agtcagggcg cattcatcaa tggaatcttc caaagaatca attaccttag
     1741 caacggggcc ggtcagcaaa gtgctaaagg attccatgtc aagaccaaga tacagacgcc
     1801 tcgcttgtgc tatccgttcg ttaatcagct tcttatgttt ggttaacgaa caggcaaggg
     1861 cggaacttga actaagatgg ctcatgtgct ttccttaagc caattaagat agttggtata
     1921 ccgttgatgt aaatacttca acttaagcag gtcgtcatac acatgtccgt agagtttccc
     1981 ctctttggcg cgagagcgaa tcagttgctc aatattgaca aggcgtttat gggctaccga
     2041 gtgactgagc ccttcaagac ccttggcaaa ctcattgtgt acatccctaa caggatcatc
     2101 ctcgtccagg ttaagacgaa caaactcctg gcaggagctg tcaaataaat acctgagcca
     2161 accaatgcgg tctgccttgt aagcggtgtt ttcgccaagg tcgaaaaaag gagaatccag
     2221 gtctgcatgt aatttatctt gcaatacaaa ggggagtatt tggcgcacat catccaaaga
     2281 caccgacttg tttccgcgaa aataggcgac ggcctttgca tatagcagaa gggtcatcag
     2341 gtttcgcaca gacagaccgt tcattgtttg acatcccaaa tccttaagcc ggtcacgtcc
     2401 attatccgcg cttaccaagt cccgccactg ggcacctgat acatgagcgg tatccttggt
     2461 catatattca aattgagtgc cggcggtttc aaaaaattca aattggctgg caaaaaattc
     2521 aattcgtcgc aagacatcgg gagggacggc cactgaacga atctcatcgc ccatacgatc
     2581 cacttcttcc tgggtaaaaa taatttcgga aggaacgaat tcctctggcc gaattttgtt
     2641 ttctacacgg accagaagct cctcaagaaa gcggggatta aaagccaagg cttgaacgac
     2701 cacatcaatg cgatcgcgca aggcttctac aacttcgtat gttcctccac ccttgtcgtc
     2761 gttcgcggta agataccagg cagactccgg gcactcgtaa atatggttta aaacctctgc
     2821 atagttatcg cccattacgg ttaacaatgc gctttgagtt cttgtgggga tgcggttata
     2881 ctcgtcgata atttttacgc gcatggaaag ccaggatctc cacgcgattt gaatatcatc
     2941 catgcttttg gcgtttacta aatccgccgg caagggattt cccaagagat cggcgatcgt
     3001 catttgcgga tgaccgtgtt gcatggatcg ttttacttct tttaccggat atcccgcaag
     3061 cacgcccatt aaaagagcgc tcgcggtttt tccgcggccc ggacctccaa taaaaaggca
     3121 cttaccacga actgcaaagg taagaagagg caacagaaca tagcttgaat aactttgtgc
     3181 agacggtaat gttaaagacc gctttgaatc cccaactacc atactcgttg gcggaccctc
     3241 attatattca acatcataat gaggactgat aatcgcggta ttaaccaccc aaaaatacgc
     3301 ttgtcttagt ttttcatcaa gcggagatgg agctacctcg ttatttgctt ccggggtaca
     3361 cgggccctca aataactgag ccacgtcaaa atccctatac tgcttaccct tccccggtat
     3421 ggtaggagat tttgaaatat gacctatcgg gttaaacacc gtcttctttg cttcatcact
     3481 cattatgcac tcttcctttt tgaccaaaaa tacaaaccat atattgccat acagagcaag
     3541 aaaacatatt gagcggagat aaatcgcaca tccttaaccc aatagagcca cacggcaagt
     3601 acgtcaacga taatccagta tacccatcct tcggttctcc gaatggtagt aagatacatg
     3661 gctacaaaac tcataactgt agtaagcgca tccaaggcgg gatacgaagc gggttcgggg
     3721 aaaagagagg gaagccacag atgaaagcgg gaaacacaga aagccagaat acttgtgccc
     3781 accagagtga tgactatcgc caggcctata ccttgtggac tactccacaa agagcctacc
     3841 gcgccctcgg tttttttctg tttgttccac atcatccatc caatcacgct gattaccagg
     3901 taatacccct gctcaagcat atcagagtac agttgaattt gataaaacaa tatcccgtac
     3961 agaatgaccg agactatccc aaccggccag gtactgattt ttcttcggga gatcaagaat
     4021 actgaggcaa aataaaaaag agtcccaatt aactcaatcc aacttagctg ataatttcca
     4081 ataactagac acacagtgtg tacgctcaat agccgttcca tatatcgcct tttagaatat
     4141 aattttgaat gagtatacca cgatcttccc tgctaccgaa tacccgcgta taaggaattt
     4201 tgtacttatc caggagctgt tcgtttattt tttgaagtct tccgcgagat tcagggcctg
     4261 agcggtccgg cgtgtcttca aacggaatct cgtcgccaca caataacaca tgacgatact
     4321 tccaccggtt tttctctact attttttcta gcgcagtact cgctcgatca aaataataat
     4381 gggcataggc cagggttgtt atcgccgagg tgtctataaa agcaatgtct cgcgtgctct
//
LOCUS       DAOQXQ010000044         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20218_length_4413_cov_2.868059, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000044 DAOQXQ010000000
VERSION     DAOQXQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20218_length_4413_cov_2.868059"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKH81_01295"
     CDS             complement(<1..448)
                     /locus_tag="PKH81_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96702.1"
                     /translation="MTSLLEKLRAAANRGAFLETKPFTLLLERCITVLEHEQALVRPS
                     DSTALSGGLIRLDKNIPTVVLPDLHGRFDFFLSVMESPLFGQESLAQAVQNHQAQILC
                     LGDGFHSELRGKERWISALDEYINGYKTHASMDQEMTESFVLMEMVM"
     gene            complement(445..969)
                     /locus_tag="PKH81_01300"
     CDS             complement(445..969)
                     /locus_tag="PKH81_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96703.1"
                     /translation="METFLGLSAVIQNHIKQIAKTSGMTEGDETLEVLAQAWEEKRKC
                     FEDGLSENQLEEVSFFSKDEEKGAVALTYSGSLITLGPLVDGYRKADYTSIGLRKDVP
                     STASEQMASLADDVEVDSVISFTKGPIQRSSPIYKIAVSMEDLQAEEEEALLTQITQD
                     IAEEFVEVNKTIIQ"
     gene            1034..1936
                     /locus_tag="PKH81_01305"
     CDS             1034..1936
                     /locus_tag="PKH81_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ96704.1"
                     /translation="MTEKKFTDLPARIKTHIESVLRETSETVNEEATERYARAWLDKY
                     ELFQSQIHAVGMEIVEKLAISDKRAAILLTYSGSLVSFGTAKPDTRALEYASIKFRAD
                     VPDFITAKGVTIKNEIKQDSIAEFTDCPLKQSSEIYRIAVCPEGTSETEQDLRVREAT
                     IFLTNGFIKINRTVTDIQERNVEQFTSKAIVSFISKKHGLTQVATKAILDDYLSTIES
                     GVLLGERVTVGKLGTLFLKARSARQARVMKNLVTGEDILVPAKVACLVPKFSLSKTLK
                     EKAALVDTAIIANGQSDEDEDDND"
     gene            1936..2943
                     /locus_tag="PKH81_01310"
     CDS             1936..2943
                     /locus_tag="PKH81_01310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNQ96705.1"
                     /translation="MIETKDNPFSPLTPDLILQAAEQGIGVSLESVVHPCNSYVNRVY
                     SLKAEEGDRYVIKFYRPGRWSLSAIEDEHNLLQECAKEEIPVVPPVVIKGLNVPSSSI
                     QTIGVIEGIFFSCFPFKGGRTFDITSDDDYLRIGRLIGNLHRISKNGNAQSRVTFSPQ
                     NVYTSFCEPFSRSSALYPEVREEFLELCQNVLFTSEAYFKADSFIRIHGDCHRGNILD
                     RGNEDLLLIDFDDMMIGPAVQDLWLLLPDHLASSRREMNLLIEGYETFCSFNRDSLRL
                     IETLRFMRNLYFLSWMDSQKEDAGFSQRYPDWGSKAFWITEMEDLRYQGGHALETLLQ
                     D"
     gene            complement(2940..3860)
                     /locus_tag="PKH81_01315"
     CDS             complement(2940..3860)
                     /locus_tag="PKH81_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNQ96706.1"
                     /translation="MIPVAIDDPVLQERFSTMHPDGMTVFLLGNGDMRGAFFHGTRFV
                     STMRAQHALGLLETMVLGQACLCGALLIPTMKGRDRSVFRYDTQGPAAGFSVEASSDG
                     KVRGYLLQNPIPLEKEPENWDLAPYFGEGTVTVIRYPEGYTEPVTGKIEIMHRNIALD
                     LAEYFLRSEQTATAFNSGIQFDREGRVSGAGALYLQTMPGAHPDIVAAAEHAFAAAPS
                     LGQWFAEGGDREDIIFGLFRELQPVIALERSITFDCPCNRETFLSRIRSLGEAELSDM
                     RSTGPDPLEVYCHYCSSVYHIPLKELDRPI"
     gene            3932..>4413
                     /locus_tag="PKH81_01320"
     CDS             3932..>4413
                     /locus_tag="PKH81_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ96707.1"
                     /translation="MTTATKSSVIRNRAALPMFALTLPIILENSFRILVSSVDTFMLG
                     SWSPKAVAAAGMMAQYIFLITILMNVICIGTSIVLSQYLGAKREEEATKVTQASAVMI
                     SLTALTISALVLFGTRPLLSQYDIEVEVRDMAWRYFTIFGGFGSLFMAFSLLQGTVLR
                     A"
BASE COUNT         1208 a         1057 c         1025 g         1123 t
ORIGIN      
        1 ccatgaccat ctccataaga acaaagcttt ccgtcatttc ctggtccatg gaggcatgag
       61 ttttgtaccc attaatatat tcatcaaggg cagaaatcca ccgctcctta ccacgtagct
      121 cggaatgaaa cccatcgcca aggcacagga tctgtgcttg atgattctga actgcttgtg
      181 cgagagactc ctgtccaaac aagggagatt ccataaccga aagaaaaaaa tcaaaccgtc
      241 catgaagatc aggaagaacc actgtgggta tgttcttgtc aagccgaatc aaaccgcccg
      301 aaagagcagt ggaatcagat ggccgaacaa gagcctgttc gtgttcaagg acggtaatgc
      361 atcgctcaag taaaagagta aatggttttg tttccaaaaa cgcgccgcgg tttgccgcgg
      421 cgcgtaactt ctccaaaaga ctggtcactg gataatcgtt ttattaactt ccacgaattc
      481 ctcggcaatg tcctgcgtaa tttgtgtcaa caacgcttct tcttcctctg cctgaagatc
      541 ttccatgctc accgctattt tgtaaatagg agaactccgt tgaatgggtc ctttggtaaa
      601 actgattacc gagtcgactt caacatcatc agcaagagaa gccatttgct ccgatgcagt
      661 agatggcacg tccttgcgta aaccaattga tgtgtagtcg gcctttctgt atccgtccac
      721 taaaggacca agggtaataa gagagcctga atacgtgagt gcaaccgctc ccttttcttc
      781 atctttagaa aaaaacgaaa cttcttcaag ttgattttcg ctcaatccat cctcgaagca
      841 ttttcttttt tcttcccatg cttgagcgag aacctctaag gtctcatctc cttctgtcat
      901 tccagatgtc ttcgcgatct gtttgatatg attttgaata acagccgata aaccgagaaa
      961 agtttccata aaaacctcct gaattgggct atactgtagc acgagattct ggaaagtcat
     1021 gaaggaggct ataatgactg aaaaaaaatt cactgacctg cccgcgcgaa ttaaaacgca
     1081 cattgaatct gtgttgaggg aaacctcgga aacagttaac gaagaagcaa cggagcgata
     1141 cgcacgagcc tggctcgata agtatgagtt gttccaaagc caaattcatg ccgtaggaat
     1201 ggaaatcgtg gagaaactgg cgatatcgga caaacgcgca gcaattctcc ttacgtattc
     1261 cggttcattg gtcagctttg gaaccgcgaa acctgatacg cgcgctcttg aatatgcgtc
     1321 cataaaattt cgtgctgatg ttccggattt tattaccgct aaaggcgtaa caattaaaaa
     1381 tgagataaag caagactcca ttgccgaatt tactgactgt cccttaaagc aaagctcgga
     1441 gatatatcgt attgcggttt gccctgaagg tacgagcgaa accgagcaag accttcgggt
     1501 tcgggaggca accatatttt tgaccaatgg ttttattaaa ataaacagaa ccgtaacgga
     1561 tatacaagaa aggaacgtag agcagtttac ctctaaggca attgtttctt ttatttccaa
     1621 aaaacatggt cttacccagg tggcgacaaa ggcaatcctt gatgattacc tttctactat
     1681 agagtctggt gttttactgg gagagcgggt taccgttgga aaattgggaa cgctcttttt
     1741 aaaggcccgc tctgctcgac aagcacgtgt gatgaaaaat cttgtaaccg gagaagacat
     1801 tcttgtcccg gcgaaagtcg cctgccttgt cccgaagttt tcgctttcaa aaacgctcaa
     1861 ggaaaaggca gcccttgttg acaccgcaat tattgcaaac ggacaatcag acgaagatga
     1921 ggatgataac gactaatgat tgaaacaaag gacaatccct tttccccgct tacgccggac
     1981 cttattctgc aagctgcaga acaaggcatc ggcgtatcat tagaaagcgt tgtacatcca
     2041 tgcaacagct acgtcaatcg tgtgtactct cttaaagcgg aagagggtga tcggtatgta
     2101 atcaaatttt accgaccggg acgctggagt ctttctgcga tagaagatga acacaacctt
     2161 ttgcaagaat gcgccaaaga ggagatacct gtcgttcctc ctgttgtaat taaagggcta
     2221 aacgtaccgt cgtcctcaat tcaaaccatc ggtgtaatag agggaatctt tttttcctgt
     2281 tttcccttta agggaggaag gacctttgat ataacctctg atgatgatta tttaaggatc
     2341 ggaaggctca ttggcaatct ccatcgcatt agcaaaaatg gcaatgcgca gtctcgcgtt
     2401 acctttagtc cacaaaacgt atacaccagt ttctgcgaac ccttttcccg ctcgtccgcg
     2461 ctctatccag aagtgcgcga agaatttctt gagctctgtc aaaacgtcct ctttacatca
     2521 gaggcgtact tcaaggcgga ctcatttatt cgcattcatg gagactgcca tcgggggaac
     2581 atcctggatc ggggaaacga ggatttactg ttaattgact ttgatgacat gatgatcgga
     2641 cctgcggttc aagacctgtg gcttcttttg ccggatcacc ttgcctcatc aaggagagaa
     2701 atgaatctgc ttatagaagg gtatgaaact ttttgttctt ttaatcgaga ttctctgagg
     2761 ctgattgaaa ccttgcggtt tatgaggaac ctgtatttcc tgtcctggat ggacagtcaa
     2821 aaagaagatg ccggtttttc ccagcggtat ccagattggg gaagcaaggc cttttggata
     2881 accgagatgg aagacctccg ctatcaggga ggccatgccc ttgaaacgct cctgcaggat
     2941 taaatcgggc gatcaagttc cttcaggggt atgtgatata cgcttgagca ataatggcaa
     3001 tacacttcaa gaggatcagg gcccgtagat cgcatatcag aaagttcagc ctcgcccagg
     3061 gaccgaatgc gcgaaagaaa ggtctcccga ttgcaaggac aatcaaaggt gatagagcgc
     3121 tcaagcgcaa tcaccggttg tagttccctg aataatccaa agatgatgtc ttccctatcc
     3181 ccgccctcgg caaaccattg cccaagagaa ggtgccgcgg caaatgcgtg ctcggcagcc
     3241 gctactatat cgggatgtgc gccgggcatg gtttgcaaat aaagggctcc cgcgccactt
     3301 acccgtccct cccgatcaaa ctgaatgcct gagttaaacg cggttgccgt ttgttccgag
     3361 cgcaaaaaat attcggcaag gtcaagggcg atgttgcgat gcataatctc tatcttgccg
     3421 gtaactggtt cggtgtaccc ttcggggtag cgaatgaccg ttaccgttcc ttccccaaaa
     3481 tacggggcaa gatcccagtt ttccggttct ttctccagag gaatggggtt ttgtaaaaga
     3541 tatccgcgaa cctttccatc gctggatgct tcaacgctaa aaccggcggc agggccttgc
     3601 gtatcatagc gaaagacaga gcgatcgcga cccttcatgg tcgggattaa cagggctcca
     3661 cacaggcatg cttgacccag gaccatcgtt tccaaaagtc caagcgcatg ttgcgccctc
     3721 atagtggaaa caaagcgagt tccatggaaa aaggctccac gcatatcgcc gtttcccaaa
     3781 agaaacaccg tcatgccgtc gggatgcatg gtagaaaaac gctcttgtaa aactggatcg
     3841 tcaatagcta caggtatcat aggtagccgt agtataccgg ttcaggggga gtggacgcaa
     3901 gcagggaaaa gggatacact cccgctaata tatgacaaca gcaacaaaat caagcgtcat
     3961 caggaaccgc gcagcgcttc ctatgttcgc ccttaccctg ccgataatcc tggagaattc
     4021 cttcaggatt ttggtttctt ctgtggacac ctttatgctt ggctcctggt ctcccaaggc
     4081 agtagccgct gcaggaatga tggcccagta tatcttttta atcaccatcc tcatgaacgt
     4141 catctgcatc ggaacgagta ttgtgctttc ccaatacctg ggggcaaaac gagaagaaga
     4201 agctacaaaa gtaacccagg cgagcgcagt aatgataagc ctgacagccc ttaccatatc
     4261 agccctggtt ctctttggta cccgacccct cctttcccaa tacgatatcg aagttgaagt
     4321 tcgggacatg gcctggagat acttcaccat ctttggtgga ttcggctctc tttttatggc
     4381 attcagcctc ttgcagggaa ctgtcttgcg tgc
//
LOCUS       DAOQXQ010000045         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20225_length_4412_cov_2.658940, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000045 DAOQXQ010000000
VERSION     DAOQXQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20225_length_4412_cov_2.658940"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKH81_01325"
     CDS             complement(<1..268)
                     /locus_tag="PKH81_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNQ96708.1"
                     /translation="MIISSIDLKDGKVVQLRQGKDLVLERDNPDELIREFDRYGEIAI
                     IDLDAALRNTRSDGTTANTAILKRLLRQGSVRVGGGIRDAKKAKE"
     gene            complement(286..903)
                     /locus_tag="PKH81_01330"
     CDS             complement(286..903)
                     /locus_tag="PKH81_01330"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNQ96709.1"
                     /translation="MERIACIERKTGETQINLTLNLDGTGICSVDCPIGFLSHMLSSF
                     CKHGLFDLSGSLQGDLEVDQHHLVEDTGISLGQAFDRALGTRAGIFRSGFCIYPMDET
                     LARAAVDFGGRPWLECSLTLSGAALVASGDPSAAFQTDTVDDFWQGFTAGAKCNLHLD
                     ILRGRSDHHKIEAAFKAASRAIRQAVSIDARTGGAIPSTKGTLTH"
     gene            complement(921..1775)
                     /gene="hisG"
                     /locus_tag="PKH81_01335"
     CDS             complement(921..1775)
                     /gene="hisG"
                     /locus_tag="PKH81_01335"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNQ96710.1"
                     /translation="MDKLKILLPKGRIYENVAAMFAEAGISIYLPDRTYRPIVNQDDL
                     EAKVMKPQNIGKLLELGAHDIGFTGIDWIRETGADVVEVMDLGFDKVRIVAAVPSEID
                     DATLRSKRMIVATEYVNNAEVWLKKEKLNYLIVRTYGATEVFPPDDADMIIDNTATGR
                     TLQENGLRIIDTIMESSTRMFASKEAMANPQKSKKIQELKMLFEAALAARNRVMLEMN
                     VSKARFSDLVSGIPAMKSPTVAPLYGDDGYAIKIVVKKSEVPLLMPKLKALGATDILE
                     YELRKVMP"
     gene            2022..3344
                     /gene="argA"
                     /locus_tag="PKH81_01340"
     CDS             2022..3344
                     /gene="argA"
                     /locus_tag="PKH81_01340"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNQ96711.1"
                     /translation="MMHTERIRDVIRYIRKFKGSTIVIQMDDCLVDASLFSGHIHDLA
                     LLYETGIRIILVPGAKNHISQVLDSYGVQWTMHDGVRVTSDEGMNFIKMAAFDISNRI
                     MTRLSGEKLTAVIGNWVRARGRGVIDGLDYGSAGLVEKIQIESLNRALDDGFIPIIPC
                     IGWTSTGKPYNISSTELAVATAIAVKAEKLFFVAKGATISINDYDLLPTTAASPEGRL
                     AAISVDDIPAFITANKDVVNPAFPTDTLIELLEQGRDACLGGVTRSHILDGNIDGVLP
                     AEVFSDLGSGTMIFKNNYGTFRTMTRDDIPAVLSLMNPFVEKKILLPRSSEELEQKAQ
                     DFMVYEIDGGIRACAALHVYHGNTGEIAALAVDEAYAHMGIGPRLMNLLIERGKNAGF
                     KAIFVLTTQTADWFERFGFKSAEIARLPAERKEKWRADRGSKVLFLDF"
     gene            3413..>4412
                     /locus_tag="PKH81_01345"
     CDS             3413..>4412
                     /locus_tag="PKH81_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNQ96712.1"
                     /translation="MSEADPAARIILEDGTEFEGVSRAWHHSSAGEVLVYTGETGLPA
                     LLTDPALKGAILVLTRPVAGVTGIPDEENDTLALGTTVESAEVQIAGLVCSSLSLESY
                     HYRSKKTLARWLKRHQIPVISGIDTSALVRRINLRGNLRAKILVSQAPDISFSAAKTG
                     EVALQSSVKRTIVYGTGPKRILVVDCGARNSVIRSLVTPRTSVIRVPAASDWSSLDCD
                     GIVVAGGPGDPTTCEKAISILSQKLSLSIPIFATGQGAVILGIAAGGAAYRMARGHHS
                     PSVPCVCLETGRCYLTAQNHGYGIRSDSLPSGWDPLFLNNADQSLEGFSTKKGLIKG"
BASE COUNT         1120 a         1189 c         1013 g         1090 t
ORIGIN      
        1 gttcctttgc tttttttgca tcccgaatgc caccgcccac gcgtacagat ccctggcgca
       61 acagcctctt aagtatcgca gtgttagcgg ttgttccgtc gcttcgggtg tttctgagcg
      121 cggcatcaag atcaataatg gcaatttcgc cataccgatc aaattctcgt atcagttcat
      181 caggattatc ccgctcgagc acaagatcct taccctggcg aagctgaaca acctttccgt
      241 cttttaaatc aattgaagag ataatcatcg tgtatttcct taaaactagt gtgtaagggt
      301 acccttggta ctcggaatag caccgcctgt gcgggcatcg atagataccg cctgccggat
      361 agcgcgagaa gctgctttaa acgcagcctc aatcttgtga tggtcgctgc gcccgcgtaa
      421 aatatccaga tgcaagttgc acttggctcc cgcagtaaat ccttgccaaa aatcatcgac
      481 agtatcagtt tgaaacgcgg cagaaggatc accggaagca acaagagctg ctccgctcaa
      541 ggtaagagag cattcaagcc agggccgtcc gccaaaatca accgcagcac gggcgagtgt
      601 ctcatccatg gggtagatac aaaatcccga gcggaaaatt cctgccctcg taccaagagc
      661 gcgatcaaag gcttgaccca aactgatacc ggtatcctcc acaagatggt gctgatctac
      721 ttccaaatct ccttgcaagg aaccggaaag atcaaagaga ccgtgcttgc aaaaagaaga
      781 gagcatatga gacaagaatc cgatgggaca atccaccgaa cagatacctg ttccatccag
      841 attcagcgtc aggttgatct gtgtctcccc ggttttccgc tcaatgcacg caatccgttc
      901 catcttgtct cctccgccgg ttatggcatt acctttcgca attcatattc caatatatca
      961 gtagccccca aggccttaag cttgggcatc aacaggggca cttcgctttt cttgacaaca
     1021 atcttaatag catatccatc atcaccatag aggggggcca ccgtcgggct cttcatggca
     1081 ggaataccac tgactaaatc agagaaccgg gccttggaaa cattcatctc gagcatcacc
     1141 cgattacgcg ctgcaagggc tgcttcaaaa agcatcttga gctcctgaat cttcttgctc
     1201 ttctggggat tcgccatagc ctctttagag gcaaacatcc tggtggagct ttccatgata
     1261 gtgtcgataa ttcgtaaacc attttcctga agagttctgc cagttgcggt gttgtcgata
     1321 atcatatccg catcgtccgg aggaaacacc tcggtggcgc cataggtacg gacgataaga
     1381 taattcagtt tttctttttt aagccatacc tcggcgttat tcacatactc ggtagcaaca
     1441 atcattcgct tggatctaag ggtcgcgtca tcaatttcgg agggaactgc agcaacgatg
     1501 cggaccttgt caaaacccag atccatcacc tcaaccacat ctgcccctgt ttctcgaatc
     1561 cagtcaattc ccgtaaatcc aatatcatgt gcccccagtt ccaggagctt gccgatattc
     1621 tgaggcttca ttacctttgc ctcaagatca tcctgattaa ctatggggcg ataggtgcgg
     1681 tccggcaaat aaatagaaat ccccgcctcg gcaaacatag ccgcgacgtt ttcataaata
     1741 cgtcccttcg gcagcaaaat cttgagcttg tccatacaat cctccactct cgttacaatt
     1801 ttttagaaaa aaaaagccgc tgttccctgg cgggcacagc ggctgctgtt acatgcttac
     1861 agcgtcaccg attacctcag cccggaatgg cacaggagga aacatgatga tggtgaaacg
     1921 ccttgaaaat attcacggga atagtaaaac atgaatggct aaatggtgtc aatacaggat
     1981 tccccgtttg acagacagga cggactgccg tacgataagc catgatgcat acagaacgga
     2041 ttcgcgatgt aatccgctac atacgaaaat tcaagggttc taccattgtt attcaaatgg
     2101 acgattgtct ggtagatgcc tctcttttct caggacatat tcacgatctg gcactcctgt
     2161 atgaaactgg aattcgcatt atcctggttc ccggcgctaa aaatcacatc tcgcaggtcc
     2221 tcgattccta cggagtacaa tggacaatgc acgacggtgt tcgagtaaca agcgacgaag
     2281 ggatgaactt tatcaaaatg gcggcctttg atatctctaa ccgtatcatg acacggctat
     2341 caggcgaaaa actcaccgct gtcattggta actgggttcg tgcgcgtggc cggggcgtta
     2401 tagacggtct tgattacgga agcgctggcc tggtcgagaa aattcaaatc gagtccctta
     2461 accgtgccct ggacgacggc tttattccca ttatcccctg tatcggttgg acttccaccg
     2521 gtaaacccta caatatctcc tcaacagagc ttgccgtggc caccgcaata gcggtaaagg
     2581 cagagaaact gttctttgtc gccaagggag cgactatctc gattaacgat tacgatctcc
     2641 ttcccaccac agccgcatct ccggaaggcc gtcttgccgc tatctcggta gacgatattc
     2701 ctgcctttat tacggcaaat aaagatgtgg taaatccggc ctttcccacc gacacgctta
     2761 ttgagctttt ggaacagggt cgcgacgcct gccttggagg ggtaacccgc tcgcacatcc
     2821 tggacggaaa catcgatggg gtcttacccg cggaggtttt ttccgatctg ggatcgggaa
     2881 ccatgatatt caaaaacaat tatggtacct tccgaaccat gaccagggac gacatccctg
     2941 cggtcctatc ccttatgaat ccctttgtag agaaaaagat ccttttaccc cgttcctccg
     3001 aggagcttga gcaaaaagcc caagatttta tggtatacga gattgatggc ggaatccgtg
     3061 cctgcgcagc cctccatgta tatcatggca ataccggaga aatagccgcc ctcgcggtgg
     3121 acgaagccta tgcccacatg ggaattggcc cacgtctcat gaatctgctc attgaacggg
     3181 gtaaaaatgc aggattcaag gcaatctttg tgttaactac acaaacagca gactggtttg
     3241 agcgttttgg attcaaatca gccgaaatcg ctcgtcttcc agctgaacgc aaggaaaaat
     3301 ggagggcaga tcgaggatcc aaggttcttt ttttggattt ctaaattttt tttcttgttt
     3361 tttacaaata ccggacctat cattcctttg tatataaaaa ggagtcatgc gtatgtccga
     3421 agccgatccc gctgcccgaa ttatccttga agatggtact gaatttgaag gcgtttcccg
     3481 agcctggcat cactcttctg ccggagaggt tcttgtgtat acgggagaaa ccgggcttcc
     3541 agccctcctc accgatccag ccctgaaagg tgcaatcctg gtgttaactc gtccagttgc
     3601 gggcgttacc ggcataccgg acgaagaaaa cgacaccctg gcccttggca ctaccgttga
     3661 atcagcagaa gttcaaatcg ccggtttggt atgctcatcc ctatccctgg aaagttatca
     3721 ctatcgttca aaaaagaccc ttgccagatg gcttaaacgc caccaaatcc ccgttatcag
     3781 cggtatagac acctcggccc tggttcgccg aattaacctc aggggcaatc tccgggcaaa
     3841 gattctggta tcccaggccc cggacatttc ttttagcgct gccaaaaccg gggaggttgc
     3901 gcttcaatca tcggtaaaac gcacaattgt atacggtacc ggacccaaac gcattctggt
     3961 cgtcgattgc ggggcgcgta actctgtcat cagaagcctg gttacccccc gaacctctgt
     4021 tatccgcgtc ccggccgcat cagactggag ctcccttgac tgtgacggca ttgtcgttgc
     4081 cgggggacct ggggatccaa ccacctgcga aaaggcaatc tccatccttt cacaaaaact
     4141 ctcactatcc atccctatat tcgcaacggg ccaaggggca gttatcctgg gaatcgcagc
     4201 tggtggagcg gcctatcgta tggctcgtgg gcatcatagc ccttctgtac cctgtgtctg
     4261 cctggaaaca ggccgctgtt atctaaccgc tcaaaatcat ggatatggaa tccgttcaga
     4321 ctcccttcct tccggatggg atccgctctt tcttaataac gcagatcaat ccctcgaggg
     4381 cttctccaca aaaaaaggcc taatcaaggg gg
//
LOCUS       DAOQXQ010000046         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20381_length_4386_cov_2.491803, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000046 DAOQXQ010000000
VERSION     DAOQXQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20381_length_4386_cov_2.491803"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..609)
                     /locus_tag="PKH81_01350"
     CDS             complement(7..609)
                     /locus_tag="PKH81_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96713.1"
                     /translation="MKRLIITMMLTLAGVCLLGAQEVESPKQPESRYEYEPIRSGDQY
                     IRLGLGVNFPLFFTGPGGVETDTKLDMGGAGFIGFSRFITSHLSLGGELGFAFNATLG
                     ENMLVYVPLTFKITYELVYDRLHFPLSLSPGLGLQSYKSRNYFGLLLRPEVACFFQYS
                     PEWSFGVSSAWDILPQWYDTSSDNRVGHFVNVVVGLRYHF"
     gene            725..1606
                     /gene="folD"
                     /locus_tag="PKH81_01355"
     CDS             725..1606
                     /gene="folD"
                     /locus_tag="PKH81_01355"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNQ96714.1"
                     /translation="MSAVIIDGYTVAATVRAQVAKSVSALVGQGITPCLSVVLVGEDP
                     ASVSYVSGKEKALREVGMKDASYRLPATTTEEELITLIHRLNADTTVHGILVQLPLPR
                     HINEARVIMAINPEKDVDGFHPVNIGNMVIERPCFLPCTPHGVMYLLKNAGIETRGAH
                     AVVVGRSNIVGKPLSILLARKDSNATVSVCHTGTRDLASITRQADILIAAAGKPRMIS
                     ADMIKPGAAVIDVGVNRVEDASSPKGYRLVGDVDFDAALEVASFITPVPRGVGPMTIA
                     MLMENTLLSAERVARSS"
     gene            1606..2392
                     /gene="folE2"
                     /locus_tag="PKH81_01360"
                     /pseudo
     CDS             1606..2392
                     /gene="folE2"
                     /locus_tag="PKH81_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
     gene            2389..3765
                     /gene="miaB"
                     /locus_tag="PKH81_01365"
     CDS             2389..3765
                     /gene="miaB"
                     /locus_tag="PKH81_01365"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNQ96715.1"
                     /translation="MTFFFETYGCQMNHAESSAVKNLLRSRQWQEASSADSADLVIIN
                     TCSVRITAETRVVGRIAHFTACKRERDLTILVMGCMAERLHEEFMTRFPAVDYVVGMF
                     ERSRFEAIFSAIESRYSKGDPLDYIPSPDAAGGYRFFSNSLEEGAFQAYIPIMNGCNN
                     FCSYCIVPYVRGREVSRDITSILSDLDNLAARGVREVTLLGQNVNSYRWQDSLSGAMI
                     DYPALLERIARHLDNTSGIKWVRFMSSHPKDLSDDVIAVIARERVFCRYIHLPVQSGS
                     NRILGEMNRRYTREHYLSLVDRIRTAIPEVTISTDILVGFPGETEEDQQQTLNLVETV
                     GYDAAFMYHYNPREGTKAFDMPNWISEPVKKARLDQVIRLQHSLAAQRMRSRVGSEPI
                     ALIESTSRNNPDELFGHTEFGDMVVFASPADHALIGRFVRVQLTLLRGRTFRAKIVEI
                     EPEVAERR"
     gene            3787..4044
                     /locus_tag="PKH81_01370"
     CDS             3787..4044
                     /locus_tag="PKH81_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96716.1"
                     /translation="MPLKFLVFMIVMVFVAFFIGFNLENRCDISIVFHTYHDVPVVLS
                     VLCAFILGVLVSGFSLLSSRKSPRKDQKPVKPSKKTPYGID"
     gene            4044..>4386
                     /locus_tag="PKH81_01375"
     CDS             4044..>4386
                     /locus_tag="PKH81_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96717.1"
                     /translation="MHRYAHPVWYLLCFFCLLPVFSAPSKTAQDQVSRALAAADATRV
                     QASLEGALARTTNPSDMQYLLSSLASFHERTGRPDLAALRFQEAAQAAPTDGSESLLL
                     DAARCALSGGDT"
BASE COUNT         1046 a         1065 c         1085 g         1190 t
ORIGIN      
        1 aaggccttaa aagtggtaac gaagtcccac gaccacgttg acaaaatgac cgacgcggtt
       61 atcagagcta gtatcgtacc attgaggcag gatatcccat gccgaagaaa cgccaaagga
      121 ccattcaggc gagtattgaa aaaagcatgc aacttctggc ctcaagagca agccgaagta
      181 attgcgcgat ttgtaggatt gcaagcccag tccgggagac agggaaaggg gaaaatggag
      241 tcggtcatag accagctcgt aggtaatttt aaacgtaagc ggaacgtaca caagcatgtt
      301 ttcgccaaga gtagcattaa aggcaaaacc aagctctccg cctaacgaaa ggtgagaggt
      361 aataaagcgc gaaaagccga taaaacctgc accgcccatg tcaagctttg tatcggtttc
      421 aacgccgccc ggtccggtaa aaaacaaggg aaaatttact cccaatccca gccgtatata
      481 ctggtccccc gatcgtatcg gttcgtactc gtatcgtgat tctggttgtt tcggtgattc
      541 aacttcctgg gctcccagga gacatactcc cgccaaggtc aacatcatgg tgatgatcaa
      601 acgtttcatt ggcacactat acctcatttt ttccgctatt gtctatcaaa gccggtacat
      661 ggtacaacaa tgtatccagt ccatacgtta cggcggacac tcccaataga tgtcgaggta
      721 tattatgagc gcagtaataa ttgacggcta tacagttgcg gctacagtcc gtgcccaggt
      781 ggccaagtca gtatccgccc tcgtcgggca aggaattact ccctgtcttt cggtggtttt
      841 ggtaggcgag gacccagctt ccgtttcgta tgtaagcggt aaagaaaaag ccctgcgcga
      901 ggtggggatg aaggacgcca gctatcgcct tcctgccaca acaacagaag aagaattgat
      961 aacacttatt catcggctta acgccgacac aactgttcac ggtattctcg ttcagcttcc
     1021 gcttccccgt cacatcaacg aggcccgcgt tattatggct atcaatccgg aaaaggatgt
     1081 agatggtttt catccggtaa atattggcaa tatggttata gagcggcctt gttttcttcc
     1141 atgtactcct catggcgtta tgtatttgtt aaagaatgcg gggatcgaaa ctcgaggtgc
     1201 ccatgcagtt gtcgttggaa gatcaaatat tgtcgggaag cccctttcca ttcttcttgc
     1261 gcgtaaggat agcaacgcaa cggtaagcgt ctgtcataca gggacgcggg accttgcttc
     1321 aattactcgc caagctgata ttcttattgc cgcggcgggt aaacctcgaa tgattagcgc
     1381 agacatgatt aaacccggcg cagctgttat tgacgttggg gttaaccggg tagaggatgc
     1441 ttcctctcca aagggatatc gtttggtagg cgatgttgat tttgacgcag cgctggaagt
     1501 agcgtctttt attactcctg taccccgtgg cgtcggtcct atgaccatcg ctatgcttat
     1561 ggaaaacacc cttctttctg ccgaacgggt tgcccggagt tcgtaatggt tgacattcaa
     1621 agcgagaggg acgaccgtga tattcccctg caaaaggtgg gtatcaaggg tttgcgctat
     1681 ccggttcagg tgctcgaccg ggaaaacaag gttcagcaca ccacagcctc ggttaatctc
     1741 tacgtgaacc ttccgcatca ttttaaggga acccacatga gccggtttat cgaggtgttt
     1801 catcaacacc gagcggcttt ccatgcccta tttcctggat atgctggaag aaatacgccg
     1861 ttctttggac gcggcccgtg cctttggcga aatgagtttt ccctttttta ttgataaaaa
     1921 agccccggtg tcggagcaac gcgccttaat gagttatgat tgctcctttg acggtctggt
     1981 gtcctcaacg ggagagcggt ccttccatgt atcagtgtct gttcctgttc aaaccgtatg
     2041 tccgtgttcc cgggccatca gcgagtatgg tgctcataat caacgtggta tcgtcagggt
     2101 aaaattggaa cttggtcctt ttttctggat agaggacctg gtctctctgg tagaaaactg
     2161 cgcctccagc gcagtgtatt cgctgcttaa acgagaagac gaaaaatacg tcaccgaaac
     2221 tgcctacgat aatcccaaat ttgtggaaga tctggttcgc gatgttatgt tggctatccc
     2281 gaccctgggc tcctttccct ggtacagcgt cgaggcggaa aacatagaaa gtattcataa
     2341 ccacagtgcc ttcgcctatg ctgaatcttc agatcaaggg gcaattcggt gacatttttc
     2401 tttgaaacct atgggtgcca gatgaatcat gccgaatcgt ccgcggttaa aaatctcttg
     2461 cggtcccggc agtggcaaga ggcatcaagt gcggattcag ccgatctggt tattattaat
     2521 acctgttcgg ttcgtataac cgccgaaacc cgggttgtgg gtcgaattgc ccattttacc
     2581 gcctgtaagc gagagcgaga tcttaccatt ttagttatgg gctgcatggc agagcggctt
     2641 catgaggaat ttatgacacg gtttccggct gtcgattatg tagtcggtat gttcgaacgt
     2701 tcccgtttcg aagctatttt ttctgctatt gaatcccgtt attccaaagg tgatcccttg
     2761 gactatattc cttccccgga tgccgccggt ggctatcgtt ttttttccaa ttcccttgaa
     2821 gaaggtgcgt ttcaggccta tatccccata atgaatggat gcaataattt ttgttcctat
     2881 tgcatagtcc cctatgtgcg tggtcgcgaa gtttcccgcg acatcacatc aatcttgtcc
     2941 gatcttgata acctggctgc ccgtggtgtt cgcgaagtta ccttattggg ccaaaatgtt
     3001 aactcctatc gctggcagga tagtctctcc ggggctatga tcgattatcc cgcccttctg
     3061 gaacgtatcg ctcgccatct tgataataca agcggtatta aatgggtccg atttatgtcg
     3121 agccatccca aagatctttc agatgatgta atagcagtaa tcgctcggga aagggtgttc
     3181 tgtcgctata ttcatctgcc ggtacagagt ggatcaaatc gtattctggg cgagatgaat
     3241 cgacgataca cccgggagca ctatctttcc ttggtcgatc gtatccgtac cgctataccg
     3301 gaagtcacca tttccaccga tatcctggta ggtttccccg gagagaccga agaagatcaa
     3361 cagcaaaccc taaatctggt agaaacagtc ggatatgatg cggcctttat gtatcactat
     3421 aatccgaggg agggcacaaa ggccttcgac atgcccaact ggatttccga accggttaaa
     3481 aaggctcggc tggatcaggt tattcgtctg cagcattcac ttgccgctca acgtatgcgc
     3541 agtcgcgtgg gaagtgaacc aattgccctt attgagtcaa cctcccgcaa taacccggat
     3601 gaattgttcg ggcataccga atttggggat atggtagtgt ttgcaagtcc tgccgatcat
     3661 gcccttattg gacggtttgt tcgagtccag ctcactctct tgcgcggccg aacgttccgt
     3721 gcgaaaatag tggaaattga accggaagtc gccgaaaggc gatagttctg ttcccggagg
     3781 agaccgatgc cccttaaatt ccttgtattt atgattgtga tggtgtttgt ggcgtttttt
     3841 atcgggttta acctggaaaa tcgctgtgat atatcgattg tctttcatac gtatcatgat
     3901 gttccggttg tgttaagtgt gttatgtgcc tttattcttg gggtgcttgt ctccggcttt
     3961 tcattactga gctcccgaaa atctccccga aaggatcaaa aaccggtaaa accatccaaa
     4021 aaaacgccat acgggatcga ctgatgcatc ggtatgctca ccctgtatgg tatctgttgt
     4081 gttttttttg tttgttgcct gttttttctg ccccgtccaa aactgcacag gaccaggttt
     4141 ctcgcgccct tgccgccgca gatgccactc gggtacaagc aagtttggaa ggcgctcttg
     4201 cgcgaacgac aaatccatca gatatgcagt accttctttc ttccctggct tcctttcacg
     4261 aacgcaccgg aaggcctgat cttgctgcac ttcgctttca ggaagctgca caggcagccc
     4321 caacagatgg atcggaatca ttgttgctgg atgcagcccg ttgtgctctc tcgggtggag
     4381 atactg
//
LOCUS       DAOQXQ010000047         4374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20466_length_4374_cov_3.226673, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000047 DAOQXQ010000000
VERSION     DAOQXQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20466_length_4374_cov_3.226673"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..497)
                     /locus_tag="PKH81_01380"
     CDS             complement(156..497)
                     /locus_tag="PKH81_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNQ96718.1"
                     /translation="MKLITAYIQGPKLNDVKQELFRNGIYKMSVMTAMGCGQQLGYQE
                     NYRGVVEEVNLLKKIRIEIAVNDEFVKPTVDAIIAGARTGNIGDGKIFVQALEECIRI
                     RTGETNTVAIG"
     gene            complement(508..1845)
                     /locus_tag="PKH81_01385"
     CDS             complement(508..1845)
                     /locus_tag="PKH81_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNQ96719.1"
                     /translation="MSEAGITLQEVKIAIDTLWVLLATFLVFFMNLGFAMVESGLCRS
                     KNTVNILTKNFIVFAIATISFWVIGFGLMFSDGNSFTGLSGLLFASGMDNSPATGDAY
                     TGVYSSLGWAGVPFWAKFIFQLVFCGTCATIVSGAVAERIKFHSFLIFSFIICAIIYP
                     LIGHWIWGGGWLMELGMFDFSGSTVIHSAGGWAALAGILMLGPRIGKYKMDGSTKPIY
                     GHNMTSATIGALILWFGWFGFNPGSTLSANPEALAHIAMTTCIAAAMAILSSTVTSWI
                     TQKKPDLSMIINGCLAGLVAITAPCAFVSMTSAAVIGAIAGTLVVFSVFFWDKLRIDD
                     PVGALSVHLVNGVFGSLAVGLFAEQSIGTDLGTGALVSNGLFIDGSVSQLLAQGAGVL
                     AVALFVFSVSMVVWAILKKTLGLRVTKEEELAGLDIGEHGMEAYSGFQIFNNL"
     gene            2028..2615
                     /locus_tag="PKH81_01390"
     CDS             2028..2615
                     /locus_tag="PKH81_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ96720.1"
                     /translation="MIITHKVWSAWFFIIAKAFVRGFSLLWISGTMWSMMIDEYRVIL
                     DRARARHAETLKQARHLSKLNKKNYDQKVWEIHEEVFASIDCTKCGNCCRALGPRFRH
                     TDVKIICKAIGEDPRSFTKEYLKDDEEGVGYVLKELPCPFLNEDNTCREYELRTLSCR
                     EYPHTLSRGIQKHLVRLAHDSLICPAASLILDRLL"
     gene            2661..4016
                     /locus_tag="PKH81_01395"
     CDS             2661..4016
                     /locus_tag="PKH81_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HNQ96721.1"
                     /translation="MDTLLTTLSNAFTSFFAFKAYVILPVIMLLLAFIARMRFKKALL
                     AAIRVGAGFAGVFIVFSFFVAQISPAIESMIRLRSLAYPVIDIGWPPLAAITWSSALT
                     PLFLVAIMLLNVLMLVTGVTRNLYLDLWNYWHFAFLGVMVQSLTGSVLCAILATLAIA
                     VFTIKTTEWSAPYVKREMGLDGIAVSPLSVSTFLPYSVALDRLFDHIPGLKKLNWNPS
                     VNRQKDSLVSLLSEPMIIGILVGVFLSFLAGYSLKQTLETAVNFAAVMFLLPQCGGLI
                     GSGMGEISHAFKVLVEKRFPHMENLSIAMDTGFLLTNPSVIATGLILMPLSILLAFIL
                     PGNRVIPLGDLPNLISVISLTVLIMRGNVIRAVLAGIPIVATFMIFASRLAPVFTAEA
                     ARSGVDMGTGSSEITAFTDGGNQLRFWVYYLFDGNWIAIAAIPVVLALMIHAWRRSRA
                     L"
     gene            complement(3979..>4374)
                     /locus_tag="PKH81_01400"
     CDS             complement(3979..>4374)
                     /locus_tag="PKH81_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ96722.1"
                     /translation="TILGDIPRIKAAGEQINQVLVNILANALHAIQSEKGAHGEIKIL
                     TGRTFDTVWCEIENSGPPIPAEHIDRIFDLFFTTKEDQFGTGLGLNLCKDIIENHHSG
                     SLTLRSANPVTFRIELPIKGPESDAMHES"
BASE COUNT         1071 a         1026 c         1051 g         1226 t
ORIGIN      
        1 cgctcggctt tggcatcatt ggcttgtggg gatgcctgct tgcagacgag acaattcgcg
       61 ctgcgataaa tctgtggaga tggaaatcag ggcgctggct tggcaagacg gttattgaaa
      121 aagaaaccgc cccggcatga cggggcggta tgtaattagc caatcgctac ggtatttgtt
      181 tctcccgttc gtattcgtat acattcctca agggcttgta cgaagatttt gccgtctcca
      241 atattgcctg ttctagctcc ggcaatgata gcgtctaccg ttggtttaac aaactcgtcg
      301 tttaccgcaa tctcgatgcg aatcttctta agaagattaa cctcttccac aacaccgcga
      361 taattttcct ggtacccgag ttgttgcccg cagcccatgg cggtcataac cgacatcttg
      421 taaattccgt tacggaataa ttcctgcttt acatcattga gcttggggcc ctgaatatat
      481 gctgttatca atttcataat ccgctcctta gaggttgtta aagatctgga atccagagta
      541 ggcttccatt ccatgctctc caatgtcgag tcctgccagt tcttcttctt tagttactcg
      601 tagacccaat gttttcttaa gaattgccca cactaccatg gatacagaga agacaaatag
      661 tgcgaccgca agtactcctg ctccctgggc aagaagctgc gagacgctac cgtctatgaa
      721 caatccgttt gaaacaagag caccggttcc caggtccgtg ccaattgatt gttcggcgaa
      781 aagaccgact gccagtgatc cgaatacacc gtttaccaag tgtaccgaca gggctccaac
      841 tggatcgtct attctaagct tgtcccagaa gaagaccgag aatacaacca gcgtgcctgc
      901 aatcgctcca ataacagccg cgcttgtcat ggacacaaag gcgcagggtg cggttatggc
      961 aacaaggcct gcaagacatc cgtttattat catggaaaga tccggtttct tttgggtaat
     1021 ccatgaggta accgtggaac tcaagatagc catggcagcg gcgatgcagg tagtcatcgc
     1081 aatgtgtgca agggcttcgg gattggcaga cagggttgat ccggggttaa aaccaaacca
     1141 tccgaaccac aggattaaag cacctatggt ggcactggtc atgttgtggc catagattgg
     1201 tttggtactg ccatccattt tgtactttcc gatgcgtggg ccaagcataa gaataccggc
     1261 aagggctgcc caaccgcctg cggaatgaat taccgtggag ccggaaaaat caaacatacc
     1321 caattccata agccagccgc cgccccatat ccagtggccg atgagcggat agataatggc
     1381 gcaaatgata aacgaaaaga taaggaatga atggaatttt atccgttcgg ccacagcgcc
     1441 tgagacaatc gttgcacagg taccacagaa cacaagctga aagataaact tggcccaaaa
     1501 gggaactcct gcccatccaa gggagctgta tacgccggtg tatgcatctc cagtcgccgg
     1561 ggagttatcc ataccggagg cgaacaataa tccgcttaat ccggtaaagg agtttccatc
     1621 agaaaacatt agaccaaaac caatgaccca gaagctgatc gtcgcaatag cgaatacgat
     1681 aaagtttttg gtaaggatgt ttaccgtgtt cttggaacgg caaagccctg actcaaccat
     1741 tgcaaagccc aggttcataa agaacaccaa aaaagtcgca agtaagaccc aaagggtgtc
     1801 tatcgcgatt tttacttcct gcagtgtaat gcctgcttca ctcatgtagt cctcctgtct
     1861 tgaactatcc cttatagtaa gcaagcgcca tgccaactgt ataaaaaagg tgcgggacac
     1921 tcacttgtcg gcgggtatgg agatcagaac atcggtagtg ccggattttt gctcgtaaga
     1981 tgtcagtatg cggtagaaca cagcaatgaa gcatagtgta caagtgaatg ataattacgc
     2041 ataaagtatg gagcgcatgg tttttcatca tcgcaaaggc ttttgtaagg ggcttttccc
     2101 ttctttggat ttctggcact atgtggtcga tgatgatcga tgaataccgc gtgatcctgg
     2161 atcgcgcacg cgcacgccat gcggaaacac tcaaacaggc acgacacctt tccaaattaa
     2221 ataaaaaaaa ctacgaccaa aaggtgtggg agattcacga agaggtattt gcctctatcg
     2281 attgtaccaa atgtggcaat tgctgcaggg ccctgggtcc gcgattcagg cataccgatg
     2341 ttaaaattat atgtaaggcc attggtgaag atccccggtc ctttacaaaa gaatatctta
     2401 aagatgatga ggaaggtgtt gggtatgtgc ttaaggagct tccctgtccc tttttgaatg
     2461 aggacaacac atgcagggaa tatgagctgc gaaccttatc ctgtcgcgaa tacccgcata
     2521 cgctttcccg cggaattcaa aagcatcttg tccggcttgc tcacgacagc cttatttgcc
     2581 cggcagcttc cctgatttta gatcgattgc tgtagcacgc tcttctggac agcgtccttt
     2641 tcatagccta cactgatgga atggacacat tactcacaac tctttcaaac gcctttactt
     2701 ctttttttgc ctttaaggcc tatgttattc ttccggttat catgttgctt ctggccttta
     2761 ttgccaggat gagattcaaa aaagccctgc ttgcagccat ccgtgttggc gcgggctttg
     2821 ccggcgtttt tattgtattt tctttttttg tcgcgcaaat tagtcccgcg attgagtcaa
     2881 tgattcgtct gcgatccctt gcctatcccg ttatcgacat tggctggccg cctcttgcgg
     2941 ctatcacctg gtcaagtgcg ttaacaccgc tgtttcttgt tgcgattatg ttgttgaatg
     3001 tgctaatgct ggtaaccggc gtaaccagga atttatatct ggatctatgg aactactggc
     3061 actttgcctt tcttggtgta atggtacaat cccttaccgg tagtgtcttg tgtgcaattc
     3121 ttgcaacatt ggctattgcg gtgtttacta taaaaactac cgaatggtca gccccgtatg
     3181 tcaagcggga gatgggactc gatggtattg ccgtaagccc tctgtctgta tctacctttt
     3241 tgccctattc agttgcgctg gatcgtcttt ttgatcatat acccggactt aagaagctta
     3301 actggaatcc gtcggttaat cggcaaaagg actcgctggt atcactgtta agtgaaccga
     3361 tgatcatagg aatccttgtt ggtgttttcc tttcgttcct tgccggttat tctcttaagc
     3421 aaaccctgga gacggcggtg aattttgctg cggttatgtt tttgttgcca cagtgcggag
     3481 ggttaatcgg ctcgggaatg ggagagatat ctcatgcctt taaggtcttg gtggaaaagc
     3541 gttttccgca tatggaaaac ctttctattg caatggatac cggcttcttg ttgactaatc
     3601 cctccgttat cgcgactgga cttattttaa tgccgctttc cattttgctc gcctttattc
     3661 ttcccggtaa cagagtgatt ccccttggtg atttaccaaa cttgatttcg gtaatctctc
     3721 ttaccgtgct tattatgcga gggaatgtaa tacgagcggt gttggcgggt attccgatag
     3781 tagcaacctt tatgattttt gcctcacgtc tggctccggt atttaccgca gaagccgctc
     3841 gctctggcgt ggacatggga acgggttcgt ccgagattac cgcgtttacc gatggtggga
     3901 atcagcttcg attttgggtg tattatttat tcgatgggaa ctggatagcg attgctgcca
     3961 ttccggtagt gcttgccctt atgattcatg catggcgtcg ctctcgggcc ctttgatggg
     4021 cagttcaatc cggaaggtga ccggattcgc cgatctaagg gtaagagacc cgctgtgatg
     4081 attttcaata atgtccttac agaggttaag tccaaggcct gttccaaact gatcttcctt
     4141 ggtggtaaaa aacaggtcaa agattcgatc gatgtgttca gccggaattg gaggccctga
     4201 gttttcaatc tcgcaccata cggtatcaaa agttcttcct gtaaggattt taatttcgcc
     4261 atgcgcccct ttctccgatt gaatggcatg gagggcattg gccaaaatat tgactaagac
     4321 ctggttgatt tgttctcctg cagccttgat tcgggggata tccccaagga ttgt
//
LOCUS       DAOQXQ010000048         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20542_length_4360_cov_3.051568, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000048 DAOQXQ010000000
VERSION     DAOQXQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20542_length_4360_cov_3.051568"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1276)
                     /locus_tag="PKH81_01405"
     CDS             complement(56..1276)
                     /locus_tag="PKH81_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HNQ96723.1"
                     /translation="MTKSERRILETLATKYSRSAHAQGGAPLRLSPEKSFADLLCAGP
                     DIYESFLEAAEKLQADGIISLTWKRYRTGEEISSIILQSPEGLFSRLERPLPSKIAQS
                     AQHTAREAMENAPDEISKSFFIWLAANIRAQDVSTGERELDLCAIIKDFAHLTNALTI
                     HAKDGFLCGITPRALSITLFSDSKRIECLLSQIAPLLSRARRALVPVPDTSAVERNFP
                     ETLIAGTLRLHLQDDKTVSNSSGSVIGIPLVTATRIRRIECPTHTLSHPPRLLSIENL
                     ETFYAIATRSGPPLAEAYLYTGGHPNRPVQKLLASFSHSGFSFIHAGDLDPEGILILQ
                     EIMGIAKKSVTPLKMDKATFLSHLAHSRELEDTALTRVAMIKDETRSLDGVEELLTQI
                     LETGRGVEQEIITY"
     gene            complement(1273..>4141)
                     /locus_tag="PKH81_01410"
     CDS             complement(1273..>4141)
                     /locus_tag="PKH81_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNQ96724.1"
                     /translation="FWIGKSIDISSVPVLTDAKRPLSWRQFRDLLDSRQVASYESKRE
                     YLRNLTDLLGVFRRMSEYNPYLESFTRSVSFTPLVSVDRFVCDYILEERTLDISIMKT
                     NLESYKEAERQANATVRKIDCLTEISSLEKEYRRLQTNLAKQDYLKLYIDTIIARDKL
                     KQTEKDKQNVCIQIEQIMSQIEENTQRRNIQETSLREVQAALARDDAHTLYLSLKEKI
                     ESLQKEKEQAKEDSHRFNTIIDQCKSLLEGTGISGKKKSTDNSHTSNREILEHYSDDL
                     EAARAESEENRYTTERTLDETEQALRECVKELNDLERGIRKYPESSASLASALKENGI
                     DAWILSDLAEITDPQWANAIEGWLNTLRFAVVVEPSFFQQALEVYDSLPKTVGGVALP
                     NIARLKTKADEARKGSLASLVSSDNPWARIYLTAILGEVMTADLDSLKNHSKAITKDC
                     MSYSNHTATRIKEEVWRNHWLGKRAREQRLTQLKTERSSLESKKKDLIILRDNLIEIL
                     RAYSQAIRSLADVRPLSEADSRIITLEKRIEEAKNDLSHIDISSSLALEEKRIALTRV
                     ITELSAERDKLTTDQERARSRNEYLVKEKEQQEIKVQESLHDFESFCADKIAELSWLE
                     EYANDRLKRSSPEDIELDYAPARKRLETRSNTTLAQYRKKVSEYTNQFSCYASVEIED
                     YTALAETLDHLERSELPNYRDRIIGAREDAEKEFREHFIAKLNEYIIEAKESFREINE
                     TLKDLAFGNDHYSFSLEERSDRRGQIRIVQKAAEVTSYEGGLFEQIIDPQERKATEEL
                     FEKIIRSDLNSAEMRSICDYRTYFSYDIRIRDAKAIDPATGKPVELSLSRVLREKSGG
                     ESQTPYYVAIAASFYRFFKDKSDSTIRFVLFDEAFDKLDDERIRRIIEFYSSMGIQLM
                     MAVPPGKIEAIAPLMDHVSIVSRIGQQARIREYRSFAGDAE"
     assembly_gap    4142..4151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4152..>4360)
                     /locus_tag="PKH81_01415"
     CDS             complement(<4152..>4360)
                     /locus_tag="PKH81_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96725.1"
                     /translation="KARFNLAAGDRRGGRDLIGYVRCKIGSESTEYLRGDTVAHIMLS
                     FTDGKSDFTAAVCVEAFSDGRISER"
BASE COUNT          986 a          972 c         1072 g         1320 t
ORIGIN      
        1 gctgcgcggc tattcttggt tttcagaccc cgctgggatg agtactcctt aatttttaat
       61 acgttattat ctcctgctct acaccccggc cggtttccaa gatttgtgtc agcaattctt
      121 cgaccccgtc aagcgaacgg gtttcgtcct ttatcatggc tacacgcgta agcgccgtat
      181 cctcgagttc ccgggaatgg gcaagatgag ataaaaaggt cgctttgtcc atcttgagcg
      241 gtgtgactga tttttttgcg atgcccatta tttcttgcaa gatcaagatg ccttccggat
      301 caaggtctcc ggcgtgtata aaggaaaacc ctgaatggct aaacgaggcc agaagttttt
      361 ggactggccg gttagggtgt cccccggtat acagatacgc ttctgccagg ggcggaccgg
      421 atcgagttgc gatggcataa aacgtttcaa ggttttctat agaaagaaga cggggtggat
      481 gggacaaagt atgggtaggg cattcgattc gtcttatcct tgttgcggta acaagaggta
      541 tgccgataac gcttccggat gagttgctca ctgttttgtc gtcctgaaga tgcagacgta
      601 gcgttcctgc tataagagtt tcggggaagt tacgctcaac tgcagaggta tcgggtaccg
      661 ggacaagggc gcggcgtgcg cgagaaagta gcggggcaat ttgtgaaagc aggcattcaa
      721 ttcgctttga gtcggaaaaa agcgttatag acaaagccct tggcgtaata ccgcacagaa
      781 atccatcttt ggcgtgtatg gtgagcgcat tggtaagatg cgcaaaatcc ttgattattg
      841 cacagaggtc aagctcccgc tcgccggtag atacatcttg cgcccggata ttggcagcga
      901 gccagatgaa aaaagatttc gagatttcat caggtgcgtt ttccattgcc tccctggcag
      961 tgtgttgtgc gctctgtgct atcttcgatg ggagggggcg ctcaagacgg gaaaaaagtc
     1021 cttcgggact ttgaagaata attgatgaga tttcttcacc cgtccgatac cgtttccagg
     1081 ttaaagaaat aatcccgtct gcctgaagtt tctccgcggc ttcaagaaat gattcatata
     1141 tatccggtcc ggcgcaaaga aggtcggcaa atgatttttc gggactcagg cgaagcggcg
     1201 cgcctccctg ggcatgggca gagcgtgagt acttggtggc aagggtttca aggatacggc
     1261 gttcgctctt ggtcattcag catctccggc aaaggatcga tattcacgga tacgcgcttg
     1321 ttggccaatg cggctgacga tgctgacatg gtccataagc ggggcgatag cctctatttt
     1381 acctggtgga acggccatca tgagctggat gcccattgat gagtaaaact caataattct
     1441 gcgtattctc tcgtcatcca gcttgtcaaa ggcttcgtca aaaaggacaa accgtatcgt
     1501 tgagtcgctc ttgtccttaa agaagcgata gaagcttgcc gcgatggcga cataatacgg
     1561 ggtttgggat tcccctcccg acttttcgcg aagcacccgg gacagagaga gttccacggg
     1621 ttttccggtt gcaggatcaa tcgcctttgc gtcgcggatt ctaatgtcgt acgagaaata
     1681 ggtacgataa tcgcatatac ttcgcatttc agctgagtta aggtcggagc ggattatttt
     1741 ctcaaagagt tcttcggtgg ctttcctttc ttggggatcg attatctgtt caaaaaggcc
     1801 tccttcatag ctggtaacct cggctgcttt ttgcacaatc cttatttgtc ctcgtctgtc
     1861 tgaacgttcc tccagagaaa aactgtagtg gtcgtttcca aaggcaagat ccttaagtgt
     1921 ttcgttaatc tcgcgaaagc tttcttttgc ttcgatgata tactcgttca gcttggcgat
     1981 aaaatgttct cggaattcct tttccgcatc ctctcttgcc ccaatgatac gatcgcgata
     2041 gttgggcaat tctgatcgtt caaggtgatc aagggtttcg gctagagctg tgtaatcctc
     2101 aatttctacg gaggcatagc aagaaaactg gttggtatat tcgctgacct tttttcgata
     2161 ttgggcaagg gttgtattgc ttcgtgtttc taatctcttc cgtgcaggag cgtaatccag
     2221 ttcaatatct tccgggcttg atcgtttaag tcgatcatta gcgtattcct ctaaccagga
     2281 aagttcagct atcttgtcag cacaaaagct ttcaaaatca tgcaggcttt cctgcacttt
     2341 tatttcttgt tgttcttttt cttttaccag atattcattt cttgagcgcg ctcgttcttg
     2401 atctgtggta agtttgtctc gttccgcgga aagctctgtg attacacgag ttaatgcgat
     2461 ccttttttcc tcaagcgcaa gtgacgagga gatatcgatg tgcgacagat cattttttgc
     2521 ttcctcgatt cgtttttcca atgtaattat tcgactgtcc gcttctgaaa gaggccggac
     2581 gtccgctaat gagcgaatcg cctgagaata ggcgcggaga atctcgataa ggttatctcg
     2641 taatataatg agatctttct tcttgctttc caaggaggac cgctctgtct taagttgggt
     2701 aaggcgttgt tctctcgctc gtttacccag ccagtgattc ctccaaactt cttccttgat
     2761 tcgcgtcgca gtatggttcg agtagctcat gcaatctttt gtaattgctt ttgaatgatt
     2821 tttgagtgag tcaaggtctg cggtcattac ctccccaaga attgcagtaa ggtaaattct
     2881 tgcccaggga ttgtcgcttg aaacaagaga tgcaagggaa ccttttctag cttcatcggc
     2941 ttttgtttta agacgggcga tgttgggaag ggcgactcct ccaactgttt taggtaatga
     3001 atcgtaaact tcaagggctt gttgaaaaaa tgagggctct acaacaacgg cgaatctaag
     3061 cgtattaagc catccttcaa ttgcatttgc ccattgcgga tcggttatct cggcaagatc
     3121 ggagagtatc caggcatcta taccattttc ttttagggcg ctggcaaggc ttgccgatga
     3181 ctcgggatat tttcgtatgc ctcgctctaa atcattgagc tcctttacgc attcacggag
     3241 ggcttgttct gtttcatcaa gggttctttc ggttgtatat cggttttctt cggattccgc
     3301 acgagctgct tccaggtcgt ctgagtagtg ttccaggatt tctctattgc tcgtgtggct
     3361 gttgtctgta gacttttttt tacctgatat tcccgtccct tccaggagag atttgcattg
     3421 atcgattata gtattgaagc gatgggagtc ttcctttgcc tgttcttttt ccttttgtaa
     3481 actttctatt ttttctttga gtgaaagata gagcgtgtgc gcgtcatccc gggctaaggc
     3541 tgcctgtact tcgcgaagcg atgtttcctg gatgttacgt ctttgagtgt tctcttctat
     3601 ctgcgacatg atttgctcta tttgtatgca tacattttgc ttgtcctttt ctgtttgctt
     3661 tagcttgtcc ctggcaataa ttgtgtctat atacaatttt aggtaatctt gtttagcaag
     3721 gtttgtctgg agtctgcgat actctttttc taatgatgat atctcagtaa ggcagtcgat
     3781 tttccggact gtcgcatttg cctgccgctc cgcttcctta taagattcca gattggtttt
     3841 catgatgctg atatcaagtg ttctttcttc caggatatag tcgcagacaa aacgatcaac
     3901 agatacaagc ggggtaaaac tgaccgaacg cgtaaaggac tcaaggtagg gattgtattc
     3961 gctcatgcgc ctgaatacgc caagaagatc ggtaagattg cggagatact cgcgttttga
     4021 ttcataagaa gcgacttgcc gagagtcaag aagatcgcgg aattgcctcc acgaaagcgg
     4081 tctcttggca tcagtgagca caggcacgct agagatatcg attgacttgc cgatccaaaa
     4141 gnnnnnnnnn nagcgttcag agattcggcc gtcgctaaaa gcttcaacgc agaccgctgc
     4201 cgtaaaatcg gattttccgt cagtaaaaga aagcatgatg tgggctaccg tatctcccct
     4261 aagatactca gtgctttcgc ttcctatctt gcatcgaaca taaccgataa gatcccttcc
     4321 tccccgacgg tctccggcgg caaggttaaa tctggccttt
//
LOCUS       DAOQXQ010000049         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_20867_length_4306_cov_2.829922, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000049 DAOQXQ010000000
VERSION     DAOQXQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20867_length_4306_cov_2.829922"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..3640)
                     /gene="rpoB"
                     /locus_tag="PKH81_01420"
                     /pseudo
     CDS             complement(138..3640)
                     /gene="rpoB"
                     /locus_tag="PKH81_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667607.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            complement(3802..4188)
                     /gene="rplL"
                     /locus_tag="PKH81_01425"
     CDS             complement(3802..4188)
                     /gene="rplL"
                     /locus_tag="PKH81_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNQ96726.1"
                     /translation="MAALTNDQILDAIAGMTILEVSELVKAMEEKFGVTAAAPVAVMA
                     GGAAAGAAPAEEQTEFTVTLKGLSADDKKIAVIKEVRTVTGLGLKEAKDLVEGAPKTL
                     KENVSKDEANKIKESITAAGGVVEIA"
BASE COUNT         1069 a         1083 c         1052 g         1102 t
ORIGIN      
        1 ggtttcaggc gatgcaagct ttatattaag actgtcaaaa tcctgcaatt ctctcatctt
       61 gtttcctccc ggaatatcga accaagagcc gatattccga tatttagaat acaaccaggg
      121 ctaatcgttg ttgcagatca gaagtttgat ccggcctttt cgatcaactc ggcatcgcgt
      181 tcagtcagcg gaatctgctt tccttcgcgt caaaaatggt gaaatcaagc gcgagaccgc
      241 gaagttcctg cacgagaacg ttaaacgact cgggaacgcc ggccgcggtg gacggctctc
      301 ccttgacgat ggattcatat atcttggaac gaccgttcat gtcgtccgac ttgatagtaa
      361 gaagttcttg cagtgtgtta gcggcgccat acgcttcaag agcccatact tccatttctc
      421 caaggcgctg accaccaaat tgggccttac caccaagagg ctgctgggta accagcgagt
      481 agggtcccgt ggagcgagca tgcatcttgt cgtctaccag gtgatgaagc ttcatgaagt
      541 agataacacc gacaaataca gggttatcaa acggaatgcc cgttcggcca tctcgaagca
      601 taaccttgga gtttgtgggg aaccctgctt ccgtaagttt tgcctcgatc tgttcgttcg
      661 agggagactg gaagataggg gactcatacc actcattgag cctaaggccg gctagaccaa
      721 gctcagactc catgatctgt ccgatattca tacggcttgg aacaccaagc gggttaagac
      781 agatatcaag cggcgtgcca tcctcaaggt agggcatatc ttcttcggga agaatgcgcg
      841 caacgatacc cttgtttccg tggcgtccgg ccatcttgtc accttcgcga agctttcgct
      901 ttgtcgcgat aagaaccttg actacctcgt caacgccggg attaagatcg tcgccttcag
      961 ttctcttaag acgctgaacg tcgataatct ttccctcaac tccgtgcgga acgcgcaaag
     1021 aagagtctcg cacttccttg gctttttcac cgaagataga gttaagaagc ttaaactcgg
     1081 gtgttgtctc agtttcactc ttgggggtta ccttaccgat aagaatatca cccgagcgaa
     1141 ccttggcacc tatgcggata ataccttccg catcgaggtt atcaaggctc ttttctgcga
     1201 tattcggaat atcacgagta agtttttccg gaccgagctt ggtttcccga acttcggtaa
     1261 cgaattcctt gatatggatg gaagtaaaca tatcttcctt aacgacacgt tgagatatca
     1321 aaatggcgtc ttcgtagtta tacccgttcc agggaacgaa tccgacaagg atgtttcgtc
     1381 ccagggcaag ctcaccatga taggtagcag gtccgtcggc aagaggagtt cccttttcta
     1441 ctttttgacc gggctgaacg accggacgtt gatgataaca ggtatcctgg ttggtcctct
     1501 gatacttaag aaggggatat tcgtctcgaa tctccttatc ttttacgccg gtcggtttaa
     1561 tgatgaccat ttctgcagag acaaactcga cagtacccgg gcgcttggcc ttaaccagaa
     1621 caccggagtc aaaggcgcat tttccttcca tacccgtacc aacacgaggc ggctccggga
     1681 atacgagcgg aacagcttgc cgctgcatgt ttgaacccat gagggcgcgg ttcgcgtcgt
     1741 cgtgttccag gaagggaata agcgaagcag acacagaaat aatctgcttg ggtgaaacgt
     1801 ccatatactg aacgtctttt ggaggacgag tggtgtaatc accgtgacga cggcaagata
     1861 cctgatcgac attaaagttt cctttattgt cgatagtagt ttgcgcctgg gcgatgtaat
     1921 acttgtcttc gtccatagcg gaaagataat caatctcgtt tgtggcaacg ccatttacta
     1981 tcttaacgta tggagcttcc aggaagccgt agtcattaac gcgagtatag ttcgccaacg
     2041 aaacgataag accgatgttt ggaccttccg gagtttcgat cgggcacatg cgtccataat
     2101 gggtgtaatg aacgtcgcgg acctcgaagc ccgcgcgatc gcgggaaaga ccaccaggac
     2161 cgagcgcgtt aagacgacgc ttatgagtaa gctctgccag gggatttacc tgatccataa
     2221 actgggaaag ttggcttgaa ccaaagaatt cttttatggc ggcgacaatc ggcttgatgg
     2281 agataagatc ctggggctta atggtctcgg tttccttaag actcatacgc tccttggcga
     2341 tacgttccat gcgcgaaaag gcggttttaa gggtattggt catcagctcg cccacagagc
     2401 gaacacgacg attgccaagg tggtcgatat catcgatgga ttcatcccca acatacacct
     2461 gaataaggta cttcatcgta gagatgatat cttctttaac gagggtgtag tctgcaaccg
     2521 gctccggata gtcaaacttc ttgttgagct tatagcgtcc cacccgtcca agatcatagc
     2581 gacgggagga gaagaacatt gaatgaaggt ctttttccgc agcttcgatg gtaatgggtt
     2641 caccgggcat aagaacggaa tacactgcgg caagcgcatc ttccttactc ggctcgtctc
     2701 cttctacatc cttaccggag tagcggattt cctcgcgctc aaagcaattg ataatcatgg
     2761 ctgaatcgag agagtcatcg gcaccaaaat caattacggt aatcttggtc accttattca
     2821 tgacaagctc gtcgatattg tgcggatgaa ttttttctcc agccttgtaa agcttctttt
     2881 cgttgccttc ctcgtccttg ataaacacgg cagaagcgag gaccttacca accagcttgt
     2941 cttgttttgc cctgtctgaa gaaacagtca tattggttgt tttatagaag caacgaataa
     3001 tgtcttcccg ggtctcgtag ccaagagccc taaggaagag cgtgcctaga atccgttttt
     3061 tacggtcgat cttggcatat accagctctt ttttctggtc aatctcgaac tcaagccagc
     3121 ttccgcgata gggaatgatg cggcttgaat acacaccttt ttcgtgacta aagataacac
     3181 cgggagaacg gtgtatctga gaaacaacaa cgcgttcggc gccattgatg ataaaggttc
     3241 cacgatcggt catgagcgga atatcgccca tgtatatgtc tttctgccgg atttctccgg
     3301 tctgctggaa catcaaattg atacgggcct taagaggcac cgcgtaggtt aaacccttct
     3361 gcttgcattc aagttcggaa aatttgatat tttcttcatc gagctcataa aactcgtact
     3421 cgaggagcat gtcaccgttc gggctttcaa tgggaaaggt ggaacgaaaa acgtcttcaa
     3481 gacccacctc ttccgaggac tcactagact tggtcttgtc tctctgaaga aatttctcat
     3541 atgacgaaag ctgaatatca atgaggtcgg gcaaatccat aaagctgcga atggttttgc
     3601 cgatatactg acggttgatc gcctgatttc ttgcgaacat cccatacccc ctaaaaaacg
     3661 aataaacacg atagcgaaga agcgggcatt acccgccact ccgttgacta caaaaggtcc
     3721 ccgccgaata actcggcagg aaccttaggg aaaaattgtt tctatgacgc atcctgccgc
     3781 aaagcaggat taaggacgaa atcaggcaat ctcgaccaca ccaccggcgg cagtgatcga
     3841 ttccttgatc ttgttcgctt cgtccttgga aacgttctcc ttgagagtct tgggcgcacc
     3901 ctcgacaagg tccttcgctt ccttgagtcc gagaccggta acggtgcgga cttctttgat
     3961 aacggcaatc tttttgtcgt ctgcagaaag acccttaagg gtaacggtaa actcagtctg
     4021 ctcttcggca ggagcggctc cagcggcggc gccaccggcc ataacggcaa cgggagcagc
     4081 ggcggttacg ccaaacttct cttccatagc ctttacgagt tcagagacct caagaatggt
     4141 cattcccgcg atagcatcca gaatctggtc attggttaaa gctgccatat tatcttctcc
     4201 ttacggtttc ttcctcgact ccgtacgggg tcgatgcccg ttgccgggct cttttagcgc
     4261 tgcaggacga cagctgccgg aagcggcgat ctcgccgccc ccgcga
//
LOCUS       DAOQXQ010000050         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_21279_length_4244_cov_2.776796, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000050 DAOQXQ010000000
VERSION     DAOQXQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21279_length_4244_cov_2.776796"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="dgcA"
                     /locus_tag="PKH81_01430"
     CDS             complement(<1..826)
                     /gene="dgcA"
                     /locus_tag="PKH81_01430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HNQ96727.1"
                     /translation="MAKAKKNSAGDASLEKQIYDLKQLLEISKSLNSVIDFSTLIEAI
                     LYTCMGQMKTLGVAIFTKKNFDASCFQLNRNYYGFELTNDHEYFIPEDHPFIKKLNTV
                     NACITVEDLAKMELEEDKPISALIALNPSLIVPLKAKNHITGLLVLGEQITSEPYSDY
                     EKEHIINIAALAAIAINNAGLLEMTTTDMMTHLKLKHYFYTVLMEKLELSEQQKIPLS
                     VLMLDIDFFKRFNDTYGHACGDVVLQMVASVVQQNTRNQDMAARYGGEEFCVMLCDT"
     gene            complement(833..2470)
                     /locus_tag="PKH81_01435"
     CDS             complement(833..2470)
                     /locus_tag="PKH81_01435"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HNQ96728.1"
                     /translation="MTFLQRLLVAIQDFLESIFSSSSPEYKKKHQLRILASQVRSTDP
                     PLFRDDGYLLPSFPSALLQIFQFLLPIREVLESTTASPDKRVSERYRDYLLELAMSQE
                     QRATRKNLTLSERAKALIAQPLSPERVIEDQAKQFSAFLKGLDNQTMRQAAHLLEKLD
                     ALADFCAFDFNSFFAFFDPAFQAHVGQSTTVDTPSFNPVEVVQIVPQLLDLYYLLSKV
                     DLSQSLCDVLSLLESKKTNQALSDDIRNRTARIFQALIWLLQKKISPGTVLAIIRLVK
                     EDPDYKPEQPQFQTDYLAMYKERLTDIFHSDSRKVLQEKQNSEIEQMVKNTFGDRELE
                     QVKGYNETTNTLLGEFSVFSLEWIKPLQIIKTFARHYFEPHFKQVLRSVIVEGYFNNR
                     TLQSSLSSAFYYCDAVCAKFNDFEALFDEKQPCSLKILTGYITEMEKGMDFEKPLQKM
                     VENMNGHAKTFIQEAVTQYSEVFNFSVIMMEDNKKPVPDYITNIRTLVASTKNIDSYR
                     YLEQESGVFRNFLEIMKKYAIVGTLSVPASLSEKTES"
     gene            complement(2467..3522)
                     /gene="dusB"
                     /locus_tag="PKH81_01440"
     CDS             complement(2467..3522)
                     /gene="dusB"
                     /locus_tag="PKH81_01440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNQ96729.1"
                     /translation="MDKPPLYHPVRIGPLALSGNIFLAPVAGYSDRAFRSLCIEYGAS
                     FTYTEMVSSEAITRDCTKTSLLLRRAAEETCYAVQLFGSDPSRMADATERVIDQVHPE
                     CIDINAGCPVPKVVKTGAGSSLTRDPQRLHAIVSAMVNRAGSIPITVKIRSGWDDSKL
                     SWEEAARAAIEAGAQAITLHPRTRAQGYEGRANWDLLSNLASLVHSNYPGIPVFGSGD
                     LFKPEDAQRMLDHTGCDAVMFARGATGNPFIFHQTRLLLTTGAYEDIPAKERMSAGWK
                     ELGILVEDLGEERACREMRKRFCAYSRGIEGGAELRSSLVLASSCAEYQSILTAHSLF
                     QGMTDLANSTPSVYNEP"
     gene            complement(3522..>4244)
                     /locus_tag="PKH81_01445"
     CDS             complement(3522..>4244)
                     /locus_tag="PKH81_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ96730.1"
                     /translation="IEQLRVIQEAKQASVTRIISICNSLHDFSQVYPTLKTASSVYHA
                     VGVSPSEVTNPGKDWMHIIEDALKQPNVVALGEIGLDYFRKFGDKRSQIELFITQLEM
                     ANKADMPVIIHNRDAGKDVLDILAERLPAAGGVLHCYSEDAEYARIALDLNVYFSFAG
                     NLTYRNARNLHETVLTLPLDRILVESESPFMVPAEFRGKRNMPSYTPSTVRFMAEMLE
                     MDIEVLAEQLWKNSCTLFRLPE"
BASE COUNT         1126 a          873 c         1017 g         1228 t
ORIGIN      
        1 ctgtatcgca gagcatgacg cagaactcct ccccaccgta gcgagccgcc atatcctgat
       61 ttctggtgtt ttgttgtacc accgacgcaa ccatttgtag tacaacatct ccgcaagcat
      121 gtccgtaagt atcgttaaag cgtttgaaaa agtcgatgtc gagcattagg accgacaggg
      181 gaattttttg ttgctcgctt aattcgagtt tttccattaa gactgtatag aagtagtgtt
      241 ttagcttgag atgagtcatc atgtcggtgg ttgtcatctc gagcaaaccg gcattgttga
      301 tagcgatggc agcaagggcc gcaatattga tgatgtgttc tttttcataa tccgaatatg
      361 gctcggaagt aatttgctct cccaatacca ataatccggt aatatggttc tttgccttga
      421 ggggaactat aagcgacgga ttaagagcga taagggccga tatcggtttg tcctcttcca
      481 gctccatttt tgcaagatct tctacagtaa tacaggcgtt gacggtattt agctttttta
      541 taaagggatg atcttccggg ataaagtatt catgatcatt ggtgagttcg aatccgtagt
      601 aattacggtt aagctgaaaa caggaagcat caaaattttt cttggtaaag atggccacgc
      661 cgagggtttt catttgaccc atgcaggtat agagaatcgc ttcgataagg gtggaaaaat
      721 ctataaccga attgaggctt ttagatattt ctaaaagctg tttaagatca tagatctgtt
      781 tttctagcga cgcatcccct gcactgttct tcttcgcttt ggccattatg tatcaactct
      841 ccgttttctc tgacaggctg gcaggtaccg ataatgttcc tactatagca tactttttca
      901 ttatttcaag aaaattacga aaaactccac tttcttgttc cagataacgg tatgagtcta
      961 tgtttttggt tgatgcgaca agggttctga tgttggtgat gtagtccggc accggttttt
     1021 tattgtcttc catcatgatc acgctaaagt taaaaacttc tgagtactgt gtaactgctt
     1081 cttgaataaa ggtcttggca tgaccattca tgttttctac cattttttgt aagggctttt
     1141 caaaatccat gcctttttcc atttcggtga tatacccggt aagaatttta agagaacagg
     1201 gttgtttctc atcaaagagg gcttcaaagt cattgaattt tgcacacacc gcatcacagt
     1261 aataaaacgc actagagagg ctactctgta aagtccggtt gttaaagtat ccctctacaa
     1321 tgactgacct aagaacttgc ttaaagtgtg gttcaaaata atgtctggca aaggttttaa
     1381 taatttgaag cggtttgatc cattccaggg agaaaactga aaactctcca agaagtgtgt
     1441 tcgtcgtttc attatagcct ttgacttgct caagttccct gtccccaaag gtgtttttaa
     1501 ccatttgctc aatttctgaa ttttgttttt cttgaagtac tttacgggag tcgctgtgaa
     1561 aaatatcggt gagtcgctct ttatacattg ctaaataatc agtttgaaat tgtggttgct
     1621 caggtttata gtccgggtct tcttttacca aacgaataat ggccagtact gttccaggag
     1681 aaattttctt ttgtagaagc cagataagtg cctgaaaaat acgggcggtt cgattacgaa
     1741 tatcgtcgga aagggcttga tttgttttct tggattccag gagagaaagt acatcgcaga
     1801 gggattgtga gagatctacc tttgacaata aatagtagag gtcaaggagc tgtgggacaa
     1861 tttgaactac ttctactggg ttaaaactgg gggtgtccac tgtggtagat tgaccaacat
     1921 gagcctgaaa ggcgggatca aagaaagcaa agaaagaatt aaaatcaaaa gcgcaaaaat
     1981 cagcgagtgc atcgagtttc tcgagtaaat gagcggcttg gcgcatagtt tgattgtcta
     2041 gtccttttaa gaaagcggaa aattgttttg cttgatcttc gattactcgc tccggggaaa
     2101 gtggttgcgc gataagtgct tttgctcgct cggacaaggt aagattcttc ctggtagctc
     2161 tttgttcctg gctcattgcc aattcaagca ggtagtctcg gtatctctct gagacccgtt
     2221 tgtctgggct tgcagtggtt gactcaagaa cttctctgat tggcaggaga aattgaaata
     2281 tttgaaggag ggctgatgga aaagagggca aaagataccc gtcgtcacgg aaaagtgggg
     2341 gatccgttga ccttacttgc gacgcaagta ttctgagttg atgttttttt ttgtactcag
     2401 gcgatgacga agaaaaaatc gattcaagga agtcttgtat agctacaagc aacctttgta
     2461 aaaacgtcat ggctcattgt agacagatgg cgttgaattt gcaagatcag tcattccctg
     2521 gaaaagggag tgtgcagtta atatagactg atactccgca caacttgagg cgagaacgag
     2581 gctgctgcgt agttctgcac caccttcgat tcctcgggaa taggcacaaa accgctttct
     2641 catctccctg caagcccgtt cttcgccaag gtcttccaca agaataccca actctttcca
     2701 tccagcggac atacgctctt ttgccggtat gtcttcataa gcgccagttg taagcagtag
     2761 acgtgtttga tgaaaaataa aggggttccc cgtggcaccg cgggcaaaca taactgcgtc
     2821 gcagccagta tggtcgagca tgcgttgagc atcctcgggc ttgaatagat ctcctgagcc
     2881 aaagacagga atgccggggt agttagagtg aaccagcgaa gcaagattgg ataaaagatc
     2941 ccagtttgct cttccttcat agccttgagc cctggttctg gggtgtaggg taatagcctg
     3001 tgctcccgcc tcgatagccg ctcttgctgc ttcctcccaa gaaagcttgg agtcgtccca
     3061 gccagagcgt attttaacag taatagggat tgatcctgct cggtttacca ttgctgaaac
     3121 aatcgcgtgc aatcgttgtg gatctcgggt aagggatgac cctgctccag ttttgactac
     3181 cttggggacg gggcagcctg cgttaatgtc gatacattca ggatgaacct gatcgataac
     3241 cctttcagtg gcgtctgcca tccgggaagg atctgacccg aacagttgaa cagcatagca
     3301 ggtttcctca gcggctcttc tgagcaagag gcttgtttta gtgcagtctc tggtgatcgc
     3361 ctctgaagat accatctcgg tatacgtaaa cgaagcgccg tattcaatgc agagggaacg
     3421 aaatgcacga tccgagtaac cggcaaccgg ggctaaaaaa atgtttccag atagggcaag
     3481 gggcccaatc cgcaccggat ggtacagagg gggcttgtcc atcattcagg aagccggaag
     3541 agtgtacagc tgtttttcca tagctgttca gcaagtacct ctatgtccat ttcaagcatt
     3601 tctgccataa aacgtacagt agaaggggta taagaaggca tattgcgttt ccctctgaat
     3661 tcagcgggaa ccataaaggg gctttctgat tctaccagaa tacgatccag ggggagtgtt
     3721 aaaacagttt catgtaaatt tctggcgttc cggtaggtaa gatttccggc aaaagagaag
     3781 tagacattaa gatccagggc tatacgtgca tattctgcgt cttctgagta gcaatgaagt
     3841 acgcctccgg ccgcaggaag tcgttccgca aggatgtcca gaacatcttt gccggcatct
     3901 cggttatgaa taattaccgg catgtccgcc ttatttgcca tttctaattg ggtaataaaa
     3961 agctcaattt gtgaccgttt atcgccgaat ttacgaaagt agtctaatcc aatttcgcct
     4021 aaagcgacaa cattgggctg cttaagagca tcttcaatga tatgcatcca gtccttgcca
     4081 ggattggtaa cttcggatgg ggatacacct acggcgtggt agacagatga agccgtctta
     4141 agggtaggat atacttggct gaaatcatga aggctattgc atatacttat aatgcgagtt
     4201 actgaggctt gtttggcttc ctgaataaca cgaagctgct caat
//
LOCUS       DAOQXQ010000051         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_21340_length_4236_cov_3.181057, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000051 DAOQXQ010000000
VERSION     DAOQXQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21340_length_4236_cov_3.181057"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKH81_01450"
     CDS             complement(<1..932)
                     /locus_tag="PKH81_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ96731.1"
                     /translation="MTLFAIAMVTGCKKNDGKTLYLYNWTYYTPDSVIEKFQEKYGVK
                     VVYDDYASNEDMYAKLKAGGSGYDIVIPSGDYVSIMKSQGMLEKIDLAQIPNVRYIKP
                     DTLTKATYDRTMEYSVPYYMGAAGIAVNREKVSEFEESWSIFARKDLAGRMSMMDDMR
                     EVLGDALAWLGYSVNTTDEAQLEEAFRVVNDLWKPNLVKFDAEGFAKSFASGEFWVVQ
                     GYAESVYAEVPAEMHDKIAFFIPKAEGGPMYIDSMCIPKGARNKELAHQFINFILEPA
                     IYAEFLDTFGFPATIHSEAGALQTVTPYYGADDLV"
     gene            complement(987..1868)
                     /locus_tag="PKH81_01455"
     CDS             complement(987..1868)
                     /locus_tag="PKH81_01455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ96732.1"
                     /translation="MKSRITPYQQFISRIAKKKPTSEPARHARRAFQNMNHLSLSNTV
                     MTLSVIFLFLPLAVIVFYSFNKAKGMVWTGFSLQWYSRLFMNSTKLWDSFGNSLIIAF
                     ASAFVATVLGTLASIGVNWYRFRGRGYIQTISFLPMVLPEVIIGISMLIFFSAVNVPL
                     GMLTIFIAHTTFCLPFVFLMVLARLDEFDFSIIEASRDLGATERQTLFKVIIPAIMPG
                     IVSAFLISVTMSLEDFVITFFVSGPGSTTLPLYVFSMVRYGISPVINALSFIMILGTM
                     LIAFFLRNFLKSIAASK"
     gene            complement(1865..2755)
                     /locus_tag="PKH81_01460"
     CDS             complement(1865..2755)
                     /locus_tag="PKH81_01460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ96733.1"
                     /translation="MSRPKMLASRNYGPAYSYPMGVWFSLFFVLPLAIIVTYSFMKKG
                     LHGGVEWQATLDAYRQMINSSYGKLFVRTLGVTFLSTALTIVISLPCGYAMARSRNQT
                     LLLFLIIIPFWTNSLIRINAWIAILGNEGFINETLKKIGIIQESLPLLYNQQSVILVL
                     VYMYLPYAILPIFTAMDKFDFSLLEAARDLGATKSQSMFKVLLPNVRAGIVTAIIFTF
                     IPIFGAYTVPLLVGGKDSYMIGNVIVDQVTKIRNWPLASAFSLIITVISTAGVLWMMT
                     TSVREAAKTKSTLGKQESGV"
     gene            complement(2752..3879)
                     /locus_tag="PKH81_01465"
     CDS             complement(2752..3879)
                     /locus_tag="PKH81_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ96734.1"
                     /translation="MKGCDVSIEGVSKSFGTFRALDDANIKINKGEFFSLLGPSGCGK
                     TTLLRIIAGFESPDTGIVSFDGRNIIGIEANKRESNTVFQNYALFPHLSVFENIAFPL
                     RIRKVPETEIRTKVMEYLRLVQLDAHVHKKPNQLSGGQKQRVAIARALINEPSVLLLD
                     EPLSALDAKLRQNLLVELDTIHDQIGITFIYVTHDQSEALSVSDRIAVMNQGKVLQIG
                     TPFEIYESPATEFVAKFIGETNLFDSRVVECVKHREDEYMVTLDIPELGEHIKVTDYD
                     ETLHGQQVSFTVRPEKIRITLEEPKTSGKEINVFKGIVEEPIYSGFQSKFYVKLDNGT
                     MMKVFKQHQNYLEDGPEITWKDTVYVSWSANDGYIVEVINQ"
     gene            complement(3958..>4236)
                     /locus_tag="PKH81_01470"
     CDS             complement(3958..>4236)
                     /locus_tag="PKH81_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96735.1"
                     /translation="ARDPFAGGVLTNPPAEIHQLFYEAPVPRRRDEWALRAIWERQEV
                     LTIAIAPMDEMSMTRSAILAEAGRPNSLPSSELSVISRAATLLRERKT"
BASE COUNT         1151 a          986 c         1015 g         1084 t
ORIGIN      
        1 accaagtcat ccgcgccgta atagggagtc actgtttgga gtgctcctgc ctcgctatga
       61 atggtggcgg ggaatccaaa ggtatcaagg aactcggcgt aaattgccgg ctcaaggata
      121 aagttaataa actgatgggc aagctctttg tttcgcgcac ccttgggaat acacatgctg
      181 tcgatgtaca ttggaccgcc ttccgccttg ggaataaaga aggcaatctt gtcgtgcatc
      241 tcggcaggaa cttcagcgta tacagattcg gcatatcctt gtacaaccca aaactcgccg
      301 gaagcaaatg actttgcaaa gccttcggcg tcaaacttga ccagattggg tttccataaa
      361 tcatttacca cgcggaacgc ttcttcaagc tgtgcttcgt cggttgtgtt aacagagtag
      421 ccaagccagg ccagggcgtc gcccagaacc tcgcgcatat cgtccatcat ggacattcgg
      481 ccggcaagat cctttcgtgc aaagatggac cagctttctt caaattcaga aaccttttcc
      541 cggtttaccg cgattccggc cgcacccatg tagtatggca ctgagtattc catggtcctg
      601 tcataggttg ccttggtaag ggtgtcgggc tttatatagc gaacgttcgg aatctgcgcc
      661 aaatcgattt tttccaacat cccctggctt ttcataatag atacataatc gccagaggga
      721 atgacgatat cgtaaccgga gccgcctgcc ttgagtttgg catacatatc ctcgttagaa
      781 gcatagtcgt cgtagacaac cttgactcca tacttctcct gaaacttttc tataacagaa
      841 tccggggtat agtacgtcca gttatacaaa tacagggttt tcccgtcatt ctttttgcat
      901 ccggttacca tagcgattgc gaatagcgtc atcgccagtg taagtactcc ggtgattcta
      961 cacactttac tcatagatta ctctccttat tttgaagctg ctatgctctt taggaaattc
     1021 ctgaggaaga atgcaataag catggttccc aatatcataa taaaagataa tgcatttata
     1081 accggcgaga ttccataacg taccatggaa aaaacataca acggcagggt tgtggaaccg
     1141 ggaccggaga caaaaaaggt aataacaaag tcttcaaggg acatggttac ggagatcagg
     1201 aaggcagaga cgatgccggg cataatcgcg ggtatgataa ccttaaagag ggtttgccgt
     1261 tcagtagcgc ccagatcccg ggatgcttcg ataatggaaa aatcaaactc atcaagccgt
     1321 gccaatacca tcaaaaaaac aaagggtaaa cagaaggtgg tatgagcgat aaatatggtc
     1381 aacatgccca gagggacatt aaccgcggaa aagaaaataa gcatggaaat accgataata
     1441 acctcgggaa gaaccatcgg caaaaaactg attgtctgga tgtatccccg accgcggaac
     1501 cgataccaat taacaccgat agaagcgagg gttccaagca ctgtcgcgac aaaggcagag
     1561 gcaaaggcga taatcaagct gttcccaaag gaatcccaca gctttgtgga gttcataaaa
     1621 agtctggaat accattgcag ggaaaaaccg gtccacacca tacccttagc cttattgaat
     1681 gaatagaaaa caataacggc cagcggtaaa aacaaaaaaa tcacactcaa ggtcattacc
     1741 gtattagaaa gggacagatg gttcatgttt tgaaaggcac gtcgtgcatg acgcgccggt
     1801 tccgaggttg gctttttctt ggcgatcctg gaaataaact gctggtaggg cgttatgcgc
     1861 gatttcatac tccgctctcc tgcttgccaa gggtgctctt tgttttagcg gcttcgcgaa
     1921 cgcttgtcgt catcatccat aatacacccg cggtacttat cacggtaata atcaaagaga
     1981 aggccgaagc aagtggccag ttacgaatct tggttacctg atccacaatc acgtttccga
     2041 tcatgtagga atcttttccg ccaactaaaa ggggtacggt atacgcgcca aaaattggta
     2101 tgaaggtaaa gattatcgcg gttacaatgc ccgcccgcac atttggtaac aggaccttaa
     2161 acatggactg gcttttagtg gcacctaaat ccctcgctgc ttccagaagg gagaagtcaa
     2221 acttatccat cgcggtaaaa atgggaagaa tggcataggg taggtacata tataccaata
     2281 ccagaattac cgactgttga ttgtaaagca gcggaagact ttcctgtatt atgccgattt
     2341 ttttaagggt ttcgtttata aagccctcat tccccagaat tgcaatccat gcgtttatcc
     2401 tgataagaga gtttgtccaa aacgggatga tgatcaaaaa caggagcaag gtttgatttc
     2461 ggcttctggc catggcatat ccgcagggaa gggaaataac gatggtgagg gctgtggata
     2521 aaaaagtaac cccgagcgtt cgcacaaaga gtttgccata gcttgaattg atcatctggc
     2581 gataggcatc cagcgttgcc tgccattcta cgccaccgtg aagccctttt ttcataaagc
     2641 tgtaggtcac gataatcgcg agaggaagga caaaaaaaag gctgaaccat acgcccattg
     2701 gatagctgta tgccggtccg taatttcggc ttgccagcat ttttggccgg ctcattgatt
     2761 tattacctcg acgatgtaac cgtcgtttgc gctccatgac acatagaccg tgtccttcca
     2821 ggtaatttcc gggccgtctt ccagataatt ttgatgctgc ttgaacacct tcatcatcgt
     2881 gccgttatcg agcttgacgt aaaattttga ttgaaatccc gaataaatgg gttcttcgac
     2941 aataccctta aagacattga tctctttacc gcttgttttg ggttcttcca gggttatgcg
     3001 aattttttcc ggcctgacgg taaaacttac ctgttggcca tgcagagttt catcgtagtc
     3061 ggttaccttg atgtgctctc ccagctcggg gatatccagg gtaaccatgt attcatcttc
     3121 tcgatgtttt acgcattcaa caacacggga gtcaaaaaga ttggtttctc caataaactt
     3181 ggcgacgaat tccgtagcgg ggctttcgta aatttcaaaa ggagttccaa tttgtaatac
     3241 cttcccctga ttcataacgg cgatgcggtc ggataccgac agcgcttcgc tttgatcgtg
     3301 ggttacgtag ataaaggtaa taccaatttg gtcatgaatg gtatcgagct caaccagaag
     3361 gttttgtcta agctttgcgt ccagggccga caggggctca tcaagaagga ggacgcttgg
     3421 ctcgtttatc agggccctgg cgatggcaac tcgttgcttt tggccgccag agagttgatt
     3481 cggtttttta tggacatggg cgtcgagctg aacaagacgc aaatattcca ttaccttggt
     3541 gcgaatctct gtctcgggaa cctttctgat tctgaggggg aaggcgatgt tttcaaatac
     3601 agataaatga ggaaagaggg cataattttg aaagacggta ttggactccc gtttattcgc
     3661 ctcgatccct atgatatttc gcccgtcaaa agagacaatt cctgtatcgg gagactcgaa
     3721 tcccgcgatg atacggagca gggtggtttt gccgcaccca gacggcccga ggagggaaaa
     3781 aaactctccc ttgttaatct tgatgtttgc gtcatcaaga gcgcgaaagg taccaaatga
     3841 tttggacacc ccttcgatcg aaacatcaca ccctttcaat ctgtttctct accctcccgg
     3901 aaggctatta ttgttatgta gtaacaaaat cgaatgtctt gcatttacgc tattctgtca
     3961 agtcttgcgc tctctaagga gagttgcggc acgggaaatg acggaaagct cggatgaagg
     4021 caggctgttt ggccttcccg cttccgcaag aatggctgaa cgagtcatgg acatctcgtc
     4081 cataggcgca atcgctatcg tcaaaacctc ttgccgctcc catatcgccc ttagcgccca
     4141 ttcgtccctt cggcgaggaa ccggggcctc gtaaaagagc tggtgaatct cggcgggagg
     4201 attagtcagt acgcctccgg caaaggggtc gcgagc
//
LOCUS       DAOQXQ010000052         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_21443_length_4220_cov_2.957503, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000052 DAOQXQ010000000
VERSION     DAOQXQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21443_length_4220_cov_2.957503"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2395
                     /locus_tag="PKH81_01475"
     CDS             <1..2395
                     /locus_tag="PKH81_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNQ96736.1"
                     /translation="ALTWKQFRDFHRTYYHPVNCKVFLSGNIPTERQLALLQEEFLCH
                     FTSAPAPALVSPSSDTDWPKEFVVPAPSGDGQDLSLVTAQLNWLLPAVTDVVTYMEAN
                     LLTEILLGHDGSPLNRVLLESALGEDVAPSSGIETEVLHLCFSVGLRGMAKDDVPAFE
                     QLVLDTLGDIAAHGVSSEELESAVRSIDFSNREVRRSGGPFSLTLMRRSLRGWIHGVG
                     PYETLRVIPAFEEVKTRLANKSDYIQSLVRRFLLDNPRRARVVVYPDSQWIARMDERV
                     DARVKLFESTLSGEEEQAFLSRQEDLFARQREADSEEQLALIPHLSRSDLPPVADSIP
                     SRITTCGKVPIVLHEQPVNKIIYVDLAVPIDVIKTSDYPLLPFFSAVLTSMAVDGLSW
                     VEASLLTARYTASLGAMLFSSQMVSGYQVPQSLEFVKGGRDFLMIRMKALEELSSEAI
                     SLMFRFFERADFSDTKRLLDLLNEYRNDLESSLAPAGNQYAVSRAAAFASRTKAVDEI
                     WNGLTQLRFIRELCAQAKDTKALVSLAERLTSIAQQLRSSGIVVNATAEATSMASLQA
                     QLSPHLEGYDGPAPYTPDQKRASELYCCIEPEFSGSFRLLISSPLQVGFAAAVLPSVP
                     YTHPDHPVEIVLGHWLSSGPLWERIRTTGGAYGAFSYPDSLEDLFVMATYRDPQPLAS
                     LAVFKEALEEASLHPIESKALDRIVTGAYSREIQPRSPSDKGFTAFIRLLYGITDEAR
                     KTKIAGILAVTPAELSACATRLLDSWPSVCQAVFAGEKMLKDGENDRFAGKVAKMTI"
     gene            2429..2632
                     /locus_tag="PKH81_01480"
     CDS             2429..2632
                     /locus_tag="PKH81_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96737.1"
                     /translation="MKKIDKEGYHILKQLVSDVQGAPYPNVIDNELYRIWYEHAQVVA
                     IQCLEYIDKNFPKDKDSEKLPSF"
     gene            2691..>4220
                     /locus_tag="PKH81_01485"
     CDS             2691..>4220
                     /locus_tag="PKH81_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNQ96738.1"
                     /translation="MNLDTCTIKTREAIGEASSIAQRRDHAQIETDHLLFALLSQEGG
                     IVPPLVERIGISASALLKELSDRLDRLPRVSGEAAQVHFSSQASKVLAKAEQEASSLK
                     DEYVSTEHLLLSIAASEDQAGKFLQEKGITRSALLSALKEVRGNQRITTEDPEATFQS
                     LEKYCRDLTALARQEKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LARRIVSGDVPDSLKGKKLLSLDLGALVAGAKFRGEFEERLKAVIHEVQKAEGSIILF
                     IDELHTLVGAGASEGSMDASNLLKPALARGELRAIGATTLNEYRKYIEKDAALERRFQ
                     QVYCPEPSIEDTISILRGLQEKYEVHHGVRIRDDALVAAATLSDRYITSRFLPDKAID
                     LVDEAASRLKMEIESQPTELDKVERKILQYNIEKVSLSKERDTASVERLAKLEKELAE
                     LSSVRDAMKVQWNNEKDRIDESRRFKEELEHLRTEEIRFTRDGNLTKAAEIKYGRIPE
                     LE"
BASE COUNT         1028 a         1004 c         1054 g         1134 t
ORIGIN      
        1 cgctttgacg tggaagcagt tcagggattt tcatcgaacc tactatcatc cggtaaactg
       61 taaggtcttt ctttcgggaa atattcccac cgaaagacaa cttgctcttt tgcaggaaga
      121 gtttctctgc cattttacct cagcccctgc tcctgctctg gtttctcctt cttctgatac
      181 cgactggcca aaggaatttg ttgtgcctgc gccctcagga gatggccaag atctttcttt
      241 ggtaactgcg caacttaatt ggcttttacc tgcggtaacc gatgtggtta cgtacatgga
      301 agcaaacctt cttaccgaaa tattgctggg ccatgacggg tcccccctga atcgggtttt
      361 acttgaatcc gctcttggag aggatgttgc cccttcaagc ggtattgaga ccgaggttct
      421 tcacctctgt tttagtgttg gacttagggg tatggcaaaa gatgatgtgc ctgcctttga
      481 acaacttgta ctggataccc tgggcgacat tgcggcacat ggtgtgagct cagaagaact
      541 ggagagcgcg gtacgatcca ttgatttttc caatcgggaa gtcaggcgtt caggtgggcc
      601 tttttcgctt acgctgatgc gccgatcatt acgcggttgg attcacggag tcggccctta
      661 tgagaccctt cgcgtaattc ctgcattcga agaagttaag acaaggcttg ccaacaagag
      721 cgactatatt caatctcttg ttcgccgctt ccttcttgat aatcctcgcc gcgctcgtgt
      781 agtggtttac cctgattcac aatggattgc ccgcatggat gagcgcgtag atgcacgagt
      841 caagcttttt gaatccactc tttccgggga agaggaacag gcgtttctct ctcgtcagga
      901 agacttattt gcccgtcagc gcgaagcgga cagcgaggag caacttgcgc ttattcctca
      961 tctgagccgt tcggatttac ctcctgtagc tgattcaatt ccatcacgaa taactacctg
     1021 tggaaaggtt ccgattgtct tgcatgaaca gccggtaaac aagattatct atgtggatct
     1081 tgcagtgccg attgacgtta ttaaaacatc agattatccc cttcttcctt ttttctcggc
     1141 cgttcttacc agcatggctg ttgatggtct ttcatgggtg gaggcttctt tgttaacggc
     1201 ccgctatacc gcatctcttg gagctatgct tttttcaagt cagatggtta gcggctatca
     1261 ggtcccgcaa tcccttgagt ttgtaaaggg aggcagggac tttttaatga tccggatgaa
     1321 ggctcttgag gaactgtctt ccgaagcgat ttctcttatg ttccgctttt ttgaaagagc
     1381 tgatttttcc gatacaaagc ggcttcttga tttactgaat gaataccgaa acgatcttga
     1441 atcttctctt gctcctgcgg gtaatcaata tgccgtctct cgggctgcag cctttgccag
     1501 tcgaaccaag gctgttgacg aaatatggaa tggattgacg cagctccggt ttatacggga
     1561 actgtgcgcg caagctaagg atacaaaggc tcttgtttct ttggctgagc gattgacttc
     1621 cattgcacag caattacgat cgtccgggat tgtggtaaat gccaccgccg aagcgacgag
     1681 tatggcttcc cttcaggctc aattatctcc acatctagaa ggctatgacg ggccagctcc
     1741 gtataccccg gatcaaaaac gggccagtga gctttattgc tgtatcgagc ccgagttttc
     1801 cggcagtttc cgccttttaa tcagttctcc cttgcaggtg ggctttgcag ccgcggttct
     1861 tccgtcagtt ccctatacac accccgatca tccggtagag attgttttgg ggcattggct
     1921 ttccagtgga ccgctatggg agcgtatccg gaccactgga ggtgcctacg gagctttttc
     1981 ctatccggat tctctggagg atttgttcgt catggcaacc tatcgcgatc ctcaacctct
     2041 tgcatccctg gcggttttta aggaagcact tgaagaagca tctttacatc ccatcgagtc
     2101 aaaggccctg gaccggatcg ttaccggagc ctatagccgg gaaattcagc ctcgctcccc
     2161 ttcggacaag ggctttactg cttttatccg tcttctctat ggaattaccg atgaagcgag
     2221 aaaaacaaag atagccggaa ttcttgcggt tacccccgct gagctttctg cctgcgcaac
     2281 caggcttctt gactcatggc cttcggtttg ccaggctgtt tttgcggggg aaaagatgct
     2341 taaagacggt gaaaacgatc gttttgctgg caaagtggct aaaatgacta tataattcat
     2401 ctggaagcac tacaggaggc gacacatcat gaaaaaaatt gataaggaag gatatcacat
     2461 actcaagcaa ctggtgtctg acgttcaggg ggcgccgtat cctaatgtaa tcgataatga
     2521 gttatatcga atatggtatg aacatgcgca ggttgttgcc attcaatgtc ttgaatatat
     2581 cgataaaaac tttcccaagg acaaggattc cgaaaaactc ccttcttttt aagcgtctgt
     2641 agatgcagag aatcatggac ggatgagctc cttgtgagta tatttatcac atgaatctcg
     2701 atacatgcac cattaaaaca cgtgaggcga tcggggaagc atcttcgatc gctcaacggc
     2761 gcgatcatgc gcagatagaa accgaccatc tgttattcgc actactttct caagaaggtg
     2821 gcattgtacc gcccctggta gaaagaattg gcatctcggc gagcgctctg cttaaagagc
     2881 tttctgaccg gcttgatcgg cttcctcggg taagtggaga ggccgcccag gtccattttt
     2941 catctcaggc ttccaaagtt cttgccaagg ccgaacaaga ggcctcatcc ctaaaagacg
     3001 aatacgtatc aaccgagcat cttttattat ccattgcggc ctctgaggat caagctggca
     3061 aatttttaca agaaaagggt attacccgtt ctgccctttt gtccgcgctc aaagaggtcc
     3121 ggggaaatca acggataacc acagaggatc ccgaagcgac ctttcagagt ctggagaagt
     3181 attgccggga tttaaccgcc cttgcacgtc aggaaaagat tgatccagtt attggcaggg
     3241 atgaagaaat caggcgggtt atgcaggttc tttctcgtag aaccaagaat aacccggttt
     3301 taatcggtga gcctggtgtt ggaaaaaccg ccattgttga aggccttgcc cggcgtattg
     3361 tttccgggga tgtgccggac agccttaagg gaaaaaagct tctctccctt gatttgggtg
     3421 ccctggttgc cggagcaaaa tttcgcggtg aattcgaaga gcggcttaaa gccgttattc
     3481 acgaggttca aaaagcggaa ggttcaataa tcctgtttat cgatgaactt catacccttg
     3541 tgggggctgg cgcaagcgag ggatcaatgg atgcctcaaa tttgctcaag ccagcgcttg
     3601 cccgcggaga gcttagagcc attggggcca ccacattaaa cgaatatcgc aaatacatcg
     3661 aaaaagacgc ggctcttgaa cgacgctttc agcaggtgta ttgtcccgaa ccaagcattg
     3721 aagataccat ttccatatta cggggacttc aggaaaagta tgaggttcat catggggtac
     3781 gcattcgcga tgacgccctt gttgctgcgg caaccctgtc cgatcgttat ataacgagtc
     3841 gtttcttgcc ggataaggca attgatctgg ttgatgaggc ggcaagtcgg ctcaagatgg
     3901 aaattgagag tcaacccaca gagctcgaca aggtggagcg caagattctt caatacaata
     3961 ttgaaaaggt gtctctttcc aaagagcggg acactgcctc cgtggaacgc cttgcaaagt
     4021 tggaaaagga acttgccgag ttatcttctg ttcgcgacgc aatgaaggtt cagtggaata
     4081 acgagaaaga ccgcattgac gagtcgcgcc gttttaaaga agagctggaa catctaagaa
     4141 ccgaggaaat tcgctttacg cgcgatggaa accttacaaa ggccgcagaa atcaaatatg
     4201 gaagaatccc cgaacttgag
//
LOCUS       DAOQXQ010000053         4174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_21755_length_4174_cov_3.156834, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000053 DAOQXQ010000000
VERSION     DAOQXQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21755_length_4174_cov_3.156834"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKH81_01490"
     CDS             complement(<1..682)
                     /locus_tag="PKH81_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96739.1"
                     /translation="MLYVYIELRYQRQLTRICPDVPVAFFSSFGEAVRKNGGVVVRMT
                     NGNLYQFDDGSVGYAFSASRALEELRILLQENRQRIREYRIIVESSPYRVSPDSFRDS
                     LQVCQTMILPDEGILLTQSALEILSSYISVVPCPDTPLFLFTALRISEYTQSSVNESS
                     VYPAFPLWLSSESGLGDTISVLRDLVFSVESTMQGVEIATEGSQALDVHAMYRFSSEH
                     PAYRTKAAL"
     gene            799..1737
                     /gene="argF"
                     /locus_tag="PKH81_01495"
     CDS             799..1737
                     /gene="argF"
                     /locus_tag="PKH81_01495"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ96740.1"
                     /translation="MKPTSLKGRSLLSWKDWTAEEILFLLELSHSVKKAARNRDIHQR
                     FTGKTLAMIFEKRSTRTRCAFETAFGEEGGHPVFLSTQDIQLGAKESIEDTARVLGRM
                     FSAIEFRGFKQETVDILAQESGVPVYNGLTDQFHPTQVLADIMTLQETFGKAQGRTIC
                     YVGDGRNNVARSLIVISAKLGINCTIVCPKELTCDEKLLAEARSWASDSGSKITVTDS
                     LDGVVGADVVYTDVWVSMGEEAKKEERHALLRPYQVNAELMRKTANPECIFLHCLPAV
                     KGEEVTIEVIEGPKSRVWDQAENRKHTIKAIMLATI"
     gene            1778..2185
                     /locus_tag="PKH81_01500"
     CDS             1778..2185
                     /locus_tag="PKH81_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96741.1"
                     /translation="MKKSSLAIIALTLILGLFAVVPAVAADSDLYYVNVPILKIFPHK
                     LGYYVIYRRAGLKTGEIYIPHAWFDRRDSRAILNLTEQNVTTYLTIVTRNGEFDHIRV
                     VAKKNIQHNTWGTIGQNALNADRFNVEKLNVEY"
     gene            2211..3374
                     /locus_tag="PKH81_01505"
     CDS             2211..3374
                     /locus_tag="PKH81_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ96742.1"
                     /translation="MVQAIREEHLDGWLFYNLAHRDPLTDTILDLDIHSPATRPWVYA
                     VLSNGNHVKIVSTLEPGILDSLPGERYTYQTRDEWIAHLSRFSHLSMAILSDEALPSL
                     STLPDSFATTLRNLGTKLVSAATAIQRAFSILDTKGIESHEVAAHQLYAIVENTAMHL
                     CRSYADGKPLTEGDIQDYILEHTRAGNLESSDSPIVAAGKNSANPHYEVPGHPFSGPR
                     GKVLCEGDVLQIDLWACQKGGIYADISWVFVYGTMSRPELDEAFSAVSSARDMALDFI
                     LSRCTLQEPITGYEVDAKVREFLLSKAPQWALRHRTGHSIDRFCHGLGANLDSTEFPD
                     SRYLLEGSLFSVEPGLYYQDFGFRTEINVYMSEHRPIISGGLPQERIITMKDR"
     gene            3378..>4174
                     /locus_tag="PKH81_01510"
     CDS             3378..>4174
                     /locus_tag="PKH81_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ96743.1"
                     /translation="MKAVPQELRDLILNHDFFIIAGHKEPDGDCVGSCLALASYLERK
                     GKKAVLLSAGPFKRVEIRQFETFFSDSIAGTSVPDSAVSLILDCSDLQRTGDAGIGLE
                     AYPQAVIDHHATNGSSGPADFVDSSVPATTLLVLALIESCGDTPNLVEAEHLLFGMCT
                     DTGFFRHLDASSAETFQAVSRLVTCGANPKKTFARMNGGKSFGSRILISRILSRMQRF
                     YNGQLVISWENLEETEEFGMEGRDSDSLYQLIQSISGVEAIVIVRQET"
BASE COUNT         1129 a          993 c          994 g         1058 t
ORIGIN      
        1 ccagggctgc cttggttcga tacgctgggt gttcagaaga aaaccgatac atggcgtgaa
       61 catccagggc ttgagaacct tcagtagcta tttcgactcc ttgcatagtc gattcaactg
      121 aaaaaaccaa atctctcaat acagaaatcg tatccccaag tccagactca gaggaaagcc
      181 atagcggaaa tgcgggatac actgagcttt cgtttacaga gctttgtgtg tattcgctaa
      241 tgcgcagtgc ggtaaaaaga aacaggggtg tatccgggca tggaacaaca gagatatagg
      301 acgacagaat ttccaaagcc gactgagtga gtaaaattcc ctcgtcggga agtatcatgg
      361 tctggcatac ctgcaagcta tcccgaaaac tatctggaga cacacggtag ggagaagatt
      421 caacgataat gcgatactcg cgaattcgct gacggttttc ttgtaatagt attcgcaact
      481 cttcaagagc gcgagaagcg gaaaacgcat aaccaaccga tccatcgtca aattggtaga
      541 gattcccatt tgtcatgcgt accactacac cgccattttt ccgtaccgct tccccgaacg
      601 aggaaaaaaa ggcaaccgga acatcggggc aaatgcgggt tagctgacgc tgataccgta
      661 gttcaatata cacgtaaagc atggttatcc cctgaatact cggaaaaaaa atgattgcat
      721 tgacagcgtt tccgcaggtt gttagtataa acccgttaac gggaaaaatc gagttgtttt
      781 tgagacaagg ggaaatccat gaaaccgaca tccttaaagg ggagatcact gctgagctgg
      841 aaggactgga ctgccgagga aattcttttt cttctggagc tctctcattc ggtaaaaaag
      901 gcggcacgca accgggatat ccatcaacgt tttaccggaa aaacacttgc catgattttt
      961 gaaaaacgct ctacccgtac ccgctgcgca tttgagacag cctttgggga agaaggcggt
     1021 catcctgtat ttctgtcaac acaagatatt cagcttggcg ccaaggaatc gatagaagat
     1081 accgcacgag tccttggacg tatgttttct gcaattgaat tcaggggctt taagcaggaa
     1141 acggttgata tcctggcaca agaatccgga gttcctgtgt ataatggatt aaccgatcag
     1201 tttcacccca cccaggtgct cgccgatatc atgaccttac aagaaacgtt tggtaaggcc
     1261 caaggaagaa caatctgcta tgtgggagac ggacgcaata atgtggctcg ctctcttatc
     1321 gttatctcgg caaaactggg tatcaattgc acgatagtgt gtcccaaaga acttacctgc
     1381 gacgaaaagc tgcttgccga agcacgctcc tgggcttcag actctggttc aaaaataact
     1441 gttaccgaca gccttgatgg ggttgttggc gcggatgtgg tatataccga tgtctgggta
     1501 tctatgggag aagaagcaaa aaaggaagag cggcacgccc ttttgcgccc ctaccaggta
     1561 aacgcggagt taatgcgcaa aacagccaat cctgaatgca tctttttgca ttgtttacct
     1621 gccgtaaagg gcgaagaagt taccatagag gtcatagaag gcccaaaaag cagggtttgg
     1681 gaccaggcag agaaccgcaa acacacaatc aaggcaatta tgcttgcaac aatctaaaag
     1741 caggatatat aattaagtac attccggagg ttgaatcatg aagaaatcct ctctcgctat
     1801 tatcgctttg actcttattc ttggtctttt tgcagttgtg cccgctgtgg cagcagactc
     1861 ggacctgtat tacgttaacg tgcctattct caagatattc ccccacaaac tcgggtacta
     1921 tgttatttac cgtcgggcgg gtcttaaaac tggggagatt tatatccccc atgcatggtt
     1981 tgaccgccgc gatagtcgcg ccatccttaa tcttaccgag caaaatgtaa cgacttacct
     2041 caccatcgtt acccgtaatg gtgagtttga ccacatcaga gtggttgcca aaaagaacat
     2101 tcagcacaac acttggggaa ccatcggtca aaatgcccta aacgctgaca ggttcaacgt
     2161 tgaaaagctc aatgtagagt attaatttct gcatgtgtaa tcatcaatcg atggtacagg
     2221 ccatccggga ggaacacctc gacggatggc ttttttataa tctggcccat agagaccccc
     2281 tgacagatac aatcctggac ctggacatcc acagccctgc aacccgcccg tgggtatatg
     2341 ccgttctttc caatggtaat catgttaaaa tcgtgtccac cctggaacca ggcatcctgg
     2401 attctcttcc gggagaaagg tacacctacc aaaccaggga tgaatggata gcccatctgt
     2461 ctcgtttttc gcacctatcc atggcgattc tttccgatga agcccttcct tccctttcga
     2521 ctcttccaga ttcttttgcc acaaccttac gaaaccttgg tacgaaactc gtatctgcgg
     2581 cgacggcaat tcaacgagct ttttccatat tggatactaa gggaatagaa agccatgaag
     2641 tagccgctca tcaactctat gcaatcgttg aaaacactgc tatgcatctg tgtcgctctt
     2701 acgctgacgg caaacccctt accgagggag acattcagga ttacatcctt gaacacacac
     2761 gagcggggaa ccttgaatca agtgacagtc caattgttgc cgccggtaag aacagcgcaa
     2821 acccacacta cgaggtacct ggccacccct tttccgggcc aaggggaaag gttctctgcg
     2881 aaggagatgt acttcagata gacctgtggg cttgccaaaa aggaggaatc tatgccgata
     2941 tctcctgggt gtttgtatat ggaacgatga gtagacccga gctggatgag gccttttctg
     3001 cagttagttc ggcacgagat atggcgcttg attttatcct tagccggtgt acactacaag
     3061 agccgataac tggatacgag gtcgacgcaa aggtcaggga gtttcttctt tcaaaagccc
     3121 cgcaatgggc ccttcgacat cgaaccggtc attccatcga ccgtttttgc catggtcttg
     3181 gcgcgaatct tgactccacc gaatttccgg atagccggta tcttttggaa ggaagtcttt
     3241 tttcggttga acctgggtta tattatcaag attttggttt ccggacagaa atcaacgtct
     3301 atatgagcga acatcgtcca attatatctg ggggtctccc ccaagaacgc attatcacta
     3361 tgaaggatag gtaaaaaatg aaggcagttc cccaggaatt acgcgacctg atactgaatc
     3421 acgatttttt tattatagca ggacacaagg aaccggatgg cgactgcgtg ggtagttgcc
     3481 ttgccctggc ttcgtacctg gaacgaaaag gaaaaaaagc ggtattactg tctgccgggc
     3541 cctttaaacg agttgaaata cgacaatttg aaactttttt tagtgacagt attgctggca
     3601 cctctgtccc ggactctgca gtgtccctta ttttggactg ttctgacctg cagcggactg
     3661 gggatgcggg tattggactg gaagcctatc cacaagcagt tattgaccat catgcaacga
     3721 acggttcaag cggacctgca gattttgttg acagttcggt ccctgcgact accttgttgg
     3781 tattagcctt gatcgaatcc tgtggcgaca cccccaattt agtcgaggca gagcatctcc
     3841 tttttggcat gtgtaccgac accggttttt ttcgccatct ggacgctagc agtgcagaaa
     3901 cgtttcaggc ggtatcccgc ctggttacct gtggggccaa tcccaagaaa acctttgccc
     3961 gcatgaatgg gggaaaatcc tttggctccc gtattttgat ttcccgcatt ttatcaagga
     4021 tgcagcgttt ttataacggt caactcgtta tttcctggga aaaccttgaa gagaccgagg
     4081 aatttggcat ggaaggccgt gattctgatt ccctgtatca acttattcaa agcatcagcg
     4141 gagttgaagc aattgttatt gtccgtcagg aaac
//
LOCUS       DAOQXQ010000054         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_21906_length_4155_cov_2.677317, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000054 DAOQXQ010000000
VERSION     DAOQXQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21906_length_4155_cov_2.677317"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..>425)
                     /locus_tag="PKH81_01515"
     CDS             complement(63..>425)
                     /locus_tag="PKH81_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ96744.1"
                     /translation="PLHFYGTYAITKHTGEFIFSQGEDSDEIYYVWQGTIRLTLPNQI
                     MDVGRGKMFGEFSSIVGGTRTATAVALSDVLLLKIPGRVFQETLEVDGALSRKTLQMI
                     TDYVRKSNNLPLFSEDEL"
     assembly_gap    426..435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<436..1355)
                     /locus_tag="PKH81_01520"
     CDS             complement(<436..1355)
                     /locus_tag="PKH81_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNQ96745.1"
                     /translation="MRRRQPLIHSLVQRLYITWETFNGNDLFTYASAGAYSFLLSALP
                     MVLMVLVILIRFLHTSPQVIQELLAENTLIANSFDASSFIHSVISIKQIGLFEVVLAL
                     SVFWMARRFFMSIQQGMKIIYRKRGTKKAIKENLIIIAGEAILVILVVVMVIIITAGN
                     ALFHTMLPQNFLTPFVVTLFKNLFRFVPFGVIPIFLFLVYYFAPSARPPARNSWWASL
                     ACTASFAIVQAAFSAAINVSRYNLVYGILSNVIVILLEVYLFFFLFLFFAQYQYVTQF
                     YDSFLIAQLYLLPPYDDVRISRQIERTMFI"
     gene            1525..2412
                     /gene="dapA"
                     /locus_tag="PKH81_01525"
     CDS             1525..2412
                     /gene="dapA"
                     /locus_tag="PKH81_01525"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNQ96746.1"
                     /translation="MTKLRGAFTALVTPMKADGSVDYDGYRALIRFQLESGISGLVPL
                     GTTGETPTLDEDEEERLIDIAMEEAKGKVPVILGAGSNNTRDAVKYVKRAKDKGADYA
                     LVVTPYYNKPNDEGLYLHFAACAEVGIPIIVYNIAGRTGKNISTSLLVRIAELPNIAG
                     VKEASGDVTQMMEVIERLGRVKKNFTVLSGDDALCLGLVALGGDGVISVMSNIAPAEV
                     TAMTQAALAGDLETARKMHYTLLPAFRACFVETNPVPIKAAMNMKGLPAGTVRLPLAP
                     LAKEHEQLVRSAFSAAGIL"
     gene            2444..3535
                     /gene="asd"
                     /locus_tag="PKH81_01530"
     CDS             2444..3535
                     /gene="asd"
                     /locus_tag="PKH81_01530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ96747.1"
                     /translation="MSNKIPVGILGATGAVGQNYIYLLQNHPWFEVTYVAASPRSAGK
                     TYREVVASKWLLGANIPEAVANLVIEDANIVEKAKGRCRFVFSALELDSKDEIKALEN
                     AYAAMGFPVVSNASAHRWTDNVPMLIPEINADHISVIDSQKKAHGWDTGFVAVKPNCS
                     IQSYMIPVWALMQAGYEVKRMFVSTMQAVSGAGYPGVPSLDMIDNIVPFIGGEEEKTE
                     KEPSKIFGKVEGGKIVNFQGLKVSAHCNRVPVINGHTACISLEFGDKKPSLEEIDRIW
                     KSFRGLPQELDLPFAPVNPIIVRPEANRPQPRRDRDEDKGMAVSIGRLRTCELFDIRF
                     VALSHNTVRGAAGGGILNAELLKAKGYIQ"
     gene            3532..>4155
                     /locus_tag="PKH81_01535"
     CDS             3532..>4155
                     /locus_tag="PKH81_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNQ96748.1"
                     /translation="MSDKTFPLKLPELTALSAQYPTPFYLYDEKAIRDHAKRFVGAFK
                     SRFPSFREYFAVKACPNPFILKILASEGLGADCSSLPELILAQKAGITGENVMFTSNE
                     TPEKEYVYANLMSAIINLDDISHIDFLERHAGLPRLICFRYNPGPERTGNAIIGNPSD
                     AKYGLTKEQLFHAYQVCKEKGVKRFGLHTMVASNELQADYFVETARML"
BASE COUNT         1113 a         1041 c         1060 g          931 t
ORIGIN      
        1 ccgatataac cgacaattcc gcacataggc tctcctccgg tatcactcga cgaaaaaagc
       61 tatcatagtt catcttcact gaaaaggggg agattgttcg attttcgaac ataatcggta
      121 atcatttgca gggttttgcg ggaaagggct ccatctacct ccagggtttc ctgaaaaacc
      181 cttcccggaa tctttagaag caatacatcg ctcagggcta ccgccgttgc ggtgcgggtt
      241 cccccaacga tactggaaaa ctctccaaac atcttgccgc gccccacatc catgatttgg
      301 ttcggcaggg taaggcgaat agtcccctgc cacacataat agatctcgtc cgagtcctct
      361 ccctgactaa aaatgaattc acctgtatgt ttggttattg cgtatgttcc gtaaaaatga
      421 agcggnnnnn nnnnntctat aaacatagtc cgctcaattt gccggcttat acgtacatca
      481 tcgtagggag gcaggagata caactgggca atcaaaaagc tgtcgtaaaa ctgagtcaca
      541 tactgatatt gggcaaaaaa caagaataaa aagaaaaaga gatagacctc cagaaggata
      601 acaatcacgt tgcttaaaat tccgtacacc aaattgtatc gggatacgtt tatagcggcc
      661 gaaaaagcgg cttgtactat cgcgaaggat gctgtgcagg caagagatgc ccaccaactg
      721 ttacgggcgg gtggacgggc gcttggagca aagtaatata ccaaaaaaag aaaaatgggg
      781 attaccccaa agggaacaaa gcgaaagagg tttttgaaca gggtaaccac aaagggcgtt
      841 aaaaagtttt gggggagcat ggtgtggaac agggcattac cggcggtaat gataattacc
      901 ataacaacaa ccaaaataac caggatcgct tccccggcaa taatgatcaa gttttccttt
      961 atcgccttct tggttccccg tttgcgataa ataatcttca ttccttgttg gatggacata
     1021 aaaaaacgcc gtgccatcca aaagaccgac agagcaagca ctacttcaaa aagaccaatt
     1081 tgtttaatgg aaataaccga gtgtataaag gacgaagcgt cgaatgaatt ggctatcagg
     1141 gtgttttccg caaggagctc ctgaataacc tgtggcgagg tatgtaaaaa acggataaga
     1201 ataacaagga ccataagcac cattggcagg gcagacagaa ggaagctgta tgctccggcc
     1261 gacgcatagg taaaaaggtc gttcccgttg aacgtttccc aggtgatgta gagcctctgg
     1321 accaagctgt gaatcagtgg ttgtcttctc cgcatagggc atgtagtata ccgtagaccg
     1381 gtaaaaattt atagcaaaat accgggattt accgactttt accccctaag ctcttgaatt
     1441 tatgtataaa tatgtaataa actggtataa aattgcagtc gagggtgacc gtcaatggga
     1501 aaaccccgac caaggagagg ctttatgacc aaactgcgcg gagcgtttac cgcacttgtc
     1561 accccgatga aggccgacgg atcagtagat tacgacggat atcgggccct gatacggttt
     1621 cagctcgaaa gcggcatttc cggcctcgta cccctgggaa ctaccggtga aacaccgacc
     1681 ctggacgagg atgaggaaga gcggctgatt gacattgcga tggaagaggc aaagggaaag
     1741 gttcccgtta tcctcggtgc ggggagcaac aacacccgcg atgccgtcaa atatgtcaaa
     1801 cgcgctaaag ataagggggc tgactacgcg ttggtggtta ccccgtatta caacaaaccc
     1861 aatgatgagg gactgtacct tcactttgcc gcctgcgcgg aagtgggaat tcccatcatt
     1921 gtgtacaata ttgccggaag gaccggaaag aatatttcta cctccctgct ggtacgaatc
     1981 gcggagcttc ccaatatagc aggcgttaaa gaagcgtcgg gggatgttac ccaaatgatg
     2041 gaagtaatcg aacgccttgg acgggttaaa aagaacttta cggttctctc aggcgatgac
     2101 gcgctctgct tgggcctggt agctttgggc ggcgacgggg tgatatcggt tatgtccaat
     2161 atcgctccgg cggaggtaac ggcaatgact caggctgccc ttgcaggcga cctggagacg
     2221 gcgcgcaaga tgcattacac ccttcttccc gcattccgtg cctgttttgt ggaaaccaat
     2281 ccggttccca taaaggccgc catgaatatg aagggccttc ctgcgggaac agtgcgtctt
     2341 cccctggcgc cccttgccaa ggaacatgaa cagctggtcc gatccgcctt tagcgcggcc
     2401 ggtattttat aacaacctgc atacataaat ggagagaaca tcaatgagca acaagatacc
     2461 cgttggaatt ctcggcgcga cgggcgccgt tggacaaaat tatatatacc tcttgcaaaa
     2521 ccatccctgg ttcgaggtta cctatgttgc ggcgagcccc cgctctgccg gaaaaaccta
     2581 tcgcgaggta gtggccagca agtggctttt gggagcaaat attcccgaag cggtggctaa
     2641 cctggttatc gaggacgcga acatcgtgga aaaggccaag ggccgctgtc gcttcgtctt
     2701 tagcgccctt gagctcgaca gcaaggacga aatcaaggct ctggaaaatg cctacgcagc
     2761 catgggcttc ccggttgtat ccaatgcctc tgctcatcgc tggacggaca atgttcccat
     2821 gctgattccc gagattaacg ccgatcatat ttcggttatc gattcccaaa agaaggctca
     2881 tggctgggat accggttttg tggcggttaa gccaaattgc tccatccaaa gctacatgat
     2941 tcccgtatgg gccctaatgc aagccggcta cgaggttaag cgcatgtttg tctctaccat
     3001 gcaggctgta tccggtgcgg gttaccccgg cgtgccgagt ttggacatga tagacaacat
     3061 cgttccgttt attggcggcg aggaagaaaa gaccgagaag gaaccgtcca agatctttgg
     3121 caaggtagaa ggcggcaaga tagtcaactt ccagggcctt aaggtgagcg cccattgtaa
     3181 ccgcgtaccg gtaatcaacg gacacacggc ctgtatcagt cttgaatttg gcgacaagaa
     3241 gccttccctt gaggaaattg accggatatg gaagagcttc cggggcttac ctcaggaact
     3301 agatcttccc tttgcgccgg tgaacccgat catcgttcgc ccggaggcaa accgtcccca
     3361 gccccgccgc gaccgcgacg aggacaaggg tatggcggtt tccattggac ggctcaggac
     3421 atgcgagctc tttgatatcc gctttgtcgc gctttctcat aataccgtcc gcggagctgc
     3481 aggcggtggc atccttaacg ccgaacttct taaggcaaag ggctatattc aatgagcgac
     3541 aagacctttc cgcttaagct tcccgagtta accgccctga gcgcgcagta tccaacccct
     3601 ttttatctct atgatgaaaa ggcgatacgc gatcacgcca agcgatttgt gggggccttt
     3661 aagagccgat ttccctcctt tagggaatac ttcgccgtaa aggcctgccc caatcccttt
     3721 attctcaaga tactcgcgtc agagggcctt ggcgcagact gttcaagtct gcccgagctc
     3781 atccttgccc aaaaggcggg tattaccggc gagaacgtta tgtttacctc caacgaaacg
     3841 ccggaaaagg aatatgtcta cgccaacttg atgagcgcca ttatcaattt ggacgacata
     3901 agccacatcg acttcctgga aagacacgcc ggtctccccc gactcatctg ctttcgatac
     3961 aatccgggcc ctgaaagaac gggaaatgcc atcatcggta acccatcgga tgccaaatac
     4021 gggctcacga aggaacagct ttttcatgcc taccaggtct gcaaggagaa aggagtcaaa
     4081 cggttcgggc tgcacaccat ggtcgcctcg aacgagcttc aggccgatta cttcgtggag
     4141 acggcccgaa tgctt
//
LOCUS       DAOQXQ010000055         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22040_length_4134_cov_2.728610, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000055 DAOQXQ010000000
VERSION     DAOQXQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22040_length_4134_cov_2.728610"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKH81_01540"
     CDS             complement(<1..483)
                     /locus_tag="PKH81_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNQ96749.1"
                     /translation="MIHNSVLRIGIDVGSTTVKVVVLNADNTVLFSKYERHRADIRST
                     IISVVEQALTEIDTRYPSSSDVLLSAMVTGSGGLSVSRWLSIPFIQEVIAATTAVRKI
                     IPKTDVAIELGGEDAKITYFSDGVEQRMNGTCAGGTGAFIDQMAALLETDADGLNELA
                     R"
     gene            554..2122
                     /gene="murJ"
                     /locus_tag="PKH81_01545"
     CDS             554..2122
                     /gene="murJ"
                     /locus_tag="PKH81_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNQ96750.1"
                     /translation="MAHSIIKSGSRLSLLTMGSRILGLVREMTKAAFLGTSALSDAFT
                     IAFFIPNLLRRLFAENSISVAFIPTFRQYLDNESPQKIKEFLSATFTVIALATSLTVV
                     AGIIGAPLIIPFFGTGTDETVLLTRMMFPYLAIISLAAFFQGILNGIRVFSPTGFTPI
                     LFNLVIIGSTWLFSSFTGNPARAMAIGVLLGGTVQAAFQLPFVLKAGFRFKPVNPLVA
                     LSDPGTKTVLHLIGPTVIGMAAYQINDLVSSILASNAGTGIVSSLQYSLRLQELILGV
                     FAVSIGTVILPDLSSHAAKEQWDLFTRLLSRAMNIIAFITIPVTFFSLVFGQSLITLL
                     FKTRSFSDDSVALTLNAFTFHVMGLYCIALNRIISPAFYAKKDTKRPTIAGLFSFCAN
                     IILALLLVGPMRGGGIALALSLASAVNTACLLYFLGKTKTVELGPFLRNTASYAGKIT
                     LFSAVSACPLWYFKEYLLTPFANLGRIAGNAIPITLGFGVFLLIFVLQLCMTKDPVLT
                     SLRERLSGTKRSDY"
     gene            complement(2116..3483)
                     /locus_tag="PKH81_01550"
     CDS             complement(2116..3483)
                     /locus_tag="PKH81_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ96751.1"
                     /translation="MSLSFGKKLVYALGQFGCVLCAYGVGKLFLRFFAPGNADLFPAY
                     LPQGSFFGMFTIAGLIVALSSLAYVSSSLVSGWVSDKTCTLQGRRIPFLLSFAIPLSL
                     VSAFIFYPPGVPGSTINAVYVSILTLFFFVSLSFYAVPYLALLAEVGQSPRDRLQLSV
                     MLAFSTAFATLLGNRIYTLMDILVASTGMSALTAFRSVIFFFSAVSLSCLLAPVLAIK
                     REGLSDGEAITESFTTALSAVLGDKNFRSYVWAELFFRCAFATLMTGFSAHITVLLGL
                     PKRASDLFLLLLFFSNLALYVPLFYFVRRYGKRKILFIAFILLVLALSAGSFAGKYPA
                     GALVQGLILSILFSLSYAAFTAIPLALVADLSAANERKTGLSRAGVYFGLHACAVKGG
                     EILAGLLFPLIIVASGSSLLDTPRRFSLRLTLVLGGISAILGFLALFGYREKEVMSVL
                     ERPDQ"
     gene            3573..>4134
                     /locus_tag="PKH81_01555"
     CDS             3573..>4134
                     /locus_tag="PKH81_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNQ96752.1"
                     /translation="MQMIHQKRRADLVTWMARNSVSVVVIEDTEGRRDPALRYFSGHP
                     CDALLLITVTGHAVLCPWDNNLAEQRAQVDVIVPYSEFGRHPVTAVQLLTERIGVPPQ
                     SRIEIPSQTPYPLFLTYVEALFGYNVLCRKDGGAHEEIERMRSIKDLLEIEHIEKAAA
                     ITDTLIDQVEKQVRSGKIKTEIDAALF"
BASE COUNT         1105 a         1080 c          976 g          973 t
ORIGIN      
        1 tctggccagt tcattcaagc cgtcagcgtc agtctctaaa agggctgcca tctgatcgat
       61 aaaagcaccg gttccaccag cacaggtacc attcatgcgc tgttcgaccc catcggaaaa
      121 ataggtaatc ttggcgtctt cccctccaag ctcgatggca acatccgttt tagggattat
      181 cttgcgcacc gcggttgtcg cggcaatgac ctcttgtata aagggaatag aaagccagcg
      241 cgacaccgaa aggccaccgg aaccggttac catcgcgctg agtaatacgt cagatgaaga
      301 aggatagcgc gtatctattt ccgtaagagc ctgttccaca acagaaataa tcgtgctgcg
      361 gatgtctgct ctgtgtcgtt catacttgct gaacagaact gtgttgtcgg cgttcagaac
      421 cacaacctta acggtggtgg atccaacatc aatgccgatg cgtagtaccg aattgtgtat
      481 catgtggcca tgatgccata gaaacaaggt caattcaaga cacagaaagc tttgtcatgc
      541 tatgcttatg agcatggcac attcaatcat caaaagcggt tcccgactgt ccctgttaac
      601 catgggttcg cgaatcctcg gattggttcg agaaatgacc aaagcggcct ttttgggaac
      661 ctccgcactg tccgatgcct ttactatcgc cttctttatc ccgaatctct tgcggcgcct
      721 ttttgccgaa aacagcattt ctgtcgcctt tataccgacc tttcgccaat atctggacaa
      781 cgaatctccg caaaaaatca aggaattcct ttccgctact tttactgtta tcgccctggc
      841 aaccagcctg accgtagtag ccggcattat cggcgctccc ctgattattc ctttttttgg
      901 caccgggacc gatgaaacag tcctattaac ccggatgatg tttccgtatt tagcgataat
      961 ctcccttgcg gccttttttc agggcatctt gaacggaatt cgcgtttttt ccccaaccgg
     1021 attcacgccc atccttttta accttgttat catcggttca acctggcttt tttcctcttt
     1081 taccggtaat ccggcaaggg ccatggccat tggagtgctt ttaggcggta cggttcaagc
     1141 agccttccag ctcccctttg tactcaaggc ggggttccgc ttcaaaccag tgaatcccct
     1201 ggtggccctc tcagacccgg gcacaaaaac cgtcctacat ttgatcggtc caacggtcat
     1261 tggtatggcg gcctatcaga ttaacgatct ggtttcctca attttggcaa gcaacgcggg
     1321 gacgggcatt gtctcttccc ttcaatattc tctgcgcctt caggaactta tcctgggtgt
     1381 cttcgccgta tccatcggaa cggttatcct tcccgacctt tcaagtcacg ccgcaaagga
     1441 acaatgggac ctgtttaccc gcttgctttc gcgcgccatg aacatcattg cctttatcac
     1501 aatccctgtt actttttttt ccctggtttt tggccaatcc ctgattaccc tgctttttaa
     1561 aaccagaagt ttttccgacg actcggtagc actgaccctt aatgccttta ccttccatgt
     1621 tatgggactc tattgcatcg ccttaaaccg gattatttct cccgcctttt acgccaaaaa
     1681 agatacgaaa agacctacca ttgccggtct tttttccttt tgtgcaaata tcatccttgc
     1741 cctgctgctc gtcggcccta tgcgcggggg cggcatagcc cttgccctta gccttgcaag
     1801 cgccgtcaat accgcatgct tgctctattt cctgggtaaa acaaaaacag tcgaattagg
     1861 gcctttttta cggaacacag ccagctatgc gggcaaaatc acccttttct cggcagtttc
     1921 agcctgtccg ctgtggtatt ttaaggaata tctactaaca ccctttgcca atctgggaag
     1981 aatcgccggt aacgcaatac cgatcactct ggggtttggt gtttttttac taatctttgt
     2041 tcttcaatta tgtatgacaa aggatccagt gcttaccagc cttcgcgaac gcctgagcgg
     2101 aacgaagcgc tccgattatt gatcagggcg ctccaaaaca ctcataacct ctttttcccg
     2161 atacccgaac agggctagaa acccgagaat tgcggaaatt ccccctaaca caagggttaa
     2221 ccgcaaagaa aatcgtcggg gggtatccag aagcgatgaa ccgcttgcga caatgatgag
     2281 cggaaacaac aatcctgcaa ggatctctcc tccctttacc gcacaggcat gaaggccgaa
     2341 atacactcct gcccggctca ggccggtttt tcgctcgtta gccgccgaaa ggtctgcgac
     2401 aagggcaagg ggaatcgcgg taaaggccgc gtacgaaagg gaaaacaaga ttgaaaggat
     2461 aagtccctga accagagctc cagcggggta ttttccggca aaagatcctg cgctcagggc
     2521 cagaaccagc aggataaaag cgataaacaa aattttgcgt ttgccgtatc gtctgacaaa
     2581 ataaaacagg ggtacgtata aggctaaatt cgagaaaaaa agcagtaaca aaaaaagatc
     2641 ggaagcccgc ttgggtaatc ccagaagcac cgtaatatga gcggaaaaac cggtcattaa
     2701 ggttgcaaag gcacagcgga aaaaaagttc cgcccacaca taggagcgaa aatttttatc
     2761 cccaagcacg gctgaaagcg cggtagtaaa cgattcggta atcgcctccc cgtcggaaag
     2821 gccctctcgc ttaatggcaa gaaccggcgc taacaggcag gagagcgaca ccgccgagaa
     2881 aaaaaagata acggagcgga aggcggtaag ggcgctcatg cccgtactgg caacaaggat
     2941 atccataagg gtgtaaatgc ggttacccaa aagggtcgcg aaagcggttg aaaaagcgag
     3001 catgaccgac aattgcaggc gatcgcgagg gctttgtcca acttccgcaa ggagggccag
     3061 atacggtacg gcataaaaag aaagactgac aaagaaaaaa agcgtaagaa tgcttacata
     3121 cacggcgttg atggtgcttc cgggtacgcc gggaggataa aaaataaagg cggaaacaag
     3181 ggataaggga atggcaaagg aaagcaaaaa ggggatgcgt cttccctgga gcgtacaggt
     3241 cttatcggac acccacccgg agactaggga tgaggatacg taagcgaggc tggaaagcgc
     3301 gacaataagg ccggcaatgg taaacatgcc aaaaaaggat ccttggggaa gatacgccgg
     3361 aaacagatca gcgttgcccg gagcaaagaa gcgtaaaaaa agcttaccca ctccatacgc
     3421 gcacaagacg cacccgaatt gaccgagcgc atacactaat tttttcccga acgacagact
     3481 catgcaccgc tccctgaaat acaaaatttt gtgagcaatg atagccgatt tgctttaccc
     3541 tgtcaaagaa attggagtat attggcactc atatgcagat gatacatcaa aagcgcagag
     3601 cagaccttgt aacctggatg gcgagaaaca gcgtatccgt agtagttatt gaagataccg
     3661 aaggcaggag agatcccgcg cttcgctatt tttccggtca tccctgcgac gcactcttac
     3721 tgataaccgt aaccggtcac gccgtcctct gtccgtggga caataacctt gccgagcagc
     3781 gtgcgcaggt ggatgttatc gtgccttaca gcgagtttgg ccgacatccg gttactgctg
     3841 tccagctcct taccgagcgc attggagtgc ctccacagtc tcgcatagag ataccgtctc
     3901 aaacccccta tcctcttttt cttacctatg tagaagccct ttttggatat aacgtactgt
     3961 gtcgcaagga cgggggcgcg cacgaagaga tagaacggat gagatccatc aaggacctgt
     4021 tggagattga acacatagaa aaagcggcgg ctattaccga cacccttatc gatcaggtag
     4081 aaaaacaagt tcgctcaggc aaaatcaaaa ccgagattga cgcagccctc tttc
//
LOCUS       DAOQXQ010000056         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22046_length_4133_cov_3.124080, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000056 DAOQXQ010000000
VERSION     DAOQXQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22046_length_4133_cov_3.124080"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKH81_01560"
     CDS             <1..1310
                     /locus_tag="PKH81_01560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNQ96753.1"
                     /translation="DKNRLESALKSQKRIGEVLVEEKIVSPADLKIALEEQKQLQRIQ
                     KNRAAQTDLSTIRVKSEKLDQLMALVGELVTVHARILQTTRVVSDNEELVSVVEQFGR
                     LTDELRNNTMSIRMVPIGTTFSSFKRLVRDLSSDLGKNVELVTEGGETELDKTVIEKL
                     NDPLIHIIRNSLDHGIEQPDARIAKGKDGTGTIRLRASQTGASVYIQIEDDGKGLDVE
                     AIRKKAIERGLINPDEVLSEKDVYKLIFAPGFSTNEVVTEVSGRGVGMDVVNRQMELI
                     GGTVDLESEPGVYTQVTLKIPLTLAIIDGLLVQIGEESYVIPLASVVGCLEFIQAKQK
                     NENRIVIFHDKQLPFINMRDFFDISGTRRAIEQMVVVNVKDQHVGILVDQVVGSIQTV
                     IKPIGKIFKRADGVASASILGDGSVALILDVERVLHASERTGS"
     gene            1307..2134
                     /locus_tag="PKH81_01565"
     CDS             1307..2134
                     /locus_tag="PKH81_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNQ96754.1"
                     /translation="MTGDTFKRFSEYIESTVGIRMPQTKEVMLEARLSKRVRALGFST
                     HEEYCDYVFSPEGHDREIQQLIDVVTTNETEFFREAKHYSLLSERVLPELVGDARSID
                     LNLWSVAASTGQEAYTLAIIVEEFSRVQNVKINYRILGTDISEKVLRVAETGVYTERQ
                     ASKIPLEYKRRYFLKSKNTAEETVRVRPEIRSKVVFRKLNLMDDLYPVTKKYHIVFCR
                     NVFIYFDRETQHKVLARIYSHIIPGGFLFMGHSENIGRSDLPLVSIASAVYRKENRT"
     gene            2131..3198
                     /locus_tag="PKH81_01570"
     CDS             2131..3198
                     /locus_tag="PKH81_01570"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNQ96755.1"
                     /translation="MINVMIIDDSAVVRQTLSDVLSSEDDIVVVETAADPYDAAEKLK
                     HLVPDVIILDIEMPRMDGLTFLRKLMSQHPLPVIICSSKAEAGSNNVFQAMQFGAVDI
                     IQKPKMGTRQFIEESRVMISDAIRAAHMTHVKKLTPSRPLASPKLTADVILPPVSAHH
                     VSIETTETVVVIGASTGGTEALRVFLEKLPVDCPGIVIVQHMPEHFTQAFATRLDSIC
                     EVSIKEAEDNDSVLRGRVLIAPGNRHLLLKRSGARYYVEIKDGPLVSRHRPSVDVLFR
                     ATARYAGKNAIGVIMTGMGDDGAKGMREMKDAGAFTIAQDEASCVVYGMPKEAVKAGA
                     VDVTVPLEAIADLVIKKGAMK"
     gene            3278..3643
                     /locus_tag="PKH81_01575"
     CDS             3278..3643
                     /locus_tag="PKH81_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ96756.1"
                     /translation="MGSTILVVDDSVSIRQSVRFILEQSGYTVHEAADGAEGLKRLGE
                     QKVDLVISDINMPNMDGLTMIKQIRETDKIKFLPILVLTTESQGTVIEEGKKAGATGW
                     IVKPFNDVKLIETIKKVLP"
     gene            3700..>4133
                     /locus_tag="PKH81_01580"
     CDS             3700..>4133
                     /locus_tag="PKH81_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNQ96757.1"
                     /translation="MTGEDIVISTLLGSCIAVALYDLEKAIGGLNHFMLPFTRNLDAH
                     KPEDSARYGINSMEMLINGLLKMGARREKLRSKVFGGSSVLRYQQEATYNIPKMNIEF
                     IFNFLDIEKIPIESYSVGGPFPRKIYYFPRTAKVLMRYSKQDS"
BASE COUNT         1166 a          811 c         1046 g         1110 t
ORIGIN      
        1 tagacaagaa tcgtcttgaa tcagccttaa agagccagaa gcgtataggg gaggttttag
       61 tggaagaaaa aatcgtaagc cccgcagacc tcaaaatagc attggaagaa caaaagcagc
      121 ttcagagaat acaaaaaaac agagctgctc aaacggatct aagtacaatc cgtgtaaagt
      181 cagaaaaact tgatcagctg atggcattgg ttggagagct tgtcaccgta catgcaagaa
      241 tactgcaaac aacccgtgtg gtgtctgata atgaagagct tgtttctgtt gttgaacagt
      301 ttggccgcct gacggatgaa ttaagaaaca acaccatgag tattcgtatg gtacccattg
      361 ggactacctt ctcgagtttt aaacgtttag taagagattt atcttctgat cttggtaaaa
      421 atgttgaatt ggtgacggag ggtggtgaga ccgagctgga taaaacagta attgaaaagc
      481 tcaatgatcc tttaattcac attatcagaa acagccttga tcatggtatt gaacaacccg
      541 acgcgcgcat cgcgaaggga aaggatggaa cggggacgat cagacttcgc gcatcgcaaa
      601 caggtgcttc tgtatatatc cagattgagg atgacggaaa aggtttggat gttgaggcga
      661 ttcgcaaaaa ggccattgaa cgtggtctaa ttaacccgga tgaggtgtta agcgaaaaag
      721 atgtgtataa attaattttt gcaccgggtt tttccaccaa tgaagttgta accgaggttt
      781 ctggacgggg cgttggaatg gatgttgtca atcggcagat ggagttaatt gggggaacgg
      841 ttgatttgga aagtgaaccc ggtgtgtata cacaggttac cttaaaaatc ccgttaaccc
      901 ttgctataat tgatggactt cttgtgcaga ttggcgagga atcctatgtt attccgcttg
      961 cttcggttgt tggctgtctt gagtttattc aagcaaaaca aaaaaatgaa aacaggatag
     1021 ttatttttca cgataagcaa cttcctttta ttaacatgcg cgactttttt gatataagcg
     1081 ggactcgcag ggcaattgag cagatggtcg tagttaatgt taaggatcaa catgttggca
     1141 tcttggtgga ccaagtagtg ggcagcatcc agacagttat taagccgata gggaaaatct
     1201 tcaaaagggc tgatggtgtc gcaagcgctt ccatattagg cgatggatcg gtggccctga
     1261 tcctggatgt ggagcgagtc ttgcatgcct cggagcgaac aggttcatga caggtgacac
     1321 ctttaagagg tttagcgagt atatagaatc cactgttggc atccgcatgc ctcaaacgaa
     1381 agaagtaatg ttggaagccc gcctttccaa gcgggttcga gctcttggtt tttctactca
     1441 tgaggaatat tgcgactatg tctttagccc ggaaggacac gatcgtgaaa tccaacaact
     1501 tattgatgtt gttactacaa acgagacgga attttttagg gaagcgaagc attattcgct
     1561 tttatccgag cgagtcttgc ccgaacttgt tggagacgcc cgatcgattg atttgaacct
     1621 gtggtcggta gcggcgtcta cgggacaaga agcgtataca ctggcgataa ttgtagagga
     1681 gttctctcgt gttcaaaatg taaagataaa ctaccgtatc cttggcactg atatatccga
     1741 aaaggtctta cgtgttgccg aaaccggtgt gtatacggag cggcaagcgt ccaagattcc
     1801 cttagagtat aagaggcgat attttctaaa aagcaaaaat accgcagaag aaacagtacg
     1861 cgtacgtccg gagattcgta gcaaggttgt ttttagaaaa cttaatctga tggacgatct
     1921 ttatccggta acaaaaaagt atcatatagt attctgccgg aacgtgttta tttattttga
     1981 cagggagacc cagcacaagg tgctcgctcg aatctattcc cacattattc cgggtggttt
     2041 tctttttatg gggcattcag agaatatcgg gcgaagcgat ttaccgttag tctcgattgc
     2101 ttccgcggtg taccgaaagg agaatcgcac atgatcaacg tgatgattat tgatgactct
     2161 gccgtggtac gacaaacact gtcggatgtg ttgtcttccg aagatgatat tgttgttgtg
     2221 gagaccgctg ccgatccata cgatgctgca gagaagctta aacatcttgt gcctgatgtt
     2281 attattctgg atatagaaat gccccgaatg gatggcctta ccttcttgag aaaactgatg
     2341 agtcaacatc ctctgccggt aataatttgc tcaagcaagg cagaggcagg atcgaacaat
     2401 gtattccagg ccatgcagtt cggcgccgta gatattattc aaaaaccaaa aatgggaacg
     2461 cgccagttca tagaagaatc ccgtgtcatg atatccgatg caatacgcgc cgcacatatg
     2521 acacatgtca agaaattgac gccctctcga ccactggctt ctcccaaatt aactgctgat
     2581 gtaattttgc cccccgtgtc cgcccaccat gtatcgattg aaactaccga gacagtcgta
     2641 gttattggcg cctctaccgg aggcacagag gccttgcggg tgtttttgga aaaacttccc
     2701 gttgactgtc cgggcattgt tattgttcag catatgcccg agcattttac tcaagccttt
     2761 gcaactcgac ttgatagtat ttgcgaggtg agcattaagg aagcagagga taatgattcg
     2821 gttcttcgcg gccgcgtatt gattgccccc ggaaatcggc atttattgct taagcgctca
     2881 ggcgcccgct attatgtaga gataaaagat gggcctttgg tgtccagaca tagaccctca
     2941 gtagatgttt tgttcagagc tactgctcgc tatgcaggca aaaacgccat cggcgtaatc
     3001 atgaccggga tgggagatga cggggcaaag ggcatgcggg agatgaaaga cgccggagct
     3061 tttactattg cccaggacga agcgagttgt gtggtctatg gaatgcccaa ggaagcggta
     3121 aaggccgggg cggttgatgt aaccgttccc ttggaagcga tagcggacct ggtgattaaa
     3181 aaaggtgcga tgaagtaaat tagtttgcgt ttaccttagg gtatggctac aatactaaca
     3241 gctgctaacg gttattactt gagagggagg aatgagtatg ggttcaacta tccttgtggt
     3301 tgatgattct gtttctatca gacaaagcgt tcgctttatt ttggaacaga gtggatacac
     3361 agttcacgaa gctgcagatg gtgcggaagg acttaaacgc cttggagagc aaaaggttga
     3421 tttggttata tcagatataa atatgcccaa catggatggt cttaccatga tcaagcagat
     3481 tcgggagacc gacaagatca agtttttgcc catattggta ctaactaccg aatcgcaagg
     3541 tactgtcatc gaagagggta agaaggctgg cgcgaccgga tggatcgtta aaccctttaa
     3601 tgatgttaaa ctcatcgaga cgatcaaaaa agtccttcct tgaagctaaa cccccagttc
     3661 gataaaccag aacaatacat cgggccgggg gattactgtg tgaccggcga ggatattgtt
     3721 atttccaccc tgttgggatc gtgtatcgcg gttgccttgt atgatctgga aaaagccatt
     3781 ggtggcctca atcatttcat gcttccattc acccgaaacc ttgatgcgca caagcctgaa
     3841 gatagtgccc gttatggtat aaattccatg gaaatgttga taaatgggtt gttaaagatg
     3901 ggagctcgtc gcgagaaatt acgttctaag gtttttggtg gcagttcagt actcagatat
     3961 caacaagaag ccacttataa tattccaaaa atgaatattg agtttatttt taattttttg
     4021 gatatagaga agataccgat agaatcctac tctgtgggag ggcctttccc tcgtaagata
     4081 tactattttc cccgaaccgc aaaggttttg atgcgctata gcaaacagga ttc
//
LOCUS       DAOQXQ010000057         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22198_length_4110_cov_3.238471, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000057 DAOQXQ010000000
VERSION     DAOQXQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_4110_cov_3.238471"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKH81_01585"
     CDS             <1..1128
                     /locus_tag="PKH81_01585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNQ96758.1"
                     /translation="DVLVSDDKGKLFTVFSSQGSKNELVLAENKAIDFLLGHSESIVL
                     KTQAVTNHLYTDIKPDLLKIFDLGKADALILLREGHRVVGIMLLGPKKRGSDYTEYDY
                     SVLTKLYSNFFLIMYYLKNIANESVVLTVDREIEFSGQVITSIQDNIDRINHEKVEAD
                     FITRSARKLGGDFIDFIKLSDEKYLFVMGDISGKGLNASMSMIILKSVLRTFLAETKD
                     FKQLAVKANLFIKNNLPKGTFFAGVFGLMDFTSNTLYYLNCGVPAMFLYTASYNNAIE
                     IQGDGKVLGFVKDIGKYLKVKKITLNPQDILLMTTDGLVDATNLRGERFGKDRVQRLL
                     MDNRSYPAGRMAQFICDNLGEFVSRELEDDITVLVIKYLSK"
     gene            1142..1468
                     /locus_tag="PKH81_01590"
     CDS             1142..1468
                     /locus_tag="PKH81_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ96759.1"
                     /translation="MESISIVEKNGPNYALLEVAGTINSYTYTEFQDKVYSLIKETNL
                     VLDLSRVTNLSSSGLGVIMAAFDDGETVGNKLYIMKPSEVVRLAIESTGFSEMFTVIY
                     SLTEVV"
     gene            1530..1892
                     /locus_tag="PKH81_01595"
     CDS             1530..1892
                     /locus_tag="PKH81_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96760.1"
                     /translation="MEAFILQCPLLTGENVSRAMILVTEYFDNIVMHNRFPKRLPVAI
                     SITRKKDIVISLAYYSCNFASLIRSDRDAIPYYDPVTRRYRGIGLRMCHALAKSIRYR
                     RGLLKKYIHIRLDVCDAK"
     gene            1877..2548
                     /locus_tag="PKH81_01600"
     CDS             1877..2548
                     /locus_tag="PKH81_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNQ96761.1"
                     /translation="MRREVIAFFDVDHTISRRATAVPFIMICIKRGYIRWWTLLAAPF
                     LFIAYRFFSVKMETLFKLSLPYLTGLSRATFDSIAEEAYHRFMEKSLYPGALREMEQL
                     RKEGTRVILATSSPFEAVYPSVKRCGIGAADVIATQFAYDAEGKFMGKLVGMPVFSRH
                     KCSIIQDFAERSGADLHYCSFYSDSIHDLPLLNSVGRPVAANPDLRLRFLARRRGWVI
                     KDFTK"
     gene            2545..3363
                     /locus_tag="PKH81_01605"
     CDS             2545..3363
                     /locus_tag="PKH81_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNQ96762.1"
                     /translation="MRTSYIALFLLVPLVSCTAQKKDDSSVSTAFQQPVFIAPSSIPR
                     PILDRLNADKDVFVPALEALIEQDTEGLLTLVDKKHLLASDMVPSDLVKLTKGKSYTL
                     SRDGFSLRSSAEASLERMATDARKEGVTILVSSTYRSWEYQKSLYARNVRELGQEVAD
                     RESAPPGASQHQTGTAVDFGTITDSYAQTRAGKWLSSNAWKYGWSLSYPDGYEQVTGY
                     KWECWHYRYIGEEAAAFQRQWFDDVQQYMLEFIHEWKLWRTSNLSVVDLPAVMS"
     gene            complement(3328..>4110)
                     /locus_tag="PKH81_01610"
     CDS             complement(3328..>4110)
                     /locus_tag="PKH81_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ96763.1"
                     /translation="LAITSIISQPSDKKHPWGHGRAETVGTVVLAFIILLAGSQLALS
                     ALAELRSGQVRPIPETIVLYVTASSVFGKLLLALSQWKLGKKANSAMILANAKNMTGD
                     IIISASVFLGIGAAHIFSLPIIDPIIALLVGIWIVKNGLSIFLEQNLELMDGNADDAL
                     YHSLFEAVKTVKGAGNPHRARIRKIASAWDIDLDIEVDGSKTVCQEQAIAEKVEKAVR
                     ERIPDVYDIMVHVEPAGAGQHPEQYGLCADDLTHHCRQINDT"
BASE COUNT         1039 a          916 c          986 g         1169 t
ORIGIN      
        1 gatgttttgg tttccgatga caaaggaaaa ctttttactg ttttctcgag tcaaggttca
       61 aagaatgaac ttgttcttgc agaaaacaag gcaatcgatt ttcttttggg gcacagtgaa
      121 tcaatcgtac tcaaaacgca ggcggtaacg aatcatttat atacggatat taaacctgac
      181 ttactcaaga tattcgatct tggcaaggct gatgcgttaa ttctgcttcg ggaaggccat
      241 cgcgttgttg gtattatgct ccttggtcca aaaaagcgcg gttcggatta taccgaatat
      301 gattactcgg tattaaccaa gctctattca aacttcttcc ttatcatgta ctacctgaaa
      361 aacatcgcaa acgaatcggt tgtgttaact gtagaccgtg agatagagtt ctctggccag
      421 gttatcactt ccatacagga caatattgat cgcatcaatc atgagaaggt agaagctgac
      481 tttattaccc gatccgcacg aaaacttggt ggcgacttta ttgactttat aaaattgagc
      541 gacgagaaat acttgtttgt aatgggagac atttccggta agggtcttaa cgccagtatg
      601 tcaatgatca tccttaaatc tgtgctgcgc accttccttg ccgaaaccaa ggactttaag
      661 caattggcgg ttaaggcgaa cctgtttatc aagaataatt taccaaaggg aaccttcttt
      721 gccggtgtgt ttggtttgat ggattttacc tccaacaccc tgtactacct gaactgcggt
      781 gtacctgcca tgtttttgta tactgcttcc tataacaacg caattgaaat tcagggcgac
      841 ggtaaggtct tgggctttgt taaggatatc ggaaaatatc taaaggttaa gaagataacc
      901 cttaatcctc aggacatctt gttaatgacc acagacggat tggtagatgc aacgaatctt
      961 cggggagagc gttttggcaa ggatcgtgtt cagcgcctgt taatggacaa tcgctcctat
     1021 ccggcaggac ggatggccca gtttatctgc gataaccttg gtgaatttgt ttcgcgcgaa
     1081 cttgaagatg atattacggt tcttgtaatc aaatacctga gcaaataagg ggtcggtaca
     1141 catggaaagt atttcgatag tcgagaaaaa cggaccgaat tacgcgctgc ttgaagtagc
     1201 gggcaccatt aattcgtata catacaccga gtttcaagac aaggtgtatt ctcttatcaa
     1261 ggaaacaaac ttggtgcttg atctttcccg ggtaaccaat ctttcgtcgt cgggcttggg
     1321 cgttattatg gcggcctttg acgatggtga aaccgttggc aacaaattat atatcatgaa
     1381 gccatccgag gttgtgcgcc ttgccattga gtcaactggt tttagcgaga tgtttaccgt
     1441 tatctattcg ctcaccgagg tcgtgtgatc ccgtggacgg tcggcattgt caaatattcc
     1501 tcgtccctga ctttgccagc cttccacaaa tggaagcgtt tatacttcaa tgccccttgc
     1561 ttaccggcga aaatgtttct cgcgccatga ttttggtcac agagtatttt gataacattg
     1621 tcatgcataa tcgatttccc aagcggcttc cggtagcgat ttccattacc cggaaaaaag
     1681 atattgtcat ttccctggcg tactattcat gtaactttgc cagtttaatc cgctctgatc
     1741 gggacgcgat tccttactat gatccggtaa cccgtcggta taggggtata ggactgagaa
     1801 tgtgccatgc cctggcaaag agtattcgct atcgcagggg cttgctcaag aaatacattc
     1861 acattaggct cgatgtatgc gacgcgaagt aattgctttt tttgatgttg atcatactat
     1921 ttcccggcgc gcgacagctg ttccctttat catgatctgc attaagcgcg ggtatattcg
     1981 ctggtggacc ttgcttgccg ctccgttttt gttcatagcg taccgttttt tcagcgtcaa
     2041 gatggaaaca ctctttaagt taagcttgcc gtatttaacc gggctttccc gggctacctt
     2101 tgatagcata gcagaagagg cgtatcatcg ctttatggaa aagagtttgt atccaggagc
     2161 cttacgggag atggaacagc ttagaaaaga aggcacccgg gttattctgg ctacgtcctc
     2221 accctttgag gctgtatatc cctcggttaa acgatgtggt attggagctg cggacgttat
     2281 tgcaacgcaa ttcgcatacg atgcagaggg aaaatttatg ggcaagctgg tcggtatgcc
     2341 cgttttttcc cgacacaagt gttcgataat acaggacttc gctgagcgca gcggagccga
     2401 tttacactac tgttcgtttt attcggacag catacacgac cttccgcttc ttaattcggt
     2461 tgggcggcct gtcgccgcaa atcctgatct tcgcttgcga tttttagcac ggaggagggg
     2521 gtgggtaata aaggatttta caaaatgaga acatcgtaca tcgcactctt tcttctcgtt
     2581 ccgcttgtat catgtacagc gcaaaagaaa gatgattctt ctgtaagcac tgcttttcaa
     2641 cagccggttt ttatagctcc ctcgtctatt cctcgcccta ttcttgatcg gctcaatgcc
     2701 gacaaggatg tgtttgttcc ggctctggaa gcccttattg aacaagatac cgaaggctta
     2761 ttaacccttg tcgataaaaa acacctgctt gcttcagata tggttccttc tgatttggta
     2821 aaactgacaa agggaaaatc ctacaccctg agccgtgacg gtttttctct tcgctcttcg
     2881 gcagaggcat cgcttgagcg aatggccact gatgcgcgta aggaaggggt tactattctg
     2941 gtcagttcta cttaccgctc ctgggaatac caaaagagtt tatatgcacg aaacgtgcgg
     3001 gagttgggac aggaagtggc cgaccgggag tctgcgcctc ctggagcgag tcaacatcaa
     3061 accggtacag ccgttgattt tggtacaatt accgactcat atgcccaaac gcgcgcgggt
     3121 aagtggcttt cttccaacgc atggaaatat ggctggtcct tatcgtatcc tgatgggtat
     3181 gagcaggtta ccggttataa atgggaatgt tggcattacc gatatattgg ggaagaagcc
     3241 gcagcgtttc aaagacaatg gtttgatgat gttcaacagt acatgctaga gtttatccat
     3301 gagtggaaac tgtggcgaac atcgaatcta agtgtcgttg atctgcctgc agtgatgagt
     3361 taggtcgtcg gcgcaaaggc cgtattgttc aggatgctgg cctgctcccg caggttcaac
     3421 gtgtaccata atatcgtata cgtcggggat gcgctcccga accgcctttt ctactttttc
     3481 cgctattgct tgttcttggc ataccgtctt tgaaccgtca acctcaatgt ccaggtcaat
     3541 atcccatgca gaggctatct ttcgaatacg agcccggtgt gggttccctg cgcccttgac
     3601 tgtttttact gcttcaaaaa gggagtggta taaggcatcg tccgcattac cgtccatcaa
     3661 ttcaaggttc tgttcaagaa agatagaaag gccgtttttt acaatccaga ttcctaccag
     3721 aagggcgatt atcggatcta taatcggcaa ggaaaaaatg tgtgcggctc caatgcccaa
     3781 aaagaccgag gccgagatga ttatgtctcc ggtcatgttt ttagcatttg ccaaaatcat
     3841 tgcgctgttc gcttttttcc ccagcttcca ttgagacagg gcaagcaaaa gcttgccaaa
     3901 aacggaagag gcggtaacgt agagcacgat cgtttcggga atcggacgca cttgtcccga
     3961 gcgtaactcc gctaaagcgg aaagggcgag ttgagatccc gcaagaagaa taataaaggc
     4021 aagcacaaca gtgccaacgg tttccgccct gccgtgaccc caaggatgct ttttatcgga
     4081 aggttgacta attatgctcg tgatggccag
//
LOCUS       DAOQXQ010000058         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22279_length_4100_cov_2.819283, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000058 DAOQXQ010000000
VERSION     DAOQXQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22279_length_4100_cov_2.819283"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PKH81_01615"
     CDS             <1..167
                     /locus_tag="PKH81_01615"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ96764.1"
                     /translation="IPQHTIDTKGVERTIAISGRHDVCACPRVVPVIEAMTAITLMDL
                     LLRNRASRIY"
     gene            204..563
                     /locus_tag="PKH81_01620"
     CDS             204..563
                     /locus_tag="PKH81_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96765.1"
                     /translation="MTITDVLSAIEAEAIADTWRDDVEINSACGADLMSDVMAFVKDQ
                     VLLMTGLINVQVIRTAILMDIQAVCFVRGKSPNEEMIEMAKANGIVLLKTKLPLYISC
                     GKLYTAGVREGGVRKID"
     gene            576..998
                     /locus_tag="PKH81_01625"
     CDS             576..998
                     /locus_tag="PKH81_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ96766.1"
                     /translation="MEFHYEVSGNDFARAGLASSEIKKTLQRLGLPHEVIKRVAVAMY
                     EAEINMVVHAGGGVADVRIDKELITIVMCDKGPGIEDLSKAMEDGYSTASEQVRELGF
                     GAGMGMSNMKKNADEMYIDTAKGKGTCVTLKIRIPEHS"
     gene            995..2302
                     /locus_tag="PKH81_01630"
     CDS             995..2302
                     /locus_tag="PKH81_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNQ96767.1"
                     /translation="MSDRRYHSVILDYDACKGCTICVTGCPAEAIRVRDGKAQIIAER
                     CIDCGECIRRCPHRAKKAQCDDFSLFHSDSNLLQIALPAPSLYGQFPDQYSIADIHAA
                     LIELGFDAVFPVAAVTDSIARAGCAFLDRAMHPDIPRPLISSSCPTIVNLVQIRFPSL
                     LDHILPVLAPMELAGRVAKQLLDGIAGKPIKAWFISPCPGKITEAIAPLGGEKTSVDG
                     VFSMHDVYLPLLQALKKGNSGYTGILPAAASEIAWGRSGGEAEATFPGGKDSYLAIDG
                     MDQCIKILEAVEDGQLTDIDYLELMACPLGCVGGAITAINPSIAAHTLKRRERLFTKT
                     VDLPPECVCEQDACLRSAEFPPRPALLLDKDFHRAMSMMEQMEEIHKALPGLDCGCCG
                     SPNCHALAEDIVRGMAKKTDCVIILKEQYRELLDSKQKDGHEG"
     gene            2292..2912
                     /locus_tag="PKH81_01635"
     CDS             2292..2912
                     /locus_tag="PKH81_01635"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNQ96768.1"
                     /translation="MKDKALFRKERKALLLGQGPASVHPNPTLLCFVHQYRTIFAYQA
                     LGYELSPALLLEEAHTKGILIALPRIENRSLIFRKVDNLCHSCETGSFSIREPKLSCA
                     EVYPDGDFPLPLLIMIPGLAFTRQGGRLGRGGGYYDRFLCDLRKRESLSGQVTLAGIC
                     LSSQIVNALPEEAHDERVDCLIDENGSIFYVDSKKGVTDGRDQIGN"
     gene            2887..3480
                     /locus_tag="PKH81_01640"
     CDS             2887..3480
                     /locus_tag="PKH81_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96769.1"
                     /translation="MGEIRSAIDIALEKTAHIDSDGKSSAQREIKNRGKRAGSAFLDT
                     ADGELLQKAMQAANKEEGLLLIEGALSILMAALRLPAQEEDLNKVARIGEALQIIIPQ
                     GEMKELFDQLQQILHQFLGEKSQLKQALEQQFMPKLRAKQQELSRRYGQNIPLEPTQD
                     PEFMATLNKNMWMLEGKYDGVLEEVRARIREAGGISE"
     gene            3559..3631
                     /locus_tag="PKH81_01645"
     tRNA            3559..3631
                     /locus_tag="PKH81_01645"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3592..3594,aa:Asn,seq:gtt)
     gene            complement(3763..>4100)
                     /locus_tag="PKH81_01650"
     CDS             complement(3763..>4100)
                     /locus_tag="PKH81_01650"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ96770.1"
                     /translation="LVSGGTDNHLMLIDLTNKGVTGKEMAVALEKAGIVLNYNTVPGE
                     TRKASDPSGIRLGTPSVTSRGFGEAEMKKIAAWMDAVSTHIADEAFLAATAKDVEALC
                     SRFPVPGID"
BASE COUNT         1047 a         1011 c         1076 g          966 t
ORIGIN      
        1 gcatccctca gcacaccatt gatacgaagg gagtagagcg aaccatagcg atctctggcc
       61 gccatgatgt atgcgcctgc ccgcgggttg ttccggttat cgaagcgatg acggctatta
      121 ccctgatgga tctgttgttg cgaaacagag cctcccgcat ctattgactt tacgccgggg
      181 ttgaaccatg gtatcctttt tttatgacaa tcactgatgt actttctgcc attgaggcag
      241 aagcgatagc cgacacctgg cgcgatgatg tcgaaatcaa ttcagcttgc ggagcagact
      301 tgatgagcga tgtgatggcc tttgttaagg accaggtgtt attgatgacc ggtttgatca
      361 atgtgcaggt tattcgcacg gcgattttaa tggatatcca ggctgtttgt tttgttcggg
      421 gaaaaagccc gaacgaggaa atgatcgaaa tggccaaggc aaacggtatt gttctgctca
      481 aaacaaaact tccgctatac atatcttgtg gcaagctcta taccgcgggt gtccgcgagg
      541 gcggcgtcag gaagattgac tagcgcgggg cgggaatgga gtttcattac gaggtatccg
      601 gcaatgattt tgccagagca ggactcgctt catcagaaat aaaaaaaacg ctccagcgac
      661 ttggcttgcc ccatgaggta atcaagcgag ttgcagttgc catgtacgaa gccgagatca
      721 acatggtggt acatgctggt ggcggagttg ccgacgtacg cattgataaa gaactcatta
      781 cgattgttat gtgcgacaag ggcccgggca ttgaggatct gagcaaagcg atggaagacg
      841 gctattccac agcaagcgaa caggtgaggg aacttggctt tggcgcgggc atgggtatga
      901 gcaatatgaa gaaaaacgca gacgagatgt acatagacac cgcaaaagga aagggcacct
      961 gcgtcaccct taaaatccgc atcccggagc attcatgagc gatcgtcgct atcattcggt
     1021 tattcttgac tatgacgcat gtaagggctg taccatctgt gtaaccggtt gccctgcgga
     1081 ggctatacgc gttcgtgacg gaaaggcaca gattattgcg gaacgatgta ttgattgcgg
     1141 tgagtgtatc aggcgttgcc cgcatcgggc aaaaaaagcc caatgcgacg atttttcgct
     1201 cttccactct gacagtaatc ttctacagat tgcgctaccg gccccttccc tctatggtca
     1261 atttcccgat caatacagca ttgccgatat ccacgccgcc ctgattgaac tcggctttga
     1321 cgccgttttc ccggttgctg cggttaccga ctccatcgca cgcgccggat gcgcattttt
     1381 ggaccgtgca atgcatcccg acattcccag accgcttatt tcctcaagct gcccgacaat
     1441 agtaaatctt gtgcaaatac gttttccgag tcttctcgat cacatcttgc ccgttcttgc
     1501 ccccatggaa cttgcaggtc gggtagcaaa gcagttactt gatggtatag ccgggaaacc
     1561 gataaaggcc tggtttatct ctccgtgccc gggaaaaatc accgaggcga ttgcaccatt
     1621 gggaggagaa aaaacctcgg tagacggcgt tttttctatg catgatgtat atctacccct
     1681 gcttcaagcc ctaaaaaagg gtaatagcgg ctacacagga attctgccgg ctgcagcttc
     1741 agagattgcc tggggtcgct cagggggaga ggcagaagca acctttccgg ggggcaagga
     1801 ttcctatctg gccatagacg gcatggacca gtgtatcaag atattggaag cggtagagga
     1861 cggacaactt accgatatag actatctgga actgatggca tgcccactcg gatgtgttgg
     1921 cggggcaatt accgcaatca atccctcaat cgctgcccac accctgaaac gacgagaacg
     1981 actctttacc aaaacagtag accttccccc tgaatgcgtc tgtgaacaag atgcatgcct
     2041 gcgatcagct gagtttcctc cccgccctgc cctgcttctt gacaaggact ttcatagagc
     2101 tatgtccatg atggaacaaa tggaagagat tcacaaagcg cttcccggcc ttgactgcgg
     2161 ctgttgcggt tcacccaatt gccatgccct ggcagaagac atagtacgcg gtatggctaa
     2221 aaaaacagat tgcgtcatta tccttaaaga acaatatcgc gaacttctgg actcaaaaca
     2281 gaaggacggg catgaaggat aaagcccttt ttcgtaagga gcgtaaggcg cttctcttgg
     2341 gccaaggtcc tgcttcggtg catccgaacc cgacactgct ctgctttgta caccagtatc
     2401 gcaccatctt tgcctatcag gcacttggat acgagctttc ccctgcctta ctcctggaag
     2461 aagcacatac aaagggaatc ctcatcgccc tcccgcggat cgagaatcga agcttgatat
     2521 tcaggaaggt agacaacctt tgtcactcat gtgagactgg ctctttttcc atacgtgagc
     2581 caaaactatc ctgtgccgag gtctaccctg atggggattt cccccttccc ctgcttatta
     2641 tgatacctgg cctggccttt acccgacagg gaggcaggct gggccgcggg ggtggctatt
     2701 acgaccggtt tctttgtgac ctgcgcaaac gtgaatctct tagcgggcag gtaacccttg
     2761 ccggaatctg cctgagttca caaattgtga atgcactacc cgaagaggca catgatgagc
     2821 gagttgattg cctaatcgat gaaaacggaa gtatatttta tgtagattcg aaaaaaggag
     2881 ttactgatgg gagagatcag atcggcaatt gatatagccc ttgagaaaac cgcacatata
     2941 gatagcgacg gaaagagctc tgcgcaacgg gagataaaaa accggggcaa gcgggcggga
     3001 agcgcatttt tggatactgc tgatggggaa ctgttacaga aagcgatgca ggctgcgaat
     3061 aaggaagagg ggctgctcct gattgaaggt gccctttcca tattgatggc agccctgcgg
     3121 cttcccgcgc aagaggaaga cctgaacaag gtagctcgaa taggagaagc actccagata
     3181 attataccgc aaggggaaat gaaggaactt tttgaccagc ttcaacaaat actccatcag
     3241 ttccttggtg agaaatcaca gctcaaacag gcccttgagc agcaatttat gccaaagctc
     3301 cgggcaaaac agcaggagct ttcccgtcga tatggacaga acattcccct tgaacccacc
     3361 caggatcccg agtttatggc gacattaaac aagaatatgt ggatgctgga agggaaatac
     3421 gatggcgtgc ttgaggaagt tcgcgcccgg attcgagaag ccggcggcat cagcgaataa
     3481 aaaaggatac agggcgtcgc ttgacttttt tttgttttta ggataattta accctcgcct
     3541 gccacacggc aggcgctttc cgcggtagct cagtcggcag agcaagtggc tgttaaccac
     3601 tgggtccggt gttcgaatca ccgccgcgga gtaacaaaac ccatccgaaa ggatgggttt
     3661 ttcttttttt tgatcatgta tgagtgcttg aaggattgtt ggtaaaaaaa cgccgcccgg
     3721 gaaggacggc gtttttcact tcatctataa aaaccagggt aatcaatcaa taccaggaac
     3781 cgggaagcga gagcacagag cctctacgtc ctttgcggtt gccgcaagga aggcttcatc
     3841 ggcgatatgg gtactgaccg catccatcca ggcggcaatc tttttcatct cggcttctcc
     3901 aaaaccacgg ctcgttaccg agggggtgcc aagacggata ccggaaggat ctgatgcctt
     3961 gcgtgtttcc ccgggcacgg tgttgtaatt aagaacaatc cccgccttct ccagggcaac
     4021 tgccatttct tttcccgtaa cgcccttgtt cgtcaaatca atgagcataa ggtgattatc
     4081 ggtaccgcct gaaacgaggg
//
LOCUS       DAOQXQ010000059         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22281_length_4100_cov_2.696663, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000059 DAOQXQ010000000
VERSION     DAOQXQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22281_length_4100_cov_2.696663"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1196)
                     /locus_tag="PKH81_01655"
     CDS             complement(243..1196)
                     /locus_tag="PKH81_01655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ96771.1"
                     /translation="MITFNQETIIKETLDSILTQDHSPLEVIVCDDASTDNTGVKCCK
                     WIEKNRKKFYRVLYHRNEKNLGIMGNELTGIGLAQGVFLKVLAGDDLLAPGAIKKAAE
                     YFKNNPNALVVFGNIKSFYEKKDPISFIYTEPRTTAKCFFRLSATEQFRILSNGNPVP
                     APGAFYRNSFFERVSLDSLQVKHIEDWAVWLSATSQHISLNYFDFDAIYYRLCSPTKE
                     KYDPDFHKECLMDCLSLIENIIKPNKRLLSIIELLAVWANEKRLNLLLKNRNLSGSIF
                     QGLLSLFRPFIRYKLYLGAMAEMMGSETEKIDKNHNHGSFR"
     gene            complement(1596..2606)
                     /locus_tag="PKH81_01660"
     CDS             complement(1596..2606)
                     /locus_tag="PKH81_01660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ96772.1"
                     /translation="MTDLDPLVSVVVPVFNMESVIGTTLNDIIQQKDIIPEVIVVNDA
                     STDGSLGIISEALRKYRGPHKIISIDKNLGASYARNIGLDASVGKYVFFIDGDDRMAE
                     ETLVKMLNLAETHQADIVFSGFRVCGPGLQDGKVYSWKGSKEGFCNPREVLRSFLRGK
                     RFLNASNTLYSKEFLEYHGIRFPRGCRFAEDREFIVKALFHAKRAAFTAEPLVSYIQH
                     PGQTTNKPGTGINKYAHSVGVCLRLKKYFENRESGESILNLLNTYELPDALLKMLTET
                     AKANDLAKFQKVINSQFIRSALRPGFRSILFKPEVAYKAWEALNLPRALFRSYKKKGR
                     GH"
     gene            3030..4049
                     /locus_tag="PKH81_01665"
     CDS             3030..4049
                     /locus_tag="PKH81_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNQ96773.1"
                     /translation="MKRFAALVAVLALVAFAAPAFAANPFMDVPMNHWAYDAVSQLAS
                     RGVVSGYPDGAFKGEWKATRYEMASVVARALAYVDMNKASKQDLELLKKLVVEFKDEL
                     DALGVKVDELDARVAVLEKDIGGWRIWGEFRFDAKFSDGNKFVYNSVGGGSGTSWEHV
                     TPISTNIWTKGEKDFEISRYRIWLSKRIDDKVTFIARLGGADVAFERYYLDVKLPWDA
                     NLLVGKWLFDWEGDDNLYNDNDAIFTDRAVQGFYLKKTFGMGDFHLFASQGDWTYNTG
                     YSTDWDWFYDGTGNPGNVANWDYRRTGWNTDTGGGEAMLYGARVNVAFNENFGGSLNF
                     LYLDG"
BASE COUNT         1037 a          940 c         1028 g         1095 t
ORIGIN      
        1 gattgggatt cttgataggg cttcatcttt tcaccttatt gctatgtgct tttccgtcat
       61 ccttaagaat tacctagctt tcaagaaaag attacactcc aacgtttcct tctgactgat
      121 gtagcatgtg gttttcttga ttctaccaac gacaatacac cgctattttt caactccaaa
      181 catcgaattt ggcttaacac attttgaaaa acgaaaataa tcttttatca atgagacttg
      241 ttttacctga aggagccgtg gttatgattc ttgtcaatct tttctgtttc tgaacccatc
      301 atttcagcca ttgcgcccaa atagagctta tatcgaatga agggccggaa taggcttagt
      361 aaaccctgaa aaatagagcc agagagattt ctgtttttca aaagcaggtt taaccttttt
      421 tcgttagccc aaacggccaa tagttcaatg atgctaagaa ggcgcttgtt cggtttgata
      481 atattttcga tcagggaaag gcaatccatc aggcattctt tgtggaaatc tggatcgtat
      541 ttctctttcg ttgggctaca taacctgtaa tatatcgcat cgaaatcaaa ataattaagg
      601 ctgatatgtt ggcttgtggc ggaaagccat acagcccagt cctcaatatg ttttacttgt
      661 aaagaatcaa gagatactct ttcaaagaag ctgtttctat aaaaggcacc gggcgctggt
      721 accgggttcc cgtttgaaag gatcctgaac tgttctgtgg cggaaagacg gaagaagcat
      781 tttgctgttg ttcttggttc tgtgtaaata aaactaattg gatctttttt ttcataaaaa
      841 gacttgatgt ttccgaagac aactagcgcg tttggattat ttttaaaata ttccgccgcc
      901 ttttttattg ctcctggggc aagcaagtca tcgccggcga ggacttttag aaaaacccct
      961 tgcgcgagcc cgatgccggt cagttcgttt cccatgatgc caaggttttt ttcgttcctg
     1021 tggtacaaaa cacggtagaa tttctttctg tttttctcga tccacttaca acatttaacc
     1081 ccagtgttgt cagtggacgc gtcgtcgcaa acgatcactt ccagtgggga gtgatcctgc
     1141 gtaaggatcg agtccagtgt ttctttaatg atcgtttcct ggttaaaggt gatcacaatt
     1201 atcgaaaata ggcgctggtc cattgttatt ctcccttaga aaaatgtttg tgttgtaatt
     1261 caaaagtgct ggaatgattg gtttaacaac aatccagttg aatacgattg atttgcacaa
     1321 acggttttgt tttaaagaaa cattgttaga aaagctgtgt gaatgccaaa tcacacggta
     1381 attggctcag ttctcttttt tatctcgcaa gagtttcttt cgtgctaacc aaccattaac
     1441 ggtaggtttt cgcttgcaat ttattcttaa tcattacatc gctatcttga acgaaggaaa
     1501 ggaggcactt ttcttaccta aggcttacaa ccattgagga taatacagac cttcaaagaa
     1561 atcaccaagg accaaaaatt cctggccgtg cgactttagt gtcctcttcc ttttttcttg
     1621 tacgaacgga acagggctct cggtaaattg agagcctccc aggccttgta agccacttcg
     1681 ggtttaaaaa gaatggacct gaagcctgga cgcagggcag atcggatgaa ttgagagttg
     1741 attaccttct gaaattttgc aaggtcattc gctttcgctg tttcggtaag cattttcagc
     1801 aaggcatcgg gcagttcgta agtgtttaat aggtttaaaa tgctttcccc gctttccctg
     1861 ttttcgaagt atttttttag tctgaggcag acccccacgg aatgggcgta tttgttgata
     1921 cccgttccgg gcttgttcgt ggtctgtccc gggtgctgga tgtaactcac gaggggttcg
     1981 gccgtgaagg ccgctctttt cgcatggaaa agtgctttga caataaattc cctgtcctcc
     2041 gcgaaccggc acccgcgggg gaaccggatt ccgtgatatt ccaggaattc tttgctgtaa
     2101 agagtgttgg aggcatttaa aaagcgtttc cccctcaaga aactgcgcag cacttcccgg
     2161 gggttgcaaa aaccttcttt cgaccctttc cacgaataga ccttcccgtc ctggagacca
     2221 ggtccgcaaa cacgaaagcc ggagaagaca atgtcggcct ggtgggtttc ggccagattc
     2281 agcatcttaa caagggtctc ttctgccatc cggtcatcgc catcgatgaa gaaaacgtat
     2341 ttacccactg aagcatcgag gccgatgttc ctggcatagg acgcacccaa gtttttatca
     2401 atggaaatga tcttgtgggg accacggtat ttcctgaggg cttcgctgat gattccgagg
     2461 ctgccgtcgg tagaggcatc gttcacgaca atgacctcgg ggatgatatc cttttgttgt
     2521 ataatgtcgt tcaaggtggt tcctatcacg gattccatgt tgaataccgg cacgacgact
     2581 gaaaccaggg gatccaggtc cgtcaccttc cttacaaggg tgaattgagt gctacaatag
     2641 gataacagga aagcgcaaaa aagagttcgt ctgttaacag gacccgggaa gaagggaaag
     2701 cctggtagca ctatctcgga gacatgttta tcacggtaaa tgggactaaa gtcctattcc
     2761 ccgtaggtag ttttacgggt aaaattccac cgggtatacc gagagcacct tgaaaacaga
     2821 accttggaca cgcttcaaat tccgcggtga cccctgacgg caggaaagga ggtgcaaccc
     2881 ccgaggccaa aatgggagag cggatagtag aagccggacc tttcgggaaa gggttcgcac
     2941 gggaggcgga aggaaacttg gatacttccc actgtatcgt gcgtgcaccc cggagggccg
     3001 gaatacataa cccaaggggg ttaaagagaa tgaaaagatt cgcggcacta gtagcagtac
     3061 tcgcgctcgt cgcgtttgcc gccccggcat tcgcggccaa cccgttcatg gatgtcccca
     3121 tgaaccactg ggcctatgac gcggtgtccc agctcgcttc caggggcgtg gtgtcgggtt
     3181 atcccgatgg cgccttcaag ggcgagtgga aggccacccg ttacgagatg gcctccgttg
     3241 tggccagagc ccttgcctac gtcgacatga acaaggcctc caagcaggac ctggagctcc
     3301 tcaagaaact cgttgtggag ttcaaggatg agctggatgc cctcggcgta aaggttgacg
     3361 agctcgacgc cagggtagct gtcctggaga aggatatcgg cggctggagg atctggggcg
     3421 agttccggtt cgacgctaag ttctccgacg ggaacaagtt tgtatacaat agtgtcggag
     3481 gtgggagtgg gactagttgg gaacatgtca ccccaattag cacaaatatc tggacaaagg
     3541 gcgagaagga cttcgaaatc agccgttatc gaatctggct gagcaagaga atcgacgaca
     3601 aggttacctt cattgcccgc cttggcggag ccgatgtcgc ctttgaacgt tactacctgg
     3661 atgtcaaact tccctgggac gcaaacctgt tggtgggtaa gtggctcttc gactgggaag
     3721 gcgacgacaa tctctacaac gacaacgacg ccatattcac agacagggca gtacagggct
     3781 tctacctgaa aaagaccttc ggcatgggcg acttccatct cttcgcatct cagggagact
     3841 ggacctacaa taccggatac agtacagatt gggattggtt ctatgacgga actggcaacc
     3901 caggtaatgt agctaactgg gactatcgcc ggaccggttg gaacaccgat acaggcggcg
     3961 gcgaagccat gctctatggc gcgagggtta acgtggcctt caacgagaac tttggaggca
     4021 gcctcaactt cctctatctc gacggctagg gcggttggga ccagaaggta tggtgggtag
     4081 ccccctacgt taacttcact
//
LOCUS       DAOQXQ010000060         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22505_length_4072_cov_3.558875, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000060 DAOQXQ010000000
VERSION     DAOQXQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22505_length_4072_cov_3.558875"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1456
                     /locus_tag="PKH81_01670"
     CDS             215..1456
                     /locus_tag="PKH81_01670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ96774.1"
                     /translation="MRILWLSSLSLISHDEGGGSWQRAIAPHLLRSGRVTLGNVTVGP
                     VSQTVREDYGGISQWIVPGVNNSNKNYLPSEAAIRDIVRVAEEFRPDIVHVWGTEFFW
                     GLLTGRRILSYPSLLEIQGLKGPLSRVFTGGLSLKEILACIGIKEILRRHTILQGKKR
                     FARWAYFENEIIAKHSNISTQSPWAEAWIRFINPSCNLFHTELVLRDAFYESEPWKPS
                     EDFNIFTSAVAPAPFKGLHDAVRAVAILRDRIPSIKLRIAGGHQRKGIRQEGYIRWVN
                     KMIRRYDLSKNVDWLGSLSEKEVAQELVGCAVALVPSYCETYCVAFAEAMQLGVPVVS
                     SFTGGAAWLAKDEETALFYPAGDHVMCAHQLWRILTNPELARCLSGKGREIADGRNDP
                     EKIVLNQIDIYRRVLHANKCT"
     gene            2631..3761
                     /locus_tag="PKH81_01675"
     CDS             2631..3761
                     /locus_tag="PKH81_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ96775.1"
                     /translation="MAGTKRIQNAINSLKQYPDIAIRVILQRQSSEHNALSGVHEGTP
                     YKTVMGNLFRSKMFILLPVLYYKSIVALKGALRPNKKNIIYFYGPLFLDSIVPLNYAK
                     RIGYKIVFDVIEDYDLSNEVSGSFYHHIRSNAANWFSSRIIDLSSGIIVISSHLEKKY
                     EALTKGEVPIHYMPISVNMDRFPEKPRQSKSTISLLYAGSFSQKDGVHVLLDAFDRLA
                     NRFGNVRLILTGSGERKAIKEFFARVEASPYKNRIEFKGYLNDADYYAMLNDVDIPCM
                     SRVDLAFAHAGFPFKLGEFLATGKPVIASRVSDIDRFLVHGHNAMVVQPGLSEEICEA
                     VEFLINNPESAEAIGMKGREVAKLSFDFKQQGKALLAFLQNL"
     gene            3891..>4072
                     /locus_tag="PKH81_01680"
     CDS             3891..>4072
                     /locus_tag="PKH81_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96776.1"
                     /translation="MYQADGIFLAHRRLAILDLSERGKQPMSYAEGRYWITFNGELYN
                     FVEIKNTTVGRETKIA"
BASE COUNT         1108 a          826 c         1036 g         1102 t
ORIGIN      
        1 tttttgatcg gtataatacg cggcggcccc atcagggtct caacgacagg atcccggaag
       61 atgtgtactg gagcacgctg ccgaaagtaa aggatgcggt atgaacagaa acggcgaacc
      121 atttaaaatc ttctggaggc tgtctgaaaa ggcttgaccg tctctctgga atctttaaga
      181 gatacgcact tcggtgaatc aaagcaacgt cattatgaga atcctctggt tgagctcatt
      241 atcgttgata agccatgatg aaggtggcgg cagttggcag cgtgccatag cgccgcatct
      301 tctgcgaagt ggccgggtaa ccttgggaaa tgtaactgtt ggtccggtat cccagacagt
      361 gcgagaggac tacggaggga ttagccagtg gattgtaccc ggtgtcaaca attctaacaa
      421 gaactattta ccatcagaag ctgcgattag ggatattgta cgagtggcag aagagtttcg
      481 acctgatatt gtgcatgtct ggggtacaga attcttttgg ggcttgctta caggaaggag
      541 aattctctcc tacccatcgc tcttggagat tcaggggttg aaaggacctt tatcacgtgt
      601 atttaccggt ggtctttctc ttaaagaaat tcttgcgtgt atcggtatca aggagattct
      661 gcgaagacat accattctgc aaggaaagaa aagatttgcc agatgggctt actttgaaaa
      721 tgaaatcatc gccaaacact cgaacataag cacacaatca ccgtgggctg aagcctggat
      781 caggttcatt aatccgtcct gcaatctgtt ccataccgag cttgtcctga gggatgcctt
      841 ttatgaatca gaaccgtgga aaccgtccga ggactttaac atttttacct cggctgttgc
      901 tccagcacct tttaaggggt tgcatgatgc agttcgcgcg gttgccattc ttcgtgatcg
      961 gatcccttca ataaaactta gaattgctgg ggggcatcag cggaagggga ttcgccagga
     1021 aggatacatt cggtgggtta acaagatgat caggcgatat gatctttcga aaaatgtgga
     1081 ttggcttggc tccttgtctg aaaaagaagt agcccaagaa ctggtgggtt gtgccgtggc
     1141 gctcgtgcca agttactgtg aaacctattg cgtagccttt gcggaggcaa tgcagcttgg
     1201 tgtaccagtc gtctcttcgt ttacgggagg tgcggcatgg cttgcgaagg atgaagagac
     1261 ggccctcttt tatccagctg gagaccacgt gatgtgcgct catcaactct ggagaattct
     1321 tacgaatccg gaacttgcga ggtgtctgtc agggaaaggg agagaaattg cagatggtcg
     1381 aaatgatccg gagaaaattg tattaaacca gatagatatt tacaggcgag tactacatgc
     1441 gaataagtgc acctaacaat ttgtcaaggt tttgtatctc tttcctcttg agagaggtaa
     1501 ggtatcacgc ggtatggtac aggttggtcg ttatggcctc cctatatgat ggaatgcttt
     1561 tgtgacattg ttcggagcaa cagttgggaa gaattttgga tattgcttgt gtttgccggt
     1621 gaggtgtgga tctcaaccgc gggggccgtt agacaccgcg atccctgata caaccttcaa
     1681 gaaatacctg cattacacga ttggattttc caggatccac ttgatatgac aaagtattct
     1741 tagctgtttc tcgtaggatg caaggcttcc aaggatttaa attctcattg aattcaattt
     1801 ccattcttga tggaggatat catgcgtaag atagaccgcg atagcctacc acatttagtc
     1861 aacgttgctt gtagaatcat tagcaggcat tactttacac tactggcgaa aatagctgcg
     1921 tggtggtggg gtgtggacct cgctggaaat tgtacttttt atgggcgcgt gagatttttt
     1981 cgacatcctc gaagcagaat tgcgattggg agcggttgtc aatttagatc ttctcctaca
     2041 tcaaatccga tgggaaataa tagaccttgt attctaacta ctttggacga cggtgccgaa
     2101 atcgtaattg gaccgaattg tgggtttagc ggaacgagta ttgtatgtaa agaaaaaata
     2161 actttaggca agaatgtgcg atgcggtccc aatacgacaa ttatggatac agacgggcat
     2221 gatgatgatc ataggtctgg aaccaataaa ccagtcttga tagaagatgg tgtctggttg
     2281 ggagctaacg tcagcgtata tcggggtgtt atgattggcg agaacacatt tattgccgct
     2341 tctagtacag ttacacgatc ttttcccggc aatacattta tagcaggctc gccagccaga
     2401 ccgtacaaga aaatggaata ggcagcgaac cgtgttaaag gatgtagcta cttatgcaaa
     2461 cacatttctt tccgcttggg gactttcgaa ttattctctg gctgccacag caattgttgt
     2521 tagtatccca gccaacccat agaaaagatt gacaaatttc aactaaaggc caaataatca
     2581 gaaaaaaaat caatcttgta tttgcgggcg gtttcactta tcccaggggt atggcaggaa
     2641 caaagcgaat ccagaatgct atcaactcac tgaaacaata ccctgatatc gcgatacgag
     2701 tcatacttca gcgccaatca tccgaacaca acgcgctctc gggagtgcat gagggcacac
     2761 cctacaagac tgtcatgggg aacctatttc gaagcaagat gtttatctta ctgccagtcc
     2821 tttattataa gagcatcgtt gcattgaaag gggcattacg gcctaataaa aagaatatta
     2881 tctattttta tggccctttg ttccttgaca gtattgtacc cctgaattat gcaaaaagga
     2941 taggttacaa gatcgttttt gatgttatcg aggattatga tctgtcgaat gaggtttctg
     3001 gttcgttcta ccaccatatt aggagcaacg cggcaaactg gttttcttca aggatcatag
     3061 acctgtcttc gggaatcatc gttattagct cgcaccttga aaagaagtat gaggctctta
     3121 ccaagggaga ggtgcctatt cactatatgc cgatatctgt caatatggat cgttttcctg
     3181 aaaaaccaag gcaatctaaa tcaaccatat ctctcttata tgcaggtagt ttcagccaaa
     3241 aggatggagt gcacgtgctt ctcgatgcat tcgatagatt ggcgaacaga tttggaaatg
     3301 ttcgtttgat tctcactggc agcggtgaaa gaaaggcaat caaagaattt tttgcccgtg
     3361 tggaggcatc gccttataag aatcggattg aattcaaagg ttatctgaac gatgcagact
     3421 attatgccat gctcaacgac gttgacatac cctgtatgtc ccgtgtggac cttgcttttg
     3481 cacacgctgg cttcccgttc aagctaggtg agtttcttgc aaccggtaag cccgtcatag
     3541 cctcgcgggt gtccgatatc gataggttcc ttgtccacgg acataatgcg atggttgtgc
     3601 agcccggttt gagcgaggaa atatgtgagg cagtggagtt cctgattaat aatccggaat
     3661 ctgcagaggc catagggatg aaaggacgtg aagtggccaa attgtctttc gattttaagc
     3721 agcagggaaa agcgttgctt gcctttctac aaaatctata agcagataaa tgaaggcgga
     3781 gcagacaaca agctatgtgt ggaatctttg gatataaagg gaaaatagac caacaagttg
     3841 ggctaaagtg tgtcaggaaa ttagctcatc gtggcccgga tggcgaagga atgtatcagg
     3901 ccgatggcat ttttcttgcc catcggcggc ttgcgatcct agacctgtca gaacgaggga
     3961 aacaaccgat gtcgtatgcg gaagggcgat attggattac cttcaacggc gagctctaca
     4021 attttgtcga gattaagaac acgacggtcg gccgggaaac gaagatcgcg ca
//
LOCUS       DAOQXQ010000061         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22678_length_4048_cov_2.905835, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000061 DAOQXQ010000000
VERSION     DAOQXQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22678_length_4048_cov_2.905835"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKH81_01685"
     CDS             complement(<1..230)
                     /locus_tag="PKH81_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96777.1"
                     /translation="MPVSSEFFLPLMRGDFLAFSFFSAGHVGTPYRLSGRIMHVIGNQ
                     VKGRKNSLICDAIEASDPGYTGTTEGIEARYVS"
     gene            404..2296
                     /gene="dxs"
                     /locus_tag="PKH81_01690"
     CDS             404..2296
                     /gene="dxs"
                     /locus_tag="PKH81_01690"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNQ96778.1"
                     /translation="MNRSLLAAISSPEDLKALPSRDIPLLSQEIRKKIIEVVGANGGH
                     LASNLGVVELTLALHRVFSSPDDLIVWDVGHQCYTHKLITGRNDRFSGIRKKDGISGF
                     PKREESEHDPFNTGHSSTSISAALGILLARRQRGEKGKVIAVIGDGALTGGMAFEALL
                     QAGQGGNDLIVILNDNKMSISRNTGALSEYLSRLTMGANYQRFRRSIDRAVRVVPFVG
                     ESLTRVIFRIKRGLKGLLFKDNLFVDLGFEYVGPLNGHNVEELEKVLRNVRALHGPVV
                     VHVLTQKGRGYQLAELDPSTFHGIGPFCISDGKVEKFDTTSFTEAFSRSMVALAADNP
                     KLVAITAAMAKGTGLAPFQHRYPDRFYDVGIAEQHALTFAAGLASAGMKPVVAIYSTF
                     IQRAIDQIIHDVALQRLGVVIALDRAGAVPDDGETHQGLYDIALLRPIPSLSILTPAS
                     VVEMEAMLSWAVSQDTPVAIRYPKAACPTEEESFSLPLEVGRGVFTHRDGSDTVLVCT
                     GGMYPEVHEAATILARKGVPVDVYHLRYVKPIDEAYFLSAMEGYRRIVFVEDGVETGG
                     VCQWLESRVIKLDPSLDTHILAFDELFYPQGTRSEIIASAGLSGAHIADCVQNLRARQ
                     SAGSPS"
     gene            2293..3144
                     /gene="folP"
                     /locus_tag="PKH81_01695"
     CDS             2293..3144
                     /gene="folP"
                     /locus_tag="PKH81_01695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ96779.1"
                     /translation="MSVSIPLSGSAVLKTDLDALVMGIVNATDDSFWQGSRFPSTSQG
                     VDLALRMVEEGADILDIGAESTRPGAPYVSAEQEIERLIPLIQGIRSSSSVPISIDTR
                     KAKVFSAALEAGADMLNDVSALEDDEELAPLAARSGVPVVLMHKKGNPATMQDSPSYT
                     DVVKEVSQYLLGRARRAIEAGIHKDRIIFDPGIGFGKRLEDNRALISGLAEIVQCGYP
                     VLMALSRKSCIGAFTGRDVADRLAGTVVANVLSVQQGAKLLRVHDVRETIDMLKVYRE
                     ISGSGIH"
     gene            3131..3946
                     /gene="cdaA"
                     /locus_tag="PKH81_01700"
     CDS             3131..3946
                     /gene="cdaA"
                     /locus_tag="PKH81_01700"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNQ96780.1"
                     /translation="MEFIKKIALFYAVIQPFLDISILAFLLYVSYGILVKTQAVQLLK
                     GAVSLLIIYALAFILKLNTLLWLLNILAPGLLIGVAIVFQPELRKIFLKIGQSDWLRI
                     GKRSKHSHLDSVLTAADILSNHRRGMLAVLTRHNNLRDIVETGNRLNADLSSSLLVTI
                     FGHDTPLHDGAVIIQGGRIIAAGCFLPLSEQQDIRKTFGTRHRAALGLSEETDAVILI
                     VSEETGAISLAYDSKLYYDLSSAEITRQLEGLLELGSETAGGGDQGNESKDFD"
BASE COUNT          948 a          950 c         1030 g         1120 t
ORIGIN      
        1 gaaacgtacc ttgcttcaat tccttcggtg gttccggtat atccgggatc cgaggcctcg
       61 attgcatcgc agattaagga gtttttgcgc cccttgacct gatttccgat aacgtgcata
      121 attctaccac ttaatcgata aggagtgccg acgtgccctg cggaaaaaaa cgaaaacgcc
      181 agaaaatcgc cacgcataag cggaagaaaa aactcagaag aaaccggcac aagaagaaat
      241 aatctactac cgggcttatt gccggggagg tttctttatc aggggtattg gcagtccggt
      301 gtaccggtca gccagtcctc gcggagcgca agcgaaaggt aagaccgtaa acactttgtt
      361 tacggtcttt ttttttctac ttcgctatgg tatagttttt tccatgaatc gatccctcct
      421 ggcagctata tcatcgccgg aagaccttaa ggcccttcct tcccgggaca tcccccttct
      481 ttcacaggaa attcgcaaga aaattattga agtggtaggg gcaaacggcg gacacctggc
      541 aagtaatctg ggtgtggtag aactgaccct tgctcttcat cgtgtgtttt ccagtcctga
      601 cgatttgatt gtgtgggatg ttggccatca atgttatacc cataagctta ttaccggccg
      661 caatgaccgg ttttcgggta ttcgcaaaaa agatggtata tcaggctttc ccaaacggga
      721 agaaagcgag catgacccct ttaacaccgg tcattcttct acctctatat cggccgccct
      781 tggaatactg cttgctcgca gacagcgggg cgaaaaaggg aaggtcattg ccgttattgg
      841 cgatggtgcc ttaaccggcg gaatggcctt tgaagcgctg ttacaggctg gtcagggtgg
      901 taacgatcta atcgtcattc ttaacgataa caagatgtct atcagccgca acaccggagc
      961 tctttcggag tatttaagcc ggctaactat gggcgccaat taccagcgtt ttagacgaag
     1021 tatcgatcgt gcagtccgcg ttgttccctt tgtgggtgaa tcgttaaccc gggtaatttt
     1081 ccggattaaa cgcggtctta agggtttgct cttcaaagat aatctttttg ttgaccttgg
     1141 gtttgaatat gttggacctc ttaatgggca taatgtagag gagctggaaa aggttctccg
     1201 taatgttcgt gcgctccacg gtcctgtggt ggttcatgtc ctgactcaaa agggaagagg
     1261 atatcagctt gccgagcttg atccgtcaac ttttcatggt attggcccct tttgtatcag
     1321 tgatggtaag gtagaaaaat tcgacaccac cagttttacc gaagcttttt cccgaagtat
     1381 ggtcgctctg gccgctgata atcccaagct ggtagcgatt acggctgcca tggccaaggg
     1441 tactggactt gctccctttc aacatcgcta tcccgaccgc ttctatgatg tgggcatagc
     1501 tgaacaacat gcgcttacct ttgccgccgg actcgccagt gcggggatga aaccggttgt
     1561 tgccatatat agcaccttta ttcagcgggc tatagaccag ataattcatg atgtggcctt
     1621 gcaacggctt ggagtagtta ttgccctgga tagagccgga gctgttcctg atgacggaga
     1681 gactcatcag ggactctacg atatcgcctt gctcagaccg atcccctcgc tttcgattct
     1741 tacaccagct tcggttgtag agatggaagc gatgctaagc tgggcggtca gtcaggacac
     1801 cccggtggca atacgatatc ccaaggctgc ctgtcctacc gaggaagaat ctttttctct
     1861 tccactggaa gtcggccgtg gcgtatttac ccatagggat ggctcggata ctgtcctggt
     1921 gtgcaccgga gggatgtatc ctgaggtaca cgaagctgct acgattctcg ctcgtaaggg
     1981 tgtgcctgta gatgtgtatc atcttcgtta tgtaaaacca atcgatgaag cgtattttct
     2041 ttccgcaatg gaagggtata gacgaattgt gtttgtcgaa gacggggtag aaacaggtgg
     2101 cgtctgtcag tggctggaaa gccgtgttat caagctcgat ccatcactcg acactcatat
     2161 tctcgctttt gacgagttgt tttatccgca gggcacccgt tcggaaataa ttgcttctgc
     2221 aggtttatca ggcgcacata ttgctgactg tgtacagaat cttagagccc gtcaatctgc
     2281 aggttctcct tcatgagcgt atcaattcct ctttccgggt ccgcggttct taaaacggat
     2341 cttgatgccc tggttatggg tattgtaaac gcaaccgacg attctttttg gcagggaagt
     2401 cgcttcccgt ccacctcgca gggggtagac cttgcccttc gcatggtgga agaaggtgcc
     2461 gatatactcg acattggcgc tgaatcgacc aggcccggcg ccccgtatgt gagcgccgaa
     2521 caggagattg agcggcttat cccccttatt cagggtattc gctcatcgag tagtgttccc
     2581 atctcgattg atacccgaaa ggccaaggta ttttctgctg ccctggaagc gggtgcggat
     2641 atgctcaatg atgtatccgc cctggaagat gatgaggaat tagcgccact ggcagcccga
     2701 tctggtgttc cggttgttct tatgcataaa aaaggaaatc ccgcgactat gcaagacagt
     2761 ccttcgtata ctgatgtggt taaggaggtc tcgcagtatc ttcttggccg cgcgcgccgg
     2821 gccattgaag cggggattca caaggatcgc attatttttg atccgggaat cggctttggt
     2881 aaacgacttg aagacaatcg tgcgctcatt tcaggccttg cggaaatcgt ccagtgcgga
     2941 taccctgtac ttatggccct gtcgcgaaaa agttgcattg gagcgtttac tggcagggat
     3001 gttgcagacc gtttagcggg aactgttgtc gccaatgtgc tgtctgttca acaaggcgca
     3061 aagctcttac gcgttcatga tgtgcgcgaa actatagaca tgctaaaagt gtatcgggag
     3121 atctctggca gtggaattca ttaagaaaat cgcgctcttt tacgccgtaa ttcaaccctt
     3181 tctggatatc tccatccttg cctttctttt atatgtttcg tatgggattc tggttaaaac
     3241 acaggctgtt cagctactaa aaggcgcggt gtccctgctt atcatctatg cccttgcctt
     3301 tattcttaaa ttaaacacac tactgtggct gttaaatatt cttgctccgg gattactcat
     3361 cggtgtggcg attgtgtttc aacccgagct tagaaaaatc tttctcaaga tcggtcaaag
     3421 cgattggttg cgtatcggta aacgatccaa gcacagtcat cttgattctg ttcttaccgc
     3481 ggccgacatc ttgtccaatc atcgcagggg aatgcttgcc gtgctaaccc ggcataacaa
     3541 ccttcgcgac atagtagaaa cgggcaatcg attgaatgcg gacttgtctt caagcctttt
     3601 ggtaacgata ttcgggcacg atactccgct tcatgatgga gcggtaatca ttcagggtgg
     3661 caggattatt gcagctggat gttttttacc cctttccgaa caacaggata tccgaaaaac
     3721 gtttggaacg cgacatcgtg cagccttggg tctttctgaa gaaacagacg cggtaatcct
     3781 tatcgtatcc gaggaaactg gtgcaatcag cctcgcctat gattcaaagc tatattatga
     3841 tctatcttcc gcggagatta cccgccagtt ggaaggtctg cttgaattgg gtagtgaaac
     3901 agccggagga ggggaccagg gaaatgaaag caaagatttt gattgatcgt atcctggata
     3961 actggccggc aaagattgta tgtctgactc tttctctatt gctctttatg ttttatcgta
     4021 tgagtacact cgagaaacga tatttttc
//
LOCUS       DAOQXQ010000062         4008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_22985_length_4008_cov_3.252466, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000062 DAOQXQ010000000
VERSION     DAOQXQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22985_length_4008_cov_3.252466"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKH81_01705"
     CDS             complement(<1..888)
                     /locus_tag="PKH81_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNQ96781.1"
                     /translation="MNVVVIGAQWGDEGKGKIVDYLAADSQCIVRFSGGANAGHTIVS
                     DGVQYALHLVPSGILHANNTVYLGAGMVIDPEALFKELAMLTEKGIAWEGRVFISDRA
                     HLVLPRYRELDKVRDAARKRPIGTTGRGIGTTYALKSERDGIRLADLTWKVKMADLEE
                     EDRAFLSPYVDRLLAMKVNLTTELWKQRGKKILFEGAQGALLDLDLGTYPYVSSGFSS
                     SAGAAIGGCVGPRSIDRVLGVFKAYSTRVGNGPMPTEFCANSQSELYTFVRETGREYG
                     VTTGRPRRCGYLDLVALRYA"
     gene            complement(899..2344)
                     /gene="guaB"
                     /locus_tag="PKH81_01710"
     CDS             complement(899..2344)
                     /gene="guaB"
                     /locus_tag="PKH81_01710"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNQ96782.1"
                     /translation="MMEMKETLSYDDVLLEPGYSEVLPRDTDVSTVLVRDVRLNVPVI
                     SAAMDTVTEEKMAVALALEGAAGVIHRNLHPEVQAEQVSNAKRYLNWVIESPVTVEDD
                     RTIGDVKQIMHRYNVSGLPVITNEGKLVGIITGRDLRFCRDDSLFVRDVMTKDLVVTE
                     GKPSTDDARQKFDRHRIEKLPVIDHGGHLTGLITVKDMEKQEMFPYAARDKKGRLIVG
                     AAVSNNDWSVRIPLLMEAKVDFVVLDTAHGDSKGVIEAVRSIKKAFPDLPVIGGNVAN
                     AAGTRHLIEAGADAVKVGVGPGSICTTRIVSGVGAAQFSAVCDCAEEASKSNIPIIAD
                     GGIKFSGDISKAIGAGANVVMIGNLFAGLKEAPGREIIFDGRIYKEYRGMGSLGAIKD
                     GSGDRYGISKDESPVPEGIEGRVPYKGELKSFLHQMVSGLRKGMGYCGCKSIEELKKY
                     RKFVRITTSGLRESHAHDVTITQEAPNYSRY"
     gene            complement(2346..3773)
                     /locus_tag="PKH81_01715"
     CDS             complement(2346..3773)
                     /locus_tag="PKH81_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNQ96783.1"
                     /translation="METRTIFLNISPIDHRYSLSESSVYERLSSFISEQAAIVSCARA
                     EIALIKAHLAIRGQLTEARDKELDSVADRIDPSAVYAEEEKTKHNIRALVNVLKTMVS
                     ADIAPLVHLGATSVDILDTALSVRVRDCVQSVILPELRLLETALCTIAAREAKTPQVG
                     RTHGQHAVPITFGFAISEYVSRLGKSILEIEARSNQLAGKLAGAVGAYNATSMIVRDP
                     EELERKYLSYLGLVPSEHSTQLVEPEYLLRLLLELNVAFGVIANLADDLRNLQRSELG
                     EVFEYFSSTQVGSSTMPQKRNPWNSEHVKSLWKAFAPRVMTFFMDQISEHQRDLTNSA
                     SQRFVADYLAGFTLAVSRMKSVVSGVQADRDGMARNLSNAGGKVSGGVLAEPAYILLA
                     ESGISDAHEVIRTITLDAENTNCTFFEALSRNQDAYTRITSQLEKLGIDDAPGFFANP
                     ERYRGLAAEKAARLAAKWKTLMEEK"
     gene            3827..>4008
                     /locus_tag="PKH81_01720"
     CDS             3827..>4008
                     /locus_tag="PKH81_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNQ96784.1"
                     /translation="MMFFLVKLRETALAVLPIVLLVAILSLTVAPLPKGLLVVFLRDS
                     LLLIAGLTLFLTGAEVG"
BASE COUNT         1014 a         1079 c          937 g          978 t
ORIGIN      
        1 ggcataacga agggcaacca aatcaagata accacagcga cggggtcgtc cggtggtaac
       61 gccatactcg cgtccggttt ctcgaacaaa ggtatacagt tcactctggc tgttcgcgca
      121 gaattctgtt ggcatcggac cattacccac acgagttgaa tacgccttaa atacaccaag
      181 gacacggtca atcgaacggg ggccgacaca tccaccaatg gcagcgcctg ccgaagaaga
      241 aaagcctgaa gaaacgtagg gataggttcc caaatcaaga tcaagaagcg cgccctgggc
      301 accttcaaac aagatcttct ttcctcgctg cttccacagc tcggttgtca ggttaacctt
      361 catagcaagt aagcgatcta cgtatggaga gagaaaggcg cgatcttctt cttcaagatc
      421 ggccatctta accttccagg taagatcggc aaggcggata ccgtctctct cgcttttaag
      481 ggcgtatgtg gtaccaatgc cccggccagt ggtcccgatc gggcgtttgc gcgccgcatc
      541 acgaacctta tcaagctcgc ggtagcgggg cagcacaaga tgcgcgcgat cggaaataaa
      601 aacgcgacct tcccaggcaa tacctttttc ggtaagcatg gcaagttcct taaacaaggc
      661 ctcaggatca ataaccattc cggcgccaag gtataccgtg ttgttggcat gaaggattcc
      721 ggatggaacc aagtggaggg catattgcac gccgtccgag acaatcgtgt gtccggcatt
      781 ggcgccgccg gaaaaacgca cgatacactg ggaatccgcg gccaaatagt ccacgatctt
      841 cccctttccc tcgtcacccc attgggctcc gataacaaca acgttcatgt ctacctactt
      901 aatagcggga atagttcggg gcctcctgcg tgatcgtaac atcatgcgca tgactctcgc
      961 ggagtcccga tgtggttatc ctaacaaatt tccggtattt cttcaattcc tcaatgctct
     1021 tacaaccgca atatcccata ccttttcgta aacctgacac catttgatga agaaaactct
     1081 ttaactcgcc cttataggga acacgtcctt caattccttc cggaacaggg ctttcgtcct
     1141 tgctaatccc gtaacgatct ccggaaccgt ccttgatcgc tccaagacta cccattccgc
     1201 gatattcctt ataaatgcgt ccgtcaaaaa taatctcgcg tccaggagcc tcttttaagc
     1261 cggcaaacag gtttccaatc attaccacat tcgcaccagc gccaattgcc tttgaaatat
     1321 cgccggagaa cttaataccg ccatcggcga taataggaat gtttgacttt gatgcctctt
     1381 cggcgcaatc gcacacggcg gaaaactggg cagctcccac accggatacg atgcgggttg
     1441 tgcagataga accgggtcct accccaacct ttaccgcgtc tgctcctgcc tcgattaaat
     1501 ggcgggttcc cgcagcattc gctacgtttc caccaataac gggtaaatcc ggaaaggcct
     1561 ttttaatgga acgaacggct tcgataacgc cttttgaatc tccatgggcg gtatcaagga
     1621 ctacaaagtc taccttggct tccatcaata aggggattcg cacagaccaa tcattattgc
     1681 tcaccgcagc ccctacaatc aggcggcctt ttttatcgcg ggccgcatag ggaaacattt
     1741 cctgcttctc catatccttt accgtaatca gcccggtaag gtgcccacca tgatcgataa
     1801 cgggaagttt ttcgatgcga tggcgatcaa acttttgtcg tgcatcgtcg gtactcggct
     1861 ttccttcggt aacgaccaaa tccttggtca ttacgtccct gacaaacaaa ctgtcatcac
     1921 ggcaaaaccg aaggtccctg ccggtaataa taccaacgag ctttccctca ttggtaatta
     1981 ccggaagccc ggaaacatta taccggtgca ttatctgttt tacatcgccg atcgttcggt
     2041 cgtcctctac agttaccggg gattcaatca cccagttaag atatcgcttg gcgtttgaaa
     2101 cctgttcagc ttgaacttct ggatggagat tccggtgaat aactcccgcc gcaccttcca
     2161 gggcaagggc cactgccatt ttttcttcgg ttaccgtatc catcgcggca gagataaccg
     2221 gcacattgag ccgtacatcg cgaacaagaa cggtgcttac gtcggtgtct cttggcaata
     2281 cctctgaata gcccggctca agaagcacat cgtcgtacga cagggtttct ttcatttcca
     2341 tcatattatt tttcctccat tagcgttttc cactttgccg ccaggcgcgc ggccttttct
     2401 gccgccaaac cccggtagcg ctcggggttc gcaaagaaac cgggagcatc atcaatgcca
     2461 agtttttcca gctgagaggt aatgcgcgta tacgcatcct gatttctgga cagggcttcg
     2521 aaaaaggtac aattagtgtt ttccgcatcc agggttatcg ttctgataac ctcatgagcg
     2581 tccgagattc ccgattctgc aagaaggata tacgcgggct ccgcaagaac accgcctgac
     2641 accttgcctc cggcattgga aaggtttctg gccataccat cacgatctgc ctgaacgccg
     2701 gagacgacac tcttcattcg cgacacggcc agggtaaagc ccgcaagata gtcagctaca
     2761 aagcgttgac ttgccgaatt ggtcaggtcc cgctgatgtt cggaaatctg atccataaaa
     2821 aaggtcatca cgcggggagc aaaggctttc cagagacttt ttacatgctc tgaattccag
     2881 ggatttcgtt tttggggcat cgtggaagaa ccaacttgcg tcgagctgaa gtattcaaat
     2941 acctcgccaa gctcggaacg ctgaaggttt ctcaaatcat cggcaaggtt cgcaataaca
     3001 ccaaaggcaa cattgagctc aagaagcagg cgcaacagat actcgggttc aacaagctga
     3061 gtagagtgtt cagagggaac aaggccaaga taggaaagat actttctttc aagttcttcc
     3121 ggatcgcgaa caatcatgct cgtggcgtta tatgcaccta ccgctccagc gagttttccg
     3181 gcaagctgat tagatcgagc ttcaatttcc aatatcgatt ttcccaaacg gcttacatat
     3241 tccgatatgg caaaaccaaa ggtaatggga accgcatgtt ggccatgagt gcggcctacc
     3301 tgcggggtct tcgcttcccg tgcggctatt gtacaaagag cagtttccaa aagccgaagc
     3361 tcgggaagaa tgacagactg aacacaatcg cgaacccgta cggaaagtgc ggtatcaagt
     3421 atatcaacgc tcgtagcacc caaatgaacc agaggggcta tatccgcaga aaccattgtt
     3481 ttaagcacat taaccagggc acgaatattg tgtttggttt tttcctcttc tgcatatacg
     3541 gcagaaggat caatgcggtc cgctaccgaa tcaagctctt tatcgcgagc ctcggtcaat
     3601 tgccccctta tggctaaatg agccttgata agggctatct cggcccgagc gcaggagaca
     3661 atggcagcct gttcagaaat aaaagaagac agtcgctcgt aaacggagga ctccgaaagt
     3721 gaatagcggt gatcgattgg ggagatattg agaaagatag tacgtgtttc catgctcaat
     3781 attgccagaa tcttgttggt actgtcaaga tatgctagta tccgtcatga tgttttttct
     3841 ggttaaactt cgggaaactg cccttgctgt cttacccatt gtcctgctcg tggcgatcct
     3901 gtcgctcacg gtagcaccct tacccaaggg attattagtg gtgtttctcc gagattccct
     3961 gttactcatc gcaggtctca ccctttttct aaccggtgcg gaagttgg
//
LOCUS       DAOQXQ010000063         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_23493_length_3946_cov_2.872269, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000063 DAOQXQ010000000
VERSION     DAOQXQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23493_length_3946_cov_2.872269"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKH81_01725"
     CDS             complement(<1..378)
                     /locus_tag="PKH81_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96785.1"
                     /translation="MCIIWHVTSISINEPFILPDPLTVFFDALLLFTDPLFFMEAWAT
                     CIRSLLAFVISVSLSLILGIPSGLDTRIEYALKPPLALIKATPVVSFILIALLWFGSS
                     IVPIFVSVLMTLPIMTEAILKGMK"
     gene            complement(420..1403)
                     /locus_tag="PKH81_01730"
     CDS             complement(420..1403)
                     /locus_tag="PKH81_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ96786.1"
                     /translation="MKRITITFLCICIASQTFSMGTKEGIQEQPSSIHIAALRGPSAL
                     GMSRLFETGYSINTIALTMQIASSIDAVYPKLLNGDIDIAILPPNVAAKLYNKNPSSV
                     IALAVVGNSMLSLLSRDPTIQSPTDLSGKKVYVAGQGATPDYILRTLTTENLSDVSKV
                     QYDYSLSTADIATAIATGRIDYAFLPEPFVTLSMEVAKEKNNPLTISFSAQDSWAASG
                     KGDDFPMTVCVVRKEFAKQHDSLVRDFLEAYRLSVQWTLDNPKRAGTLAEKAGIGLQA
                     AVAEKAIPRCNLVFIPANEARGSLERLLQIFLLFDSQSIGGRLPDDGFYFQ"
     gene            complement(1567..>3946)
                     /gene="ppdK"
                     /locus_tag="PKH81_01735"
     CDS             complement(1567..>3946)
                     /gene="ppdK"
                     /locus_tag="PKH81_01735"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNQ96787.1"
                     /translation="VEGLAKKTGNRRFAMDAYRRFIMMYGSTAMGICREKFDHEFDTL
                     KNNVTKKRLGLKKDEKVADTDVNDEELALLIQTFKSLYKKEIGKPFPQDPVDQLWGSI
                     GAVFGSWMADKAVTYRKVEKLFGVKGTAVNIVQMVFGNMGDTSGTGVCFTRDPNTGEN
                     VFYGDYLINAQGEDVVAGIRTPIKLSDFAKKDPKAYKQLCNVRSILEKHYKDMQDLEF
                     TVEEGTLYMLQCRTGKRSPAAAFKIACDLVNEKLITKEIAVSRIKTSDIEGIFYPTID
                     KSQAKELKASLIAQGIDAVPGAACGKVCFSAEAAELLAERGEKVILVRKETSPEDIGG
                     MHAAQGILTATGGKTSHAAVVARGWGKCCIVGCEKLNISYEKKRFVAGGTTILEGEYI
                     TLDGSNGCVYKGELKLVAPKLPSAYNTIMNWVDELKKIKVRTNADTPYDAVKARGLGA
                     EGIGLCRTEHMFFDTEERILAIREMIIAESVDVRKKALAKLLPFQTKDFEGIFKAMDG
                     LGVTIRLIDPPLHEFVPHDIAGQKALAQATGVSLATVTDRIEKLHEANPMLGHRGCRL
                     AITYPEILEMQVTAIMTAACRMAKKGIKVFPEIMIPLVIDAKELKILEELTRKTADSV
                     ISRLGVKVSYLVGTMIETPRAALLAHEIAEVAEFFSFGTNDLTQMTLGMSRDDSGKFL
                     PEYIDEHKAAVFRVDPFQTLDQAGVGLLVKSGIEKGRSIRGKLKVGICGEHGGDSESV
                     KFCARAGMDYVSASPFRVPVARLAAAHAAIEDAAARKKPVASKAKKASTAKK"
BASE COUNT         1079 a          890 c          821 g         1156 t
ORIGIN      
        1 tttcattccc ttaagaatcg cctcggtcat gataggcagg gtcatcaata ccgaaacaaa
       61 tatcggtaca atcgaagacc caaaccacaa taatgcgatt agaataaagg agacaactgg
      121 agtcgctttg atcagggcta gcggaggttt aagagcatat tctatgcggg tgtcaagacc
      181 agatggaatc ccaagaataa gcgaaagact tacagaaatc acgaatgcta aaagacttct
      241 tatgcaagtt gcccaagctt ccataaaaaa aagtggatca gtaaatagaa gcagtgcatc
      301 aaagaacact gtcagcgggt caggcaaaat aaaaggttca ttaatggaaa tcgaagttac
      361 atgccatatt atgcacataa tgagtatcga aagtgtcagg gataaactgt gccttttttt
      421 cattggaaat aaaagccgtc gtcaggtaga cgtcctccaa tagactggga atcaaatagt
      481 aaaaagattt gcagtaggcg ttccagggac cctcgagcct cattggcagg aataaatacc
      541 agattacaac gaggaatagc tttctctgct acggctgcct gtaatccaat tccggctttt
      601 tctgctaaag ttcctgctct cttgggatta tcaagcgtcc attgaactga gagccggtat
      661 gcttccaaga aatctcgtac caaagaatca tgctgctttg caaattcctt tcggacaaca
      721 cagacagtca tcggaaagtc atctcctttt cctgaggcag cccaactatc ttgagcggaa
      781 aatgagatag taagaggatt attcttttct tttgctactt ccatggaaag tgttacgaat
      841 ggttcgggta aaaatgcata gtcgattcgt cctgttgcta tagctgtggc aatgtcagct
      901 gttgataagg aatagtcata ctgtaccttg gagacatcag aaaggttctc tgttgtaaga
      961 gttcttagaa tataatccgg cgttgcgccc tgccccgcta catacacctt tttgcctgat
     1021 aaatcagttg gactttgtat cgttggatcg cgcgagagaa ggcttagcat actgttccca
     1081 actactgcta aggcaataac tgatgaagga tttttattat agagttttgc ggcaacatta
     1141 gggggtaaaa tggcaatatc aatatcacca ttaagtaatt tcgggtatac ggcgtcaata
     1201 gaagatgcta tctgcattgt cagggcaata gtattgatac tataaccggt ttcaaagaga
     1261 cgggacattc caagggcaga aggccctcta agggcagcga tgtgtataga cgatggttgt
     1321 tcttgtatac cctcttttgt gcccatggag aaggtctggc tcgcaatgca tatacataaa
     1381 aaggtaatag taattcgttt catggacttg attatatctg tttattgcat tttttttcca
     1441 gtagacataa ggacatcgca accttgccaa atccactggt atacatattt atacatactc
     1501 ataacgtttc tagggcatgc ataagttggt aaagatggca gacatgtctg ccatcttcat
     1561 gattttttat ttttttgcgg tagaagcctt ctttgctttg gatgcaaccg gtttcttcct
     1621 ggcagcggca tcttcaattg ctgcgtgagc agcagccaga cgagctaccg gaacgcggaa
     1681 cggtgaagcg gaaacataat ccattcctgc tcgtgcgcag aattttaccg actcagaatc
     1741 ccctccatgt tcaccgcaga tgccaacctt cagttttcct ctaatggatc gacccttctc
     1801 tataccggat ttaactaata agccaacgcc ggcttgatcc agggtttgga aaggatccac
     1861 acggaagaca gccgccttat gctcatcaat gtactctgga aggaatttgc ctgaatcgtc
     1921 ccggctcata cctaaagtca tctgagttaa atcatttgtt ccaaaggaga agaactccgc
     1981 aacttccgct atttcatgcg ctaaaagagc tgctcggggt gtttctatca tggttccaac
     2041 caggtaagag accttaacac cgagtcgaga aataactgaa tcggcagttt tacgagtaag
     2101 ttcttccaga atctttagtt cctttgcatc aataacaagg ggaatcataa tttcagggaa
     2161 caccttaatg ccttttttag ccattcggca ggcagcggtc attattgcgg taacttgcat
     2221 ctcaagaatc tcaggatacg ttatggctaa tcggcatccg cgatggccaa gcatagggtt
     2281 tgcctcatgt agtttttcta tccgatcggt taccgtagca agacttaccc cggttgcctg
     2341 ggcaagagct ttctgtccgg ctatatcgtg gggaacaaac tcgtgaagag gaggatcaat
     2401 gagacgtatg gtaaccccaa gaccatccat tgctttaaaa ataccctcaa aatcctttgt
     2461 ctggaaaggc aagagttttg ccagcgcttt tttacgtacg tctactgatt cggcgataat
     2521 catttctctg attgccagta tccgttcttc ggtatcaaag aacatgtgtt ctgttcggca
     2581 caatccaatc ccttcagctc ccagaccacg ggccttaact gcgtcataag gggtgtctgc
     2641 attagtcctg acctttatct ttttaagctc atcgacccag ttcataatcg tgttataggc
     2701 ggagggaagt ttgggcgcta cgagtttgag ttcaccctta tatacacatc catttgatcc
     2761 gtccaaagtt atatactcgc cttccaggat tgtggttcca ccggcaacaa aacgtttttt
     2821 ctcataactg atattgagtt tttcacatcc aacgatgcag cacttacccc aacctcgggc
     2881 aacaacagct gcgtgacttg tttttcctcc agtcgcggta aggattccct gtgcggcatg
     2941 catgccaccg atatcttcgg gacttgtttc ctttcgcaca aggataacct tctctcctcg
     3001 ttcagccagt aattctgctg cttctgccga gaaacaaact ttaccacaag cggctcccgg
     3061 aactgcatca attccttgag caattaaact agccttaagt tcttttgcct gtgacttgtc
     3121 tatagttgga taaaatattc cttcaatatc acttgttttg atgcggctta ctgcgatttc
     3181 ttttgtaatg agtttttcgt tgacaagatc acatgctatc ttgaaagctg ccgccgggga
     3241 acgtttccct gttcgacatt ggagcatata gagcgttcct tcttcaacgg taaactcaag
     3301 atcttgcata tccttataat gtttttccag aattgaacgg acattacaca attgcttgta
     3361 ggccttggga tcttttttag caaaatcaga gagcttgatt ggtgtgcgaa tacccgcaac
     3421 aacatcttct ccctgagcat tgataagata atcgccgtaa aaaacatttt ctccagtgtt
     3481 tggatcgcgg gtaaaacata ctcctgttcc tgatgtgtct cccatattcc cgaacaccat
     3541 ctgaacgata tttactgcgg tgccttttac accaaacaat ttttctacct tgcggtacgt
     3601 aacggccttg tctgccatcc atgaaccaaa aacagcaccg attgatcccc atagttgatc
     3661 aacaggatct tgcggaaaag gttttccgat ttctttttta taaaggcttt taaatgtttg
     3721 aattaacagg gcaagttctt cgtcattaac atctgtatca gcaactttct catctttttt
     3781 taagccaagc cttttttttg tgacgttatt cttaagtgtg tcaaattcat gatcgaattt
     3841 ttctctgcaa atccccatag cagtagaacc atacatcatg ataaaacgtc gatacgcgtc
     3901 catggcaaag cgccgattac cggttttctt agcaaggcct tcaaca
//
LOCUS       DAOQXQ010000064         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_23628_length_3931_cov_3.038442, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000064 DAOQXQ010000000
VERSION     DAOQXQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23628_length_3931_cov_3.038442"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1006)
                     /locus_tag="PKH81_01740"
     CDS             complement(98..1006)
                     /locus_tag="PKH81_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNQ96788.1"
                     /translation="MEDQAEKLRTLMKEKTQAQGSAPEMHPDSDLRSNRKTRIITVTS
                     GKGGVGKTNVATNMAIAYAQMGKKVIVIDADLGLANVNVMMNLIPQYNLYHVMRKQKK
                     MSEIILDTDFGIKLVAGASGFSKIANMSEDERNSFIGELYTLSDTDIIIIDTSAGVSK
                     NVLGFVAAADEVVIVTTPEPTSITDAYGIIKIIATEVDNLNINLKMIVNRVGSALEGK
                     RVADRMTQIAAQFLNLKVEYLGFIYDDPVVSQSVLRQKPFIIVDPKCKAAMCLKHIVS
                     RIEKTEFPETEGFGRFMRKLFGHQWG"
     gene            complement(1008..2291)
                     /gene="flhF"
                     /locus_tag="PKH81_01745"
     CDS             complement(1008..2291)
                     /gene="flhF"
                     /locus_tag="PKH81_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNQ96789.1"
                     /translation="MDILSEQGTTYDECLEKIRNRHGPNVHILRQKKVRLGGFLGFFE
                     KDGIELYYMLSTNTLRSSAPAPQRHQSFDEERKKIIRQAVESAPSIASRVPPITLSSQ
                     STPSPSSDRGQDQLETILKTVKNLEARIAGKADTDQSTDEHETITRIETLLELNDFSP
                     SYIKKIVARLRSDFSLEELSDFDAVQDAVIQWIGTSVSIADLGATSRPRIIVLVGPTG
                     VGKTTTVAKLAAAYSLAALKGSRPLNVRVITIDNYRIGAKQQIEIYGNIMSIPVSCAE
                     TPSDLQALVAMYRDVDVILIDTIGKSPKDYARIAEMRHFLDAAGPLSDVHLAVSATTK
                     ASDMREILQQYETFGYGSLIVTKFDETEHVGNIISILDEKKKALAYITTGQRVPQDFE
                     IASTVRLLTNLEGFRVNRSRIEEEFPVSDTRFEWR"
     gene            complement(2321..>3931)
                     /locus_tag="PKH81_01750"
     CDS             complement(2321..>3931)
                     /locus_tag="PKH81_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNQ96790.1"
                     /translation="EAEYNAGAITEEESRARKAEVQKEADFYGAMDGASKFVSGNVKI
                     GIFITVINLVAGLIFGMIFRGEPFGLAIQTYAGLTIGDGLLSQMPSLFVSVATGLIVT
                     RAVSDGTFGSDIQTQFSRNAWVYYVGAGTLLFISFLPGFPWYVLIPVSAGLAWVGWRL
                     RKAEARTFKKTLEREEEKEKQKTGENPADISPVVPLDPLSLELGYGLIPLVDKDKGAE
                     LLERVTRIRRESALDLGLVVPRIRIIDNMRLEPSEYCFKIKGVEVARGRIRMGWFLGI
                     NPGGASEDLPGEKTVDPAFGLPAIWISEDNREKAERAGYTVVDPPSIIATHLTEVIKR
                     HASEILGRQEVQAIVDTIRKDYPAVVEEVVKLCSVGEIQKVLQALLREQVSIRNMVVI
                     LETLADFRPITKDSTILVEKVRQALSRQICLQYTDDQSTLHVLTVEPSLVQRIVDSRL
                     DTVNGPIAALEPAVQRSWIRSLTRSVASVQQSGFLPIILCPEEARILIKSSTEREIPD
                     LVVLSVPEIAKDIKVEAIGEIKTEEAAS"
BASE COUNT         1017 a          942 c          891 g         1081 t
ORIGIN      
        1 gttcgaacat gaatattccc tcccagctac tagactatgc gaaaaagtgt tactggtcaa
       61 ggtttttttt ggatgtacct gtgtcgagag cggagactca tccccattga tgaccaaaaa
      121 gtttgcgcat aaagcggcca aaaccttcgg tctcgggaaa ctcggttttt tcgatacggg
      181 atacaatatg cttaagacac atagccgcct tgcattttgg atccacgatg ataaagggtt
      241 tttgcctgag tacagactga gatacaaccg gatcatcata gataaaacca aggtattcaa
      301 ccttaagatt taagaattga gcggcaattt gagtcatgcg atctgcaact cgcttgcctt
      361 ccagggcgct tcctacccgg tttacaatca ttttaaggtt tatgttcaag ttatctactt
      421 ctgtcgcgat aatcttgatt attccatagg cgtcggttat ggatgttggt tccggggttg
      481 taacgataac aacctcatca gcggcagcga caaagcccag tacgtttttg gacacccctg
      541 cactggtgtc aataataatg atatcggtgt ccgaaagggt atataactca ccgataaagg
      601 agtttcgctc gtcttcgctc atatttgcaa ttttagaaaa acccgaagca ccggcaacaa
      661 gcttgatgcc aaaatcggta tccaaaataa tctcgctcat ttttttttgc tttcgcatga
      721 catgatagag gttatactga ggtatcaggt tcatcataac gttgacgttt gcaagaccaa
      781 ggtcggcatc aatgacaata accttcttac ccatctgggc atatgcgatt gccatatttg
      841 ttgcgacatt tgtctttcct acgccgcctt ttccactggt aacagtaata atgcgtgttt
      901 ttcggttaga cctaaggtca gaatcaggat gcatttctgg tgctgaacct tgtgcctggg
      961 ttttttcttt cataagggtg cgaagtttct ccgcctgatc ttccatgtta tctccattca
     1021 aaccgggtat cgcttaccgg gaattcttcc tcgatacgac tgcgatttac gcggaatcct
     1081 tcaagattcg tcaataacct caccgtggaa gctatctcga agtcctgtgg aaccctctga
     1141 ccggttgtta tataggctaa ggccttcttt ttttcgtcaa gaatactgat gatattccca
     1201 acgtgttcag tttcgtcaaa ttttgtcacg ataagcgatc catacccaaa ggtctcatac
     1261 tgttgaagta tctctcgcat atcgctcgcc tttgtagtgg cgcttactgc taaatgcaca
     1321 tcagaaagcg ggcctgccgc atccaggaaa tggcgcatct cggcaattct tgcataatcc
     1381 ttaggactct ttccgatagt atcgataaga ataacgtcaa catcccggta catggcaaca
     1441 agcgcctgta aatcggaggg tgtttcggcg caggagacgg gaatactcat gatatttccg
     1501 tatatctcta tctgttgctt ggccccaata cggtaattat cgatcgttat gacgcgaaca
     1561 ttgagcggtc ttgaaccttt taacgcggca agtgaatagg cagcggcaag ttttgcaacg
     1621 gtagtcgttt ttccgacccc ggtaggtcct acaagcacga taattctggg ccgtgatgtt
     1681 gcaccaagat ctgcgataga cactgatgtg ccaatccatt ggataaccgc atcctgtaca
     1741 gcatcaaaat ctgaaagttc ctctagcgaa aagtcggatc gcaaacgagc gactattttt
     1801 tttatgtagg atggagaaaa atcattgagc tcgagtagtg tttcgatacg ggtgattgtc
     1861 tcatgctcat cagtcgattg atcggtgtct gctttgccgg caattcttgc ttcaagattc
     1921 ttgacggttt ttaatatcgt ttccagttga tcctgccccc tgtccgatga aggagaagga
     1981 gtcgattgac ttgaaagagt gatggggggc acacgggaag cgatggaggg agcgctttct
     2041 actgcttggc gaattatttt tttgcgctct tcatcaaaac tttgatggcg ctgaggagct
     2101 ggggcagaag agcgcaaggt atttgtagaa agcatgtaat acagctctat accgtccttc
     2161 tcgaaaaatc cgagaaaacc tcctagacga actttttttt gcctgagaat atgaacattg
     2221 ggaccgtgcc tgttacggat tttttcgaga cactcgtcgt aagttgttcc ctgctcactc
     2281 aatatatcca tttaacccca ccagattatg cgcttggcgt tcaggacgcc gcttcttcag
     2341 tttttatctc accaattgcc tctaccttga tatctttagc aatctcggga actgataata
     2401 cgactaagtc gggaatctcc cgctcggtcg agcttttgat aagtattctt gcttcttccg
     2461 gacaaaggat tatcgggaga aagccagatt gttgtacgga tgccaccgaa cgggtaaggg
     2521 agcgaatcca ggatcgctgt acagccggtt caagggctgc aattggaccg ttaacggtat
     2581 caagacgact gtcaacaata cgttgaacaa gcgaaggttc aaccgtaagc acatgtaaag
     2641 tggattgatc gtcggtgtat tgcagacaaa tttggcggga caaggcttgg cgaaccttct
     2701 ctaccaagat ggtactatct ttggtgatcg gtctaaagtc cgcaagagtt tccaaaataa
     2761 ccaccatatt acgaatagag acctgttccc gaagcagggc ttgcaagacc ttttgaatct
     2821 cgccaacgga acagagcttg accacttctt ccacgactgc cggataatcc ttgcgtatgg
     2881 tatcgacaat ggcttgtact tcctgtcgac caaggatttc ggaggcatga cgctttatta
     2941 cctcggtaag atgggtagca attattgaag gaggatcgac tacggtatac cccgctctct
     3001 cggctttttc ccggttatcc tcggatatcc aaatggccgg taaaccaaag gctggatcaa
     3061 ccgtcttttc tccgggaagg tcttcgctgg cgccccctgg attgatacca aggaaccagc
     3121 ccatccgtat acgccctctg gctacctcta cacccttaat cttgaagcaa tattcgctag
     3181 gctcaaggcg catattatca ataatgcgaa tgcgcggaac aacgagtcct aaatcaaggg
     3241 cagattcacg gcgaatacgc gttacccgct caaggagttc tgcgccctta tccttgtcta
     3301 caagcgggat aagcccataa ccaagctcca gggagagtgg gtcaagggga actaccgggc
     3361 tgatatccgc gggattctca cctgtttttt gtttttcctt ttcctcttcc cgttcaagtg
     3421 ttttcttgaa tgttcgagcc tcggccttcc tcaatcgcca gccaacccaa gctaatcctg
     3481 cagataccgg aataagaaca taccagggaa aacccggtaa aaaggaaata aacagaagcg
     3541 ttcccgctcc aacataatag acccaggcgt ttcgggagaa ctgtgtttga atatccgaac
     3601 caaaggttcc atccgataca gcccttgtta cgattagacc agttgcaacc gatacaaaca
     3661 gcgaaggcat ttgggacaaa aggccatcgc caatggttaa cccggcatag gtttgaatgg
     3721 caagaccaaa gggctcaccc ctaaagatca taccaaagat aaggccggca acaaggttga
     3781 taaccgtaat aaaaatacca atcttgacgt ttcccgaaac gaatttactc gctccgtcca
     3841 tcgccccgta aaagtccgct tctttttgaa cctctgcctt gcgagcgcgg gactcttctt
     3901 cggtaatggc acccgcattg tattcagctt c
//
LOCUS       DAOQXQ010000065         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24231_length_3865_cov_2.171391, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000065 DAOQXQ010000000
VERSION     DAOQXQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24231_length_3865_cov_2.171391"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKH81_01755"
     CDS             <1..389
                     /locus_tag="PKH81_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96791.1"
                     /translation="YTVAISLYSLILVVSVVVSLFFMQGFFQMLLALIIFSAPLRFII
                     PTFLLVPLLVFSILRIIDTGRWKFFLLLHALNFFVGVLSFLCFISGSLFLSQAGIVLL
                     QAALYMCVWIMALLYTMPSCGQDAQE"
     gene            395..1033
                     /locus_tag="PKH81_01760"
     CDS             395..1033
                     /locus_tag="PKH81_01760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNQ96792.1"
                     /translation="MDITIRLEEPADYRRVEEVQRDAFWDHHAPGCDEHYLAHCMRSH
                     PDFIPELDFVALVDGEIVGNIMYAKAALQNEQGERIPIVSFGPVGTRKSFQRKGVGTA
                     LIRHSLQKARDMGYPMVVIYGYPHDYCKHGFVGSKSLGISDTEGRFPVALLAYDLTGN
                     WKVDGNWKYITSDIYLFDSDAAQAFDATFPPREKGYHTSQEEFRIACQAYVD"
     gene            1263..2135
                     /locus_tag="PKH81_01765"
     CDS             1263..2135
                     /locus_tag="PKH81_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNQ96793.1"
                     /translation="MNTLDYEELDEFEWKVPRKQRQRREISRIKKNAGAEQHVDYFSQ
                     IDFDNVEDYSLFDFFIGEGIIRDKGPVLKQGKEACVYRCPGQGDKASVDVAIKAYKNI
                     EHRSFQSLSSYLQGRLAEAGISRRDAMHILSTPSELQAFWVYSEYSVMERLYQANIPV
                     PVPYAKSATALAMEFIYEESGSSTTAPQLRDVQVNGNTKRIMKEMLLDAIERMLHLNI
                     IHGDLSSYNILVRDNLPVIIDFPQAVDARYNERARPMLERDIRSICRHFPDPAMPPDQ
                     EASLMAAKLWKRYD"
     gene            complement(2140..3582)
                     /locus_tag="PKH81_01770"
     CDS             complement(2140..3582)
                     /locus_tag="PKH81_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96794.1"
                     /translation="MKKNYKRCYYPIAVGLLLFSIFCIRYRTLALSAHPTGLDGYYYA
                     LQAKSFAQTGKLENPDTAPGYYLCGLFAFVSRDAIIGCKVYSALASTLIALSVFTLLC
                     SLCPTQRKKPLLGLLLCAAIPSSVQLSVNYINNQTGLYFFLFYASLIIHLLHARHQRR
                     PTLFALAAVLLILSLLSHLITATYSLFFTACIAYRALPKKITLPLMVGIVLSMLYVAA
                     RHYPRFAGIFSWTPTIPATSSFMRSRLDWRLIAELSLLPILCWAACIYTTIVKKRFIP
                     YIGAAFLLYFPFWGMNSLDMGYRLFLSASPCAIVLLVHLLHNPRNRLTSLTPTYTGAL
                     LMCVAGIVTSSSIYDPQNDPPYEYYRTVANKIDIGDDSLLIAHLGMNHVYTYYQNLRM
                     ALNYVPDFPFRKEKLWRLAYGVQSTTVQHLFPEMDTFEFASLVQPIDRKYLLIREDMW
                     NEYLRREQPDIRETRRNWYNPATPRPKFIR"
BASE COUNT         1040 a          772 c          968 g         1085 t
ORIGIN      
        1 catacaccgt agccatatcg ctctattccc tcattcttgt agtctctgta gtagtttcgt
       61 tattttttat gcagggattt tttcagatgt tacttgcact aattattttt tctgctcctt
      121 tacggtttat cattccgact tttttattag tgcccttgct tgtcttttcg attctccgta
      181 tcatcgatac cggtagatgg aagttttttc ttcttttgca tgcgctcaat ttttttgtgg
      241 gtgtgctttc ttttctttgt tttatttcgg gttctttatt tttgagtcag gctggaattg
      301 tgttgctgca ggctgcgctg tatatgtgtg tgtggattat ggcgcttttg tatacgatgc
      361 catcgtgtgg gcaagacgct caggagtgaa cggtatggat attacaatca ggcttgaaga
      421 gccagcagat tatcggagag tcgaggaagt gcagcgtgac gcgttttggg atcatcacgc
      481 accgggatgc gacgaacact atcttgcgca ttgtatgcga tctcatccag attttattcc
      541 ggaactcgat tttgtcgcac tcgtcgacgg agagattgtt ggtaatatta tgtacgcaaa
      601 ggccgctctt caaaatgaac aaggagaacg catacccatc gtcagttttg gaccggtggg
      661 cacacgaaag agctttcagc gtaagggtgt tggaactgcc ttaatccgtc attctttgca
      721 aaaagcacgc gatatgggat acccgatggt ggtcatttat gggtatcctc atgattattg
      781 taagcatggt tttgtcggat caaagagctt gggaatctcg gatacggagg gaagatttcc
      841 cgttgcctta ttggcatacg atcttaccgg gaattggaaa gttgatggaa attggaagta
      901 tatcacaagc gatatatatc tgtttgattc tgatgcagcc caggcttttg atgcaacttt
      961 tccaccacgg gaaaaggggt atcatacctc tcaagaggag tttcgcattg catgccaggc
     1021 atatgtggat taaggtataa aacctcttgt caaatcccct ttgacagtgc tacagtaaaa
     1081 taaatcaatg ctttgtaagg aggcagtata tgtacggtaa tttggtcata gtgttggcgg
     1141 tactgccttg ttcggttatt tccctctaaa cctataacga taatctgacg gcagtaccct
     1201 tatccatctg ttttttcaca ctcagtaaac agacgtggta tgcccacgcc caaaggatta
     1261 tcatgaacac gttagattac gaagaattag atgaatttga atggaaggtt ccgcgcaaac
     1321 agcgtcaaag acgggagatt tccagaatca aaaaaaacgc cggcgcagag caacatgtag
     1381 attactttag tcaaattgat tttgacaatg ttgaagatta ttcccttttt gattttttta
     1441 tcggagaggg aattattcgt gacaaagggc ctgtgttaaa gcagggcaaa gaggcttgtg
     1501 tgtatcgctg tcctggtcag ggcgacaagg catcggtaga tgtggcgatt aaagcctata
     1561 agaacattga acacaggagt tttcaatctc tttcgtcgta tcttcaaggc aggcttgcgg
     1621 aagcgggaat aagtcggcgt gacgccatgc atattctttc tacccccagc gagttgcagg
     1681 cgttttgggt gtattcagaa tactcggtaa tggagcgctt gtatcaagcg aatattccgg
     1741 tcccagttcc ttacgcaaaa agtgcaacag cccttgcaat ggaatttatc tatgaggaaa
     1801 gtggcagctc tactactgcg ccccagttac gggatgttca ggtgaatggt aatacgaagc
     1861 ggataatgaa ggagatgctt cttgatgcaa ttgaacgtat gttgcacttg aatatcatcc
     1921 atggagatct ctcgtcgtac aacattctgg ttcgggataa tctgccggtt attatcgact
     1981 ttcctcaagc agtagacgcg cgatacaatg agcgagcgcg gcctatgctt gagcgggata
     2041 ttcgatctat ttgccggcat ttccctgatc ctgccatgcc tcctgatcaa gaggcgtcgc
     2101 tcatggctgc caagttgtgg aaacgctatg actaagtgat tacctgataa acttggggcg
     2161 cggagtagca ggattatacc agttacgccg cgtctctctt atatccggct gttcgcgcct
     2221 tagatactca ttccacatgt cctcgcgaat cagaagatat ttcctgtcta tcggttgaac
     2281 aagagaagca aactcaaagg tgtccatttc ggggaaaaga tgttgcactg tagttgattg
     2341 cactccatac gcaagacgcc atagcttttc cttacggaat ggaaaatcgg gaacgtagtt
     2401 aagagccatt cgcaaatttt gatagtatgt gtacacatgg ttcatgccca gatgtgcaat
     2461 taatagtgag tcgtccccga tatctatctt attagcaact gtacggtaat attcataggg
     2521 gggatcattt tgtggatcat agatactgga agaagtgact atgccagcta cacacataag
     2581 taaggctcct gtataagtgg gagttaagga agtaagccgg tttcttggat tgtgtaatag
     2641 gtgtactaaa agtactatgg cgcagggact tgccgaaaga aaaagacgat accccatatc
     2701 aagagagttc attccccaaa agggaaagta taaaagaaag gcggcgccga tgtagggtat
     2761 aaaacgtttc tttactatcg tagtgtatat gcacgctgcc caacaaagta ttggtagaag
     2821 tgaaagttct gcgataagac gccagtcgag tcgggagcgc ataaaggagg acgtcgctgg
     2881 aatggtaggt gtccaggaga atataccggc aaaccgcgga taatgacgag cggcaacata
     2941 gagcatagac aaaacaatgc caaccataag gggcaaggtt atcttttttg gtaatgcgcg
     3001 gtaagcgata caagctgtaa aaaacagact ataggtagca gttattaaat gagataaaag
     3061 agagagaata agcagtactg ctgcaagtgc aaagagggtg gggcgtcttt ggtgtcttgc
     3121 atgaagcaaa tgaataataa gcgatgcgta aaataaaaag aagtaaagac ctgtctgatt
     3181 gtttatgtaa ttaacagaga gctgtaccga agatgggata gctgcgcaca agagaagccc
     3241 gagtaagggt ttctttcttt gtgtcgggca gagactacaa agaagggtga acactgataa
     3301 ggcgatgagc gtggatgcaa gggctgaata aaccttgcat ccaataattg cgtccctgct
     3361 tacaaacgcg aatagtccgc atagataata tcccggagct gtatcaggat tttccagctt
     3421 accggtttgc gcaaaagact ttgcctgaag cgcatagtaa tatccatcaa gcccggttgg
     3481 gtgcgctgat aaggcaagtg tcctgtaccg tatacaaaag atactgaaca gcagtagccc
     3541 cacggcgatt ggatagtagc agcgcttata gttttttttc atactttgaa cttaccacag
     3601 aaaatcccag ggaagtgatg tgtttttgag ctgccttgtt aaatgtccat atatgacata
     3661 ccgcactggt agctcccgca ttctttgccc gactaagggc ggtttgaaca agattcttgc
     3721 ttgtcctgcc ggatctgaaa cccggatctt tgtcacgggg gatgaaatcg tctgggaccg
     3781 ccggcgtcaa ggtcatcgac ggctaccggc tcatgttctt cgcctttggc ctggaagcgc
     3841 tgaactcacg ccagcagcgg attga
//
LOCUS       DAOQXQ010000066         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24358_length_3850_cov_3.186034, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000066 DAOQXQ010000000
VERSION     DAOQXQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24358_length_3850_cov_3.186034"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2298)
                     /locus_tag="PKH81_01775"
     CDS             complement(<1..2298)
                     /locus_tag="PKH81_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96795.1"
                     /translation="MIHHRILRVCILFLLLLPLSGRDFYWESPESVASSDSRFPSSAT
                     NGTVSAVVWHDVEAKDKDSGLIWLSGRVYDGSRWYDRPRFAGPFSYAGEVPSIASVTI
                     DRDNRILVPVASAFSDIAVMVSSDLGSSFNTYHLDGRGQGQLAPRIFVRSDGGYFLFA
                     ARGTEDNFIMVFSRSDNATEWQPLAPFGPASGRKRAFIPSHASDGSNDVVVFQAFHEG
                     ERRSSYQLYSTVSRDKGSSWSDAALITNFGENPSIQDEAVSGFTNYHNQRARLLKING
                     TISLVWERSRTMTEKYAIWHASLNAQGSIVGNAELVSSTDGYCYDPDLISLDQQPVVV
                     WFDNRRGVNRVYMARKEGFLWTETDLSRGPVDSVFARIVPTAESLEVYWQENRPRGVF
                     RIARLAPDHSVQPPKLIAVNFAAGKKSRGDIVRFSVALPEDSSGIAGYSLESSAFRKP
                     DVPAEIQYLPSDVARSFSVDYDASWIIGVRAVDYAGNWSEPAYLEYIRDTTPPLPPTI
                     VERQKDSNGYLVSNDVTLEWTVAEPDDIAGFTWNFEYVASLDYLPRLAARSSASTSQI
                     PISRSAPALFDFQGEAAKVFTPKKPDATVRSATPSVSFANQDNGIYALSVSTVDSVGN
                     ISRPSIRYYALNKYIPVTYITYVDAQTDESGSVSLSLVGRGFSHGGPLDEILIDRDGV
                     EPWDLRLRLQSGQYKIVNDRLVEGITLRDLDEGAYRVIVSHPLRGRHASRNLLSVSES
                     GTVKFGDFRHVFTPPWTIAEPLSKRS"
     gene            complement(2295..>3850)
                     /locus_tag="PKH81_01780"
     CDS             complement(2295..>3850)
                     /locus_tag="PKH81_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96796.1"
                     /translation="LDGIRLSLYRGIAANQDASSSLLAQEWIERHDEANRLLDGVTDE
                     DSSTVRYYPAESLALFTEVRRGVTADIAVIQRLQSAMTASPPAILADQDYASRLASVD
                     AVRSRLEALANDASAGITRANSRVLQANLAKQESDLRFRQAQDALRRSDFQLARDNLQ
                     RSRERINQSLSIQESASIRTETDIRLSNLGAEITRIENESVVREVRALISSGKNLYYL
                     GNFDQAEQLLIQARNRWGVTNIEPNAEVNNWLDIINTALSMKTGRTIPVSAPLYPQMS
                     QILSSANQLYSEGRTLMSVGKRTEAISALTSARSQLQQLQLVYPLNQDAGTLSLKIDQ
                     VIDPDAFKEFFSQKVDYIRANYRSERQTAYSDLLDLYLINPGYPGLSRLVEEVEIYLG
                     IRLPPPDPRSLSRSRELTQSAQRIYDANTRSMFQVALNQLDEAIKLNPENQSAIVLKD
                     RVQTAIGGGAVAVLSAEDEAKYQQAVQELQKGNKITASALVEQLLQNPKSRNATKIID
                     LKKRIDSQL"
BASE COUNT         1089 a          934 c          920 g          907 t
ORIGIN      
        1 ggatcgcttt gataacggct ctgctatcgt ccagggaggg gtaaacacat gacggaaatc
       61 cccgaacttg accgttccgg attcactaac cgaaagcaga tttcgcgaag catgacgacc
      121 gcgaagagga tgggaaacaa taacgcggta agcgccttca tccaaatccc tgagggtaat
      181 tccttcaacc agacggtcat tgactatctt gtactgacca ctttgcaagc gtaatctaag
      241 gtcccacggt tccacaccgt ctcggtcgat caatatttca tcaaggggtc ctccatggga
      301 aaaacctcga ccgaccaatg ataaagaaac agatccggac tcgtcagttt gtgcatctac
      361 ataggttata taggtaaccg gaatatattt attaagtgca taatagcgaa ttgagggtct
      421 acttatgtta ccaacagaat cgacagtgga aacagacaag gcataaattc cattatcctg
      481 atttgcaaag gatacagaag gagttgccga gcgaacggta gcgtcaggtt tttttggggt
      541 aaacaccttt gctgcttcac cctggaagtc gaataaggcc ggcgcagagc gggaaattgg
      601 tatctgactt gtagatgcag aagaacgtgc ggcaagacgc ggaaggtaat caagagaggc
      661 cacatattca aaattccagg taaaaccggc aatatcatct ggctctgcga cagtccattc
      721 aagggtgaca tcattggaaa ccagatatcc attactatct ttttgacgct caacaattgt
      781 gggcggtagc ggtggagttg tatcgcgaat gtattcaaga taggcaggtt cagaccagtt
      841 acccgcataa tcaaccgcac gcaccccgat aatccaggac gcgtcatagt ccaccgaaaa
      901 actccgagct acatcagaag gaagatattg aatctccgca ggaacatccg gttttctgaa
      961 tgcagatgat tcgagcgaat aaccggcaat accggaggaa tcctctggaa gggcaacact
     1021 aaaacgaaca atatcgcccc tgctcttttt tccagcagca aagttaaccg cgataagttt
     1081 tggcggttgc actgaatgat cgggggcaag tcgagcgatc ctgaacaccc ctctcgggcg
     1141 gttttcttgc caatacacct caagactttc tgcagtaggg actatgcgag caaatactga
     1201 gtcaactgga ccccgcgata aatctgtctc cgtccataaa aagccttcct tgcgagccat
     1261 gtagacacga ttaacaccac ggcgattgtc gaaccataca acaacaggct gctgatccag
     1321 cgatatcaaa tcaggatcat aacagtaccc atcggttgag gaaacgagtt cagcattccc
     1381 aacgattgat ccctgagcat taagagatgc atgccagatg gcatacttct cggtcatagt
     1441 acgagagcgc tcccagacta atgagatcgt tccatttatt ttaagtaacc gggctcgttg
     1501 attgtgataa ttagtaaaac cacttaccgc ctcatcctga atcgaaggat tctccccaaa
     1561 attcgttata agcgcagcat cgctccagga cgatccctta tcgcgtgata cggttgaata
     1621 caactgatac gacgatcgtc gctcaccttc gtgaaaagcc tgaaagacca ctacgtcgtt
     1681 agatccatca gaggcatgag aggggataaa cgcgcgctta cgacctgatg caggaccaaa
     1741 gggagcaagc ggttgccatt ccgttgcatt atccgagcgg gagaacacca ttataaaatt
     1801 atcctcggta cctcgtgccg caaagagaaa ataacctccg tccgagcgaa caaaaattcg
     1861 cggagctaat tgcccctgcc ctctgccatc aagatgatag gtgttgaatg aggagcctag
     1921 atcggatgag accattaccg cgatatcgct aaaggcactt gcgactggaa caagaatacg
     1981 gttatcccga tcgatagtta ctgatgcaat tgaggggact tcccctgcgt acgaaaatgg
     2041 acctgcaaaa cgagggcgat cataccagcg actcccatca tacacgcgac cggaaagcca
     2101 gatgagccct gaatccttgt ccttagcctc gacatcatgc catacaactg cagatacagt
     2161 accattggtc gctgatgagg ggaaacgact gtctgacgaa gcaacggatt ccgggctttc
     2221 ccagtaaaaa tccctgccgg aaagaggaag caaaaggaga aaaagaatac aaacgcgaag
     2281 gatgcggtga tgaatcataa ttgcgaatct atccttttct taagatcaat aattttagtt
     2341 gcgtttcggc ttttcggatt ttggagtaac tgttcaacaa gagcagacgc tgtaatcttg
     2401 tttccttttt gaagttcctg tactgcctgc tgatatttgg cctcatcttc ggcagataga
     2461 actgccaccg ctccgcctcc gattgctgtt tgtacacggt ccttaagcac aatcgcgctt
     2521 tgattttccg gattcagttt aatcgcttca tcaagctgat taagagcgac ctggaacata
     2581 ctacgggtat ttgcatcata aatcctttgc gccgattgtg taagctcccg cgagcgcgaa
     2641 agggatcgtg gatcaggagg aggaagtcgt atacccagat atatttcaac ctcttccaca
     2701 agacgagaaa gccccggata gcctggattg atcaaataca gatcgagcag atcgctatag
     2761 gcagtttgac gttcactgcg atagttcgca cgaatatagt ctaccttctg actaaagaac
     2821 tctttaaaag catcaggatc aattacctga tcaattttta gagacaaggt accggcatcc
     2881 tgattaagag gatacaccag ttgaagttgt tgtaattgcg accgcgccga ggttaaagcc
     2941 gatattgcct cagtcctttt accaaccgac ataagggttc ggccctcgct atacagctga
     3001 ttcgcgctgg aaagaatctg gctcatctgt ggatacaagg gtgcggatac aggaatcgtg
     3061 cgaccggttt tcatggataa agcggtgtta atgatatcga gccagttatt cacttccgcg
     3121 ttgggctcaa tattggtaac cccccaacgg ttgcgagctt gaatcagaag ctgttcagcc
     3181 tgatcaaaat ttcccaagta atacaaattc tttcccgatg aaatgagagc gcgaacctcg
     3241 cgaacgaccg actcattttc tatgcgagta atctccgctc caaggttgga aagacgaata
     3301 tcggtctctg tacgtatcga agccgattcc tgtatagaca aagactgatt tatccgttcc
     3361 cgcgaacgtt gaagattatc ccgagcaagc tggaagtcgc ttcgcctgag cgcgtcttgg
     3421 gcctgacgga aacgcaggtc tgattcttgt tttgcaagat tcgcctgaag aacccgtgaa
     3481 ttggccctgg taatacctgc agaggcatca ttggcaagag cctcaagtcg ggagcgcact
     3541 gcatcaactg aagcaagccg agaagcatag tcttgatcgg caagaattgc cggtggagat
     3601 gcggtcatgg cagattgaag gcgctgaata acggctatat ctgcggtaac tccacgacgc
     3661 acctcggtaa agagggcaag gctttctgcc ggataatagc ggacagtgga ggaatcttcg
     3721 tccgttaccc catcaagcag ccgatttgct tcgtcatgac gctctatcca ttcctgggca
     3781 agcaaactgg atgatgcatc ctgattggca gcaatccccc tatacaagga aaggcgaata
     3841 ccatcaaggg
//
LOCUS       DAOQXQ010000067         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24442_length_3842_cov_3.012147, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000067 DAOQXQ010000000
VERSION     DAOQXQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24442_length_3842_cov_3.012147"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKH81_01785"
     CDS             complement(<1..707)
                     /locus_tag="PKH81_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNQ96797.1"
                     /translation="MSSMDLVEGKVTRISGPIVYAEGLGSCGLYDVVDVGEAALIGEI
                     IRLKNGIATIQVYEDDTGMHVGEKAVCLRRPLSVRLGPGLVGTIYDGIQRPLESLYKS
                     SGAFLNPGDRSESLDIQKKWHFSPTTMDDGTLLSAGNSISSGFTLGTVQESASVTHKI
                     MVPPTIRGRSLSWFAGEGEYTVNDVIAKTELDEPILLSHYWPVRKPRPFSGKLSVSEP
                     LITGQRVIDVFFPLSKGG"
     gene            complement(704..1330)
                     /locus_tag="PKH81_01790"
     CDS             complement(704..1330)
                     /locus_tag="PKH81_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96798.1"
                     /translation="MEELRSTEILDREIQEDARRKAEKILKSNEAECRQILEEVDRRI
                     AALRTDKGKEYAGRLEVYKRDVQASIPLEKQRRLVSFIDNEVNQALDAWFTSIGESRR
                     LLVYRQLLLRFKKSLNGKKMMVKVSGYTQEEARKLVESVFSPEAIDSVTVLDAPGKAA
                     LSLQDGFYLEDLGKTLSCRAGEQEVKNELLSTRREELAKALMGGRIPQ"
     gene            complement(1351..1692)
                     /locus_tag="PKH81_01795"
     CDS             complement(1351..1692)
                     /locus_tag="PKH81_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNQ96799.1"
                     /translation="MKYYIIGERELVLGFSLVGVTGTVARTREEALDAFYRVTGRGNL
                     LAANVPVQEERPRVLILTEEMTALLEEEVREWQMAGDYPLIVEIPGLHGHLSGRKTLT
                     DSIREAIGIHV"
     gene            complement(1712..2032)
                     /locus_tag="PKH81_01800"
     CDS             complement(1712..2032)
                     /locus_tag="PKH81_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNQ96800.1"
                     /translation="MNRKLLTTISLTLACAALGFAEAHAPAAEATQSAGLSIAAFKYI
                     AAAIAVGIACIAGGMAVGRIGAAAMGAMSENAELSGKALPFVGLAEGICLWGFLVALL
                     IILL"
     gene            complement(2034..>3842)
                     /locus_tag="PKH81_01805"
     CDS             complement(2034..>3842)
                     /locus_tag="PKH81_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNQ96801.1"
                     /translation="ARAYLGVADAQALSTGTTLPSEEDRQGADAILAEVEDIRKREIE
                     ASDLRKRIGSAYDEARAFANLKVAYSELEHLTFLSMRIGKIDPAVLDELQFAIGDRAV
                     ILPLGEDRTRILAASSKKGRFALDTELKRFGFIPLDVPEDFKGVPEEVLIGLQNQTQG
                     ASGALEAVAAERMRYAETHTQEIQRLLETFSLGAQVQDVRESLEATQLVYRIMGWIGL
                     DESATLMKDLDNLTEGRIAMRMYAPNEVPSVKSGREKVPVQYTHGKFVKSFERMIFSY
                     GAPLYGTIDPTPVVAFFFTLLFGIMFGDAGQGLVFLLLGLLLSYQKMPPLRKFYHFGP
                     IFMAVGCSSVLMGLLTGEFFANSELLKPFSRFVTGLFGESRDHILHMMPSKDSLDKLM
                     IFFGFTLAVGFMINSVGLIINIINQFSLSRPAKAIFSKTGICGALFFWYVVFAAIRIA
                     VTASSVQWYDFVCVGIPLFGVFFAEPFSRLIEGHRPVLENGLFSAIIEGVVEILEIVS
                     TYISNSVSFLRVGAFALSHAVLSFIIFTMSELVGGSLSAGGLVIGVFGNLVIIVLEGL
                     IVTIQVIRLQYYEFFSKFFTETGREFKPFRFTYKEQ"
BASE COUNT         1082 a          998 c          847 g          915 t
ORIGIN      
        1 ccacctttag ataaggggaa aaacacatca attacccgtt ggccagtaat aagaggctca
       61 gagacagaga gcttaccgga aaaagggcgg ggtttgcgaa ccggccagta atgagaaagc
      121 aagataggct cgtccaattc ggtttttgcg ataacgtcgt taacggtgta ttccccttct
      181 ccggcaaacc aggaaagaga tcgaccccga attgttggcg gtaccattat cttatgagtg
      241 accgacgcag attcctgaac cgttcctaag gtaaagccac tgctaatcga gtttccagcg
      301 cttagcaaag tgccatcatc catcgttgtg gggctaaagt gccatttttt ttgaatatcc
      361 aagctttcgc ttcgatctcc gggatttaaa aaggctccgg aagacttgta taaactttcg
      421 aggggacgct gaattccgtc gtagatagtc cccaccaaac cagggcccaa acgaacagat
      481 agaggcctgc gcaaacacac tgccttttcg ccaacgtgca taccggtgtc atcttcgtac
      541 acctgtatcg tagcaatccc gtttttaagg cggataattt caccgatcag ggccgcctct
      601 ccgacgtcta cgacgtcata gaggccgcac gacccgagac cttccgcgta cactatcggt
      661 ccgctaatgc gcgtaacctt tccttctact aaatccatgg aactcactgc ggtatcctcc
      721 cgcccatcag agcctttgcc aattcttcac gtcgggtgga caataattca tttttaacct
      781 cttgctcgcc tgcgcgacaa gagagggttt tgccaaggtc ttcaagataa aaaccgtctt
      841 gtaacgaaag agcggcttta ccgggagcat cgagtacagt aactgaatcg atagcctcgg
      901 gagaaaaaac cgattcaaca agttttctcg cttcttcttg tgtatagcct gagaccttaa
      961 ccatcatttt ttttccatta agggattttt taaagcgaag caataactga cggtatacca
     1021 aaagcctacg actctcgcct atggaagtaa accatgcatc aagagcctga ttgacttcgt
     1081 tgtcgataaa ggatacaagg cggcgttgct tttccaaagg gatagaggcc tggacatccc
     1141 gcttatatac ctcaagacgt ccggcatatt ctttgccctt atcggtgcgc aaagccgcaa
     1201 ttcgccgatc aacttcctca agtatctggc ggcattctgc ttcgttcgac ttgagtatct
     1261 tttctgcttt acgccgggca tcctcctgga tctcccgatc aagtatttcc gtagaacgta
     1321 attcttccat tgcttcctta acccgctttc ttatacatgt ataccaatcg cttcccgaat
     1381 agaatcggta agtgtttttc tgccgctcaa gtgtccatga agtccgggta tctcgacgat
     1441 aagaggataa tctccggcca tttgccattc ccttacttct tcctcgagca aggcagtcat
     1501 ctcttcggta agaataagca ctcgtggacg ctcttcctga acgggaacat tggcagcaag
     1561 aagattcccc ctgccagtta cccgataaaa agcatcaagt gcctcttccc gagttcttgc
     1621 taccgtaccg gtaacgccaa ccaaagagaa gccgagaact aactctcttt ccccgataat
     1681 gtaatacttc acgaagcgta acctgcgtct gttacaacag gatgatcagc agggcgacaa
     1741 gaaagcccca cagacagata ccttccgcca agccaacgaa cggaagggcc ttaccggaaa
     1801 gctcggcgtt ctcgctcatt gcgcccatag cggccgcacc gatgcgacct accgccatgc
     1861 caccggcgat acaggcgata cctaccgcga ttgccgcggc gatatactta aatgcggcga
     1921 tggaaagccc tgccgactgg gttgcttcgg cagcgggggc gtgagcttct gcaaagccaa
     1981 gggctgcaca ggccagggta agagaaatgg tggtgagtaa cttgcgattc atattattgc
     2041 tccttgtatg tgaatctgaa gggtttgaat tcccttccgg tttctgtaaa aaactttgag
     2101 aaaaactcgt aatactgaag acggataacc tgtatagtta ctatcaatcc ttcaagcacg
     2161 ataatgacca aattgccaaa tacgccaata accaatccgc cggcggaaag ggatcctccg
     2221 acaagctcgg acatggtaaa gataataaag ctaagaaccg catgagagag ggcaaaggcc
     2281 cctactcgga ggaagctgac ggaattagat atgtaggtgg acacaatttc aaggatctct
     2341 acgacacctt cgattatcgc ggaaaaaagg ccgttttcca ataccggacg atgaccttcg
     2401 atgagcctgg aaaatggttc tgcaaagaaa actccaaaaa gaggaatacc cacgcagaca
     2461 aagtcatacc actgaacaga agaagcagta accgcgatgc gaatagcggc aaacactaca
     2521 taccagaaaa acagggcccc acaaataccg gttttactaa aaatcgcctt ggcaggacga
     2581 gaaagagaga actgattgat gatattgatg ataaggccta ccgagttgat cataaaacca
     2641 accgcaaggg taaagccaaa aaagatcatc agtttatcaa gcgaatcctt tgatggcatc
     2701 atgtgaagaa tatgatcccg gctttctcca aacagtccgg taacaaaacg gctaaacggt
     2761 ttaagaagtt ccgagttagc aaagaactcg cccgttaaaa gtcccattag tacacttgaa
     2821 cagccaacag ccataaagat tggcccaaag tgatagaact tgcgcagggg tggcatcttt
     2881 tgatagctca agagcaagcc aagcagcaaa aaaaccaaac cctgacccgc atctccaaac
     2941 attatgccaa aaaggagagt aaagaaaaaa gcgactaccg gggttggatc tatagtgcca
     3001 tataacggag cgccgtaact aaaaatcatc cgttcaaaac ttttaacgaa ttttccgtga
     3061 gtatactgaa caggaacttt ctcccttccg ctttttaccg agggaacttc gtttggcgca
     3121 tacatcctca tggctattct gccttcggtc aaattatcca aatccttcat aagagtcgcg
     3181 ctttcatcca ggccaatcca tcccatgatt ctatatacca gctgtgttgc ctcaagactc
     3241 tctcgaacat cctgtacctg cgctcccaag gagaaggtct caagcaaacg ctgaatttcc
     3301 tgagtatgag tctctgcgta cctcattctc tcggcagcaa ccgcttccaa ggctccagag
     3361 gcaccctggg tttgattttg caaaccaatc aagacctctt ccgggacacc cttaaaatct
     3421 tccggaacgt cgagcggtat aaagccgaat ctctttaatt cagtatcaag ggcaaatctg
     3481 cctttttttg aagaagcagc caatattcgg gtacggtctt cgccaagggg cagaataacg
     3541 gcgcggtccc caattgcgaa ttgcaattca tccaggaccg caggatcaat cttaccaatt
     3601 ctcatggaca aaaaagtaag atgctcaagt tcactgtatg caacctttaa attggcaaag
     3661 gcgcgtgcct catcataggc actcccgatt cgcttgcgta aatcggatgc ttcaatttcc
     3721 cgcttacgaa tatcttcaac ttcggcaagt attgcatcag cgccctgccg atcttcttcc
     3781 gatggaaggg ttgtaccggt tgataaggcc tgtgcatcgg ctaccccaag atacgctcgg
     3841 gc
//
LOCUS       DAOQXQ010000068         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24510_length_3834_cov_3.002117, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000068 DAOQXQ010000000
VERSION     DAOQXQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24510_length_3834_cov_3.002117"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKH81_01810"
     CDS             complement(<1..789)
                     /locus_tag="PKH81_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNQ96802.1"
                     /translation="MPSQITHVLGGWRSRNDASPDPAFALGCQGPDVFAHGRRTKPFA
                     LSYARLLHRRGYGDFCVSCARLLRDTQDPVLLSWFSGFLTHQALDKALHPYIVCRTPS
                     WSGTNQDNQVLRGVSPALYHAFFERILDVLLYELYEQHPVVHFNARKVFHADPAAIDC
                     IVALISRCLRETYPDQSATDADLDERIQNAFADTYYFYALTDPSRTNAPSHELPRSIA
                     AYASRGASGVALLFPVALDTSIDYLNLTSSPWPDPVSGLSRRESV"
     gene            complement(795..1544)
                     /locus_tag="PKH81_01815"
     CDS             complement(795..1544)
                     /locus_tag="PKH81_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ96803.1"
                     /translation="MSDTSDIPDLSSDARTKVLIAEDDTIVALDLQGMITRLGYDVVA
                     IVDNGQAAVAAARRFLPDVILLDMAIGGGIDGIDAAREINKSSSTPVVFCVSSPDLAL
                     LARAKEISYAGYLLKPINPDSLATTLDTVLYKYKLEKRAELAEERYRILSDNCEVLKN
                     FMDAGLTHSITWKADTGLVYTSDGSSTDVSFFEPIREQLISLLSRVTNDDRLATVLKP
                     SSCDNQFMILGVRNPDSQAVSALVIPLSVSE"
     gene            1693..1766
                     /locus_tag="PKH81_01820"
     tRNA            1693..1766
                     /locus_tag="PKH81_01820"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1727..1729,aa:Pro,seq:ggg)
     gene            complement(1834..2550)
                     /locus_tag="PKH81_01825"
     CDS             complement(1834..2550)
                     /locus_tag="PKH81_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ96804.1"
                     /translation="MKIAFISDIHGNIHAFESVLKDLKKQKADRIIFLGDASTLGIRP
                     VESLELLQSLNCDCIMGNHDEFMIDPAKIYAYTKDKLVIDTVEWSRARHSKRHFNFIK
                     TFKKDLRVPFLPDTDIYCYHGSPTSNMENVKPESPVDSFTPLIREKSNTIFIGGHTHV
                     QMLRNYAGTRIITSGSVGQPFIGYTMPPVKHPWAEYVLIEGRGKHYNITFRQVEIDND
                     KINKEILASDSPMNDFISEF"
     gene            complement(2575..>3834)
                     /locus_tag="PKH81_01830"
     CDS             complement(2575..>3834)
                     /locus_tag="PKH81_01830"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNQ96805.1"
                     /translation="NRSLLLDRLSQRLAFIQRYPRKDVLVLLNLDRFKVINDARGNQL
                     GDSLLTAVATRLQSIMREADTVARMSADEFALLLPSDDSNSEQIGLTTMIITERIRDA
                     LRSPFLIEDESFVVTASFGVTLLPITDDDTPQLALKRADTALHRAKDAGGNQYAFFDS
                     EMGETASENYRVERELADAIRDGQLRLFLQPQVDSSGSIVAAEALVRWEHPSRGLLSP
                     AAFIPIAEQSDLIIEVGKWVLTQACGIIAGCDKAGQMVHISVNMSPRHFRKANFTLWV
                     KNLLERSGAKPNCLTLEITEGLFIGDMADIVAKMNELVDLGISFSLDDFGTGYSSLAY
                     LKRLPIHELKIDKSFVQDAPTDTNDAALVEAILAVASLLKLRVVAEGVETHLQAEFLN
                     ARAHVLHQGYLYGKPESAEKIIERWKM"
BASE COUNT         1082 a          906 c          879 g          967 t
ORIGIN      
        1 aacgctttcc cttcttgaca aaccggacac tggatcaggc cacggtgagg aagtgagatt
       61 gagataatcg atggaggtat ccagggccac cgggaacaat aaggcaaccc cggaagcccc
      121 acgcgaggca taggcggcaa tagatcgggg aagttcatga gacggagcat ttgttcttga
      181 agggtcggtt aaagcataaa aataataggt gtctgcaaag gcattttgaa ttctctcatc
      241 caggtctgca tcagtagcac tctggtccgg gtatgtttcc cgtaaacagc ggctaatcaa
      301 tgccacaata cagtcaatcg cagcggggtc ggcgtgaaac accttgcggg cattaaaatg
      361 gacaacaggg tgttgttcat acaattcata gagtaataca tccaggatac gttcaaaaaa
      421 agcgtgatac aaagcaggag atacaccgcg caacacctgg ttgtcctggt ttgttcccga
      481 ccatgaaggc gttcggcaca caatataggg atgcaaggcc ttgtccaggg cttgatgagt
      541 caaaaatccg gaaaaccagg aaagaaggac agggtcttgg gtatctctaa gcaggcgggc
      601 acacgagaca cagaaatctc catagcccct gcgatgcaga agccgtgcat acgacagtgc
      661 aaagggtttg gtcctccggc catgggcaaa aacatccgga ccctgacacc ccaaggcgaa
      721 ggctggatct ggcgaagcat cattgcgtga acgccaccct ccaaggacat gagttatttg
      781 ggaaggcata ctcgttactc ggaaacagac agcggaatga ccaaagccga aaccgcctgg
      841 gaatcaggat ttctgacgcc aagaatcata aactggttat cacaactaga aggctttaac
      901 acagttgcaa gccgatcatc gttagtaacc cgggacaaca aggagatcaa ctgttcccga
      961 attggttcaa aaaaagagac atcagtggat gatccgtcgc tagtgtatac aagaccggta
     1021 tctgctttcc atgttattga atgagtcagt cctgcgtcca taaaattctt aagaacttcg
     1081 caattgtctg ataatatacg ataacgctct tcggcaagtt cagcccgctt ttcaagcttg
     1141 tatttgtaca agacggtatc aagcgttgtc gcaaggctgt ccggattgat gggttttaat
     1201 aaatatccgg cataggatat ttcttttgca cgagcgagca aggcaagatc gggacttgat
     1261 acacaaaaca ctacgggcgt tgaggagctc ttgttaatct cacgggcggc atcaatgccg
     1321 tcaataccgc cacctatagc catatcaaga agaataacgt ccggtaagaa ccgacgagcg
     1381 gcggcaacag cggcctgacc attatcaacg atagccacga catcatagcc aagccgagtt
     1441 atcatcccct gtaaatccag ggcgacaata gtatcatcct cggctataag aaccttagtc
     1501 ctggcatccg aggaaagatc aggaatatcg cttgtgtcac tcataaaccg gtcactcaca
     1561 gctaaagtct atagtgtttt agtcaatcct tcaagccgga tttctccgga cgaggctctc
     1621 ttgacactcc ccccccgtta tggtattttt tccctcgcct ttcctgcagt caattctgtg
     1681 agtgtcttta gtcgggcaat agcgcagttg gttagcgtac aagtctgggg gacttggggt
     1741 ccccggttca aatccgggtt gcccgactaa agacactggt tattaaaaaa aaatggcggc
     1801 tattcgccgc cacataagct taccgatact gtcttaaaac tcactgataa aatcgttcat
     1861 cggactatca cttgccaaaa tctctttatt aatcttgtcg ttatcaatct ctacctgtct
     1921 gaacgtaatg ttatagtgct taccccgccc ttcgatcagt acatactccg cccagggatg
     1981 ctttacggga ggcatggtat agccgataaa aggctgtcct acactccccg aagtgataat
     2041 acgggttccg gcatagtttc gcagcatttg aacatgggta tgccccccga taaaaatggt
     2101 attcgatttt tcccgaatta acggggtaaa actgtccacg gggctttcgg gtttaacatt
     2161 ttccatattt gatgtaggag agccatgata gcaataaatg tcggtatcgg gaagaaaagg
     2221 tacgcgaaga tcctttttaa aggtctttat aaaattaaaa tgtcgctttg aatgacgagc
     2281 tctggaccat tctaccgtat caataaccag tttatccttg gtatatgcat aaatctttgc
     2341 cggatcgatc atgaactcgt catggtttcc catgatacag tcgcaattaa gggattgcag
     2401 gagttccagg gattcaaccg ggcgaatacc cagagtcgat gcatcaccca aaaaaataat
     2461 gcgatcggct ttttgttttt ttaggtcttt tagtactgat tcaaaggcat ggatatttcc
     2521 gtgaatatct gaaataaaag ctattttcat agtgatgagt atacacccgc ttttttacat
     2581 tttccacctt tcaattattt tttctgcact ctccggtttt ccatataaat aaccctgatg
     2641 aagaacgtgc gcgcgagcgt ttaaaaattc agcttgcaag tgcgtttcga ctccctctgc
     2701 aactacacgg agctttaaca gactggcaac agcaagaatt gcctcaacca gggccgcatc
     2761 attagtgtct gtcggggcat cctggacaaa ggatttgtct attttaagtt catgaatcgg
     2821 taagcgtttt agatacgcca aagatgaata gccggtgcca aaatcatcaa gggaaaatga
     2881 tatgcccaag tctaccaatt cattcatttt tgcgacaata tcggccatat ctccgataaa
     2941 aagtccttcg gtaatctcca gggttaaaca atttggcttt gctccacttc gttccaacag
     3001 gttcttgacc cataaggtaa aatttgcctt tctaaagtgc cgcggactca tatttaccga
     3061 aatatgtacc atttggccgg ccttatcgca gccagcaata atcccgcagg cctgtgttaa
     3121 tacccatttt cccacttcga taatcaaatc cgattgctct gcaatcggaa taaaggcggc
     3181 aggtgacagc aagcctcgtg acgggtgttc ccagcggacc agggcttccg cagcaacaat
     3241 cgaacccgaa gagtcaacct gaggctgtaa aaacagacgt aattgcccat ctcgaatcgc
     3301 atccgccaac tctcgttcaa cccgatagtt ctctgaggca gtttcaccca tttccgaatc
     3361 aaaaaatgca tattgattgc cgccagcatc tttggcacgg tgcaaggcgg tatcggctct
     3421 ttttaacgcc agttgtgggg tatcatcatc ggttatcggt aataaggtaa caccaaagct
     3481 cgcggttacc acaaaggatt catcctcaat caaaaaggga cttcgtaatg cgtcccggat
     3541 tcgttcggtg atgatcatgg tagttaaacc tatttgctcg gaattggaat catcagaggg
     3601 gagaagcagg gcaaactcat ctgcgctcat acgagcgacg gtgtcagcct ctctcatgat
     3661 cgattgaaga cgggttgcga ccgcagttaa aagactatcg cctaattgat taccccgagc
     3721 gtcattaata accttaaaac ggtcaagatt gagtaaaaca agtacatctt tgcggggata
     3781 ccgttgaata aaggcaagac gttgcgacag ccggtcaaga agaagactgc gatt
//
LOCUS       DAOQXQ010000069         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24692_length_3816_cov_3.496676, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000069 DAOQXQ010000000
VERSION     DAOQXQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24692_length_3816_cov_3.496676"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKH81_01835"
     CDS             <1..429
                     /locus_tag="PKH81_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96806.1"
                     /translation="TIEVAFNLTMVENLFVELGFAMPTDTDVSGEWKKISMYGNYKMD
                     AMTFHLIAILKMLKDDNQMNFGAGMDYALDGGIGINADVRYYNDIWNATVDNPDPDAR
                     ITFMVGATKGFSNGLIGAGLEVVSADETGYMVPVRMEYWF"
     gene            538..1209
                     /locus_tag="PKH81_01840"
     CDS             538..1209
                     /locus_tag="PKH81_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96807.1"
                     /translation="MMSKDKVLYALVASFFLLASCASKVDTSGEPDVIAMVDPFQVAT
                     LGMGLSDLLGLGMNQTTFDLFFAPRGNATIMAFSYQMNKTEVFLDYTARQTVIAAVGT
                     YLEEYEAKTLRTKGKTTAEYGKMQGYMEWGVLTKNARATPEISIGYDFVKDSPYFTLT
                     VPESTNLIYEGVGGSRVQRSAYMRFYFNREQAVEFAQHLSQEFLLEALGEQDVPEASF
                     TPDSY"
     gene            complement(1269..1688)
                     /locus_tag="PKH81_01845"
     CDS             complement(1269..1688)
                     /locus_tag="PKH81_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNQ96808.1"
                     /translation="MRVFIDTNILISSILFPEGKTAVVFNHIIEHHELIIASFCIAEA
                     TMVFSRKFPDKCPLLEQFFLDLNFELFHTPESVSLTEYPQIRDPCDYPVLVSAILSDS
                     DILLTGDKDFDGVEITKPLIFNPSQYFELITKNEQGK"
     gene            complement(1691..1942)
                     /locus_tag="PKH81_01850"
     CDS             complement(1691..1942)
                     /locus_tag="PKH81_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ96809.1"
                     /translation="MELAKVTSKGQITIPQEIRKKLKLKEGDKVFFVEDNGSLQFLNA
                     SQVAIRAVQNGMSGEAKKAGFTTEDDVVQYIKRIRNTAT"
     gene            complement(2100..2381)
                     /locus_tag="PKH81_01855"
     CDS             complement(2100..2381)
                     /locus_tag="PKH81_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ96810.1"
                     /translation="MSEVLAAILFTIQGRVQGVGFRAWAAQDARRLGLTGWVRNASDG
                     SVELYAEGPVNALEELYARLRQGNGLSTTVSIQQKHVTPQGATRFDITC"
     gene            complement(2378..3463)
                     /locus_tag="PKH81_01860"
     CDS             complement(2378..3463)
                     /locus_tag="PKH81_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ96811.1"
                     /translation="MAFNLSMYPVSYIAQYNPATNTWDESWYQADSITKDDLDAMSEA
                     EQSKVYAKRNTFNLPLINYTTQYAMGCFEGMKAYPQKDGSLSLFRPDRNAARFNASMK
                     GLYAPAFPQDRYLAASIEFLKRNRELGYIPSYDPNWEKDTFASASAVYMRPFMYSEGA
                     IGVGISKAPYVIISATTVSSYFKGGNTKAIITNRIRATPNGTGWIKCAANYIPSALAK
                     KEAEEQGYMEVIFLDAVKRKFVEEGSSCNIFFRLKNGRLVTPALGDTILPGITRESII
                     ALARKEGVTVEERAISIQEVTRSCVECFVTGTAAGITPIESITWKNREYVFNNRRPGE
                     LGSRLQSTLKGTQYGTIADTLSWTVRV"
     gene            3598..>3816
                     /locus_tag="PKH81_01865"
     CDS             3598..>3816
                     /locus_tag="PKH81_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNQ96812.1"
                     /translation="MAINCGIVGLPNVGKSTIFSALTSAPAEAANYPFCTINPNVGIV
                     DVPDSRLAYLTNAFNPKKTIAATVEFVDI"
BASE COUNT         1000 a          845 c          928 g         1043 t
ORIGIN      
        1 accatcgaag ttgccttcaa ccttaccatg gttgaaaacc tcttcgtaga actcggtttt
       61 gctatgccca ccgacactga cgtttctggc gaatggaaga agatttctat gtatggtaac
      121 tacaagatgg atgctatgac tttccacctt attgccatct taaagatgct caaagacgac
      181 aaccagatga acttcggtgc tggcatggac tatgctcttg acggcggaat cggaatcaac
      241 gcagacgttc gctactacaa tgacatctgg aacgctacag tagataatcc agatccagat
      301 gctcgcatca ccttcatggt tggcgcaacc aagggattca gcaacggtct tatcggtgct
      361 ggcctcgaag ttgttagcgc agacgaaacc ggttacatgg ttcccgttcg catggaatac
      421 tggttctaat accctcgcat gcttcggcat gtgtaatggt tagttaaccc aagccctccg
      481 gattttccgg ggggcttttt tattgcagaa gaatagtgaa catgctaaag tgactttatg
      541 atgagtaaag acaaggtact gtacgcccta gtggcgtctt tttttctgct ggcgtcctgt
      601 gccagtaagg tagatacttc cggtgaaccg gatgtaatcg cgatggttga tccctttcaa
      661 gttgctacgt tagggatggg tttaagcgac cttcttggct tggggatgaa tcaaacaacc
      721 tttgatcttt tctttgcccc gcgaggaaac gcgaccatta tggcgttttc gtatcagatg
      781 aataaaacag aggtgtttct ggactatacc gccaggcaga cagtcattgc cgcagttgga
      841 acgtatttgg aagaatatga agcgaaaacc ctgcgaacaa agggcaaaac aaccgctgag
      901 tatggaaaga tgcaagggta tatggaatgg ggcgtgttaa ccaaaaacgc acgggccact
      961 ccggaaattt ctattggtta tgactttgta aaggattccc cgtattttac gctcaccgta
     1021 ccggaaagca caaacttgat atacgaagga gttggcggat cgagagtaca acgctcggcg
     1081 tatatgcggt tttattttaa tcgcgaacaa gcagtggagt ttgcacaaca cctgagtcaa
     1141 gagttcttgc tggaagctct tggcgaacag gatgtaccgg aagcctcgtt tactcccgat
     1201 tcatattgat tttttgaaaa gcaataatat tgaatgttga tggaaaacag gaacgcgtgt
     1261 attgagtgct acttgccctg ttcgtttttt gtaatgagct cgaaatactg tgatggatta
     1321 aaaatgagag gttttgttat ttctacacca tcaaaatcct tgtcgcccgt taacaggata
     1381 tcagaatctg acagaatagc agagacaaga acgggataat cgcatggatc ccggatttga
     1441 ggatattccg tcaacgaaac agattcagga gtgtgaaaaa gttcaaaatt aaggtccaaa
     1501 aagaattgct ccagaagagg gcacttgtca gggaatttgc gtgaaaaaac catagttgct
     1561 tcagcaatac aaaaacttgc tataatcagt tcatggtgtt caataatatg attgaacacc
     1621 accgcagttt ttccttcagg aaagagtatt gaagaaatga gtatgtttgt atcaataaag
     1681 acacgcatca tcaagtagca gtgtttctga tccgtttgat gtactggact acatcgtcct
     1741 ctgtagtgaa accggctttt tttgcttctc cggacattcc gttctggaca gcgcgtatgg
     1801 caacctggga ggcattgaga aactggagcg aaccattgtc ctcaacaaag aagaccttgt
     1861 cgccttcctt taatttgagt ttttttcgta tctcctgcgg tatggtgatt tgaccttttg
     1921 aagtaacctt agcgagttcc atacatacct ccttacattc cttacttaag aataacccag
     1981 gagataatca aattcaagca tatagcggta tatagctggt actatattcg ttcccggtaa
     2041 atcggacccc tttctcctcc ccaggacata tcgcatgcct tccagattca tattgattat
     2101 taacaggtaa tgtcaaagcg tgttgctcct tgcggggtaa catgcttttg ttgtatagag
     2161 accgttgtag aaagcccgtt tccctgcctc agacgcgcgt agagctcctc cagcgcgtta
     2221 accgggccct cagcgtataa ttcaacagaa ccgtcgctcg cgttccgcac ccagccggta
     2281 aggccaagtc gccttgcatc ctgtgcggcc catgcacgaa atcccacccc ctgaactcta
     2341 ccttgaatgg taaacagtat ggcggcgagg acttcgctca tacgcggacg gtccaggaaa
     2401 gggtgtctgc aatagtgcca tattgagttc ccttaagcgt gctttgcaag cgagagccaa
     2461 gctccccggg gcggcggtta ttaaaaacat attctcggtt cttccaggtg atgctctcta
     2521 ttggggtaat gcctgctgca gttccggtaa caaagcattc aacgcaggat ctggtaactt
     2581 cctgaatgga gatagcacgc tcttccacgg ttaccccttc tttccgggcc agggcgataa
     2641 ttgactcgcg ggtaatgccg gggaggatgg tgtctccaag ggcaggggta acaagccttc
     2701 cgtttttaag gcggaaaaag atattacaag aagatccttc ttctacaaac tttcgtttaa
     2761 ccgcatccag gaagatcact tccatatagc cctgctcttc cgcttctttt tttgcaaggg
     2821 ccgacggtat gtagtttgcg gcacatttta tccatccggt accgttggga gttgccctga
     2881 tgcggtttgt aataatcgcc ttggtattgc cacccttaaa atagcttgaa accgtagtgg
     2941 cggagataat tacatagggt gcctttgaaa taccaacgcc gatagctcct tccgaataca
     3001 taaagggccg catgtatacc gcacttgcgg aggcaaaggt atccttttcc cagtttggat
     3061 cgtaggaagg gatgtagcca agctcacggt ttcgcttgag gaattcgatg gaagcggcaa
     3121 ggtatcgatc ttggggaaag gccggggcgt acaagccctt catggaggca ttaaagcgag
     3181 cggcgtttcg gtcaggccta aacagggaaa gagagccatc cttttgggga taggccttca
     3241 ttccttcaaa gcagcccatt gcatactggg ttgtgtagtt gataagcgga agattaaagg
     3301 tgtttcgttt tgcgtatacc ttggattgct ctgcttcgct catggcatca aggtcgtctt
     3361 tggtaatcga gtcggcttga taccagcttt cgtcccaggt gtttgttgca ggattgtact
     3421 gggcgatata ggaaacgggg tacatcgaga gattaaaggc catggtggtt cctccaagga
     3481 taggcgtagt agacagtata aaaatttgtt taatcttggt aaagtagtgt gaacgcttgt
     3541 cctttactcc tttcacggat aaaatgacaa acacagattt gaatctacga ggaatatatg
     3601 gctataaatt gcggcattgt cggtctaccc aatgtgggaa agtccactat tttttcggct
     3661 ctgacgagcg ctccggcaga ggctgccaat tacccttttt gtacaatcaa cccaaatgtt
     3721 ggtatcgtgg acgtaccgga tagccgatta gcctatctta ctaatgcgtt taaccccaag
     3781 aaaacaattg ccgctacggt ggaatttgta gacata
//
LOCUS       DAOQXQ010000070         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24889_length_3794_cov_2.813319, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000070 DAOQXQ010000000
VERSION     DAOQXQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24889_length_3794_cov_2.813319"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKH81_01870"
     CDS             complement(<1..191)
                     /locus_tag="PKH81_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNQ96813.1"
                     /translation="MRKYELMVVFPLEEDQFKTGAEGVRKVLADFGAQIVSEEPYGDR
                     DLTYEIKKRNRGRYVLFTIT"
     gene            384..824
                     /locus_tag="PKH81_01875"
     CDS             384..824
                     /locus_tag="PKH81_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96814.1"
                     /translation="MLQNIVFQDNIYQLSRLIDLVQEGLQLDLSEDFFLDKTLDDLLF
                     FDAALQKMKGQVEGNSRMSDYIPILHSLYSCEERFIRIVDLLLSGKGAMNGAFTQFMT
                     RLQGIRNVHAGIRESLIQGIRKSDNSIDSRDMVSQNELSELLNF"
     gene            840..2126
                     /locus_tag="PKH81_01880"
     CDS             840..2126
                     /locus_tag="PKH81_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ96815.1"
                     /translation="MHTYDCVIVGSGPAGLGCAFELLASNPSASILLLDQEKVSSGGL
                     RNDCKMNFTWPIGFPIEYWEHDLSVEYLERVEAFLNPTIIPQTNLSTYQKRAKRIGVD
                     LLMIRQSHLGTDGGLRLIKRLIEDLSKSGVEIALEEAFVGVDRQNKRCITSKREVGYD
                     QLVVAPGRHGFDFLRKFMRDNDIDYTDNSIDVGVRVETRIEHYPIVKDYYDPKFIFPE
                     RTRTFCTNSGAAHVVQERYTAANGQHYYSVNGHAWSNEREPNGLVNFAILRSVEFTHP
                     LASGQDYAENLAFLAMLTGGGHPIMQRIGDFRLGKRTTTEGLNGDLNDFVPSLPSCTP
                     GDISLAMPAKFLRSIWKALKQLDTIVPGILHPGTILYYPEIKLYANKPVFLDGYFQAS
                     ADIYLIGDGAGTSRGITGAWASGMRAADGIRRKKGW"
     gene            2126..2659
                     /locus_tag="PKH81_01885"
     CDS             2126..2659
                     /locus_tag="PKH81_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNQ96816.1"
                     /translation="MRITGGILCGRTVACPAGVIRPAMDRMRESMFAILGDLEGKSFL
                     DTFSGSGICALEAASRGAHPVFLVEKDPLKVDTIFSNVSIAPHRIECKFMSVELYLKR
                     AKESFDIVYFDPPFPYKYHQDLIHRCGELSILKNGGLAIIHRPKERLLPDTIGKMSRT
                     DERVYGRSIVDFFTKSV"
     gene            2708..3040
                     /locus_tag="PKH81_01890"
     CDS             2708..3040
                     /locus_tag="PKH81_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96817.1"
                     /translation="MDSNALDDFARFPGEGLIKSADEPLGGIQDDRKAALNRKGNELF
                     NQGDVETARRIFVTTGYSDGLTRTGDWYAQKGKLLEALKMYYLAHNRRKADPIIEKLA
                     KLISMTIS"
     gene            3079..>3794
                     /locus_tag="PKH81_01895"
     CDS             3079..>3794
                     /locus_tag="PKH81_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ96818.1"
                     /translation="MPLMPEPVAGTTEEEKKFNEIAELSKNGYLLLKDNKIDEAERAF
                     QKILALEAHNNYALVGLGDAARKKNRFRDAVEHYTDCLSHHPGNNYALFGLADCYKAL
                     NQYNKAIEIWEQYLQHDDRNITVLTRVADAYRKIRDFKQSKILYLRVLDMEENNPYAL
                     IGLGHLHYDFKEYRDALYYWQKMLDMNEDSVDIRVLTSIGNCHRKLKTFEQGIYYFQR
                     ALRMDENNFYALFGLADCYRG"
BASE COUNT         1060 a          852 c          843 g         1039 t
ORIGIN      
        1 gttatggtaa acaacacata acgaccccga tttcgtttct tgatttcgta ggttaaatcg
       61 cgatcaccat atggttcttc gcttacaatc tgcgcaccga aatcggcaag taccttgcgt
      121 actccttcgg caccggtttt gaactggtct tcctctaacg gaaaaacgac catcagttca
      181 tactttctca tgtttcctcc tgtggacaca tttctgggcc cgttttcatc gtaccatttg
      241 ggtatcagtc gaaacgggct ggagttttgt tgctgaattg acaaaatata ccaatccaga
      301 gcgtttatcc tagcagtttg accgtctttg gtcaaggata cgggtatttt gccgatattt
      361 ttaaaggata tccaggagga aacatgttac agaatatcgt ttttcaggac aatatatatc
      421 agctcagtcg cttaatcgat cttgtgcaag aaggcttaca acttgatctt tccgaagatt
      481 tttttctgga taagacactc gatgatctgt tattctttga cgctgccttg caaaaaatga
      541 aaggacaggt tgaaggcaat tcccgcatgt ccgattatat tccaattctt catagcctct
      601 attcgtgtga agagcgcttt atcaggattg tagacttact gctctctggt aagggtgcta
      661 tgaacggcgc atttacccaa ttcatgactc gacttcaggg aattcgcaat gttcacgcag
      721 gaattcgtga atctcttatt cagggcatca gaaaaagtga taattcgatc gattcccgtg
      781 atatggtatc ccagaatgag ttgagtgaac ttctcaattt ctgatatact tatgatcgta
      841 tgcataccta tgattgtgta atcgtcggat cgggccctgc cgggcttggt tgcgcctttg
      901 aactacttgc ttcgaatccg tcggcatcaa ttctcctttt ggaccaggaa aaggtatcct
      961 caggtggatt gagaaacgat tgcaagatga attttacctg gcctattggc ttccccattg
     1021 agtattggga acacgatcta agcgtggaat accttgaacg tgtcgaagca tttttaaacc
     1081 cgaccatcat cccccaaaca aatctttcaa cctatcaaaa aagagcaaaa cgcatcggtg
     1141 ttgatctatt gatgattcga caatcccatt taggcaccga cggtggcttg cggttaatta
     1201 aacgccttat tgaggatcta tctaaaagcg gtgtagaaat tgcccttgag gaagcctttg
     1261 taggtgtgga tcgacaaaac aagcgctgta tcacctccaa acgagaagtt ggatatgatc
     1321 aacttgtggt agcacccggt cgacatggct ttgatttttt acgcaaattc atgcgggata
     1381 atgatataga ttacaccgac aacagtattg atgttggagt tcgggtagag acaagaatcg
     1441 aacactatcc gattgttaaa gattactacg atcccaagtt tatttttccc gagcggacca
     1501 gaaccttttg taccaattca ggagcagccc atgttgtaca agagcgttat accgccgcta
     1561 atgggcagca ttactacagt gtaaatgggc atgcctggtc caatgaacga gaacctaatg
     1621 gtttggtcaa tttcgccatt ctcagaagtg ttgaatttac ccaccccctg gcctctggcc
     1681 aggattacgc agaaaatctt gcatttcttg ccatgctaac cggtggaggc caccccatca
     1741 tgcaacgaat cggtgatttt cgcttaggaa agcgaacaac caccgaaggg ctcaatggag
     1801 acctgaacga ctttgtaccg tcactcccct cctgcactcc gggagatatc agtttggcca
     1861 tgcctgcaaa gttcctcaga tcaatttgga aggcgcttaa gcagctcgac accattgttc
     1921 ccggtatact gcatccaggg accattctct attatccaga aatcaagctt tatgccaata
     1981 aaccggtatt tttggacgga tattttcagg catctgctga tatctacctt atcggtgatg
     2041 gagccggcac atccagaggc attaccggag cgtgggcttc aggaatgcgc gctgcagatg
     2101 gaatccgccg aaagaagggc tggtaatgcg tattaccgga ggaatcttgt gcggaagaac
     2161 agttgcatgc cctgcagggg tgattagacc tgctatggac cgaatgaggg aatctatgtt
     2221 tgccattctt ggcgatcttg aaggaaaatc attcctggat accttcagcg gttcaggtat
     2281 ttgcgccctt gaagctgcat cccggggagc acacccggtt tttttggttg aaaaagatcc
     2341 gctcaaggta gataccatct tttccaatgt ttctatagcc ccacatcgta ttgaatgtaa
     2401 gttcatgtct gtagaactct atctaaaacg cgcaaaagaa agcttcgata tcgtttattt
     2461 tgatcctccc ttcccctaca agtatcatca agacctgatt catcgctgcg gtgaattatc
     2521 cattttaaaa aacggtggat tggcaataat ccatcgccca aaggagcgat tactacccga
     2581 tactattgga aagatgagcc gtacagatga gcgagtctat gggcgatcta ttgtggactt
     2641 tttcacaaaa agcgtgtaac tattgcacac catcagcaaa tttcttatta tataaacagg
     2701 gttgccaatg gattcaaatg ctcttgacga ttttgcccgt tttcccggcg aaggccttat
     2761 caagagtgcc gatgaaccac ttggtggaat tcaggatgac agaaaagctg cccttaaccg
     2821 taagggtaac gagcttttta accagggtga tgtagaaacg gctcgccgga tctttgttac
     2881 taccgggtat tcggatggac tgaccagaac tggtgactgg tatgcacaaa aagggaagct
     2941 tcttgaagca ctcaagatgt attacctggc gcacaaccgg agaaaggcag accccataat
     3001 tgaaaaattg gcaaagctca tttcaatgac aatttcatga taaacaaaga ggcaaacatg
     3061 gtagacataa tgcaggatat gcccctgatg ccggaaccgg tagcagggac caccgaagaa
     3121 gaaaaaaaat ttaacgagat agccgagctt tcaaaaaatg gatatctctt gcttaaagac
     3181 aacaagattg acgaggctga acgagccttt cagaaaattc tggcccttga agctcataac
     3241 aactatgccc ttgttggcct cggagacgca gcccgcaaga aaaaccgctt tagagacgct
     3301 gtagaacact atacagactg tctttctcat catcccggaa ataactatgc tcttttcggc
     3361 ttggctgatt gctataaggc tctcaatcaa tacaacaagg ctatagaaat ttgggaacag
     3421 taccttcagc atgatgaccg taacatcacc gtacttactc gcgtagcaga tgcctaccga
     3481 aaaataaggg attttaaaca gtccaaaata ctctacctga gagtcttgga catggaagaa
     3541 aacaacccct atgccctgat tggacttggg catcttcact acgattttaa ggaatatcgg
     3601 gatgccctct attattggca aaaaatgctc gatatgaacg aagactctgt ggatattcgc
     3661 gtccttacct ccataggtaa ctgccatcgc aagttaaaaa cttttgaaca aggtatttac
     3721 tattttcagc gggcgctcag aatggacgag aataacttct atgccctgtt tggtcttgca
     3781 gactgttacc gggg
//
LOCUS       DAOQXQ010000071         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_24893_length_3793_cov_3.643392, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000071 DAOQXQ010000000
VERSION     DAOQXQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24893_length_3793_cov_3.643392"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PKH81_01900"
     CDS             <1..1228
                     /locus_tag="PKH81_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ96819.1"
                     /translation="LLFSSRDFISMLPDVSVSWSGGTSIEKDGPDSRTKEISIAATQV
                     VYDGGKRLLEYKMGKEKSIREWNMLAEENEEYLGSVRSAYITLSLMLEISAIQRNVLD
                     AAITQEEIAKHEWELGRIIETDYLEVSSERFSRESEWLLSEYQAQRQRRVLLSLMGVD
                     WRASVTVIPIKTPMPEQFNILEPYYLRIVSRINKESLEIESMERDIAYRELLMKWDER
                     KALPSISVQGEIGFSGEDYPLGNPVYTLRCNIALQNRLFPVSASTGVIRDAERVKRVS
                     GSSRIEILPSITFMNEKRISRLSLESDKAHLRELSSKLDSDAFDTLYEHDALVRDYQI
                     RAQQMNILQRKNAADKTKLSNGVIRRIDYLLAENDWAQAKTKYVDTAFKIIEIEDQIS
                     RRMGLSLMEVCNNDAH"
     gene            1215..2561
                     /locus_tag="PKH81_01905"
     CDS             1215..2561
                     /locus_tag="PKH81_01905"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ96820.1"
                     /translation="MMHINRYILSITIILLVVFLSSCVRATEKETSEPASVSVTEASI
                     GEYRQSVSCFGSISYKNKWDVSSEAEGILNFSMLKEGQQIIKGQVLCTIDNPYIVAQY
                     EQAQFALETARSSLANAESRLYSRMLEVESRFCSLEQYRLQTDKLRMQIESIARELAI
                     KESLHKVGGITDEELISSRESLLSSQMDLEILISEQTIAEIGYRSSDITANGLTPSCD
                     PDEFMRQCIELNSRAERSNVEAAQAEIRIAMGKKESARLMKDTLIVRSPASGVVAAVF
                     HESGEYIQQYDKIVTVLDTSGIYAVFPVSETDVLFLNTNTTINLEIPSVSLSLESTGT
                     LVSPIADPGSGIFSIKVPLDNKNGTCKPGMFARCTFTRGDVETLIHIPDTALLYEREG
                     IGKVYCVLDSTAVLHEVYIRAREKGIVWIASGLEKGVQVIDSPPPYIREGSHVIAN"
     gene            2545..>3793
                     /locus_tag="PKH81_01910"
     CDS             2545..>3793
                     /locus_tag="PKH81_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ96821.1"
                     /translation="MLLQIRLLTFLFCIGFCISCSIVSFDQLEIESDVQGYNNVFTKE
                     EVTLQFSMTPSNGSVEKILVLRDEQGVLACKYDWNGRTLKIKPDSGWRKGGEYQLLIK
                     GTIDCDDGPSSRVSFEAAWIQDSDSDYLSLLSVSPGSDHVFQDLDSILFEWSSPIDID
                     VFTSAISFSPPLSHKVDISNDGRLCTLTPSTPWKGDCVYEWKIEKMTAVSGFSQKVPV
                     GGIVNTVVTNGLPEISVICPVEKSSGQWFTNLPLDGYMLSDRSIGIQFSHPMDNATVS
                     AVRIEPSVAGYVRQTTSCDFQWFPLEKFIQGVRYCLIISPAALDIWGHHCQEERREWF
                     TSSLLPFGISSIRINEVHELINPSGIKNEMECNGIEGRLSAVIQFSSPIEEHMKQSVC
                     DKITLPSYYPSSISYPIIESITWN"
BASE COUNT         1151 a          646 c          837 g         1159 t
ORIGIN      
        1 tcttttattt tcttccagag attttatatc aatgttgcct gatgtgtctg tttcctggtc
       61 tggagggact tcaatcgaga aagatggccc agattcgcga acaaaagaga taagcattgc
      121 ggcaactcag gtagtttacg atggaggaaa acgtttactt gaatacaaaa tgggtaagga
      181 aaaaagtatt cgtgaatgga acatgcttgc agaagaaaat gaagaatacc ttggttcagt
      241 gcgttctgcg tatattaccc tttctttgat gctggagatt tctgctatac agcgtaatgt
      301 tcttgatgca gctattacac aagaagagat agctaaacat gaatgggagc taggacgtat
      361 tatagaaaca gactatttag aagtatcatc tgaacgattt tccagggaga gtgaatggct
      421 gctctctgaa tatcaagctc aacgacaacg tcgtgtgttg ttatcattga tgggagtaga
      481 ttggcgtgct tcagttacag taattcctat taaaacgcct atgccagaac aatttaatat
      541 ccttgaaccg tattatctgc gtatcgtatc cagaataaac aaagagagtc tagaaataga
      601 atcaatggag cgagatattg cgtatcgtga acttttaatg aaatgggatg aacgaaaggc
      661 gctaccttca ataagcgtac aaggtgaaat aggtttttct ggagaagatt atccgttagg
      721 taatcctgta tatacgcttc gctgcaatat agctttgcaa aatcgcttat ttcctgtaag
      781 cgcgagtact ggtgttatcc gtgatgctga aagagtaaaa agagttagtg gtagttccag
      841 aattgaaata ttaccaagta ttacctttat gaacgaaaag cgaataagtc gattatctct
      901 tgaatctgat aaagcacatc ttcgtgaatt atcaagcaag cttgatagtg atgcttttga
      961 tacgttgtat gaacatgatg ctttagtaag agattatcag atacgggcac agcaaatgaa
     1021 tatactgcaa agaaaaaatg ccgctgataa aacaaaatta tcaaatgggg tgattagacg
     1081 tattgattac ctgcttgctg aaaatgactg ggctcaagca aaaactaagt atgttgatac
     1141 cgcatttaag ataattgaaa tagaagacca aatttctcga cgaatgggac tttcgcttat
     1201 ggaggtttgc aataatgatg cacattaatc gatatatttt atcaattact attatattgt
     1261 tagttgtttt tttgtcctct tgtgtacgag ctacagaaaa agaaacaagc gaaccagctt
     1321 ctgtttctgt cacagaagct tctattggtg agtaccgtca aagtgtttct tgttttggct
     1381 caatttcgta taaaaataaa tgggatgtat caagtgaagc tgaaggtatc cttaatttca
     1441 gcatgcttaa agaaggacaa caaattataa aaggtcaagt gttatgtaca atcgataatc
     1501 cttatattgt tgcacaatac gagcaagctc aattcgcgct tgaaacagca cgatcatcgc
     1561 tggctaatgc tgaatcccgt ctttattcac gtatgcttga agttgaatca cgattttgct
     1621 cattggaaca gtatcggtta caaacagata agcttcgcat gcaaattgaa tcaatagcga
     1681 gggagctggc tataaaggaa tctttacaca aagtgggcgg tattactgat gaagaattaa
     1741 tttcttcgcg tgaatccctt ttgtcttctc agatggatct tgagatactt atttccgaac
     1801 agacgatagc ggagattggt tatcgttcaa gtgatattac agctaatgga ttaacaccat
     1861 cctgtgatcc tgatgagttc atgcgtcagt gtattgaatt aaactctcgg gcagaaagat
     1921 ctaatgtaga agctgcgcaa gcggaaatac gaattgcaat ggggaaaaaa gaaagtgcga
     1981 gattaatgaa agataccctg atcgtacgat ctccagcaag cggagttgtt gctgcggttt
     2041 tccatgagtc aggcgaatat attcaacagt atgataagat agtaaccgta ttggatacta
     2101 gtggcattta tgcggtattc ccggtatctg aaacggatgt tctatttctt aatacaaata
     2161 caaccataaa tcttgaaata ccttctgtgt ctttatcttt agaatctaca ggaaccttag
     2221 tatcgccgat tgcagatccg ggaagtggta ttttttcgat caaggtacct ttggataata
     2281 aaaatggaac atgcaagccg ggaatgttcg ctcgctgtac atttaccaga ggggatgtag
     2341 aaacattgat acacatccct gatacggctc ttctctatga acgtgaaggt attggtaagg
     2401 tctattgcgt tctcgatagc actgctgttc ttcatgaggt gtatattcga gcccgcgaga
     2461 aagggattgt atggatagcc tccgggttgg aaaagggcgt gcaagtgatc gattcgcctc
     2521 cgccatatat aagggaggga tcacatgtta ttgcaaatta gattgttgac gttccttttt
     2581 tgtattggtt tttgtatatc atgctcaata gtgtcttttg atcagttgga aattgaatcg
     2641 gatgtgcaag gctacaataa tgtatttaca aaggaagaag taacactaca gttttcaatg
     2701 actccttcaa acggcagtgt agaaaaaata ctcgtgctcc gtgacgaaca gggagttctt
     2761 gcctgtaagt atgactggaa tggacgaaca ctaaagatta aacctgactc tggatggcga
     2821 aaggggggcg agtatcaatt actaatcaag ggaaccattg attgtgatga tggtccgtct
     2881 tcgcgagtgt cttttgaagc ggcatggata caggattcag attctgatta cttgtctttg
     2941 ctttcggtgt ctcctggttc tgatcatgta tttcaagatt tagattccat tttatttgaa
     3001 tggagttcgc caattgatat tgatgttttt acttctgcga tatcattttc gcctccatta
     3061 tcacataaag ttgacattag caatgatgga agattatgca cactaactcc aagtacgccc
     3121 tggaagggtg attgtgtgta tgagtggaaa atagaaaaga tgacagctgt ttccgggttt
     3181 tcacaaaaag tacctgtggg cggtatagtc aatactgtag ttactaatgg tcttcctgaa
     3241 atatctgtaa tatgtccagt tgagaaaagt agcgggcaat ggtttacaaa cttgcccctt
     3301 gatggatata tgctttctga tagatctatt ggaatacaat tttctcatcc catggataat
     3361 gctactgttt cggctgtccg catagaaccg tcagtcgcgg gttatgtcag acaaacaaca
     3421 tcctgtgact ttcagtggtt tccgcttgaa aagttcatcc agggtgttcg ctattgcttg
     3481 ataattagtc ctgccgccct ggatatatgg ggacatcatt gtcaagaaga aagacgcgag
     3541 tggtttacca gctctttgtt gcctttcggg atctcatcaa tacgaattaa tgaagtacat
     3601 gaattaatca atccttctgg cattaaaaat gaaatggaat gtaatgggat cgaaggtcga
     3661 ttatcagcgg taattcaatt ttcatcaccg attgaagaac atatgaaaca atcagtttgt
     3721 gacaagatta cattgccatc ttattaccct tcaagtatta gttatccaat aatcgaatcg
     3781 attacctgga ata
//
LOCUS       DAOQXQ010000072         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25153_length_3768_cov_2.695125, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000072 DAOQXQ010000000
VERSION     DAOQXQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25153_length_3768_cov_2.695125"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKH81_01915"
     CDS             complement(<1..1071)
                     /locus_tag="PKH81_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96822.1"
                     /translation="MKRRIRVLITISVCYVLKAFSQEVSLADLSFQSTAFIQEEDDSP
                     VMLTGGTIALRFMPVAPIELTARTAFYTQDTQKFLSSFDNSQEPAFVLFEGAALSYTA
                     LFNRTSTVTVFTGLLDNPASPSLLRRLFKITVEPCEFHGMPAGVPFSPETEIRGNGLA
                     LTTIPFNTQVGAGIYTYWNGETGSQFTLTGDLRLVLALDNAFFNFFGGSSWLYGDEKA
                     SFRGGATALVRSDSGHELYGEAGIRSISIGSDDIGKNLYFLFESRLHTNAIDSVFSFF
                     SSPVFPSTLRNESIMEAETMFLGLNALIAWGRLPIERIRTGISFLGALNPDDPGTVTP
                     FSFSVSPFVTCRLSDYDIQVTAV"
     gene            1197..3572
                     /locus_tag="PKH81_01920"
     CDS             1197..3572
                     /locus_tag="PKH81_01920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNQ96823.1"
                     /translation="MSDYLDINNEELLKDFFSEAEQQVFTLESNILVIENDPSNHEAI
                     DEIFRAAHTLKGGSATVEMHELSGFTHIVEDLLDVIRSGSLAVSGSVVDILLQALDII
                     KAMLSARSAGAVFDDDVSPVKEAIEALLPSKEKKQKPAAAKKKVPAPSSVAPRTVEMV
                     PSADTTALLSEYEILELKEAVPPGESVFIVRVTFDESNPMNSVGGIQVFAALKQHGQV
                     LKTIPDFDALYEDEFHEHVVYFVATTVSSEDLKKASSISDVTSSVDVQPFMAQAQPQR
                     SVPAPKPAVPVVSRKEESPVEVDEEESVGEPKAMTELSEKTPSKSASTVSHNSSGSIL
                     RVDSRRIDYLLNLVSETVITKASFNQSALHFSDLISEFQQAEGAWKERLRALLDSIPD
                     LLEQIQDGRSVKDIKKELMAEYNGLFEVFGGFDSSMKGTVSKFRSAAQNLGRITGELQ
                     EGVMKIRMVPISQIFNRFPRLVRDLSHDLGKQVRLVIEGEDTELDKSVVEDLLDPIMH
                     CVRNAIDHGVELPSVRAAAGKSEEGMVLLKASNEGNMIVIEIADDGHGIDVESVKKKA
                     VARGIIHPGKNLTDVEAFQLIFEPGFSTAEKITNVSGRGVGLDVVKTHIEKLNGTVTI
                     SSQPNIGSRFVIRLPLTLAIIQGLLVRVGREVYSIPITSVIESHRVKPEEISRIDNYE
                     VFNVRNEVISLLRLNRLFGIKTQDVSEFNYIVIVGTSEKKIGLMVDSLIGEEDVVIKP
                     LRDQFTNSPGIAGASILGDGSVSLIIDVSQLLDLGLKQELQAREQREASVW"
     gene            3566..>3768
                     /locus_tag="PKH81_01925"
     CDS             3566..>3768
                     /locus_tag="PKH81_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNQ96824.1"
                     /translation="MVNKKETRTLVNEDTEREESEVRAEKAVVIDFKMVTFSLAGKDY
                     AIDIMQVKEIAKAGRFTYVPNTSP"
BASE COUNT         1021 a          847 c          931 g          969 t
ORIGIN      
        1 cacagccgtt acctgtatat cataatccga aagccggcag gtaacaaatg ggcttacgga
       61 aaatgaaaac ggagttaccg ttcccggatc atcgggattc aaggctccta aaaaactaat
      121 gccagtgcga atccgctcta ttggcagcct tccccatgcg atcagagcat ttaagcccaa
      181 gaacatagtt tcggcttcca taatggattc gtttctgagc gtagagggaa acacggggga
      241 tgaaaaaaaa gaaaacaccg aatcaatggc gttagtatgc aaacggcttt caaataaaaa
      301 gtagaggttc ttcccgatgt cgtcacttcc aatgctgatg cttcggattc ctgcttcccc
      361 atagagctca tgtccgctgt cagatcgaac cagggcggtt gcgcctccgc ggaaagaagc
      421 cttttcatcc ccatacagcc aggaactacc cccaaaaaag ttaaaaaagg cgttatccag
      481 ggcaagtacc aaacgaaggt caccggtaag ggtgaactgt gaacccgttt caccgttcca
      541 gtaggtatat ataccggccc ctacctgtgt attaaaagga atggtcgtaa gggctagtcc
      601 gtttccccgt atctcggttt ccggcgaaaa aggaacaccg gcgggcatcc cgtgaaattc
      661 gcatggttcg acagtaatct tgaataaacg gcgcagcaga gagggggagg cgggattgtc
      721 gagtaggccg gtaaagacgg tcactgtgct tgttcgattg aacagggctg tataggacag
      781 ggcggctcct tcaaacaaga caaaagccgg ttcttgactg ttgtcaaacg acgataaaaa
      841 cttttgcgtg tcttgggtat aaaaagcagt gcgggcggtc agttcaatag gggctaccgg
      901 cataaagcgg agggctattg tacccccggt gagcattacc ggactgtcat cttcttcttg
      961 aataaaagcg gtcgattgaa aagagaggtc ggcaagtgaa acttcttggg aaaatgcctt
     1021 aaggacgtaa catacgctga ttgtgataag tacccggatg cgtcttttca ttcaaactcc
     1081 ctgtctcagt atataccatt tggaagcggc agagccacta tttccttttc aaaaaggaaa
     1141 aaccctcctg gatttcgtct tgactaatcg tcaaaacggt atacacttga tatcatatga
     1201 gtgactatct ggatatcaac aatgaggaac tactaaaaga ctttttcagt gaggctgaac
     1261 agcaggtttt cacgctggaa agcaatatcc tggttataga aaatgatcca tccaatcatg
     1321 aggctatcga cgagattttt agagctgcgc atacccttaa gggtggctct gctaccgtcg
     1381 aaatgcacga actgtcgggt tttacgcata ttgtagaaga cttactcgat gtgattcgct
     1441 ccggttctct tgccgtttcc ggctctgttg tggatattct tctacaggcc ttggacatca
     1501 tcaaggcaat gttgtctgcc aggtctgccg gtgctgtgtt tgatgatgat gtttccccgg
     1561 taaaagaggc tattgaagcc ctgcttccct caaaagagaa gaagcaaaag cctgcggcag
     1621 ccaagaaaaa agttcctgca ccatcttcag tcgctcctcg aacagtcgag atggttccct
     1681 ctgccgatac aacggctctg ttatcggagt atgagatcct tgagcttaaa gaagctgttc
     1741 ctccgggtga aagtgtcttt attgtgcggg ttaccttcga tgagtctaac cccatgaatt
     1801 ccgttggtgg aattcaggtt tttgccgctc ttaagcaaca cggtcaggtg ttaaagacta
     1861 ttccggattt tgatgccctc tacgaggatg aattccacga acatgttgtg tattttgtgg
     1921 caaccactgt ttcaagcgag gatcttaaaa aggcctcttc tatttccgac gttaccagtt
     1981 cggtggatgt gcagcctttt atggctcagg cacaaccaca acgaagtgta ccagcgccaa
     2041 aaccggcggt gccggtagtt tcccgtaaag aggaatcgcc agttgaagta gacgaggaag
     2101 agagcgtcgg cgagccaaag gcaatgacgg aactatcgga gaaaacaccg tcaaagagcg
     2161 catctacggt atcccataat agttcaggct ctattcttcg agttgattcc cgtagaatcg
     2221 attatttgct taatctggtg agcgaaacgg ttattaccaa ggcttccttt aatcaaagcg
     2281 ccttgcattt tagcgatctt atttccgagt ttcagcaagc cgaaggggct tggaaggaac
     2341 gactgagagc cctgcttgat tctattccgg atttgcttga acagattcag gatggtcgaa
     2401 gcgttaagga tatcaagaaa gaattaatgg ccgaatataa cggtcttttt gaggtttttg
     2461 gtggttttga ttcctctatg aagggtaccg tcagtaaatt ccgctccgcc gcgcaaaacc
     2521 ttggtagaat taccggtgaa ttgcaggaag gcgtaatgaa gatccgcatg gttcccatca
     2581 gccagatatt taaccgcttc ccgcgccttg tccgtgattt atcacatgac ttgggcaaac
     2641 aggttcgtct ggtcatagaa ggagaggata ccgagctcga taaatccgtt gtagaagact
     2701 tgcttgatcc cattatgcac tgtgtgcgca atgcaattga ccatggcgtt gaacttccct
     2761 ctgtcagggc tgctgccggt aaatccgagg aaggtatggt cttgctcaag gcgagcaacg
     2821 aaggcaacat gattgttatc gaaatagccg atgacggtca tggaatcgat gttgaatcgg
     2881 tcaagaaaaa ggcagtcgct cggggcatca tacatcctgg taaaaacctt accgatgtgg
     2941 aagcctttca gcttattttt gaacctggtt tttccacagc cgagaagatt accaatgtat
     3001 ccgggcgcgg agttggcctc gatgtagtaa aaacccatat cgagaaactc aacggaaccg
     3061 tcactatttc aagtcaacct aacatcggat cacgttttgt tatccgtttg ccacttaccc
     3121 ttgccataat ccaggggctt ctggtgcgcg ttggccgcga ggtgtactcc ataccgatta
     3181 catctgttat cgaaagccat cgtgtcaaac cggaagagat tagccgtatc gataactatg
     3241 aagtctttaa tgttcgtaac gaggtaatca gcctcttgcg tcttaaccga ctttttggca
     3301 taaagacaca ggatgtttcc gagtttaact atattgttat cgtaggaaca agcgagaaaa
     3361 aaattggttt gatggtcgac agtcttattg gagaagagga tgtggtaata aagcctcttc
     3421 gggatcagtt taccaattcg cccggtattg ccggtgcctc gattttggga gacggttcag
     3481 tatccctcat catagacgtt agtcagcttc tggatcttgg acttaaacag gagttgcagg
     3541 cgcgcgaaca acgtgaggct tcagtatggt aaataaaaaa gaaacgcgaa ccctggtaaa
     3601 cgaagatact gaacgggaag aaagcgaagt acgcgccgaa aaggcagttg tcattgattt
     3661 caagatggtt accttttccc ttgccgggaa agattatgcc atcgacatca tgcaagttaa
     3721 ggaaatcgcc aaggcaggac gttttaccta cgtccccaac acctctcc
//
LOCUS       DAOQXQ010000073         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25363_length_3747_cov_2.871343, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000073 DAOQXQ010000000
VERSION     DAOQXQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25363_length_3747_cov_2.871343"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKH81_01930"
     CDS             <1..516
                     /locus_tag="PKH81_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96825.1"
                     /translation="KRRLHSSFSTTRFFEMSTTEMARYLKKNSFHVIACLDNTLPYIA
                     DLTLLRKFFHDAKTLLTQDGKLIIHCLNADGLAESKPVRLPERSSIRVRLSQAYIPAN
                     NGEMTFQTSLELGNGKTISLKKGIPIQILSTTKIMDLAREAGFTRITVWGDYRKGSFT
                     SSSENAVIMCS"
     gene            complement(526..1443)
                     /gene="ricT"
                     /locus_tag="PKH81_01935"
     CDS             complement(526..1443)
                     /gene="ricT"
                     /locus_tag="PKH81_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNQ96826.1"
                     /translation="MASAYEDDIRSEDLSSLEDPQVESMDGGSEHYVFPDPLYHLKLE
                     YSSESLYATHDSLDFNAGDHVFVPTRYGKDIALVMGQVRIPIGIRPEDVVVIDHRADD
                     HDLAHMAENREKEAKAFSVFREKVAQHKLDMKLIATHYLLDEPKILFFFSAENRVDFR
                     DLVKDLVSIFKMRIELRQIGVRDESRITGGLGVCGRPYCCNSVTDKLKPVSIRMAKDQ
                     SLSLNSLKISGQCGRLLCCLSYEHEWYCEAKKNLPNEGVRIFYDEVNFRVSEVNLLTG
                     MIRLHGEDGRILDIPSSRMTRIDGKWKIQ"
     gene            complement(1471..1998)
                     /locus_tag="PKH81_01940"
     CDS             complement(1471..1998)
                     /locus_tag="PKH81_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HNQ96827.1"
                     /translation="MANPVDPVSPFFSSIAGQAGISALGAKAAGAKKNKEPVKRFDRL
                     VQETLSTESEAQNIVLPERLQGLSSEKILEGLLDDVHSAGDALKERSLPEHIRDYKNA
                     VRFFVRYVVDKSFTVTETTSGGNILKRKKFTQIQVIDEKLEQLAREILSNQRNQMAVL
                     ARIEEINGLLVDLLS"
     gene            complement(1998..2441)
                     /locus_tag="PKH81_01945"
     CDS             complement(1998..2441)
                     /locus_tag="PKH81_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNQ96828.1"
                     /translation="MAFYSAEDVSINTIIGPGTRINGELSVAGFVRVDGDVDGNVDTA
                     GRVIVGENARIRGSVHGKEITVGGVIQGDVIAPEGVRILSSGMVLGDVLTKKLLVEES
                     VILNGRCFAVNNVQEFEAALSDYRNRMALSDTAFSFLRSPGSGSA"
     gene            complement(2451..3476)
                     /locus_tag="PKH81_01950"
     CDS             complement(2451..3476)
                     /locus_tag="PKH81_01950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ96829.1"
                     /translation="MPKTRGYKRVENTIFRSIGRSLCKGASSVHGVCSSISQGGQRKL
                     TIMVVPHSQKRVINFQTTVFSLLFFAVALTGIFGSFFWFSRRAIVSEQNLTELREEAR
                     TARASLDELKDETGNLFKAAQNFQAAFSGTLSLIGMDNAQPLKSNIQNGDLSSLFNVK
                     ETAQGSVREIAELKHLTGYLENSVQPIQEIGKLLESQSTLFSDIPSLWPIRGGIGHVS
                     MAFGQNRHPFTGQWYIHKGVDLSTYRAGDSILATADGQVVTAEYDPGFGYYLIIKHKH
                     GFYTRYAHMQSFRVQRGQYVQQGQIIGYIGNTGVSTGPHLHYEVHIGSDVVDPMKYLN
                     IKVASGK"
BASE COUNT         1058 a          928 c          756 g         1005 t
ORIGIN      
        1 aaacggagac tacacagctc attttccaca acgcgctttt ttgaaatgtc caccaccgag
       61 atggccagat acctaaaaaa gaactctttt cacgttattg cctgtctgga caatacactg
      121 ccttatatcg cggaccttac cctcctgcgc aagttcttcc atgacgcaaa aaccttgctt
      181 acacaagatg gcaaactgat tattcactgt ctgaacgcag atggtttggc agaatcaaag
      241 ccagttcgcc tgcctgaacg ttcatcaatt cgggttcggc tgtcccaagc atatattccg
      301 gctaataacg gggagatgac gttccagaca agccttgagt taggcaatgg taaaaccata
      361 tccctaaaaa aagggattcc tatacaaatc ctctccacta caaagattat ggatcttgcc
      421 agggaagcag gatttacacg aatcacggtg tggggagatt atcgtaaagg gagctttacc
      481 tcttcaagtg agaatgcagt aattatgtgt tcttaacagg agccgttact gtatcttcca
      541 tttcccgtca attcgcgtca ttcgggaaga aggaatgtca agaatacgtc cgtcctctcc
      601 atgaagtcgg atcatgccgg taagaagatt aacctcgcta acccggaaat ttacctcatc
      661 ataaaagatg cgcacgccct cattgggaag gtttttcttt gcttcacaat accattcatg
      721 ctcgtaagaa agacaacaaa gcaaacgacc acactgtccg gaaattttaa gagaattaag
      781 cgacaggctc tgatctttcg ccattcgtat agacactggt ttcagtttgt cggtaacaga
      841 attgcaacaa tagggcctgc cgcacacccc caagccacct gttatacggg actcgtctct
      901 cacaccgatt tggcgcagtt caatgcgcat tttgaatatg gatactaaat cctttaccag
      961 atctctaaaa tccacccgat tttcggcact aaagaaaaag agtatttttg gttcatcaag
     1021 caggtagtgt gttgcaatca gcttcatatc caatttgtgt tgggcaacct tctctcgaaa
     1081 aacggaaaag gcttttgcct ctttttctcg attttctgcc atatgagcta aatcatgatc
     1141 atcggcccga tgatctatta caacaacatc ttcaggacga atccctatcg gaatgcgcac
     1201 ctgccccatc actaaggcaa tatcctttcc gtaacgcgtg ggaacaaaaa catgatcccc
     1261 tgcattgaaa tcaagactat cgtgtgttgc atacaagctt tcgcttgaat actccaactt
     1321 cagatgatat aaggggtcgg gaaacacata gtgttcagaa cccccatcca ttgattctac
     1381 ttgaggatcc tccaagctgg acaaatcctc tgatcgtata tcgtcttcgt atgcagaagc
     1441 catcttttcc acctttctct tcggtttact ctaggataac aaatccacca gtaatccgtt
     1501 gatttcttcg atccgtgcca atacagccat ttgattgcgt tgattggaca gaatctcgcg
     1561 agctaactgc tctaattttt cgtcaattac ctgaatttgc gtgaatttct ttcgcttcag
     1621 gatattaccg cccgaggttg tttcggtaac ggtaaaactt ttatcaacaa cgtaccgtac
     1681 aaaaaatcga acggcatttt tgtagtcccg gatgtgttcc ggcaaggatc tttcttttag
     1741 ggcatctcct gctgaatgta catcatccaa caagccttca agaatctttt ctgaagaaag
     1801 accttgtaat cgttccggca gaacgatgtt ctgtgcttca ctttcggttg aaagcgtttc
     1861 ttgtacaagc cgatcaaatc gttttaccgg ctctttattt tttttagccc ccgcagcctt
     1921 tgctcccaac gcagaaatgc cagcctgtcc cgcaatggag gaaaaaaaag gagaaacggg
     1981 atctacagga tttgccatca ggcagagccg ctaccaggcg aacgtaaaaa agaaaaagcg
     2041 gtatccgaca atgccatccg gttgcgataa tcggaaagag ccgcttcaaa ttcttgtaca
     2101 ttatttaccg cgaagcacct tccattaaga atgaccgact cttctacaag caacttttta
     2161 gtaagtacat caccaagaac catgccactg gaaagaatgc gaaccccttc cggagcaata
     2221 acgtctccct ggattacacc tccaacggtg atttctttcc catggacact tccccgaata
     2281 cgggcgtttt ccccaacaat aacccgccct gcagtgtcta catttccatc tacatcgccg
     2341 tctacacgga caaagcccgc gacagacaat tcaccgttaa tacgagtgcc gggaccaata
     2401 attgtgttga ttgaaacatc ttctgcggag taaaaagcca tcaaaagccc ctatttgcca
     2461 ctggcgacct tgatgtttaa atacttcatg gggtctacaa catccgatcc aatatggacc
     2521 tcgtagtgaa ggtgtggccc ggttgaaaca ccggtattac caatatagcc aattatctga
     2581 ccttgttgta catactgacc acgctgaaca cggaatgact gcatgtgggc ataacgtgta
     2641 taaaaaccat gtttatgttt aataatcagg tagtaaccaa aaccagggtc atactctgcg
     2701 gtaaccacct ggccgtctgc agtagccaga attgaatccc cggcacgata cgtagacaag
     2761 tccacgccct tatgaatata ccattgtccg gtaaagggat ggcgattttg tccaaacgcc
     2821 atagacacgt gaccgatacc gcccctgatg ggccacaggc tcggaatatc agaaaacagc
     2881 gtactttgtg attctagaag cttgccaatt tcctgaatag gttgtactga attttcaaga
     2941 tatccagtta ggtgtttcaa ttctgctatt tcccgaactg agccctgggc ggtttcctta
     3001 acattgaaaa gggaagaaag atcgccgttt tggatattac tttttagggg ttgggcgtta
     3061 tccatgccga ttagagacag ggttccagaa aaagcagcct gaaaattctg tgctgccttg
     3121 aaaagattcc ctgtttcatc ttttagttca tcaagactcg cccgtgctgt tcgggcctct
     3181 tctcttaact ctgtcagatt ttgctcggat acaatcgctc gacgcgaaaa ccaaaaaaac
     3241 gagccaaaaa taccagtaag ggcaaccgca aagaaaagca gagaaaaaac agtggtttgg
     3301 aagtttatga ctcttttctg ggaatgggga acaaccatga ttgttagctt tcgctgtcca
     3361 ccctgggaga ttgaagagca gaccccgtgc acggacgaag ctcccttgca gagactacgc
     3421 ccaatggagc ggaatattgt attttctacg cgtttataac cacgtgtttt gggcacagca
     3481 atctccctta aagacaagca aaaaaccaac tttgccatgt ataataccat ggattaaaaa
     3541 aaactgtcaa atccgcaacc ccagtgaact gttactaaac cgtttttccg gttgacgccc
     3601 catccaacgt gtgttaccct tccctaaata ccccatatcc agtttggata tgttttccgc
     3661 tgtcccactg gacagatact acgcaacgat cggatgtcag ccatgcagtt ttttgatact
     3721 catgcccata tagggcttat atatgat
//
LOCUS       DAOQXQ010000074         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25397_length_3744_cov_2.322310, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000074 DAOQXQ010000000
VERSION     DAOQXQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25397_length_3744_cov_2.322310"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKH81_01955"
     CDS             complement(<1..1052)
                     /locus_tag="PKH81_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ96830.1"
                     /translation="MEILDDAKCNTATILVIDDELVIRDSFRDYLEDYGFTVRTAENG
                     KKGLELLEEQAIDIVMTDLRMPELDGLAVLRSCSEYNPDMPVIVVSGTGRIQDVVEAL
                     HVGAWDYLLKPIEDMTVLLHIVNKAVSRVEMLREGRQYKEHLEAEVIRRTAELEHTNQ
                     ELLLSRRQVIRILSQAAEYRDFETGNHFLRVAAFCACIARGLGWDDRDVSCIELASPV
                     HDIGKIGIPDNILMKPGPLESDEWVEMKRHCEYGNAILLGKKIPEFYFFNALSSGSDT
                     TQGCPMLEHAATIALSHHESWDGSGYPSGLQGEEIPIEARITAVADVYDAISSKRPYK
                     DPWPEEDCLAHMRKHSG"
     gene            1177..2265
                     /locus_tag="PKH81_01960"
     CDS             1177..2265
                     /locus_tag="PKH81_01960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ96831.1"
                     /translation="MKLQEIYHAELEKLRVVHNYRTFIILSIILFASCALFLAQHILL
                     PDTYSEPWIGQIYLYFYGISLAASLFFIVGLQIFRLARINWAVSATSLAFSLYCVTLG
                     LALTVLDYHTYQNLTATYISTIGVTVMLSAPIWAYIVIVIYLLGAFILVYTQYLASIS
                     NPSIFISIFFLAIISAVFGIYTEGVRTKGELARLQMKDLMLRDPLTGAYNRLFINEYI
                     AHSLQAKKRYHTDLSCLLLDVDFFKKINDRYGHQTGDLVLSELVATISRIVRSADIIA
                     RMGGEEFLIILPQTSLPSAIVVANKILTGINTVKLAGKEITVSIGIAEFNENEEFVSL
                     YHRIDEALYKAKTNGRNRAEVSMARQVV"
     gene            complement(2279..>3744)
                     /locus_tag="PKH81_01965"
     CDS             complement(2279..>3744)
                     /locus_tag="PKH81_01965"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ96832.1"
                     /translation="LKDVKPDILITDIKMPFMDGLALSRIVKKTQPWVKIIILSGHDE
                     FSYAKEAISIGVEDYLLKPCSASDMLAVLQKVAARIEAEKQEKSNTERLRQRIIGGTE
                     ILRQRWLCDLVSGSLSFEVAFEQAREWDLDLVSRHYAIAIVELNAEGGAGKSLERSRE
                     IIENLANASGEIIPFSFGVETLAFIIKGASLETVDESAYSLAQGIKFEVERNTPVRVA
                     VGIGSTVSRLAELPQSFSDAKTAVKYLSTTGKFKIIGINDIRAGLADGSASPGSDPIA
                     DRLRYTVRSDVECLVDQWSTLLGDNGSGNGITGYYLLYDIVFAASTVGQELGLVPADI
                     FPDGIDQERISLIAQSEKSFRDEVRRILFVLIDKREMRNGSGRENLIQRAKRYIDAHY
                     FDPDISLHVVASQVNVSPNHFSTVFSQEAGESFIEYLTCVRLSRAKQLLVSTSMKSAD
                     IAHEVGFSDPHYFSFLFKKHEGISPREFRSDKNEAEL"
BASE COUNT         1023 a          935 c          736 g         1050 t
ORIGIN      
        1 cccgaatgct ttcgcatatg cgcaagacaa tcctcctctg gccagggatc cttatacggt
       61 cttttactcg atatcgcatc atatacatcg gcaaccgcgg tgatgcgagc ctctatgggg
      121 atttcttccc cttgcaagcc cgaaggataa ccgctgccat cccaactttc atgatgactc
      181 aaggcaatcg tggcggcatg ttcaagcata ggacatccct gcgtggtatc ggagccggaa
      241 cttaaggcat taaaaaaata aaactcagga atttttttcc caagaaggat ggcattgcca
      301 tattcgcaat gtcgtttcat ctctacccat tcatcgctct caagcggacc gggcttcata
      361 agaatgttgt cgggaatacc aatcttgccg atatcgtgaa ccggggacgc aagctctata
      421 caggatacat cccgatcgtc ccagccaagc ccgcgagcga tacaggcgca aaaggcagca
      481 acacgcaaga aatgattgcc ggtttcaaaa tcgcgatact ctgccgcctg agaaagaatg
      541 cgaataactt gacggcggga cagtaagagt tcctgattgg tatgctcaag ttcagctgtt
      601 ctgcgaataa cctctgcctc aagatgttcc ttatattggc gtccttccct tagcatctct
      661 acacgggata cagccttgtt cacaatatgc aataagacgg tcatatcctc aatcggttta
      721 agaagataat cccaggcgcc aacatgcaag gcctccacta catcttgaat acgccccgta
      781 cccgacacaa caataacggg catatcagga ttatactcag aacatgagcg caataccgcc
      841 aagccatcta gctcgggcat tctcaaatcg gtcattacaa tatctatggc ttgttcttca
      901 agaagctcta accctttttt accgttttcg gcggtacgta ccgtaaaacc gtaatcctca
      961 aggtaatccc taaaactgtc gcgaattacc agttcatcat caatgacgag gatagtagct
     1021 gtgttacact ttgcatcatc caggatttcc atatagcaaa gtgtaggatg cgtacctctg
     1081 tttttccagc cttctgtccg ggaattactc ttttttgcgc aatcaccaat tttggagatt
     1141 tttgcatttt gcccgggtgt gtcgtatatt tttactgtga aattacaaga aatataccat
     1201 gcagaactcg aaaaactacg ggttgtgcat aattaccgaa cctttatcat attgtccatt
     1261 atcctctttg caagctgcgc cctgtttttg gcccaacaca tcctcttgcc cgacacctat
     1321 agtgaaccat ggattggtca gatatacctg tacttttacg gcatctcttt ggctgcatcc
     1381 cttttcttta ttgtaggctt acaaatattt cgccttgctc gcatcaattg ggcggtctcg
     1441 gccacatccc ttgcctttag cctctattgt gtaacattgg gcctcgccct taccgtgctt
     1501 gattatcata cctatcaaaa tcttacggcc acctatattt cgaccatcgg agtaacagta
     1561 atgctgagcg caccgatatg ggcgtatatt gttattgtca tctatctgtt gggcgctttt
     1621 attcttgtgt acactcaata ccttgcatct atttcaaatc caagtatttt tatttcaatt
     1681 ttctttttag ctattattag cgcggtattt ggaatatata ccgaaggagt acgcaccaag
     1741 ggggaactag cacgactaca aatgaaggac cttatgctcc gcgatccact cactggcgcg
     1801 tacaatcggt tatttataaa tgaatatata gcccacagcc ttcaggctaa aaaacggtat
     1861 cacaccgacc tttcttgtct cttgctcgat gtagattttt ttaaaaagat aaatgatcgc
     1921 tatggccatc aaaccggaga cctggttctc tctgagttag tcgcaaccat atcccgcatc
     1981 gtacgaagcg ccgatattat cgcccggatg ggcggcgaag agtttttgat aatacttccc
     2041 caaacatcgc ttccgtcggc aatagttgtt gccaacaaaa ttttgaccgg tattaatacc
     2101 gtcaaattgg caggaaaaga aattaccgta agtatcggga tagcggaatt taacgagaac
     2161 gaagagtttg taagcctata tcatcgtatc gacgaagcgc tgtataaggc aaaaacaaat
     2221 ggtcgaaatc gcgcagaggt ttcaatggcc cgtcaagttg tgtaataact tacaaaaact
     2281 atagctcagc ttcgttttta tccgatctga attctctcgg tgatattcct tcgtgcttct
     2341 tgaaaagaaa gctaaaatag tgcgggtcgc taaatcctac ctcatgggca atatccgcgc
     2401 tcttcatgga tgtagatacc agtaattgct tggcccgaga caagcgcaca caggttaaat
     2461 actcgataaa gctttctccc gcctcctgag aaaaaacggt actaaagtga ttcggactaa
     2521 cgtttacctg tgatgccaca acatgcaagg atatgtcggg gtcaaaataa tgggcgtcta
     2581 tatatcgctt ggccctttgt atcagatttt ccctgcccga tccatttctc atctcccgct
     2641 tatcaatgag tacaaaaagt attcttcgaa cctcatcacg aaacgatttt tcactttgag
     2701 ctataagcga tattcgctct tgatctattc catcagggaa gatatcagcc ggcactaatc
     2761 cgagctcctg accaaccgta gaagcggcaa atacaatatc gtagagcaaa tagtatccgg
     2821 taatgccatt gcccgatcca ttgtctccaa gaagcgttga ccattgatct acaagacatt
     2881 caacatccga tctaacggta tacctgagcc tgtctgcaat cggatctgat ccgggggatg
     2941 cacttccgtc ggccagtcca gctcgaatat catttatccc gattatcttg aacttcccgg
     3001 ttgtagataa atactttacc gccgtttttg cgtcggaaaa tgattggggc aattcagcta
     3061 gacgcgatac ggtcgatcct attccaacgg caacacgaac cggggtattt cgttcaacct
     3121 cgaatttaat accctgagcc aaagaatagg cactctcgtc taccgtttcc aaactggcac
     3181 ccttaattat aaatgcaagt gtttccacgc caaaagaaaa cgggattatc tctcctgagg
     3241 cattggccag attttcgatt atctctcgcg atcgctcaag agacttaccc gcaccccctt
     3301 ctgcgttaag ctcaacaatc gctatagcat aatgacgcga aaccaaatct agatcccatt
     3361 ctctcgcctg ttcaaaggcc acttcaaacg aaagagatcc actgactaaa tcacataacc
     3421 atcgctgtct tagaatttcc gtgccgccaa ttatacgctg acgcaatcgt tctgtattac
     3481 tcttctcctg tttttcggcc tctatacgtg cggctacctt ttgtaaaacg gctagcatat
     3541 ccgaagcaga acacggttta agaagataat cctctacgcc aatagatatc gcttcctttg
     3601 cataactaaa ctcatcatgg ccagagagaa taataatttt aacccagggc tgtgtttttt
     3661 ttacaattct ggaaagggcc aaaccatcca taaatggcat tttaatgtcg gtgatcaaaa
     3721 tatcgggttt aacatcctta agta
//
LOCUS       DAOQXQ010000075         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25435_length_3740_cov_3.504206, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000075 DAOQXQ010000000
VERSION     DAOQXQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25435_length_3740_cov_3.504206"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKH81_01970"
     CDS             <1..880
                     /locus_tag="PKH81_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96833.1"
                     /translation="LCLGWLAAVGTSFSFSSEPAYLATKFVSRNTLHLRLSPGVFADV
                     VVWTPDAETEVKGLVYYAGGPLPSSSLRPTMGAIMAENGFVCIAADYRSFRDWDNPLW
                     AYSGIRYVAAVFERATGNQGLLPSKAALDAVHSLDFFRFSRLASLVVPDVYRRIISER
                     SLPLYLVLEGSAVSPGLNDRQSTSLPFAGTICLGGDASSAVTESVRIALSTTLPALSA
                     SVPVMIVDELTLPGFGELTANDPLAARLMGLPRDALRKEAERTARRVVSWLLFRDSHL
                     ATDIQIVPQGVVDEGE"
     gene            867..1628
                     /locus_tag="PKH81_01975"
     CDS             867..1628
                     /locus_tag="PKH81_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ96834.1"
                     /translation="MKANELASYLDNALDSDCFASNDPSRNGLQVDGDGRELSKVAFA
                     VDACVETIERAAAAGADMLVVHHGLFWKDIELVVANHRKRLLALLSTGIALYASHLPL
                     DAHSELGNNAALADRLGIQDRQRFGQWKGSLIGWKGCLAEPVSLDKALSLVLSSGELP
                     LHVLPFGPERITSVGVVSGGGAAEMYQAIDEGLDLFVTGEIGHEEYHHALENGISVIA
                     AGHYQTETLGVRRLAAHLGSEIGMDTIFIDVPTGL"
     gene            1644..2207
                     /gene="lspA"
                     /locus_tag="PKH81_01980"
     CDS             1644..2207
                     /gene="lspA"
                     /locus_tag="PKH81_01980"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNQ96835.1"
                     /translation="MKGFMMNATHKERLIPFSLCLGIIALDQLTKLIVSLTVRPWSVG
                     ASFFDGILRIVRVYNPGIAFSIGGSLSGSVRGVLFALLPFIVIVIVTAVYFRSNDLTR
                     YQRWCVSGILGGGLGNIIDRFFRPEGVLDFIDVKFWGLFGLERWPTFNVADSAVVVCG
                     ILLMLSLLGSVMQEARGKDTDSAGEAL"
     gene            2204..2497
                     /locus_tag="PKH81_01985"
     CDS             2204..2497
                     /locus_tag="PKH81_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96836.1"
                     /translation="MKNKKANTVVFIVCGTIVNLLMALLIIVILFIAVSRIALIVGEQ
                     KAALLIPLAIIGGIIISMIIYQRLTAWVIERFGLADKLDPLFVSSKRKKKRLE"
     gene            complement(2518..>3740)
                     /locus_tag="PKH81_01990"
     CDS             complement(2518..>3740)
                     /locus_tag="PKH81_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96837.1"
                     /translation="RYALRFGLVLPTLLTPEQKDLFSRIIDKNLYGEPVYYIDEWLYA
                     IGSGKINPSSTDEAKTSRSDESSRFQQLLSKAQGKLQSAENLVRAKSEERVRSENELM
                     SRIGSIFEHEPLSTIPGAKAPFTEQQKRSISDLSESLRKIMALDKELASFVRDHGTAE
                     SDVRVLQQKVDSTGGGTTNASGISAEFDTIRQMAKMTCGRQGNHFPILTREFFHCSSR
                     EIGIRENVIETLRWIENIDPEAYCRTYKSQLNRIPPFVILIPSYGDYGLCWEPFDRYN
                     RVTSRGRIAIPMYGKNLLIAILTAVADLRWQVAKEKASYYWMEEGLTGNYYQWFQKQK
                     LKGDVKEYFINDYLLWMIKESEGIQKLDKEVRGVFWRYMPFSAEIKAKLKPRALVYQE
                     LCQRDLNREMSDGY"
BASE COUNT          798 a          827 c          970 g         1145 t
ORIGIN      
        1 cctgtgtcta ggctggttgg ctgcggtggg gacctctttt tcgttttctt cagagcctgc
       61 atatctggcc actaaatttg tctctcgtaa tacgctgcat cttcgcctgt ccccaggggt
      121 ctttgcagat gtagtggtgt ggacgccaga tgcagaaacc gaagttaagg gtttggtgta
      181 ttacgcagga ggcccgttgc catcctcctc tttgcgacct actatgggcg cgataatggc
      241 agaaaatggc tttgtttgta ttgccgctga ttatcgatca ttcagggatt gggacaaccc
      301 gttgtgggcc tattcaggta tccgatatgt ggcagcagtt tttgaacggg ctactggcaa
      361 ccaaggcttg ctgccatcca aggcggcgct tgatgccgtg cattctctgg attttttcag
      421 gttttcccgc cttgcctctc tggtagtccc tgatgtgtat cgtaggatta tctctgagcg
      481 ttcattgcct ctctatttgg ttttggaagg gagtgcggtt tccccgggtc ttaacgatcg
      541 ccagtcaacc agtcttcctt ttgcaggcac tatctgtctt ggaggagatg cgtcctctgc
      601 cgtaactgaa tccgttcgta ttgcattatc aactacattg cccgccctat cggcttcggt
      661 gccggtaatg atagtggacg agcttactct tccgggcttt ggcgaactta cggcgaatga
      721 tccgcttgct gcccgattga tgggactccc ccgtgatgct ttgcgcaagg aagcggagcg
      781 caccgcaagg cgcgtggttt cctggctctt gtttagggat tcacatctgg caactgatat
      841 tcagattgtt ccccaaggag tggttgatga aggcgaatga gcttgcttcg tatctggaca
      901 atgcccttga ctctgattgt tttgcttcaa atgacccttc caggaatggt ttacaggtag
      961 atggggatgg aagggagtta tccaaggtag cctttgcggt agatgcctgt gttgagacca
     1021 tagaacgtgc cgccgctgcc ggagcagata tgcttgtggt tcatcatggc cttttttgga
     1081 aggatattga gcttgtagtt gcgaatcatc gcaaacgtct tttagccctg ttatcaaccg
     1141 gaattgccct atatgctagc cacttgcccc tggatgctca ttcagaattg ggcaataacg
     1201 cagcacttgc agatcgttta ggtatacagg accgacagcg gtttgggcaa tggaagggct
     1261 ctctgattgg gtggaagggt tgtcttgccg agccggtttc tttggacaag gccctgtctc
     1321 ttgttctttc ttccggagag ctgcccttgc atgtgttgcc gtttggcccc gaacgaatta
     1381 cctctgttgg cgtagtatct ggtgggggcg cggcagagat gtaccaagcg attgacgaag
     1441 ggcttgatct gtttgttacc ggggaaattg gacacgagga ataccatcat gctctggaga
     1501 atggtatttc tgttatcgct gctggacact atcaaactga aacgcttgga gttcgccgtt
     1561 tggccgccca tcttggcagt gaaatcggga tggacaccat ttttatagac gtacctactg
     1621 gtttataggt agtatacaaa cgtatgaaag gattcatgat gaacgcaacg cacaaagaac
     1681 gcttaattcc gttttccctt tgtttgggaa ttatcgccct ggatcaatta actaaattaa
     1741 ttgtttcatt aacagttcgc ccctggtcgg taggagcttc cttctttgat ggaattctgc
     1801 gaattgtgcg tgtgtataac ccgggcatcg cctttagtat tggcggctct ctttccggat
     1861 ccgtcagagg cgtgttgttt gccttgctcc cctttatcgt cattgttatt gtaacggctg
     1921 tgtattttcg cagtaatgat ttgactcgct accaaaggtg gtgtgtgtcg ggaatccttg
     1981 gcggcgggct tgggaatatc attgatcgtt tttttagacc cgaaggcgtt cttgatttta
     2041 ttgacgtaaa attttggggt ttgtttggtc tggagagatg gcccacattt aatgtggccg
     2101 actcggctgt tgttgtgtgt gggattttgc ttatgctttc tttgcttggc tctgtaatgc
     2161 aagaggcacg gggaaaggat acagactctg cgggagaagc tttatgaaaa ataaaaaggc
     2221 aaacactgtt gtttttatcg tatgtggaac gatcgtaaat ttgttgatgg ccttgctcat
     2281 cattgtgatc ttgtttatcg cagtaagtcg tattgctctt atagtaggcg agcaaaaagc
     2341 agccctgttg attcccttgg caattattgg gggaattatt atttccatga ttatctatca
     2401 gcggcttacc gcttgggtta tagaacggtt tggtctggcc gacaagcttg atccgctttt
     2461 tgtatcgtca aaacggaaaa aaaagagact cgaataacga gtctcctgtt cgcatactta
     2521 atatccgtcg gacatttcgc gattcaagtc gcgctggcac aattcttgat acaccagggc
     2581 ccgcggcttg agctttgcct taatctctgc agagaaaggc atgtagcgcc agaacacccc
     2641 gcgaacttcc ttgtccagtt tttggattcc ttcgctttcc ttaatcatcc acaataaata
     2701 gtcgttgata aaatattctt ttacgtcgcc ttttagcttt tgtttttgga accattggta
     2761 gtagttgccg gtgagtcctt cttccatcca atagtaagac gctttttctt ttgctacctg
     2821 ccatctaaga tcggcaactg cggtcaggat ggcaatgagc aagttcttgc cgtacattgg
     2881 tatggcaatg cggccccggc tggttacgcg gttatagcgg tcaaagggct cccagcacaa
     2941 accgtaatct ccataggaag gaataagaat aacaaaaggc ggtatacgat taagttgaga
     3001 cttataagtc ctgcagtacg cttcaggatc gatgttctct atccatcgaa gggtttctat
     3061 gacattctcg cggattccga tttctctgct tgagcaatga aaaaactctc gtgttagaat
     3121 tggaaagtga ttgccctggc ggccgcaggt catcttggcc atttggcgta tcgtgtcaaa
     3181 ctctgcggat attcctgatg cgttggtggt tccgcctccg gtagaatcga ctttttgctg
     3241 cagaacgcgt acgtcggatt ctgccgttcc atgatcgcga acaaaagaag cgagttcctt
     3301 gtccagcgcc ataatctttc gtaaagattc cgataaatcg gaaatagagc gtttctgttg
     3361 ttcggtaaaa ggcgccttgg cccccgggat ggttgaaagg ggctcgtgtt cgaatattga
     3421 tccaattcga ctcatcaatt cgttttcgct acgaactcgc tcttctgatt tagcgcgaac
     3481 cagattttct gcgctttgta gttttccttg ggcttttgac aataattgct ggaaccggga
     3541 ggattcgtct gagcggctgg ttttggcttc gtctgtactt gaagggttta ttttaccgct
     3601 accgatggca tagagccatt catcgatgta gtacacgggc tccccataca ggtttttatc
     3661 gataatccgg gaaaaaagat ctttttgttc tggagttaac agcgtgggaa gtacaagccc
     3721 aaacctgagt gcgtaacgct
//
LOCUS       DAOQXQ010000076         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25503_length_3735_cov_2.771467, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000076 DAOQXQ010000000
VERSION     DAOQXQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25503_length_3735_cov_2.771467"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="PKH81_01995"
     CDS             complement(<1..1820)
                     /locus_tag="PKH81_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNQ96838.1"
                     /translation="MQFPFPTQRITCVAVPVSALKSTHSCGIGEFADLVPFAEFCKKS
                     ALSMIQLLPVNDTGTESSPYSALSAFALHPLYLSIRDIPEGAPYHHDIGEIKNRFESL
                     PRFDYRGLRLAKMDLLRRIFDDNEKSILKDPALQDWIIKNPWICEYAVFMNLKHRFCE
                     ASWKQWDKMRTPSHGEIQDRWNSPKRKADHLFYAWLQMRLDEQFSKAVSQCAALGIAI
                     KGDIPILMNEDSCDAWANPEFFRDDLRAGSPPDDMNQLGQNWGFPIYNWDNLRDTGFS
                     WWKERLSVASRYYHAYRIDHILGFFRIWSIPSGNSTGYLGWTTPHAAITTPELHGLGF
                     SGDRLRWITEPHIPTRAIEAVNHFDYLGSHGLLSKVCNRIGNEELWLFKPTITGERDI
                     EKANLPQPVIAALLSAWKDRLLQETGRDDKGRALYSPIWNWKDTSAWKSLSSEEKEAM
                     GALFERKKAEGEQLWEAQALEILGELTRSCDMLPCAEDLGSIPQCVPSVLSQLGILSL
                     KVVRWERIWEQEGQPLRDLGSYPSLSVAASSIHDSSTLRMWWDSEGGREAFFAAYGSE
                     GFTQDYCESTASAVLERIAQAGSRIVSIPLQDFLALSSDTT"
     gene            complement(1878..2807)
                     /locus_tag="PKH81_02000"
     CDS             complement(1878..2807)
                     /locus_tag="PKH81_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ96839.1"
                     /translation="MEKEYESCTICPRSCAVNRNRGETGFCGETASLRLSWAGLHFGE
                     EPPVTGKNGSGTIFVTGCNLRCNFCQNFQISQEGMGAPVSLSTFTRICTALAEAGAEN
                     LNIVTGSHTIPALATALREARSCGVTLPVYWNSSAYETIEALELLQDVVTGWLPDLKT
                     LNPLFSASVFQAKDYPSTAKKALLWMAHRNQRVIVRHLALPGRKEDTRIVLDWFSRKL
                     KGKALLSLMTQYTPVSAHPQNHELTEFQNRLLTEQESKELVLLLDEYGIDNGFFQELV
                     NDTEWLPDFIRTQPFSSSLARPVWHWKEGFIRS"
     gene            complement(3180..3569)
                     /locus_tag="PKH81_02005"
     CDS             complement(3180..3569)
                     /locus_tag="PKH81_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96840.1"
                     /translation="MTNLDKFCSDAQRLVARAEKSPIDEKNFPNELLRLFASWSRDFD
                     PVASALASYWQDRYGRELSDSTKRKDAVEWFIALLSLLSGDFPATAHFADDDWDEIRE
                     TVSAEAETMDMDVVTAIMSILVERGKA"
     gene            3627..>3735
                     /locus_tag="PKH81_02010"
     CDS             3627..>3735
                     /locus_tag="PKH81_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNQ96841.1"
                     /translation="MNLKTVLTTDTVNLHLKGTSKEAIIDELLDILVKAG"
BASE COUNT          922 a          904 c          952 g          957 t
ORIGIN      
        1 gttgtgtcgg aggagagcgc aagaaagtct tgaagtggaa ttgacactat gcgggatcct
       61 gcctgggcaa tgcgttcaag gacggcactc gcggttgatt cgcagtagtc ctgggtaaac
      121 ccctcgcttc cgtatgctgc aaaaaaggcc tctctgcccc cttcgctatc ccaccacatc
      181 ctgagtgtag aactgtcgtg tatggagctt gctgccaccg aaaggcttgg ataagagcca
      241 aggtccctaa ggggctgtcc ctcttgttcc catatacgtt cccagcgaac aaccttgagc
      301 gacaatattc ctaattgcga aagaaccgaa ggtacacact gaggaatact gccaagatct
      361 tcggcacagg ggagcatatc gcagcttctg gtaagttcac caagaatctc cagggcctgt
      421 gcttcccata gctgctcccc ctctgccttt ttgcgttcaa acagggcgcc cattgcttcc
      481 ttttcctcac tggaaaggga tttccatgcg ctggtgtctt tccagttcca aatcggggag
      541 tacagggctc tgcctttgtc atcccgtccg gtttcttgta agaggcggtc cttccatgca
      601 gagagcaagg ctgcaattac cggttgggga aggtttgctt tttctatatc acgttcgcct
      661 gttatggtag gcttaaaaag ccagagttct tcattcccta ttctgttaca cacttttgac
      721 aaaaggccat gcgagccaag gtaatcaaag tgattaaccg cttctatggc tcttgtcggg
      781 atatgcggct cagtaatcca acgcagacga tcgccggaaa agccaaggcc atgtagctct
      841 ggagtggtta ttgccgcgtg tggagttgtc catcccagat atccagttga gttgcccgat
      901 ggaatagacc atattctaaa aaaacccagg atgtgatcaa tacggtaggc gtgataataa
      961 cgggatgcaa ccgaaaggcg ttctttccac caggagaagc cggtatccct aagattatcc
     1021 cagttatata tggggaagcc ccaattttgt ccaagttgat tcatgtcgtc tggagggctg
     1081 ccggctcgga ggtcatcccg aaaaaactcg ggatttgccc aggcatcgca ggagtcttcg
     1141 ttcatcagga tggggatgtc tccctttatg gcaatgccaa gcgctgcgca ttgcgacacc
     1201 gctttggaaa actgttcgtc cagtcgcatt tggagccagg cgtaaaacag atgatctgct
     1261 tttcgcttag ggctgttcca tcgatcctgt atctctccat gggagggggt tcgcatcttg
     1321 tcccattgtt tccaggatgc ctcgcaaaat cgatgcttca ggttcataaa aaccgcgtat
     1381 tcgcatatcc acggattttt tatgatccag tcctgcaggg ccgggtcttt caggattgat
     1441 ttttcattat cgtcaaagat tcggcgcaat agatccatct tagcaagcct gaggcctcgg
     1501 tagtcaaagc ggggaaggga ctcaaatcga tttttaatct ccccaatgtc atgatgatac
     1561 ggggctcctt cgggtatatc gcgaatgctc agatagagcg gatgcagggc aaaggcggaa
     1621 agggcgctgt acggggaact ttcggttccg gtatcattta ccggtaacag ttgaatcatg
     1681 gagagtgcgg attttttgca aaattctgca aagggaacaa ggtcggcaaa ctcaccgatg
     1741 ccgcaactat gcgtactctt gagcgcgctt acgggaaccg cgacgcaggt aattctttgt
     1801 gtggggaagg gaaattgcat acctccagta tacggtacgc tttaagaaaa aggaagtaaa
     1861 gataatgagt agcggcccta tgagcgtata aagccctctt tccagtgcca aacaggccgg
     1921 gcaagggatg aggaaaaggg ttgggttcgg ataaaatcgg ggagccattc tgtgtcattc
     1981 accaattcct gaaaaaaccc gttatcaatt ccgtactcgt caagtagaag aaccagttct
     2041 ttgctttctt gctcggttaa taaccgattt tgaaactcgg ttagttcatg gttttgggga
     2101 tgtgcggaca cgggcgtgta ttgggtcatc aaggatagaa gggcctttcc cttaagcttt
     2161 ctggaaaacc agtccagaac tatacgggta tcttcttttc ggcctggcag ggcaagatgg
     2221 cgaacaataa ctcgttggtt acgatgtgcc atccagagca aggccttttt cgcagtagac
     2281 ggataatcct ttgcctgaaa aacacttgcc gagaacagtg gattaagggt tttaagatct
     2341 ggtagccagc cggtaaccac atcctgcaac agctccagcg cttcaatagt ctcgtatgcc
     2401 gaagaattcc agtagaccgg gagggtaaca ccgcacgagc gggcttcgcg taaggcggtc
     2461 gcaagggcag ggattgtatg actgccagtg actatgttga ggttttctgc gccagcctct
     2521 gcaagcgctg tacagatccg ggtaaacgtg gaaaggctaa caggcgcgcc cattccttct
     2581 tgtgaaatct ggaagttttg acagaagtta cagcggagat tgcatcccgt aacgaagatt
     2641 gttcccgatc cgttttttcc ggtaaccggg ggttcttcgc cgaaatgaag accagcccat
     2701 gaaagccgaa gacttgcggt ttctccgcaa aaacccgttt cgccgcgatt tcggtttact
     2761 gcgcaggagc ggggacaaat agtgcaagac tcatactctt tttccatggg aagccgagta
     2821 tatcagtcag gaataaaaaa aaacaagccg gtctgtcacc ggcttgttgg agccttaaac
     2881 cccgctatgg gagtcgttcc ctgacgggaa cttcggcctc tcttattgaa cctgccatgt
     2941 agagcggaca ggttgacgat catctctgtg cgccggtttc tctccggcgt gcatcttcag
     3001 gcggattgtc catacagggc ctcccgtaac acatccgttg ccttgtgtac gaacggcttc
     3061 ttccgttgcc cgtccgtcac cctgatatcc gtgcagtaat aataaccgct tacatcccaa
     3121 atatcgttca tcacggctca gctgtcaaga aaaattgcac aaaaaacaca atctgtttgt
     3181 caggcttttc cacgctcaac aaggatagac ataatggctg taactacatc catgtccatg
     3241 gtttctgcct cggcggatac cgtttcacgg atttcatccc agtcgtcatc ggcaaaatgt
     3301 gctgttgcag ggaagtctcc cgaaaggagg gacagcagag caataaacca ttcaacggca
     3361 tcttttcttt ttgtagaatc ggaaagctct cttccgtagc ggtcttgcca ataggaggca
     3421 agggcagaag ctaccgggtc gaagtctctg gaccaggaag caaagagcct gagcaattca
     3481 ttggggaagt ttttttcgtc aatcggactt ttttccgcgc gtgcgacaag gcgttgtgcg
     3541 tcggaacaga atttgtctag gtttgtcatt agtttattgt atcaaaaaac cttgactttt
     3601 cctatgaagg aacgtaaact gaccctatga acctgaaaac tgtattgacc actgataccg
     3661 tcaatcttca tctgaaggga acctctaaag aggctatcat cgacgaatta ctcgacattc
     3721 ttgttaaagc cggaa
//
LOCUS       DAOQXQ010000077         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25609_length_3725_cov_2.223161, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000077 DAOQXQ010000000
VERSION     DAOQXQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25609_length_3725_cov_2.223161"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKH81_02015"
     CDS             <1..972
                     /locus_tag="PKH81_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96842.1"
                     /translation="TYEEKTGGVPGVDYGKELTATAKGTITTTSVPLIVMRSTSALLT
                     PLASAAIPSYIQTYEPLIFGDDSMLLFKGLYTADPFRAHAYMIASHTWKKIIPDNFDF
                     TGTCGTTLCYIKNDQILTSEDNGASWTSASVPVPPLPDHNPGLGAFVGENIALFYKGD
                     PSILLVSSDKGLSWTVHELPFSSVQNIVIHGNSIYIEDYSTIKKSNDFGSTWVTDTEL
                     SGSLRSSGTILYLDTKDGLFRLTNEEWVPFILPSDESYPSFHASAEDFIYTNAYRTGF
                     GLYIVSAGKGAWYFLSKDPEDCSYIRILGNTIAFIYKDRIDMYRINR"
     gene            complement(1026..2879)
                     /gene="guaA"
                     /locus_tag="PKH81_02020"
     CDS             complement(1026..2879)
                     /gene="guaA"
                     /locus_tag="PKH81_02020"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ96843.1"
                     /translation="MKNDTIVVLDFGSQYAHLIAKRLRLMGFYSEIALPSSDLSVFDG
                     AKGIIFSGGPSSVYADTVPEFNKEILSLTIPLLGLCYGHQLLALESGGKVGKAAVGEF
                     GIASLEKTDTGESELFTGIPFPAQVWMSHQDAVLSPPPGYRVIATTKDCPYAGLENLA
                     QKRWSLQCHVEVKDTPQGDTILANFASICGMEKNWSGERVLETIMADIQSQANRDGKE
                     RKVLLFLSGGVDSTVAFALLNKALGQERVLGLHIDNGFMRKDESAIIARRYRDAGFTN
                     FIVEDASDTFLAAVKGLADPQLKRKAVGETFITVRNQVVSRLGLNEEDWLLGQGTLYP
                     DIIESGGTKNSHTIKTHHNRVDGIQALIEKGLIIEPLRDLYKDEVRTLGKRVGLSDEL
                     VFRHPFPGPGLSINVLCRAPDLDDREELERARESIKAIDIDGELTNLGATVDALPVRS
                     VGVQGDFRTYRFPAVLSFKDIFSHFPSWDLLEKESSRITNASDEVNRTVLELAKKAGT
                     FSIREGWCDKARLDQTREVDAITLEAFKSAGWYTKIFQHLTINIPWSTTEGHCSIVLR
                     PVVSEDVMTARFAHLDRELLKSIVEKIMNLGYIDAVYYDITNKPPATFGWE"
     gene            complement(2951..>3725)
                     /locus_tag="PKH81_02025"
     CDS             complement(2951..>3725)
                     /locus_tag="PKH81_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ96844.1"
                     /translation="TADTLAAMRMAKKGGARIIAITNVVGSTLAREADLVMYTWAGPE
                     IAVASTKAFTTQLMCIYLIALQFGSLRGVLSEKDLEEHIGALEKIADQAEKLLSNKEE
                     IQRFASRNFNKTKAFFMGRLFDYAISLESALKLKEISYTHSEAFAAGELKHGPIALVD
                     EATLVVAICTQTALFDKMDSNIKEVKARGATALVITYDDETYFDKTADEVFRIPRTLD
                     PLTPIVSVIPCQLFAYYCSVQRGLDPDKPRNLAKSVTVE"
BASE COUNT          899 a          894 c          955 g          977 t
ORIGIN      
        1 acatatgaag aaaaaacagg cggcgttcct ggtgtagact atgggaaaga attaaccgcg
       61 actgccaagg gaacaattac gacaacttct gtaccgctaa tcgtcatgcg ctcaacgtct
      121 gccttgctga ctcccttagc ttcagcggcc ataccttcgt atattcaaac gtatgaaccg
      181 ttgatatttg gcgatgattc gatgcttctt tttaagggat tatataccgc agatcccttt
      241 agagcgcatg cgtatatgat cgcgtcgcat acctggaaaa aaataattcc ggacaatttt
      301 gattttacag gtacatgcgg cacaacactg tgctatataa aaaacgatca aatactcacc
      361 tcggaggata acggcgcttc gtggacatcc gcaagtgtgc ctgttccgcc gctacctgat
      421 cataatccgg gtttaggcgc ctttgtggga gagaacattg ctctttttta taagggcgat
      481 ccatcgattc tccttgtatc ttcagataag ggtctttcct ggactgtaca tgagcttccc
      541 ttttcatcgg tacaaaacat tgtgatccat ggcaactcga tatatataga ggactattcc
      601 acaataaaga agagtaacga ctttggttcc acgtgggtaa cagatactga attgagtggt
      661 tcgctccgtt caagcgggac catattgtat cttgatacaa aagatgggct tttccgatta
      721 actaatgagg agtgggtgcc gtttatcctt ccttctgatg aatcctatcc aagtttccat
      781 gcttctgccg aggattttat ctatacgaac gcgtatagaa ctggattcgg tctatacata
      841 gtttccgccg ggaaaggcgc ttggtatttc ttgagcaagg acccagaaga ctgttcctat
      901 atcagaatac tcggcaacac tattgcattt atttataaag acaggattga tatgtatcga
      961 attaatcggt aattttgaca catacactgt cctcgcctaa gggatgtaca cctcccgctt
     1021 gtcttttact cccagccaaa ggtcgccggc ggtttattgg taatgtcgta atagaccgca
     1081 tcgatatacc caaggttcat aattttttct acgatggact tgagcagctc tctgtccaga
     1141 tgggcaaagc gcgcggtcat gacgtcttcg gacaccaccg ggcgaagcac gatggagcaa
     1201 tgtccttctg tggtggacca gggaatgttg atcgttagat gttggaatat cttggtgtac
     1261 cagccggcgc ttttaaaggc ctcaagggta atagcgtcca cctcgcgggt ttggtcaagg
     1321 cgagccttgt cgcaccaacc ttcgcggatc gagaatgtac ccgctttttt cgccagttca
     1381 aggacggtcc tgtttacctc gtcgctcgcg ttggttatcc tgctcgactc tttttccaaa
     1441 agatcccagg agggaaagtg agaaaagata tccttaaaag aaagaacggc agggaatcga
     1501 taggtgcgga aatctccctg tacaccgact gaacgaacgg ggagggcgtc gaccgttgca
     1561 cccaagttgg taagctcccc gtcaatgtct atcgccttga ttgactcgcg cgctctctca
     1621 agctcttccc gatcgtccag atcgggggcg cggcacagga cgttgatgga aagaccgggg
     1681 ccggggaagg gatggcggaa gacgagctcg tcggaaagcc cgactctctt acccagggtg
     1741 cgaacttcat ccttgtataa atcgcgaagc ggttcaatga tgagtccttt ttcgattaag
     1801 gcttggattc cgtccacgcg gttatgatgg gttttaatag tgtgagagtt tttcgtacca
     1861 cccgattcaa taatgtccgg gtacagggtt ccctgaccaa ggagccagtc ttcctcgtta
     1921 aggccaagcc ggcttaccac ctgattgcgc acggtgataa aggtttcgcc aactgccttg
     1981 cgtttgagct gcggatcggc caggcccttt accgcggcaa ggaaggtgtc gcttgcgtct
     2041 tctactataa aattagtaaa gcccgcgtcg cgataccgcc tagcgatgat ggcgctttcg
     2101 tccttacgca taaagccgtt gtctatatga aggccaagca cccgctcctg gccaagggcc
     2161 ttattaagaa gggcaaaggc aacggttgag tcaacaccgc ctgaaaggaa gagtaagacc
     2221 ttgcgctctt taccgtcgcg gtttgcctgg ctttgaatgt ccgccatgat agtttccaac
     2281 acccgttcgc cgctccagtt tttttccatg ccgcagatgg aggcaaagtt tgcaagaatt
     2341 gtgtctccct gtggggtgtc tttaacctct acgtggcact gaaggctcca gcgtttttgt
     2401 gcaaggtttt ccagtcccgc ataggggcag tcttttgtcg tggcgataac gcggtatccg
     2461 ggaggcgggg agagaacggc gtcctgatgg ctcatccaca cctgagctgg aaagggaatc
     2521 ccggtaaaaa gctctgattc gcctgtgtcg gttttttcaa ggcttgcgat accaaactct
     2581 ccaactgctg ccttgccaac cttgccacct gattccagag caaggagttg atgtccgtag
     2641 cagagtccca aaaggggaat ggtaagggaa agaatttctt tgttaaattc gggaacggta
     2701 tctgcataga cggaagaagg gccgcctgaa aaaatgatac cctttgcgcc gtcaaagacg
     2761 gacaggtcgg aagagggtag ggctatctct gaataaaagc ccatcaaacg caaccgcttg
     2821 gcaattaaat gcgcgtattg gctgccaaaa tcaagcacta caattgtatc gttcttcata
     2881 gctcaatata gcaaaagcgg gcgcctttgt catgatgtaa gtactgctcg caggattgtt
     2941 ggtatttccc ttactccacc gtaacgcttt ttgccaggtt tcgcggttta tccggatcaa
     3001 gtccgcgctg aaccgaacaa taatatgcaa agagctgaca gggtattacc gagacgatcg
     3061 gggttaatgg atcgagtgtg cgtggaatgc ggaatacctc gtcagcggtc ttgtcaaagt
     3121 aggtttcgtc atcgtaggta ataaccaggg cggtagctcc gcgcgccttt acctctttaa
     3181 tgttcgagtc catcttgtcg aaaagggcgg tttgggtgca aatagccact accagggttg
     3241 cttcgtctac cagggcaata ggtccgtgct taagttcccc ggcggcaaag gcctcggaat
     3301 gggtatagct gatttcctta agcttaagag cgctttccaa actgattgca taatcaaaga
     3361 gccgtcccat aaagaaggcc ttggtcttgt taaagttccg gcttgcaaat cgctgaattt
     3421 cttctttgtt ggaaaggagc ttttctgcct gatcggcgat tttttccagg gcgccaatgt
     3481 gttcttctaa atccttttcg cttaagactc cgcgcaggga gccgaattgc aaggcgataa
     3541 ggtagataca cataagttgg gtcgtaaatg ccttggtgga ggcaacggcg atctccggtc
     3601 ccgcccaggt atacataacc agatctgcct cgcgggcaag ggtggatcct accacgttgg
     3661 ttatagcgat aatacgggcc ccgccttttt tagccatacg catggcggca agggtatcgg
     3721 cggtc
//
LOCUS       DAOQXQ010000078         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25644_length_3721_cov_2.924986, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000078 DAOQXQ010000000
VERSION     DAOQXQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25644_length_3721_cov_2.924986"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKH81_02030"
     CDS             complement(<1..224)
                     /locus_tag="PKH81_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HNQ96845.1"
                     /translation="MKSKQESAVALGKLLAEHNGIDVKVLDVSGSNSWADFFIIATVT
                     SAVHSRGLQRHVHESLKALSLEIRPTRKKAS"
     gene            complement(241..1434)
                     /locus_tag="PKH81_02035"
     CDS             complement(241..1434)
                     /locus_tag="PKH81_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNQ96846.1"
                     /translation="MRGFRIDRSIIFLIIILAILVIAAVSLVLGMKTNPVKESLSGDK
                     ILKVLLVLESEGKPLATNIIAYYPGNKRSAMFDIPGETGLILRSLERVDRIDAVYTEK
                     GIHAYRTEIEGLTGISIPFSIEVSLDNFSRLADLLSGLSVFIPSPVDIQDEQGRVLLP
                     SGAVTLDGDKLRTYVTYIDPLDQEGEASSRRQKALLAFFRALNDNFQYAFSDSLFPTI
                     EKSIRANISSVGIRMVLEELSQTDAERLVPQRVTGSLREVDGKLLLFPFYDGQLLKDI
                     IRQTLAGLTSENAAAQERIYALEILNGTGSHGLARNTSELYQSFGYDVLRIGNAETTE
                     TDQTVIIDRIGNQAVAQTVAQVIQCSNIVSTSVTDTGDDLYGTEAVVDFTIILGKDFN
                     GRYVR"
     assembly_gap    1563..1572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1573..2038)
                     /gene="yqeK"
                     /locus_tag="PKH81_02040"
     CDS             complement(<1573..2038)
                     /gene="yqeK"
                     /locus_tag="PKH81_02040"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNQ96847.1"
                     /translation="MTSDELMRITALVDQYAIKVLPTPRYLHSFRVATLARELAIRFG
                     IDEQEAFLAGLAHDICKAEGSDRLFSLVRSDGYGIGDIEAKKPSLLHGRAAAVLLERD
                     FSVDSVSILDAVRHHTFGSPSFDSLGKIVYVADKLEPGRQDIDPLHRTTALEK"
     gene            complement(2025..2606)
                     /gene="nadD"
                     /locus_tag="PKH81_02045"
     CDS             complement(2025..2606)
                     /gene="nadD"
                     /locus_tag="PKH81_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNQ96848.1"
                     /translation="MKLAVLGGSFNPVHNGHLALAEAAHREAGFDRVLLVPARFPPHK
                     QAPQEVCDAQRLHMLTLACPASSWLEVSSIELDREGVSYTIDTLRALRAQLHDLLTAK
                     IAMIIGEDLVTGFSSWKEAAALACEFDILLACRPGFPDHSFPFPHRRLENEPIAVSSS
                     AIRQAIREGRPWDSLVPPAVYRYIVEQRLYDER"
     gene            complement(2603..>3721)
                     /gene="obgE"
                     /locus_tag="PKH81_02050"
     CDS             complement(2603..>3721)
                     /gene="obgE"
                     /locus_tag="PKH81_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNQ96849.1"
                     /translation="VSSGKGGNGCASFRREKYIPMGGPSGGDGGKGGDLIFEIRRNLR
                     TLAHLRYKQSFKAKNGGDGAGNKCYGKDGEDCIVPLPPGTLIRDADTGDLIHDFGFDM
                     EGQLLLLKGGNGGWGNVHFKSSTNQAPRTALKGQEGQTRRLRIELNIIADIGFVGFPN
                     AGKSSLLDFYTNARPKIAPYPFTTKIPNLGVLRVNEEQDIILADIPGIIEGASEGHGL
                     GIRFLKHIARSAGLAFIIDLSDDDFLDSYEKLETELASFSEELINKERIVLANKLDLP
                     EARERLEELRARYPNLDILGISVHNRWGLDEVASAFVTMVNKTAQAEALAAGVSEDIP
                     PGPDFMHAELEDPLYKETDTEDFGATVSLNRKRKGKKK"
BASE COUNT          968 a          994 c          847 g          902 t
ORIGIN      
        1 gaggccttct ttcgggttgg tctaatctcc aaagaaaggg ccttaaggga ttcatgcacg
       61 tgacgttgca agcccctgga atgaacggcg ctggtaaccg tagcgataat aaaaaaatcc
      121 gcccaggaat tgcttcctga tacatccaga accttaacgt ctataccatt atgctctgca
      181 agcagctttc ccaatgcgac tgcactctcc tgtttactct tcatgctttc catatctctc
      241 ctatcggaca tatcttccgt taaaatcctt gcctaaaata atggtaaaat ccactaccgc
      301 ttctgttccg tataagtcat ccccggtatc cgtaaccgag gtgcttacaa tattggagca
      361 ctgaataacc tgggcaacgg tctgggcaac cgcctgatta ccaattcgat cgatgataac
      421 cgtttgatct gtttccgtcg tttcggcatt cccgattctc aatacatcat agccaaaact
      481 ttgatagagc tccgaggtat tgcgggccag gccatgactt ccggtaccgt taagaatctc
      541 aagggcgtat atgcgctcct gggccgcggc gttttcggag gttaaccccg ccagggtttg
      601 ccggataata tccttaagta gctgtccgtc ataaaatgga aataacagga gctttccgtc
      661 cacctcccgc agcgagccgg taacccgttg aggcaccaag cgttccgcat cggtttggga
      721 caattcctct aacaccatcc ggatacctac gcttgatata ttggctcgta tcgatttttc
      781 tatggttgga aacaaggaat cggaaaacgc atactgaaag ttatcattca aagcgcgaaa
      841 aaaggcgagc agggcttttt gccttctgct ggaagcctct ccctcctgat caagtggatc
      901 aatataggta acataggttc gcagtttatc tccgtccagg gttaccgccc ccgacggaag
      961 aagcacccgc ccctgttcat cctgaatatc aacaggggaa ggaataaaca cgcttaaccc
     1021 ggacaataaa tcggcaagtc gcgaaaaatt atccagcgag acctcgatgg aaaagggaat
     1081 ggaaattccg gttagccctt cgatctcggt tctataggca tgtatcccct tttcggtata
     1141 taccgcgtca attctatcca cccgctctag actgcgaaga atcaagcctg tttcgcccgg
     1201 gatatcaaac atagcagagc gcttattacc cggataatag gcaataatat ttgttgccag
     1261 gggttttccc tcgctttcca acacgagtaa gacctttaaa atcttgtcgc cggacaaaga
     1321 ttccttaacc ggattcgtct tcatccccaa caccagagat accgcggcaa tcaccaaaat
     1381 ggcaaggatg ataataagaa aaataatact acgatcgatg cggaaacctc tcatgccgtt
     1441 tctccccttt ctccaagaac cgtgagcatc tccatcgtct ctggagatat ctcgtgaccc
     1501 ctgtgttcta gatagcgaac gccctcctgg gcaaccattc gtgtcattcc gtccaaatcc
     1561 ttnnnnnnnn nnccttctcc agggctgttg tgcggtgaag cggatcaata tcttgccgcc
     1621 caggttccaa tttatcggct acatacacaa tcttcccaag agaatcaaag gatggagagc
     1681 cgaaggtatg atgccggacg gcatcgagta tagacacaga gtctacagaa aaatctcgtt
     1741 caagcaaaac cgcggcagcc cttccatgca acaaagaggg cttttttgct tcaatatcgc
     1801 caataccata tccgtcgctg cgaacaagcg aaaacaaacg gtctgaccct tcggccttac
     1861 agatatcatg agcaagaccg gcaagaaagg cttcctgttc gtcaattcca aaacgtatgg
     1921 ccaattccct ggcaagagtc gctaccctga aggaatggag ataccgggga gttggtaaca
     1981 cttttatggc atactggtcc accaaagcgg taatgcgcat caattcatcg ctcgtcatac
     2041 agcctctgct ctacaatata ccggtatacc gcagggggaa caagagaatc ccatgggcga
     2101 ccttcccgaa ttgcctggcg aatagccgaa gaagaaacag caatcggctc gttttccagg
     2161 cgtctgtgcg gaaagggaaa gctgtgatcg ggaaacccgg gccggcacgc cagcaagata
     2221 tcgaactcgc aggccaaggc agcggcttcc ttccaggagc taaaccccgt aaccaaatcc
     2281 tctccgataa tcatggctat ctttgcggta agaaggtcgt gtaactgagc acgcaaggca
     2341 cgcaaggtgt cgatggtata cgacacccct tcgcgatcaa gttcaataga cgaaacctct
     2401 agccacgaag aagccgggca cgccagtgta agcatatgaa gtctttgagc atcacagacc
     2461 tcttgcgggg cctgtttatg cggcggaaat cgggcgggaa ccagcaagac ccggtcaaat
     2521 ccggcctctc gatgagccgc ttccgcaagg gcaaggtggc cgttatgaac gggattaaag
     2581 ctccctccaa gaacggcaag cttcatttct tcttgccctt tcgctttctg ttcaagctga
     2641 ctgtggctcc aaaatcttct gtatcggttt ctttatacag aggatcttca agttccgcat
     2701 gcataaaatc cgggcctgga ggaatgtctt cagacacacc ggcggcaagg gcctctgcct
     2761 gtgcggtttt attgaccatc gttacaaagg ccgaggcaac ctcatcaaga ccccagcggt
     2821 tatgaacgga aatgccgagt atatccagat tcggataccg ggccctgagt tcctcaagac
     2881 gttccctggc ttcgggaaga tcaagcttgt tagccagcac tatccgctct ttgttgatca
     2941 attcctccga aaaggaagca agctctgttt ccagtttttc gtaggagtcc aaaaagtcgt
     3001 catcggagag atcgataata aaggcaagac ctgctgaccg ggcgatatgc ttcaggaagc
     3061 gtatgccaag accatgacct tcacttgcac cttcgataat gccggggatg tcggccagga
     3121 taatatcctg ttcttcgttt acccgtagta ccccaagatt aggaatcttt gtggtaaacg
     3181 gatacggtgc tatcttggga cgtgcattgg tataaaaatc gagaagagac gattttcctg
     3241 cattgggaaa gcccacaaat ccaatatcgg caatgatatt caattcgatg cgaagacgcc
     3301 tcgtctgtcc ttcctgaccc ttaagggctg ttcggggggc ttgattggta gaactcttga
     3361 aatgaacgtt tccccaaccg ccattgcctc ccttgagtag cagcaactgg ccttccatat
     3421 caaagccaaa gtcatgtatc aaatcgccag tatccgcgtc gcgtataagc gttcccggcg
     3481 gaagggggac aatacaatct tctccgtctt taccgtaaca tttatttccg gcgccgtctc
     3541 cgccattttt tgccttaaaa ctctgcttat atctaagatg ggccaaggtt ctaagatttc
     3601 tcctgatttc aaaaatcaga tctccaccct taccgccgtc tccgccggaa ggtccgccca
     3661 tagggatata tttctcccgt ctaaaagaag cgcaaccatt accgccctta cctgaggaga
     3721 c
//
LOCUS       DAOQXQ010000079         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25692_length_3715_cov_3.622951, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000079 DAOQXQ010000000
VERSION     DAOQXQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25692_length_3715_cov_3.622951"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKH81_02055"
     CDS             <1..237
                     /locus_tag="PKH81_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNQ96850.1"
                     /translation="LEKAATRAGVAVIKKPIRGGTDGSRLTEMGIPTPNIFTGGHNYH
                     SRSEWASIEQMGKTVETLIQLAIIWGEDHVQVQN"
     gene            218..1501
                     /gene="murA"
                     /locus_tag="PKH81_02060"
     CDS             218..1501
                     /gene="murA"
                     /locus_tag="PKH81_02060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ96851.1"
                     /translation="MYKYKIEGGFPIKGTIKASGNKNAALPCIAAALLTDKPVILRNL
                     PEIEDVQVMLDVFCALGGSVETLQDGAKRLSIASLSRTDIPQDLSRKIRASILFAGPL
                     LARAGKAVLPPPGGDVIGRRRLDTHFLALTELGARVEIDGQFTFTANKLKGQDIFLDE
                     ASVTATENAVMAAVLAEGQTILTNAASEPHVQDLCAMLNSMGASISGIGSNILTIEGV
                     QNLSGTDFRIGSDYMEIGSFIGLAAVTHGSITITDVQPRDLRPVRLAFGKLGIRWDIE
                     GTTLTVPSQQSLQINSDLGGMIPKIDDAPWPGFPPDLTSIMTVVATQVEGTVLIHEKM
                     FESRMFFVDKLIGMGARITLCDPHRAVVSGPSVLHGSELVSPDVRAGMAMVIAALCAR
                     GESVISNVYQIERGYENLTTRLVSLGAHIERISSC"
     gene            complement(1535..3316)
                     /locus_tag="PKH81_02065"
     CDS             complement(1535..3316)
                     /locus_tag="PKH81_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96852.1"
                     /translation="MVELKRITPIVIAHLLIGLAICVVYSLVSTLDISLLPPFITPYR
                     IKSSILLFIQYFPSLQVSGILVGYALAFSKVSDLKVERWSEGLFSCLKDAFALSLFLL
                     CIYVILAEGLAPSLRTRNETAITRTTDYREYVSVARSLMVKDDHGRADGYLVAAEGIW
                     KDSEEVADLRESCRLRIAEKQGEGLSRQSRLPDPLIIADATGLTVLGALDISAQARER
                     EDFFTAHYYATVAERLARPDDPNKETAKRAAAEAWNQILQGFAELQSRGDKDLYTTKR
                     QGYEAIQGGDYLRAYYIFIDLKNKDIARQDGKHDPDIDRFLELSQSGVLESFFFFDET
                     SDLGRFEASRDIFFTLSRSGGTDVIYIKGVTYSRQSGRDVAYLRDVSFARFDSFNKLQ
                     YKMYVPQAKMFAFADDGKNPRPQILLRSVGRESDGVVTRPVVLEGSAPAQDQAVLLLD
                     MPLRDFSLIVSANRGPASMIMTDLFRFSGRGKQYGYDSPVYLGEALTRLADPFLLLIL
                     SILSLVAGWRYRLGKDVLFKAWWILVVPLFPLLSLIVIGFIRYLSALVIVAFVSLFPS
                     ISVVLTLAFLAVCFAAASMYFSAQRSD"
     gene            complement(3309..>3715)
                     /locus_tag="PKH81_02070"
     CDS             complement(3309..>3715)
                     /locus_tag="PKH81_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96853.1"
                     /translation="FSRPMVIARLQDSALAVMNGFSALIRDGFITYLVGAAAFYFTVF
                     SLWVICFSTAWRLLNILLLYSGFIFCCVLWPVLEQDRVTMLLLSVMPSWLGRSAYVPL
                     VYASFSLLVLMVALGVFITRLVRRRARGVSYG"
BASE COUNT         1097 a          932 c          891 g          795 t
ORIGIN      
        1 ctggaaaaag ctgctacacg cgcaggtgtt gccgttatta aaaaaccaat cagaggcggc
       61 acagacggtt cgcgtctaac cgaaatgggc ataccaacac cgaatatctt taccggcgga
      121 cataactatc actcgcgaag tgaatgggca agtattgaac aaatgggtaa aaccgtggaa
      181 acccttatac aactggcgat tatctgggga gaggatcatg tacaagtaca aaattgaggg
      241 cgggttcccc atcaagggaa ctatcaaagc aagtggaaat aaaaacgcag cccttccatg
      301 tatagcagcg gctctattaa ccgacaaacc ggttatcctt cgcaacctgc cggagataga
      361 agacgtacag gttatgttgg atgttttttg cgcacttggc ggctcggtag aaacacttca
      421 agatggggcc aagcgtctaa gcatagcaag cttgtcgcgc actgatattc cgcaagatct
      481 ttcccgcaag atccgcgcat ccattctctt tgccggcccc ttactcgctc gagcagggaa
      541 ggcagttttg ccacctcctg gtggagatgt tatcggccgc agacgcttag acacccactt
      601 tcttgcctta acagagcttg gagcccgggt cgagatagac ggacaattca cctttaccgc
      661 caataagtta aaagggcagg atatatttct ggacgaggct tcggttaccg caacagaaaa
      721 cgcggtaatg gcggctgtac tcgcggaagg gcagacaatc ttgaccaacg ccgcaagcga
      781 gcctcacgta caggatctat gcgccatgct taactcaatg ggcgcttcta tctccggaat
      841 cggctcaaac atcttgacta tagaaggggt acaaaacctt tctggcacag acttccgcat
      901 cggcagtgat tacatggaga tcggctcgtt tatcggccta gctgccgtta cccatggatc
      961 gataaccata acagacgtac aaccccgaga ccttaggccg gttaggcttg cctttggtaa
     1021 acttggtatt cgatgggata ttgaaggcac cactctaacg gtaccatctc agcaatctct
     1081 tcagattaat agtgatcttg gcggaatgat tccaaaaatc gacgacgcac cctggccagg
     1141 attcccgccc gatttaacga gcatcatgac cgttgtggcc acacaggtag aaggaacagt
     1201 acttattcat gaaaaaatgt ttgagtcgcg tatgttcttt gtggacaaac ttataggcat
     1261 gggtgcgcgt ataacgctct gcgatccgca tcgcgcggta gtctccggtc caagcgtcct
     1321 tcatggatca gaactggtat cgccggacgt acgggctggt atggcaatgg tcattgccgc
     1381 actgtgtgcg cgaggggaaa gcgttatcag taatgtgtat caaatagagc gcggatacga
     1441 aaatctgact acacgccttg tatcccttgg cgcccacata gagcgtattt cgtcctgtta
     1501 aagtgattat tgggcccgtt ccactcggag cgggtcaatc ggagcgttgc gcggagaaat
     1561 acatagaagc cgcggcaaag catacagcca aaaaggccag ggtgagtaca acagagattg
     1621 acgggaacag ggaaacaaag gccacgataa ccagtgcaga cagataccgt ataaacccga
     1681 taacgattaa cgataacagc ggaaacaggg gtacaacaag tatccaccaa gccttaaata
     1741 acacatcctt tcccagtcga tagcgccatc cagctaccaa agaaagaata ctcaaaataa
     1801 gtaataaaaa cggatctgca agcctggtta aggcctcgcc aagatatacc ggtgaatcat
     1861 agccatattg ctttccccga cccgagaaac ggaataaatc tgtcattatc attgacgccg
     1921 gtcctcggtt ggcactgaca atcaaggaaa aatcgcggag aggcatatca agcaataata
     1981 ccgcctgatc ctgggctggc gcgcttcctt caagcacaac gggacgggta acaacaccat
     2041 ctgattctcg ccctaccgat cgcagtaata tctgcggccg aggatttttt ccgtcatctg
     2101 caaacgcgaa catctttgcc tggggaacat acatcttgta ttgcagcttg ttaaaagaat
     2161 caaagcgtgc aaaggaaaca tccctaagat acgcaacgtc cctacccgac tgccgactat
     2221 aggtgactcc cttaatgtag ataacatcag taccgccaga gcgggaaagg gtaaagaaaa
     2281 tatcgcggga agcttcaaaa cggccaagat ccgaggtttc gtcaaagaaa aagaaggatt
     2341 caaggactcc gctttgggac agctcaagaa accggtctat atcaggatca tgcttgccat
     2401 cttgacgggc aatatccttg ttttttaaat caataaagat gtaatacgcc ctcagataat
     2461 ccccaccctg aatggcttcg tagccctgtc tttttgtcgt atataaatct ttatcgcccc
     2521 gggattgcag ctctgcaaag ccctgtagta tctggttcca ggcttcagcg gcagctctct
     2581 tggccgtttc tttgtttgga tcatctgggc gggcaagccg ttcagccact gtcgcataat
     2641 aatgagcagt aaaaaagtct tcgcgctcgc gagcctgtgc cgatatatcc agggctccaa
     2701 gtacggttaa ccccgtcgca tcggcaataa tgagcgggtc cggaagtcgc gactgtcgag
     2761 agagcccttc cccctgtttc tccgctatgc ggagccggca ggattctcgt aaatccgcta
     2821 cttcctcgct atccttccaa ataccctctg cggcaacaag atatccatca gctcgcccat
     2881 gatcgtcctt taccatcagc gatcgtgcta ccgacacata ttcccgatag tcggttgttc
     2941 gggtaatagc tgtttcgttt ctggttcgca aggaaggagc aagcccttcg gcaaggatga
     3001 catagataca gagtaaaaaa agggaaaggg caaaggcgtc tttaagacaa gaaaacaagc
     3061 cctcggacca acgctcaacc ttaaggtcgg acactttgga aaacgcaagt gcatatccta
     3121 caagaattcc gcttacctgt aatgaaggaa aatactggat aaacaacagg atcgaacttt
     3181 ttatgcgata cggtgtaata aagggaggaa gaaggctaat atcaagggtg gaaacaagag
     3241 aatatacgac gcatatagcc aaaccgataa gcagatgggc aatgactatc ggggtaatcc
     3301 gtttaagttc aaccatagga cactccgcgc gctcggcggc gtacaagacg ggtaatgaac
     3361 actccaagag ccaccatcaa taccaacaag gagaaagagg catacaccag gggtacatac
     3421 gcagaacgtc ccagccaaga tggcataaca gacagtaaaa gcatcgtaac ccgatcttgt
     3481 tcaaggactg gccacagcac acaacaaaaa ataaaaccgc tatagagcag caatatattc
     3541 aaaagacgcc atgcggtgct aaaacaaatg acccacagag aaaacaccgt aaaatagaaa
     3601 gctgcagcgc cgaccagata ggtaataaac ccatcgcgaa tcagggcaga aaatccattc
     3661 ataaccgcca aggcggaatc ctgaagccgg gcaatgacca tcggacgaga aaacc
//
LOCUS       DAOQXQ010000080         3709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25762_length_3709_cov_2.873563, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000080 DAOQXQ010000000
VERSION     DAOQXQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25762_length_3709_cov_2.873563"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKH81_02075"
     CDS             complement(<1..874)
                     /locus_tag="PKH81_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HNQ96854.1"
                     /translation="MEALIKAIEQVNGVVNAMVWGPPMLALIICTGVFLTIKTRFFQI
                     SHAKEVGEHTFFAIFKKKSVRSTKEKKAITQFQALSTALAATIGTGNIAGVATAISLG
                     GPGAVFWMWVSAFFGMMTNYSENVLGIYYRKKNVKGEWAGGAMYYLKYGLGEKKGFGA
                     IAKPLAVIFSVFCVLASFGIGNMTQVNSIASALDGSFKIPHLVTGAVLAVLAGLVIIG
                     GIKRIAKVTERFVPFMAFAYTIGALIILCVNIQAVPHVFGQIFRGAFGFQAAAGGFAG
                     AAMKQAITMGFKRGV"
     gene            1023..2063
                     /locus_tag="PKH81_02080"
     CDS             1023..2063
                     /locus_tag="PKH81_02080"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ96855.1"
                     /translation="MTRFQQQKVNKLLLITAISSSLGGLFAYIMVLLLSTPGSRTHDI
                     QYLLLGSLTGFFISSTLISISWKMDSWARKPLWFDILIKPLMQTMLIILIYSTIYICI
                     FGLQMFQKESWILPTLLFSFIMFALITFFENLERLLGKNVLKGLILGTYRKPVRENRF
                     VMFLDIAGSTSLAERVGDVAFHALLNDFFCEIARPIVNNQGEIYKYVGDEVIITWKES
                     TGKYKDAALAVLHAIDRTLERKKEYFISRYGTMPEYRAGLHFGQVVAGEMGLTRQEIA
                     FSGDVMNTASRIQGECRNLGERFLVSEAAVVGLGIREGKTSNGELCSRGSIALRGKKE
                     ELNVYALHCNAD"
     gene            complement(2045..2758)
                     /locus_tag="PKH81_02085"
     CDS             complement(2045..2758)
                     /locus_tag="PKH81_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ96856.1"
                     /translation="MFRTVIAIVRFFWCLIKKIPKMRQALAFDRKGMIAERDAVVNEQ
                     VTHWARFVLALAGGEVEVVGEEKIPQDTAVVFVGNHQSYFDIPILLGFISKPKAFISK
                     IEILKVPFLSVWMRLMQCTFLDRKNMRQSIEAMNEAAKTVQKGYSLVIFPEGTRSKGK
                     GFGSFKAGSFKLALKTGVPIVPVTIDGSWRLFEEKGHVARATVRLIIHDPVPTANLSR
                     EESQELPALIQSRVQSALQ"
     gene            complement(2759..>3709)
                     /locus_tag="PKH81_02090"
     CDS             complement(2759..>3709)
                     /locus_tag="PKH81_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNQ96857.1"
                     /translation="RKLGLKGMSKEELPRFLPLFLTKGYLMLPLGVILFFLVQGKTAT
                     FSALMGIVSCAILGIVASCVDIARGKSPRMPLSDFVDIMVSSARNIIAVAIACGMAGI
                     IIGIVTLTGIGLKLGAGLIAISGGIPIITLMLTMISSIILGMGAPTTANYLITSTITA
                     GAIVGLGYEPLAAHMFAFYFGIIADITPPVALAAIAGSAIAKSKPMATALQATKIAIA
                     AFIIPYMFVFNPEMLLIGTTWSNVILIIVSALIGMFGLGAALEGYTSTHCRPLTRVLL
                     GGAGLLLILPGLQTDIIGFSIIVVVLAAQVVAQKIARRRA"
BASE COUNT         1080 a          933 c          813 g          883 t
ORIGIN      
        1 aaacaccccg cttaaagccc atggtaatag cctgcttcat cgcagctccg gcaaatccac
       61 ccgcagcggc ttgaaagcca aaggcgcccc ggaagatttg tccaaaaaca tggggaacag
      121 cttgaatgtt aacgcagaga ataataagag cgccaatggt atacgcaaag gccataaacg
      181 gaacgaaccg ttcggtaacc ttggcgatgc gcttaatgcc accgataatc acaagaccgg
      241 ccaataccgc aaggacagcg ccagttacca gatgaggaat cttaaatgag ccatcaaggg
      301 cactggcaat agagtttacc tgagtcatgt tgccaatacc aaaggaggca agaacgcaaa
      361 acacagaaaa gatcaccgca aggggttttg caatggctcc aaagcctttc ttttctccaa
      421 gcccgtattt caaataatac atggctccac ctgcccactc cccctttaca ttctttttcc
      481 gataataaat acccagtacg ttttcggagt aattggtcat catgccaaag aacgcagaaa
      541 cccacatcca aaacaccgct cccggtccac cgagcgaaat ggcagttgcc acaccggcaa
      601 tattgcccgt tccaatggtt gccgcaagag ccgtggacaa ggcctgaaac tgagtaatcg
      661 cctttttctc tttggttgac cgtaccgatt ttttcttgaa aatcgcaaaa aaggtatgtt
      721 cgccgacttc tttggcgtgc gagatctgaa aaaaccgcgt tttaatcgtt aaaaatacgc
      781 cggtacaaat gataagcgcg agcatcggag ggccccacac cattgcgttg acaactccat
      841 tcacttgctc aatagccttg attagggctt ccatacaaaa cctccaaaaa acaaattctt
      901 ctaagaatgc catgaataaa agccattacg caagaaaaat cgtgtcccta cgcgagcaat
      961 taccaacaat cttgtaagca ctccatttgc gtttacggga aagtaaggat atgatacaca
     1021 ccatgacccg atttcaacag caaaaagtga acaagctcct ccttattacc gctatcagta
     1081 gcagccttgg cggtcttttc gcctatatca tggtgcttct tctttccacg cctggctcac
     1141 gcacccatga tatccaatat ctgcttttag gctcccttac cgggttcttt atttcttcta
     1201 ccctcatctc aatttcctgg aagatggata gttgggctcg aaaacccctc tggtttgata
     1261 tcctgatcaa acccctgatg cagacaatgc tcatcatcct tatttacagc accatctaca
     1321 tatgcatttt tggattacag atgtttcaaa aggaatcatg gattctacca acacttctgt
     1381 tttcttttat catgtttgcg cttatcacct tttttgaaaa tcttgaacgc cttttgggca
     1441 aaaacgtact taaaggactt atcctgggga cctaccgcaa accggttcga gaaaacagat
     1501 tcgtcatgtt tttggatatt gccggatcaa cctctcttgc ggagcgagtt ggagatgtag
     1561 cctttcatgc cctgttaaat gatttttttt gcgagattgc ccgtccaatc gtaaacaacc
     1621 agggagaaat ttataaatat gtcggcgatg aagtcattat tacctggaaa gaatccacgg
     1681 gcaaatataa agacgcagcc cttgcggtat tgcatgctat agaccggacc ctggagcgaa
     1741 aaaaagagta ttttatcagt cgctatggga caatgcctga atatcgggcc ggccttcatt
     1801 ttggtcaggt agtcgccgga gaaatgggtc ttacccggca ggagattgcc ttttccggag
     1861 acgtgatgaa caccgcttca agaattcaag gggaatgtcg caatctggga gagcgttttt
     1921 tggtttccga agcagcagtt gtgggcctcg gaattcggga agggaaaaca agcaatggtg
     1981 aactgtgttc ccgcggttcc attgccctac gaggcaaaaa agaagagctt aacgtttacg
     2041 cccttcattg taacgctgat tgaacccttg attgaatgag ggcgggaagc tcttgtgatt
     2101 cctcccgtga aagattagcc gtaggcaccg gatcatgaat gatcagccgg accgtagctc
     2161 tggccacatg gcccttctct tcaaaaagcc gccatgaacc atcgatcgtt accggtacaa
     2221 tgggcacacc cgttttgagc gcaagcttaa agcttcctgc cttaaaagag ccaaagcctt
     2281 ttcccttact tcgcgttccc tccggaaaaa taacaaggga gtatcccttt tgcacagttt
     2341 tagccgcttc attcatcgcc tcgatagact gacgcatatt ctttctgtcc aaaaaggtgc
     2401 attgcattaa acgcatccat acagaaagaa agggaacctt taatatttct atcttggaaa
     2461 taaaggcctt tggcttagag ataaacccga gcaagatggg gatgtcaaaa taactctgat
     2521 ggttccccac aaagacaacg gccgtatcct gaggaatctt ctcttcgcca acaacctcta
     2581 cctctccccc cgctaaagcc agaacaaagc gagcccaatg cgtaacttgc tcatttacca
     2641 cggcatcacg ctcggctatc atacccttgc gatcgaaggc aagggcttgg cgcatcttgg
     2701 ggatcttctt gatcaaacac caaaaaaacc gaacaatcgc gattacggtc cgaaacatct
     2761 atgccctccg tctcgctatc ttttgtgcaa ccacctgcgc tgccaatacc acaacgataa
     2821 tcgagaaacc gataatatcg gtttgcaatc cgggaaggat caaaaggagt cctgctcccc
     2881 cgagtaatac ccgagtgagc gggcgacaat gggtagaagt atatccttcc aatgcagcgc
     2941 caagaccaaa cataccaata agagcagaga caataatcaa gataacattg gaccaggtag
     3001 tgccaattaa caacatctcg ggattaaaaa cgaacatgta cggaataata aaggcggcta
     3061 tcgctatctt tgttgcttgc agcgccgtag ccattggctt tgactttgcg attgccgaac
     3121 cggcgatagc ggcaagggca actggaggag tgatatcggc gatgatacca aaataaaacg
     3181 caaacatatg tgccgcaagg ggttcatagc caagaccaac aatcgctcca gcggtaatcg
     3241 tggaggtaat caagtaatta gcggtagttg gcgcgcccat accaagtatg atggaagaaa
     3301 tcatcgtaag catcaaggtg ataatgggaa tgcctcccga aatcgcgata agaccagcac
     3361 caagcttaag gcctattccg gtaagggtta caataccgat tatgataccg gccataccac
     3421 aggcaatggc aacagcgatg atattgcggg ctgagctgac catgatgtcc acaaagtctg
     3481 acagaggcat tcgcgggctt tttcctctcg caatatccac acaggaagca acgattccca
     3541 ggatggcgca ggaaacaata cccataaggg cggagaaggt tgcggtcttt ccctgaacca
     3601 aaaagaaaag aataacgcca aggggcaaca tcagataccc cttagtaaga aatagcggaa
     3661 ggaatcgggg taactcttct ttactcatgc ccttaagacc gagctttcg
//
LOCUS       DAOQXQ010000081         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25773_length_3708_cov_2.415001, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000081 DAOQXQ010000000
VERSION     DAOQXQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25773_length_3708_cov_2.415001"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKH81_02095"
     CDS             <1..1153
                     /locus_tag="PKH81_02095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ96858.1"
                     /translation="ASSYVDIKKNRSLAGPLFDRYTTLFERLSEECRNKPKGITPAYI
                     NPDGSSGYSFLLMKLRMLLEHKKRYRELYGSLPELILPAFQMTSVHTDAAIATAFKEY
                     VLCDELRGLAHEIGCPSIEIFTESQTMMAATTHSSQGVPRKIFDCAHGQANTPIPLPG
                     GHGQNFSILAPIYRKLRESGVRWVWLGNIDNMGYTVCPESLAIFALSGSGAAFETSVR
                     TAMDVKGGILVSDAKGRLTCADIGPAISADTVVSLEEAGKTVLFNCGVGLFDLEWLCP
                     HLDEIPYKLPLRVTDQDKDSGLYAQAEQITWEIIGLVENPLFFAVRKDKRFIAAKLLM
                     ETIMTSVPPTQESLSPEIAQVSHLLHDGLLSLLEGEYGLVRQGGRLVPR"
     gene            1264..2502
                     /locus_tag="PKH81_02100"
     CDS             1264..2502
                     /locus_tag="PKH81_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ96859.1"
                     /translation="MKKSVCALVAVLLCLSQVFAQQVDLEMYYYKQENQEGLKKLVET
                     YTAKTGVKISLLIVPNDADATMSARAAQGKLPDILQMQTYARVWEYAQKGYVMDISKQ
                     PVLDKVLDSAKPSVTWNGKQYALPMDYAGIGIIYNKEIFAKYKLKEPKTARELERVCA
                     TLRKNGVTPFAGLLNENWSAGHFITLVHTALLVGDKKIPVDRFVADMNTGSSSFGVVD
                     SSKLFAALDFYRDNMDPKAAEWGWNEQQAAFAQGKAAMMVQGLWSYGAAIATNPKLDC
                     GFIPYPVFNKASDNKMYADIDSCFGVSAQSSKEKQQAALAFLEWLATPEAQKIWIEDY
                     KLVPAFKGANLSSMKKPFQDLLASVDKNGAYPWAFASYPTVVFEDACKNGAQQYMFKK
                     TTADAVVKTVDETWAKAVKK"
     gene            2575..>3708
                     /locus_tag="PKH81_02105"
     CDS             2575..>3708
                     /locus_tag="PKH81_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96860.1"
                     /translation="MVDSHFRAKANFALFIIPAFVLYSLFFIVPFFRGFAISLTNWDG
                     LTPRAPNVLSSTDFETKVLARLERPADREFLLSVYTLSDDGQTWTRRALSGFLNRRRA
                     ERVFSRAGFQSESNRYIGLKNYKDILSGNIGSSFYPRMYTQYYYNSTSALPALLDDPS
                     FGTHFLSRLTSDERKVFDRYYQLSDGGYELIETDVEFYLEDLIWYLPEITDTETLSET
                     DASDFIDAIRRSGVAQNHGEYQSVVEDFLGSHELSTQSRDAVHQAALGLYEVGDIKAL
                     LSSRWNSRKIDLGVIGFTLFFAFFSVIGINILAFVLALALDSGLRGQKALRSIFFLPN
                     VLSMIIVALVWKMLFFQLFPRLTGVDQWLSDPAKTPSLIVLVAI"
BASE COUNT          905 a          869 c          909 g         1025 t
ORIGIN      
        1 ggcctcgagt tatgtggaca ttaaaaagaa tcgctccctt gccgggccgc tgtttgatcg
       61 ctataccact ttatttgaac gtttatccga agaatgtcgt aataagccca agggcataac
      121 tccggcgtac ataaatcctg acggtagttc cgggtactcc tttttattga tgaagctccg
      181 tatgctgctt gagcacaaga agcgatatcg tgagctatac ggatcccttc ccgagctcat
      241 tcttccggcg tttcaaatga caagcgtaca cactgacgcg gcgattgcga ctgcttttaa
      301 ggaatacgtc ctttgtgacg agctcagggg tttagcccac gagattggct gtccgtccat
      361 cgagattttt accgaaagtc aaacgatgat ggcagccact acccattctt cgcagggcgt
      421 acccagaaaa atttttgact gtgcccatgg acaggccaac acaccaatcc cccttccggg
      481 aggacatggt cagaattttt caatcctggc gcccatttac cgtaagctcc gcgaaagcgg
      541 ggttcgttgg gtctggctcg gaaacatcga caacatgggc tatacggtat gtccggaaag
      601 ccttgcgatc tttgctcttt ccggttccgg ggcggctttt gaaacctctg ttcgcacggc
      661 tatggacgtt aagggcggta ttcttgtttc cgatgcaaaa ggtcgcctta cctgcgctga
      721 tatcggtccc gctatttccg ccgatacggt cgtatccctg gaagaggccg gaaaaaccgt
      781 tctgtttaat tgtggggttg gcctttttga tcttgaatgg ctgtgtcccc atcttgatga
      841 aatcccctat aagcttcccc tgcgggtaac cgatcaggat aaggatagcg gtttatacgc
      901 ccaggcagag caaataacct gggagattat tggattggtc gaaaacccgc ttttttttgc
      961 ggttcgcaag gataagcggt ttattgccgc aaagcttctt atggaaacaa tcatgacgag
     1021 cgtccctccc acacaagagt cgctctcccc cgaaatagct caggtgtctc atcttcttca
     1081 cgatggctta ctgtccctgc ttgaaggtga atacggcctg gttcgtcagg gcggcaggct
     1141 cgttcctcgt taaatagcaa taattctgtt gacaaatagg taaaccggtt taataatgag
     1201 gtaaagcggt ttaataaacc gttttaatag tgttccaatg agagagaaga gaggaggaga
     1261 gcaatgaaaa agagtgtatg tgcgctggtg gccgtgttat tgtgcttgag ccaggtattc
     1321 gctcagcagg ttgacctcga gatgtactac tacaaacagg aaaatcagga agggctcaag
     1381 aagctggtgg agacctatac cgcaaaaacc ggggtcaaga tttctctgtt gattgtcccg
     1441 aatgacgctg atgcgaccat gtctgcccga gcggcccagg gaaaattacc ggatatcttg
     1501 cagatgcaaa cctatgcccg tgtatgggaa tacgcgcaaa agggctatgt aatggacatc
     1561 tccaagcaac cggtattgga caaggtgctc gatagtgcta agccttcggt aacctggaac
     1621 ggtaagcaat acgcactccc gatggactat gccggaatcg ggattatcta caacaaagaa
     1681 atctttgcca aatacaagct aaaggagcct aaaacagcgc gagaacttga gcgagtgtgc
     1741 gctaccttac ggaaaaacgg ggtaactccc tttgccggac ttttaaacga aaactggtcg
     1801 gcaggacatt ttattaccct ggttcatacc gctttactcg ttggagacaa gaagataccg
     1861 gttgatcgct ttgttgcaga tatgaatacc ggttcatcaa gttttggcgt tgtcgattca
     1921 tccaagcttt ttgcagccct ggacttttat cgggacaaca tggatcccaa ggcagcggaa
     1981 tggggatgga acgagcaaca agctgcgttt gctcagggaa aggcagccat gatggttcag
     2041 ggtctgtggt cttatggtgc ggccattgcg acaaacccca aactcgattg cggttttatt
     2101 ccctatccag tatttaacaa ggcttccgac aacaagatgt atgccgatat cgactcctgt
     2161 tttggggttt cggctcaatc atctaaagag aagcaacagg cagcgcttgc cttccttgaa
     2221 tggcttgcga cccctgaggc tcaaaagatt tggatagaag attacaagct tgttcctgcc
     2281 tttaagggtg cgaacctttc cagcatgaaa aagcccttcc aggacttact cgcgtcggtt
     2341 gataaaaacg gcgcatatcc ttgggccttt gcttcatatc caactgtcgt atttgaagat
     2401 gcctgcaaaa atggggctca gcagtatatg ttcaagaaga ccaccgccga cgcggtagtc
     2461 aagactgttg acgaaacctg ggctaaggcc gtcaagaaat aaacgcacgg tgaatacagc
     2521 gcgggcactt cgggatattc cgaatcccgc gctttttttt aaaatggagt aagtatggta
     2581 gacagccact ttcgcgcaaa agcgaacttt gcgctattta ttatcccggc ttttgtgttg
     2641 tactcgcttt tttttatcgt tccttttttt cgtggctttg cgatatccct gactaattgg
     2701 gacggcttaa caccgcgcgc gccaaatgta ctatccagca cggactttga aaccaaggtc
     2761 cttgcgcgcc ttgagcggcc agccgacaga gaatttcttt tatcggtgta tacgttaagt
     2821 gatgatggtc agacatggac tcgacgcgcg ctatcgggtt ttttgaatcg aagaagagcc
     2881 gaacgcgttt tctctcgcgc aggctttcaa agcgagtcaa accgctatat aggccttaaa
     2941 aattacaagg acattctttc gggaaatatt ggttcttcct tttatccccg tatgtatacg
     3001 caatattact ataatagtac ctcggcattg cccgctcttc ttgatgatcc ttcgttcggt
     3061 acacattttt tgtcacgcct tacctccgat gagcgcaagg tatttgatcg ttactaccag
     3121 ctgtccgacg ggggctatga acttattgaa actgatgtag agttttatct tgaggacctt
     3181 atttggtatc ttccagaaat aactgataca gaaacacttt cggaaacaga cgcgagtgat
     3241 tttatcgacg cgattcgtcg ctccggtgtt gcacaaaacc acggagaata ccaatccgtt
     3301 gtagaagatt ttttgggtag tcacgagctc tctacacaaa gccgggatgc agttcaccag
     3361 gcagcccttg gtttatatga ggtaggggac atcaaggcct tgctatcttc ccgctggaat
     3421 agtcggaaga ttgatcttgg agtcattggt tttaccctgt tcttcgcctt tttctcggta
     3481 attggcataa acattcttgc ctttgttctt gcccttgccc ttgatagcgg gctgcgtggt
     3541 caaaaggcgc tacggtctat ttttttcctt ccgaatgttc tttcaatgat tattgttgcc
     3601 cttgtgtgga agatgctttt ctttcaattg tttcctcgcc ttactggagt ggatcaatgg
     3661 ttaagtgatc cggctaaaac cccgtctctt attgtgcttg tagccatt
//
LOCUS       DAOQXQ010000082         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_25791_length_3706_cov_2.637086, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000082 DAOQXQ010000000
VERSION     DAOQXQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25791_length_3706_cov_2.637086"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKH81_02110"
     CDS             complement(<1..1070)
                     /locus_tag="PKH81_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HNQ96861.1"
                     /translation="MAEVADTMVPRWNLESIFVGFDDPVYLSALEELSSGAKKFERLL
                     AEVPQEGFAHKGGDIAAAEWLTRMIELRSRIHILSETLSSFCYARYSVDTTNTQAMNN
                     LNRVEELLVPFNRLLTLYLNALKDNADVITALFTRGGDLQQYQHLIEDEIFWQARQMS
                     PAEEDLAADLARSGASAWSRLQEQMTSTASALWDEKTGERKTLVELRALAHDANREIR
                     KRAWEKEIELCKTISLPVASALNGIKGATITLNGRRSWKGSSFEAAIAKSTRQGLLSE
                     RSLSALIHAMEEALPHWRRYLGAKARLMGLSSCAFYDLFAPVGNAGTRWTFDKARKRI
                     CEIFADFSPRMGAFAKRAFAES"
     gene            complement(1063..2718)
                     /locus_tag="PKH81_02115"
     CDS             complement(1063..2718)
                     /locus_tag="PKH81_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNQ96862.1"
                     /translation="MNELVLLGDEALALGALHSGVSGCFGYPGTPSTEILEYLVDNFE
                     RASGPVAQWCANEKTAYESALGVSFAGKRAIVTMKHVGLNVAADPFMNSSLCQIKGGL
                     ICVVADDPSMHSSQGEQDTRYYADFAMVPLFEPINQQEAYEMVAEAFEISERLHVPVI
                     MRMVTRLSHSRAPVHPVANIRSQNDLEKGSPADWMLLPALARKNYLKMISKQEELVSW
                     SCGYARTHLTLNPKRTDIAIITSGLGHNYFEENRSDYTALCADKGLPSVLRIAALPLP
                     LEHIRAIAQTAQTILVIEEGMPFIERQLRGLVPSHNQIIGKFTGHLPRTGELNPDLVR
                     TALGLPAHHSVSASFNPADLPGRPPQLCKGCPHHDSYSALNAAVELLNKKEGKVSTVV
                     TADIGCYSLGATNPLKAIETIVCMGASIGMARGASLAGYKYAFGVIGDSTFYHSGLTG
                     IVDAVSTKTPVTIIILDNSIVAMTGCQPTMLPSSQLEKVLLGLGIEREHLRVIETKPG
                     TVEENTRILVEEAEYRGPSVIIMVRECLEAFRLRKKKDAITNG"
     gene            complement(2818..>3706)
                     /locus_tag="PKH81_02120"
     CDS             complement(2818..>3706)
                     /locus_tag="PKH81_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96863.1"
                     /translation="LSTVTSAAERLSALRARMESVLDQLSMEVLSAPSETVSGKAFKT
                     DFIARLVHISDSGAITVQNTPCVVVYPFVGEDGTPGTKMEHVVTDANGQVRFTAPVPS
                     QTGKNKLYIACALTSDDPVIKESLTARRESGRLSVSFTHAVGTAQRSVPTTISILDFN
                     QTGAPIKSSNPSATTLLKPLVQRGFTRIGMADFPNQLASGDEAALIRAATAQFGTAVQ
                     RFIYGTVRIESLAQGEDQLWSCALVAQISVWDFSKGQKVLSLSLRQSEAGPTAARAID
                     SARKKLAGEVLVDSLVFGL"
BASE COUNT          938 a          950 c          975 g          843 t
ORIGIN      
        1 caggattccg caaaggccct tttggcaaag gcacccatgc gtggtgaaaa gtcggcaaag
       61 atttcgcaaa ttctttttcg cgccttatcg aacgtccatc gcgtgccggc gttcccgacc
      121 ggggcaaaca aatcgtaaaa ggcacaggaa gaaaggccca tgagccgagc cttggcacca
      181 agatagcgtc tccaatgggg cagggcttct tccattgcgt gaataagagc agaaagagat
      241 cgctcgctca ataatccctg acgggtggat ttggcaattg ccgcttcaaa cgaagagccc
      301 ttccaggatc ttcgtccgtt cagcgtaatc gtcgctccct ttataccgtt gagcgcactg
      361 gcgaccggca aggaaatcgt cttgcaaagc tcaatttctt tttcccatgc ccttttccga
      421 atctcgcgat tggcgtcgtg agcaagggct cgtaactcca ccagggtttt tcgctctccc
      481 gtcttttcat cccatagagc cgaggcggta gaggtcatct gctcctgaag tctgctccag
      541 gcagaagctc cgcttcgcgc cagatcggcc gcaagatctt cctcggctgg actcatttgc
      601 ctggcctgcc aaaaaatctc gtcctctata agatgttgat actgctgtaa atcgccgcca
      661 cgagtaaaaa gagcggttat aacatcggca ttgtctttta gcgcattcag gtacagcgtg
      721 agcagtcgat taaagggaac cagtaattcc tctacccgat taaggttgtt catcgcctgc
      781 gtattggtgg tatcgactga gtaacgtgca taacaaaacg agctaagcgt ttcgctcagg
      841 atatgaatcc tgctgcgcaa ctctatcatc cgggtgagcc attcggcagc ggcgatgtct
      901 ccgcccttgt gggcaaagcc ttcttgagga acttcggcta agagacgctc gaacttttta
      961 gcgccagaag aaagctcctc caacgccgaa agatatacag gatcgtcaaa accgacaaag
     1021 atactctcaa gattccatcg gggaaccatg gtgtctgcga cttcagccat tggtgatggc
     1081 gtcctttttt ttacgcagac ggaaagcctc aagacattcg cgaaccataa ttataaccga
     1141 aggtccgcga tactccgctt cttccaccag gatacgcgtg ttttcctcaa cggttccagg
     1201 ctttgtctcg ataacccgca agtgctcgcg ttcaatgccc agtcctagca gaaccttttc
     1261 aagttgactg gagggaagca tggtgggctg acagccggtc atggcgacga tggaattatc
     1321 aagaatgatt atggtaaccg gcgttttagt tgacactgcg tccacaatgc ccgtaagacc
     1381 tgaatgataa aaagttgaat ccccgataac gccaaaggca tacttatatc cggcaagaga
     1441 tgcgccacgg gccataccaa tggaggcgcc catacagacg atggtttcga tggccttaag
     1501 gggattagtc gcaccaagtg agtaacagcc aatgtcggca gtaaccacag tggacacctt
     1561 tccctctttt ttattaagaa gctcaacggc ggcatttaat gcggaatagg aatcgtgatg
     1621 tgggcagccc ttgcagagct gaggcggacg acccggcaag tctgcgggat tgaacgaagc
     1681 gctcacgctg tgatgagcgg gaaggccaag ggcagtgcgt accaagtcgg gattaagctc
     1741 tccggttcgg ggaagatggc cggtaaactt tccgattatt tgattatgcg agggaacaag
     1801 gccacgcaat tggcgttcaa taaaaggcat gccttcttct ataaccaaaa ttgtttgggc
     1861 ggtttgcgca atggcacgaa tgtgttccag gggcagagga agggctgcta ttctgagcac
     1921 agagggtaga cccttgtctg cgcacagagc cgtataatcg ctgcggtttt cctcaaagta
     1981 gttgtgtcca agtccactgg taataatcgc aatatccgtt cgcttcggat taagggtcaa
     2041 gtgtgttcgc gcatagccgc acgaccagga taccagctct tcttgtttgg aaatcatctt
     2101 gaggtagttc ttgcgggcca aagcgggaag aagcatccag tccgcaggag agcccttctc
     2161 aagatcgttt tggctcctga tgttggcaac aggatgaaca ggggcgcgtg agtgcgaaag
     2221 tcgggttacc atgcgcataa taacgggaac atgtaatcgc tcggaaattt caaaggcctc
     2281 tgccaccatc tcgtacgctt cttgctggtt aatcggctcg aacaggggaa ccatggcaaa
     2341 atccgcatag taacgggtat cctgttcacc ctggctagaa tgcatcgaag ggtcatcggc
     2401 gaccacacag ataagaccgc ccttgatttg acaaagggat gagttcataa agggatcggc
     2461 ggcgacatta agcccaacgt gcttcatggt aacaatagcg cgcttgccgg caaaggacac
     2521 gccaagagca gactcgtacg cggttttttc gttcgcgcac cactgagcaa ccggaccgga
     2581 agcacgctca aaattatcaa ccagatactc aagaatctcg gttgacgggg taccagggta
     2641 cccgaaacaa ccggatacgc ctgaatgaag cgctccaagg gcaagcgctt cgtcacctaa
     2701 aagaacaagt tcgttcatgt gaaaaactcc aggtatgaat gtagccgata ggctctcgat
     2761 gcgtctcagc aggggtatgg taccagcaag tcccgcttgt tgcaagcggg aaacaactta
     2821 gagcccaaag acgagcgagt cgacgagtac ctctccagcg agcttttttc gtgcgctgtc
     2881 gatcgcacgg gcagcagtag ggcctgcctc gctttgccgt agagacaaag aaagaacctt
     2941 ttgtcccttg gaaaagtccc atacagatat ctgggccacc agagcgcatg accacagctg
     3001 atcctcgccc tgagcaagag attcaatgcg aaccgtccca taaataaacc gttgcacggc
     3061 tgtgccaaat tgtgccgttg cggcgcgtat caacgccgct tcgtccccgg aagcaagctg
     3121 attggggaag tcggccatac cgatgcgggt aaacccgcgt tgtaccaggg gcttaagcaa
     3181 tgtcgtcgca gaaggattac ttgatttgat aggggcgcct gtctgattaa aatcaagaat
     3241 tgatattgtg gtgggaacgc ttcgttgcgc agttcctact gcatgagtaa aggaaacaga
     3301 taggcgacca gactcccggc gtgcggtcag ggattctttg ataaccggat catcgctggt
     3361 gagtgcgcat gcaatataga gcttgttttt gccggtctga gaaggaacag gtgccgtaaa
     3421 tcgtacctgc ccgtttgcgt ccgtaacaac atgttccatt tttgtaccag gtgttccatc
     3481 ctctccaacg aatggataca ccactacaca gggagtattc tggactgtaa ttgcgccact
     3541 gtcgctgata tgcaccaaac gtgcgataaa atctgtctta aacgccttac cagacacggt
     3601 ttcgctcgga gccgacagca cttccatgga aagctgatca agaacgctct ccatacgggc
     3661 cctaagggca gaaagccgct ctgccgcaga agtaaccgtg gaaaga
//
LOCUS       DAOQXQ010000083         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26057_length_3679_cov_2.803256, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000083 DAOQXQ010000000
VERSION     DAOQXQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26057_length_3679_cov_2.803256"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1053)
                     /gene="eda"
                     /locus_tag="PKH81_02125"
     CDS             complement(94..1053)
                     /gene="eda"
                     /locus_tag="PKH81_02125"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNQ96864.1"
                     /translation="MESILSEIETVGIVPVIKIDDPLKAVPLARALFQGGMTCVEITF
                     RTTAAAEAIKRISQAVPEIAVGAGTVLSVENAQSALEAGARFAVAPGCNPRVVSWCQE
                     HGLPFIPGVNTPTAIELALEKGITHLKFFPAEASGGVAMLQALSGPFPGVRFMPTGGI
                     SLENIPSYAKCSNVFALGGSWMVNQDLIDKEDWDEIRRRSLQAVTAMQGFSLAHLGIN
                     CKDTDEAKETSAFFSLLGFVPKDGEKSIFNGTAIEVMKSPFLGSMGHIALSCWNIERS
                     LAWLKRHGFSALMETARYDRGQLSVVYLDREIGGFACHLVRSR"
     gene            1179..2204
                     /locus_tag="PKH81_02130"
     CDS             1179..2204
                     /locus_tag="PKH81_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNQ96865.1"
                     /translation="MNTIVTLGEIMLRLKSPSYERLFQSPLLEATFGGGEANVAVSLA
                     CLGLRSSYVTALPEGPIGDAALQQLSRYGVDTSRVIRASGRMGVYFLEAGSCQRPSSV
                     IYDREGSAISLISSQAFDWTRVFSDARWFHCTGITPAISAEAALLSHTAIVAAKKAGC
                     TVSLDLNYRKKLWGYGKTAPSVMGPLVSLCDVLIANEEDIQKSLGIKEPVPASMDDLD
                     GYRALMETVQNHYPNLSYIAITLRQSVSADVNGWMALLRSPQGVYQSRRYDITDIVDR
                     VGAGDAFSAGLIASLAEKPVDAPYALEFATAASCLKHTISGDFNLSTRKEIEALLAGD
                     SSGRVQR"
     gene            2208..3275
                     /locus_tag="PKH81_02135"
     CDS             2208..3275
                     /locus_tag="PKH81_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96866.1"
                     /translation="MKYDIFLESFKQFIQDCPEETLDSSIPYVFFSDLHMGNGSAGDD
                     LEKNRKLLETALEQWYLPRGYCLVLNGDIEDTNKFPLASIQKAWPKLLSLFDSFHQLG
                     RLRKVVGNHDYELLMEKGYPWPLLEGLVFRIGENRLFVFHGHQGSTLYVKFNSISGFL
                     VRYFLRPLHIRNIRVAHDSRRRFRTEKRIYRAARHLGVPVITGHTHRPLFESLSKYDT
                     IRMALEGLLSRYCDADESQKEGIEENIKRYRDELRRLASEKEKTRKTQSLYGGDPFLI
                     PCIFNSGCATGKHGSTALEIYQGEISLVYWTAREESRLYARNEELLGDRLDGGVYRHV
                     LQKDRLDRLFTRIRFLGETGQ"
     gene            complement(3289..3492)
                     /locus_tag="PKH81_02140"
     CDS             complement(3289..3492)
                     /locus_tag="PKH81_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96867.1"
                     /translation="MHYTRTMDAALTLSSLSQLELRHTVCVRTQRMALDATKASGELV
                     LKMMDDVDTITDEALGKQINVQV"
BASE COUNT          921 a          890 c          897 g          971 t
ORIGIN      
        1 gctgtgcgtt tcgtttaaaa actggtcaat acccatattt agtcagtata cctgaattgg
       61 aagcgtcatg gctagtaaaa aagctataca agcttatcgt gagcgaacca gatggcaggc
      121 gaatccgcca atctcgcgat caagatacac caccgaaagt tggcccctgt cgtatcgggc
      181 tgtttccata agagccgaaa acccatgcct tttaagccag gcaagggaac gctcgatatt
      241 ccagcaggaa agggcgatat gacccatgga tcccaaaaag ggagatttca tcacttcaat
      301 cgcggtaccg ttaaaaatag atttctctcc atcctttggc acaaagccta gcagcgagaa
      361 aaaagcagag gtctccttgg cctcatcggt atctttacaa ttgattccca gatgggccaa
      421 cgaaaaaccc tgcatggcgg ttaccgcctg aagggagcga cgcctgattt catcccaatc
      481 ttccttgtca ataaggtcct gatttaccat ccagctgcca cccagggcaa aaacattgga
      541 acacttggcg tacgatggga tgttctccaa gctgatgccg ccggttggca taaatcgaac
      601 cccagggaag gggccggaca gggcttggag cattgccact ccaccagagg cttcggcggg
      661 aaaaaactta agatgggtta tgcccttttc cagggccaat tcaatggctg tgggggtatt
      721 tacgccggga ataaaaggaa gtccatgttc ctggcaccag ctcactaccc ggggattaca
      781 gccgggggcg actgcaaagc gtgccccggc ttcaagggca ctttgagcat tctctacact
      841 aaggacagtc cccgcaccaa ctgcaatttc cggaacagct tggctgattc gtttaatagc
      901 ctcggcggct gcagttgttc tgaaggtaat ttcgacgcag gtcatgcctc cctgaaaaag
      961 agcgcgggcc aggggtacgg cctttagcgg atcatctatt ttaattaccg gaacaattcc
     1021 aacagtttca atttcagaaa ggatgctttc catgacggct ccttggtcaa cttttgaaca
     1081 gtatatcgta tgatctctcg tttgacagca taattctgga tgcgtttatt cgctcctttt
     1141 tcttgactgc accagtacca tcggatagac ttgcccctat gaacaccatt gttaccctgg
     1201 gtgaaattat gctcaggctt aagtcacctt cttatgaacg gttgtttcag tctcccctac
     1261 tggaagctac ctttggtggc ggcgaagcaa atgtcgcggt ttctcttgcc tgtcttggct
     1321 tgcgaagctc gtatgtaacc gcccttccgg aagggcctat cggggacgcc gccctgcagc
     1381 agctgtcgcg ctatggtgtt gatacctctc gtgttatcag ggcttccggt cgtatgggtg
     1441 tgtatttttt ggaagcgggt tcatgccagc gaccatcatc ggttatctat gatcgtgaag
     1501 gtagcgctat ctcccttatc tcgagccaag cctttgactg gacgcgggtg ttctcggacg
     1561 ctcgctggtt tcactgtacc ggcattaccc cggcgatctc tgctgaggcc gccttgttaa
     1621 gtcatactgc gatagtagcg gcaaaaaagg ctggatgtac ggtatcccta gatcttaatt
     1681 accgcaaaaa actgtgggga tacggaaaaa ctgccccttc tgttatgggg cctctggtgt
     1741 ctctctgtga cgtcctgatt gcgaacgagg aagacataca aaaatctttg gggattaagg
     1801 agcctgtacc ggcttcaatg gatgatcttg acggataccg ggcattgatg gaaaccgttc
     1861 aaaaccacta tccgaatctt tcgtatatcg caattaccct gcgtcagtcg gtgtctgccg
     1921 atgtgaatgg ctggatggcc cttttacgct ctcctcaggg ggtctatcag tcccgtcgat
     1981 acgatattac cgatatagtc gatcgcgtag gggcagggga tgccttttcc gccggcttga
     2041 tcgcttccct ggccgaaaaa cctgtagacg cgccctatgc ccttgagttt gcgactgcag
     2101 cttcctgcct taaacatacc atcagtggcg attttaacct gtctacccga aaagaaatcg
     2161 aagcattact cgcaggagat agctccggaa gggtacagcg ttagccaatg aaatacgata
     2221 tcttccttga atcctttaag cagtttattc aagattgccc cgaggaaacc ctggacagct
     2281 cgattcccta tgtttttttt agcgacttac acatgggcaa tggcagtgcc ggggacgatc
     2341 ttgaaaaaaa caggaaattg ctcgaaacag cccttgaaca gtggtattta ccccgtggtt
     2401 actgcttggt tcttaatggc gatatagaag ataccaataa atttcctctc gcttccatac
     2461 aaaaggcatg gccgaaactg ctttctctgt ttgattcctt tcatcaactt ggaaggctac
     2521 gcaaggttgt gggaaatcat gattatgagt tattgatgga gaagggctat ccatggccac
     2581 ttctggaggg tcttgttttt cgtattgggg aaaacaggct ctttgtcttt cacggccatc
     2641 agggttctac cctctatgtc aaatttaatt ctatttcggg ctttttggtt cgctacttct
     2701 tgcgcccttt gcatatccgc aatattcggg ttgcccatga ctcgcgccgt cgctttcgta
     2761 ccgagaaacg catttatcgc gctgcacggc atttaggtgt cccggtaata accggacaca
     2821 cccatcggcc cttgtttgaa tccttatcaa aatacgacac catcagaatg gctcttgaag
     2881 gcttactgtc ccggtattgc gacgccgatg aatcccaaaa ggaaggaatc gaggagaata
     2941 tcaagaggta tcgggatgaa ttgcgccgcc ttgcctctga aaaagaaaag acacggaaaa
     3001 cacagagctt atatgggggg gaccccttcc ttatcccctg tatttttaat tcgggttgtg
     3061 caacgggaaa gcatggatca accgctctgg agatatatca gggagagata tctttggtgt
     3121 attggacggc gagagaagaa agcagactct atgcaagaaa tgaagaactt ttgggagatc
     3181 ggctcgatgg cggggtgtat cgacacgtcc tgcaaaaaga tcgactagac cgcttattta
     3241 cccgaataag atttttaggt gaaacaggac agtgatgttg aaagtgactc acacctgcac
     3301 attgatctgc ttgccaagtg cctcatcagt gatagtatct acatcatcca tcatcttgag
     3361 cacaagctct ccactagcct ttgtggcatc aagagccatt ctttgagttc gcacacatac
     3421 cgtatgacgt aactcaagtt gagataagga tgaaagagtt aaggccgcat ccatagtcct
     3481 agtataatgc accttggctt ttttgtgtat attaaagaca atgcgtacat aaaggagtct
     3541 tacgtgaaca agcgactgat acaggggatt actttctttc tttttttgag catatgtacg
     3601 ggagctgtct ttgcggagaa aaaaatgaaa gctctacccg ataccgttac ctcgggcctt
     3661 accgcgagta aaacaatag
//
LOCUS       DAOQXQ010000084         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26090_length_3677_cov_2.677802, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000084 DAOQXQ010000000
VERSION     DAOQXQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26090_length_3677_cov_2.677802"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..850
                     /locus_tag="PKH81_02145"
     CDS             95..850
                     /locus_tag="PKH81_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96868.1"
                     /translation="MGKTFVFVNQKGGVGKTTSAINIGAYLALEGKRTLLVDFDSQGN
                     MTSGVGVDRDKPTVYDLLAGTVTPKDVIRDSVIPGLSVIPASIDLSGAAIELVDQEKR
                     EYFLKTALEVVKDEYDFILIDCPPSLGILTLNGLVAANEVLIPLQCEYFALEGLTLLL
                     QTVKRVQKTLNPELTIGGIFFTMYDSRTRLAQDVVQQVTSYFKDRVFTTIIPRNVRLS
                     EAPSHGLPICKYDALCVGARSYEKLAKEVLERG"
     gene            843..1748
                     /locus_tag="PKH81_02150"
     CDS             843..1748
                     /locus_tag="PKH81_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNQ96869.1"
                     /translation="MAKQTGLGRGLEALLEESEDKEQTTVATAKGGAGTELFISPSLL
                     KPNPHQPRQEFDEEALRELADSIREHGIIQPIIVEETGNGGYYIIAGERRTRAARLAG
                     LTEVPVIVKKFSEERKLEVALIENIQRENLNPVEEARAYYQLMTLGNLSQEETATRVG
                     KNRSTVANALRLLKLPEDILSSLASGQITPGHARAILAVLNPADQRILFGRIIGNALS
                     VRETERMAAELNGGSRATATKKEKKPADSSRSMEVATLEQKFIDAFGTKVTVKGSLDR
                     GSIEISYFSRDDLDRIYELINACGH"
     gene            complement(1729..>3677)
                     /gene="ppk1"
                     /locus_tag="PKH81_02155"
     CDS             complement(1729..>3677)
                     /gene="ppk1"
                     /locus_tag="PKH81_02155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNQ96870.1"
                     /translation="QVRLISRRVHELVPLQYRCLLQDILPGLEKEGISYVRPEEYTQA
                     QSRFLETFFMNEAFPLLTPLRTSSLGKLPSIGNLRLHAVFHLRPSMDIPEGALADFLE
                     GAGVSVSGDSVDSSGAAARQLFAVVQIPSSIPRVVWLPDASGKRFFTLLDDVVLAFAQ
                     RLFPGFTIEGRLLFKVTRDADSPVDEERDGDFIAAIEEVLANRLSSRPVRLLCTGESP
                     VISAWLLDRMGLSDQDIYRVSGPLDLSTLIDLVETPGLDHLRNPSWNRYWPARFPEDS
                     PLWDTLKATDLLIHVPYQSWEPVLRFLEDASSDPGVLAIKMTLYRTSGDSPVIRSLER
                     AARAGKHVTVFVELKARFDEERNITWVKRLEEAGVIVVYGIARLKVHAKILMVIRREQ
                     EGVRRYVHLSTGNYNDRTARFYSDLSVFTSNPAIANDATLFFNMISGYSAIMGMKYLK
                     MAPIDLKSTLVSLIDREAQRSQAEVPGMIMAKMNSLADPEVVDALYRASRAGVKILLN
                     IRGICTLVPGVKGMSEGITVVSVIDRFLEHSRIFWFQNGGADELFLSSADWMPRNLDR
                     RVELMFPILQESIRKEIRSLLGMYFEDNLKAHRLTSEGTWTRLSPSGSETPMRVQERL
                     YLEEKRRSELQEKEPRRDFNVRRH"
BASE COUNT         1010 a          974 c          907 g          786 t
ORIGIN      
        1 tatacgaccg ggatgtacgg tattcaccct tcccgacgcg tcaagggtaa acagcctttc
       61 acaaaggaga aaaatcagct atactgattt tcctatgggt aaaacctttg tgtttgtaaa
      121 ccagaaaggc ggggtgggta aaaccacatc cgccatcaat atcggcgcgt atctggccct
      181 ggaagggaaa cgtaccctgt tagtcgactt tgactcgcag ggaaacatga cctccggggt
      241 tggtgttgac cgcgacaaac caacggtcta tgacttgttg gcggggaccg ttacccccaa
      301 ggacgttatc cgggactctg taatacccgg cttgtccgtt atccccgcat ccattgacct
      361 ttcgggcgcg gcgattgaac tggtagacca ggaaaaacgg gaatactttc taaagacagc
      421 actggaagta gttaaagacg agtacgattt tatccttatc gattgccctc cctcgctggg
      481 catcctgacc cttaatggac tggtggccgc caacgaggtg cttattcccc tgcagtgtga
      541 atactttgcc ctggaaggtc ttacgctttt gttgcaaacc gttaaacgag tgcaaaaaac
      601 cctgaacccg gagcttacga ttggcggtat tttctttacc atgtacgatt cccgaacccg
      661 ccttgcccag gatgtagtgc aacaagtaac ttcgtatttt aaggatcgtg tgttcacaac
      721 cattattccc cgcaacgtac gcctttctga agctccgtct cacgggcttc ccatatgtaa
      781 atacgacgcc ctgtgcgtcg gcgcacgcag ctatgaaaaa ctcgccaaag aggtacttga
      841 acgtggctaa gcaaaccgga ctgggccgcg gacttgaggc ccttcttgaa gaatcagaag
      901 acaaagaaca aaccacagta gcaaccgcta aaggcggagc tggaacagag ctgtttatta
      961 gcccaagcct gctcaagcca aatccgcacc aacctcgaca ggaatttgac gaggaagcct
     1021 tacgcgagct tgccgactct atccgcgaac atggcataat ccaaccgatt atcgtagaag
     1081 agaccggaaa cggcggttat tacataatcg ctggagaacg ccgaacaagg gcagcaagac
     1141 tggcaggact taccgaagtt cccgttatcg ttaaaaagtt ttccgaggaa agaaaacttg
     1201 aagttgccct gatagaaaat atccagagag aaaacctgaa tccggtggaa gaagcaaggg
     1261 cgtactatca gctcatgacc ttgggaaacc tgagtcagga agaaactgcg acacgagtgg
     1321 gaaaaaaccg ctccacagtc gctaacgccc ttaggctcct taaactgccc gaagatatcc
     1381 tttcctccct tgcttccgga cagataacgc caggacacgc cagagccatc cttgcggtgc
     1441 taaatcccgc cgatcagaga attctttttg gtcgtattat tggcaacgcc ctttcggtac
     1501 gcgaaacaga gcgcatggcg gcagaactta atggcggcag tcgcgccaca gcaacaaaaa
     1561 aagaaaaaaa gcccgctgac tcttctcgaa gcatggaagt ggctaccctt gagcaaaagt
     1621 ttatagacgc ctttggaaca aaggttaccg taaaaggtag cctggatcgc ggatcaatcg
     1681 agatatcata tttttcgcga gatgatcttg accgtatcta tgaactgatc aatgcctgcg
     1741 gacattaaag tcgcgccggg gttctttctc ctgtaattca ctgcggcgct tctcctccag
     1801 atacaaacgc tcctgaacac gcatgggcgt ttccgaaccg gaaggagaaa gacgagtcca
     1861 ggttccctcg ctggtaaggc gatgagcctt aagattatcc tcaaaataca tacccaataa
     1921 agaccgtatt tctttgcgaa ttgattcttg taatataggg aacatgagct ctacacgccg
     1981 atccagattt ctgggcatcc agtccgcact ggacagaaaa agctcgtccg ctccaccgtt
     2041 ttgaaaccag aatatgcgag aatgttcaag gaagcggtca ataacgctta ccaccgtaat
     2101 cccctcgctc atgcccttaa cgccgggtac cagggtgcaa ataccccgga tattaagcag
     2161 tatttttacc cctgctcttg aggcccgata cagggcatca acgacttccg gatccgcaag
     2221 actattcatc ttggccataa tcatgcccgg cacttcagcc tggcttcgct gagcctcgcg
     2281 atctatcaaa gaaacaaggg tagatttaag atctattggg gccatcttaa ggtacttcat
     2341 ccccataatc gccgagtagc ccgaaatcat attaaagaac agggtggcat cgttggcgat
     2401 tgcagggttt gaggtaaaca ccgaaagatc cgaatagaag cgggcggtac gatcgttata
     2461 gtttccggta gaaagatgca cataccggcg aacgccttcc tgctcgcgtc ttatcaccat
     2521 cagaatcttg gcgtgaacct tgagtcgggc gattccatac acaacgataa cgcccgcttc
     2581 ttcaagccgt tttacccagg taatattgcg ctcttcgtca aatcgcgcct taagctccac
     2641 aaaaacggta acatgcttgc ccgcccgcgc ggcccgctcc agagagcgaa taaccgggga
     2701 gtccccactg gtccggtaca aggtcatttt gatagcaaga acccccggat ccgaggaggc
     2761 atcttccaaa aagcgcagaa ctggctccca ggactgatag ggaacatgaa tcagtaagtc
     2821 ggtagccttg agcgtatccc ataggggtga gtcctctgga aagcgggccg gccaataccg
     2881 gttccaggaa ggatttcgca agtgatcaag accgggagtt tccaccaaat cgattaacgt
     2941 agacaggtca agcggtcccg acacccggta gatatcctgg tctgaaagac ccatccggtc
     3001 taaaagccat gcgctgataa ccggactttc gcccgtacat aaaagcctga cgggacgaga
     3061 agaaagccga ttggccagaa cctcttcgat tgcggctata aagtccccat cacgctcctc
     3121 gtccaccggg ctatccgcat cccgcgttac cttaaaaaga agccgccctt ctatcgtaaa
     3181 gccggggaaa agccgttggg caaaggcaag gactacatcg tccagaaggg taaaaaaccg
     3241 cttgcccgag gcgtcgggta accacacaac acgaggaata ctggatggaa tttgaacaac
     3301 ggcaaaaagc tgccttgctg ccgcaccgga agaatctaca ctatcgccag aaacggagac
     3361 ccctgcaccc tccaaaaaat cggccagggc accttctggt atgtccatgg aagggcgaag
     3421 gtgaaacacc gcatgaagcc tgagattccc gatactcggc aacttaccca gggaagaggt
     3481 ccgtaagggt gttaaaaggg ggaaggcctc gttcataaaa aaggtttcaa gaaagcggga
     3541 ctgtgcctgg gtatactctt cagggcgaac atagctaatg ccctcttttt cgagcccggg
     3601 aaggatatct tgcagtaagc agcgatactg caagggcacc aattcgtgta cgcgccggga
     3661 aataaggcga acctgct
//
LOCUS       DAOQXQ010000085         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26158_length_3671_cov_2.477876, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000085 DAOQXQ010000000
VERSION     DAOQXQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26158_length_3671_cov_2.477876"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1443
                     /gene="tyrS"
                     /locus_tag="PKH81_02160"
     CDS             223..1443
                     /gene="tyrS"
                     /locus_tag="PKH81_02160"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ96871.1"
                     /translation="MNKALETLKERGFFQQCTDVDGLSRLMDDGPVAFYVGFDPTGSS
                     LHIGHMLPAFALRHLRQAGHRAIALIGGGTARIGDPSGKTEMRKMISYEQIDANAEAI
                     AAQLGRFIGFDGKSAFAENNKHWLADLNYIDFLRDIGSHFSVNRMLTFEAYKKRMETG
                     LTFVEFNYQLLQSYDFLQLNERHGVALQIGGDDQWGNIVAGIELIRRINGKQAYGLTF
                     PLITTSDGKKMGKTEKGALFLDPAVTTPYDFFQYWRNVTDADVRRFLLLFTFLPVTDI
                     DTLMAGDINAAKERLAIEVTTLIHGAEEAEKALLGARAAFGGGGDKSSMPTVSIPRER
                     FEAGIGVLDLFYEAGLAATKSDARRLVEQGGAYVGGVNVTSVKDMVSATALDGEGELV
                     VRAGKKKFIRVVSS"
     gene            complement(1433..1822)
                     /locus_tag="PKH81_02165"
     CDS             complement(1433..1822)
                     /locus_tag="PKH81_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNQ96872.1"
                     /translation="MQVALEEIKIRKRVRRKNEDIEPLMDSMKRYGLFNPITINSRMV
                     LVAGARRLEAAKRLGWRTINAVMLDDTDKITELEIELEENVQRSNFTDRELIEAYARL
                     ERMRNPNIFIRIWRAIVAFFHSLFVRN"
     gene            complement(1832..>3671)
                     /locus_tag="PKH81_02170"
     CDS             complement(1832..>3671)
                     /locus_tag="PKH81_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ96873.1"
                     /translation="RDDKARWFSEYVRRQLETMIYGSMDIYSGGYTVHTTLDLRHQAA
                     AEKVMEDYLKTANRRFKSSSAARFAQGDQYAQITELLALSFNLPQLTVSAEQLHVKSI
                     AQYRRTVNPVVDMMSLMFGLDGLKVETNKSNARVQQAAAKTTVEGTLICVDNDTGYIT
                     ALVGGSKFDQSNQIIRATQASVQPGSTFKPLYYSAAIDSRKYTAGSMINDTPVVFFNE
                     SGVPYTPLNFKGEWKGTVLLWEALAHSMNVPSLRILDGIGFDAAINRAASLLGYTNKG
                     DIDKVFPRVYSLGLGVISVAPIQMAKAFAAFANQGKELTPIAIRSVEDRNGKTIIDPE
                     RELRLEQKRKGSAIQLISPQNAYIMTELLKNTIRTGTLGWASGYERKFTFKDKNGRNF
                     VMPAAGKTGTTQNWADAWTVGFTPYYTTAIWFGFDRRGLSLGLDNTGATLAGPAWGDY
                     MHQIHDGLRFRDFVKPQTGLVHATVCKKSGLLATDYCSDGLVTLAYLEGTQPTSYCEY
                     HEKDDELKRIAMDRLQKQSLSVGQRPIETDTSSLVLDPSIFDDPLPGQGKVKQGTPIY
                     QPYNPQGPVITPPQVKKTNEEDEQNQSGDILTDSEEEEPEFNPLLE"
BASE COUNT          842 a          894 c          921 g         1014 t
ORIGIN      
        1 attgataaca ccggaaacac aaccagggag gcaccctgtc cgttcgcgct ttttaccaga
       61 gaaagaatct ctgccgtatt tgctccgcag tctccaaccg tacaactcgg cgttgcagcc
      121 gctaccctga aaaatccgtg cgtcatgttg cgctcctcgc gatttttttg ccaatcaggc
      181 gtagtgtata cttttttttt gaaactcgct atactcgcgc gcatgaacaa ggcacttgaa
      241 acgcttaaag agcgcggttt tttccaacaa tgcacggatg tcgacggtct ttcccgactc
      301 atggacgacg gtcccgtcgc gttttatgtc ggttttgatc cgactggatc gagcctgcac
      361 attggtcata tgcttcctgc ctttgccctg cgccatttgc gccaggcggg tcaccgggca
      421 attgccctca ttggcggcgg taccgcccgc atcggtgatc cttccggcaa aaccgaaatg
      481 cgcaagatga tcagttatga acagattgac gcgaatgccg aagccatagc cgcccagctg
      541 ggtcgcttta tcggttttga cgggaaaagc gcctttgccg agaataacaa acactggctt
      601 gccgatctta actacataga tttcttgcgc gatattggct ctcacttttc ggtaaaccgc
      661 atgcttacct ttgaagcgta taaaaaacgc atggaaaccg gtttgacctt tgttgagttt
      721 aactatcagt tattgcagag ttacgacttt cttcagctta atgaacgcca tggcgttgcc
      781 ctccagattg gcggagacga tcagtggggt aatattgttg cgggtattga gcttattcgc
      841 cgaattaacg gaaaacaggc ttatggactt accttccccc ttattaccac ctctgacggc
      901 aaaaaaatgg gcaagaccga aaagggtgct cttttcctgg atcccgcggt aactactcca
      961 tacgatttct ttcaatattg gcggaacgtt actgatgcgg atgttcgccg cttcctgctt
     1021 ttatttacct ttttaccggt tacggacatt gatactctta tggccgggga tattaacgcc
     1081 gccaaggagc gccttgccat cgaggtgact accctgattc atggcgcaga agaagccgag
     1141 aaggcccttt taggcgcccg tgcggcattt ggcggcggag gcgacaagag ctccatgccc
     1201 actgtatcca taccacgcga acgttttgaa gcaggtattg gtgttcttga tcttttttat
     1261 gaggcaggac ttgccgcaac caagagtgac gcccgccgcc ttgttgaaca aggcggggcc
     1321 tacgtgggcg gggtcaacgt tacctcggtt aaagacatgg ttagcgccac agcccttgat
     1381 ggagaagggg aacttgtagt acgtgccggt aaaaagaagt ttattcgggt ggttagttcc
     1441 tgacaaacaa gctgtgaaag aacgcgacaa tcgcccgcca tatccggatg aatatattgg
     1501 ggtttctcat ccgctccagg cgggcgtacg cttcgattaa ttcacggtcg gtaaaatttg
     1561 acctttgtac gttttcctca agctcaattt caagctcggt tatcttatcc gtatcgtcga
     1621 gcattactgc gttaatcgtt ctccacccaa gccgctttgc tgcctcgagc cgtcttgctc
     1681 cggcaacaag aaccatgcgt gaattgatgg taattggatt aaaaagacca tagcgtttca
     1741 tgctgtccat cagtggttct atatcttcat tttttcgtct tactcgttta cgaattttga
     1801 tctcttcaag tgcaacttgc atgatctcct cctattccag caggggattg aattcaggct
     1861 cttcctcttc actgtcggta agtatatcac ctgattgatt ttgttcatcc tcttcattcg
     1921 tttttttaac ctgtggggga gtgattacag ggccttgcgg attgtacggt tgatatatgg
     1981 gcgtaccctg cttgaccttg ccctgtccag gcagagggtc atcaaagatg gatggatcga
     2041 gtactaaact tgaggtatcg gtttcaatag gcctttgacc cacggaaaga ctttgctttt
     2101 gaagtcggtc catggctata cgctttaatt cgtcatcttt ttcatgatat tcgcaataac
     2161 tggttggctg ggtaccttcc agataggcaa gggtgaccaa tccgtcggaa caataatcgg
     2221 tggcaagtaa tcccgatttt ttacacacgg tggcgtgaac aagacctgtt tgcggtttaa
     2281 caaaatcccg gaagcgaagc ccgtcatgaa tttgatgcat atagtcaccc caggcaggtc
     2341 ctgccagggt cgcgccggta ttgtcaaggc ccaaagacag tccgcgacgg tcaaaaccga
     2401 accagatggc tgttgtgtaa tagggtgtaa atccaacggt ccaggcatcc gcccagtttt
     2461 gggtggttcc cgtttttccc gcagccggca ttacaaagtt ccggccattt ttatccttaa
     2521 aggtaaactt gcgctcatac ccggaagccc agccaagggt tccggtacga atagtgtttt
     2581 taagaagctc ggtcatgatg tacgcgtttt gaggacttat aagttgaatc gcgcttccct
     2641 ttcgtttctg ttcaagacga agttcccttt cgggatctat aatggttttt ccattgcggt
     2701 cttctacaga tctaatcgcg atgggggtaa gctcttttcc ctgattggca aatgctgcaa
     2761 aggcctttgc catctgaatg ggggccacgc tgattacgcc caatcccagg gaatatacgc
     2821 gaggaaagac cttgtctata tctcccttgt ttgtataacc caaaagagag gcagcccggt
     2881 taatggctgc gtcaaacccg ataccatcca gaattcgcaa ggagggtacg ttcatggaat
     2941 gggccagggc ttcccacaaa agtacggtgc ccttccattc tcccttaaaa ttaagcggag
     3001 tgtagggcac accggattcg ttaaagaaga caacgggggt atcgttgatc attgaacctg
     3061 cggtgtattt tcgggaatcg attgccgcgg agtaataaag gggcttaaag gtacttcccg
     3121 gctgaaccga ggcttgggtt gcccggataa tctggtttga ctgatcaaat ttgcttccgc
     3181 ctacaagggc ggtaatgtag ccggtatcgt tgtcgacaca gatcagggtg ccttctaccg
     3241 ttgtttttgc cgctgcttgc tggacccggg cgttagactt gtttgtttcg acttttagac
     3301 cgtcgagccc aaacataagg gacatcatat ccacaaccgg gttaacggtt ctgcggtatt
     3361 gagcgattga tttgacgtga agctgttccg cagatactgt cagttgtgga agattaaaag
     3421 aaagggcgag taattcagtt atttgcgcgt attgatcgcc ctgggcaaag cgtgcggcgc
     3481 ttgacgattt gaaccgacgg ttcgcggttt taagatagtc ttccatcacc ttttccgccg
     3541 cagcctggtg gcgaaggtcc agtgtcgtat gaacggtata accgccggaa tagatatcca
     3601 ttgaaccgta tatcatggtt tccagttggc gacgaacgta ttcagagaac catcttgctt
     3661 tgtcgtcacg g
//
LOCUS       DAOQXQ010000086         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26220_length_3665_cov_2.856510, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000086 DAOQXQ010000000
VERSION     DAOQXQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26220_length_3665_cov_2.856510"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1367
                     /locus_tag="PKH81_02175"
     CDS             186..1367
                     /locus_tag="PKH81_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96874.1"
                     /translation="MTIRDLIQKLSDADLDKKIDVAAWREEKKNNKDFSSTEQAYIRG
                     FICSNNLYGNSITPALPVPDIGSTDIVVLNAQERALVNRVFENLRVYSPETMVGVATR
                     VMDLERLSVSISRYPSMHESGALAGQLRSSETLIETLCAYSDGERLLTLPTKAILGQG
                     FLVAKFHAFAAITKVALNSFFSDQDIQELRQATLNIMFTLMAEDVYMSLLDDPNLRPG
                     VRRDVAESLAELWEHRLDPNVSSLAPVLDAVWTVRDQIAPNFGTMIGTSELLLLSIAL
                     DDTWQRFISSRLGDSDIASSMEEFLFGLSYEDISLIRKELKTRGLSAIGREEVPGIIG
                     HKAALSNEDPRIFYRAYTQRRNNADARKRLQAAGPKKTIEDHYMQFIFEQNRIKKDNG
                     S"
     gene            1357..1935
                     /locus_tag="PKH81_02180"
     CDS             1357..1935
                     /locus_tag="PKH81_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ96875.1"
                     /translation="MAHKKEPFRFGEKLRTVREKKGYTLKVVANAAGVSESLVSQIER
                     NRVSPAIDTLIALADALDIDLEFLFEGYRRERPVRVIRSDERRQVHENEALYEEIVRP
                     VEGDGVHAVESYYITLPPGGETKRGSYGHLGREFGIITEGKATLHYETKEFPLEAGDS
                     ISFSATAPHVLVNTGSGIVKALWVVTPPQRFS"
     gene            2004..3011
                     /locus_tag="PKH81_02185"
     CDS             2004..3011
                     /locus_tag="PKH81_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ96876.1"
                     /translation="MNTKMSLRDELRAISSRTKLPNKDQYMRLLGRTITILEHEDSQI
                     RPADSTGLSGGMVVPDPQLPTLIVPDLHARRDLFFSLLEYPLFGESTVLTALEEGRIT
                     LVCLGDALHAERRALARWLIAWEEYLSGWKKHKAMSEEMIEGLSLIEMVMRCKGAFPN
                     QVHFLKGNHENIMNEEGNGNHAFMKFAHEGEMVRSFLESRMGTDFLQLYALFEKRLPL
                     FVLGNTFLASHAEPARFFTREELIEARNSAEVMLGLTWTANGVADQETVRHMIESYVP
                     DVPGAVYFAGHRPVSGAYAHNQHNNFIQIHNPEGFNLAFVPPDRPFTPEKDIINLAQG
                     V"
     gene            3014..>3665
                     /locus_tag="PKH81_02190"
     CDS             3014..>3665
                     /locus_tag="PKH81_02190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ96877.1"
                     /translation="MADVPEFIGKYKIVSLVARGGMGAVYKAVHPTLKRYVILKKLTI
                     RGNAVITERFKREARILLDFNDPHIVHLFDYFKEGQAHYIVLEYVDGVSLDVLLKKRG
                     SLSGTFALFILRDVCKALKYAHDNGVIHRDIKPGNILISKTGDIKLADFGIASTENSE
                     ADDGLTREGTTLGTVAYMPPEQFSSTKTVDKRADIYAVGVMLYEMVTGVKPFPGSFS"
BASE COUNT         1033 a          840 c          887 g          905 t
ORIGIN      
        1 cgcatggaga aagttcagcc attctttaat gaaggaatca gaaatatcaa tcgatgatgt
       61 gtttgatatt atggaaaagg aactcttgga atcagatctt aagttacata aaaactggcg
      121 gactattaca gtaaaaaatg tttccatttg gtcaaaaacc gattatgaat aacaaggggg
      181 actgagtgac aatccgtgat ttgattcaaa aattatcaga tgcagatttg gataaaaaaa
      241 ttgatgtggc ggcttggcga gaggagaaaa agaataataa agacttttct agtacagagc
      301 aggcatacat cagaggtttt atatgcagca acaatctgta tggtaatagt ataacacctg
      361 ccttacctgt gccggatatt ggctcaacag acattgttgt actcaatgcc caggaacgtg
      421 ctctggtcaa tcgcgttttt gaaaatctgc gcgtatattc tccggaaacc atggtaggag
      481 tagccaccag ggtaatggat ctggagcggc tttcggtatc catatcccgc tatccctcta
      541 tgcacgaaag tggcgccttg gccggtcaac tcagatcctc tgaaaccctt atagaaaccc
      601 tctgcgccta tagcgacggc gagcgcctgt taaccctgcc cacaaaggca atccttggac
      661 aaggatttct ggttgccaaa ttccacgcct ttgcggcaat taccaaagta gcccttaatt
      721 cctttttttc agatcaggat atccaggaac tgagacaggc tacccttaac attatgttca
      781 ccctgatggc agaagacgtg tatatgtctc ttctggacga tcccaacctg cgccccggtg
      841 ttcgccgaga tgtggccgaa tccctggctg aactgtggga acatcgactc gatcccaatg
      901 tttcgagcct ggccccggtt ctggatgcgg tatggacagt tcgagaccag atagcgccaa
      961 actttggaac catgatcggt acgagcgaat tattgctcct gtccattgcc cttgatgaca
     1021 cctggcaacg ctttatttcc agtcgtttgg gagattcaga tatcgcctct tcaatggaag
     1081 aatttctttt tggtctttca tacgaagaca tctcgctcat tcgaaaagaa ctaaaaactc
     1141 gtggattgag cgctatcgga cgcgaagagg ttccaggaat tatcgggcac aaggctgccc
     1201 tttccaacga ggatccacgc attttttatc gggcatatac acagaggcga aacaacgccg
     1261 atgcacgtaa acggctccag gcggccggac caaaaaaaac aatagaagac cattacatgc
     1321 agtttatctt tgaacaaaac cggataaaga aggacaatgg ctcataaaaa ggaacccttc
     1381 cggtttggag aaaaactcag aacggttcgg gaaaaaaaag gatataccct caaggttgtt
     1441 gccaatgccg ctggggtaag cgaaagtctt gtttcccaaa ttgaacggaa ccgggtatca
     1501 cccgcaattg acaccctcat tgcccttgcc gatgccctgg acatcgatct tgagttcctt
     1561 tttgaaggat accgcagaga acgtccggtc agggtaatcc gctctgatga gcggcgacaa
     1621 gtacacgaaa acgaagccct ctacgaagaa attgtcaggc ccgttgaggg tgatggggtt
     1681 catgccgttg aatcatatta cattaccctg ccccctggcg gagaaacgaa acgaggttca
     1741 tacggacact tgggtcgtga gtttggcatt atcaccgaag gaaaagcaac gcttcactat
     1801 gaaaccaagg aattcccgct tgaagccggg gacagtattt ctttttcggc aactgctccc
     1861 catgttcttg ttaacacggg atccggaatc gtaaaggctt tatgggtggt aaccccgcca
     1921 cagagattct cctaatccgt acacagagac gttcggcggc atttttactt cacaagtaac
     1981 ccataccatg agattataga agggtgaata caaaaatgtc gttgcgggat gaattgcgcg
     2041 caatatcttc gcgtacaaag cttcccaata aagatcagta catgcgactc cttggcagga
     2101 ccatcacaat cctggagcac gaagacagcc agattcgccc ggccgattct accggcctgt
     2161 ccggtggaat ggtggtcccc gatccacagt tacccacgct tattgtccct gacttacatg
     2221 cgcgaagaga tcttttcttt tccctgttgg agtatccctt gtttggagag tcaaccgtac
     2281 ttaccgccct tgaagaagga cggattacgc ttgtatgcct tggagacgcg ctccacgcag
     2341 aaaggcgagc cctggctaga tggctcatcg catgggaaga atacctgagt ggctggaaaa
     2401 aacacaaggc tatgagcgag gagatgatag aaggactatc ccttatagaa atggtgatgc
     2461 gctgtaaggg agcatttcca aaccaggtcc actttcttaa gggaaaccat gaaaacatca
     2521 tgaacgagga aggaaacggc aatcatgcgt ttatgaaatt tgcccatgag ggcgagatgg
     2581 tcagaagttt tcttgaatcc cgtatgggta ctgacttttt acagctctat gccctttttg
     2641 agaagcgcct tcccctcttt gtgctcggga atacctttct tgcctcgcac gctgaaccag
     2701 cacgtttttt tacccgtgag gagttaatag aagcaagaaa tagcgcagag gttatgctcg
     2761 ggttaacctg gacggcaaat ggcgtagcag accaagagac tgtacgacat atgatagaat
     2821 cgtatgtacc ggatgtgccg ggggcagtgt attttgccgg acaccggcct gtctcgggcg
     2881 cctatgcaca taatcagcat aataacttta ttcaaataca taacccggag ggctttaatc
     2941 tggcatttgt accgccagat cggccgttta ctccggaaaa agatattatc aatctggcgc
     3001 aaggggtgta gttatggcgg atgtacctga gtttatcgga aagtataaaa tagtgtcgtt
     3061 ggtagcaagg ggtggaatgg gtgctgtcta taaagcggta cacccaaccc tgaaacgcta
     3121 tgtcatacta aaaaaattaa caatcagggg aaacgcggta attaccgaac gttttaagcg
     3181 ggaagcccgt atactgcttg actttaatga cccacacatt gtccacctgt ttgactattt
     3241 taaggaagga caggcccact acatcgtttt ggaatacgtg gacggcgttt cactagatgt
     3301 gttattaaaa aaacggggat ctctttcggg aacctttgcc ttgtttatcc ttcgtgatgt
     3361 atgcaaggcg ctcaaatacg ctcacgacaa tggtgttatc catcgggata taaagcctgg
     3421 gaatatactc atatccaaaa ccggagatat aaagcttgca gactttggaa tcgcttcaac
     3481 agaaaacagc gaagcagatg atggattgac aagggaagga accacattgg gaaccgtggc
     3541 gtatatgccc cccgagcaat tcagcagcac caaaacagta gataaacgtg cagacattta
     3601 cgcagtgggc gttatgctct acgaaatggt aacaggggtt aaaccctttc ccggatcgtt
     3661 tagcc
//
LOCUS       DAOQXQ010000087         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26430_length_3644_cov_2.820841, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000087 DAOQXQ010000000
VERSION     DAOQXQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26430_length_3644_cov_2.820841"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..916
                     /locus_tag="PKH81_02195"
     CDS             185..916
                     /locus_tag="PKH81_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HNQ96878.1"
                     /translation="MKRIIILICFALCAVAPLVAAGIEAITYSNLVSTISRVRSPAVS
                     GTYVVYTASGRARHTGIAFEHENYTQVHSFQRLIRKDEFGKPRRTESGKLEDTILFYI
                     AQIPPQTSAIRYRIVVDGLWTADPENPLQEWDAERGFSVSVLEVDSYEVFKTSNVQSN
                     TVRFTCIAPSGQKVSLAGSFNNWDPFMYEMTETSAGMYELTLPLPRGTWYYAYFQGTT
                     QVPDTTNSDRAYTRDGRVASVITIP"
     gene            complement(931..2400)
                     /locus_tag="PKH81_02200"
     CDS             complement(931..2400)
                     /locus_tag="PKH81_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96879.1"
                     /translation="MTHKTLYGNKIPAKILERAEARFSRYAKKFSFDPGLSPDLVLCP
                     EGQGFEDFGILTVREKKEGLACQQGFDRDNGIMLGTIRMGFGHCRMAIALASASHSMG
                     YTPYWLDLMSFPDYAASKTIKYLEDLYNLGSRISQRSRLFNSLVWEYVTSGAARKLSF
                     SVEEDILSRIFVPLYRDIPKDLPFFSTHPWTGHAAVGAGMSDVVTIIPDNYPLAFHVV
                     EGSVHAVQSPSSYMGYRTFHSMGSGLRLDHTMPSKDIEMVGHYIDHEIVAGIEEDCAS
                     RRRRIKDKETRRFLLTMGGAGAQVLRFADIALTCKEAIEDTKVALLINMGDHRGRWKE
                     LESLFTQQGIRWTLHQDWSETRAFIDEARDCPISGVHVFLHDDFYCAVYATNLLMRIA
                     DVMITKPSELSFYPVPKLFIQRVGRHEAWGAIRGAEIGDGTIETASVTGLHRLLKLLV
                     EDDDLLDLYCTHILVNKKNGVYNGAYNAVKLAVKNRKRE"
     gene            2463..3410
                     /locus_tag="PKH81_02205"
     CDS             2463..3410
                     /locus_tag="PKH81_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNQ96880.1"
                     /translation="MRQDTYDGLMKAERLLAILTVLMDKKMVSASFLASRLEVSIRTI
                     YRDIDALSAAGIPVFATTGRDGGFALVDGFKMSDSVLATGEIQHILTGLSGLAAIYQG
                     PTLTIITDKLRTLLESSRQKGVPCPENHIFIELVPSKRDKKSIDSLRQSIEKSLVVSI
                     TYTDSSNQETIREIEPCALIYLWQSWYTWAWCRLRKDFRLFRITRIRDITILPYERES
                     EPQDLSSKPWARQWETNPFSQVCFRAPKASLGRLYECFDDDYIGAVDSQTVQVSANIP
                     IDEWFISWIMGLPGPVTILEPESLAEAVQLRAKSFLEQR"
     gene            3486..>3644
                     /locus_tag="PKH81_02210"
     CDS             3486..>3644
                     /locus_tag="PKH81_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNQ96881.1"
                     /translation="MIAFCGLDCASCGAYIATMTNDNAMREKIAGEWKVAYNFDFTPQ
                     MINCHGCRA"
BASE COUNT          962 a          929 c          827 g          926 t
ORIGIN      
        1 agttcgagtt cttttctccc cggcatttgt ttcctgtacg ttcggtattc agtatatcat
       61 cggtttttaa tgacggtact ttaccgggat gatgcatata caagcgttgt ttcgccgata
      121 gtagctttcc acacggtata cctcaagatt ttgaaggatt ttccgacaat tgtaaagaag
      181 cactatgaaa cgtattatca ttctgatttg tttcgcactg tgcgccgtcg ctccccttgt
      241 ggcggcaggc atcgaagcga ttacctattc aaacttggtc tcaacaatat cccgggtccg
      301 ctctcccgcc gtttcgggaa cctatgtggt atacaccgct tctggtcgcg cccgccatac
      361 cggtatcgcc tttgaacacg aaaactatac acaagtccat tcatttcagc ggctaatccg
      421 aaaagacgaa ttcggcaaac cgcgaagaac cgaatccgga aaactggaag acaccatcct
      481 tttttacatc gctcagatac caccacagac cagcgccatc cgctaccgta tcgttgtaga
      541 cggcttatgg actgccgatc cggaaaatcc ccttcaggaa tgggatgccg aacggggctt
      601 ttcggtttct gtactcgagg tagactccta cgaagtgttt aaaacctcaa acgttcaaag
      661 caataccgtc cggtttacct gcatagcccc aagcggtcaa aaagtaagtc tggccggcag
      721 ctttaataac tgggatccct ttatgtacga aatgaccgaa acctcggcgg ggatgtatga
      781 actcacgctt ccccttcccc gcggtacctg gtattacgcg tactttcagg gaaccacaca
      841 agtcccagat accactaact cagaccgtgc atacaccagg gatggcaggg tggcctcggt
      901 aattactatt ccctgacttt tttgtctctc ttattcccgc ttacgatttt ttaccgcaag
      961 tttaaccgca ttataggcac cgttatacac accatttttc ttgtttacca aaatatgagt
     1021 acaatacaag tccagtaaat cgtcatcctc aacaagaagt tttaacaggc gatgtaaacc
     1081 ggtaaccgaa gcggtttcga ttgtaccgtc gccaatctcg gccccgcgaa tcgcccccca
     1141 ggcttcatgt ctgcccaccc gttgaataaa gagcttgggt accggataaa aggacagttc
     1201 gctcggtttt gtaatcatca catcggcaat tcgcatcaat agattcgtcg cgtagactgc
     1261 acagtaaaaa tcatcatgga ggaatacatg aaccccggag attggacagt cacgggcctc
     1321 gtcgataaac gcgcgagttt cactccaatc ctgatgcaag gtccatctga tgccttgttg
     1381 cgtaaataat gattccagtt ccttccatcg cccgcgatga tcacccatgt tgatcagtaa
     1441 ggcaaccttt gtatcttcta tggcttcttt acaggtaagc gcaatatcag caaatcgaag
     1501 aacctgggct cccgcccctc ccatcgtgag caaaaaccgg cgtgtctctt tatccttgat
     1561 gcgccgtctg cgagatgcgc agtcttcttc tatgcccgcc acaatttcat gatcgatata
     1621 gtgacccacc atctcgatat ccttacttgg catggtatgg tccaatcgca aaccgcttcc
     1681 cattgaatga aaggttcgat accccatgta acttgaggga gactgaacgg catgtacact
     1741 tccttcaacc acatgaaaag caaggggata attatcggga ataatcgtca ctacatcgct
     1801 catcccggct ccaacggcgg cgtgtcccgt ccacggatgg gtagaaaaga agggaaggtc
     1861 cttgggtata tcccggtaca ggggaacaaa gatacgggaa agaatatctt cctccacgga
     1921 aaaagagagc ttacgggcag ccccacttgt tacatactcc cataccagag aattaaaaag
     1981 gcgggatcgt tgggaaatgc gcgagcccag attatataga tcctcaaggt atttgattgt
     2041 cttgcttgcg gcataatcag gaaaggacat aagatcaagc caataagggg tatagcccat
     2101 tgaatgagat gcggaggcaa gtgcaatggc cattcgacag tgtccaaaac ccatacgaat
     2161 agtgccgagc attattccat tatcccggtc aaatccctgc tgacaggcaa ggccttcttt
     2221 tttttcgcgc accgtcagaa ttccaaaatc ctcaaatcct tgaccctccg ggcaaaggac
     2281 taaatctggc gaaagccccg ggtcaaatga aaactttttg gcatagcgtg aaaaacgagc
     2341 ttctgctcgt tccagaatct tggcgggtat tttattaccg tagagtgttt tatgcgtcat
     2401 ttgtactcat tctaccccag cttacgcaca tacgccataa aaaaacgaca gtgcttgccc
     2461 gtatgcgtca agacacgtat gatggactga tgaaagccga gcggctactg gcaatcctga
     2521 cggtacttat ggataaaaaa atggtaagcg cctctttcct tgcctctcgt ctggaggtat
     2581 ccatacgtac tatctaccgt gatattgacg ctctgtccgc ggcaggaatt cctgtttttg
     2641 cgaccaccgg gcgtgatggt ggatttgccc tggtggacgg attcaagatg agcgacagtg
     2701 ttttagcaac cggagagata cagcatatcc tgacaggtct ttctggcctt gccgcgatct
     2761 accagggtcc aacccttacc ataattaccg acaaactacg aacccttctt gaatcatcca
     2821 ggcaaaaagg ggttccttgc ccggagaatc atatctttat agaactggtt ccctccaaac
     2881 gggataaaaa aagcatcgat agtctgcgcc aatcgataga aaaaagcctg gttgtttcta
     2941 ttacctatac tgactcctca aatcaggaaa caatcaggga gatagaaccc tgtgctctta
     3001 tttacctgtg gcagtcatgg tatacctggg cctggtgtcg gctacgaaag gatttcaggc
     3061 tattcaggat tacacgtatt cgagatataa cgattttacc atacgagcga gaaagcgaac
     3121 cacaggatct ttcttccaaa ccctgggccc gccaatggga aaccaacccg tttagtcagg
     3181 tatgcttcag agcgccaaag gcctcgcttg gccggctgta tgagtgcttt gatgacgact
     3241 acataggcgc agtagattca caaacagtac aagtttccgc aaacatcccg attgacgagt
     3301 ggtttatctc ctggattatg ggacttcccg gtcctgttac tatacttgaa cctgaaagcc
     3361 ttgcggaggc ggtgcagctt agggctaaaa gtttcttgga acagaggtaa agactgacac
     3421 gagatgtcag tctttacatt ctatactgtc tctatcacgt actgcggagg attagtatgg
     3481 aaaagatgat cgcgttttgc ggccttgact gcgcttcttg cggagcctat atcgccacaa
     3541 tgaccaatga caacgccatg agggagaaga tcgccgggga atggaaggtg gcgtataact
     3601 ttgattttac cccccagatg atcaactgtc acggatgcag ggca
//
LOCUS       DAOQXQ010000088         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26608_length_3627_cov_3.766517, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000088 DAOQXQ010000000
VERSION     DAOQXQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26608_length_3627_cov_3.766517"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKH81_02215"
     CDS             complement(<1..319)
                     /locus_tag="PKH81_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNQ96882.1"
                     /translation="MSENQFMETFREEAFELLGTLESNLLELEERPDDTELLSAVFRV
                     MHTIKGSSAMFGFDRISAFSHEVESILSALRDGKIAVTAELIGNTLVSRDMILEMLEG
                     TVES"
     gene            complement(337..651)
                     /locus_tag="PKH81_02220"
     CDS             complement(337..651)
                     /locus_tag="PKH81_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ96883.1"
                     /translation="MPKSTVLSFPSVTIGSVSTDRLALLKAFDSGDPVVVDVSATETI
                     DLAGFQLLVALVKEGIKRGVPVTLTGQIPVSLQEKIKKVGLSDTDCTRGEDLYEQVSL
                     DL"
     gene            complement(644..1156)
                     /locus_tag="PKH81_02225"
     CDS             complement(644..1156)
                     /locus_tag="PKH81_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNQ96884.1"
                     /translation="MHSDTIESTEITQYLTFKLADEIYAINVGHIREVLSVPRITRVP
                     RMPSYLSGVINLRGNVIPVLDIREKFGMGKTTITGNTSIIVTEIEEVFVEEADSSMVI
                     GIFSDEVQKVIDLDPSLIEGPPRIGSTIDTSFIEGMGKTDEGFVILLRLDRILSDTEL
                     SGITVEARDA"
     gene            complement(1149..1688)
                     /locus_tag="PKH81_02230"
     CDS             complement(1149..1688)
                     /locus_tag="PKH81_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNQ96885.1"
                     /translation="MVNKAEIRQYLTFKLADEQYAINVANIKEVLGIPKITKVPRMPP
                     FMTGIINLRGSVVPILDLKKKFALGETIIGKETGIIVIELAGKDGEGNDEQLVVGIFS
                     DEVQKVITIEESEIEGPPQIGIAIDTAFIQGMGHVDGGFIIILNIDKILTDSEIHAIA
                     EQGDQSELAMENTVEGTDA"
     gene            complement(1715..>3627)
                     /locus_tag="PKH81_02235"
     CDS             complement(1715..>3627)
                     /locus_tag="PKH81_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ96886.1"
                     /translation="RDKAYWDTVIKHMDEITRTAEVLRDADSDTKVKEAYQGIADGSR
                     DYKKYFSEYAEYEGQRAQLETKLADKGIEVEAAISAVYDRQEVDFAALVARRAGSVEL
                     TDKVDKIDRSGGMIRNLLHARINLNKYILYGDKGSLENVEKEIGSMVEQARFLLPQFR
                     QRANIDAMNLVIAGAAEYQREINDYTDLQARQRAVQQVAAEAGAKTVRLAMEISNTVA
                     SEMDTLMAATTAFILVCSLVSIVVGIILSIVITRLITRPIAVAVRQAEEIAGGNLTRD
                     LPKEYRGRSDEIGDLARALQDMMDRLRQIISEIQSAVLQVSSGSQELSGTAQQMSQGA
                     TEQASSVEEISASMEQMTANIRQNAENALQTEKIAQKSAVSAEEGGKAVGATVAAMKE
                     IASKIGIIEEIARSTNMLALNASIEAARAGEYGKGFAVVAAEVGKLAERSQKEAGEIS
                     TLSSESVTIAEDAGSTISALIPEIKRTAELVQEISASSNEQNSGAGQINSAILQLDSV
                     VQQNASASEQSASMSEELASQAEQMQSAMAFFHLAQDNEANKPPIAHKKIPQSVVSKA
                     IPNKGVLRPDTPVIKKAPEAIAAPEKKAPKQSTKETKTPKRTPLTGIHLDLDSDNPTG
                     GRDDLDSEFQEF"
BASE COUNT          907 a          950 c          730 g         1040 t
ORIGIN      
        1 cgctttcaac cgtaccttcc aacatttcca ggatcatatc acgcgataca agtgtattac
       61 ctatcaactc tgcggtaact gcaatctttc catcacgcaa agcagaaaga attgattcaa
      121 cctcatgact aaacgcggag atacggtcaa acccaaacat ggcggaagaa cccttaatgg
      181 tatgcattac gcggaaaact gcagaaagca attctgtatc atctggtcgc tcttcgagct
      241 caagaagatt tgattccaat gtgcccaaaa gctcaaaggc ttcttctcga aaagtttcca
      301 taaattgatt ttctgacatt tcttctcctt tatggattac agatccagtg aaacctgttc
      361 atataaatct tcgcctctgg tacagtccgt atccgaaagc cctacttttt taatcttttc
      421 ctgaagggaa accggtatct gtcccgtcaa ggttaccggc acaccacgct ttatgccctc
      481 cttaaccagg gcaactaaaa gctgaaaacc ggcaaggtca attgtttccg tcgcagacac
      541 atcaacaaca acaggatctc cactatcaaa ggctttaaga agagcaagac gatcagtaga
      601 aaccgaacca atggttacag aaggaaaaga aagcacagtg cttttaggca tcgcgagcct
      661 ccacggtaat tcccgaaagt tcagtatccg acaggatgcg atcaagccgg agaagaatta
      721 caaagccttc atcggttttt cccatcccct ctataaacga tgtatctatc gtcgatccta
      781 tccggggagg cccttctata agagatggat caagatctat taccttctgg acttcatcgc
      841 taaaaattcc tatcaccatc gagctgtccg cttcttccac aaacacttct tctatctcgg
      901 ttacaattat acttgtattg cctgtaattg tagtcttgcc catgccgaat ttttcgcgta
      961 tatccaatac aggaattaca tttcctctca ggtttattac gccgcttaaa tacgagggca
     1021 tccgcgggac gcgggttata cgcggcacag aaagaacttc cctaatgtgg ccaacgttaa
     1081 ttgcgtatat ctcgtcagcc agtttaaagg tcagatactg ggttatctcg gtgctttcga
     1141 ttgtatcgct atgcatccgt tccctccaca gtattttcca tggccaactc cgattgatcg
     1201 ccttgttcgg ctatggcatg aatctcgctg tcggtaagga ttttatcgat atttagaata
     1261 ataataaaac cgccatcaac atgtcccata ccctgaataa atgcggtatc aatcgcaata
     1321 ccaatctgcg gggggccctc tatttcggat tcttcgatgg taattacctt ctgcacctcg
     1381 tcgctaaaaa taccaactac caattgctcg tcattcccct ctccatcctt cccagcaagc
     1441 tcaatgacga taatgccagt ttcttttccg ataattgttt ctcccaacgc gaacttcttt
     1501 tttaaatcaa ggataggaac aacagatcca cgaaggttta ttataccagt cataaatgga
     1561 ggcatacggg gaaccttggt tatcttggga atccccagaa cctcttttat attggccacg
     1621 ttaatagcat attgctcgtc cgcaagcttg aatgttaagt actgacgaat ctccgcttta
     1681 tttaccataa caataccccc tgccaaaata tcaattagaa ctcttgaaac tcactatcaa
     1741 gatcatcccg tccacccgta ggattatcgc tatccaaatc aagatgaatg ccggttaagg
     1801 gtgttcgttt cggcgtctta gtctcttttg tggattgctt gggtgctttt ttttccggag
     1861 cagcgattgc ctccggagct ttctttataa caggggtatc cgggcgaagg acgcccttat
     1921 tcggtattgc cttggacaca accgattgcg gtattttttt atgcgctatg gggggcttat
     1981 tcgcttcgtt atcttgtgca agatgaaaaa aagccatcgc cgattgcatt tgctccgcct
     2041 gacttgcaag ttcctcggac atagaggcgg actgttcgga ggcagaagca ttttgctgta
     2101 ccacagagtc aagctggagg atggctgagt taatttgtcc tgcaccagag ttctgttcat
     2161 tgcttgacgc gctaatttcc tgtacaagtt cagccgttcg tttaatctcc ggaattagag
     2221 ccgaaatagt ggaccccgca tcttctgcta tggttacact ctcggaagac agagtgctga
     2281 tttctcctgc ctctttttgg cttcgctcgg cgagttttcc aacctctgca gcaacaaccg
     2341 caaaaccctt tccgtattca ccggcacggg ctgcctctat cgacgcgtta agtgcaagca
     2401 tgttagttga acgagctatt tcctcgataa tgccaatctt gctcgcaatc tccttcatcg
     2461 cagccactgt tgcgccaaca gcctttccac cttcttccgc cgatacagcg gacttctgcg
     2521 ctatcttttc tgtttgcagg gcgttttcgg cattttgcct gatattcgcc gtcatctgtt
     2581 ccatggaagc cgaaatctct tctaccgaag aagcttgttc tgttgcacct tgactcatct
     2641 gctgggcggt accacttaat tcctgacttc cggatgatac ctgtaacacc gcggattgaa
     2701 tctcgctaat aatctgtcta aggcgatcca tcatatcttg gagagcacga gccagatcac
     2761 caatttcatc acttcgacca cgatattcct tgggtaaatc ccgggtaaga tttccgccgg
     2821 ctatctcttc tgcctgacga accgccactg ctataggtct ggtgatgagt cgggtaatga
     2881 caatggacag aatgatgcca accacgatag agacaagcga acaaaccagg ataaatgcgg
     2941 tggttgcagc catcagggtg tccatttcgg aggccacagt attactaatc tccatggcaa
     3001 gccgaactgt ttttgccccc gcctctgcgg caacttgctg taccgcacgc tgacgggctt
     3061 gcaaatcggt ataatcatta atttctcttt gatattccgc cgcaccggca ataacaaggt
     3121 tcatcgcatc tatgttagcc cgttgccgga actgcggtaa cagaaaacga gcttgctcta
     3181 ccatagagcc tatttccttt tctacattct caagagaacc cttgtccccg tataaaatat
     3241 atttgtttaa atttatccgg gcatgaagca gattgcggat cattcctccg ctccgatcaa
     3301 tcttatctac tttatcagtc aattcgaccg atccggctcg gcgcgcaaca agagcagcaa
     3361 aatccacttc ttgccggtca tagaccgcag atatcgccgc ttctacctct attcctttgt
     3421 ccgctagttt tgtttccagc tgtgccctct gtccctcata ttcagcatac tcggagaaat
     3481 atttcttgta gtcgcgggag ccatctgcaa tcccttgata agcttcttta actttagtat
     3541 ctgaatcggc atcccgaagc acctcggcgg tacgggtaat ttcgtccatg tgctttatta
     3601 cggtatccca atacgccttg tctcgat
//
LOCUS       DAOQXQ010000089         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26705_length_3618_cov_2.259613, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000089 DAOQXQ010000000
VERSION     DAOQXQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26705_length_3618_cov_2.259613"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PKH81_02240"
     CDS             <1..161
                     /locus_tag="PKH81_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96887.1"
                     /translation="DPLYGVRPLKRAIQSELENPLAKEVLAGNFPDGSIIRVFREKGS
                     EGLSFIKG"
     gene            202..1356
                     /locus_tag="PKH81_02245"
     CDS             202..1356
                     /locus_tag="PKH81_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNQ96888.1"
                     /translation="MDMHTGTLYKSLGFPSVTVHETNGHFDFVEPSRTILVIGPMGSG
                     KTEYAARLWRDAAVARRKGSSIAWLTSGGGNQKELFEPESGIGSADRRNTFFVRYSLD
                     KTRFADYPDDALAYRGGYERCGNNIATISSSFDLEELIRHNPGIGTWIIDEAAFYDER
                     LAYLVKRESEQRGLVFVMPTLLLNFRGELFNATARLLMETSTDIYPLSAYCEHRECLE
                     NAHNTYRYYLVDGQECPALFFDPLIIVGGDRASDNPAEPNYCTRCDSHHYLPGKHYTY
                     FTLKPLGQRASIGDMSALEKELRAIKENSDSSELVRSFRSTYLESGSPSSEHMNALKV
                     PFIAERAVTYLFAEQNLLSAEQTRALVDGLDLNREYLSRRLSDNKRPLAL"
     gene            1384..>3618
                     /locus_tag="PKH81_02250"
     CDS             1384..>3618
                     /locus_tag="PKH81_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96889.1"
                     /translation="MRVRTLLYVMLLGILLPLSAQIDFAPIDTHAYTWLKIDTEHFEV
                     IFPDFLEEQGRYTAAALEYSRQFQLHSLSPHRTHRFPVVLNPDHMSPNGYVSILPRRS
                     FFYTTDNLEDAEDWLTQLAIHEGRHMVQFEAYNLGSVHAASFFFGEYAALLAFMAPVW
                     WLEGDAVMAETVLSQSGRGRRPSFTAQIKALFLEGKVFDYSKMVLGSSVDYTPSPYEF
                     GYLMYAHLRSTYDFLAPQILVSRLARLPLPVIGPHAAIRRVSGKSAAEVYREAATRYT
                     RFWEEQTASFQYTDRHVYSTPTKASYKKHIDLTRAPDGTLFSAIYDREAGYWIVEVSD
                     QKETRVCRAYPQNSLSAGGQYLIWDALSPKAKFDGSGMNIVLYDRATRSRTTLFSRSR
                     FRSPTLSPDGTKAVLVEDRLDGQKVLTVISIPDGSVLDTVVIPSGQRWSSFSFTSEGD
                     ALVMMVYSNGRDGQGEGLRIEQLSLKTGSREQLYIGHQEGLSSPVLEDGVLYFVSDRS
                     GIDAIWARTPDGRLYQAVSRPIGAYSPCIAPEGLFFVDYASSRGTVVARSSIDQEQWL
                     SESEVTPYRENFFEPVADQDPGREKVLPEHVPDITAEAQPYTPFFSGNCIDSWGIVPV
                     SEGDIGWMAFIHSAHTTGLMDQTLSAFWDATDETFGGGWSYRYRGFYPDIVLNVRGEF
                     PRDDSIQYSEYQAVASLEFPYSFGMLGSSILAGAVGFSAGGLLRDSNVEPVYVAYGNS
                     AYSRG"
BASE COUNT          793 a          878 c          924 g         1023 t
ORIGIN      
        1 atgatcccct gtatggagtg aggcccctta agcgggcgat tcagtcggaa ctggaaaatc
       61 cgttggcaaa agaagtcctt gcagggaatt ttcctgacgg ctctattata cgggtgtttc
      121 gggaaaaggg ctccgagggt ctttccttta taaaagggta agttttccct ttcgggtaaa
      181 gttgtggtat actgctccgg tatggacatg cataccggca ccctctacaa aagtcttggc
      241 tttccttcgg ttaccgttca tgaaacgaat ggtcattttg attttgttga accttctcgt
      301 accatcctgg ttatcggtcc catgggttca ggtaaaacgg aatatgcggc tcgcttatgg
      361 cgcgatgccg cggtagcccg acgtaagggc tcttcaattg cctggctcac cagtggcgga
      421 ggaaatcaga aagagctctt tgaacccgaa agcggcatcg ggagcgcaga tcgcagaaat
      481 accttttttg tacgatatag tctagataaa acccgattcg ccgattatcc cgacgacgcc
      541 cttgcctatc gtggtggcta tgagcgttgt ggcaacaata ttgccactat ttcaagttcc
      601 tttgatttag aagagttgat tcgccacaat ccagggatag gaacatggat aatcgatgag
      661 gcggcttttt atgatgagcg cttagcgtat ctggttaaac gggaatctga gcagcgcggc
      721 ctggtctttg ttatgcccac cctcttactg aatttccggg gagaactgtt caatgcaacg
      781 gctcgtctgt taatggaaac ttcgaccgat atctatcccc tttctgccta ttgcgaacat
      841 cgggaatgtt tggagaacgc tcataacacc tatcggtact atctggtgga tggtcaggag
      901 tgtccggcct tgttttttga tcctttgatt attgtggggg gagaccgtgc ttccgataat
      961 cctgctgaac cgaattattg cactcgatgc gattctcatc attatttacc cggaaagcac
     1021 tatacctact ttaccttaaa acccctggga cagcgagcga gtattgggga tatgtctgct
     1081 ctggaaaaag agctacgggc aataaaggaa aacagtgatt caagcgagct tgtccgttcg
     1141 tttagatcca cgtatcttga aagtggcagt ccctcctcgg agcatatgaa tgcccttaag
     1201 gtgcccttta tcgccgagcg ggcggtaacc tatttatttg ctgaacaaaa ccttctctct
     1261 gcagaacaaa cccgagcctt ggttgatgga cttgatttaa acagggagta tctttcgcgc
     1321 aggctttcgg acaacaagcg tccccttgcc ctatagtgtg taccccggcg ccggaggaat
     1381 cgtatgcgtg tcagaactct tctttatgtt atgctcttgg gtattctctt gcccctttcg
     1441 gctcaaatcg atttcgctcc gattgatacc cacgcgtata cctggctcaa gattgacaca
     1501 gagcattttg aggttatctt tccggatttt cttgaagaac aggggcgcta tactgccgcg
     1561 gccctggagt attcccgtca atttcagcta cacagtcttt ctccccaccg tacccatcgc
     1621 tttccggttg ttttaaaccc ggatcacatg agccccaatg gatacgtctc gattcttccc
     1681 agacgaagct ttttttatac cactgataat ttggaagatg ccgaagactg gttaacccaa
     1741 ctggcgattc acgagggccg gcatatggtt caatttgagg cctataactt gggttctgtc
     1801 catgctgctt ccttcttttt tggggagtat gcagcccttc tagcttttat ggctccggtt
     1861 tggtggcttg agggtgacgc tgttatggca gagacggtgt tatctcagtc gggaaggggc
     1921 cgaaggcctt cgtttaccgc tcaaatcaag gccctttttt tggaaggtaa ggtctttgac
     1981 tattccaaga tggtgctggg ttcctctgtt gattataccc cctcgcccta tgaattcggt
     2041 tatttgatgt atgcacatct acgatctacc tatgattttt tggctccgca gattcttgtt
     2101 tcccgacttg cgcgccttcc cttgcctgta attggccccc atgcggcaat tcgccgggta
     2161 agcggaaaaa gcgctgccga ggtatatcgg gaagctgcta cccgttacac ccgtttttgg
     2221 gaggaacaga cagcctcctt tcaatatacc gaccggcatg tatactccac acccacaaaa
     2281 gcctcttaca aaaaacatat cgaccttacc cgcgctcccg acggaacact tttttcggcc
     2341 atctatgatc gtgaagctgg ctactggatt gtcgaggtgt ccgatcaaaa agagacccgt
     2401 gtgtgtcgcg cgtatccgca aaattcctta agtgccggcg gacagtatct tatatgggat
     2461 gccctgagtc ccaaagccaa gtttgacgga tcgggcatga acatcgtctt atatgatcga
     2521 gcgacccgta gccgtacaac tttattcagt cgctctcgtt ttcgtagccc aaccctctct
     2581 cctgatggaa caaaggcggt tcttgttgaa gacaggctcg atgggcagaa ggtcttgacg
     2641 gtgatatcaa ttcccgacgg aagtgttctt gatactgttg taatcccatc aggtcagcgc
     2701 tggtcatctt tttcctttac aagcgaggga gatgcgcttg ttatgatggt ttattcgaat
     2761 ggacgagacg gacaagggga ggggctgcgt attgaacagc tttcgctgaa aaccggctcc
     2821 cgcgagcaat tgtatatagg tcatcaggaa gggctttctt ctccagttct ggaagatggc
     2881 gtgttgtact ttgtctctga ccgcagcggc atcgatgcaa tctgggctcg tacgccggat
     2941 ggaaggctgt atcaggcggt atcccgtcca ataggtgcgt acagtccctg tatcgctcca
     3001 gagggactat tctttgtcga ttatgcaagc tcgcgaggaa cggttgttgc ccgctcctcg
     3061 attgatcagg aacagtggct ctctgagtct gaggtaaccc cgtatcgaga gaattttttt
     3121 gagcctgttg ccgatcaaga tcctggcagg gaaaaggtct tacctgaaca tgttcctgac
     3181 atcacagctg aggcccagcc ctatacgccc tttttctccg ggaattgtat tgattcctgg
     3241 gggattgttc cggtaagcga gggagatatc ggttggatgg cctttattca ttctgctcat
     3301 acgactggcc tgatggacca gaccctttcg gcattttggg acgcaaccga cgagaccttt
     3361 ggcggagggt ggtcttatag gtatcgcggg ttctatcctg atatagtcct gaatgtgcgg
     3421 ggagaatttc cccgagacga ttcgatacaa tatagcgaat atcaagccgt agcttctctt
     3481 gaatttccgt attcctttgg tatgcttggc tcttcaatcc tggcaggggc tgtaggcttt
     3541 tctgccgggg gattgcttcg cgattccaat gtggaaccgg tctatgtcgc gtacgggaat
     3601 tccgcctaca gtcgggga
//
LOCUS       DAOQXQ010000090         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_26906_length_3599_cov_2.567438, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000090 DAOQXQ010000000
VERSION     DAOQXQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26906_length_3599_cov_2.567438"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PKH81_02255"
     CDS             complement(<1..1232)
                     /locus_tag="PKH81_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ96890.1"
                     /translation="MDLTFILNTTFALAVLAGFTLANMRIVHKTSLQWRLFALGLSFF
                     TSISIFSLMHLSILASVISIQFAFVLSCWVSGILYLSFLVDMTSIRRSRYFFISYLST
                     IPLSVISLMLPRPGGVYAIIAVATSLVFGYSWSFLSTWIRSATDDRARRDGEWIALVF
                     VAYGLGLLICFFHALTGFFWVLVLWYGTVFYTVNHLGLFRNLTNRENQLILDNVFDVV
                     IILDTSGHILRMNRRGFQITEFPVIELTGNGIETVIKHPELTARNRRLWLEQHGWLDT
                     GINTRRSPSIDAVIKTRFDEEIPVDLRVVCLVNLAREKTGYIVSASDMRITRQLMKEI
                     TDREYAARDLALSENKFSRMFIFNPVGIMIVDLETMEITDANPAIEEIFGCETSCIVR
                     KKLFDIGLEMDALPYAVF"
     gene            1284..2513
                     /locus_tag="PKH81_02260"
     CDS             1284..2513
                     /locus_tag="PKH81_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNQ96891.1"
                     /translation="MKIWIKYLAGVLIGLAFSLVAPHQNPIFSDITAYLSALVVQFGR
                     YSLYPVLFFSFTIGVFELRESKSLMKIGSIAAAVIIIATILAAVTGLLSVLIRNPSRI
                     PIFVEGASEIGDIGIKESLMQLFPSSGFEVFTNGLFILPLCIFGGFAGAGCAVDKTAS
                     KAALTLFDSLSRVFYAIMSFFVDILSIGMIALSVHWLLEFRLMISSKVFTDFILLLAF
                     NFFFLALVLYPALIRFVCGKVNPYRVLYAGIASILAAFFSGDSNMTLPILLRHANESL
                     GVRRRVSSVTMPMFSIFSRPGTAMAVTVSFIVILKSYSSLGIVFTDMLWLLGVAILLS
                     FFLGRFPSGGAYIALATVCMLYGRGFEAGYLILKPAAFFICSIAAATDALTSLMGTYA
                     MAKRWDMVHHRELRFFI"
     gene            complement(2519..>3599)
                     /locus_tag="PKH81_02265"
     CDS             complement(2519..>3599)
                     /locus_tag="PKH81_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNQ96892.1"
                     /translation="FREIIENDSPQAKKMEIPVVLGKDITGEAQLLDLAATPHLLIAG
                     STGSGKSVCVNSIILSILYKRSPEDVKLILIDPKIVELKLYNDIGHLLAPVITEPKKA
                     FQSLQYCLCEMERRYALLDGMGVRDIRSYNHRIKERKIATEKIPYLVVIIDEFADLMA
                     TTGKELESTVARLCAMSRAVGIHLVLATQRPSIDVITGLIKANIPTRIAFMVASKMDS
                     RIIIDQVGAEKLLGRGDMLYASATDPFPVRIQGAYVSEEEVERVVEHVKTLGEPEYID
                     EEIFVDDEDDSPIDASLFSDGDDPLYDKALEIVMLAGKASASYIQRKLKIGYNRAARL
                     VEEMEQRGIVGPANGSKPRDVIHHV"
BASE COUNT          929 a          940 c          807 g          923 t
ORIGIN      
        1 aaaaacaccg catacggaag agcatccatc tccaacccaa tgtcgaacag tttttttctt
       61 acgatacaac tcgtctcaca gccaaatatc tcttcaatcg ccggatttgc atcggtaatc
      121 tccatcgttt ccaaatctac gatcatgatg ccaacgggat taaagataaa catacgggaa
      181 aacttattct cggacagggc aagatcacgc gcggcatact cgcggtcggt aatctccttc
      241 atcagctgcc gggtaatgcg catgtcgctc gccgacacaa tgtagcctgt tttttcccgg
      301 gcaagattta ccaaacacac aaccctaagg tctaccggta tttcttcgtc aaaccgtgtt
      361 ttaataaccg cgtcgataga agggcttcgc cgtgtattga tacccgtatc aagccatccg
      421 tgttgttcaa gccacagtcg gcgatttcgg gcagtaagct caggatgctt aataaccgtt
      481 tctatcccgt tccccgttaa ttcaattacc gggaattctg taatttggaa ccctcgccga
      541 ttcatcctca ggatgtggcc actggtgtcc aggataatga caacatcaaa aacgttatca
      601 agaatgagct ggttttcgcg gtttgtcaga ttccggaata aaccaaggtg gtttaccgta
      661 taaaaaaccg taccatacca gagcaccagg acccaaaaaa aaccggtaag ggcatggaaa
      721 aaacaaataa gaagaccgag cccgtaggca acaaaaacga gcgcgatcca ttcaccgtcc
      781 ctgcgggcac gatcgtcggt agcgctacga atccaggttg aaagaaagga ccatgaatag
      841 ccaaagacca gggatgtggc aacggcaatt atggcataca cccctcccgg ccggggaagc
      901 atcaaagata ttaccgacaa aggaatagtc gaaagatacg agataaaaaa atagcgggat
      961 cgccgtatag aggtcatgtc caccaaaaag gacaggtaca aaattccgga cacccaacag
     1021 gaaagcacaa aggcaaattg aatcgagatt accgacgcaa gtattgaaag atgcatcagg
     1081 gaaaagattg aaatagaagt aaaaaacgac aggccaaggg caaacaggcg ccactgcagc
     1141 gatgttttat ggacaatcct catattggcc agggtaaacc ccgccaacac ggcaagcgcg
     1201 aatgtggtat tcagtataaa cgtcaggtcc atctccactt ccttccgatt gacgggaggg
     1261 aaagcatcaa gtatccttcc atcatgaaaa tctggattaa ataccttgcc ggtgtcctta
     1321 tcggcctcgc cttttccctg gtagcgcccc accaaaaccc gattttttcc gacataacgg
     1381 cctacctgtc ggccctcgtc gttcaattcg gacgctattc cctgtacccg gtgctttttt
     1441 tcagctttac catcggggtc tttgaactgc gggaaagtaa aagccttatg aaaatcggca
     1501 gtatcgcggc tgcggttatc attatagcaa ctattttagc agccgtcacg ggacttcttt
     1561 ccgtccttat ccgtaacccg tcccgaatac ccatattcgt agagggcgcc tcggaaatag
     1621 gtgatatcgg cataaaagaa tctttgatgc aactctttcc ttccagcgga ttcgaggtct
     1681 ttaccaacgg cctgtttatc cttcccctgt gtatctttgg cggttttgcc ggtgcgggtt
     1741 gcgcggttga taaaacggct tccaaggcag ccttaaccct ctttgattcg ctttcccgcg
     1801 tgttttacgc cattatgtct ttctttgtgg acatcctttc aatcgggatg atcgccctgt
     1861 ccgtacactg gctgcttgag ttccgcctta tgatatccag caaggtattc accgatttta
     1921 ttcttttact tgcctttaat tttttcttcc ttgccctggt tctttaccca gccctgattc
     1981 gctttgtatg cggaaaggta aacccctacc gggttctcta tgcaggcata gcctcgatct
     2041 tggccgcctt cttctcggga gactccaaca tgacgctccc tattttactc aggcacgcaa
     2101 acgaaagcct tggggtgcgc cgtcgggttt cttcggttac catgcccatg ttttccatat
     2161 tcagccggcc aggcacggcc atggcggtta ccgtgagctt tatcgtcatt ctaaaatcct
     2221 attcaagcct tggcattgtc tttaccgaca tgctctggct tcttggcgta gcgattcttt
     2281 tgtctttctt tttgggaaga ttcccctcgg gaggggcata catcgccctg gcgacggtat
     2341 gtatgctcta tggacgcgga tttgaagctg gctacctgat tcttaaaccg gcagcctttt
     2401 ttatctgttc cattgccgcg gccaccgacg cactaacatc cctgatggga acgtacgcca
     2461 tggccaagcg atgggatatg gtgcatcacc gggaacttag attctttatc taactgtgtt
     2521 atacgtgatg aatcacgtcg cgcggttttg atccgttagc cggaccgaca atgccgcgct
     2581 gttccatctc ttccacgagc cgggcagcgc ggttataacc gatcttcagt ttgcgttgaa
     2641 tgtaggaagc ggacgcctta cccgcaagca tgacaatctc cagagcctta tcatacaagg
     2701 ggtcatcccc gtcggagaac aaggatgcat cgataggact gtcatcctca tcatcgacaa
     2761 agatttcttc gtcaatatat tcaggctctc ccaaggtttt aacgtgttca acaacccgct
     2821 caacctcttc ctctgacaca taggcgcctt gaatacgcac ggggaaagga tcggtcgcgc
     2881 tcgcatagag catatccccc cgacccaaaa gcttttccgc acccacctga tcgataataa
     2941 tacggctgtc cattttggag gcaaccataa acgcaattcg tgtgggaata tttgccttga
     3001 ttaaaccggt aataacatcg atggacggcc tctgcgtggc aagaaccaga tgaataccaa
     3061 cagcacggct cattgcgcaa agacgggcca cggtagattc tagctcctta ccggttgtag
     3121 ccattaaatc cgcaaactca tcgataataa ctaccagata cggaatcttc tcggtagcta
     3181 tctttcgttc tttaatacga tggttatagc tgcggatatc tcgcacaccc ataccatcaa
     3241 gcagggcata ccgcctttcc atctcgcaca agcaatactg cagtgattga aaggcttttt
     3301 tgggctcggt aataacggga gccaacaaat ggcctatatc gttgtaaagc ttaagctcta
     3361 caattttggg atctatcaaa ataagcttaa cgtcttccgg agagcgcttg tacaaaatgg
     3421 acaagataat ggagttgacg cataccgatt tacccgaacc ggtagaacct gcaatcaaca
     3481 gatggggtgt tgcggcaaga tcaaggagct gagcctcgcc ggtaatatct ttgcctaaca
     3541 caacaggaat ttccatcttc tttgcttgtg gggaatcgtt ttcgataatc tcgcggaag
//
LOCUS       DAOQXQ010000091         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_27062_length_3582_cov_7.982705, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000091 DAOQXQ010000000
VERSION     DAOQXQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27062_length_3582_cov_7.982705"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKH81_02270"
     CDS             <1..243
                     /locus_tag="PKH81_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96893.1"
                     /translation="AKLKRQFVVKLQDSSKGGNAWQTQTEIVKADLETDKWIELTFDF
                     SGVSTRQDYDKIVIQFGAEGHAGPGIFFFDDFRFKK"
     gene            373..1317
                     /locus_tag="PKH81_02275"
     CDS             373..1317
                     /locus_tag="PKH81_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNQ96894.1"
                     /translation="MPDTNKWWYETGGSGWGNNELQYYVAGVAREDTLALISDGTLKI
                     RALKKRYFSMDYVSIRMNTKENWKYGYFEMKAKVPGKRGSWAAFWMMPQNFTAWPLDG
                     EIDIMEYVGYRPNVTQSSVHTQSYNHVAHTEKTATKNIANAETEFHVYGLEWTEDKIT
                     GYVDGIPYFTFANDKLGDKNTWPFDAPFYLKLNLAIGGNWGGLMGIDENVCPATYEID
                     YVRVYQSVKTDRPALDAQADSPFTITPTLARDRVVVSSDNSRKYTVSVTDLQGRLIEK
                     LTNCMEDTIIDCSGWAKGLYVFTATNGVNSHSDKVIKN"
     gene            1334..>3582
                     /gene="bglX"
                     /locus_tag="PKH81_02280"
     CDS             1334..>3582
                     /gene="bglX"
                     /locus_tag="PKH81_02280"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNQ96895.1"
                     /translation="MKRASMKLNVLIAISSAILVSCQSSTSVKSNDVEKRVEALLSQM
                     TLEEKIGQMNQISPWDDPKAIAELIKKGEVGSILNVADPQTINAYQRTAVEQSRLGIP
                     LIVGRDVIHGFKTIFPIPLGQAASFNPDLIEKGARIAAIEASSVGVRWTFAPMVDISR
                     DPRWGRIAESLGEDTYLTSVLGAAMVKGFQGDSLNNPTSIAACPKHFVGYGAAEGGRD
                     YNSTHIPERLLRNVYLPSFEAAAKAGAATYMTSFNDNDGIPASGNGYILKDVLRKEWG
                     FDGFVVSDWASVGEMIAHGFCADGKEAALKAVNAGVDMEMVSYHYVNHLKELISEGKV
                     KEETIDNAVRNILRVKFRLGLFENPYVDESKGDVLYAASHLEAAKEAAIESAVLLKNE
                     GSVLPLGESVKTIAVIGPMADAPHDQMGTWVFDGDKNYTQTPLKALESAYGDKIKIVY
                     APGVSYSRDTNMSGIAAAVRAASSADVVLAFVGEESILSGEAHCLANLNLQGAQSALI
                     EAVANTGKPLVTIVMAGRPLTIGKEAALSDALLYSFHPGTMGGPALADLLFGKSVPSG
                     KLPVTFPKEVGQIPLYYSHNSTGRPFQGTETMLQDIPAEAGQTSLGNTSFYLDAGNDP
                     LYPFGYGLSYSTFKYDNLKLSSEKLKEGDVLTVSFDLTNTGKYDATEVAQLYVQDRFG
                     SVTRPVKELKRFERIALKSGESRSVSFSLPIEELAFWNIDMKKVVEAGDFNLWVGGDS
                     RAALKGSFVV"
BASE COUNT          892 a          847 c         1040 g          803 t
ORIGIN      
        1 gctaagctga aacgtcagtt tgtagttaaa ctacaggatt caagcaaggg cggtaacgca
       61 tggcagacgc agaccgaaat tgtgaaagcc gatctggaaa ccgacaaatg gattgaactt
      121 accttcgatt tcagcggcgt aagcacccgc caggattacg ataagattgt aattcagttc
      181 ggagccgaag gacatgcagg tccgggtatt ttcttctttg atgactttcg ttttaaaaag
      241 taaataatgt gttatagaag tgttttagta cagattatcc ttctggggtg ctgctgcctg
      301 cttggtcggg cgcaggcccc cgagggctac tcgttggttt ggtccgacga gttttcggct
      361 tccacacctt cgttgccaga caccaacaag tggtggtacg aaaccggcgg aagcggatgg
      421 ggtaacaatg agctgcagta ttatgtggcc ggtgtggcac gggaagacac gttggccctt
      481 atttcggatg gaacgctgaa gatcagagcg ctcaagaagc gttatttcag tatggattac
      541 gtttccatcc ggatgaatac aaaagaaaac tggaagtacg gatacttcga aatgaaagcc
      601 aaagtgccgg gcaagcgggg ttcgtgggct gccttctgga tgatgcctca gaactttacc
      661 gcatggccgt tggacggaga gatcgatatc atggaatatg tgggatacag gcctaatgtc
      721 acccagtcgt ccgtacatac ccaaagttac aatcacgtgg cgcatacgga aaaaacggcg
      781 actaaaaaca ttgccaatgc cgaaacggaa tttcacgtgt acggactgga gtggacggaa
      841 gataagataa caggctatgt agatgggatt ccctatttca cctttgccaa cgataagttg
      901 ggagataaaa acacctggcc tttcgatgct cctttttacc tgaagctgaa tctggctatc
      961 ggaggaaact gggggggact gatgggcata gacgagaatg tttgtcccgc tacctacgaa
     1021 attgattacg tacgtgtcta tcaatcggtc aaaaccgacc gtcctgctct tgacgcgcag
     1081 gccgattcgc ccttcaccat tacgcctacg ctggcacggg acagggtagt ggtgtcgtcg
     1141 gacaattcga ggaaatatac tgtaagcgta accgatttac agggcagact cattgaaaag
     1201 ctgaccaatt gcatggaaga taccatcatt gattgttccg gatgggccaa aggattgtat
     1261 gtgtttacag caaccaacgg tgtaaacagt cattcggaca aagtaataaa gaattaaaat
     1321 aaagaatgta cagatgaaac gagcaagtat gaaattgaac gtactgattg ccatatcatc
     1381 ggccatattg gtttcatgcc aaagctccac ttcggtaaaa agcaacgatg tggagaagag
     1441 ggtggaggcc ttgctttccc agatgacgct ggaagagaag atcggccaga tgaatcagat
     1501 cagtccctgg gatgatccta aagcaatcgc cgagctgatc aagaagggtg aagtgggttc
     1561 gatccttaat gtggccgatc cgcagaccat caatgcctac caacgtacgg cagtggagca
     1621 gtcgcgcctg ggcattccgc tgattgtggg ccgtgatgtg atccatggat tcaagacgat
     1681 tttccctatt ccgctgggac aggcggcttc gttcaatccg gatctgatcg agaagggagc
     1741 gcgcatcgcc gccatcgaag cctcttcggt aggggtgcgg tggactttcg caccgatggt
     1801 ggatatctcc cgcgaccctc gctggggccg cattgcggag agtctgggtg aagacaccta
     1861 cctgacctcg gtattgggcg ccgccatggt gaagggattc cagggagatt cgctgaataa
     1921 tcccacctcc atcgcagctt gtccgaagca ctttgtgggc tacggggcag cagaaggtgg
     1981 ccgcgactat aactccaccc atattccgga acggttgctt cgcaatgtgt acctgccctc
     2041 tttcgaggcg gcagccaaag ccggagcagc tacctatatg acctccttta acgacaacga
     2101 cggcatccct gcctcgggca acggttatat cctgaaggat gtactgcgca aggagtgggg
     2161 cttcgacggc tttgtggtat cggactgggc ttcggtaggg gagatgatcg cccacggctt
     2221 ctgtgccgac gggaaggaag cggcgctgaa ggctgtgaat gcgggcgtcg acatggagat
     2281 ggtaagctac cattacgtga accacctgaa ggaactgatc tcggaaggta aggtgaagga
     2341 ggagacgatc gacaatgcag tacgtaacat cctgcgcgtg aagttccgtc tcgggttgtt
     2401 tgagaatccg tatgtggatg agagcaaggg agatgttctt tacgcagctt cacacctgga
     2461 agccgcgaag gaggccgcca tcgagtcggc cgtacttctg aagaacgaag gttcggtatt
     2521 acctctggga gaatccgtaa agaccatcgc cgtaatcggt ccgatggcgg atgcaccgca
     2581 cgaccagatg ggtacgtggg tgttcgacgg ggacaagaac tatacccaaa cacccctgaa
     2641 ggcattggaa tcagcctatg gcgataagat caagatcgtg tatgccccgg gcgtaagcta
     2701 cagccgggat acgaacatga gtggtatcgc cgcagcggta cgtgccgcat catcggcaga
     2761 tgtggtgttg gcttttgtag gagaggaatc catcctgtcc ggcgaggccc actgtctggc
     2821 caacctgaac cttcagggcg cgcagagcgc tttgatcgag gcggtagcca atacgggcaa
     2881 acctctggtt acgatcgtaa tggcgggtag accgctaact atcggcaaag aggcagcctt
     2941 gtccgatgcc ttgctttaca gcttccatcc gggcacgatg ggcggcccgg ccctggcgga
     3001 cctgctcttc ggcaagtcag tgcccagcgg caagctgcca gtgaccttcc cgaaggaggt
     3061 aggtcagatt cccttgtatt acagtcacaa cagcacgggc cgtcctttcc agggtacgga
     3121 gacgatgctg caggatatcc ctgcggaagc tggacagacc tcgctgggta acacctcgtt
     3181 ctatctggat gcgggtaacg atccgctgta tccctttggt tacggcttgt cttacagcac
     3241 attcaaatac gacaacctga agctatcttc agagaaactg aaggagggcg atgtacttac
     3301 ggtaagcttc gatctgacca atacgggcaa atacgatgca acggaggtag cgcagctgta
     3361 tgttcaggac cgtttcggtt cagtaacgcg tccggtaaag gagctgaagc gctttgaacg
     3421 catcgctttg aagtcgggcg aaagccgcag tgtaagcttc agtctgccga tagaggagct
     3481 cgccttctgg aacatcgata tgaagaaggt tgttgaggcc ggtgatttca acctgtgggt
     3541 aggaggcgac agccgtgcgg cattgaaagg ttctttcgtt gt
//
LOCUS       DAOQXQ010000092         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_27370_length_3555_cov_4.125714, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000092 DAOQXQ010000000
VERSION     DAOQXQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27370_length_3555_cov_4.125714"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3555)
                     /locus_tag="PKH81_02285"
     CDS             complement(<1..>3555)
                     /locus_tag="PKH81_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96896.1"
                     /translation="LEFTITDTTGNVTVDSRTVWADQATDIPAITLNNLDQSKTAPAD
                     AGSNLLSGSTISFNVTDDDGVAPSGIAIVVLDSTDTPVTLAAPTISGIAAARVVSQTL
                     SGLPDGEYSLQITATDNALAISATMPRVYFSYDNNPPTLSAPAIPQYTRTLFGMTGGT
                     AADGTALDPATPIVITQSRDGSSIVLTGVILSDVTTKSVSWAIQNLPRAADNTPAPLS
                     AATNGLYEYTITAQDRAGRTVQTTRSIRFDVSAPSVTMATPQAPSADVPDYGNILGTT
                     ALISGTSDDSATGASGVKAVYYNFDGSATRITAGGTVAWSSTATLVAPEGPKTLYVWA
                     EDNAGNISAAPSTRTFVYDTAIPVASITAIPLSQSALFSLDGLATDGYGVSSVSITQN
                     GVALPGLTTGGTTQSRTFAVANLPRNPASPADPLLETGTYVYRVVATDATGKTSTPAE
                     ITIKVDITPPNVNEITAPLASPPESMSIGVNAISGTAYIFRGNASDADSPISRVWYVI
                     NGSATAPVVSTTPTDAGYTAFTTAGTWALTKNIPAELPEGLNRYLHVLAEDSAGNRSV
                     SATTVRFDVDRADPVLSDILLDEVAFNTTSIENKLAQYTLSGLASDTHGIASVAITQN
                     GAAIPPAALTLEGTAQATTWSLANLPYGTTDNDTYEYVITATDLTGKTHSLTRRVRYD
                     TVGPELTVMSPAPGSWLNALSVSVNGIATDSTAVSAVYYQATAHDEATAPTTPADPSV
                     AANWTSAGWTGATGTNSWNLSVTEASQGRKKLWFSAVDALGNVSATTTALNYGVDTET
                     PNSAIVSAVPANTRALFSMNGTATDSNALADLASDRVTITQSKDGGAALTIPSASITI
                     GGTLTNGTWSLANLPRDPGNMNNPVALSAASDGEYEYVLTVTDVAGKIRQRTAVTRFD
                     LTSGTAIIASPSAPTGSVPDAPNTVGANFTFSGTASDAGSGVKTVYYNLVDDTGTWIT
                     AAGMTAWSASGAMSGTEGLRTLYVWVEDNAENVSTAFTRSFIYDTAPPVATITPMSTK
                     VNADFQIAGTALDNYGVDIASITVTQNGVAINTNGVVHVSGSDTNRTWALQNLPRNPA
                     SIDDLLLVDGNYSYVVTVTDKAGKQTILDPITITVDQTPPNVNTITSPGAGQTGVNAL
                     SGTSYVFKGSATDAGVGVAKIWYAIDTNAAAPAITTTPV"
BASE COUNT          830 a          883 c          998 g          844 t
ORIGIN      
        1 cacaggggtt gtggtaatag cgggtgcggc ggcatttgtg tcgatggcgt accagatttt
       61 tgcaacgcca acaccggcat cagtagcaga acccttaaac acatatgagg ttcccgaaag
      121 ggcgtttaca ccggtttgac ctgctcccgg ggatgtaatc gtatttacgt tgggaggagt
      181 ctgatcaaca gtaatcgtaa taggatcaag aatggtttgc ttaccagcct tgtcggttac
      241 ggtaactaca taagaatagt taccatctac taaaagaaga tcgtctatag acgcaggatt
      301 tctgggaagg ttttgcagag cccacgttct gttggtatcg ctacccgata catggaccac
      361 accattggtg tttatcgcca ctccattttg cgtaacggtt attgatgcga tatcaactcc
      421 atagttatca agggcagttc cggcaatctg gaaatctgcg tttaccttgg tggacatggg
      481 agtaattgtc gctaccggag gagcagtgtc gtagataaag gagcgagtaa aggcagtgga
      541 cacattctcc gcgttatcct ctacccatac atacaaggtc cgcaatccct ctgttccact
      601 catcgcgccg gaggcagacc aggcagtcat accggctgcg gtaatccagg taccggtatc
      661 gtcgacaaga ttataataca cggttttaac gccgcttcct gcgtcggaag cggtaccgct
      721 aaaggtaaag ttcgctccaa cggtattagg cgcatcagga acagaaccag ttggtgcaga
      781 agggctggcg atgatggcag tgccgctcgt caagtcaaag cgggttaccg ccgttctctg
      841 cctgatcttt ccggcaacgt ctgttaccgt tagtacatat tcatattcac catcggaggc
      901 agcagaaaga gccaccggat tattcatgtt acccgggtct cgcggtaaat tcgcgagcga
      961 ccaggtgccg ttggtaaggg ttcctccaat ggttattgag gcagaaggaa tggtaagggc
     1021 tgctccaccg tccttacttt gcgtaatggt aacacgatcg gatgcaaggt ccgccagtgc
     1081 gtttgaatca gttgcggttc cattcatgct aaacaaggcg cgagtattcg cgggaacagc
     1141 ggagacaatc gccgagtttg gagtctcggt gtctactcca taattaagag cggttgtggt
     1201 agccgatacg ttacccaacg catccacagc cgagaaccac agtttcttcc tgccctgaga
     1261 ggcttccgtt actgataagt tccagctgtt agtccctgtg gctcctgtcc aaccggcgct
     1321 tgtccagttg gcggcaacag agggatcggc cggcgtcgtc ggggctgtcg cctcatcatg
     1381 cgcagttgcc tgataataca ctgccgaaac agcggtagag tcagtggcaa tgccgttaac
     1441 cgaaacagac aaggcgttga gccatgaacc tggagcaggc gacataaccg taagctctgg
     1501 tcccacggtg tcgtagcgga cacggcgggt aagtgaatgc gttttccccg taagatcggt
     1561 agcggtaata acgtattcat aggtgtcgtt atccgttgta ccatacggca ggttagctaa
     1621 ggaccaagtc gtcgcctgcg cggttccttc aagagttaga gcggccggag gtattgccgc
     1681 tccgttttgc gtaatcgcga cggaggctat tccatgagtg tcggaggcaa gtccggaaag
     1741 ggtatattgc gccagtttgt tttctatcga agtggtatta aaggccactt catccagaag
     1801 gatgtccgac agaacagggt ctgcgcggtc tacgtcaaag cgcacggttg tcgcagagac
     1861 agaacggttc cccgctgaat cctcggcaag aacatggaga tagcgattca atccctcggg
     1921 tagttctgca ggaatatttt tggtaagcgc ccaggtgcca gccgtggtaa acgcggtata
     1981 gcctgcgtcg gttggcgtag tgcttactac gggcgctgtt gcgctgccgt tgataacata
     2041 ccacacacgg gaaataggac tgtccgcatc gctggcgtta ccacggaaga tataggcagt
     2101 accgctaata gcgttaacgc ctatggacat gctttccggc ggagatgcca gaggagcagt
     2161 tatctcgtta acattgggag gcgtaatatc tacctttata gtaatttcag ccggtgtgct
     2221 ggtcttaccc gtagcgtctg tcgcgacaac acgatacaca taggttcccg tctctaacaa
     2281 gggatcggcc ggacttgcgg gatttcgcgg aaggttggca acagcaaagg ttctcgattg
     2341 ggtagtgccg ccagttgtaa gaccgggaag ggctacgccg ttttgcgtaa ttgataccga
     2401 agacactcca tagccgtcgg tcgcaagccc gtcaagcgag aacaaagcgc tctggctcag
     2461 cggaatcgcg gtgatagacg caacaggaat tgccgtgtca tatacaaagg ttctggtaga
     2521 aggagcagcg gatatatttc cggcattgtc ttctgcccac acatacaagg ttttgggtcc
     2581 ttctggagcg acaagggttg ccgttgaact ccatgcaact gttccgcccg cagtaatccg
     2641 tgtggcgctt ccgtcaaagt tataatacac agcctttacg ccgctcgctc cagtcgcact
     2701 gtcgtcagag gtaccggata taagagccgt agtgcccaga atattcccat aatcaggtac
     2761 atcggccgat ggagcctgag gcgtcgccat ggtaacagac ggcgcactta catcaaaccg
     2821 tatagaccga gtagtctgca cggttcgtcc agcacggtcc tgcgcagtga tggtgtattc
     2881 gtataaacca ttagttgcgg cagagagcgg cgcgggggta ttgtcggccg ctcgtggcaa
     2941 gttctggatt gcccagctaa cagacttagt cgttacatcg gagaggataa ctccggtaag
     3001 aacaattgaa ctgccgtccc tgctttgtgt aatcacaatc ggcgtagcgg gatcaagcgc
     3061 tgttccatct gctgcggttc ctcccgtcat accaaaaaga gtacgggtat actggggaat
     3121 agccggagcg ctcagggtag gtggattatt atcgtagcta aagtacaccc tgggcatcgt
     3181 tgccgatatc gcaagggcat tatctgtcgc ggtaatctgc aaggaatact cgccatccgg
     3241 aaggcctgaa agggtttggc ttaccacgcg tgcggccgca attcccgata tagtcggggc
     3301 ggcaagggta accggtgtgt cggtactatc tagaaccact atcgcgatac ccgatggggc
     3361 cacgccatcg tcgtcggtaa cgttaaagct gatagtgcta ccactcagca ggttggatcc
     3421 cgcatccgct ggagcggtct tgctttgatc aaggttattc aaggtgattg ccgggatatc
     3481 cgttgcctga tcggcccaga cagtcctgct gtcgacggtt acgttacctg tcgtatcggt
     3541 aatcgtaaac tcaag
//
LOCUS       DAOQXQ010000093         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_27712_length_3522_cov_3.113354, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000093 DAOQXQ010000000
VERSION     DAOQXQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27712_length_3522_cov_3.113354"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKH81_02290"
     CDS             <1..1317
                     /locus_tag="PKH81_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNQ96897.1"
                     /translation="VADSIEKVFSERLSDFYGMLASHYSQAEDLDKTEEYLIKAGEES
                     LKSSASAEALHYYQQALDLYLRKYGDKADPKKVAMLEKNIALSLYNRGQYTECVPYFD
                     KVLAFYDEIIPRHKIARVLKLGVGILSSVSALYLPYLHWKKDPQGIPMGGYGLSLTSR
                     DMAKLGFLYLNKGLWDGKQIIPRSWVEEAVKAHTATPGSFSPGYFYGYQWWISPDKKT
                     YIAQGYGGQNIFVNPDLDLIIVTTAALNYRRLNDYYTLITQEIPASCVSAGKIPENKE
                     GEEALDSLLAAWASPPPAQAIRLPSAFAQNTDTTFVFDTNEQDLQSLTITLDSEKGII
                     IRQTYQSKRGEQGITECIGSLGGEYRLSRSVMNEARYQFGDTPIQTACRLIESGDEQT
                     LLMEIVPLGIVEGPFLDRFSVENQEITFTRVNQANRRSVTIKGRKT"
     gene            1372..>1634
                     /locus_tag="PKH81_02295"
     CDS             1372..>1634
                     /locus_tag="PKH81_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HNQ96898.1"
                     /translation="MAREKKSEGVIHTSSSRLGMDAPSFKDSYFRHLEYSLGKDVYSA
                     TDHDRYSACALAARDRLIERWIATQQTYYNKNAKRVYYLSLEFL"
     assembly_gap    1635..1644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1645..>3522
                     /locus_tag="PKH81_02300"
     CDS             <1645..>3522
                     /locus_tag="PKH81_02300"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNQ96899.1"
                     /translation="IGRTLGNSLINLDLYEPAARALREIGLSLEDLREKEWDAGLGNG
                     GLGRLAACFMDSLATLELPAMGYGIRYDYGIFFQSIKNGYQIETPDNWLRFGNVWEFP
                     RPEYSYLVNFYGKAEGYVDATGSQRRRWADVEQIVAVGYDTPIPGYRNNTVNNLRLWS
                     AKSSREFDLGYFNGGDYIKAVADKSISENITRVLYPNDAVHEGKELRLKQEYFFVSAT
                     IQDIIRRFRKTHGNFKDFPQQVAIQLNDTHPAIAIAELMRILVDSECLDWETAWDITV
                     HTFGYTNHTILPEALERWPVDLIGKVLPRHLEIIYEINRRFMNDIAASFPGDTALLQS
                     CSLIEEGTEKKVRMANLAIAGSHSINGVAALHSEILKTRLFKDFYQLWPERFNNKTNG
                     ITQRRWLKLCNSELASLIDETIGERWTKDLSALRDLEPFANDASFREKWRAVKMAKKK
                     SLHAYIMGHNGVDTDVHSLFDIQVKRLHEYKRQLLNVLHVITLYNRIKDNPSADITNR
                     TIIFAGKAAPGYWRAKLIIKLINSVAHTIANDKEVSKRLKVLFLRNYAVSNAEKIIPA
                     ADLSEQISTAGTEASGTGNMKFALNGALTIGTLDGANIEIMEEVGKENIFIFGLNAEE
                     VD"
BASE COUNT         1011 a          812 c          816 g          873 t
ORIGIN      
        1 gtggcggatt ccatagagaa ggtcttcagt gagaggctga gtgattttta cggcatgctc
       61 gcttctcact acagccaggc agaggacctg gataaaacgg aagagtatct aatcaaggca
      121 ggggaggaat cactgaagtc atcagcttct gctgaagcgt tgcattacta ccaacaggcc
      181 ctggatcttt acctcagaaa atatggtgac aaagcagatc cgaaaaaagt ggccatgctt
      241 gagaagaaca ttgccctgtc tttatataat cgaggacagt acactgagtg tgtaccctat
      301 ttcgataagg tgcttgcttt ctatgatgaa ataataccac ggcataagat cgccagggta
      361 ttgaaattgg gtgttggtat cttgtcttca gtttctgcac tttatcttcc atacctccac
      421 tggaaaaaag atccgcaagg aattcccatg ggtggttatg gactgtccct taccagccgt
      481 gatatggcaa aacttggatt cttgtacctc aataaaggtc tttgggatgg gaaacagatt
      541 attccccgtt catgggtcga agaggctgtt aaagcgcaca ccgcaacgcc aggttccttt
      601 tcccccggtt atttttatgg atatcaatgg tggatatctc ccgacaaaaa aacatatatc
      661 gcacaggggt atggtgggca aaacatcttt gtcaatcctg acctggatct gattattgta
      721 acaaccgcag cccttaatta tcgcaggctt aatgattatt acacccttat tacccaggag
      781 atccctgctt cctgtgtgtc ggcgggaaag attccggaaa acaaagaagg ggaagaagcc
      841 ttggactcac tgttggcagc ctgggcctcg ccccctccag ctcaggccat tcgtcttcct
      901 tccgcctttg cacaaaacac cgatactacc tttgtgtttg acacaaacga acaagacctg
      961 caaagcctta ccattaccct tgatagcgaa aaaggaatta tcattcggca gacctaccaa
     1021 tccaaaaggg gcgaacaggg gattaccgag tgcatcggaa gccttggcgg tgaatacagg
     1081 ttatctcgtt cagtgatgaa cgaggctcga taccaattcg gggacacccc cattcaaacg
     1141 gcttgccgct taattgaatc aggagatgag caaaccctgc ttatggaaat agttccgctt
     1201 ggcatagttg aagggccctt tcttgatcgc ttctcagtcg agaatcaaga aataacgttt
     1261 acccgtgtaa atcaggccaa ccggcgctct gttaccataa aaggtcgcaa aacataactg
     1321 tcgtagacta tacttctagg tgttatacca acacactaaa gaggcatatc tatggcaaga
     1381 gaaaaaaaaa gtgaaggcgt tatccatacc agctcttcca ggcttggcat ggatgcgccg
     1441 tcttttaagg attcttactt tcgtcatctg gaatactcct tgggtaagga tgtatactcg
     1501 gccactgatc atgaccgcta tagcgcatgt gctctagccg cacgggatcg ccttattgag
     1561 cgatggatcg cgacacaaca aacgtattac aataaaaacg ccaaacgggt atattacctc
     1621 tcccttgaat ttctnnnnnn nnnnattggc agaacacttg gtaacagcct tatcaatctg
     1681 gacttatatg agccagccgc tcgggccttg cgggagattg gcctatccct tgaggacttg
     1741 cgagaaaaag aatgggacgc gggccttggt aacgggggat taggcagact tgcagcctgt
     1801 tttatggatt cacttgccac actggaattg ccggcaatgg gatacggtat ccgctatgat
     1861 tacgggattt tttttcaaag cattaaaaat gggtatcaga tagaaacacc ggataactgg
     1921 ctccgctttg gaaatgtttg ggagtttccc cgaccggaat attcatacct ggtcaatttt
     1981 tacggcaagg ccgagggata cgttgatgcc actggttccc agaggcggcg atgggcagat
     2041 gtagaacaga tcgtcgcagt cggctatgac acccctattc caggctaccg taataatacc
     2101 gtaaacaacc ttagactctg gtccgcaaaa agctcaaggg aatttgatct tggttatttt
     2161 aatggtgggg actacatcaa ggcagtagcg gataagtcca tctcggaaaa cataacccgg
     2221 gtactgtatc ccaacgatgc cgtacacgaa ggaaaagagt tgcgcctaaa gcaggagtat
     2281 ttttttgtat cggcaaccat acaagacatt atccgccgat tcagaaaaac tcatggaaac
     2341 ttcaaagatt tcccgcagca agtagccatc caacttaacg acacccatcc tgccatcgcg
     2401 attgccgagc ttatgagaat ccttgttgac agcgaatgtc ttgactggga aacagcctgg
     2461 gatattaccg tacacacctt tgggtatacc aatcacacta tccttccgga agcccttgag
     2521 cgatggccgg tagaccttat tggtaaggtc ctgccccgac atctggaaat aatctacgaa
     2581 ataaatcgcc gtttcatgaa tgacatagca gcttccttcc ccggagacac ggctttactt
     2641 caatcgtgtt cacttatcga ggaaggaact gaaaaaaaag tgagaatggc aaatcttgcc
     2701 atagcgggaa gccattcgat taacggagtc gctgcactgc atagcgaaat tctcaagacc
     2761 cgcttgttca aggatttcta tcaactatgg cccgaaagat ttaacaataa aaccaatggc
     2821 ataacgcaaa gaagatggct caagctctgc aatagtgagc tggcatcgct tatcgacgag
     2881 acaataggag agcgatggac aaaagattta tctgccttac gcgatcttga accatttgcc
     2941 aatgatgcaa gcttccgcga aaaatggcgg gcggttaaaa tggctaaaaa gaaatcgctt
     3001 cacgcgtata taatgggaca caatggggtg gatacggacg tgcactccct ttttgacatt
     3061 caggtaaaac gcctccatga gtataaacgt caactgctta atgttctgca cgttattacc
     3121 ttgtataacc gcataaagga taatccttcg gcagatatta caaatcgtac catcattttt
     3181 gccggtaaag cggctcccgg ttattggcga gctaaactga ttataaaact gattaactcg
     3241 gtggcgcaca cgatagcgaa tgacaaagag gtatccaaac gattaaaggt tctcttcctt
     3301 cgaaattacg cagtctcgaa cgcggaaaaa atcatcccgg ctgctgacct ttccgagcaa
     3361 atttccacag cgggtacgga agcatccgga acaggtaaca tgaagttcgc cttaaacggt
     3421 gcgctcacca ttggcaccct tgatggggca aatatcgaaa taatggaaga agtgggtaaa
     3481 gagaacatct ttatttttgg cctcaatgca gaagaagtag ac
//
LOCUS       DAOQXQ010000094         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_27747_length_3519_cov_3.756351, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000094 DAOQXQ010000000
VERSION     DAOQXQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27747_length_3519_cov_3.756351"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKH81_02305"
     CDS             <1..714
                     /locus_tag="PKH81_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ96900.1"
                     /translation="DILRIIEYINKEHSQVIMFTSGYLLEQYAADLKKAGLTSVIVSL
                     NATNAAEHDAIKKKPGTFDSAIRGIKAARKAGLLVGIASVVYKKHLGTDHIQNIIEMG
                     KSLKANQIIFFDSIPAGAHLEDTEVIWNDQDVDRLIEICHEYHSKPNYPGVLAYSYIN
                     SYRSIGCYAGTIYFYVSPYGDVCPCDFCSFSVGNVLEEPVHVLWDRFSDHKEFQRSSL
                     SGCKMKDSAFRKKFAGVQE"
     gene            721..2028
                     /locus_tag="PKH81_02310"
     CDS             721..2028
                     /locus_tag="PKH81_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ96901.1"
                     /translation="MKVIHSTRSYCCDCHCYHDALYYIDSGCVFFKMLCDTPRTTLIS
                     RHPDIYMHLWNKSYFTTKNVQTIPVSNRIEITNACNFTCNLCYAGASPNKNSFISLEK
                     IARISEYLRREKRTDIGLTGGEPTLHPELLKIVSIFKKAGHIISLYSNGYLIAKEPTL
                     AGKLKEQGVYKCFLQFDTLNPQTHQLLRNNSFVKEKLQALQNLTKAKIKVSPICVMVK
                     ENAHEVKELLDQVRAMGPYFGELIFLTAIQDTGRFTLEKGSPLLRDDIIVSIIKHSGI
                     PGIGIEHFWPFPKFLPLEVNLHPDCATIVYLLIRNGSIELLDSYINIKRLYSLCSKMK
                     KNTSYRVAYLKVLFLLLPCIKWTKCITVAKIIYSYITKKGGCFINYIVVESFMTRYCQ
                     DAERLHSCTSHHVLDENISVSACVFNQDGDIGSQCNRFKNPQI"
     gene            2055..2813
                     /locus_tag="PKH81_02315"
     CDS             2055..2813
                     /locus_tag="PKH81_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ96902.1"
                     /translation="MSVKDFYSDILGSAGLSFPVLWRVIRSAKLSRSIPLIFNFVVPG
                     RIINALYRQIHKNKFIPKSMVIEPSYRCNMSCADCYAPEETTLMSGELLDSIITQAKK
                     LGVFRFDLMGGEPTLPEVIDMALPVINKNKNCVFNICTNCTLIDQAFIDKVKGSKNIF
                     YLLSMEGNKETTEARRGKGSYDAYLRSVQLLRQNKMAFSLSMTLDPNSWKEQISEQVI
                     GDYVEAGGAIIYSYPRFNNQNKKFYELPRIEFLK"
     gene            2820..3134
                     /locus_tag="PKH81_02320"
     CDS             2820..3134
                     /locus_tag="PKH81_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNQ96903.1"
                     /translation="MLCKKYAVYWGDVHYGKMKAYKGIIPRHNNQVCINPSGKVRPYR
                     FFAMPEFGDVSTTPLAEILASKELIDFKRESRTKANELLKDEKEELARQGFKVYNYKW
                     RM"
     gene            complement(3135..>3519)
                     /locus_tag="PKH81_02325"
     CDS             complement(3135..>3519)
                     /locus_tag="PKH81_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96904.1"
                     /translation="LALIQKQLTNNIATFDACLKGFRYYWPMHDLQNTLPEVSTILDP
                     IQQAGEGWLIASEILGFSKAGVHTIVCLQPFGCLANQVSAKGIEAGLKKKDPRLNVVY
                     LDLDYNTCEANYFNRLQMVIDNTGV"
BASE COUNT         1036 a          689 c          801 g          993 t
ORIGIN      
        1 gatatactgc gaatcataga atacataaac aaagagcaca gtcaggttat catgtttacc
       61 agcggctatc tgttggagca atatgccgct gatctaaaaa aagcagggct gacatcggtt
      121 atcgtcagtt tgaatgcgac gaacgcagcg gagcatgatg caatcaagaa aaaaccggga
      181 acctttgata gcgcaattcg cggtattaaa gccgcaagaa aggctggtct tttggttgga
      241 atcgcttctg ttgtatataa aaaacacctg ggaacggatc atattcagaa tattatcgaa
      301 atgggaaaat cccttaaagc taatcagata atcttttttg attctatccc cgccggagcc
      361 cacctggaag acacagaagt tatttggaac gatcaagatg tagatcggtt aatagaaatt
      421 tgccatgagt atcacagcaa acccaattat cccggcgtgc tcgcctatag ttacataaac
      481 tcgtatagaa gtattggctg ttacgccggt accatttatt tttacgtcag tccctatggg
      541 gacgtgtgcc cttgcgattt ttgttcgttt tcggttggaa acgttttgga agagcctgtg
      601 cacgttttat gggatcgatt tagcgatcat aaagaattcc agagatcttc cctgtcgggc
      661 tgcaagatga aggacagcgc attcagaaaa aaatttgccg gtgtacagga gtaataaact
      721 atgaaggtaa tccacagcac ccgatcgtac tgttgtgatt gccactgtta tcatgacgcc
      781 ctctattaca tagattcagg ctgcgttttt ttcaagatgc tatgcgatac tcccaggaca
      841 acgctcattt cgcgtcatcc tgacatctac atgcatctgt ggaataaaag ttattttacc
      901 accaagaatg ttcaaacgat tcctgtttcc aaccgaattg aaattaccaa cgcttgtaat
      961 tttacgtgta atctgtgtta tgccggggca agcccaaata aaaacagttt tatctctttg
     1021 gaaaagattg ctagaatttc cgagtattta cggcgagaga aaagaacaga catcgggtta
     1081 actggcggcg agccgacttt gcatccagag cttttgaaaa ttgtttcaat ttttaaaaag
     1141 gccgggcata tcatttcctt gtattccaat ggctacttga ttgcaaaaga gccaacactt
     1201 gcggggaaac tgaaagagca gggtgtgtat aagtgcttcc tgcaattcga cacattgaat
     1261 ccacagacgc accaactatt gaggaacaat tcttttgtta aagaaaaact gcaagccttg
     1321 cagaatttga caaaggcgaa gataaaggtt tcgcctattt gcgtcatggt aaaagaaaac
     1381 gcgcatgagg ttaaagagct tttagatcag gttagggcga tgggtccgta ttttggagag
     1441 ctcatatttt taacagccat acaggacacc ggcagattta cgcttgaaaa gggatctcct
     1501 ttgttgcgtg atgatattat tgtttcgata ataaaacact ccggaattcc ggggattggg
     1561 attgaacact tttggccctt tcccaagttc ttgcccttgg aagttaactt gcatcctgac
     1621 tgcgctacca tcgtatactt gttgatccga aatggttcta ttgaactact tgattcctac
     1681 atcaatatta agcgtctgta ttctctgtgt agcaagatga aaaaaaacac ctcgtacagg
     1741 gtggcgtatc ttaaggtgtt gtttctttta cttccctgca ttaaatggac taagtgtatt
     1801 accgtggcaa aaattatata ttcatatatc acaaagaaag gaggttgttt tataaattac
     1861 atcgtagtcg aaagttttat gacccgttac tgccaagatg cagagcgtct ccattcgtgt
     1921 accagtcacc atgttctgga tgaaaacata tccgtatcag cgtgtgtttt taatcaggat
     1981 ggggatatag gctcgcaatg taatagattc aagaatccgc agatatagtg ttgcaatttt
     2041 tataggagta tttcatgagt gtaaaggatt tttatagcga cattttggga agtgccggct
     2101 tgagctttcc ggtcttgtgg cgcgtgatta gaagcgcaaa gttgagtaga tcaatcccgc
     2161 ttatctttaa ttttgtggtt ccgggaagaa tcattaatgc cttataccgg caaatacata
     2221 aaaacaagtt catacctaaa tcaatggtga ttgaaccgag ctaccgctgt aatatgtctt
     2281 gcgcggattg ttatgcgcct gaggaaacaa cacttatgtc tggcgagctt cttgattcaa
     2341 ttattaccca agcaaagaaa cttggcgttt ttcgatttga cttgatgggc ggagagccaa
     2401 ccttgccgga ggttatcgac atggcgctcc cggtaatcaa caagaacaaa aattgcgtat
     2461 tcaatatttg tactaactgc accttgattg atcaagcttt tatcgacaag gtgaaaggct
     2521 ccaagaatat tttttattta ctgagcatgg aagggaataa agaaaccaca gaggcccgac
     2581 ggggaaaggg cagctacgac gcgtatttac gcagcgtgca actcttgcga caaaacaaga
     2641 tggctttttc cctgtctatg acgctggatc cgaacagctg gaaggaacag attagcgagc
     2701 aggtaatcgg cgattatgtg gaagcaggcg gagctattat ttattcgtac ccccgcttta
     2761 ataaccagaa taaaaagttt tatgaattgc caagaattga attccttaag tagattcaaa
     2821 tgctctgtaa aaagtacgcg gtctattggg gcgacgtgca ttatggaaag atgaaggctt
     2881 ataagggaat aattcccaga cacaacaatc aagtgtgtat caatccttcc ggaaaggttc
     2941 ggccgtatcg attctttgca atgcccgagt ttggagatgt gtctaccact cccctggcag
     3001 aaatccttgc atcaaaagag ttaatcgatt ttaaaaggga aagccgaacc aaagcaaatg
     3061 agctgcttaa agatgaaaaa gaagaattgg caaggcaggg ttttaaggtc tacaactata
     3121 agtggcgaat gtaattatac gccggtgtta tcaattacca tctgaaggcg attaaagtaa
     3181 tttgcttcgc aggtattata atcaagatcc agatagacaa cgttcagtcg ggggtccttc
     3241 tttttcaagc ctgcctctat tcccttggct gacacctggt tcgccaggca gccaaagggt
     3301 tggaggcata caatagtgtg gactccagct ttactaaacc cgaggatctc ggaggcgata
     3361 agccatcctt cccccgcttg ttggatggga tcaagaatgg tagagacctc gggcagtgtg
     3421 ttttgtaaat catgcattgg ccagtaatag cgaaaccctt taaggcacgc atcaaaggtt
     3481 gctatattgt tggtaagttg cttttgaata agagcaagg
//
LOCUS       DAOQXQ010000095         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_27850_length_3510_cov_2.832417, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000095 DAOQXQ010000000
VERSION     DAOQXQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27850_length_3510_cov_2.832417"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKH81_02330"
     CDS             <1..695
                     /locus_tag="PKH81_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ96905.1"
                     /translation="SDKYWSPEIVGIFRFDYRRFDEDAILVSIDRLQRILGLTQSIQQ
                     LVIYTDSPDSLVSIEQNIKRKLGELTIVTSWTDNYWVAMFRSMSGMFLIVFAVFQIVA
                     SFLIINTMVMIIHERIKEIGMMGALGMNRTEIVMVFFLEALMLSVLGSLVGMIIGGLS
                     SWIASLYPLDMNTFTGGGMKDLPMAGTLYLAFSWITCIKGFLFGIGVSALCTLFPSMK
                     SAFIEPVEALRR"
     gene            728..1468
                     /locus_tag="PKH81_02335"
     CDS             728..1468
                     /locus_tag="PKH81_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ96906.1"
                     /translation="MKRLRNALSLGIMIASICLSAYAQTAQEILSLVDDNEIYSSISY
                     EAEMHISYQGKTFIKKIRGYGRENKDSFIEFINQEDVGTKYLKKDGRLYVYSPDTEQV
                     MLISGHMLKESMMGSDYSYEDTIENDRLSKRYSSVVSGEEILGDRACWILDLTALKST
                     ESYPTQRLWVSKDTHEVLRFDLFALSGVRVKEYSLLESRLIKNRYFPVKSEMRDLMRK
                     GSKTVFTMDKVELDQKLSDSLFSTRNLR"
     gene            1465..2655
                     /locus_tag="PKH81_02340"
     CDS             1465..2655
                     /locus_tag="PKH81_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96907.1"
                     /translation="MIKIPITHTAILVCMWILVIALSPIYSETVISGVAETKGLGRIS
                     DDSYYVQAEQFANIRMRSTIGEYGEFHSAVQLIVSSNTENEFCSQAELERLYLSLVIE
                     RFDIKLGLQRLPFGYGFAFRPLDMFTQPSVLYPEARPKGVLSSLVTVYPRDELSFSFF
                     CGQQRDDVIQDTRDPVFYGLALTQHHSVFSTQFLYALSVSRDETQPTTGSGGFSTKVD
                     SLIGIILEALYTHDYSSPFEEGSVDHNIQVTAGFDYSWMSGKLYLVTQYCYSSAGYLD
                     SSDSISSLYTDPLWADLPFTERKPVIVSNDFYRRHYLYLSILWMPDSYTQGSLSVITA
                     LEDGSFLPQVQIEREWLQGCSLGMTTRMPLDMYTISSGHYGELGSTNLGFLIESTFFL
                     KVRF"
     gene            2743..>3510
                     /gene="pyrF"
                     /locus_tag="PKH81_02345"
     CDS             2743..>3510
                     /gene="pyrF"
                     /locus_tag="PKH81_02345"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNQ96908.1"
                     /translation="MQRLQAISREKGPLCVGLDTDPSYLPPEIIKLYDSPQDAVLAYN
                     LSLISTLAPYTACYKVQIAYYEAMGLSGLSVYSKTLKAIRKSGIPVIADIKRGDIADT
                     ARAYARAHFTGEFEADIITINPYMGFDTFEPFLEYVEKAGKGAFVLLRTSNPGMKDIE
                     WLPVTSETDADKPTRVLDVVGSALQSLVQRFPSPDKGCSPFGAVVGCTEESDARSLRD
                     RYPDLFFLIPGYGVQGGAARVAATLLDQSGGVVNSSRG"
BASE COUNT          965 a          617 c          808 g         1120 t
ORIGIN      
        1 tttctgataa atactggagc ccagaaatag tcggtatatt tcgatttgat tatcgccgtt
       61 ttgatgagga tgccatatta gtgtcgattg atcgactcca acgaatactg ggccttacac
      121 aatctattca acagcttgtt atctatacag actcgccaga ttctcttgta tcgattgagc
      181 agaatatcaa gaggaaattg ggagaactta ctattgtaac aagttggact gataattatt
      241 gggtcgcaat gtttcgttcg atgtctggaa tgttcttaat agtgttcgca gtatttcaga
      301 tagtggccag ttttttgata atcaacacta tggttatgat tattcacgag cgaataaaag
      361 agatcggcat gatgggtgct ttgggtatga acagaacaga aatcgtcatg gtttttttcc
      421 ttgaagctct gatgttgagt gtcctgggat cgttagttgg aatgataata ggaggcctga
      481 gctcctggat tgcatcatta tatcccctgg acatgaacac atttaccgga ggaggtatga
      541 aggatcttcc tatggcagga actttatacc tggctttttc ctggataacg tgtatcaaag
      601 gattcttgtt tggaatcggg gtgtctgctc tgtgcacact cttcccgtca atgaagagcg
      661 cttttataga acccgttgag gccttgcgtc ggtaaacaaa tgagagagaa ataaggaggt
      721 ttttgatatg aagagattac gaaatgctct gtccctcgga attatgatag cgagtatttg
      781 tctgagtgcg tatgcacaaa cagctcagga gattctttcc ctcgtagatg ataatgagat
      841 atatagttcc atcagttatg aggctgaaat gcacataagc tatcaaggca agacttttat
      901 taaaaaaatc agaggatatg gacgggaaaa taaggatagt tttatagaat ttataaatca
      961 ggaagatgta gggacaaagt atctaaaaaa agatggacga ctttacgtgt attctccaga
     1021 tacagaacag gtaatgctta tttctgggca catgcttaag gaatcgatga tgggctctga
     1081 ttatagttat gaagatacta tcgaaaacga tcgcttgtcc aaaaggtata gctcggttgt
     1141 ttctggagaa gaaatacttg gggacagagc atgttggatt ttagatctta cagcactaaa
     1201 atcgacagaa agctatccta cgcaacggtt gtgggtatcg aaagataccc atgaagttct
     1261 gcggtttgat ttatttgccc tatccggggt gcgagttaag gaatatagct tgttggaatc
     1321 gagattgatt aaaaatcgtt actttccggt gaaaagtgag atgagagatt taatgcgcaa
     1381 ggggagtaaa acagttttta caatggataa ggtcgaattg gatcaaaaat tatcagattc
     1441 actgttttct acaaggaatt tacgatgatt aagattccta tcacacatac ggcaatattg
     1501 gtttgtatgt ggattcttgt aattgctctg tccccaatct actcggaaac tgttatttcc
     1561 ggtgtggctg agacaaaagg cctgggacga ataagcgatg attcttatta tgtgcaagca
     1621 gaacagtttg ctaatatcag aatgcgaagt accatcggag agtatggaga atttcatagt
     1681 gcggtgcaat taattgtttc ctcgaataca gagaatgagt tttgctcaca agcggaacta
     1741 gaaagattgt atctttctct tgtgatagag agatttgata taaagcttgg tctccagcga
     1801 ttgcctttcg ggtatgggtt tgcttttaga ccattggata tgtttactca gccgagtgtt
     1861 ctatatccag aagctcggcc aaaaggagtt ttatcatcat tagtcaccgt atacccacgc
     1921 gatgaattat ctttttcttt tttttgtggg caacaacgcg atgatgtaat ccaggatact
     1981 cgtgatcctg tattttatgg acttgcgtta actcaacatc actctgtttt tagtacacag
     2041 tttttgtatg cactatcagt gtcccgtgat gaaacgcagc ctactactgg aagtggaggc
     2101 ttttcaacca aagttgacag cctgatcggt ataatattag aagcattgta tacacatgat
     2161 tactcttcgc cgtttgaaga ggggtctgta gatcacaata tacaagtgac agcgggtttt
     2221 gactattctt ggatgtcggg aaaactgtat ttggtaaccc aatattgtta ttcgagcgca
     2281 gggtatcttg attcaagcga ttctatcagc agtttgtata ctgatcctct ttgggccgat
     2341 cttccattta ctgaacgaaa accagtaata gtatcaaacg atttttaccg gagacattat
     2401 ctgtatttgt ccatattatg gatgcctgat tcatatacac agggttcttt gtcagttatt
     2461 actgctttgg aagatgggtc gtttttacct caggttcaga ttgaacgtga atggttgcag
     2521 ggatgttcgc ttgggatgac aaccaggatg ccattggata tgtatacaat atcatccggg
     2581 cattatgggg aattaggttc aacaaatctc ggctttttaa ttgagtctac gtttttcttg
     2641 aaagtcaggt tttaatcgtt ttttacaatg ctctgtccct ccatatggaa tagcgcttca
     2701 tctttatgtt attatttact catgaacaca atgatgtatg aaatgcagcg tttacaagca
     2761 ataagtagag aaaaaggacc tttgtgtgtc ggccttgata cggatccttc ctatttacct
     2821 ccagaaataa tcaagcttta tgatagtcct caagacgcgg tattggctta taatctttct
     2881 ttgatttcta ctctagcccc ttatactgcc tgttataaag tgcaaattgc ctattacgaa
     2941 gctatgggtt tgtcaggtct tagtgtgtac tcgaaaacac ttaaggcaat tcgaaaaagt
     3001 ggaattcccg tcattgctga tattaagcga ggtgatattg ccgacactgc acgggcatat
     3061 gctcgggctc attttaccgg tgaatttgaa gcagacatta tcactattaa tccttatatg
     3121 gggttcgata cctttgagcc tttccttgag tatgttgaaa aagcgggaaa gggtgctttt
     3181 gttttattac gaacctcaaa tcctggtatg aaagacatag agtggttgcc cgttacctct
     3241 gaaactgacg ctgacaaacc gacaagggtt cttgatgttg tcggttctgc tttacaatct
     3301 ctggtacaac gctttccctc tcctgataag ggatgtagtc catttggggc agttgttgga
     3361 tgcacggagg aatcggatgc ccgttctctt cgtgatcgat atccggatct tttctttctg
     3421 attccgggtt atggagttca aggtggtgca gcacgtgtag ctgcaacctt gcttgatcaa
     3481 agtggtggtg tcgtcaattc atcccgtggg
//
LOCUS       DAOQXQ010000096         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28066_length_3492_cov_2.867326, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000096 DAOQXQ010000000
VERSION     DAOQXQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28066_length_3492_cov_2.867326"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PKH81_02350"
     CDS             <1..1087
                     /locus_tag="PKH81_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96909.1"
                     /translation="VSSEERAPEGATPIILETRITDGKAFVPIPFAFYNSNEGVMAGV
                     LGNAPNVRGSMQDVLFTGLYSAPPDQNDDLQWTDPNFILLLNWSGILLYPPFSLGFFT
                     SIMKMNRKIEDRGEVLAKYNALSLIGTINLSIPIGQSAENTFSFGGRHILTNKVEFAE
                     TDESLAYGPYDWTITVKDTFSIKRFNWRGHFREGWKGQLEAEWEFIDPTYSACRTDVT
                     LSGEASIFRIIGTRFNPQARIAAFTNTDLPHLDVASKVRGLRNGELKANTAITANTNV
                     QILLARIGGTEIHLSPGFDIALAWTPEEDEREYDWACAAGGELLLFFDSIKSLPVKFG
                     YAYDLRPPERNGSGKRYEIEFTFSLSY"
     gene            complement(1084..2169)
                     /locus_tag="PKH81_02355"
     CDS             complement(1084..2169)
                     /locus_tag="PKH81_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96910.1"
                     /translation="MKRSLRLTFSLALLLCGLGLVYGAERNPSFPEPLHGEWVLYRDY
                     SWKDPTWVGFMYYDDSAWAALLVTPATGTRVSILFRTENADGTMILLGQRNLGSPTPD
                     DTLPINYLMGLLPDLHAWRYEALSSSAKKDRGAHKGRSSLLPPAMVSRRDIRSFGGAV
                     SLLWKPEIAAFNLDSITASDGSPRLRLALQGRISSGSDNDFFTLEPPTDQAVQTRPAA
                     DITLQPSKNREKRVVDGVTLSLDSSWTMIADNTFFMGNEAMLIVDTLDVRLMGLASVN
                     LPLEMLRLFSRSGKDAVMDVSSHALSGSAKRFRIESVVRDRQTGRVNRDIKVCIPQSD
                     GAKCALVSLSVSDDFYQANKKYFESLF"
     gene            complement(2166..>3492)
                     /locus_tag="PKH81_02360"
     CDS             complement(2166..>3492)
                     /locus_tag="PKH81_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNQ96911.1"
                     /translation="HTDDASLDETYQEMGRAYRRIFRRCGLSVIPVRADSGAMGGTGS
                     EEFMVESEIGDNNLILCPSCGYAANDEKAGCAPDPLTSGPGSAPATAPYAEIATPAVR
                     TIDELCAFLKTTSKAFIKTLIYRAINCELDISGAPGAASLKRVQPESAAPYYPEAFFA
                     VCIRGDLDVNEVKLAAALKASEVELASDSDVERITGAPVGFAGPVGLSSVPVVADLSV
                     MAMHDSVTGALKADAHFEHVEPLRDFTPFLSADVRTVVAGDKCASCGATYYAKKGNEL
                     GHIFKLGYKYTRSMGMNYLDENGKQQTPTMGCYGIGVDRTLASIIEENHDDDGIQWPM
                     SVAPFQVCIVPIKYEGAMKEAADSLYNELSARGVEVLLDDRDERPGVKFKDMDLIGIP
                     LRVVVGDKNLPNVEVKKRGEKDANLMPQAEAAKALVTMVSEALAALRAV"
BASE COUNT          871 a          934 c          927 g          760 t
ORIGIN      
        1 agtctccagc gaggaaagag ctcccgaggg agcaacgccc ataattctgg aaactcgtat
       61 aacagacggc aaggcctttg ttcccattcc ctttgccttt tataacagca acgagggcgt
      121 tatggccggc gttctgggca atgcgccaaa cgtccgtgga agcatgcaag acgttctgtt
      181 caccggcctc tatagcgctc cccccgatca aaacgatgac ctgcaatgga cagaccccaa
      241 cttcattctt cttttaaact ggtcaggaat actcctttat cctccgttca gccttggctt
      301 ttttacctct atcatgaaaa tgaaccggaa gatagaagac cggggagaag ttcttgccaa
      361 gtacaacgca cttagcctga ttggaaccat taacctaagc atccccatag gccaatcggc
      421 ggaaaacacc ttttcctttg gcggtcgaca cattttgacg aacaaggttg agtttgcgga
      481 aacagacgaa tctcttgcct atggtccgta tgactggacc ataacagtaa aggatacctt
      541 ctccatcaaa cgatttaatt ggagaggaca cttcagggaa ggttggaagg gacagctgga
      601 ggctgaatgg gaattcatcg atccaaccta ttctgcctgc cgaaccgatg ttaccctttc
      661 cggcgaagcc tctattttcc gcatcatagg aacccgcttt aaccctcagg ccagaatcgc
      721 agcctttacc aataccgact taccgcacct tgacgtggca tccaaggtgc ggggcttacg
      781 aaacggcgag ctcaaggcga atacagccat taccgccaat accaatgtcc agattcttct
      841 ggcccgaatc ggcggaaccg agatacatct atcccccggc tttgacatcg ccctggcatg
      901 gacgcccgaa gaagatgagc gggaatatga ctgggcatgt gcggcgggag gcgagctcct
      961 cttgttcttt gatagcataa aaagcttgcc cgtaaaattc gggtacgcct atgacctgcg
     1021 tccgcctgaa cgcaacggta gcggcaagcg ttacgaaatc gagtttacct tcagtctgag
     1081 ctattaaaac aaagattcaa agtacttctt gtttgcctgg taaaagtcgt cagacaccga
     1141 aagacttacc aaggcgcact ttgccccgtc tgactgagga atacacacct taatatcccg
     1201 atttacccgg ccggtttgcc ggtcgcgaac gacagattca atgcgaaacc gcttggcact
     1261 tccgctaagc gcgtgagacg agacatccat tactgcgtcc ttacccgaac gggaaaaaag
     1321 gcgaagcatc tccaggggaa gattaacaga ggcaagcccc atcaaacgaa catccagagt
     1381 atccacaatc agcatagcct cgttgcccat aaaaaatgtg ttatccgcaa tcatggtcca
     1441 cgagctatcc aaagaaagtg ttacaccgtc aacaactcgt ttttccctgt tcttggaagg
     1501 ctgcagggta atatccgccg cagggcgggt ttgaacggct tggtctgttg gcggctcaag
     1561 ggtaaaaaag tcattgtcag aaccggaaga aatacgtccc tgcaaggcaa gtctcagccg
     1621 gggagagccg tcggaagcgg taattgaatc aagattaaaa gcggcaatct caggcttcca
     1681 aagcaaagaa accgctccgc caaaggagcg gatatcccgg cgggaaacca tggcaggagg
     1741 taataaagat gagcggccct tgtgcgcgcc cctgtctttc tttgcggaag aactgagggc
     1801 ctcgtatcgc catgcatgga ggtcgggcaa gagccccata aggtaattta tcggtaaggt
     1861 gtcgtcgggt gtgggtgagc caagattacg ctggccaagc aatatcatcg taccatccgc
     1921 gttttcggtg cggaacagga tggacacccg cgttccggta gcgggcgtaa ctaaaagggc
     1981 agcccaggca gaatcgtcgt aatacataaa tcctacccag gtaggatcct tccatgaata
     2041 gtcgcgatag agcacccatt caccatggag cggttcagga aaggatggat tacgctccgc
     2101 tccatagaca aggcccaggc cgcacagtaa cagggcaaga gaaaaggtca ggcgcaagga
     2161 gcgcttcata cagccctcaa tgcggcaagg gcttcgctca ccatggttac aagagctttg
     2221 gctgcctctg cctgtggcat aaggtttgcg tctttttccc cgcgcttttt aacttcaacg
     2281 tttggcaaat ttttatcgcc cacgacaacg cgaaggggaa taccaattag gtccatgtcc
     2341 ttaaacttga ctcccggtcg ctcgtcacgg tcgtcaagca agacctcaac gcccctggcg
     2401 ctcagctcgt tgtacaggga gtcggccgct tccttcatgg cgccttcgta cttgatagga
     2461 acgatgcata cctggaaggg tgcgacgctc atgggccatt gaatgccgtc atcgtcgtga
     2521 ttttcctcga taatcgaggc aagggtgcgg tcaaccccaa taccgtaaca gcccatggtg
     2581 ggagtttgct gcttgccgtt ttcgtccagg taattcatgc ccatgctgcg ggtgtattta
     2641 taaccaagct taaagatgtg accgagctca tttcccttct tggcgtaata agtggctccg
     2701 caagaggcac acttgtctcc ggccacaacg gtgcgtacat cggcggaaag gaagggggta
     2761 aagtcgcgta agggttctac gtgttcaaag tgggcatcgg ccttaagcgc gccggtaaca
     2821 ctgtcgtgca ttgccataac ggaaagatcg gcaaccacag gaacggagga aagaccgaca
     2881 gggccggcaa agccaaccgg cgcgccggtg attcgctcaa cgtcactatc gcttgcaagc
     2941 tcaacttcgc ttgccttaag ggcagcggca agctttacct cgtttacgtc aaggtctccg
     3001 cgaatacaca cggcaaaaaa ggcctcgggg taatatgggg cagcactttc cggttgcact
     3061 cgcttgagcg aggctgcacc cggggcgccg gagatatcca gttcgcagtt gatggcgcgg
     3121 tatataaggg tcttgataaa ggcctttgaa gttgtcttaa ggaaggcaca gagctcgtca
     3181 atggtgcgca cggcaggggt tgcaatctct gcataggggg cggttgcagg ggcactgccg
     3241 ggcccggagg taagcggatc aggggcgcac cctgccttct cgtcgttcgc agcatagccg
     3301 caagatggac aaagaataag gttgttgtcc cctatctcgc tttcgaccat aaactcctcg
     3361 ctgccggttc ctcccatcgc gcctgagtcc gcacgtacgg gaatgacaga aagtccgcaa
     3421 cggcggaaga tacgccgata cgccctgccc atttcctgat aggtttcgtc aagggaggcg
     3481 tcgtcggtat gg
//
LOCUS       DAOQXQ010000097         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28070_length_3492_cov_2.285132, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000097 DAOQXQ010000000
VERSION     DAOQXQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28070_length_3492_cov_2.285132"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..803
                     /locus_tag="PKH81_02365"
     CDS             33..803
                     /locus_tag="PKH81_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ96912.1"
                     /translation="MIETADTDDCIDFVFFRATNGCTGKTIAKDTAAIRSFFRYLVIE
                     RVRSDNPADLLESPRREKTLPRVLTPVQIDDFLDAIPLDTDYGIRDRCLFELIYSCGL
                     RVSEAVSLSLVDVRLKERLLLVHGKGGKERLVPFGEAARLQITRYLQDIRPKILGKKR
                     NDAVFLNYRGGRLTRKGIWKRFHEIILSQGLESKIHTLRHSFATHLLAGGADLRSVQE
                     LLGHADVATTQIYTHIENSALQMYHADFFDNYQAETDN"
     gene            828..1562
                     /locus_tag="PKH81_02370"
     CDS             828..1562
                     /locus_tag="PKH81_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ96913.1"
                     /translation="MTVNIWSRILMLGGFSLFFLSCGPNTNYIRRMQALEEGVDNPTS
                     IEELEDAIKKYQHRIEDVLNADIRIGMWYKILATRYLDNGMHAKALENFQTAIQYYPT
                     NQNLYYYVGVCAGYMAKSALDFEATGTSSKSSNYYQLAESAYVRAIELEPRYVRALYG
                     LSVLYVFELDRPADAIPHLQLVAEIETRNTDVRFILARAMYMTGDIEGAVAAYDSILS
                     ISRDEKTKETARQNKAEVLQRGYGSD"
     gene            1549..2460
                     /gene="dprA"
                     /locus_tag="PKH81_02375"
     CDS             1549..2460
                     /gene="dprA"
                     /locus_tag="PKH81_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ96914.1"
                     /translation="MDQIELTLGLSCCSFLSCKDIRILREKLDNLEDLTVLSIEEICR
                     LVGRRIQTGLWNPSTLRSRVDFCRTLMERYRISMTTIDESDYTPLVREIFDPPFAIFW
                     RGLLPDPEQAIVAMVGTRSPTGVGIRLAERIGRDLGERGIPVVSGLARGIDAFSHKGN
                     CDGGGKSIAVLACGLERIYPKGNSLLAGRLLDKGGCLIGEYPPGDPPLTFHFPQRNRL
                     ISALARSVVVIEAPKRSGALITADFALEQGRDLFVARDALHTARAEGLRMLREQGAPA
                     ISSAGDIIESWDSNYAACVTKQLALDF"
     gene            2497..>3492
                     /locus_tag="PKH81_02380"
     CDS             2497..>3492
                     /locus_tag="PKH81_02380"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNQ96915.1"
                     /translation="MATKTASSKKKAAKNTLVIVESPAKAKTIEKYLGRGYTVKASMG
                     HLIDLPKSRMAVNVEDNYSPEYITVRGRAKLLKELQSDAKKSKEVLLASDNDREGEAI
                     AWHLRNSIQAKYQVPIRRIVFNEITPQAIKDAVQHPMEIDESKVNAQKARRVLDRLVG
                     YNLSPLLWKKVKNGLSAGRVQSVALRLICEREAEVENFIPTEYWTLDADFHKGRVDFT
                     AQLVQYKGEKPELVNEDAVNSIIHDIKSSTSSIVDIKTTEKTIRPKAPFTTSKLQQMA
                     ANRLGFTSKKTMQIAQQLYEGINIGSTRVGLITYMRTDSVRISATALEEVRSWIGE"
BASE COUNT          991 a          679 c          844 g          978 t
ORIGIN      
        1 tattctgtca atggcttcaa agtcgctctc tcttgattga aacagctgat accgatgatt
       61 gtattgattt tgtttttttt cgggccacaa atggatgtac cggaaaaaca attgctaagg
      121 atacggcagc catcaggtct ttttttaggt atttggtaat tgaacgggta agaagcgata
      181 atcctgcaga tcttttagag tcccctcgta gagaaaaaac acttccaagg gtattaactc
      241 ccgttcaaat cgatgatttt cttgatgcaa taccccttga taccgattat ggaatacgag
      301 atcgttgttt atttgaactg atatactcct gtggtttacg ggtgagtgag gccgtgtcgc
      361 tgtccctagt tgatgtacgt cttaaggagc gattgcttct tgtgcatggg aagggtggaa
      421 aagagcggct ggtccccttt ggagaagcgg cccggctcca aataactcga tatctacaag
      481 atattcgccc taaaattctg ggaaaaaaaa ggaatgatgc ggtttttttg aattatcgag
      541 ggggtcgttt aacgcggaag ggaatttgga agcgatttca tgagattatc ttgtctcaag
      601 gtctagaatc taaaattcat acgctcaggc attcctttgc aacacattta ctggcggggg
      661 gtgcagattt gcgatcagtt caggaattgc tcggtcatgc agatgtagcg acaacacaga
      721 tatatactca tattgaaaat agcgcattac agatgtatca cgcagatttt tttgacaatt
      781 atcaggcaga aactgacaat tgattgatag ataaggagag gattattatg actgttaaca
      841 tctggtcaag gattcttatg ctcggtggat tttcgctttt ttttctatct tgtggtccca
      901 atactaatta catacggcgg atgcaagccc ttgaggaagg ggtagacaat ccgacttcaa
      961 tagaagaatt ggaagacgcc attaaaaaat accaacatcg catagaggat gtgcttaatg
     1021 ctgatatccg tattggtatg tggtacaaaa tactggccac acgctattta gataatggta
     1081 tgcatgctaa agcattagag aactttcaaa cagccattca gtattatcca accaatcaaa
     1141 acctctatta ttacgtgggt gtttgtgccg gatacatggc aaaaagtgcg cttgattttg
     1201 aagccaccgg aacttcttct aagagttcta attattatca acttgccgaa agtgcttatg
     1261 tgcgggccat agagctggaa ccgcgatatg ttcgtgcctt gtatggactt tctgttttat
     1321 atgtttttga acttgatcgc ccagctgatg ctatacctca tttacaattg gttgccgaga
     1381 tagaaaccag gaataccgat gttcggttta tcctggctcg agctatgtac atgaccgggg
     1441 atattgaagg tgccgtagcg gcctatgatt ccattttatc gatatctcgg gacgagaaaa
     1501 caaaggaaac agcccgtcaa aataaagcgg aggttctcca gcgtggatat ggatcagatt
     1561 gaactgacct tgggtttatc ctgttgttca ttcctttctt gcaaggatat tcgtatattg
     1621 cgggaaaaac ttgacaatct tgaagacctt acggtacttt caatagaaga aatttgtcgt
     1681 ctggtaggta ggcgaataca gacaggcttg tggaatccat ctacccttcg ctcccgggta
     1741 gatttttgca ggaccttgat ggaaaggtat cgcatatcga tgactactat tgatgagagt
     1801 gattatacac ctcttgtccg agagattttt gatcctccct tcgctatttt ttggagaggg
     1861 ttgcttcctg accctgaaca agcgatagtt gccatggttg gaacgcgaag tcccaccggt
     1921 gtggggatac gattggctga acgtattggc cgagatttgg gagagcgggg tatccccgtt
     1981 gtatcaggcc ttgcacgggg tattgacgct ttttcccata agggtaactg tgatggcggg
     2041 gggaaatcaa ttgccgtttt ggcatgcggt ttggagagga tttatccaaa aggcaattcg
     2101 ttattagctg gaagactttt ggataaaggt ggatgtctta taggggaata tcctccaggg
     2161 gatcctccct tgacctttca ttttccccag cggaaccgtc taatatccgc tcttgcacga
     2221 tctgtagtgg ttatagaggc cccaaagcgt tctggagccc tgataaccgc agattttgct
     2281 ctggagcaag gacgggatct gtttgtggca cgggatgctc ttcatacggc ccgggcggag
     2341 ggcctacgga tgttgaggga acagggagcc ccggctattt catcagccgg ggatattatt
     2401 gaatcttggg attcaaacta cgcggcatgt gttaccaaac aacttgccct tgatttttaa
     2461 aatgtttcct tgaatggata gataggagta ctgttaatgg caaccaagac agcatcaagc
     2521 aagaaaaagg ccgcaaagaa tacactggta attgtagagt cgccagcaaa ggcaaaaacc
     2581 atagagaaat atcttggacg aggttatacc gtaaaagcgt ctatgggtca cctgattgat
     2641 cttccaaagt cacgaatggc tgttaatgtg gaagataatt attccccgga atatattacc
     2701 gtacgggggc gtgcaaaatt actgaaagaa ttgcaatcgg atgctaaaaa atcaaaagaa
     2761 gtgttactgg caagtgataa tgaccgcgag ggagaagcca tagcatggca tttacgaaac
     2821 tccatacagg ccaagtacca ggttccgatt agaagaattg tatttaacga gattacaccc
     2881 caggcaatca aggatgctgt tcagcatccc atggagatcg atgaatccaa ggtgaatgct
     2941 caaaaagctc ggcgagtact tgatcggctt gtaggataca atctgtcgcc cctactatgg
     3001 aaaaaagtaa aaaatggttt gtcggcagga cgggttcaat cagtagcact gcgtctcatt
     3061 tgcgagcggg aagctgaggt tgaaaacttt atccctacag aatactggac tcttgacgct
     3121 gattttcata agggaagagt ggattttacc gctcaactgg ttcaatataa aggtgaaaaa
     3181 cccgaattgg ttaacgagga tgcggttaat agcataatcc atgatattaa atcaagcaca
     3241 tcttcaattg ttgatataaa aaccactgaa aaaactattc gaccaaaggc tccgtttacc
     3301 acatcaaagc ttcaacaaat ggcagcaaac cggcttggat ttacctccaa aaaaacgatg
     3361 caaattgccc aacagctcta tgaaggcatt aacattggtt caacacgtgt tggtttgatt
     3421 acttatatgc gaacagattc agttcgtatt tcagctacag ccctggaaga agttcgatcg
     3481 tggataggag ag
//
LOCUS       DAOQXQ010000098         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28079_length_3491_cov_2.996799, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000098 DAOQXQ010000000
VERSION     DAOQXQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28079_length_3491_cov_2.996799"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKH81_02385"
     CDS             <1..749
                     /locus_tag="PKH81_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNQ96916.1"
                     /translation="WAASKKLTKATGVRCQPNKAIVGENAFAHEAGIHQHGVLANRAT
                     YEIMTPESIGLPHNKMVLGKHSGRHAFEERLAYLGHRVEEARLNTLFEEFKVLADKKK
                     TVSDRDIDAMVSGASGGVPEAYKLDRFVVNSGSSITATSVVRLLMRDGSKVEKVSIGD
                     GPVDASLNAVDKIIGGSEIKLEDFNLQIVDATDTGADAQGESHVRITRDGRTWNGSGV
                     STDIVESVLKAYLAAINAMTFELEQERMRG"
     gene            756..2362
                     /gene="cimA"
                     /locus_tag="PKH81_02390"
                     /pseudo
     CDS             756..2362
                     /gene="cimA"
                     /locus_tag="PKH81_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
     gene            2467..>3491
                     /locus_tag="PKH81_02395"
     CDS             2467..>3491
                     /locus_tag="PKH81_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNQ96917.1"
                     /translation="MGMTMTQKILAAHAGLDSVVAGQLIEAKLDLVLGNDVTTPVAIN
                     EFRKIGCSAVFDKTKIAIVPDHFTPNKDIKAAEQAKLVREFAREKEIENYFEVGCMGI
                     EHALLPEKGLVVAGDVVIGADSHTCTYGALGAFSTGVGSTDMAAGMAAGTAWFKVPSA
                     IKFVLNGKLSGWASGKDVILHIIGMIGVDGALYQSMEFTGPGVATIDIDGRFTIANMA
                     IEAGAKNGIFPVDKVALKYLKEHGKRPSKIFEADADAVYDRVIEIDLAQIKPTVAFPH
                     LPSNTKTIDQAKGLKIDQVVIGSCTNGRIEDLRIAAKILKGRKVSKDIRCIIFPATQK
                     IYLQAMK"
BASE COUNT          899 a          794 c          920 g          878 t
ORIGIN      
        1 tgtgggccgc gtccaaaaag cttaccaagg caaccggggt gcgctgtcaa ccaaacaagg
       61 ccatcgtagg cgaaaacgct tttgctcatg aggctggaat tcatcagcat ggggtactgg
      121 ccaatcgtgc tacctacgag attatgactc cggaatcaat aggtttaccg cataacaaga
      181 tggtgctagg aaaacattca gggcgtcacg cgtttgagga gcgtcttgcg tatttgggtc
      241 atcgggtgga ggaagcgcgc cttaacacac tttttgaaga gtttaaggtt ttagcagaca
      301 agaaaaagac cgtctccgat cgcgacattg acgcgatggt ttcaggcgcc tctggcggag
      361 tgcccgaggc ctataagctt gaccgatttg tggttaactc aggatcttct attaccgcaa
      421 cctcggtggt tcgtctgctc atgcgtgatg gctcaaaggt tgaaaaggtc tccattggcg
      481 acggtccggt agacgcatct cttaacgcgg tagacaagat tatcggtggt tcggaaataa
      541 aattggaaga ctttaatctg caaattgttg atgccaccga cacgggtgcc gacgcgcagg
      601 gagaaagcca cgttcgcatt acccgcgatg gacggacctg gaatgggtcc ggggtttcta
      661 cggatattgt agaatcggtg ctcaaggcct atcttgcggc gataaacgcg atgacctttg
      721 agcttgagca agaaaggatg cgggggtaac acgtgatgac ggatgataaa ggattggtta
      781 agactctaca tatatttgat tcaacccttc gcgatggcgc gcagggagag ggtattagtt
      841 tttccgtaca ggataagtta catatcgtta gcgcccttga tgatttaggg gttgcctata
      901 ttgaggcggg aaatccaggg tctaacccca aagacctgga gttttttaga gaggtaaaaa
      961 agatctcgct taaaaactcc cgcctcgttg cctttggctc cacacgccgt aagggaatcc
     1021 ttgccaaaga cgatggaaac ctcaaaagcc tgttggatgc cgacaccgaa acggtgtgta
     1081 tcttcggaaa gacctgggac tttcatgttg ccgagattct taaggctacg ccggaggaaa
     1141 atctggagat gattagggac accgtatctt ttcttgtaag tcaagggcgt actgtcatct
     1201 atgacgctga acacttcttt gatggcgcac tcgccaatcg ggagtacgcc atgagtaccc
     1261 tcaaggcagc agtcgatgga ggggccgcaa ttcttgtttt atgcgataca aacggtggaa
     1321 tgcttcctga tatggttcgc tccatgaccg aggatgtcgt tcgcgccttt ggtatccctg
     1381 tgggcattca ttgccacaac gacactggta tggctgtcgc gagctccctt ctttcggtaa
     1441 agggtggagc tattcaggta cagggaacct tgcttggctt tggcgagaga acgggtaacg
     1501 caaatctttc cacaattatt gcgaaccttg agctcaagat ggctcttccc tgtcttcccg
     1561 aaggaaaaat tgcaaccctt acccatacag cacgacgtat agcggagata tcaaatatcg
     1621 ttttggaaaa cggcatgccg tatgtcggtt ttaatgcatt tgctcataaa gcaggtatgc
     1681 atatcgatgc agtaactaaa aatccacggg cctatgagca tgtcgcaccc gattcagtag
     1741 gtaacgagcg taccttcttg atgagcgagg ttgccggtcg atccttgatc atagacaagc
     1801 taaaaaaatt cgacgaatcc attaccaaag attcgccggt agccgcgcaa attgtggcgc
     1861 gggtaaaaga actcgagcac gctggctatc aattcgaggg agccgacgga agttttgatc
     1921 tattggttca taagaatatg ggaacctata tccgtatttt aaccttcact attacaaggt
     1981 tattggagag caaccaattc ctggtgttcc ctataatgcc tttgctcaaa taaagataga
     2041 tgttggttct gcagtcgcga taaccgcagg agaaggggat ggtcctgtcc atgcacttga
     2101 cgttgctcta cgaaatgccc tggagcgatt ttatccggtt gttgccagta tccgactcac
     2161 cgactttaag gttcgcgtgc ttgactcaag aagcgcaact gcagcaaagg tcagggtatt
     2221 gattgagtcc acagatggcg aagaatcttg gacaaccgtc ggggtttcaa gcgacgttat
     2281 cgaagcctcg tggatagccc ttgtcgattc tttcgagtac aagttgatta cctccggaga
     2341 aaagcatccg gaaacgacat aataggggtt ctggactctg gacagcaatc tttaattata
     2401 ctaaagttat tacagtatat gtttagatgc gtcggttata gataattcga cgaaaaggag
     2461 aaattcatgg gaatgacaat gacccagaag attctggccg cccacgcggg actcgattca
     2521 gtcgtcgccg gtcagctcat cgaggcaaaa ttggacctgg tgcttggaaa cgacgttacc
     2581 actccggttg ccatcaatga attcaggaag ataggctgtt ctgccgtttt tgacaaaaca
     2641 aagatcgcca tcgttccgga tcactttacc ccgaacaagg atattaaggc cgcagaacag
     2701 gctaagctcg ttcgcgagtt cgcccgcgaa aaagaaattg aaaactactt tgaagttgga
     2761 tgcatgggta ttgagcatgc cctgcttccc gagaagggac tggttgtagc cggtgatgtg
     2821 gttatcggcg cggatagcca tacctgtacc tatggagccc tgggcgcttt ttctaccggt
     2881 gttgggtcta ccgacatggc tgccggtatg gccgcgggta ccgcctggtt taaggttcct
     2941 tccgcaatca agtttgtact taacggaaag ctttccggtt gggcaagcgg taaggacgtg
     3001 atccttcata ttatcgggat gatcggtgtt gacggggcct tgtaccagtc aatggaattt
     3061 accggtcccg gcgttgctac catcgacatt gatggccgct ttaccatcgc gaatatggca
     3121 atcgaggcgg gcgcgaaaaa cggaatcttt ccggttgata aggtcgccct taagtatctt
     3181 aaggaacacg ggaaacgtcc ttcaaagatt tttgaagcgg atgctgatgc agtatacgat
     3241 cgagttatcg agattgatct tgctcaaatc aaaccgaccg ttgcctttcc ccatcttcca
     3301 tccaacacca agaccatcga tcaggcaaaa ggtcttaaga tcgatcaggt tgttatcggt
     3361 agctgtacca acggacgcat cgaagacttg cgcatagctg cgaaaattct gaagggccga
     3421 aaggtttcca aggatattcg ctgcatcatc ttccccgcaa ctcaaaagat ttaccttcag
     3481 gcaatgaagg a
//
LOCUS       DAOQXQ010000099         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28442_length_3461_cov_2.103347, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000099 DAOQXQ010000000
VERSION     DAOQXQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28442_length_3461_cov_2.103347"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PKH81_02400"
     CDS             complement(<1..1185)
                     /locus_tag="PKH81_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96918.1"
                     /translation="MEKKVACALILILTLLAPLVARGNPEVGLPKVDRLIKERNYNAA
                     IMELAEYMTETPEDFDGAQRRVRRIINMREDYNDLGLALLGVLTNEPTNDKKKLDMIT
                     YLESLEKNPNPSTQAFIAEIKSAAQFTYYRAKFDEIMNDGNSLIDQGLYVEAARKFSE
                     GYTFYKTEFDEENPEQLVSDVNARLARVSSLLDSYALIQESLSTLSTASQTALATGDH
                     AVALVSLKGLEGELASLARLRNTVAEQGWFFSDTFDASNGKNAQTTDNSFLPFAWRFT
                     LGRKTIGRFEGVLGAMDAQWDKTLSEVEEATEQTVWRNWQSAYEHLGSGNVEAARANL
                     FEARRFASLARSFPAVSDQLQRRADTWGMRDPAVPRTRYTSVDTLISAMGELGDIYTR
                     FLA"
     gene            1291..2562
                     /locus_tag="PKH81_02405"
     CDS             1291..2562
                     /locus_tag="PKH81_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HNQ96919.1"
                     /translation="MDMRFRILFVSLAFSMYTVVCTSALDLSDPYSYVSGIFEPLIDE
                     NEGLTTFRSLLIPGGGRGEAMGSAFSALANDISFFEANPAGSSTMKNTELALFHNNWI
                     ADSKLETLSFTTRMENLGYGVSIRSFYVPFTEYNTFGERVSRGYYSESLATFNVSYNF
                     LAGYYFKGIAVGFNLKTGYRSVPDYSDNYGNLQEDSGSDQSAFTIMADLGFQTRFNLL
                     KLYNSRDPNFFVGATFRNFGPPVLDEPLPSLITAGAAYKPANPITISAEIQKPINLVD
                     PELSGSMVYGAGLMVDMASFFSFMAGLQLKGANPRISIGGEVNVNDIQFNINYTLDMT
                     TQLALLNRISLSAKLNLGDRGRGAKARAVEELYIQGLRLYALGELENAIAVWKEVLLM
                     DRRFDPAIEGIETASITLELQKRIRDLQQIE"
     gene            complement(2578..>3461)
                     /locus_tag="PKH81_02410"
     CDS             complement(2578..>3461)
                     /locus_tag="PKH81_02410"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNQ96920.1"
                     /translation="LVFARDFFSLTDVTSTDSSFDQNRVYLDTERDVGFELSESLLSP
                     LFDVHQGRRITGFRYYAKSAENQDQGSLYDEGLARTQIEEDARQFIQRRFETLNKASS
                     LLEGAPVSICATYPASLFGQSWYEGPAWLEATFRQLAVHPELSAALPCQRAIPRQLSQ
                     PIEPYCSSGLQGGYAEDVLNSANDWMYPYIRKATLRMIDLAERFPDDNGLKERALNMS
                     ARELLLAQSMDWFIMMNDREHSDYAKRRFQESVRAFTVVYESLGSNFISTEWLTKIEK
                     VDNLFPEINYRVYSKKK"
BASE COUNT          923 a          843 c          790 g          905 t
ORIGIN      
        1 ggcgagaaac cgggtgtaaa tatccccaag ttcacccatt gcagatataa gcgtatcaac
       61 actggtataa cgagtgcgag ggacggcagg atcgcgcatc ccccaggtat cagcccttcg
      121 ttgtagctga tcagatacag ctggaaagga acgagcaagg gaggcaaacc gtctggcttc
      181 gaatagattc gctcgggcgg cttcaacatt accacttcca agatgctcat aggcgctctg
      241 ccagtttctc cacacggtct gttccgtcgc ttcctctact tccgaaaggg tcttatccca
      301 ttgtgcgtcc atagcgccaa gaacaccctc aaaacgaccg attgttttcc ggccaagggt
      361 aaaacgccag gcgaagggta aaaaggagtt gtcagtggtt tgtgcgttct taccgttaga
      421 agcgtcaaag gtatcggaga aaaaccaccc ctgttcggct acagtattgc gaagtcgtgc
      481 cagagacgca agctcgcctt ccaaaccctt aagagacaca agggctactg cgtggtcccc
      541 ggtagcaagg gcggtttgtg acgctgtgga taaggtgctc aaagattctt gtatcagggc
      601 atatgaatcc aataaggatg aaacccttgc aagtcgggca ttaacatcgg aaacgagctg
      661 ttcgggattt tcttcatcaa actcagtttt ataaaaggta tacccttcgc tgaattttcg
      721 ggcagcttca acgtatagac cctgatcaat caagctatta ccgtcgttca tgatctcgtc
      781 aaactttgcg cgataatacg taaactgggc agcactcttg atttccgcga taaacgcctg
      841 cgttgacggg ttcggatttt tttcaaggct ttccaggtag gtaatcatgt cgagcttttt
      901 tttgtcgttt gtcggctcat tggtaagaac acccaacagg gcaagaccaa gatcgttata
      961 gtcctcgcgc atattaataa tccgcctgac acgtcgttga gcaccatcaa aatcttccgg
     1021 tgtttccgtc atgtattcag ccagttccat aatagcggcg ttataattcc tttctttaat
     1081 cagacgatcc accttgggga gtcccacttc gggatttccc ctggcaacca gcggtgccag
     1141 tagagtaaga atgaggatga gggcgcacgc aacttttttt tccacaggct tcctgttgat
     1201 caactcggta cactattaat ttcggcagat aaaattgcag aatccagtac taaactgaag
     1261 aataatgccc gaaaatacaa tatactcact atggatatgc gttttcgtat tctttttgtc
     1321 agtctagcct tctccatgta taccgttgta tgcacatcgg cgcttgacct gtccgatccg
     1381 tatagctatg tctccggaat atttgaacca cttatcgacg aaaacgaggg gcttaccaca
     1441 ttccggtctc tgctcattcc tggaggaggc cggggcgagg cgatgggctc cgccttttcg
     1501 gctctcgcaa atgatatatc cttttttgaa gcaaatcctg cgggcagttc aaccatgaaa
     1561 aacacagaac tagctctttt tcataacaac tggattgccg attccaagct cgaaaccctc
     1621 tcctttacta cccgaatgga aaacctgggg tatggcgtat ccatcagatc cttttacgtc
     1681 ccctttactg agtataatac ctttggcgaa cgtgtatctc ggggatacta cagcgaatcc
     1741 cttgcgacat tcaatgtctc ttacaatttc cttgcaggat attactttaa gggtattgcg
     1801 gttggcttta accttaaaac aggataccgc agtgtccccg attattctga taattacgga
     1861 aatttacagg aagattcagg ttccgatcag tcggccttta ccatcatggc agatcttggt
     1921 tttcaaacac gttttaacct gctaaaactc tataattccc gagacccaaa cttttttgtt
     1981 ggcgcaacct tcaggaattt tggtcctcca gtccttgatg aacccttacc cagtctaata
     2041 acagcagggg cagcctataa accggcaaac ccgatcacta tttccgcaga aattcaaaaa
     2101 ccgatcaacc tggtagatcc agaactttcg ggttccatgg tatatggcgc agggcttatg
     2161 gtagatatgg caagtttctt ctcttttatg gctgggctcc aacttaaagg cgcaaatccc
     2221 agaattagca tcggtggcga ggtaaatgtt aatgatattc agtttaatat taactatacc
     2281 ctcgacatga ccactcaact cgccctgctc aaccgaatca gtctttcggc aaaattgaat
     2341 ctgggagacc ggggccgggg tgcaaaagcc agagcagttg aagagctcta tatacagggc
     2401 ttacgcctgt atgcccttgg tgagctggaa aatgccatcg cagtgtggaa ggaagtatta
     2461 ctgatggaca gacgctttga cccagcgatt gagggtatag aaacagcgag tatcaccctg
     2521 gaactgcaaa agcggattcg cgatttacag cagattgaat agacagaaaa aagagcctta
     2581 cttttttttg ctgtacacgc ggtagtttat ttccggaaaa agattatcaa ccttttcaat
     2641 cttggttagc cattctgtac tgataaaatt cgagcccagg gattcatata cgacggtaaa
     2701 ggctctaacg ctctcttgaa aacggcgttt ggcgtaatcg ctgtgttctc gatcgttcat
     2761 catgataaac caatccatcg attgagccaa aagcagttcg cgtgcggaca tatttagcgc
     2821 ccgttcctta agaccattgt cgtccgggaa tcgttcagcc aaatcaatca tccgcagcgt
     2881 agcctttctg atatacggat acatccagtc atttgcgcta tttaacacat cctctgcata
     2941 gccgccctgc aatccggaag aacagtaggg ctcaatcggc tgtgaaagct gacggggaat
     3001 ggcacgttga caaggaagcg ccgctgatag ttcgggatgt acggcaagtt ggcggaaggt
     3061 ggcctcaagc catgcaggcc cttcgtacca ggattgaccg aataaagacg caggataggt
     3121 tgcacagata cttaccggtg caccttctaa aagagaagat gctttgttga gagtttcaaa
     3181 acgcctctgg ataaactgac gagcgtcttc ttctatctgt gtccgggcaa gcccttcatc
     3241 atataaagag ccctggtcct ggttctccgc agatttagca taatagcgga acccggttat
     3301 tcgccgcccc tgatgcacat caaaaagagg cgaaagcaag gattcactca attcaaaacc
     3361 cacatcgcgc tcggtatcca gatacaccct gttttgatcg aaactactgt ccgtagaggt
     3421 aacatcggtt aaggaaaaaa aatcgcgggc aaacacaagt a
//
LOCUS       DAOQXQ010000100         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28452_length_3460_cov_2.749192, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000100 DAOQXQ010000000
VERSION     DAOQXQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28452_length_3460_cov_2.749192"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PKH81_02415"
     CDS             <1..1312
                     /locus_tag="PKH81_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNQ96921.1"
                     /translation="PAHEGYNVMLTIDNALQYELSKISSETMKETKAEAVMMVVADAK
                     TGEILAYISEPSANLNEYTTSTPSQRVDRPALYAYEPGSVFKIFSTAALLDLGSTADE
                     REYLCDGGYSLESEGRETIHIKCLSKHGRVRPRDVIRLSCNDGMGQMAERASSRDFEA
                     KLRALGFGSKTNIELPGETTGIFASTDQWSIRSKPTIAIGQEISVSALQMVEAATALA
                     NQGELLKLTILSRLYTRDGLLVYEHKRTSLGKVLDSTSAQLVLSYMQSTSESGTGTKA
                     SVGDVPMAVKTGTAQMMNASGGGYSDKDFISSCIGIFPAHDPQIIVYLAIIKPVGETY
                     GGRIAAPVISRAANIIIDSLGMGRAQATSVKHTGLVAVPKSEKVLLGSTMPDLTGVPK
                     RLLSPLFSRTDISIKISGNGYVVEQFPPPGTPVEKGMIIELRLE"
     gene            1293..3131
                     /locus_tag="PKH81_02420"
     CDS             1293..3131
                     /locus_tag="PKH81_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNQ96922.1"
                     /translation="MNYASSDFFISNCRLFSRRFPDQAALLTLDNPDKALKLLALIPP
                     TMNLSPASSGDPTVSVQGQFLHSQRDPRREAEKTLAKSDIFTSDGCLFCGSGLLYLPE
                     LYSSAFPQAPLVIIESELPLFLLALSSRPLDALFAHPQLVIALALPAHEAAALPEQLG
                     FPEIPLFTSPALTAVNPSWWREFEVLVRRNREKRAINANTLKKFGPLWLRNMCKNLQE
                     LGSLGGVQTLEGLARDIPVLLLAAGPSLDTIRPSLHAYSKHCLILAVDTACRVCIQSG
                     VTPDIVVLVDPQYWNYKHLEGLDLSSSILITEGAAWPAVFRITARRRYLCSSLFPLGK
                     FLEKDTIARGELGAGGSVSTTAWDAARLMGAKDIYVAGLDLGYPGKKTHASGSLFEEL
                     SHMHSNRLSPAETTLSAALSSTGLVPAQNWKGQTIATDKRLLLYAWWFESRFASFPEL
                     TTRILGEEGVLIPGAYSARADDILALPVKRDDITRRIQALPPEEDRLVAGFPEAVQRL
                     LDSLEDILLLARDAERLSKRTWTGANELHHITKRLDAIDQKIRTHPAKEVAAMVFSAQ
                     ETEGPASTNTNPLAASQRVYHALVAAAEKNIELIKKFSKEIEVTVR"
     gene            complement(3238..>3460)
                     /locus_tag="PKH81_02425"
     CDS             complement(3238..>3460)
                     /locus_tag="PKH81_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96923.1"
                     /translation="ITAFVGYVLSRIAERRQSLAPAALSASVDAKKRLKAQFGSKQWI
                     RYLDREKSDMEVVLLGKLADETTRWLLSE"
BASE COUNT          900 a          917 c          837 g          806 t
ORIGIN      
        1 tccagcccat gaaggctaca acgtcatgtt gaccatcgac aatgcccttc aatacgagct
       61 atccaaaatt tcctcggaga ccatgaagga aacaaaggca gaagcggtta tgatggttgt
      121 cgcagacgct aaaaccggcg aaatactggc ctatatcagc gaaccttcgg caaacttaaa
      181 tgaatacacc accagcactc cttcccaacg ggtagaccgc cccgccctct atgcctacga
      241 gccgggatcg gtgtttaaaa tattctccac cgcggccctt ctggaccttg gctccacagc
      301 agacgaacgg gaatacctgt gcgacggagg atactccctt gaaagcgagg gaagagaaac
      361 cattcacatt aaatgccttt ccaaacacgg cagggtacgt cctcgggatg ttatcagact
      421 ctcctgtaac gacggtatgg gacaaatggc cgaacgggca tcatcccgtg attttgaggc
      481 taagcttcgc gcgcttggat tcggatccaa aacaaacata gagcttccgg gagaaacgac
      541 aggcatattt gcttccacag accaatggtc aatcaggtca aagccaacta tagccatagg
      601 acaagaaatt tcggtatccg cattgcaaat ggtagaagcg gccacagccc tggcaaatca
      661 gggagagtta cttaaactga caatcctttc ccggctgtat acccgtgacg gacttttagt
      721 ctatgagcat aagcgaacaa gtctgggaaa ggttctggac agtaccagcg cacagctggt
      781 gttatcctat atgcaaagta cctcagaatc gggaactgga acaaaggcat ctgttgggga
      841 cgttcctatg gccgtaaaaa ccggcaccgc ccagatgatg aacgcctccg gtggtggtta
      901 tagcgataaa gactttattt cgagctgtat aggaatcttc ccggcacatg acccccagat
      961 aatagtatat cttgcgataa ttaagccggt tggggaaacc tatggaggaa gaatcgctgc
     1021 cccggttatc tcgcgtgcgg caaatatcat tatagactcc cttggaatgg gccgtgcgca
     1081 ggcaacatca gtaaagcata ccggtttggt agcagtgccc aaaagcgaaa aggtgttgtt
     1141 gggatcgact atgcccgatt taaccggagt acccaaacgt cttttgtctc cactgttttc
     1201 acgcaccgat atctctataa aaatctcggg caatggctat gtggtggaac agttcccacc
     1261 gccgggaaca ccggtagaaa aaggaatgat cattgaatta cgcctcgagt gattttttta
     1321 tttcaaactg ccgtctgttt tcccggcgct ttccggatca ggcggcactc cttactttgg
     1381 ataaccctga caaggcacta aagcttcttg cacttattcc ccctaccatg aacctttccc
     1441 ctgcttcttc gggtgatcca accgtctcgg tacagggaca atttcttcat tcccaaagag
     1501 atccgcgcag agaagcggaa aaaactctgg ccaaatcgga tatctttacc agcgacggct
     1561 gtcttttctg tggctctggc ttactctatc taccagaatt gtacagtagc gcctttcccc
     1621 aggccccact agtcataata gaaagtgaac ttcccctctt cttgcttgcc ctttcctccc
     1681 ggcctttgga tgccctcttt gcccatccac agctggtgat tgcccttgcc ttgccggccc
     1741 acgaagcggc agcccttcca gaacaactcg gttttccgga gatccccctg tttacctcac
     1801 cagcgcttac cgcagttaat ccttcctggt ggcgcgagtt tgaggtgcta gtacggcgaa
     1861 atcgggaaaa aagagccatt aatgcgaaca cactcaaaaa atttggccca ctctggctca
     1921 gaaatatgtg caaaaacctc caggaattag gctctctggg tggagtgcaa acactggaag
     1981 gccttgcacg ggacataccg gtgttacttt tggcagctgg tccctccctg gacaccatca
     2041 ggccctcctt acacgcgtat tccaaacatt gtctcatcct tgccgtagac accgcatgca
     2101 gggtgtgcat tcaatcggga gttactccgg atattgtggt tttggtggac ccgcagtact
     2161 ggaattataa gcatcttgag ggactggacc tatcttcgag catcctgata accgaaggcg
     2221 ctgcatggcc agctgtgttc cgtattacgg ctcgtcgccg ctacctgtgc tcatccttgt
     2281 tcccgttagg aaaatttttg gaaaaagaca ctatagcccg cggagaactg ggagcgggag
     2341 gttcagtatc caccaccgcc tgggatgcag cacggcttat gggagctaaa gacatctatg
     2401 tggcaggcct tgatttaggc tatccaggaa aaaaaaccca tgcaagcggg agtctgtttg
     2461 aagaactaag ccatatgcac agtaatcgcc tttcaccggc cgaaacaacc ctgagtgcgg
     2521 cactcagttc aaccggacta gtcccggctc aaaactggaa gggccagacc atagcgaccg
     2581 acaagagact actcctgtat gcctggtggt ttgaaagccg atttgcctca tttcccgagc
     2641 ttacaaccag gattctcggc gaagaagggg tcctaattcc cggtgcgtac tcggctcggg
     2701 cggatgatat cctggcacta ccggtaaaac gggatgacat tacacggaga atacaggcct
     2761 tgccaccgga ggaagaccga ctggtagccg gttttcccga ggcagtacaa cgattgcttg
     2821 attcactcga agatattctg ttgttggcaa gggacgctga gcgactatcc aaaagaacct
     2881 ggacaggagc gaatgaactc catcacatta cgaaacgcct tgatgctatc gaccaaaaga
     2941 ttcgaacaca tccggccaaa gaggttgcag caatggtctt ttcggctcaa gaaactgagg
     3001 ggcccgcttc gaccaacacc aatcccctag ccgcttcgca gagggtatat cacgcattgg
     3061 tagctgcggc agaaaaaaac attgaactga tcaagaaatt cagtaaagaa atcgaggtaa
     3121 cggtccgata atctaaatac atcaaggttg caatgccttg acggcgagcc agattgacgt
     3181 ccagtcgccg ttattgggca tacgatcgga ccgtatcatt tgatacggtc ttttttttta
     3241 ttccgacaac aaccaacggg tcgtttcatc cgcaagtttg cccaatagca caacttccat
     3301 gtcagatttt tcgcgatcaa ggtatcgaat ccactgtttg ctgccaaatt gtgcctttaa
     3361 ccgctttttg gcatccaccg aagcactaag ggcggcaggg gcgagggatt gccgccgctc
     3421 ggctatacgg gacaatacat acccgacaaa ggcggttatc
//
LOCUS       DAOQXQ010000101         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28526_length_3454_cov_2.460135, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000101 DAOQXQ010000000
VERSION     DAOQXQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28526_length_3454_cov_2.460135"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKH81_02430"
     CDS             <1..505
                     /locus_tag="PKH81_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96924.1"
                     /translation="INVVMWSWCGQVSGSTEASIADYLSRMAALESLYPGVTFVYMTG
                     HSDGSGSSGNLHLRNQQIRDYCAANNKWLFDFYDIECYDPSGTYYGNRHVTDGCNYDY
                     NNSGSTTETGDPAEPTDGDHNWASDWQDAHPGEWWSSGAAHSKPLNANQKAKAAWQLW
                     CAIARQM"
     gene            complement(596..991)
                     /locus_tag="PKH81_02435"
     CDS             complement(596..991)
                     /locus_tag="PKH81_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96925.1"
                     /translation="MKTIYVYVMDTLADWELGYVTAELKSGRFFKKGAPAVSVKTVSY
                     SRPHTSNGPGFLEMMSPAYTGQAFYKDEPSVSDANLITAGSTAALPWAKHIIEHTGVF
                     SAETLEAWYAYVSTGKPEHFFALINSVHQ"
     gene            1080..2237
                     /locus_tag="PKH81_02440"
     CDS             1080..2237
                     /locus_tag="PKH81_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96926.1"
                     /translation="MKTTTRINRSYPATTWFREQTATFADRWAQASWQENGDKTLMPL
                     KQSIRKYASCINELPEGFLPSVFDQHLAVLSEGYEYQILDIIEDLGDERFLCKNEQHE
                     YFCVWSHSVAINVNEGMTAFLTAIIRLEDTSETVSAITYGPVIAWTSLCADDFYRLAR
                     NIARDLLKLKGLSAVIRRDPVPFWALWTLSGIPRAMHGTEEVCTCWVEGRFTENPETH
                     LSTGWKRDMIGKRVRLRKSGSKPFFEQVVIYDTKTMNAIVLARRNSYLEKLVDSLKTV
                     FVAQADKEGFVTPVVEIALSDVFRIQPEYKKWTSPFDREDQRKEEAKDALNPERKAEL
                     DVFNAAMKDLLPYVNSGTIPDWKKLAATHSLSNESLESLKDMYEQLRKKLQ"
     gene            complement(2275..2559)
                     /locus_tag="PKH81_02445"
     CDS             complement(2275..2559)
                     /locus_tag="PKH81_02445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ96927.1"
                     /translation="MTADLFKAIGHPDRLAMLEKLADKPWCVCDLALDLGLNKSVASK
                     HLSLLHDLGLIELEKKGTQVIYTLCVPCVIDMGKCSYLAVLNERKKRLGS"
     gene            2683..2910
                     /locus_tag="PKH81_02450"
     CDS             2683..2910
                     /locus_tag="PKH81_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ96928.1"
                     /translation="MKIQILGTGCPKCKQLEAHAREAVAIKGVTAEIEKVTDIDQIME
                     MGVMMTPALVIDGEVRSVGKLLTVDQIAAQL"
     gene            2922..>3454
                     /locus_tag="PKH81_02455"
     CDS             2922..>3454
                     /locus_tag="PKH81_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ96929.1"
                     /translation="MAKKVYSPNVIVLALALVFVAIYFIPWDAPRVTTAILEGFQMLG
                     EYAREHVLLCLVPAMFIAGAIGVFLNQQAVMKYLGPKAPKTVAYAVASVSGAILAVCS
                     CTVLPLFKGIYKKGAGIGPAISFLYSGPAISIIAITMSFQVFGWKIGLARTIGAITFA
                     IVIGLCMQMIFRKEDEAR"
BASE COUNT          887 a          752 c          892 g          923 t
ORIGIN      
        1 gataaacgtc gttatgtggt cctggtgtgg gcaggtttcc ggatcgaccg aggcaagcat
       61 cgcagattat ttgagcagga tggccgccct ggagagtctc taccccggag taacctttgt
      121 gtacatgacc ggacattcgg acggtagcgg ctcctccggt aatctacatc tgcgaaatca
      181 acaaatccgc gactattgtg ccgccaataa taagtggctg ttcgatttct atgatattga
      241 atgctacgat ccgagcggaa cctactacgg caatcgacac gtcaccgacg gctgtaatta
      301 tgactataac aatagtggca gcaccacaga gaccggcgat cccgccgagc cgactgacgg
      361 cgaccataat tgggcaagcg attggcagga tgcccatccc ggcgagtggt ggagtagtgg
      421 cgcggcgcac tccaagcccc tgaacgcgaa ccagaaagct aaggccgcct ggcagctgtg
      481 gtgtgcgata gctcgtcaga tgtaaacagg ccttcgcccc tgctacgact ttcgcacgcg
      541 gcggctccgc gcgaggattg tcgccttagt gtttctttct gcctcaatta cctggttatt
      601 gatgaaccga atttatgagc gcaaaaaagt gttcgggttt gcccgtgcta acatacgcgt
      661 accaggcttc cagagtctcg gccgagaaaa caccggtatg ctcgatgatg tgctttgccc
      721 agggtaatgc tgcggtagag ccggcggtaa tcaggtttgc atcagacaca gacggctcgt
      781 ctttatagaa ggcctgtccg gtgtaggcgg gagacatcat ttccagaaat cccggaccgt
      841 tactggtatg agggcggctg taactgactg ttttgaccga tacagcgggc gctccttttt
      901 tgaaaaacct gccagatttg agctccgcgg taacataccc caactcccaa tccgccaggg
      961 tatccataac atacacgtag atagtcttca tataatggct ccttatgagg aagagtatac
     1021 atagttaatg ctgacaacag tatggcagtg tttatgggtt atatggtaaa gtgtaccgta
     1081 tgaaaactac cacccggatt aatagatcat atccagctac tacctggttt cgcgaacaaa
     1141 ctgctacctt tgccgatcga tgggcacagg caagttggca agaaaacggg gataaaaccc
     1201 ttatgcccct taagcagtca atacgcaaat atgcatcctg tatcaatgaa ttgccggaag
     1261 gctttcttcc ttctgttttc gatcaacatc ttgcagttct ttccgaggga tatgagtatc
     1321 agattctgga tattatcgaa gaccttggcg atgagcgatt cctttgcaaa aatgaacaac
     1381 acgagtattt ttgcgtgtgg tctcattctg tggcaatcaa tgttaatgaa ggaatgacgg
     1441 cctttcttac ggcaattata cggctagagg atactagtga gacggtttct gccattacct
     1501 atggccccgt cattgcatgg acctcgctct gtgccgatga cttttatcgc cttgcgcgaa
     1561 acatagctcg tgatttactc aagttaaaag gtctgagtgc ggtaataaga cgagatcctg
     1621 ttccgttttg ggctctatgg actctctcgg gcattcctcg agccatgcat ggaacggaag
     1681 aagtttgtac ttgttgggtc gaaggccgat tcacggaaaa tccggagact catctttcaa
     1741 ctggatggaa gcgtgacatg attggtaaac gggtacgcct tcggaaaagt ggctcaaaac
     1801 ctttttttga acaggtggtc atttacgaca caaagaccat gaacgcgatt gttcttgctc
     1861 gtcgaaatag ctatcttgaa aaattggtag actctcttaa gacagtgttt gtcgcacagg
     1921 ccgataaaga gggctttgta acgccggtgg tcgaaatagc cctttcggat gtgttccgga
     1981 ttcaacctga atataaaaaa tggacgagcc cttttgatcg tgaggatcaa cggaaagaag
     2041 aggcaaaaga tgcccttaat ccggaacgga aggcggagct tgatgttttt aacgctgcca
     2101 tgaaggattt acttccgtat gtgaactcgg gaacaatacc cgattggaaa aagcttgcag
     2161 caacgcattc gctttctaac gagagtcttg aatcactaaa ggatatgtat gagcaattgc
     2221 gaaagaagct tcagtaaaat ttcataactg ctcacaggat tgttggtatt ttccttaaga
     2281 gccgagtctt tttttgcgct cattaagcac cgcaagatag ctgcactttc ccatatcgat
     2341 tacgcagggt acacagagtg tgtagataac ctgggttccc ttcttttcca gttctatcag
     2401 gccaagatca tggagaaggg acaggtgttt tgacgcaact gacttgttta atccaaggtc
     2461 cagggcaagg tcgcagacgc accaaggctt atcggcgagc ttttcgagca tggcaagtcg
     2521 gtcagggtga ccgattgcct taaataagtc agcggtcatt tgtagtcttc ttttttccga
     2581 ttccttcatg ctgttattgt tgcaatgtta ggaaactttg tcaataaacc cttgacaatt
     2641 agtgcaaagt ttccaataat tgaaacacaa ggagtgttac cgatgaagat tcagattctg
     2701 gggactggtt gccccaaatg caagcaatta gaggcacatg cccgtgaagc ggtagcgatt
     2761 aagggcgtta ccgcagaaat agaaaaagtt accgacatag atcagattat ggagatggga
     2821 gtaatgatga ctcccgccct tgtgatagat ggagaggttc gaagcgtggg gaaactgcta
     2881 acagtagatc agatagcagc tcaattgtaa ggtggatatc catggcaaag aaagtctata
     2941 gcccgaatgt gatcgtgctt gcccttgcgc ttgtgtttgt agcgatttat tttattccct
     3001 gggatgcccc tcgtgtaacg acagcaattc tggaaggatt ccagatgctt ggtgagtatg
     3061 cacgggaaca tgttctactg tgtcttgtcc ctgctatgtt tattgccggt gcgattggag
     3121 tatttcttaa tcaacaagcg gtgatgaagt atcttggtcc aaaggcgcca aagacggtag
     3181 cttatgcggt tgcatcggta tctggggcga ttttagcagt gtgttcctgt accgtacttc
     3241 cgctttttaa ggggatatac aaaaagggtg cgggaatcgg gcctgcgatc agttttttat
     3301 attcagggcc ggcaattagc attattgcga ttacgatgtc gtttcaggta tttggatgga
     3361 agattggatt ggcgcgtacg attggcgcaa ttacttttgc aatagtcatt gggctttgta
     3421 tgcagatgat ctttcgaaaa gaagatgagg cccg
//
LOCUS       DAOQXQ010000102         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28781_length_3432_cov_2.709505, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000102 DAOQXQ010000000
VERSION     DAOQXQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28781_length_3432_cov_2.709505"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKH81_02460"
     CDS             <1..1006
                     /locus_tag="PKH81_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ96930.1"
                     /translation="VGRNPSGMYKAILQVLTGYNIDRGRFYVRYVGEWLAQSMPFILC
                     GLSMGFAARTGLFNIGGEGQYVVGLTVAQFVAIMGPAIPTVHWLLALILAMTAGALWG
                     GIVGFLKARFEVSEVVATIMLNYIALYASRILVMSIPGTNTYKTQDYPITALLRSSIL
                     ERITKGSLLNLGIIFVILSVCVYWFIIEKTRLGFALRATGLNKDAARASGIPVVKSII
                     LSMAIAGAFAGLAGGIVSLGSFKFGRVLAGNDGYGFAGIAVALVGNSTALGTMLAGLL
                     FGMLSSAQPLMESNQIPKEITFIIQGLVVVFIAIRTGFRMFIRYSMKKNQLKESLVDE
                     "
     gene            999..1904
                     /locus_tag="PKH81_02465"
     CDS             999..1904
                     /locus_tag="PKH81_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ96931.1"
                     /translation="MNNIFGKIPTILMLVSPILITATGGMICERSGIVNIALEGLMVF
                     GAFAAATAHFFLEPVLGPSSVWVSLSIGAIAGVLFSLIHAFASVSLNADQTVSGTGIN
                     LLANGVTIFLAQIFFKQDRTKQFMLGMMPGQWGVYPTAYIALTVVFLSWLLLYRRRFG
                     LRLRACGENPQAAASVGINVLAIRYIAVLLSGLLAGLAGGCLVLTTNTQFNSTTVNGH
                     GFIALAVVSFGRWRPQGIAASSILFGTALAFSIIANDFTALKSLPTEFFNIMPYAITL
                     LTLVLFSGKNYAPLSAGKPYEKGSS"
     gene            1907..2539
                     /locus_tag="PKH81_02470"
     CDS             1907..2539
                     /locus_tag="PKH81_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ96932.1"
                     /translation="MKDTRDSFLSVMKRRYACKLYDTTHSLSRDTLAFIMEAGRLSPS
                     SFGLEHWHFWVLTSDNSLSLISEACFSQDAVATSPAVVCIMCRQAHVYAPSSPFVAER
                     ASRFPGGLLVFLADYSSYYAYLETEGITNQWARSQCYIAAANLMSAAASCDVDSCPIE
                     GFDNRKVLSTLSLDPNIWQTGLIVSLGYGKPDEADRLRIRMNSEDIVTYV"
     gene            2532..>3432
                     /locus_tag="PKH81_02475"
     CDS             2532..>3432
                     /locus_tag="PKH81_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ96933.1"
                     /translation="MFNQSFVLKIFEGFSIERWNDLVRPFEMIEMDKTAEKAVLAYII
                     GKFEEKRGNTIDWNRVIYGTFFDLLRKIALCDIKAPVQRMIRDDYPDEYERINKWAIE
                     QYRSLIDDPALMTAFEEHVLSPSDPSDITWRVLRAAHKYSALREVEMLRMVNEPFRLV
                     PIEKGLNKDIEGFLDLRGMQLLFGRQQPYDFLMMIERLRFQTRWNQTPRIPRTSVLGH
                     CYFVAVLTLLLSREIGANDGRVYNNFFSALFHDLPEAVTRDIISPVKQATDHLPSIVK
                     EIEDAIVKTELLPLMDDFFRDELC"
BASE COUNT          834 a          695 c          767 g         1136 t
ORIGIN      
        1 tgttggcaga aatccctctg gcatgtataa agcgattctt caggttctca ctggatacaa
       61 catagatcgt ggaagattct atgtccgata tgtgggtgaa tggcttgctc aatctatgcc
      121 gtttatcctt tgtggtcttt ctatgggatt tgccgctcgt accggtcttt ttaatattgg
      181 tggtgaaggc cagtatgttg taggattaac cgtagcgcag tttgtcgcta ttatgggtcc
      241 tgccattcct accgtgcatt ggcttttagc ccttatcctc gcgatgaccg ccggagcttt
      301 gtggggtggt attgtaggct ttcttaaagc ccgcttcgag gtatccgagg tggttgccac
      361 aattatgctc aattacatag ctctttatgc ctcgcgtatt cttgttatgt ccattcctgg
      421 tacaaatacc tataagactc aagattatcc gataacggct ctcttgcgat cttcgatttt
      481 ggaacgcata accaaggggt ccttattaaa tctgggcatt atttttgtta tcctttcagt
      541 gtgtgtgtat tggttcatta tagaaaaaac acggttagga tttgccttga gggcaacagg
      601 acttaataaa gatgcagcaa gagcaagtgg tatacctgtg gttaaaagta tcattctgtc
      661 aatggcaatc gccggagctt ttgcggggct tgccggagga atcgtttctt tgggttcttt
      721 taaatttgga cgggttcttg ccgggaatga tgggtatggt tttgccggta ttgccgttgc
      781 gcttgtaggc aacagcactg ccttgggtac aatgctcgca gggcttttat ttggaatgct
      841 ttcctctgcc cagccattga tggaatcaaa ccaaatacca aaggaaatca cctttattat
      901 acaaggtctt gtagttgtct ttattgcgat acgaactggc ttccgtatgt ttattcgcta
      961 ttcaatgaaa aaaaatcagc taaaggagag cctcgtcgat gaataatatc tttggtaaaa
     1021 taccaactat cctgatgctt gtttctccaa ttctgataac agctactggt ggtatgattt
     1081 gcgagcgctc cggtatcgta aatatcgcgc tggaaggact aatggtattt ggtgcctttg
     1141 ctgctgctac cgctcacttt tttcttgaac ccgtgctcgg tccatcctct gtatgggttt
     1201 ctctaagcat tggcgcaatc gctggtgttt tgttttctct tattcatgca tttgcttcag
     1261 tgtctcttaa tgccgatcaa acggtttctg gcacaggtat taacctttta gcaaacgggg
     1321 ttactatttt tttggcgcaa atctttttta agcaggatcg caccaagcaa ttcatgcttg
     1381 ggatgatgcc cggacaatgg ggtgtgtatc ccaccgcata tatagcatta acagttgtgt
     1441 ttctttcatg gttattgctt tatcgtagac gatttggact tcgtttgcgt gcttgcggag
     1501 aaaatccaca agcagcagcc tcggtaggaa ttaacgtatt agctattcgc tatatagcgg
     1561 ttcttttatc ggggttatta gccggccttg ccggaggttg tcttgtgtta accacaaaca
     1621 cacaatttaa ctcaactaca gttaacggcc atggatttat cgctctggca gttgtttctt
     1681 ttggcaggtg gagacctcag ggtattgcag cctcttccat tctttttgga acagccttgg
     1741 ccttttcgat tatagccaat gattttaccg cgcttaaaag tttaccaacc gagtttttta
     1801 atattatgcc atatgccata accctgttaa cgctggtgct gtttagcgga aaaaattatg
     1861 ctcctttatc tgccggaaaa ccgtatgaaa aagggtcaag ctgaacatga aagacacacg
     1921 agattctttt ctctctgtta tgaagcggcg atatgcgtgc aaattatatg atacaactca
     1981 ttctctttct cgtgatactc ttgcctttat aatggaagcg ggtcgtcttt caccttcttc
     2041 ttttggactt gaacattggc atttttgggt gctcacatca gacaactctt tatccttgat
     2101 ttctgaagct tgttttagtc aggatgcagt tgcaacgtcg cctgctgttg tctgtataat
     2161 gtgtcgccag gctcatgtgt atgccccctc ttctcctttc gttgccgaac gagcctctcg
     2221 ttttccggga ggcttactgg tttttttagc tgattactca tcgtactatg cttacttaga
     2281 gaccgaagga attacaaatc aatgggctcg ctcacaatgt tatattgccg ctgctaatct
     2341 catgtcagcg gctgcatctt gcgatgtaga ttcttgccct attgaaggct ttgataaccg
     2401 caaagttctt tctactttat ctcttgatcc caatatctgg caaacgggtt tgatcgtttc
     2461 gcttggctat ggaaagcctg atgaagctga tcgacttcgg attagaatga acagcgagga
     2521 tattgtcacc tatgtttaac caatcatttg tattaaaaat atttgaagga ttttctatag
     2581 agcggtggaa cgaccttgtt cgtcctttcg aaatgataga gatggacaaa actgcagaaa
     2641 aagctgttct tgcctatatc atcggaaagt ttgaagagaa acgaggaaat accatagatt
     2701 ggaatcgggt tatctatggt actttttttg atttgttacg caagatcgct ctttgtgata
     2761 tcaaggctcc agtacagcgg atgattcggg atgattatcc ggatgaatat gagcggatta
     2821 acaaatgggc gatagagcaa taccgaagcc tcattgatga tccagcatta atgactgcgt
     2881 ttgaagaaca tgtattatct ccttccgatc catcggatat tacctggcgt gttctaaggg
     2941 ccgctcacaa gtattcagca ttacgagaag tagaaatgtt acgaatggta aatgaaccgt
     3001 tccgtcttgt tccgatcgaa aaaggcctta acaaggatat tgagggattc ttggatcttc
     3061 gagggatgca gcttttattt ggtcgacagc aaccatatga ttttttaatg atgattgaac
     3121 gtttgcggtt tcaaacccga tggaatcaaa cccctcgaat accacgtaca agcgtacttg
     3181 ggcattgcta ttttgttgca gttcttactc tattattaag cagagaaatt ggagccaatg
     3241 atggtcgggt gtataataat ttcttttccg cgttgtttca tgatttaccc gaagcggtta
     3301 cccgagatat tatttctcct gtaaagcaag caactgatca tcttccctca attgttaaag
     3361 aaatcgaaga tgctattgtc aaaaccgaat tacttccact tatggatgat ttttttcggg
     3421 atgaattatg tt
//
LOCUS       DAOQXQ010000103         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28904_length_3424_cov_2.279311, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000103 DAOQXQ010000000
VERSION     DAOQXQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28904_length_3424_cov_2.279311"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="lepB"
                     /locus_tag="PKH81_02480"
     CDS             complement(<1..474)
                     /gene="lepB"
                     /locus_tag="PKH81_02480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ96934.1"
                     /translation="MKTPMKPASVFLFVLILSILIKLCVLDIMRVSGPSMLPTLTEGT
                     IVCEYKLAWGIPVPFRNSYFLRWGEPKAGDLIIYPWLGRYVIKRCLATGGTKLVFSND
                     SGYSVQMGEVMLPLTREHYEKMRHITEVPVGTVFAAGDNRAESRDSREYGCISLDS"
     gene            523..1629
                     /locus_tag="PKH81_02485"
     CDS             523..1629
                     /locus_tag="PKH81_02485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ96935.1"
                     /translation="MDVISVVQPENNVVRKKILHGSGFTPIKPPFFGEISSASVSSRS
                     MSLSALGSWPVISSFPFLFCALAAMLVLVAPVIQDRVGLYRLGSIDLPVDSGLELSFK
                     PEIYRTDPADHAAIATDESIPLSIQPVSYSDYRVRPGDSVSRILSRTNLQNIGTILAV
                     NNIQNARRIHAGQVLRIPSMDGIIHTVIRGESLERIAKRYSLTVTALLDANDLASETL
                     TKGQALFIPGASLKIHDLRRAMGELFVMPVRGRLTSRYGYRKDPFTGVRSFHTGIDLA
                     APTGTSVKATLDGRVATTGYSPVYGNYIILSHDGGYQSLYGHLSAISTRRGQYIQQGI
                     VIGKVGNTGYSTGSHLHFSVYKGGKTVDPQTLLK"
     gene            1640..1906
                     /locus_tag="PKH81_02490"
     CDS             1640..1906
                     /locus_tag="PKH81_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96936.1"
                     /translation="MKDKGSKMDGTSICHACGRTMESVFSYCPWCGQATEQTLSLEHQ
                     FEEVFSRVETVRNGNNTVRMERIETALGNLERELSLMLSGTKNE"
     gene            1919..>3424
                     /locus_tag="PKH81_02495"
     CDS             1919..>3424
                     /locus_tag="PKH81_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96937.1"
                     /translation="MKSIVCRTGFALICMSLCAQITFQDPDINAGNEVLFTISANIPG
                     GQQYATLFLRNVESGAMEQLTFYPESIQSLSSGKILQLRNRFGTARFDASSETFSWID
                     EGRPFVRGGPILKGLLPDVAASPDGKWLVLVEPVTSARGRLVLYNADKSVRYVLADSI
                     ERGSIPVSWAPDSSVMVYALGGTLYFARPENFFSVSTVGQQFRIVGPGEISCISWFGP
                     NRFLYARGRNVYRIQTPELFARSLYSSLIGVGELAGKLAVDFDPLSDRFSASPEGDAV
                     IHARGNRTVQYFPLAGDDFARVSNQSQLPWLLLPGNTAWIMPVWTQAGVPLVFASSVY
                     DGSSVIRGWRLADVTGSRIFSPLPMPEGAVSLEVSRTGSHIAFISKDALRIYSSSQWA
                     EIALFREEPVVSIAWADEGRLFIGGTQTVRRWNFLTNTSDFFLLSSISRSGWDEKLSS
                     VVCETVSSEKRTYTGNMKWLLTPSARMRPAQAVNTRWRLYLDSGKGFYDNML"
BASE COUNT          799 a          809 c          855 g          961 t
ORIGIN      
        1 gctgtccagg gagatacatc catattcacg cgaatcgcgg gactcagcgc gattatcacc
       61 cgcggcaaag acggtaccga caggaacttc cgttatatga cgcatttttt catagtgttc
      121 cctggttagc ggtagcataa cctctcccat ttgaacacta tatcctgaat cgttcgaaaa
      181 taccagcttg gttccgccgg tggccagaca gcgttttata acatatcgcc ctaaccaggg
      241 gtaaataatc aagtctcctg ccttgggttc tccccaacgt aaaaaataac tattcctgaa
      301 aggtaccggt attccccagg cgagtttgta ttcacataca atggttccct cggttaaggt
      361 tggtaacatg gatggaccgg agacccgcat aatatccaaa acgcacaatt tgatcaggat
      421 tgagaggatt aacacaaaaa gaaacacaga tgcaggtttc attggcgttt tcatggtgat
      481 tccattctaa ttatccagtg ctgttgtgtc gatacttttt acatggatgt aatcagtgtt
      541 gttcagccag aaaataacgt tgttagaaaa aagatactgc atggttcggg ctttactccc
      601 ataaagcctc ctttttttgg tgaaatctcg tctgcgagtg tttcctcccg ctctatgtcg
      661 ttgtctgccc ttggctcatg gccagttatc tcttcgttcc cctttctttt ttgcgccttg
      721 gcagccatgc ttgtgcttgt cgcgccggtt attcaggatc gagtagggct ctatcgcctg
      781 gggagcatcg acctcccggt ggatagcggc ttggaactct cttttaaacc agagatatat
      841 cgcaccgatc cagccgatca tgcagcgata gccacggatg agtctatccc gctatccatt
      901 caaccagtga gctacagcga ctatcgggtg cgccctggcg attcagtgag tcgaatcctt
      961 tcacggacta atttgcagaa tattggcacc atcctggctg ttaataatat tcaaaatgct
     1021 cgaagaatac acgcaggtca ggtccttcgt attccctcaa tggatggaat tattcatacc
     1081 gttattcgcg gagaatctct tgagcgaata gccaaacgct attcccttac ggtaaccgcg
     1141 cttctggatg caaatgattt ggccagcgaa acccttacca agggacaagc cctttttatt
     1201 cccggtgctt cgctcaagat acatgatctg agacgagcga tgggtgaatt gtttgtaatg
     1261 cccgtacggg gtcgacttac ctctcgctat gggtatagaa aggatccttt taccggggtg
     1321 cgaagttttc atacgggaat tgatctagcg gccccaacgg gaacctctgt taaggccacc
     1381 cttgatggca gggttgcaac caccggctat tcgccagtat acggaaatta tattatcctt
     1441 agtcatgacg gaggatatca atccttgtat ggtcatttga gcgcgatttc tacccgccgc
     1501 ggtcagtata ttcagcaggg aattgttatc ggtaaggttg gaaataccgg ctattctacc
     1561 ggaagccatc tacacttttc tgtctataag ggcggtaaga ccgtagatcc gcaaaccctg
     1621 cttaaatagg tgtgctataa tgaaagataa aggaagcaag atggacggga cttcgatttg
     1681 tcatgcctgt ggaagaacca tggaaagtgt tttctcctat tgtccctggt gtggacaggc
     1741 aactgaacaa accctgtcgc tggaacacca gtttgaagag gttttttcac gtgtcgaaac
     1801 cgtgcgaaat ggcaataata cggtgcggat ggaacgtata gaaactgcgc tgggaaatct
     1861 tgagcgggaa ctgtccctga tgctttccgg aactaaaaac gaataacagg aagagaccat
     1921 gaaatcgatc gtatgccgga caggatttgc cctgatatgt atgtcgcttt gcgcacagat
     1981 aacgtttcag gatcccgata ttaacgccgg aaacgaggtg ctttttacta tatctgcgaa
     2041 tattcccggc ggacagcagt atgctacttt gtttttacgc aatgttgaat ccggggcaat
     2101 ggagcaactc accttttatc ctgaatccat ccagtctctt tcatcgggta aaatactgca
     2161 actgcgcaat cgttttggaa cagctcgttt tgacgcatcc agcgaaactt tttcatggat
     2221 cgacgagggg cgtccttttg tccgcggggg tccaatacta aaaggcttgc tcccggatgt
     2281 tgcggcaagc cccgacggta aatggcttgt tttggtcgag ccggtaacat ctgcccgggg
     2341 tcgccttgta ttatacaatg cggataaatc ggtgcggtat gtcctggctg attcgataga
     2401 acggggaagt attcccgttt cctgggcacc tgattcctcg gtgatggtgt atgcccttgg
     2461 gggcactctg tattttgccc gtccagaaaa cttcttttcg gtatctactg ttggacagca
     2521 atttagaata gtagggccag gtgaaatttc ctgcataagc tggttcggtc ccaatcgttt
     2581 tttatatgct cgtgggcgca atgtctatcg tattcaaacg cctgaacttt ttgcacgttc
     2641 tctgtacagc tccctgattg gagtggggga gcttgccggt aaactcgcgg tggattttga
     2701 cccgctttcc gatcgttttt ctgcgtctcc agaaggggac gcggttatcc atgcccgcgg
     2761 gaatcgaacc gttcagtatt ttcctcttgc cggggacgat tttgcccgag tgtccaatca
     2821 gtctcagctt ccgtggcttt tgcttccggg gaataccgca tggataatgc cggtgtggac
     2881 ccaggctggc gtacccctgg tgtttgcgtc gtcggtatat gatggctcct ctgtaattcg
     2941 cggatggaga cttgctgatg taactggctc ccgcattttt tctccactac ccatgccaga
     3001 gggagccgtt tcccttgagg tatcacgcac cgggtcacac atagctttta tcagtaagga
     3061 tgcactcagg atttatagca gttcgcaatg ggccgaaatc gccttgtttc gcgaagagcc
     3121 ggtggtaagc attgcttggg ccgatgaggg tcgactattt atcggtggca ctcagacggt
     3181 acgtcggtgg aactttctaa ccaatacttc tgacttcttt ttgctctctt ctatttcacg
     3241 ttcgggctgg gatgaaaaac tctcctccgt tgtctgcgaa acggtgtctt cggaaaaaag
     3301 aacgtatacc ggtaacatga aatggttgtt gacgccttct gcaaggatgc gacctgcaca
     3361 ggcggtaaac actcggtgga gattgtatct ggatagtggc aagggatttt atgataacat
     3421 gttg
//
LOCUS       DAOQXQ010000104         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_28932_length_3421_cov_3.065359, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000104 DAOQXQ010000000
VERSION     DAOQXQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28932_length_3421_cov_3.065359"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PKH81_02500"
     CDS             <1..1164
                     /locus_tag="PKH81_02500"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNQ96938.1"
                     /translation="GEHYQAELPAVPNEYSVEKKAQDAHEAIRPTYVTYTPEYVKEHL
                     TRDQLRLYSIIWERFVSSQMSNARSRTSSIDIASGNALFRVSSTRVFEKGFYKVIKTL
                     VSKDEKEKTLPEMKIGEELTVKEFHPEQHFTQGPARFTDASIVKTLEEKGIGRPSTYA
                     PIISVLLDRYYVTRSNKQLVPTQLGRIINDLLVGYFPAVINVDFTANVETELDQVEEG
                     STQWPSMIGSFYQPFKSRVDEVMETLDSMKGRLDETTDHVCELCGKPMLKKLGRFGFF
                     LACSGFPECRNTRSIPLAKCPRPGCTGEIVSRRTKGRGKEFFGCTNYPECSFISHFKP
                     INANCPKCGQFLVEKYDKKNGSFKSCINPDCDYLHTQEEVIPVLSDSEAEGDE"
     gene            1161..2057
                     /locus_tag="PKH81_02505"
     CDS             1161..2057
                     /locus_tag="PKH81_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ96939.1"
                     /translation="MNRYCEEYLEYLVGVRSLSPRSIASYRRDLHLFFTIRPVDPLSA
                     SANDVRLFLSELSRKGYEKTSINRMLAALRGFFRYLVRFSLRKDNPALTLRNLKTVKT
                     VPLFLFAPEAEQLCALPLSESTKIKPLWATRDSALFSALYSSGCRVSEIAGITLSSLS
                     SAVNSAIVRGKGNKERTVFFSKTAQTLLREYLLERSEILSRTQETVCNSLFISKRGKP
                     LSVRGIQFILSHYTDGTGDLIHLSPHALRHSFATTLISRGADLRVVQEMLGHETISTT
                     QRYTHITSERLERLYHQAHPHG"
     gene            2071..2610
                     /gene="hslV"
                     /locus_tag="PKH81_02510"
     CDS             2071..2610
                     /gene="hslV"
                     /locus_tag="PKH81_02510"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNQ96940.1"
                     /translation="MNQKKIRSTTIIAVRRNGKIAMAGDGQVTMGETIMKPNARKVRR
                     IYDGNILTGFAGATADAFTLLEKFEMRVKEYAGDLTRSAVELAKDWRTDKALRNLQAL
                     LLVADKEKTLLISGTGDVIEPADAAIAIGSGGNYAYAAALAYLDSTDLPALEIARKSM
                     EIAAGICIYTNDHILVEEL"
     gene            2613..>3421
                     /locus_tag="PKH81_02515"
     CDS             2613..>3421
                     /locus_tag="PKH81_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96941.1"
                     /translation="MMDGLESLTPKAIVAELDKYIIGQNKAKRAVAIALRNRTRRLKL
                     PQEVRDEVAPKNILMIGPTGVGKTEIARRLAKLCGAPFLKVEATKYTEVGYVGRDVES
                     MIRDLMATGFSMVKSEMQSTLRGEAEKRAEEVLLDLLLPGIKKKTEAYKTTHEHGLII
                     EDGSVEKSPVPPVVPAASTESVDTREKFRLMLREGKLDERVVEITVQRGGMPNLEVFA
                     GSANLEDLEMSVANITNMITGNRNKRKQVTVKDAREIIIAEQLDKIVDQDK"
BASE COUNT         1003 a          690 c          792 g          936 t
ORIGIN      
        1 ggagagcatt atcaagctga gcttccagcg gttcctaatg agtactctgt tgaaaaaaag
       61 gcacaagatg cacacgaagc tatccgtcct acgtacgtta cctatacccc ggaatatgta
      121 aaggaacacc tcactcgcga tcagctcagg ttgtacagta ttatttggga gcggtttgta
      181 tcaagtcaaa tgtcaaacgc gcgaagcaga accagcagca ttgatatcgc ttcaggaaat
      241 gcccttttta gagtatcttc aacccgtgtt tttgagaaag gtttttataa ggtcataaaa
      301 accctcgttt caaaggatga aaaagaaaaa accttaccag agatgaaaat tggcgaggaa
      361 ttgactgtaa aagaatttca tcctgaacaa cattttaccc aaggaccggc acgctttacc
      421 gatgcctcta ttgtaaaaac actggaagaa aagggtattg gtcgcccttc aacctatgca
      481 ccaattattt cggttcttct ggatcgttat tatgtcactc gttcaaacaa acagttagtt
      541 ccaactcaac ttgggcgaat cataaacgac ctgcttgttg gatatttccc ggcggtaata
      601 aatgtggatt ttaccgccaa cgtagaaact gaattggatc aagttgaaga aggttctact
      661 caatggcctt cgatgattgg atctttttac cagccattta aaagtcgagt agacgaggtt
      721 atggaaactc tggacagtat gaaaggtcgt ctggatgaga caaccgatca tgtatgtgag
      781 ttatgcggga aaccaatgct aaaaaaactt ggacgctttg gcttctttct tgcctgttcc
      841 ggttttccag aatgccgcaa tacacgatcc ataccccttg ctaaatgccc acggccaggg
      901 tgcactggag aaattgtgtc acgaaggaca aaagggcggg gaaaggaatt ctttgggtgt
      961 acaaattatc cggaatgcag ctttatttcg cactttaaac ccataaatgc aaattgtcct
     1021 aagtgtgggc aatttctggt ggagaagtac gataaaaaga atggttcgtt taaaagttgc
     1081 atcaatcctg attgtgatta tttacatacc caagaagaag taatacctgt actttcggac
     1141 agtgaagcgg aaggggatga atgaaccggt actgcgaaga gtatcttgag tatttggtag
     1201 gggttcgaag cctttctccc cgttcaattg ccagttatcg aagagattta caccttttct
     1261 ttacaataag gcccgttgat cctctatctg catcggctaa tgacgtacgc ctttttcttt
     1321 cggaattaag tcgcaaggga tacgaaaaaa caagtattaa tcgtatgtta gcggctttgc
     1381 gcggtttttt tcgttatctg gttcgattct cgcttcgtaa ggataatccc gcacttacac
     1441 tcagaaacct taaaacagtc aaaacggttc cgcttttttt atttgcgcca gaagctgaac
     1501 agttatgcgc ccttccgctc agtgagtcaa caaaaatcaa accgttatgg gcaacccgcg
     1561 attctgcctt gttttcggcc ctgtattcct ccggctgtcg tgtttccgag atcgcaggga
     1621 ttaccctttc ttctttatcg tctgcagtca attctgcaat tgtacgagga aaaggtaaca
     1681 aggaacgtac tgttttcttt tccaaaacag cacaaacatt acttcgcgaa tacttgcttg
     1741 agcgatctga gatcctctct cgtactcagg aaactgtgtg taatagtctt tttatttcaa
     1801 agcgtggcaa gccattgtcg gttcgaggta ttcagtttat tctgtctcat tatacagatg
     1861 gaaccggaga tcttatccat ctatctccac atgcccttag gcatagtttt gctactacat
     1921 tgattagccg gggagctgat ctgagagtcg tacaagagat gttgggacat gagactattt
     1981 caacaacaca gcgatacaca catataacaa gcgaacgact ggagcgtctg tatcatcagg
     2041 cgcatccaca tgggtaaata aggagttatg atgaatcaaa aaaaaattag aagtactact
     2101 attatcgctg ttaggcgaaa tggaaaaata gccatggccg gagatggtca agtaaccatg
     2161 ggtgaaacaa ttatgaaacc taatgctcgt aaggtacgta gaatttacga cggtaacata
     2221 cttactggct tcgccggagc aactgccgac gcttttaccc tgcttgaaaa attcgagatg
     2281 cgagtaaagg agtatgccgg agacttaacc cgctcagccg ttgaacttgc caaagattgg
     2341 cggacggaca aggcattacg taatttacag gcattgttat tagttgcgga caaggagaaa
     2401 actcttttaa tctctggtac cggagacgtg attgaacctg cggatgctgc tattgcaata
     2461 ggatcgggcg gtaattatgc atatgcggct gctttggcct atcttgattc aaccgatttg
     2521 cctgctctcg agattgcccg taaaagcatg gagattgcag caggtatttg tatttatact
     2581 aatgatcata tccttgtgga ggagttataa acataatgga cggactggaa agtttaacac
     2641 caaaagctat tgtagctgag cttgataaat atattattgg gcaaaacaag gccaagcgag
     2701 ctgttgccat tgctctgaga aaccgtaccc gaagactgaa attaccacag gaagttcggg
     2761 atgaggtagc tcctaaaaat atcttgatga ttggtcccac tggggtaggt aaaaccgaaa
     2821 tagcccgacg tcttgccaag ctttgtgggg ctccctttct gaaggttgaa gcgacaaaat
     2881 acacagaagt tggctatgta ggacgcgatg tagagtcaat gattcgtgat ttaatggcta
     2941 ccggcttttc tatggtaaag tctgagatgc aaagcacttt gcgaggagag gcagaaaagc
     3001 gggccgagga agttcttttg gatttactct tgccgggtat aaaaaagaaa acagaagcct
     3061 acaaaaccac acatgaacat ggtttgatta tcgaggatgg ttcagttgag aaatctcccg
     3121 tacctcctgt tgttcccgct gcttctaccg agtcagttga tacacgcgag aaatttcgtc
     3181 ttatgcttcg tgagggaaaa cttgatgagc gtgtggtcga gattactgtg caacgcggag
     3241 gaatgccgaa tcttgaggtg tttgcaggct ctgcaaatct tgaagatctg gagatgagtg
     3301 ttgccaatat taccaatatg attaccggta accgaaataa gcgcaaacag gtaacagtaa
     3361 aagatgcaag agaaataatc atcgcagaac agcttgataa gatagttgat caggataaaa
     3421 t
//
LOCUS       DAOQXQ010000105         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29139_length_3404_cov_2.450284, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000105 DAOQXQ010000000
VERSION     DAOQXQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29139_length_3404_cov_2.450284"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKH81_02520"
     CDS             complement(<1..520)
                     /locus_tag="PKH81_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNQ96942.1"
                     /translation="MKEIPVVYLDQDILIVNKPYGLAVQGGAGISICLIDVLESQMGE
                     KIYPVHRLDRDTSGLMILCRSSRSAAMYTNILGQGETSKRYQAVCFGKPPRPSDRIIM
                     PAGKAGKRKSADTLYTLLGSTSDISLLDLELGTGRMHQIRLHLASIGCPIIADDKYGD
                     FPKNRSVRSEYGI"
     gene            535..1236
                     /locus_tag="PKH81_02525"
     CDS             535..1236
                     /locus_tag="PKH81_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96943.1"
                     /translation="MNTEKNHRQLAVRLLIVAVFLHIGFLLNGEQLVSTEWGYALDIP
                     EAYSLVAKSGTTRYHFSNTVKPVDLHIAIYPTTQFDKPEMALSSVQQQFQAEGKSVEF
                     TWLHRPASIGKLESERYEGWIASVALADNKGWMVLVAVTEPKSYVACEPMIISTLESF
                     FPDGQERIDSGPMMAFAWANQKEIIAEIIFQGKKLLIPLNEVDAEANQAVVDREFSLL
                     TAYLGHERVIPAWQR"
     gene            1243..1716
                     /locus_tag="PKH81_02530"
     CDS             1243..1716
                     /locus_tag="PKH81_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ96944.1"
                     /translation="MIYRDAWKRLERAGCIITGNVVSGEQQAAADILSWLQGFSYERN
                     REGSDFVNLVSTLTDARGDCDSRALLMVILLHNMGIDAILLVSPDYSHAVAAVDCPGE
                     GARYELGGKRYLIADTTAKAGFGMIAADMADSSKWFPVRFATFPEGKAVDTTPWK"
     gene            complement(1700..2353)
                     /locus_tag="PKH81_02535"
     CDS             complement(1700..2353)
                     /locus_tag="PKH81_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNQ96945.1"
                     /translation="MADDILTIEEVAKYLRVSERTVYDWAQKGEIPAGKIGTVWRFKK
                     DEIEKWVNERLTSSVKNPQSSPLVQVQNILSPDRIVILNHSTRHDALVALSDVLGSAP
                     QIKNRQELTVEILKREELMSTAIGRGIAIPHVRLSSVTDLVMAVGLCKHDIIDFQTID
                     DIPVRLLFMIAAAYNQHSYYLQTLSFFSTKLKSTSLREGLLAAASPIDAYNLLISKE"
     gene            complement(2461..2874)
                     /locus_tag="PKH81_02540"
     CDS             complement(2461..2874)
                     /locus_tag="PKH81_02540"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNQ96946.1"
                     /translation="MENAIEDTKLYKAAQEAAERAYAPYSHFRVGAAILLEDGQIITG
                     VNVENRSFGLTNCAERSAIYTAVSAGKKQFLALAVSTPDSNYPVSPCGACRQVLSEFM
                     NAQTPVIFGPNEHSLVHTTVGELYPFDALHELAQA"
     gene            2899..>3404
                     /locus_tag="PKH81_02545"
     CDS             2899..>3404
                     /locus_tag="PKH81_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96947.1"
                     /translation="MNIKGNLKYIISGSLLFLLIYLFIAAIPIGPDLYFEPVWTRDLG
                     TSAHTEISELPTHALSDSEAFILGPRFGYFTPEGKILANVETAGRASASAHAWCAYTE
                     TPQSTTVYFTGEFPPITIQEEGFVYLNDQRLYLFSPGGSCVSQYDRNGQKRWRREHNA
                     PITAFNSSP"
BASE COUNT          905 a          784 c          824 g          891 t
ORIGIN      
        1 gtattccata ttcagagcgg acgcttcgat ttttgggaaa atcaccgtat ttatcgtcag
       61 caataatcgg acatccaatt gatgcaagat gaaggcgaat ctggtgcata cgcccggtac
      121 ccagctccaa atcaaggagt gatatatcag aggtgcttcc caacagggta tacaaggtat
      181 cggcgctttt tcttttgcca gccttgcctg cgggcatgat aatacgatct gacgggcggg
      241 ggggctttcc aaaacaaacc gcttgatagc gctttgatgt ttcaccttgc cccagtatgt
      301 tggtatacat cgcagcagat cgagaactgc ggcaaagaat cattaggcct gaggtatccc
      361 ggtccagacg atgcaccgga tatatttttt cgcccatctg gctttccaga acatcaataa
      421 ggcagatgct tattccggca ccgccttgta ctgcaagccc atatggttta ttaacaataa
      481 ggatatcctg atcaagataa acaacaggga tttctttcat ggagggattc tataatgaat
      541 acagaaaaaa atcatcgcca attggcggtg cgactgttga ttgtggcagt attcctgcat
      601 atcggcttct tgcttaacgg ggagcaactt gtttccaccg aatggggcta tgccctggat
      661 attccagaag cgtattcgct ggtcgctaaa agcggtacca cgcgatatca tttttccaat
      721 accgtaaagc cagttgacct gcatattgcg atatacccca ccactcaatt tgataaaccc
      781 gaaatggccc tctcctctgt gcagcaacag tttcaggcgg agggaaaaag cgttgagttc
      841 acctggttac accgtcctgc gtctatcggc aagttggaaa gcgagaggta cgagggatgg
      901 atcgcatctg tcgccctggc ggacaacaag ggttggatgg tattagtcgc tgtaaccgag
      961 ccaaagagct atgttgcatg tgaaccgatg attatttcga cgcttgaatc gttttttccc
     1021 gacggacaag aaagaatcga ttcagggcca atgatggctt ttgcctgggc aaatcaaaag
     1081 gaaataatcg cagaaattat attccagggg aaaaagctcc tgattccgct caatgaggta
     1141 gatgccgagg caaatcaagc ggttgtggat cgagagtttt ccctgttaac agcatatctt
     1201 ggccatgaac gggtaatccc ggcatggcag cgatagtacc gcatgattta ccgagatgcc
     1261 tggaagcggc tggaacgcgc cgggtgtatc atcacaggaa atgtagttag cggggaacag
     1321 caagccgctg ccgatatact ttcgtggctg caggggttca gctatgaacg gaacagggaa
     1381 ggctctgatt ttgtaaatct ggtaagtacc ctaaccgatg cacgagggga ttgcgacagc
     1441 cgcgctctgt taatggtgat actccttcat aacatgggta ttgatgccat cttgctggta
     1501 tctcctgact atagtcatgc agtagctgcg gtggattgcc cgggagaggg agctcgttat
     1561 gaattgggag ggaaacggta cctgattgca gacacaacag ccaaggcagg cttcggtatg
     1621 atcgctgccg acatggcgga tagctcaaag tggtttccag ttcgcttcgc aacctttccc
     1681 gaaggaaagg ctgtggacac tactccttgg aaataagcag gttataggca tcaattggtg
     1741 atgccgctgc aagcaagcct tcccggagag aggtactctt tagcttggta ctaaaaaatg
     1801 acagtgtttg caaataatat gaatgctggt tataagccgc ggcaatcatg aataacagtc
     1861 tgacaggaat atcgtcaatc gtttgaaagt caataatatc gtgcttgcat aagccaactg
     1921 ccataaccaa atctgttaca gaggaaagcc ggacatgcgg gatagcaatg cctcgcccga
     1981 tagcggttga catcagctct tcccgtttga gtatctcaac cgtcaattcc tggcggtttt
     2041 ttatttgcgg agcgcttcct agcacatcag aaagtgctac caatgcgtca tggcgagttg
     2101 aatgattaag tataacaata cgatcgggtg acaagatatt ttgaacttgt acaagagggc
     2161 ttgactgagg gttctttacc gagctggtaa gtcgctcatt aacccatttt tcaatctcat
     2221 ccttcttgaa tctccacaca gtaccgatct ttccggcagg gatttcacct ttttgtgccc
     2281 agtcatatac ggttcgctct gacactcgca ggtattttgc cacttcttct atagtaagga
     2341 tatcatctgc caagtttgta tctcccgata tctattttca aatattctgc atcataccgc
     2401 actatatgtc aagcattatc atcagggatg cttattcaga aagaaacgca aggattcttg
     2461 tcaggcttga gcaagctcat gaagggcatc aaaaggatag agctcaccga cggtggtgtg
     2521 aaccaaagag tgttcattgg gaccaaagat aacgggagtt tgtgcattca taaactcgga
     2581 tagaacttgc cgacaggctc cacaaggact taccggataa ttggaatcgg gagtcgaaac
     2641 cgccaaggca aggaattgct tctttccagc tgagacagct gtatatatag cgctccgttc
     2701 agcacaatta gtcaggccaa aagacctgtt ttccacatta acgcccgtaa tgatttgacc
     2761 atcctccaga aggatggccg cgccaactcg aaagtgtgaa tatggggcat acgcgcgttc
     2821 tgcagcctct tgtgcagcct tatagagctt ggtatcttct attgcatttt ccatagggct
     2881 agtataccat tgtacctcat gaacatcaaa gggaatttga agtacatcat ctctggatcc
     2941 ctcctttttt tactaatata tttgttcatc gctgccattc caataggacc tgatctgtat
     3001 tttgaaccag tgtggacacg cgatttagga acatcggcgc acacagagat tagcgagttg
     3061 ccaacacacg cactgagcga cagcgaggct tttatcctgg gccctcgctt tggttatttt
     3121 accccggaag gaaaaatcct tgcaaatgta gaaactgctg ggcgagcatc cgcttcggca
     3181 cacgcatggt gtgcgtatac tgaaacacct caatcgacaa cggtttactt taccggtgag
     3241 tttccgccca taaccatcca agaagaaggc tttgtctatc taaacgacca acgcttgtat
     3301 cttttttctc cgggcggtag ctgcgtaagt caatatgatc gcaatggtca aaaaagatgg
     3361 aggcgtgagc acaatgcgcc tattaccgcc tttaattctt ctcc
//
LOCUS       DAOQXQ010000106         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29226_length_3397_cov_3.458707, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000106 DAOQXQ010000000
VERSION     DAOQXQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29226_length_3397_cov_3.458707"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1133)
                     /gene="trxB"
                     /locus_tag="PKH81_02550"
     CDS             complement(171..1133)
                     /gene="trxB"
                     /locus_tag="PKH81_02550"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNQ96948.1"
                     /translation="MAQNKVYDMVIIGAGAAGLSAAQYAGRSNLKTLVIEEKSEGGQA
                     VLIDKLENYPGILEPVNGYDFASNMKEQAIRFGAQFTYGKVTGLGRKEDFFLVPLEGE
                     NSEGETIKARTVLLATGAEHRHLGVPGETEFYGKGVSYCATCDGPFFRDRHIVVVGGG
                     DAACDEAGFLAHITDRVTIIHRKSSFRAQKALAERTLKNPHIRVRFNTIVNEILGKDG
                     KVSAVRVQNSDSGETEEIACDAVFIFIGMIPRTELASLAKKDETGYLITDEHMQTSIR
                     GLFAAGDIRAKPFRQIITAASDGAIAAHSAASCIDEQMCGMGAQ"
     gene            complement(1152..1730)
                     /locus_tag="PKH81_02555"
     CDS             complement(1152..1730)
                     /locus_tag="PKH81_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96949.1"
                     /translation="MHSHRGKYSKSGLEWQGQSQLFFLFAKPAIASIIFEKPERIIMY
                     ELKPGIVAQKRGKNAFYILTWSALTKADKYEVTLKVPSVSGLYELYSMDKEKKLNLLS
                     VTHAWYGGLRSEIRAHIDPDATSDPVRKASLEDAELYYRYAPSDNFPDIQDVVWFLHS
                     TYFPHDIRVEHSGRYERIHLSEKAPDRVSWLE"
     gene            1738..1809
                     /locus_tag="PKH81_02560"
     tRNA            1738..1809
                     /locus_tag="PKH81_02560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1770..1772,aa:Cys,seq:gca)
     gene            2109..3251
                     /locus_tag="PKH81_02565"
     CDS             2109..3251
                     /locus_tag="PKH81_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ96950.1"
                     /translation="MSRRIASLPSRIRLFSREYGALPRTLLLTGSRGVGKTTFLLHHA
                     RESRMLYFSADNPMLAGELLYETVKSMFMVGYEGVIVDEVHYARDWSLHLKALYDDFP
                     AHRLWVSDSSALVLRAALGDTSRRYVPIRMPLLSFREFLYLETGVDYPVCDAFSGSVK
                     LPVEPTSAILDSFRKYRSFGTRPFYGEGNFEDRMLAVLDKTLYSDVPFFLPNITDGNL
                     RLMKAITGTLAAASIPRLQVRSLCADWGVGADKLYQILGVMESVGVLRIVRTENDTKA
                     KSAGDKLFFADPAFYSVLRGSLGTSREALVASLCADAGWTVETTKDDSTGDFVITRPS
                     VRGSEKLKIEVGGASKKIKSADFVIRDDIDYPAGNTVPLWLLGMGW"
BASE COUNT          793 a          866 c          885 g          853 t
ORIGIN      
        1 catttcttcg tccttcatcg agtgaacgat acgggtggca agaatcacat cttcctccgt
       61 atcccaaaaa ttgggtaaag agggatcgga gacagaagag gcagtaaaaa ggagaaaggc
      121 ggcaagcagg catacacaag aagcaataaa ccgcctaaag gacacagata tcattgagcg
      181 cccatcccgc acatctgctc gtctatgcac gaggccgcgc tatgtgcggc gattgcccca
      241 tccgaggcag ctgtaataat ttggcggaat ggtttagccc gaatgtcccc cgcggcaaac
      301 agaccgcgga tggaggtttg catatgctca tcggtaatca agtagcctgt ttcatctttt
      361 tttgccaggg acgcaagctc cgtacgggga atcataccga taaagataaa caccgcatcg
      421 caggcgattt cttcggtttc gccgctgtcg ctgttttgaa cgcgcacggc gcttacctta
      481 ccgtccttgc ccagaatctc gttaacaata gtgttaaaac gtacccgaat gtgcgggttt
      541 tttaaggttc gctctgcaag ggctttttgg gcacggaagg aagatttgcg atggataatg
      601 gtaacgcgat cggtaatatg tgctaaaaaa cccgcttcgt cgcaagccgc gtcgccgccg
      661 ccgacaacca caatatggcg atcccggaaa aagggcccgt cgcaggtggc gcagtacgaa
      721 actcccttgc catagaattc ggtttcgccg ggaaccccca ggtggcggtg ctcggcgcca
      781 gttgcaagaa gtactgtgcg agccttaatg gtctcgcctt cggagttctc tccttccaga
      841 ggaacaagaa aaaaatcttc tttacggcca agcccggtaa ccttgccata ggtgaactgt
      901 gcaccaaagc gaatggcttg ctccttcata ttcgacgcaa aatcgtagcc attaaccggc
      961 tctaaaatcc ctggatagtt ttcaagcttg tcgattaaca ccgcctggcc accctcgctt
     1021 ttttcttcga tgaccagggt ttttagatta gaccgtccgg cgtattgagc tgcggaaagg
     1081 cctgccgccc ccgcgccgat aataaccatg tcatacacct tgttttgagc catagagagc
     1141 ctccttggtt gttattcaag ccaggacacc cggtccgggg ccttttcgga aagatgaata
     1201 cgctcataac gacccgagtg ttctacccgt atgtcatgag gaaaataggt tgaatggaga
     1261 aaccacacca catcctggat gtcgggaaag ttgtcggacg gagcatagcg gtagtagagt
     1321 tccgcgtcct caaggctcgc cttgcgaacc gggtcactcg tagcatcagg atcgatatga
     1381 gcgcgaatct cgctcctgag tcccccgtac caggcatggg taaccgacaa gaggttcagt
     1441 tttttctctt tatccatgct atacagttca taaagaccgg aaacggaggg cactttaagg
     1501 gttacctcgt atttgtcggc tttagtcagg gcagaccagg tgagtatata aaaagcgttt
     1561 tttccgcgtt tttgcgccac tattccgggt tttagctcgt acatgatgat cctttcaggc
     1621 ttctcaaaga taatactcgc gattgccggc tttgcaaaga ggaaaaacaa ttgggactgc
     1681 ccttgccatt caaggcccga tttgctatat ttaccccgat gtgaatgcat tctacatggc
     1741 gccgtaccca agcggtaagg gagaggtctg caaaaccttt atgcatcagt tcgattctga
     1801 tcggcgcctc gtttaaagcc gttccggtaa ccccggagcg gctttttttt tgctgctatc
     1861 aaaattgttg aagactgcta ccaaaattgt tgaaaaaatc tccggttttt ttgtttgcga
     1921 catcctacgg gatcttgcgc tggaaaatcc ccatggctct gtttgtggtt tagtggaaga
     1981 aacgtccaaa acggtcatcc tgttcctgct gtaggaacaa aaaggcaaaa atgttcttga
     2041 cgcaggaacg acggggtgct accatagtag atatggatgc tcgcactata accgaactcg
     2101 agactgccat gagccggcgg atcgcttctc ttccttcacg aatcaggctg tttagccggg
     2161 aatacggggc acttccccgt accctccttt taaccggttc ccggggcgtc ggtaaaacga
     2221 cctttctcct gcaccatgcg cgggagagcc ggatgctcta tttttccgcg gacaatccga
     2281 tgctcgcagg agagctgctc tacgaaacgg taaagtccat gttcatggtc ggctacgagg
     2341 gcgtgatcgt ggacgaggtc cattatgcac gtgactggag ccttcacctg aaagcgttgt
     2401 atgatgattt tcctgcacat cgcctctggg taagcgattc gtccgccctt gtcctacgcg
     2461 ccgctcttgg agacacctcc cgccgctatg taccgatccg catgccgctt ctttcattcc
     2521 gcgagtttct ttatctcgag acaggtgtcg actatcctgt gtgcgatgca ttcagcggtt
     2581 cggtgaagtt gcctgtagag ccaacgtctg caattcttga ttccttcagg aagtaccgtt
     2641 ccttcggaac gcgtccgttt tatggcgaag gtaattttga ggatcgcatg ctcgccgttc
     2701 tcgataaaac cctttactcc gacgttcctt tttttctacc gaatattacg gacggaaatc
     2761 tcaggcttat gaaggcgata accggcacgc ttgccgccgc aagcattccc cggctccagg
     2821 tgcgctctct ctgcgcggac tggggcgtcg gggccgataa gctttatcaa attctgggcg
     2881 taatggaatc ggtcggcgtt ttgcggatcg tgcggaccga gaacgatacc aaggctaaat
     2941 ctgccggcga caaacttttt ttcgccgacc ctgcttttta ttccgtgctt cgcggaagtc
     3001 ttggaacctc gcgcgaggcg ctcgtggctt ctctctgcgc cgacgctgga tggacggtgg
     3061 agacaaccaa ggatgactcg acgggtgatt tcgtaattac gagaccgtct gtgcggggca
     3121 gtgagaagct gaagatcgaa gtgggcggcg cctcgaagaa gatcaagagc gccgacttcg
     3181 tcatccgcga tgacatcgat tacccggctg gcaacacagt accgctgtgg ctattgggga
     3241 tggggtggta atgaatgtag ctaactttaa tgaaaaaatt agatttatcg ttgaaaacgg
     3301 agatctgtca ataaacggag tgctttatcg cgagtatagt gatgcctttg atgaatggta
     3361 tggagatcca atatggtacg gcgatccaaa atcgtat
//
LOCUS       DAOQXQ010000107         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29339_length_3390_cov_2.851574, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000107 DAOQXQ010000000
VERSION     DAOQXQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29339_length_3390_cov_2.851574"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..837
                     /gene="lptB"
                     /locus_tag="PKH81_02570"
     CDS             109..837
                     /gene="lptB"
                     /locus_tag="PKH81_02570"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ96951.1"
                     /translation="MSDTLRVEGLNKYFGKKHAVRDVSFSMKAGEILGLLGPNGAGKT
                     TSFYMVVGFYKPHSGSILLNDVSIASLPMYKRALLGLSYLPQEASVFRKLTVEQNIWA
                     ILETRKDLTEAEKRNKLDFLIDEFGINANRKQPAYTLSGGERRRTEIARSLAIEPKFL
                     LLDEPFAGIDPIAVHDIKGIIRLLSNQGIGILITDHNVRDTLEITVRACIINKGQIVE
                     EGPRDYILSSEIARQVYLGEEFRM"
     gene            complement(810..2258)
                     /locus_tag="PKH81_02575"
     CDS             complement(810..2258)
                     /locus_tag="PKH81_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96952.1"
                     /translation="MTRSRLGQAFLTALVLFLTACSTGIGSSDEDSSRKIEVYGNLYI
                     PIPSDFLFVSELTDSEQRIKGLTGDILITVGSQELIIPAGKTYLKDSQTLAAPLPANL
                     TIDFYGVAVITCMSRLSTDNTTLSFAGYLLTSIDKNAKTLDLNNIDLDGDKIISSQEK
                     SSPQFIPAVKSNGYTAVALASISRFQAKTLNPSTPTAELFLTAYGLDWNLAFIGVSDG
                     KGHQEFRFIDALTLLDSGLLSIDSTDVDPFKIPLVRDSSATSGTNTLSLYIVNSKGLS
                     TSSPLTLAWSSDSIKGKENFLVTLTWDNMGDLDLHVWFKHNTDDTQNWHACWGLYSDI
                     PFYDGAGVINLDIDNENGFGPEHFSLFGARNGRYAIAVNANKASKTDVVTSLQPLSQP
                     VNAFVTIETNHALKSYGPIRFSHVNGETYPVEGSPAWKRIIDIEVKDGRASFLEPNLA
                     WALPQEGGHVPGYINVYSKLAKKPYILNSSPR"
     gene            2374..>3390
                     /locus_tag="PKH81_02580"
     CDS             2374..>3390
                     /locus_tag="PKH81_02580"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNQ96953.1"
                     /translation="MKITVLAGGLSPERDVSLSSGSLIANALIDKGHQVALVDVYEGL
                     SVPFDGVSSLFYAAADGHQFSYKVPEKEPDLEDIRRKNGNRRELVGPGVLDLCRASDV
                     VFLALHGAMGENGQIQAFFDVLGIPYTGSGYAGCLLAMDKDVTKRLLVRDGIPTADWV
                     LVQPDREDRHTMLERIRGEIGFPCVVKPLSCGSSVGVSMVTAYEELEKALDEASLWES
                     RILVEKKITGREFSIGIFLDRALPSIEIIPVQGFYDYANKYQGGRSREICPADLPPQE
                     ESTLATLALSVHRCLQLGSYSRIDFIRDEKGVFWCLEANTLPGMTPTSLLPQEARATG
                     ISYGD"
BASE COUNT          845 a          693 c          933 g          919 t
ORIGIN      
        1 tctcttgacg gctcggtaac aggaaaactg gaagacacca aggaagagac caaggatgag
       61 accaaggaaa acgatgagga acagtgagtg gacggccagg gagggcagat gagcgacacc
      121 ttgcgggttg aaggattaaa taaatacttt ggaaaaaaac acgcggttcg cgatgtgtca
      181 ttttccatga aggctggcga aatcctaggg ctgctcggtc ccaatggtgc agggaagaca
      241 acctcgtttt atatggttgt aggtttttat aagccccatt cgggttccat tttgcttaat
      301 gacgtaagta ttgcttcttt gcccatgtat aagcgtgccc ttcttgggct ttcgtatctt
      361 ccgcaggaag cctccgtgtt tcgtaaactc actgttgaac aaaacatatg ggcgattctt
      421 gaaaccagaa aggatttgac tgaggcagaa aagcgaaaca agcttgattt tttgattgat
      481 gaatttggta ttaatgcgaa cagaaagcaa cctgcgtaca ctctttccgg gggtgagcgc
      541 cggcgaacag aaatagctcg gtccttggca atagagccca agtttcttct tttggatgaa
      601 ccttttgcgg gtattgatcc gattgccgtg catgatatta aagggattat caggctgtta
      661 tccaatcagg gtattggaat tttgataacc gatcataacg tgcgggatac cctggagata
      721 accgttaggg cgtgcattat caacaaggga caaattgtgg aagaaggacc gagagactat
      781 attctttctt ccgagatagc acgacaggtt tatctggggg aagaattcag gatgtagggc
      841 ttttttgcca gtttagaata cacattgata tatccgggta cgtgtccgcc ttcctggggc
      901 aaggcccagg caaggttcgg ttccaggaat gatgcccgcc catctttaac ttcaatgtcg
      961 atgattcgct tccatgcagg agagccttct accgggtagg tttccccgtt aacatggcta
     1021 aatctgatag gtccgtagga tttgagagca tggttcgttt cgatggtaac aaaggcgtta
     1081 accggttggc tcaagggttg caggctggtt acgacgtctg ttttggaggc cttgttcgcg
     1141 tttaccgcga tggcatagcg accatttcgg gcgccaaata gagagaaatg ttcgggccca
     1201 aagccatttt cgttatcaat atccagattt ataacgcccg ctccatcgta gaagggtatg
     1261 tctgaatata aaccccaaca ggcatgccag ttctgcgtgt cgtcggtatt atgtttaaac
     1321 catacatgaa ggtccaggtc tcccatgttg tcccaggtca gggtaacaag gaaattttct
     1381 ttgcccttga tcgagtccga gctccaggcg agggtaagcg ggcttgaggt tgaaagaccc
     1441 tttgaattaa caatgtacag agaaagggta tttgttcctg atgtagccga ggagtcccgg
     1501 accaggggaa ttttaaaagg atccacatcg gtggaatcga ttgagaggag ccctgaatca
     1561 agaagggtaa gggcatcgat aaaacgaaat tcttgatgtc ctttcccgtc gcttacaccg
     1621 ataaaggcaa ggttccagtc gagtccatag gcggtaagaa agagttccgc ggtgggagtg
     1681 gatggattga gggtcttggc ctgaaagcgt gaaatagagg caagggcgac tgcggtatat
     1741 ccgttgcttt ttactgcagg aataaattgg ggactcgatt tctcctggga gctgattatc
     1801 ttgtcgccat ccaggtcaat attgttaaga tccagcgttt tagcgttttt gtcgatggag
     1861 gtaagcaggt atcctgcaaa ggagagggtt gtattgtctg tagacaagcg ggacatacag
     1921 gtaattaccg ctaccccgta aaagtcaatg gtcaggtttg caggaagagg ggctgcaagc
     1981 gtttgggaat ctttgaggta ggttttaccg gctggaatga taagctcctg agatccaacc
     2041 gtaataagaa tgtctccagt aagtcccttg attcgctgtt ctgaatcggt gagctcggat
     2101 acaaacaaaa agtccgaagg aatagggata tacaggtttc cgtagacttc gatttttcgg
     2161 gagctgtctt cgtcggagga gccaatgccg gtagaacagg ccgtgaggaa aagcactaag
     2221 gctgtcagaa atgcctgacc aaggcgcgat cgtgtcattg tctgtttcct ccttaatacg
     2281 gcgttatgaa tcaagtaaat tcaagtaatt gtaaagcatt taccgccaac tatcaattgt
     2341 ttttcccttc tctttatgat ttactgatac atcatgaaaa taacggtact tgcgggcggt
     2401 ttgagcccgg agcgagacgt ctcgctgtct tctggaagtc ttatcgcaaa cgcgcttatt
     2461 gataaaggtc atcaggttgc cctggttgat gtgtatgaag ggctttccgt tccgttcgat
     2521 ggagtgtcct ccctgtttta tgctgcggct gatgggcatc aattttccta caaagtaccc
     2581 gaaaaagaac ctgatttaga ggatatccgc cggaaaaatg gtaatcgccg ggaattggtt
     2641 ggcccgggtg tcctggactt gtgtcgggcc agtgatgtgg tttttctggc cctgcatggc
     2701 gcgatgggag aaaatgggca gatacaggcc ttttttgatg tgttgggcat tccgtacact
     2761 ggctccggct atgctggttg ccttttggca atggataagg atgtaacgaa gcgattactt
     2821 gtccgggacg gaattccgac ggctgattgg gttctggttc agccggatcg ggaagatcgc
     2881 catactatgc ttgagcgtat tcgcggggaa attggctttc cctgcgtagt taagcccctt
     2941 agttgtgggt caagtgtggg tgtttccatg gttactgcct atgaagagct tgaaaaagcc
     3001 cttgacgagg caagtctgtg ggagtccagg atcctggttg agaaaaaaat cacggggaga
     3061 gagttttcga ttggtatctt tcttgatcgg gcactaccgt caatcgagat tatccccgtt
     3121 cagggctttt atgactatgc aaacaagtat caaggtggtc gttccaggga aatttgcccg
     3181 gctgatctgc ctcctcaaga agaatcgacg cttgccaccc tcgctctttc tgtgcatcgc
     3241 tgtttacagc ttggaagtta ttcaagaatt gactttattc gagatgaaaa gggtgttttt
     3301 tggtgtttgg aagcgaatac ccttcccgga atgaccccga caagcttgtt gccccaggaa
     3361 gcacgggcaa ccggcattag ttatggggat
//
LOCUS       DAOQXQ010000108         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29554_length_3372_cov_2.826952, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000108 DAOQXQ010000000
VERSION     DAOQXQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29554_length_3372_cov_2.826952"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1734
                     /gene="xylB"
                     /locus_tag="PKH81_02585"
     CDS             232..1734
                     /gene="xylB"
                     /locus_tag="PKH81_02585"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNQ96954.1"
                     /translation="MNTIAGIDMGTQSIKVVLYDWENRVTIAKSQVPVDIIAENDGTR
                     EQKTEWYDAALEACFASFTADQRASIKAIGISGHQHGFVPLNAEGKPLYNVKLWNDTS
                     TVEECKIITDAAGGNDAVIAEVCNLMLPGFTAPKILWLKRHKPEAFAKLAYIMLPHDY
                     LNWLFTEKYIMEAGDASGMALFNGITKTWSEKICSIIDPSLTKLLPSIIASNAPAGFV
                     TPKAAKRFSIPEGIPVSSGGGDNMMGAIGTGTVRDGFLTMSLGTSGTLYGYSENPVSD
                     PVKGLSGFNSSTGGYLPLLCTMNCTVATEETRALFGMDVKEFDSCASKAPVGAEGVVF
                     LPFFNGERTPNLPNGRASIMGLSAANASRENIARAAMESAIFGMRIGLEAFQALGFRA
                     KEIRLIGGGSKSALWRNIAANVMNLPVKRPENDEAAAMGGAIQALWCLKTIEAAKNGT
                     TAPSIASLTDAHVTINEGDQVMPDPTAVAAYDKAYAQYNRYLTTLAPLYQ"
     gene            1833..3035
                     /locus_tag="PKH81_02590"
     CDS             1833..3035
                     /locus_tag="PKH81_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNQ96955.1"
                     /translation="MKRQLSMPFWCVRSTCGDPFGGSVMDSITPLEACDILCEAKKQG
                     LIDFTSTHDDDIVAWDPMNPMDDQDPSSECYKTIHALKEKMDKVGLKMIMVTCSLHGD
                     KLFRNGGLTNPDPEIRAMAAQKVMRTLRIGNFFGAEYFTYWVARDGFECQFAVDWKTP
                     YKYIEQGLNLATRYIKENKLSIKYCTIEPKPNEPRGEMYLATTGHALGLISRLEDPSM
                     WGVNPELLQHEGMTNLSASNAAGMAIHAGKLPFLHIGNQKAGQFDNDSPTLTGMDGLK
                     EMIGLGWILEKLEWKGHIEFDNHILRTDAAPGKDNAIQLRKDFVKFNVDAWRLVEKKA
                     EQLAKDKSLDKIRATVLGKDAAVTKALDSYDLKAIQSAKFDYTKLVETPVSNMNIDMA
                     FNKAIMGM"
     gene            complement(3100..>3372)
                     /locus_tag="PKH81_02595"
     CDS             complement(3100..>3372)
                     /locus_tag="PKH81_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96956.1"
                     /translation="EGYEVKSYRTELKEKNVTIDLNFNETTKDMVVIYTIKLRTFDEG
                     DAIAQIRDAVKLFIKENGFTNYKVLSPDVVTYSSTETVMKRFLHIN"
BASE COUNT          958 a          814 c          784 g          816 t
ORIGIN      
        1 actcatccga gccatgtgca tgataccttc catgccaatt aagacggtct ggagaatgtg
       61 agcgctcccc ggcatgaagg cggtacacaa aaacagtatc aagcagtttc atgcaataat
      121 catacaaaaa agtgataaaa aaatcaattt tagatataaa aaatcagctt cgcccatact
      181 cttgccacat atccacccct atactcaaga tacaggaaag gaggttgtta catgaatact
      241 attgccggca ttgatatggg cacacaaagc atcaaggtgg tgctgtatga ctgggaaaac
      301 cgcgttacta tagcaaaatc acaagtcccc gtcgatatca tcgcggaaaa cgatggaacg
      361 cgtgaacaaa aaaccgaatg gtatgatgca gcgctcgaag cgtgttttgc ttcttttaca
      421 gccgaccaac gcgcaagcat taaggctatt ggaatctctg gccatcaaca cggctttgtc
      481 cccctgaatg cagagggaaa gccactctat aacgttaagc tctggaacga tacctcaaca
      541 gttgaagaat gcaagatcat taccgatgcc gccggaggta acgatgcagt aattgccgag
      601 gtgtgtaatc ttatgcttcc cggctttacc gctccaaaaa tactctggct taaacgacac
      661 aaaccagaag cttttgcaaa gctcgcctac attatgcttc cacacgatta ccttaactgg
      721 ctctttacag aaaaatacat catggaagct ggggacgcct cgggaatggc cctgtttaat
      781 ggaataacca aaacctggtc agaaaagatt tgttcgataa tagatccttc ccttaccaag
      841 cttcttccgt caatcattgc ttccaatgca cccgctggat tcgtcacacc gaaggcagca
      901 aaacggttta gtattccaga gggtatccct gtctcttccg ggggcggaga taacatgatg
      961 ggtgccattg gtaccggcac cgttcgcgat ggttttttga cgatgagctt gggaacctcg
     1021 ggaaccctgt acggttattc agaaaatccg gtatcagacc cggtaaaggg tttatcgggc
     1081 tttaactcat ctaccggcgg atatctacct ctgttatgca ccatgaactg caccgtggcc
     1141 acagaggaaa ctcgtgctct ttttggcatg gatgtaaaag aattcgactc ctgcgcatca
     1201 aaagcccctg ttggcgccga aggagtcgtt tttctcccct tctttaacgg ggagcgtacc
     1261 cctaatcttc ccaatggacg ggcaagcatc atgggtttat ccgcggcaaa cgcctctcgc
     1321 gagaatatag cccgtgcggc gatggaatcc gccatttttg gcatgcgaat tggtcttgaa
     1381 gcctttcagg ctcttggctt ccgcgccaaa gaaatcagat tgattggtgg aggctccaaa
     1441 agcgcattat ggagaaatat cgcggctaac gtgatgaatc ttcctgtaaa aagaccggaa
     1501 aatgatgaag cggccgccat gggaggagcg attcaagctc tttggtgcct taaaaccatt
     1561 gaagctgcca aaaacggaac aacagctccc tcgattgcat cgctgaccga tgcgcatgtg
     1621 accataaatg aaggagatca ggtaatgccc gatcccaccg ctgttgcagc ctatgacaag
     1681 gcttatgctc agtataatcg ctatctaacg acacttgcgc cgctatacca gtgattcaaa
     1741 ccttgcttga agctgattta cgctttattg ttaataaagc atgcgataac gcgtgaaacc
     1801 atcacgcaat actttttgaa ggaaggaaag ctatgaaaag acaactgtct atgccgttct
     1861 ggtgcgtaag gagcacctgt ggagatccct tcggtggatc agtaatggat tcaatcaccc
     1921 cccttgaggc atgcgatatt ctgtgcgaag ccaagaagca aggacttatt gattttacct
     1981 caacccatga cgatgacatc gttgcttggg accccatgaa tccgatggac gatcaggatc
     2041 cttcaagtga atgctacaag accatccatg ccttaaaaga aaagatggac aaggttggac
     2101 ttaagatgat catggtaact tgctcacttc atggagacaa gctcttccgt aacggtggac
     2161 tcaccaatcc cgatccggag attcgtgcta tggccgccca aaaggttatg aggacgctcc
     2221 gcatcggaaa cttctttgga gccgaatact ttacctactg ggttgcccgt gatggattcg
     2281 agtgccagtt cgcagtagac tggaaaaccc cctacaagta catcgagcag ggactcaatc
     2341 tggctacccg ctacatcaaa gaaaacaagc tgagcatcaa gtattgcacc atcgaaccca
     2401 agcccaatga gccccgcgga gagatgtatc ttgcaaccac cggtcacgca cttggtctga
     2461 tcagccgcct tgaggacccc tcaatgtggg gagtgaaccc cgagcttctc cagcacgaag
     2521 ggatgaccaa cctctctgcg tccaacgctg cgggaatggc tattcatgca ggtaagcttc
     2581 ccttccttca cattggaaac cagaaggccg gacagttcga taacgactcc cctaccctta
     2641 ccggtatgga cggacttaaa gaaatgatcg gtttgggctg gattctggaa aagcttgagt
     2701 ggaaaggcca cattgagttt gacaatcaca ttctgcgcac cgatgctgcc cctggaaagg
     2761 acaatgcgat tcagctgcgt aaagattttg tgaagttcaa cgttgatgcc tggcgcctgg
     2821 tagaaaagaa ggctgagcaa cttgccaaag acaagagcct ggacaagatc cgcgcgactg
     2881 tattgggtaa ggatgcagct gtgactaaag ccctggatag ctacgatctt aaggctattc
     2941 agtctgcaaa gtttgattac acaaaattgg tagaaacacc agtttccaac atgaacatcg
     3001 atatggcctt taacaaggct atcatgggta tgtaagtcca ctggacaaga aaaaagccgc
     3061 cggaaacggc ggcttttttt ttaaggagaa aacaataaat caattgatgt gtaaaaaacg
     3121 cttcattact gtttcggtag aagaatacgt aacaacgtcg ggtgacagca ccttatagtt
     3181 agtaaagcca ttctccttga taaaaagctt aaccgcgtcc cgaatctggg caatagcatc
     3241 gccttcatca aaagtacgga gcttaatggt ataaatgacg accatgtctt tggttgtctc
     3301 attaaaattg agatcaatgg tgacattttt ttctttgagt tcagtacgat agctttttac
     3361 ctcgtaccct tc
//
LOCUS       DAOQXQ010000109         3369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29602_length_3369_cov_2.613760, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000109 DAOQXQ010000000
VERSION     DAOQXQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29602_length_3369_cov_2.613760"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="PKH81_02600"
     CDS             <1..2107
                     /locus_tag="PKH81_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ96957.1"
                     /translation="TTTQSKIREDATGFFTEYTHSLNAEIANYFTEIRGQLSIFTENP
                     DTARYNWGAIKEPMNRIAREHPEIDSFILVKKDGSYFRSDNAGNPALGGVVTADNKDP
                     AAKPTLLVSRDYFKRLVSESSSNQMESVISNPNLSRSTGKKQVLVASTLLDSQNNKAG
                     LLAASIPVDSLTAMLDTFSDNLIKYFSTEAQVVVLSDTGALVAYRKYDKASKTYAEQI
                     LTDPREALIDELPPPIHTSITSMMQSGERFSSFSMGGKRKIWHSTGSVIAGTNYYLYT
                     FLPDHVLFSTIYFFRQLALSIIAITAIVIFCIAIVFGRRVTKPLIKTADTLRDISEGS
                     GDLTFRIQTSGNDEIAAVGNFFNRFIETLHGMITLIREQSLSMDRVSGELVNRAEGIN
                     KGIQQIGKNVTDLNFQAEEQGASVTETSSTIHQIAKNIESLNTQIEGQSASVTESSAA
                     IQQMVSNINSISNNLERAGSGFENLLSASNNGRDSMQNVIELVKDVSIQSERLLETNE
                     IIDSIASQTNLLAMNAAIEAAHAGDAGKGFSVVSDEIRKLAESSSEQSKVIEAELKKA
                     VATIGTIVGASADADKAFGEVAGQIQEANGLIQEIRMAMKEQNEGSRQVLEALDEIQN
                     ITIQIRDGSLEMTQGAQMILKEMTRLEDISLKVQKSVQDIARSSDEIERAVAVVGESA
                     SKNHTIVAALSEVTDRFKL"
     gene            complement(2128..3282)
                     /locus_tag="PKH81_02605"
     CDS             complement(2128..3282)
                     /locus_tag="PKH81_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ96958.1"
                     /translation="MMQRNQIQSLAFIALLAVMLILMSSIFLPFAGVILWSAFLYILV
                     SPLYTRLIRLLTPGTRFYDVRRQIIAAVFALGTVTLVAGILVFLGFKLIGQVTGFVNQ
                     IKQFVQANPTFFQSSETGVYISDTVRRLSLGAVDISRINLKEEALSLLVTYSNTLVGL
                     TRSLAKNVSGFVLSLAFLCFSLYFFYADGAYLARVFIRAIPIDSKDTKRLLRKFREVT
                     RNLFMGFFLVAFYQSIAGFIIFSIFGINGSLLFAVLILFSSFIPIFGCAIIWIPLGLS
                     VFALQGVVPGVLFMLLCAFFISFLDNFLRPFFLQERVKIHPLLIFFSILGGLQVFGFN
                     GILLGPIVVILFFTTLDILLGEETEVSVKPGELPDDLLDGSPHGADPHKE"
BASE COUNT         1057 a          857 c          765 g          690 t
ORIGIN      
        1 aaccacaacc cagagcaaga ttagggaaga cgcgactgga ttctttactg aatacaccca
       61 ctcgctgaac gcagagatcg cgaactactt taccgaaatt cgcggccaac tttccatttt
      121 tacggaaaac ccggacaccg cccgctataa ctggggtgcg attaaagagc cgatgaatcg
      181 aatcgcccgg gaacatcccg aaattgactc gtttatcctg gtaaagaaag acgggtccta
      241 tttccgctcg gacaatgccg ggaaccccgc ccttggagga gtggtaaccg cagacaacaa
      301 ggaccctgcc gcaaaaccaa ccttattggt atcgcgagat tactttaagc gcttagtaag
      361 cgaaagctct tccaaccaaa tggagagtgt tatatccaac cctaaccttt cccgttctac
      421 cggaaagaag caagtactgg tggcctctac cctgctcgat agccaaaaca ataaagccgg
      481 gcttcttgcc gcctcaatcc cggtggacag ccttaccgcc atgctggaca ccttctcgga
      541 taatcttatt aagtactttt ccaccgaggc ccaagtggta gtccttagcg ataccggagc
      601 cttggtcgcc taccgaaaat acgacaaggc aagcaagacc tatgccgaac aaatccttac
      661 cgacccccgc gaagcgctta tcgacgaact ccccccgccc atacacacgt ccattacatc
      721 catgatgcaa agcggggagc ggttctcttc ttttagcatg ggcggtaaaa gaaagatatg
      781 gcactcaacc ggatcggtaa tcgcgggaac aaactattat ctatatacat tccttcccga
      841 tcacgtgctc ttttccacta tttacttctt tagacaactg gccctaagca ttatcgctat
      901 tacggctata gttatctttt gtatcgccat agtgtttggt cgcagggtaa ccaaaccgct
      961 tatcaaaact gcggacacac tcagggatat ctccgaaggc tcaggcgact tgaccttccg
     1021 catacaaacc tccggcaacg acgaaatcgc tgccgtcggg aattttttca accgctttat
     1081 cgaaaccctt cacggcatga ttaccctgat ccgggagcaa agtctttcca tggatcgtgt
     1141 ctccggagaa ttggtcaaca gagccgaggg aataaacaag ggaattcagc aaatcggcaa
     1201 aaacgttacc gatcttaact tccaggcaga ggaacagggc gcaagcgtta ccgaaacatc
     1261 atctaccatt catcaaattg ccaagaacat cgaaagtctg aacacccaaa tagaaggcca
     1321 gtcagcatcc gtaaccgagt catcggccgc gatacagcaa atggtgtcca atataaactc
     1381 tatttccaat aatcttgaac gggccggtag cggttttgaa aacctccttt ccgcgtcgaa
     1441 caatggccgg gacagcatgc aaaacgttat tgagctggtt aaggatgtgt ctattcaatc
     1501 cgagcgcctt cttgaaacca acgagattat cgattcaatc gccagtcaaa caaacctgct
     1561 tgccatgaac gcggcaatcg aggccgctca tgccggagac gcaggaaagg gcttctcggt
     1621 cgtttccgac gagattcgta aactggcaga aagttcatca gagcaatcca aggtcatcga
     1681 agcagagctt aaaaaagcgg tagcgactat cggaaccatt gttggcgctt ctgccgacgc
     1741 agacaaggcc tttggcgagg ttgcagggca gatacaggaa gcaaacggcc ttattcaaga
     1801 aattcgcatg gcgatgaagg aacaaaacga gggcagccgt caggtgcttg aagccctgga
     1861 cgaaatccag aacatcacca tccagattcg cgatggctct ctggaaatga cacagggagc
     1921 gcaaatgatc ctgaaagaga tgacgcgact ggaagacatc tcccttaagg ttcaaaagag
     1981 cgtgcaagac atagcccgct cgagcgacga gattgaaagg gccgtagccg ttgttggaga
     2041 gtccgcgtcc aagaatcaca caatcgtcgc cgccttaagc gaggttaccg accggttcaa
     2101 actctaaaac acgcagagtg ttgatcccta ctctttgtgg ggatcagcgc catgcggcga
     2161 accatcaagc aaatcatccg gaagctcccc tggctttacc gacacttccg tctcttctcc
     2221 aagcagtata tcaagggttg taaagaacag aataacaaca atcggcccta acaaaattcc
     2281 attaaaccca aacacctgaa gtcctcccaa gatcgaaaag aaaatgagca gggggtgaat
     2341 ctttacgcgc tcttgtaaaa agaaggggcg aagaaagtta tccaaaaagc taataaaaaa
     2401 ggcgcacaaa agcataaaaa gaacaccggg caccacgcct tgcaaggcga atacgctcaa
     2461 gccaagcggt atccaaataa tagcgcatcc aaatattgga ataaacgaac taaaaagaat
     2521 caggacggca aaaagtaaag atccattgat gccaaaaatc gaaaagataa taaaccctgc
     2581 aattgactga taaaaggcaa ccagaaaaaa acccataaac agattccgag tcacttcccg
     2641 aaacttgcgc aacaaacgtt tagtgtcctt tgagtcaatg ggaatcgcgc ggataaacac
     2701 tcgggccaga tacgccccgt ccgcgtaaaa gaaatacagg gagaaacaga gaaaggcaag
     2761 agacaaaaca aaaccggata cattttttgc cagactgcgc gttaggccaa cgagcgtatt
     2821 tgaataggtt accaacagcg aaagggcctc ttcctttaaa ttaatgcgag agatatcaac
     2881 agcgccaagg gagagacgcc tgacagtgtc ggagatatat actccggttt cggaagactg
     2941 aaaaaaagtg ggattcgcct gaacaaactg ctttatctga tttacaaaac ccgtaacctg
     3001 accaatcaat ttgaatccaa gaaaaacaag gatgccagca acaagggtta ctgttcccaa
     3061 tgcaaaaaca gcagcgatta tttgccgccg tacatcataa aaccgcgttc ccggagtaag
     3121 gagtcgaatg agtcgggtgt acaggggact taccaggata tacaaaaatg ccgaccacag
     3181 gattaccccg gcaaagggca aaaagatact gctcatcagg ataagcataa cggcaagaag
     3241 ggcgataaac gcgagagatt gaatctggtt gcgctgcatc atgccttgta ctatatatcc
     3301 ttacgagtaa cttgcaaacc ctggccaatc gtagtagtat tagttgcatt tagaaactaa
     3361 ccggaggca
//
LOCUS       DAOQXQ010000110         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29747_length_3357_cov_3.847971, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000110 DAOQXQ010000000
VERSION     DAOQXQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29747_length_3357_cov_3.847971"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PKH81_02610"
     CDS             complement(<1..1548)
                     /locus_tag="PKH81_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNQ96959.1"
                     /translation="MILYIATFVVSCSPSGVQAQKWPVTNACASLRGYTTPVPLEGDW
                     AFWFGTFIPPELSIPESASFARFPSSWSEYSQSFPRKGHASYAVRIEGLDPSVSYALR
                     LPGYSSACRYFINGKQIYSCGLPSENPVEEQWGWDSQVVMLPLGLSSCVLTLHISNYS
                     DVFPASQTAILIGPSRAIRHAYITKRISIIIPFGAILAMGAYFLALFAVHRQERSCLW
                     LGLLSCIFALRSLCYDEFLLQDIFPGVSTSLMFRLGYLTYSLAVGGFSGFISSNFPGL
                     ANRKIVAIIVSVSLLYALMNLVTPPSVFTAALLPFQVFSLLSAVYALALVIRAARRGR
                     EGALLFLLSFAVFFLVVIRDIAIANRIIRGVFLSHYGILGIIFVMALIIVRRFSGAFT
                     RLESASAELARVNESLERFVPNQFLQYLGKSSITDISLGDSVRRDMCVMFLHLGVDAP
                     LSGTLDRLNMLEFFNETLQRINPVISRHGGFVDKYLAEGCMVLFPDDSNAALGCAQEL
                     ARLMRQYN"
     gene            1641..2447
                     /locus_tag="PKH81_02615"
     CDS             1641..2447
                     /locus_tag="PKH81_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ96960.1"
                     /translation="MKRIQIITGASSGIGREFALQMARNRKADEIWLVARRRDRLESL
                     AAEIALIDGAAKPLVVEIDISGKAGATKLAKTLEREGQSGDLTIDALINNAGFGTYGP
                     FADTPLEKELEMIDLNITSLTALCHHALPYLAGGSLVVNVASLAGFIPLGNFAVYAAS
                     KAYVLSFSVALAAELADRKIFVSTVCPGPVDTEFALVASSGAREKVVDGKSAEAVVRA
                     CLTDAQRGKRISVMAWKWKFKAFMSRFVGRYWFARHTFVHEKRPSRSDGV"
     gene            complement(2448..>3357)
                     /locus_tag="PKH81_02620"
     CDS             complement(2448..>3357)
                     /locus_tag="PKH81_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96961.1"
                     /translation="AELFFVFQHGAADGIAATWFIHDFLTVLGGGQVDAPASPNMPTL
                     YEALKEEVTAELLTRPEPDWKKEDPPPPEPFDMPPYTVPDFFLRFFTLSPAGTARLAS
                     EAKAAGQTVNSFLGARVLKASADIFASEGNTTRTFQCPVDFRQYLKEKYRSVNGVYNG
                     IVKVPVDCSQDLAVIAETIRAGVLAQREGMKDVEEYFHFRDSFDNVADPESLMMGFPP
                     DPMDYDFSFSNLGRTVLAPSYGPFVLQDFYGPVFTAVNGETVIGLNTTNGSLRMSLIF
                     DKKIDKAAGYLQLGNRIQEIFTSFED"
BASE COUNT          959 a          841 c          874 g          683 t
ORIGIN      
        1 gttgtactgt cgcataaggc gggcaagttc ttgtgcacat cccaaagccg cgttactgtc
       61 gtccgggaaa agaaccatac agccctcggc caggtactta tccacaaagc ccccatgccg
      121 actaattacc ggatttatac gctgcagggt ctcgttaaaa aactccaaca tattgagtcg
      181 atccaaggta ccgcttaaag gtgcgtctac accaagatga agaaacataa cacacatatc
      241 acggcgaacg ctgtctccca gcgatatgtc cgtaatagag gactttccta agtactgtaa
      301 aaattgatta ggcacaaatc gttctagaga ttcgttaacc cgcgcaagtt ctgccgaagc
      361 actttccaaa cgggtaaacg ccccggaaaa tcgcctgacg attataaggg ccataacaaa
      421 aataatgcca agaattccgt aatgagacag aaaaacccca cggataatcc ggttagctat
      481 cgcgatatcg cgaataacca caagaaaaaa aaccgcgaag gataacaaaa acagcagtgc
      541 gccctcccgg ccacggcgag ctgcacgtat gaccaatgca agggcgtata ctgcggacaa
      601 taacgaaaaa acctggaagg gcaaaagagc cgcggtaaat acagaaggag gtgtaaccaa
      661 attcataagc gcatacaaga gcgaaacact aacgattatt gctactatct tacggttagc
      721 aaggcccgga aagtttgaac tgataaatcc cgaaaatccg cccacagcca gagaatacgt
      781 tagatagccc aatcgaaaca taagcgaggt ggataccccc gggaagatat cctgcaggag
      841 gaactcgtcg taacacaggc ttcgtagcgc aaagatacag gacaaaagcc caagccaaag
      901 gcaggaccgc tcctgacgat gcacagcaaa gagggcaaga aaatacgccc ccatcgcaag
      961 aatcgcccca aacggaataa tgatggaaat acgctttgta atataagcgt gtctgattgc
     1021 ccgggagggg ccgatgagta tggcggtttg agatgcggga aaaacatccg agtaattgga
     1081 aatatgaagg gtaaggacac acgaagaaag acccaaagga agcataacga cctggctatc
     1141 ccacccccac tgttcttcca caggattttc cgacggaagt ccacaagaat agatctgctt
     1201 cccgttgata aaatatcggc aagcgctact gtagccaggc aatcgtagag cataggagac
     1261 ggagggatcc agcccttcta tgcgaaccgc atagcttgca tgtcccttgc gcggaaatga
     1321 ttgggaatac tcgctccacg aggaggggaa gcgagcgaag gaggcggact caggtataga
     1381 gagctcgggt ggaataaacg taccaaacca aaaagcccag tctccttcca ggggaactgg
     1441 tgtggtatac ccgcgaagcg aagcacatgc attagtaact ggccacttct gcgcctgcac
     1501 acccgaaggc gaacaagaaa ctacaaaagt tgctatatag agaatcataa aaaaaaagat
     1561 taatcgtaga tacgaggggc gcaccttgtt gagtatgagg caacttgccc tgtttgtcaa
     1621 aaagaggcta cagtttgtac atgaagcgta tacagattat caccggagcc agctccggca
     1681 ttggacgaga attcgccttg cagatggcca gaaaccgtaa agccgacgag atatggctgg
     1741 tagccaggcg acgcgaccgg ctggaaagcc ttgctgcgga aattgccctt atagacgggg
     1801 cggcgaagcc ccttgttgta gagatagata tctccggcaa ggcgggagcg acaaaactgg
     1861 caaagacact tgagcgagag ggccagtcgg gagatcttac gatagacgcc ctgattaaca
     1921 atgcgggatt cggaacctat gggccttttg cggacacccc gcttgaaaag gaacttgaga
     1981 tgatagacct taacatcaca tctcttaccg ccctttgcca ccatgccctg ccctatcttg
     2041 caggcggctc tctggtggtt aatgtggcaa gccttgcggg ctttattccc ctggggaact
     2101 ttgccgtgta tgctgcaagc aaagcctacg tgttaagctt ctccgttgcc cttgcggccg
     2161 agcttgccga caggaagata ttcgtgtcta cggtatgccc gggcccggta gataccgagt
     2221 ttgccctggt tgcctcaagc ggtgcgcggg agaaggttgt ggacggaaag agtgcggagg
     2281 ccgtggtccg tgcctgcctt acggacgcac aacggggaaa acgtatttcc gtcatggcat
     2341 ggaaatggaa attcaaggca tttatgagcc gcttcgtggg cagatattgg tttgcacggc
     2401 acacctttgt gcacgaaaaa aggccgtcgc gaagcgacgg cgtctgatca gtcctcgaac
     2461 gacgtaaaga tttcctggat acggttgccg agttgtaagt acccggcagc cttgtctatc
     2521 ttcttgtcga agataagact catcctgagc gatccattag tcgtattcaa tccaataacc
     2581 gtttccccat taacggcggt aaacaccggt ccgtaaaaat cctgtaggac aaacggtcca
     2641 taagaaggag cgagtaccgt ccggcccaaa ttggaaaatg aaaaatcata atccatcgga
     2701 tcaggaggga agcccatcat aagggattca ggatctgcca cattgtcaaa ggaatcgcgg
     2761 aaatggaagt actcttcaac gtccttcatc ccttcccgtt gggcaagaac tcccgctcgt
     2821 attgtctcgg cgattacagc aaggtcttgg gaacagtcta ccggaacctt gacgattccg
     2881 ttatacaccc cgttcacgga acggtatttc tccttaaggt actgcctgaa atctaccggg
     2941 cactgaaagg ttcttgttgt gtttccctct gacgcaaaaa tatccgccga ggctttgagt
     3001 acccgcgcgc ctaaaaagct gttcaccgtc tgccccgcag cctttgcctc gctggccaat
     3061 cgggcggtac cggccggact gagggtaaag aaccgcagga aaaagtcggg tactgtgtat
     3121 gggggcatat caaaaggttc cggaggagga ggatcctctt ttttccagtc gggttcaggg
     3181 cgagtaagca gttctgccgt aacctcttcc ttaagagcct cgtataacgt gggcatattg
     3241 ggactggcag gcgcgtcaac ctggcctccg ccaagaacgg taagaaagtc gtggataaac
     3301 caggtggccg caataccgtc agcggctcca tgctgaaaca caaaaaagag ctcggct
//
LOCUS       DAOQXQ010000111         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29802_length_3353_cov_3.268041, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000111 DAOQXQ010000000
VERSION     DAOQXQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29802_length_3353_cov_3.268041"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..579
                     /locus_tag="PKH81_02625"
     CDS             55..579
                     /locus_tag="PKH81_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96962.1"
                     /translation="MASFRLVSTNGCAERRLCGSAARERHADVFRFIRLCIRFRLSHP
                     AFLRPEFLTGAERTSHSAPDITWFSSEGKSPDWSKLDRFVACRLIGTQAEILADRDDN
                     DFYLLFNASNRDVTATIPLPPGGKEWLRVIDTSRDSPEDILEPGREERFPGKDKYVVL
                     AQSVVVLISKILYS"
     gene            612..3062
                     /locus_tag="PKH81_02630"
     CDS             612..3062
                     /locus_tag="PKH81_02630"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNQ96963.1"
                     /translation="MDFDKEKFKASIRNRLKRHYGKNLEEANQHDLYDTVSTTVMDHI
                     QTNWMATRKKYEDDKSRQVYYLSAEFLMGRALGNNLINFGIMNKVREALDELEYDYNL
                     LEEEEPDAGLGNGGLGRLAACFLDSLATLEYPGHGYGIRYQYGMFEQRIENGYQVEHP
                     DNWLKHRDPWEVKRSDRAVKVLFGGTPITEKGPKGNVVFKLINAEEILATPYDIPIVG
                     FDTRTVNRLRLWEATSENGFDLQLFNDMHYIRAVERQNNAENISRVLYPNDSGPSGKA
                     LRLKQQYFFTSASLQDLVRKFKRDHGSDFSRFPTYNVIQLNDTHPVVAIPEMMRLLMD
                     ENGLGWDDAWDIVSRTFAYTNHTILAEALEKWPIDIFQGLLPRVYQIVEEINRRSLSV
                     IREKFPGDWKRQNRMGILGDGMIRMASLAIVGCFSVNGVAALHTQILKESALSDWYEL
                     FPSKFNNKTNGVTQRRWLMLSNPRLSAFITARIGSGWEKDLSRLKALEAFADDEASLD
                     EFMDIKRNNKVDLAKYLKDSQNIFIDPDSIFDVQVKRLHEYKRQLLNILHVMYLYNRL
                     IADPSAVMVPQTFIFGAKAASGYRRAKSIIKLITSVADRINNDHRVKGRLRVVFVENY
                     RVSLAEHLFPASDISEQISTAGKEASGTGNMKFMMNGALTIGTLDGANIEIVEEAGAD
                     NAFIFGLTADEIRKIESEHSYNPQEYLAANPELALVVNQLIDGTYSSMSDNLFRELYD
                     SLVYGVDGQRPDVYYVLADFEAYALAHQNMADAYSDRKKWARMALLNVARSGKFSSDR
                     TIEDYAREIWKINKVVVD"
     gene            3080..>3353
                     /locus_tag="PKH81_02635"
     CDS             3080..>3353
                     /locus_tag="PKH81_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96964.1"
                     /translation="MKKCIALCVCLTLALCAISAQAPKKILSNGDIDAFVKNMPTIDA
                     ELSALGDKYNDFFEPAMSESASPSENMATMRAINPPDEIKAILKKNG"
BASE COUNT          840 a          805 c          877 g          831 t
ORIGIN      
        1 ctggaagtcg ccccgatccg ccaagagcat acctgctgtc ggagggttct tcggatggcg
       61 tcgttccgtc tcgtctcgac gaacggttgc gccgagcggc gcctctgcgg tagcgcggcc
      121 cgagagcggc atgccgatgt attccggttt attcgcctgt gtattcgctt tcgcctgtct
      181 catcccgcgt ttttgcgtcc cgaattcctg accggcgcgg aacggacaag ccactcggct
      241 cccgatatta cctggtttag cagcgaggga aaaagcccgg actggtccaa gcttgaccga
      301 tttgtcgcgt gtcggctcat cggcactcag gcagagatac tggcagatcg ggacgataat
      361 gacttttatt tactctttaa tgcttcgaat cgcgatgtaa cggcaactat tccactgcct
      421 cccggaggca aagaatggct tagggtgata gatacatccc gggacagtcc cgaagatatt
      481 ctggaaccgg gcagggaaga gcgatttccg ggaaaggaca agtacgtggt gcttgcccag
      541 agtgtggttg tactgatttc aaagatatta tacagttaat taaccggttt acctgaagga
      601 gaaaacgcac catggacttc gacaaggaaa aattcaaggc ctcgatacga aacaggctta
      661 agcggcatta cggaaaaaac ctggaagaag cgaatcagca tgatctatat gatacggttt
      721 cgacgacggt tatggatcac attcaaacca actggatggc tacccgcaag aaatatgagg
      781 atgataagtc ccgccaggtg tactacctgt cggccgagtt tttaatgggt cgcgccctgg
      841 ggaataacct gattaacttc gggattatga acaaggttcg cgaagccctg gatgaacttg
      901 aatatgatta caacttgctt gaggaagaag aacccgatgc cggacttggc aacggtggcc
      961 ttggccggct cgccgcctgt tttcttgatt ctcttgcaac ccttgagtat ccgggccatg
     1021 gttatggcat ccgctatcaa tatggcatgt tcgagcaacg catcgaaaac ggctatcagg
     1081 tagagcaccc cgataattgg cttaagcatc gagatccctg ggaggttaaa cgatccgacc
     1141 gggcggttaa ggtattgttt ggtggaacgc ctatcaccga aaaaggaccc aaggggaacg
     1201 tggtctttaa gctcatcaat gccgaggaaa ttcttgccac tccctacgac atcccgatag
     1261 tcggctttga tactcgcacc gtgaatcgac tgaggctctg ggaggcaact tccgagaacg
     1321 gctttgatct tcagctgttt aacgacatgc actacatccg cgccgtagag cggcaaaaca
     1381 acgccgagaa catctcccgc gttttgtatc ccaacgattc aggtccgtcc ggaaaggcgc
     1441 ttcgtctaaa acagcaatac ttctttacct ctgcaagtct tcaggatctg gtgcgcaaat
     1501 tcaaacggga ccatgggtcg gatttttccc ggttccccac ctacaatgtg attcagctta
     1561 acgacaccca tccggttgtc gccattcccg agatgatgcg gcttttgatg gacgagaatg
     1621 gtcttggatg ggacgatgcc tgggatattg tgagccgaac ctttgcgtat accaaccaca
     1681 cgattcttgc cgaggccctg gaaaagtggc ccatcgatat ctttcagggg ctccttcccc
     1741 gtgtgtacca aattgtggaa gagataaatc ggcgttccct ttcggtgatt cgggagaaat
     1801 tccccggaga ctggaagcgg caaaaccgta tgggaatact cggagacggc atgattcgca
     1861 tggcatctct tgcgattgtc ggctgttttt cggttaacgg agttgcggcc ctgcatacac
     1921 aaattctaaa ggaaagcgcc ctttcggact ggtacgagtt gttcccttca aaattcaaca
     1981 acaagacaaa cggggttacc caacgccgtt ggcttatgct ctctaatccc cggctttccg
     2041 cctttattac cgctcgcatt ggatcgggtt gggaaaagga tttgtccagg cttaaggcgc
     2101 ttgaggcctt tgcagatgat gaggcttcgc ttgatgagtt tatggacatt aaacgaaaca
     2161 acaaggttga tttggccaag taccttaagg attcccaaaa tatctttatc gaccctgatt
     2221 caatctttga tgtacaggtt aaacgtctgc atgagtacaa gcggcaactt ttgaacattc
     2281 tgcatgttat gtatttgtat aatcgcctta ttgccgatcc ctctgcggta atggtaccgc
     2341 aaacctttat ctttggagcc aaggcggctt cgggatatcg aagggcaaag agcattatca
     2401 agttgattac ctcagtcgct gaccgaatca ataacgatca tcgggttaag ggtcggctca
     2461 gggttgtgtt tgtggaaaat taccgggtgt cgcttgcgga gcatctgttt cccgcatcgg
     2521 atatctctga acaaatttcc acggcgggta aagaagcttc cggtaccggt aacatgaagt
     2581 ttatgatgaa cggagctctg acgattggaa cccttgatgg tgcgaatatc gagattgtgg
     2641 aagaagcagg cgcggacaat gcctttatct ttggcttaac cgccgatgaa attcgcaaga
     2701 ttgaaagtga gcacagttac aatcctcaag aatatcttgc cgccaatccg gagctggcct
     2761 tggtagtaaa ccagctcatt gatggaacat acagctcaat gtctgacaac ttgttccggg
     2821 agctttacga ttccctggta tacggcgtgg acggtcagcg accggacgtg tattatgtgc
     2881 ttgccgattt tgaggcctat gccctggcgc atcagaacat ggccgatgca tatagcgacc
     2941 gaaagaaatg ggcacggatg gcgcttctta atgtggctcg atccggtaaa ttcagttctg
     3001 accggaccat agaagactat gcccgcgaga tatggaaaat taacaaggtt gtggtagact
     3061 aaaccaccaa ggagctatta tgaagaagtg tatcgcgcta tgcgtttgtt tgacccttgc
     3121 tttgtgtgca atttctgcgc aggcgccaaa aaagatattg agcaatggtg atatcgacgc
     3181 ctttgtaaaa aatatgccaa cgatcgacgc tgagcttagc gccttgggcg acaagtataa
     3241 cgactttttt gagcctgcta tgagcgagag cgccagcccg tccgaaaaca tggcgacgat
     3301 gcgcgctatc aatcctccgg acgagattaa ggcaattctc aaaaaaaacg ggc
//
LOCUS       DAOQXQ010000112         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_29820_length_3352_cov_2.961177, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000112 DAOQXQ010000000
VERSION     DAOQXQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29820_length_3352_cov_2.961177"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1629
                     /locus_tag="PKH81_02640"
     CDS             64..1629
                     /locus_tag="PKH81_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ96965.1"
                     /translation="MSDGNLVPAGKQTPSFEEFKKFVLQYGAEKDFLGRIITLHGKIP
                     RLIRVLFNFDKTGFTSAIDQLINEEKSEREQDNILRAIYTLGIRIWNPESECMEATAL
                     QSLQFARLKNLVERVYTNVPYYRKKFDEMGLTPSDIKSLSDIAKLPFTTKDDLRETYP
                     YGLLAVPQAEIVEIHMSSGTTGVPVVDAYTKRDMDDWAEGMARTLSGAGATRSDTVQN
                     AYGYGLFTGGLGTHYGAQRIGATIIPISSGNTQKQLMLMRDFKSTIFTCTPSYALYMA
                     EQAHEMGIDPSTLGIRSGCFGAEPWSENMRKEIEKAWHIKAYDIYGLTEITGPGVAFE
                     CEGQYGMHVNEDLWFPEIIDPATGKSLPDGEKGELVITTITKEGTPLIRYRTRDITFI
                     IPETCGCGRTTRRIHRLFGRTDDLLIIRGVNVFPSQIEHALIEIQGVDPNYLIIVDRS
                     AQTHLDDVELHVEVNPAAFSDETKDMESLRNRIEATMKSKLGITLKVKLVEPKSIERS
                     IGKAKRVIDKRQI"
     gene            1644..2060
                     /locus_tag="PKH81_02645"
     CDS             1644..2060
                     /locus_tag="PKH81_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNQ96966.1"
                     /translation="MKIQQISIFLENAAGRLAEVTRELKDGGVNLRAIMIADTADFGI
                     LRVIVDDPAKAISILEKAQFTTKTTDVLAVTITDKVGSLADVLELFEKNGVNIEYLYA
                     ALNKAGETAVIIFKVEDIALGLDMVNKHGLYKDSPF"
     gene            2073..3077
                     /locus_tag="PKH81_02650"
     CDS             2073..3077
                     /locus_tag="PKH81_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ96967.1"
                     /translation="MAVSIRDVAKVASVSCESILEALISPSSLEPEVVRFVMQTIRTS
                     GFLETFSHWKKGEQRSAIAVAVPSLESPYYVEIFRGIDRALSSLGLKTATIVFPTRNS
                     PVFLEEVLFSTLHNPVVSAVIVISQSPSLGIVEKFEAEKKPLILLQSTAQGAQSVLLE
                     NQKGMSIAVNHLFHKGKQRIALINTPTSGRDSTSVASERLMGYVTALHRNALDFDENL
                     VFEAADWDGNDGIAAFDYFSSFESMPDAVICASGDMNAMGFINAARSASLRIPEDIAV
                     MGYGDLKIASLMHPPLTTIRQRLMIAGAGALVLALESCINGRGENLIIMPELVERETV
                     "
     gene            3126..>3352
                     /locus_tag="PKH81_02655"
     CDS             3126..>3352
                     /locus_tag="PKH81_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ96968.1"
                     /translation="MDNTRAIHWADQTADKIIRERGDLPLYTCASGITPSGTVHIGNF
                     REIISVDLVVRALRARGKQVRFIYSWDDYDV"
BASE COUNT          864 a          797 c          832 g          859 t
ORIGIN      
        1 gtacacgatt tgtttcttta ctgagttatg ctacaatctt ttagcaaaat ggaatcatct
       61 catatgtctg acggtaacct agtcccagct ggaaaacaaa ctccatcttt tgaggagttt
      121 aaaaagttcg ttcttcaata tggcgcggaa aaagattttc tcggtcgaat aattactttg
      181 cacggcaaga ttccaagatt aatcagagta ctatttaatt tcgataaaac aggatttact
      241 agtgcgattg accagctaat caatgaggaa aaatctgagc gagaacaaga caatattctt
      301 cgtgcaattt acactttggg gattagaatt tggaacccag aaagcgaatg catggaagcg
      361 actgccctgc agtcattgca gtttgcccgc ctcaagaatc ttgtggagcg ggtctataca
      421 aacgttccgt attaccggaa aaaatttgac gagatgggcc ttacgccatc cgacataaaa
      481 agtctgtcgg atatcgccaa gctccctttt accaccaagg atgatttacg cgagacttat
      541 ccgtatgggc tccttgccgt tccccaagcg gagattgtcg agattcacat gtccagcggt
      601 acaaccggcg tgccggtcgt ggatgcgtat accaaacgcg atatggatga ctgggccgag
      661 ggtatggctc gcaccctttc gggcgccggc gcaactcgta gcgatactgt tcaaaatgcg
      721 tatggctatg gcttatttac cggaggcctg gggactcatt acggcgccca gcgcattggc
      781 gcaactatta ttccaatatc ctccggcaat acgcaaaagc agctcatgct gatgcgcgat
      841 tttaagtcta ccatttttac ctgtactcct tcatatgccc tgtacatggc agaacaggct
      901 catgaaatgg gcattgatcc gtcaacgctc ggtatccgtt ctggctgttt tggggccgag
      961 ccctggagcg aaaacatgcg taaggaaatc gaaaaagcct ggcatataaa ggcttacgat
     1021 atttacggcc ttaccgagat taccggccct ggcgttgcct ttgaatgcga agggcagtat
     1081 ggcatgcacg taaacgagga cctgtggttc ccggaaatca ttgatcctgc aacgggtaaa
     1141 agtcttcccg acggcgaaaa gggagagctg gtaataacga cgattaccaa ggaaggcacc
     1201 ccgcttattc gctatcgaac acgggatatt acctttatta ttcccgagac ctgtggttgt
     1261 ggacgaacaa cccggcgaat acaccgcctc tttggccgca ccgacgatct attgattatt
     1321 cgcggcgtaa atgtcttccc cagtcagata gaacatgccc ttatcgagat acagggcgta
     1381 gacccgaatt atctgattat cgttgatcgt agtgctcaga ctcatcttga cgatgttgaa
     1441 ttgcatgtag aggtcaatcc ggcagctttc tcggacgaga caaaggacat ggaatctttg
     1501 cgaaatcgca tagaggctac catgaagagc aagttgggta ttacacttaa ggttaagctg
     1561 gttgaaccaa agagcatcga acgttcaatc ggaaaagcca agcgggtaat cgacaaacgt
     1621 caaatctaga aagggggaac cggatgaaga tacaacagat ttcgattttt ttggaaaacg
     1681 cggccggtcg gcttgccgag gttacgcgag aacttaaaga cggcggggtt aatctcaggg
     1741 caatcatgat tgccgacacg gcagattttg gcatccttag agttattgtc gacgatccgg
     1801 ccaaggctat atcaatactg gaaaaagcgc agtttaccac caagaccacc gatgtactgg
     1861 cggtaactat taccgacaag gtaggcagtc ttgcggacgt gcttgagtta ttcgaaaaaa
     1921 acggggtcaa catagaatac ctgtatgccg cccttaataa ggcgggagag accgcagtta
     1981 ttatttttaa ggtagaggat atagcccttg gccttgatat ggtaaacaag cacggtctgt
     2041 ataaagactc accgttttaa ggaagtgtaa tcgtggctgt ttctatacgg gatgtcgcca
     2101 aggtcgcctc ggtgtcctgc gaaagcatac ttgaagcgct tataagtccg tcctctcttg
     2161 agccggaggt tgttcggttt gttatgcaaa ccatcagaac ctccgggttt ctggagacct
     2221 tttctcactg gaaaaaagga gagcaacggt cagccatcgc tgtcgctgtt ccttctcttg
     2281 aatcccccta ctatgttgaa atattcaggg ggattgaccg ggccttgtct tcccttgggc
     2341 ttaaaacagc tacgatcgtc tttccaacca gaaactcacc ggtgtttttg gaagaagtcc
     2401 tttttagtac cctgcacaat ccggttgtct cggcagttat cgtcataagt cagtctcctt
     2461 ccctgggtat tgtagaaaaa ttcgaggcag aaaagaaacc gttgatcctg ttacagtcaa
     2521 cggctcaggg ggctcagtcg gtattactgg aaaatcaaaa gggaatgtca atcgcggtaa
     2581 atcatctgtt tcataagggt aagcagagaa tcgctcttat caatactcct acttcgggta
     2641 gggacagcac ctcggtcgct tcggagcgcc tgatgggata tgtaaccgcc ctgcaccgta
     2701 acgcccttga ttttgacgaa aaccttgtgt ttgaagcggc agactgggac ggaaacgacg
     2761 ggatagcagc ctttgactat ttttcttctt tcgagagtat gcccgacgcg gttatctgcg
     2821 cctcgggtga tatgaatgcc atgggcttta taaatgctgc tcgttccgct tcacttcgga
     2881 ttcccgagga tatcgcggtt atgggctatg gggatcttaa aatcgcctca ctaatgcatc
     2941 cccccctaac cacaatccgt caacgactga tgatagccgg cgctggagct ctcgttctgg
     3001 cccttgagtc ctgtatcaac ggacggggcg agaatttgat tattatgccc gagcttgtgg
     3061 agcgcgaaac ggtatagaca gcccgattgt cctcctgtgg tgaattcgct atacttatcg
     3121 ctattatgga caacacgaga gcaattcact gggcggatca aaccgccgac aagattatcc
     3181 gcgagcgcgg cgaccttcca ttgtatacct gcgcttccgg catcacccct tccggtaccg
     3241 ttcatatcgg aaatttccgc gagattatct cggtggacct tgtcgtccgc gcccttcgcg
     3301 cccgaggcaa acaggtacgc tttatctact catgggatga ttacgacgta tt
//
LOCUS       DAOQXQ010000113         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_30292_length_3316_cov_2.848513, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000113 DAOQXQ010000000
VERSION     DAOQXQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30292_length_3316_cov_2.848513"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKH81_02660"
     CDS             complement(<1..993)
                     /locus_tag="PKH81_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96969.1"
                     /translation="MSMLIFSYILAGFLFAFSYYLDSRTEGNTGSFTGMTMVSAFIAL
                     LAGLSANLIYTGNLTISLLLFRFSLVCFSILSTFLFRYALSIPYNTSNKFIRLLGWLA
                     VAGAGYMVFTSIKAVGITQEGFFTISSVPFFGIANGFPLFVTVYLVGIPALTVLILFF
                     RAAGIRSRIFRQRLIFVAVSICSGFAVSYFLFRLSASFPWALPLVPFGLAVMLVLVKQ
                     AISVTTLYDRSLATATIINFVIINGAFSALTGLVAAAILTSVSDIRLALVFVVLVTIC
                     MLFLREVVFTRLRKYIRVGVDYEADLEAALDSLDYTSGGQVVIEKAVGLLSQYLE"
     gene            complement(996..2378)
                     /locus_tag="PKH81_02665"
     CDS             complement(996..2378)
                     /locus_tag="PKH81_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ96970.1"
                     /translation="MIQSRKRLAKLAIDALVGLVFSFFMFIILPQFSFTGRFISAAVV
                     FFVVATSSSLLVKEVWQRLEFQAFKVRDSRLMATFIERLRFSYTIEDLFESIQTVLEH
                     EADASVLYVNSENSYVMYNSPTRIATDPDTLEVLSRNFPANWPEGFYLIDEELGLVSD
                     FYNARGFFLVYDKLHFYVMCRYMKVFEKSIFDTMMYEFANFQKRTRTITQLTAISELS
                     KEWDMVAQTQVSFLPQSMPNIPKLDIAAYFRPLVNVSGDFYDIIELDKDRTFFLLGDV
                     SGKGLASALIMGVIMNTVKIIENKEDLPGVVRAIDTAIKSMRLQDKYAVLFICIIDTR
                     VMKIRYVNASMADPIIITKAPGGYKIKPLQSTCSLVGIIDLDEVIQEEAPLYRGDVVL
                     MASDGVSEVMDESGVELGDTELYLNTVKNCAHKSAKHFINDIADLVLEYNGDKKLRDD
                     VTMLVAKIEE"
     gene            complement(2569..>3316)
                     /locus_tag="PKH81_02670"
     CDS             complement(2569..>3316)
                     /locus_tag="PKH81_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNQ96971.1"
                     /translation="LRYDLQISFQDAVYGTKAEIQYNRNEACSACKGSGASGGSGRKM
                     CATCQGAGQVRHSSGFFSIASPCPTCHGEGTIIENPCRECSGSGLQKKRQKILVTIPA
                     GVEDGKRISIPRQGDAGPNGGPYGDLFVFIRVKAHAWFERSGSDLYCAIPISMTQAAL
                     GAEIQIAALDGKQLKLKIPAGTQNDKLLRIRDEGVPVANGRKGDLYIKVIVRIPSKLS
                     AKAKQLLEEVSRMEGENATPSPIPLADLRS"
BASE COUNT         1028 a          781 c          792 g          715 t
ORIGIN      
        1 ttcaagatac tgagatagaa gaccaacagc cttttcaata accacctggc caccggaggt
       61 atagtcaagg gaatcaagag ccgcttccag atcggcctca taatccacgc caacacgaat
      121 atactttcgc aagcgagtaa aaaccacttc cctaagaaac agcatacaaa tggtaacaag
      181 gactacgaac accagcgcaa gacgtatgtc ggacacagaa gtcaatatag ctgctgcaac
      241 caaaccggta agcgcactaa aggcgccatt gattataaca aagttgataa tagtagcagt
      301 ggccaaagaa cgatcataca gcgtagtaac cgaaattgcc tgtttgacca atacaagcat
      361 taccgccaag ccaaagggaa ccagaggcaa ggcccagggg aaagaggcgg atagtctaaa
      421 aaggaaataa gagacagcaa agccgctaca aatagacaca gcaacaaaga taagacgctg
      481 acggaagata cggcttctaa taccggcagc cctgaagaac aagatcagaa ccgtaagggc
      541 agggattccc accaaataca cagtaacaaa aagagggaag ccattggcaa tgccaaaaaa
      601 ggggaccgaa gaaatggtaa aaaaaccttc ctgtgttata ccaacggctt taatcgacgt
      661 aaacaccata tacccggcac cggcgacagc cagccagcca agcaagcgga taaacttgtt
      721 tgaagtatta tagggaatcg ataaggcata ccggaataaa aaagtggaca ggatagaaaa
      781 gcagaccaga gaaaaccgga aaagcaagag actgatggtc agattgccgg tatagatcaa
      841 gttggccgaa agacccgcaa gcagggcgat aaaggccgac accatagtca tgccggtaaa
      901 cgatccggta ttaccctcgg tacgcgaatc cagataatac gaaaaggcga agagaaaacc
      961 agccagaata taactaaaga tgagcatgct catgattact cctcgatctt tgcgacaagc
     1021 atggtaacgt cgtcgcgaag tttcttgtcg ccgttgtatt ccagaacaag atcggcgatg
     1081 tcattgataa aatgcttggc acttttgtgc gcgcaatttt taaccgtatt caaataaagc
     1141 tcggtgtccc caagttccac accggattca tccataacct cggatacacc gtcagaagcc
     1201 ataagaacca catctccgcg gtacaaagga gcttcttcct gaattacctc gtcaagatcg
     1261 ataataccaa ccagggagca ggtagactga aggggtttaa tcttgtatcc gccaggcgcc
     1321 tttgtgatga taattggatc agccattgag gcgtttacat agcgaatctt cataacccga
     1381 gtatcgataa tacagataaa aaggactgca tacttatcct gaagccgcat cgacttgatc
     1441 gcggtatcaa tggctctgac aacgcccgga agatcctctt tattttcgat aatcttaacg
     1501 gtattcatga taacgcccat aataagcgcg gaagcaagac cctttccgga gacgtcccca
     1561 agcaggaaaa aggttctgtc tttatccagc tcgataatgt cgtaaaagtc acctgatacg
     1621 ttaaccaggg ggcgaaaata agctgcgata tcgagcttgg gaatattggg catactttgc
     1681 ggaagaaagg aaacttgcgt ttgagcaacc atgtcccatt ccttggaaag ttcggaaatg
     1741 gcggtaagtt gggttattgt gcgcgttcgc ttttggaaat tcgcgaattc gtacatcatg
     1801 gtgtcgaaaa tacttttttc gaataccttc atgtacctgc acatcacgta gaagtgaagc
     1861 ttgtcgtaca ccaggaaaaa tccgcgggca ttataaaagt cggatacaag accgagttct
     1921 tcgtcaatta agtaaaaacc ttctggccag tttgccggaa agttacggga cagcacttcc
     1981 agtgtatccg ggtctgttgc aatgcgggtg gggctattat acataacata ggagttttcg
     2041 gagtttacat acaacaccga ggcatcggcc tcatgctcca gaacagtttg tatagactca
     2101 aacagatcct caatggtgta ggaaaaccgg agccgttcga taaatgttgc cataaggcga
     2161 ctgtcgcgta ccttaaaagc ctgaaactcc agacgctgcc acacctcttt taccaacaac
     2221 gaagaagagg tggcaacaac aaaaaaaaca accgcggcgg aaatgaacct gccggtaaaa
     2281 gaaaactgcg gcaaaataat aaacatgaaa aaggaaaaaa ccagaccgac caaggcatca
     2341 atggcgagct tggctagtcg ttttctggat tgaatcatgc tgatgcacct ctttgaagaa
     2401 gagaaggtat ccggaaaggc attagattcc ggaaaaacgc ccgtacgtat cgatgtgaca
     2461 taaccggcac actatattct atcatataaa ggaacatttg agaataaaaa tttggacagg
     2521 gagcgctgta gctcaatacc ccggggataa tcccggggta tgtatcagtt aagagcgtaa
     2581 atctgcaagc ggaatgggac tgggagttgc gttttcgcct tccatgcggg aaacttcttc
     2641 caacagctgt ttagccttgg cagaaagctt ggaaggaatg cgaacgatca ccttgatata
     2701 cagatctccc ttacggccat tggcaacagg aaccccttca tcccggattc gcaagagctt
     2761 gtcgttttga gttcctgcgg gaatcttgag tttaagctgt ttgccatcaa gggccgctat
     2821 ctgaatctcg gcgccaagag cggcctgggt catggaaatg ggtattgcac aatagagatc
     2881 tgaacctgat cgttcaaacc aggcatgcgc tttaacgcga ataaagacaa ataaatcgcc
     2941 atacgggccg ccgttaggac ctgcatcgcc ctgacggggt atagaaatac gtttgccgtc
     3001 ctctacaccg gcgggtattg ttaccagaat cttttgacgc ttcttctgga ggccgcttcc
     3061 cgaacattcc cggcaagggt tctcgataat ggtaccctcc ccatgacagg tagggcacgg
     3121 ggatgcaatg gagaaaaagc ctgaactgtg acgcacctgg ccggcgccct gacaggtagc
     3181 gcacatcttg cgtccgcttc cccccgaggc cccgcttccc ttacacgctg aacaggcttc
     3241 gttgcggtta tattggatct ctgccttggt tccgtagacc gcgtcctgga aactaatctg
     3301 aagatcgtag cggaga
//
LOCUS       DAOQXQ010000114         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_30451_length_3304_cov_2.415820, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000114 DAOQXQ010000000
VERSION     DAOQXQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30451_length_3304_cov_2.415820"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PKH81_02675"
     CDS             complement(<1..1414)
                     /locus_tag="PKH81_02675"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HNQ96972.1"
                     /translation="MSTTRNQNDEFHFSCDSDTAQPQRSGTWKILIVDDDESVHLITK
                     MILGNFSFQNKTLDIQSAYTEEDACTLIRENPDFAVILLDVVMSREDSGLRIAKYIRD
                     ELQNKMTRIVLRTGQPGQAPEKKVILDYDINDYKLKTELTADKLFVTMVSALRGFSDL
                     LTIEYNRKGLEKIIDSSSSLFKPQSFSEFVSGVLIQMTSLMHLGQSALYCRTSGISAR
                     KTENHFIVEAGIGDFTQSVQLPLEEALSQETLAIVEKAIANKQSLYLDKSFIAYFKGA
                     GGSENIIYMESTNSISEWDKNLIGIFCTNISVALDNMTLNQEIENTQKEIIYTLGEVA
                     EARSKETGHHVKRVAEYSKLLALTIGLDEKQAETIRLATPMHDIGKLAVPDAILNKNG
                     PLTADEFDIIKTHSMAGYDMLKNSNREIMRVAALIALEHQEKYDGSGYPFGKKGDDIS
                     LEGRIVAIADVFDALGCDRVY"
     gene            complement(1398..2264)
                     /locus_tag="PKH81_02680"
     CDS             complement(1398..2264)
                     /locus_tag="PKH81_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ96973.1"
                     /translation="MLVLLASISTLISLVVSVKLWIVYRKAQRMHRMLITSETMASMG
                     KLVAPVTHDLNTNLGVCISAVSYLTDIIEETKKNVTQGTLTKTGFEQQLHTEREAADL
                     VMLNLEKTVELVSGLKRMSIDQETNAERTFNLEEYLNQIVTGLHPNIRKTGHKIEIDC
                     PRSIVITVKAGALTRVLNNLISNALLHAFTDNEKGLIVISAKEINSEVRISFRDNGRG
                     MSESVLSRAFTPFFTTRGASGGSGLGLSIVKNLVKKELQGSIECTSEIGKGTEFIITI
                     PREISKHNEYNA"
     gene            2375..>3304
                     /locus_tag="PKH81_02685"
     CDS             2375..>3304
                     /locus_tag="PKH81_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ96974.1"
                     /translation="MKKRNPFWRCTALFVCACVFIACAPKDTNSSKKTVEVSWWHINS
                     SIRSHQTFQDIVADYHLLYPGVTISVTALENMEYKPKLELEFAADDPPDIFHSWGGGG
                     LAEYVRKGYLRDITDWVLSDKWESKINPAALELFSYKGRVYGFPHDLGAVGFWYNSAL
                     LEGAGYREFPSDWDSLLSLMEALKKKGVTPVSLGIADRWPVMYYWVYLSMRIGGDDIF
                     KDILARKRAFTDPAIITAGKLLQDFNRRGFFQSTAIGDDFHGQSRYMGDGLCAIQLMG
                     QWALASQAQTSERTNELHPVMRFAPFPSVQGGKG"
BASE COUNT          798 a          708 c          777 g         1021 t
ORIGIN      
        1 tatacaccct gtcgcagcca agggcgtcaa aaacatcggc tattgccaca atcctgccct
       61 ccagactgat gtcatcaccc tttttaccaa atgggtatcc cgaaccgtcg tatttttctt
      121 gatgttcaag ggcaataaga gccgccaccc gcataatttc ccgattcgag tttttaagca
      181 tgtcgtatcc ggccatcgaa tgggttttga ttatgtcaaa ttcgtccgca gtgagtgggc
      241 cgtttttgtt gagtatcgcg tcgggaacag cgagcttacc aatatcgtgc attggggttg
      301 ccagccttat ggtttcggct tgtttttcat cgagcccaat ggtaagggca agcagttttg
      361 aatactctgc aacccgcttt acgtggtgtc ctgtttcctt tgaacgtgcc tcggcaacct
      421 ctcccagggt gtaaatgatt tctttttggg tgttttctat ttcctggttc agtgtcatgt
      481 tatcaagagc tacagagatg tttgtgcaaa aaataccaat caggttctta tcccattcgc
      541 taattgaatt ggtgctttcc atgtaaatga tattctcact gccacccgct cccttaaaat
      601 aggcaataaa acttttatca agatacaagc tttgtttgtt ggctatcgct ttttccacga
      661 ttgcaagcgt ttcctgggag agcgcttctt ccagggggag ttgtacgctt tgagtaaagt
      721 cgccgattcc cgcttctaca ataaaatgat tctccgtttt gcgcgcagat attccggatg
      781 ttctgcaata cagtgcgctt tgtccaagat gcataaggga tgtcatctgt atcaaaacac
      841 ccgacacaaa ttcagaaaag gattgaggct taaaaagact tgaggatgag tcaattatct
      901 tttccagacc cttgcgattg tattcgatag ttaataaatc actaaagccc cgcagagcgg
      961 aaaccattgt tacaaacagc ttgtccgcgg taagctccgt tttaagtttg taatcgttga
     1021 tgtcatagtc aagaataact tttttttctg gggcttgacc gggttgtcca gttcttagga
     1081 ctatgcgagt cattttgttt tgtagctcat cccgtatgta tttggctatc cgtaacccgc
     1141 tgtcttctct gctcattacc acatcaagaa ggattaccgc gaaatcagga ttttctctta
     1201 tcaaggtgca ggcatcttct tcggtatacg cgctttgtat atcgagcgtt ttattttgaa
     1261 aagaaaaatt gccaagaatc attttggtaa taagatgaac ggattcatca tcgtctacaa
     1321 tcaaaatttt ccatgtgccg gatcgctgag gttgagccgt atcactgtcg caggaaaaat
     1381 ggaattcatc gttttgatta cgcgttgtac tcattgtgtt tactgatctc ccggggtatg
     1441 gtgataatga actcggtgcc tttgccaatc tcgcttgtgc actctatgct tccctgtaat
     1501 tcctttttaa ccaaattctt tacaatactc aagccaagac cgcttccgcc agaggcgccc
     1561 ctggtggtaa aaaaaggcgt aaaggctcgc gagagtacgc tctcgctcat cccccgccca
     1621 ttgtcgcgga aactgatacg tacttctgag ttgatttctt tagcgctgat gacgattaat
     1681 cccttttcat tatcggtaaa agcatggagg agtgcgttgg aaatcaggtt attaagaacc
     1741 cgggttaagg ctcctgcttt tacagttatc acaatagagc gcgggcagtc aatttcaatt
     1801 ttgtggcctg ttttgcgaat gtttggatgt aagcccgtta ctatctggtt aagatattct
     1861 tcaagattaa aggtacgttc tgcattcgtt tcttggtcta tagacattcg ctttaagccg
     1921 gaaaccagtt ctactgtttt ttccagattg agcatgacaa ggtctgctgc ttcccgttcg
     1981 gtatgtaatt gttgctcaaa tcccgttttt gttaaggttc cttgcgtaac gttttttttt
     2041 gtttcttcga taatatctgt aagatacgag acggcagaaa tacagacacc caaattggtg
     2101 tttaaatcat gagtaaccgg tgcgaccagt ttacccatag atgccattgt ttcgcttgta
     2161 atgagcatgc gatgcatgcg ttgcgccttt cggtatacta tccacagctt gaccgacact
     2221 actaacgaga taagtgtgct gatactggcc agtagaacaa gcatagctat agtgtggttc
     2281 ccattttcca ggctgtcaag ttttccctgg caatcttggg cttctcggcg tatgattcac
     2341 ctatggcata ttcaaaaccc tagggaggtg cattatgaag aaaaggaatc cattctggag
     2401 atgtaccgct ttgtttgtgt gcgcgtgtgt ctttattgcc tgtgcgccaa aggatactaa
     2461 tagttcaaaa aaaacggtag aggtaagctg gtggcatatt aattcttcca ttcgctctca
     2521 ccaaaccttt caggatattg tcgcagacta tcatctattg tacccggggg taactatcag
     2581 cgttactgcc ctggagaaca tggagtataa gccaaaactt gagcttgagt ttgccgcaga
     2641 tgatccccct gatatttttc atagttgggg agggggagga cttgcagagt atgttcgcaa
     2701 gggctatctg cgagacatta ccgattgggt gttaagcgac aagtgggaga gcaagattaa
     2761 tcctgctgct ttggagctgt ttagctataa gggtcgggtg tatggatttc ctcatgatct
     2821 tggtgcggtg ggattttggt ataacagcgc gcttttggaa ggagccggtt atcgtgaatt
     2881 cccctccgac tgggattcgt tactctcttt gatggaagcc ttaaagaaaa aaggggtgac
     2941 ccctgtatct ctgggtatcg cggaccgctg gccggtaatg tattactggg tgtacctttc
     3001 catgagaata ggtggagatg atatttttaa ggatattctg gcaagaaaga gagcctttac
     3061 cgatccagcc ataattaccg ctggaaagtt attgcaggat tttaacaggc gaggtttttt
     3121 tcagtcaacc gctattggag atgattttca tggccaatcc aggtacatgg gagatggtct
     3181 ttgcgcgatt cagttgatgg ggcaatgggc tttggcatcg caagcgcaaa cctcagagcg
     3241 gacgaatgag ctgcatccgg taatgcgctt tgcgcccttt ccctctgtgc aaggagggaa
     3301 gggt
//
LOCUS       DAOQXQ010000115         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_30552_length_3296_cov_4.216291, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000115 DAOQXQ010000000
VERSION     DAOQXQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30552_length_3296_cov_4.216291"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKH81_02690"
     CDS             <1..1028
                     /locus_tag="PKH81_02690"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNQ96975.1"
                     /translation="KHNCRFASYTLLTKGSYPAELDIRLPFSMVLENRKTDRLEVMPA
                     YYWMYNMYALERNSWKYRTRDKRKNIVQRIETDHLAPDTAREMIEGMRLLEIWTGKAW
                     YSSESAQGSMPSEEVLAEKGRDLITREPEIVKSLFVSGEAMERGNREVRILKVVQAWN
                     AYRQMLLFYGIKTIASYLDEYNIHYEYFSAQVPQSISLEWVNLGGQLVPEPKVDGLRS
                     AVRTGLFSTWKEIHEQYESWWTLYPRDKAENAYAILRLLCKCDSISPERWNALLEEAK
                     GIRTYIEEQVYITKKKDFENSYRMITYRNTAERDAVLGTVDDNGFIRTAKKESAEFFA
                     LLERVRG"
     gene            1031..1915
                     /locus_tag="PKH81_02695"
     CDS             1031..1915
                     /locus_tag="PKH81_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96976.1"
                     /translation="MAIILSHDNLKLTCELAGERYRSSRFDWNGLVSSARWKGIELLG
                     EEKPLFQRDPSRFGRALHNEFGIKTCIGYDEVAVGQWFPKIGTGWLKKDTKPYFFYNE
                     YQMEALSFTADHDGANALIFSCLSGERNGYAYEYQKRISLADKGFAITYTLTNTGERP
                     LQTDEYVHNFLCFAGKRMDRGYKLSFPWVINESLLGEKVNPDKALALSGNTVECIRKT
                     SKQFYLGGLSNGHASPSALWTLSHDGLGISLSEAGSFVPSAVHLWGWKRVISPEVFFS
                     FSIARGETVSWERRYSVT"
     gene            complement(1900..3015)
                     /gene="alr"
                     /locus_tag="PKH81_02700"
     CDS             complement(1900..3015)
                     /gene="alr"
                     /locus_tag="PKH81_02700"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNQ96977.1"
                     /translation="MHPTRAIIHLGNLAHNIEEIRRLVGAHRRICVPVKADAYGHGAI
                     RIARELLAEGVSHLAVATVSEAAALRAADITAPILLLGLVIPEEYPLLVQTGCEAFVA
                     DKDQIAGLALQAKQMNTSLAVHLKIDTGMGRIGCVPQEAPSLARYILDSGLSLAGVAT
                     HLPVADSAKPQDIDFTHAQIKRFTDAVTSIRDAGIDPGIVHAANSGGILQYPESWLDM
                     VRPGIIVYGYPSDPSSLTGHTIELKPVMKLETRVVHMKKVQKGTSISYGRTWTAERDT
                     VIATLPVGYGDGLARSLSNRLTVRIAETDYPIVGRICMDQCMIDVGPEPAVRRWDTAV
                     IFGPGHGADALARMIDTIPYEITCSIAPRVPRVYVTE"
BASE COUNT          793 a          759 c          896 g          848 t
ORIGIN      
        1 ttaaacacaa ttgccggttt gcgagctaca ccttgcttac caagggaagc tatccggccg
       61 agctggatat ccgccttcct ttttccatgg tattggaaaa tcgcaaaacc gatcggctcg
      121 aggttatgcc agcgtattac tggatgtaca acatgtatgc cctggaacgt aatagctgga
      181 aataccgcac ccgggataaa cggaagaata ttgtacagcg gatagaaacc gatcaccttg
      241 cccccgacac tgcccgagaa atgatcgaag gaatgcgctt gttggagata tggacgggta
      301 aggcttggta ttcctccgag tccgcccagg ggtctatgcc ttctgaagag gtcttggcgg
      361 aaaaggggcg agacttgatt acccgtgaac cggaaatcgt aaagagccta tttgtttctg
      421 gagaggcaat ggagagaggg aatcgcgagg ttcggattct caaggtagtg caggcctgga
      481 atgcctatcg acagatgctt cttttttacg ggatcaaaac gattgcctca tatctggatg
      541 agtacaacat ccattacgaa tattttagcg cgcaggtgcc tcagtctatc agccttgaat
      601 gggttaatct gggtgggcag cttgttcccg agcccaaggt agacggcctt cgttctgcgg
      661 taagaaccgg attgttttcg acctggaagg aaattcatga gcaatatgag tcctggtgga
      721 ctttgtatcc gcgcgataag gcagagaacg catatgcgat tctccggtta ctctgtaagt
      781 gcgacagtat ttccccggaa cgctggaatg ccctgcttga agaagctaaa ggaatacgaa
      841 cgtatatcga agagcaggta tacatcacca agaaaaaaga ttttgagaac tcgtaccgta
      901 tgattaccta tcgaaatacc gcagaacgcg atgcggtatt gggtactgtc gatgataacg
      961 gttttattag aacggcaaaa aaagaatcgg cagagttctt tgcgcttttg gaaagggttc
     1021 gggggtagtc gtggccatta ttctttcaca cgacaatctg aaattaacct gcgaacttgc
     1081 cggggagcgg tatcgctcaa gccgctttga ctggaatggt cttgtctcat ccgcgcgatg
     1141 gaagggtatt gaacttttgg gggaagagaa acccctgttt caacgagacc cgagtcgctt
     1201 tgggcgagct cttcataacg agttcgggat aaagacgtgc atcggctatg acgaggttgc
     1261 agttggacaa tggtttccaa agataggcac tgggtggctc aagaaggaca ccaagccgta
     1321 ttttttttat aacgaatatc aaatggaagc gctttctttt acggcagatc acgatggggc
     1381 aaacgcgctt atcttttctt gcctctccgg ggagcggaat ggctatgcct atgaatacca
     1441 aaagcgcata tcccttgccg acaagggatt cgccattacg tataccctta caaatacggg
     1501 cgagcggccc ctccaaacag acgaatatgt tcacaacttt ctctgttttg ccgggaaacg
     1561 gatggacagg ggatataagc tttcttttcc ttgggtaatc aatgaatccc ttcttggcga
     1621 aaaggtgaat ccagacaagg ccttggcgct ctctgggaac acggttgagt gtatacgcaa
     1681 aacttccaag cagttttatc ttggaggtct atccaatgga cacgcttctc catctgccct
     1741 ttggacgctt tctcatgacg ggttggggat atccctcagc gaagcgggat catttgttcc
     1801 atcagccgtt catctgtggg gatggaagcg agtaatcagt cccgaggtct tttttagttt
     1861 ttccattgca cgaggcgaga cggtcagctg ggaacggcgc tactcggtta catagactcg
     1921 gggtacgcgg ggtgcgatag agcaggtaat ctcgtagggg atggtatcga tcatccgggc
     1981 aagcgcgtct gctccgtgcc cggggccaaa gataaccgcg gtatcccatc tgcgtactgc
     2041 aggttctggc cctacatcga tcatgcattg atccatgcaa attcttccga ctattggata
     2101 atcagtctct gcgatgcgaa cggtaagccg attggaaaga gatctggcca aaccgtctcc
     2161 atacccaacg ggtaatgtcg cgattaccgt atcgcgctcg gcagtccacg ttcttccgta
     2221 cgagatgctg gttccttttt gtaccttttt catgtggact acccgggttt caagtttcat
     2281 taccggcttt aactcgattg tgtgtccggt aagagaggac ggatcgcttg ggtatccata
     2341 tacgataatt ccggggcgca ccatgtcgag ccaggattcg ggatattgga gaatccctcc
     2401 tgaattggcg gcatgaacga tgccgggatc aatgcccgca tcgcggatgg aagtaacggc
     2461 gtccgtaaat cgcttgatct gggcatgggt aaagtcgata tcttgaggtt ttgctgaatc
     2521 ggcgacgggt aagtgggttg caactccggc aagagaaagg cctgaatcca ggatgtaacg
     2581 ggcaagggat ggcgcttctt ggggcacgca tccgattctg cccatgccgg tgtctatctt
     2641 gaggtgcacg gccagggagg tattcatctg tttggcctgt aaggcaaggc cagcgatttg
     2701 atctttatcg gctacaaagg cttcgcagcc ggtttgaaca agcaggggat attcttcggg
     2761 gattaccaga ccaaggagga ggattggagc agttatgtct gctgcccgga gggctgctgc
     2821 ttcagagacc gtggcaaccg caaggtgact tactccctca gccaacagct cccgcgcaat
     2881 gcggatagct ccatgaccat aggcatctgc cttgaccggc acgcagatgc gtcggtgtgc
     2941 tccgacaagg cggcgtattt cctcaatatt atgagcaagg ttccctagat gaataatcgc
     3001 tcgtgttggg tgcatgctgt atcttacaac cccgcacacg ggctgacaag atgtcgaaaa
     3061 cactggaatt attggagttt tggtgatatc ctgtagccaa caaaaagttt attttgttta
     3121 tttgcgtgtt agcactcaat caatctttct catgaggtaa ctattgtgca caatgccata
     3181 gttcgcagtc ttatctgcgt aagtattctg ggtatcttct tttcatgtgc ttcttctccg
     3241 tcttcttcta ccgaagaatc tggaacggtt gagcgctttg gcgccggcgg aattga
//
LOCUS       DAOQXQ010000116         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_30809_length_3279_cov_2.670596, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000116 DAOQXQ010000000
VERSION     DAOQXQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30809_length_3279_cov_2.670596"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="PKH81_02705"
     CDS             complement(<1..1897)
                     /locus_tag="PKH81_02705"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HNQ96978.1"
                     /translation="MKKNTTVYYLHAVFLALSCLIGIPFFLMYQDVVARSVQSFLSLR
                     TNPDLLGGKIATVLYDPVGDDSGFGSIVYPSFSDGQGSLDIVRYTVYQPVRGSKGPGL
                     RDYWQLGFTFRAASTQRNIRVYLGTQDGHGGSVHTKTEFAEGIEFSSRFPWQYLISII
                     GEKGTIESFDGTYKQDVPVYSLLAGTELVLRIPLADEELSFLYDFPDMYQYVLVGLND
                     IQGNGGFSGNGVSDFAPNLYDIVVPEGFLQSELLSGWSEEDFTLATLEPVPIRLSKSV
                     EEDSQGVESTDETIAAYQTLKKNAATEREEKQRQSRQRYASPSLEPFEAAVAAFNAGM
                     YDDAEKLFDTLLSTDRRSSPAMAYKGSLVALRADKAPPLEAVGIVKEAYEWLDRSVKG
                     AQGDLELYTALMNRASVSLAVPDAIFGYAQQGGDDFIRASECVVRLLKSKDASVGVTR
                     EDYIGAVCNAALCYEISGDTVKAETWFRETKRMLQDTDETLCAFERMETFRYFELGEE
                     KVEEKKSRRKEDKDTTKAEDKRNAQATELASVLSLLDSNPGDLQLVEKALRLQIMELD
                     AITDGLALANAHKGILADSLYGQLFIAVAECKMATKVKKVVEKVDWITQGMRRFETIQ
                     RRWPNNET"
     gene            complement(1894..3057)
                     /locus_tag="PKH81_02710"
     CDS             complement(1894..3057)
                     /locus_tag="PKH81_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ96979.1"
                     /translation="MTLLISGLYIACMVLMVKLFVIAGSPSSIGSWLYSLFLVVIFSR
                     MILTRRFSKYRRISTSVFTLLFALSYIWTMVDNYGSILLGEAAVLSGTIPFNPFLLPV
                     LPLYLIFSRSLPFSAVITGCVLSVTGLALIWSMAAITIGKGWCSWVCPFGGYEDFFSR
                     IRKKPLIHLDGCANRLQIFNTWFAAIVVLLSVLCVIPVYLYYLNPFSLITGFYNTGGL
                     IGLFITAFLIILFICLVIILPYLTKKRFQCISYCPLGALQNLVSRAACVRTVIDMDAC
                     DQCMKCVNECRFSAIDKSSIQSRKPMVLSSCAHCGECIEVCPNKAIRFEYSFVRSGTT
                     KPSAQSRFARVVQCLIDPERVYVFSAFVFFVFFSSSFATDALNRIIACIGGLV"
     gene            complement(3061..>3279)
                     /locus_tag="PKH81_02715"
     CDS             complement(3061..>3279)
                     /locus_tag="PKH81_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96980.1"
                     /translation="YKVMKTDIDWLSSRRSTLEAHDQSLLDLYKGLYLIKARKLSEAI
                     ASFESCIAVAPGSSEAEEAQKQLNKYGE"
BASE COUNT          988 a          811 c          712 g          768 t
ORIGIN      
        1 ccgtttcgtt attcggccat cggcgttgaa tagtctcgaa tcgacgcata ccctgagtga
       61 tccaatctac cttttctacg acttttttca ccttagttgc catcttgcat tcggcaactg
      121 cgataaaaag ctggccgtac agggaatcgg ccaggattcc cttatgtgca ttagccaggg
      181 caagaccgtc tgtaattgca tcaagctcca ttatctgaag gcgcaaggcc ttttccacca
      241 attggagatc accgggattc gaatcaagga gggagagaac ggatgcaagc tcagtagctt
      301 gagcattccg cttgtcttcg gctttagtag tgtccttgtc ttcttttcgt cgggattttt
      361 tttcttcgac cttctcctcg ccgagctcaa aataccgaaa ggtttccatg cgctcaaacg
      421 cacacagggt ttcatcggta tcctgcagca tacgttttgt ctcgcgaaac caggtttctg
      481 cctttacagt atctccagat atctcgtaac agagggctgc attacaaact gcaccaatat
      541 agtcttcgcg ggttactcct acagaagcat cctttgactt gagtagccgc actacgcact
      601 ccgaagctct tataaagtca tcaccgcctt gctgtgcata tccaaaaatc gcatcaggaa
      661 cggcaagcga aacgctcgct cggttcatta gcgcagtata cagctccaag tctccttgcg
      721 cgccttttac tgagcgatcg agccactcat atgcttcttt aacgatgcca accgcttcca
      781 atggcggagc tttatcggct cgcaaggcga caagagatcc tttatatgcc attgccggag
      841 aagatcgtcg gtcagttgat aatagtgtat caaaaagctt ttccgcatcg tcatacattc
      901 ccgcattaaa cgctgcaacc gctgcctcaa agggttcaag agatggggag gcatatcttt
      961 gcctggactg ccgctgtttt tcttcacgct cagtggcagc gttcttttta agtgtttgat
     1021 atgccgctat tgtttcatcg gtagactcca cgccctgact gtcttcttcg acactcttag
     1081 aaagacgaat cggaaccggt tctaaagtcg caagtgtaaa atcttcctcc gaccatccgg
     1141 atagcagttc cgattgaagg aagccttctg gcaccactat atcgtacaga ttcggggcaa
     1201 aatccgatac tccgttaccg gaaaatccgc cattaccctg aatgtcattc aaaccaacaa
     1261 gtacatactg atacatatca ggaaaatcat ataaaaagga tagctcctca tcggcaagag
     1321 ggatacgcaa caccagttcc gttcctgcca aaagcgaata taccgggaca tcctgtttat
     1381 aggtgccatc gaaagattca atcgttcctt tttccccaat gatggagata agatactgcc
     1441 aggggaaacg agaggaaaac tcaataccct cagcaaattc cgtcttggtg tgtactgaac
     1501 cgccatgccc atcttgagtc cccaaataca cacggatatt tcgctgtgta gacgcggcac
     1561 ggaaagtaaa tccgagctgc cagtaatccc ggagcccggg tcccttactg cctcgtacgg
     1621 gctgatacac tgtataacgg acgatatcaa gcgatccctg cccatcagaa aaagaaggat
     1681 acacaataga tccaaaacca gagtcgtctc caacaggatc gtacaagaca gtcgcaatct
     1741 taccaccaag aagatcagga ttggtgcgca atgataaaaa agattgcaca gaccgggcca
     1801 caacatcctg atacattaag aaaaatggta tgccgatcaa acaggacagt gccagaaaca
     1861 cggcatgaag gtaatacaca gtagtgtttt ttttcatacc aggcctccaa tacatgcaat
     1921 gatgcggttg agtgcgtctg tggcaaagga acttgaaaaa aacacaaaga acacaaaggc
     1981 ggaaaaaaca tagacgcgtt ccggatcaat aagacactga acaacccggg cgaaacgact
     2041 ttgcgcagaa ggctttgtgg taccagagcg aacaaacgaa tactcaaaac ggatagcctt
     2101 attagggcat acttcaatac attccccgca atgagcacat gatgagagca ccattggttt
     2161 acgcgattgt attgacgatt tgtcaattgc agaaaatcga cattcattca cgcacttcat
     2221 gcactggtcg catgcatcca tatcgataac cgtccggaca cacgcagcgc gcgatactaa
     2281 attctgtaaa gccccaagcg gacaatacga tatacactga aatctttttt ttgtgaggta
     2341 cggaagaatt atcacaaggc agataaaaag aatgataaga aacgcagtaa taaagagacc
     2401 gatcaacccg ccggtgttat aaaacccggt aataaggcta aatggattaa gatagtacag
     2461 gtaaactgga atgacacaga gcactgacaa caacacaacg attgccgcaa accatgtatt
     2521 aaaaatttgt aaacggtttg cgcacccatc aaggtgtatc agcggttttt tacgtatacg
     2581 cgaaaagaaa tcctcgtacc ccccaaaggg acacacccag ctacaccacc ctttaccgat
     2641 agtgatagca gccattgacc aaataagggc aagaccagtt accgacaaaa cacagccggt
     2701 gatgacagca gaaaatggga gagaacggga aaaaatgaga tataacggca gcacgggaag
     2761 aagaaacgga ttaaaaggaa ttgttcccga caataccgct gcctctccca gcagtatgct
     2821 tccatagtta tccaccatcg tccagatgta cgagagtgca aagagtagtg taaaaacgct
     2881 tgtactgatt cttctatatt tagaaaagcg gcgggtaaga atcatccggg aaaaaataac
     2941 aaccaagaat agcgagtaaa gccaggagcc tatagaggaa ggagaacccg ctatcacaaa
     3001 gagtttgacc atcaggacca tacacgcgat atacaaacca gaaatgagta gtgtcatccc
     3061 ttactccccg tacttattga gttgcttctg cgcttcttct gcctcgctcg atccgggagc
     3121 aacagcaata cacgattcaa aagaggcgat cgcctcgctg agcttacgcg ctttaataag
     3181 atacaaaccc ttgtataaat ccaggagaga ctgatcatga gcttcaagcg tacttcggcg
     3241 agaggaaagc cagtctatgt cggttttcat taccttgta
//
LOCUS       DAOQXQ010000117         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_30962_length_3267_cov_3.078456, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000117 DAOQXQ010000000
VERSION     DAOQXQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30962_length_3267_cov_3.078456"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PKH81_02720"
     CDS             complement(<1..1307)
                     /locus_tag="PKH81_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ96981.1"
                     /translation="MSDNHSSDELVRRYRAVHYLFSSMNSVKDPNELFQLVLSHCVAQ
                     TSSDYGAILLWDGDETLETRLVHPAHPKSEATEKPRIRVGEGLAGRTFLDGVPRSLDN
                     TAHDPEYIPIAGSFSSILVLPFGAPGKVRGVIRLESSKTGNYSEHDSQILLPVTALAG
                     QLLSQIEIKTSLEWTIRQKDILIDISRNVAKHFDLNEVFGTVMHRLAESFNIMRGMLV
                     LLDSDNPNALSVRAAYNLTDEEMSRGIYTVGEGVIGKVVESGEAISIRDIYTDKNFLN
                     RMKVKRRKDVPISFIAVPFRVDGQVAGVIAVEKAFESDDVLKDEKDLMILVSTLLITK
                     IKVFQSVTGEHERLIEENSTLKQELKKRYEDSSIICKNRKMLELLDLVHLVADSNSSV
                     MILGESGTGKELIAREVHRESNRSEGPFISINCAAIPDSLLES"
     gene            1609..>3267
                     /locus_tag="PKH81_02725"
     CDS             1609..>3267
                     /locus_tag="PKH81_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNQ96982.1"
                     /translation="MFNEMYGSASFGDAAMRERLPKAVYKQLKEVQAGEAELTMEIAE
                     IVAASMKEWAIAQGATHYTHWFQPMTGLTAEKHDTFISPTNDGRANLEFSGKELIRGE
                     PDASSFPSGGLRATFEARGYTVWDVTSPAFINVEDNGCKTLVIPTAFISYNGEALDKK
                     TPLLRSMQAINKQAMRVLKGLGNETAKRVITTVGPEQEYFLVDADYFHAREDLVLTGR
                     TLFGAMPPKGQEMEDHYFGTISAKASAFMADLNDELWKLGISAKTQHNEVAPNQFELA
                     PIFSVSNVACDHNQLIMQTMKKVAIRHGLVCLLHEKPFRGVNGSGKHNNWSIGTDDGM
                     NLLDPGSTPHENLQFMVFVSAVLAAVDRYAPLLRLSAANTGNDHRLGANEAPPAIISV
                     FLGEQLEEIFEAVSNGKGSSAKKGIDLELGVTSLPKLPKDLTDRNRTSPFAFTGNKFE
                     FRMVPSSDSIAGPNFVLNTVVAEVLSEFADVLEKGGDVKTLSKSLISDVWKKHKRIVF
                     NGNGYTEDWIAEAKRRGLPNLTSSVEAWPYFGSDESVALFGKHNV"
BASE COUNT          817 a          771 c          779 g          900 t
ORIGIN      
        1 tctgattcca ataagctgtc gggaatagct gcacagttaa tggagatgaa cggcccctcg
       61 cttctgtttg attcgcggtg tacctctctg gcgatcaatt cctttcctgt gccgctttcg
      121 cccaggatca ttacagagga atttgaatca gctaccaaat gaaccagatc aagaagttca
      181 agcattttgc ggtttttgca aataatggat gagtcttcgt atcttttttt cagttcttgc
      241 ttaagggttg agttttcttc aatcagtcgc tcatgttcac ccgtgacact ttgaaagacc
      301 tttatctttg ttatcagaag ggttgaaaca aggatcatca agtctttttc gtccttgagt
      361 acgtcatcag attcaaaggc tttttctact gcgataacgc ctgctacctg tccatcaacg
      421 cgaaagggaa ctgctataaa cgagatagga acatcctttc ggcgcttaac cttcatacga
      481 ttgagaaagt ttttgtcagt atagatatcg cggatagata tggcctctcc actttctact
      541 accttaccaa taactccttc gccaacggtg taaatcccac gagacatttc ttcgtctgtt
      601 agattatatg cggcacgcac cgataatgca tttgggttgt cgctatcaag aagtacgagc
      661 atgcctcgca tgatgttaaa actttccgcc agacggtgca tgacggtgcc aaagacttcg
      721 ttaagatcaa agtgttttgc aacgtttcgc gatatgtcta tcaggatatc tttttggcgg
      781 attgtccatt ccagcgaggt tttaatctcg atttggctca ggagttgacc ggcaagggcc
      841 gtaaccggaa ggagtatttg cgaatcatgt tcgctgtagt ttcccgtttt gctcgactca
      901 agacgtataa cgccccgcac ctttcccgga gcgccaaagg gcagcaccaa tatagaagaa
      961 aaagaaccgg caatgggaat atattccggg tcgtgtgccg tattgtccag actgcgcggt
     1021 acgccgtcaa gaaaggttct tccggccaaa ccttcgccta cgcgaattct tggtttttcc
     1081 gttgcctcgc tcttggggtg agctggatga acgagccgtg tttccagggt ttcatctcca
     1141 tcccacagga gtattgctcc gtagtctgat gaggtttgag cgacacaatg agagagtacc
     1201 agttgaaaca gttcattggg gtcttttacc gagttcatgg aggaaaaaag ataatgtact
     1261 gcacggtagc gtctaacaag ttcgtctgaa gaatggttgt cgctcatatg atctccttgt
     1321 ccgattttga ttaaaaatcg ggcgtatgct atttattaag caatttctat gccaaaaatt
     1381 aaagtctttg ctatgcaagg atttaattat aatcactcag tacgataatg cctagatatt
     1441 gggcaccatc cagccagctg cttaatatgt aggcattgca caatacctat gcgtttatac
     1501 gcttttgcgt atctccttag ctgtaaagaa attagaaaag gctgagtact ctggcacgct
     1561 ttttgcagaa gtatatacaa gactagatga ccctggaggt tcacggtgat gttcaacgaa
     1621 atgtacggat cggcgtcctt tggcgacgcg gcgatgaggg aacgtcttcc caaggccgta
     1681 tacaaacaat taaaggaagt gcaggcggga gaagccgagc ttaccatgga gatcgcggaa
     1741 attgtcgccg cttccatgaa ggagtgggct atcgcgcagg gcgcaaccca ctacacccat
     1801 tggtttcagc ccatgactgg cttgactgcg gaaaaacacg acacctttat ctccccgacc
     1861 aatgacggcc gggctaacct ggagttttcc ggaaaagaac ttattcgggg tgaacccgat
     1921 gcctcgagct ttccttcagg cggtttgcgc gcgacatttg aagcacgcgg atatacggtc
     1981 tgggatgtaa ccagtcccgc ttttatcaac gttgaggata atggttgcaa gaccctcgta
     2041 atccccacgg cctttatctc ctataatgga gaagctcttg ataagaagac tccgctcttg
     2101 cgctccatgc aggcgataaa caaacaggct atgcgtgttc ttaaaggctt gggtaacgaa
     2161 actgccaagc gggttattac taccgtcggt cctgaacagg aatacttctt ggtcgacgca
     2221 gattatttcc atgcccgcga agatcttgtt cttactggcc gaaccctctt tggtgcgatg
     2281 cctcccaagg gtcaggaaat ggaagatcat tactttggta cgatctccgc caaggcctct
     2341 gcgtttatgg ccgatttgaa tgatgagctg tggaaactcg gcatttctgc caagactcag
     2401 cataacgagg ttgctccaaa ccaatttgaa cttgcaccga tcttttcagt ctccaacgtt
     2461 gcctgcgatc ataatcaact catcatgcaa acgatgaaga aggttgctat ccgccatggc
     2521 ttggtgtgtc tgctccacga aaaaccattc cgtggtgtga acggttcagg caagcataac
     2581 aactggtcta ttggaaccga tgacggcatg aatcttcttg atccaggctc gactcctcac
     2641 gagaatcttc agtttatggt ttttgtcagc gctgttcttg ccgccgttga ccgctatgcg
     2701 cccttgttac ggctctctgc cgcgaatact ggtaatgatc atcgtcttgg tgccaacgag
     2761 gctcctcctg cgatcatctc ggtctttctt ggcgaacagc ttgaagaaat atttgaggcg
     2821 gtatcaaacg gcaagggctc ttcggcaaag aaaggaattg atcttgagct tggcgttacc
     2881 tcattgccca agctgccaaa agatttaacc gaccgcaacc gaaccagccc cttcgccttt
     2941 accggaaaca aatttgaatt ccgtatggtt ccctcatctg attcaattgc aggacccaac
     3001 tttgtactga atacggttgt tgccgaggtg ctttccgagt ttgcagatgt tctggaaaaa
     3061 ggcggggatg ttaagacact ctccaaaagc ttgatcagcg atgtgtggaa aaaacacaaa
     3121 cgaattgttt ttaatggaaa cggatacact gaggattgga ttgccgaggc aaagcgccgt
     3181 ggcttaccaa acctgacttc ctccgtggaa gcctggcctt attttggcag cgacgagtca
     3241 gttgcactgt ttggtaagca taatgtg
//
LOCUS       DAOQXQ010000118         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_31506_length_3227_cov_3.492749, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000118 DAOQXQ010000000
VERSION     DAOQXQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31506_length_3227_cov_3.492749"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="PKH81_02730"
     CDS             <1..2430
                     /locus_tag="PKH81_02730"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96983.1"
                     /translation="YMNYGYFDDEHREYVITNPKTPVKWINYVGTLAFGGFIDHTGGS
                     QLCKGDPALGRITKYIPQLPAGEFKGETAYVRVRKPGLPAKDATVISPYWVPTLHAYD
                     RFECRVGMYYTRWITEAAGLRFDILAFIPRGSKELVRQYTITNTSGEPLQVDLVPVVE
                     FSHFDALKQLTNADWVPQTMQSHAIDTMDGNKAIAAYAFMKRDYAVNLFYANRSPSSW
                     ETDRKRFLGDNEYGTWRAPLSLNAESLSSQNSVRGDTIAALMYNLGTLAPNASETLIT
                     TLHQTESLQEAPRAATKWKTTAQIEKAFTDLGSFWDEYLSVIKVKTPDAAFNSMLNLH
                     NPRQCYTTKIWSRYLSLYQLGYGSDRGIGYRDSSQDTMGVMAQIPEEARELMEKLLSV
                     QSRDGSAYHQFNPLTMIAGRGDSLEYEDRPHYYSDDQLWIVLAVSSYLKETGNYDFLN
                     QEIPFYEKDKDEKPIEKGTVLEHLKRAVTFTHGNVGAHGLPLLGFADWNDTVNLPTGA
                     ESLFTANLYGWALRELSELAAYMGDEEQKKTWDDWYDTMKHTVLNTAWDGQWFVRYFD
                     DKGNPVGSAKNEFGKLWLNGQSWALLSGFAKDSDKGRTCMDSVKKHLATDKGIKLSWP
                     GYNGYDHNKGGVTTYPPGAKENGGIFLHPNPWAIIAETILGNGDQAFDYYTRINPAAK
                     NDSIDEYECEPYCYAQNILSNEHPNFGLGRNSWLSGTSSWVYQASTKYILGIRCEHEG
                     LRIDPCVPASWKEFSIERKCRGAVYQITVKNPSGTSKGVSELVVDGKTIPGSLVPYFK
                     DGTHTVEARMR"
     gene            complement(2489..>3227)
                     /locus_tag="PKH81_02735"
     CDS             complement(2489..>3227)
                     /locus_tag="PKH81_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96984.1"
                     /translation="TRLGSVLAYIGIISRNLVPLAVLVAGIGLAVWNRIASAKKIRKE
                     DEAQPVSARVLDARYEAGSTALFLFIFLTTFSIIAVSAPYQTVRYVAAFFPVYVLAFT
                     GLMRMVLSSRQARVLVAAAALLIAIHGLQKSNLLDFHEDYTTDTPAYYMTDEHPLIMM
                     GTIGGTWKTPLVYVNIASSKDLYVTGADYGANLSRRLDSIADEIVNAKGGNTVYALVC
                     DYFENKPGWEEIGYYGFYWVFKKSYSQ"
BASE COUNT          874 a          863 c          730 g          760 t
ORIGIN      
        1 tatatgaatt acggctattt tgacgacgaa cacagggagt acgtaattac caatcccaaa
       61 acaccggtca aatggatcaa ttacgttgga acccttgcgt tcggcggctt tattgaccat
      121 accggtggct cacagctgtg taagggagat cccgcgcttg gacgcattac caaatatatt
      181 ccccaacttc ccgcagggga gtttaaggga gaaaccgcct atgttcgcgt cagaaaaccc
      241 ggccttccgg ccaaggacgc caccgttata tctccttact gggttcctac cctccacgcc
      301 tacgaccggt ttgaatgtcg tgtcggcatg tactacaccc gctggattac cgaagcggca
      361 ggactccgct ttgacatcct tgcctttatt ccccgtggca gcaaggagct tgttcgccaa
      421 tatactatta ccaatacaag cggcgaaccc ttgcaggttg accttgtacc ggttgtagaa
      481 ttctctcact ttgatgccct taagcagctt accaatgccg actgggttcc ccaaaccatg
      541 caatcccacg cgatagacac catggatggg aataaggcta tcgcggctta cgcctttatg
      601 aagcgggact acgcggtaaa ccttttttac gctaaccgct ctccctcgtc ttgggaaaca
      661 gaccgcaaga gattccttgg cgacaatgag tatggaacct ggcgagcccc cctgtcccta
      721 aacgctgaat ccctttcttc tcaaaacagc gtcaggggag acactatcgc agcccttatg
      781 tacaatctgg gaacccttgc tcccaacgca agcgaaaccc tgattactac cctccatcaa
      841 accgaaagtt tgcaggaagc gccgcgcgcc gctaccaaat ggaaaaccac ggcccagata
      901 gaaaaggcct ttacagacct tggctctttt tgggatgagt atctttcggt tatcaaggtt
      961 aaaacacccg atgccgcatt taactcaatg cttaatcttc acaatccccg tcagtgttac
     1021 accaccaaga tttggtcccg atatctgtct ctctatcagc ttggatacgg ctcagaccga
     1081 ggcatcggct atcgtgattc ttcacaggac accatgggcg ttatggccca aatacccgaa
     1141 gaagcacgcg agcttatgga aaaacttctt tccgtgcaaa gccgcgatgg ctcagcctac
     1201 caccagttta acccgcttac catgatcgca ggccgaggcg actcccttga atatgaagat
     1261 cgcccacact attactccga cgatcagttg tggattgtcc ttgccgtctc atcctatctt
     1321 aaggaaactg gtaactatga cttccttaat caggaaatac cattttacga aaaggataaa
     1381 gatgaaaagc ccatagaaaa aggaactgtc cttgagcatc ttaaacgggc cgtaaccttt
     1441 actcatggaa acgtgggagc tcatggcctt ccccttcttg gttttgccga ttggaacgat
     1501 accgttaatc ttcccaccgg ggcggaaagc ctttttaccg ccaatctcta tggctgggcc
     1561 ctgcgagaac tttctgagtt ggccgcctat atgggcgacg aagagcaaaa gaaaacctgg
     1621 gacgactggt atgatactat gaagcacact gtgcttaaca cagcctggga cggccagtgg
     1681 tttgtccgct attttgacga caagggaaac ccagtcggtt ctgcaaagaa tgagtttggc
     1741 aaactgtggc ttaatggtca gtcatgggcg ctactgtccg gattcgcaaa ggacagcgac
     1801 aagggaagaa cctgcatgga ttcggtcaag aaacatcttg ctaccgacaa gggcatcaag
     1861 ctttcctggc cggggtacaa cggatacgat cacaataagg gtggcgttac cacctatccg
     1921 ccgggagcaa aggaaaacgg tggtattttt ctccatccca atccatgggc aatcatcgct
     1981 gaaaccattt tgggcaatgg cgaccaagcc tttgattact acacgcgcat taaccctgcc
     2041 gcaaaaaacg attccatcga tgaatatgag tgcgagcctt actgctatgc gcaaaatatt
     2101 ctatccaacg aacaccccaa ctttggcctt ggacgaaatt cctggctttc gggaacctca
     2161 agttgggtat accaagcttc caccaagtac attcttggaa tacgctgcga acacgaaggc
     2221 ttgcgtatcg acccctgcgt accggcatcg tggaaggagt tttccatcga acgcaagtgc
     2281 cgcggcgcgg tgtatcagat tacggtaaaa aatccctccg gtacatccaa gggtgtgtca
     2341 gagcttgttg tggacggaaa aacaattcca ggttcgcttg ttccttattt taaggacgga
     2401 acacatactg tagaagcccg aatgcgctaa tatgcaaaag gccgaggaag cgattcctcg
     2461 gcctttatct tatccaaaca tacgcctgct attgactata gctctttttg aaaacccagt
     2521 aaaaaccata atatccaatc tcttcccacc ccggcttatt ttcaaagtaa tcacagacca
     2581 gtgcatacac ggtattcccg ccctttgcat tgactatctc gtcggcaata ctgtcaagtc
     2641 gtctggacag atttgctcca tagtctgctc cggtaacata caggtccttg gaagaagcga
     2701 tgtttacata caccaagggt gtcttccatg ttcctccaat tgttcccatc ataattaggg
     2761 gatgctcatc agtcatgtaa taggcggggg tatcggtagt atagtcttca tgaaaatcca
     2821 gaaggtttga cttttgcaat ccatggatag caatcaaaag agccgcagca gcgacaagaa
     2881 cccgtgcctg cctggagcta agcaccatgc gcatcaagcc ggtaaaggct aacacataga
     2941 cggggaaaaa agccgcaaca taccggacgg tctgatacgg agccgatacg gcaataattg
     3001 aaaaggtagt taaaaaaata aacaaaaaca acgcggtact tccggcttca taacgagcat
     3061 ccagcaccct tgcggaaaca ggctgcgcct catcttcctt acgtattttt ttggccgatg
     3121 caattcggtt ccacacagca agtccaattc ccgccaccag aacagcaaga ggaacaagat
     3181 tacgggagat aattcctatg tacgccaaca ccgaaccaag acgggtc
//
LOCUS       DAOQXQ010000119         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_31873_length_3203_cov_2.925349, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000119 DAOQXQ010000000
VERSION     DAOQXQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31873_length_3203_cov_2.925349"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKH81_02740"
     CDS             <1..402
                     /locus_tag="PKH81_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96985.1"
                     /translation="LSYHPPELDEAVRTNSRDPRLILWAPDPVALFSPLLQGIPLLNL
                     SAPARDLIVFAQRDATTSDRYNLSARFETTDAKNARAFSALFRLAIKSFFRTTEGITE
                     GTITSEGTFIRMEGYSLSDDSLADLIRFFYF"
     gene            437..736
                     /locus_tag="PKH81_02745"
     CDS             437..736
                     /locus_tag="PKH81_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ96986.1"
                     /translation="MPTYDYACDWCGHRFEAVQKMADAPLETCPECGKKIRRVLSGGI
                     GISFKGSGFYVNDASSGSSAATSKPAKTESVKSTPSTACASCPSKASGTTKGKAE"
     gene            740..1054
                     /locus_tag="PKH81_02750"
     CDS             740..1054
                     /locus_tag="PKH81_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ96987.1"
                     /translation="MAKKPRFFCEHCGAEVRQRDKVCSRCGRFFASVKCPACGKSGTS
                     RDFSRGCPRCGYAFNPDGSAQAGGYRRGKNTSADPLPWWMYVATIALLVCVLAFILSL
                     AR"
     gene            complement(1071..1760)
                     /locus_tag="PKH81_02755"
     CDS             complement(1071..1760)
                     /locus_tag="PKH81_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96988.1"
                     /translation="MIALLIADSEDYITPLSTLLEGWAFDVIRYRSAIKALDNLEEIS
                     PDAVIISAKDFPRHWKALVQYIRSDSSRSETVIALVVNERFSSEEADKASFIGVQAII
                     REDIESDKDKLLEIFSRYRYVGKAPSRRVFEITREQTEFLFTNPKSGVIITGRLDSID
                     TQSLVFKCDCPSETSDLVIGDLIDRCSLKIQEKFFTPRCTVTKNGSLIELSFDEPSAD
                     LVATIQDLLEA"
     gene            complement(1784..2755)
                     /locus_tag="PKH81_02760"
     CDS             complement(1784..2755)
                     /locus_tag="PKH81_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96989.1"
                     /translation="MKQLFSVLTIALVCSLMTATAEEILPGAAFSIRYHDRSIYYPGN
                     SPSEPINIQLTITNKGPQTLRFKLADDRSFSLYFTARTTRGQALENAPAWMIKRAANR
                     EVYFREVSLEPGESFSIVENLKDYLVITAPGMYVLDCDFYPELKRLSGSSEPSIASNR
                     LTLEIKPSPSAAATSVMPIHPKTAEILKPQAMPPDEVISYILTSRQRSHWDQFFLYLD
                     LDEMIKRDPARKRRFAAESEEGRIAMIDNYKNELSQARIDKDISTIPYKFQIERTTYA
                     GNDGVVTAIEWFQYPTFAEKKRFTYYLVSRDGIWRVVNYTVDNLGTE"
     gene            2826..>3203
                     /locus_tag="PKH81_02765"
     CDS             2826..>3203
                     /locus_tag="PKH81_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNQ96990.1"
                     /translation="MDAQFLKKALHKWPALQAVMSRLAENRWPCHIEGVQGGLHAFFL
                     SECLSTLPSPPIIVVPTEKDIELLAADLSLAGLQSEVFPWWGTMAYRPVAPGAAVFGQ
                     RSACLASLLSPSSASRILVTTERA"
BASE COUNT          788 a          796 c          800 g          819 t
ORIGIN      
        1 ctctcttatc atccaccaga gcttgatgag gcggtacgta ccaactcccg cgatcctcgc
       61 ctgattcttt gggcccccga tccggtagcc cttttttcgc ctttgttgca aggtataccg
      121 cttttgaacc taagcgcacc tgcgcgggac ttaatcgtct ttgcccaacg tgacgctact
      181 acaagcgacc ggtataatct gagcgcgcga tttgaaacaa ccgatgcaaa aaacgcgcga
      241 gctttttctg ccttgttccg ccttgccatt aagtcctttt tccgaaccac cgaggggatt
      301 accgaaggca cgattaccag cgaaggcacc tttatacgca tggaagggta ttccctgagc
      361 gacgattctc ttgccgattt gattcgtttt ttttattttt aaaagtagta tagtacacga
      421 gagaggaacc acagcaatgc ctacctatga ttatgcctgc gattggtgcg ggcaccgatt
      481 tgaagcggtt caaaaaatgg ccgacgcccc gttagagact tgtcccgaat gtggaaaaaa
      541 aattcgtcgc gttctttcag gcggcatagg aatttccttt aagggctcag gcttttatgt
      601 taacgacgcc tcatctggct cttctgccgc cacaagcaaa cctgcaaaaa ccgaatctgt
      661 aaaaagcact cccagtacag cctgcgcctc atgtccatcc aaggcatctg gcactacaaa
      721 gggaaaggcg gaatagcgga tggcaaaaaa accacgcttt ttctgcgagc actgtggtgc
      781 cgaggtacgc cagagagaca aggtgtgttc tcgatgcggc cgtttttttg cctcggtaaa
      841 atgccccgca tgcggaaaaa gcgggacgag ccgcgatttt tctcgtggtt gtccacgctg
      901 tggatatgcc tttaatccgg acggctcggc tcaagcggga gggtatagac gcggaaaaaa
      961 caccagcgcc gatcccttgc cctggtggat gtatgttgca acaattgcct tgttagtgtg
     1021 tgtcttggcc tttatcctgt cgcttgcgcg ataaggtgcg gttaggctta tcaggcctct
     1081 agaagatctt gtatcgtagc gactaaatcc gcggaaggtt catcaaacga aagctctatc
     1141 agggagccgt ttttggtaac ggtgcagcga ggggtaaaga acttttcttg aatcttgagg
     1201 gaacaccgat caattaaatc accaatgacc agatcgcttg tttctgacgg acagtcgcac
     1261 ttaaagacaa gcgactgtgt atcgatcgaa tcaagccggc cggtaataat gacgccgctt
     1321 tttggattag taaacaagaa ctcggtttgc tcgcgggtaa tttcaaacac gcgacgggag
     1381 ggggcctttc ccacatagcg atagcgagaa aaaatttcca gtaacttgtc cttatcgctt
     1441 tctatatctt cccgaataat cgcctgaacc ccaataaacg atgccttgtc ggcttcttca
     1501 gacgaaaaac gctcatttac cactaaggcg ataaccgttt ctgatctgga ggaatcggat
     1561 cggatatact gcaccaaggc tttccaatgt cggggaaaat ccttggcgct aatgattacc
     1621 gcatcagggg atatttcttc caggttatca agagccttaa tcgcggagcg atagcggata
     1681 acgtcaaagg cccatccttc aagaagggta gaaaggggtg tgatatagtc ttctgagtca
     1741 gcaataagaa gcgcaatcat gcaggtttat ttcctttatt ggattactca gtgcccaagt
     1801 tatctacagt gtagttcact acccgccaaa taccgtctcg cgaaactaaa taatacgtaa
     1861 atcgtttttt ctcggcgaag gtaggatact ggaaccattc aatggcggtt acaacaccgt
     1921 cgttaccggc gtaggtggtc ctttcaatct gaaacttata tggaatggta gagatatcct
     1981 tatcaattcg cgcctgactt aattcgtttt tgtagttatc gatcatcgcg atgcgtcctt
     2041 cttcgctttc ggcggcaaag cggcgtttgc gtgcggggtc gcgcttgatc atttcgtcca
     2101 gatcaagata caaaaagaat tgatcccagt gtgacctttg ccgtgaagta aggatgtaac
     2161 ttattacctc gtcaggcggc atcgcctgtg gcttaagaat ttcggctgtt ttaggatgaa
     2221 tgggcatgac cgaggttgcc gcagcagatg gggatggttt aatctccagc gtcaagcggt
     2281 tggaggcaat cgaaggctcg gaggagccgg aaagacgctt gagctctgga taaaaatcgc
     2341 aatcaagcac atacatccct ggagcagtga taaccagata atccttaagg ttttctacaa
     2401 tagaaaaaga ttctcccggt tcaagagaaa cctcgcggaa gtacacctcg cggttagctg
     2461 cccgctttat catccaggca ggcgcgtttt ccagagcctg acccctggtc gtgcgggcgg
     2521 taaagtacag gctaaaggag cggtcgtcgg caagcttaaa gcgtaaggtt tgcggcccct
     2581 tattggtaat cgttaactgg atgttgatcg gttccgacgg ggaattgccg ggatagtaga
     2641 tagatcggtc atgataacgt atggaaaaag ccgcgcccgg aagaatttcc tccgctgtgg
     2701 cagtcattaa cgagcaaaca agagcgatgg tcagtacaga aaagagctgt ttcacctgaa
     2761 attctccctt tttcattata tcggccttta tccccttcgt cattagtggt aaaatccgct
     2821 ccgttatgga cgcacaattc cttaaaaaag ccttgcataa gtggccagcc cttcaggccg
     2881 taatgagtcg actcgcagaa aaccgctggc cctgccatat agagggagtc cagggaggac
     2941 tccacgcgtt ttttttatca gaatgcctta gtacccttcc gtctccgccc atcattgtag
     3001 tgccgacgga aaaggatata gagctattgg ctgcggactt aagtcttgcc ggccttcaaa
     3061 gtgaggtctt cccctggtgg ggaaccatgg cttatcgccc ggtagccccg ggggcagctg
     3121 tctttggcca gcgctccgcc tgccttgcct ccctcttatc cccatcgtct gcctcccgca
     3181 tcctggtaac caccgaacgt gcc
//
LOCUS       DAOQXQ010000120         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_31875_length_3203_cov_2.641677, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000120 DAOQXQ010000000
VERSION     DAOQXQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31875_length_3203_cov_2.641677"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..337)
                     /locus_tag="PKH81_02770"
     CDS             complement(152..337)
                     /locus_tag="PKH81_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96991.1"
                     /translation="MLGFGDMGVFLGYALTLVAMVLCVCYGIRNWNRPREDIASEIME
                     EATWEANDPELGEGGDK"
     gene            complement(348..959)
                     /locus_tag="PKH81_02775"
     CDS             complement(348..959)
                     /locus_tag="PKH81_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ96992.1"
                     /translation="MKDTFFNLKEDKRERVLHAALICFARTGFEATSVDDIVAEAGIS
                     KGGLYEYIESKDDLYEYLVRYSYNALYDFIVSRKEPAGFSPDPVMRTRLIAYIAVEFY
                     SLKPEIIAFMVNSSRVEQGAVRTRTNEIFDSYFHRLYEDASFTELRFDKDRIIPLLKW
                     LLIKTRNDFTEAYYAHQEGDAAREVYLDQWEFFFSVLTEGIYR"
     gene            complement(1044..1820)
                     /locus_tag="PKH81_02780"
     CDS             complement(1044..1820)
                     /locus_tag="PKH81_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ96993.1"
                     /translation="MKVGLFGFGRTGRLVAEEIIHDPDCNLAWVVRKSAQNNHEYASD
                     FLGYPHNKQGYFFCMDEVGDTDFFKNNPVDVIIDFSSTQGVYEYAHAADRGIHIVSAI
                     SHYEKKERDFLRQLGEKTAVLYSANITLGINFLLVASQILQQIVPNADIEVIEEHFRE
                     KSGKSGSALKIAEKLGLDPDKHINSIRVGGIVGKHEVVFGLQNQTIRITHDVISRGAF
                     GAGALFAARALQKETTGFFTMEELMRAAFIQKIAVNNILA"
     gene            1989..3161
                     /locus_tag="PKH81_02785"
     CDS             1989..3161
                     /locus_tag="PKH81_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNQ96994.1"
                     /translation="MKSRASIRKQALPTIAESNRTVARILDLLQQKDTFLLLGHEFPD
                     EDCVASLVSMALIIHKCGKKVSLYLQNEIPGQLSFLAGICAYNKIPLLQGDDKPAEHP
                     QVIGILDTPKPDMIALNSAISGYLASPDIPIFEFDHHLSADAQYSGRPGLCLVNRASS
                     TCELIGFFCAKLSRRPDILATLGIRNLYSRNLVLSLLTGMIADTKFGITLKTDRDRFF
                     YNYFTNRFASILRDSNYKNSGNYSSLTDIFKSIQLLTVNERDVYLRLLSHTKVLGKVA
                     YIALDYENSHNYLTRLEPGLFVKVIKSVTDELCDKSEGIGLTAYYDMSETSDLIQFRI
                     RTSKSITNIDLRTILTEFSITDGGGHPGAIGFRIPKNQVSDIHTYIIPLLERLNTL"
BASE COUNT          922 a          817 c          628 g          836 t
ORIGIN      
        1 gctccatagg aaagcgccat aaccatcgga tgtatcttac gtccggcaag aagataatca
       61 gttgagttct tggtgctttt aaaaccggaa aaacccaaga cgcctacaac aaccaaatac
      121 actacaacca ctattgccaa aaatatcagg ttcatttgtc gccaccttct ccaagctcag
      181 gatcattcgc ttcccaggtt gcctcttcca taatctcgga agcaatatcc tcccggggac
      241 gattccagtt ccgtatgcca taacacacac agagcaccat ggccaccagg gtcagggcat
      301 aacccaagaa cacccccata tctccaaaac caagcatatg tatctcctta tctataaatc
      361 ccttcggtta acaccgaaaa gaaaaactcc cattgatcca gatatacctc acgcgctgca
      421 tcaccttcct gatgagcata ataggcctcg gtaaaatcat tgcgtgtttt tatcaacaac
      481 catttcaaca agggaataat gcggtctttg tcgaatctaa gctcggtaaa tgaggcatcc
      541 tcatacagtc gatgaaagta actgtcaaag atctcgtttg tccgggtacg aaccgcccct
      601 tgctccacac gggatgagtt aaccataaag gctattattt cgggtttaag agagtaaaac
      661 tcaacagcga tataggcgat cagacgcgtt ctcatcaccg gatcggggga aaatccagct
      721 ggttccttgc gtgagacaat aaaatcatac agggcattgt aggaataccg gacaagatac
      781 tcgtacaaat catctttcga ttcgatgtac tcatacaagc cgcccttaga aattcctgct
      841 tccgccacaa tatcatccac actggttgcc tcaaaaccgg tgcgggcaaa acagataagg
      901 gctgcgtgaa gtacacgctc tcgtttgtct tcttttagat taaaaaaggt atctttcatt
      961 gatgttccat aaaaaaaacc gactacatag tcggttttaa gtagggtaaa cccgcaaatg
     1021 cgggtttgtc aatgaaaaga atgtcaggcc aggatattat taaccgcgat cttttggata
     1081 aaggctgctc gcatcaactc ttccattgta aaaaagccgg ttgtttcctt ttgtagggca
     1141 cgggcggcaa aaagagctcc ggcgccaaag gccccacgag atataacatc atgggtaatg
     1201 cgaatagttt ggttttgcaa cccaaagaca acctcgtgtt taccaacaat cccgccaaca
     1261 cgtatagaat tgatatgttt atccggatca agtcccagtt tctctgcaat tttgagcgct
     1321 gaaccactct ttcccgattt ttccctaaaa tgctcctcga ttacctcgat atccgcattg
     1381 ggaacaatct gctgaagaat ttgcgaagca acaagcaaga agttaatacc caaagttatg
     1441 tttgctgaat ataaaacggc tgttttctcc ccaagttgcc gtaaaaaatc acgttctttt
     1501 ttctcataat gcgagatagc agacacaata tgtatcccgc ggtctgcggc atgagcgtat
     1561 tcatagacgc cctgtgtgga agagaaatcg atgataacat caacggggtt attcttaaaa
     1621 aaatccgtat ccccaacctc atccatacaa aaaaaatagc cctgtttatt atgcggatag
     1681 cctaaaaaat cgctggcata ttcatgattg ttttgcgccg atttacgaac tacccatgcc
     1741 aggttacaat cagggtcatg aataatctct tcggcaacaa gccttcctgt acgtccaaaa
     1801 ccaaataatc ctactttcaa atactctacc tcctaaagtt gtatagcaaa atgtaagcat
     1861 aagttgatag ctctacagag tcaagcaagg ttttatgggt atcattggag aaaacgacag
     1921 tttttcctcc tttttttggg taaatcctga aaaatctggt atagtatgta caggagtgtc
     1981 tttcgttcat gaaatcaagg gcgagcatcc gcaagcaggc actgcctacc attgcggaat
     2041 caaaccgcac agttgcacgt atactggatc tcctccagca aaaagataca tttttgctcc
     2101 ttggacacga attccccgat gaggattgtg tcgcgtcact tgtatcaatg gccctgatta
     2161 tccacaagtg tggaaaaaag gtgtcgctct accttcaaaa tgaaattccc ggccaattat
     2221 cctttctcgc cggcatctgc gcctataaca agatacccct gctccaggga gacgacaaac
     2281 ctgcagagca tcctcaggta atcggcattc tggacacgcc aaagcccgat atgatcgccc
     2341 tgaattctgc catttccggc tacctggcct cccctgatat tcccattttc gagtttgatc
     2401 atcacttatc agctgatgcc caatatagcg ggaggcctgg cctgtgtctg gtaaatcgcg
     2461 cttccagcac ctgcgagctc ataggctttt tttgcgcaaa actctcacgc cgcccggata
     2521 tccttgcaac cctgggcata cgaaatctgt actcccgcaa cctggtgctt tctctgctca
     2581 ccgggatgat cgccgatacc aaatttggta ttacccttaa aaccgatcgg gaccgtttct
     2641 tctataatta cttcaccaat cgctttgcca gtatcctaag ggatagcaac tataagaaca
     2701 gcgggaacta ctcttccctg acggacatct ttaagtcaat ccaattactg acggtaaatg
     2761 agcgggatgt atatcttcgg ttactgtccc ataccaaagt tcttggcaag gtagcctata
     2821 ttgcccttga ttacgaaaat tcccacaact atctaacccg tcttgaacca gggctttttg
     2881 tcaaggtaat caaatccgtt accgacgaac tgtgtgacaa gtcagagggg atcgggctta
     2941 ccgcttatta tgacatgagc gaaacctcgg atctgattca atttcgtatt agaacctcca
     3001 aaagcataac gaatatagat ttacgcacca tcttgaccga gttttcaatc actgatggag
     3061 ggggacaccc gggcgcaatc ggctttagaa tccccaaaaa tcaggtatcc gacatccata
     3121 cgtatattat ccccttgctt gaaaggctca acacgctatg agctaccgtc tctatacaga
     3181 caccctctcg cgcattggcc aac
//
LOCUS       DAOQXQ010000121         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_31901_length_3201_cov_2.487285, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000121 DAOQXQ010000000
VERSION     DAOQXQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31901_length_3201_cov_2.487285"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2231)
                     /locus_tag="PKH81_02790"
     CDS             complement(48..2231)
                     /locus_tag="PKH81_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNQ96995.1"
                     /translation="MPKPAWNLEENPKNKRWYVMIPEGLSEEHYPTIASIREKTREKG
                     IDVHTLLSESALEKSITKALSIPGEEFSFPAVIEPTFDVRLIIASDKTRASLYIRKAS
                     DNDRSMDMKLISTVINNSHLKGLDAQRIKDGIQAFKESAEMELPDFLLTEGKPPTRGK
                     DRELITQVQWHGEDETAKLRTRLVQANVLQPISPQDITHTAIVEKAQLLFEISPAETG
                     EDGEDVYGKPIPGLPGNDPFIQTLASISMGPFGLRSDADGLLIASINAKGVKAAVISY
                     KDAKATAVISADNMLASLILEKNVGPGIPLDIRLAEKALLDKGLTGTVNRKAIEEAIT
                     EVNTSGQSSEIVIIRGEKPVPSGGVRITIHAALNPQKNTAIVKAGDSILTLTSYPKGA
                     DGVDIHGTVLPLSQAIVEPLPTHDESIQEETSGNETIYRAVISGELTHHAQQWSISDT
                     KEMVCDITEETGDITFPSNLTLTGTITSGRAVKAGGNLTVNGTAEASLVYAEKNVTMN
                     GGIKGSGRGTVWSKDNMSLTFAENARLLSGGDIHISKYCFQCTIKTNGTLIIQGNPAL
                     LIGGSIRATKGVETFELGSEKTIRTSISFGQNYLIGDQIEVCEREALKIKETVAKIDE
                     MMKQTSNTNPKIHELRRKKLELLKRNDKLTVRIFTLKEQFETHVISHVRVENTVYPGV
                     ILESHGRYFEVKERLNHVIFIFDQKTGQITVNPIQDAPVASATQE"
     gene            complement(2247..>3201)
                     /locus_tag="PKH81_02795"
     CDS             complement(2247..>3201)
                     /locus_tag="PKH81_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNQ96996.1"
                     /translation="LTGASTFEASILETSWKGVSFLAGDSEIPGLTSLRAPQKASLTR
                     NLHKADADYLILDLGAGTHTAILDFFLQSTRGIVVTAPSVTATLNAYLFLKNAVFRLM
                     YSTFKKGSPAHTWLEGLKNDSPALQRMYIPRIIDEISRVDPDNAAIFRQKMSRFQPRL
                     VMNMIDDPKDADKALKIRRSCKEYLNLELEHLGVIYRDSIQDTALASRLPVIVYKPQS
                     ILSQAVYRIADKLLQAETEENEDLSELTEESFQTAEMEAAIDFDSRMNYVEELVGSGA
                     LTMGDLAETIKAQQYEIGVLKKENNLLKTKITQALMQGYTL"
BASE COUNT          695 a          709 c          837 g          960 t
ORIGIN      
        1 tatttccggg cttatccagg agggataatt gatatacatg aacatggtta ctcctgagtc
       61 gcagacgcta ccggggcgtc ttgtatgggg tttacggtta tttgtcccgt tttttgatcg
      121 aatataaaga taacatgatt aagccgttct ttaacttcaa aatagcgtcc gtggctttcc
      181 aggattacgc caggatacac cgtgttttct acacgcacat gggagataac gtgggtttca
      241 aactgttctt taagggtaaa aatacgaacc gtgagcttgt cgtttcgttt aagcagttca
      301 agtttcttcc tgcgcaattc gtgtatcttg ggattggtgt tggaggtttg cttcatcatc
      361 tcgtcaatct tggctactgt ttcttttatc ttgagcgctt cacgttcgca cacctcaatt
      421 tgatcgccaa tcaggtagtt ctgtccaaac gaaatactgg tccggatggt tttttccgat
      481 cctagttcaa aggtttctac tccctttgtc gcgcggattg accctcctat caagagggcg
      541 gggttgcctt gtatgataag ggttccattt gttttgatgg tacactgaaa gcaatatttg
      601 ctgatatgaa tgtcccctcc tgaaaggaga cgcgcatttt cggcgaaggt aaggctcatg
      661 ttatccttgg accatactgt tcctctgcca cttcctttaa taccaccatt catggtcaca
      721 tttttttcgg cataaaccag agaggcttct gcggtaccat tgacagtaag gtttcctccc
      781 gcctttactg ctcgtccgga ggtaattgtg ccggtaagcg taaggttgga ggggaatgtt
      841 atatcccctg tttcttcggt aatgtcgcac accatttctt tggtgtccga gatactccac
      901 tgctgtgcgt gatgggttaa ttcccccgag ataacagcgc gatagatggt ttcgttgccg
      961 gacgtttctt cttgtatgga ttcatcatga gtaggtagcg gttccacgat tgcttgtgaa
     1021 aggggtaata cagtgccgtg aatatccaca ccgtctgcgc ccttgggata gctggtaagg
     1081 gtaaggattg aatctcctgc cttaacgatt gccgtgtttt tttgagggtt caaggctgca
     1141 tggatggtta ttcgaacccc accgctcggg acgggttttt ctccacgaat gatgacgatc
     1201 tccgaacttt gtccgcttgt gtttacttct gtaatggctt cttcaattgc ctttcggttc
     1261 actgtaccgg taagaccctt gtccagcaaa gctttttctg caagccgtat atcaagcggg
     1321 attccaggtc caacattctt ttcaagtata agagaggcaa gcatattgtc ggcggagata
     1381 accgcggttg cctttgcgtc tttataggat ataacagcgg ccttaacgcc cttggcatta
     1441 attgaggcaa tcagcaagcc gtcggcatct gagcgcaggc caaagggtcc catggaaata
     1501 gaggcaaggg tttgaataaa aggatcattc ccgggaagtc ctggtatggg tttcccgtag
     1561 acatcttctc cgtcttctcc tgtttccgcc ggacttatct cgaacaggag ctgtgctttt
     1621 tccacgattg ctgtatgagt aatgtcctga gggctaattg gctggaggac atttgcttga
     1681 acaagccgtg ttctgagttt ggctgtttcg tcttctccat gccactgtac ttgggtgatc
     1741 agttcccgat ctttgcctcg tgtgggaggc ttgccctcgg ttaagaggaa gtcgggcagt
     1801 tccatttccg ctgactcctt aaaggcttgt atgccgtcct tgatgcgttg cgcatcaagg
     1861 cctttaaggt gactattatt aataacggta gatatcagct tcatgtccat gcttcgatcg
     1921 ttgtccgagg ccttgcgtat atagagcgag gcccgggttt tgtcggaagc gattatcagg
     1981 cggacatcaa aagttggctc aataacagca gggaaggaaa actcttcgcc aggaatggag
     2041 agggccttgg taatactttt ttccagggcg gattcggata agagtgtatg tacatcgatg
     2101 cccttctcac gggtcttttc gcggatcgag gcgatggttg gatagtgttc ctccgaaagg
     2161 ccctcgggaa tcataacgta ccatcgtttg ttttttggat tttcttccag gttccaggcg
     2221 ggttttggca tagtgcgctt accggatcaa agggtgtaac cctgcatcaa ggcctgggta
     2281 atcttggttt ttaataggtt gttttctttt ttaagaacgc ctatctcgta ttgctgggct
     2341 tttatggtct ctgcaagatc tcccatcgta agggcgccag agccaaccag ttcttcaaca
     2401 taattcattc gggaatcaaa atcgatggcg gcttccattt cggccgtttg aaaactctct
     2461 tcggtaagtt cagaaagatc ttcgttttcc tcggtttctg cctgaaggag cttgtcggct
     2521 atgcgatata ccgcctggga aagaatggac tggggtttat agacgattac tgggagccgg
     2581 gatgccaagg ctgtatcttg aatcgagtct cggtaaataa cacctagatg ttcaagctcc
     2641 agattaaggt actccttgca ggatcgccta atcttgagcg ccttgtccgc atctttggga
     2701 tcgtcaatca tgttcatcac cagccggggt tgaaagcggc tcattttctg gcggaagatg
     2761 gccgcattat ccgggtctac ccggcttatt tcgtcgataa ttctgggaat atacatgcgt
     2821 tgcagggcag ggctgtcgtt tttgagccct tctagccagg tgtgagccgg ggagcccttt
     2881 ttgaatgttg agtacataag gcggaatacc gcgttcttca gaaagagata tgcgttgagt
     2941 gttgcggtaa ccgaaggagc ggttactact atgcctcggg ttgattgtaa aaagaagtca
     3001 aggattgcgg tatgcgttcc ggctcccaga tcaaggataa ggtagtcggc gtcggccttg
     3061 tggaggtttc tggtcaaaga ggctttttgt ggggcgcgca gggaggtaag cccgggaatt
     3121 tccgaatctc ctgcaagaaa ggacacgccc ttccaggagg tttccagaat ggaagcttca
     3181 aaggttgaag caccggttaa a
//
LOCUS       DAOQXQ010000122         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32070_length_3189_cov_2.837907, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000122 DAOQXQ010000000
VERSION     DAOQXQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32070_length_3189_cov_2.837907"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKH81_02800"
     CDS             <1..309
                     /locus_tag="PKH81_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ96997.1"
                     /translation="TVRYVHRGTAYELTTDKKGSFDLLTGAKMASGLADNVGGKFVLK
                     AEFVKDGTDGTKMADLDWSKITLVPDTKAADASRYYEGTLSFTYKGGMLKVKGNLVQK
                     "
     gene            complement(358..443)
                     /locus_tag="PKH81_02805"
     tRNA            complement(358..443)
                     /locus_tag="PKH81_02805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Leu,seq:taa)
     gene            485..1345
                     /locus_tag="PKH81_02810"
     CDS             485..1345
                     /locus_tag="PKH81_02810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ96998.1"
                     /translation="MYADTHCHLSLIAERGITLSELLLELEEKQFQFVMDIGTTPGDL
                     GKRVETVLSSFPSIPSYLFFSCGLWPSPQSIAEADESIAALERDLDTLLPTDALPDYA
                     ALGECGIDRHWNGKPPVPGGEGGSLDLSGEEDLFARQIAIARSRNLPVIVHSREAFDI
                     TRSIIANEGWSRGVIHCFSYGKTEARSFLDLGYYISFPATITWGKKSVDRDRIAELLS
                     YVPQDRLLLETDAPYMAPVPHRGEINTPARIPLVYQSAALFLGITEEALAARVVHNAR
                     TLFAITKGLC"
     gene            complement(1326..3155)
                     /locus_tag="PKH81_02815"
     CDS             complement(1326..3155)
                     /locus_tag="PKH81_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNQ96999.1"
                     /translation="MLKKTVNLFALALLAMLTVFPLLFRLLSYFSGIVIPSTDLALVN
                     MVFMFTGFAGIITSSANRHLNLGAINDALPPRAKRVINPLRASITAAVLTALFLSAFS
                     ELFMIFESDERLWGVPLKAIFSILPIMFFAMTAIHIKRSANKIAALIGSLIGIFIATG
                     PIVGIAYFLFKIESLVAFQAAFDLWLHASHVLLWPLVILLILSAVFGVSIFVVLSGIA
                     YICFSQGGGYVEMIPLEYYQILTDKSIAAVPLFTLAGYLLAEGSAGKRLLDVVKNSVG
                     WIKGGPVIAAVLVATFFTTFTGASGVTILALGGLLTVILTGSGYKKEEAESLVTASGS
                     IGMLFPPSLAIIIFGTTNIFSVDIFELFKGALIPGFLMAVSMIFLGILRDRTVHRSPF
                     SPRELAKALYHGAAEIFLPICIVAGYFSGFLSLIETAAFAAAYAFILEVLIRRDYTVK
                     QAGKIILKSVPVAGGVLVIIGAAKGLAFFLVDAGIPFILTDFVLTFVQSKYLFLFLLN
                     IMLLAVGCLMDLYSAILVVSPLIMPVAESFGIHPVHTGVIFLTNLALGFLTPPVGMNL
                     FIASYTFNKPIMKLVKYIIPYLIVQFIILMLVTYIPWFSTALL"
BASE COUNT          916 a          699 c          800 g          774 t
ORIGIN      
        1 accgttcgct acgtacaccg tggtaccgcc tatgaattaa ctacagacaa aaagggatct
       61 tttgacttgc tcactggagc aaagatggct tccggtttgg ctgataatgt tggtggcaaa
      121 ttcgttctta aggctgagtt tgttaaagac ggaacagacg gtactaagat ggctgacctt
      181 gattggtcca agattactct tgttcccgat accaaggcag ctgatgctag caggtattat
      241 gaaggcacat tgtcctttac ctacaagggc gggatgctga aggttaaagg aaaccttgtt
      301 cagaaataag gtatgtcttt atgaataaaa aacgcgccgt aaggcgcgtt ttttatttgc
      361 cggggaccgg gattgaaccg gcacggccgt ttccagccaa gggattttaa gtccctggtg
      421 tctaccaatt ccaccacccc ggcgctgtat gctatagtac cgaaacaagg ggatgaggtc
      481 aataatgtac gcggatactc actgtcattt atcactaatt gcggaacggg gaattacact
      541 tagcgagttg ttactggaac tggaagaaaa acaattccag tttgttatgg atataggcac
      601 tacaccggga gaccttggaa agcgagttga gactgttcta tcttctttcc cgtctattcc
      661 atcctatctt tttttctcct gcgggctgtg gccctctccc caatcaattg ctgaagcaga
      721 cgagtctatc gcggctcttg agcgagattt ggatacactc ttgccaacag atgctcttcc
      781 agactacgcg gcccttgggg aatgcggtat agaccgtcac tggaatggta aacctccggt
      841 tccgggaggg gagggaggct ctcttgatct gagcggcgag gaagaccttt ttgcccgtca
      901 aatagctatt gcccgatcaa gaaacttgcc agtaattgtt cattcaagag aagcctttga
      961 tattactcga tccattatcg caaacgaggg atggtcacga ggcgttattc attgtttttc
     1021 gtatggtaaa acggaggctc gctctttttt ggatctgggc tattatattt cttttcccgc
     1081 aacgattacc tgggggaaaa aaagcgtaga tagagaccgt atcgctgaac ttctttctta
     1141 tgtacctcaa gatagactac tccttgaaac tgatgcgccc tacatggcgc cagtcccgca
     1201 tcgtggagaa ataaatacgc ctgcgcgtat tccgcttgtc tatcaaagtg cagctctgtt
     1261 tttggggata accgaagaag cccttgccgc gcgcgttgtt cacaacgcgc gcactctctt
     1321 tgctattaca aaagggctgt gctgaaccag ggaatgtagg ttaccaacat caggataata
     1381 aactgaacga taagataggg aatgatgtac tttaccaact tcataattgg cttgttaaat
     1441 gtgtagcttg ctataaagag gttcatgccg actggagggg ttaaaaaacc caacgccaaa
     1501 ttggtcagga agataactcc ggtgtgtacc gggtgtatgc caaaggattc ggctaccggc
     1561 atgataagcg gagataccac aaggattgct gagtacagat ccattaaaca gccaacggct
     1621 aacaacatga tattaagcag gaacaagaac agatactttg attgaacaaa agttaataca
     1681 aagtcggtga gtataaaggg gattcctgcg tctacaagga aaaaggcaag acccttggcg
     1741 gctccaataa taacaaggac gcccccggca accggaacac tttttaagat tatctttccg
     1801 gcttgcttga cggtataatc ccttcggata agaacttcca gaataaaggc ataggcggca
     1861 gcaaaggcgg ctgtttcgat aagagacaaa aagccgctaa aataaccggc tacaatgcat
     1921 attggaagga aaatctctgc agccccatgg tagagcgcct tggcaagctc ccggggagaa
     1981 aagggggagc gatggacggt gcggtcccgg agtattccca aaaaaatcat ggagacagcc
     2041 attaaaaagc cggggataag cgcgcctttg aataactcaa agatgtctac agaaaagata
     2101 ttggtagttc caaagataat aatggcgagg cttgggggga acaacatgcc tatggaacca
     2161 gaggcggtaa ccagggattc agcttcctct tttttataac cgcttccggt aaggattact
     2221 gttaataagc ctccaagggc gagtatggta acgcctgaag caccggtaaa ggtggtaaaa
     2281 aaggttgcca ccaatactgc cgcgataacc ggtccgcctt taatccagcc aacgctgttt
     2341 tttactacgt ccagtaagcg cttgcccgcg cttccttcgg caagaaggta tcctgcaagg
     2401 gtaaatagcg gaaccgcggc aatgcttttg tcggtaagga tctgataata ttccaggggg
     2461 atcatctcga cgtatccgcc accttggcta aagcagatat atgcaatacc gctgagcact
     2521 acaaagatgg acacgccgaa gaccgccgac aggataagga gtattaccag tggccacaac
     2581 aagacgtgag atgcgtgaag ccacaggtca aaggctgctt gaaacgcaac aagggattca
     2641 atcttgaaaa gaaaataggc aattccgaca atgggtccgg tagcgatgaa tataccaatc
     2701 agcgatccga ttagggctgc gatcttgttg gcgcttcttt taatgtggat cgccgtcatg
     2761 gcaaaaaaca ttattggaag gatggaaaaa atagccttaa gcggaacacc ccacagtcgt
     2821 tcgtcgcttt caaagatcat aaataattcg gagaaagcgg aaaggaagag tgcggttaat
     2881 accgccgcgg taatcgatgc ccgaagggga ttgataactc tttttgcccg tggaggtaat
     2941 gcatcattta tggctcccag gtttaaatga cggttagcac tgctggtaat gattcctgca
     3001 aaaccggtaa acataaagac catgtttacc agagcaaggt cggtggatgg tataacaatc
     3061 ccgctgaaat aagaaagcaa gcggaagagc aggggaaata cggtaagcat cgccaacaga
     3121 gcaagggcaa aaaggttgac cgttttcttt aacatcactt atttccttgt ctctgcaagc
     3181 agtctttgt
//
LOCUS       DAOQXQ010000123         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32193_length_3180_cov_2.925760, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000123 DAOQXQ010000000
VERSION     DAOQXQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32193_length_3180_cov_2.925760"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKH81_02820"
     CDS             complement(<1..1045)
                     /locus_tag="PKH81_02820"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNQ97000.1"
                     /translation="MVILTLNCGSSSAKYQVFDWDHRDVIASGVVERVTQEGGSITHN
                     AKGKPEYKISHECPNHTIAVELIIHTLTDPEVGVIKDMSVIRAVGHRVLHGGNKFTKS
                     VVVTPEILETFREVQDLGPLHNPANIMGIEAAQKVLPNVPHCAIMDTAWHQTMPDTSY
                     MYAVPYEWYTDYAVRRYGFHGTSFLYTAKRASVVLGKKPEDTNVIIAHIGNGASMCCV
                     KNGKCWDTTMGLTPLEGLVMGTRSGDCDPALAFYMMRKADMSVQDIDTALNKKSGLLG
                     ITGKYADRRDVTKAMDAGDKRAQLAYNIECYRLRKYIGSYIAAMGQLPDALIFTAGVG
                     EFHAGVRKGVLSGL"
     gene            1206..1973
                     /locus_tag="PKH81_02825"
     CDS             1206..1973
                     /locus_tag="PKH81_02825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ97001.1"
                     /translation="MEKKRDWFENESFWAQYAPVLFDAQRWAEAPSVAEGILHIIGAA
                     NVEGKEGPAILDAGCGPGRISVELAARGARVTGVDIIRPFLNAALESAQDEGVELELV
                     QADLRTFSRPGAFDAAVSLYTSFGYCDTIEEDMTILRNIASALVPGGWFILEMTGREI
                     AVRDFTEGEWFERGGFTVLTEFSVEGAWEGLRSRWMLIDENGRKIDHVFVQRLYSAVE
                     LKRLMMATGFSSVEIYGDFDFSPYNEKARTMVLVART"
     gene            complement(2059..>3180)
                     /locus_tag="PKH81_02830"
     CDS             complement(2059..>3180)
                     /locus_tag="PKH81_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ97002.1"
                     /translation="ESTDRAGNKTRTQLAGIIVDSRVPKAFITAEHAAFSPNADGLKD
                     VQKLSLVTNLTTGLSSWQVSIKAGSSASAPIKTWSSKDQATLPASINWDGKDSSGKIA
                     QGSFIAELTLTYEKGDTVTTATPAFLASTEPPALGVQLSPKYFSPDNDGMEDELFINL
                     QAKSDAAFAQWSFEIREPEGTAGNVFWRTGGTNRITERVIWDGRSLKGELVQAATDYP
                     YTFTVKDELGMTSVVRGYIPVDVLVIREGDRLKIAVPSIIFRENAADFNGLDPAVVEK
                     NTQVLKRIAEILNKFRDYRIQVEGHANNVTGTQKEEDTELIPLSRLRSDAVRDFLIRN
                     GVDGSRLTTVGLGGTRPVVQRQDRENWWKNRRVEFVLIK"
BASE COUNT          687 a          752 c          918 g          823 t
ORIGIN      
        1 caagaccgct taacacgcct tttcttaccc ctgcgtggaa ttcgccaact cccgcggtaa
       61 agataagcgc gtcgggaagt tgtcccatcg cggcgatata tgatccaatg tattttctaa
      121 ggcgatagca ttcgatgttg taggcaagct gagcccgctt gtctcccgca tccattgcct
      181 tggttacgtc ccgtcgatcg gcatacttgc cggtaatacc aagaagaccc gattttttat
      241 ttaaggcggt atcaatgtcc tgaacgctca tatcggcttt acgcatcata taaaacgcaa
      301 gggcagggtc gcagtcgccc gatcgggtgc ccataacaag accttcaagc ggggtaagac
      361 ccatggtggt atcccaacac ttaccgtttt ttacgcagca catggaggca ccgttgccga
      421 tatgcgcgat gataacgttc gtgtcctcgg gttttttacc aagaacaacc gaggcgcgtt
      481 ttgcggtgta caggaagctt gttccgtgga agccatagcg gcggacggcg tagtcggtat
      541 accattcgta gggaacggcg tacatatagc tggtatccgg catggtttgg tgccaggcgg
      601 tgtccataat ggcgcagtgg ggtacattgg gaagtacctt ttgggctgcc tctattccca
      661 tgatatttgc cgggttgtgg aggggcccta agtcctggac ttcccggaag gtttccagga
      721 tttccggggt aacaacaacc gatttggtga atttattgcc gccgtgcagc acccgatgtc
      781 ctacagcgcg aatgacgctc atatccttaa taaccccaac ttcgggatcg gtaagggtgt
      841 gaatgataag ctcaaccgcg atggtgtgat tggggcattc atggcttatt ttgtattctg
      901 gcttgccctt ggcgttatgg gtgatggacc cgccctcctg ggtaacccgt tcaacaacgc
      961 cggaggcgat gacgtcccgg tgatcccagt caaaaacttg atactttgcg gatgaactgc
     1021 cgcaattgag ggtaagaatg accatactaa ctcctgatta ttgtgtgcgc tttatgcgct
     1081 gtttcgtata agagccagtg taaacctatt aaaggtctct ttcaaggact cggtatttcc
     1141 ctggtggcgg ggacgggtgt tatgtagtaa aatacggcca gatcacgaat aaggaaggcg
     1201 atgagatgga aaaaaaacga gactggttcg agaacgagtc tttttgggcc cagtatgccc
     1261 cggtattatt cgacgcgcag cgctgggctg aggctccatc ggtcgcggag ggaatattgc
     1321 atatcattgg cgctgcaaac gttgaaggaa aagaaggtcc ggctatcctt gacgcaggct
     1381 gcggcccggg gcgaatttcc gttgagcttg ctgcccgcgg agcccgggta accggagtgg
     1441 atattatcag gccctttttg aatgcggccc ttgaaagcgc ccaggacgag ggggtggaat
     1501 tggagttggt gcaagctgac ctgagaacct tttccagacc gggtgccttt gacgctgcgg
     1561 tgagccttta tacgagtttt gggtactgcg ataccatcga agaggatatg acgattctta
     1621 gaaatattgc ttccgctctg gttcctggcg gttggtttat tcttgagatg accggtcgcg
     1681 aaatcgcggt acgggacttt accgagggtg aatggtttga aaggggcggg tttactgtct
     1741 taaccgagtt ttcggtggaa ggagcctggg aggggttgcg ttcgcgatgg atgcttattg
     1801 atgaaaatgg gcgcaagatt gaccatgtat ttgtgcagcg gctgtattct gccgttgaac
     1861 taaaacggct tatgatggca acgggctttt cttcggtgga gatatatggc gactttgatt
     1921 tttcacctta taatgaaaaa gccaggacga tggtgttagt ggcacgaacg taaaaaaaac
     1981 cgcccgtgca aaacgcgggc ggtttggaag gatgggcgcg ggaaggaaac cttataaaag
     2041 gttcccttcc cgtttttttt atttgattaa cacaaactca acacggcgat ttttccacca
     2101 gttttcgcga tcctggcgtt gaaccacggg acgagtgccg ccaaggccaa cggtggtaag
     2161 ccgtgaaccg tctacaccgt tacggatcag gaagtccctg accgcgtcgg agcgcaggcg
     2221 ggaaagcgga atcagttcgg tgtcctcttc cttttgcgtt ccggtaacat tgttagcgtg
     2281 tccttctacc tggatccggt aatcgcggaa cttgttcagg atttcggcaa tacgcttgag
     2341 tacctgggtg ttcttttcca cgacagccgg gtcaaggccg ttaaagtcag cggcgttttc
     2401 gcggaaaatt atggaaggta cggcgatctt gagcctgtct ccttcgcgga taaccaaaac
     2461 gtcaaccgga atgtagccgc gcacaacgct ggtcatgcca agctcgtcct taacggtaaa
     2521 ggtgtacgga tagtcggtcg cagcctgtac cagctctccc ttaagtgagc ggccgtccca
     2581 gatgacgcgt tcggtaatgc ggtttgttcc tccggttctc cagaatacgt ttccggcagt
     2641 tccttccggt tcgcgaatct cgaacgacca ttgcgcgaag gctgcatcgg actttgcctg
     2701 tagattgata aacagttcat cctccatacc atcattgtcc gggctgaagt acttgggact
     2761 tagctgtaca cccagtgctg gaggctctgt ggaggcaagg aaggcggggg ttgctgttgt
     2821 taccgtgtct cccttttcgt aggtaagggt gagttctgcg ataaagcttc cttgcgcgat
     2881 ttttcccgag ctgtctttgc catcccagtt aattgacgca ggcagggttg cctggtcttt
     2941 ggagctccat gttttaatgg gggcggaagc gctgcttcct gccttgatgg acacctgcca
     3001 tgaggaaaga ccggtggtaa ggtttgtgac cagggacaac ttttgtacgt ccttaaggcc
     3061 atctgcgttg ggagagaatg cggcatgttc ggcggtaata aaggccttgg gaacacggct
     3121 gtcaacaatg attcccgcaa gttgcgttcg ggtcttattc cccgcgcggt cggtgctttc
//
LOCUS       DAOQXQ010000124         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32273_length_3175_cov_2.967628, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000124 DAOQXQ010000000
VERSION     DAOQXQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32273_length_3175_cov_2.967628"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKH81_02835"
     CDS             <1..202
                     /locus_tag="PKH81_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97003.1"
                     /translation="FQTNRRVLLAETAKQESGRSLTEYMELERLAKVGELLTKYPILI
                     EYLAISGKDSAQAIETLKALRK"
     gene            complement(211..876)
                     /locus_tag="PKH81_02840"
     CDS             complement(211..876)
                     /locus_tag="PKH81_02840"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNQ97004.1"
                     /translation="MRAVLFIGGDQPDPNSCSPYIGNVDIVVAADSGFDGARRSGFSV
                     DYVIGDMDSIADPKVLEDFDDSRVIRWPRDKDYTDTECALEYLSGKGIIDIVLVGGTG
                     GRLDHLYAIQHLFTRPVCPWLWVGSETVVVPVGAGCAGNSIRLEGLRVDNPVSVFPAS
                     NAPLAVTSRGLYWELDSLDWSNGAYSLSNRSVASQAVISVTQGRFLVMFPLYPSITLV
                     WEG"
     gene            926..2281
                     /locus_tag="PKH81_02845"
     CDS             926..2281
                     /locus_tag="PKH81_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNQ97005.1"
                     /translation="MDRIPVPAVLKDLSRIFTAQGYQVYLVGGAVRDYFLGKKAEDWD
                     IATDAKAEEVATLFKRIIPTGIEHGTVTIPFRGHMIECTTFRIETGYTDSRHPDSVSF
                     TATITEDLSRRDFTMNAIAAALPTGEIVDPFDGRKDITKRIIRTVGDPHTRFSEDGLR
                     PLRAIRFSAQTGFTIEGPTLEAISPSIPIIKTVARERIRDELSKLLTSDNPLPALYYL
                     EQTGLLEHLIGELSMCRGVFQGGRHRHDVLDHLFYTCVYCPSASLELRLAGLFHDIGK
                     PVALAFDTEGNPTFHGHETVSASITEKIMTRLKFPGKTIKAVCHLIRQHMFHYESVWT
                     DAAVRRFIVRTGEDEIEQLFALRRADSRAISDIAEEGADIQELRERIIGILKQGHAFT
                     LKDLAINGHDLTSQGVPRGPVTGVILEELMQAVLDDPSLNNRERLLDISRAMCIERKL
                     I"
     gene            complement(2278..3018)
                     /gene="rnc"
                     /locus_tag="PKH81_02850"
     CDS             complement(2278..3018)
                     /gene="rnc"
                     /locus_tag="PKH81_02850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNQ97006.1"
                     /translation="MFGFRHTLTGERNQTLQAFQKRAGIRFKDSGLLDLALHHRSFSN
                     ENSKERVNNERLEFLGDAVLGMVVAAQLYECMADRPEGDLARTKSVVVSEDTLSRIAL
                     SLGISDYLVLGKGEEMSGGRTKKAILADALEAIIGALYLDSGYRTAEQFVLTLMKPEI
                     LLVLQNRHHKDYKTLLQEFVQKQYKTIPRYALVNRSGPDHDRTFSVTVEIHGQTYGPE
                     QGKNKKEAEQAVAQVACTALGVETQRES"
     gene            complement(3048..>3175)
                     /locus_tag="PKH81_02855"
     CDS             complement(3048..>3175)
                     /locus_tag="PKH81_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ97007.1"
                     /translation="LDTYELVYAIEEEMGISIPDEKANEFETVRDAYDFIKSQSK"
BASE COUNT          923 a          776 c          683 g          793 t
ORIGIN      
        1 atttcaaaca aaccgaaggg tgcttctggc cgaaacagcc aagcaggaat cgggtcggtc
       61 gctaactgag tatatggaac ttgaacggct tgcaaaagtt ggggagttgc ttactaaata
      121 tccgatattg attgaatact tggcaatcag cggaaaagac agcgcacaag cgatagaaac
      181 gcttaaggct ctacggaaat aaccacggca ttacccttcc cacacgagag taatacttgg
      241 atacaaagga aacataacca aaaaacgacc ctgtgtaacc gaaataaccg cctgagaagc
      301 gaccgatcgg ttactgaggc tgtatgctcc gtttgaccaa tcaagtgaat ccaattccca
      361 gtacaaaccg cgagaagtaa ctgccaaggg agcattcgaa gcaggaaaca cagacaccgg
      421 attatctacc cttaacccct ctagtcgtat cgaattgcca gcacaccccg caccaacggg
      481 aaccacaacg gtctcactgc caacccacaa ccaaggacac acgggacggg taaaaaggtg
      541 ctgaattgca taaagatgat caagacggcc accagtgccg ccaactaaca caatatcaat
      601 aattccttta ccagacagat actccaatgc acattcagtg tcggtataat ctttatcacg
      661 cggccatcga ataacccgtg aatcatcaaa atcttcaaga acctttggat ctgctatgct
      721 atccatatct ccaataacat aatcaacaga aaaaccagaa cgacgggctc catcaaatcc
      781 tgaatccgcg gcgacgacaa tatcaacatt accaatataa ggcgaacagg aattggggtc
      841 gggttgatca ccaccaataa ataaaactgc gcgcatacat cctcgcttgt gttctagaag
      901 ttctttccag tatactccag cattgatgga cagaataccc gtacccgcag tacttaaaga
      961 tctttcccgt atttttaccg cacaaggata tcaagtatac cttgtaggag gagcggtacg
     1021 agattatttt ctggggaaaa aagcagaaga ctgggatatt gccacagatg ccaaggcaga
     1081 agaagtcgca accctattca aacggataat ccctactggg atagaacatg gaacggttac
     1141 cattcccttc cgtggacaca tgatcgaatg cactaccttt cgcattgaaa caggttacac
     1201 cgactcaaga caccccgatt cagtttcttt tactgcaaca ataaccgaag acctttctcg
     1261 ccgtgatttt accatgaatg cgattgcagc agccctaccc actggagaaa tcgttgatcc
     1321 ttttgatgga cgcaaagaca ttactaaacg cataataaga actgttggtg accctcacac
     1381 tcgcttttcc gaagacggat tgcgtccatt acgggcaatt cgcttctccg ctcaaacagg
     1441 gtttactatc gagggtccta cacttgaagc aatttcacct tccattccaa ttatcaaaac
     1501 ggttgcaaga gagaggatac gagacgagct cagtaaatta cttacatcag ataatccact
     1561 gccggccctg tactatctgg agcaaaccgg cttactagaa catttgatcg gtgagctttc
     1621 gatgtgccgg ggggtttttc agggtggacg acatcgccat gatgtacttg atcatctttt
     1681 ctatacctgt gtctattgtc cttcagcttc tcttgaactc agactggcag ggctttttca
     1741 tgacattgga aaacccgtgg ccctcgcctt tgacactgaa ggaaatccaa cttttcatgg
     1801 tcatgaaaca gtatccgcct caataaccga aaaaatcatg acgaggctca aattccccgg
     1861 gaaaactatc aaggcagtat gtcatttaat ccgtcaacac atgtttcact atgaaagtgt
     1921 ctggacagat gctgcggtca gacgctttat agtacggact ggagaggatg aaattgaaca
     1981 actgtttgct cttagacggg cagactcgcg tgcgataagc gatatcgctg aagaaggtgc
     2041 ggatattcaa gagctacgag agcgcattat tggaatactc aaacaaggtc atgcctttac
     2101 cctcaaggac ttggcaatta atggacatga cttaaccagc caaggcgttc ctcgcggacc
     2161 ggttaccggc gttatcctgg aagaactgat gcaagcagtg ttagatgatc cctctttgaa
     2221 taaccgggaa cgtttgcttg atattagtcg tgctatgtgt attgaacgaa aacttattta
     2281 ggactccctc tgagtttcta cccctagggc cgtacatgcc acctgagcca cggcctgttc
     2341 ggcttctttt ttgttttttc cctgctcagg tccataggtt tgtccgtgaa tttcaactgt
     2401 tactgaaaag gtccggtcat gatctggccc acttcgatta acaagagcat accgaggaat
     2461 ggttttatat tgtttttgaa caaactcctg caagagcgtt ttataatcct tgtgatgacg
     2521 gttttgtaac acaagcagaa tctcgggctt catcagggta agcacaaatt gttctgctgt
     2581 tctatagcct gaatcaagat ataaagcgcc gattatcgct tccagggcat cagcaagaat
     2641 tgcctttttt gtgcgtcctc cggacatttc ttcgcctttg cccaaaacaa ggtagtcgct
     2701 aatccccaag gaaagtgcta tacgagaaag agtgtcctcg gaaacaacaa cagatttcgt
     2761 gcgagccaaa tccccttcgg gacgatcagc catacactca tataattgag cggcgacaac
     2821 cattcccaaa acggcgtcgc ccaagaactc cagtcgctcg ttattgaccc gttctttgga
     2881 gttttcattg gaaaatgaac gatgatggag ggctaaatca agaaggccgg aatccttgaa
     2941 tcggataccg gcccgctttt gaaaggcttg aagcgtctgg ttccgttcac ccgttagtgt
     3001 atgacgaaac ccaaacaatg acgcctcctt gtgtatgtaa gaaaaattta cttggactga
     3061 gacttgataa aatcgtacgc gtcgcgaacg gtttcaaatt cgttggcttt ctcgtccgga
     3121 atactgattc ccatctcttc ttcgatggca tatacaagct cataggtgtc aagac
//
LOCUS       DAOQXQ010000125         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32298_length_3174_cov_2.462007, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000125 DAOQXQ010000000
VERSION     DAOQXQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32298_length_3174_cov_2.462007"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKH81_02860"
     CDS             complement(<1..394)
                     /locus_tag="PKH81_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNQ97008.1"
                     /translation="MKETEKWHAKTLGEAACAALKKNGFAALYMDSASQAADWIAAQV
                     TTGMSVGTGGSMTLASLAIHDRLEGMGARVLNHSRAGLTPDEKMEVMRAQLTSDLFLS
                     GCNAITLEGELYNIDGNGNRVAALTFGPK"
     gene            457..1377
                     /locus_tag="PKH81_02865"
     CDS             457..1377
                     /locus_tag="PKH81_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ97009.1"
                     /translation="MPKFCAIGLSSTIQETITFDSVAIGDVNRSKSYRLDASGKAVNA
                     ARVLGQIEKGSVSAVCPLGRENAALFLSLAAEDCIPVIPVMTPGKTRYCYTLLESSRG
                     RATELVVSEPVSSAASEARDFKRAADNILEILSGMSVTGAFIMAGSRPAAFPDNLYPR
                     LRSFAASRGVPVMVDFHGKDLLDTIAAGAPEVIKINEREFLSTFEPAHTEMRESDLAD
                     SIVRESSRLGSIIIVTRGARRVLAAYNGKVYREPVEPVEPVNEIGCGDAFSAGFIHTW
                     TATNDIQRALKRAVTCAAANLVTERPGSIK"
     gene            1451..2980
                     /locus_tag="PKH81_02870"
     CDS             1451..2980
                     /locus_tag="PKH81_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNQ97010.1"
                     /translation="MTRGFDNERYVAEQASFIRERMKRFDCRLYLEFGGKLLNDFHAS
                     RVLPGYDPNVKIRLLKELKDDAQIILCIHAGDIEHRKIRADYGITYDADAMKLIDDLG
                     EWNLSVGFVVITRWENQPAAKAFKIKLENRGVKVYCHTPTKGYPTDIDRIVSDEGYGA
                     NPRIETTKPLIVVTGPGPGSGKMATCLSQIYHDNKLGIHAMYAKFETFPIWSLPLKHP
                     VNLAYEAATLDLKDFNLIDPFHLDATGETAVNYNRDVESFPILRAILERITGSAEIYR
                     SPTEMGVNRAGFAIIDDEATREAAKQEIVRRYLRISCEFMMGMTDQDTVNRADRLLKE
                     LSIGVEYRPVLVAARKAAEEAFQSASRKKESESTVDVHCGAALELPSGEIVTGKNSRL
                     LHAATAAVLNGIKTLAGVPDNIQLLSPLVIEAVGNFKRKTMKGKSASLDVDEAMIALA
                     ISSALTPMVQTCLDHLHELRGCQMHLTHYPTPGDEAGLRRLGVNLTSDPVFASHRLFV
                     E"
BASE COUNT          734 a          792 c          826 g          822 t
ORIGIN      
        1 ttttaggtcc aaaggtaagc gctgcaacac ggtttccatt tccatctata ttgtacagtt
       61 ctccctccag ggtaatcgca ttgcacccgg aaagaaaaag atcgctggta agctgagcgc
      121 gcataacttc cattttttcg tcgggggtca ggccagctcg ggagtgattg agtactcggg
      181 cgcccattcc ctcaaggcga tcatgtatgg cgagcgaggc aagagtcatt gagccaccag
      241 tgcccacgct cataccggtg gtaacctgcg cggctatcca gtcggcggct tgtgaagcgc
      301 tgtccatgta cagagcggcg aatccatttt ttttaagcgc cgcgcacgca gcctcgccga
      361 gcgttttagc atgccatttt tcggtttctt tcatcttatt cctccctgtt gccaaattga
      421 tgcgtgtacc ttactctgaa gacgggggga aagtaaatgc cgaaattctg cgcaattgga
      481 ctgagcagta caattcaaga gaccattacc tttgacagtg tagccatagg cgatgttaat
      541 cgttcaaaaa gctatcgctt ggatgcctcc ggcaaggcgg ttaatgccgc acgcgtattg
      601 ggtcaaatcg agaaagggtc ggtatcggct gtctgtcccc ttggacgaga aaacgccgcc
      661 ctttttctgt ctctagccgc agaagactgt attccagtaa tcccggttat gactccggga
      721 aaaactcgtt attgttacac cctgctcgaa agttcccggg gacgggcgac ggagcttgtg
      781 gtaagcgagc ctgtgtcctc cgccgcttca gaggcccgcg attttaagcg tgccgcagat
      841 aatatcctgg agattctttc cggtatgtcg gtaaccgggg cctttatcat ggcaggaagc
      901 cgccccgcag cctttccgga caatttgtac ccccggcttc gctcctttgc cgctagcagg
      961 ggtgttccgg tgatggtgga ttttcatggt aaggacttac tcgatactat tgcagcgggc
     1021 gctccggagg ttatcaagat aaacgagcgg gaattccttt ctacgtttga gccggcacac
     1081 acagaaatgc gtgaatctga tttggccgat agtatagtac gggaaagtag ccgccttggt
     1141 tccatcatta tcgttacccg tggcgcccgt cgcgtcctgg ccgcttataa cggcaaggta
     1201 tatcgcgaac cggttgagcc ggtggaaccg gttaacgaaa ttggttgtgg agatgctttt
     1261 tccgcgggtt ttattcatac ctggacagcg actaacgata tccagcgtgc gcttaagcgc
     1321 gcagttacct gtgccgctgc taatttggta acggaacggc caggaagcat taaataatcg
     1381 ggattccctg ttgcaaaccc cggcccgtgc ggtatacact ccttttcgga ttacaaggga
     1441 gtaacagggt atgacacgcg gttttgataa tgaacgatat gtggctgagc aagcatcttt
     1501 tatccgtgag cggatgaagc gctttgattg tcgtctgtat ctggaatttg gcggaaagct
     1561 cttgaatgac tttcacgcat ctcgcgtcct tcccggttat gatccaaatg taaagattcg
     1621 cctgttaaaa gaacttaagg atgatgcaca gataattctg tgtattcatg caggggacat
     1681 tgaacatcgt aaaattcgcg ccgactatgg tattacctat gatgcagatg caatgaagct
     1741 tattgacgat ttgggagaat ggaacctctc tgtcggcttt gttgttatta cccgctggga
     1801 aaatcaacct gctgcaaagg cattcaagat aaaattggag aaccgtggtg ttaaggtgta
     1861 ttgccatact ccaaccaagg gctacccgac tgatatagac cgtatcgttt ccgatgaagg
     1921 ctatggagcg aatccccgaa tcgaaacgac taaaccgctt attgttgtaa ctggccctgg
     1981 cccgggttcg ggcaagatgg caacctgtct ttcgcagatc tatcacgaca ataagctggg
     2041 cattcatgcc atgtacgcca agttcgagac ctttccgatt tggtcacttc cgctcaagca
     2101 tccggtgaac ctcgcctacg aagcggctac cctggatctt aaagacttta atcttattga
     2161 tcccttccat ctagacgcca ctggagagac agcggtgaac tataaccgcg acgttgaatc
     2221 ctttcccatt ttacgcgcca tattggaacg tattaccggt tcagccgaga tttaccgctc
     2281 tcctaccgag atgggggtaa accgggccgg atttgccatt attgacgacg aagcgactcg
     2341 cgaggcagcc aaacaggaga ttgttcgccg gtatctcagg atttcctgcg agtttatgat
     2401 gggtatgacc gatcaggata ccgtaaatcg ggccgatcgc ttgcttaagg aattatccat
     2461 cggcgtggaa tatcggccgg tgcttgttgc cgcccgaaag gcagcagagg aagcattcca
     2521 gtctgcttcc cgaaaaaaag agagcgagtc cacagtcgat gtgcattgcg gtgcggctct
     2581 tgaattaccc tccggagaga ttgttaccgg caagaactca cgcctgcttc atgccgcaac
     2641 agcagctgtt cttaatggta ttaagaccct cgctggcgtc ccggacaata tacagctcct
     2701 ttctcctttg gttatcgagg cggttggaaa ttttaagcga aaaacgatga agggtaaaag
     2761 cgcgagtcta gatgtcgatg aggcgatgat tgcccttgcc atcagttcgg cgctgactcc
     2821 tatggttcaa acctgccttg atcacctcca tgaacttcgt gggtgccaga tgcaccttac
     2881 gcattacccc acccccggcg atgaagcagg tttgcgtagg cttggggtaa acctgacctc
     2941 tgatcccgtt ttcgcttctc atcgactatt tgtggaataa ttcgtcaagc gggcgtatac
     3001 taatccggta tggcagcgca ccggaaaaca cccttggcgg tattcctttt tttggtattg
     3061 ttctctgtgg ttttttccac gaaggtccgt gccgaaccaa ccattacctt tgagctacca
     3121 acaattattg gtggggatgg ggagatcgag cgatcgctcg aagcatccct ggaa
//
LOCUS       DAOQXQ010000126         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32316_length_3172_cov_3.463266, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000126 DAOQXQ010000000
VERSION     DAOQXQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32316_length_3172_cov_3.463266"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKH81_02875"
     CDS             complement(<1..523)
                     /locus_tag="PKH81_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ97011.1"
                     /translation="MKISELSVRKPVTTGMLYVLVCVIAGIFVPRLGVALYPSVTMPV
                     LSVFTTWTDVGPEEVEKNITRVLESRLSVVSGLESMTSTSSSGQSSIRLTFGYDVDLD
                     EALTDVKEVLTRAANVMPDEASVPTVRRFDISASPFMRLSVSGDVPVTELKVVADDTI
                     APLLERIKGVAQAD"
     gene            complement(520..1461)
                     /locus_tag="PKH81_02880"
     CDS             complement(520..1461)
                     /locus_tag="PKH81_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ97012.1"
                     /translation="MKDFERITKSILLALIGISTLALVLSLFLKAKDPQKAGGPQGKA
                     PRNGAATITVSVLEISASSLAQRVRLNGDVLSRSRIALYADTAGKLVSYEAGIGSEVK
                     KGDVVARIDPSRPGSPFAVSPVRSTIRGTIIALPYDPGDTISTSSPVATIGTLDDLEI
                     ITYVPEKYIAVLRTGLPASVSFVSWPGEEYPARVTMVSPVVETESRTVEIRLSVDAPK
                     DRLKHGMFAVISLVMREVKNAIVVPKTAIKTFNGESVVYVAGEDGLAQRRVVTTGLTS
                     DTETEIREGLSFAEKVIVSGSVSDGTPVRLANAGGKK"
     gene            complement(1448..2770)
                     /locus_tag="PKH81_02885"
     CDS             complement(1448..2770)
                     /locus_tag="PKH81_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ97013.1"
                     /translation="MSIVLKSKIFLVLTGLCVALGVHADTRDLSIAQAIELARLGNAS
                     LKKMRLERDIAVLKEKNAWNAFLPAIALSGSLRNTHSLGGSTTVPTGTEIGASAGLSL
                     QLQPAVRESLRQTALAREISELSYDALERDVLLSVHTAYYNLLADREGLALAEKDFQL
                     AGSQAVYVKKKYDNGLVSELSYLQAKYAADSLEPKLMDQRRSYRQAVRNFCALIALPP
                     DTDITLVDTLDHNFRPVTISCTLDDFLARRSDVRLALLNITLAQSARTGATLNRYGPT
                     VSLSENFQASGLQDELNMPETGTFTLSVSIPLNGYIPGSKERINAREKEAAVESATIA
                     FEQTLVSARNEALSQMDLLDQLYRSMEITRENERIASRAWNLSVQGFESGLVDQNDLD
                     SARQKMLNARLAVLRAGYAYKAGLLQLSAALGLDESELCGMEEAHEGL"
     gene            2999..3072
                     /locus_tag="PKH81_02890"
     tRNA            2999..3072
                     /locus_tag="PKH81_02890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3033..3035,aa:Ala,seq:ggc)
BASE COUNT          829 a          847 c          719 g          777 t
ORIGIN      
        1 catcagcttg ggcaaccccc ttaattctct ccagtaatgg agcgatggtg tcgtcagcta
       61 caaccttaag ttctgtaacc ggtacatcac cagaaactga aaggcgcata aacggagaag
      121 cgcttatatc aaagcgacgc acggtaggaa ccgaggcttc atcgggcatg acatttgccg
      181 cgcgcgttaa cacctctttt acatcagtaa gggcttcgtc aaggtcaacg tcgtatccaa
      241 aggtcagacg aatcgagctt tgacccgaag aagaagtaga ggtcatcgat tcaagccccg
      301 aaacaacgct taaccgggac tcaagtacac gagttatgtt tttttctact tcttccgggc
      361 ctacgtctgt ccacgttgta aatacagaca aaaccggcat tgttaccgac ggataaagtg
      421 caaccccgag ccggggcaca aatataccgg cgataacaca taccagcacg tataacatcc
      481 ccgtggttac cggctttcta accgacagct cagagatttt catttcttgc cacctgcgtt
      541 cgcaagccga actggagtcc catccgatac agagccagac actataacct tttccgcaaa
      601 agaaagtcct tcgcgaattt ctgtttccgt gtcgcttgtt aggcctgtag ttaccactct
      661 ccgttgagca aggccatctt cgcctgccac atatacaaca ctctctccgt taaaagtttt
      721 aatggcagtc ttgggcacaa cgattgcgtt ttttacttcc cgcattacca aagagataac
      781 cgcgaacatg ccatgcttaa gccgatcctt aggcgcatca acagaaagcc tgatctctac
      841 tgttcgtgat tctgtttcta ctaccggact caccatggta actcgcgcag gatattcttc
      901 cccgggccag gaaacaaaag ataccgaagc gggcagcccc gtacgtaaga ccgcaatata
      961 tttttcggga acataggtta ttatctcaag atcatcgagc gtgccaattg tcgcaacagg
     1021 ggatgaagta gatatcgtgt ctcccggatc atatggaagg gcgattatag ttcctcgaat
     1081 agtagatcta accggactaa cggcaaaggg agagcctggg cgagacggat caatccttgc
     1141 aaccacatct ccttttttca cctcagatcc aatccccgcc tcgtacgata ccagctttcc
     1201 tgcggtgtcc gcatataagg caatacggga gcgagaaaga acatctccgt taagcctgac
     1261 tcgctgcgct aaactggatg cgctgatttc aagaacagac accgtaatgg tagcagcccc
     1321 attacgggga gccttaccct gcggtccgcc ggctttttgc gggtcctttg ctttaagaaa
     1381 aagggaaaga acaagtgcta acgtcgatat accgattaac gcaagcaaaa tacttttcgt
     1441 gattcgttca aagtccttca tgtgcttcct ccatgccgca gagttctgat tcatcaagtc
     1501 caagcgcggc agacaattgt agcaagccgg ctttatacgc atatcccgca cgtaatacgg
     1561 caaggcgcgc gtttagcatt ttttggcggg cgctatccaa atcgttttga tccactaatc
     1621 cagattcaaa tccctgaacc gaaagattcc aggctctaga ggcaattcgt tcgttttccc
     1681 gggtaatttc catactgcga tataactggt ccagcagatc catttgagac agcgcctcgt
     1741 ttcgtgcaga aacaagggtt tgttcaaaag ctatggttgc gctttccact gccgcttcct
     1801 tctcccttgc gtttatccgt tccttggaac cgggtatata cccgttaagg gggatagaaa
     1861 cagagagagt aaaagtgcct gtttcgggca tattcagttc atcttgcaat cctgaggcct
     1921 gaaaattctc ggaaagcgat accgtcggac catagcgatt taaggtcgca ccagttcgcg
     1981 cgctttgcgc aagggtaata ttcagcaagg ctaggcgaac atcactgcgc cgagccaaaa
     2041 aatcgtccaa tgtgcaggaa attgttaccg gtctaaaatt gtgatccagg gtatctacca
     2101 atgtaatatc ggtgtccgga ggaagcgcga tgagcgcgca aaaattgcga acagcttgcc
     2161 gataggagcg gcgctggtcc atcaacttgg gctccaggct atccgcagca tattttgcct
     2221 gaagatacga aagctcagaa accaggccgt tatcatattt cttttttacg tataccgcct
     2281 gagaccctgc cagctgaaaa tccttctcgg ccagagccag cccttcccga tcggcaagca
     2341 agttataata cgcggtgtgt acagataaaa gaacatccct ctccaaggca tcatacgaaa
     2401 gctcggaaat ctcccgggcc aatgccgtct gccggagaga ttcccgtact gcaggctgaa
     2461 gctgcaaaga aagccccgca gaagctccta tctcggtgcc cgtgggtact gtcgtagaac
     2521 caccaagtga gtgcgtgttt ctcagagacc ccgacagggc tatcgcgggt aaaaatgcat
     2581 tccatgcgtt tttttccttt aacaccgcaa tgtcgcgctc caagcgcatc ttttttaggg
     2641 aagcattacc aagacgagca agttctatcg cctgggcaat tgaaaggtct cgagtatccg
     2701 catgcacacc aagggcaaca cagagtccag taaggaccag aaagatttta ctttttaaga
     2761 caatactcat gttcatcagt tctccaatag tacacctaca aacccaccaa taccaagtcg
     2821 aggatacaca tagttcaccc tgggaccgtt ttttttacta tatttcactt tatcctttat
     2881 tttaatcgat tagctggtat acttgaaaca agcggacggg tgtccgcacg tgatccccag
     2941 ggtccggaca ggggctcttg acctaccgga tatcgttttg ctatactttc cctacctcgg
     3001 gggtgtagct cagttggcta gagcgcttgc atggcatgca agaggtcagg ggttcaattc
     3061 ccctcacctc cacgagtttc ccaccgcgcc cttttcccca tttaatctga aaaagcaagg
     3121 cactcatgga caacccaggc atcgatcctg aactcgccgc acttcttgac ga
//
LOCUS       DAOQXQ010000127         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32540_length_3158_cov_4.113761, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000127 DAOQXQ010000000
VERSION     DAOQXQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32540_length_3158_cov_4.113761"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKH81_02895"
     CDS             complement(<1..340)
                     /locus_tag="PKH81_02895"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNQ97014.1"
                     /translation="MNNKIEKALRDLSEGKMIIITDDESRENEGDLVMDAAMVNDQSI
                     AFMVREGKGLVCVPITRDTAARLLLHQMVDTNQDRHGTAFTVSVDASDCTTGISAAER
                     AHTITVLARPD"
     gene            complement(333..986)
                     /locus_tag="PKH81_02900"
     CDS             complement(333..986)
                     /locus_tag="PKH81_02900"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNQ97015.1"
                     /translation="MFTGLIEAQGSVSSLIRQREHTAMTIYAPTIAHNLANGDSVAVN
                     GVCLTVIKTNRTHFSADISRETLCRTTFTSLMAGSPVNLERAMRADDRFNGHFVSGHV
                     DAVASVLRIETQGNFQVITFTIPTSLSPLICEKGSVAIDGISLTVSKCYDTCFSISLI
                     PHTLKHTTLHHATCGLLVNLECDILARYLAPKEDRATINNRYIRLLATSGFMGRSYE"
     gene            complement(971..2140)
                     /gene="ribD"
                     /locus_tag="PKH81_02905"
     CDS             complement(971..2140)
                     /gene="ribD"
                     /locus_tag="PKH81_02905"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNQ97016.1"
                     /translation="MSTPETHQHELWMRKALSLAVQGEGTVHPNPLVGAIIVRGNTVL
                     GQGWHHRAGEPHAEILALQNATDMGYNDFDNATLYVTLEPCCHTGRTGPCTKAIIQAG
                     IGKLVCAMKDPNPLVAGKGLQELQDAGLYIITGILEQEAKVLNRVFIHYITRSLPWVT
                     LKTAMSLDGKIATATGNSRWISGEEARSEVHHIRKNQAAILCGIGTALTDDPLLTVRL
                     KIQDTRELPAPLRVICDTHLRLPPTSQLAQTARQAPVVIAVGAHALLDSTSGIQERVN
                     ALEDLGVEILSVEEKNGKICLKGLFTILASRGVDRLLIEGGSEIAAAALQEGLVNTAI
                     FYIAPIILGGATAKGPVAGAGASTIDRGIRLEKISTRTIGKDIVIEGDIVPCLPD"
     regulatory      complement(2240..2368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2412..>3158)
                     /locus_tag="PKH81_02910"
     CDS             complement(2412..>3158)
                     /locus_tag="PKH81_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ97017.1"
                     /translation="IEGSFISAPIHTDKRVIGVVTLSRKGGNGEGFAMESRYTLEIAA
                     AFVASEINNRMLYDELEQSYFSTVSSLTRALEAKDHYTRGHSERVMQYAVGTAHALRL
                     SPEAVRRIRYAAILHDIGKIGISDSIITKTSSLTKGEFDEIKRHTEIGYDIVNENSFF
                     TEIRDLIKYHHEKMDGTGYYAKHSGDYPWEAMIISVADIYDALTSDRPYRTAYTAEQA
                     VAYLEKLVGVNFDRRILDAFQTCLKESAIA"
BASE COUNT          756 a          730 c          771 g          901 t
ORIGIN      
        1 agtctggtcg ggcaagaaca gtgatcgtgt gcgcgcgttc tgccgcagat attccggttg
       61 tacaatcaga ggcatcaact gatactgtaa aagcagttcc atggcggtcc tgatttgtat
      121 cgaccatctg gtgcaggaga agtcttgcgg ctgtgtctct tgttattggt acacaaacaa
      181 ggcctttacc ttctcgaacc ataaaggcaa ttgactgatc attaaccatc gccgcgtcca
      241 ttacaaggtc tccttcgttc tctcgcgatt catcgtcggt tatgatgatc atctttcctt
      301 ctgaaagatc cctaagggct ttttcaatct tgttattcat agcttctccc catgaatccg
      361 cttgtagcaa ggaggcggat gtatcggttg tttattgttg cccgatcttc ttttggcgca
      421 agatagcggg ccaggatatc gcattccagg tttaccaaaa ggccacaagt tgcatgatgc
      481 aaggttgtat gcttaagggt gtgggggatc agagagattg aaaaacacgt gtcataacat
      541 ttactaacag taaggctaat tccatctatt gcgactgaac ctttttcgca gatcagtggt
      601 gaaaggcttg tgggaatcgt aaaggtaata acctgaaagt tgccttgcgt ttctatgcgc
      661 aatacagaag caacggcatc aacgtgaccg ctaacaaagt gaccgttgaa tcgatcgtcg
      721 gcacgcattg cgcgctccag attaacaggg cttcccgcca taagagacgt aaaggttgtt
      781 cgacaaaggg tttcccttga gatatctgcg gaaaaatgtg tgcggtttgt tttgattacc
      841 gttagacaaa ctccatttac cgcaacacta tcgccattag caagattgtg tgcaattgtt
      901 ggtgcataaa tcgtcattgc tgtatgctct ctctgtctga taagcgagga caccgatcct
      961 tgtgcttcaa tcaatccggt aaacatggca cgatatctcc ttctataacg atgtctttcc
     1021 ctattgttcg tgtgcttatt ttttcaagtc gaatgcccct gtcgattgta gaggcgcccg
     1081 ctcctgctac cgggccttta gcggtggctc cacccaagat gattggggcg atgtagaata
     1141 tggcagtatt aacaaggcct tcttgcaggg ccgccgcagc aatctcgctc cctccttcta
     1201 ttagcaagcg gtccacacca cgggaagcca ggatggtgaa taagccttta aggcatattt
     1261 ttccgttctt ttcttccacg ctcaagattt ctacacctag gtcttcaagt gcgtttactc
     1321 tctcttgtat gcctgaggtt gaatcgagca gggcatgggc acccactgcg ataacgacag
     1381 gagcttgtcg agctgtttga gcgagttggg aagttggtgg taggcggagg tgtgtgtcgc
     1441 agattacccg aaggggtgct ggcaattcac gcgtgtcttg tatctttaat cgaacagtaa
     1501 gcagtggatc gtctgtaagg gcggtcccaa ttccacagag aatagctgcc tgatttttcc
     1561 gaatgtgatg gacttcggat ctggcttctt ctccgcttat ccagcgtgag tttcctgttg
     1621 ccgttgcaat tttcccatca agactcattg cggtttttag ggtaacccat ggcagggagc
     1681 gagtaatgta atggatgaac acccgattga gaactttggc ttcttgctca agaatcccgg
     1741 taataatgta aagccctgca tcctggagtt cttgcagacc cttgccagca acaagagggt
     1801 tagggtcctt catggcgcat acaagctttc cgattccggc ctgaatgatc gctttggtac
     1861 acggacctgt tcttcctgta tgacaacaag gctcaagtgt tacatataag gttgcattat
     1921 caaaatcatt gtagcccata tctgtcgcgt tttgtaaagc cagaatttct gcatgtggtt
     1981 caccggctct atgatgccaa ccttgcccaa gaacggtatt cccgcggaca ataattgcgc
     2041 caaccaaggg gtttgggtga acggttcctt ctccttgtac ggcaagcgaa agagccttgc
     2101 gcatccagag ttcgtgttga tgtgtttctg gtgtggacat aaaaaacccc gaaaactaca
     2161 ggtaagtttt cagggcgtaa taaacgggac aggagagttt gtacgataca atacacccgt
     2221 actaaacccg gttgtggccg tgcctcttct ttcatccaga ctttactgtc ggcttcggag
     2281 tttcaccgaa tctgctgtgc gttttaaaaa cgccctgctc gcgggctgta ccgccggtgg
     2341 ggaattacac cccgccctga agagtagctt tcggatacta tcctatggcc ggaggataat
     2401 tgtgtcaagt attatgcgat tgcggattct ttaagacagg tttggaatgc atcaagaatt
     2461 cgacgatcaa agttaacgcc aacgagcttt tccaaatacg ccacggcctg ctctgcggta
     2521 tacgcggtac gatatggccg atctgatgtt aaggcgtcat aaatgtcggc tacactgata
     2581 atcattgctt cccagggata gtctccgctg tgcttggcat aatatccggt cccgtccatt
     2641 ttttcgtgat gatacttgat caaatcccga atctcggtaa agaagctatt ttcgttaacg
     2701 atgtcatacc cgatttcggt atgtcttttt atctcgtcga attcaccctt ggtcaagctg
     2761 gatgtttttg tgatgataga atcactaata ccaattttac cgatgtcatg aaggatggcg
     2821 gcatatcgta ttcgacggac tgcctcggga gaaagtctaa gggcgtgagc agttccaact
     2881 gcatattgca taactctttc tgaatggccg cgagtatagt gatcttttgc ttccagggca
     2941 cgggtaagag aagatactgt agaaaaataa ctttgctcga gttcatcata gagcatgcgg
     3001 ttgttaatct cgcttgccac aaatgctgcg gcaatttcaa gtgtatatct gctttccata
     3061 gcaaagcctt cgccattgcc acctttgcgg gagagggtta caaccccaat aacccgtttg
     3121 tctgtgtgaa tgggagccga gatgaaagag ccttcgat
//
LOCUS       DAOQXQ010000128         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32773_length_3143_cov_3.395402, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000128 DAOQXQ010000000
VERSION     DAOQXQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32773_length_3143_cov_3.395402"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PKH81_02915"
     CDS             <1..1108
                     /locus_tag="PKH81_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ97018.1"
                     /translation="NVAEWILELDRGEGIPWKGNYSGWLQQKNDRLAREEKGESDRRK
                     QLERELEWIGMSPKGRHAKSKARINQYEKLLSEDGREKIKDTKISIPAGPRLGGLVVE
                     AKDLGMAYGDHLLFDKVNFLIPPGAIVGIIGPNGAGKTTLFNLIAGAAGQKVPVPQFD
                     AEGNILADLKEEIVTPTSGSLRVGDSVKLTYVDQMRGKLEADKTVWEQLSGGLDIIKL
                     GAVDANGRPVNGPVREVNSRAYCSWFNFSGADQSRKVGVLSGGERNRLNLAMMVKEGA
                     NVLLLDEPTNDLDVNTMRALEEALDSFAGCVLVISHDRWFLDRVCTHLIAFEADGEVL
                     WYDGNWSEYAQWRKEKYGKDADTPHRGIYRKLER"
     gene            complement(1109..3079)
                     /locus_tag="PKH81_02920"
     CDS             complement(1109..3079)
                     /locus_tag="PKH81_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ97019.1"
                     /translation="MKQDIRIGLQIDETDSPYSRYLVRGVSSFCSEKQINLIVFSGKA
                     PGWPYGFGYQGCAIHSHISKKNIDIHIIASGTQENYISREAFISHVETLTRVPLISIA
                     TRIQGIPAVMADEMKGFRDLIRHIIEVHKCKRFAVITGPETNQDSICRWETLQKVLEE
                     YDLVSSTIKAGATDYTIQAGKTVTMNLFADGICDRDALVCFSDLIAVGALEYLASAGI
                     RVPEDIVVVGYDNLDRGRFNEPPLSTVSQNVIMQGYTAASLAFDSACGKDIPLETFVE
                     TQALLRQSCGCIAADDYSIYARDIRGNEVEPPSGSVVDTGYERFYLEDDLRRLQRYLS
                     RQHAGQTFEEHSANIHESLRAFDIKSCAICLYPDKIHCADQSSFTMPSRARVVVAFDE
                     DTPKGKSVRDILFDPLERMLPVGIFSSRPRTLVVSALYHLEEQLGYIVYEPGRVHEAI
                     YETLCVQLSTMISSALIFSEKEEAGLILNSALKELESSNRVLDYVSRTDDLTGLYNRR
                     GFLSYGEKALEIALSSGKNAVIFFGDLDGLKIINDHYGHDAGDRALQAMAAILKKVFR
                     SHDIIARLAGDEFAIVALDAETSAAIALHERLSNFLTQWNEETAEPFSLSISLGYAPA
                     TRECSSLEDLLARADTVQYQEKKKKKGSSSLR"
BASE COUNT          900 a          733 c          704 g          806 t
ORIGIN      
        1 taacgttgcc gaatggattc ttgaactgga tagaggcgaa ggcatccctt ggaaaggcaa
       61 ttattccggc tggttacagc aaaagaacga tcggcttgcc cgcgaggaaa aaggggaatc
      121 agacaggcgc aaacagcttg aacgtgaact tgaatggata ggcatgagcc caaaaggacg
      181 acacgccaaa agcaaagcac gtatcaatca gtacgaaaaa cttctttctg aggatggccg
      241 agaaaaaatc aaggatacaa aaatttctat ccctgcaggg cctcgccttg gcgggcttgt
      301 tgttgaggca aaggatttgg gcatggccta tggggatcat ttactctttg acaaggtcaa
      361 cttccttata cccccaggag caatcgttgg cattattggg cccaatggtg ctggtaagac
      421 tacccttttt aatctaattg ccggtgcagc aggccaaaag gttcccgttc ctcaattcga
      481 cgcagaagga aacatcctgg ccgaccttaa agaagaaatt gttaccccaa caagcggctc
      541 gctcagggtt ggagattcag taaagctaac ctatgttgac caaatgcgag gaaaactgga
      601 agcggataaa actgtgtggg aacagctgtc gggcgggctt gatattatta agctaggtgc
      661 ggtagatgcc aatggaagac ccgtcaatgg accagtacga gaagtcaact caagagccta
      721 ctgttcttgg tttaattttt ctggagccga tcaaagcagg aaggttggcg ttctttctgg
      781 tggagagcga aacagactta accttgccat gatggtaaaa gagggagcca acgtacttct
      841 tcttgacgaa ccgacaaacg acttggatgt aaatactatg cgcgcgcttg aagaagcact
      901 ggacagtttc gcaggctgcg ttttagttat tagccatgat cgctggttcc ttgatcgcgt
      961 ctgcacccac cttattgcct ttgaggctga cggtgaagtg ttatggtacg atggtaactg
     1021 gagcgagtat gcacagtggc gtaaagaaaa atatggcaag gatgcagaca ctccacatcg
     1081 cggaatatac cggaaactgg agcgatagtt atcgtagcga agagctccct ttctttttct
     1141 ttttttcctg gtattgcacc gtatctgccc gagccagtaa atcttcgaga gaagagcact
     1201 ctctggtggc cggtgcatag cccaaactga tagaaaggga aaaaggctct gcagtttctt
     1261 cattccattg cgttaaaaaa tttgaaagtc gttcgtgcaa ggctatcgcc gcacttgttt
     1321 cggcatcaag ggcaacaata gcaaattcgt cccccgccaa gcgtgcaatt atgtcgtgac
     1381 ttctaaaaac ctttttaagg atagccgcca ttgcttgtaa ggcccggtct ccggcatcat
     1441 gaccatagtg atcgttgatt atcttaagcc catccaagtc gccaaaaaag attactgcat
     1501 ttttcccgga tgaaagggca atctccaagg ctttctcccc gtacgacaaa aaacctcggc
     1561 gattatacaa ccctgttaaa tcatctgttc ttgatacata atcaaggacg cgattagaag
     1621 attcaagctc cttaagagct gagtttagaa tgagccctgc ttcctccttt tcagaaaaaa
     1681 tcaaggcgga actaatcatt gtagacagct gtacacacaa agtttcataa attgcttcat
     1741 gcacccgccc cggctcatat acaatatagc caagctgctc ttcaagatga tacagggccg
     1801 agactaccag tgttcgtgga cgagatgaaa aaatgccaac cggtaacatc cgctcaagcg
     1861 gatcaaacaa aatatcccta accgattttc ccttgggtgt gtcctcatca aaggcaacta
     1921 ctacacgagc ccgtgaaggc attgtaaatg aagattgatc cgcacaatgt attttatcgg
     1981 gatacaaaca tattgcgcaa cttttaatat caaacgccct gagactctcg tgtatattgg
     2041 cggaatgttc ctcaaaagtt tgccctgcgt gctgtctgga taaataccgc tgcagtcgtc
     2101 gcaaatcatc ttcaaggtaa aatcgctcat atcctgtatc cacaacagaa cctgaaggag
     2161 gctcaacttc attgcctcga atgtctcgtg catatattga atagtcatcg gctgcgatac
     2221 acccgcaact ttgccgaagc agggcttgag tttccacaaa ggtttccaaa ggaatgtctt
     2281 ttccgcatgc tgaatcaaag gcaagggagg ccgccgtata tccttgcata ataacatttt
     2341 ggctaacggt actcaaagga ggttcgttaa aacgccctcg atccagatta tcatatccaa
     2401 ccacaacaat atcttccggc acccgaatgc ccgcagaagc aagatactca agagcgccca
     2461 ctgcgattaa atcgctaaag cacacaagag catcacgatc gcatatccca tccgcgaata
     2521 gattcatggt aacggtttta cccgcttgta tggtataatc cgtcgcgcca gctttaatcg
     2581 tacttgaaac aaggtcatat tcttccaaca ctttttgtaa ggtttcccat ctacaaatag
     2641 aatcctgatt tgtttctggc cccgtaataa ccgcaaagcg cttacactta tgaacctcga
     2701 ttatatgacg gattaaatcc ctaaagccct tcatctcatc ggccattacg gcagggatgc
     2761 cttgtattcg ggtcgcgata cttattaagg gtacgcgtgt taatgtctca acatgagaaa
     2821 taaacgcttc cctggagata taattctcct gtgtaccaga ggcaattatg tgaatatcta
     2881 tgtttttttt ggaaatatgt gaatgaattg cgcaaccctg atatccaaat ccatagggcc
     2941 agccaggggc tttccccgaa aatacaatca ggttaatctg tttttcgcta caaaaagaag
     3001 aaactccccg gactagatat cgggaataag gggagtcagt ttcgtcaatt tgaagtccaa
     3061 ttcgaatatc ttgtttcatc aaagcttccc gctcaattat tccttattta ccgcaatttt
     3121 gcacggttcg ggagcgctag atg
//
LOCUS       DAOQXQ010000129         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_32784_length_3142_cov_2.863946, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000129 DAOQXQ010000000
VERSION     DAOQXQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32784_length_3142_cov_2.863946"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1153)
                     /gene="pta"
                     /locus_tag="PKH81_02925"
     CDS             complement(149..1153)
                     /gene="pta"
                     /locus_tag="PKH81_02925"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNQ97020.1"
                     /translation="MNFIEGMKEKAKSYRNNVVLPEGTEPRTIQAARKILDDGIAGTV
                     TLLGNQAAVSGAAKAVNVSLAGLAVIDPSQSEWLNDFAATYYEKRKAKGMTMEQATID
                     MAGPLRFGAMMVAKGKADSMVAGAENTTGDVLRAGLTVIGTAPGTKTASSCFVMQMKD
                     SSWGSDGLMIFSDCAVIPTPSSEQLADIAVSAAKSCREFIGVDPVVALLSFSTKGSGG
                     KDDNVLRVQEAVRLLGERSCDFVYDGELQADAALIPSVTQKKAPGSPITGKVNTLVFP
                     DLGAGNIGYKLVQRLAGAEAYGPFLQGFAKPISDLSRGCSVDDIVITAAVTLVQAGKK
                     "
     gene            1288..2016
                     /locus_tag="PKH81_02930"
     CDS             1288..2016
                     /locus_tag="PKH81_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNQ97021.1"
                     /translation="MNSVGVIGLGRFGTFWTEVLSHGYTVYGNSRTPRDTAPCPQVSL
                     EEVCSLPVVFLCVSMRAMPQTLRSIAPLLKKGTLVVDTCSVKVEPVRWMREILAEDTE
                     ILATHPMFGPESAKEGLTGLPLMMHPVRMESARYDQWSHFFRSLGIMVVEMTPEEHDR
                     QAALSQALTHMIGRTLNRMGIEETPIGTLWYRKLLAIAKQVGRDSPELFLDMQTLNPF
                     AKDMRDCFERSWHEVCAELDESSR"
     gene            2055..2414
                     /locus_tag="PKH81_02935"
     CDS             2055..2414
                     /locus_tag="PKH81_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNQ97022.1"
                     /translation="MVDNKDNDLLDLDEEDYDTVLAPDIEFTGTIEFTEPFMIKGKVS
                     GQIMATSDLLIDEGALVKADIQANRVVIKGSVEGNVTATQIVHVFSSGRLNGDVSAPE
                     VVLDSGCFFSGICTMSR"
     gene            2422..3051
                     /locus_tag="PKH81_02940"
     CDS             2422..3051
                     /locus_tag="PKH81_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97023.1"
                     /translation="MKHILRIMAIVFSVLLSLSPLIAQNRPDALVLYRQGNYTEAKDV
                     CLAEIADDPDNIESHVVLSWSLVSLKEYKQAAEWAEKGRKLSRYDPRLIEILAEAQYY
                     QGFNEQALRLFQEYISIAPNGSRTSLVYYFMGEIYLRLAKYRHADMAFSAALQMENLN
                     APWWVRLGYAREMAKDYRYALEAYNRALDLNSNLQDAIRGRDRATSLLN"
BASE COUNT          827 a          793 c          741 g          781 t
ORIGIN      
        1 ttcaagaatc ttgattgcct cggcataccg tcgtttttgt ccggcaatca gggctcgctc
       61 aaagtttttt tcaaacaggt tcttgtccat aatgtcggtt cagagtatag catagactga
      121 accgacatcc tacgtttaat tcttttttct attttttccc ggcttgaacg agggttaccg
      181 cggcggtaat aacgatatcg tcaactgaac agccccgaga aaggtcagaa atcggcttgg
      241 caaagccctg aaggaagggg ccatacgctt ctgccccggc aagacgctgg accagtttat
      301 agccgatatt tcccgctcca aggtcgggga ataccagggt gttaacctta ccggtaatcg
      361 ggcttcccgg tgcctttttc tgggttacgg aaggaattaa ggccgcatcg gcctgaagtt
      421 cgccatcata gacaaaatcg caggatctct ctcccaacag gcgaacagct tcctgaaccc
      481 tcagaacatt atcatcttta cccccggatc ccttggtaga aaaagacaag agggcaacga
      541 cgggatctac accgataaac tcccggcagc tttttgccgc tgagactgca atatcggcaa
      601 gctgttcgga agagggagta ggaataacgg cgcagtcaga aaagatcatc aagccgtcag
      661 aaccccagga ggaatccttc atctgcataa caaaacagga agaggcagtt tttgttccgg
      721 gagccgttcc aattactgtc aaaccggcgc gcaatacatc accggtagta ttttctgcac
      781 cagcaaccat ggaatcagcc ttacctttgg caaccatcat ggcgccaaag cgaagcggtc
      841 ccgccatatc gatggttgcc tgctccatag tcattccctt agccttacgt ttttcgtaat
      901 aggtagcagc aaaatcattg agccattcgg attgtgacgg atctattacc gccagacctg
      961 caagagaaac attaacagcc ttcgcggccc cggaaacagc cgcctgattt cccaggaggg
     1021 ttaccgtgcc cgcgatccca tcatcgagta tcttacgtgc ggcctgaatc gtccttggct
     1081 ctgttccttc gggaagcact acattgttac gatagctttt agccttttcc ttcatgcctt
     1141 caataaaatt catcctgtac tccttgcgat ttatcgcgat tttttcttgg caaagcatat
     1201 cccccgttat cggtttgagc aaggggcacc tttcagccaa tgtcgctctt tattgacaga
     1261 aaaggccaat accggtaggt tcacaacatg aacagtgtag gcgttatcgg ccttggccga
     1321 tttggaacat tttggactga ggtactctct catggataca cggtgtatgg caattcccga
     1381 acgcctcgag atactgctcc ctgtccacaa gtaagccttg aagaagtatg ttctcttccc
     1441 gtggttttcc tgtgtgtgtc gatgcgggca atgccgcaga cgctccggag catagccccc
     1501 cttcttaaaa aaggtaccct tgtggtagac acctgttcag taaaagtaga accggtacgc
     1561 tggatgaggg aaatacttgc ggaggatacc gagatactcg ctacacatcc catgtttgga
     1621 cccgaatccg caaaggaagg cttaacgggt ttaccgttaa tgatgcatcc ggtacggatg
     1681 gaaagtgccc gctatgatca atggtctcat tttttccgtt ccttggggat aatggtcgtg
     1741 gaaatgactc ccgaagaaca cgacaggcaa gcagccctca gtcaagcctt aacccacatg
     1801 atcggccgaa cccttaaccg gatggggata gaagaaactc caataggaac cctgtggtac
     1861 cgcaaactcc tggccattgc caaacaggtt ggccgagact ctcccgagct tttcctggat
     1921 atgcaaaccc ttaatccctt tgccaaggat atgcgcgatt gctttgaacg ctcatggcat
     1981 gaggtctgtg cggagcttga cgaatcaagt cgttgacttg accgaaaccg cctatcacgg
     2041 ctacagtatc aaacatggtt gacaacaagg acaatgacct tctcgatctt gatgaagaag
     2101 attacgatac cgtattagcc cccgacattg aatttaccgg taccattgaa tttaccgaac
     2161 cctttatgat caagggaaag gtgagcggcc agattatggc taccagcgac cttttaattg
     2221 atgagggagc cttggttaag gccgatatac aggctaatcg cgttgtcatt aaaggttctg
     2281 ttgaaggaaa cgtgacagca acccaaatcg ttcatgtatt cagctcggga cgattgaacg
     2341 gagatgtgag cgctcctgaa gtagtccttg acagcggatg ctttttctcc ggtatctgca
     2401 ccatgagtag ataggcgaac tatgaagcat atactgcgga tcatggctat cgtcttttct
     2461 gtcctcttat ccctaagccc gcttattgcg caaaaccgac ctgatgccct tgtactgtat
     2521 cgacagggaa actacaccga ggcaaaagac gtttgccttg cagaaatagc cgatgatcct
     2581 gataacatag aaagccatgt cgtgcttagc tggtcgctcg tatcccttaa agaatacaaa
     2641 caagcggcag aatgggcaga aaagggtcga aaactatcac gatatgaccc cagactcata
     2701 gaaatacttg cagaagccca atactatcag ggttttaatg aacaagccct tcgtcttttc
     2761 caggaataca tctcgattgc cccaaatgga agcagaacat ccctggtgta ttattttatg
     2821 ggagagattt atctccgtct tgccaaatat cgacacgccg acatggcctt ttcggcagca
     2881 ctccaaatgg aaaaccttaa cgccccctgg tgggtccgac tgggctatgc gcgggaaatg
     2941 gccaaggatt atcgatatgc cctggaagcg tataatcggg ctttggactt aaactcaaat
     3001 ctgcaggacg cgatcagggg cagagaccgg gcaacctcct tattgaactg atggaaaccg
     3061 ttctttttaa aacccttggg tgcaaattaa accaaataga aacagaatct ctggctcaag
     3121 cctttagtga agcaggattt cg
//
LOCUS       DAOQXQ010000130         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_33274_length_3112_cov_3.669938, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000130 DAOQXQ010000000
VERSION     DAOQXQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33274_length_3112_cov_3.669938"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /gene="aspS"
                     /locus_tag="PKH81_02945"
     CDS             complement(<1..1428)
                     /gene="aspS"
                     /locus_tag="PKH81_02945"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ97024.1"
                     /translation="MKRTVTCGALRKADEGKTVVLNGWVHRKRDHGGISFINLRDRYG
                     LTQVVVDADAEEALRTIAAELKMEYCLAVEGIVRARPDSMINPDMATGEIEVCAKQIT
                     ILSRSEVLPFVIDEKTNASEELRLKYRYLDLRSQTMQNHLALRSKVAFATRQYLTNLD
                     FLELETPTFIKSTPEGARDYLVPSRLYPGQFYALPQSPQLYKQLLMVSGFDRYFQIAR
                     CYRDEDARGDRQPEFTQIDIEMSFVSRDDVLSLIEGMMGHIFRTTMNVELPVTFRRIS
                     YDDSIDLYGTDKPELRFGMEMQDGAFLAEEGSFQAFKDALSAGGKIKALVVPGVAEKY
                     SRKLIEDLESTAKIYKARGLGWMKVGGNPDAPILEGGISKFFEGKAEQICATLGARYG
                     DLLLFVADPKAKIACTALGAVRTRLGKDLNLCAEGTFEFAWIIDFPMFEWNEDALKWD
                     TAHHMFTWPQTQYHATLESDPGSVKG"
     gene            1525..>3112
                     /locus_tag="PKH81_02950"
     CDS             1525..>3112
                     /locus_tag="PKH81_02950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNQ97025.1"
                     /translation="MTSEDLREKNERIKKLVMTLIWHLMGVLIVLGIIFPVSTYLALN
                     SIFLTEQMVTAKMAQTMVSDTIATKKRLLSFAAGTRSVRTANFSGLLQEELRGIPENE
                     LPELRGLFQHLLNSFSSFRFFSYLTYDSVRPYVIHPYQTQLELTLSDYQAGFAYREWA
                     RKTHARYEVWNGTGPIEPYVSNAFLSKPGLIPGVSVSVTVINDEKQPVGILYANIILD
                     KLSDFVKNLSFGKTGRVFLVDAEGFLLAHPTISAGREVVDEEGNKTWELRSVADHPVV
                     ANALQGRFSPGVFRLTGESKAVLATCLTVPDTGWIIVVEQETAEILNRVKLYWIVVIV
                     LVFLTIAVSAVVFIMIAREIAETTREHDELIVISETDPLTGLLNRRSMLSRMVQFVQN
                     YQETKQPFVIALFDIDDFKLVNDTHGHVFGDLVLREIAARTASVLRVDDLLFRWGGEE
                     FLVVVKNCNLLRGKGIAEKIRRVVADAPISDDRISISITVTLGVCEYQGGSIDALIIH
                     ADEALYEGKRTGKNRVVGASS"
BASE COUNT          789 a          657 c          794 g          872 t
ORIGIN      
        1 tccctttacg cttccaggat ctgattcaag ggtcgcatga tactgggtct gaggccaggt
       61 aaacatatga tgagcggtat cccattttag agcatcttca ttccattcaa acatggggaa
      121 atcgataatc caagcaaact caaaggttcc ttccgcacaa agatttagat cttttccaag
      181 ccgtgttcga acggcaccca gtgcggtaca ggcaatcttt gctttggggt ccgctacaaa
      241 gaggagcaag tcgccatagc gtgcgccaag cgttgcgcat atctgttcgg cttttccctc
      301 aaaaaacttg gaaataccac cttcaaggat gggtgcatcg ggatttcctc ctaccttcat
      361 ccagccaagg ccgcgggcct tgtagatttt ggcagtgctt tcaaggtctt cgatgagctt
      421 cctgctatat ttttctgcga caccaggaac aaccagggct ttgatttttc caccggcgct
      481 caaagcatcc ttgaatgcct ggaagcttcc ttcttccgcg aggaaggccc cgtcttgcat
      541 ctccatgcca aagcggagtt cgggtttgtc ggttccgtac aaatcgattg aatcatcgta
      601 ggaaattcgt ctaaaagtga caggaagttc cacattcatt gtagtgcgga agatatgacc
      661 catcattcct tcgataaggg agagtacgtc gtctcgagaa acaaagctca tctcgatatc
      721 aatttgagtg aactcaggct gacgatcgcc tcgtgcatcc tcgtcgcgat aacagcgtgc
      781 aatttggaaa taacggtcga accctgaaac cataagcaac tgtttataga gctggggcga
      841 ctggggaaga gcgtagaact gtccaggata taatcgggag ggaaccagat agtcgcgggc
      901 gccttccggg gttgatttga taaatgtagg agtttctagt tcaagaaaat ctaggttggt
      961 aaggtattgc cgggttgcga aagcgacctt ggatctgagc gccaggtgat tttgcatagt
     1021 ttgagagcga aggtccaggt agcgatattt aagacggagt tcttcgctcg cgttggtttt
     1081 ttcgtcgatt acaaaaggaa gcacctctga acgggaaagg atggtaatct gtttggcaca
     1141 cacctctatt tcgccggtgg ccatatcagg gtttatcatg gaatccggcc tggctcggac
     1201 aatgccttct acagcaaggc aatactccat ttttagctcc gcagcgattg tcctgagggc
     1261 ttcttcagcg tcggcatcga ctacaacctg ggtaagacca tagcggtccc gaagattgat
     1321 aaaagagatt ccaccgtgat cgcgtttgcg atgcacccat ccgtttaata caactgtttt
     1381 tccctcatca gccttgcgca aggcgccgca ggtgacggtc cgtttcatga tttccatggg
     1441 agtgagtata tcgggaagga tgattcaatg acaaggagct aactattttg catccttggc
     1501 aggagataca ctatactcaa gggcatgacc agcgaagacc tacgagaaaa aaatgaacga
     1561 ataaaaaaat tggtgatgac tcttatttgg catttaatgg gagttcttat cgttttggga
     1621 ataatctttc cagtttctac ctatttggcc cttaactcca ttttccttac ggaacagatg
     1681 gtaacggcca agatggccca gactatggta agtgatacaa tagccaccaa gaaacgcttg
     1741 ctttcttttg ccgctggaac tcggtcggtc aggacagcca attttagcgg tttattacaa
     1801 gaagagttgc ggggcatacc ggaaaatgag cttccggaac taagaggttt gtttcagcat
     1861 ctccttaaca gtttttcgtc ttttcggttt ttttcttatt taacctacga ttctgtacgt
     1921 ccgtatgtaa ttcatccata ccaaacccag cttgagctta ctctttctga ttatcaggcc
     1981 ggatttgcct atagagagtg ggcgcgaaaa acgcatgccc gatatgaggt atggaatggc
     2041 accggtccta ttgaacccta tgtttctaac gcctttttgt ctaagccggg acttattcca
     2101 ggggtttctg tgtctgttac ggtaatcaat gatgagaaac agccagtcgg gattttgtat
     2161 gcaaatatta ttcttgataa attatcggat tttgtaaaaa atctgtcgtt tggaaaaacc
     2221 ggcagggtct ttttggtaga tgccgagggt tttttgctgg ctcatccgac tatttcggca
     2281 ggacgagagg ttgttgatga agaggggaat aaaacctggg aattacggag cgttgcagac
     2341 catcctgtgg tagcaaatgc cctgcaggga cgatttagtc cgggtgtatt tagacttacc
     2401 ggggaaagca aagcagtgtt agcgacctgc cttaccgtgc cagatactgg ctggattatt
     2461 gtggttgaac aagaaacggc agaaatactt aatcgcgtaa aattgtattg gattgtggta
     2521 attgtccttg tctttttgac tattgctgtg tcggccgttg tctttattat gatagctcga
     2581 gaaatcgcgg aaacgacgcg ggaacatgac gagcttatcg ttatctccga gacagaccct
     2641 cttacaggac ttttaaaccg aagaagtatg cttagccgga tggttcagtt tgttcagaat
     2701 tatcaggaaa ctaagcaacc ctttgtaatt gccttgtttg atattgatga ttttaaacta
     2761 gtaaacgata cgcatgggca tgtgtttgga gatcttgttt tacgggaaat tgccgcgcgt
     2821 actgcttcgg tgctacgagt cgatgattta ttgttccgct ggggtggaga ggagttcctg
     2881 gtggtggtaa aaaattgcaa tttattgcgg ggaaaaggta tcgccgaaaa gattcggcgt
     2941 gtagtggcag atgctcctat tagtgacgac cggattagta tatccattac ggttactctt
     3001 ggtgtgtgcg aataccaggg tggttcaata gatgcgctga ttattcatgc agacgaggcc
     3061 ttgtatgagg gtaaacgaac agggaagaac cgcgtcgtgg gagcaagctc gg
//
LOCUS       DAOQXQ010000131         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_33313_length_3110_cov_3.171522, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000131 DAOQXQ010000000
VERSION     DAOQXQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33313_length_3110_cov_3.171522"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PKH81_02955"
     CDS             complement(<1..1677)
                     /locus_tag="PKH81_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNQ97026.1"
                     /translation="MSFNQLPGRKGLYNSEFEHDSCGVGFVCDIKGRISRKIVEDGLE
                     MISRMNHRGASGADPDSGDGAGILVQLPHRFLTKVASRHGISLGEIGSYGCGIVFLPK
                     SRSLRERCRKDMEEACNALSLKIALWRPVPVREDLIGMSARQSMPCMEMVFVVPATEG
                     PFSTLPLEQRLFLARKKAEWRIIERDGISPSDFYVSSLSSRTIVYKGMLTPPQLAPFF
                     PDVQDPDFESAIALIHSRFSTNTFPSWDRSQPFRYLAHNGEINTLKGNSTWMRSRESA
                     LAKPEWGDDLSELFPLIQKQGSDSQALDNVFEFLLQSGRDMSHVMSMLVPAAWEGDDS
                     IDPSVRDFYRYHRCMMESWDGPAALAYTDGTIVAASLDRNGLRPGRYLVTSDDRVIMA
                     SELGVLDVPPEMIVQSLRLEPGKMLYIDTSRALIISDREVKLSLARRAPWGQWLSQSI
                     STLEQKPVGSSVSCLEDTVFRSLYWTREETEMIVAPMAINGLEPIGSMGNDAPHAVLS
                     EKTVPLFSFFRQLFAQVTNPPIDPLREGCVMSLETVIGPQGNILDQGPSLA"
     gene            complement(1869..2924)
                     /locus_tag="PKH81_02960"
     CDS             complement(1869..2924)
                     /locus_tag="PKH81_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ97027.1"
                     /translation="MAISPHLLGRINEKSGQPKFLQIADRVRELIDSGALSLGDRLPS
                     VNKVIAHFSVSRDTVVKAYQELKDRGIIESTPNKACFVSNVLLHEGAKKVLFLSDSMA
                     PYKEQLYFGLIDSLTSGFYVDIVTHGDNFEILKGVYEKYKAMRDCSALLVIPTAAQNR
                     EEDYFKFVNPGNLLFLDRRLPSLRHPAVWQDFTDGFFHALSPHADLLKRYRRLVFLTK
                     FYTNPIIEEMKEGLALFCGVEGIPFVHEHTLFTDRDIKGKLCPHKKDLYVVLDDHLLV
                     SLMDACAQNSLVIGPDVGIIAINDGPFYSRLPVPISVLTTNFYAMGEAAAHFVINGCV
                     PSAPIPTVLTVRASLLD"
BASE COUNT          926 a          758 c          715 g          711 t
ORIGIN      
        1 ggcaagggaa ggcccttggt caagaatatt tccctgtggg ccgatgaccg tttcaagact
       61 catgacacaa ccctcgcgca gaggatcaat cggaggatta gtaacctgtg caaaaagctg
      121 tctgaaaaaa gaaaataagg gaaccgtttt ctcggatagc accgcatgcg gcgcgtcatt
      181 gcccatggac ccaatcggtt caagaccatt aattgccatg ggagcgacga tcatctctgt
      241 ttcttcgcga gtccaataca gagatcggaa cacagtatct tccagacacg aaacagaaga
      301 gccaacaggc ttttgctcca gagtcgatat agactgcgac agccattgac cccatggcgc
      361 tcggcgagca agcgaaagct taacctcacg atcgctaatg ataagcgcgc gggatgtatc
      421 aatatacaac atcttccctg gctcaagacg gagcgattga acgatcatct cgggaggtac
      481 atcaagaacc ccaagctcgg aggccatgat gacccgatcg tcggaggtta ccagataccg
      541 tcccggacgc aaaccattac gatcaagaga agctgcaaca attgttccat ctgtataggc
      601 aagggctgca ggaccatccc agctttccat catacaacga tgatagcgat aaaaatcgcg
      661 aaccgaagga tcgatagaat catccccctc ccatgcggcg ggaacgagca tactcataac
      721 atgactcatg tctctacccg actgcaggag aaactcaaac acgttatcca aagcttgtga
      781 atctgaaccc tgcttctgga tcaaggggaa cagctcggaa aggtcgtcgc cccattctgg
      841 ttttgcaaga gcggattccc gcgagcgcat ccaggtagaa ttgcccttta gggtatttat
      901 ctctccatta tgcgcgagat accggaatgg ctgagagcga tcccacgaag gaaaggtatt
      961 ggtggaaaaa cgagagtgaa taagagcaat agcggactcg aagtctggat cctgtacatc
     1021 gggaaaaaag ggtgcgagct gaggcggtgt taacatccct ttatacacaa tcgttcttga
     1081 agacaaggat gaaacataaa agtccgaggg actgataccg tctcgctcga taattctcca
     1141 ttcagccttt ttgcgcgcca gaaacagacg ctgttcaaga ggaagggtcg aaaaaggtcc
     1201 ctctgtggcc ggaacaacaa agaccatctc catacagggc atggattggc gagcagacat
     1261 accgatcaga tcctcacgaa caggaacagg tcgccagagg gcaatcttga gggacagagc
     1321 attacatgct tcttccatat ccttacgaca tcgttcccgc aagcttcgtg attttggtaa
     1381 aaacacgata ccgcatccat aagaaccgat ttcaccaaga gaaattccat ggcgagatgc
     1441 gacctttgta aggaagcggt gtggtaattg gaccaggatt cccgcaccgt caccggagtc
     1501 gggatccgca cctgaagcac cacggtgatt catacgggat atcatctcaa ggccgtcttc
     1561 cacaatcttg cgggaaatac gtccctttat gtcgcaaacg aatccaacgc cgcagctgtc
     1621 atgctcaaac tcgctgttat acaggccctt acggccgggt agttgattaa acgacactcg
     1681 catactcctc tgtatggacc taaaggacca ttcatagaat tactgcaaga ggcgtgccaa
     1741 caccggtcga tacgcctaaa tccctaagtc accgaggttt ataggtagaa gggtgatata
     1801 acagacagcg gatggtgtat aaaaaatatg cataatagag gtttatgcat actaaagagg
     1861 cgttctgatc aatctaatag tgaagcgcga acggtcaata ccgtcggaat tggagcagac
     1921 ggcacgcatc cgttaatcac aaaatgagcg gcggcttctc ccatcgcata aaaattggta
     1981 gtcaagacac ttatcgggac gggtaaacgg ctataaaacg gcccatcatt tatagcgata
     2041 atccctacat caggaccaat aaccaatgaa ttttgcgcac aggcatccat gagagatacc
     2101 agcaagtgat catccagaac aacatacagg tcttttttat ggggacagag cttgccctta
     2161 atgtctcgat cagtaaacaa cgtatgttca tggacaaagg gaatgccttc tacaccacaa
     2221 aaaagggcta aaccttcctt catttcctca ataatgggat ttgtatagaa ttttgttaaa
     2281 aaaacgagtc gacgatagcg cttgaggaga tcggcatgtg gggacagagc atgaaaaaaa
     2341 ccatcggtaa aatcttgcca caccgcagga tgccgtaaag aaggaagccg tcgatcaaga
     2401 aaaagcagat ttcccggatt cacgaactta aaataatctt cttccctgtt ttgtgccgct
     2461 gttggtataa ccaggagagc agaacagtcc cgcatagcct tgtatttttc gtatacaccc
     2521 ttaaggattt caaaattatc tccgtgggtg acgatatcaa cgtaaaatcc agatgttagt
     2581 gaatcaataa gaccaaaata gagctgttcc ttataaggag ccattgaatc tgataaaaaa
     2641 agaaccttct tggcaccctc atgaagcaga acattgctta caaaacaggc cttattcggg
     2701 gtggattcaa taataccccg atcttttagc tcttgatatg ctttaacgac ggtatccctg
     2761 gatacggaaa aatgagcgat aaccttgttc acggacggta atctatcccc gagggacaga
     2821 gcaccagagt caatcaactc ccgaacccgg tcggcaatct gtaaaaactt gggttgacct
     2881 gatttttcgt ttattcttcc caacaagtga ggggatatcg ccatacatgc tccctttttt
     2941 tcttcgtcca tactagcaca catacagacg attttactag cagaacggaa cagaatggta
     3001 tacaataccc gcaggagcca gctagatgca tacatcattc ttgattcgtc ttaccgtacc
     3061 ccttttcgcc cttgtgcttg tttccgcctc tttcgccaat gacggggata
//
LOCUS       DAOQXQ010000132         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_33379_length_3105_cov_3.481967, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000132 DAOQXQ010000000
VERSION     DAOQXQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33379_length_3105_cov_3.481967"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PKH81_02965"
     CDS             <1..1117
                     /locus_tag="PKH81_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ97028.1"
                     /translation="KELWSGNDSGKPIEIYIPENEADEADFVSEMIQTIRMNEKRSYA
                     DFGVLIRANTLSRAVEEAFLEANIPYLMSGGTSFFQRKEIKDIISYLRVIANPDDDIN
                     LLRIINTPRRGIGRKTIEDVSVIATGRKCSLWESIQALLSEEGSPLSEKSKADLETFS
                     LFIQEHRTTMLSGKDLSAKVKKMVDEIDYWSYLVQEYQKNEKAARYKFLNIDSLITSI
                     STWEKDPDNMKTSLYDYLNRITLITRDDMEEDEDSGKVNLMTIHASKGLEFPVVFIVG
                     AEDGLIPHARCVEETGGDVEEERRLFYVAITRARDKLFITSCRRRRKMGGVVECTPSP
                     FLEEIPASLVEYHEPSQPVETDEAIDILAKMKARFTM"
     gene            1141..1638
                     /locus_tag="PKH81_02970"
     CDS             1141..1638
                     /locus_tag="PKH81_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97029.1"
                     /translation="MNREIKDIDLDSVGARALASVYNRERELRAMSELTPIPAFRQPG
                     EAITFPPVDASAATWGILWVDASSFGFFIHEKETQLSRLRQSGSNTEKKTLSIEIPKN
                     SIAGIHITTKKTKSRINQFLDRFSGKSDTTIEVAWNTGEGLVSLRFSVYERDAHFIET
                     LEMLH"
     gene            complement(1650..2258)
                     /locus_tag="PKH81_02975"
     CDS             complement(1650..2258)
                     /locus_tag="PKH81_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97030.1"
                     /translation="MRTRCACPRYVILVAIVCGIILAFAVAFLLGYIPAVHQLSDLSR
                     LGVSDGFVPVSLTIFGRSVDTISARLAFYSQSQDLLGTVERSWAGWELRLECIMVSVP
                     SGWLVFPFRLSSDESAAFGGVSLMRYYSRSSFPSLYDRSALTDTEERALSHVFSLVQT
                     ERWIPPIFGTLHRQAVSIRSFEPGKEYRLFVTRDGTLHLLEN"
     gene            complement(2255..>3105)
                     /locus_tag="PKH81_02980"
     CDS             complement(2255..>3105)
                     /locus_tag="PKH81_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNQ97031.1"
                     /translation="IEGLVENPPDTSAIEIPHICTSCSTALVNEGTRLYCPNPRCPKV
                     IHHRISKWLTVLDVRDFGDALIQQLFASSRVRSISDLYTLTVEELASLERMGEKSASK
                     VLKALDSRRDLRLSQFIAGFDIDGIGETMVDKLIAAGFTSLESLLSAKETDFAQVYQF
                     GDILARSLRAGLDELGPQMHSLVASNTIRIQEVEGSGGLSGVSFCFTGELTTLTRSRA
                     EQLVKDSGGSVKSAPVKGLSYLVTNDPQSSSSKTRKARELGIPFIDEEDFLKMLGREA
                     GVEEVR"
BASE COUNT          939 a          713 c          736 g          717 t
ORIGIN      
        1 aaaagagtta tggtccggca acgactcagg aaaacctatc gagatttata ttcccgaaaa
       61 tgaggcagac gaagcagatt ttgtgtcgga aatgattcaa acaatcagaa tgaacgaaaa
      121 acgaagctat gcggattttg gagtactgat tcgggcaaac accctctcca gggcagttga
      181 agaagccttt cttgaagcga acattcccta tcttatgtcc ggtggcacga gttttttcca
      241 gagaaaggaa attaaagata ttatcagtta cctgagagta attgcgaatc cggatgatga
      301 catcaatctg cttagaatta tcaatacacc caggcggggc attggcagaa aaacgattga
      361 ggatgtatcg gtaatagcca caggacgaaa gtgttcgctg tgggaatcaa tacaggcact
      421 tctgagcgag gaaggcagcc ctctttccga aaaaagcaag gcagaccttg aaaccttttc
      481 gctctttatt caggaacacc gaaccaccat gctctctgga aaagatcttt ctgcaaaggt
      541 aaaaaaaatg gtcgatgaaa tcgactactg gagttatctg gttcaagagt atcaaaaaaa
      601 tgagaaagcg gcacgataca agtttcttaa tatcgatagc ctcattacgt ctatctctac
      661 ctgggaaaaa gaccctgata acatgaagac ttctctgtat gactacctta atcgcattac
      721 cctgataacc cgtgacgaca tggaagaaga tgaagattct ggaaaagtaa acctgatgac
      781 tattcatgcc tcaaagggtc ttgagtttcc cgtggtgttt atcgttgggg cagaagatgg
      841 cctaattccc catgctcgct gtgtggaaga aacgggagga gacgtagaag aggagcggcg
      901 actgttttac gttgcgatta cccgtgcccg agacaagctt tttattacca gttgccgcag
      961 aaggcgcaaa atgggaggtg ttgttgaatg tactccttct ccttttttgg aagaaatccc
     1021 cgcatccctg gttgaatacc atgagccgag tcagccagta gaaaccgatg aagcaattga
     1081 tatactcgct aaaatgaagg cgcgctttac catgtaaacg ctacgactat cggaggacat
     1141 atgaacagag agataaaaga tattgatctt gattcagttg gggcacgggc ccttgcttcg
     1201 gtatacaacc gggaaagaga attgcgggcg atgtctgagc taactccaat tccagcgttt
     1261 cgccaacccg gagaagcgat tacctttcct ccggtggacg catccgccgc aacctgggga
     1321 atcctttggg tggacgcttc gagctttggc ttctttatcc atgaaaagga aacacagtta
     1381 tcccgactgc gacaaagcgg ttccaatact gaaaagaaaa ccttatcaat tgaaattcca
     1441 aaaaactcta ttgcgggaat tcacataaca acaaaaaaga caaaaagccg cattaatcaa
     1501 tttctggata gattttccgg aaaaagcgat accacgattg aagtagcatg gaacaccgga
     1561 gaaggactgg tatcactgcg gttttctgtg tatgagcgag acgctcactt tattgaaacg
     1621 cttgaaatgc tacactaatc cagaacgaat cagttttcca aaagatgcaa ggttccgtcc
     1681 cgagttacaa ataacctata ttcttttcct ggttcaaaag agcggataga gaccgcctgt
     1741 cgatgcaagg taccaaagat cggcggaatc catcgttctg tctgtaccag ggaaaaaaca
     1801 tgagagaggg cacgctcttc tgtatcggtt aatgccgatc gatcgtacag tgagggaaag
     1861 gaagatcggg aataatagcg catgagggac acgcctccaa aggcagcgct ctcgtcgctc
     1921 gacaaccgga atggaaaaac gagccaccca gagggaacac tgaccataat acattcaagc
     1981 ctgagttccc aacctgccca ggaccgctcg acagtaccga gcaagtcttg cgattgactg
     2041 taaaaagcaa gtcgtgcgct aatagtatcc acactccggc caaagatagt taggcttacc
     2101 ggaacaaatc catctgacac acctagccgg gaaaggtcag aaagctgatg cacagcggga
     2161 atatacccaa gcaaaaaggc aactgcaaaa gccaggatta tgccgcacac aatcgcgaca
     2221 aggattacat atcgggggca cgcacatcgc gtcctcaacg aacttcctcc acacctgctt
     2281 cccgtcccaa catcttgaga aagtcctctt catctataaa gggaatgccc aattcacgag
     2341 cctttcgagt ctttgaagaa gacgattgcg gatcgttagt aaccagatat gacagaccct
     2401 taaccggggc ggatttgaca gaaccacctg agtccttgac aagttgttca gcacgacttc
     2461 tggtaagcgt agtcaactct ccggtaaagc aaaaggaaac acctgacagg ccacctgagc
     2521 cttcgacttc ttgtatgcgt attgtgttgg atgcaacaag actgtgcatc tggggtccca
     2581 gttcatcaag acctgcgcgt aaggaacggg ccaggatgtc gccaaattga tagacctgtg
     2641 cgaaatctgt ttcctttgcc gaaagaaggg attcaaggct tgtaaatcct gcagcgatta
     2701 atttatcgac catagtttcg ccaataccgt caatgtcaaa cccggcaata aactgggaaa
     2761 ggcgtaagtc ccggcgacta tcaagggcct tgagcacctt gctcgcgctt ttttctccca
     2821 ttctttccag agaggcaagt tcttccactg ttagggtata caaatctgaa atggagcgaa
     2881 cacgggagga agcaaacaat tgttgtataa gggcgtcgcc aaaatcccga acatccaaaa
     2941 cggtaagcca tttagaaatg cgatgatgaa ttaccttggg acaacgcgga ttcggacaat
     3001 acaggcgcgt accttcattt accagggcag tggaacaact tgtacagata tgaggaatct
     3061 cgatagcgct ggtgtcgggt ggattttcaa ccagtccttc tatct
//
LOCUS       DAOQXQ010000133         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_34491_length_3037_cov_2.881958, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000133 DAOQXQ010000000
VERSION     DAOQXQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34491_length_3037_cov_2.881958"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..217)
                     /locus_tag="PKH81_02985"
     tRNA            complement(144..217)
                     /locus_tag="PKH81_02985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Arg,seq:tct)
     gene            complement(221..293)
                     /locus_tag="PKH81_02990"
     tRNA            complement(221..293)
                     /locus_tag="PKH81_02990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Pro,seq:tgg)
     gene            complement(345..1058)
                     /locus_tag="PKH81_02995"
     CDS             complement(345..1058)
                     /locus_tag="PKH81_02995"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNQ97032.1"
                     /translation="MNVHNLMEELVYSGVNDLFDTAQKTKPEWLTCTCNQCRLDTICY
                     VLNRVAPRYIKSGRGLAHNQLEELQDKAQLMADINRITLEGMKQVLSTQRPHIQEQNT
                     LPQSPVFNFPTFVGRILDGLTFEPVKDIPVHLYMSNEIAQPIDSSWVNPYPITSHTPG
                     TFTFWVKPLSASHETDKKVIPFEIRVEKKGYDPLRYYFEIAVTGESVIRTAYNSNHSF
                     ILPDLYLFPSEDPHSSMQD"
     gene            complement(1088..2377)
                     /locus_tag="PKH81_03000"
     CDS             complement(1088..2377)
                     /locus_tag="PKH81_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ97033.1"
                     /translation="MTIDAIRDGIRFTEEIRDPVWKSIRLTPTLKTITRTAPFLRLYR
                     IKQLGPTEYIYPGATHSRASHSLGVYHLALRMLKVILDAGGREWLTSLGCWSFLAAAL
                     LHDLGHFPFTHSLKELPLREHESLTADAIEGDLLSSLICEAGGDPLICAAIIDQEKDI
                     PLSFEQEVLFFRKLLSGVLDPDKLDYLNRDAYYCGVPYGVQDTDFILSRITCDKTHGF
                     TLDSSSIMSVEGILFSKYLMYRSVYWHKNVRMATAMMKKTLYKALHLGILAPEDLYPL
                     DDEGIYALIASLDFPERDVALSIRRRSFYSIIGEFDFDSASPAMQALENLEQRTLSEQ
                     ICADFLSQRLHKSIHAYEVVIDIPERISFESDILVKDEEKTFSSSSTVFSGAVVQRFT
                     SSLRKIRIAVHPSLGSNTLLQNLDKQELAHCLGLGYT"
     gene            complement(2394..>3037)
                     /locus_tag="PKH81_03005"
     CDS             complement(2394..>3037)
                     /locus_tag="PKH81_03005"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758295.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNQ97034.1"
                     /translation="GMLIEPLPLERALKTQILRGPNIKECPACPELPDTLTLPLLLKT
                     ADNVSTDDIMPAGAKVLPLRSNIPEISKFTFSRLDTSFYDRALENKGCLVLGGENYGQ
                     GSSREHAALAPMYLGVRIVLVKSFARIHKANLINYGILPLVLDDQSIYDKLSQGDILT
                     VSNIHTSLTTGSSFTLERPGDGLVFTAKNDFDERSRKILLSGGLASYTRKGGQ"
BASE COUNT          837 a          637 c          729 g          834 t
ORIGIN      
        1 tagcaacgat aggccgtgta atagccataa agtagggaat taaagcagat ccagttacag
       61 gggaaggaaa ttctttggtt gttgaatcag ggaaggtgat tcggatattc ggcataggca
      121 tcctccgctg ttgatgaaga caacgggacc ggagggactc gaacccccta cctactgctt
      181 agaaggcagt tgctctatcc ggatgagcta cgatcccaaa tcgggatgag aggagtcgaa
      241 cctccgatct tctggtccca aaccagacgc cttagccact aggctacatc ccgtagattt
      301 cacattgtag ggaaactaag cctcgatgtc aaggataggc ttttctaatc ttgcatggaa
      361 gaatgtggat cttccgatgg aaaaagatac aaatctggaa gaataaacga gtgatttgag
      421 ttatatgcgg tccgtatgac agattccccg gttactgcta tttcgaagta atatcgcagc
      481 ggatcatatc cttttttttc aacacgaatc tcgaaaggaa tgaccttttt atctgtttca
      541 tgagaagcgc ttaggggttt aacccaaaac gtaaacgttc ctggtgtgtg ggatgtaatg
      601 ggatagggat taacccacga actatctatg ggttgggcta tctcattgga catatataga
      661 tgaaccggta tgtccttaac cggttcaaac gttaatccgt caagaatcct gcctacgaag
      721 gttggaaagt taaacaccgg tgattggggt agggtgttct gttcttgaat gtggggtctt
      781 tgagtggata agacctgctt catcccttca agggtaattc tattgatatc tgccattaat
      841 tgggctttat cttgtagttc ctctagctgg ttatgggcaa gtcctcgtcc tgacttaata
      901 tatcggggag ctacacgatt taacacataa cagattgtat ccaggcgaca ttgattacag
      961 gtacaggtaa gccattcagg cttggttttt tgggcggtat caaagagatc gtttacacca
     1021 ctgtatacga gttcttccat caggttatgt acattcatat gatcctccca agaccgccga
     1081 tgcttgtcta agtgtaaccc agtccaaggc aatgcgcaag ttcctgtttg tccaggtttt
     1141 gaagcaatgt gttgcttccc agtgaggggt gaacagcgat tcggattttc ctgagagagg
     1201 aggtaaatcg ttggacaaca gcaccggaga atacggtaga acttgaacta aacgttttct
     1261 cttcatcctt taccaggata tcactttcga aagaaatcct ttcaggaata tcaatgacaa
     1321 cttcgtaggc gtgtatgctt ttatgtaatc gctgtgaaag gaaatcagca catatctgtt
     1381 ccgacagtgt tctctgttca aggttttcca gtgcttgcat tgctggactg gcagaatcaa
     1441 agtcgaactc tccgataatg gagtaaaagg atctacggcg tattgataga gccacatccc
     1501 gctccggaaa gtccagagac gcaataagag catagattcc ctcatcatcc agaggataca
     1561 gatcttctgg agcaaggata cccagatgaa gtgccttata cagggttttt ttcatcatgg
     1621 cggtggccat acgtacattc ttatgccaat acacagaacg atacattagg tatttagaga
     1681 acagtatgcc ttcaacggac ataatcgatg acgaatcaag tgtaaacccg tgtgttttgt
     1741 cacaggtaat tcgtgaaaga ataaagtcgg tatcctggac gccataggga acaccacaat
     1801 aataggcatc tctgtttaga taatccaatt tatctggatc gagtaccccg gacagaagct
     1861 ttctaaaaaa aagaacctct tgctcaaagg ataagggtat gtctttttct tgatcaatta
     1921 ttgccgcaca tattaagggg tctcctcctg cttcgcatat cagggacgat aataggtctc
     1981 cttcaatcgc atcagcagta aggctttcgt gttcgcgtaa aggcaattct ttaagtgaat
     2041 gggtaaatgg aaagtgaccc aggtcatgta atagggccgc agcgagaaaa gaccagcagc
     2101 ccagcgaagt aagccattct cgccctcctg cgtcaagaat aactttgagc attcgaagcg
     2161 ccagatggta tacccctaat gaatgtgagg ctctgctatg ggtagcccca gggtaaatat
     2221 actcagttgg tccaagttgc ttgattcgat agagtcgtaa aaaaggtgcg gttcttgtta
     2281 ttgtcttaag cgttggagtt aaccggatgc ttttccacac agggtcacga atttcttcgg
     2341 taaagcggat gccgtccctg atcgcgtcaa tagtcatccg gtatgtctcc ttgttactgt
     2401 ccacccttac gggtatacga tgcaagtccc cccgaaagaa gaatcttgcg ggaacgctcg
     2461 tcaaaatcgt ttttagccgt aaaaactaat ccatcaccag ggcgttccaa tgtaaatgaa
     2521 cttccggtgg taagagaggt atggatgttt gataccgtaa ggatgtctcc ctgagacagt
     2581 ttgtcatata tagattgatc gtcaaggaca aggggaagaa tgccatagtt tatcaaatta
     2641 gccttgtgaa ttcgtgcaaa ggattttacc aatacgatac gaaccccaag atacattggg
     2701 gcaagtgccg catgttctcg tgatgatcct tgtccataat tctctccacc aagtaccagg
     2761 caacctttgt tctccaaagc tctatcataa aagctggtat cgaggcgcga gaaggtaaac
     2821 tttgaaatct ctggaatatt tgacctgagc ggtaagacct ttgctcctgc tggcatgata
     2881 tcatctgtgg aaacattgtc tgcggtttta agaagaaggg gtagggttaa ggtgtccggg
     2941 agctccgggc aggctggaca ttctttgata ttcgggccgc gaagaatctg tgtcttcaag
     3001 gcgcgctcaa gtggtaaagg ctcaataagc attccgt
//
LOCUS       DAOQXQ010000134         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_34524_length_3035_cov_2.475503, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000134 DAOQXQ010000000
VERSION     DAOQXQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34524_length_3035_cov_2.475503"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..152
                     /locus_tag="PKH81_03010"
     tRNA            81..152
                     /locus_tag="PKH81_03010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:114..116,aa:Glu,seq:ctc)
     gene            225..1937
                     /locus_tag="PKH81_03015"
     CDS             225..1937
                     /locus_tag="PKH81_03015"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HNQ97035.1"
                     /translation="MDENLDTFERLVISLESSERQDLLKQLADKTEIRSGEGKDGTSA
                     AVLLGEASHDPAETFHEQPFLARLWFILMSLFTSVTPVGAYSASLVSSLGRKISRNCS
                     SHIDVRKRLYTDTMYRDMVRLGDVQDFFSTLLSAYENNKGDFYIVLSQLLMKNTSELI
                     ARAADPFVESYEREQTIDIRLSCLRKLDAAFMEIQEDERARMYQAAQGIEWMRRFCLV
                     PIGRILIRFNVMSDLLKGCPIDSVNEEMKALVSCFVSARKIPTLMLEAMFLFSVQDKA
                     QNIEFDIEKECASFLSTASAHLTGIREFKTTVPLADFVRFAANDTSWSPSLSEGGEDW
                     FLLFKTAWKKRFDERWGEWNRLHRSFMLRKRMIDFLGCKDLPVLTSRPWEGLWLSLSL
                     RRELSFIFLKGFFMYLYPTQVMKPLKLVLIEGDFYRRENLAEYTDSFSALEHQQAIIE
                     TFENRLTEKGDIGEGFLLIVRDKIATVKGKARLENLMLTTESEVELMISRTLAAFRSI
                     DLILGGILEVSRGGPYETLSNLAMIQGKRNEQFRKELGSVKHILREASSLLNDIEIVE
                     KESR"
     gene            1934..2692
                     /locus_tag="PKH81_03020"
     CDS             1934..2692
                     /locus_tag="PKH81_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNQ97036.1"
                     /translation="MSQVRHFSFPGAPVNPLAKGGVRYPECAITLRASGAMKPGKDDE
                     NRMRVFRSLGIAGDRVMSVTQIHSRIVHIAASSRAFTGCPEGDGVLTVHPDLVPCVTV
                     ADCMPLWLFDPVTGFFGVLHSGWKGTGILRTALDLAREEWGAQSDTIRLILGPHIRSC
                     CYTVDEERAQWFRRTVGPSCVSLDPQRQSEGSKWPWRLSLAEANRTLALSLGIQEEHL
                     LDTGVCTSCDHSFGSSRREGASCFTHMAAYIRMS"
     gene            2765..>3035
                     /locus_tag="PKH81_03025"
     CDS             2765..>3035
                     /locus_tag="PKH81_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ97037.1"
                     /translation="MSDVREQLRVLVKEALESYGTTKLNSPMEISLDAVSVETPPDPA
                     MGDVGFPMFSFAKMFRMAPPAIAADIAAVLSAHPSVASLGTVKPVG"
BASE COUNT          679 a          721 c          777 g          858 t
ORIGIN      
        1 gacgtagttc tgtgaagttc cgcgaagcgg aacttcaaga agaacggaaa aaacctcaac
       61 gaggtttttt ccgttcttca ggtcccttcg tctatcggtt aggacatgag attctcaatc
      121 ttaaaagacg ggttcgattc ccgtagggac cgtagccatc cgtaacagga tggctttttt
      181 tatctactgg tttttttacc cattctgtaa cataataaac gggtatggat gaaaatctgg
      241 acacatttga acggcttgtc ataagcctcg aatcctctga acgccaggac ttgcttaaac
      301 agcttgccga taaaaccgag attcgctccg gagagggtaa ggatggcaca tctgccgcgg
      361 ttttgttggg agaggcctct catgatcccg cggaaacctt tcatgaacaa ccctttcttg
      421 cccgcttatg gtttatactc atgtctcttt ttacctccgt cactcccgtt ggagcctatt
      481 cggcttctct ggtgtcatcc ctggggcgca aaatctcgcg aaattgttcg tctcatatcg
      541 atgtgcgcaa gcgactatat accgacacga tgtaccggga tatggtacgc ctgggtgacg
      601 tacaagattt tttttccaca cttctttctg cgtacgaaaa taacaagggc gatttttata
      661 tagtcctttc tcaactcttg atgaaaaaca ccagtgaact tattgcccgg gcggcagatc
      721 cctttgttga atcatacgag cgtgagcaaa caattgatat caggctttct tgtctgcgta
      781 agcttgatgc cgcttttatg gaaatacaag aagacgagcg agcccgtatg tatcaggccg
      841 cacagggaat tgaatggatg aggcgctttt gtcttgttcc catcggcagg atactaatca
      901 gattcaacgt tatgtccgac ttgctcaagg gctgtcccat cgattcggta aacgaggaaa
      961 tgaaggcgct tgtttcctgc tttgtctccg cgcgaaaaat tcccacgctt atgctcgagg
     1021 cgatgttttt gttttcagtt caggataagg cacagaacat agagttcgac attgaaaaag
     1081 aatgtgcctc gtttttatct acggcttccg ctcatcttac gggcatacgc gagtttaaaa
     1141 ccactgttcc tcttgcggac tttgttcggt ttgccgcgaa tgacacgagc tggagtcctt
     1201 ctctttcgga aggaggagaa gactggttct tgctattcaa aaccgcatgg aaaaaacgat
     1261 tcgatgagcg gtggggagaa tggaaccgcc tgcatcgttc ttttatgctc agaaaaagaa
     1321 tgatcgattt tctgggttgc aaagacttgc ccgtccttac aagccgcccg tgggaaggct
     1381 tgtggctttc cttgtctctg cgcagggagt tgtcctttat ttttcttaag ggttttttca
     1441 tgtatctcta tcccacccag gtaatgaagc cccttaagct tgtgcttatc gaaggagact
     1501 tttatcgcag ggaaaatctt gcggaataca ccgactcctt tagcgccctg gagcatcaac
     1561 aagcgataat agaaaccttc gagaatcgct tgacggagaa aggtgatatt ggcgaaggtt
     1621 ttttgctcat cgtcagggac aagattgcaa cggttaaagg caaggcccga cttgagaatt
     1681 tgatgctgac aactgagtcg gaagttgaat tgatgatttc ccggactctt gccgcctttc
     1741 gcagtattga tcttatactt ggcggtattc tggaggtgtc ccgaggcggt ccatacgaaa
     1801 ccctgagtaa cctggccatg attcaaggca agcgaaatga acagtttcgt aaggaacttg
     1861 gttctgttaa gcacatcctt cgtgaagcga gtagtctttt aaacgacatt gaaattgtag
     1921 aaaaggaatc gcgatgagtc aggttcgcca tttctctttc cctggcgctc ctgttaaccc
     1981 cttagccaaa ggcggcgttc ggtatccgga atgcgcgatt accttgcggg cctctggagc
     2041 tatgaagccg ggcaaggacg acgagaatcg tatgcgcgtt tttcgttctt tgggtattgc
     2101 cggagaccgg gttatgtcgg taactcaaat tcattcacgt atagtgcata ttgccgcttc
     2161 aagccgtgcc tttaccggtt gccccgaggg agacggcgta ctgaccgttc atcctgatct
     2221 agtgccctgc gttactgtgg ccgattgtat gcctctgtgg ctttttgatc cggtaaccgg
     2281 tttttttggg gtgcttcatt ccggatggaa gggaacgggt attttgcgta ccgcattgga
     2341 ccttgcccgc gaggaatggg gggcacaaag cgatactata cggttaatcc tgggtccgca
     2401 tattcgctcg tgttgttata ccgtggatga ggagagggcg caatggttta ggcgaaccgt
     2461 tggcccttcc tgtgtttctc ttgatcctca acggcaaagc gagggcagta aatggccctg
     2521 gaggctgtcg cttgcggagg cgaaccgcac ccttgccctg tcgcttggca tacaggaaga
     2581 acaccttttg gataccggcg tgtgtacctc ctgtgatcat agttttggct caagtcgtcg
     2641 tgagggtgcc tcctgtttta ctcacatggc tgcgtatatc aggatgtcct gacgcatcgc
     2701 cgctccgttg caacgctttc ctgttttgta tacactaccg ctatcagagt gaggagtgtg
     2761 gtgtatgtct gacgttcgcg aacaattgag agtgttggtt aaagaagcgc tggagtctta
     2821 tggaaccacc aagctgaact cccccatgga gatttccctt gacgcagtgt cggtagaaac
     2881 ccctcctgac cctgcgatgg gcgatgtcgg tttccccatg ttctcttttg ccaaaatgtt
     2941 caggatggcc cctcctgcta tcgccgctga tatcgcagcc gttctgtcag cacatccctc
     3001 tgttgcctct cttggtacgg taaagccggt cggtc
//
LOCUS       DAOQXQ010000135         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_34648_length_3027_cov_2.891319, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000135 DAOQXQ010000000
VERSION     DAOQXQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34648_length_3027_cov_2.891319"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKH81_03030"
     CDS             <1..1040
                     /locus_tag="PKH81_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97038.1"
                     /translation="LSAQAVVPEDVYRSVDSAMSEQGAPGLTRVLQNHAGSPWYPRLE
                     SYVIKRARQQVAANDLDQARAVSLALVDANLDNQEAVELYQSVSEAITKRDAQEKKKA
                     EQDAIAAHKAQVADSKARAEAEKTFKAVTNPTTGRKIYLDQEFNTHYREISWDLMLGL
                     MNLSYVMDSEREGLKYGLSGSAALIYRGEVFSAAADIMGDGMILALTGESGVNWSMGG
                     VASFASNNLSKKLFLRLGYATFTWDSGSENFEETAFNTPLVGLGFRDIRMGETGHFHL
                     ALDWYGGHLMDDDITVAFGADMNITFELANMQDFSIFFRMGLKDTLVLHTDGMKNDIK
                     AVLAIGVGNYE"
     gene            1033..2478
                     /locus_tag="PKH81_03035"
     CDS             1033..2478
                     /locus_tag="PKH81_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ97039.1"
                     /translation="MNKAVRTAMVTLFSLCIAVSAYTVDEQLVNRYLNAANEQYSTGN
                     MPKAFSYINIVLNSYKEEALPENIEVIAETIYYAYLEQIKNSRNSTAFNQVKEKLIAF
                     PFLSSERINRTIKIINTYEAQDIAWGADPTRAMSSGAVVGDNNPALYSTLQLQLALEE
                     SKQAALDKEKEEQEKFRAEILDTQKSAFETAFDKASEVNKSSNRAFLLVLIILSVIGF
                     TVFVVVVLNIVVNMRNAKTQNEKFVETLKAVSQLSRMPLGMAGIEALPPSFASGENLR
                     LIGSGTPESNIPPEPETVAAKSEFDGLVKKCREVGLQIDQATARKNNAKNVAEMVYKV
                     SQEMGLTQYESMVYYAVSLVYDIGFLVIDQALLMADNLTEDQKFEIRNHVKQGLAQIS
                     FVPEAYMSVFADGVLMHHENMDGSGYPEGLEGSRIPYIARVIHVAESFNALISKRSYR
                     EIYDKESAVEELRKKPGLYDPSIVEILERII"
     gene            complement(2496..>3027)
                     /locus_tag="PKH81_03040"
     CDS             complement(2496..>3027)
                     /locus_tag="PKH81_03040"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNQ97040.1"
                     /translation="HADGELDCEIPVIVSNHPDLAHIASSFHIPFFQVDPGKGKEAYE
                     NDLNGILEKYQADLVVLARYMQVMSPTFCERWSRKVINIHHGFLPAFKGAKPYHQAWQ
                     KGVKIIGATGHFATADLDQGPIISQDIIRVADTCSVDEFIRMGKDVERRVLVEAVRRE
                     LTHSIFMHDGRTFVIQ"
BASE COUNT          796 a          698 c          762 g          771 t
ORIGIN      
        1 ggcttagcgc ccaggcggtt gtacccgaag acgtgtaccg aagcgttgat tcggctatgt
       61 cggaacaagg cgctcccgga cttaccagag ttcttcaaaa tcatgcaggt tctccctggt
      121 atccccgtct agagtcctat gttattaaac gggctcgcca acaggttgcc gcaaacgatc
      181 tggatcaagc gcgcgctgtc agccttgctt tggtagacgc taaccttgac aatcaagaag
      241 ccgttgaact ctatcaatcg gtatccgaag ccattaccaa gagggatgct caggagaaaa
      301 agaaggctga gcaggatgcc atagccgcgc ataaagcgca ggtagccgac tcaaaggccc
      361 gcgccgaagc cgagaaaacc tttaaagcgg ttactaatcc cactacaggg cgtaaaattt
      421 atcttgatca ggaatttaac actcattatc gcgaaatctc ctgggatctt atgcttgggt
      481 taatgaacct gagctatgtg atggacagtg agcgcgaagg actcaagtat ggcctgtccg
      541 gcagtgcggc gcttatctat cggggtgaag ttttttccgc cgctgccgat attatgggag
      601 atgggatgat cctggcctta accggcgaaa gcggagttaa ttggtccatg gggggtgtcg
      661 cctcctttgc aagcaataac ctgagcaaaa agttgttttt acggcttgga tacgcgacat
      721 ttacatggga ttcaggcagt gaaaattttg aagagaccgc ttttaatact cccttggttg
      781 gacttggttt tagggacatt cgcatgggtg aaacaggcca cttccatctc gcgcttgact
      841 ggtatggcgg acatttgatg gacgacgata ttaccgtcgc ctttggtgcc gacatgaata
      901 ttacctttga actggccaat atgcaggatt ttagtatttt cttccggatg ggccttaagg
      961 ataccctggt gctccatacg gatggaatga aaaatgacat taaagccgta ttggcaattg
     1021 gagtaggcaa ttatgaataa ggctgtacgc acggcaatgg taaccctttt ctccttgtgt
     1081 atcgctgtat cggcgtatac ggtggacgag cagctcgtca atcggtatct gaatgcggca
     1141 aacgagcagt attcaaccgg gaacatgcca aaggcctttt cgtacatcaa tatcgtcctg
     1201 aactcttata aagaggaagc cctccccgag aacatagaag taatagccga aaccatttat
     1261 tacgcctact tggagcagat aaaaaactcc cgtaactcaa ccgcctttaa ccaggtaaaa
     1321 gaaaaactta tcgctttccc ctttttgtcc agcgagcgaa taaacaggac aatcaagatt
     1381 atcaatacct atgaggctca agatattgcc tggggtgcgg atcctacccg agccatgtcg
     1441 tcgggtgcgg ttgtagggga taacaatcct gccctgtaca gtaccttgca attgcagctt
     1501 gccctggaag agtcaaaaca agccgcgctc gacaaagaga aggaagagca ggaaaagttt
     1561 agagccgaga ttctggatac tcaaaagagc gcctttgaaa ccgcttttga taaggcaagc
     1621 gaggtgaata agtcgagtaa ccgggccttc ttgcttgtat tgataatcct ttcggtaatt
     1681 ggttttaccg tttttgtagt ggttgtattg aacattgtag taaacatgcg taatgcaaaa
     1741 acgcaaaacg agaagttcgt cgagacccta aaagcggtgt cccagctttc tcgcatgcct
     1801 ttaggcatgg ctggtatcga ggcccttcct ccttccttcg cctccggaga gaatcttcgc
     1861 cttattggaa gcggtacccc cgagagcaac ataccacccg aaccagaaac tgtcgcggcc
     1921 aaatcggaat ttgatggctt ggtaaaaaaa tgtcgcgagg ttgggcttca aattgatcag
     1981 gcaacagccc gaaagaataa cgctaaaaac gttgcggaga tggtgtacaa ggtatctcag
     2041 gaaatgggct taactcaata tgagtccatg gtctactatg ctgtttccct ggtgtatgac
     2101 ataggctttt tggtaattga tcaggcctta ctcatggcag ataatctgac cgaagaccaa
     2161 aagttcgaaa tacgcaatca cgtaaagcag ggtcttgccc agatatcctt tgtgccggaa
     2221 gcgtacatga gcgtttttgc ggacggggta ttgatgcacc acgaaaatat ggacggttcg
     2281 gggtatcccg agggccttga ggggtctcgc attccttaca ttgcccgcgt tatccatgtg
     2341 gccgaatcct ttaatgcact tatttccaag cgaagttatc gcgaaattta cgataaggaa
     2401 tctgcggtcg aggagttgag aaagaagccc gggctgtatg atccttcgat cgtggagatt
     2461 cttgaacgaa ttatctgatg cggctggtgg tcggactatt ggataacaaa ggtccggccg
     2521 tcgtgcataa agatactgtg tgtcagctct cttcgtaccg cttctaccaa aacccgcctt
     2581 tcaacgtcct ttcccattcg aataaactcg tcaactgaac aggtatctgc tactcgaata
     2641 atgtcctgag agataatggg tccctggtcc aggtctgcgg tcgcaaaatg gccggttgcg
     2701 ccaatgattt tgacgccttt ttgccaggcc tgatgatagg gcttggctcc cttaaaggct
     2761 ggaaggaatc catggtgtat gttgataacc tttctgctcc aacgctcgca aaaagtggga
     2821 ctcattacct gcatgtatct ggccagaaca actaaatcag cctggtactt ttccaatatt
     2881 ccgttcaaat cattttcgta tgcctctttc cccttgccag ggtcaacctg gaagaaggga
     2941 atatgaaatg acgaggcaat gtgggctaaa tcaggatgat tggacacaat gacgggaatt
     3001 tcacagtcga gctccccgtc tgcatgc
//
LOCUS       DAOQXQ010000136         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_34712_length_3023_cov_3.462938, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000136 DAOQXQ010000000
VERSION     DAOQXQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34712_length_3023_cov_3.462938"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKH81_03045"
     CDS             <1..1156
                     /locus_tag="PKH81_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ97041.1"
                     /translation="VGGGNKAIQVKIKSEDLDKARQTAVAIRDLFETQGADVLTDPGI
                     SMKDGLPQVNIVLDRQRMYNLGLNVYSVGQEIKANIAGTTASRFREDGEEIDITVMLA
                     ENDKTRLTDLEHIFVTNAQGMRIPVSSFARHEEGVSPIAITRENQSRLIIVSAGTVTG
                     VSITDAQKKVESMIQKAIPVDPAVRIEYGGDYEDLMKAIKQFVIIILMAVILVFAVMA
                     SQFESLLDPFIVLFTLPMTIIGIVFIYGVMGNPLNVMTAVGMLILVGVIVNNGIVLVD
                     YTNLLRKRGLSLKDACAEAGRSRLRPILMTTLTTVLGLIPMAFFPGEGSEMLQPIGQT
                     VLGGLSFGTLMTLFLMPVLYYGFNRIREKRDIRKALRAQQRLDVINGGKL"
     gene            1153..1425
                     /locus_tag="PKH81_03050"
     CDS             1153..1425
                     /locus_tag="PKH81_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97042.1"
                     /translation="MKRIEIIYSQALGEDILEALKDIPAAQFYSVIPGVRGRGYSTPK
                     MGDPVWPEINEMMIIYCDEKSEEKISAAIRKLQQIYTSEGLAFFVM"
     gene            complement(1477..1998)
                     /locus_tag="PKH81_03055"
     CDS             complement(1477..1998)
                     /locus_tag="PKH81_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ97043.1"
                     /translation="MHRQCEFCPSCGKKSWRFIDNKYWQCEACSVVLYHNVACSASVL
                     LQIDDKLLLLTRSKEPAIGLLSLPGGFIDSDERAEEAAIRECREETGLPVRDLCYLGS
                     WPNQYHYCGVLYNTCDFFFTAQTDASLSDIHLDPDECSGYVLAKSDEIDLSRIAFTSV
                     RHAVEAWMEMRSR"
     gene            complement(2003..>3023)
                     /locus_tag="PKH81_03060"
     CDS             complement(2003..>3023)
                     /locus_tag="PKH81_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNQ97044.1"
                     /translation="AGILAMDSSIILLDEPTSGLDIPSRGALLALISTLAEQGKTVIF
                     TTNRSEECRVADQVITLAPGSQSTLGKEPECSVKELNGVQKTLARLRSTARGGYGKIQ
                     SPIHCLSPLAKYLLVILSVGAALIPTSPLSLAALLFPAAILLALSRLPIARVLRRILG
                     FLPWLAVIGLIQYYFSRDVAYTASFFIRFLLIYLPLSVFSWTTSHTEIMYGMEDVLSP
                     LRVFGFPARDVSLVAGIVFRFIPLLYQEAARIICARSIRLGNARAKGGLFSRISLFAS
                     LIVPLIMRTLVRADRLAEAITARYYGAAVATRYLQVSSRRPLILVGMAFVYVLVTTFT
                     MIVWG"
BASE COUNT          862 a          751 c          755 g          655 t
ORIGIN      
        1 cgttggcggc ggcaacaagg ctattcaggt aaagataaag tctgaagacc ttgataaggc
       61 ccgacagacg gcggtcgcca tacgggacct gtttgaaacc cagggagccg atgtattaac
      121 cgatccaggc atcagtatga aagacggctt gccccaggta aacatcgttt tggatcggca
      181 gcgaatgtat aaccttgggc ttaatgtcta ttctgttggt caagagataa aggcaaacat
      241 cgcaggaacc accgcctccc gtttccggga agacggagaa gagatcgata ttaccgttat
      301 gctcgccgaa aacgacaaga cccggcttac cgaccttgaa cacatcttcg ttaccaatgc
      361 ccaggggatg cggattcccg tatcgagttt tgcccgtcac gaggaaggag tatctcccat
      421 cgcgattacc cgcgaaaatc agagccgcct tatcatcgtc agcgccggca ctgttaccgg
      481 agtatccatt acagacgcac aaaagaaagt ggagtcgatg atccaaaagg caataccggt
      541 tgaccccgca gtgcgtatcg agtacggcgg agactacgaa gatttgatga aggctatcaa
      601 acagtttgtt atcatcatcc tgatggcagt catcctggta ttcgccgtta tggccagcca
      661 gtttgagtcc cttctcgatc cgtttattgt tctgtttacc ctgcccatga caattattgg
      721 tattgtcttt atctacgggg taatgggaaa cccgcttaat gttatgacgg ccgtaggtat
      781 gcttatcctt gttggcgtta tcgttaataa cggtatcgtt ttggtagact ataccaacct
      841 gctcaggaag cgcggactat cccttaagga tgcctgtgcc gaagctggac gcagccgcct
      901 caggcccatt ttgatgacaa ccctgaccac cgtactcggt cttatcccca tggccttctt
      961 ccccggagaa ggatcggaga tgctgcaacc aatcggtcaa acagttctgg gcggactttc
     1021 gtttggaacc ctgatgaccc ttttcttgat gcccgttctc tattatggat tcaaccggat
     1081 ccgtgaaaag cgggatatta gaaaggcact acgggcgcaa cagagacttg acgtgataaa
     1141 cggaggaaaa ctatgaagcg aatcgaaata atctatagcc aggctcttgg agaagatatc
     1201 cttgaagccc ttaaagatat tcccgcggca cagttttatt cagttatccc cggcgtacgc
     1261 ggcaggggat actcaacacc caagatggga gacccggtgt ggccagagat taacgaaatg
     1321 atgattatct actgtgatga aaaatcggaa gaaaaaatct cggcagctat acgaaaactt
     1381 cagcagatat atacatccga aggtcttgcc ttctttgtca tgtaagaaaa aaaactgcgc
     1441 attacaggcg gcgtccggtt tcggtcgccg ctttttttat cgtgaacgca tttccatcca
     1501 ggcttccacc gcatgacgta cagaggtaaa tgcgatccgg gaaagatcaa tttcgtcgct
     1561 cttggcaagg acataccccg aacactcatc cggatcaagg tgaatatcgg agagagaggc
     1621 atcggtctgt gcagtaaaga aaaaatcgca ggtgttatac aaaacaccgc aatagtgata
     1681 ctgatttggc cagcttccca gataacacag gtcgcgaacc ggtaagccgg tttcttcccg
     1741 gcactctcgt atcgccgcct cttcagcgcg ctcgtcgcta tctataaatc caccgggaag
     1801 ggacagaagt cctatggccg gctctttaga tcgggtaagc aacaagagtt tgtcatctat
     1861 ttgaagcagc acactcgcgg aacaggcaac attgtgatac aagacaacag aacacgcctc
     1921 acattgccaa tacttgttgt caataaaacg ccatgacttt ttcccgcaag aagggcaaaa
     1981 ctcacattgt cgatgcatca gcctaccccc atacgatcat ggtgaacgtt gtaacaagaa
     2041 catatacaaa agccatacca acaagaatga gtggacggcg agaagacact tgtaggtaac
     2101 gggtggccac agccgcgcca taatagcggg cggtaatcgc ttcggcgagt cgatcggctc
     2161 gtactagggt tctcatgata agcgggacaa tgagagaagc gaacaaagaa atccgcgaga
     2221 acaaaccacc ttttgccctg gcgtttccca aacggattga gcgggcgcag ataatcctcg
     2281 cggcttcctg gtacaaaaga ggtataaagc gaaacacaat accggccacc aaagagacat
     2341 cacgcgcagg gaagccaaag acacgaagcg gcgacagcac atcctccatc ccgtacataa
     2401 tctcggtatg acttgttgtc caggaaaaaa ccgatagtgg aagatagatc aacaaaaaac
     2461 gaataaagaa tgaagcggta taggccacat cccgggaaaa gtaatattgg ataaggccta
     2521 tgacggcaag ccagggaaga aatcccaaaa tcctacgcaa caccctggca atgggcaggc
     2581 gggaaagagc aagaaggata gcggcaggaa aaagcaatgc ggccaaagag agcggagagg
     2641 tcggaataag ggctgctccc accgacagga ttaccagtag atattttgca agtggagaaa
     2701 gacagtggat agggctttgg attttgccgt atccaccacg ggccgtagat ctaagccgtg
     2761 ccaaggtttt ttgcaccccg ttcaactcct taacgctaca ctccggctcc ttacccaaag
     2821 ttgactgaga gccgggggca agggtaatca cctgatcggc aacacgacac tcctcgctcc
     2881 ggttagtggt aaagataact gttttaccct gctcagccag cgtggagata agagccaaca
     2941 aggctcctct actcgggata tcgagtccgc ttgtaggctc atcaagcaag atgatagaac
     3001 tatccatcgc aaggattccg gca
//
LOCUS       DAOQXQ010000137         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_34816_length_3017_cov_3.041188, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000137 DAOQXQ010000000
VERSION     DAOQXQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34816_length_3017_cov_3.041188"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKH81_03065"
     CDS             <1..949
                     /locus_tag="PKH81_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNQ97045.1"
                     /translation="QIFVNGRKINEYSLIQAIEYGSEGYFPNGSHPFALLFVTIDSSL
                     VDFNIHPAKREVRFKDSPAIHHEVSQTVRSFFRQGAVKVLAQSATGTENQLNWDLAVA
                     SPDRDTHPQTPASLYHQSVSYTDTDTVKPSSPSYGFHYLGQILGTFLVVEHRDFLYLV
                     DQHAAHERILYDGLVQEGGAIQELLVPYKIETSHSAEDLLLQNNLDELTRAGFHLENQ
                     GEGLWLATAVPSRWTGSEADLAKDLLSPRSGLSALISRIYASIACRAACKDGDVLDRD
                     TALTILTSAFALPEPVCPHGRPIWIQISRSELFHRIQRD"
     gene            complement(957..>3017)
                     /locus_tag="PKH81_03070"
     CDS             complement(957..>3017)
                     /locus_tag="PKH81_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97046.1"
                     /translation="MYLITTRKDNLNRREKIVIAVIVAVLLAIVVAVLFIMLKGPATS
                     NEDVLRHNTLSLVRSYVDKEEFDRALGLLEDLLIRDPNDKDASELLEIVLAAKKAKAA
                     LEAGKDAPNREELEQALADARRAALRIASAAEAAQKAASRIGALQDDLAKPTTGKTGS
                     KTQADSSSAELSSLDEQSRIEQDRLDAERKREAAEKALNDELAKKNKQMEQQIATVTQ
                     AIEKGKTAADAGSLRTAQEEFRKAAQAIPSEEKAFAARKYLEMAQSLYGLAEETDDST
                     IKSEALASALEYVNKSIQADPSLAEAHFVRSQIYSDQKKTDAALSEMAEASRLDPDNY
                     LYAYELGKLYYMQKRYADARTAFTRSTRLKPSFEAAFFNLGLTNRALNANREAISSFK
                     QAVSLRKTYPRAYIELARLYDRTGDTAQSIEHYRMALSQEPANISALRELAALYSKTG
                     NYVESERYFKEALALGSDDAQTNFNMATVKLELGKNSEALDFAKKAVTTDPQNAVYLY
                     TYGLAGERNGMADVALQQYTRAVTADPKYIKPRINLGVMYLDAGRLDTALNQLSVAFS
                     IENDNFEANNNLGKVYGLKGQFDKSVDHYAKAVAKHPKDADVRANLAAAYVSAGLSEK
                     ARDTYIDLIKLDGNRWDAYYELGKLHIKLDNRSAARSILEDLLKKKPDYPKAQEVRSL
                     LSTL"
BASE COUNT          744 a          757 c          663 g          853 t
ORIGIN      
        1 ccagatcttt gttaatggcc gaaagatcaa cgagtattcc ctgattcaag caattgaata
       61 cggtagtgaa ggatattttc ctaacggaag ccatcccttt gctcttttat tcgtaaccat
      121 agacagctct ttggtggact ttaatattca tccggcgaaa cgtgaagtac ggtttaagga
      181 ttctccggca attcaccatg aggttagcca gacagtacgg tcttttttta gacagggtgc
      241 agtcaaggta ttagctcaaa gcgccacagg tacggaaaac caattgaact gggacctggc
      301 agtcgcctct cctgatcgag acacacatcc tcagacaccc gcttctcttt accaccagag
      361 tgtgtcatat accgacacag ataccgtcaa accatcaagt ccttcttacg gatttcacta
      421 cctcgggcag atactgggaa cctttcttgt cgttgaacat agggactttt tgtatcttgt
      481 agaccagcat gccgcccatg agcgcatcct ctacgatggt ttagtacagg agggtggagc
      541 gattcaagaa ctccttgtac cctacaaaat agaaacctca cacagcgctg aagatctcct
      601 tctgcagaat aacctggatg agcttactcg ggcgggtttt cacctggaaa accagggaga
      661 aggactatgg cttgccaccg ccgttccatc ccgatggact ggtagcgagg cagacctggc
      721 aaaggactta ctgagtcccc gctccggttt atctgcattg ataagccgta tctatgcaag
      781 cattgcgtgc agggccgcgt gcaaggacgg agatgtcctg gatcgggaca ctgcactaac
      841 aatactcacc tcagcatttg cccttcccga accagtgtgt cctcatggac ggccaatttg
      901 gatacaaatc agccgatcag agttatttca ccgtattcaa cgagactaaa caggtgttac
      961 aaggtcgaaa gcaagcttcg cacttcttgc gcctttggat aatcgggttt tttcttaagt
     1021 aaatcctcta aaattgatcg tgcggcacta cgattatcaa gtttgatatg cagttttccc
     1081 aattcgtagt acgcatccca gcgatttcca tcaagtttaa tcaaatctat gtacgtgtct
     1141 cgagcttttt cagataaccc cgcgctaaca taggcagcag caagatttgc ccgcacatca
     1201 gcatctttag ggtgttttgc taccgcttta gcataatgat ctaccgattt gtcaaactgc
     1261 cccttaaggc cgtatacctt tcccaggtta ttattcgctt caaaattatc attttctatg
     1321 gaaaatgcaa cggaaagttg attgagcgct gtatcaagcc gacctgcatc taaatacata
     1381 accccaaggt taatccgggg ctttatatac tttggatcgg ccgtaaccgc gcgggtatat
     1441 tgctgtaagg caacatctgc cataccgttt cgctctcccg caagaccata cgtatatagg
     1501 tataccgcat tttggggatc cgtcgttacc gcttttttgg caaaatccag agcctcacta
     1561 ttttttccca attccagttt tacggttgcc atattaaaat tcgtttgagc atcatctgag
     1621 ccaagggcca gagcttcctt aaagtacctc tctgactcta cataattgcc agtcttgcta
     1681 tacaaggctg ccaactcccg caatgcagag atatttgctg gttcctgaga gagtgccatg
     1741 cgatagtgtt caatcgattg agcggtgtct ccggttcgat catataaacg cgcaagttca
     1801 atatatgctc gtggatatgt tttacgtaac gataccgcct gtttaaacga tgaaatcgct
     1861 tccctgttgg cattaagggc ccgattggtt aggcccaaat taaaaaacgc tgcttcaaac
     1921 gaaggtttaa gccgtgtgga acgggtaaat gcggttcttg catccgcata ccgtttttgc
     1981 atgtaataga gctttcccaa ttcatatgcg tataaataat tatcgggatc cagacgggaa
     2041 gcctctgcca tttcgctaag ggccgcatcc gtctttttct ggtcagagta tatctgagag
     2101 cgaacaaagt gagcttcagc caaagatgga tcagcctgaa tggatttatt cacatattca
     2161 agggcggagg caagcgcttc gctcttgatc gtactgtcat ccgtttcttc tgccaggcca
     2221 taaagagact gtgccatctc cagatatttg cgtgcagcaa aggctttttc ttcagacggt
     2281 atcgcctgtg ctgctttacg aaattcctct tgtgcagttc gcaagcttcc cgcatcagca
     2341 gctgttttcc ccttttctat agcttgggtt actgttgcga tttgctgttc catctgcttg
     2401 tttttcttgg ccagttcatc attaagcgct ttttcagcgg cttcgcgttt acgctcagca
     2461 tcaaggcgat cttgctcaat ccgggactgc tcatcaaggg atgacagttc tgccgaagag
     2521 ctgtcggctt gtgtctttga accagttttt cctgttgttg gtttggctag atcatcctgc
     2581 aaggcgccaa tacgagaagc agccttttgc gctgcttccg ccgcggacgc aatccgcaaa
     2641 gctgcccgac gagcatcggc aagtgcctgt tcaagctctt cccgattggg agcatccttc
     2701 ccagcttcca gggccgcctt agcctttttg gcggcgagta caatctccaa cagctccgag
     2761 gcgtctttat cgtttggatc gcgtatgagc aagtcttcaa gaagacccaa tgcacggtca
     2821 aactcttcct tgtcaacata cgaacgtaca agggaaagcg tattatgtct gagcacatct
     2881 tcattagagg tagctggccc tttaagcatg ataaacaaaa ccgctactac gatagcaagc
     2941 aatacagcaa caattaccgc tattactatt ttttctcgtc tattcaagtt gtccttcctc
     3001 gttgtaatca agtacat
//
LOCUS       DAOQXQ010000138         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35073_length_3001_cov_3.470468, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000138 DAOQXQ010000000
VERSION     DAOQXQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35073_length_3001_cov_3.470468"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKH81_03075"
     CDS             <1..778
                     /locus_tag="PKH81_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNQ97047.1"
                     /translation="LYTNELLPGYALSQWKNESGDRRVVLALAEKGAPKWPAGALALM
                     EDFLYHHPSRASRSRVRRPPGGEFLTGGGRVRLALEGGAPIGCSRVTYFVNGEKAVEA
                     DAEPFEGNLTLEEKGLWRLSARIEFASGEYVDTTFNPVFVSTRRDLLDGSIGVQTTPS
                     RPGQTDAWLDLSFDRLLTLDGATLAWKDSAPKSYTLEISHDGLSWRELWHGEGVDNQI
                     SFVDFNAVTARYLRISIPAGGDKPSEAFPLRRILLHGRSN"
     gene            complement(775..2376)
                     /locus_tag="PKH81_03080"
     CDS             complement(775..2376)
                     /locus_tag="PKH81_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNQ97048.1"
                     /translation="MTELLSEIHKRLTFAIISHPDAGKTTITEKLLLFGGAIHIAGAV
                     KSGKTSRAAISDFMKMEQERGISISTSVMGFEYKGRKVNLLDTPGHADFSEDTYRGLS
                     AVDSALMVIDSVKGVEERTRQLCVVCRMRATPIMTFINKLDREGKDPMELLDEVEREL
                     AVQVRPITWPIGQGKLFKGVYNLLDHTLYLFRPGEDQLPVDTVSISSLSDPLLDEKVG
                     ASLARKLRDDVEMISGVYPSFDRDEYIQGEATPVFFGSALNNFGVRELLDHLTTLAPY
                     PTPKEAEERLVAPEESKFSGFVFKIHANMNPRHRDRIAFLRVVSGKFERNTMYYHVRS
                     GKSFRTANPTAFMSQDREIIDEAWPGDIIGLHDTGTFKIGDTLTEGESVSFKGIPSFA
                     PQIFRSIRNADPLKEKQYHKGLDQLAEEGVVQIFSKIHQPNARVLGVVGQLQLEVLKS
                     RLINEYAADCAYEPIDMTIAHWITSDDPKILKTFVDANTRHVLVDIRGNYVYVSDSEW
                     AVNRVRQNNPAIRFYATSEMIDKRA"
     gene            2781..>3001
                     /locus_tag="PKH81_03085"
     CDS             2781..>3001
                     /locus_tag="PKH81_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97049.1"
                     /translation="MTFGLWYLIFLFMTVTIHEQRLGRFSIDVPYNQPLIDALKRVPG
                     HSWDPESRLWSVPAKPWVAQKLLKELYATG"
BASE COUNT          790 a          736 c          735 g          740 t
ORIGIN      
        1 cttgtataca aatgagcttt tgcctggtta tgccttgagc caatggaaaa atgagtcggg
       61 agatcgtcgg gtagtcttgg ctcttgcaga gaaaggtgcg ccaaaatggc ccgccggagc
      121 ccttgcgctc atggaggatt ttttgtatca ccatccttct cgtgcctccc gttcccgtgt
      181 acgcaggccc ccaggaggag agtttctaac tggcggagga agagttcgcc ttgcccttga
      241 gggaggggcc ccgatcggtt gttcgcgagt aacgtacttc gttaatggag aaaaagcagt
      301 tgaggcagat gcagagccgt ttgaaggaaa tttaacctta gaagaaaaag gcctgtggcg
      361 tctttccgcc agaatagaat tcgccagcgg cgagtacgta gacactacct ttaatcctgt
      421 gtttgtcagt acgcgaagag acttgttgga tggaagcata ggcgttcaaa caacaccatc
      481 gcgaccggga cagacggacg catggcttga tctttccttt gatagattac ttaccctgga
      541 tggtgcaacc cttgcatgga aagattcggc cccaaagtcc tacacgcttg aaatatccca
      601 tgacggtcta agttggcgcg agctgtggca cggtgaggga gttgataacc aaatcagctt
      661 tgtcgatttt aacgctgtaa cagcacgcta cctgaggatt agtattcctg ccgggggaga
      721 caagccaagc gaagcattcc cgctcaggcg catactcctc cacggcagaa gcaactaagc
      781 tcgcttgtcg atcatctcgc ttgtggcata aaagcggata gccgggttgt tttgacgcac
      841 acggttaact gcccactctg agtccgacac atatacataa ttaccccgga tgtctaccaa
      901 gacatggcga gtgttcgcat ctacaaaggt ttttagtatc ttagggtcat cagaggtaat
      961 ccagtgcgca atagtcatat cgatgggctc ataagcacag tctgctgcat actcattgat
     1021 cagccggctc ttaagtacct caagttgtag ctgccctacc accccaagaa ctcgcgcatt
     1081 gggttgatga atcttggaaa agatttgaac aacaccttcc tcggcaagct gatcaagccc
     1141 cttgtgatat tgcttttcct taagaggatc cgcgttgcga attgatcgga aaatctgagg
     1201 agcaaaactt ggaatcccct taaaggaaac cgactctccc tcggtaaggg tgtcgccaat
     1261 cttgaatgtt cctgtgtcat gaagaccgat aatgtctcct ggccaagctt catcaataat
     1321 ttcccggtcc tggctcataa aggcagttgg attagcggtg cggaaactct tgcctgaacg
     1381 aacgtggtag tacatcgtgt ttcgttcgaa tttaccggat accacacgta aaaatgcaat
     1441 acggtcgcgg tgtcgaggat tcatgtttgc gtgaatcttg aacacaaatc cggagaattt
     1501 ggattcttct ggagctacca gtctctcttc cgcttcttta ggtgtgggat acggggctaa
     1561 agtggtcagg tgatcaagaa gttcgcgaac cccaaaatta tttagggctg acccaaaaaa
     1621 cacaggggta gcttccccct ggatatattc atcacgatca aaagaaggat agaccccgct
     1681 aatcatctcc acatcatcgc gaagcttacg tgcaagagag gcgccgacct tttcatcgag
     1741 aagcggatct gaaagactac taattgaaac cgtgtccacc ggcaattgat cctcgccagg
     1801 gcggaataaa tacagagtat ggtcaagaag attgtacacc cctttaaaca gcttcccctg
     1861 accaataggc caggtgatgg ggcgtacttg tacggcaagt tccctttcta cctcgtctaa
     1921 aagctccatg ggatccttgc cctcgcggtc gagcttgttg ataaaggtca taataggggt
     1981 tgcccgcata cgacagacca cgcagagctg gcgtgtacgc tcctcgacac ccttgaccga
     2041 atcaataacc ataagagcgc tgtcaaccgc ggaaaggccg cgataggtat cctcagaaaa
     2101 gtcagcgtgg ccgggggtat ccaaaagatt taccttgcgt cccttgtatt caaagcccat
     2161 aaccgaggtg ctgatagaaa taccccgttc ctgctccatc ttcataaagt cagaaatagc
     2221 ggctctactg gttttaccgg acttgaccgc cccggctata tgaatggctc ctccaaacag
     2281 aagcagtttt tcggtaattg ttgttttacc agcgtcaggg tgagaaataa tagcaaaggt
     2341 tagtcgtttg tgaatctcgg aaagaagttc ggtcatagaa agcagaatat cctaaaacct
     2401 gtgccagttt ctactattag aagggttttt tttggttgac agcttccctc aagggtcgta
     2461 ctgtggtaca taagccaaaa aagcacatga cgacaaccgt cccttatggc agtaacaaca
     2521 atgagacagc ggaagcgagt agaaaacctt ccccgcttgg acacggtgtt tcggcccgaa
     2581 ccaacaactg cctctccatg ccgactcacc ccgtattgcc ctttgtggcg aaatatagga
     2641 tctatggtag gcgggcatta tcagtaaggc acagtaaaaa tgaacagcga caaaccagac
     2701 atagagaaaa tccttgaggc ccttgagcgg gtttttcgag acggatagcg caacatcttt
     2761 gaaagaaaag aaatctttat ttgacattcg gcctctggta tcttattttt ttatttatga
     2821 cagtcactat acacgaacag agactgggca gattttccat tgatgtacct tacaaccagc
     2881 cccttatcga tgcgctaaag cgcgttcccg gccattcctg ggatcccgaa tcccggctat
     2941 ggagtgttcc tgccaagccg tgggttgccc aaaagctgct caaagaactg tatgctaccg
     3001 g
//
LOCUS       DAOQXQ010000139         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35095_length_3000_cov_2.576910, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000139 DAOQXQ010000000
VERSION     DAOQXQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35095_length_3000_cov_2.576910"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKH81_03090"
     CDS             <1..442
                     /locus_tag="PKH81_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNQ97050.1"
                     /translation="VVALMAAIEHFLGWDEVQQAVLIGAGNLGTALAGYQEFQFHGLN
                     ICAAFDQYPAKIGTNIHGVPVFSLETLSHQVSVLGASIAVLTVPSPYAQEVTDQLVQS
                     GITAIWNFTNVKLKVPDNVIVQREDLTSGYAMLCVMMNARKNDE"
     gene            435..1475
                     /locus_tag="PKH81_03095"
     CDS             435..1475
                     /locus_tag="PKH81_03095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ97051.1"
                     /translation="MSDEGRDEAERKRRQAELFSNRLDKQWKHLKKWAKRTEVSAWRL
                     YDRDIPEIPLAVDVYVDCTTACTALHIALYDRPYEKDEAAEELWMAAMKEVASKICAV
                     PLERVYTKIRKKQRGEDSQYEKIATKPFIMTVREGEGKFLVNLSSYVDTGLFLDHRPG
                     RALVGKNAKAKRVLNLFCYTGAFSIHAALGGASSVTSVDLSKTYLSWAVENFRLNGLE
                     AAFPSPDKSKWRFIHSDVRAFLEQAKKKGERWDTIVCDPPTFSNSKRSPDTFDVNRDW
                     LSLCQACLSVLAAGGTLYFSTNSRSLKFDGELLKQGQSNLSIQDISEASIPEDFRNKK
                     IHRMWTITVEKA"
     gene            complement(1456..>3000)
                     /locus_tag="PKH81_03100"
     CDS             complement(1456..>3000)
                     /locus_tag="PKH81_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ97052.1"
                     /translation="VDPVLSLHHVWEVPIKTFFGQASLYMFFVYGSICVFGLEPAYRW
                     MKRKDVPLILRGLVYMSIILCMECTLGWVLRFFTGYDIWIYYGPGTLFTYTSLAIAPM
                     WFICGLISENVISLIDSFDAFKMETYGLVPGKKGKRNRIVFTSDIHIGPRAQDEGNAG
                     WFYGIYEVYLTIFLYKVSMDKRVKELVFVGDLFDTWLYPPDQKPLSVAQTIELWKDSL
                     FMAPLKRCIARLDTVWYITGNHDMGTRQEELSGLETEGKNLVVVSPQEFALKHTLSCG
                     TALAVEHGHDADFFNAPDDEKDSVQGFPFGYFVARLVSAADENQSVGSALDKALRGAY
                     EKVLVAEFSAKHDETADHRAGRFFIRVFTDALVSWVNAKRPEGDRIGNNTIIKMPSGY
                     RDVTVGEVKQHYSSLLAQWLESGKTSLLVAAGRGGLDRYARKKFGERMWRLWFVRLFS
                     WRNPDLIVIMGHTHFAKIEYIMDREKQGIYANTGCLCKNGKQSGRYCVVLIDGERRCR
                     VKLSPL"
BASE COUNT          814 a          771 c          696 g          719 t
ORIGIN      
        1 tgttgttgcc cttatggccg cgattgaaca ctttcttgga tgggacgagg tgcagcaagc
       61 ggtccttatc ggcgcaggta acttgggtac agcccttgcc gggtatcagg aattccagtt
      121 tcacggactt aatatctgcg ccgccttcga tcaatacccg gctaaaatcg gtacaaacat
      181 ccacggagtc cctgtttttt ccctggaaac cctttctcat caggtgtccg ttttaggtgc
      241 aagcattgcg gtgttaacgg ttccctcgcc ctatgctcaa gaggttaccg atcagcttgt
      301 tcaatcgggc attaccgcta tatggaattt taccaacgta aaactcaagg tacccgacaa
      361 cgttatcgta cagagggaag accttacctc aggctatgcc atgctctgtg ttatgatgaa
      421 cgcgagaaaa aacgatgagt gatgaagggc gcgacgaggc tgagcgaaag agacgacagg
      481 cggagctttt ttccaaccga ctggacaagc aatggaaaca cttaaaaaaa tgggcaaaaa
      541 gaactgaggt aagcgcctgg cgcctgtatg atcgcgatat ccccgagatt ccccttgcag
      601 tagatgtgta tgtcgactgt actaccgcgt gcacagcctt acatattgcc ttgtacgatc
      661 gcccgtatga aaaggatgag gcggcggaag agctctggat ggcggcaatg aaagaggtgg
      721 cttccaaaat ttgcgccgtt cctcttgagc gggtatatac caagatccgg aaaaaacagc
      781 gcggcgaaga ttcccaatac gaaaagatag cgacaaaacc ctttatcatg acggtgcgcg
      841 aaggcgaagg aaaattcctt gtcaatctat cctcatatgt ggataccggt ttatttttgg
      901 atcaccgccc gggcagagcc cttgtgggta aaaacgcgaa agccaaacga gtccttaatc
      961 ttttttgcta cacaggcgca ttttccattc atgccgccct gggcggagcg agctcggtta
     1021 cctcggtgga tctttccaaa acctatcttt cctgggcagt tgagaatttc agattaaacg
     1081 ggttggaagc agccttccct tccccggata aaagcaagtg gcgttttatc catagcgatg
     1141 ttcgcgcctt tttggagcaa gcaaaaaaaa agggagagcg ctgggacact atcgtctgtg
     1201 atccccctac cttttccaac tctaaacgtt cgccagacac tttcgacgta aacagggatt
     1261 ggctctccct gtgtcaagcc tgcctttccg tccttgccgc aggagggacc ctgtattttt
     1321 ctaccaattc ccgttcactc aaattcgacg gggagttatt aaagcaaggg caatccaatc
     1381 tatccatcca ggatatctcg gaagcttcaa tcccggaaga ttttcgcaac aagaagatac
     1441 atcgcatgtg gactattaca gtggagaaag cttaactcga caacgccgct cgccgtctat
     1501 caacaccaca cagtatctac cagactgttt gccgttttta caaaggcatc cggtgttcgc
     1561 atatattccc tgcttttccc gatccatgat gtattctatt tttgcaaaat gcgtgtgacc
     1621 cattattacg attagatccg ggttgcgcca gctaaacaga cgcacaaacc acagccgcca
     1681 catccgctcg ccgaatttct ttcgcgcgta tcggtctagg ccgccacgcc ctgcggcaac
     1741 taacagagac gtctttccgc tttccagcca ctgggcaagg agcgaagaat agtgttgctt
     1801 aacctcgcca accgttacat cgcgatagcc ggagggcatc ttgataatgg tattattgcc
     1861 gattcgatct ccctccggtc tctttgcgtt aacccacgaa acaagggcat ctgtaaagac
     1921 ccgaataaaa aaccttccag ctcgatggtc ggcagtctca tcgtgtttgg cggaaaactc
     1981 cgctaccagg actttctcgt aagcgccgcg caaggcctta tcaagggcgc tacctaccga
     2041 ttggttttcg tcggctgcag atacaagacg ggcaacaaaa tatccaaagg gaaaaccctg
     2101 aacagagtct ttctcgtcat cgggagcgtt aaaaaaatca gcgtcgtgtc catgctccac
     2161 ggcaagggct gtgccacaag agagcgtatg cttcaaggca aactcctggg gacttactac
     2221 gacaagattt tttccctcag tttccaaacc agagagctct tcttgccggg ttcccatatc
     2281 atggttgccg gtgatgtacc agaccgtatc aaggcgtgcg atacatcgtt taagaggagc
     2341 cataaaaagg gaatctttcc ataattcaat cgtctgtgca accgacaggg gtttttgatc
     2401 cggtggataa agccaggtgt caaaaaggtc tcctacaaaa acgagttctt taacgcgctt
     2461 atccatagaa accttgtaca agaaaatcgt tagatacacc tcgtagattc catagaacca
     2521 gccggcattt ccttcatcct gcgcgcgagg gccaatgtga atatcgctgg taaacacgat
     2581 gcggttccgt ttccccttct ttcccggaac caagccatag gtttccatct tgaatgcgtc
     2641 aaaggaatcg ataaggctta tcacgttttc cgagataaga ccgcagataa accacatggg
     2701 ggcaatggca aggcttgtgt acgtaaacaa ggttcccggc ccataataaa tccaaatgtc
     2761 gtatccggta aaaaaacgga gaacccatcc aagagtacac tccatacata aaatgatgga
     2821 catgtataca agaccccgca ggataagggg aacatccttc cgcttcatcc accggtaggc
     2881 aggctcaagg ccaaaaacac agattgagcc atagacaaaa aacatataca aactagcctg
     2941 tccaaaaaaa gtttttatgg gaacttccca tacatgatgc aggctcaata caggatcaac
//
LOCUS       DAOQXQ010000140         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35214_length_2992_cov_1.749745, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000140 DAOQXQ010000000
VERSION     DAOQXQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35214_length_2992_cov_1.749745"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKH81_03105"
     CDS             complement(<1..224)
                     /locus_tag="PKH81_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97053.1"
                     /translation="MRIKTLVLLLLVSALSGLFAQSSEDWYQNKPIRMISFEGLKGVE
                     KSELDGLFGSYLGKPFSDERYWEILQKMYAL"
     gene            complement(265..>2992)
                     /locus_tag="PKH81_03110"
     CDS             complement(265..>2992)
                     /locus_tag="PKH81_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNQ97054.1"
                     /translation="ILLSAKGNEQGIDITDISLSALSMPVERAISIKRDGDDLLFDST
                     LTVNAIPYSVSGMYTNGNLSLYGDYNLAVSAIHDPITGISGAFRVSGFPIPVGTALLS
                     VNLDAGFVAGGSEPWRIVIRDGLIEELNGYARLSSRLVFSGTIESGGAHFHELVLTDE
                     YSTVSGFARVNLATSLDGISQYQGQLRLESENGLESYALDAQITNAAELFIDASLAIN
                     GFPLMRVMADQDQQNGISAVITCSGTPSTLMASANLGLLSVKMNGHDLHAQGAAMLED
                     RAVSIYGAGLFWNGQNLTGIERSFSPDTGEGLVSSAYNGVLDESGINGSLSFRFVPDT
                     AVDDNADNSLVAYLSRYTATLSIQTLTWNTIDLTEPFVVSLVHEPGITALYAGENEVI
                     TGFLLEDGTFTLHVGEGLPIRFSADGLMRDGSIAVTVDGIRLEPSQLWPLAGIDVIAF
                     DSGVIEGSLIISGLLSDPDFNGMLYPSGIVVRSPDYAAEVFTQEPFVIRAEGKTLSAR
                     DVRVTSSEGDLTASCTAYFDRWIPNRCELSVRTVEDDSVLAATENEYFKAEGSVDCDL
                     DMSFSREEIRIAGNAGFENGFFTIIFSEFSKQYEQSQSISAPVYLDISLDLGKKVEFR
                     WPTDDFPILRGLIQADEPVRITYDSSTNRFSLKGQAQLKGGEVFYIKRSFYLRQGSIV
                     FNENQDIFDPLISMRAEIREMDEEGEPVRIILSVENQGLTSFTPLIYSEPPKAYADLM
                     TLLGQAASGDSSRDSILRTTVITASDILAQMGFFRGTESWIRDMLHLDIFSIRTLLLQ
                     NAIFGQSIQANPDAKMTVGNYFDNTTVYMGKYFGSAVYADALMHFSYYDQKTAENSDD
                     KKTIYGNLLFQPELGLEFTTPFFLVRWGISPESYDTFFVGDTSITLSWKFSY"
BASE COUNT          925 a          710 c          630 g          727 t
ORIGIN      
        1 agggcgtaca tcttttgaag tatttcccag tagcgttcat cgctaaaggg ctttccaaga
       61 taagagccaa aaagcccgtc aagttccgat ttttcaacac ccttcagtcc ctcaaagctg
      121 atcatcctga taggtttatt ctggtaccag tcttccgatg actgcgcgaa aagaccggac
      181 agcgccgata ccagcaaaag aagaacaagg gttttaatgc gcattacaaa agctccttaa
      241 gcgttgacta tagcatattt acggtcagta tgaaaacttc cacgagagcg ttatagaggt
      301 atctccgaca aaaaaagtgt cgtaactttc cggggaaatt ccccatctaa ccagaaaaaa
      361 cggggttgta aactcaagtc caagttcggg ttggaacaaa agattgccat aaattgtttt
      421 tttgtcgtca ctattctccg ccgtcttttg atcataataa ctgaaatgca tcaaagcgtc
      481 tgcataaaca gctgatccga agtattttcc catgtaaaca gtcgtattgt caaaatagtt
      541 accaacagtc attttggcat ctggattagc ctgaatgctt tgaccaaaga tagcattttg
      601 caacaacaag gttcgaatag aaaagatatc aagatgcaac atatcccgaa tccagctttc
      661 tgttccccgg aaaaaaccca tctgagcgag aatatcggat gcggtaatga cggtagtccg
      721 taaaattgag tcgcgagatg aatctccaga ggcagcctga ccaagcagtg tcatcaaatc
      781 ggcataggcc tttggaggtt ctgagtaaat caatggagta aaactggtta atccctgatt
      841 ttctaccgag aggatgatac gaaccggctc accctcttca tccatttccc tgatttcagc
      901 acgcattgat atcagtggat caaaaatatc ttgattctcg ttaaagacta tacttccctg
      961 tctgagatag aaacttcgtt tgatatagaa cacttctcca cccttcagct gggcttgtcc
     1021 cttaagagaa aaacggtttg tcgaagaatc ataggtaatt cgtaccggtt catctgcctg
     1081 aatcaatcca cgtaaaatag ggaaatcgtc cgtaggccaa cgaaattcca cttttttacc
     1141 aagatcaaga gaaatatcta aataaaccgg ggctgagata gattgagact gttcgtattg
     1201 cttggaaaat tcagagaaaa taattgtaaa aaacccgttc tcaaaacctg cattccctgc
     1261 aattcgaatt tcttctcgag aaaaggacat atcaagatcg cagtcaactg aaccttctgc
     1321 cttaaagtat tcgttttcgg ttgcagccag taccgaatca tcttccaccg tgcgaacaga
     1381 caattcacat cgattaggta tccatcgatc aaaataggca gtacaagaag cagtaagatc
     1441 tccttcgcta ctggtaaccc tgacatcgcg agcactcaag gtttttccct ctgcacgaat
     1501 cacaaatggt tcctgagtaa atacctctgc tgcatagtcc ggtgatctaa ccacgatacc
     1561 ggaaggatag agcataccgt taaaatccgg atcacttagc aagccggaaa taataaggga
     1621 gccttcaatt acaccagagt cgaacgcgat aacatctatt cctgccaaag gccataactg
     1681 tgatggctca aggcgtatcc cgtcaacagt aacggctatt gaaccgtcgc gcatcaagcc
     1741 gtcagcagaa aaacgtattg ggagcccctc tccaacatgc aaagtaaaag tgccatcttc
     1801 aagaaggaat ccggtaataa cttcgttctc tcccgcatac agcgcagtta ttcccggctc
     1861 atgcaccaat gagacaacaa agggctccgt aaggtcgata gtattccagg tgagcgtttg
     1921 tattgaaagg gtcgcagtat agcgagaaag atatgccacc agtgagttat ccgcattgtc
     1981 atcaaccgca gtatctggaa caaaacgaaa agacagagaa ccgtttatcc ctgattcatc
     2041 aagaacacca ttataagccg agcttaccag accttctccc gtatctggtg aaaacgacct
     2101 ttctataccg gtaaggtttt gaccgttcca gaatagtccg gccccatata tagaaacagc
     2161 acggtcttcg agcatagcgg cgccctgcgc gtgtagatca tgaccgttca tcttaaccga
     2221 aagtaagccg agatttgcag aagccatcag ggtagatgga gtgccggaac aggtaataac
     2281 cgcggaaatt ccgttttgct gatcctgatc tgccatgacg cgcataagtg gaaacccatt
     2341 aatcgcaagg ctcgcatcaa taaacaactc ggcagcgttg gtaatctgcg cgtcaagcgc
     2401 atatgattcc aggccatttt ctgattcaag tcgaagttga ccctggtact gactgattcc
     2461 atcaagggaa gtggcaagat tcacccgagc aaaaccggag acagtggaat actcatcggt
     2521 aagtaccaac tcatgaaagt gggcgccacc gctttctata gtcccggaaa acaccaatct
     2581 ggatgaaagt cttgcatatc cgttaagctc ttcaataaga ccatcgcgaa tgacaatgcg
     2641 ccagggttcg ctgccgccgg caacaaagcc cgcgtcaaga ttcaccgaca aaagagctgt
     2701 accgacagga atgggaaaac ctgaaacacg aaatgctcca gaaataccag taataggatc
     2761 atgaatagcc gagaccgcga ggttgtaatc cccatataaa gaaagattac cgttagtgta
     2821 cataccagat accgaatatg gtatggcatt aaccgtaagg gtactatcaa acaagagatc
     2881 atccccatcg cgctttatcg aaattgccct ttcaacgggc atcgaaagcg cagacagaga
     2941 aatatcggtg atatctatgc cctgttcatt tccttttgca gatagaagaa ta
//
LOCUS       DAOQXQ010000141         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35338_length_2984_cov_3.190850, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000141 DAOQXQ010000000
VERSION     DAOQXQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35338_length_2984_cov_3.190850"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..645)
                     /locus_tag="PKH81_03115"
     CDS             complement(10..645)
                     /locus_tag="PKH81_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97055.1"
                     /translation="MYFPLVLIVPICFVYYSAGTRFAGQSAYRLSYLSGALAALPVIL
                     MDLICGSLIPSRTPYFIVKLIDVYVSGILLPLIVAPIILYFVAKVPIERRIRRIVPQL
                     FGILTVYLPWFMFSDFMKPDFWPVVILPALFLALVFMVDICINTYLGSIRLGPTLEGL
                     LFALIPAGCASFCVMFAVTIWYLNKGSLLLWIVTLIVPLATLFIRLKKYRA"
     gene            782..1303
                     /gene="infC"
                     /locus_tag="PKH81_03120"
     CDS             782..1303
                     /gene="infC"
                     /locus_tag="PKH81_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ97056.1"
                     /translation="MADNKGLRINEQIRVREVRLIDDSGEQKGIVPTLEALKMARELS
                     LDLVEVAPQANPPACKILDYGKYRFEMEKKLRDSKKKQKLQVLKEIRMQPKIDDHDLD
                     FKSKHVKEFLAGGDKVKVTIRFRGRELAHTELGLVVLKDVLSKLGDDYVVEKQPSMEG
                     RFMSMTLAPKAKK"
     gene            1319..1519
                     /gene="rpmI"
                     /locus_tag="PKH81_03125"
     CDS             1319..1519
                     /gene="rpmI"
                     /locus_tag="PKH81_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ97057.1"
                     /translation="MPKMKSKRCAAKRFSFTASGKVKYKKMNLRHILTKKTAKRKRNL
                     RQGGILSEADAAKIRKQLLPYG"
     gene            1545..1904
                     /gene="rplT"
                     /locus_tag="PKH81_03130"
     CDS             1545..1904
                     /gene="rplT"
                     /locus_tag="PKH81_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ97058.1"
                     /translation="MPRAIDGSKRKEHRKKILKLAKGFRGRRGTNFKAAKDAVVKALV
                     HGYVSRRDRKGDMRSLWITRINAAVRTEGLSYSRFIQGLTKAGVIINRKALSNMAIED
                     PDAFKAVVTTAKKALEA"
     gene            1906..2307
                     /locus_tag="PKH81_03135"
     CDS             1906..2307
                     /locus_tag="PKH81_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNQ97059.1"
                     /translation="MLNLDQVRLLENRVEKAVGKIQSLGQENTRLRNELSTLQTRVVE
                     LESLVNTFKDDQGRIEEGILNALDRLSAFEDSVFNTEISTQPDTNIEVENPVYPVPQT
                     DNSLDIQPEWQDEPIIEKTDVSSADGQMDIF"
     gene            2317..2613
                     /locus_tag="PKH81_03140"
     CDS             2317..2613
                     /locus_tag="PKH81_03140"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNQ97060.1"
                     /translation="MAKGSLQLDLLGTSFAIQADERPEYLEGLYAHLKKTIKQIETTS
                     SITDPLKIAIIAGVLISDELYKEKQKNHREPTSLDLDEAEKIALGMISRIDQVI"
     gene            2613..>2984
                     /locus_tag="PKH81_03145"
     CDS             2613..>2984
                     /locus_tag="PKH81_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HNQ97061.1"
                     /translation="MAKDSEASLILWVDADSCPRPARDLVSRTSMRLSLPLIYVANHQ
                     IPAPESPHFTMIVCQTHQDAADDYIVEHARPNDLVITRDIPLAKRLVDKNITVINDRG
                     VVYTEKNISERLSMRNFMMDLY"
BASE COUNT          993 a          630 c          647 g          714 t
ORIGIN      
        1 cggttttttt tatgcacgat actttttaag gcggataaac aaagtcgcca gtggaacaat
       61 cagcgtaaca atccataaca aaagagatcc cttattaagg taccatatcg taaccgcaaa
      121 catgacacag aaagaggcac atcctgccgg aatcaaggca aaaagaagtc cctcgagtgt
      181 tggaccaaga cgaatagaac ccaaataggt gtttatgcaa atatctacca taaaaaccag
      241 cgctaaaaaa agcgctggta gaattacaac cggccagaaa tccggcttca taaaatctga
      301 aaacataaac cagggaagat ataccgttaa aattccgaat aactgaggaa caatacgacg
      361 aattctccgc tcaattggca ccttagctac aaaatagagg ataatgggag caacaattaa
      421 gggtaataaa atccctgaaa catacacgtc aatcaatttt acaataaaat aaggggttct
      481 tgacggaatt aacgaaccac atatcaaatc cataaggata acaggaagtg ctgccagagc
      541 tcctgagaga tacgaaagac gataagctga ctgaccggca aatctggtac cagccgaata
      601 atacacaaaa caaatcggaa caatcagtac aagtgggaaa tacataggga taaattatca
      661 tgaggcgaga gcctttgcaa gtcttgacga ttattgacat aaaataacaa accttgctat
      721 agtagggttc gtaacttttt ttgagaacag aagtgctaat tacggcacct aagggggatc
      781 gttggcggac aataaaggat tacggattaa tgaacaaatc cgtgtacgcg aagtcagact
      841 tatcgatgat agcggtgaac aaaagggtat agtccctacc cttgaggcac tgaaaatggc
      901 tcgagaattg agtcttgact tggtagaagt tgcgccgcag gcgaatccac cggcttgcaa
      961 gatccttgat tatggcaaat atcgtttcga aatggaaaag aagctccgtg attccaaaaa
     1021 gaaacaaaaa ttgcaggttc tcaaggaaat tcgcatgcag cctaagatcg acgatcatga
     1081 tctcgatttt aagtcaaagc atgtaaagga atttcttgct ggcggagaca aggttaaggt
     1141 tacaatccgg tttcgcggac gcgaactggc tcataccgaa cttggccttg tagttcttaa
     1201 agatgtgtta agtaagcttg gggatgatta tgttgtcgaa aaacaacctt ctatggaagg
     1261 tagatttatg tcgatgacgc tcgcacccaa agcaaaaaaa tgacgaggta gtggtatcat
     1321 gccaaagatg aaaagcaaac gatgcgccgc aaagcgcttt tcctttaccg caagcggaaa
     1381 ggtcaagtac aagaagatga atcttcgcca cattcttacc aagaagacag caaagcgcaa
     1441 gagaaatctt cgccagggcg gaatcctctc cgaggcagat gccgcgaaga tccgcaaaca
     1501 acttttgccg tacggttaat tatcagttat tcaggagata aagaatgcca agagcgatag
     1561 acggtagcaa acgaaaagaa caccgtaaga agattcttaa acttgcaaag ggtttccgcg
     1621 gtcgccgggg aaccaacttt aaggcagcta aagacgctgt tgtaaaagca cttgtacatg
     1681 gctacgtttc acgccgtgat cgcaagggag acatgcgctc tctgtggatt acccgtataa
     1741 atgcggcagt tcgtacagaa ggtctttcat attcacgctt tattcaggga ttaaccaagg
     1801 ctggtgttat catcaaccgc aaggcccttt ccaacatggc tattgaggat ccagatgctt
     1861 ttaaggcagt agttacaaca gccaaaaagg ccctggaagc ttaatatgct taatcttgat
     1921 caggtccgtc ttcttgaaaa cagggttgaa aaagccgtcg gcaaaataca gtctctcggc
     1981 caggaaaaca cccgtttacg caacgagctt tctaccctac aaaccagagt tgttgaacta
     2041 gaaagccttg taaacacctt caaggatgat cagggccgga tcgaagaagg aatcctcaac
     2101 gcacttgatc gtttaagtgc gtttgaggac tctgtattta acacagagat cagtactcaa
     2161 cccgatacta atatcgaagt agaaaacccg gtgtatccgg ttccacaaac agataattcc
     2221 cttgatattc aacccgaatg gcaggatgag ccgattattg aaaaaaccga tgtttcttcc
     2281 gccgacggtc aaatggatat cttttaggat ataactatgg caaaaggcag tctgcaatta
     2341 gatctgctcg gcacttcttt tgccatccag gccgatgaac gacctgagta tcttgaaggt
     2401 ttatacgcac atcttaaaaa aactatcaag caaatagaaa ctacttcttc gatcacagac
     2461 cccctaaaaa tagctattat cgccggcgtc ctgatatcag atgaactata taaagaaaag
     2521 caaaaaaacc atcgtgaacc cacatcactt gatctggatg aagcggaaaa aatcgctctt
     2581 gggatgattt ctcggattga tcaggttatt taatggccaa agatagcgaa gccagtctta
     2641 ttctttgggt agacgccgac tcttgtcccc ggccagctcg tgatcttgta tctagaacat
     2701 ccatgcggtt atccttgccc ttgatatatg ttgccaacca tcagatccca gccccagaaa
     2761 gtcctcattt tactatgatt gtatgtcaaa cccatcaaga tgcggccgac gattatatcg
     2821 ttgaacatgc acgacccaac gatcttgtta ttacccggga cattcctttg gcaaaaagac
     2881 tggtagataa aaatataacc gtcataaatg accgaggtgt ggtatatacg gaaaaaaata
     2941 ttagtgaacg gctttctatg agaaatttta tgatggattt atat
//
LOCUS       DAOQXQ010000142         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35563_length_2971_cov_2.708848, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000142 DAOQXQ010000000
VERSION     DAOQXQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35563_length_2971_cov_2.708848"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..451
                     /locus_tag="PKH81_03150"
     CDS             239..451
                     /locus_tag="PKH81_03150"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97062.1"
                     /translation="MSPNIDDDPIDFYSFNGNEAVYDVIYHPDKTRMLKRAEKAGCRV
                     CNGYTMLAYQAYLQYRLFTGVDYAEE"
     gene            438..1166
                     /gene="rpiA"
                     /locus_tag="PKH81_03155"
     CDS             438..1166
                     /gene="rpiA"
                     /locus_tag="PKH81_03155"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNQ97063.1"
                     /translation="MPKNECTLAEQKTLAARHAVDDLIRRGELRSNMKLGLGTGSTAM
                     PAVARIAEHIREGNLTGIRAVATSFQTTIACEDLGIPVFSLNSKEISGELDLAIDGAD
                     EISPAKHLIKGGGAALLLEKIVAYNSALFVIVADSSKRVTTLGTRFALPVEVISEARV
                     SVTRALASLGAQALLREGVRKAGPVVTDNGNLILDCVWKEAVDAYQMEEAINQIVGVV
                     ENGFFTRTSPIVYAAANTGIVERV"
     gene            complement(1178..2119)
                     /gene="era"
                     /locus_tag="PKH81_03160"
     CDS             complement(1178..2119)
                     /gene="era"
                     /locus_tag="PKH81_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNQ97064.1"
                     /translation="MTDILNEDTNEGQPQNASRLSAIVAIIGRPSAGKSTFLNTACGE
                     KVSIVSPVPQTTRNAIRGIVNGSRGQLVFIDTPGYHESDKKLNLMLRSVAHDRLPESD
                     AIVYLLDACREPGSEEEAIVRLLAPAASRVCIGINKIDLPQSKAHLIRAFLSKSLPAI
                     PQDRIFEISAKNDIGLDPLLVKLFDLAPEGAALYPEEYYTDQDVDFRIAEIIREKAIL
                     NTREEIPHALYVEIADMEMKKDGKELWVRAFLTVERESQKSMVIGKGATMIKKIRLEA
                     VRDLNKIFSYRINLDLQVKVNKNWRQKDPLLGRLIKR"
     gene            complement(2132..2947)
                     /locus_tag="PKH81_03165"
     CDS             complement(2132..2947)
                     /locus_tag="PKH81_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNQ97065.1"
                     /translation="MNTLLLTLIVSLGLASLLGFLLGVFKKVFHVDVDPLVSAVREAL
                     PGANCGACGYPGCDGLAQAIAAGDAAPTACTVGGAPVASAIGALMGVSAEAETKAVVL
                     FCQGSLEHCKPKAEYVGIKTCQAAKLSVNGTKLCDWGCIGFGDCEKACPFDAIHVAED
                     GIPHVDYTKCTGCGICTGACPQKILSLVPVARRGAVALCSNRNTRKAQVIKDCKVGCI
                     KCGKCEKVCPKSCIKVTNGIPVVDYSVCDSCGECVKNCPTKVLSLVEISCAKE"
BASE COUNT          755 a          787 c          733 g          696 t
ORIGIN      
        1 tgggaaaaag gaccttaaac gggtgcgggt ttcaattatc ggcgccggcg gggctgcgcg
       61 cgcgattacc catgcgatta aacaacttgg cgccaaggcc tgtattttta accgaactac
      121 cgacaaggcc agggagctcg ccctgcgcta taactataaa taggcaggcc ttgatgctgg
      181 cagccgatcg cttttagaaa gttattccga cattattatt caaacaacca atgtgggcat
      241 gtcgcccaat atcgatgacg atcccataga tttttattcg tttaacggca acgaggccgt
      301 atacgatgta atctaccatc ccgataaaac cagaatgcta aagcgggcgg aaaaagctgg
      361 ttgccgcgtt tgtaatggat acaccatgct tgcctatcaa gcatacttgc aatatcgcct
      421 ttttaccgga gttgattatg ccgaagaatg aatgcaccct tgccgaacaa aaaacactcg
      481 ctgcccgcca tgccgttgac gaccttatca ggcgaggcga gttacgttcc aacatgaagc
      541 tcggcctggg aaccgggtct accgccatgc ctgccgttgc acgcatagcc gaacacattc
      601 gcgaaggaaa tcttaccgga atccgcgcgg ttgcaacaag ctttcaaaca acaatcgcgt
      661 gcgaagacct gggtataccc gtattctctc ttaacagcaa ggaaatctca ggagagctcg
      721 accttgctat agacggagcg gacgaaataa gtccggctaa acaccttatt aaaggcggtg
      781 gagctgcctt actcctggaa aaaatcgtag cctataacag cgctcttttt gtaatcgttg
      841 ccgactcgtc caaacgggtt actacccttg gaacgcgctt tgccctaccg gtagaagtta
      901 tcagcgaagc ccgggtcagt gttacccgtg cccttgcgtc tctgggcgca caggcactcc
      961 ttcgcgaagg agtgcgaaag gctggccctg ttgttaccga caacggcaac cttattcttg
     1021 actgtgtttg gaaagaggcg gtggacgcgt atcagatgga agaagcgatc aatcagatag
     1081 ttggtgtcgt ggaaaacgga ttttttaccc gcaccagccc cattgtctac gcagcggcca
     1141 ataccgggat tgttgagcgc gtgtagcgct caacagccta tcgtttaatc aggcgtccaa
     1201 gcaggggatc tttttgtctc cagttcttgt tcaccttaac ctgtagatca aggttgatcc
     1261 ggtatgaaaa aatcttgttc aagtcacgaa cagcttccag ccgtatcttc ttgatcatcg
     1321 tggcgccttt tccgattacc atggattttt gactttcccg ttcaacggtc aaaaaggccc
     1381 tgacccacaa ctccttaccg tccttcttca tctccatgtc ggcgatttcc acatacaggg
     1441 cgtgcggtat ctcctcgcgg gtattaagga tagccttttc gcgaataatc tccgcgatac
     1501 ggaaatccac atcctgatcg gtgtagtatt cctcgggata aagggccgcg ccctcggggg
     1561 caaggtcaaa aagtttaacc agaagaggat caagaccgat atcattcttt gccgagatct
     1621 caaagatgcg gtcctgcgga attgcgggta atgatttgga aaggaaggcc ctgataagat
     1681 gggccttgga ctggggaagg tctatcttgt taatcccgat acacacacga cttgctgccg
     1741 gcgctaataa tcgaacgata gcttcttcct cgctacccgg ttcacgacac gcgtccagca
     1801 agtacacaat cgcatctgat tcaggcagac gatcatgggc tacggagcga agcatcagat
     1861 tcagcttttt atccgattca tgatagccag gggtatcgat aaaaacgagc tgtccacggc
     1921 taccattaac gatgccccgg atagcgttgc gggttgtttg cggaaccggg gagacaattg
     1981 aaaccttctc cccgcacgca gtatttaaaa aagttgattt accggccgag ggacggccga
     2041 taatggcgac tatggcggac agtcggctcg cgttctgcgg ctgtccttcg ttggtgtctt
     2101 cgttcagaat atctgtcatt actccaggag actactcttt agcgcagctt atttcaacca
     2161 gagagagcac cttggtcgga caattcttta cgcactcacc gcatgaatcg cataccgaat
     2221 aatccacgac aggaataccg ttcgttacct tgatgcacga tttgggacat accttttcgc
     2281 atttaccgca cttaatgcag ccaaccttgc aatccttgat aacttgggcc ttgcgggtat
     2341 tacggttgga gcacagggct acagcaccgc gacgggcaac gggaacaagg gataaaatct
     2401 tttgcggaca ggcgccggta caaatgccgc agccagtaca cttagtataa tcaacgtgag
     2461 ggattccatc ctcggcaaca tgaatcgcgt caaagggaca tgctttctcg caatcgccaa
     2521 acccgataca gccccaatca cagagttttg taccgttaac cgacagcttg gcagcctgac
     2581 aggtttttat accgacgtac tcggccttgg gtttacagtg ctcaagactt ccctgacaaa
     2641 aaagcaccac agccttggtc tctgcttccg cgcttactcc catcaatgcg cctattgccg
     2701 aagcaaccgg agcgccaccg acggtacagg cagtaggagc cgcgtctcct gcggctatcg
     2761 cctgggcaag accgtcgcaa ccggggtatc cacaggcacc acaattagcg ccgggaaggg
     2821 cctcgcgaac agcggagact agaggatcaa cgtctacatg aaagaccttc ttaaagacgc
     2881 ccagcaaaaa ccccagaagg gatgcaaggc caagggaaac gatcaaggtt aataacagtg
     2941 tgttcatata tgtgcgctcc ctttatttaa t
//
LOCUS       DAOQXQ010000143         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35570_length_2971_cov_2.109739, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000143 DAOQXQ010000000
VERSION     DAOQXQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35570_length_2971_cov_2.109739"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKH81_03170"
     CDS             complement(<1..385)
                     /locus_tag="PKH81_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HNQ97066.1"
                     /translation="MSGSVRMKGRTTEEAIKSALEVLKAGREEVDVRVISEGEQAVLG
                     VFGGKDAEVEVKIKLDIAEEAKNILQDILDRAGHITQVYIQDDTAETASLEIKADDPA
                     RVIGKDGATLAAIQYLVSIISNRGKE"
     gene            complement(406..1038)
                     /locus_tag="PKH81_03175"
     CDS             complement(406..1038)
                     /locus_tag="PKH81_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNQ97067.1"
                     /translation="MVDIMLRILDLFYNIGFHNYGIAIILLTIAVKFALYPLTLQSIQ
                     QMSAMQKVQPKFDELKKKHKDKPEKFQQEMMELYKEHGINPLGGCLPILLQLPIFIAL
                     FMALTSPAFKEALAAAPQSAAFLWIPNISFKDPLYIMPVLIGISTFLSQKTMPSSASN
                     EQTKYIMLIMPFFIAFISLEFAAGVQLYWVAQNTLTVAQQMYITAKKGKV"
     gene            complement(1060..1320)
                     /gene="yidD"
                     /locus_tag="PKH81_03180"
     CDS             complement(1060..1320)
                     /gene="yidD"
                     /locus_tag="PKH81_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ97068.1"
                     /translation="MSLAEGFVRVASFVRGLLKTILIGSIKHYKLFISPAMPYSCRYY
                     PSCSQYAIEAIEKYGPLKGGAFGVARILRCNQFFPGGPDPLE"
     gene            complement(1307..1651)
                     /gene="rnpA"
                     /locus_tag="PKH81_03185"
     CDS             complement(1307..1651)
                     /gene="rnpA"
                     /locus_tag="PKH81_03185"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ97069.1"
                     /translation="MRGLFIKEQRDFNRIIKSGKQLNTPIGRLKITKNGVKTPKLGII
                     IGKTQGLTAVKRNRIRRTIKEAWRSLSVTLFEPVEIVIFPRNHIFVEKSTKIASILSR
                     AIGDAGVKDVSC"
     gene            complement(1648..1785)
                     /gene="rpmH"
                     /locus_tag="PKH81_03190"
     CDS             complement(1648..1785)
                     /gene="rpmH"
                     /locus_tag="PKH81_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ97070.1"
                     /translation="MVKRTFQPSKVHRKRVHGYRRKKASKAGRKVLSRRRKKGRRRLT
                     P"
     gene            2075..>2971
                     /gene="dnaA"
                     /locus_tag="PKH81_03195"
     CDS             2075..>2971
                     /gene="dnaA"
                     /locus_tag="PKH81_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ97071.1"
                     /translation="MTISEIDDIWRRILEDLEKGLSKPIYETILSSAKPLSIEDGVLV
                     LAVPSHFSHDWLCEKKCDESILGAIKTIGENELKSIKFVVMPKEEPSAEIKRVAPSAP
                     VYLSIPFLNPRYTFDNFVVGHGNRFAQAAALAVSESPGKAYNPLFLYGGVGLGKTHLM
                     QAIGYEVLKKIPRAKVVYVSSEKFTNELIDSIQHNTMAQFRNKYRNVDVLMIDDVQFL
                     AGKERTMEEFFHTFNTLYESSKQIILSNDRPPKEIPDIEDRLRSRFEWGLIADIQPPD
                     LETRIAILKKRAAGETVRIPDEV"
BASE COUNT          729 a          713 c          654 g          875 t
ORIGIN      
        1 gctcttttcc tctgtttgaa attatgctga ccaggtattg tatggctgcc agggtcgctc
       61 cgtccttgcc tatcaccctg gccggatcgt ctgccttgat ctccaaggat gctgtttccg
      121 cggtgtcgtc ttgaatgtat acttgcgtta tgtgccctgc cctgtccaga atgtcctgaa
      181 ggatgttctt tgcctcttca gcgatatcca gcttgatctt tacctctacc tcggcatcct
      241 tccctccaaa cactcccaga actgcctgtt cgccttctga gatcactctt acgtcaacct
      301 cttccctgcc ggccttaagc acctccaaag cgcttttaat ggcctcttcc gtggttctgc
      361 ctttcattct cacgctgccg ctcattgctc ttttcctcct ttattctata ctttcccctt
      421 tttcgcggtt atatacatct gctgggctac cgtcagggtg ttttgggcga cccagtacag
      481 ctggactccg gctgcaaact cgaggcttat gaatgcaata aaaaagggca taatcagcat
      541 tatgtacttt gtttgttcgt tagaagcgct gctgggcatc gtcttttggg atagaaatgt
      601 cgatattcct atcagtaccg gcattatata aagagggtct ttgaaggaaa tgttgggtat
      661 ccacagaaac gctgccgatt gaggcgcggc cgccagggct tccttaaatg ccgggctggt
      721 cagtgccatg aatagtgcga taaatatcgg cagctgcagc agtatcggaa ggcagcctcc
      781 aagcgggttt atgccgtgct ccttatacag ctccatcatc tcctgctgaa atttttcggg
      841 tttgtccttg tgttttttct ttaattcatc aaactttggc tgtaccttct gcattgccga
      901 catttgctgg atggattgca aagtaagagg ataaagtgca aatttaacgg ctatcgtcaa
      961 aaggattatc gcgatcccat aattgtggaa ccctatgtta tagaagagat ccagtatcct
     1021 gagcattatg tcaaccaaag ggcccattgt tgtctccttt tattctaaag ggtccgggcc
     1081 cccggggaaa aattggttac atcttaagat ccttgccacc ccaaatgcac cgccctttaa
     1141 aggtccgtac ttttcaatag cctcgatcgc atactgtgag catgagggat aatatctgca
     1201 ggaataaggc attgccggag atatgaacaa cttgtagtgc tttatgcttc ctatcaggat
     1261 tgttttaagt aacccccgaa caaaggaagc cacgcgcaca aaaccctcag caagagacat
     1321 ctttaacacc tgcatcccct atcgcccttg acaatattga agcaattttt gttgatttct
     1381 caacaaaaat atgattcctc ggaaagatca ctatttcaac cggctcaaac agagtcacag
     1441 ataggcttct ccaggcctct ttaatagttc gccttatcct gttccttttc acggcggtca
     1501 acccctgtgt ttttccaata attatgccca attttggcgt ttttacgccg tttttggtaa
     1561 ttttaagccg cccgatcggg gtatttagct gttttcctga tttgattatg cggttaaaat
     1621 ctctctgctc ttttatgaag agccctctca aggagtaagc ctgcgccttc cctttttcct
     1681 tcttctggaa agaacttttc ttccggcttt ggaggctttt tttctcctgt atccatggac
     1741 tcttttcctg tggaccttgc taggctgaaa tgttctcttt accatttatt tagtctccat
     1801 aagaaattat agataatcta tagccccttg tcaaggatgc cgattgacgt ttggataaca
     1861 aactgttaaa atcttacaac gggtgatttg ctgggcttca atgagtgcct ataatttgcc
     1921 cggcttatcc acatcttttg tttcctgtgg attaaaatgt tgataacttt gatttttgtt
     1981 gtggttttgc ctcaaatttg ttgtccttta cagtggtgcg ggcatgaata tgtagggctt
     2041 tttgaggatg catatgtgga tagattgtta aaaaatgaca attagcgaaa tagatgatat
     2101 ttggcgaaga atacttgagg atcttgagaa aggccttagc aagcccatat atgaaaccat
     2161 actgtcctct gcaaaacccc tgtcgataga agacggggtc ttggtccttg ctgtgccgag
     2221 ccacttttcg cacgactggc tttgcgaaaa gaaatgcgac gagtccatcc ttggagcgat
     2281 aaaaacaata ggggagaacg aactaaaaag catcaagttc gttgtaatgc cgaaagagga
     2341 gccttccgct gagatcaaaa gggtcgcccc gtccgcgccg gtatatttat caatcccttt
     2401 tttgaaccct agatacacgt tcgacaactt tgtagtgggg cacggcaacc gtttcgcgca
     2461 agcagccgcg ctggcggtat ccgaatcccc cggcaaggcc tacaaccctt tgttcctata
     2521 cggaggcgtg gggcttggga aaactcatct gatgcaagcc atcggatatg aggttttgaa
     2581 aaaaataccc agggcaaaag tggtttatgt ttcttcggaa aaattcacaa atgagctcat
     2641 tgattcgatc cagcacaata cgatggctca attcaggaac aaatacagga atgtcgatgt
     2701 tcttatgatc gacgatgtgc agtttttggc cggcaaagaa aggaccatgg aagagttttt
     2761 ccatacattc aacacgcttt atgaaagctc taaacagatc atcctgagca acgaccgccc
     2821 cccgaaagag atccccgata tcgaagaccg gctcaggtcc aggtttgaat gggggcttat
     2881 agccgacata cagcctcccg acctcgaaac aaggattgca attctaaaaa aacgcgcagc
     2941 aggagaaacc gtgcggatcc cggacgaggt c
//
LOCUS       DAOQXQ010000144         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35696_length_2962_cov_3.077399, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000144 DAOQXQ010000000
VERSION     DAOQXQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35696_length_2962_cov_3.077399"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKH81_03200"
     CDS             <1..989
                     /locus_tag="PKH81_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ97072.1"
                     /translation="IIMNTLPVCRERGISYATFTLVDIEPNSSVRIMEYDNPDYVLVR
                     QNVVVEPIKQAMPIERKSAKSAPTREAILYYSDYRARPGDRLVFFSDGVTQSGMGMRL
                     TPLGWGAAPVQEYILEEIAKRQDVSARELARSIVTRAVQNDQYQARDDITCAVIYFRH
                     PRDLLIMTGPPVNPDHDAEMAREFTGFNGKKILCGGTTANIVSRELGKPLKLIMKDLD
                     PVVPPMSTMDGADLVTEGIITMGMVSEILDRSSIPGGNLLVDSDRKNAATRMVELLLD
                     SDRITFLVGTKINEAHQDPNMPVELEIRRNVVKKIQSLLSERYLKEVSVRYI"
     gene            1006..1263
                     /locus_tag="PKH81_03205"
     CDS             1006..1263
                     /locus_tag="PKH81_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ97073.1"
                     /translation="MSEKIKVVICSGTACFVMGASDILLLEESLSPELRSRVEIEGAT
                     CLGMCRESERGKAPYVTINGELMSQATLPKVLARIQELVDA"
     gene            1256..2704
                     /locus_tag="PKH81_03210"
     CDS             1256..2704
                     /locus_tag="PKH81_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HNQ97074.1"
                     /translation="MLNINNGASKLKREILVRLAKLQLEGNLEDGVHYIPREMAPRNA
                     DPFRCCVYHDREVLRMRVIARLGHSVEDYDDEKKLAEYAREALAREKPTEPMLTVLDE
                     ACNACVRAHYMVTNACQACVARPCMMNCPKKCIEITGGRAHIDPEACVNCGLCMQNCP
                     YHAIIKIPVPCEEACPVGAISKDENGKERIDYDKCTFCGACMRECPFGAMMDKSQLID
                     VIKKLMDGKNVVAMYAPAIAAQFRAQGGQLEAAFAKAGFAKTYEVAVGADITADKEAH
                     EFEERMDRGDVLMTTSCCPAYVRAVHIHVPELNSCVSDTRSPMHYTAALARQNDPDAV
                     TVFIGPCLAKRKEGIDDPNVDYVLSAEDVGALFVAKGIDIGECEPVVLDTIPTTSGRN
                     FARSGGVAEAVRVRVKNPDRLRPAVINGLDAAGMKQLALYGKAAAEGKPCEANLVEVM
                     ACHGGCIAGPSVITNAKVAAIQLEKYAATGKK"
     gene            complement(2728..>2962)
                     /locus_tag="PKH81_03215"
     CDS             complement(2728..>2962)
                     /locus_tag="PKH81_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HNQ97075.1"
                     /translation="LKESGLVEHEHYGDIKLTEKGLLLAKAVRARHDALKTFLSQILG
                     VSADVAEQDACRMEHAISKETALKLEEYLKKVL"
BASE COUNT          780 a          602 c          825 g          755 t
ORIGIN      
        1 caattattat gaatacattg cctgtttgta gagaacgagg tattagctac gcaaccttta
       61 cgttggtgga catagaacca aattcttcag taaggatcat ggaatatgat aatcctgatt
      121 atgttttggt tcgacaaaac gtggtggtag aaccaattaa gcaggcgatg ccaattgagc
      181 gaaaaagcgc aaagagtgcg ccaacacggg aagcgatatt gtattactcc gattatcggg
      241 cccgtccggg agatcggctt gtattttttt ctgatggggt aacacaatca ggcatgggga
      301 tgaggcttac tcctttgggg tggggagcag ccccggttca ggaatatatt ctagaggaaa
      361 tcgccaagag acaggatgta agcgctcggg agttggcaag gtcaattgtt accagggctg
      421 tgcaaaatga tcagtatcag gcaagggatg atattacctg cgctgtcatt tatttcaggc
      481 atcctcggga tttactaatt atgacgggtc ctccggttaa tccggatcat gatgccgaga
      541 tggctcggga atttaccggt tttaacggga agaaaatcct gtgtggaggg actaccgcga
      601 atattgtttc gcgtgaactt ggaaagccgc tcaaattgat aatgaaggat cttgaccctg
      661 tggtccctcc catgtccacg atggacgggg cagatttggt tacagaggga atcataacta
      721 tgggtatggt ttcagaaatt cttgacagga gctcaattcc aggcgggaat ctgcttgttg
      781 acagtgatcg aaaaaatgca gcgactcgga tggtggaatt attactggat agtgaccgca
      841 ttaccttttt ggttggtacc aagattaacg aagcacatca agatcctaat atgcctgtgg
      901 aattagagat acggcggaac gtggttaaaa agatacaaag tctgttgagt gaacggtacc
      961 ttaaagaggt ttccgtcaga tatatataag cgtaaggagt gttgtatgtc cgaaaaaatt
     1021 aaggttgtta tctgttcagg gaccgcatgt tttgttatgg gggcctcgga catattattg
     1081 ttggaagaaa gcttgtctcc tgagcttcgg tcccgggtgg agattgaagg cgccacctgt
     1141 ttggggatgt gccgggagag cgaacggggt aaggcaccct acgttacgat aaacggagaa
     1201 ttgatgagcc aggcaacgtt gcccaaggtc ctggccagaa tacaggagtt ggtagatgct
     1261 taatataaat aacggagcct ctaagttaaa gagggaaata ttagttcgcc tggccaagct
     1321 gcagcttgaa ggaaaccttg aggacggggt tcactatatc ccacgcgaga tggcacccag
     1381 aaatgcagat cccttccgtt gttgtgtgta tcacgatcgg gaagtgctgc gcatgcgggt
     1441 aatagcccgt cttggtcaca gcgttgaaga ctatgatgat gaaaagaaat tggcagagta
     1501 tgcccgcgag gctcttgccc gagagaagcc cacggagccc atgttaaccg ttttggatga
     1561 ggcctgtaat gcttgtgtac gcgcccacta catggtcacc aatgcttgtc aggcatgtgt
     1621 ggctcgaccc tgcatgatga actgccccaa gaagtgtatc gagattaccg gcgggcgtgc
     1681 gcatattgat ccagaggctt gcgttaactg tggcttgtgc atgcaaaatt gtccgtatca
     1741 cgcaataatc aaaataccgg taccttgcga agaagcctgc cctgtgggag ccattagtaa
     1801 ggacgagaac gggaaagaac gtatagatta cgacaagtgt accttttgtg gcgcttgcat
     1861 gcgtgagtgt ccctttggcg cgatgatgga caagagtcag ttaattgatg tgataaaaaa
     1921 attgatggac gggaagaatg tagtggccat gtatgctcca gcgattgcag ctcaattcag
     1981 ggcccaaggg gggcagcttg aagcggcttt cgccaaagca ggattcgcca aaacatacga
     2041 agttgctgtg ggagcggaca ttaccgccga taaggaagct catgaatttg aggagcgaat
     2101 ggacaggggg gatgttttga tgacaacatc ttgctgccct gcttatgtac gtgcggtaca
     2161 tatccacgtt ccggaactta acagttgtgt gtcggacact cgctctccta tgcattatac
     2221 ggcggccctt gctcggcaaa atgatccaga tgcggttaca gtgtttatcg ggccttgtct
     2281 tgccaagcgc aaagaaggaa ttgatgatcc gaatgttgat tatgtgcttt ctgcagagga
     2341 tgtaggcgct ctctttgtgg ctaagggcat cgacattgga gaatgtgaac ccgttgtttt
     2401 ggatacaatc cccacaacct ccggacgtaa ctttgcacgt tcgggcggag tcgcggaagc
     2461 tgttcgagtg cgagtaaaaa atcctgatag gcttcgccct gctgtaatca atggacttga
     2521 tgcagccggc atgaaacaac ttgccttgta tggaaaggcc gcagcagaag gaaaaccctg
     2581 tgaggcaaat cttgttgagg taatggcctg tcatggagga tgtattgcag ggccgtcagt
     2641 gattaccaat gccaaggttg cagctattca gcttgagaaa tacgcagcta ccggaaaaaa
     2701 ataaagacag ggggaggaat gccggggtta taacaccttt ttaaggtatt cctccaactt
     2761 gagagcggtt tccttgctta tagcatgttc cattctacag gcatcctgtt cagcaacgtc
     2821 cgcagagacc ccaagtatct gagagagaaa tgttttaagc gcatcgtgtc gggcgcgaac
     2881 cgcttttgct agtagcaggc ctttttccgt taatttgata tcgccgtagt gttcatgttc
     2941 aactaacccg gattctttaa ga
//
LOCUS       DAOQXQ010000145         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35750_length_2959_cov_2.490702, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000145 DAOQXQ010000000
VERSION     DAOQXQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35750_length_2959_cov_2.490702"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1590
                     /locus_tag="PKH81_03220"
     CDS             85..1590
                     /locus_tag="PKH81_03220"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HNQ97076.1"
                     /translation="MTDEELLAQILMFGWAGADPSSEVIAWVEKRSLGSIKVFGWNTD
                     NTVKVAESVSLLQTRAQKGRFSIPLFVATDQEGGWIRHIKGKTSETPGNLAIGASGMP
                     ADAWYSGYYISRELAALGINLNFAPTVDLYTDHDSTVIGPRSFGEDPEAAGVLGAAFM
                     SGSRAAGVLTTAKHFPGHGDTGLDSHGKLPVISISTKTLYARELVPFTRMIEAGVPAI
                     MSGHLAFPSITGTGEPATFSTLFLKDILRDKLGFTGLVITDDIMMNGATEWAGSVSRA
                     VQLAIEAGNDIVESSTTPRFSDPLWTGTLKRMKTSPAFRSRVVESAHRVLKTKIAYFK
                     GDSSVPVIPNIAEIPSRVPDPAGQAFFLSQAVRSVTLIRGTLQPIEDSPHLLIASSFP
                     SFLHAGTKRFPQASLSGINSSLRSRARRARTVIFCLSGKEDLSALESIRDLDINLIVV
                     SALSPVPLSRVPWVDTALAVYSYSPWSFTAAFASLRGDFVPQGTLPLRDLN"
     gene            1590..2417
                     /locus_tag="PKH81_03225"
     CDS             1590..2417
                     /locus_tag="PKH81_03225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ97077.1"
                     /translation="MMVIRPFCQGEDQKALDFLLPREERAIGLAATLTPDSPLRKTVV
                     CASCLEEGSSVMGLLVLLANGILFHLCDLKDSPQIRHSLQTWLTGKTVRCIIGEASFS
                     QVLQACLPAPLRVVDYELMSVCTARQATPVTQGIHIRLCDEKDAQSLLDLQEGYEREE
                     VIPPGEAYHRTACLAALRLTLSRQIVLAAFSSSVAIAKAGTNARGFNWDQIGGVYTDP
                     KWRRKGLSEALIRGLVCDRLHAGRKVALFVKPENHAARSLYTKIGFSPVSPFRIVYY"
     gene            2461..2829
                     /locus_tag="PKH81_03230"
     CDS             2461..2829
                     /locus_tag="PKH81_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97078.1"
                     /translation="MSPVLVVEDNEINAEIIMTLLEEYGIPGELAVDGIDSVEKCTDK
                     PKDYYSLILMDIHMPRMNGYEAARKIKMDLGINAPIVAQTATHTTEISIKEHGEYIID
                     YLYKPFKPDQLFAILQRFVV"
     gene            complement(2842..2952)
                     /locus_tag="PKH81_03235"
     CDS             complement(2842..2952)
                     /locus_tag="PKH81_03235"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNQ97079.1"
                     /translation="MGTTIAAVSKLEEKGFRSAVIEGATAAWRRSIELGK"
BASE COUNT          640 a          834 c          722 g          763 t
ORIGIN      
        1 gcctcttctg tctccgatcc ctctttaccc aatttttggg atacgtagga agatgagctt
       61 cttgccacac gtatcgttca ctcgatgacg gacgaagaac tgctggccca aatattgatg
      121 tttggttggg ccggcgctga cccatcctcc gaggttatcg catgggtgga aaagaggtcc
      181 ctggggagca ttaaggtttt tggatggaac actgacaata cggtcaaggt ggccgaatcg
      241 gtttccctcc ttcaaacccg cgctcaaaag ggcaggtttt ccattccgct ttttgtggca
      301 acagaccagg aaggcggttg gatcaggcac attaagggaa aaacaagcga aactccggga
      361 aatcttgcca ttggtgcaag cggtatgccc gctgacgcat ggtattccgg ctattacata
      421 tcgcgggaac ttgccgccct gggcattaat cttaatttcg cgccaacggt tgacctgtat
      481 accgatcacg attctaccgt cattggtccc cgctcctttg gggaagatcc cgaggcagct
      541 ggtgtcctgg gtgcggcctt tatgtcggga agccgtgctg cgggagtgtt aaccacggcg
      601 aaacacttcc ccggacacgg ggataccggc ctggacagtc acggtaaact cccggttatt
      661 tctatctcta caaaaaccct ctatgctcgg gagctggtgc cctttacccg tatgattgaa
      721 gcgggtgtcc ccgctattat gagcggacat cttgcctttc cctcaattac cggaaccggt
      781 gagccggcaa ccttttctac cctctttctt aaggacattc tccgcgacaa gctcggtttt
      841 accggtcttg tgattaccga cgatattatg atgaacggcg ccaccgaatg ggccggaagc
      901 gtgtccaggg cagttcaatt ggctattgag gcgggaaacg acattgtgga atcctccact
      961 acccctcgtt tctccgatcc cctgtggacc ggaaccctta aacgaatgaa aacctctccg
     1021 gcgttccgtt cccgggtagt tgagtccgcg caccgggtgc ttaaaaccaa gattgcctac
     1081 tttaaaggcg actcaagcgt tccggttatc ccgaatatcg ccgaaattcc ctcccgggtg
     1141 ccggatccag cggggcaggc cttttttttg tcccaggcgg ttcgctcggt aacccttatt
     1201 cgcggcacct tacagccaat cgaggactcc cctcaccttc ttatcgccag ttcctttcca
     1261 tcctttttac atgcaggtac caagcggttt cctcaagctt cgctaagcgg tattaactca
     1321 tccctgcgct cccgggcccg gcgtgcgcgc actgttatct tttgcctctc cggcaaggaa
     1381 gatctttccg cccttgaatc cattcgggat cttgatatta atctgattgt tgtctctgcc
     1441 ctgtcccccg tccccctctc tcgcgttccc tgggtagata cggcccttgc cgtctacagc
     1501 tattctccct ggtcctttac cgcagccttt gcttccctta ggggagattt tgttccgcaa
     1561 ggaaccctac cccttagaga ccttaactga tgatggtaat ccggcccttc tgtcaaggcg
     1621 aagatcagaa agcccttgat ttcttgcttc cgcgagaaga gcgggctatc ggtcttgccg
     1681 caacgctcac tcctgattct cccttacgca agacggtcgt ctgcgcctca tgtctggaag
     1741 agggctcttc agttatgggg cttttggtcc tgctggcaaa cggtatcctg ttccatctat
     1801 gcgaccttaa ggacagcccg cagattcgcc attcattaca aacatggctt accggcaaaa
     1861 ccgttcgctg cataatcgga gaggcttcct tttcacaggt gcttcaagcc tgtcttcctg
     1921 caccccttcg cgtcgttgac tatgagctca tgtcggtctg tacggcacgt caagcgaccc
     1981 cggtaaccca ggggatacac atccgcctct gtgacgaaaa agacgcccaa tccctgcttg
     2041 atctgcaaga aggctacgag cgggaggagg ttatcccccc gggagaggcc taccatcgaa
     2101 cggcctgcct ggccgcgctc cgcctcacct tatcccgcca gattgtcctt gccgcttttt
     2161 cttcttctgt tgccattgcc aaggccggca ctaacgcgcg cgggtttaac tgggaccaga
     2221 taggcggggt gtacaccgat ccaaaatggc gaagaaaagg actatccgaa gcccttatca
     2281 ggggcctggt atgtgacaga ctacacgctg gtcgcaaggt ggctcttttt gttaaaccag
     2341 aaaaccatgc cgcccgctcg ctctacacaa aaattggctt ttcccctgtt tctcccttta
     2401 gaatcgttta ctattgattt ttgccaaaag agcgtatact taagggcagg agtttttaag
     2461 atgtcacctg ttctggttgt tgaagataac gagataaatg cagagatcat catgactttg
     2521 cttgaagaat atggaattcc gggagaattg gcggtagatg gcattgattc ggtagaaaaa
     2581 tgcaccgaca aacccaaaga ttattactca ctcattttaa tggatattca tatgccccgg
     2641 atgaacgggt atgaggcagc cagaaaaatc aaaatggatc tgggtatcaa cgctccaatc
     2701 gtggcgcaaa cggccactca cactacagaa atttccatca aggaacacgg cgagtatatc
     2761 attgactatc tgtataagcc ctttaagccc gatcagttat tcgctattct gcagcggttt
     2821 gtcgtgtagg gcaacgctcc cttatttccc cagttctatg gatctacgcc aagcggcagt
     2881 ggcgccctct attaccgcag accggaatcc cttttcctca agcttggaca cagctgctat
     2941 tgttgtaccc atgggagag
//
LOCUS       DAOQXQ010000146         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_35782_length_2957_cov_2.854238, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000146 DAOQXQ010000000
VERSION     DAOQXQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35782_length_2957_cov_2.854238"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKH81_03240"
     CDS             complement(<1..447)
                     /locus_tag="PKH81_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ97080.1"
                     /translation="MKRLFVICMLISVALLYGAGTGEVSGARQNEVVVYAYDSFIAEW
                     GPAPQLEKLFTEKTGYTVRFVSAGDAAQVLSRAVLEKKRPVADVLLGIDNNLLPLARD
                     SGVLEPYKSPSASDIPSELVLTQDYILTSYDWGYFALIYDTHSSVPE"
     gene            complement(613..2142)
                     /gene="rny"
                     /locus_tag="PKH81_03245"
     CDS             complement(613..2142)
                     /gene="rny"
                     /locus_tag="PKH81_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNQ97081.1"
                     /translation="MNILLCIGLPVAGIVLGWTIRWLYARFQLSASEQRAERVKQDAI
                     KEAEAQKKEILLEAKEQLIRERNQQERENRERRSELQRYERRLTVKEETLDKRSESMD
                     KQEERFSEQEKALVEREKFLLDEEERYRQELERISGLTSEEAKDLIIKDLENEAKHDA
                     QALLNKIEQEAQVASEKKARDILVTTIQRIATETTGDITVATVSLPSDEMKGRIIGRE
                     GRNIRTLETLTGVDVIIDDTPEAVVISCFDPVRKEIAKVSLERLILDGRIHPARIEEI
                     VQKVTREISQKIYEEGEKVLFDLGIHNMSQEGVRSLGRLYFRTSYGQNVLYHSKEVAM
                     IAGMLAAEVGANRDLAKRSALLHDIGKGAENDSDQNHAEVGMDLARKMNEDARVINAI
                     GAHHNDVEPNCIEAVLVQIADAISAARPGARRETIDNYVKRLENLEAIAESFVGVEKA
                     YAIQAGRELRVLVNNEKIPDGDVKDLGREIAKKIENDLRYPGRIRVTLIRETRIVEYA
                     R"
     gene            complement(2303..>2957)
                     /gene="lptB"
                     /locus_tag="PKH81_03250"
     CDS             complement(2303..>2957)
                     /gene="lptB"
                     /locus_tag="PKH81_03250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ97082.1"
                     /translation="SMHTGEILGLLGPNGAGKTTSFYMIVGFYRPSQGDVFLNDQCIT
                     KLPMYKRARLGISYLPQEPSVFRKLTVEQNIWSILETRRDLTHKEKLRHLDELIEEFN
                     IERIRKQQAYTLSGGERRRTEIARSLAIEPEFLLLDEPFAGIDPIAVNDIKSMVKLLA
                     QRGIGVLITDHNVRDTLEITHRSIIISTGQIVAQGSKEEILESPIARKIYLGEEFRM"
BASE COUNT          734 a          717 c          652 g          854 t
ORIGIN      
        1 ttctggaaca gaggaatggg tatcgtatat aagggcaaaa tagccccagt catagcttgt
       61 taatatatag tcttgggtaa ggacaagctc agagggaata tctgatgcgg atggactctt
      121 gtatggttca agaacgcctg agtctcgagc gagaggaaga agattgttgt ctataccaag
      181 aagaacatcg gctacgggcc tttttttttc gagcaccgca cgggacaaca cctgggctgc
      241 atcaccggcg gagacaaaac gaacagtgta tccggttttt tctgtaaaaa gcttttccag
      301 ttgcggggct ggaccccact cggcaataaa tgaatcgtag gcgtacacga caacttcgtt
      361 ttgtcgcgcc ccggaaacct ctcccgtacc cgcaccatag aggagcgcaa cagagataag
      421 catacagata acaaataagc gtttcatacc atacccccag aggggctatg gtagcgaagc
      481 ctgactaaac ttgcaaggga attgaatcgt aaaaaaaaca ccccgtaagg aagatccttc
      541 ggggtgttca tttctgcagt agcatatgcc ctatggggca ttgagtatgc cctaccgggc
      601 attgagtatg ccctaccggg catactctac aattctggtt tcgcggatca gggttacgcg
      661 aatgcgcccc ggatacctta gatcattctc aatttttttc gcaatctccc gtcccagatc
      721 cttaacgtct ccgtcgggaa ttttctcgtt attaaccagg acacgaagtt ctcgtccagc
      781 ctgaatggca taggctttct ctacaccaac aaaactttct gcgatcgctt ccaggttttc
      841 aagtcgctta acgtagttat caatcgtttc gcggcgggcg ccagggcggg cagcagagat
      901 tgcatccgca atctgtacca gtaccgcttc gatgcagttt ggttccacat cattatgatg
      961 ggcgccaatc gcgttaataa cgcgggcatc ttcattcatt ttacgggcca aatccatacc
     1021 aacttcggca tggttctgat cggaatcgtt ttccgctccc tttccaatgt catgcaagag
     1081 ggcagaacgc tttgccaaat ccctgttcgc accaacctca gccgcgagca tccccgcgat
     1141 catggcaact tccttggaat ggtacaacac attctgtccg tagctggtac ggaagtatag
     1201 ccgtccaagg gatctaaccc cttcctgact catgttgtgt ataccaagat caaacaggac
     1261 cttttcgccc tcttcgtaaa tcttctggga tatttcgcga gtaacctttt gaacgatttc
     1321 ctcaatgcga gccggatgaa tacgtccgtc cagaataagc cgttcaagag ataccttggc
     1381 aatttccttg cgaacgggat caaagcagga gataactact gcctcggggg tatcgtcaat
     1441 aatgacgtct acaccggtca gggtttcaag ggttcggata ttgcgccctt cccggccgat
     1501 aatgcgcccc ttcatctcat cgctgggtaa acttaccgtg gcaacagtaa tgtctccggt
     1561 ggtttctgtg gcaatgcgct gaatggtggt taccaggata tcacgagcct tcttttcgga
     1621 cgcaacctgt gcttcctgtt caatcttgtt gagcagggct tgcgcgtcat gcttggcttc
     1681 gttttctaaa tccttgataa taagatcctt tgcttcttcc gaggtaagcc ccgaaatccg
     1741 ctccagttct tgtcgatatc tctcttcttc gtccaaaagg aacttttctc gttcaacaag
     1801 ggccttttcc tgttcggaaa aacgctcctc ctgcttgtcc atagactcgc ttcgtttgtc
     1861 gagggtttct tctttaacgg tgagacgtct ctcgtagcgt tgaagttcac tcctacgctc
     1921 acgattttcc cgctcctgct ggtttctttc ccgaattagc tgttcttttg cttctagaag
     1981 aatttccttc ttttgagctt ctgcttcttt tatagcatcc tgttttacac gttccgctct
     2041 ctgttcagat gcagatagtt gaaatctggc gtacagccag cgaatagtcc atccaagaac
     2101 aattccagca actgggagac cgatacataa caatatgttc attgtggtcc tccaaactag
     2161 attagttcag actagagaat agcataagtt gaattacttg tcaaataaaa gattagaagg
     2221 atgctcaagc ggtataaagg gtgatgaatc gtactaaagg ttactggagg gtaaagtaat
     2281 gtaaaaaaaa gacgtttatg cgctacattc taaattcctc acctaagtat atttttcttg
     2341 caattggaga ctcaagaatt tcttcctttg aaccttgtgc aacaatttgt cctgtgctta
     2401 tgattattga acggtgcgta atttctaatg tgtcgcgcac attgtggtct gtaatgagta
     2461 ctccgattcc tctttgcgct aaaagcttta ccatcgattt aatgtcgtta acagcaatcg
     2521 gatcaattcc tgcaaagggt tcatcaagca ggagaaattc cggttcaata gcgagcgatc
     2581 ttgctatttc tgttcttcgc ctttcgcctc cggacagagt ataggcctgc tgtttgcgta
     2641 ttctttcaat gttgaattct tcaataagct catccaaatg cctaagcttt tctttatgcg
     2701 ttaaatctct tcttgtttct aatattgacc agatattctg ctctaccgta agtttccgga
     2761 aaaccgaggg ttcctgagga agataggaaa taccaaggcg ggcgcgctta tacatgggca
     2821 gtttagttat gcactgatca tttaaaaaga catccccttg gctcggccgg taaaatccga
     2881 caatcatata aaaactggtt gttttgcctg caccatttgg gcctagaagt cctaagatct
     2941 ctcctgtatg cattgaa
//
LOCUS       DAOQXQ010000147         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36054_length_2942_cov_2.438517, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000147 DAOQXQ010000000
VERSION     DAOQXQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36054_length_2942_cov_2.438517"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PKH81_03255"
     CDS             <1..922
                     /locus_tag="PKH81_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97083.1"
                     /translation="TIAAAGGHNLIAWGSPGSGKTLALRRFPALLPLLDTERALEVTR
                     IYGLAGISAGEQGLITRAPFREPHQGASLEGMTGGGVRLKPGEVSLAHGGALFLDEAA
                     QFKGSVLQSLRGPLETGWVTVSRAGKTERYPARFQLLLTMNPCPCGNFGSMNKPCLCA
                     PEAVDRYWKRLSAPLLDRMDIRVAVTNPGQSMAGEEGENTELLRARIAVARLRQWRRN
                     RRYSHYWLNAHLQASHIDEVCKMESQAQKVYRAAVGKGDLSGRGAHAVCKVARTIADM
                     ADKDLIGEAELQEALSLHAWGEAAPIFLYA"
     gene            complement(919..1758)
                     /locus_tag="PKH81_03260"
     CDS             complement(919..1758)
                     /locus_tag="PKH81_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ97084.1"
                     /translation="MIDLHVHSTASDGSFSPEALVAKALEEGLTILALTDHDTIAGLA
                     AARQAALGSQLRLIGGIELDIEWKSGECHLLGYGIDEPSPRMIEMLERVNQGRRTRNE
                     TITMRFHEAGINLDLDRVEAIADGGTVGRPHFARALVEMGAVKNIQQAFDRYLARDRP
                     FFVDRKSLSLEEGVSIISESGGIPVLAHPLSLYVSWGKLSGILADFKAQGVRGIEAWH
                     PGVRVTDARRLEALAREHSLLVTAGSDFHGAARPERRLGHTTGKLVIEERFWSEELGP
                     ALS"
     gene            1820..2788
                     /locus_tag="PKH81_03265"
     CDS             1820..2788
                     /locus_tag="PKH81_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ97085.1"
                     /translation="MPKAVQYKPNSVIYFAGDLDEKVFLLQKGRIALTSTDIETGKQV
                     TEYIREGEFFGVKSALGHFPREESVMVITDAIVLAFSTMEFETFVQNNTRIIMKMLKV
                     FSNQLRHIHRQIESLLHSEEQTNPDDGMFSVANCFFKSQQYKAASEVAIRYLKLFPSG
                     RHVQEMKQIAQNTLTSSNSFGERTASDKPQIRQEGGPVGNDPNLALTLAEGLAGQQKW
                     DEAYKQYHSVIEVGDSPALGKAYVGAGMCLYEQEEYARCVQLLTAFIAHDPKSMQMGE
                     ILMFMGLCYQKMEQIDKAKAFFAKALTMAGPQLAPRIKELQASCGG"
     gene            2792..>2942
                     /locus_tag="PKH81_03270"
     CDS             2792..>2942
                     /locus_tag="PKH81_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ97086.1"
                     /translation="MSDVFAAFSRFAKTFPKGSVIFSEFEPGDCFYLIQTGRVQLIKI
                     VNGFEK"
BASE COUNT          771 a          789 c          726 g          656 t
ORIGIN      
        1 gaccatagcc gccgcgggag gacacaacct tattgcctgg ggttctcccg gttcagggaa
       61 aaccctggct ctacggcgtt ttcccgcact gttacccctt ctggataccg aacgcgccct
      121 ggaggtgacc cgcatttatg gccttgccgg cattagtgcc ggagagcaag gtcttattac
      181 ccgggcccct ttccgtgaac cccatcaggg agcctcccta gaagggatga cgggaggagg
      241 cgtacggctc aaacccgggg aagtttccct ggcccatggg ggggccctgt ttttagacga
      301 agcggcccaa tttaagggaa gcgtactgca atccctgcgc ggtcctttgg aaactggatg
      361 ggtaacggta agccgcgccg gtaaaacaga gcgatacccg gcccgatttc agctcttgct
      421 taccatgaat ccctgtccct gtggcaactt tggcagcatg aataaaccct gcctgtgcgc
      481 tccagaggcg gtagatcggt actggaaacg cctgagcgcc ccattgctgg atcgtatgga
      541 tatccgggta gcggtaacaa accccggaca atccatggct ggggaagaag gagaaaacac
      601 cgagctattg cgggcaagga tagccgttgc acgcctgcga caatggagac gaaatcgccg
      661 gtattcacac tactggctca acgctcacct ccaggctagt catattgatg aggtatgcaa
      721 gatggaaagc caggctcaaa aggtataccg ggccgcggtg ggaaagggag acttatctgg
      781 aaggggggcc catgccgtgt gtaaggtggc cagaacaata gcggatatgg ccgataaaga
      841 tcttattgga gaggcagaac ttcaggaagc cctgtcgtta catgcctggg gagaggcagc
      901 cccgatattt ttatacgctt aagaaagcgc cggtccaagc tcctcgctcc aaaaacgctc
      961 ttctattaca agcttgccgg ttgtatggcc aagcctgcgc tcaggccgag ctgcgccatg
     1021 aaagtcgctt cccgcggtta ctaacagaga atgctcccgg gccagggctt caagacgtcg
     1081 ggcatcggtt accctgaccc caggatgcca ggcctctatt ccacgtaccc cctgcgcctt
     1141 aaaatcggcc aatataccag aaagctttcc ccaggaaaca tacaaagaca gaggatgagc
     1201 caaaaccggt ataccgcccg actcagaaat aatcgacacc ccttcttcta acgaaagact
     1261 tttccgatcg acaaaaaaag ggcgatcgcg ggcaagatag cgatcaaaag cttgttggat
     1321 atttttaaca gctcccattt caaccagggc acgggcaaaa tgagggcgcc ctaccgtccc
     1381 cccatcagcg atagcctcta cccgatcaag atccagatta atacccgctt catggaagcg
     1441 catggtaata gtttcgtttc gagttcgtcg tccctggtta acccgttcaa gcatctctat
     1501 catccgggga gaaggttcat caataccata gcccaacagg tgacactcac ccgacttcca
     1561 ttcaatatca agctcgattc cgccaataag gcgtaactga gatcccaaag ccgcctgtcg
     1621 ggccgctgcc aacccggcaa tcgtatcatg gtctgtaagg gcaagaatcg taagcccttc
     1681 ctccaaagcc ttggctacca aagcctctgg agaaaaggat ccgtctgaag cggttgaatg
     1741 gacgtgtaga tctatcatat agatggagat agtagcaaaa aaatggacga ataaccatag
     1801 aatgtgctat actcccccta tgcccaaagc cgttcaatac aagccgaatt ccgtcatcta
     1861 cttcgcgggg gacctggacg aaaaggtttt tttgctccaa aaaggacgaa tcgcccttac
     1921 ctcaaccgat attgaaacgg gaaaacaagt taccgagtac attcgagaag gtgagttctt
     1981 cggcgtaaaa tccgccttgg gccattttcc ccgtgaagaa agcgttatgg ttatcaccga
     2041 tgccatagta ctcgccttct ctactatgga atttgaaacc tttgtccaaa acaacacccg
     2101 cattatcatg aagatgctca aggttttttc caaccaactc aggcatattc accgccaaat
     2161 tgagtctttg cttcatagcg aagagcaaac caatcccgat gacggaatgt tttcggtggc
     2221 taattgtttt tttaaatctc agcaatacaa agctgcatcc gaggtagcca ttcgctacct
     2281 taaactcttc ccctccggcc gccatgtgca ggagatgaag caaattgccc aaaataccct
     2341 tacctcttcc aattcttttg gagagcgaac cgccagcgat aaaccccaaa ttagacaaga
     2401 gggcggcccg gtgggtaacg atcccaatct tgcccttact ctggccgaag ggcttgcagg
     2461 gcaacaaaaa tgggacgaag catacaaaca gtaccattcg gttatcgagg taggagattc
     2521 ccccgctctg ggaaaggcct atgtaggagc cggtatgtgt ttatacgagc aagaagaata
     2581 tgcacgctgt gtacaattac taacggcctt tatcgcccat gatcccaaat ccatgcaaat
     2641 gggagaaatc cttatgttta tgggactgtg ttaccaaaaa atggaacaaa tcgacaaggc
     2701 gaaggccttt tttgccaagg cccttaccat ggccggcccc caactcgctc ctcgtatcaa
     2761 ggagcttcag gcatcgtgtg gaggttgacc tgtgagtgac gtatttgccg ctttttcgcg
     2821 gtttgccaaa acttttccca agggatcggt tatcttttcc gagtttgaac ccggcgattg
     2881 tttttacctg attcaaaccg gtcgagtcca gcttatcaag atcgtaaacg gctttgagaa
     2941 aa
//
LOCUS       DAOQXQ010000148         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36260_length_2932_cov_2.958637, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000148 DAOQXQ010000000
VERSION     DAOQXQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36260_length_2932_cov_2.958637"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKH81_03275"
     CDS             <1..424
                     /locus_tag="PKH81_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97087.1"
                     /translation="LMGDGAIVIWDDTNEQSVGEALSVFDTYVDFVNEELFKPFDGLG
                     LAGALVTDKVFKYEISAETSQLKYRDYVGYGINLACRLQGLAKKDELILNHNLARTGL
                     VPYITQSTQDIAHVLHHLKGLKEEDCQDLLYYQPERTR"
     gene            complement(400..1134)
                     /locus_tag="PKH81_03280"
     CDS             complement(400..1134)
                     /locus_tag="PKH81_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNQ97088.1"
                     /translation="MKQGLQAETIRRIERNLSLLLDKSPTVSRQLLPMLTPASLKKAY
                     YLKARALHPDTADQRECQTQERTRLFIEVKDAYDELLIAWNAGEFESLLSTNQRATHK
                     QSEMPGKTRIFHRGSLPSMELRFGQFLYYSGTIDWHTLIDSVSWQLRERPKIGDLAIE
                     TGYLDAWDVMAIICKKGSGELFGDAALRLGYLTAYERIVLLGRQRLLGMPIGSYYIRN
                     GHIGEQEIEGQLFSQRRHNYRVRSGW"
     gene            complement(1131..1664)
                     /locus_tag="PKH81_03285"
     CDS             complement(1131..1664)
                     /locus_tag="PKH81_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ97089.1"
                     /translation="MNVFAVSGIVSAIVGLIMLLSPEGFISALVVSLGVASFASGVFT
                     LLQFRKAVDEQAFRISLTIRAFAGMAVGITAIFLPIVVAGTVWIALSYLVASYLTISA
                     LVGFYGTYRLKVAQVPYLPYLYESVISLAAALILFIFPGTTGIVLVRIGGAMITLLGV
                     LLFLKGFGILGKKTGSQ"
     gene            1794..2822
                     /locus_tag="PKH81_03290"
     CDS             1794..2822
                     /locus_tag="PKH81_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ97090.1"
                     /translation="MNRTLRSRRSGRLVSILALTLLCVGFVSCRAEKSKSEASSKERQ
                     LIVGFSQIGAESAWRKCNTRSIRDAAETYGIQLLFDNAEQKQENQIKAIRSFIAYRVD
                     VIAFVPIVSDGWENVLREARDAGIPVLITDRKIRISEEGLHAGFIGTDSFEEGRNAGR
                     FLVRKFLEKNPTRQEPAKQVRVFELTGTIGSSVATGRAMGFREVIANHPEIKIVHSES
                     GDFLRSKGYEIAKRCISDLLVVDAIYSHNDSMTLGVIDALKEAGIAPGKDVVIITIDA
                     EQASIDALRRGEVNCVIECNPKTGPAIMDLAVRLAEGESIPWIVHVDEQVFTEFDDDL
                     DAIESRGY"
BASE COUNT          786 a          647 c          767 g          732 t
ORIGIN      
        1 attgatgggt gatggcgcca ttgttatttg ggatgatacc aacgaacaaa gtgtgggcga
       61 agctctttct gttttcgaca cctatgtgga ttttgttaat gaggaacttt ttaaaccctt
      121 tgacgggctc ggtcttgcag gcgccctggt aacggataag gtgttcaagt acgaaatttc
      181 tgccgaaacg tctcagttaa aataccgcga ttatgtaggg tatggaataa atcttgcgtg
      241 ccggcttcag gggcttgcca agaaggatga gctgatttta aaccataacc ttgcacgaac
      301 aggactggtt ccctatataa ctcaaagtac ccaggacatt gcccatgtat tacatcatct
      361 aaaggggctt aaagaagagg attgtcagga cctgctgtat taccagccgg agcgaacccg
      421 gtaattgtgt cttctttgcg aaaacagctg tccctctatc tcctgttcac ctatgtgtcc
      481 gttacgaata taataacttc cgataggcat acccaataat cgctgacgcc ctaataaaac
      541 gatgcgctcg taggcggtaa ggtatccaag ccggagagca gcgtctccaa agagttctcc
      601 acttcccttt ttacagataa tggccatcac atcccaggca tcaaggtatc ccgtttcgat
      661 ggcaaggtcg ccaatcttgg gtctttcgcg taactgccag gaaaccgagt caatcagcgt
      721 atgccagtcg atggtacccg agtaatacaa gaattgaccg aaacgtaatt ccatggaagg
      781 aagactgcct cgatgaaaaa tcctggtttt accaggcatc tcgctttgtt tatgcgtggc
      841 ccgctggttg gttgacaaca gggactcaaa ctcaccggca ttccatgcga tcagaagctc
      901 atcgtaggca tcttttactt caataaagag tcgggttcgc tcctgcgttt ggcactcccg
      961 ttgatcggca gtgtcaggat gcagggcgcg agcctttaga taataggcct ttttcagact
     1021 ggcaggagta agcattggta agagctgccg gcttaccgtt ggactcttgt caaggaggag
     1081 gctcagattt cgctctatac gccgaatggt ttcagcttgt aggccctgct tcattggctg
     1141 cctgttttct ttccaaggat tccaaatccc tttaaaaaca gcaaaacccc aagaagggtg
     1201 atcattgcgc cgcctatacg aacaagaacg attccggttg tgccgggaaa gataaaaaga
     1261 ataagggctg cggcaaggga aatgaccgat tcatagagat agggcaggta gggtacctgg
     1321 gctaccttga ggcgataggt gccatagaag cccaccaggg ccgaaatggt taaatatgag
     1381 gctacgaggt acgaaagggc aatccagacg gtgccggcaa caacaatggg caagaatatg
     1441 gcggtaatac ctacggccat accggcaaag gctcgtatgg tgagcgatat gcgaaaggct
     1501 tgttcatcca ccgcttttct gaattgaagc agggtaaaga caccgcttgc aaacgaggcg
     1561 actccaagag acactacaag agcactgata aagccctcgg gcgacaagag cataataagc
     1621 ccgacaatgg cactgactat tccggataca gcaaatacat tcatagttgt tcccccataa
     1681 cgtaagtata ccacggttgt ggctttactg ggcgctttgc ccacacgtaa agtccttgcg
     1741 tgacaggaaa taatgggcct ataatgagtg tacctacacg caaaggaggg gagatgaacc
     1801 gtactctaag atcccgtaga tccggacggc ttgtgtcaat ccttgcactc accttactct
     1861 gtgtcggctt tgtgtcctgc cgggcggaaa agagtaagtc ggaagcgtca tcaaaggagc
     1921 gtcagctcat tgtgggtttt tcccagattg gggcggaaag tgcctggaga aaatgcaata
     1981 cgcgatcgat tagggacgca gcagaaacct acgggattca actgctgttt gataacgcag
     2041 agcaaaaaca agaaaaccag attaaggcaa ttcgttcgtt tattgcgtat agggtagatg
     2101 ttatagcctt tgtgcccatc gtctccgatg ggtgggagaa tgtattacgc gaggcacgcg
     2161 atgccggtat tcctgtattg attaccgatc gtaaaatcag aattagcgag gaaggtttgc
     2221 atgccgggtt tatcgggaca gacagttttg aagaaggtag aaacgcgggc aggtttctgg
     2281 tccgtaaatt tctggaaaaa aacccgactc gtcaagaacc agcaaaacag gttcgcgtct
     2341 ttgaattgac gggtactatc ggttcatcgg ttgcaacggg tagggcgatg ggctttaggg
     2401 aagtaatcgc aaaccatccg gaaatcaaga ttgtccattc cgaaagcgga gatttcttgc
     2461 gttcaaaggg atatgaaatt gccaagagat gtatctccga tttactggta gtagacgcca
     2521 tttattccca taacgacagc atgacccttg gggtcattga tgcgcttaag gaagcaggca
     2581 ttgcacctgg caaggatgtg gtgattatta ccattgacgc ggaacaggct tctatcgatg
     2641 ccctgcgtcg tggcgaggtc aattgcgtaa ttgaatgtaa tcctaaaacc ggtccggcca
     2701 ttatggattt agcggtacgc cttgcggagg gagaaagtat tccctggatt gttcatgtgg
     2761 atgaacaggt gtttactgaa tttgatgatg atctggatgc tatcgagagc cgggggtatt
     2821 gatgttcaag tttccccgta tgcgcagtat tgagcggaag cttaaaacct cctacatggt
     2881 cattatcgcg gtaatgaaac ttattcccgt tatgacaatg gcaagttctc tt
//
LOCUS       DAOQXQ010000149         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36312_length_2930_cov_2.777739, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000149 DAOQXQ010000000
VERSION     DAOQXQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36312_length_2930_cov_2.777739"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKH81_03295"
     CDS             <1..199
                     /locus_tag="PKH81_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97091.1"
                     /translation="AASSTSATPRRVVAVKRGDGTPSSAPGSRSAPPDRRPAPSRQPR
                     NEREDDGMTYNPFADLLKGKR"
     gene            202..927
                     /gene="rsmG"
                     /locus_tag="PKH81_03300"
     CDS             202..927
                     /gene="rsmG"
                     /locus_tag="PKH81_03300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNQ97092.1"
                     /translation="MAKTVGAVPSFPLSGRALLQSGLEKIGLAGEDVLAGEETPARRG
                     ATSLIPLLEQYMGELELFNAVFDLVGADTRDDLIVRHILDSLAPWKAVAALLARKAGS
                     TLADAGTGAGFPGIPLALLFPQTPVFLLERMSKRCAFLNGLRAVLRLSTVSVLEGEIE
                     KASGPYDVVVFRAFRPLEPPMFTALSRLVCPGGSLAAWKARRDKIEAEMAPLGREGVL
                     WESKPVQVPFLDHEERHLVIIPR"
     gene            complement(947..2311)
                     /locus_tag="PKH81_03305"
     CDS             complement(947..2311)
                     /locus_tag="PKH81_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97093.1"
                     /translation="MNVSITLVVVIIVAALSILLLSFLGKKKETKAPKKKKHKDRASI
                     IREATKRLSQNPRDPEGLQAMGSLHYQEQEWEKAYAAYEILIDMAPNHAKLDEFECAL
                     RMGVSAVKTNRLQEALKGFLMARKLKSDHFEVNYNLGYICFLQKDYEKAAMFLKQALL
                     ADTESVLAQRYMGFSLHKAHRYREALGYLKKAVDLQPDDKEALFAMAECFYESGASER
                     AQKIFSHLRPDPTLGPQSALYAGIIHSQTNQADKAIVDFEIGLKHETIPPEIANELRY
                     KMAVTLIKIQDISRALLTLKDIQRITPGYKDVPALIVRYQELNQNKNLQVYLLAVQSE
                     FINLCRKIVIQFYPGAKVKITDISVFADYSDVIAEIDTPKWSDIVIFRFFRSQGVVGE
                     LLLRDLYGRIKDLKAGKGICMSAGIFSEESRRFTEGRPIDLYDKDRLNKLLNAVDSGV
                     SLKA"
     gene            2421..>2930
                     /locus_tag="PKH81_03310"
     CDS             2421..>2930
                     /locus_tag="PKH81_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNQ97094.1"
                     /translation="MGAGSIGIKLADSKFFPVLEEGNPASKILELTTVRDEQTSVQIN
                     LFRSEDGELDESAYVGTLIIEDISPRASGEPTVELTLTLDEQNELSAEAVDLDSGSRQ
                     ALSVSLETLSREDLYSLPDFDLSPIDSSLHLGEDPDHHNLAYQGTIPGNAPEGLYEMT
                     NEEEKKSGVF"
BASE COUNT          633 a          711 c          803 g          783 t
ORIGIN      
        1 ggctgcctct tccacttccg ctacaccccg ccgcgttgtg gcggtaaagc gtggcgacgg
       61 cactccgtct tctgcacctg gttctcgctc tgcaccgccc gatcgccggc ctgctccttc
      121 ccggcaacca agaaatgaac gcgaagacga cggtatgacc tacaatccct ttgcagacct
      181 tttaaagggt aagcgctagt cgtggcaaaa acggttggcg ctgttccgtc gtttccgctt
      241 tccggaagag ccttgctgca gtcaggcctt gaaaagatag gtctggccgg cgaagacgtc
      301 ctggcgggcg aggaaacccc cgcgcgccgt ggagccacct cgctcattcc ccttcttgaa
      361 cagtacatgg gtgaactgga gctatttaac gcggtgtttg acctggtggg ggcggatacc
      421 cgtgacgact tgattgttcg ccatattctg gacagtcttg ccccctggaa ggcggttgcc
      481 gctctgcttg cccgcaaggc cggatcaact ctggccgatg ccggcacggg agccgggttc
      541 ccgggtattc ctcttgctct gttgtttccc cagactccgg tatttcttct tgagcgaatg
      601 agtaaacgat gcgctttttt gaacggtctt cgtgcggtac tcaggctttc tactgtttcg
      661 gtacttgaag gcgagattga gaaggcaagt gggccctatg atgtggtggt attccgggct
      721 tttaggcccc ttgaaccgcc aatgtttacg gccctttccc gacttgtctg tcctggaggc
      781 tccctggccg cctggaaggc acgacgggac aagatagagg ccgagatggc tccgcttggc
      841 agggaagggg tactctggga gtcaaaacca gtgcaggtgc cgtttttaga ccatgaagag
      901 cgtcatctgg tgattattcc ccgataaggg gcaagtcctt acaggatcag gctttaaggg
      961 acactcctga atctaccgcg ttaagcagtt tgttcaggcg gtccttgtcg tagaggtcta
     1021 tggggcgtcc ttcggtaaag cgccttgatt cttccgaaaa aattcctgcg gacatacaga
     1081 tgccttttcc ggctttcagg tctttaatac gtccatagag gtcgcgcaac aacaattctc
     1141 ctaccactcc ctgagaacgg aaaaacctga atataacgat gtccgaccat tttggggtgt
     1201 cgatttcggc gatgacgtcg gaatagtcgg caaacaccga gatgtcggta atttttacct
     1261 ttgcgcccgg ataaaactgt atgacgatct ttcggcacag gttgataaac tcgctttgga
     1321 cggcaagcag atacacttgc aggttcttgt tctggttcag ttcttgatag cggactatga
     1381 gcgcgggtac gtccttatag ccaggggtaa tccgctgaat gtctttaagg gttaagaggg
     1441 ctcgcgagat atcctgtatc tttatcaggg taaccgccat tttgtaccgc agttcgttgg
     1501 ctatttcggg tggaatggtt tcgtgtttta atcctatttc aaagtcaaca atggccttgt
     1561 ccgcctggtt ggtttggctg tgaatgatgc cggcatagag ggctgattgt ggtccaaggg
     1621 tggggtcggg acgaaggtga gagaagattt tttgggctcg ctcggaggct cctgattcgt
     1681 aaaaacattc tgccatggca aaaagggcct ctttgtcgtc cggctgaagg tctactgcct
     1741 tttttagata gccaagggct tcccggtagc gatgcgcctt gtggaggcta aatcccatgt
     1801 accgttgggc caagacgctt tcggtgtcgg ccaggagggc ttgtttaagg aacatagcgg
     1861 ctttttcgta gtctttttgt aaaaaacaga tgtagccaag gttataatta acctcaaaat
     1921 gatcgctttt gagttttcgg gccattaaaa agcccttaag cgcttcctga aggcggtttg
     1981 ttttgacggc tgagacgccc attctgagcg cgcattcaaa ttcgtccaat tttgcgtggt
     2041 ttggggccat atcgataagg atttcgtagg cggcataggc cttttcccat tcctgttcct
     2101 gataatgaag ggaacccatg gcctgaaggc cttccggatc acgcggattt tgagacagtc
     2161 gtttggtcgc ttcacgaatt attgaagctc tatccttgtg ttttttcttt tttggggcct
     2221 tggtttcttt ttttttgcca agaaaggaca gcaaaagaat ggagagggcc gctacgatga
     2281 tgacaacgac gagcgtgatg ctaacattca tatcattaca ttatgctccc agtggctgga
     2341 gctgtcaaca tttcacgttg acaagactaa tcgatgcgta ctacagtaaa ttcaagagtt
     2401 ttctcaagtg aggtatgcgt atgggagccg gttctatcgg catcaaactg gcggacagca
     2461 agtttttccc ggtacttgaa gagggaaatc cggccagcaa gattcttgaa ctcaccacgg
     2521 tgcgcgacga gcaaaccagc gtccagataa atctatttcg cagcgaagac ggtgagcttg
     2581 atgagtctgc ctatgttggc acgcttatta tcgaagatat ctccccgcgt gcgtcgggcg
     2641 agcccacggt agagttaacg cttactctgg acgagcaaaa cgagctttct gccgaggcgg
     2701 tagatcttga ttcggggtcc cgccaagccc tgtctgtttc gttggaaacc ctttcccgcg
     2761 aggatttgta ttcgcttccc gattttgatt taagcccaat agattcttcc cttcacttgg
     2821 gggaggatcc cgatcatcat aacctggcgt accagggaac gattcccggc aatgcgccgg
     2881 aaggattata tgaaatgaca aacgaagaag aaaaaaagag cggcgtgttt
//
LOCUS       DAOQXQ010000150         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36525_length_2918_cov_3.590988, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000150 DAOQXQ010000000
VERSION     DAOQXQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36525_length_2918_cov_3.590988"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKH81_03315"
     CDS             complement(<1..711)
                     /locus_tag="PKH81_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97095.1"
                     /translation="MKQGLYAIVVLLFLSFSACSSRSGDKDQTAADNSATPATETTRS
                     GWYYLSEQGIHSVSSPSVIPAREFTPWTESPRVSDMAVINGRPSFLINRIGVMSGSAN
                     GLQSALHAHPEQFSSVTAAGFIQTNGKTGIRVYTNSFFAAPQGVGPQDVFLLYYAPVS
                     GMVTPWLTVQEMGLDPLNQCVALDQVGSMWYASFKKESNEKITFTYLEFDSFPLAATS
                     RKIDADSYRRAVSPFPFSG"
     gene            complement(738..1559)
                     /locus_tag="PKH81_03320"
     CDS             complement(738..1559)
                     /locus_tag="PKH81_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97096.1"
                     /translation="MNLYLSGSVILLSTFLCACSPIASLMDSTERVHFSCASSSTLGE
                     YSGSGIQQSWTVTWYDSAGSLQRRSGVIAETDIELEKGHFTPILAQREVSDNSVVMAE
                     VLPAIGCLYPHQGRHEKGGIFITANELGGIVASVAFKAIKDAGYSRGHQIAAHCNWDK
                     CIERVASLKEPGLLDRDRFVLALLGGKVRSYDIVGQKKKAIRIEDRRSYIPRGEPFIP
                     AWYGSEAITIEEGASFSLALPSGTSYLFSPSGFLSVTIREEGPPVSFFTPYRLQD"
     gene            1665..2693
                     /locus_tag="PKH81_03325"
     CDS             1665..2693
                     /locus_tag="PKH81_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ97097.1"
                     /translation="MKALFIGGTGTISQGVAVRAREAGWDVWLLNRGNRQDRVVSGVT
                     SLKGDINADEAGVAALIKEMHFDVVVNFIAFVPEQLERDVRLFTGKTGQFIFISSASA
                     YQKPLNHYRITESTPLVNPVWEYSRNKIACEEFLMDQYRTKGFPVTIVRPSHTYDDRY
                     LPVAIHGKKGSWQVVDRILKDKPVLVHGDGTSLWTLTHSSDFAKAFVALLGNPLALGE
                     TFHITSDESLSWNQIYDSLGSALGKTVHKYHVATDFLAACDPEYSGTLLGDKAHTVVF
                     DNTKIKRILPDFTTSVSFAEGARRCVRYLLDNEQSQIPDPDFDAFCERIIQVQEAACS
                     AYFASITR"
     gene            complement(2706..>2918)
                     /locus_tag="PKH81_03330"
     CDS             complement(2706..>2918)
                     /locus_tag="PKH81_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97098.1"
                     /translation="CDIAIVPLGQKHTMRSIDDAVESVLDTKASIGIPTHYGFAEGTD
                     SDAISFVEKLAKRGSRGFIAPPLKKK"
BASE COUNT          824 a          688 c          724 g          682 t
ORIGIN      
        1 gccggagaag gggaaagggg ataccgcacg gcgataggaa tctgcgtcaa tcttacgtga
       61 ggtggcggct agcgggaagg aatcaaattc aagataggta aacgtaatct tctcattcga
      121 ttctttcttg aacgaggcat accacataga acccacctgg tccagcgcga cgcattgatt
      181 gagcggatca agacccatct cttgcacggt aagccagggc gttaccatgc ccgaaacagg
      241 agcataatag agcaagaata catcttgtgg ccctacaccc tgaggagcgg caaaaaagga
      301 attggtatac acacgaatcc ccgttttacc gttggtttga ataaaccctg cggctgtgac
      361 cgaagaaaac tgttcagggt gggcatgaag ggcactttga aggccattgg ctgagccgct
      421 catcacaccg atgcggttaa tcaaaaagct ggggcggccg ttgataaccg ccatatctga
      481 aacgcgggga gactctgtcc agggagtaaa ctccctggcg ggaataacgg agggagaact
      541 gacggaatga atcccctgtt cactcaggta ataccaaccc gagcgggttg tttcagttgc
      601 aggggtagca gaattatctg cggcagtttg atctttatcg cccgatcgtg aggaacacgc
      661 ggaaaacgac aaaaaaagga gcactacaat ggcgtacaaa ccttgcttca taataggatt
      721 acagtatccg aggaaagtta atcttgcaag cgatacgggg tgaaaaagga caccggagga
      781 ccttcctccc gtatcgtaac ggatagaaaa ccggaaggag agaataaata actagtaccg
      841 gaggggaggg ctaaggaaaa agacgctcct tcttcaatag taatcgcttc gcttccgtac
      901 caagcgggga taaacggctc tcctcgggga atataggatc gccgatcctc gatacgaatc
      961 gccttctttt tttggccgac aatatcataa gagcgaacct ttcctccaag gagggcgagt
     1021 acaaaacgat ccctgtccag aagacccggc tccttaagcg aagcaactcg ttcgatacat
     1081 ttatcccagt tacaatgagc ggcaatctgg tgccctctac tataaccggc gtcttttata
     1141 gccttgaaag ccacagaagc gacaatgccg ccaagctcgt tcgccgtaat aaagatacca
     1201 cccttttcat gccgcccttg atgggggtat agacacccaa tggccggtaa cacctccgcc
     1261 ataacaacag agttatctga aacttctctt tgcgcaagaa tgggcgtgaa gtggcccttt
     1321 tccagttcaa tatccgtctc tgcgataacg ccagagcgtc tttgtagaga acccgctgag
     1381 tcgtaccatg taaccgtcca gctttgttga atacctgaac cggaatactc gcctaacgta
     1441 gaagacgagg cacaagagaa gtgcactcgc tccgttgaat ccatcaggga tgcaatggga
     1501 gagcaggcac ataaaaaggt tgaaagaaga attactgatc cagagagata aagattcata
     1561 cctcttcttg gtcctaaaat cctcctggtg gccaaaaacc tgagccatga attgacagcc
     1621 tgtaacactg ccgtatactg gaagaaagag acgaggaggg cgctatgaag gcattattta
     1681 ttggtggaac gggaacaatt agtcaaggtg ttgctgtacg cgctcgggag gctggctggg
     1741 atgtgtggtt gttaaaccgc ggaaaccggc aagaccgtgt tgttagcgga gtaacctctc
     1801 ttaagggaga tatcaacgcc gatgaagcag gcgtcgctgc cctcattaag gaaatgcact
     1861 ttgatgtagt agtgaatttt atagcctttg tgcctgaaca acttgaaagg gacgttcggc
     1921 tttttaccgg aaaaaccgga cagtttatct ttatcagctc agcctccgca taccaaaagc
     1981 cgcttaatca ttaccgaata accgaatcga ccccattggt aaaccctgta tgggaatatt
     2041 cccgtaataa aattgcctgc gaagaatttt taatggatca gtataggaca aaggggttcc
     2101 ccgtaacgat tgtacgacca agccatacct atgatgacag ataccttccg gtggcaatac
     2161 atggcaaaaa aggcagctgg caagtagtgg atcggatact caaggacaaa ccggttcttg
     2221 ttcatggaga cggaacttcg ttgtggacct tgacccatag ttctgatttt gcaaaagcgt
     2281 ttgtcgcttt gttaggaaac ccactggctc ttggagagac ctttcatatc accagtgacg
     2341 aatcgctttc ctggaatcag atatatgata gtttgggttc tgcccttggg aaaacagttc
     2401 acaagtatca cgttgccact gatttccttg cagcctgcga tcctgaatat agcgggacgc
     2461 tcctgggaga caaggcacac accgtagtct ttgataatac aaaaataaaa aggatacttc
     2521 cggattttac cacttcagta tcctttgccg aaggagcccg ccgctgtgtt cgctatttat
     2581 tggataacga acagtcccag ataccagacc ctgattttga cgctttttgt gagcgaataa
     2641 tccaggtaca agaagcggcc tgttctgcgt attttgcgag cataacccga taagaccccc
     2701 agcatctatt tctttttcaa aggaggggca atgaagcctc ttgatcctcg cttggctaat
     2761 ttttcgacaa aggatatcgc gtcagagtca gtcccttccg cgaagccata atgagtcgga
     2821 atgccgatag aagcctttgt atcaaggaca gactctaccg cgtcatcgat tgaacgcatc
     2881 gtgtgttttt gcccaagagg cacgatagca atatcaca
//
LOCUS       DAOQXQ010000151         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36738_length_2906_cov_3.088039, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000151 DAOQXQ010000000
VERSION     DAOQXQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36738_length_2906_cov_3.088039"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH81_03335"
     CDS             <1..181
                     /locus_tag="PKH81_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97099.1"
                     /translation="SLDRGIQSMERTLGEYERKVAHYQELMETQRRNIQAAQEERAAL
                     EESRQIAEKGLSDIR"
     gene            235..2052
                     /locus_tag="PKH81_03340"
     CDS             235..2052
                     /locus_tag="PKH81_03340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNQ97100.1"
                     /translation="MAKKTADYDESKIKTLSSLEHIRLRTGMYIGRLGDGTNPDDGIY
                     ILVKEVIDNSIDEFIMGNGSRIDIGLSEQKVSVRDFGRGIPLGKLVECVSVINTGAKY
                     NDEVFQFSVGLNGVGTKAVNALSSWFRVLSVRDGKCAEAIFERGVLKTNRTGSVKSDV
                     KNGTLVEFIPDEKLFGDYKFNLEYLERRIWNYAYLNAGLTLNFNGEKYISKNGLLDLL
                     ESELAEDHIYQIGYYKGKQLEFGFSHSNNYGENYFSFVNGQYTSDGGTHLSAFKEGFL
                     KGINEYYRKNYKSEDVREGTAAAVAIKVQSPIFESQTKNKLGNTEIRAWIVNETKAAV
                     DDWLHKNAEAARFLEQKILANEKLRTELNSVKKEAKEAAKKIALKIPKLKDCKFHLQD
                     GKKGDTSMIFITEGDSASGSMVSSRDVYTQAIFAMRGKCENMYGKKRADIYKNAEMYN
                     MMMALGIENDIDGLRYSKIVIATDADNDGFHIRNLLLTFFLSFFEELVTSGRVYILET
                     PLFRVRTKKETIYCYSEKERDEAVKKLGSASEVTRFKGLGEISPGEFGQFIGDDIRLL
                     PVSVQSLKNVPGILEFYMGKNTPERREFIMKNLLAEVDA"
     gene            2052..>2906
                     /locus_tag="PKH81_03345"
     CDS             2052..>2906
                     /locus_tag="PKH81_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNQ97101.1"
                     /translation="MDYIKTLFNRNFLEYASYVIRDRAIPDLEDGLKPVQRRILHCLF
                     EMDDGKFHKVANVVGSCMKYHPHGDASIGSALVVMANKSLFIDKQGNFGNILTGDGPS
                     AARYIECRITSLAKDLMYNPAITPMVPSYDGRNQEPVAFRAKLPLVLLTGAEGIAVGM
                     STYILPHNIAEVIEAEKQCLSGKSFKLYPDFLCGGLVDVSQYADGQGKVLARARLDTS
                     DDKRIVIRELPFGSTTESMIASIENAAKSGKIKISEINDFTTDKVEIEIKLQRGVYSP
                     DILDALYAF"
BASE COUNT          855 a          569 c          710 g          772 t
ORIGIN      
        1 ctctctcgac aggggtatac agtccatgga gcgcacgctt ggagaatatg agcgtaaggt
       61 agctcactat caggaactga tggaaacgca acggagaaac attcaagccg cacaagagga
      121 aagagccgct ttggaagaat cccgtcaaat cgcggaaaag ggattatccg atattcgcta
      181 gcttccttta ttagagatga ctcatttgat cttttctggt atactttttc cgttatggcc
      241 aaaaaaacag cagactacga tgaatcaaag ataaaaaccc tgagttctct tgaacatatc
      301 cgtttgcgaa cgggtatgta cattggacgc cttggtgatg gtactaatcc tgacgatggt
      361 atctatattc ttgttaaaga agtaattgat aactccattg atgagtttat tatgggcaac
      421 ggaagtcgaa tcgatatcgg cttaagtgag caaaaggtga gtgtccgcga ctttggacgc
      481 ggtattcccc tgggaaagct tgtcgagtgt gtttcggtaa taaatactgg agctaaatat
      541 aacgacgagg tctttcagtt ctctgttggt ctaaacggtg tgggtaccaa ggcggtaaac
      601 gcactgtcca gctggtttcg tgttctatct gttcgcgatg gaaaatgtgc agaggcgatt
      661 tttgagcgcg gtgtattaaa aaccaatcga accggttcgg ttaaaagcga tgttaaaaat
      721 ggaacccttg tagaatttat tcctgatgaa aaactatttg gggattataa gtttaatctt
      781 gaataccttg aaaggcgaat atggaattac gcgtatttga atgcgggcct tacgcttaac
      841 tttaacggtg aaaagtatat atccaaaaac ggcttgcttg acttgcttga atcagaactc
      901 gcagaagatc acatctatca gattgggtat tacaagggca agcagcttga gtttggtttt
      961 tctcattcaa ataattatgg cgaaaattat ttttcgtttg taaatggtca atatacatcc
     1021 gatggtggca cgcatctttc cgcttttaag gaaggcttcc tgaaaggaat taacgaatat
     1081 taccgaaaga attataagag tgaggacgta cgtgagggaa ctgctgccgc tgttgcgatt
     1141 aaggttcagt cgcccatctt tgaaagtcaa accaaaaata aactgggtaa taccgaaatc
     1201 cgcgcctgga ttgttaatga aaccaaggcg gcagtggacg actggcttca taagaacgcc
     1261 gaagctgcac ggtttttgga acagaagata ttagcaaacg agaagctccg caccgaatta
     1321 aactcggtaa aaaaagaagc caaggaagcg gcaaaaaaaa tagccctcaa gattccaaag
     1381 ctcaaggatt gcaagtttca tttacaggat ggcaagaagg gcgacacttc catgattttt
     1441 attacggaag gagattcagc ctcaggctcc atggtttcca gtcgcgacgt atatacgcag
     1501 gcaatatttg ctatgcgtgg taagtgcgaa aatatgtacg gtaaaaaaag agccgacatt
     1561 tacaagaacg cagaaatgta caacatgatg atggctcttg gcattgaaaa cgatatcgat
     1621 gggctcagat attcaaaaat tgtcatcgct accgatgccg ataatgatgg atttcatatc
     1681 cgaaatctgt tgttaacctt cttcctcagt ttctttgagg aactggttac ctcgggacga
     1741 gtatatatcc tggaaacgcc cttgttccgt gttcgcacaa aaaaggaaac gatttattgc
     1801 tatagcgaaa aggaacgtga cgaggcagtc aagaaacttg gctcggcaag cgaggttacg
     1861 cgctttaagg gtcttggaga gattagtcca ggagagtttg gccaatttat tggcgatgat
     1921 atacggctgc ttccggtgtc tgttcaaagt ttaaaaaacg taccgggaat tcttgagttt
     1981 tatatgggca agaatacgcc ggaaagacga gagttcatca tgaagaatct gcttgcggag
     2041 gttgatgcgt aatggattat ataaaaaccc tttttaaccg caacttcctt gagtatgcca
     2101 gctatgttat acgcgatcgg gcaatacccg atcttgaaga tggactaaag ccggtacagc
     2161 ggagaatact gcactgcctg ttcgagatgg acgatggaaa atttcacaag gtagcaaacg
     2221 tagttggctc gtgcatgaag taccatcctc atggagatgc atccattggt agcgcccttg
     2281 tggttatggc caacaagagt ttgtttatag acaagcaggg taattttgga aacattctta
     2341 ccggcgacgg tccttcggcg gctcgatata tcgaatgtcg tataacatcg cttgccaaag
     2401 acttgatgta taatcctgcc ataacaccaa tggttccctc ctatgacgga agaaaccagg
     2461 aacctgtcgc cttccgtgca aagcttccat tggtcttatt aaccggagca gagggtattg
     2521 ccgtcggaat gtctacgtat attcttccgc acaatattgc cgaggtaatt gaggcggaga
     2581 aacaatgtct gtccggtaag tcctttaagt tgtatcccga ctttttatgc ggcggactgg
     2641 tggatgtttc gcaatacgcg gacggacagg gaaaggttct cgctcgggcg cgccttgata
     2701 cctccgatga caagcgaatt gttatccgtg aattgccctt tggcagtaca acggagagta
     2761 tgatcgcatc gatagaaaat gcggcaaaat cgggaaagat aaaaatttcc gaaatcaatg
     2821 attttaccac cgacaaggta gagatagaga taaagctcca gcgtggagta tactctcccg
     2881 atatactcga tgccctgtat gccttt
//
LOCUS       DAOQXQ010000152         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_36752_length_2905_cov_3.263860, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000152 DAOQXQ010000000
VERSION     DAOQXQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36752_length_2905_cov_3.263860"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKH81_03350"
     CDS             complement(<1..208)
                     /locus_tag="PKH81_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97102.1"
                     /translation="MPVIRTNTTPQISPSIPAGIRIRPEHQRGVLPGCAGERSARRED
                     NISRRFQRQRVIISQSIGSHGKVAR"
     gene            complement(251..2374)
                     /locus_tag="PKH81_03355"
     CDS             complement(251..2374)
                     /locus_tag="PKH81_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ97103.1"
                     /translation="MSISKSAVSRPITVAILFCVLFLLGLYSAFDLPLDLLPDIELPY
                     VAISTRYDGAGPEEVEKRVTRVIESTISGVSGIEYLGSQSSSGNSLVYLQLTFGMNLD
                     EAMNDIRAKLDYVKEFLPADAKTPMIYKMDPNMFPIMFYAISGNRTPEELRDFADDLT
                     PKLEQVDGVASCYVQGGRERAIVVEIPRDRLEAYNLTITQIAQIIGAQNIQIAGGTIT
                     ESDLNYTINTVGEYTNVEDIKNTVISYKQAGSAQNAMMGGPMPTMTVVRLRDIADVYD
                     GFKDQSSLYYFNGVPCVQLAIQKQSGTNSVQSAKAVQARMEKIIAESPSDITITETRN
                     TTDIIQKSVDNVKSSAVQGALFAIIVLFIFLRSLRSTTIIGITIPLSIVITLGIMFFT
                     GMTLNLMTLAGLALGVGMLVDNSIVILENIYTYSEKGAKPKPAAILGSQEMIMAISAS
                     TLTTICVFLPLIMYKKELEIIGEILQGLAFTVVISLLCSLVVAVILVPVLSSHFFKIG
                     RKKDQVKTGLLGRWDTKLGVLFEKLDTKYFNSLKWVLKHKTQTILVIVGLFVLSLALL
                     PVLGFEYMPKQAQDAIQVDLTLPVGTKLETTEAVLRQLEEFVKQDIKGYKNISLLAGE
                     SEAWGMGGTSSYKGNLRINLPDLKDRIESEDEIKAKLRARFGAFPGASFTLSTGRQGG
                     AIQLNTGVVISTSSLITGSITISDR"
     gene            complement(2371..>2905)
                     /locus_tag="PKH81_03360"
     CDS             complement(2371..>2905)
                     /locus_tag="PKH81_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ97104.1"
                     /translation="SVGKVSKMESLEIGMSVPERFVSKIKEGQKAYLRFDAYPSQVFI
                     AKIIEVSPVLDQSSRTMGAKLALIERDDRIKAGMFARIKLITDTKTSTVKIPASAIVS
                     RFGDNFVFVVDGEEKNSVRKQPVTVGIRVDDKAEILSGVKANEEVIVRGQTLLEDGSR
                     INIVSRMESLPEMESNK"
BASE COUNT          685 a          686 c          755 g          779 t
ORIGIN      
        1 ggcgggcaac ctttccgtgg gaaccaatgc tctgacttat aatgacccgc tgacgctgaa
       61 aacggcggga aatattgtct tctcgtcggg cgcttcgctc accggcacag ccgggcagaa
      121 cgcctcgctg gtgctctggt cggatgcgga tgccagcggg gatggaagga cttatctgag
      181 gggtagtgtt agtacgaatg acgggcatat ctgggcaggt ggcggcagtg gttcagcaac
      241 ctggaacggg ctaacggtcg gagatggtta tgctgccagt aatcagggag gaggtactga
      301 ttactacgcc ggtgttcagc tgaatggcgc caccctgtct tccggtgctc agcgtaaagc
      361 tcgcgccggg gaaggcccca aaacgggcgc ggagttttgc ttttatttcg tcttcgcttt
      421 cgatgcggtc ttttagatcg ggaaggttaa ttcgcaagtt acccttgtaa gagcttgttc
      481 ctcccattcc ccatgcctcg gactctccgg caagaaggga tatgttttta tatcctttta
      541 tgtcctgctt tacaaattcc tctaattgac gaagtaccgc ctctgtcgtt tccagcttgg
      601 ttcctactgg cagggtaagg tctacctgga tggcatcctg cgcctgtttg ggcatgtatt
      661 caaaaccaag aacgggtagc aaggcgaggc tcagcacaaa caagccaaca ataaccagaa
      721 tggtttgggt cttgtgctta agaacccact tgagggagtt aaagtatttt gtgtcgagct
      781 tctcaaaaag aactccgagc tttgtatccc agcgtcctag taatccggtt tttacctgat
      841 ctttttttcg gccaatttta aagaagtggc tcgaaagaac aggtactaaa ataacggcta
      901 ctaccaaaga acagaggagt gaaataacaa cagtaaaggc aagaccctgg agtatttctc
      961 caataatctc gagttccttt ttgtacatga taagtggaag gaatacgcag attgtggtaa
     1021 gggtggacgc ggagatggcc attatcattt cctgcgatcc aagaatggcc gcgggctttg
     1081 gcttggcgcc cttttcgctg taggtataga tgttttccag gataacaatg gagttgtcca
     1141 caagcatacc aacaccaagg gcaagtcctg caagggtcat caggttaagc gtcatgccgg
     1201 taaagaacat aatgcccagg gtgataacga tggaaagggg aatggttata ccgatgatgg
     1261 ttgtgctgcg aaggctccgc aggaagataa acaaaacgat aatggcaaag agggctcctt
     1321 gaaccgcgct tgactttacg ttgtctaccg acttttgaat aatgtctgtg gtgttcctcg
     1381 tttcggtaat cgtgatgtcg gacgggcttt ctgcgattat tttttccatt cgcgcctgta
     1441 cagccttggc ggactgaacc gagttggttc cgctttgttt ttgaatggca agctgcacgc
     1501 agggaacgcc attaaaataa tacagcgaag actggtcctt aaacccgtcg tatacatccg
     1561 cgatgtctct aagcctgaca accgtcatgg tgggcattgg gccacccatc atggcgtttt
     1621 gcgcagagcc tgcctgtttg taggaaatta ccgtattctt gatatcctct acattcgtgt
     1681 attcgcctac agtgttgatg gtataattaa ggtcggactc ggtaatggtt ccgccggcta
     1741 tctggatatt ctgcgcaccg ataatctgcg caatttgggt aatggtcagg ttatatgctt
     1801 caagtcgatc ccggggaatt tcaaccacga tagcccgctc acggccaccc tgtacgtaac
     1861 aggaagcgac gccgtcaact tgctccaatt tgggtgttaa gtcgtcggca aaatcgcgta
     1921 gttcctcggg ggttctgttt ccgcttatag cgtaaaacat aatcgggaac atgttcggat
     1981 ccatcttgta gatcatcggt gttttagcgt ccgcaggaag gaattccttg acgtaatcga
     2041 gcttcgcccg aatgtcattc atcgcctcgt ccagattcat tccaaaggta agctggaggt
     2101 ataccaggct gtttccgctg gaggactgcg agcccaggta ttcaataccc gagactcctg
     2161 agatggtgct ttctataacg cgggttacgc gcttttctac ctcctcgggg ccggctccat
     2221 catagcgggt acttattgct acatacggaa gttcaatatc cggtaaaaga tcaagcggaa
     2281 ggtcaaaggc cgagtacagg cctaggagaa acaaaacgca aaagagaata gcgaccgtta
     2341 taggccgcga aaccgcagac tttgatatac tcatttattg ctctccatct cgggaagact
     2401 ctccattctg gatacgatgt tgatgcgcga accgtcttcc aaaagggttt gtccgcgaac
     2461 aatcacttct tcgttggctt ttacgccgct caggatttcc gccttgtcgt caacgcggat
     2521 gccaacggtt actggttgtt tgcgtaccga atttttttct tccccatcca ccacgaacac
     2581 aaagttgtcg ccaaagcgcg acacgatggc cgaggcggga attttaaccg tggaagtttt
     2641 agtatcggta atcaatttga ttcgggcgaa cattccggcc ttgattcggt cgtcgcgttc
     2701 gataagtgca agcttggcgc ccatggttct ggagctttgg tccaggacag ggcttacctc
     2761 aataattttt gcgatgaata cctgggatgg gtatgcgtca aaacggagat aggctttttg
     2821 tccttccttg atttttgata caaagcgttc gggtacgctc ataccaattt ccaggctttc
     2881 catcttgctt acctttccta ccgaa
//
LOCUS       DAOQXQ010000153         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37133_length_2885_cov_3.144170, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000153 DAOQXQ010000000
VERSION     DAOQXQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37133_length_2885_cov_3.144170"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /gene="yajC"
                     /locus_tag="PKH81_03365"
     CDS             <1..287
                     /gene="yajC"
                     /locus_tag="PKH81_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNQ97105.1"
                     /translation="VFVIFYLFIIRPQNKKQKETERMISAIKKGDKIITIGGIHGEVT
                     STKEQTVIVKVDEACKLEFSRSAIASVVTDEKPGKKDKKSVPDAASGNAN"
     gene            353..2086
                     /gene="secD"
                     /locus_tag="PKH81_03370"
     CDS             353..2086
                     /gene="secD"
                     /locus_tag="PKH81_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ97106.1"
                     /translation="MSKRSRLVLILVVIAVCFAFLWPSIKWYYVTPKEDQALALGSRE
                     KIKDYARNMAVADLKQLISDARADSQDPLNAKFVPLLKEAEKNYKAMKKTVPSVWTPR
                     VLLAAFPSESDVMSILENQYRDTILSLKKTQSSAIKLGLDLSGGMSIIIKADLDAAVA
                     TQKDSSVDSGVFRQEAMMQALDTLNSRIDKFGLSEPVIRRQGEDRIYIEIPGTSDSDR
                     INSIIMGKGMLAFHLVDEESTASFRAYLEQNPTSTFDAEYNLVNPGIIPANRRVLGLY
                     NKDEYGLDERIGFLVVHSDMALEGKHIQSAETRRDEMGRPVVNFILDSEGSKIFAEVT
                     ANNVNKGLAIVSDDKIKSYATINEAIPTGSVQISGFSAEEAENLKAVLRTAWLNVPLE
                     LENQQVIGASMGEESIRQGVWAICGGLAAVFVFMLLYYRGAGINAVVAQILNFYIIFS
                     VLSALNLTLSLSSIAGMILTIGMAVDANVIIFERIKDELRLGKGRVASISSGLDHAFW
                     AIMDSNITTFIAAIFLSQLGTGAIKGFAYSLAIGVVSSVFTALVVSRLIFDFNTDVLK
                     KKTVSISWRIK"
     gene            2086..>2885
                     /locus_tag="PKH81_03375"
     CDS             2086..>2885
                     /locus_tag="PKH81_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNQ97107.1"
                     /translation="MKRVVTFSKAFIPSVIISAILIGSGLFGYVTKGINYGLDFQGGL
                     IQQVRIAPTALSLSYVGSQSVTVQQSSQSIDLVVTGVGSDNQTYRFPYSRFEIVGDFV
                     REVQTVPGVSVSLESPMLVPLKSLFVDSEASARLSSVPYRFHYIPEGAPLISADEVRQ
                     AISAIPSASVQLVGNPADRAFQIRLKDDASDPQASQNLKQTLTSLLVSRWGEPSIAII
                     STDFVGSRFSASLASQAFWLVGATLLLIWVYCAIRFRWDFAVGGVLAI"
BASE COUNT          679 a          673 c          709 g          824 t
ORIGIN      
        1 ttgtgtttgt cattttttac cttttcatca tcaggccgca aaataaaaag caaaaagaaa
       61 ccgaacggat gatctctgcc ataaaaaaag gtgacaagat tatcaccatc ggtggtattc
      121 acggcgaggt tacctctact aaagaacaaa ccgtcatagt caaggttgac gaggcatgta
      181 agctcgagtt cagccgttct gcgatcgctt cggttgttac cgacgagaag ccgggaaaaa
      241 aagataaaaa atcagttccg gacgctgctt ccggtaacgc taactgacac atcaatacat
      301 tggggaggcc tcttctcggt aagaggtctt ctgtcatatc tggagtttta ctatgagcaa
      361 acgaagcagg ttagtactta tcctggtggt tatcgcggta tgtttcgcgt ttctgtggcc
      421 gtccatcaag tggtattatg ttacaccgaa agaagaccag gcccttgccc tgggttcccg
      481 cgaaaaaatc aaggactatg cacggaatat ggcagtagcc gatcttaagc aattgatcag
      541 cgatgccagg gccgacagtc aggatccgct taatgccaag tttgttccgc ttcttaaaga
      601 agctgagaaa aactacaagg cgatgaaaaa aacggttcct tccgtatgga cgccaagagt
      661 gcttcttgcg gcttttccgt ctgaatcaga tgttatgtcg attttggaaa accagtatcg
      721 agacaccatt ttgtcgctta agaaaaccca atcaagcgcg attaagctcg gtctggacct
      781 ctctggtggt atgagtatca tcatcaaggc ggatttggac gctgccgtgg caacccaaaa
      841 ggattcttcg gtagatagcg gtgtattccg tcaggaagcg atgatgcagg ccctcgatac
      901 ccttaatagc cgcattgaca agttcggtct ttctgaaccg gttattcgtc gtcagggcga
      961 agatcgcatc tatatcgaaa ttccggggac atccgattcg gaccgaatca attcaatcat
     1021 tatgggtaag ggcatgcttg cctttcattt ggttgatgaa gagtccaccg cttcgttccg
     1081 ggcgtacctt gagcaaaatc caacctctac ctttgatgca gaatataatc tggttaatcc
     1141 ggggataatc cctgcgaaca gaagggttct tggcttgtat aacaaggatg aatatggact
     1201 tgatgagcgc atcggcttcc tggttgtcca tagcgatatg gcccttgaag ggaaacacat
     1261 tcaaagcgca gaaacccgtc gcgacgagat gggccgtcct gtcgttaact ttatccttga
     1321 ctccgaagga tcaaagatat ttgccgaggt aacggctaac aatgttaata agggtcttgc
     1381 cattgtatct gatgacaaga tcaagtcata cgccacgatt aatgaagcta tccccaccgg
     1441 ttctgtccag atttccgggt tctccgcaga agaagcggaa aaccttaagg cagtactcag
     1501 gacagcctgg ctcaatgttc cccttgagct cgagaatcaa caagttatcg gcgcaagtat
     1561 gggtgaagaa tccattcgcc agggtgtatg ggctatctgt ggcggtcttg ctgcggtctt
     1621 tgtctttatg cttttgtact atcgcggcgc tggtattaac gcggttgttg cccagattct
     1681 taacttctat atcatcttca gcgttttgtc ggcgctgaat cttacccttt ccctttccag
     1741 tatcgccggt atgatcctta ccattggtat ggctgtcgac gcgaacgtta tcatctttga
     1801 acgaatcaag gatgagttac gccttggtaa gggcagggtt gcatcaattt cttccgggct
     1861 tgatcatgcc ttttgggcga ttatggactc gaacattacc acctttatag cggcaatctt
     1921 cctttcccag cttggaaccg gtgcaatcaa gggttttgcc tacagtcttg caatcggtgt
     1981 tgtatcatcc gtgtttaccg ccctggttgt atcccgtctt atcttcgatt ttaataccga
     2041 tgtgctgaag aaaaaaaccg tcagcatcag ctggaggatt aagtaatgaa aagggttgtt
     2101 acattcagca aggcttttat tccatccgtt atcatcagtg cgattcttat tggtagtggc
     2161 ctgttcggtt atgttaccaa gggtatcaat tatggtcttg atttccaggg tggtcttatt
     2221 caacaagttc gtatcgcacc tactgccctg tcgcttagct atgttggcag tcagtcggta
     2281 acggttcagc aatcttccca atcaattgat ttggttgtaa ctggcgttgg ttcggacaat
     2341 caaacctatc gcttcccgta ttctcgtttt gaaatcgttg gtgattttgt tcgagaggtc
     2401 cagacggttc caggagtgag cgtttctctt gaatcgccga tgctggtccc gctcaagtcc
     2461 ctgtttgtgg actccgaggc atctgcccgg ctttcctctg taccgtatcg attccattat
     2521 attccggaag gggccccgct tatcagcgcg gatgaggttc gtcaggcaat tagcgcgatc
     2581 ccctctgcct ctgtgcagct ggtgggaaat cctgccgacc gggcatttca gattcgactt
     2641 aaagatgatg cctcagatcc ccaggccagc cagaacctta agcaaactct tacatcgctg
     2701 cttgtttcaa gatgggggga gccttccatt gcgattatca gtaccgattt tgtaggttca
     2761 cgcttttcgg cctcgcttgc aagccaggca ttttggcttg tcggtgcgac gcttttgctg
     2821 atttgggtat actgcgcgat tcggttccga tgggacttcg ctgttggcgg tgttttggcc
     2881 atcat
//
LOCUS       DAOQXQ010000154         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37234_length_2881_cov_2.882166, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000154 DAOQXQ010000000
VERSION     DAOQXQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37234_length_2881_cov_2.882166"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PKH81_03380"
     CDS             complement(<1..1008)
                     /locus_tag="PKH81_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97108.1"
                     /translation="MIRIVNRAEINRTREHLWRTLSVALALCASAVIMALIGYNPLEV
                     YAKILQGSLKSAYRFKETIHKAIPLVTLSLGIAVAFRMKFWNIGAEGQFYLGAFGASL
                     VAFYAPPLSPWILLPLMLLSGFFLGGLWALVPALLKARLGASETLVTLMLNYVALKWI
                     SYLQYGPWKDPAASGFPKIAPFPDAAILPQVFGIHAGWLIALALCLVVHVMLSSSKLG
                     YEIDVLGESVATARYAGISVTRVTLIAIMISGGLCGIAGMMQASAIEHSLSEQLSGGL
                     GFTAVITTWLARLSPPVILVVSFLFSILIQGGSFLQSSLKIPASVSAILQGIILFFVL
                     GG"
     gene            complement(1005..2516)
                     /locus_tag="PKH81_03385"
     CDS             complement(1005..2516)
                     /locus_tag="PKH81_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97109.1"
                     /translation="MPYIELRNICKSFGAVIANRDVSLTVNKGEILALLGENGSGKST
                     LVNMLSGIYAPDSGTIIVDNKVVSFTSPRDAIRCGIGMVHQHFKLASVMTAAENISLG
                     EKGPLWIRRDRVAERIYQIEKRFGLVTNPDKKIYTMSVGEKQTVEILKVLYRGANVLI
                     LDEPTAVLTPQESNRLFDILRKMRDEGCSIIIITHKLSEVLAISDRVTVLRRGMSVGT
                     VETAATSARELTEMMVGGAVSLEIRRPRLELPAEPLLSISNLTVPGEEGRKALDRLSM
                     DVYPGEILGVAGIAGSGQKELCEAIAGLCKAAEGSIRFDGDEILSLSPRAIIRKGISM
                     SFIPEDRLGMGLVAGMNITDNVLLKSYTRSRGLFMDRGDGVRRAEHIVRHYDISTPSV
                     SHTVRKLSGGNIQKVLLGREIEMNPRLLITAYPVRGLDIGASMTIYETLNLQKERGVA
                     ILYIGEDLDVLLELCDRILVLHDGKVMDVVDGETATKEDIGLLMMGQKPEESV"
     gene            complement(2577..>2881)
                     /locus_tag="PKH81_03390"
     CDS             complement(2577..>2881)
                     /locus_tag="PKH81_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97110.1"
                     /translation="FYVDDVQRILNGTWTSRAYWEGYSKGMVTLDALSANAAAGTAEA
                     IEAAKAKIIDGSFEPFTGPIADQKGTVRVADGVKITDDEIWNMGWFVKGVIGNVPN"
BASE COUNT          784 a          768 c          684 g          645 t
ORIGIN      
        1 accgccaaga acaaagaaaa ggatgatgcc ctgcaggata gccgaaacgg aggcgggtat
       61 tttgagcgag ctttgcaaaa aagatccgcc ttgaataaga atggaaaaca ggaaggatac
      121 caccaaaata accggtggac ttaagcgcgc aagccaggtc gtaataacgg cggtaaagcc
      181 aaggccaccg gaaagttgtt cggaaaggga atgttcaatg gcagaggcct gcatcatccc
      241 ggctattccg cacaaaccac cggatatcat aatcgcgata agggtaaccc gggttacgct
      301 aatgcccgca tatcgggcgg tagcaacgct ttcgcccaac acatcaattt cgtatccaag
      361 tttactgctc gaaagcatca catgcactac caggcacagc gcaagggcaa taagccagcc
      421 ggcatgaatg ccaaaaacct gcggcaagat agccgcgtcc ggaaacggcg caatcttggg
      481 aaagcccgac gcagcgggat ccttccatgg accgtattga agatacgaga tccatttaag
      541 ggcaacgtag ttaagcatta aggttaccag ggtttcgctt gcacccaaac gagccttgag
      601 caaggcaggg accaaggccc acagccctcc taaaaaaaac ccggaaagaa gcatcagggg
      661 caacaatatc cagggcgaaa gaggcggggc ataaaaggca acaagagaag cgccaaaggc
      721 tcccaggtaa aactgccctt ccgcaccgat attccaaaat ttcatcctga aggcaaccgc
      781 gattcccaac gaaagggtta ccagcgggat tgccttatgt attgtttcct taaagcggta
      841 cgcgcttttt agtgaaccct gcagaatctt ggcgtatacc tcaagcgggt tatatccgat
      901 tagagccata ataaccgcgg aggcgcacag ggcaagagcg accgaaagcg ttcgccacag
      961 atgttcccgc gttcggttaa tctcggcgcg atttacaatt ctgatcatac actctcctcc
     1021 ggtttttgcc ccatcatcaa aaggccaata tcctcttttg ttgccgtctc tccatcaact
     1081 acatccatta ccttcccgtc atgaagcaca aggattcgat cgcataactc aagaagtaca
     1141 tcaaggtctt cgccaatata cagaatcgca accccgcgct ctttttgaag attcagggtt
     1201 tcatagatag tcatggaagc gccaatatca aggccgcgaa ctggataggc agttattaaa
     1261 agccggggat tcatctctat ctcgcggcca agcaatactt tttgaatgtt tccgccagag
     1321 agctttctaa ccgtatgaga gaccgacggg gtagagatat cataatggcg tacaatgtgc
     1381 tccgctcgtc gcacgccatc tcccctgtcc ataaaaagac cgcgggagcg ggtgtagctt
     1441 ttaagaagta cattgtctgt aatattcatg ccggcaacca aacccatacc aaggcggtct
     1501 tcggggataa aactcatgct gattcccttg cgaataatag cgcggggcga aagagataaa
     1561 atttcatccc catcaaagcg gatagaacct tctgcagcct tacatagtcc ggcaattgcc
     1621 tcgcatagtt ccttttgacc agaacctgca ataccggcaa ccccaagtat ttctcccgga
     1681 tacacatcca tagaaagtct atcaagggcc tttcgtccct cctctccagg aacagtcaag
     1741 ttgctgatcg aaaggagcgg ttcagcgggg agttccaacc gcggtcggcg aatctccaaa
     1801 gacactgcac cgccaaccat catctcggtt aattcgcggg cgcttgtagc agccgtttcc
     1861 acagtaccaa cgctcattcc gcggcgcaaa acggttactc gatcgcttat ggccaatact
     1921 tccgaaagct tatgggtaat gatgataatg gaacagcctt cgtcccgcat cttgcgcaaa
     1981 atgtcaaaca gccgattgct ttcttgcgga gtgagcaccg cggtaggctc atccaaaata
     2041 agaacattgg cgccgcggta gagtacctta agaatctcta ccgtctgctt ttcgccaacg
     2101 ctcatggtat atatcttttt gtccggatta gttaccagac caaatcgttt ttcaatttga
     2161 taaatgcgtt cggctacgcg gtcgcgtcgt atccatagag ggcctttttc gccaaggctg
     2221 atattttccg cggcggtcat aaccgaagcg agcttaaaat gctgatggac catcccgata
     2281 ccgcagcgaa tagcgtcgcg gggggatgta aaagaaacaa ccttgttatc tacaattatt
     2341 gtgccgctgt ccggagcata tatcccggag agcatattaa ccagggtgct tttccccgat
     2401 ccgttttctc ccaaaagagc tagaatctcg cccttgttaa ccgtaaggct cacatcccga
     2461 ttggcaatta ccgccccaaa acttttacag atatttctaa gttctatgta tggcatcagg
     2521 cggcagcctc ctcttataga aaaaaggcag ggaccaaagg cccctgccca tgaatatcag
     2581 ttagggacgt ttccgataac gccctttaca aaccaaccca tgttccagat ttcgtcgtcg
     2641 gttatcttga cgccatcagc aacgcggacg gttccctttt gatcggcgat ggggccggta
     2701 aagggttcaa aacttccgtc aataatcttg gccttggcgg cctcgatagc ctcggcagta
     2761 ccggcagcgg cattggcgga aagggcgtca agggtaacca tacccttgct atagccttcc
     2821 cagtaagcgc ggcttgtcca ggttccatta aggatgcgct gtacatcgtc cacgtagaag
     2881 g
//
LOCUS       DAOQXQ010000155         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37257_length_2880_cov_3.128850, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000155 DAOQXQ010000000
VERSION     DAOQXQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37257_length_2880_cov_3.128850"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PKH81_03395"
     CDS             <1..698
                     /locus_tag="PKH81_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ97111.1"
                     /translation="IAFGDSTNLKVGQKVLAIGNPFGLERTLTTGIVSALGRPIKTSS
                     NTVIKDMIQTDTAINPGNSGGPLLDTQGRMIGINTMIYSTSGSSAGIGFSVPVNTAKR
                     VVSELIQFGKVRRGSIDAELVQLSSPIATYARLSTDKGLLVSRVGPGSNAEKAGLKGG
                     SEAVRYGSGRSSSIIYLGGDVITSIGGQRVESLTDYYSALESKKPGDKVEITVMRGKQ
                     QISLSLLLAERTR"
     gene            720..2705
                     /gene="uvrB"
                     /locus_tag="PKH81_03400"
     CDS             720..2705
                     /gene="uvrB"
                     /locus_tag="PKH81_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNQ97112.1"
                     /translation="MRNFTVVSEYEPSGDQGEAIRALSEGLRSGDKFQTLRGVTGSGK
                     TFTMAKIIEQVQRPTLVISHNKTLAAQLYREFKTFFPNNAVEYFVSYYDYYQPEAYVP
                     SRDLYIEKDASINEEIDRLRLSATFSLMERRDVIVVSTVSCIYGLGLPESYRDLRIHI
                     DKGEVLDLDTFKKKLVSLQYERNDSVLERGRFRCRGDTIEVYPAYLEEAYRIELDWDT
                     VTRIRKFHPVSGEIILELDELSIYPAKHFVVNEEAIPNALERIREQLAEQLDRFKAQG
                     KILEAERLKTRTEYDLEMLAEMGYCPGIENYSAPIAQRPVGAPPATLFHYFPEDFLLF
                     MDESHVTWPQVGAMFEGDRSRKQNLVDFGFRLPCALDNRPLKIDEFEKMINQAIFVSA
                     TPRELEIQKSTRVVEQVIRPTGLLDPLIEVRPSEGQMEDIFREVQDRISKNERSLILT
                     LTKRMAEDLTDYLSSLSLKVKYIHSEVETFERVEILKGLRSGEFDILVGINLLREGID
                     LPEVTFIGILDADKIGFLRSTTSLIQIIGRAARNVNGQVVMYADRMSDAMKEAIGETS
                     RRRAIQEKWNTDHGITPTTIKKAVEDILVRENELKREAAQTESGVLVSSYNLLIPTER
                     KKLVRELEKQMAEYAELLQFEEAAVIRDKIVEIRAMD"
BASE COUNT          788 a          707 c          690 g          695 t
ORIGIN      
        1 cgattgcatt tggggattca accaatctta aagtaggaca aaaggtcctt gccatcggga
       61 acccctttgg tctggaacga actcttacca ccggtatcgt aagcgctctt ggacggccaa
      121 taaaaaccag ttcaaacacc gttatcaagg acatgattca aactgatacc gcaattaacc
      181 ccggaaactc aggcggaccc cttcttgata cccagggccg gatgattggc ataaacacca
      241 tgatctattc aacctccgga agttccgcag gcatcggttt ttccgttccg gttaatactg
      301 caaagcgcgt tgtttccgaa ctaatacagt ttggtaaggt acggcgcggc tcaatcgatg
      361 cagagctcgt ccaattaagc tcacccatag ctacctatgc ccgcctttcc accgataagg
      421 gacttcttgt atcccgtgtt ggccctggca gtaatgcaga aaaagcaggc cttaaaggag
      481 gtagcgaagc ggtacgctat ggctcgggtc gctcgagctc gattatttac ctgggtggag
      541 acgttataac ctccataggc ggtcaacggg tggaaagctt aaccgactat tactccgcac
      601 ttgaatcaaa aaaacccggg gataaggtag agattaccgt aatgagagga aagcaacaaa
      661 tttccctttc cctgttgctc gctgaacgca cacgctaaac gaagacgagg ggtacagcga
      721 tgagaaattt taccgttgtt tctgagtatg aaccctcggg ggaccaggga gaggccattc
      781 gggccctctc cgagggtctt cgaagcggtg ataagtttca aacgctcaga ggggttaccg
      841 gtagcggtaa aacctttacc atggcaaaga ttatcgagca agttcaacga ccgaccctgg
      901 taatcagcca taacaagacc cttgcggcgc agctctaccg tgagtttaaa accttttttc
      961 ccaacaatgc cgttgagtat tttgtctcgt actacgatta ctatcaaccg gaggcttatg
     1021 ttccctcgcg cgatctctat atcgaaaaag acgcttcaat aaatgaggag atagaccggt
     1081 tgcgactttc cgcaaccttt agccttatgg aacgccgcga tgttattgtg gtctccacgg
     1141 tctcttgtat ctatgggttg ggtttacccg aatcataccg tgatttacgt attcatattg
     1201 acaagggaga ggttctggat cttgatacct ttaaaaagaa actggtatct ctgcaatatg
     1261 agcgcaatga ttcagtgctt gagcgcggac gatttagatg ccggggcgat acaatcgagg
     1321 tataccctgc atatcttgaa gaagcatacc gcattgaact ggattgggac accgtaaccc
     1381 gcattcgtaa attccatcca gtgtccggag agattatcct tgaactggat gaactttcca
     1441 tctatccggc aaaacacttt gtagttaacg aagaagcaat accaaacgcc cttgagcgaa
     1501 ttcgcgaaca actggcggag caacttgatc gattcaaggc tcaggggaaa atactggaag
     1561 cagaacgatt aaaaacaaga actgagtatg accttgagat gcttgccgag atggggtatt
     1621 gccccggaat agaaaactat tcggcaccta tcgcacagcg tcctgtagga gctcctccgg
     1681 caacattgtt ccattacttc cccgaagact tccttctttt tatggacgaa agtcatgtca
     1741 cctggcctca ggttggcgcc atgtttgagg gagaccgctc aagaaagcaa aatctggtag
     1801 actttggatt tcgcctcccc tgcgctcttg ataaccgacc gctcaagatt gatgaatttg
     1861 aaaagatgat taatcaggcg atttttgtat ccgcaacccc gcgtgaactg gagattcaaa
     1921 aatccactcg cgttgttgag caagttatcc gtcccaccgg tcttcttgat ccccttatcg
     1981 aggttcgtcc cagcgaagga caaatggaag atatcttccg ggaagtgcaa gatcgtatca
     2041 gcaaaaatga gcggagcctc atccttaccc ttaccaagcg catggcagag gacttaacag
     2101 actatctttc ctccctttcg ctcaaggtaa aatacatcca ttcggaagtt gagacctttg
     2161 aacgcgttga aatacttaaa ggcctgcgct ccggagaatt tgatatcctg gtgggaatca
     2221 atcttcttag agagggcatc gacctccccg aggttacctt tatcgggata ctggatgcag
     2281 acaaaattgg ctttttgcga tccactacca gcttaattca gataatcggg cgagccgcac
     2341 gaaacgttaa cggacaggtt gttatgtacg cagaccgcat gagcgacgcg atgaaggaag
     2401 ccataggcga aacaagcagg cggcgcgcca ttcaggaaaa atggaatacc gatcacggca
     2461 ttacacccac caccatcaaa aaggctgtgg aagatatcct ggttagagaa aacgagctta
     2521 agcgggaagc ggcgcaaact gaatcggggg tactcgtatc aagttacaac ctgcttatac
     2581 cgacagaacg aaagaaactc gtccgggagc tggaaaaaca aatggccgaa tacgccgagc
     2641 tcttgcaatt cgaagaagcc gcggttatcc gcgacaagat tgtcgaaata cgggcaatgg
     2701 attgacggtg gagcttaatt actgctcgca ggattgttgg taatcttgcg tagcaatttc
     2761 cttgtttggc gtggtgatac accttaccat cagtcgctcc tattccacaa agcatagccg
     2821 atttctcccc tacaccagca aatgattcgc cgtccaccca ttccccggct ggtttttgat
//
LOCUS       DAOQXQ010000156         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37591_length_2863_cov_2.748575, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000156 DAOQXQ010000000
VERSION     DAOQXQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37591_length_2863_cov_2.748575"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKH81_03405"
     CDS             <1..251
                     /locus_tag="PKH81_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97113.1"
                     /translation="MVQIRGKKDSYPLVQIYEGLPESRILKIDASRETWEHALLAYQE
                     GDLPRARALFELVVEEDPEDLAANYLFIQVTNELYTSI"
     gene            complement(255..1061)
                     /locus_tag="PKH81_03410"
     CDS             complement(255..1061)
                     /locus_tag="PKH81_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNQ97114.1"
                     /translation="MKSPKELSIEKKILEQLTPGSPSWKLAESLVNDPEIQYMQEYAN
                     TVSILRLGYNDHGPVHMRTVTRNAVLMMRLLHEGGIQTSIEAEKTGNHEESLCAVILA
                     AFLHDLGMAMGRVDHELNSVSLAKPLIDRVLKEVFPENMEKRVVISSVALEGIFGHMA
                     THRINSVEAGVILIADGCDMEKGRARIPLVLNSEPRAGDIHKYSASSIEKVHIEKGES
                     NPIRISVEMSSEVGFFQIEEVLLPKVNMSPVKKHIELFAGLSGQEMKKYL"
     gene            1208..1582
                     /locus_tag="PKH81_03415"
     CDS             1208..1582
                     /locus_tag="PKH81_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97115.1"
                     /translation="MSERTVLVVDDSRIMRNIVKNTFATMGIPCQFLEAGNGREALEQ
                     LEKNKVNLVLLDWNMPELSGLDFLKKVRAMEGYKDLPIIMVTSEAARYNVIEALKSGA
                     TDYIIKPVNERVFKERLSKLEI"
     gene            1615..2091
                     /locus_tag="PKH81_03420"
     CDS             1615..2091
                     /locus_tag="PKH81_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNQ97116.1"
                     /translation="MEKYIQPFVDVCVNVFRDFVGAELTPERPHFSNKDDLNEWDISA
                     VIGLTGEARGAVVISMKKQLACHLTGTLTGATHSDLDEEVVDAIGEIINIIAGNAKRG
                     LEEAFRLVISLPTIVKGPGHSVQWPNGQARIICIPFHIFESETFCLSVAIEASNGA"
     gene            2093..>2863
                     /locus_tag="PKH81_03425"
     CDS             2093..>2863
                     /locus_tag="PKH81_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97117.1"
                     /translation="MQASEKKQKMNIFLSIMTSGKPFYQRDEATMDTMVRHILLNSMI
                     FLGGTLLVIFGLESIKSGAPIQGMFDLSMAGMTLLAFVILRTAAPFILSGFMTVVPYM
                     MLCAFLAVSGGVQGSGVLWIYSFPLLSIFLLGMKSGIALSTVLLFIVSAAVFIPGVSP
                     MKFETSFALRTVGVYILVLVCTIVYEQTKLVKDRWVARLTNALQVERDEITAMKDNLK
                     VGLFLINRDYIIQPQYSKALETVMAETDLNGKNFIDVLS"
BASE COUNT          702 a          629 c          700 g          832 t
ORIGIN      
        1 gtatggttca gatacgagga aaaaaggatt cttaccctct ggtacagatt tatgagggtt
       61 tacctgagtc gcgaattctc aagatagacg caagccgtga gacttgggaa catgcgcttt
      121 tggcgtatca ggaaggggat cttccacgcg ctcgcgcctt gtttgaactt gttgtggaag
      181 aagacccgga agatttggcc gccaattacc tgtttattca ggtaaccaac gagctgtata
      241 cctcaatcta gggcctacag gtattttttc atctcctgtc cgctgaggcc ggcaaatagc
      301 tctatgtgtt tttttaccgg actcatattc accttgggaa gaagcacttc ttctatctga
      361 aaaaatccaa cttcgctgga catctctacg cttatcctga tcggatttga ttcacccttt
      421 tctatgtgaa ctttttcgat ggaggaggcg gaatacttgt gaatatcacc ggcccttggt
      481 tcgctattca ataccagggg gattcgagcc cgtccctttt ccatgtcgca tccatctgca
      541 ataaggatta ctcccgcttc taccgagttt atccggtgcg tcgccatgtg gccaaagatt
      601 ccttccaggg caacagagct gataacgacg cgcttttcca tgttttccgg gaagacttcc
      661 ttgagaacac ggtctattag gggttttgcc aggcttacgc tattaagttc atgatctacc
      721 ctgcccatag ccatgcccaa gtcatgtaaa aaagctgcaa ggattaccgc gcataagctc
      781 tcttcgtggt ttcctgtttt ttctgcttcg atactggttt ggatgccccc ctcatgcaaa
      841 aggcgcatca taagaactgc gttgcgggtc acggttctca tgtgaacggg tccgtggtcg
      901 ttatatccca ggcgcagaat ggacacggta ttggcatatt cttgcatgta ctgaatttcc
      961 gggtcgttta caaggctttc ggccagtttc catgagggag accctggagt gagctgctcg
     1021 agtattttct tttctatgga aagttctttc ggcgatttca tatgatatga atatactccc
     1081 ccccagtcgc atccggcaag tggcggaaaa agtggaaata atggagaaaa cagcaaaggt
     1141 taacatggaa atttctgaat agagggtata tactgtgcgc atactaatat aaaagtttgg
     1201 gggacgaatg agcgagcgta ctgtacttgt tgttgacgat tccagaataa tgcggaatat
     1261 cgtaaaaaac acctttgcaa cgatggggat tccctgtcag tttcttgaag ccggaaatgg
     1321 acgcgaagcg cttgagcagc tcgaaaaaaa caaggttaat cttgttttac ttgactggaa
     1381 catgccagag ctatccggtt tggactttct aaaaaaggtt cgcgctatgg aagggtataa
     1441 ggatttaccg attattatgg tcaccagcga agctgcccgg tataacgtta tcgaggcgct
     1501 taagagcgga gcgacggatt acattattaa accggtaaac gagcgcgtat ttaaggaacg
     1561 tctttctaaa ctcgagatct aatcgggtgt ccaatcaaga ataaggagtc tgtagtggaa
     1621 aaatatattc agccttttgt agatgtgtgc gtcaatgtgt tcagggattt tgttggggcg
     1681 gagctaaccc ccgagcgccc acacttctca aataaagacg atttaaacga gtgggatatt
     1741 tctgcggtta ttggtttgac tggagaggcg cgcggtgccg ttgttatttc catgaaaaaa
     1801 cagcttgcct gccacctgac cggcacctta accggcgcga cacattccga tttggatgaa
     1861 gaagtcgtag acgctattgg cgagataatc aatattatcg ccggtaatgc aaagcgaggg
     1921 cttgaagaag ccttccgtct ggttatctct cttccgacta tcgttaaggg tccaggtcat
     1981 tcggttcaat ggcctaacgg tcaagcccga attatttgca ttccctttca tatctttgaa
     2041 tcggaaactt tttgtctgtc ggttgccata gaagcttcca atggagcgta gtatgcaggc
     2101 tagtgaaaaa aaacaaaaaa tgaatatctt cctctcgatt atgacgagcg gaaagccctt
     2161 ttaccaaagg gatgaagcga ccatggatac catggtccgc catattttgc ttaattccat
     2221 gatattcctg ggcggcacgc ttctggtaat atttggactg gaaagtatta aaagcggcgc
     2281 tcccattcag ggtatgtttg acctttcgat ggcgggaatg acgctgcttg cttttgttat
     2341 ccttagaacc gccgccccgt ttatcctctc ggggtttatg accgttgtcc cgtacatgat
     2401 gctctgtgca ttcctcgccg tcagcggcgg tgttcagggg tccggtgtac tgtggattta
     2461 ttcatttccc ctgttgtcta tttttctcct tggtatgaag tcgggaatcg cgctatctac
     2521 ggtgttgtta ttcatcgtta gcgcagcggt ctttattccc ggagtatctc ccatgaagtt
     2581 cgagacctcc tttgccctgc gtacggttgg tgtgtatatc ctggtactgg tgtgtaccat
     2641 cgtatatgaa cagaccaagc tggttaagga tcgatgggtt gcccgtctta ccaatgctct
     2701 gcaagtggaa cgcgatgaaa ttaccgccat gaaggacaac cttaaggtag gtttgttttt
     2761 gattaatcgc gattatatca ttcagcccca gtattcaaaa gcgcttgaaa cggttatggc
     2821 tgaaactgac ttgaatggaa aaaattttat tgatgtactt tca
//
LOCUS       DAOQXQ010000157         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37629_length_2861_cov_2.972915, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000157 DAOQXQ010000000
VERSION     DAOQXQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37629_length_2861_cov_2.972915"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKH81_03430"
     CDS             <1..513
                     /locus_tag="PKH81_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97118.1"
                     /translation="FMSVSSDGVTEWIPWKRLLAVETGSTVMVYLPASGKRRTYCVFH
                     EEKNAKAFRERLRERRMHHHLSDPATPFFVALGAFLEFAFFLSAFTGGELDTASSLAL
                     IAIFGKALPWAPPGLALTLWSHRIQDRAQDKKKRRQSLTVGYVLTGLGVLLNGALILF
                     VIRGIAFSVR"
     gene            complement(457..1269)
                     /locus_tag="PKH81_03435"
     CDS             complement(457..1269)
                     /locus_tag="PKH81_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNQ97119.1"
                     /translation="MSEKRKSALNADQITEYLQDVVKKIRTEEDPLLLNQYRRLFRSS
                     VPLTLRSYLAAYLLKQIDDGAFPGSFGSALRTGRSFRDKRDRSDRSDRSERSDRGNRG
                     EKAPRADKGARAEKTERASRERPSTENRPVERVVLPEELATTLFVSIGRNRRVFPRDL
                     IGLIMQNVEIDRDHIGDIRVLDNYSFVQVITEDADRVIEGLHEMEYRGRKLVVSYSKK
                     KEESTEQETDGYTEADSSTDDSFAQDDYSEQDTYLSDGESDTPDNEEDQSSV"
     gene            complement(1316..2245)
                     /locus_tag="PKH81_03440"
     CDS             complement(1316..2245)
                     /locus_tag="PKH81_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ97120.1"
                     /translation="MVKSNQMEFELTPEITDEIIFSMEDQAGIFLFDTCETRCVSSDA
                     ATQPNGETERYYAIPQWDSVSGFRMMDRFAAQLRNPVVREDLRVALSSGQGVFRKFKN
                     ILKEYPEVERLWFTYKEREMRSIVREWYNNLRDFWGLERIGEEPEETEELVREDFTFR
                     IIHGPVDTAISELIEDAERELLMDLPLGLHDALTLVISRLRPEISGMVTIVVDDMEGL
                     SVGAARSGPLPSDSLHSAALETIAVLPEFRGLGLGKELASRTVRYWQERLYRHLVCVA
                     PVIPQPFYTVLRRLGFENLGHLSVLNFTQRRDH"
     gene            2296..>2861
                     /gene="eno"
                     /locus_tag="PKH81_03445"
     CDS             2296..>2861
                     /gene="eno"
                     /locus_tag="PKH81_03445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968021.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ97121.1"
                     /translation="MSVIAYIDGREILDSRGNPTVEVDVYLEDGSRGRAAVPSGASTG
                     EHEALELRDADPSRYKGKGVLDAVENINTLIAEELDGADALDQAEIDSMLLTLDGTAN
                     KAKLGANAILGVSMATARAAADSLGIPLYRYLGGAHTLQLPVPMANIINGGQHSDNKI
                     DFQEFMIMPVGAPSLREAVRMTAEVFHTL"
BASE COUNT          674 a          748 c          718 g          721 t
ORIGIN      
        1 tttatgtccg tcagctcaga tggggtaacc gaatggatcc cctggaaacg acttcttgca
       61 gtggaaaccg gctctacggt tatggtgtat cttccggcaa gcggtaagcg tcgcacctac
      121 tgtgtatttc acgaagagaa aaatgccaaa gcgttccgcg agcgcctgag agagcggcgg
      181 atgcatcatc atctgtcgga tcccgccact cccttttttg tagctcttgg cgcctttttg
      241 gagtttgcct tttttctttc tgcctttacc gggggggaac ttgatacggc gagctctttg
      301 gccctgatcg ctatctttgg caaggccctt ccctgggcac caccaggact tgcgcttacc
      361 ttatggtcgc atcggataca ggatagggct caggacaaaa aaaaacgccg acagtcactg
      421 accgtcggct atgtgttaac aggattgggc gtccttttaa acggagctct gatcctcttc
      481 gttatccggg gtatcgcttt ctccgtccga taggtaggta tcctgctcgg aatagtcatc
      541 ctgggcaaag ctatcatcgg tggatgaatc tgcctctgta tatccgtcag tttcctgttc
      601 ggtcgattct tcctttttct tggaatagct gactacgagt tttcgtcccc gatattccat
      661 ctcgtgtaag ccttctatta cccgatcagc gtcctcggta attacttgga cgaatgagta
      721 gttatccaac acgcgaatat ctccgatatg atcgcggtca atctccacat tctgcataat
      781 caagccaata agatcacggg ggaacacacg ccgattgcgt ccaatgctca caaagagggt
      841 ggtcgccaat tcttcgggaa gtactacccg ctctaccgga cgattttctg tagaaggtcg
      901 ctcacggctt gcccgttctg tcttctccgc tcttgctccc ttgtcggccc ggggagcttt
      961 ttcaccacga tttccccgat cactgcgttc gctgcgatcg cttcgatcgc tgcgatctcg
     1021 tttatcccga aatgaacgac ccgttcgaag ggctgaacca aatgagccag ggaaggctcc
     1081 atcatcaatc tgcttgagca ggtatgccgc aagatatgac cgcaatgtta agggtactga
     1141 cgagcggaat aaacggcgat actgattgag caggagggga tcttcttccg tcctgatctt
     1201 tttaacaacg tcctgaagat attcggtaat ctgatctgca ttcagggcgc tttttctctt
     1261 ttctgacacg ataacattct cctgtagtca ataatgccat aattatggcg cttactcaat
     1321 ggtctcgccg ctgggtaaag ttcagaaccg acaggtgtcc caggttctcg aaccccagtc
     1381 ggcgtaatac ggtataaaag ggctgcggga taaccggggc gacacagacc agatggcgat
     1441 agagtctttc ctgccagtag cgcaccgtcc gggaagcgag ctctttaccc aagccgagtc
     1501 ctctaaactc gggtaataca gcgatagtct ccaacgcggc gctatggaga ctatccgatg
     1561 gcaggggtcc cgagcgggct gcgccaaccg acaggccttc catatcatcc acgacaatcg
     1621 ttaccattcc cgatatctca ggacgaagcc gactaatcac cagagtaagg gcatcatgaa
     1681 gtcccagggg aagatccatt aggagctccc gttcggcatc ttctatcaat tcacttatcg
     1741 cagtatcaac gggcccgtga ataatccgga aggtaaaatc ctcccgaaca agctcttcgg
     1801 tttcttcagg ctcttctcca attcgctcca aaccccaaaa atcccgcaag ttattgtacc
     1861 attcacgaac aatggagcgc atttcccgct ctttataggt aaaccacagt cgttctacct
     1921 cggggtattc ttttagaatg ttcttaaact tgcgaaatac accttgaccg gaggaaaggg
     1981 cgacccgtaa gtcttcacga accacggggt tgcgcaattg ggcggcaaaa cggtccatca
     2041 tcctgaatcc gcttaccgaa tcccactggg gtatggcgta atatcgttcg gtttccccat
     2101 taggctgggt ggcagcatca gaactgacgc aacgggtttc gcaggtgtca aacaggaaga
     2161 tgcctgcctg gtcttccatg gaaaaaataa tctcgtcggt tatctcaggt gttaactcaa
     2221 attccatttg gtttgatttt accacggata ccccgtatta taaaggttga ccactatggt
     2281 cagggaggaa tgtatatgag tgttattgcg tatatcgacg gtcgggaaat ccttgattca
     2341 agaggaaatc ccacggtaga ggttgacgta tatcttgaag atggaagcag aggccgcgcg
     2401 gctgtgcctt ccggagcctc cacaggagaa catgaagcat tggagctcag ggacgcagat
     2461 ccttcacgat acaagggaaa gggtgtattg gatgcagtag agaacatcaa tacactgatt
     2521 gccgaggaac ttgacggggc cgacgccctg gatcaggcag agattgacag catgctgttg
     2581 acccttgacg ggacggctaa caaggcaaaa ctcggcgcta acgcgatcct tggtgtttcc
     2641 atggcaactg cccgggcagc ggcagattcg ctgggaattc cactctatcg ctatttaggc
     2701 ggcgcccaca ccctgcagtt accggttcct atggctaata ttatcaatgg cggacagcat
     2761 tcagacaaca agatagactt tcaggagttt atgatcatgc ccgtaggtgc gccaagcctt
     2821 cgtgaggcag tgcgcatgac agccgaggtg tttcataccc t
//
LOCUS       DAOQXQ010000158         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37645_length_2860_cov_3.428164, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000158 DAOQXQ010000000
VERSION     DAOQXQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37645_length_2860_cov_3.428164"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="tilS"
                     /locus_tag="PKH81_03450"
     CDS             <1..874
                     /gene="tilS"
                     /locus_tag="PKH81_03450"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNQ97122.1"
                     /translation="LQGSGGASLRGIASIRGRYLRPLLGVSRGHIEAWLTDTGTPWRE
                     DSSNQDSRFYRNRVRNTLIPLLRAEFPGWERGLDSLSQKAAIDEDYIREEPLPAWTVQ
                     ANGLSCDRASFVNLHPARAIRFLHQGLCGLSIPNRVSFRFVSRILREGKTLDEGRSIQ
                     GSALSFSRRGEFLFLRPYIVKNEKSGYLVYIRGSAQVQLPYGIMNVCLCDGSVCIDGR
                     FGPFSVPLIVRSRRPADRVQTADGTSKTIKKLLNSWGVPEDVRDMLPVIEQAGIIRAV
                     LGSPLGYQDWLVRS"
     gene            882..2750
                     /gene="ftsH"
                     /locus_tag="PKH81_03455"
     CDS             882..2750
                     /gene="ftsH"
                     /locus_tag="PKH81_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNQ97123.1"
                     /translation="MAQNDMDGKPSGSRPAGKSGRFALVFLLVSLILFVFVFFSGFGQ
                     PAGNEMSYTEFKARVSQGQVDSVKIIDSRKIRGVLRDVRNPDGFFYTFIPYSDPSLME
                     LLTQHNVRITGGEKPVSPWLYVLQFGPSVLIIVFLVLMMRQSQGQGNKAFQFGKSQAH
                     RYEGTKKITFADVAGQLEAKHELEEVVEFLKNPQKFIKMGARIPKGVLLVGMPGTGKT
                     MLARAVAGEAGVAYFNISGSDFVEMFVGVGASRVRDLFEQGRKNAPCIIFIDELDAVG
                     RTRGAGYGGGHDEREQTLNQMLVEMDGFETKDGVIILAATNRPDVLDPALLRPGRFDR
                     QVVVAMPDLREREAILKIHAARIPLDSSVDLQRFARATPGMSGADLANLVNEAALHAA
                     RKDKATVESSDFEEARDKVLMGVARHSMVMPERDRKMTAVHESGHALLHYFLENADPL
                     HKVTIIPRGQALGLAISLPEQDSYSRTKGWLSDRLVICYGGYVAETLVYGETTTGTKA
                     DLQQATEIARRMVCEYGMSDHVGAVTYGQEDEPIFIGKEIARHKDYSEQTAQAIDQSV
                     RTILDNARERALAIIGEHRVELDALTEALLRQETLDDCEIRQLLSIERTGDAQPGQ"
BASE COUNT          661 a          654 c          794 g          751 t
ORIGIN      
        1 tctacagggc tctggagggg cttctctccg cggtattgca tcaatacgcg gcaggtacct
       61 caggcccctt ctaggggttt ctcgcgggca tatagaggca tggctaactg atacgggcac
      121 cccctggagg gaagattcca gtaaccagga ttcacggttt tatcgaaatc gggtgcgtaa
      181 caccctgatt cctctgttac gtgccgaatt tcccggatgg gagcggggcc ttgacagttt
      241 gtctcaaaag gcggctattg acgaagacta cattcgcgaa gagcccttgc ctgcatggac
      301 agtccaggca aacggtcttt cttgcgatcg tgcctccttt gtcaacctgc accccgcacg
      361 agctatacgg ttccttcatc aggggctctg tggtctgtcg attccaaatc gggtctcctt
      421 ccggtttgta tcccgtattc ttcgcgaggg aaaaacgctg gacgagggaa ggtccattca
      481 gggctctgcc ctttcatttt ctcgccgtgg cgaattcctt tttttaagac cttatattgt
      541 aaagaatgaa aaaagcgggt atcttgtgta tattcgtggc agcgcacagg tacagcttcc
      601 gtatggaata atgaatgtgt gcctctgtga tggatcggtt tgtattgatg gtcgttttgg
      661 acctttcagt gttccactga ttgtacgttc acgacgtccc gcagaccgtg tacaaactgc
      721 cgacggtaca agtaaaacga tcaaaaagtt gttgaattcc tggggtgtac cagaagacgt
      781 gcgtgatatg cttcccgtca ttgaacaggc gggaatcata cgggccgtac tgggtagtcc
      841 gcttggatat caagactggc tggtccggag ttgaggaaat aatggcacaa aacgatatgg
      901 acggtaaacc ttccggatca cgacctgccg gcaagagcgg tcgtttcgcc ctggtattct
      961 tgttagtgtc cctgatctta tttgtctttg tctttttttc cgggtttggt cagccagccg
     1021 gaaacgagat gtcttatacc gagtttaagg ctcgggtgtc gcaggggcag gttgattctg
     1081 tcaagattat tgattccaga aaaattcggg gagtacttcg tgatgttcga aaccctgacg
     1141 gtttttttta taccttcatc ccgtactcag atccttcgtt gatggaactt ctgacacagc
     1201 ataatgtacg catcactggt ggtgaaaagc cggtgtcgcc ctggctctat gtcctgcagt
     1261 ttggaccttc ggttcttatc attgtgttcc tggtgcttat gatgcgccaa tcccagggac
     1321 agggaaataa ggcctttcaa ttcgggaaaa gtcaggctca tcgatatgag ggaacaaaaa
     1381 aaattacctt tgccgatgtg gcaggccaat tggaagccaa gcatgagctt gaggaagtgg
     1441 tagaattcct gaaaaacccg cagaagttta taaagatggg cgcccgcatt ccaaagggag
     1501 ttcttctggt tggtatgccg ggtaccggaa aaaccatgct tgctcgcgct gttgccggag
     1561 aagccggggt tgcctatttt aatatttcgg gtagcgattt tgtagagatg tttgtgggtg
     1621 ttggcgcaag tcgtgttcgg gacctgtttg aacaggggcg gaaaaacgcg ccctgcatca
     1681 tttttattga cgaacttgac gcagtaggtc gcactcgggg agccggctat ggcggcggtc
     1741 atgatgagcg agagcaaacc ttgaatcaaa tgcttgtgga gatggatggc tttgaaacaa
     1801 aggacggcgt cattattctg gccgctacaa accgccctga tgttctcgat cctgcattac
     1861 tcaggcctgg tcgttttgac cgtcaggttg tagttgccat gcccgatctt cgggaacgcg
     1921 aggcaatttt aaaaattcac gccgcacgga ttcccttaga cagttccgtg gatctgcagc
     1981 gttttgcacg ggcaactccc gggatgagcg gggctgattt ggctaatctt gtaaacgaag
     2041 cggctcttca tgcagcccgt aaggataagg caacagtaga atcgtctgat tttgaagagg
     2101 cacgcgataa ggttcttatg ggcgttgccc ggcattccat ggtgatgccc gaacgagatc
     2161 gaaaaatgac tgcggtccat gaatcgggac acgcactgct acactatttt ttggaaaatg
     2221 ctgacccctt gcacaaggtg accatcattc ctcgtggaca ggcccttggg cttgctatct
     2281 cgcttcccga acaggattcg tactcccgca ccaaggggtg gttaagcgat cgccttgtta
     2341 tctgttatgg cgggtatgta gccgaaaccc tggtgtatgg tgaaaccaca acaggtacca
     2401 aggcggattt acaacaagca accgaaattg ctcggcgtat ggtgtgcgag tatggtatga
     2461 gcgatcatgt tggagcggta acctatgggc aagaggatga acctattttt attggtaaag
     2521 aaattgcccg tcataaggac tattctgaac agactgctca agcgattgat cagtctgttc
     2581 gtacgattct tgataacgcc agagaaaggg ctcttgccat cattggcgaa caccgggttg
     2641 aacttgatgc gctgacagaa gccttgcttc ggcaggaaac ccttgatgat tgcgagatac
     2701 gacaattgct ttcgatagag aggaccgggg acgcacagcc gggccaataa ctgaggtact
     2761 ctacggggag gtactgatat ggatctagag cacaccaaac gctcaggcgg atcgggattg
     2821 aggatgttgt ggacagcctc gctcatggta tgcttgttgc
//
LOCUS       DAOQXQ010000159         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37652_length_2860_cov_3.110517, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000159 DAOQXQ010000000
VERSION     DAOQXQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37652_length_2860_cov_3.110517"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PKH81_03460"
     CDS             complement(<1..1165)
                     /locus_tag="PKH81_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ97124.1"
                     /translation="MKHPGLFCILALALAALPLVSLPAEDQDGGSEISQSTTGTLRTQ
                     SRFARDGNTTSTLIINTDIPKTDIYLNGVYEGQTPKTFSRITAGIWRVSLTKNGYYSE
                     SFLIRIRPGETKELLMELVPITGSVLVQNAPPNAVYELDGQRYTSPRITATEGSHTLT
                     VKAFGYTEKTHTIVVFRERDTLVDGSLDTAPFRLDSFRARKTRFNPDNPGVLDFTFMV
                     TAPGSGTLKIQDEEGRLITDIPLQTFATWRQTVSWDGMTDAGSASDGLYTARLVVYSQ
                     GTEESQTTEATSTFYIDHSIVHPWSWAFRGIGAIGAVASPSIHPEGEGAFQIDMHWYN
                     NTAHIGFSALRSFYHNLELSGRIAVTVQEDEQAKLAGATHITVALTPGTLKSAC"
     gene            complement(1227..2525)
                     /locus_tag="PKH81_03465"
     CDS             complement(1227..2525)
                     /locus_tag="PKH81_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNQ97125.1"
                     /translation="MEKLFKLKAHGTTVRTEVLAGITTFLTMAYILAVNPGILGDAGM
                     DRSAVFTATALASAVASIVMAIAANLPIALAPGMGLNAYFTYGVVLGMGYSWQVALSA
                     VFLEGILFILLSVVNVREAIVNSVPLNLKRAVAVGIGIFITLIGMRGAGIIIPSPATT
                     VAMGSVTQGTALLALIGLVITAILYALNVHGAILIGILITTAIGIPMGITAPAGGWEN
                     WKLFSAPSAPLFMQFDFSNILTIKFLVVFFSFLFVDIFDTVGTLVGVTQQAGLTDKDG
                     NVPRVKQALLADAVGTSFGAMVGTSTVTSYVESTAGVAAGGRTGLTSLTTAGMFLLAL
                     FLSPLFLIIPAAATAPALIIVGFLMMRPVIGIDFTDPTEGIPAFLTIVMMPFCYSIAE
                     GIVFGVLSYVILKACTGKYKQIPLVTWVIFAIFILRFILH"
     gene            complement(2620..>2860)
                     /locus_tag="PKH81_03470"
     CDS             complement(2620..>2860)
                     /locus_tag="PKH81_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNQ97126.1"
                     /translation="RTIVHPREVFADPLSDRAAAVIVCHNHPSGQLEPSEEDRSITKR
                     LTGAADILGITLLDHLIISPKGGYFSFVEAGIPLS"
BASE COUNT          752 a          670 c          768 g          670 t
ORIGIN      
        1 cgcaggcgct tttaagggta ccgggagtta aggctactgt tatatgcgtg gctcccgcga
       61 gtttcgcttg ctcatcttct tgaacggtta cggcgattcg gccgcttaat tccaggttgt
      121 gatagaacga tcgcagggcg gaaaatccta tatgcgcggt attgttatac cagtgcatgt
      181 caatctgaaa ggccccttcg ccttcaggat ggattgatgg ggacgcgacc gcaccaatag
      241 ctcctatccc gcgaaaggcc caggaccagg gatgtacaat agagtgatca atatagaaag
      301 tggatgttgc ttccgttgtt tgactttctt cggttccctg tgaatatact accaggcgag
      361 ccgtatatag gccatcgctc gcgctccccg catctgtcat gccatcccag gatactgtct
      421 gacgccaggt ggcaaaggtt tggagcggta tgtccgtgat gagccttccc tcttcgtcct
      481 ggatcttgag cgtaccgctc cccggggcgg taaccataaa agtaaaatca agaactccgg
      541 gattatcagg attaaatcgg gttttgcgcg ccctgaaaga atccagacgg aatggagcgg
      601 tatcaaggct accgtctacc agggtgtctc gctcgcgaaa cactacgatg gtgtgcgttt
      661 tttcagtata tccaaaggcc tttaccgtca aggtatggga accctctgta gcggtaatac
      721 gcggcgaggt atatcgctgt ccatcaagct cgtacacggc gttaggtggc gcgttttgga
      781 caagtactga accggtaatc gggacgagct ccatcagtag ctcttttgtt tcgccaggtc
      841 gtatacggat taaaaaactt tcgctgtaat atccgttttt tgtaagggac actcgccaaa
      901 ttccggcggt aatcctggaa aaggtttttg gggtttgtcc ttcatagaca ccattcaggt
      961 agatatcggt tttggggata tcggtattga tgattaaggt gcttgtagta ttaccatcgc
     1021 gggcaaaacg agattgggtg cgaagggttc cggtcgtcga ctgcgagatc tcgctcccgc
     1081 catcctgatc ttcagctggt aaggatacaa gggggagcgc ggcaagggcg agcgcgagta
     1141 tacaaaaaag ccccgggtgt ttcatgtatc catgataacc cggggactct aaaaagaaaa
     1201 gcctgacaag tcaggcttac tcaagatcag tgaagaataa aacggagtat aaagattgca
     1261 aagattaccc aggtaacaag cgggatttgc ttgtatttac cggtacaggc cttaaggata
     1321 acatatgaaa gtacgccaaa gacgattcct tctgcgatgc tgtaacaaaa cggcatcata
     1381 acaatggtaa ggaatgccgg aataccctcg gtaggatcgg taaagtcgat gccgataacc
     1441 gggcgcatca ttaaaaagcc cacaatgata agagccggcg ctgttgcggc tgcgggaatg
     1501 ataaggaaga ggggtgaaag aaacagggca agaaggaaca tacctgctgt agtcaagctt
     1561 gttaagccgg ttcgtccgcc tgcggccaca ccggcggtac tttcaacata gcttgttact
     1621 gtcgatgttc ctaccatggc gccaaaggat gtaccaacgg catcggcgag gagggcttgc
     1681 tttactcgag gtacatttcc atccttgtcg gtaagtcctg cttgctgggt tactccaacc
     1741 agggttccca ctgtgtcaaa gatatccaca aaaaggaagg aaaagaacac aaccaggaat
     1801 ttaatggtca ggatattgga aaaatcgaat tgcataaaca agggagcgga tggtgcgcta
     1861 aagagtttcc agttctccca tcccccggcc ggagcggtga ttcccatggg aataccaatc
     1921 gctgtggtga taagaatacc gatcaatata gccccgtgta cgttaagagc gtataaaatg
     1981 gcggtgataa ccagaccgat caaggcgagt aaggccgtcc cctgggttac tgaacccatg
     2041 gctaccgtag ttgccggact aggaatgatg atacccgcac cccgcatacc gataagagtg
     2101 ataaagatac cgataccaac ggcaactgcc cgcttcaggt taagtggcac agaattgaca
     2161 attgcctctc tgacatttac caccgaaagc aggataaaca ggatgccttc cagaaaaaca
     2221 gccgagaggg cgacttgcca gctatacccc ataccaagga caacaccata ggtaaaatag
     2281 gcattgagtc ccattccggg tgccaacgcg atggggagat tggcggcaat ggccattaca
     2341 attgacgcta ccgcagaggc aagggctgtt gcagtaaata cggcgcttct atccataccc
     2401 gcatcgccga gaatacccgg atttacggcc aggatgtagg ccatggtcaa gaaggtggtg
     2461 ataccggcaa gaacttctgt tcttacggtt gtcccgtgcg ctttcagttt aaaaagcttt
     2521 tccatgatgg tgtgtgctcc tcttgtgaat ttgtgtgtac agagaatacc caagagtacc
     2581 accgtttttt tcaccctaca agcgggtttt tacgatatat caggaaagcg ggataccagc
     2641 ttctacaaaa ctgaagtacc cgcctttggg actgataatc aaatggtcaa ggagggtaat
     2701 gcccagaatg tctgcggctc cggtcagacg tttggtaata gagcggtcct cttcggaggg
     2761 ttcaagttgt ccggaaggat gattatggca gacaattacc gccgccgcac ggtcggaaag
     2821 ggggtccgca aaaacctccc gtggatggac gatggttctg
//
LOCUS       DAOQXQ010000160         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_37759_length_2855_cov_2.862500, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000160 DAOQXQ010000000
VERSION     DAOQXQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37759_length_2855_cov_2.862500"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /gene="fliD"
                     /locus_tag="PKH81_03475"
     CDS             <1..649
                     /gene="fliD"
                     /locus_tag="PKH81_03475"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNQ97127.1"
                     /translation="NYNRLMAELNILTQKRPEIIQEIGYFTPDESKAAEEKLGMMLGD
                     TTLNGIKASLQRITTNLYVSSDTSSIRSLAELGISTKSGTGGGLDSSRLRGYLEIDEK
                     KLDAALDARMNEVKTLFGFDSDGDLIIDNGLAHSMDNALAPYVQTGGIFATRTSGLDN
                     RISTTEKKITRLDEQLEAKERELKIKYGQMEGTLNSLQQQSNSIQNFNNQNTRNQ"
     gene            661..1254
                     /locus_tag="PKH81_03480"
     CDS             661..1254
                     /locus_tag="PKH81_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97128.1"
                     /translation="MKILLNGKNLDYTLDTEETVGEVLGSVESECEKAGMTVTSVRVD
                     GHTIDAAALDALFRKSPDEVTSIELETLNGESIIKALQDLGKRFTDMIPVIEDIPVLL
                     QTGKDLTVMETIHTFSVDIEQFYRLLPLFPLTGMDATTITAGSVPLGNYPGELAPLLS
                     QLLDALKQRDTVLVGDIAEYELAPRLSELGLALQDLS"
     gene            1265..2410
                     /locus_tag="PKH81_03485"
     CDS             1265..2410
                     /locus_tag="PKH81_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNQ97129.1"
                     /translation="MIEKILNQVLPYSTSTDPRYLVVILIVIGIITVAAIINRQQEAG
                     KKKRSQSRSILGEVAKNNLVFVKNRKFKQEIREITSLCSLDKNQSEFLSSLCATNHVE
                     HPLQILKNTKFLEEIFSRAFQKLEIPSQKTKETELHKTILFTLREALAKVQKSEKGIS
                     STRSLDNGWTLTIVTPQDEQYETTLLENTSKGMICPVPRDAFSNELRLPLWTKVGLFF
                     SIHTGQSYRCDARIIHYESSPGQTILMLSHTDSLKPLPNRQHNRKAFSCDCSITPVSV
                     ANIVNGKHTEHRFYPSGKDNPGTMNDISAGGCSIISPYTPAQNQYIQIQCSLDSKNAD
                     SMIGKVVRISSGDSEANAILHIQFAKMPRATMNRIFALIFNYGELHK"
     gene            2407..2667
                     /locus_tag="PKH81_03490"
     CDS             2407..2667
                     /locus_tag="PKH81_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97130.1"
                     /translation="MTVLAIDDIVRKENFIYYRREFSGKALYDLPGRKHTGQIEFVIE
                     TAPTGKKEIQIKLVDSVDYPLLPVMSTLKQYIMELDRDGRLP"
     gene            2713..>2855
                     /locus_tag="PKH81_03495"
     CDS             2713..>2855
                     /locus_tag="PKH81_03495"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNQ97131.1"
                     /translation="MILTTTTEEQTISLGKKIGLFLKDGDVIALQGTLAAGKTTLTKG
                     IALG"
BASE COUNT          888 a          661 c          588 g          718 t
ORIGIN      
        1 caactataat cggcttatgg cggagttaaa tatcctgact caaaaaagac cggaaattat
       61 ccaggagatt ggctacttta ccccggatga aagcaaggcc gcagaagaaa agctgggaat
      121 gatgcttggc gatactaccc tgaacgggat taaagcaagc cttcagcgaa ttaccaccaa
      181 tttgtatgtt tcatcagaca ccagttcaat tagatccctt gccgaactcg gtatttctac
      241 caaatcgggt actggtggcg gacttgattc atccaggttg cgcggatacc tggaaataga
      301 cgaaaagaag ctcgatgcag ccctggatgc ccggatgaat gaggttaaaa ccctgtttgg
      361 ttttgactca gacggtgatc tgataatcga caacggcctg gctcattcca tggacaatgc
      421 ccttgccccc tatgttcaaa ctggaggaat tttcgcaacc cgcacttctg gtttggacaa
      481 tcgaatatca actaccgaaa aaaagattac caggctcgat gaacagcttg aagctaaaga
      541 gcgagaacta aaaatcaagt atggccaaat ggaagggacc cttaacagcc ttcagcaaca
      601 gtcaaactcg attcaaaact ttaacaatca aaacacccgg aaccaatagg agacgaatcg
      661 atgaaaattc tgttaaatgg caaaaacctg gactatacac ttgataccga agaaaccgtt
      721 ggcgaagttc tgggttcagt tgaatcggaa tgtgaaaaag cgggaatgac ggttacctct
      781 gtacgagtgg atgggcatac tatagacgca gcagcccttg atgcgctttt tcgcaagagt
      841 cccgacgaag taacctccat tgagttggaa acgctcaatg gtgaatctat tatcaaagcc
      901 ttacaagatc ttggcaagcg atttaccgac atgattcctg ttatcgaaga tatcccggta
      961 ttattgcaaa caggaaagga tcttacggta atggaaacca tccatacttt ttctgtggat
     1021 attgagcaat tttatcgcct tcttccccta ttccccctta ccggtatgga tgcaactacc
     1081 attacagctg gttcggttcc tctgggtaat tatcctggtg aactcgctcc gttactttcc
     1141 caattattgg acgctcttaa gcagagagac accgttctgg ttggagacat tgccgaatac
     1201 gagctcgcac cacgattaag cgaacttgga ttggcgcttc aggacttatc ctgaaggggc
     1261 acaagtgatt gaaaaaatcc tgaatcaggt gttgccgtac agcacctcaa cggatccacg
     1321 gtaccttgtg gtaattctta ttgttattgg cattattacc gttgcggcga ttataaaccg
     1381 gcaacaagaa gcaggaaaaa aaaagagaag tcaatcccgc tctatattag gcgaagtagc
     1441 taaaaacaac ctggtatttg ttaaaaaccg caagttcaaa caggaaatac gggaaatcac
     1501 ttcgctctgc tccctcgata aaaatcaaag cgagtttctc tcttccttgt gtgctacaaa
     1561 tcacgttgag catcccctcc aaattttaaa aaacaccaaa ttcctggaag aaatatttag
     1621 tagagccttt caaaaattgg aaatcccgtc tcaaaaaaca aaagaaacag aattacataa
     1681 aacaatcctt tttaccctgc gggaagccct agccaaggtt caaaaatcgg aaaaaggaat
     1741 atcgtctacc cgctcccttg ataatgggtg gacactaaca attgtgaccc cgcaagatga
     1801 gcaatacgag acgacattac tggaaaacac ctcaaagggg atgatttgtc ccgtaccccg
     1861 agacgctttt tctaacgaat tgcgtcttcc cttatggact aaggttggtc tgtttttttc
     1921 catacacaca ggccagtcct atcgatgtga cgcgagaata attcattatg aatcatcgcc
     1981 tggacaaaca atcttgatgc tctctcatac tgactcactt aaacccttac caaacagaca
     2041 acataaccgt aaagccttta gctgcgattg ctcgataaca ccggtatctg tggctaatat
     2101 cgttaacggc aaacacaccg aacatcgttt ttacccttca ggtaaagaca atccgggaac
     2161 catgaatgat atctccgccg gtgggtgtag cattataagc ccgtacacac ctgcacaaaa
     2221 tcaatatatt cagatacaat gttccctgga cagtaaaaat gcggattcaa tgattggcaa
     2281 ggttgtccgc attagttcgg gagattcaga agcaaatgct atcttgcata tacagtttgc
     2341 caaaatgccc cgagctacta tgaacagaat ttttgcatta atattcaact atggagagct
     2401 tcataagtga cagtccttgc aattgacgac attgtacgaa aagaaaactt tatttattac
     2461 cgcagagagt tttccggtaa agcgctttat gatcttcctg gcaggaaaca caccggccag
     2521 atagaattcg ttatagaaac agccccaacc ggtaaaaaag agattcaaat caagctggtt
     2581 gattccgtag attatcccct cctccctgta atgagtaccc tgaagcaata cattatggaa
     2641 ctcgatcgcg acggaaggtt accgtaagct tacatattga ccctttcggg tctatacagt
     2701 accttccatc tcatgatcct gaccactacc accgaagaac aaactatatc ccttggtaaa
     2761 aaaatcgggc tttttcttaa ggatggagat gtaatcgcct tacaaggaac cctggctgcc
     2821 ggtaaaacaa cacttaccaa gggaatcgcc ctggg
//
LOCUS       DAOQXQ010000161         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_38154_length_2835_cov_2.739928, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000161 DAOQXQ010000000
VERSION     DAOQXQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38154_length_2835_cov_2.739928"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="rseP"
                     /locus_tag="PKH81_03500"
     CDS             <1..1256
                     /gene="rseP"
                     /locus_tag="PKH81_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNQ97132.1"
                     /translation="ARLSGVTVESFSLGWGPLLLKFKRGDIEYRFSVFFMGGYCGMKG
                     QDAYREALDANLSEIPREKGSFYGAHPLKRIAISFAGPFSNLLFSVLVFAFVSAVGYT
                     YQTWENRIIPVPESIAGYETAAIKAGLLPGDRLISMDGIPIAHFADLQKTTSQRAGKT
                     VLVNYERDGVQYSTTVVIEMDKKTGAGKIGVLPWIPLIISTVKDGSAASSAGVRPGDR
                     IIAIDGTAVSHYFELTDALSSKPEQAIFTIQRNDIEIRIPIVLLYKPDTGTVETGMDF
                     ERQTITIAGTGLIQSMEQGIQETARTLYLTVKSLGLLFKGVDMSEAVSGPVRITLMLG
                     EVAQSSLIAVAELLAIICVSLFIMNLLPIPILDGGIIFFSLIELIRRKQLRPKTLYRF
                     QFVGIAFIVFLFIFALFGDISYLIK"
     gene            1281..1904
                     /locus_tag="PKH81_03505"
     CDS             1281..1904
                     /locus_tag="PKH81_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNQ97133.1"
                     /translation="MRKVTCPCDQTFSLDIPETINLDEQPQVISSILDGSFLSCVCPR
                     CGSSLNLDLPTRVEWPSKKAIIHLVPEVERISVMHGGRTVAEGELVVVGYSELADRIA
                     VLKEELDILAIESLKIRLLEKARESNPQATAQLVFEKKTPSGDLEFHLHGLREGEVAI
                     TVVPYRVYEMVLQSLKDNPDSEEYTALINGPYISAKNILLEDGDLNA"
     gene            1897..>2835
                     /locus_tag="PKH81_03510"
     CDS             1897..>2835
                     /locus_tag="PKH81_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNQ97134.1"
                     /translation="MPKSLPVTLFCILLSVCAPLVFAAEPSLVSEIAGYSGSDQAVVR
                     SGLHQHRGSVLALARQGTSDAFFSAGEDGFISLHDPLGNSHIWQISDMPIKMIAVHPD
                     GNLIASYETDGFSVHRLSLWSWKDKKRLYAKRFREAITSVSWSAKGTYVMIGNTSLDG
                     ITVLTGETGKPKKIFDTSPGPVSLSHTGAKETSVITFGPLGAIRYTEISNGKQRAVYQ
                     GPAHISSPSLFDNNRRITGFSGTHVLSIDATSGKVVQEFPTTKAIMATGSFDNQPMWF
                     SPSGEDWQLCREDKTSAPFATTDYEAITTALAMESKI"
BASE COUNT          802 a          602 c          636 g          795 t
ORIGIN      
        1 ctgcacgttt gagcggagtt accgttgaaa gtttctccct gggatggggg cccctcctgt
       61 taaaattcaa acgaggcgac attgaatatc gtttttctgt tttttttatg gggggatact
      121 gcggaatgaa aggacaagat gcatatcggg aagcccttga tgcaaacctt tcggaaatcc
      181 cccgcgaaaa aggaagcttt tatggggccc accccttaaa aagaatcgca atttcatttg
      241 caggaccctt ctctaattta ttgttttctg ttcttgtgtt tgcctttgtt agcgcggtag
      301 gctataccta ccaaacctgg gaaaacagga ttatccctgt acctgaatcg attgccggat
      361 atgagactgc agcaataaag gcaggacttc ttccaggaga tcgtcttatc tccatggatg
      421 gcattcccat tgcacatttt gcagaccttc aaaagacaac ttcccagagg gcaggaaaaa
      481 ccgtccttgt aaactatgaa cgcgatggcg tccaatactc aaccactgtg gttatagaaa
      541 tggacaagaa aaccggcgcg ggaaagatag gcgtattacc ttggattcct ttgataatat
      601 caacggttaa agatgggtcc gctgcatcgt cagcaggagt tcgtcctgga gatcgtatta
      661 ttgcaattga tggaacagcc gtctcgcatt attttgagct aaccgatgcg ctaagctcaa
      721 aaccagagca ggctatattt accattcaac gtaacgacat agaaattcgc atacctattg
      781 ttttacttta taaacccgac acaggcacgg tagaaaccgg aatggatttt gagcgccaaa
      841 ccattactat tgcaggaaca gggcttatcc agagcatgga acagggtata caagaaacgg
      901 cgagaaccct ctatcttacc gtaaaaagcc ttggattgtt attcaagggc gttgatatga
      961 gtgaagcagt ttctggtccc gttcgaatta ccttaatgct tggagaggta gcccagagca
     1021 gtctcatcgc tgttgcagaa ctattggcaa ttatctgtgt gtcgcttttt attatgaacc
     1081 tgttgcctat tcctatactg gacggcggaa taattttctt tagtcttata gaacttatac
     1141 gacgcaagca gctcagacct aaaaccttgt atcgatttca gtttgtcgga atagctttta
     1201 tagtgtttct ttttattttt gccttatttg gcgatatatc atatttaatt aaataggcgc
     1261 tcttccagga ggattccaag atgagaaaag taacttgtcc ctgtgatcaa actttttcgc
     1321 tcgacattcc cgaaaccatc aatcttgatg aacaacccca agtaatctcc agtatactcg
     1381 acgggtcatt tttaagctgt gtttgtccca gatgcggatc ttcgctaaat cttgatttac
     1441 cgaccagggt tgaatggcct tcaaaaaaag caataatcca cctggttccg gaggttgagc
     1501 gaatatcggt gatgcacgga gggagaacag ttgcggaagg agaattagtt gttgtcggtt
     1561 attcagaact cgcggaccgc attgctgtac tcaaggaaga acttgatatc ctggccattg
     1621 aatcattaaa aatacgatta ctggaaaaag ccagagaatc gaatccccag gctacagcac
     1681 aattggtatt tgaaaagaaa acaccgtcag gggatcttga atttcatctt catggactta
     1741 gagagggaga ggtcgctatc acagtggtgc cctatcgtgt ttacgaaatg gtacttcagt
     1801 cgctaaagga taatcctgat tcagaggaat acacagccct gatcaatgga ccatatatct
     1861 ctgcaaagaa tattctttta gaggatggtg atcttaatgc ctaaaagctt gcccgttaca
     1921 ttattttgta tactccttag tgtgtgcgct ccgttagtgt tcgcggcgga accatctctt
     1981 gtttcggaga ttgcaggata ttcaggaagc gatcaagctg tggtaaggtc tggattgcat
     2041 caacaccgag gtagtgtgtt agcccttgcc cggcaaggca cctcagatgc ttttttttca
     2101 gcaggtgaag acggttttat ctctttacat gatcctttgg ggaactccca tatctggcaa
     2161 atatcagata tgccaattaa gatgatcgca gttcatccgg atggaaatct tatagcatcc
     2221 tatgaaaccg acggatttag cgtgcatcgg ctatcactgt ggtcatggaa agataaaaag
     2281 cgactatacg caaaacgatt tcgcgaagca attacttcgg tatcctggtc tgcaaaggga
     2341 acctatgtaa tgatcggcaa tacctccctt gacgggatta ctgtattgac tggagagacc
     2401 ggtaaaccaa aaaaaatatt tgacacttct cccggaccgg taagtttaag tcataccggt
     2461 gctaaagaaa caagtgttat cacctttggt cctcttggag ctatacgata cacagagatt
     2521 tctaatggta aacagagggc tgtgtatcaa gggcctgctc atatcagctc accatcactc
     2581 tttgataaca atagacgaat cacgggtttt tccggaactc atgtactttc aattgatgca
     2641 acatcaggaa aggtagtaca agaattcccc actacaaaag ctatcatggc cactggttcc
     2701 tttgacaatc aacctatgtg gtttagtcct agtggggagg actggcaatt atgtagagag
     2761 gataaaacct ctgcaccctt tgccaccact gactatgagg cgataacaac agctcttgct
     2821 atggaaagta aaatt
//
LOCUS       DAOQXQ010000162         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_38469_length_2820_cov_3.448101, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000162 DAOQXQ010000000
VERSION     DAOQXQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38469_length_2820_cov_3.448101"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1481)
                     /locus_tag="PKH81_03515"
     CDS             complement(63..1481)
                     /locus_tag="PKH81_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNQ97135.1"
                     /translation="MADDKSTENQENTILYIRQGDPKDGYAEITVHGGALVATATFYP
                     PATGGSFLTYGLVSQRLEENGIIAGILHDAIQESILAANSSHKPVKEVTIAQSTPPVT
                     GIPEHYLIRQDLLQKPPKIDSEAEKIDWHELSAFTIVHAKDPIAKFIPEVPGEPGKNI
                     YGEEVPYPVQTLPVYTAGKNVISHEKGFFAGKSGRLSIDSKGVISIEDVLLLKKGVDF
                     NTGNIAFPGDVILQGKIADGFRVYSGGSIISSEVLDVTTEVVCKKDMSVQAGIEGKKV
                     GALRIGGNLTAKYIQNCRVAVRGDILVSGSIIQSTVYTMGMIKMGDTGKLVGCECIVI
                     GGVQALDIGSPRGAKTYLRCGTDFIVQQELDRANEQLKHIAVNLMKAEEMIKDEPSDK
                     IRAYIQSLIEKRSEITAKIPTFLPRIDTNDQAYVEVRGSIYPGVEIEICHVPYTVPKI
                     MKQVVFRLDKTRGAIVNEPYKK"
     gene            1585..2058
                     /locus_tag="PKH81_03520"
     CDS             1585..2058
                     /locus_tag="PKH81_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNQ97136.1"
                     /translation="MKERMTRLKTIRKLIKTYRIESQEALLGHLSREGFAVTQATLSR
                     DLKLLKVGKLSDGHNGYVYTLPGDEERQESEQTVVQDFLRGYISIDHSGNMVVIKTYS
                     GHSDVVAGAIDSMNMDEVLGSISGRDNCVFVCLREGVTGDDFLETLKLKIPELDE"
     gene            2116..>2820
                     /gene="pgi"
                     /locus_tag="PKH81_03525"
     CDS             2116..>2820
                     /gene="pgi"
                     /locus_tag="PKH81_03525"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758943.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ97137.1"
                     /translation="MINWNNLDTLSAYKKLLSLKGQVRLGEVLSAARVSSCQVPVAAG
                     LTYNYASKQVNDQILTVLQELADEQELIAKYKALLSGETINTGEGRKVLHHLARGQLV
                     GDVVFEGKNLRSFYREQQDKIATFVSRVHNGEITGTGGERFTTVVQIGIGGSDLGPRA
                     MYLSLNEWAVRSGVKKMDAKFISNVDPDDGAAVIGSCDLGRTLFILVSKSGTTQETLS
                     NELFVKDFLSKAGLEPS"
BASE COUNT          673 a          669 c          687 g          791 t
ORIGIN      
        1 tcattttgcg cgccccgaag atggcggctc cggaaagact gtcgttaccc tgggctagta
       61 cgctattttt tgtagggttc attaacaatc gctcccctgg ttttatccag gcggaacacg
      121 acttgtttca ttattttggg aacagtatag ggtacgtgac atatctcaat ttcaaccccg
      181 gggtatattg aaccgcgcac ctctacatat gcttgatcgt tggtgtcgat tctgggaagg
      241 aatgtgggta ttttagcggt aatctctgag cgtttttcta tcagactttg gatgtacgca
      301 cgtatcttat ctgacggctc atccttaatc atttcttcgg ccttcataag attaacggcg
      361 atgtgtttaa gctgctcatt ggctcgatca agttcttgct gtacgataaa atcggtaccg
      421 caacgtaaat acgtttttgc cccgcgcgga cttccaatat caagggcctg aactccgcca
      481 ataacgatgc actcgcaacc gacgagtttc ccggtgtctc ccatcttgat catacccatg
      541 gtatacactg tggactggat aatgcttccg gaaacgagta tatcgccgcg tacggcgacc
      601 cggcagtttt gaatgtattt cgcggtcaag ttaccaccga tgcgtagcgc cccgaccttt
      661 ttcccttcta tgcccgcctg gacactcatg tccttcttgc acacaacctc ggttgttacg
      721 tccaatacct cgctcgaaat aatcgagccg cctgagtata cgcgaaatcc gtcggcgatt
      781 tttccctgaa gtatgacatc tccgggaaag gcgatattac cggtattaaa atcgacccct
      841 tttttaagga gcagtacatc ctcaatagaa ataacgccct tgctgtcgat ggaaagccgt
      901 ccgctttttc cggcaaaaaa tcccttttcg tgacttatca cattttttcc ggcggtgtat
      961 accggcaggg tttgaacggg gtaggggact tcttccccgt aaatgttttt tccgggttcc
     1021 cctggaacct ccgggataaa cttggctatg ggatcttttg catgcacaat cgtaaaggcg
     1081 cttaactcat gccaatcgat cttttctgct tcgctgtcaa tctttggtgg tttttgcagt
     1141 aagtcctggc gaataagata gtgttccggg atacccgtaa ccggtggggt gctttgagca
     1201 atcgttacct cttttacggg tttgtggctg gagttggcgg caagaattga ctcctggatg
     1261 gcatcatgga gaattccggc tattattccg ttttcttcaa gacgttgaga aacaagccca
     1321 taggttaaaa aagagccacc agtagccgga ggataaaagg tcgcggttgc aacaagagcg
     1381 ccgccatgga cggttatctc ggcatatcca tcttttggat ctccttgccg aatgtagagt
     1441 atggtgtttt cttgattttc tgtgctttta tcgtccgcca cgcttagtcc cccgttacag
     1501 tatcggatac tgcttccctt gacattaccg ctatctttat acattatata tgtataaata
     1561 ttcaaacttg gatggcagta atcagtgaaa gaacgaatga cgcgactgaa aacaatccga
     1621 aaactcatta aaacataccg gattgaatcc caggaagcac tgctcggaca cctgtcgcgg
     1681 gaaggttttg cagttaccca ggcaaccctg tcgcgtgacc tgaaactgct taaagttgga
     1741 aaactgtcag acggtcacaa tggttacgta tatacgctcc ccggggatga ggagcgtcaa
     1801 gaatcagagc aaacagttgt tcaggatttc ttgcgaggtt atatctccat agatcattcg
     1861 ggaaacatgg ttgttatcaa gacctattca ggccattcag acgtggtcgc gggggctata
     1921 gactccatga atatggacga ggtccttgga agcatatccg gaagggataa ctgtgttttt
     1981 gtgtgcctca gagaaggtgt taccggggat gatttcctgg aaacgctcaa gcttaaaatt
     2041 cctgaactgg atgaataagc ggctctccgc ttattaccag ttttttttat caacatacaa
     2101 ctacaggaga ttctcatgat taactggaac aatcttgata cccttagcgc gtacaagaaa
     2161 cttttatcac ttaagggaca ggttcgcctt ggcgaggtat tgtccgccgc gcgggtttcc
     2221 tcctgccagg taccggtagc tgccggactt acgtataatt atgcaagtaa acaggttaat
     2281 gaccagatcc tgactgtgct tcaggagctt gccgacgagc aggaattgat tgccaagtac
     2341 aaggctctgt tatccgggga gacaatcaat accggagagg ggagaaaggt cctccatcat
     2401 ctggcacgcg gtcagcttgt cggtgatgtg gtatttgagg ggaaaaacct tcgctccttt
     2461 tatcgggaac aacaggacaa gatcgcgacc tttgtttcca gggttcacaa cggggaaatt
     2521 actggaaccg ggggagagcg ctttaccacg gtagtgcaaa ttggcatcgg tggttccgat
     2581 cttggtcccc gtgctatgta tctctcgctc aatgaatggg ctgttcgctc cggcgttaag
     2641 aagatggacg caaagtttat ttccaatgtc gatcctgacg atggtgcggc cgtcatcggt
     2701 tcgtgcgatc ttggtcgaac cctgtttatc ctggtatcta aaagcggcac cacccaggag
     2761 accctgtcca atgagctttt cgtaaaggat tttctttcaa aggctggtct tgaaccctcc
//
LOCUS       DAOQXQ010000163         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_38817_length_2806_cov_2.420938, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000163 DAOQXQ010000000
VERSION     DAOQXQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38817_length_2806_cov_2.420938"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKH81_03530"
     CDS             <1..406
                     /locus_tag="PKH81_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97138.1"
                     /translation="SFPFSGIPEGIKTLLSAIPESTPLLLSVYSPTRMGVQYWAREGE
                     GEPLEGKAVTYQDQSAVLFPDGTLYLAADGMSQSGKTLRLPRLAEGYIYTYFMISGSK
                     LVAAWEERRFFETGRAGLLEIPLPDGIYWKEE"
     gene            403..1134
                     /locus_tag="PKH81_03535"
     CDS             403..1134
                     /locus_tag="PKH81_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97139.1"
                     /translation="MKNLPATLVALFLVFLCIPVQADYSPWTFFGGVNFIYNSDGSDV
                     TEVPGMQEGGVPGGLDSAPSPITGLIGLEYRREIRERVFFCPSISLFFVEYLWANDQA
                     LPAEIENRTAFVPSLLLDASFVYTIERNRFLFSVGGGPGILLRWAFLDSGVSEDEQSY
                     STDMSAGDQVDHINSYMWSSLRWFYPMLHAGVRYRLETGWGGGLTLRTGIPLFNLWSS
                     PDVPFPHALMFIACITITPPLRSGQ"
     gene            1184..2560
                     /locus_tag="PKH81_03540"
     CDS             1184..2560
                     /locus_tag="PKH81_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97140.1"
                     /translation="MEQTNGFTDLLFQAITERQQLFDSFLLPKLQEEYRIAQSSAKTI
                     RTVLVKKGILRDDPYKYDSKISEIEIPSEEAFADSEKTTVIGIRLAHYEAMLDFINNY
                     YQFTCDYLNTDRINKLIQLNRVFSWESFSPTSTRVNTKGLADLINTLRSGADPLSISI
                     INDSLTQLSRCSISITKALKNLTDFHRERYKIAIRKLVLPGVIIDVQTLSGGTSQAIA
                     EIKRSFAVTMKGQPFYIELVEEVLKEEYSPDHAVLQQELLSRLSVSKIDNSKPAAEES
                     FKPVLLDGIRTLGAASPQLEELYNKFTENQRLVLSSEKSFFEKLARYVRKAFNLPEEA
                     QVITITTVDPATQTGKRETVDFTVFAEDLRRRARILTGFTVRTSPAYQKIEMMEEQQI
                     MELLTRQIAEMGNVLKTASGLDDYYKQWGSGSVHDHIRGVKVEISAIRNNLVKANQCR
                     ADYSHSGL"
BASE COUNT          673 a          765 c          671 g          697 t
ORIGIN      
        1 ctctttcccc ttctccggca tccccgaagg aatcaaaacc cttctctccg ccatccctga
       61 atctaccccg ctcttgctat ccgtgtactc tcccaccaga atgggagtgc agtattgggc
      121 acgtgaggga gaaggagagc cactggaggg aaaggccgtt acctatcaag atcaaagcgc
      181 ggttcttttt cccgacggta ccctgtatct ggcagcagat ggcatgagcc agtcaggcaa
      241 aacccttcgt cttccccgtc ttgccgaagg ctatatctac acctatttta tgatttccgg
      301 atcgaagctg gtcgctgcct gggaagaacg ccgctttttt gaaactggcc gcgcaggttt
      361 actggaaata cctcttcccg atgggatata ctggaaggag gaatgaaaaa tctacccgcc
      421 acgctggtgg cgctttttct tgtctttctc tgcatccccg tccaggcaga ctattcgccc
      481 tggaccttct ttggtggcgt taactttatc tataactccg atggatctga cgtaaccgag
      541 gtccccggca tgcaggaagg cggagttcct ggaggcctcg acagcgcgcc tagtccgatt
      601 accggcttaa tcggccttga atatcgccgg gaaatacggg agcgcgtctt cttttgccct
      661 tctatctcgc ttttttttgt ggaatatctt tgggcaaacg atcaagcgct tcctgcggag
      721 atcgagaacc gaaccgcctt tgttccctcg ctccttttag atgcttcctt tgtctataca
      781 atcgaacgga atcgtttttt attctcggtt ggtggcggcc ctggcatatt acttcgctgg
      841 gccttcctcg actccggggt aagcgaggac gaacaaagct atagcaccga catgagcgca
      901 ggagaccagg tagatcacat caattcctat atgtggagtt ccctgcgatg gttctatcct
      961 atgcttcatg cgggagtccg ataccgcctg gaaaccggct ggggcggcgg attaaccctg
     1021 cgaacaggta tccccctctt taatctgtgg tcctctcccg atgttccctt ccctcacgct
     1081 cttatgttta tcgcctgtat taccatcacc ccgcccctac gctcgggcca ataagggcgc
     1141 taacgcttgc cgttttaccg tttttatccg atactactgt agtatggaac aaaccaacgg
     1201 ctttaccgac ctgctttttc aggcgataac cgaaaggcaa cagctttttg attcgtttct
     1261 cctgcccaag cttcaggaag aataccgtat agcacaaagc tccgcaaaaa cgattcgcac
     1321 cgttctggta aaaaagggta ttctccggga tgacccctat aagtacgaca gtaaaatcag
     1381 cgaaattgag attccgtccg aggaggcatt tgccgattcg gagaaaacaa ccgttattgg
     1441 catcaggctc gcccattatg aggcaatgct cgactttatt aataactact atcaattcac
     1501 ctgcgattac ctgaataccg acagaatcaa caagttgatt caattaaacc gtgtgttctc
     1561 ctgggaatcc ttttcgccaa cctctaccag ggtgaatacc aagggacttg cggatttgat
     1621 taacaccctc agaagcgggg ctgatcccct gtccatcagt attatcaatg actcgctaac
     1681 ccagctgtcc cgctgttcta tttccatcac caaggcactt aaaaacctta ccgattttca
     1741 tcgggagcga tataagatcg cgatcaggaa actggtgctg cccggggtta tcattgacgt
     1801 gcaaaccctc tccgggggaa catcccaggc tattgccgag ataaagcggt ctttcgccgt
     1861 taccatgaag ggtcaaccat tttatattga actggtagaa gaagtcctta aagaagaata
     1921 ttcccccgac cacgcggttc tccagcaaga gcttctttcc cgtctgtcgg taagcaagat
     1981 cgacaactcc aaaccagccg cggaggagtc atttaagcct gtgctcctgg acggcatccg
     2041 caccctggga gcggcatccc cacagctcga agagctctac aataaattca ccgaaaacca
     2101 gcgattagta ctcagtagcg aaaaatcatt ctttgaaaaa ctggcccgat atgtccgtaa
     2161 ggcttttaat ttgccggaag aagcgcaggt aatcaccatt accactgttg atccggcgac
     2221 ccaaaccggc aaacgggaga ccgtagattt tacggtcttt gcagaagatc ttaggcgcag
     2281 ggcgcgcatt cttaccggtt ttaccgttcg tacctctcct gcctatcaaa agatagaaat
     2341 gatggaagaa caacaaatta tggagctgtt aacccgccaa attgcggaga tgggcaatgt
     2401 gctcaagacg gcgagcggtc ttgatgatta ctataaacaa tggggtagcg gcagcgttca
     2461 cgaccatatt cgcggtgtta aggtcgagat atccgctatc agaaataatc ttgttaaagc
     2521 aaaccaatgc cgcgctgact actcgcattc ggggctgtga gagaatccgc gcgtcgtgaa
     2581 aaacattcta tataacaatg cgttgcgacg cgcgaccgat tccgttttga agaaactgaa
     2641 taaagctcgg tttttgcctg ctcaggctgc ggcggttttg ccccaggcgg cgaaatgcgg
     2701 attggcgaaa tcggctttgc catagcgcag cgcagggccg tcgagcgtgc gtacgatgcc
     2761 gcccgccgca cgcagcacgg cgtcaccggc ggcgatgtcc cattcc
//
LOCUS       DAOQXQ010000164         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_38847_length_2805_cov_2.240000, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000164 DAOQXQ010000000
VERSION     DAOQXQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38847_length_2805_cov_2.240000"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PKH81_03545"
     CDS             complement(<1..989)
                     /locus_tag="PKH81_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNQ97141.1"
                     /translation="MAVYLFCKSCKSTYGLKLRECPKCGTPTAARDKVYRVSVMVNGK
                     RVTRTVPNSIDRAREIEAKLKVELVSDEYYDRREKIPTFAEAWESYSTLHKTGKSWKQ
                     DKSRYETTLKDRIGKKPLDQISPMDVHRLISDMKNATTKRKAAYSPKSIKNTHELMSR
                     IFNHAINVMSCKAENPCSKAKPKRSISEVTNLLDEEKLRSLLNTLDRHEDRPTANIVK
                     LLLFTGMRRGEAFSLTWQRVDLKSKWIYLDDPKSGEPEKIPLNELAYLVLKDQERFRP
                     EKTDLVFPNRKDGKRTDIKRPWAKIKKDAGLPAAFRLHDLRHQYASMLAASGEV"
     gene            complement(1209..2111)
                     /locus_tag="PKH81_03550"
     CDS             complement(1209..2111)
                     /locus_tag="PKH81_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97142.1"
                     /translation="MAVEYWGQKNMRDIDDSEIEEFLNSIPNIGKKTKSNYQSHLKGF
                     WKFARKMKAVSADEMPLFEPIKYDLGFRTVTTWQLQRQVIEKVKEMTFSFNPRIWLAI
                     DMLATYTELRPDDIRRIQEKNYHNGFVTIFYPTKSKRKNQPWITIKLIDEHREVWEWI
                     QKTFPAINHEMYFFRHHKGRARAKPGDVFSDKYLARCWQRAAKEVGLEGVSLYPGTRH
                     TTITETAQLLGRDAAKQASGHRTNKAFERYNQAINDGAYQVVAKIRPKMTGEVIRLNK
                     KRRHKGHHKGTTDSGGIQNDKSLD"
BASE COUNT          652 a          679 c          624 g          850 t
ORIGIN      
        1 acttctccgc ttgctgcaag catggatgca tactggtgcc tcagatcatg tagtcgaaat
       61 gctgccggga gtcctgcatc cttcttgatc tttgcccaag gtctcttgat gtccgtcctt
      121 ttcccgtcct ttctgttcgg aaatacgaga tcggtctttt caggcctgaa tctctcctgg
      181 tccttaagca caaggtaagc gagttcgttc aaggggattt tctcgggctc cccgctcttg
      241 ggatcatcga gatagatcca tttcgatttg aggtccacac gctgccatgt gaggctgaat
      301 gcttcgcctc gcctcattcc agtgaacaga agcagcttga ctatgtttgc cgtaggacgg
      361 tcttcgtggc ggtcaagggt gtttagaaga ctccgcagtt tctcttcatc aagaaggttt
      421 gtgacttcgc tgattgacct tttcggcttc gccttactgc atgggttctc tgccttgcag
      481 ctcataacat tgatcgcatg gttgaaaata cgggacatta attcgtgcgt gttcttgatc
      541 gacttgggag agtatgcggc ctttcttttc gtggttgcgt ttttcatgtc gcttatcagc
      601 ctgtgcacgt ccatgggaga gatttgatcg aggggtttct ttccgatgcg gtctttcaag
      661 gttgtttcgt atcggctttt gtcctgtttc catgatttgc cggtcttgtg aagcgtggaa
      721 tagctctccc atgcctctgc aaacgtgggg atcttctctc tccggtcata gtattcatcg
      781 ctcaccagtt caaccttgag cttcgcttcg atctcccgcg cacggtcaat gctgttcggc
      841 acggtcctgg tgacacgctt gccgttgacc ataacagaga cccggtaaac tttgtctctt
      901 gctgctgtcg gcgtgccgca tttcggacat tctcttagct tgagaccata agtgctctta
      961 cagcttttgc aaaaaagata aacggccata gctacctccc cgtcgtacac acggtgtaca
     1021 aatagtgtac aatcgcctag ctattcggtg tacaatgtag caacccttgg taaacataat
     1081 gaataaataa atcaatcaag tggtaatgtc aagcaaccca aagaaataca tagaaaaaga
     1141 catggaaacg ggctcataac ccaaaggtcg gaggttcaaa tcctcccccc gctaccaaaa
     1201 ttatccaatt aatccaaaga cttatcattc tggatacccc ctgaatcggt ggtgcctttg
     1261 tggtgcccct tgtggcgcct cttcttgtta agacgaatta cctcgccggt catctttgga
     1321 cggattttcg ctaccacctg gtatgcacca tcattgattg cttggttgta tcgctcaaag
     1381 gctttgttcg ttctgtgccc cgaggcttgc ttcgccgcat cccggccaag caattgagcc
     1441 gtctcagtga tcgtagtatg ccgggttccg ggatacagcg agaccccctc aagccccacc
     1501 tccttagcgg ctctttgcca acaccgcgca aggtatttgt cgctgaatac atcccccggt
     1561 ttagcccttg cacgcccctt atggtgccgg aagaaataca tctcgtggtt gatcgcgggg
     1621 aatgttttct gtatccactc ccatacctcc cggtgctcat caatgagctt tatcgtaatc
     1681 cacggctgat tctttctttt ggacttggtg gggtaaaaga tcgtcacaaa cccattgtgg
     1741 tagtttttct cctggattcg tctaatgtcg tcgggcctta gctcggtata ggtcgccagc
     1801 atatcgattg caagccagat tctaggattg aacgagaaag tcatctcctt gactttctcg
     1861 ataacctgcc gctgaagttg ccaagtcgtc acggtcctga agccgagatc atatttaatg
     1921 ggttcaaaaa gtggcatttc atcagccgaa accgctttca tttttcgcgc aaatttccag
     1981 aaccctttga gatgagattg ataattggat tttgttttct ttcctatgtt ggggatggag
     2041 tttaagaact cttctatttc agaatcatct atatccctca tattcttctg gccccaatac
     2101 tcaacggcca tgttgatggt gtgctcaaat cttctgtaag ttactagatt atcatctctt
     2161 tattctttca ggtataaggg ggccagagct tgtacggact tgggtctttt tgcgctataa
     2221 tccttgatgt caaacttttc ctcgcgctcg tccttttcat accggagatg attcaaccac
     2281 tttgatgcca tgtcatagtt tgtaaatttt cgagagtgac ctctaccaaa tttaacaaat
     2341 atccttctgg cacgtatttc aggatgatct gggcaacaca cggcattcct gaggttatcc
     2401 ttcattcgat taccgcagat cggacatctt tctctggtat aaataacccc ttgcatacaa
     2461 cccccctcgc ccaaaaactg tgataatcga ttataacctc gcttggatcc gtgggcaagg
     2521 ttttcatgag gtatttttac cttcatatca tatatattct accgaagtga aaaatattga
     2581 tgaaaacgct attcaattaa atcaataagt taaaagattg ttaatttttg gggggaataa
     2641 taaaattact cctggtaaac atgtggatca ggaatggtag atatcgctaa gaaaaagtaa
     2701 ttgaaaaccc ccgaaggggg tcctgctggt cgtcttttga cgaagtaaaa cctaagataa
     2761 aacaaggtga tacagagggg gtgaaacaga taaggcgtta tcggt
//
LOCUS       DAOQXQ010000165         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_38862_length_2804_cov_2.770462, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000165 DAOQXQ010000000
VERSION     DAOQXQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38862_length_2804_cov_2.770462"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKH81_03555"
     CDS             <1..458
                     /locus_tag="PKH81_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNQ97143.1"
                     /translation="HGIPHELALPPKGLNRPLQRQAVSDRVLAIMGAYQVEAIVLAGF
                     LTIMSGNLISQFAGRMINLHPALLPKFGGPGMWGHHVHEAVLASGDSESGCTVHLVDS
                     GCDTGLILLQKKVPVLSGDTPESLANRIHAVEHEAVLEGTILLARSLQT"
     gene            718..1713
                     /locus_tag="PKH81_03560"
     CDS             718..1713
                     /locus_tag="PKH81_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNQ97144.1"
                     /translation="MITIWKQENRKLVEGTREDGPIWVDARNVTREDIRVLETEYGVD
                     TEIIMDILDQDELSRIDREDDYTLIIVRLPIFISANEVSYFTIPLGILLFPDMILTIC
                     WADCEVLHDLTNNRVRSLTLNDLPAFVIRVLARADLIFLRYLKEINRRSNSIETELQK
                     SVRNLELIQLLNLEKSLVYFTTSLKSNQLLFEKLMKTRLINLDEEDREWLEDVATDNR
                     QAIEMADIYSNILSGMMDAFASVISNNLSIVMKRLTVISLVLMIPTMITSLFGMNIKL
                     PMGDWNNYNALAVVLGICFASSLVAMILLGDRKKKKQRLDPVERKKLRRRNRAVE"
     assembly_gap    1787..1796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1847..>2804
                     /locus_tag="PKH81_03565"
     CDS             1847..>2804
                     /locus_tag="PKH81_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ97145.1"
                     /translation="MAKRALISVFYKDGVLELVQYLIEKGWEILSTGGTKKHLADAGL
                     PVTDVSAVTGFPECLDGRVKTLHPKIHAGLLAIRANAEHMKTMSKLSVDTIDLVCVNL
                     YPFFEKVQAGLSFDETVEFIDIGGPTMLRSAAKNHADVLVLTDVADYEEVKTALTAAE
                     QDTSKIPLDLKRRLAGKVFNLTSAYDAAVSRFMLGEAGFPVSEHSNLWPTYYTVPLTL
                     AQSLRYGENGHQKAALYLNADSKGAFGGMKQLQGKELSYNNMRDLDIAWKAACAYGEY
                     LTKLPAGFPATDLLTHDSAPVICVALKHNTPCGAALGKTVLEA"
BASE COUNT          777 a          702 c          658 g          657 t
ORIGIN      
        1 gacatggcat accgcatgaa ctcgcccttc caccaaaagg acttaaccgc ccgctacagc
       61 gacaggcagt ctcggatcga gtcttggcga taatgggagc atatcaagtt gaagcgattg
      121 tccttgctgg cttcttgacg attatgagcg gaaacctcat cagtcaattt gccgggcgaa
      181 tgattaatct gcatccagcc ctcctgccaa agtttggagg gccaggcatg tggggccacc
      241 acgtgcacga ggcagtactt gcttccgggg atagcgaatc gggctgtacc gtacatctag
      301 ttgactctgg atgtgatacc gggctaattt tgctgcaaaa aaaagttccc gttctgagtg
      361 gggacactcc cgaaagcctt gcaaacagga tccatgcagt agaacatgag gctgtgctgg
      421 aaggaacgat tcttctagcc cggtcactac aaacctaaac cctaatttac ttgccacagt
      481 ccatccggta tggtattctc tgtaaaacac atcccttaca atgaggaggc agtatcccat
      541 ggatacggaa cctggtagtc gtagatacga atatcaacct gacacacata tgcctccccg
      601 tacaacccgc atcttctgac attcagacaa tacgagttcc ccaggtggag gccggacagg
      661 cggagcgaga tacgcaccgc ttgcgaaacg cacaaagctt gagtggagac caatcccatg
      721 attaccatct ggaagcagga aaaccgtaaa ctcgtagagg gaacccgaga agacggacca
      781 atttgggtag acgcgcgaaa cgttacccga gaagacattc gcgtccttga aaccgaatat
      841 ggcgttgata ccgaaattat catggacatc ctggaccagg acgagttgtc gcgtatagat
      901 cgagaagatg actataccct gattatcgtc cgcctgccca tttttatctc tgcaaatgag
      961 gtaagttact ttaccatccc ccttggtatt ttgctctttc ccgacatgat tcttaccatc
     1021 tgttgggccg attgcgaggt tcttcacgat cttaccaata atcgggtaag aagccttacc
     1081 cttaacgacc tgccagcctt tgttatcagg gtgcttgcgc gggcggactt gatatttcta
     1141 cgctatctaa aagaaataaa ccgtcgatca aacagcatcg aaaccgaatt acaaaaatcg
     1201 gtgcgaaacc ttgagttaat ccagctcctt aacctggaaa aatccctggt ctactttacc
     1261 acctcgctca agagtaacca gctactcttt gaaaagctta tgaaaacccg cttgatcaat
     1321 ctggacgaag aagacaggga atggctcgag gatgtggcaa ccgacaaccg acaggcgatc
     1381 gagatggccg acatctatag caatatactt tcgggtatga tggacgcctt tgcctcggtt
     1441 atctcgaata acctgagcat cgtaatgaag cgactaaccg ttatttccct ggtcctgatg
     1501 atccccacga tgataacaag cctttttggc atgaacatta aactgcctat gggtgattgg
     1561 aataactata atgcccttgc tgttgtactt ggtatttgtt ttgcctcctc cctggttgcc
     1621 atgattcttc ttggggaccg aaaaaagaaa aagcaacgac ttgatccagt tgaacgaaaa
     1681 aaattgagac ggcgaaaccg ggctgttgaa taatcgagag cacccataag aggccaatat
     1741 cctgattgaa ggagggagcg taatagaaca tatacgaaag attggtnnnn nnnnnnaccc
     1801 tgtttttcgt atattgtggg gtatctatac aaaaggggta aacggcatgg caaaacgagc
     1861 gcttatcagc gtcttttaca aagacggcgt acttgaactc gtacagtatc ttatagaaaa
     1921 gggctgggag attctttcta ccggcggaac aaaaaagcac ctggcagacg caggcttacc
     1981 ggtaaccgat gtaagcgccg taaccggctt tccagaatgc ctggacggta gagtaaaaac
     2041 cctccatccc aaaatccacg cggggctgtt agcaattcgc gcaaacgccg aacacatgaa
     2101 gacaatgagc aagctttccg tagacaccat cgatctggta tgcgttaacc tctatccctt
     2161 ttttgaaaaa gtacaagcag gactttcctt cgacgaaact gtcgagttta ttgatattgg
     2221 tggacccacc atgctccgct cggcagcaaa aaatcatgcc gatgttctgg tgctaaccga
     2281 tgtagcggat tacgaggaag taaaaaccgc ccttaccgct gccgagcagg atacgtccaa
     2341 gattcccctc gatcttaaaa gacgacttgc cggaaaggta tttaacctga caagtgcgta
     2401 cgacgcggca gtatcccggt ttatgcttgg cgaggcaggc ttcccggtgt cggagcactc
     2461 aaacctctgg cccacctact acaccgtccc ccttacactg gcacagtcgt tgcgttatgg
     2521 agaaaacggc catcaaaaag cagcgcttta tctgaatgca gattcaaaag gtgcttttgg
     2581 cgggatgaaa caactacagg gtaaagaact ctcgtataac aacatgagag acctggatat
     2641 tgcctggaag gccgcatgtg cctatggtga atatcttacc aaacttcctg caggttttcc
     2701 cgcaacagac ttactgacac acgatagcgc tcctgttatc tgtgtagccc ttaagcacaa
     2761 caccccctgt ggcgccgccc tgggaaaaac cgttctggaa gcat
//
LOCUS       DAOQXQ010000166         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_39093_length_2792_cov_3.095360, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000166 DAOQXQ010000000
VERSION     DAOQXQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39093_length_2792_cov_3.095360"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..877
                     /gene="metA"
                     /locus_tag="PKH81_03570"
     CDS             11..877
                     /gene="metA"
                     /locus_tag="PKH81_03570"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNQ97146.1"
                     /translation="MWYKQFYNPRAMKQDIRPLKIAILNLMPTKEVTETQLLRLLGNT
                     PLQIEITLLTMASHESTNTSKEYLEAFYRTFESVKDTKYDGLIITGAPVENMPFEDVD
                     YWKEMTRIMDWSETHVFSTLHICWGSQAALYHKYGIDKHKLDDKVFGIFEHRVLNPRH
                     KLLRGFDDTFRAPHSRHTTIYTEDINKIPELEILAVSEEAGAFIVASRDGRAVYVSGH
                     AEYDADTLSREYLRDINKGLKIEVPRNYFPGDNPEKNPPVTWRAHANLLFVNWLNYFV
                     YQETPYDIEDIH"
     gene            complement(921..1724)
                     /locus_tag="PKH81_03575"
     CDS             complement(921..1724)
                     /locus_tag="PKH81_03575"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HNQ97147.1"
                     /translation="MSRLIALFVSYSFVGWVCEVIYCSILERRFVNRGFLRGPLCPVY
                     GFGALAVVFLLEPFAHSIITLFIAATLITSAIEYATGWFLETAFRTRWWDYSSFRFNL
                     HGRVCLLNSLLFGVMGVLGLRVLHPFLLLLIGSLPSVVLDGGVLAVSIAVCVDLSFTL
                     RNLSGFESRLASLKDSLDRLGDLDIRENLEGLRERLRLDARDASLRLLRAFPLAVSRE
                     HGAELDVLRRLHSALARSRRVAHAGHVDRALRPALLLLALVSLAYVFFR"
     gene            1794..2558
                     /locus_tag="PKH81_03580"
     CDS             1794..2558
                     /locus_tag="PKH81_03580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ97148.1"
                     /translation="MKRSEPFSVLYIDDHLVALNKASGLLVAQDRWDPDVLRLDNLAT
                     ERICTDGQRLYAVHRIDKDTSGLVIYARTEEAHRTLSMAFQEREIHKSYHCLVHGRPA
                     WDETEVSIPLRIDGDERHRTVRDRKGKESVTLFRYIGPAGPYSWIEAQPVTGRTHQIR
                     VHLQLSGLSIACDPLYGDGEPLYLSKIKRSWRGDSFEERPLLDRLALHAWKMKLNHPI
                     TGEALELCAPYPKDLDTTRKQLAKIFKTDPLGPLSE"
     gene            complement(2524..>2792)
                     /locus_tag="PKH81_03585"
     CDS             complement(2524..>2792)
                     /locus_tag="PKH81_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97149.1"
                     /translation="SRLKKSFGAMPARIMSDPIRLPEFQTGRYACSEIGMLLVCGSYR
                     AADFLRDCASGSLVDLPRPDMRHLERDGKTGAIIIRLKDRADRS"
BASE COUNT          797 a          758 c          690 g          547 t
ORIGIN      
        1 gaaggcccgc atctggtaca agcagttcta caacccccgc gccatgaagc aggacattcg
       61 tccgttaaaa atagccattc tgaacctgat gcctacaaaa gaagtaaccg aaacgcagct
      121 tttacgcctg ttgggaaata ccccgcttca gatagaaata accctgctta ccatggcaag
      181 tcatgagtcg actaacacaa gcaaggaata ccttgaagcc ttttatcgca cctttgaaag
      241 tgtaaaagac acaaaatacg acggactgat tattaccggg gcaccggtag aaaacatgcc
      301 ctttgaggat gtggattact ggaaggaaat gacacgcatc atggactgga gcgaaaccca
      361 cgtctttagt accctgcata tctgctgggg atcgcaggcc gcgctctatc ataaatatgg
      421 aatagacaaa cacaaactgg acgacaaggt ttttggtatc tttgagcatc gagtgctcaa
      481 tccacgacac aaactcctgc gcggctttga cgacaccttc cgcgcgccgc acagccgcca
      541 cacaaccatt tacacggaag acatcaacaa aattccggaa cttgaaattc tggcagtatc
      601 ggaagaagca ggggccttta tcgtcgccag cagagatggc agagccgtct atgtgtccgg
      661 tcatgccgaa tacgatgcgg atactctttc gcgcgaatac ctgcgggata tcaacaaggg
      721 tcttaaaatc gaagttcccc gaaactactt tcccggagac aacccggaaa aaaatcctcc
      781 cgttacctgg cgagcccatg ctaacttgtt gttcgttaac tggctgaact actttgtgta
      841 ccaggaaacc ccgtacgaca tcgaggatat ccactaatta ctgctcgcag gattgttggt
      901 aatcctgcag ggcagtttgc ttaacggaaa aaaacataag caagagaaac aagagctaac
      961 agtaacaatg ccggccgcag ggcgcgatca acgtgacccg cgtgcgcgac acgtcgagag
     1021 cgggctaagg ctgaatgaag gcgcctgagc acatccagct ccgccccgtg ttcccggcta
     1081 accgcgagcg ggaaagcgcg caaaagccgc aggcttgcat ccctggcgtc aaggcgaagc
     1141 cgttcccgca agccttccag gttttcccgt atatcaagat caccaagtcg gtcaagggaa
     1201 tctttaagcg aggcaagacg cgattcaaag ccggaaagat tcctgagggt aaagctcagg
     1261 tcaacgcata ccgcgatcga tacggcaaga actcctccgt ccaaaacaac ggacggaaga
     1321 gagccaataa gaagcaataa aaagggatgc aacacacgaa ggccaaggac tcccatcacc
     1381 ccaaacaaca gagaattgag caggcacacc cgaccgtgca gattaaagcg aaacgatgaa
     1441 tagtcccacc atcgggtcct aaaggcagtt tccaaaaacc atccagttgc atattcaatc
     1501 gcgcttgtta tcaaggtcgc ggcaataaaa agggtgatga tagaatgagc gaatggctcc
     1561 aacaaaaaaa caaccgccag ggcgccaaag ccatataccg gacacagcgg gccacggaga
     1621 aagccgcggt ttacaaaccg gcgttcaaga atggagcaat agataacctc gcacacccat
     1681 ccaacaaaac tataagaaac aaacagggca attaaacggg acaaagacac cagacacctc
     1741 cttgtacagc tcaaggatat ctccattggc aattttttgg tatagtctac catatgaaac
     1801 gaagcgaacc tttttccgtg ctctatattg acgatcatct ggtcgccctg aacaaggcgt
     1861 ccggattact ggtagcccaa gaccgctggg atcccgatgt gctgcgcctg gacaaccttg
     1921 cgaccgaacg catctgcacc gatgggcagc ggctctatgc cgttcaccgt atcgacaagg
     1981 atacctcggg tctggttatt tacgcccgaa cagaagaagc ccaccggacc ttgagcatgg
     2041 cttttcagga gcgggaaatt cacaagagct atcactgtct ggttcatggc cggcctgcct
     2101 gggatgaaac agaagtatcc attcccctgc gcattgatgg agacgagcgc catcgcaccg
     2161 tacgcgaccg caaggggaaa gaatctgtaa ccctgtttag atatattggc ccggcaggtc
     2221 cgtatagctg gatagaggca caaccggtta ccggccgtac ccatcaaatc cgggtgcatc
     2281 tgcaactgtc ggggctttct atcgcctgtg atccactgta tggagatgga gagccgctct
     2341 atctttccaa aataaaacgc tcctggcgag gagactcgtt tgaggagcgc ccgctattgg
     2401 atcgccttgc cttgcatgcc tggaagatga agttgaatca tccgataacc ggcgaagccc
     2461 ttgagctatg cgcgccgtat cctaaagatc tggataccac ccgcaagcaa ctggcaaaga
     2521 tttttaagac cgatccgctc ggtcctttaa gcgaataata atggcgccgg ttttaccgtc
     2581 ccgctccaga tgccgcatgt ccggacgggg caggtccacc agagaacctg aggcgcagtc
     2641 ccgcaaaaaa tctgcggcgc gataagagcc gcacaccaag agcatgccaa tctcgctgca
     2701 cgcataccgc ccggtttgaa actccggaag gcgtatggga tccgacatga ttctggccgg
     2761 catggctcca aatgattttt taaggcgact ga
//
LOCUS       DAOQXQ010000167         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_39173_length_2788_cov_2.898280, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000167 DAOQXQ010000000
VERSION     DAOQXQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39173_length_2788_cov_2.898280"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKH81_03590"
     CDS             complement(<1..980)
                     /locus_tag="PKH81_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ97150.1"
                     /translation="MKDTTSNKESLLRPATLCVHGGYDPGDHRHSVARPLYQSSAYSY
                     DSASAAAALFSGETEGNIYSRLGTPTTDEAETRVALLEGGIAAVAFSSGMAAASGFFL
                     NFLSAGDVIASSSHLYGGSMGLFSDTLPRLGIETRFFDPLDSKSLEAIMDSRIKVIMA
                     ENLANPSLSVPDHEGIVKIARAWQVPYLVDNTLATPLSSRPFDYGADFILHSTTKYMS
                     GHGDAIGGMIVDSGRFTFTHDRYPLMHDSAPNGKSYVEAFGERAFSTRLRMKTLMNLG
                     GCMAPFNAWLLLRGLESLHVRYERHLSNALFLASELSRDSRIAWVNHPSL"
     gene            complement(991..1686)
                     /locus_tag="PKH81_03595"
     CDS             complement(991..1686)
                     /locus_tag="PKH81_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HNQ97151.1"
                     /translation="MNSKLPAWVGLGAFMLSLAAGCMNAFTLVSLIALPVTHSTGTTS
                     AFSIALAHGDFSQALYLLGLIIAFVLGAVSSGLLIRDYHLKLGRRYGIALMSEAVLVT
                     LAFVSYQDDPMVTHFLLAFAAGLQNAMATTYSGAVVRTTHVTGILTDLGIQAARAITG
                     QRVDRPRIFLFIWIFVGFVTGSFFAAFFFPILGALVLLLVILLSGGTGLAYYVYRILA
                     MRKQSLTVKPGSQ"
     gene            1815..2645
                     /locus_tag="PKH81_03600"
     CDS             1815..2645
                     /locus_tag="PKH81_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97152.1"
                     /translation="MDFIQDYPLSTQELLHFPLMFPDALKKYHVPGIPWASIDGLYDY
                     ADYWGIQNGTVRTCLSRMKGEGMIVPVELRGITHYRASALQLSIMDNTQFRKKYAKKG
                     FIIAIFSFSKSQEKERTKMRSLLSYAGFVRFAQNSYICGGINDDELKEAIAKEGFSDN
                     VFFFSVPRIEESETTKLVSAWGIPERKKFLDTFFATLREFINASEQNPRDIFCRIGRA
                     WVAYIIHVNASEPPLPESVIPEDYPYTDIYTYLQGLSTKHGKTMYRHWRESNTTRKGT
                     "
BASE COUNT          861 a          651 c          629 g          647 t
ORIGIN      
        1 tctaacgaag gatgatttac ccaagcgata cgggaatctc tggaaagctc ggatgcgagg
       61 aatagcgcat tggaaaggtg ccgctcatac cgtacatgaa gactctcaag accgcgaagg
      121 aggagccagg cattaaaggg cgccatgcat cctcccaggt tcatcaacgt cttcatcctg
      181 agcctggtac taaaggctcg ctctccaaag gcttcaacat aggatttgcc gttcggagcg
      241 ctatcgtgca tcaaggggta tcgatcatgg gtaaaggtaa agcggccaga atcaacaatc
      301 atcccgccaa tcgcatctcc gtgcccactc atgtattttg tagttgaatg aaggataaag
      361 tctgccccat agtcaaacgg acgagaactc aaaggagtgg caagggtatt gtccacaaga
      421 taagggacct gccaagcgcg tgcgatcttt actatgccct catgatctgg gaccgaaaga
      481 gatggatttg caagattttc ggccattatt actttaatgc ggctatccat aatggcttca
      541 agactcttgc tgtccaaggg atcaaaaaaa cgggtttcga tgcccaaccg aggcagcgta
      601 tcggaaaaaa gccccattga tccgccatat aaatggcttg aagaagcaat aacatctccc
      661 gcggataaaa aattcaagaa aaaaccggag gctgcggcca taccagaaga aaaggccacc
      721 gctgcaatcc ctccttctaa aagagcaacc cgtgtttccg cttcgtcagt ggtaggtgtt
      781 cccaatcggg agtagatatt accttcggtt tccccagaaa aaagtgcggc tgccgcggag
      841 gcagaatcgt aggaataggc agagctttga tacaggggac gggcaaccga gtggcggtga
      901 tcgcctggat catagcctcc atgcacgcaa agggttgcag gacgtaaaag cgattcttta
      961 ttcgatgtgg tatctttcat ggagaaaaga ctactgtgag ccaggcttga cggtcaagct
     1021 ttgttttctc atcgcaagaa tacgatacac gtaataagcc aagccggtcc ccccggaaag
     1081 aaggataacc agaagcaaca ccagggctcc aagtatcgga aagaaaaaag cggcaaaaaa
     1141 actcccagtt acaaagccta caaaaatcca gataaataaa aagatcctgg gtctatctac
     1201 tcgttgaccg gtaatagccc tggccgcttg tatgcccaaa tcggttaata tcccggttac
     1261 atgtgtagtg cgaactaccg caccggaata ggtagtagcc attgcatttt gaagtcccgc
     1321 tgcaaaggca agtaaaaaat gagttaccat ggggtcatct tgatacgata caaaggcgag
     1381 ggttaccaac accgcttccg acatcaaggc aataccataa cgcctgccca gttttaagtg
     1441 atagtctcta atgagtaaac cactcgaaac agcacccaaa acaaaggcaa taataaggcc
     1501 aagtaaatac agggcttgtg aaaaatcgcc atgagccaag gcaatggaaa aagcggaagt
     1561 agtaccagtg ctgtgagtaa ctggcagggc aatcaaacta accagggtaa aggcattcat
     1621 gcacccggca gccaaggaga gcataaaagc tccaagacca acccatgccg gcaattttga
     1681 gttcatatct ttttataggc gtcacaacat aaaatgtcaa ggccggtaaa ctttttcatt
     1741 cacttgacga atgtatcaag cagtgttaca gttgccaata tatgtatatc actatgcgta
     1801 acaaggtgta ccacatggat tttatacaag attacccact ttccacacag gaattacttc
     1861 attttcccct tatgtttccc gacgcactca agaaatacca cgtcccggga atcccctggg
     1921 caagcatcga cggcttatac gattatgctg actattgggg aattcaaaac ggaacagttc
     1981 gaacgtgtct ttcgcgaatg aagggagaag gaatgattgt tcctgttgaa ctacgcggta
     2041 ttacccacta ccgtgcttct gccttacagc tttccataat ggacaacaca caatttcgca
     2101 agaaatacgc gaaaaaaggt tttattatag cgattttttc cttttcaaaa agccaggaaa
     2161 aggagcgtac caaaatgcgg tcactgttgt catacgcagg atttgttcgc tttgcgcaaa
     2221 actcatatat ctgcggcgga atcaacgacg acgaattaaa agaggcaatt gcaaaagaag
     2281 gtttctccga caatgtattc ttttttagcg tgccaagaat cgaggaaagc gaaacaacca
     2341 aattggttag cgcttggggc attccggagc gaaaaaaatt ccttgatact ttttttgcta
     2401 cgcttcgtga gtttatcaat gcatccgaac aaaacccgcg agatatcttt tgccgtatcg
     2461 gcagggcatg ggttgcatat attattcatg taaacgctag cgagccaccc ctgccagaat
     2521 cggttatacc cgaagattat ccttacacag atatatatac ataccttcaa gggctcagca
     2581 caaaacacgg aaaaacaatg taccgccatt ggcgggaatc aaatacaacg cgtaaaggaa
     2641 cataatatga ccaaaatctt gctagtcact ttctgtactc ttcttaccag ttttctctgg
     2701 agcgtagatt ttatctacct tgattccatt aacggcgaag atctggaatc cagatccacc
     2761 atcatccagg atgaatcagg aaccggat
//
LOCUS       DAOQXQ010000168         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_39412_length_2777_cov_2.724100, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000168 DAOQXQ010000000
VERSION     DAOQXQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39412_length_2777_cov_2.724100"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..871)
                     /locus_tag="PKH81_03605"
     CDS             complement(230..871)
                     /locus_tag="PKH81_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97153.1"
                     /translation="MPSIPTTEHQAAQPLDAQTIATIPPSSVFFFLESNVMGPQTNLR
                     LDLVVTIDPQQYWELPWKEKPTVARQIGAINSYAKENGLSALLVAPGRIGTTSPELGV
                     PVRFAEIDGFCVVCERAWDQGGYRPELSFGSHFFQDLVESEIFYAGIPDYSSEVESGG
                     VYNSAFFNNEINLYASIVPDSSLPPSLISVWKVQSLTFISDIVRGTCLCARFT"
     gene            complement(1202..1726)
                     /locus_tag="PKH81_03610"
     CDS             complement(1202..1726)
                     /locus_tag="PKH81_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNQ97154.1"
                     /translation="MMGNFFRLPVPTYFGQSPIIVRSSSLLEDSFGHAFAGKYESVFC
                     VNTGTPEDRLASFENAIRCVYASCMDESALAYRRQRGLEESDEQMAILVQRVSGSLFG
                     HFFLPAAAGVSYSFNAWKWHESIECDKGMIRLVAGLGTRAVDRTDSDYPRLIALGRPD
                     LPPTYATDSTRYCQ"
     gene            complement(1851..2066)
                     /locus_tag="PKH81_03615"
     CDS             complement(1851..2066)
                     /locus_tag="PKH81_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97155.1"
                     /translation="MTGESLAGRKRPGESIIRGFLIFAAGVSVLTTIGIVITLGKESF
                     PCGDNVVWQVSSVADHRRRGRTNKNYS"
     gene            complement(2070..>2777)
                     /locus_tag="PKH81_03620"
     CDS             complement(2070..>2777)
                     /locus_tag="PKH81_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ97156.1"
                     /translation="RFGKGEIDIANASRGIKKSEAEKASASFGSPALEIRVGTDALAV
                     CVSKKNKFAKDMSMEEIALAFSTAVYWSDVRASWPKKEIKRYTPGTDSGTFDYFTEEV
                     LKKKKDLLLNAKNLQMSEDDHVLVQGIEASEYAIGFFGYAYYVENRKKLNAISVNSVT
                     PNQGTVDNATYPLARPLFLYTTAKTLNEKPQIAAFIAFYLSNVNRVIKRVGYFPAPQE
                     DLKKAKNAYLDAVKGRY"
BASE COUNT          670 a          662 c          716 g          729 t
ORIGIN      
        1 tgtatggttt tggcaaagat tcgactgtcg cctatgacgg ccacgcgggc ttcgttgtgg
       61 atgctttgtt gggataggga ttttataatt atttccggcc cgattcccgc aggatcacct
      121 gaggtgacgg cgataagggg tctattcatg attattccgc ctttggtttt cttatttcta
      181 tgctacctga aaagagattg acggcgcaag ctattcagcc tttttgcgct tacgtaaagc
      241 gtgcgcacag gcaggttccc cgcacgatat cggagataaa ggtgagcgat tgcactttcc
      301 ataccgaaat gagcgacgga ggaagacttg aatcaggcac gatggaggca tagagattga
      361 tctcgttgtt aaaaaaagct gaattataca cgccgccaga ttcgacttca cttgagtaat
      421 cggggatacc ggcataaaaa atttctgatt ctacaaggtc ctgaaaaaag tggctgccaa
      481 aggaaagttc cggccggtat cctccctgat cccaagctct ctcgcagact acgcaaaaac
      541 cgtctatttc cgcaaagcga acgggtacgc ccagttcggg agatgtagtg ccgatgcgtc
      601 ccggcgcgac gagaagggcc gaaagcccat tttctttcgc gtaggaatta atcgcgccga
      661 tctgtcgcgc taccgtcggc ttttccttcc agggaagctc ccaatattgc tgcgggtcga
      721 ttgttactac aagatcaagg cggaggttcg tttgcggccc catgacgtta gactccaaaa
      781 agaaaaaaac cgaggacggt ggaattgtgg caatggtttg cgcgtccaga ggctgagcgg
      841 cttgatgctc cgtggttggg attgaaggca tttgcaatgg acgacattgc agtaagttta
      901 tcagccgctc tccgtgagag ttccagttta cggtaaactc gatatccacc ggatttttat
      961 attcggtttg gagaaccagg agtatccgtg aaatatcctc gataaaaccg ctttctttgg
     1021 cgattccact gcaggtggca acgtataccg gccgaggcgt gcctttctct gcaaggtatc
     1081 gttgtgcatt ttcatcttct tcacagatac gttcgatgat aaaggggctt aaccaaggaa
     1141 gtacatcgtc gagtgacctt gtttcaagtt cccccgattc aaggttgagt aggtcaatgg
     1201 atcattggca atagcgcgtt gagtcggtgg cataggttgg aggtaagtca ggacgaccaa
     1261 gagcgataag gcgggggtag tcgctatccg tcctgtctac tgcacgagtg ccaagcccgg
     1321 cgacgaggcg gatcattcct ttatcgcatt caattgattc gtgccatttc catgcgttaa
     1381 acgaatagct aacaccagct gcggccggaa ggaaaaaatg accaaaaaga gatccggaga
     1441 cgcgctgtac cagaatcgcc atttgctcgt cagattcttc cagtcccctt tgcctgcgat
     1501 atgcgagcgc ggactcatcc atgcagcttg cgtagacaca ccttattgcg ttttcaaaag
     1561 aggcaagacg atcttcgggc gtgccggtat tcacgcagaa tactgattcg tattttcctg
     1621 cgaaggcatg gccaaagctg tcttccaaaa gactactgga tctgacgatg atggggcttt
     1681 gcccaaagta ggtaggtaca ggtaaccgaa aaaaatttcc catcatcaaa tccgcgtccc
     1741 aggcggtttg taagtccgac agggaatcaa agatataaaa ggcttcccgt cctgcagtag
     1801 caataatccg atgaacctct gcggtaaagt ttttaaaacc gacttgcgga tcaagagtaa
     1861 tttttgttag tcctgcctct tcggcgatga tcggctacgg atgaaacctg ccatacaacg
     1921 ttgtccccgc acgggaaaga ttccttgcca agggtaataa cgattcctat cgtcgttaag
     1981 accgaaactc ccgcggcaaa gattaaaaag ccgcgtataa tcgattcacc cggcctcttt
     2041 cggccggcaa ggctctctcc cgtcattgat cagtaccttc cttttacagc gtccagatag
     2101 gcattctttg cctttttcaa gtcttcctga ggagcgggga agtatccaac gcgcttgata
     2161 acgcggttta cgttactcag atagaaggca atgaaggcgg caatctgcgg tttttcgtta
     2221 agggtctttg ctgtggtgta caggaacagg ggcctagcaa gcgggtatgt cgcgttatca
     2281 acggttcctt gatttggggt taccgagtta acggatatcg cattcagttt tttccggttt
     2341 tccacatagt aggcgtagcc aaagaagccg atcgcgtact cggacgcttc gattccctga
     2401 acgagcacat gatcatcttc ggacatctgc aagtttttgg cgttcagtag aagatctttt
     2461 ttcttcttga gtacttcttc ggtaaagtaa tcaaaggttc ccgagtctgt gccgggggta
     2521 tagcgtttga tttccttttt tggccaggaa gcgcgtacat cagaccaata cacggcggta
     2581 gagaaggcaa gcgcaatctc ttccatgctc atgtcctttg caaacttgtt tttcttggat
     2641 acgcagacgg caagggcgtc ggtgccaacc ctgatttcta atgccgggga cccaaaggag
     2701 gctgatgctt tttccgcctc tgatttcttg atgccccgtg aagcgttggc aatgtctatt
     2761 tctcccttgc caaagcg
//
LOCUS       DAOQXQ010000169         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_39594_length_2768_cov_2.564689, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000169 DAOQXQ010000000
VERSION     DAOQXQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39594_length_2768_cov_2.564689"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /gene="rplE"
                     /locus_tag="PKH81_03625"
     CDS             complement(<1..267)
                     /gene="rplE"
                     /locus_tag="PKH81_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ97157.1"
                     /translation="MAAARLKIDYDTNIKKRMMDRFKYTSVMQVPRIEKIVLNVGMGD
                     GHSNQNALNAAIEELSRITGQHAVKTVAKKSIANFKIREGYDVGC"
     gene            complement(278..598)
                     /gene="rplX"
                     /locus_tag="PKH81_03630"
     CDS             complement(278..598)
                     /gene="rplX"
                     /locus_tag="PKH81_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ97158.1"
                     /translation="MASTKLKKNDNVVIVSGADRGKKGKILFVDTSKGRVVVEGINKK
                     KKFVRPSQENPKGGVISLEYPVEISKVMIFCDKCKKGVRVGMEIKDSGKNRVCKKCGK
                     SLDK"
     gene            complement(611..1003)
                     /gene="rplN"
                     /locus_tag="PKH81_03635"
     CDS             complement(611..1003)
                     /gene="rplN"
                     /locus_tag="PKH81_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ97159.1"
                     /translation="MIQVQTMLNVADNSGVKRVQCIKILGGSRRRYATVGDIIVVAVK
                     DSQPSYGLKDSTGKKVHTKAVLRAVVVRTSKPVRRKDGSYIRFDDNAVAIIDAKNEPK
                     GSRIFGPVARELRDRNFLKIVSLAPEVL"
     gene            complement(1013..1273)
                     /gene="rpsQ"
                     /locus_tag="PKH81_03640"
     CDS             complement(1013..1273)
                     /gene="rpsQ"
                     /locus_tag="PKH81_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNQ97160.1"
                     /translation="METAKKNKKQIVGKVVSNKMDKTIVVEIEGLMMHSLYKKSVRMT
                     KRIKSHDIKNECSVGDLVRVEETRPLSRDKRYRLVEIIEKVK"
     gene            complement(1300..1509)
                     /gene="rpmC"
                     /locus_tag="PKH81_03645"
     CDS             complement(1300..1509)
                     /gene="rpmC"
                     /locus_tag="PKH81_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ97161.1"
                     /translation="MKGKLEELNIEELDRSMREAKEEIRKQRFKSVTSKVDNPKRVAA
                     LKKHIARILTLKREYTLGLRAPKGK"
     gene            complement(1529..1939)
                     /gene="rplP"
                     /locus_tag="PKH81_03650"
     CDS             complement(1529..1939)
                     /gene="rplP"
                     /locus_tag="PKH81_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ97162.1"
                     /translation="MLMPKRTKFRKLQRGRMKGKAFRGSTISFGEFALKSMECGKISS
                     KQIEAARVAISRKVKRGGKLWIRIFPDYPVTKKPAETRMGKGKGNPEGWVAIIKPGRI
                     LFEIAGVSEELAKDALRLAAFKLPVKTKITGIEK"
     gene            complement(1954..2634)
                     /gene="rpsC"
                     /locus_tag="PKH81_03655"
     CDS             complement(1954..2634)
                     /gene="rpsC"
                     /locus_tag="PKH81_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNQ97163.1"
                     /translation="MGQKVNPTGLRVGVNRTWDSVWYEEKKQYSRNLHEDLAIQRHVQ
                     QKHKNAGIYKVAIERFPDRVNVNIHASRPGLLIGKKGSDIEVLKAELQKISSKNVYIN
                     INEVKKPEKNAKIIAEQVASQIVGRFPYRRAAKQAIAGAIRGGALGIKVAISGRLNGA
                     EISRAESYKEGRIPLHTLRADIDYGTAEALTTFGLIGIKVWIYNGDILSKKEESDEDK
                     YTVKRKTK"
     gene            complement(2653..>2768)
                     /locus_tag="PKH81_03660"
     CDS             complement(2653..>2768)
                     /locus_tag="PKH81_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HNQ97164.1"
                     /translation="TVDEGPTMKRFRARARGRASRLRKRTSSIVVILSNEN"
BASE COUNT          502 a          854 c          608 g          804 t
ORIGIN      
        1 gcatccgacg tcgtatcctt cacgaatctt gaaattcgcg atggatttct tggccacggt
       61 ctttaccgcg tgctgtccgg taatcctgga aagctcttcg atggcggcgt taagggcgtt
      121 ctggttggaa tggccgtcac ccatgccgac attgagaaca atcttctcaa tcctcggaac
      181 ctgcatcacc gatgtatact tgaagcggtc catcatccgc tttttgatat tcgtgtcgta
      241 atcgatttta agccgtgccg cagccatctc gttcacccta cttgtccagg ctcttgccgc
      301 attttttgca aacgcgattt ttgccggaat ccttgatttc catcccaacc cttacgccct
      361 ttttgcattt gtcgcagaag atcatcacct tcgatatttc gacgggatat tcaaggctta
      421 tcacccctcc ctttggattt tcctgcgaag ggcggacgaa ctttttcttc ttgttgatcc
      481 cttcaacgac aaccctcccc ttggaggtgt cgacaaagag tatcttcccc ttttttcccc
      541 tatcggcgcc cgatacgatg acgacgttgt cgttcttctt taacttcgtg cttgccattg
      601 caattgcctc ttataacact tccggggcca gagaaacgat cttcaggaaa ttgcggtccc
      661 tgagctcccg cgccaccggc ccgaaaattc gcgatccctt aggctcgttt ttggcgtcta
      721 ttatcgccac ggcattgtcg tcaaagcgta tgtacgatcc gtccttcctg cggacaggct
      781 tgctcgtccg aacgaccacc gcccttagca ccgccttggt gtgcaccttt ttcccggtcg
      841 aatccttcaa tccatatgag ggctgcgaat ccttgacggc caccacgata atatcgccga
      901 ccgtcgcgta acgcctccgc gatccgccga gaatcttgat gcactgcact ctcttaacgc
      961 cgctgttgtc ggcgacattc aacatcgtct gcacctggat catgacaaac ccctacttca
     1021 ccttctctat aatctctacc agacgatacc gtttgtccct ggaaagcggc cttgtctcct
     1081 caacccttac aaggtcgccc accgagcatt cgttttttat atcgtggctc tttatcctct
     1141 tcgtcatcct gactgacttc ttatacagcg agtgcatcat gagcccctct atctcgacaa
     1201 cgatggtctt gtccatcttg ttgctcacca ctttgccgac gatctgcttc ttgttttttt
     1261 tcgcggtctc catgaaacgc taacgctcct gatgaattgt tatttccctt tcggcgccct
     1321 taagccaagc gtgtattccc gtttgagcgt caatatccgc gcgatgtgtt tcttgagggc
     1381 ggccacccgc tttggattgt ccaccttgct cgtaacgctc ttgaagcgct gcttgcgtat
     1441 ctcttctttc gcctctcgca tgcttctgtc gagctcttct atgttgagtt cctcgagttt
     1501 tcccttcatg tgcctaaccc tgtgcctctt acttttcgat accggtgatc tttgtcttga
     1561 ccggaagctt gaatgccgcc agcctcaatg cgtccttcgc cagttcctcg ctgacgccgg
     1621 ctatctcgaa gagaatacgg cccggcttga tgatcgcgac ccatccctcg ggatttccct
     1681 tccctttacc catacgggtt tcggcgggct tttttgtgac cgggtaatcc ggaaaaatcc
     1741 gtatccagag ctttccgcct cgtttcacct ttcgcgaaat ggcgacacgc gcggcctcta
     1801 tctgcttgct ggagatcttg ccgcattcca tggatttcag ggcgaactct ccgaatgaaa
     1861 tcgtcgatcc gcgaaaggcc ttgcccttca tcctgccccg ctgcagcttc ctgaatttag
     1921 tcctctttgg catcaacatg gcaaaaccct cgcttacttc gtcttacgtt taacggtata
     1981 cttgtcctcg tccgattcct ctttcttcga gagtatatcg ccgttgtaaa tccaaacctt
     2041 tattccgatc agtccgaaag tggtaagggc ctccgccgtt ccgtaatcga tgtccgcccg
     2101 gagggtgtgc agcggaatcc tcccctcttt ataggattcg gcccgggaaa tttcggcgcc
     2161 gttcaaccgt cccgaaatcg ccactttaat gcccagcgcc ccaccccgaa tcgcccctgc
     2221 tatcgcctgc ttggcggcgc gcctgtaggg aaagcgcccc actatctgcg aagcgacctg
     2281 ctccgcgatt atcttcgcgt tcttctcggg ctttttgacc tcgttgatat tgatatatac
     2341 atttttggag gatatcttct gaagttccgc cttcagcacc tctatatccg agcccttttt
     2401 cccgataagc agcccggggc gcgaggcatg gatgttgacg tttaccctgt cggggaaacg
     2461 ttcgatagcg accttgtata ttccggcatt cttatgcttc tgctgaacgt gcctctgtat
     2521 cgcgagatcc tcgtgcagat tcctggaata ctgctttttt tcctcatacc aaaccgaatc
     2581 ccatgtgcgg ttcactccga ccctgagacc tgtaggattg actttctgac ccatagccct
     2641 cttccgtgcc tcttagtttt cgttggaaag gatgacgaca atgctactgg tccgctttcg
     2701 cagccgcgag gcccggccgc gggcacgggc gcgaaaacgc ttcatcgtcg gcccctcatc
     2761 cacggtga
//
LOCUS       DAOQXQ010000170         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_39749_length_2761_cov_2.853289, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000170 DAOQXQ010000000
VERSION     DAOQXQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39749_length_2761_cov_2.853289"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PKH81_03665"
     CDS             <1..1247
                     /locus_tag="PKH81_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ97165.1"
                     /translation="FRVVESLNYANEVMGVIIFECNTLPYSAFGILREQISLTLHNIK
                     TFTRIAELNKSLSEEVSHLSSLRTIDRAITETRTRALLLDVLMNEILDQQKTDAIVIS
                     LVHQEGSEPEIAAWRGFRRLQPTGSRPVPAQRLLVRNLHQAGALGAEEPDIRNYFPEE
                     GFSFYCRVPLCAHGKTIGLVELFNRSPFDSTPGWEAFLDTLAGQAAIALDNARLMEDL
                     TQANVSLRQAYNTTIEGWSRALDLRDHETEGHCMRVAQMSVKLGKALGLPNAEIENLY
                     RGALLHDIGKVGIPDAILLKPGPLSDDERSIMSLHPTYAYDLLSPIEYLLPSLAVPWC
                     HHEKWDGTGYPQGLTGEDIPLSARIFAVIDVWDALSSERPYRGPWERGQIIAHIKEQS
                     GSHFDPSVVDAFLALVEKNQEI"
     gene            complement(1244..2413)
                     /locus_tag="PKH81_03670"
     CDS             complement(1244..2413)
                     /locus_tag="PKH81_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97166.1"
                     /translation="MAIKRVFLIAVSLSIALLSLVAQEDLPPGVAIAEDNYFEIEEDG
                     NARFMQVLSWKAQDHALHYEVEIQDSAGVEVYKATSTENTQTVSLLAGEYRWRLTVYN
                     LLGKKEASGPWQDLKVVKVESPRITGFSPETLYIEDGNFIVTLEGRNLTPDSRIYLAG
                     TISGIAWHDVQVLENDKGRRVVFSLDAEKLDGGVFTITVVHPSGLRAQSKKSLKIRYQ
                     TSQQFFASLSWAPVVPLLDEFYTDTWSDGLYPAGAEGAFTWLFVRRKASFMGAQMRAG
                     GILMDGGDDRALLETSQYRLSLSFAYKRIVTDRICVVVHAGAGLAFTDMTFLYDDVEG
                     TSLATMDALVCAGVSGQYLFTRHLFGECSLEAVNVFYDGANALSFIPALGLGYLF"
     gene            complement(2403..>2761)
                     /locus_tag="PKH81_03675"
     CDS             complement(2403..>2761)
                     /locus_tag="PKH81_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97167.1"
                     /translation="LVTPAKDAVFGPAELRGMKSLEFTWLPVSGATEYQFRLYQGLSK
                     KPLLERSELTGTSVVLDDLTVLDRGNLFWEVRALRKNSGGKTERDGLVARQRFTIDLP
                     KLTAPDAAGRKEYYGN"
BASE COUNT          703 a          768 c          667 g          623 t
ORIGIN      
        1 gctttcgggt tgttgaatcc ttaaattatg ccaacgaggt tatgggtgtt attatctttg
       61 aatgtaacac ccttccctac tcggcctttg gtattctgcg ggagcaaatc agcttgaccc
      121 tgcacaatat aaaaacattt acccgaatcg cagagcttaa caaaagcctc tccgaagaag
      181 tttcccatct ttcctcttta cgaacgatag accgcgccat taccgaaaca cggacacggg
      241 ccctgcttct tgatgtactg atgaacgaga ttcttgatca acaaaaaacg gatgccattg
      301 ttatttctct tgtgcatcag gaaggatctg aacccgagat agcggcttgg cggggcttca
      361 ggcgccttca accgacggga agccgtcccg ttcccgcaca gcggctcctg gtgcggaacc
      421 tccatcaagc cggagccctt ggcgcagaag agccggatat ccgcaactac ttccccgaag
      481 aaggctttag tttttattgc cgcgtacccc tttgcgcaca tggaaaaacg attggactgg
      541 ttgaactttt taaccgctcc ccctttgata gcaccccagg ctgggaagcc tttctggaca
      601 cacttgcagg acaagcggcc atcgcccttg ataacgcacg gcttatggaa gatcttacac
      661 aggcaaacgt gagcctgcgc caagcctata acaccaccat agaagggtgg tcgcgcgccc
      721 ttgaccttcg ggaccatgaa actgagggac actgcatgcg cgtcgctcag atgagcgtta
      781 aacttggaaa ggcacttggc ctgcccaacg cagaaataga aaacctgtac cgcggagcct
      841 tgcttcacga tattggcaag gtgggcatcc ccgacgccat actccttaaa cccggtccct
      901 taagcgacga tgagcgatcg ataatgagtt tacatcccac ctacgcctat gatcttctgt
      961 ccccgataga atatctgctg ccgtcgcttg cggttccctg gtgccaccac gaaaaatggg
     1021 acggtaccgg ctatccgcag gggcttaccg gagaagacat acctctttct gcccgcatat
     1081 tcgccgttat tgatgtgtgg gatgccctgt caagcgaacg tccctaccgt gggccctggg
     1141 agcgcggtca aattatcgcg cacatcaagg agcagtctgg aagccatttt gacccgtcgg
     1201 tagtggatgc ttttttggca ttggtagaga aaaatcagga aatctaaaaa agatagccaa
     1261 ggccaagcgc aggaataaaa gaaagagcat tggccccatc gtagaatacg tttaccgctt
     1321 ccaaagaaca ctccccaaac agatggcggg taaacaggta ttggccactg acgcctgcac
     1381 ataccagcgc atccatcgtc gcaaggcttg tgccctctac gtcatcatac aaaaaggtca
     1441 tatcggtaaa ggcaagaccg gctccggcgt gtactactac acatatccga tcggtaacga
     1501 ttcgtttata ggcaaacgag agggacagtc gatactggct cgtttccaac aaggcccggt
     1561 cgtctccacc atccatcaaa ataccgcctg cgcgcatctg tgcgcccata aacgaagcct
     1621 tgcgcctgac aaagagccag gtaaaggcac cttctgcgcc tgcgggatac aagccatcgc
     1681 tccacgtgtc ggtataaaac tcgtccaaca agggaacaac cggggcccag gacagcgagg
     1741 caaagaactg ctgacttgtt tgatagcgaa tttttaacga cttcttgctc tgtgcacgta
     1801 agccgcttgg atggaccacc gttatagtaa aaacgccacc atcaagtttt tcggcatcta
     1861 acgaaaaaac aacgcggcga cccttgtcgt tttccaacac ctgtacatca tgccaggcga
     1921 ttcccgagat agtccctgcc aaataaatgc gagagtcggg ggtaaggttc cggccttcca
     1981 gggtaacgat aaagttcccg tcttcgatgt ataaggtctc cggagaaaat ccggttatcc
     2041 ggggggattc aaccttgacg acttttaaat cttgccaggg gccactcgct tctttttttc
     2101 ccagaaggtt ataaaccgta aggcgccaac gatattcgcc ggcaagaaga ctcaccgtct
     2161 gcgtattctc tgtactcgta gccttgtaca cctctacgcc ggcgctatcc tgtatttcaa
     2221 cttcgtagtg aagcgcgtga tcctgggcct tccaggaaag aacctgcata aagcgtgcgt
     2281 ttccgtcttc ttcaatttcg aagtaattat cctcggcaat cgcaactccg ggaggcaagt
     2341 cctcttgggc aacaagagaa agaagagcaa tgctcaggct aaccgcaata aggaatactc
     2401 gcttaattgc catagtattc cttccttccc gctgcgtcgg gggccgtcaa ttttggtaag
     2461 tcaatcgtaa agcgttggcg tgcaacaagg ccatcgcgtt cagtcttccc gccactgttc
     2521 ttgcgaaggg cgcgcacctc ccaaaaaagg ttcccacgat ccaatacagt aaggtcatcc
     2581 agaacaacag aggtccccgt aagctcagag cgctccagca agggcttttt ggacaagccc
     2641 tgatacaatc tgaattgata ctctgtggcg ccgcttaccg gaagccaggt gaactcaaga
     2701 gacttcatcc cccgtagctc cgcaggtcca aagaccgcat ccttggcagg ggtaacaagg
     2761 g
//
LOCUS       DAOQXQ010000171         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_40101_length_2745_cov_3.142379, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000171 DAOQXQ010000000
VERSION     DAOQXQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40101_length_2745_cov_3.142379"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKH81_03680"
     CDS             complement(<1..462)
                     /locus_tag="PKH81_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ97168.1"
                     /translation="MQSIPFNIIFAIVLAGGWLSGKIFARLRLPAILGMVVFGIVCSL
                     TLQKSVSPLLWDITPYLKSFALMVILLRAGLGISRATLKKVGRTAFLMAWVPCICEAA
                     ALTALLYTVFSFSWPVAALTASMLSAVSPAVVIPSMLDLKARGRGSRNEVPT"
     gene            complement(482..2392)
                     /locus_tag="PKH81_03685"
     CDS             complement(482..2392)
                     /locus_tag="PKH81_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97169.1"
                     /translation="MLLKRSLTLIIVVLSLFALRTFFAVISNLDRYRQNRDFQITLNE
                     AKWSLETTKIILEEYLINSSPRSLYQLGAKKESFKNDSAFRKMDGWIDRYRLVAPMLS
                     KRRELLYIVDILVAQGGQAQQGDQLYSETAAQAFLLAHEMSSYMIDLSVESTDQISND
                     TRMFIGALTITLAFLIPFILFVIIWIRKRVFSRIILLDTASCRIADGDYSKEIDISGN
                     DELSRLAHSFSVMQRSVREHVVALAGETDRLSAILHSIGDAVIAVDRSGYIILMNPVA
                     ERLTGWRFGEAQGHPLAEVFNIIKEGSRDRQNSPVSRVIETGETVALANHTILVSRSG
                     EEYQITDAASPILTRTKEISGVVLVFRDVTEYNRMQSIMIQSEKMLSVGGLAAGMAHE
                     INNPLSGMLQTASVVETRLSEDIPANRDAAREAGISMDALRMYMEMRGIFRMLALMKE
                     SGSRIGRIVNNMLGFARKGPAQVSSHNLCGLVDRTLELAGTDYDMKKEYDFRKILIVK
                     QYEDDLPLIPCDAGQIQQVLLNLLRNGAQAMQETNTVSPRFLVKVYLDTSREYVVIEI
                     GDNGPGMSEEVKKRIFEPFFTTKPVGIGTGLGLSVSYFIISENHRGHMDVVSSPGEGA
                     TFIIKLPLPNSL"
     gene            complement(2382..>2745)
                     /locus_tag="PKH81_03690"
     CDS             complement(2382..>2745)
                     /locus_tag="PKH81_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97170.1"
                     /translation="LVTRLTTSIEREDDLVRIIESLERATSFLNTRSDVALDIVSESL
                     SLSRESLAKEWIEYSFFIELPQSLLLAMEQESSWYRSRRSVDFESPQYLEMIDDKPLA
                     RRYPRRVSIAGSGGVHAP"
BASE COUNT          812 a          659 c          580 g          694 t
ORIGIN      
        1 cgttggcacc tcgtttcttg atcctcgtcc ccgcgcttta agatcgagca tagagggaat
       61 taccaccgca ggagataccg cagaaagcat actggcagta agggcagcaa ccggccagga
      121 aaaggagaag acagtataca acagagccgt taatgcggca gcttcgcaga tacacggcac
      181 ccatgccatt aaaaaagcgg ttctacctac ttttttaagg gttgctctac taatgcccaa
      241 tccggccctg agtaaaataa ccatcagggc aaaagatttt aagtaaggag ttatatccca
      301 aagaagtgga ctcaccgatt tttgtaatgt aagggaacag acaataccaa aaacgaccat
      361 ccccaaaatc gccggaagcc tgagacgggc aaaaatttta cccgacaacc agcctcctgc
      421 aagtacgata gcaaaaataa tattaaaagg aatagattgc atgattgaca agtattgtca
      481 attatagtga attgggcaag ggtaatttta taataaacgt agcaccctct cccggacttg
      541 acacaacatc catgtgccca cgatgatttt cgctaataat aaaatacgag acactcaagc
      601 ccaagccagt gccaatgcca accggctttg tagtaaagaa aggttcaaag atccgctttt
      661 ttacctcctc gctcatgcct ggcccattat caccgatctc gattactaca tattctctgc
      721 tagtatccaa gtacacctta accaggaaac gaggagagac tgtattagtc tcttgcatgg
      781 cttgtgcccc attgcgtaat agattaagaa gcacctgttg tatctggcct gcatcgcacg
      841 ggataagagg aaggtcatcc tcgtactgtt ttacgatcaa tatcttgcgg aaatcatatt
      901 cttttttcat atcgtaatcg gttccggcaa gttcaagtgt ccgatccacc aggccgcata
      961 gattatgcga agaaacttgc gcaggcccct ttcgggcaaa gccaagcatg ttatttacta
     1021 ttcggcctat tctactgcca gactccttca taagagccaa cattctgaat attccgcgca
     1081 tttccatgta catcctcagc gcatccattg agattcctgc ttcccttgca gcatccctgt
     1141 tagcaggaat atcctcggat aaacgggttt ccaccaccga ggcggtttga agcatgccgg
     1201 acaggggatt atttatttcg tgggccatac ccgcggcaag acctccaaca gaaagcattt
     1261 tttccgattg aatcatgatg ctttgcatgc ggttatattc agtaacatcg cgaaaaacca
     1321 atacaacacc ggatatctct ttagtgcgcg ttaatatcgg agaagccgca tcggttattt
     1381 gatactcctc cccgcttcgg gaaacaagta tggtatgatt ggcaagggca acagtctctc
     1441 cggtttctat aactcgcgac accggactat tttgtctgtc ccgtgaacct tccttaataa
     1501 tattaaaaac ttcggcaagg ggatggcctt gcgcttcgcc aaatctccat ccggtcaatc
     1561 gctctgcaac aggattcatc aagatgatat agcctgagcg atcaacagcg attaccgcat
     1621 ctccaataga atgaaggatc gcggaaagac gatcagtttc tccggcaagg gcaacaacat
     1681 gctcgcggac agaccgctgc attacgctaa aagaatgcgc aaggcgcgaa agttcgtcat
     1741 tgcccgaaat atcgatctct tttgaatagt ctccgtcggc gatgcggcaa gaggcagtat
     1801 caagtagaat aatacgggaa aagacccgct tacgaatcca gataataaca aataaaataa
     1861 aggggataag aaaggcaagc gttatcgtta aggccccaat aaacatgcgg gtatcattac
     1921 tgatttgatc ggtggattct accgaaagat ctatcatgta cgaagacatt tcatgggcga
     1981 gtaaaaaagc ctgggcggct gtttcgctat acaattgatc tccttgctgg gcttgcccac
     2041 cctgggctac aaggatatcc acaatatata aaagttccct tcgctttgaa agcatggggg
     2101 caacgagtct gtaccgatcg atccagccat ccatcttcct gaaagcactg tcgtttttaa
     2161 aagattcctt cttcgcaccc aattgataca aggatcgggg gcttgagtta ataagatatt
     2221 cttccagtat tatctttgtt gtctcaagcg accattttgc ctcattcaag gttatctgaa
     2281 aatctctgtt ttgacggtat cgatccagat tggaaataac ggcaaaaaag gtcctaagcg
     2341 caaacaacga caagactact ataataagcg taagggatcg cttaaggagc atgtacacct
     2401 ccgcttcctg cgatagacac ccggcgagga taccttctgg caagcggttt gtcgtcaatc
     2461 atttccaaat actgtggaga ttcaaaatca acactccttc ttgaacgata ccacgaagat
     2521 tcctgttcca tggcaagaag caagctttgg ggtaattcaa taaagaaaga atattctatc
     2581 cattctttgg caagcgactc gcgcgaaaga gataatgatt ccgatacaat atcaagggct
     2641 acatcagatc ttgtatttaa aaagcttgtc gcacgctcaa ggctttctat aatgcgcacc
     2701 aaatcatctt cccgctcaat cgatgtagta agcctggtaa ctaaa
//
LOCUS       DAOQXQ010000172         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_40405_length_2732_cov_2.828913, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000172 DAOQXQ010000000
VERSION     DAOQXQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40405_length_2732_cov_2.828913"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKH81_03695"
     CDS             <1..211
                     /locus_tag="PKH81_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97171.1"
                     /translation="QTKWNHRLSVLPATSRVVIWRPVCRLRDGRTLVLENRRTLSGRL
                     PVIRCRKGACGQRRADVAESVTSRT"
     gene            complement(301..1212)
                     /locus_tag="PKH81_03700"
     CDS             complement(301..1212)
                     /locus_tag="PKH81_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ97172.1"
                     /translation="MAIIEAKGLSKTYQNAVKEAGLAGALKHFIKPVFTEIKAVSDVH
                     LSINPGETVAYVGPNGAGKSTTIKMLTGILKPSGGQIQINGMDPYRERLENTKHMGVV
                     FGQRTQLWWDIPVSESFTLLRDIYAIPPKRYKENLAWLSEMLGLTPFMGSSARKLSLG
                     QRMRADLALAFLHDPSVVFLDEPTIGLDVAVRERIREFLKKINRERGTTILLTSHDLD
                     DIRDVCDRLVVIDRGTIIYDGKLQALLDQCVRCRTMHLRLTSDGEEFAKKIGKHANLD
                     VIAKGGLLGAPARCTCPALIGAAYSKR"
     gene            1307..2638
                     /locus_tag="PKH81_03705"
     CDS             1307..2638
                     /locus_tag="PKH81_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97173.1"
                     /translation="MKTHAFVWYAVTAASMCLWLSCSSSDSGDSLDLSPSLSKGPPPL
                     CGIVLWQTNPMITLYKDSISLEFSYFYYSDVASDDPSGGYRYDWTKVDTFLTQAKGRG
                     HHGIVRFRDTDPELGLVDKSLPDSLWVHTRQANYDEGIAGAAKKRVVFPDWSNPDLPA
                     FISSFYKKMAERYPDQSTGLGYVEVGFGFWAEYHIDFDNLSNFSHADADTMAEALGRI
                     FPSRADQVSILTEVSRSFVNVPWGISVDAGDTDFGPYAADLPVNAPAFGIFDDSLLHG
                     EWNDTNWENWKFFEDRITAGMNGGEFSYYTDFDQAHALDSDGPHGLSLAEVARRCNLS
                     FVIGDGQTAFANPEALARAGRALGYALRIEDVSISEGGTEVTIKNCGAATVPYHMYAS
                     FGSVSSTDSLKGLRSDDSRTLRVAVADPDPATFILTSPVLLDGQVIPYSVE"
BASE COUNT          602 a          646 c          747 g          737 t
ORIGIN      
        1 tcagacaaaa tggaatcatc ggctgagcgt ccttccggca acttctcgag tcgtaatctg
       61 gaggccggtc tgcagactgc gggacggaag gacgttagtc ctggagaacc gcaggacgct
      121 gtccggccga cttccggtaa tccgttgccg gaagggcgcg tgcggacagc gacgggccga
      181 tgtagcggaa tcggtaacca gcagaacgtg agcgaaggta aacgcagcag aatgcgggta
      241 agcaacctgc cagtgaaaag cgtgttcgca ttcagtgcct gagtcttctg aagggtaagc
      301 tcaacgtttt gaataagctg ccccaatgag cgccgggcaa gtgcaacgcg ccggagcccc
      361 taagaggccc ccctttgcga ttacatcaag gttcgcgtgt ttaccaattt tttttgcgaa
      421 ctcttcacca tcggaagtaa ggcgaaggtg catggtgcgg cagcggacgc attgatccag
      481 gagggcctga agtttgccgt cgtagataat agttccccgg tcgataacaa cgaggcggtc
      541 gcacacgtcg cgaatgtcgt ccagatcgtg cgaggtaagg agtatggtag tgcctcgctc
      601 ccggtttatt ttttttagaa actcgcggat acgttcccgc acggcaacat caaggccaat
      661 agtcggttca tccaaaaaaa caactgatgg gtcatgcaaa aaagcaaggg caagatctgc
      721 ccgcatgcgc tggccaagag agagctttcg ggcagaagaa cccataaagg gtgtaaggcc
      781 gagcatttcg gaaagccatg caaggttttc tttgtagcgc ttggggggaa tggcgtagat
      841 gtccctgagc agggtaaaag attcagatac gggaatatcc caccacaatt gcgtgcgctg
      901 accaaacact acgcccatgt gcttggtgtt ctccagtcgt tcgcggtatg ggtccatgcc
      961 gtttatctgg atctgtcctc cggaaggttt aagaataccg gtgagcatct tgatggtggt
     1021 tgattttccc gcgccgtttg gtccaacata cgcgaccgtt tctcccgggt ttatggaaag
     1081 gtgcacgtcg ctaacggcct taatctcggt aaagactggt ttgataaagt gcttaagggc
     1141 tccagcgagc cccgcttcct taaccgcgtt ttgatacgtt ttagataaac cttttgcttc
     1201 tattattgcc ataatggtgg gaagtatatc cctgtgtatc cccttgataa agggggtaag
     1261 cacttgtaat tatggtattc gcgctctgat gggctatact agacctatga aaacacacgc
     1321 gtttgtttgg tatgccgtca cggcggccag tatgtgtctg tggctttcct gctcttcttc
     1381 cgattcggga gattctttag atctctctcc ttctttatcc aaagggcctc ctcctctgtg
     1441 cggtatcgtt ttgtggcaga ctaatccaat gataacacta tacaaggact cgatctcgct
     1501 tgagttctcg tacttttact actctgatgt tgccagtgac gatccttctg gcgggtatcg
     1561 gtatgactgg acgaaggttg ataccttcct tacgcaggct aaggggcggg gccatcatgg
     1621 cattgtccgt tttcgggata ccgatccgga acttgggctt gttgataaaa gtcttccgga
     1681 ttccttgtgg gtacataccc gtcaggccaa ctatgacgaa ggtatagccg gggcggcaaa
     1741 aaagagagtg gttttccccg attggtcaaa tcccgactta cctgccttta ttagttcttt
     1801 ctataaaaaa atggcggaac ggtatccgga ccaatcaacc ggtcttggat atgtagaggt
     1861 gggctttggt ttttgggcgg agtatcatat agactttgat aatctgagta acttttccca
     1921 tgcggatgct gatacgatgg cagaagccct gggccgcatt ttcccatccc gcgctgatca
     1981 ggtttctatc cttaccgagg tgtcccgatc cttcgtgaat gttccctggg gaatctcggt
     2041 ggacgcagga gataccgatt ttggtccgta tgcggctgat cttcccgtca atgccccggc
     2101 ctttggcatt tttgatgatt cgcttcttca tggggagtgg aatgatacga actgggaaaa
     2161 ctggaagttt tttgaagacc gtataacggc gggaatgaac ggtggtgagt ttagttatta
     2221 taccgatttc gatcaagcgc atgccctgga ttccgatgga ccgcatggcc tttctcttgc
     2281 ggaagtagcg cggcgctgta acttgtcgtt tgtcattggc gacggtcaaa cagcatttgc
     2341 caacccggaa gcattagccc gagccggccg ggcccttggc tacgcgctca ggattgagga
     2401 tgtttcgatt tctgagggtg gaactgaggt taccatcaag aactgcggag cggcaacggt
     2461 tccctatcat atgtacgcat cctttggctc tgttagttcc acggattcgt taaagggttt
     2521 gaggagtgac gatagccgta cccttcgcgt ggcagttgcg gaccctgatc cggctacctt
     2581 tatccttact tctccggtac tacttgacgg acaggttatt ccctattcgg ttgagtgata
     2641 ttttagcttg gcggacaagc tctgtattgt tccgatttga gtctttttta gatccttcgt
     2701 attatgacat tattcttgat taccgtaaaa gg
//
LOCUS       DAOQXQ010000173         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_40870_length_2713_cov_2.665538, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000173 DAOQXQ010000000
VERSION     DAOQXQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40870_length_2713_cov_2.665538"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKH81_03710"
     CDS             <1..322
                     /locus_tag="PKH81_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ97174.1"
                     /translation="LVDAHANVNHVSLDRGYSPLMDAVQKGDEVICRILLEHGADPDL
                     KSKDGQTALVIATGRGDISLCRLLYASGANPDITDNLGMSARKYADLFKNAELTELFN
                     TPRT"
     gene            336..1436
                     /gene="murG"
                     /locus_tag="PKH81_03715"
     CDS             336..1436
                     /gene="murG"
                     /locus_tag="PKH81_03715"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNQ97175.1"
                     /translation="MRSIVFTGGGTGGHIFPGLAVIEQLDRREHQIRWIGSSSGIDRS
                     LVESADISFYGIPSGKLRRYVSLKNFFDLFKIVAGFFCSLVLLIRFRPDVLFSKGGFV
                     SVPPCFASRLLGIPVITHECDFSPGLATRLNARIASKILVSYPETARFFSETTRARIT
                     VTGNPVRSAFYSGDPDAGRAFLSFTDNSLPIIFFQGGSLGAVQINDLVFESLPLLLPL
                     CNIVHQTGQSTVSGAPPCIDPLVAQRYRPFPFIRGEMADVLAASYIVVSRSGANTIWE
                     CAAAGKPMILVPLEKGSSRGDQVENASYFQERGAACVLSGDQATAAQLVTLIHSLADN
                     SPTYQAMQSASSALGSHRPSQIIADIIRGYVQ"
     gene            1433..1927
                     /locus_tag="PKH81_03720"
     CDS             1433..1927
                     /locus_tag="PKH81_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNQ97176.1"
                     /translation="MTLTSFDIVLLILMLFMIIRSSIKGFVAEFFSKAAVILAIMAAI
                     LFYRPLSPYVARISGADSLTQIISFLLLFLVVYLVVKLIQQVVGKAFEGESMTNLDRA
                     LGFFLGIAEGFLLILFLLFIIKSQHFIDLSFITRDSFFSRIFEPFLSEGKGILPHLFP
                     LESI"
     gene            1949..>2713
                     /locus_tag="PKH81_03725"
     CDS             1949..>2713
                     /locus_tag="PKH81_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97177.1"
                     /translation="MFENLIDQGVCDLLRSDIQNSRLPPSMLFYGREAQGKLTCALEV
                     ARILSCERTEALWTCDCPSCVLHRALSHPDLLIIGSRDCTSEIRAAGATFLSKPQAST
                     RYLFLRSIRKLTLRFSGQTVNTDDARFARAIPHLSDIEEYLEEIDPSRPIEGDTTKLE
                     KIIKAIIDKCEKLESDSLPESIPVSQIRNASTWSRLVPYGKQKVLIVDNADRMQEGAR
                     NAFLKILEEPPEHMVFVLTTSRRGAMLPTILSRVRTY"
BASE COUNT          647 a          621 c          596 g          849 t
ORIGIN      
        1 gctcgttgat gcacatgcaa atgttaatca tgtttccctt gatagaggat attccccttt
       61 gatggatgct gttcagaaag gtgacgaggt tatctgtaga attttgcttg aacatggagc
      121 tgatccagac cttaaaagca aggatggtca aacggccctg gttattgcaa ccggccgggg
      181 tgatatcagt ttatgccgat tgctatatgc ttcaggggca aatccagata ttactgataa
      241 tttagggatg tctgcacgaa aatacgccga tctctttaaa aacgcggaat tgacggagct
      301 tttcaatacc ccgcgcacat gatacactga cacctatgag atcgattgta ttcaccggag
      361 gtggtactgg ggggcatatt tttccaggcc tcgcggtcat agaacagctg gatcgtcgtg
      421 aacatcaaat ccgatggatt ggatcttcaa gcggtataga tcgctccctt gttgagtctg
      481 cggacatctc tttttatgga atcccatcgg gtaagctcag aagatacgta agtctaaaaa
      541 atttttttga cctgttcaaa atcgttgccg gttttttttg ttctcttgta ttacttattc
      601 gttttaggcc tgatgtatta ttttccaagg gcggttttgt aagtgtacct ccctgtttcg
      661 catctcgctt attgggtatt cccgtaatta cccatgaatg cgatttttct cctggtttgg
      721 caactcgcct taacgcccgt attgcaagca agattcttgt ttcatatcct gaaacagcac
      781 gttttttttc tgaaaccacc cgcgcacgaa ttacggttac gggaaacccg gtacgatcag
      841 ctttttattc gggagatccc gatgctggtc gcgctttttt atcttttaca gacaattccc
      901 ttcccattat tttttttcag ggtggaagtc ttggggcagt gcagataaat gatttggttt
      961 ttgaatcctt acctttgctg ttaccgttat gtaatatagt ccatcaaacc ggccagtcaa
     1021 cagtatctgg agcgccgcct tgtatagatc ccttagttgc ccaacggtat agaccttttc
     1081 cattcatccg cggagaaatg gcagatgttc ttgccgcaag ttatatagtt gtatcacggt
     1141 ctggtgcgaa tactatctgg gagtgcgcag ctgcaggaaa accgatgatt ttagtgcctc
     1201 tcgaaaaagg ttcttcccga ggcgaccaag ttgaaaacgc atcgtatttt caggagcggg
     1261 gcgctgcctg tgtgctttcc ggtgaccagg ccacagctgc ccagttagtt actcttatac
     1321 acagcctggc cgacaacagt cccacgtatc aagcaatgca aagcgcatct agtgcccttg
     1381 gttcgcaccg gccttcgcag atcatcgcgg atattatcag gggatatgta caatgacctt
     1441 aacttctttt gatattgtct tgctcatctt gatgcttttt atgattattc gctcttcgat
     1501 taaaggtttt gttgcagaat ttttttccaa ggcagcggtg attcttgcca ttatggccgc
     1561 gatactcttt taccgcccgc tatcccccta tgtggcacgt ataagcggag ccgattcact
     1621 tacacagatt atctcctttt tgctcctgtt tcttgtggta tatcttgttg tcaagctaat
     1681 acaacaggtg gttggtaaag cttttgaggg tgagtccatg actaatctgg acagggcctt
     1741 aggctttttc ctgggtattg cagaaggttt tttacttatt ctctttttgt tgtttattat
     1801 caagagtcag cattttattg atctgtcttt tattaccagg gacagttttt tttcccgaat
     1861 atttgaacca ttcctcagcg agggcaaggg gattctccct catctttttc cgttggagag
     1921 catttgaaag ccaggagtaa tctgtatcat gtttgaaaat cttatcgatc agggagtctg
     1981 cgatctttta cgctctgata tacaaaacag ccgtctgcct ccgtctatgc ttttttatgg
     2041 acgtgaagct cagggaaaat taacatgcgc actggaggta gcccgtattc tttcgtgcga
     2101 gcgcacagag gctctatgga cgtgtgattg tccttcgtgt gtccttcatc gcgcactctc
     2161 tcatccagat cttctgatca tcggttctcg cgattgtacc agtgaaattc gcgctgccgg
     2221 ggcaacattc ttgtcaaaac ctcaagcctc aactcgctac ctctttctac gatctatccg
     2281 taaactaacg cttcgcttta gtggtcaaac ggtaaacacc gacgatgcac gattcgcccg
     2341 agcgattcca catctttctg acatagaaga gtatcttgaa gaaatcgatc cttcccgacc
     2401 catcgaaggc gacactacaa aactagaaaa gataattaag gctattatcg acaagtgtga
     2461 aaaactggaa tcagattcat tacctgaatc gattccagtc tcacaaattc gcaatgcttc
     2521 cacttggtct cgtcttgttc cgtacggaaa gcaaaaagtg cttattgtag ataacgccga
     2581 ccgcatgcaa gaaggcgctc gtaacgcatt tcttaaaata ctagaagagc ctccagagca
     2641 catggtgttt gtactaacta cttctcggcg tggagcaatg ttgcctacta ttctttctag
     2701 ggtcagaacc tat
//
LOCUS       DAOQXQ010000174         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_40972_length_2709_cov_3.097589, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000174 DAOQXQ010000000
VERSION     DAOQXQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40972_length_2709_cov_3.097589"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKH81_03730"
     CDS             <1..610
                     /locus_tag="PKH81_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97178.1"
                     /translation="REKAHHKNVLSGIAEAVLYAIQTPHIRRAGLASGAIMTTGIISI
                     WGYFLILEQIAFPLGLYGLMFFFFQSFSALGARLSRIISAKTGKGFEWLLILTISFVL
                     ISIALFPSPLTIVLAFLHSFCWGLSSPHFLTIINSKAREDIRATVLSTMSMISRGIYV
                     LCGPLFGMIVDKGGTGRGFFFLAIICGLCFLPTILRAVDKGH"
     gene            complement(607..990)
                     /locus_tag="PKH81_03735"
     CDS             complement(607..990)
                     /locus_tag="PKH81_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ97179.1"
                     /translation="MKSILYIGLAILALFSAASVFARSSDRKIDAKTAKKMLGDDSSI
                     ILLDTRTEGEYASGYIPNAILLPYDRIDEVSAAKIIPSKTTTVIVYCRSGRRSAIAVN
                     TLASLGYTNVYDLGGINSWPFDIVR"
     gene            complement(1046..1900)
                     /locus_tag="PKH81_03740"
     CDS             complement(1046..1900)
                     /locus_tag="PKH81_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ97180.1"
                     /translation="MKILSSRLTILSDNTSASSWQVEHGFSVLITLQGNEVEGNPSIL
                     FDVGRGALLANVHNYGIDLSTVGTIVLSHGHYDHTDALKEVIQSASLAEVYASSLILG
                     QHYSMRGGSARYIGLSDATRQVLSDLASRFQLVSGVQSLFQDSLFLADCIPRLCDFEN
                     PSPLLFSDPDCTVPDSIPDELILWFATSQGTVIVTGCCHAGLVNTCEYVRSRTANAPI
                     HAILGGFHLAGVGEVRLEKTARYLNTLGLTHLIPCHCTGAAETDILASLLPGKVIPGY
                     AGLSLSLP"
     assembly_gap    2084..2093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2252..>2709)
                     /locus_tag="PKH81_03745"
     CDS             complement(2252..>2709)
                     /locus_tag="PKH81_03745"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNQ97181.1"
                     /translation="NGVIRQFLEDVDWGELDALIVDCPPGTGDEPLSVIQLIGNADGA
                     IIVTTPQEVAAVDVSKSINFCSQLGLPIAGIVENMAGFVCPHCKEITYVFKEGGGKRL
                     SQKYGVPFLGSIPIDPAIGEAGDNGTPYVSVYLSSPVSGIYSEITRSML"
BASE COUNT          732 a          697 c          628 g          642 t
ORIGIN      
        1 ccgcgaaaag gcacaccaca aaaacgtcct ttcgggcatt gccgaagctg tcctctacgc
       61 aatacaaacc ccccatatcc gtcgcgcagg tctcgcttcg ggagcaatca tgacaacagg
      121 tatcatctct atctggggat attttttgat cttggagcag attgcttttc cgctcggatt
      181 atatggactt atgttctttt ttttccaaag tttctctgcc cttggggccc gactttcccg
      241 tataatcagc gcaaaaacag gcaaaggctt tgaatggcta ttgatcctga caatctcttt
      301 cgttttgata tcgatcgcgc tttttccctc gcctcttacc atagtacttg cctttcttca
      361 ctctttttgc tggggccttt cttccccgca ttttttaact atcatcaata gcaaggcacg
      421 ggaagacatc cgcgctacag tattgtctac catgagcatg atctccaggg gtatctacgt
      481 tttgtgcggc ccgctattcg gtatgatagt cgataagggt ggaaccggaa gaggcttttt
      541 ctttcttgcc attatctgtg gattgtgttt tttaccaaca atcctgcgag cagtagacaa
      601 gggtcattag cgcacgatat caaacggcca ggaattgata ccacccaaat catacacatt
      661 ggtataacct agcgaagcga gtgtgttcac ggcaatagct gaccttctac cgcttcggca
      721 atacacgatt actgttgtgg ttttagaagg tattatcttt gccgcggata cctcatctat
      781 gcgatcatag ggcaagagaa tggcattggg aatatacccg gatgcatatt ctccctcagt
      841 tcgggtatca agaagaatga tagaactatc atcgccgagc atctttttag cagttttggc
      901 atctatcttt ctgtccgaac ttcgggcaaa gacggaagct gcagaaaaaa gggccagaat
      961 tgcgaggcct atatacaaaa ttgatttcat ggaacacctc cctcatataa tatacaaaga
     1021 aagcgcccaa ttaactgtaa gaaagttacg gaagagacag ggaaaggccc gcatagccgg
     1081 gaataacctt tcccggtaat aacgaggcaa ggatgtctgt ttcggcagct ccagtacaat
     1141 gacagggtat tagatgcgtt agcccaagag tattcaaata gcgagcggtt ttttcaagcc
     1201 gaacttcccc cacccctgca agatgaaagc ctcccagaat ggcatgtata ggagcgttcg
     1261 cggtccgaga gcgcacatac tcacatgtat tgaccaaacc agcatgacaa catccggtta
     1321 ctattaccgt accctgcgaa gtggcaaacc acagaataag ctcatctggt attgaatcgg
     1381 gcaccgtgca gtcggggtcg gaaaaaagaa gaggactggg attctcaaaa tcacaaagac
     1441 ggggaataca atctgcaaga aacagagaat cctgaaaaag agattgcact cccgaaacca
     1501 gttggaaacg ggaggcaaga tcagaaagca cctgacgagt tgcatccgac aaaccgatat
     1561 acctggccga tccaccgcgc atactatagt gttgtcccag aattagagaa gaggcataca
     1621 cctctgccaa agaagcgctt tggataacct ctttaagcgc atcagtgtga tcgtaatgac
     1681 catggcttag aacaatcgtc cctacagtcg acaaatcaat cccataattg tgaacatttg
     1741 ctagaagcgc accacggccc acatcaaaga ggatcgaagg attcccctcc acctcattcc
     1801 cttgaagggt aatgaggacg gagaaaccat gctccacctg ccatgatgaa gctgatgtat
     1861 tatcggaaag aattgtcaaa cgcgaagaaa ggatcttcat atcggtttaa tgatcgcagg
     1921 cattcgcacc ggtaataaga gtcccggcaa gatactgcgc aacaactgaa tcggaagaat
     1981 caggatgtgc ccctacgcag acggtaatac ctcgctcggt aaaaagatcg cgggcacgaa
     2041 cgcccatacc gccagcgata atgtgggtaa tccctttttc gccnnnnnnn nnncaaaaac
     2101 tttggcaata atcccggttc atgtggaggg gcttcgatat agtcctttga acttaccttc
     2161 ttggtttgct catccacggt aaacacatca aacccggcgc aatggccaaa atgcaaattg
     2221 agccgacctt cggctactgg aacagcaatc ttcataacat actccttgta atctctgaat
     2281 atattccgct aaccggcgac gacagataca ctgacacata cggcgtaccg ttgtctccgg
     2341 cctctcctat cgcaggatca atggggatgg aacccaaaaa gggaactcca tacttttgcg
     2401 aaagccgctt tccgccgcct tccttaaaga cgtaggtaat ttccttgcag tgcgggcaga
     2461 cgaatcccgc catgttctcg acgatcccgg cgatgggaag cccgagctgc gagcagaagt
     2521 tgatcgactt cgagacgtcg accgcggcga cctcctgcgg ggtcgtcacg atgatcgcgc
     2581 cgtcggcgtt cccgatcagc tggatcacgg aaaggggctc gtcccccgtg cccggggggc
     2641 aatcgacgat cagcgcgtcg agctcgcccc aatcgacgtc ctccaggaac tgcctgatga
     2701 cgccgttct
//
LOCUS       DAOQXQ010000175         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41060_length_2705_cov_3.804151, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000175 DAOQXQ010000000
VERSION     DAOQXQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41060_length_2705_cov_3.804151"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKH81_03750"
     CDS             complement(<1..259)
                     /locus_tag="PKH81_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HNQ97182.1"
                     /translation="MSTLSKKIDFAIIMGVKNANPNGDPLNGNRPRTDYEGLGEISDV
                     CLKRKIRDRLVERYVSLKNEEDKKGQAVFVQSDDRKIDGETS"
     gene            complement(285..2336)
                     /gene="cas8c"
                     /locus_tag="PKH81_03755"
     CDS             complement(285..2336)
                     /gene="cas8c"
                     /locus_tag="PKH81_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNQ97183.1"
                     /translation="MSWIQKLYETYDACVSNDSIPDIEDLCPVGYSLQNAHIEVVINS
                     QGNFRRASVVQKDNNPKTLIPVTESSAGRTSGEDPHPLCDSLQYCAGDYAQFGDREPY
                     FESYSERLELWSKSIFSHEKVKAVYSYISKRRLIKDLIEKELLAIKDGKLAIVEKTND
                     KKQVKITAPNTNDQPITIDSKELHPIMRLLAFQKLSNGKEEKNQAKIFIRWKVESDES
                     PCSETWKDTTLFEQWAKYSDSLEAEKGFCCVTGERDTVIAQKHPAKLRSGKDGAKLIS
                     SNDTSGFTFRGRFLSVNEAASVSSIVTQKAHSALRWLIGRKQAFRSGDQVIVSWATTG
                     KEIPNPCDNSWDFLGEDLQANPSSSQAYDAGHSFALRFNKKLEGYKATIADTENIVVM
                     GLDSATPGRMAITFYRELTGSEFLERIEKWHSDFTWQQNFGKDKEDKKRTILFEGSPA
                     PRDIAWCAYGSKVEGKNGVKLLNATVERLLPSIIDGRPVPRDLVEQCVRRASNRVSFK
                     KEKNGYQPEFEKCLGIACSLYKGSHIERGYRMALEKDRCSRDYLYGRLLAVAEKIESM
                     ALFFAKESRDTTAARLMQRFADRPYSTWRTIETSLMPYMARINAKRPGLLTGYKELID
                     EICCRFLNDEFKQDHRLSGEFLLAYHCQRKWLNEHKREKGQWVLKSTDDHEEQEPDIE
                     E"
     gene            complement(2333..>2705)
                     /locus_tag="PKH81_03760"
     CDS             complement(2333..>2705)
                     /locus_tag="PKH81_03760"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HNQ97184.1"
                     /translation="HFAIAKRMLERGGRQDIFLGTRDCQGYVEPCVFGEGKGAYDRDV
                     EIGYGLMFHGFDYPDETGIDELHARFWYPTLKKGVLTFDHPENCKPDLRKFVRKMSPK
                     TFSTGNVKSVEIEAEELGVVS"
BASE COUNT          694 a          654 c          501 g          856 t
ORIGIN      
        1 ggcttgtctc accatctatc ttccgatcat cggattggac gaatactgcc tggccttttt
       61 tatcttcttc attcttcaat gacacataac gctcgactaa acgatcccgt atcttccgct
      121 tgagacacac atcggagatc tcacctagtc cttcgtaatc agttcggggg cggtttccat
      181 ttagagggtc tccattggga tttgcattct tcacgcccat gataattgcg aaatcgattt
      241 tcttgcttag agtagacata tccatttctc ccctttatgt aatgttattc ttctatatct
      301 ggttcctgtt cttcatgatc gtcagttgat ttcaacaccc actgaccttt ttctcgttta
      361 tgttcattca accatttccg ctggcagtga taggccagca ggaattcccc gcttaatcga
      421 tgatcttgct tgaactcatc attcagaaac cggcagcaga tttcatcaat caactcttta
      481 tagcctgtga gcaagccggg cctttttgca ttaatgcggg ccatgtatgg cataaggctt
      541 gtctcgatcg tgcgccatgt cgaatacggt ctgtcagcga accgctgcat cagtctagct
      601 gccgttgtgt cccgcgattc cttggcgaag aagagcgcca tagattcaat tttctccgca
      661 accgcaagca acctgccata caggtagtca cgggaacatc tatctttttc aagtgccatt
      721 ctgtaccccc tttctatgtg agatcctttg tacagcgaac aagctatgcc taagcatttt
      781 tcaaattcag gttgataccc attcttttcc ttcttgaaac taactctatt agaagcacgg
      841 cgaacgcatt gctcaacaag gtcgcgtggc actggcctac cgtcaattat tgaaggaaga
      901 aggcgttcca ctgtagcatt gagaagctta acaccatttt tgccttctac cttgctgcca
      961 tacgcacacc aggcgatatc ccttggtgct ggcgaaccct caaaaagaat cgtcctcttt
     1021 ttatcttcct tgtctttgcc gaagttctgc tgccaagtaa aatctgaatg ccatttttct
     1081 atgcgctcaa gaaattctga accggtcagt tcccggtaaa aggtaatcgc catacgacct
     1141 ggtgttgctg aatccaagcc catgacaaca atgttttctg tgtcggcaat tgtagcctta
     1201 tacccctcaa gtttcttatt aaaacggagt gcaaatgaat gtcctgcatc ataagcttga
     1261 gatgaagaag gattggcttg taagtcctct cccagaaaat cccaggaatt atcgcatgga
     1321 tttggaattt cttttcctgt tgttgcccaa gaaacaataa cttgatcgcc actgcggaat
     1381 gcctgcttac gacctataag ccagcgaagt gcgctatgtg ccttttgtgt aactatcgaa
     1441 gagacagaag ccgcttcatt gactgataga aatctaccgc gaaaagtaaa gccgcttgta
     1501 tcattcgatg agatgagctt agcaccatct tttccactgc gaagttttgc aggatgtttt
     1561 tgggctataa ctgtatctct ttcaccagtg acacaacaaa accctttttc tgcctcaagg
     1621 gaatctgagt actttgccca ttgttcaaat aaagtagtat ccttccatgt ttcggaacaa
     1681 ggagactcat cagattcaac tttccaacga ataaaaatct ttgcttggtt tttttcttcc
     1741 ttcccattag ataatttttg aaaagcaagt aatctcatta ttggatggag ttcttttgaa
     1801 tctatagtga tgggttggtc atttgtattt ggtgctgtaa ttttaacctg ttttttatca
     1861 ttagtttttt caactatcgc aagtttgcca tcttttatag caagcaactc cttttcaatg
     1921 agatctttga tcaaacgccg ttttgatata tagctataaa cagctttaac cttttcatgt
     1981 gaaaagattg attttgacca taactctaat ctttctgaat aactttcaaa atagggttct
     2041 ctatccccaa attgtgcata atcacctgca caatactgaa gcgaatcgca aagtggatgt
     2101 gggtcttccc cgctcgttct ccctgctgaa ctttcagtaa ccggtatcaa ggtttttgga
     2161 ttgttatctt tttgaactac cgaggctctt ctgaaattac cctgagagtt aataactact
     2221 tcaatgtgag cattctgtaa ggaataccct acgggacata aatcttcaat atccgggatg
     2281 ctatcgttac tgacacaagc atcataagtc tcatatagct tctgtatcca actcatgata
     2341 ccactcccag ttcctcggct tcaatctcca ccgatttaac atttcctgta ctaaaggttt
     2401 ttggtgacat ttttcgtaca aatttacgta gatcaggctt gcaattttct ggatggtcga
     2461 aagtcaatac gcctttcttc agggttgggt accagaaccg ggcgtgcaac tcatctatgc
     2521 ctgtttcatc agggtaatca aacccatgaa acatcagtcc ataaccaatt tcaacatccc
     2581 gatcataagc acctttacct tcaccgaaaa cacatggttc cacatacccc tgacaatcac
     2641 gcgttccgag aaaaatatcc tgtctgcctc ctctttcaag catccgcttg gcaatggcaa
     2701 agtgc
//
LOCUS       DAOQXQ010000176         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41122_length_2703_cov_3.121601, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000176 DAOQXQ010000000
VERSION     DAOQXQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41122_length_2703_cov_3.121601"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKH81_03765"
     CDS             complement(<1..355)
                     /locus_tag="PKH81_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ97185.1"
                     /translation="MNHILSSLFANASITLPITKESKIVILSDLHMGSGGRSDDFAKN
                     ARLVHDALKKFYLPGGYILILNGDIEELLRNRLRDIEDAWEDIYGLFSEFRKAGRLYR
                     LIGNHEIVPGPDGDVL"
     gene            complement(420..1289)
                     /locus_tag="PKH81_03770"
     CDS             complement(420..1289)
                     /locus_tag="PKH81_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97186.1"
                     /translation="MNTIRGIVVTLSIGCALFQCIACSSWKRESSTSKVSDRAYTAIV
                     TDNGVGCSVGYSGGVEYTTDGGHTWIAGLNRSVCLFGVEITGKGTTFATGNQRNFVRS
                     EDGGAHWSRMENIGAGQGKSISFCDEQTGWASSSLWAGCTSDAGSSWKALALPSEVKV
                     VETVCCIGQDSGIIAGADGRLFRTDDGGASWLELTSPFARKDDQWQAQFGKTTQGLSL
                     RFSGLQGIAAAIGTFKGTGVLMIRETVDGGKTWSKVQRFPFETVPLSVFTDLQCNVSV
                     FGSDRTVTLFSRK"
     gene            complement(1371..2273)
                     /locus_tag="PKH81_03775"
     CDS             complement(1371..2273)
                     /locus_tag="PKH81_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ97187.1"
                     /translation="MEIVTTGLVHYTGFSLPITVSESSLVPPKGYYGVLCLEEGSIAL
                     HEEKTIRYLHAPATIICGDNHQLTRIVSSESARIQSLVFRIGAINSTLPGESGLIYSH
                     DGASLVFLRPFLNIPLPGYECRTLSPSSHLTVSLLFRRLQKELLGEQGPFWPCMSRSY
                     LLELLLLLERERYTSGKAETLAIAGNGPLNEILEYLHTHYADKISLDSLAAHFATNRT
                     SLNKRFRHTLGVSAIAYLTQVRIEVASAMLRNTLLSIQEIALRTGFTDESYFSRVFRQ
                     KMGESPLAFRSKFPDPYGPVTDIA"
     gene            2350..>2703
                     /locus_tag="PKH81_03780"
     CDS             2350..>2703
                     /locus_tag="PKH81_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNQ97188.1"
                     /translation="MSPSLFIAVLTFSLVSAFTPGPNNLLALASGANWGYRRTLPHVF
                     GVAVGYAIMLALMGAGLGSLFKAAPIAQEILKWLAIAYLTFLAWKIATSTGIDDSSTS
                     KESKPVLSRKTSFCSG"
BASE COUNT          756 a          687 c          607 g          653 t
ORIGIN      
        1 gcaatacatc tccatcagga ccgggaacaa tttcgtgatt gccaatcagg cgatacaacc
       61 ttcccgcttt acgaaactcg ctaaacaagc catagatatc ttcccacgca tcctcaatat
      121 ccctgagccg gttacgtaaa agctcttcaa tatcaccatt aaggatcaag atatacccgc
      181 caggaagata gaatttcttc agagcgtcat gtaccaggcg ggcattcttt gcaaaatcgt
      241 cagagcgacc tcctgatccc atatgtaaat cgctcagaat tacaatctta ctctcctttg
      301 ttatcgggag agtaatcgaa gcattcgcaa aaagcgaaga cagaatatgg ttcatgatct
      361 acactatgga gacgccgcat cagaagatga ttaacacatg gttagaattc tgttattgct
      421 tattttcggg aaaagagcgt taccgtgcga tctgagccaa agaccgacac attacactgc
      481 agatcggtaa aaaccgaaag cggtactgtt tcaaacggaa atcgttgtac ttttgaccag
      541 gtttttcctc catcaaccgt ttcccgtatc atcaatacgc cagttccctt gaaggtacca
      601 atcgcggcgg cgatcccctg tagtccgcta aaccgcaggg aaaggccttg ggttgtttta
      661 ccaaattgcg cctgccactg atcatccttg cgcgcaaagg gacttgtcaa ttccagccag
      721 gaagcgcccc catcatcagt tctaaatagc ctgccgtctg cgcccgcgat tatccctgag
      781 tcttgcccga tacaacacac tgtttcaaca acctttacct cggaaggaag cgcaagtgct
      841 ttccatgagg atccagcatc agaagtacag cctgcccata gactggaaga ggcccatcca
      901 gtttgctcgt cacagaacga gatggatttc ccttgccccg cgccaatatt ctccattcga
      961 ctccagtgag cgccgccatc ctcagatcgt acaaaattcc gctgattccc tgtggcaaaa
     1021 gtagttccct tcccggtaat ctcaacacca aacaaacaaa cggaacggtt cagtccagca
     1081 atccaggtgt gccctccatc tgttgtgtac tcaacgccac ctgaataccc aactgaacag
     1141 ccaaccccat tgtctgtaac gattgccgta tacgctctat ctgatacctt gcttgtggag
     1201 ctttcccttt tccaggatga gcaagcaata cattgaaaca gcgcgcaccc aatagacaaa
     1261 gttacgacga taccacgaat tgtattcata caaaacctcc ttacacagga gtaagtgtat
     1321 acacagtaga aggggctcag gaatgaacta tccgcaacaa agatggtact ttacgcaata
     1381 tcggtgacag gcccataggg gtcgggaaat tttgaacgaa aggccaaagg agactctccc
     1441 attttttggc gaaaaaccct actaaaataa gattcatcgg taaagccggt acgaagggca
     1501 atctcttgta tcgacaaaag ggtattgcgc agcattgcag aggccacctc tattcgaacc
     1561 tgtgttagat aggctattgc cgaaacgcca agggtgtgcc tgaatcgctt gtttaggctt
     1621 gttctgttag tggcaaaatg ggctgcaaga gaatcaaggc tgattttatc cgcgtaatgg
     1681 gtatgtaaat actcaagaat ctcgttaagc ggtccattgc ccgcaatcgc aagagtctct
     1741 gccttgccag aggtatagcg ctctcgttca agaagcagta aaagctccag caaatacgag
     1801 cgggacatac agggccaaaa gggtccttgc tctccaagca gttccttctg taagcgacga
     1861 aacaacagag aaacggtcag gtgagatgaa ggactaagtg tgcgacactc atatcccgga
     1921 agcggaatgt tcaaaaaggg gcgtaagaaa acaagggagg ctccatcatg ggaatagata
     1981 aggcctgatt cccccgggag ggtgctgtta attgcgccga tgcgaaatac aagagactgg
     2041 atacgggcgg actcagagct cacaatacgg gtaagctgat ggttgtctcc acagattatt
     2101 gttgccggcg catggagata ccgtattgtc ttttcttcat gaagggcgat cgatccctcc
     2161 tcaagacaca gtacaccgta ataacctttt ggcggtacca gagaagattc cgatacagtt
     2221 ataggcaggg aaaaccctgt ataatgaaca agtccagttg ttactatttc cataaaactt
     2281 aagaaatctt acttgattcg aaccgacaat tcaagacaca atacccctgg ccatataagg
     2341 agcacaagta tgagtccctc actttttatc gcagtgttaa cattctccct tgtctcggcc
     2401 tttacgccgg ggccaaacaa tcttctagcc ctggcatcgg gggcgaactg gggatatcgg
     2461 cgtacgcttc cccatgtgtt tggagttgct gttggctatg ctattatgct cgccctgatg
     2521 ggggccgggc ttggtagtct ttttaaggca gccccgattg cccaggaaat tctaaaatgg
     2581 ctggccattg cctaccttac ctttcttgcc tggaaaatcg caacctcaac aggaatagac
     2641 gacagtagta cgtctaaaga atcaaagcca gtgctctcaa gaaagacctc tttttgttct
     2701 ggc
//
LOCUS       DAOQXQ010000177         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41210_length_2700_cov_2.965974, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000177 DAOQXQ010000000
VERSION     DAOQXQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41210_length_2700_cov_2.965974"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..641
                     /locus_tag="PKH81_03785"
     CDS             18..641
                     /locus_tag="PKH81_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNQ97189.1"
                     /translation="MSRKTAFKVNQKIVYPSQGVGKILEIKERSFKDEMVLYYVIYLE
                     VSDMTVMVPVLKAEELGIRPIVSQDEARAALDLISQEFEPIPSDRKLRYQINLDLLKK
                     GSVIDIATIVRSLYHRSKVKELPILERKLYDSARKLLEDEITFSLDLPREEVEALIHT
                     HLEPFGDSEPTPVKSSAAAVEDDDDEDFSDNELVEDDDSQEADDSED"
     gene            645..1361
                     /locus_tag="PKH81_03790"
     CDS             645..1361
                     /locus_tag="PKH81_03790"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HNQ97190.1"
                     /translation="MTHTLVITAAGGSSRMDAMGKKEYLTLREEAGGRVSVLSEALYT
                     FISTGLFSLAMITVPADGEELAREVLAQDPRIAEAARTYNCNIFFTEGGASRQDSVRR
                     GLESLLLRTGSSRITPSTVLIHDGARPWVPADLIERVLSATVTHGAAVPTIETVDTQK
                     EVDQNGKIVRHLDRSRVFAVQTPQGFRFTELLEAHRKAAGDGKTYTDDTEIWGAYAGE
                     VFSCAGDRANRKITFQGDLP"
     gene            1358..1831
                     /gene="ispF"
                     /locus_tag="PKH81_03795"
     CDS             1358..1831
                     /gene="ispF"
                     /locus_tag="PKH81_03795"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNQ97191.1"
                     /translation="MIRAGLGWDLHRLVEGRPLIIGGVTIPFDKGEAGHSDGDVLLHA
                     IIDALLGAAAMGDIGEFFPPSDNRWKDVSSIDLLLQVREKLYASGWTIENIDCVIALE
                     TPKFLPWREAVRQKIAEALQIGLDQVFVKAKTGEGLAPIGTSEAVSAQASCLLSR"
     gene            complement(1848..>2700)
                     /locus_tag="PKH81_03800"
     CDS             complement(1848..>2700)
                     /locus_tag="PKH81_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97192.1"
                     /translation="DELKTNLEANIATLKDEISRLDVYREAMSTLEGQYGKVRKLEEE
                     ANQKMSRFMAEKKRIDILETDFSKLLALSDSIDRKISELTITNDDLQQYQVQIRRFEE
                     SIAEVNNRYERLEKKSLVLDQTVDGVDRAFENLKALETSLASWRADMEGIPADLESIK
                     DRLSTLLDNKEKTDAIIEHLDSLDSVLTDVEQRTEKMQGAREWLARTETRLEEISRQS
                     QDQLKLLGDLLKEDGGAKKTKGAPPIGIRENVVKLAHQGWKVDEISRALHLSRGEVEL
                     ILELPQK"
BASE COUNT          648 a          684 c          677 g          691 t
ORIGIN      
        1 tggtatagta ggtcagtatg agtcgtaaaa ccgcctttaa ggtaaaccag aaaatcgtgt
       61 atcccagcca gggtgtcggc aaaattcttg agataaaaga gcgttcgttt aaggacgaaa
      121 tggtcctcta ctatgtcatt tatcttgaag tgtcggacat gacggttatg gtcccggtac
      181 taaaggccga ggagcttgga attcgtccca ttgtttcgca agacgaggcc cgagcagccc
      241 ttgacctgat ctcccaagaa ttcgagccaa tcccttccga ccggaaattg cgatatcaaa
      301 taaacctgga tcttcttaag aagggcagcg ttatcgatat cgccactatc gtcagatcct
      361 tatatcatcg aagtaaggta aaggaattgc ccatccttga gcgtaagctc tacgattcgg
      421 cacgcaaact ccttgaagat gaaataacct tctcgctcga tcttccccgc gaagaggtag
      481 aagcccttat tcacacgcac cttgaaccct ttggcgattc tgaaccgacc ccggtaaaaa
      541 gttccgccgc tgcagttgaa gacgacgatg atgaggattt ttccgacaat gaactggttg
      601 aagacgatga ttcccaggaa gctgacgaca gcgaagactg acagatgacg cacacccttg
      661 ttattaccgc ggcaggcggt tcttcccgca tggacgcaat gggtaaaaaa gaatatctaa
      721 cccttaggga agaagcgggg gggagagtca gtgtcctttc ggaagccctc tataccttta
      781 tatccaccgg cttattttcg ctggcgatga ttaccgttcc ggccgacggc gaggaactcg
      841 cccgtgaggt cctggcccag gatcccagaa tagcagaagc cgcacgcacc tataattgca
      901 atatcttttt taccgaaggc ggggcctccc gtcaggattc ggtcaggcgc ggtcttgaat
      961 ccctgttgtt acgaaccgga tcaagccgca tcaccccatc aaccgtcctt attcacgatg
     1021 gggcccggcc atgggtacca gccgatttga tcgaacgagt cctctccgct accgtaaccc
     1081 atggggcagc tgtcccgacg atagaaacgg tagacactca aaaagaagtt gatcaaaacg
     1141 gcaagatagt caggcatctg gatcgctccc gcgtttttgc cgtacaaacg ccgcaggggt
     1201 tcaggtttac cgaacttctt gaagcccatc gcaaggctgc aggagacggg aagacctata
     1261 cagacgatac cgagatatgg ggcgcctatg caggcgaggt ctttagctgt gcaggcgaca
     1321 gggctaaccg aaagattacc tttcagggag accttccatg atacgagccg gccttggctg
     1381 ggacctgcac cgcttggtag aaggtcgtcc gcttatcatt ggcggggtaa ccattccctt
     1441 tgataaggga gaagcaggac actctgatgg agatgtcttg ctccatgcca ttatcgatgc
     1501 gctccttgga gcggctgcca tgggcgatat cggggagttc tttccccctt cagacaaccg
     1561 gtggaaggat gtatcctcta ttgacctatt actacaggta cgagagaagc tgtatgcaag
     1621 cggttggact attgaaaaca ttgattgcgt tatcgccctt gaaacaccca aatttttgcc
     1681 ctggcgcgaa gctgttcgcc aaaaaattgc agaagcatta caaattggcc ttgatcaggt
     1741 gtttgtaaaa gctaagaccg gagaagggct cgctcccatc ggtacctcag aggctgttag
     1801 cgcacaggca agctgtttac tatcccgata agggcactac cttcatctta tttttgcggt
     1861 aattccaata tgagctctac ctctccgcgg gagaggtgca gagccctcga tatttcgtct
     1921 accttccatc cctgatgggc aagtttaact acattttcgc gaattccgat aggaggcgct
     1981 ccctttgtct tcttggctcc cccgtcttcc ttaagaaggt ctcccaagag cttaagctgg
     2041 tcctgggact ggcgagaaat ctcttccagt cgtgtttcgg ttcgggcaag ccattcgcgg
     2101 gctccttgca tcttttcggt tcgctgttct acatctgtca gcactgagtc aagactatca
     2161 aggtgttcaa tgattgcgtc tgtcttttct ttattatcaa ggagcgtgga aaggcgatct
     2221 ttgatggatt caaggtctgc cggaattcct tccatatccg ctcgccatga ggcaagggat
     2281 gtttcaaggg ctttcaggtt ttcaaaggca cgatcgacac catcgacggt ttgatcaagt
     2341 acgagcgatt tcttctcaag tcgttcgtaa cggttattca cctcggcgat actttcctca
     2401 aaccgccgta tctgcacctg atattgttgg agatcgtcat tggtaatggt aagctcggat
     2461 atctttcgat cgatagaatc ggaaagcgca aggagcttgg agaagtctgt ttccagaata
     2521 tcgattcgct ttttttccgc catgaatcgg gacatctttt ggttagcctc ttcttcgagc
     2581 ttgcgaacct tgccatattg cccctcaagg gtagacatgg cttcacgata cacgtcgagg
     2641 cgggaaatct cgtccttaag ggtcgctata ttcgcctcaa ggtttgtttt aagctcatcc
//
LOCUS       DAOQXQ010000178         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41236_length_2699_cov_2.849849, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000178 DAOQXQ010000000
VERSION     DAOQXQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41236_length_2699_cov_2.849849"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKH81_03805"
     CDS             complement(<1..678)
                     /locus_tag="PKH81_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97193.1"
                     /translation="MFVKRSFFLVITVWLGTICSFASETAPEWTVAAESFTSEGLPEV
                     YSMYNTVLPQLILTELSRINNRLVFASERQERQVRQILDARLKLIQEKSALLRDKDKI
                     LLSNDSRYTKTKKRRDIQKRIEDKDVEIQKKGKLLAEKMGEARIVDDQEYRLTLWKKG
                     ENLYKRSESYSLAKSLYTDGISALLTGQIEDIAGYMQVTVRLDTGYGSVEQLMVSEVS
                     PYDDLDLI"
     gene            complement(668..1015)
                     /locus_tag="PKH81_03810"
     CDS             complement(668..1015)
                     /locus_tag="PKH81_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97194.1"
                     /translation="MISVLCRSVFLAKNLAQSSGMQTTVFIPDSNEVIQGKQWLATCL
                     RQHVISVVFYEPYFFVDPAYFRSLSPNTRFVIISGPGEEVATTLALSSGAVAVLHKPV
                     NDGDFHGVLDFVC"
     gene            complement(1012..2143)
                     /gene="prfB"
                     /locus_tag="PKH81_03815"
     CDS             complement(join(1012..2070,2072..2143))
                     /gene="prfB"
                     /locus_tag="PKH81_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702248.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNQ97195.1"
                     /translation="MILAECKAPIASIKNEVLEIWGRLDSDAIKAKIIEKEDFSSSPG
                     FWDDPKRAEKTMSELRSLKNRVEPWEDLLREIGDLEALYELAEESNDEQMVDEIESQL
                     EQVQAKLEKLNIINLLSGEVDRNGCFLNIHAGAGGTEACDWAQMLARMYLRWAERRGY
                     KVETVDILEAEGGIKSVTYQITGEWAFGYLKGESGIHRLVRISPFDANARRHTSFTSV
                     HAFPILDDTIEVDIRPEDLRVDTYRAGGAGGQHVNKTDSAVRFTHLPTGIVVACQSER
                     SQIMNRQMAMNVLKARLYEYYREKKEQENQKFSAEKKDISWGNQIRSYVFQPYTMVKD
                     LRTRHETGNIQAVMDGDLDPFMEAYLNNSWKGLPLEGDDEGL"
     gene            complement(2173..2493)
                     /gene="cas2"
                     /locus_tag="PKH81_03820"
     CDS             complement(2173..2493)
                     /gene="cas2"
                     /locus_tag="PKH81_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNQ97196.1"
                     /translation="MFVSVILDPGGEESARQLAEILATYGFEKAQRACWETATINEQT
                     LVRLKQDIDRVTDYYDTVRLYQFPVEGLLAVTVLIKKKWKRIVVRPPVATKSASTPRP
                     AAKR"
BASE COUNT          747 a          617 c          521 g          814 t
ORIGIN      
        1 aattaggtct aaatcatcat acggagatac ttcagaaacc attaactgtt caacagagcc
       61 atacccagta tcaaggcgaa cagtaacctg catgtatcca gcgatgtctt ctatctgtcc
      121 ggtaagtaat gcggaaatac catctgtgta taaagatttt gcaagagaat atgattcaga
      181 acgcttgtac agattttctc cttttttcca caaggttaat ctgtactcct gatcatcgac
      241 aattcttgct tctcccattt tttctgcaag aagtttccct tttttttgta tctcaacatc
      301 cttatcctca attctttttt gaatatctct tctttttttt gttttggtat atcgtgaatc
      361 gttagaaaga agtattttat ctttatcccg taataaggct gatttttctt gaatcaattt
      421 caggcgagca tccagaattt gacgcacctg tcgttcttga cgttcacttg cgaatacaag
      481 acgattgttg atacgggata attctgtaag tatcaattgc ggaagcactg tgttatacat
      541 tgagtacact tcgggcagtc cttctgatgt aaagctttcg gccgcaacag tccattcagg
      601 agccgtctcg cttgcaaaac tacaaatggt acccagccag acagttatta ctaaaaagaa
      661 agaacgctta acaaacaaaa tcaaggactc cgtgaaagtc accatcatta accggtttat
      721 gtaatacggc tacagcaccc gaagacaggg ccagcgtagt tgctacctct tcaccagggc
      781 cgctaattat tacaaaacga gtattgggac ttaaactacg aaagtaggct ggatcaacga
      841 aaaaataggg ttcataaaaa acgacagaaa tgacatgctg acgtaaacaa gttgcaagcc
      901 attgcttccc ctgaattacc tcattggaat caggaataaa aacagtagtt tgcattcctg
      961 agctttgagc aagatttttt gcaagaaaaa ctgaccgaca gagcacacta atcataggcc
     1021 ctcatcatct ccctccaggg gcaatccttt ccagctatta tttaaatatg cttccataaa
     1081 gggatccaag tccccgtcca ttactgcctg aatattacct gtttcatgac gggtgcgcaa
     1141 atcttttacc atcgtatatg gctgaaagac ataagatctg atttgatttc cccatgatat
     1201 atcttttttc tcagccgaga atttctgatt ttcctgttcc tttttttctc gataatactc
     1261 gtacaaacgg gcttttagga cattcatagc catttgtcgg ttcataatct gacttcgttc
     1321 tgactggcaa gctacaacaa taccggtggg taggtgtgta aatcgaactg cagaatcggt
     1381 tttattaacg tgttggcctc cggctcctcc tgctcgatag gtatcaactc tgaggtcctc
     1441 ggggcgaata tcaacctcta tagtgtcatc aagtatcgga aacgcgtgaa cagaggtaaa
     1501 agaagtatgg cgacgagcat tagcatcaaa gggactgata cgtaccagac gatgaatacc
     1561 ggattcacct tttagataac caaatgccca ctcgccggta atctgatagg ttaccgactt
     1621 gattccacct tcggcctcaa gtatatctac agtttcgacc ttgtatccgc ggcgttctgc
     1681 ccaacgaagg tacattcggg cgagcatttg tgcccaatca caggcttcgg taccaccagc
     1741 accagcgtgg atgtttaaaa aacagccgtt acgatcaact tctccggata agaggttaat
     1801 gatattaagt ttttcaagtt ttgcttgtac ctgttcaagc tgactttcga tctcatcgac
     1861 catctgttca tcatttgatt cttcagcaag ctcatataga gcttccaggt cgccaatttc
     1921 cctgagtaaa tcttcccatg gttctacacg gtttttcaac gaacgaagct cgctcattgt
     1981 tttctctgca cgcttaggat catcccaaaa acccgggctg gaggaaaaat cctccttttc
     2041 aattatcttg gcttttattg catccgagtc aaagacgccc ccaaatttcc aaaacctcat
     2101 tcttgattga ggctattggg gctttacatt ctgcaagtat catcatggac tccttagtgt
     2161 ataatgcaat aattatcgtt tggcagccgg tctgggcgtt gaagcactct tagtagcgac
     2221 agggggtcga accacgattc gtttccattt ttttttgatt aacacagtaa ccgctaacaa
     2281 accttctacg ggaaactgat acaatcgaac agtatcgtaa taatcggtta cccgatcgat
     2341 atcttgctta agtcgtacca atgtttgctc attaatggta gcggtttccc agcaagctct
     2401 ttgggctttt tcaaatccat aagtggctaa tatctccgca agttgccgcg cggattcttc
     2461 gcctcctgga tctagtatta cagatacaaa catagatgat agaatacggg aaagaaatta
     2521 tcttgtcaaa tcatcaatta actgcgaaac tgaatatatg actggaatat cgacgcttaa
     2581 aaaaacgttt tttctctgta tgctctgtct tgtatcagcc ttttttttgg cagaagagcc
     2641 tttgacccgc ccggtaggac tagaagttcc ccctccaccg gtgatttcgg gtcttgaag
//
LOCUS       DAOQXQ010000179         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41385_length_2692_cov_3.601062, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000179 DAOQXQ010000000
VERSION     DAOQXQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41385_length_2692_cov_3.601062"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PKH81_03825"
     CDS             <1..1236
                     /locus_tag="PKH81_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ97197.1"
                     /translation="GNWVGKQALAAVGASFPIIFLLLALFTGISMGSNILIAQYQGAK
                     NTERVKKAIETTYLYTFWGSLILMAIGFFGAGPIMTLLKSPDSVKPLAVLYLKIFSLG
                     MLPFLGFNTVGGILRGLGDAKTPLYMLVLSTALNIILDLLFVIVFGWGVAGVAFATVL
                     SQGVSLVACIIYLNYKHELLRVKFTALSFDREIFNLSVRIGIPSGIQQALVALGLATM
                     TGIVNTFGTDAIAGFAAAARIESIASLPAMTIGMAISTFVGQNLGAGKPHRVRKGLRA
                     SILLSAGISALAAISFYLFGNSLIKLFNSDENVIRIGTTYLRILGPFFIVFSFMFMLN
                     GVIRGAGETFIPLITTLLAMWAIRIPAALLLSAKLGLTGVWWAYPTGWFVGSIISLIY
                     YRSGKWIDTFEKIHGKNQT"
     gene            1343..>2692
                     /locus_tag="PKH81_03830"
     CDS             1343..>2692
                     /locus_tag="PKH81_03830"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97198.1"
                     /translation="MNTYISARTESRSARSFLQGLFPNQWRGAADARFLSSRCVNALG
                     VCGLPFTAPVLQELLKIRAFEDLLQNVKKSTDSLLLCLNENVSVSAFRHSVSSISEIH
                     LPAALLQEITSILSSVLHWAGHINSEGEHVINLKFPSPGPHFYSNLLMGNRTGFDYPL
                     QTTPKSVVDRVGRGSFRSHAATQVLATRWDLRQEENGFPSNRQFYLCEAGKPFFYSAN
                     PESDCVSEGRCIHGQNVTRIEYATTCGVTISRTMFLPKQEQGLPLAVEIQRIEITNKS
                     TTPRTLRLVYTGMFGPTKPGALQEDVLYSNIIMEGGILYNDDGTVKAVSPNYFPAADK
                     EDLRFNALVIRDEKTNRFPDEFCLNYNDFVGSGTLENPAFLAQLPNKLSRKGPGFFAL
                     AAEITLKPGETGFVDNFTGLVSSKANPSFSQESLAKEVDNLLRYFKDTESVPQALRSV
                     "
BASE COUNT          675 a          668 c          604 g          745 t
ORIGIN      
        1 ggaaactggg tgggcaaaca ggcccttgcc gccgtcggtg cgagctttcc gattattttt
       61 ttactccttg ctctctttac cggaatttct atgggatcga atatcctgat tgcgcaatac
      121 cagggagcca aaaataccga gcgcgttaaa aaagccattg agacaacata cctttatacc
      181 ttttggggaa gtcttattct gatggctatt ggcttttttg gggcgggacc aattatgact
      241 cttctgaaaa gtcctgactc ggttaagccg cttgccgttc tatacctgaa gatattttcg
      301 cttggtatgc tcccctttct tggatttaat accgtaggtg gaatccttag gggccttgga
      361 gacgctaaga ccccgcttta tatgctcgtc ctttcaacag ctcttaatat tattcttgat
      421 ctgctttttg tcattgtctt tggatggggc gtggcgggag tcgcctttgc gaccgttctg
      481 agtcagggag tttcgcttgt tgcctgcatt atctacttga actacaaaca cgaattatta
      541 cgagtcaagt ttactgcact gagctttgat cgcgagattt ttaacttgag cgtccgtatc
      601 ggcattcctt ccggcataca gcaagccctt gttgccctgg gtcttgccac tatgacgggc
      661 atcgttaata catttgggac tgacgcaatc gcaggattcg cagctgctgc gcgaatcgaa
      721 agcatcgcct cccttccagc catgacgatt ggtatggcaa tctctacctt tgtgggacaa
      781 aatctgggag caggaaaacc gcatcgcgta aggaaaggac ttcgtgcgtc aatcctcctt
      841 tccgccggga tttccgctct tgcagctatt tccttttatc tctttgggaa cagccttatc
      901 aaactcttca attcggatga aaacgttatc aggatcggta ccacgtatct tcgcatcctt
      961 gggcccttct ttatcgtctt ctcgtttatg ttcatgctta atggagtaat ccgcggggca
     1021 ggggaaacct ttatccctct aattaccacc ttgttggcca tgtgggcaat tcgtataccc
     1081 gcagcccttc tcctttcagc aaagcttggc cttaccggtg tgtggtgggc ataccccacc
     1141 ggatggttcg taggctcgat tatttctcta atctattatc gctctggcaa gtggattgac
     1201 acctttgaga agatacatgg aaagaatcaa acatgatacg tctcattcaa taaaaaaaaa
     1261 tcataagcgg cataattttg gttgatttgt tgcgagattc aacatattat acaataataa
     1321 aatctctcaa gaaggattaa ccatgaatac atacatttca gcgaggaccg aatcaaggag
     1381 cgcacggagt ttcttgcagg gattattccc caaccaatgg cgaggagcgg cggatgctcg
     1441 attcctttcc agccgttgtg taaacgctct tggtgtatgt ggcttgccct ttaccgcccc
     1501 ggtactacag gaactcttaa agataagagc ttttgaagac ttactgcaaa acgtaaagaa
     1561 atcgacagac tcattactcc tttgcctaaa tgaaaatgtc tcagttagtg cctttcggca
     1621 ttctgtatct tctatatccg aaatacatct gccggcagcc cttctgcaag aaataacgtc
     1681 cattctttct tccgttctac actgggctgg acacatcaat tcggaaggcg aacatgtaat
     1741 caacctgaag tttcccagtc ccggccccca tttttactcg aatctgttaa tgggaaaccg
     1801 gacaggtttt gactatccct tacaaacaac gccaaaaagc gttgtagacc gtgtgggtcg
     1861 aggcagtttt agatcgcatg cagccaccca ggtacttgca actcgctggg accttagaca
     1921 ggaagaaaac ggatttccca gtaaccgaca attttatctt tgcgaagcgg gtaagccttt
     1981 tttttattct gcaaatcccg aaagtgactg cgtttccgaa ggccggtgca ttcacgggca
     2041 aaacgtcaca cgcatagaat acgcaactac gtgcggtgta actatttccc gaacgatgtt
     2101 tttgcccaaa caagaacaag gcttacctct tgcagtagaa attcagcgca tagagattac
     2161 caataaaagc actacgccac ggaccctgcg cctggtatat accgggatgt ttggaccgac
     2221 caagcccgga gcactccagg aagacgttct gtacagcaac atcatcatgg aaggtggcat
     2281 tctctacaac gacgatggaa cggtcaaggc ggtaagtccc aattacttcc ccgctgcgga
     2341 taaagaagac ctgcgcttta atgcattagt gattcgcgac gaaaagacca atcgctttcc
     2401 ggacgagttc tgtcttaact acaacgactt tgtcggaagt ggcaccctgg aaaacccggc
     2461 atttcttgct caacttccca acaagctctc ccgcaaaggg ccgggctttt ttgcccttgc
     2521 cgcggaaata acgctcaaac cgggggaaac aggattcgtg gataacttta ccggtcttgt
     2581 ttccagtaag gcaaatccat cttttagcca agagtccctt gccaaggaag tggacaatct
     2641 ccttcgctat ttcaaggata cagagtcagt gccacaggcc cttaggtcag ta
//
LOCUS       DAOQXQ010000180         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41415_length_2691_cov_3.198027, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000180 DAOQXQ010000000
VERSION     DAOQXQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41415_length_2691_cov_3.198027"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="pgsA"
                     /locus_tag="PKH81_03835"
     CDS             complement(<1..459)
                     /gene="pgsA"
                     /locus_tag="PKH81_03835"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNQ97199.1"
                     /translation="MKIANAFTAVRIILAPFFFLIFFLPEWMGGRFSLASVIILVPLF
                     IFMEFTDYLDGFFARRNNEVSDFGKVFDPFADVLANLTVLLTFVLAGYLNPLLYLVII
                     YREMGIMFIRMLAASNGVIIAARKGGKTKTVLYIVAAGYKEQSLFFHPYGL"
     gene            585..1289
                     /locus_tag="PKH81_03840"
     CDS             585..1289
                     /locus_tag="PKH81_03840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNQ97200.1"
                     /translation="MEHLPASSWIIFFVPSLLIHTGLGFFLIGFRKAFTIIPSGETLH
                     HINIANKITLLRISSLPTIAFLLVYNETIELRVVLPVLLIMLFMTDSFDGQVARRMKQ
                     ITRMGQMLDSISDYSLLAVISIVYYINHIVPRWMFYIIFFRLLLQALGMFVFILLHKP
                     LPVSSTWGGKITIATIMTLYVLEMIRLFTPHNLDALFIALEYMSGLIILLLSFEKARI
                     FFRQGRHVSRERKDQD"
     gene            complement(1286..2107)
                     /locus_tag="PKH81_03845"
     CDS             complement(1286..2107)
                     /locus_tag="PKH81_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNQ97201.1"
                     /translation="MAIITVSRQIASYGDEVATELATLMGYTFINRKMLEQDLLKHGL
                     TEEKLRKYDEKKPGFWASLARDRDEYFDYLREAVYDRARAGNCVFIGRGGFAILKDVS
                     GCYAVRLIAPDQVRLERLMKEFSWNEKKAQALMQESDTNRNGFHKCFFNIDHDDPASY
                     HLVLNTEHVSARDAALIISHAVSHSIDEAESSRGIVRIGELLESQRIVNHIVFDLKIP
                     VHFLEATIAGGEIVLHGVADSASVIEKVLEVARSMSQGKKVTSGISIVQDYKSYP"
     gene            2198..>2691
                     /locus_tag="PKH81_03850"
     CDS             2198..>2691
                     /locus_tag="PKH81_03850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ97202.1"
                     /translation="MTDKTVRWEPLKSTSVFSTRVFDIHEILSRSPDNQDHTFYSLKA
                     SDWVIVVPVIHSPDGEDEFLMVRQWRHGVAEESLEFPGGVMNPGEKPEEAALREMLEE
                     TGFTAKKIYHGATVSPNPAIMSNHCHFFIAEDLQDTRETHLDEDEYVTVERIPVQTVQ
                     ERMG"
BASE COUNT          799 a          625 c          536 g          731 t
ORIGIN      
        1 cagcccgtaa gggtggaaaa acaaagactg ttctttatat ccagccgcca cgatataaag
       61 aacagtcttt gtttttccac ccttacgggc tgcgatgatt actccattac tggccgcaag
      121 catcctgata aacataatcc ccatttcccg ataaatgata acgagataca gtaatggatt
      181 gagataacca gcaagaacaa aggttaataa aacagtcaaa tttgccaata catctgcaaa
      241 aggatcaaac accttaccaa aatcacttac ctcattgttt cttcttgcaa aaaagccgtc
      301 gagatagtcg gtaaactcca taaaaataaa aagaggcaca aggataatta ccgatgcaag
      361 agaaaaacgc cctcccatcc attcgggaag gaaaaaaatc aaaaagaaaa aaggggcaag
      421 aataattcgc accgcggtga aggcattcgc aattttcatc gagtaaatat cgtatttgac
      481 aaaggatatg tcaagctata ctatgggtta tggtaagaag tatcctgcgt acggtaggtg
      541 tatacctctt tctccaactc tcctttggct tcgtatttgt gtatatggag cacttgcccg
      601 cctcatcctg gattatattt tttgtaccaa gcctcttaat tcacaccgga ctcggctttt
      661 ttttgattgg ctttagaaaa gcgtttacga ttatcccctc aggcgaaacc cttcatcata
      721 tcaacattgc aaacaagatt acgcttttac gaataagctc tttaccaacg attgcattcc
      781 ttcttgtata taacgaaact attgaactac gcgtagttct gcccgtgtta cttattatgt
      841 tgtttatgac cgactcattt gatggtcagg tcgcgcgaag gatgaagcag attaccagaa
      901 tgggacaaat gcttgactca atcagcgatt atagtctact tgccgttatt tctatcgtct
      961 attacatcaa ccacatcgtt cctagatgga tgttttatat tattttcttt cgacttttat
     1021 tacaggccct gggtatgttt gtgtttatct tgttgcacaa acctctgccg gtctcctcca
     1081 cttggggagg gaaaattacc attgcaacaa tcatgaccct ctatgtactt gagatgattc
     1141 gtttatttac accacacaac ctcgacgcgc tgttcatcgc cctggaatac atgagtggtt
     1201 tgattatcct gttactttca tttgagaagg cacgaatctt ctttcggcag ggacgccatg
     1261 tatcccggga gcgaaaggat caggattaag gataggattt ataatcctga acaatactta
     1321 tcccagaggt taccttttta ccctgagaca tcgatcgagc gacttccaga accttttcga
     1381 taaccgaagc ggaatcagcc acaccatgaa ggacaatctc tcctcccgct attgtggcct
     1441 caaggaaatg aactggaatc tttagatcaa aaacaatgtg attgacgatg cgttgagact
     1501 ctagtaattc accaatgcgc acaatccccc gggaagactc tgcttcatca atggaatgac
     1561 ttacggcatg agagataatc agcgcagcat cccgagcaga aacatgctcg gtattcaaaa
     1621 ccaaatgata acttgccgga tcatcatgat cgatgttaaa aaaacactta tgaaacccat
     1681 ttcgattagt atcgctttct tgcatcaacg cttgtgcctt tttttcgttc caggaaaact
     1741 ctttcattaa ccgctcaagc cgaacctggt ctggagcaat aaggcgaacc gcgtaacaac
     1801 cgcttacgtc ctttaaaata gcaaatccgc cccgcccaat aaatacacaa ttacccgccc
     1861 ttgcccggtc atacaccgct tccctcagat aatcaaagta ttcatcccga tcacgggcaa
     1921 gagaagccca aaaacccggt tttttttcat catattttct taatttctct tcagtaagcc
     1981 cgtgtttaag gagatcttgc tcgagcattt tgcggttgat aaaggtatac cccatcaagg
     2041 tagcaagttc ggtagccacc tcatctccat atgaagcaat ctgcctggaa acagtgatta
     2101 ttgccataat gacctccttg tactgcgagc agtcgatgtt ctatctactt atatgataag
     2161 atcatacagg taatatgtca aagaaagggg cagagcaatg acagacaaga cagttcgctg
     2221 ggaaccactg aaaagcacat ccgttttttc tacccgcgtg tttgatattc atgaaatctt
     2281 gagtaggtct cccgacaatc aggatcatac cttttattcg ttaaaggcct ctgactgggt
     2341 aatcgttgtg ccagttattc actcaccaga cggagaagac gagtttctga tggtgcggca
     2401 atggagacat ggcgttgccg aagaatccct ggaattcccc ggaggagtga tgaatccagg
     2461 agagaagccc gaagaggcag ccctgcgtga aatgcttgaa gaaaccggtt ttaccgcgaa
     2521 aaaaatatac catggggcta cggtttcccc gaaccccgca ataatgtcca atcattgtca
     2581 ctttttcatt gctgaagatt tgcaagacac cagagaaaca cacctggatg aagatgaata
     2641 tgttacggtg gaacgaatcc cggtacaaac agtacaagag cgcatgggac a
//
LOCUS       DAOQXQ010000181         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41420_length_2691_cov_2.949165, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000181 DAOQXQ010000000
VERSION     DAOQXQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41420_length_2691_cov_2.949165"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..1954
                     /locus_tag="PKH81_03855"
     CDS             473..1954
                     /locus_tag="PKH81_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97203.1"
                     /translation="MEQHVEERLMSYLQKQSRPFSIDSVLRRLDEPVSRQNREDLADY
                     LRGSLLAFPEEPYNPETLWISRPALFTGKKIRIKPTRTEIAAGILIPGSRLVPFINPS
                     LLPHEFVFSFQGKPLDRTLVEVSRSDCYPLYRFYGEEYASQFLACDNSENPALFADID
                     AGDPDQFWIAGVDLKDTYWAEGFKSGDYVEVTILDWRVGLCDLSFVASEFVNQDTHKE
                     WYAALERALHQSFSEFGPGMTIDDQLVYAWFVGREELFSLAPAPMDDFFSVVESVEIV
                     PYGVETRLWFRDGEIPSPSTWAMDGFVTPMNLVDDAYAHLDLPVNGRIVDACIYDSLY
                     RREGDYSAILERLVPFTNHSAAFCIPVVERALQNRYEHLAPDYNVFVDHEGALLRHRA
                     VNLYHALVKLFHTLSVSHVSPDQVSDQGAVVLTQIMNHTISTLEALSSSSAGDSALLE
                     PMWASLEGMEESFFEIKTGIQEELPDLLKRGFSLLDDSEDDDE"
     gene            1947..2420
                     /locus_tag="PKH81_03860"
     CDS             1947..2420
                     /locus_tag="PKH81_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNQ97204.1"
                     /translation="MSDADQILAMIRSGGVFHNVSGSNPEEAFADIVQRVPLPAGVTS
                     TDLLNGLIERERLMTTSVGSGIAIPHPRNPVIQEEADERMYVCYLDRALNFDAMDGKP
                     VYVLFVILSSSSQNHLRALSRLSWLFQQESFQSVLMKKPDTNELTDVIQQYYNKE"
     gene            2428..>2691
                     /locus_tag="PKH81_03865"
     CDS             2428..>2691
                     /locus_tag="PKH81_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ97205.1"
                     /translation="MSKELDAVALSVRSLSMDAIQKANSGHPGLPLGTAELAAVLYGK
                     ILKHNPSNPSWKDRDRFVLSAGHGSMFLYSILHLSGYNVSLDDV"
BASE COUNT          635 a          619 c          674 g          763 t
ORIGIN      
        1 catttatcaa tgctcccgga gaggtaaccg tattaggcgc aaaagtgctt atttgtaccg
       61 gacataccaa tccttttgaa aaagtcaata aggatgcggt ggtatcgttg tttgaaaaag
      121 ccggattaaa aagactgagt gcgttcataa tacacctcca acggttatga ttgttgtatg
      181 tccgcttggt tctaggaacc gcgctacaca tattatttag caaatgccgt gccaactgta
      241 tctgtatgga acgattagtg agtaattctt tgtattataa gaatttaaaa agaatagtat
      301 gcaaagggat tatgggcata cctcaggaag ggaaagcatg atcggttcaa tttgccctgt
      361 aatgccactt ggagcaacag attttgtgtc ataataacac agttactagg gataaagtga
      421 ctcaggttgg gaaagactgg tataatccca aaaatcgggt atagtagtca tgatggaaca
      481 gcatgtagaa gaaagactga tgtcgtattt acagaaacaa agccggccgt tctccatcga
      541 ctccgtgtta aggcgtcttg atgaaccggt gtcccgacag aaccgcgagg accttgccga
      601 ctatttgcgc ggaagtcttc ttgcgtttcc cgaggaaccg tataatccgg agactctctg
      661 gatatctcgg ccagccctgt ttacgggaaa aaaaattcga atcaagccga ctcgtaccga
      721 gattgccgcc ggaatattga ttcccggatc gcgcctggtt ccctttataa atccatcctt
      781 gctaccccat gagtttgttt tttcttttca ggggaagccc ctggatcgca cgcttgttga
      841 ggtttcccga tccgactgtt accccctcta ccgcttttat ggcgaggaat atgcatctca
      901 atttcttgcc tgcgataata gcgaaaaccc cgcgctcttt gcggatatag atgcaggcga
      961 tccggatcag ttttggattg ccggcgttga tcttaaagac acctactggg ccgagggctt
     1021 taaaagtggc gactatgtag aagttactat tttggactgg agagttggcc tgtgcgacct
     1081 ttcgtttgtt gcctctgaat ttgtgaatca ggacacccat aaagaatggt atgcagctct
     1141 ggaaagggcc ctgcatcagt cgttttctga attcggaccc gggatgacta tcgacgatca
     1201 gttggtgtac gcatggtttg ttggccggga ggagttgttt tccctggcgc cggcaccaat
     1261 ggacgacttt ttttccgttg ttgagtcggt cgagattgtc ccctatgggg ttgaaacacg
     1321 gctttggttt cgcgatgggg aaattccctc cccgtctacc tgggctatgg acggctttgt
     1381 tacgcccatg aatcttgtgg atgatgcgta cgcgcatttg gacttaccgg taaatgggcg
     1441 gattgttgat gcgtgtattt atgattcttt gtatcgcagg gagggggact attcagctat
     1501 tttagaaaga ctggtgccct ttacaaacca ttctgctgcc ttttgcattc ccgtagttga
     1561 gcgggctctt cagaatcgtt acgaacatct tgcccctgac tataatgtat ttgttgacca
     1621 cgagggagcg ctcttgcgtc atcgggctgt taacctctat catgccctgg tgaagctttt
     1681 tcatacactg tcggtttccc acgtctcgcc agatcaagtg tcggatcagg gtgcggtggt
     1741 acttacgcaa ataatgaatc ataccataag caccttggaa gcgctctctt catcttctgc
     1801 cggggacagc gccttgcttg aaccgatgtg ggcgtccctg gaaggtatgg aagaaagttt
     1861 tttcgagatt aaaaccggaa tacaagaaga gttacccgac ctgctcaagc ggggattttc
     1921 cctattggat gattcggagg atgatgatga gtgacgctga ccagattctc gcaatgatcc
     1981 gctctggcgg ggtatttcat aatgtatccg gttcaaatcc agaggaagcc tttgcggata
     2041 tagtacagcg agtaccgctt ccggccgggg tgacatctac ggatctgcta aatggcttga
     2101 tcgagcgtga gcggcttatg accacctctg ttggttcggg tattgccatt ccgcatccca
     2161 gaaaccccgt catacaagaa gaggcagacg agcggatgta tgtgtgctat cttgaccggg
     2221 cgctcaattt tgacgccatg gacggaaagc cggtatacgt tttgttcgtt attctctctt
     2281 catcgtccca aaatcatctt cgcgcacttt cccgtctttc ctggctgttt cagcaggaat
     2341 cgtttcagtc cgttttaatg aagaaaccgg ataccaacga actgacggat gtgatacaac
     2401 aatactacaa taaggagtag tttttttatg agcaaagaac tcgatgcagt cgccctgtcc
     2461 gtgcgaagtc tttccatgga cgcgatccag aaggccaatt ccggccatcc cggtttgccg
     2521 ctcggaacag ccgagcttgc tgccgtcctg tatgggaaga ttttaaaaca caatccttcc
     2581 aatccttcct ggaaagacag ggatcgtttt gtcctttccg ccggccatgg ttccatgttc
     2641 ctttattcaa ttcttcacct gtcgggctac aatgtctctc ttgatgatgt t
//
LOCUS       DAOQXQ010000182         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41637_length_2680_cov_3.750476, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000182 DAOQXQ010000000
VERSION     DAOQXQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41637_length_2680_cov_3.750476"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="miaA"
                     /locus_tag="PKH81_03870"
     CDS             complement(<1..538)
                     /gene="miaA"
                     /locus_tag="PKH81_03870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNQ97206.1"
                     /translation="MGQRSAQPVLIIFGATASGKSDLAEYLFDTAGPLSGCAGIISAD
                     SMQVYRGMDIGTAKPSVDLRARIPHFLIDIRNPDEGFGAGDFVACADSSCLDLFNQGR
                     LPVLLGGTGFYLRHFILGLPETPPSDAAIRASLKEQCQKRGLEEMRSLLLQVDPDSAK
                     RIHQNDEYRILRALEVYQS"
     gene            complement(550..744)
                     /locus_tag="PKH81_03875"
     CDS             complement(550..744)
                     /locus_tag="PKH81_03875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNQ97207.1"
                     /translation="MIFPLEQLVEFTGNVYEITCASIRRAYQLSMVRDSSIDENDGKV
                     VSLAARQVFTKTVDYQIENN"
     gene            896..1813
                     /gene="hflK"
                     /locus_tag="PKH81_03880"
     CDS             896..1813
                     /gene="hflK"
                     /locus_tag="PKH81_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNQ97208.1"
                     /translation="MLGFAVIVALILAATGFFIVDQTEDAVITRFGKYRTTVGPGLHF
                     KIPFGVDRSYNVPTKTIQTEQFGFRTIKGGPVNTYENNITRETTMLTGDLNIVDVEWI
                     IQYRINDPRSWLFNVHQDVRRSTIRDISQSVINTLVGDRAILDVIGPERTSIESNGLL
                     LMNEQLKEFGLGVTVINVQLQNIVPPEGVQDAFEDVNRAIQDMNRLINEGKEAYNAEI
                     PKAKGEAEKLIQIAQGYSAERVNRAKGDVARFNSVYQEYRKAPEVTKRRLYLETVEAL
                     FAEKRGTILVDKKLDNFLPLKNLDKGGAQ"
     gene            1810..>2680
                     /gene="hflC"
                     /locus_tag="PKH81_03885"
     CDS             1810..>2680
                     /gene="hflC"
                     /locus_tag="PKH81_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNQ97209.1"
                     /translation="MSPFFRKLLITLAVILAFFLFANPLYIINEGEQVVVTRFGQIVN
                     SSNRPGLHIRAPLIDNVITYPRKLMSLDGDSQRIPTKENQFIIVDTTSRWRISDPVKF
                     YESVTNIDSAYSRLSDIVDSAVRTVITSSGLNEVVRDSNLINELQHQENFALGADAED
                     VQLSQITGEKVTYETISRGRRALSAEMAQTARTKVPEFGIELIDIVLRQIKYSDELTE
                     SVYNRMIKDRNQIAQTFRSTGEGKKAEWMGKLENERKTLLSSAYNNAERLKGEADAEA
                     SRIYADSYSKDPEF"
BASE COUNT          780 a          637 c          633 g          630 t
ORIGIN      
        1 agctttgata gacctcaagg gccctcagaa ttctatactc gtcattttga tgaatccgtt
       61 tggcactgtc ggggtctact tgcaagagaa gcgatcgcat ctcctcaagc ccccttttct
      121 gacattgttc cttcagtgat gcacggatag ccgcatctga tggaggggtt tcgggaagac
      181 ccaggataaa atgcctcaga tagaaaccgg ttccgccaag gagtacggga agacggccct
      241 gattaaacag gtcaagacag gaagaatcgg cacaggcaac aaagtcccct gcaccaaaac
      301 cttcgtcagg attacgaata tcgattaaaa aatgaggtat acgggcgcga agatcaacag
      361 atggcttggc agtgccaata tccatcccgc gatacacctg catggaatct gcgctgatga
      421 taccggcaca tccagaaaga gggccggccg tatcaaaaag atactcggca aggtctgatt
      481 taccggaggc agtagcgcca aagataatca gaaccggttg tgccgagcgt tggcccatgg
      541 tgttatgaat cagttatttt cgatttgata gtcaaccgtc ttggtaaaga cctgacgggc
      601 ggccagagag acgaccttgc cgtcattttc atcgatagat gaatccctga ccatggaaag
      661 ctggtatgct ctacgaatag aggcacaggt aatttcgtat acattgcccg taaactcgac
      721 gagctgttct aatggaaata tcatagatgt actatagatg tttcacacga attattcaag
      781 agaaacttgc ccctctcggc gataaaggct acattagaag caccttcagg aggttctgat
      841 gagtaatgac gctggcccgg tattccccgg gaaaaaagta acgccctcgc tggtaatgct
      901 tggtttcgcg gttattgttg ccctgatact tgccgcaacc ggttttttta tcgttgatca
      961 aaccgaagac gcggttatta cccgctttgg caagtaccgc accacggttg gaccaggatt
     1021 acatttcaag ataccctttg gtgtcgatcg cagttataac gttccgacca agaccatcca
     1081 gacagaacaa tttggattta gaacgatcaa gggtggaccg gtaaatacct acgaaaacaa
     1141 cataacccgc gaaaccacca tgttgaccgg agaccttaat atcgtagatg ttgaatggat
     1201 cattcagtat cgcattaatg acccccgctc ctggctgttt aatgtacatc aggatgtaag
     1261 aagatcaacc attcgagaca tttctcagtc tgttatcaat accctggtag gcgaccgggc
     1321 tatccttgat gttatcggtc ccgagcgtac ctctattgaa agcaatggct tactccttat
     1381 gaacgagcaa ttaaaggaat ttggccttgg ggttacggtg attaacgtac aactgcaaaa
     1441 tatcgttcct cccgagggag tacaggacgc ctttgaagat gttaaccgcg ctattcaaga
     1501 catgaaccga cttatcaacg agggaaagga agcatataac gctgaaattc ccaaggcaaa
     1561 aggcgaggct gaaaagctca ttcaaatcgc acaaggatat tcggccgaac gggtaaaccg
     1621 cgcaaaggga gatgttgccc gttttaactc ggtatatcag gaatatcgca aagccccaga
     1681 ggtaaccaaa cgccgtttgt acctggaaac cgtagaagca ctctttgccg agaaacgggg
     1741 aactatcctg gttgataaaa agcttgataa tttcctgccg ttaaaaaatc ttgacaaggg
     1801 aggcgcgcaa tgagcccatt ctttaggaaa ctccttatta cccttgcggt aatacttgcc
     1861 ttttttctct ttgcaaatcc gctgtatatc attaacgaag gggagcaagt agtcgttacc
     1921 cgctttggac agatcgtcaa ctcctcaaac cgtcccggcc tccatattcg ggcgcccttg
     1981 atagataacg taattaccta ccctcgcaaa ctcatgtccc ttgatgggga ctcacaacgt
     2041 attcccacca aggaaaacca attcattatt gtcgacacta caagtcgatg gagaatttcc
     2101 gatccggtaa aattctatga atcggtgacg aatattgact ctgcatactc ccggctgagc
     2161 gacatcgtag actctgcggt aagaacggtt attacctcat ccggtcttaa cgaagtggta
     2221 cgggattcca acctgattaa cgagctgcaa catcaagaaa actttgccct tggcgcggac
     2281 gccgaggatg ttcagctaag ccagatcact ggcgaaaaag taacctacga aacgatttct
     2341 cgaggaaggc gggccctgag tgcggagatg gctcaaacag cccgaacaaa ggttccggaa
     2401 tttggaatcg aacttattga tatcgtgctc agacaaatta aatactcgga tgaactaacc
     2461 gaaagcgtct ataaccgaat gattaaagac cggaaccaga ttgcacaaac attcaggtcc
     2521 acgggagagg gaaaaaaggc ggaatggatg ggaaaactcg aaaacgagcg aaaaacactc
     2581 ctgtcatcgg cttataataa tgccgaacgc cttaagggag aggccgacgc tgaggcttcc
     2641 cgtatctatg cagactccta cagtaaggac cctgaattct
//
LOCUS       DAOQXQ010000183         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41665_length_2679_cov_2.754573, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000183 DAOQXQ010000000
VERSION     DAOQXQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41665_length_2679_cov_2.754573"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKH81_03890"
     CDS             complement(<1..260)
                     /locus_tag="PKH81_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97210.1"
                     /translation="MKRLRTLFICVLATLSLSAQAQSGRMLNRNDQELSWSYIERIRA
                     GQNIPTTVQSLSAEEAAYYGLIPSQRPAPEAESKPFLSPRAL"
     gene            complement(257..2266)
                     /gene="uvrC"
                     /locus_tag="PKH81_03895"
     CDS             complement(257..2266)
                     /gene="uvrC"
                     /locus_tag="PKH81_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNQ97211.1"
                     /translation="MKEQTEIRKKLHAAALDAPKTSGVYLWKDETGQILYVGKAKSLK
                     NRLASYFSSTRDLKTRILVSRAHSLDYITCDNEYEALLLENTLIKQHSPRYNINLKDG
                     KTYPVIKITRETFPRIYRTRRIQEDGASYFGPFPNLNAIDTFLDFVKRHYPLRQCRVL
                     KKRATPCLYYHIGRCSAPCAGKIDAESYQSQLEEILLLLQGDNAEAVEKVRQAMKQAA
                     SDLHFEKAARLRDGLLAIEELREQNAVVDMDGESRDYIAWAAEGIMITFAVLKMRSGR
                     LVARDLYRARSLKDEEEILPEFLMAYYTRAAEVPPRIFVPTENGLTLARRWFCEGLGA
                     ETELTICRSTPSQDGEEAASPSTSQGDYRMAEEKTSQEDRRHRAAMAMAQFNAQEDAS
                     RRLRERGDFPALEELKNLLKLSTLPSRIEGFDIAHIGGRLPVASLITFHDGNPYRKGY
                     RIFRLKTTDGIIDDFASLREAVSRRYTRLLNEGEELPDLLLIDGGIGQVNAVKGILEA
                     LDLTIPVIGLAKRDEEIFLPGNSTPVTLPRRSDALRLLQRLRDETHRFATTRNQKLRT
                     QENTRLAFEDLPGIGPKKAATLLKTWGSLEALYQGLTNSEGIHKLTQTAGISADTAEA
                     IREALPALIQKRIDERHKRSASTSSHEIASLAQRATRAGNNEATP"
     gene            2320..>2679
                     /locus_tag="PKH81_03900"
     CDS             2320..>2679
                     /locus_tag="PKH81_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97212.1"
                     /translation="MKMRKIGLCVFLMCLFTAIASESKILLGGEASWPPFSVSRSIAR
                     TAGWLGYEGLCLDSRSTPTEADLYLSFDESVPSVTSGAYTIRSNSLSRVASPRSRHGS
                     GSALASTSGSALVLEGEH"
BASE COUNT          526 a          651 c          750 g          752 t
ORIGIN      
        1 ataagggctc ggggagagag aaagggtttt gactctgcct ccggggcagg cctttgcgag
       61 gggataagtc catagtatgc cgcttcttcg gcggaaagcg attgcacggt tgtggggatg
      121 ttttgtcctg cccgtatccg ttcaatgtaa gaccaggaaa gctcctgatc gttacggtta
      181 agcattcgtc ccgactgggc ctgggcactt aagctcagtg tggcgagtac acagataaaa
      241 agggtacgca atcgtttcat ggtgttgcct cattgtttcc cgcgcgtgtg gcgcgttgtg
      301 ccagcgaggc tatctcgtgg gaagatgtac tcgccgatcg tttgtgtcgc tcatcaattc
      361 gtttttgtat cagagcggga agagcttctc ttatcgcctc tgcggtgtcg gcggatatgc
      421 ccgctgtttg agtgagtttg tgtattccct cgctgttggt taagccctga tagagcgctt
      481 ccaggctgcc ccatgttttt aacaaggtcg ccgctttttt aggtcctatt cccggtaaat
      541 cttcaaaggc caggcgggta ttttcttggg tcctaagttt ttggtttctg gtcgtggcga
      601 accgatgggt ttcgtctcgc agacgctgta acagacgcaa ggcgtccgag cggcggggca
      661 gggttacggg tgtggaattg ccgggaagga aaatctcctc gtcgcgtttg gcaaggccaa
      721 tgacggggat ggtcagatca agggcctcaa ggattccttt aactgcgttt acctggccga
      781 taccgccgtc aatgagcaag aggtctggaa gttcctcccc ttcattgagc aatctggtat
      841 aacggcggct taccgcttcc cgtaaagagg caaaatcgtc gatgatgcca tcggtggttt
      901 taaggcgaaa tatccggtag cccttgcgat agggatttcc gtcgtgaaag gttatcaggc
      961 tcgcaaccgg aagtcttcct ccaatatggg caatgtcaaa gccttcgatt cgggaaggga
     1021 gtgtggaaag cttgagcagg tttttaagct cttcaagggc gggaaaatct cctctctccc
     1081 ttaagcgacg ggaagcgtct tcttgcgcgt taaactgggc catcgccatg gctgcccggt
     1141 ggcgacgatc ttcctggctc gttttttctt ccgccatacg gtagtcgccc tggctggtag
     1201 atggggaggc ggcttcttct ccatcctggc tcggtgtgct ccggcagatg gtcagctctg
     1261 tttccgctcc aaggccttcg cagaaccaac gccgggcaag agtaagcccg ttttctgtgg
     1321 gcacaaagat acgcggagga acctcggctg cccgggtgta gtaggccatt aaaaactcgg
     1381 gaaggatttc ttcttcgtct ttaaggcttc tcgctcgata caggtcgcgg gcaacaaggc
     1441 gtcccgagcg catctttaag acggcaaagg ttatcattat tccttcggcc gcccaggcaa
     1501 tgtaatcccg ggattctcca tccatatcga ctaccgcgtt ttgttcccgc aattcctcaa
     1561 tagccaaaag tccgtccctc agtcgtgctg ctttttcaaa atgaagatcg cttgctgcct
     1621 gcttcatcgc ctgtcgaact ttttcaactg cttctgcgtt gtccccttgc aaaaggagta
     1681 gtatctcttc aagctgggat tggtagcttt cagcgtcgat ttttccagcg cagggggcgc
     1741 tacaccggcc gatgtggtag tataggcagg gtgtagccct ctttttaaga acacggcatt
     1801 ggcgaagggg atagtggcgt ttaacaaagt ccaggaaggt atctatcgcg ttcaggttgg
     1861 gaaaagggcc aaaatacgaa gcgccatctt cctgtatccg gcgagtccga tagattcggg
     1921 ggaaggtttc ccgggttatc ttgattacag ggtatgtttt cccgtctttc aggtttatat
     1981 tgtatcgcgg actgtgttgc tttatgagtg tgttttctaa aaggagcgcc tcatattcat
     2041 tgtcgcaggt gatataatcc agagaatgag cgcgcgagac gagtattcgc gtcttcaggt
     2101 cgcgtgtcga ggagaaatag gatgcaagcc ggttttttag ggattttgcc tttccgacgt
     2161 agagtatctg tccggtttca tccttccaga ggtatacgcc actggttttt ggggcatcga
     2221 gagcggccgc atgcaacttt ttgcgtattt ccgtctgctc tttcattgga tttatcatat
     2281 cattctgccg atattctgat aagtacttct atgcgtggta tgaaaatgag aaaaattgga
     2341 ctttgtgtat ttttaatgtg tctctttacg gctatagcca gcgaaagcaa gattcttctt
     2401 ggtggagagg cttcctggcc ccccttttct gtttcccgga gcatagcgcg caccgccggt
     2461 tggctgggat atgagggcct ctgtcttgat tctcgaagta cccccaccga ggcagatctg
     2521 tatctttcct ttgacgagag tgtcccgtcg gtaacatcgg gtgcgtacac gattcggtct
     2581 aacagtcttt cccgtgttgc atccccccgc tctcgccacg gctctggctc tgctttggca
     2641 agtacctccg gttctgccct tgtgttggaa ggtgagcat
//
LOCUS       DAOQXQ010000184         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41818_length_2673_cov_2.775783, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000184 DAOQXQ010000000
VERSION     DAOQXQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41818_length_2673_cov_2.775783"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKH81_03905"
     CDS             complement(<1..740)
                     /locus_tag="PKH81_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ97213.1"
                     /translation="MTDRRGAQPFFSDLEAAGQNYGVLVRSSIQRGRLVEIRAPKMPD
                     GYHLYTATDIPGENRLTVMGTTIPIFTPYEINYYGEPLGILVGPDLQTVHELVAEVLI
                     ETESLESLPFSEKFNASQVVAKRVLFSGDIDVAFTKAERVVETTSATCPQDHYYAEPL
                     GVLVDFSEGRLVISCPSQWPFHVRTTVSSTLDLDPAEIVVKDGKVAGVKCQKMWLGEF
                     DRSGRRRPEATENTFVVDADQVIAAIGQS"
     gene            complement(737..1924)
                     /locus_tag="PKH81_03910"
     CDS             complement(737..1924)
                     /locus_tag="PKH81_03910"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ97214.1"
                     /translation="MSKADSIPFFKPSFTSEEEEAVLRVLRSGWLTTGPETLALEKEF
                     AAYVQSPYALAVNSATSGLLLAQQAFGVGPGDKVLTTPYTFISTATSAVHLGAEVVYA
                     DIEADGFNIDPQAIERALTQNTGIKAIIPVHIAGLPCRMSEIMDIARHYKVRVIEDAA
                     HSFPSKTKEGWAGNLADAGVFSFYATKTITCGEGGMICLNDQQASERIKRLRSHGIDR
                     TIWDRYTSNKASWLYDVTEAGWKCNLPDILSAIARVQLTRAEEFRAKRAAICEQYTKA
                     FNENPITRELIETPPEGEGHAWHLYLITLQSDRIKGTRDDFGRMLQEKGISISMHFIP
                     HFSFDWCKKRYNLNPNDFPNANRRGNNTLTLPLWPEMSKDDVNRVIEAVISTSKAMIR
                     SRP"
     gene            complement(1917..>2673)
                     /locus_tag="PKH81_03915"
     CDS             complement(1917..>2673)
                     /locus_tag="PKH81_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNQ97215.1"
                     /translation="NPVAVVENNVFGTANLLEASLESNVKRFVLISTDKAVDPVSVYG
                     ASKMLCEQLTLSYARKARDCGCESAYMFVRFGNVLGSRGSILPLFTDQIRKGGPVTVT
                     DPAMVRYFMTIPEACSLVLKTGGVGINGESYLLDMGDPVRIYDVAEQLIRFMGYEPGR
                     DIPIEIIGKRHGERLDEPLWSADEEPVKTDFPRILRLEKKKAVDDEALSQLLENLRPC
                     CIHTESKTTLFRDRSALIEALAQAIPTLEAGHE"
BASE COUNT          616 a          664 c          670 g          723 t
ORIGIN      
        1 gactggccga tcgccgcaat gacctggtct gcgtccacga cgaaggtgtt ttccgtcgcc
       61 tcgggacgcc ttctgccgct gcggtcaaac tctcccagcc acattttttg gcacttgacg
      121 ccggcgactt ttccgtcttt aaccacaatc tcggctggat ccaagtccaa tgtagaggac
      181 accgtggtac gtacatgaaa tggccattgg cttgggcacg agatgactaa gcgtccttcc
      241 gagaagtcaa caagtactcc aaggggttcg gcgtagtagt gatcttgcgg acaggttgcg
      301 cttgttgtct ctacaacccg ctccgccttg gtaaaggcta catctatgtc tccggaaaac
      361 aacactcgct tggctactac ttgggatgcg ttgaattttt cgctaaaggg aaggctttca
      421 aggctttctg tttctatgag cacttcggca actagttcat gtacggtttg aagatctggt
      481 ccgacaagga ttcccaaagg ctcaccataa taattaattt catagggggt aaagatcggt
      541 atggtggtgc ccattacggt aagacgattt tctccgggta tgtccgttgc agtatacaaa
      601 tggtacccat cgggcatctt gggcgcccga atctcaacaa gtcggcctct ttgaatcgag
      661 gagcggacca gaaccccata gttttgacca gctgcttcaa ggtcgctaaa aaaaggttgc
      721 gctccccttc gatctgtcat gggcggctcc gtatcatggc tttggatgtg gagataactg
      781 cttcgataac tcgattaaca tcgtctttgc tcatctcagg ccacagggga agggttaagg
      841 tattgtttcc ccttcggttt gcattgggaa aatcgttcgg gttaagatta tatcgttttt
      901 tacaccagtc aaaagaaaag tggggtataa agtgcataga aatgctgatg cccttttcct
      961 gaagcattcg gccaaagtcg tcccgcgttc cctttattct atcgctctgt aacgtaatca
     1021 gatacaggtg ccaggcatga ccttctccct ctggtggcgt ttctatcaat tcccgtgtaa
     1081 ttggattttc gttaaaagcc ttggtgtatt gctcgcaaat ggcggctctt ttagcgcgaa
     1141 actcttcggc tctggttagc tgtacccgcg cgatggcaga aaggatatcg ggaagattgc
     1201 atttccaacc tgcttcggtt acgtcgtaga gccaggatgc tttatttgat gtatagcgat
     1261 cccatattgt gcggtctata ccatgtgacc tcagtcgctt gattcgttcg ctcgcttgtt
     1321 gatcgttaag gcaaatcatt ccgccctctc cacaggtaat cgttttagtt gcgtaaaacg
     1381 aaaagactcc tgcgtcggcc agatttccag cccagccttc ctttgttttt gaggggaatg
     1441 aatgggccgc atcctctatt acccgaacct tgtaatggcg ggcaatgtcc ataatctcgc
     1501 tcatgcggca gggaagaccg gcgatgtgaa ccgggataat ggccttgatt cctgtgtttt
     1561 gtgtcagtgc gcgttctatc gcctggggat caatgttaaa accatctgct tctatatctg
     1621 cgtagactac ttctgcaccc agatgcacgg cgctcgttgc ggtagaaata aaggtatagg
     1681 gtgtggtaag caccttgtcc cccggtccaa cgccaaaagc ttgttgggct aaaagaagtc
     1741 cactggtggc gctattaact gcaagggcat aggggctttg tacataggcg gcaaactctt
     1801 tttccagggc aagggtttca ggcccagtgg taagccatcc ggaacgcaat acgcgaagta
     1861 ctgcttcttc ttcctcgctc gtaaaggaag gcttaaaaaa agggatgctg tcggccttac
     1921 tcatgacctg cctccagtgt cggtattgcc tgtgccaggg cctcgatgag cgcgctgcgg
     1981 tcgcggaata aagtggtttt ggattctgtg tgtatgcaac agggccgcag gttttctaat
     2041 agttggctaa gagcttcgtc atcaacagct ttcttttttt caagacggag tattcgggga
     2101 aagtctgttt ttaccggctc ttcgtcagcg ctccacagcg gctcgtcaag acgctccccg
     2161 tggcgctttc caataatttc tatgggaata tcccttcccg gttcatatcc cataaatcgt
     2221 atcagttgtt cagccacatc atagatacgc accggatctc ccatatcaag aagataggac
     2281 tccccattga tgcctactcc gccggttttt aaaaccagag aacaagcttc ggggatggtc
     2341 ataaaataac ggaccattgc gggatcggtt accgtgaccg gtccgccttt gcgtatttga
     2401 tcggtaaaaa gaggcaagat agagccgcgc gaaccaagga cattaccaaa acgcacaaac
     2461 atataggcgg actcacatcc gcaatccctt gccttgcgcg cgtaggagag ggtgagctgt
     2521 tcgcagagca tcttggatgc gccatacacc gaaacagggt caactgcctt gtctgtagat
     2581 atcagaacaa atcgttttac gttcgattca agggatgctt caagcaaatt cgcggttcca
     2641 aacacattgt tttccaccac ggcaaccggg ttt
//
LOCUS       DAOQXQ010000185         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_41930_length_2668_cov_2.695752, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000185 DAOQXQ010000000
VERSION     DAOQXQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41930_length_2668_cov_2.695752"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PKH81_03920"
     CDS             complement(<1..163)
                     /locus_tag="PKH81_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ97216.1"
                     /translation="MSDTLLEMRSITKDFPGVKALDSVSFSVKKKEIHALVGENGAGK
                     STLMNILSGT"
     gene            complement(257..1327)
                     /gene="chvE"
                     /locus_tag="PKH81_03925"
     CDS             complement(257..1327)
                     /gene="chvE"
                     /locus_tag="PKH81_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ97217.1"
                     /translation="MMKKASALFAVALAAGIILGSCAKSDAGKGTIGISMPTKSSARW
                     IADGNNMKAEFEKLGFKVDLQYAEDVVENQISQLENMITKGVNVLVIAAIDGESVTKV
                     LEKANDAKIPVIAYDRLIRNSPFVSYYVTFDNFKVGIQQASTIESALDLKNAKGPFYI
                     ELFGGSPDDNNAYFFYNGAMSVLQPYIDEGKVKIGSGQMGMDKVSTLRWDGATAQARM
                     DNLLSAFYTKQKIDAVLSPYDGISLGIISSLKGVGYGKGSTPMPFVSGQDAELPSVKS
                     ILAGEQYSTIFKDTRALAKQAVQMATALLDGKPAQVNDTKTYDNGVKVVPSYLLESTI
                     VTKDNITQALVDSGYYTADQIK"
     gene            complement(1373..2116)
                     /gene="araD"
                     /locus_tag="PKH81_03930"
     CDS             complement(1373..2116)
                     /gene="araD"
                     /locus_tag="PKH81_03930"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HNQ97218.1"
                     /translation="MAKIKSPYRELCQKAYEANMEIPARALALYTWGNVSAFDKNLGV
                     FAIKPSGVPYEELSEQSMVVIDMEGKTVEGTLRPSSDTPTHLVLYREFAIQEGRAIGG
                     IIHTHSTHAVAWAQACKPIPLFGTTHADHIQVPVPCTPYLSLEAVKGDYELETGKQIV
                     DLFRAGKAPLNPAEVTMALVGGHGPFAWGETALKAVYNGVVLEEIARMATLTIMNNPK
                     AKPLPLHIVKKHYERKHGSGAYYGQKKQV"
     gene            complement(2116..>2668)
                     /locus_tag="PKH81_03935"
     CDS             complement(2116..>2668)
                     /locus_tag="PKH81_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNQ97219.1"
                     /translation="SPMSSGLYSSQGMGDSFMEDYTYNLDPENGLNLGAHMLEICPSI
                     AASRPTIEVHQLGIGGREDPARLVFNGKKGPAVCAAVVDFGSRFRCVINEVEVIDPPK
                     AFPKLPVARVLWKPLPSLETSAEAWILAGGGHHTAFSNIVTVDMIRDWAEMTGIECIS
                     IGKDTTITAFRNELRWNAAAWRP"
BASE COUNT          633 a          634 c          655 g          746 t
ORIGIN      
        1 aggtaccgct caagatattc atcaatgtag atttacccgc tccattttcg ccaacgagcg
       61 catgaatttc ttttttcttt acggaaaagc ttacggagtc cagagctttt accccgggaa
      121 aatcctttgt aatcgatctc atctcgagaa gtgtgtctga cattcattac ccccggatac
      181 taatgtctaa aacctggaaa aaaacgcggg gctaatccgg ggagagtggt ccggattaac
      241 cccgcccgag atatttttac ttaatctggt ctgcggtgta atagccagag tcaacaaggg
      301 cctgtgtaat attgtccttg gtaacgatgg ttgattcgag cagataggaa ggaacaacct
      361 ttaccccgtt gtcgtaggtt ttggtatcgt taacctgtgc gggttttcca tccagaagag
      421 cggtcgccat ttgcactgcc tgttttgcga gggcgcgagt atccttaaaa atggtggaat
      481 attgctcgcc ggcaagaatt gacttaactg agggtagctc tgcatcttgt ccggagacaa
      541 agggcatcgg ggttgaaccc ttgccatatc ctacgccctt aagtgaggag atgatgccaa
      601 ggctaattcc atcataggga gagagaaccg cgtcgatttt ttgctttgtg taaaaagcgg
      661 aaagaagatt gtccatacgg gcttgcgcgg tagcgccatc ccagcgaaga gtagatacct
      721 tgtccatgcc catctggccg gaaccaatct ttaccttgcc ttcgtctatg tatggctgta
      781 gcaccgacat agcgccgttg tagaagaagt acgcattgtt gtcgtcgggc gatccgccaa
      841 agagttcgat gtaaaacggt ccttttgcat tcttaagatc aagagcagat tcaatggtgc
      901 ttgcttgttg gatacctacc ttgaagttgt cgaaggttac atagtagctc acaaaaggag
      961 agttccggat aagtcggtca taggcgataa cggggatttt ggcgtcgttt gctttttcca
     1021 aaacctttgt taccgattct ccgtcgattg cggcgataac caggacgttg acgcctttgg
     1081 taatcatgtt ttcaagctga gaaatctgat tctcaacaac gtcttcggca tactgtagat
     1141 cgactttgaa gccgagtttt tcaaactctg ctttcatgtt gtttccatcc gctatccagc
     1201 gggcagatga cttggttggc atggagatgc cgatcgttcc ttttcctgca tcactcttgg
     1261 cgcaactgcc gaggatgatc cctgccgcga gtgccacggc gaaaagcgct gaggcttttt
     1321 tcatcatttc ttcctcctta ataatcaaac agtccaggac cgtttgtaat agctatacct
     1381 gtttcttttg gccataatag gcgccactac catgtttgcg ctcatagtgt ttttttacga
     1441 tgtgcaaggg aagcggcttt gcctttggat tattcataat agttaaagtt gccattcggg
     1501 caatttcttc taacacaaca ccgttataca ccgctttaag cgctgtctct ccccaggcaa
     1561 aaggtccgtg tccgccaaca agggccatgg ttacctctgc cggatttagt ggcgcctttc
     1621 ctgcgcggaa gagatctaca atctgcttgc ctgtttccag ttcgtaatcg ccttttaccg
     1681 cctcaagaga tagatagggc gtacagggaa cagggacttg gatgtgatca gcgtgggtag
     1741 ttccaaaaag tgggattggc ttgcatgctt gggcccaggc taccgcatgg gttgaatggg
     1801 tgtgaataat tccccctata gcccttcctt cctgaattgc gaattcacga tagagcacaa
     1861 gatgagtcgg ggtatccgag gaaggtcgta gcgttccttc tactgtttta ccttccatgt
     1921 cgattactac catcgactgc tcgcttagct cttcgtaggg aaccccggat ggcttgatcg
     1981 caaaaacgcc aagattcttg tcaaaggcgg agacatttcc ccatgtatac agggcaagag
     2041 cccttgcggg aatttccatg ttagcttcat atgctttttg acaaagctcc cggtacggag
     2101 atttaatttt tgccattatg gtctccaggc ggcggcattc catctgagtt catttctgaa
     2161 tgcagtaatc gtggtatcct ttcctattga aatacattca atacccgtca tctctgccca
     2221 gtcacgaatc atgtctacgg taacgatgtt ggagaatgcg gtatggtggc cgcccccagc
     2281 gagaatccat gcttctgctg atgtttctag cgagggaagc ggtttccaga gaacacgcgc
     2341 gaccggcaat tttggaaaag cctttggcgg atcgataact tctacttcgt taataacgca
     2401 tctgaagcgt gagccaaaat caacaactgc cgcgcaaact gccggtcctt tcttcccgtt
     2461 aaaaacaaga cgagccgggt cttcccttcc tccgattcca agttgatgaa cttcaatggt
     2521 tggtctggat gcggcgatgg acggacagat ttcgagcatg tgcgctccca ggttaaggcc
     2581 gttttccgga tcaaggttat aggtataatc ttccataaaa gaatcaccca tgccctggct
     2641 ggaataaagg cctgaggaca tgggtgat
//
LOCUS       DAOQXQ010000186         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42085_length_2661_cov_3.102456, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000186 DAOQXQ010000000
VERSION     DAOQXQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42085_length_2661_cov_3.102456"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKH81_03940"
     CDS             <1..420
                     /locus_tag="PKH81_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HNQ97220.1"
                     /translation="YTPSDANIDKLGIPPDREVLLPELTEAEEKALSELLQTTRLNDL
                     VAGKTELRDTEAEILARELAKTYPVSLRVLKKLVYQEFYRTHLSPLYDLEFDLQLKAA
                     VDILLKENIRELLRSTKTVHELQLSGDQAVSTASSGS"
     gene            417..1232
                     /locus_tag="PKH81_03945"
     CDS             417..1232
                     /locus_tag="PKH81_03945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNQ97221.1"
                     /translation="MRQLLVDSLPAVDGTLTLRGSQYRYVRRVLRKGAGDSLECRFVD
                     GSLAFCTVLEVDDSRKSITLSVSEGGIPQVEPGYSQSASSLALDNFPRLVLLQWILKG
                     QKMDLVIRQATEAGVMDIIPVVGDRSIPERESSSRSERWLRIVREARQQSGSAVPTTI
                     HPATMSSAIVNVSNQLLSGESPTRLVFTEAPLALKSLHQYLDSGPATVVLAIGPEGGM
                     SPAELTALGTAGFNCVHFKTNILRAETCALYAVAATLSALTERDTWQLKEYIC"
     gene            1205..1948
                     /locus_tag="PKH81_03950"
     CDS             1205..1948
                     /locus_tag="PKH81_03950"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNQ97222.1"
                     /translation="MAVERIHLLKVPLDIVHEEDMEEMVLALLSRDGPQHIIMLSLWD
                     FMRARHRGEFQSLVSKAALVIPTSRALVRGARFLKKTAPVRYQSFSFIINVLGILERY
                     YKTLYLLGGNNRSLMQAEKNVRSTFPHLGIVGRFAGHYHRSMERKIQTAIVKAHPSLV
                     IMGNGIPGGQRWIYRNKSKLHSGIFFWDTDVIDIFSERKRRVSDKTFERGHEYLGQIV
                     KNPFRILRFFQYLWYNLVLLYYRLFRVYS"
     gene            1996..>2661
                     /locus_tag="PKH81_03955"
     CDS             1996..>2661
                     /locus_tag="PKH81_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNQ97223.1"
                     /translation="MKNILYLTSFTNDVAHLSYEAQGLWTIQQVSKPGELVQKILDVS
                     PDVVFIDLGTTGLNIFDELDQVATLQHQPPYVVYSREDCFLFHRFAMRHSVVAYFPIP
                     CDFSRMRTRLESILDPVITTKEDICLSGEAGKALLGESLAMRSLRKRIQLVSSRSEPV
                     LITGETGSGKELVARCIHENSPFRSGPYIARNISCISSGLADAYFFGNVKGSFTGAED
                     QKGI"
BASE COUNT          646 a          638 c          667 g          710 t
ORIGIN      
        1 tatacgccta gcgacgcgaa tatcgacaag cttggcattc ctccggaccg cgaggtgttg
       61 cttcctgaat taaccgaggc cgaggaaaag gccctaagcg aattattgca aacaacccgt
      121 ctaaacgatc ttgtggcggg taaaactgaa ttacgagaca cagaggccga gatacttgct
      181 cgagaattgg cgaaaaccta tccggtgagt ttacgcgtgc ttaaaaaact ggtgtatcag
      241 gagttttatc gcacacactt gagcccgctc tatgatcttg aatttgacct tcagctaaaa
      301 gccgcggtag acatcctgct aaaagaaaac attcgcgagc ttcttaggtc taccaaaacg
      361 gttcacgaac tgcagctttc gggtgaccaa gcggtttcga ccgcctcttc cgggtcatga
      421 ggcagttact cgtcgacagt cttcctgctg tcgacggcac tcttaccctg cgcggctcac
      481 aatatcgcta tgttcgtcgc gtcctgagaa agggcgcggg ggactcgctt gagtgccgtt
      541 ttgtcgatgg atccttggcg ttctgtacag ttcttgaagt ggacgattcc cgcaagagca
      601 taaccctgtc tgtatcagag gggggtatcc ctcaggtaga gccgggctat tcccaatcgg
      661 catcttcact cgccctggat aattttcccc ggcttgtgct gcttcaatgg attcttaagg
      721 gccaaaagat ggacctggtt atccgacaag ctaccgaggc aggcgttatg gacataattc
      781 ctgttgtggg cgatcgatcg attcccgaac gcgaatccag ttccagaagt gaacgatggt
      841 tgcgaattgt tcgcgaggcc agacaacaaa gcggctctgc ggtgcccacg actattcatc
      901 cagccacaat gtcctcagcc attgtcaatg tatccaatca gttgctttcc ggggaaagcc
      961 cgacccggct cgtctttacc gaagcccctc ttgcgttaaa gtcactccat cagtatcttg
     1021 attcagggcc ggcaacagtc gtcctggcca taggtccgga gggagggatg agtcctgccg
     1081 aactgacagc cttggggaca gcgggtttta actgcgtcca tttcaagaca aatattctaa
     1141 gggcagagac ctgcgcttta tatgccgtcg cggcaacgct gagcgcccta acggagagag
     1201 atacgtggca gttgaaagaa tacatctgtt aaaagttccc ttggacattg ttcatgaaga
     1261 agatatggaa gaaatggttc ttgcgctcct ttctcgggat ggtccccagc atattatcat
     1321 gctctctctt tgggatttta tgcgcgcacg tcatcggggc gaattccaat ctcttgtaag
     1381 caaggccgct ttggttatac ccacttcccg cgccttggtg cgtggagcca gattccttaa
     1441 aaagactgcg cccgtgcgct atcagtcctt ttcctttatc atcaatgtgc tcggtatttt
     1501 ggagcgatac tataaaacgc tctatctttt gggtggcaat aatcggagct tgatgcaggc
     1561 ggaaaaaaac gtgcgcagca cctttccgca tcttgggata gtgggccgct ttgccgggca
     1621 ttatcaccgc agtatggagc gaaagatcca gactgcaatc gttaaggccc atccatctct
     1681 ggttatcatg ggtaacggaa tacccggcgg acagcgatgg atatatcgaa acaagagcaa
     1741 gctgcacagt ggcatctttt tttgggatac cgatgtaatt gatattttct ccgaacgtaa
     1801 acgacgggtt tcagataaaa cttttgagcg aggacatgaa tacctgggtc aaattgtaaa
     1861 aaatccattt agaattttgc ggttttttca atacctgtgg tacaatctcg ttttacttta
     1921 ctaccggttg ttcagggttt atagctgatc accatgtagt tcaacgaatc cgggagcaat
     1981 caaaaggatt caaaattgaa aaacatactc tatcttacct cgtttacaaa cgacgtggcc
     2041 catctctctt acgaagccca aggattatgg actattcagc aagtatccaa acccggagag
     2101 ttagttcaaa aaattcttga tgtatctcct gatgtggtgt ttattgatct agggacaacc
     2161 ggactaaata tttttgatga gctggaccaa gtcgcgacgc ttcagcatca gcctccatat
     2221 gtcgtgtatt caagggagga ctgttttttg tttcatcgtt tcgcgatgcg ccatagtgtg
     2281 gtcgcgtact tccctattcc ctgtgatttt tcccgtatgc gcacacgcct tgagtcaatt
     2341 ctggatccgg taattaccac caaagaggat atctgtttgt ccggtgaagc cggaaaggct
     2401 cttttgggcg aaagccttgc catgcgctca ttgcgtaagc gaatacagtt ggtaagttca
     2461 cgctctgagc cggtgttgat taccggcgaa acgggtagtg gcaaggaact ggttgccagg
     2521 tgtattcatg aaaactcacc gttcaggagc gggccgtata tagcgcgcaa tattagttgt
     2581 atttcttccg gccttgccga cgcttacttt tttggaaatg taaagggttc gtttaccggg
     2641 gcagaggatc aaaagggtat c
//
LOCUS       DAOQXQ010000187         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42354_length_2650_cov_2.768015, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000187 DAOQXQ010000000
VERSION     DAOQXQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42354_length_2650_cov_2.768015"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKH81_03960"
     CDS             <1..964
                     /locus_tag="PKH81_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97224.1"
                     /translation="LKVSDWNGRITEFPQDSTFDRYNEVIWLNNEEILIQHDTTMLMW
                     NLAQNKITTPLKNIKIDNVFKFSTSGKALVNADGKTGIVDIATQRIEWLPDFGEGFYE
                     WSPDGKSLYFRKRNDDNTGDFTIYDRLAKRLRHIKTYSRRFCNVVFLRKVTAYLFFED
                     DNPDSSTGGYPIWILSLITGEWVNLSKQSGYSWYGVMACPHLYYSEGQEWVLDSEVLG
                     SCSGKAREGLDFKKVSRSRVVNGLLHIRIEENLYETTWLDSVALRLGEKRILPEGCPA
                     ELSATDGQYHVLQMNDFIELTFPVPAEDEGALVLEFGGYYEAGP"
     gene            complement(1045..1464)
                     /locus_tag="PKH81_03965"
     CDS             complement(1045..1464)
                     /locus_tag="PKH81_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ97225.1"
                     /translation="MNYLLDTCVLSETIKPKPDPGLVTWLTAQQESTLFVSTLTLGEL
                     RKGIERLPSGKKKHDLLLWLTRLCSAYRARFLEFDSSCAMVWGEITALAENKGHPLPV
                     IDSMLAATALSNNCILVTRNVNDYSKTNVRLVSPWEE"
     gene            complement(1461..1706)
                     /locus_tag="PKH81_03970"
     CDS             complement(1461..1706)
                     /locus_tag="PKH81_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNQ97226.1"
                     /translation="MTQKWQLQDAKARFSELFSEVLSSGAQYVSRRGKETIVLIPEDE
                     YRKLKGEESSFISFLFSSPKATLDLARSQDSGRTVDL"
     gene            complement(1767..>2650)
                     /locus_tag="PKH81_03975"
     CDS             complement(1767..>2650)
                     /locus_tag="PKH81_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNQ97227.1"
                     /translation="TFLSGPLLLQYLQVMTKYIVAAIVLFSSFLPHLSAEESRDTSPS
                     PLVGFWDNTIESHTGVNSLWHIGSLAATGIMFMTPVDQKVMEADKNYFGDTVNIGALI
                     VGTWWHVLPAAIVYWTTDNTEHKYASSAVVQAVGISFLVTTTEKFIFGRPFIDKGDGR
                     KPGFLPVRTSRDARDFYPFQSPSGLWPSGHTATAFAAASALAAFYQDDESFIPVAILA
                     YSASALMGYAMIDGNIHWASDVVAGVLIGHAIGWTVGSDFKNRYDGTQKAARLTQPSF
                     IPLIAHKTYGVMVTIGY"
BASE COUNT          663 a          569 c          719 g          699 t
ORIGIN      
        1 tcttaaggta tccgactgga acggaaggat tactgagttt ccgcaggata gtacctttga
       61 ccgctataat gaagtgatct ggttgaacaa tgaagaaatc ctgatacagc atgatactac
      121 catgttgatg tggaatcttg cacagaacaa gattacaact cctcttaaaa atataaaaat
      181 tgacaatgtg ttcaaatttt caaccagtgg taaggctctg gtaaatgctg acggaaagac
      241 cgggattgtg gatattgcca ctcaacgaat tgaatggtta cccgacttcg gtgagggctt
      301 ctatgagtgg tctcccgacg gaaaatctct ctactttaga aaaagaaatg atgataatac
      361 cggagatttt accatttatg atcgtctggc aaaacgcttg cggcatatca aaacgtatag
      421 tagaagattc tgtaatgtag tcttcctccg taaggtgact gcctatctgt tttttgagga
      481 tgataacccg gactcctcta ccgggggcta tccgatatgg atactgagtc tgattaccgg
      541 agaatgggta aacctttcca agcaaagcgg gtattcctgg tacggggtta tggcttgccc
      601 gcatctctat tattccgaag ggcaggaatg ggttctggat agcgaggttc tgggatcttg
      661 ttctggaaaa gcaagggagg gtcttgattt taaaaaggtt tcacggtcac gtgtggtgaa
      721 cggcctttta catatcagga tcgaagaaaa cctttacgaa acaacctggc ttgattcagt
      781 cgccttacgc cttggtgaga agcggattct tccagaagga tgtcctgcag aattaagcgc
      841 caccgacgga cagtatcacg ttctgcagat gaatgatttc attgagctta cctttccggt
      901 tcctgccgaa gatgagggag cccttgtact tgaatttggc ggctactatg aggcgggacc
      961 ctagatcgcc ccttgaggga ctttcgtctg agggggctcc gaacgatgaa tgtcgccagc
     1021 cgttacgttt tatgcctcaa ttttttactc ttcccaagga ctgaccagtc gtacgtttgt
     1081 tttggaataa tcgttgacgt ttcgtgttac cagtatacag ttattactaa gagcagttgc
     1141 ggcgagcatg gagtcaatga cggggagggg atgccctttg ttttctgcca gggctgttat
     1201 ctcgccccag accattgcgc agcttgagtc aaactcaagg aatcgggcgc ggtacgccga
     1261 gcagagacgg gttagccaca gaagcaagtc gtgctttttt ttacctgaag gaagtctctc
     1321 aataccttta cgaagttcgc cgagagtgag cgtacttaca aagagagttg actcctgttg
     1381 tgcggtgagc caggtaacca ggccgggatc cggttttggt ttgatggttt ccgagaggac
     1441 gcaggtgtca agaaggtagt tcataggtct accgttcgcc cgctgtcttg cgacctggca
     1501 agatcaagcg ttgccttggg agaagaaaac aggaaagaga taaaagatga ctcttctcct
     1561 ttgagttttc ggtattcgtc ttccggaata agaacaatgg tttccttccc acgccggcta
     1621 acgtattggg ctccggagct gagaacttcg gaaaagagtt cgctaaagcg tgcttttgcg
     1681 tcctgtaatt gccatttctg tgtcatgtct atacctctca gtataaagta taaacagacc
     1741 agactgacta gtcaacagga ggtttcttag tatccgatgg ttaccatcac gccgtaggtt
     1801 ttatgagcta taaggggtat aaacgatggc tgggtaagac gtgcggcttt ttgtgtgccg
     1861 tcataacggt ttttgaaatc gcttccgact gtccagccga ttgcgtggcc gataaggaca
     1921 ccggctacca cgtctgatgc ccagtggatg ttgccgtcta tcatggcgta acccataagg
     1981 gcggatgcgc tgtaggcgag tatggctacc ggtataaagg attcgtcgtc ttgataaaac
     2041 gcggccaggg cagaggctgc ggcaaaggcg gttgcggtgt gaccggaggg ccataagccg
     2101 cttgggcttt ggaatggata aaagtcgcgg gcatcgcggc tggtgcgcac cgggagaaag
     2161 ccgggctttc ggccatctcc tttgtcgata aagggtcggc caaagataaa cttttctgtc
     2221 gtggttacca ggaaagagat tccaactgcc tgcacgacag cagaggatgc gtatttgtgc
     2281 tctgtgttat cggtcgtcca gtagactatg gctgcgggga gtacatgcca ccaggtacca
     2341 acgatcaggg ctccgatatt aacggtgtct ccaaaatagt ttttatccgc ttccattact
     2401 ttttggtcaa caggggtcat aaacataatt cctgttgccg cgagagagcc gatatgccaa
     2461 agagaattaa ctccggtgtg gctctctatg gtattgtccc aaaatccgac aagcggagag
     2521 ggggaagtgt cccgggattc ctcggcactc aggtggggaa ggaagcttga aaacaatact
     2581 atggcggcaa ctatgtattt tgtcataacc tgtagatact gtagcaaaag tggcccggag
     2641 aggaaagtcc
//
LOCUS       DAOQXQ010000188         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42554_length_2642_cov_2.393119, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000188 DAOQXQ010000000
VERSION     DAOQXQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42554_length_2642_cov_2.393119"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKH81_03980"
     CDS             <1..221
                     /locus_tag="PKH81_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ97228.1"
                     /translation="MKDAAEGDLKGVLKYTEDAVVSTDFRGESCTSIFDATAGISLDA
                     NFVKVVSWYDNEWGYSNKVCEMAKVVAK"
     gene            361..1656
                     /locus_tag="PKH81_03985"
     CDS             361..1656
                     /locus_tag="PKH81_03985"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNQ97229.1"
                     /translation="MSNIVVGVIGFGTVGKGVVKILLHDRAILEQRSGCSIELKKIVD
                     LDITTDRGIPLPSGILSTDMADVMNDPAINVVVEVMGGENAAKKCIESALSAGKNVVT
                     SNKEVMAKHGLYFTRLAKEHQCTILYEAAVGGGIPVLHALRNSLSANTVETIYGIVNG
                     TTNFILSKMTEDGSDFDETLKEAQALGYAEANPKNDVEGYDATYKLAILASLAFRTHV
                     NYNDIYREGITAITAADIAAARQFGYTIKILAIGVNHPDSLELRVHPVMIDSSHPLAS
                     VRDAFNAIFIKGSSLGESMLYGRGAGELPTASAVVGDIMNLAFTKTPGFARDTDFALN
                     ERAVTSINECVTSFFIRCIVDDAPGVLASIANVFGKNEVSIRALEQIDGRDKSASITI
                     ITHQVKEERMRAALNEIEGLPCVQKVASVIRVGLGEERI"
     gene            1653..2519
                     /locus_tag="PKH81_03990"
     CDS             1653..2519
                     /locus_tag="PKH81_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ97230.1"
                     /translation="MTYDTIIIGKGPAGISAALYLVRSGYTILVLGSSVGALERAETI
                     ENYYGFPQALSGSDLASRGIAQAERLGVEIRSEEVVHLGMEELWSVKTDAATYTAKTV
                     LLATGKSRSTLKVPGFEEYRGKGISFCVTCDGFLYRGKTLALLGAGEYAANEWAELGV
                     FTQDRMVFTNGAEVTGTFPADTRFITQKITAFAGADTVTGITTEDGTTHPVQGVFVAL
                     GTAGATSFAATLGIELTGTDIVVDSEYMTNIPGIFAAGDCTGGFLQIAKAVSDGALAA
                     KGIHVYLKKIFT"
     gene            2568..>2642
                     /gene="pgk"
                     /locus_tag="PKH81_03995"
     CDS             2568..>2642
                     /gene="pgk"
                     /locus_tag="PKH81_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNQ97231.1"
                     /translation="MIKTIKDVALTGKRVIMRVDFNVPM"
BASE COUNT          648 a          635 c          680 g          679 t
ORIGIN      
        1 ctatgaagga cgctgcagaa ggcgacctta agggcgttct taagtatacc gaggatgctg
       61 ttgtttccac cgacttccgc ggtgaatctt gcacctcaat cttcgatgct actgccggta
      121 tctcccttga tgctaacttc gtcaaggttg tttcctggta cgacaacgaa tggggttatt
      181 ccaacaaggt atgcgaaatg gctaaggtcg tggcgaagta aaaaacttcg cttgaatggc
      241 attatgcaag aggggtcccg caagggaccc ttttttttat cccgtaatcc tgctttcttg
      301 accataccgc ccacacaagt tattctggag acaaatactg tgttcgccaa agaggtagtt
      361 atgtccaaca ttgttgtggg agtgatcggg tttggaacgg taggtaaggg tgtcgtcaag
      421 attttattac acgatcgcgc catccttgaa cagcgttccg gatgttcgat agaactaaaa
      481 aagatagtag acctggatat caccaccgat aggggcatcc cccttccttc gggaatcctt
      541 tccacagaca tggctgacgt aatgaacgat ccggcaatca acgtggttgt ggaagtgatg
      601 ggcggagaaa acgccgccaa gaaatgtatc gaaagcgcct tgagcgccgg aaagaatgta
      661 gtcacttcaa acaaggaggt catggcaaag catggccttt attttacccg acttgcgaag
      721 gaacaccagt gcactatcct ctacgaggcg gcagttggcg gcgggattcc agtccttcac
      781 gcacttcgca attctctttc cgctaatacc gtggaaacta tctatggtat tgttaacgga
      841 actaccaatt ttatcctttc caaaatgaca gaggatggat ctgattttga tgagactctc
      901 aaggaagccc aagccctagg ctatgccgag gctaatccca aaaatgatgt tgaagggtat
      961 gacgcgacgt ataaacttgc catccttgcc agtctcgctt ttcgcactca cgtcaattat
     1021 aacgatattt atcgggaagg tattactgcc attaccgccg cagacatagc cgcagctcgt
     1081 caatttggct ataccataaa aatcttggcc attggtgtaa accaccctga ttctcttgag
     1141 cttcgcgttc acccggtgat gatagattcc tcgcatccct tggcatcggt tcgggatgca
     1201 tttaacgcga tctttattaa gggttcaagt ctgggcgaat caatgctgta tggacgggga
     1261 gcgggagagc ttcctaccgc ttctgcagtc gtaggggata tcatgaacct tgcctttaca
     1321 aagacacccg gttttgcccg cgataccgat tttgccctta atgagcgagc ggtaacctcc
     1381 attaacgaat gcgttaccag tttctttata cgctgtatcg tcgatgacgc cccaggcgtc
     1441 ctggcgagta tcgcgaacgt gtttggcaaa aacgaggtaa gtattcgcgc tctggagcaa
     1501 attgacggac gcgataaaag cgcctccatc actattatta cccaccaggt aaaagaagag
     1561 cgcatgcgag cagcccttaa tgaaattgaa gggctgccct gcgttcaaaa ggttgcttcg
     1621 gtaatccgtg taggccttgg tgaagagcga atatgacata cgatacaatc atcatcggta
     1681 aggggcctgc gggtatctct gcggctttgt atctagtgcg ctcgggctat accattcttg
     1741 ttctgggctc gtctgttggc gctcttgagc gtgccgaaac gatcgaaaac tactatggct
     1801 ttcctcaagc gctttccggc tctgaccttg cttcacgggg aatcgctcag gcagagcgac
     1861 ttggtgtgga gattcgaagt gaggaagtgg ttcaccttgg catggaagaa ctctggtcgg
     1921 taaaaacgga tgctgccacg tatacggcaa aaacggtact actggcgacc ggtaaatccc
     1981 gctcaacgct caaggtaccc ggttttgagg agtatcgcgg caagggcatc agtttttgcg
     2041 ttacctgcga cgggttcctc tatcgaggca agacccttgc acttcttggc gcaggagagt
     2101 atgccgcaaa cgagtgggcg gaactgggtg tctttaccca agaccgcatg gtgtttacta
     2161 acggagccga agtcactggc acctttcccg ctgatacccg ttttatcact caaaagatta
     2221 ccgccttcgc cggggccgat accgttacgg gcattaccac agaagacgga actactcatc
     2281 cggttcaggg tgtttttgtt gccctgggaa ccgccggcgc aaccagtttt gccgcaaccc
     2341 tggggattga gcttacgggc accgacatcg tcgttgacag tgagtatatg acgaacattc
     2401 caggcatctt tgccgcaggc gattgtaccg gtggtttttt acagattgcc aaggcagttt
     2461 cggatggtgc cctcgcggcc aagggcatac atgtatatct taagaagata tttacataac
     2521 aacggttaat tggtatattt tgcacgattt actaggagga atagtatatg atcaagacca
     2581 ttaaggatgt tgcgcttacc ggaaagcgcg ttatcatgcg cgtggatttc aatgtaccga
     2641 tg
//
LOCUS       DAOQXQ010000189         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42589_length_2640_cov_3.023211, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000189 DAOQXQ010000000
VERSION     DAOQXQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42589_length_2640_cov_3.023211"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..894)
                     /locus_tag="PKH81_04000"
     CDS             complement(340..894)
                     /locus_tag="PKH81_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ97232.1"
                     /translation="MLESFITSKTRVKLLLKFFINPDTTAYLRGLADEFGESTNAVRV
                     ELNRLSRAGILESRAEGRTILYKANKKHPLFPEVQRIVAKTVGLDRLVEQVVSRLGSV
                     ELAFVTGDYARGVDSGLIDLVLVGEVDEIYLAELAKKAEKLIDRKIRTLVLARDEYQK
                     LEEKFEKEKAILVWDNPKGYGLSM"
     gene            complement(1241..1567)
                     /locus_tag="PKH81_04005"
     CDS             complement(1241..1567)
                     /locus_tag="PKH81_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNQ97233.1"
                     /translation="MGNTKGGSLFENAAFTTLRAWSSLKPGTALFWYLDRNNMEADFI
                     LQGRDGNIGFEVKKTTSVNLSHAKTLIEARREGLIKEGYVLFDGEGKQQLLEGVFALG
                     LRSLVP"
     gene            2120..>2640
                     /locus_tag="PKH81_04010"
     CDS             2120..>2640
                     /locus_tag="PKH81_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97234.1"
                     /translation="MRDRVNDLPSLADSPSDRVRPWAPGDEFRQKVAQTVKDWENGRL
                     PDLDKALKKLSRDHNESPIDEFYGLSPKQMHELLILGWWNDEKVINLNDDLPADMLEN
                     APLLHNCRVLLEIVAGSGGVKATARGNLKRDTVQQVYDKAIFKGFDLYDVYWQPGVIN
                     EEDAPAVHMARIV"
BASE COUNT          616 a          583 c          833 g          608 t
ORIGIN      
        1 gatgtcgggc tgtcgtgccc tgacatatat aagagtactt cggaagggtt agatatacaa
       61 atagtaaacc taagggcatt ggggtcagac cttgaatttg agcatttgtg tacaaaaagg
      121 caggaggggc gaaaggaggg agccgtgaat cgttaatcgt taatcaggaa tcggatgggc
      181 gggaggccgg aaagcaggag gcggaggaag gtcaggagcg tgagaggcta gacacgaaac
      241 ggaagagcgg gaagcaagga gacagacgtc gggcgacagg gaaggcttaa aacgtaaatc
      301 ggggtttggt aatcgggtgt gatttccccg cttacccttt tacattgaca gtccataacc
      361 cttggggtta tcccacacca ggatggcctt ttctttttcg aacttctcct ccagtttctg
      421 atactcgtcg cgggcgagga caagggtgcg gatcttgcgg tcgataagtt tctcggcctt
      481 tttggccaat tcggcgaggt agatctcgtc gacctccccg accaggacga ggtcgatgag
      541 gcccgagtca acgccgcggg cgtagtcgcc ggtgacgaag gccagctcca cgctgcccaa
      601 gcgggatacg acctgctcga caagcctgtc gaggcccacg gtcttggcga cgatgcgctg
      661 gacttcgggg aaaagggggt gctttttgtt ggccttgtag aggatggtgc ggccttcggc
      721 cctggactcg aggatgccgg ccctggagag acggttcagt tccactcgca cggcgttggt
      781 ggattcgccg aattcgtcgg ccagccctcg caggtaggcg gtggtgtcgg gattgatgaa
      841 gaacttgagg agcagtttga cgcgcgtttt cgaggtgatg aagctttcga gcatggatcc
      901 ctccagcggg gctaaaagtt tagcgactgg ctttaggagc cggcttcaag gcacgaacaa
      961 tgggacctca aggcaaggtg acgaagcctt gaacttctgg tagtaacgct gcttttcgct
     1021 cggcaatgtt tacgagtaat tttattgctc tttgggagga aaggcaaggg ccgtgaatcg
     1081 tcaatcggga atcggagggc aggagacgga aagacaagga ggtaagagac ggcagcgggc
     1141 aacaggtagt gcaaaagcaa aaggacaaga gcttaaagcg aaggtggaat ccgccaactg
     1201 gggatcgggg acaaggtttt cacgttggac tatcaaggtt ttaaggcacc agggacctga
     1261 gcccgagggc gaatacgcct tccaatagtt gctgtttacc ctctccgtcg aagaggacat
     1321 atccctcttt tatcaggcct tcgcgcctcg cttctataag tgttttcgcg tgggacaggt
     1381 tgacggaggt ggttttcttg acttcgaaac cgatgttgcc gtcgcggccc tggaggatga
     1441 agtcggcttc catgttgtta cggtcgagat accagaaaag ggctgtgccc ggttttaggg
     1501 aactccaggc gcgaagggtc gtgaaagctg cgttttcgaa gaggctcccg cctttcgtgt
     1561 tccccaggta ggaggcgaac ccggggtcag ggcgagaaag tcgttaatag tgaatcgaaa
     1621 tgggcaaggc caaacccttt tcaccactaa aacgggacga ctggctgtag gctggatctt
     1681 cgatctcccc acggttatgc tgctcgctcg aaatgacagc caggagcgtt cgttgctttt
     1741 ttgtgccttt ttgaaaaatt gtccaggttg ttgcgactgg ttcccgaagc gctatagtga
     1801 aacctgtaat ccatggcgtt aatcatgggt tggggaatca gccttcatgt ttggggtgat
     1861 agatatggga ttttgggatt caggggaaac gcgtttgccg ctttcggaag tagaaagaag
     1921 ggtgcttctg ctttcgtcgc cgttggcaag gagagctttt ggttcgtttt tgagaggtga
     1981 ccttgaaagg gaaggtgata tttggatcgc gttgcttaat tttgacgaat tgctcctgat
     2041 gggggaatgt atcaacagag acgtatgcct tttggcggcc gggaaggacc ggacaattct
     2101 tgaggggatc ctttcgaagg tgcgagaccg cgttaatgat ctgccttccc tcgcagattc
     2161 tccttcggac agggttcgcc cctgggcgcc gggcgatgaa ttcaggcaaa aggtagccca
     2221 aacagtaaaa gactgggaaa atgggcgctt accggatttg gacaaggcgc tgaaaaaact
     2281 ctcccgcgat cacaacgagt ctccaattga cgaattctac gggctttcac cgaaacaaat
     2341 gcacgagctt ttgatcctgg gctggtggaa tgacgaaaag gtaataaacc tgaacgacga
     2401 cctgcccgcc gatatgcttg aaaacgcccc gctgcttcat aactgccgag ttctgctgga
     2461 gattgtagcg ggatcaggcg gggtaaaggc tacggcgcgg gggaatctga aaagagatac
     2521 ggtgcaacag gtttacgata aggccatttt caagggtttc gatttgtacg atgtgtactg
     2581 gcagccaggc gtgattaacg aggaagatgc tccggccgtg catatggcca ggatagtatg
//
LOCUS       DAOQXQ010000190         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42673_length_2636_cov_3.176676, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000190 DAOQXQ010000000
VERSION     DAOQXQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42673_length_2636_cov_3.176676"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PKH81_04015"
     CDS             complement(<1..968)
                     /locus_tag="PKH81_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ97235.1"
                     /translation="MSKKIIFLLAVLVVLGPCVLFAGGQKDSGMIKVGIINNPPSESG
                     YRAANVADFEKVFTKENGYQVSTFYSLKNDEQLNAASQFITNGVDYILLSAAATDGWE
                     PILTRAKEAGIKVFLFDRMLNAKDSLYEAAVISDMAQQGKTAVAWLKAQKLPVYNVIH
                     IQGAMGSDAQIGRTAALNAEFSAGNMKKVVQQTATWDEAEAKKIVESVINSKQAFNVI
                     YAENDGMAKGAVAALDAAGITHGVDKAVVVMGFDCNKWALRELLAKNWNYDGQCSPFQ
                     AKVIDGMIKTLQSGGTIANKKVISEEKGFDARTITQKDIDMYGLGN"
     gene            complement(1142..2548)
                     /locus_tag="PKH81_04020"
     CDS             complement(1142..2548)
                     /locus_tag="PKH81_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNQ97236.1"
                     /translation="MYGITFDTRSRLSQYRQLVDQLREKITNGTLTGGTRLASTRELS
                     ERLGIARGIVLEAVDQLKMEGYLTTVHGSGTFIREGLVWDSLSRSGPISSIEARHTHQ
                     DRPSPVLSFAPGMPDMTLFPRRAWLSCYHDAVEYASLDDLGYNRPSGDWNLRVAISSH
                     LYEIKGITAGPERIIVTAGASQAIAMLASLFDSPRIVMEDPQAPFIRRIFDGLGCDIV
                     YAPVDEEGIREDLVTCDKADLIYVTPTHQFPVGGSLSAERRIALLAKARSLGAWIIED
                     DFDSEFRYDGNPVTPLQVMANERVVYVGTFSKTVSPALRIGFMVVPPGIQTRIKTLKR
                     RWDFWNEGFQQKAMALFIEKGYLARHLARAHRSYRRKNAFLKECITQRLSPRWRITGS
                     TTGMHLVIRRCAQDGKTVDVAPFKAALAKQGLEVESIHDYCQKYYAFPDALIIAFGNR
                     SEAELTRLVCAIDDIDKS"
BASE COUNT          695 a          637 c          602 g          702 t
ORIGIN      
        1 tagttgccaa gaccatacat atcgatgtct ttctgggtaa tcgtacgtgc gtcgaatccc
       61 ttttcttcag agataacttt cttgttggcg attgttccac cagactgcag tgtcttgatc
      121 attccatcaa taacctttgc ctggaaggga ctgcactgtc catcatagtt ccagtttttg
      181 gcaagcaact ctctgagtgc ccacttgttg cagtcaaagc ccattactac tacagctttg
      241 tccacgccat gagtaattcc ggcagcgtca agagcggcaa cggcaccctt ggccataccg
      301 tcattctcgg catagattac gttaaaggct tgcttggagt tgataacaga ttcaacaatc
      361 ttcttggctt ctgcttcgtc ccaggtcgcg gtttgctgaa cgactttttt catgttgcca
      421 gcggagaact cggcattaag agctgcagta cgtccaatct gagcatcgct tcccattgca
      481 ccctgaatgt gaattacatt atataccggg agcttttgag ccttaagcca agcaacagcg
      541 gtctttccct gctgagccat atcggatata acagcagctt cataaaggct gtctttggca
      601 ttaagcatgc ggtcaaaaag gaatactttg ataccagctt ctttagcgcg agttaaaatc
      661 ggctcccatc catctgtggc agcggctgac agaagaatat aatctacgcc attggtaata
      721 aactggcttg cggcgttaag ctgctcgtcg ttcttaaggc tgtagaaagt agaaacctgg
      781 tagccattct ctttggtaaa tactttttca aagtcggcta cgtttgctgc gcggtatcct
      841 gattcagaag gcgggttatt gataatacca accttaatca ttccagaatc tttttgtccg
      901 cccgcgaata aaacgcacgg tcccaataca accagcacag caagcaagaa aataatcttt
      961 ttgctcataa aaatcctcct tatgaagcaa atgaaaatag aattacttgt agtgtggggc
     1021 cacattgggt aaaagtacat gaagtatctt atcgactttg tttgacaaat ttacgtattt
     1081 tttttattca tgattctgat ttgttataaa aggggatttt ttactgaaaa agtgtgtttt
     1141 tttacgattt atcgatgtca tcaatcgcac aaaccagccg ggttaattcg gcttcactgc
     1201 ggtttccaaa ggcgataata agagcgtcag gaaaagcata atacttctgg caataatcat
     1261 gtattgattc aacctcaagt ccttgcttgg caagagctgc tttaaaagga gccacatcaa
     1321 ctgtcttgcc atcttgggca caacgcctga ttaccagatg cattccggtg gtagatcccg
     1381 taattcgcca gcggggagaa aggcgttgag taatacactc tttcaggaaa gcgtttttcc
     1441 ggcgatacga gcgatgtgct cgtgcaaggt gtcgagcaag gtaacccttt tcaataaaga
     1501 gggccattgc tttttgctgg aaaccctcgt tccaaaagtc ccaccttcgc ttgagtgttt
     1561 taatgcgggt ctggattccc ggaggtacaa ccataaagcc aatacggagt gccgggctta
     1621 ccgttttact aaaggttccc acatacacaa ctcgctcgtt agccattacc tgaagcggtg
     1681 ttaccggatt gccatcatag cgaaactcgc tgtcaaaatc gtcctcaata atccacgcgc
     1741 caagactgcg agccttggca agaagggcaa tccttcgttc ggcactcagg gagccgccaa
     1801 ccgggaactg atgagtcgga gtaacataga tcaggtctgc cttgtcgcaa gtaactaaat
     1861 cttcgcgaat accttcttca tcaacgggtg catacacaat atcgcagcct aagccgtcaa
     1921 agattcgtct gataaacgga gcctgtggat cttccattac aattcgaggg gaatcaaaaa
     1981 gcgaagcgag cattgcaatg gcttgggaag cgcccgcagt tacgatgatc cgttccggac
     2041 ccgccgtaat tcctttgatc tcgtatagat ggctcgaaat tgcgacgcgc aagttccaat
     2101 ctcccgaagg tcggttgtat ccaagatcat caaggctcgc gtattccacc gcatcatgat
     2161 aacaggaaag ccaggcccga cgcgggaaaa gcgtcatatc cggcatcccg ggggcaaagg
     2221 acagtacagg ggatggcctg tcctgatgag tgtgtcgtgc ctcgattgag gagattggcc
     2281 cggatcggga gagtgagtcc cacacaagac cctcgcgtat aaaggtcccc gaaccatgaa
     2341 cagtagtcag gtatccttcc attttaagtt ggtctaccgc ctcaaggaca atccctcgcg
     2401 caataccaag ccgctcggaa agctctctgg ttgaggcaag ccgagtacca ccggtaaggg
     2461 ttccgtttgt aattttctct cttaactgat caacgagctg acggtactga cttaaacggg
     2521 aacgtgtatc gaaggtaata ccatacatag tgacatgata tccttgttta ttccgtagct
     2581 caagtggttc acaaaacaaa gtgtaaactg gcgctactta aaccactttc ctggac
//
LOCUS       DAOQXQ010000191         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42773_length_2632_cov_3.098176, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000191 DAOQXQ010000000
VERSION     DAOQXQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42773_length_2632_cov_3.098176"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..742)
                     /locus_tag="PKH81_04025"
     CDS             complement(14..742)
                     /locus_tag="PKH81_04025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ97237.1"
                     /translation="MKYLVTGSAGFIGFHVCRTLLRAGHEVVGFDSVNNYYRQDLKEE
                     RLTILEAEGANAPGRFRFFRGLLEDSDLLATAFACGPFSAVIHLAAQAGVRYSTTHPQ
                     AYLSSNLEGFLSVLELCRTFSVPHLAFASSSSVYGLNRTVPFSENHSTDHTVSLYAAT
                     KKANEVMAHTYSHLYGIPATGMRFFTVYGPYGRPDMAYFKFADTIMRKETIDIYNNGD
                     LLRDFTYIDDVVRAIIMIASANKK"
     gene            complement(739..1281)
                     /locus_tag="PKH81_04030"
     CDS             complement(739..1281)
                     /locus_tag="PKH81_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNQ97238.1"
                     /translation="MEQDSEFLLLETIHDLTQSQADISQRDLSRAINMSLGMTNALLK
                     RLSQKGFIIIQKISPRNVSYVLTPDGINELAGRTYRYLKRTIKKVVDYKEVIVTVVKE
                     AKKRGFSRIGLLGQSDIDFIIEWAASSVELEYCTFASTDDIPADSFVFVSENLAQPPV
                     PVTAQMPAIHIAEILSGALT"
     gene            1447..>2632
                     /locus_tag="PKH81_04035"
     CDS             1447..>2632
                     /locus_tag="PKH81_04035"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNQ97239.1"
                     /translation="MPVAPYIKDLMTSSSFIRRMFEEGIQMKTQFGADKVYDFSIGNP
                     DLEPPAKVKTVLQRLAASTEPSLHGYMPNAGYPQTRAAMAQKVSIEQGITVDFSRIVM
                     GVGAAGAMNSVLKAILSPSDEVLVSAPFFVEYTQYVRNHGGTLITVPAKEDFSLDITA
                     IAGALTSKTCAIIINSPNNPTGKIYTEDEIIALARTLEQHREKTGKTVLIIADEPYRE
                     ILYDNKTVAPLFPHWKDSVIVTSFAKNLSLPGERIGYTAINPSCPDAQELTDAIIYAT
                     RVLGYVNAPATFQRVVAECWNEPVDYSLYTQRRNILTSILDKAGISYAAPEGAFYLFC
                     KVPQCLSQPDRSGDDAAFCDHLKKYRILGVPGRGFGKSGWFRLAYCVSLASVQNSEAA
                     FIQ"
BASE COUNT          773 a          600 c          564 g          695 t
ORIGIN      
        1 ggcctgatat ggcctatttt ttattcgcag atgcaatcat gatgatagct ctcacaacat
       61 cgtctatgta ggtaaagtct ctcaataaat caccgttatt gtatatatcg atcgtttctt
      121 ttctcataat cgtatctgcg aatttaaaat aggccatatc aggcctgcca tagggcccgt
      181 atacggtaaa aaaacgcatt cctgtagccg gtattccgta caaatgacta taagtatgag
      241 ccatcacctc atttgccttt tttgttgctg catacaagct aaccgtatga tccgtcgaat
      301 gattttcgga gaaaggtacg gttcgattca atccatacac tgacgaactg gaagcaaagg
      361 ccaaatgtgg aaccgaaaaa gtgcgacaca attctaaaac agagagaaaa ccttcgaggt
      421 tactcgaaag ataggcttgc ggatgagtgg tactgtagcg aacacctgcc tgggctgcaa
      481 gatgaataac agccgaaaaa gggccacatg cgaaggctgt agcaagaaga tctgaatcct
      541 caagaaggcc tctaaaaaat cggaaccggc caggagcatt agcaccttct gcttccaaaa
      601 tggtaaggcg ctcttctttt agatcctggc gataataatt gtttaccgag tcaaaaccga
      661 caacctcatg accggcgcgt aaaagagtgc gacaaacatg aaaaccgata aatcctgcgg
      721 atccggtaac aagatacttc atgtaagtgc ccctgaaaga atttccgcta tatgaatagc
      781 aggcatttga gcggttacgg gaaccggcgg ttgcgctagg ttttctgaga caaacacaaa
      841 ggaatctgct ggaatatcat cggtacttgc gaaggtgcaa tactccagtt caaccgaaga
      901 agctgcccat tcaataataa aatcgatatc gctttgacct aaaagaccta ttcgcgaaaa
      961 tccccttttt tttgcctctt taacaacagt aacaataacc tctttataat caaccacctt
     1021 cttgatggtt cttttaagat accgataggt ccttccggct aattcgttaa taccatcagg
     1081 ggtaagtacg taggatacat ttcggggaga aattttttgg attatgataa aacccttttg
     1141 actgaggcgt ttaagaaggg cattggtcat acccagagac atatttatcg cacgggacaa
     1201 atccctctgg ctaatatctg cttgggattg agtgagatca tgaattgttt caagtagaag
     1261 aaattccgaa tcctgttcca tacctgcctg atgtttgttc aatctatgaa cagactatca
     1321 tacgagaggg gcattgacaa gataaaaccc caaaaggact taagggcatc acctctcaat
     1381 cttctttcgc aaagggagcc tgatgtggta tgattaaggt atagcagtat ttccaaggag
     1441 ctttttatgc cagttgcccc ctacatcaaa gatttaatga cttcttcatc ctttatcagg
     1501 cgaatgttcg aggaaggcat tcaaatgaaa actcagttcg gagccgataa ggtctacgat
     1561 tttagtatcg gaaatcccga tcttgagccg ccagcaaagg ttaaaacagt attacaacgg
     1621 cttgctgcct caactgagcc atcgttgcat ggatatatgc caaacgcggg atatcctcaa
     1681 acacgggcag ccatggcaca aaaggtttca atagagcagg gtattaccgt cgatttttcc
     1741 cgcattgtca tgggcgtagg agcggctggg gcgatgaact cagttcttaa ggcgattctt
     1801 tctccctcgg acgaagtctt ggtatcagcc ccgtttttcg ttgaatatac ccagtatgta
     1861 cgtaatcatg gggggacact catcacggtt cctgccaagg aagacttttc cctggatata
     1921 acggcaatag ccggggccct aacaagcaaa acctgcgcca ttattatcaa ttcacccaac
     1981 aatcccactg gaaagattta caccgaagat gaaataatag ctctagcccg tacgctggag
     2041 caacatcggg aaaaaaccgg aaaaactgtt cttataatcg cagatgaacc ctaccgggaa
     2101 attctctatg ataacaaaac agtcgctcca ctattccctc actggaagga ttcagtaatc
     2161 gttacctcct ttgccaaaaa cctgagtcta ccaggggaac gcattggcta caccgccatt
     2221 aatccctcat gtcctgatgc ccaagaatta accgatgcca taatttatgc aacaagggtc
     2281 cttggctatg taaatgcgcc tgcaacattc caacgagttg tagcagaatg ctggaatgaa
     2341 ccggtagact attcgttgta tacacaaaga cgtaatatct taacctcaat actcgataaa
     2401 gcaggtatat cctatgctgc tcctgagggt gctttctacc tgttttgcaa ggttccacaa
     2461 tgcctcagtc aacccgaccg ctctggtgat gatgccgctt tttgtgatca cttaaaaaaa
     2521 tatcgcattc tgggtgttcc tggacgtgga tttggaaaat ctggatggtt ccgccttgca
     2581 tactgtgtgt ctctagcgtc tgtgcaaaac tcggaagcag cgtttattca ag
//
LOCUS       DAOQXQ010000192         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42799_length_2631_cov_2.979037, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000192 DAOQXQ010000000
VERSION     DAOQXQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42799_length_2631_cov_2.979037"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    211..220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<221..1066)
                     /locus_tag="PKH81_04040"
     CDS             complement(<221..1066)
                     /locus_tag="PKH81_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ97240.1"
                     /translation="MSAFRISGYTEPDFMKHKRLFAHLISLLAVITLMVSCAHTRAHG
                     VNRIRHEDLAIPDLPNHCDDYKIAFISDIHYGNNFSRTRMERLVESVNAQKSDLIILG
                     GDLTRGTQDIKEFALLAGGFSAPHGVLAVLGNHDFYNGRSETVRALRKQGIIVLDETL
                     IETPAGVTVTGINDLRDIYPVTGRLRDILREDRVNILVSHNPDYAEEGDTAPFDLILS
                     GHTHGGQITIFGWAPVIPSRYCQKFRTGTVYTRGKPVIISNGAGYSGEVVHFRFFAPS
                     AYLIIT"
     gene            1148..1903
                     /locus_tag="PKH81_04045"
     CDS             1148..1903
                     /locus_tag="PKH81_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNQ97241.1"
                     /translation="MEFSRDYLIHYYEADATQCLSLPALVQYFEDSAILHSASKGLDL
                     EYYHRNNCGWMLLKWDITIERMPLFGQTVTVTTRVHSMKGFLADREFRMTSADGTHLA
                     SARSNWLLVDTVRRRPLRVPAEQYEKFGVSPADERSFVSIEETLPPAGDGELDGECKT
                     FPVKTVYSVIDTNGHVNKVRYITCAIDSLPESFSRLHTPVSIRVQYKKELAAGMNADL
                     RTSLSPLGTESRHGVFDGQDLCAAIDIRWIERT"
     gene            1980..2582
                     /locus_tag="PKH81_04050"
     CDS             1980..2582
                     /locus_tag="PKH81_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97242.1"
                     /translation="MKGHGVYLVSALLLVSSVLGSVFLSGCILDLDPSKAEHRKAVVT
                     FTIPDSAAVSSSRALASDQGFLYIQTGYTAETAVLYGPYTTVFKSVYRTVDILAGSYP
                     YMALIYLPSLPKTPLTAVIPATTDTESFLQALRERWKDDLSLLASASAVVLKDITLEV
                     GTENAVSAVLIPATTLTATVETPATIPSLAPGSGGNTSRT"
BASE COUNT          648 a          603 c          651 g          719 t
ORIGIN      
        1 tgttatgcct aaaaaggtca tggctaccga aagggcgagt accagtatcc aaaagggcca
       61 atattttgcc cgatcgggat cttttgtgaa gaaagatttc atggaatagc tccggaatat
      121 acaaaattcg ttaccggaat agtaacgatt ataagagtgt aaacctgtta ttgaacagct
      181 gtcgaggtgt tttttgtgcc ggtcagggtg nnnnnnnnnn ggtgatgatc aggtaagcgg
      241 agggcgcgaa aaaacggaaa tgaacaacct cgccgctata tcctgctcca ttggaaatga
      301 taaccggttt tccccttgtg tagacggttc ccgtacggaa tttttgacaa tagcgagagg
      361 ggataacggg agcccaacca aaaatcgtta tctgtccacc gtgggtgtgg ccgctcaaga
      421 taaggtcaaa gggagctgtg tccccttctt cggcatagtc cgggttatgg ctgaccagta
      481 tgttcaccct gtcttcccga agaatgtcgc gtaatcggcc cgttaccgga tagatgtccc
      541 gcaagtcgtt aatgccggtg acagtcaccc cggcgggtgt ttcgatgagt gtctcatcaa
      601 ggacgataat tccttgtttt ctgagcgccc gcaccgtttc tgatcggcca ttgtaaaagt
      661 cgtgattacc caatacagca agcaccccgt gtggagctga aaatcctccg gcaagaaggg
      721 caaattcctt tatgtcctgc gtcccccgag tcagatctcc tcccaggata atcagatcgc
      781 tcttctgggc attaaccgac tctacgagcc tttccatccg ggtacgagaa aagttattgc
      841 cataatgaat atcagagata aaggcgattt tatagtcgtc acagtgattg ggaagatcgg
      901 gaattgcgag gtcttcatgg cgaatacgat taaccccatg ggcacgagtg tgggcacaag
      961 aaaccatcag agtaataact gccagaagag aaatgagatg tgcgaatagt cgtttgtgtt
     1021 tcataaaatc aggttcagta tagcctgata tcctgaaagc gctcaagaca agtgctcttg
     1081 ccccgggttt ttatgtggga tacactgatt gccataaaat aaatagtaca ataaggagag
     1141 acagactatg gaattttccc gcgattatct aattcactat tatgaggctg atgcgactca
     1201 gtgtttgagc ttgccagccc tggttcagta ctttgaggat agtgctatat tgcatagcgc
     1261 ttccaagggc cttgatcttg aatattatca tcgtaataac tgtggctgga tgcttttaaa
     1321 atgggacatc actattgagc gaatgccctt atttggtcag acggttacgg taactacccg
     1381 ggttcactcc atgaaaggtt tcttagcaga tcgcgagttt cgaatgacaa gcgctgatgg
     1441 tactcatctg gcaagtgccc gttcaaactg gcttctcgtt gatacggtgc gtcggcgccc
     1501 cttacgtgtt ccagccgaac aatatgaaaa attcggggtg tctcccgctg atgagaggtc
     1561 attcgtcagt atagaagaaa ccttgccacc agctggcgat ggcgagctgg atggagaatg
     1621 caaaacgttt ccagtaaaaa cagtctacag tgttatcgac actaatgggc atgtaaacaa
     1681 ggttcgctat attacctgcg caatcgactc gcttcccgag tccttttccc gccttcatac
     1741 gcctgtttcg atacgcgttc agtataaaaa ggagcttgcg gccggtatga atgccgacct
     1801 gcgtacctca ctgtcccctc ttggaacgga aagccgtcat ggtgtgtttg atggtcaaga
     1861 tctttgtgct gcaattgaca ttcggtggat tgaacgaacg taagaaaatt ggaattcctg
     1921 tttgtaacat tgtatacttc ccatgagtaa ctgatatcat agtttctgag ggggtatgta
     1981 tgaaaggaca cggtgtctat ctggtttcag ctttattgtt ggtgtccagt gtccttggct
     2041 ccgtctttct ctccggctgt attctggatc tggatccttc caaggccgag catagaaagg
     2101 ccgtagttac ttttacgatt cctgattcgg cggccgtgtc gtcgagccgg gccctggctt
     2161 ctgatcaggg ttttttatat atccaaacag gttataccgc cgaaaccgcc gtgttatatg
     2221 gcccctatac caccgtgttt aagagcgtct accgcacggt tgatatcctt gcaggtagtt
     2281 atccctatat ggctttaata tatcttccgt ctcttcccaa aactccgtta actgccgtaa
     2341 ttccggcgac aacagacact gagtcctttt tacaggccct tcgtgagcgc tggaaggatg
     2401 atctttccct tttagcttcc gcctctgccg tggttcttaa ggatatcacc ctggaggtag
     2461 gtacggaaaa cgcggtaagc gcggtgttga ttcctgcgac tacattgacg gctacggtag
     2521 agactcctgc aactattcct tctttagccc cgggatcagg cggaaacacc agtagaactt
     2581 gaatctctta ccttgtttga tgcccttgga agacaggtct cagtgcaaga g
//
LOCUS       DAOQXQ010000193         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_42996_length_2623_cov_2.539330, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000193 DAOQXQ010000000
VERSION     DAOQXQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42996_length_2623_cov_2.539330"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..806
                     /locus_tag="PKH81_04055"
     CDS             9..806
                     /locus_tag="PKH81_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNQ97243.1"
                     /translation="MALAAWLLALDLGVSVFSWRLGIPHHHWFKAVTILAQRASWTLV
                     PLAGIAFTAPRKKDTRTIAIRIAFPLSAALTLLAGLASFSVLPSVTRIAFGNPDSLWI
                     ASNAYVSFLSFLSLIPDRRIAERATRRRTFAAIALVALFFASLPALLRIVSLDGNIAI
                     LAVSLTLSITIFISVSLAQRRNAKESTESVARRPDVLSPSVLSHLSAREIEIAKLLAE
                     GRTNAEIADTLCISTKTVETHISNIFRKTGVNNRVQLARVLLSDDQG"
     gene            950..1798
                     /locus_tag="PKH81_04060"
     CDS             950..1798
                     /locus_tag="PKH81_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNQ97244.1"
                     /translation="MFVFVSCDSAFKRTISELPPPPVEPGLERIEFPSAILGKTMALY
                     VYLPPGYSPEREWPVLYLLHGFSNNEIEWFEYHKLNEVADRLIEVGLIKPTIIIAPQM
                     DNSWGIDSGKPAMSGTSPKRALFNGQYESYFVKEVMTLAETRYHASRERSKRSLGGIS
                     MGGFASLHIGLRNPKLFARIGAHSPALRGSQIPDYFLYAKPRKMADVDPIALARTKNL
                     RGMKVFVDCGTDDSLFAGCKEFADVLLERKADVTFFSAPGAHNGSYWHPNLARYLRFY
                     AGNQTE"
     gene            1960..2148
                     /locus_tag="PKH81_04065"
     CDS             1960..2148
                     /locus_tag="PKH81_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97245.1"
                     /translation="MSQEFKPIDAKHCYTCIQWDGQRTWYAEKKVVKVDEKKEGQCRV
                     FHKKVKADSVCEHFFPIR"
     gene            complement(2233..>2623)
                     /locus_tag="PKH81_04070"
     CDS             complement(2233..>2623)
                     /locus_tag="PKH81_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97246.1"
                     /translation="QYISILDGQTLTEKDLNPYQIPVTKLMRSPFSKPAVSWIVEKPL
                     YEEKMHNLEQLLLPEYNHTALVTQSRVSPDGEAILEATFSLYRIERAQSEEAKLYILV
                     SLFGISLLLFLCMAGSILLNFLTDRVS"
BASE COUNT          642 a          689 c          608 g          684 t
ORIGIN      
        1 gcttccttct ggcactggcc gcctggcttc ttgcccttga cctcggagtc tcggttttct
       61 cctggcgact tggaataccc caccatcact ggtttaaagc agtcactatc ctggcgcaaa
      121 gggcctcctg gacactcgta ccgcttgccg gtatagcgtt taccgccccc cgcaaaaaag
      181 atacaagaac catagcgata cggattgcct ttccgctttc cgccgcactt accttactcg
      241 caggcctcgc ctcgttttct gttctccctt cggtaacccg gatcgcgttc ggtaatcccg
      301 actcgctctg gatcgcctca aacgcgtatg tttccttcct ttccttcctc tcccttatcc
      361 cggatagacg aatcgccgaa cgggctaccc gtcgcaggac cttcgctgca attgcccttg
      421 ttgcgttgtt tttcgcttct cttcccgcgc tccttcgcat agtctccctc gacggaaaca
      481 tcgccattct tgccgtctct ctgactttgt cgataaccat ctttatttca gtttcgcttg
      541 cccaacggcg aaacgcgaaa gagtctactg aaagcgtcgc gcgtcggccg gacgtgctct
      601 cgcccagcgt acttagtcac ctgagcgcgc gggaaatcga gatagcgaaa ctcctggccg
      661 agggcaggac gaacgcggaa atcgccgata cgctttgcat ctccacaaag accgtggaaa
      721 cgcacatatc caacatcttt cgaaaaaccg gtgtaaacaa tagagttcaa cttgcgagag
      781 ttcttctatc cgacgatcag ggttaaccct gaggcgagac gaatggacgc gattcagggt
      841 tttccccgat tgcagacggc gcgatctgtg cgtaccctgt gatgcaagga gtcctcatga
      901 ttattcacga caaaaaaaag caattgcgct ctcatgcatc tttgcaacat tgttcgtttt
      961 cgtctcgtgc gattctgctt ttaaacgtac gattagcgaa ttacctccac ccccggtaga
     1021 accgggactt gagcgaatcg agtttccaag cgctattctt ggtaagacga tggcgctcta
     1081 cgtgtatctt cccccggggt actcgcctga acgcgagtgg cccgtccttt atcttcttca
     1141 tggttttagc aacaacgaaa tagaatggtt cgaataccac aagctcaacg aggttgccga
     1201 caggcttatc gaggtgggtc ttataaaacc gacgattatc atcgcgccgc aaatggataa
     1261 tagctggggg atcgattcgg gaaaaccagc catgagcggg acttcgccga aacgggcgct
     1321 cttcaacggt caatacgagt cgtacttcgt caaggaagtt atgacccttg ccgaaacccg
     1381 ctatcacgcc tccagggagc gttccaaacg gagccttggg ggaatctcga tggggggctt
     1441 cgcttcgctc catatcggcc tccgcaatcc aaagctcttc gcgcgcatcg gcgctcatag
     1501 ccccgcgctc cgtggctcgc aaatccccga ttactttctc tatgccaaac ccagaaaaat
     1561 ggccgacgtc gacccgatag ctttggcacg gacgaaaaat cttcgcggta tgaaggtatt
     1621 cgtcgattgc ggcacggacg attccctttt tgcaggatgc aaggaattcg ccgacgttct
     1681 tttagaacgc aaagccgacg tgacgttctt ctctgcaccg ggggcacata acggctctta
     1741 ttggcacccg aacctcgcac ggtatcttcg tttttacgca gggaatcaga cggaataatg
     1801 tgactgttga agtgatgtcg atacaaacat ttcgacatca cttcgcgctc tcattctcta
     1861 aaatctctta attatacatt atataaaaac gtacgctgcg gaagaaaact cttcttgact
     1921 cacactcgta ttggtcgata ttctaagaac ggaggaaata tgagtcaaga attcaaacca
     1981 attgatgcga aacattgcta tacttgcata caatgggacg gacaacgcac gtggtatgca
     2041 gaaaaaaaag tagttaaggt tgatgaaaaa aaagaaggtc aatgccgggt ctttcataaa
     2101 aaagtaaaag ctgattcagt gtgcgaacac ttctttccta ttcgataatg taaacagtac
     2161 tagcaacatg ggcccgcgta tggatgatat acaccatgcg tgggctcatg tgttttttaa
     2221 attcgctcat tcttatgaca cacgatcagt gagaaaattt aatagtatgc tccctgccat
     2281 gcacaaaaat agtagtaatg aaattccaaa aagacttacc agtatgtaca gttttgcctc
     2341 ctcactctgt gcgcgctcga tgcggtaaag agagaaggtt gcttccagta tcgcttctcc
     2401 atcgggagaa acacgcgatt gagtaaccaa tgccgtatgg ttatactccg gaagtagtag
     2461 ttgctccagg ttgtgcattt tttcttcata gaggggcttt tcgactatcc atgatacagc
     2521 aggttttgaa aaaggtgatc gcataagttt tgtaactgga atctggtacg ggttaagatc
     2581 tttttctgtg agtgtctgcc catccaggat tgatatgtat tgt
//
LOCUS       DAOQXQ010000194         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43011_length_2622_cov_3.647838, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000194 DAOQXQ010000000
VERSION     DAOQXQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43011_length_2622_cov_3.647838"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKH81_04075"
     CDS             <1..490
                     /locus_tag="PKH81_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNQ97247.1"
                     /translation="NLGACLSPDNSWIFLQGLETLPLRMERHNQNALAVARWLSRHPK
                     VAWVRYPGLEGDPSYPVASSRFTHGFGGMVVFGIKGEGSISGRAKGERFINALSLFSN
                     LANVGDTKSLAIHPASTTHSQMTDKEQLDAGLPPDLVRLSIGIEHIDDIIADLEQALA
                     KA"
     gene            complement(476..1210)
                     /locus_tag="PKH81_04080"
     CDS             complement(476..1210)
                     /locus_tag="PKH81_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNQ97248.1"
                     /translation="MSDSLYFHRLALLTGEQTVQLLNSSRVFIFGLGGVGSWCAEGLV
                     RSGVGSVVLVDFDTICPTNVNRQLQATSQAIGKKKADELACRLSLIYPACHIESRSEC
                     FSNENAQFFDIRSDDYVIDAIDSLENKLDLIEYAYDKGARLFSSMGAARKLDPTKLRT
                     ADIWDTQGCPLARMVRQGLRRRGWNGHFTAVYSTEQALPVKTNTTEEAPINGSAVHVT
                     ATAGMILAGLVVQDAHSRSGTDLRLG"
     gene            complement(1244..2371)
                     /locus_tag="PKH81_04085"
     CDS             complement(1244..2371)
                     /locus_tag="PKH81_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97249.1"
                     /translation="MKKSIALVLTAGLLLSCSFHSNDGEPQQDKETITQEQPQETQKP
                     ETQTPATVLGVKNNRLVDIYGKPVVVRGYEMTMILWFETPGSIAENIEMTLDTMTDNM
                     IATGANAVRFLFPAGLRDGLTLEKIDHYIGKMATNHVIVYLSIFGERRSELDADGNSV
                     EYDYYDSPAFFGNPDIKKILDKYSQWLVLDASQECDFDDRERWERVSKEKIKEFRSYG
                     YTCPLTVMSNTYGRDLPALLSERAQRIVDSDPLKSIIFGWQAYWGDYTNRDTGDTSNW
                     YEYYYQMSLEEGIRQAALAPYPIQIGLDYLADAESYVDYRSAMKIAEETGMGWLWWNW
                     YPVWGGNNLSNDGSLESLTDFGREVIYTDENSVQNTAIKAN"
BASE COUNT          635 a          627 c          616 g          744 t
ORIGIN      
        1 aaatctgggc gcgtgtcttt ctccggataa ttcctggata tttcttcagg gtttggagac
       61 gctcccctta cgaatggagc ggcacaatca aaatgccctt gcggtagcac gttggcttag
      121 ccgtcatccc aaggtagcct gggttcgcta tccggggctt gaaggcgatc cttcctatcc
      181 agttgcttct tcccggttta cgcatgggtt tggaggaatg gtggtgtttg gcattaaggg
      241 agaaggttct atctctggta gagcaaaggg tgagcgtttt ataaacgccc tgtctctctt
      301 ttctaatctt gcaaacgttg gggataccaa gagccttgcg attcatccgg caagtacgac
      361 ccattcgcaa atgacggata aggaacagct tgatgctggc cttcctcccg atttagtacg
      421 gctctctatt ggcattgaac atattgacga tattattgcc gatttggaac aagctctagc
      481 caaggcgtaa gtctgtacct gaacgggagt gtgcgtcctg cacgacaagt ccggcaagaa
      541 tcatgccggc agtggcggta acatggacag cgctcccgtt tattggcgct tcttctgtgg
      601 tgttagtctt gaccggtaat gcttgttcgg tactgtacac cgcagtaaag tggccattcc
      661 aacctcgccg tctaagtccc tgacgtacca tgcgcgcaag cgggcagcct tgagtgtccc
      721 aaatatcggc ggttctgagc tttgttgggt caagcttacg tgcggccccc atggaagaaa
      781 agagtcgcgc ccctttatcg taggcgtatt caatcaagtc gagtttattt tccagactat
      841 cgatggcatc tattacatag tcatcactgc gaatatcaaa aaactgcgca ttctcgtttg
      901 aaaaacattc acttctactt tcgatgtgac aggcgggata aattagggaa agccggcaag
      961 ccaactcgtc tgcttttttt ttgccaatgg cttgtgaggt cgcctgaagc tgacggttta
     1021 cattcgttgg gcagatggtg tcaaaatcta ccaagacaac tgagccaaca ccggagcgaa
     1081 caagtccttc tgcgcaccag ctgccgactc ctcccagccc aaagataaaa acccgggaag
     1141 aattaagaag ttgaacagtt tgttccccgg ttaacaaggc cagacgatga aaatataaag
     1201 aatcactcat agtctggcta ttgtaaccgg gaaatagatt atgtcaattt gccttgatgg
     1261 ctgtattctg tacgctgttc tcgtcggtat agattacctc gcggccaaaa tcggttaagc
     1321 tttcaagcga gccgtcattg gagaggttgt tcccgcccca tacgggatac cagttccacc
     1381 acagccaacc cattcccgtt tcttctgcga tcttcatggc agatcggtaa tccacgtagg
     1441 attccgcgtc ggcaaggtag tcaagtccaa tttggattgg atagggggca agggctgctt
     1501 gtcggattcc ttcttccagt gacatctggt aatagtattc ataccaattt gatgtgtccc
     1561 cggtatccct gttcgtatag tcaccccaat acgcttgcca gccaaaaata atcgatttaa
     1621 gtggatcgct gtctacaatt cgctgtgccc gctccgatag gagcgctggc agatctcggc
     1681 cataggtatt agacatgacc gtaagcggac aggtataccc atagcttcgg aactccttga
     1741 ttttttcctt gcttactcgc tcccaccttt ctcgatcgtc aaaatcgcac tcctgggaag
     1801 cgtcaagtac gagccattgt gagtacttgt ccaatatttt tttaatatcc ggattcccaa
     1861 aaaatgccgg cgaatcatag tagtcatatt cgactgagtt accgtcagca tcaagttcag
     1921 atcttcgttc tccaaaaatt gaaaggtaca caataacatg attggtcgcc atctttccga
     1981 tgtagtgatc aatcttctcc agggtaaggc cgtcacgaag tcctgcgggg aataaaaagc
     2041 gaacggcgtt agctccagtg gcaatcatgt tgtcggtcat ggtgtccaag gtcatttcaa
     2101 tgttttccgc aatacttccg ggtgtttcaa accacaggat cattgtcatt tcataacctc
     2161 gtactactac tggttttcca tagatatcca caagacgatt attttttaca ccgagcacag
     2221 tcgcaggtgt ttgtgtttcc ggtttctggg tctcttgtgg ttgttcctgg gtaattgttt
     2281 ctttgtcttg ttgtggctct ccatcgttgg aatggaaact acaagataat aacagtcccg
     2341 cagttaatac aagggcaata gattttttca tctgttactc ctccaatctt tgagaaaaca
     2401 gccagctcag gtaattattg actcgcattg caaaggcatc gtggccgcag tcatagtaat
     2461 ataccgattg gattacgcgg ggtttgccag caatcgaaag agttctcact tgcgcgtata
     2521 agggctcaaa gataccgctt gaccagtttg gcttgtccat aagatttaat tactgctcgc
     2581 aggattgttg gtattttcct ccttttccca taaaaaagta ag
//
LOCUS       DAOQXQ010000195         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43019_length_2622_cov_2.895988, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000195 DAOQXQ010000000
VERSION     DAOQXQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43019_length_2622_cov_2.895988"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..791)
                     /gene="xseA"
                     /locus_tag="PKH81_04090"
                     /pseudo
     CDS             complement(1..791)
                     /gene="xseA"
                     /locus_tag="PKH81_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106426.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
     gene            861..2471
                     /locus_tag="PKH81_04095"
     CDS             861..2471
                     /locus_tag="PKH81_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HNQ97250.1"
                     /translation="MKNLVVLFAGEMSPHGTQSLCSGPSAFERSIAAAVYPGASRVLV
                     LISDTRYNGFPSPVSPSNIPEQLNDSLKTEVIHKPAWTPAALWERLSRESQGFDNVIF
                     QFADTPSIDPVLTAELIARHERYAAEYTFAEGYPVGLSPEILASGITQTLARLSAGQQ
                     GPVRRDGIFELIKKDINSFDIETIMSDQDMRHLRITLACDTRQGFLTCEALEGINGAN
                     AFKLANSRASLLRTRPAFYSIQVAGPCPVSCIHCPYPAYCLSGTSSSPKIDVRSRTDF
                     MDPNAFFTLVDRIASFSHNAVVSLSLWGECALHPDIPALIEKVLSYPSLSVLIETTGL
                     GWSQSSIEKIARIASASGPREQPVSPINWIVSLDACSASRWESLHTGLPPLSSADSLF
                     SQALHTMESLCSRFPDSVWPQMIRMKDNEEELELFYRSWKEKLSRVIIQKHDHFCSTI
                     QDRRVADLSPLVRRPCWHLGRDMSILIDGTVPLCREDLYAARSLGNALTDELEHIWNN
                     GAPVYEQHLCGNYEGMCGACDEFYTFNF"
BASE COUNT          638 a          621 c          632 g          731 t
ORIGIN      
        1 agaagttggg caaccagttc tgccgctgcc gagggggtag gggcccggac atcggcggca
       61 aaatcacaga gagaccagtc tatttcgtgt ccgacggcgc taacgaccgg tatggaagat
      121 gcggcaacag aacgaaccag atcttcgtcc gagaaaggaa gtaaatcttc cagagaccca
      181 ccgccgcggc caataataat aacatctccc attgaatgta gattcgctcg ggtaatggcc
      241 ttgattagct caggtgccgc ttctgcgccc tgtacgggac agcccagaat aataatgtct
      301 atctttgggt ttcttcggcg gattacctga ataatgtccc tgactgcagc tccggtggga
      361 ctggtaataa cgacaacctt ttaagggaaa aacggcaagg tttgttttga atcctcatca
      421 aacagtcctt ctgcggcaag gcgtcttttg cgatcttcaa gaagccgtag tatgtcaccg
      481 gaaccggcaa gcgacattga atcgactact aattggtagc tcccccgggc tgcatagaca
      541 gaaagtgagc ctgtcgcgtg aaccaacata ccatctgttg gtacaaaacc aagagagcga
      601 gattttccct tgaacattac tgcctggagt gcgcttgatt catcctttag ggtaaaatac
      661 aggtgtccgg tactggacgg ccggtaattc gagatttctc cttcaacggt aatgaccgga
      721 agggaagatt caagaatttc cttgattagg ctggttattt catggatttt gagaatttgg
      781 tcgccaggca taaaagtaca ctagcatgac ggggaagttt tctcaagcag agatagcggt
      841 gcgccgatac tggtaataga atgaagaacc ttgttgtcct ctttgccggg gaaatgtctc
      901 cccatggcac ccaatccctc tgttccgggc cttcagcctt tgaacgttca atagcagctg
      961 ctgtgtaccc gggggcctcc cgcgtgttgg tacttatctc cgatacgcgt tataacggtt
     1021 ttccgtctcc ggtatctcct tctaatattc cggaacaact gaacgattcc ctcaaaacag
     1081 aggtgattca caaacctgca tggacgcctg cggcgttgtg ggagcgactg tcccgcgaat
     1141 cacaggggtt tgacaacgtt atttttcagt ttgccgatac gccgagcatt gaccctgttt
     1201 taacagctga attgattgcc cgtcatgagc ggtatgcagc tgaatatacc tttgcagaag
     1261 gatatcctgt agggctttcg ccggaaatac tcgcctcggg aataactcaa acattagcac
     1321 gtctttctgc aggtcaacag ggacccgttc gtcgcgacgg catttttgaa ttgataaaaa
     1381 aggatattaa ttcatttgat attgaaacaa ttatgtctga tcaggacatg cgacatctac
     1441 gaattactct tgcctgtgat acccgacagg gatttttaac ctgcgaagct cttgaaggga
     1501 ttaacggagc aaatgctttc aagctcgcta attctcgcgc ttccttactc agaactcggc
     1561 ccgcctttta ttcgattcag gtcgcggggc cctgtcctgt ttcctgtatc cattgtccct
     1621 atccagccta ctgtcttagc ggtacctcct cttcaccaaa gatagacgta cgatcacgta
     1681 ctgattttat ggatccgaat gcttttttta ccctggtaga tcgaattgct tcgttttctc
     1741 ataatgcagt tgtgtcactt tctttgtggg gtgaatgtgc actgcatcca gatattcctg
     1801 ccttgattga aaaggttctt tcttatcctt cgttgtcagt tcttatagag acaaccggcc
     1861 ttggatggtc acagtcttcc atagaaaaaa ttgcccgaat cgcatctgca tcaggtccac
     1921 gcgaacagcc ggtatcaccg attaactgga ttgtatccct tgatgcgtgt tcggcctcac
     1981 gttgggaatc gttacatact gggctacctc cgctatcctc tgctgattca ttgttttctc
     2041 aggccctgca cacaatggaa agtctttgct cacggtttcc cgattcggtg tggccgcaaa
     2101 tgattcgtat gaaagacaat gaagaagaac ttgaattgtt ttatcgatcg tggaaagaga
     2161 agctttcccg ggtaataatt caaaaacacg atcatttttg ttcgacgatc caggatcggc
     2221 gtgttgccga tctttctccg ttggtcagac gaccctgttg gcatcttggg cgtgatatga
     2281 gtatactcat cgatggaacc gttcctctgt gccgggaaga cttgtacgcg gcccgttctt
     2341 tgggaaatgc tttaaccgac gaattagagc atatatggaa taacggcgct cccgtctatg
     2401 aacaacatct ttgcggcaac tatgaaggaa tgtgcggagc ctgtgatgaa ttctatacct
     2461 ttaattttta acgaacgtaa aattgcctat acgatagttg gtggtggtct caaacaaagt
     2521 gatgaagagt atagtcaggt aacggtagtg ctccttaatc ggggtggccg ctattatcgt
     2581 tcggctgtat ttcagaatct ggagaaagcc ggtttctcct ct
//
LOCUS       DAOQXQ010000196         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43103_length_2619_cov_2.554602, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000196 DAOQXQ010000000
VERSION     DAOQXQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43103_length_2619_cov_2.554602"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKH81_04100"
     CDS             <1..336
                     /locus_tag="PKH81_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNQ97251.1"
                     /translation="INGKSVDALSQLCFRGNAYDKARHVCEQLREEITRQQFKIAIQG
                     AIGSQVIARETVNALRKDVLAKCYGGDISRKRKLLEKQKEGKKRLKMVGDVELPQSAF
                     LAVLKTKEN"
     gene            complement(333..1964)
                     /locus_tag="PKH81_04105"
     CDS             complement(333..1964)
                     /locus_tag="PKH81_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNQ97252.1"
                     /translation="MSGTEGPLLVDTGRGHTVFFQGRHLYSRYDPARIPEETVTREHI
                     LPETLVLCISPVLGYGLEKLLASLPAGCAVVSVERDQELFNLSCTAIPQSVHQHPLFT
                     NQCVDICSNLETLIEDLPGFPFRRCVRLDLSAGSALYQDFYTSLTAHVDSLISVYWKN
                     RVTLMQLGRNYFRNFFINLALLPASSPLTPYSVHKAILILASGPSLLDGLHFARKHRD
                     MLFILAVDTAMPALREAGITPDACIILESQFWIEDSLLGWKNSRIPIFADLTALPRAI
                     GATGGPVSFFFTHFTRSHTLSRFMNTSFVPPTMQRLGSVALTTVSIARKIAESSLPVF
                     FAGLDFSWGNGYTHAQGTSPVVRIQNETNRIRSLALAGIKENTRKVSGKNGTPYTTDE
                     TLLRYAALCREQAAGMQGVFDIGQSGLSTGYPNLTLVEADALVASIPCRQDATCTTEP
                     QQPDKIDGKDIAAFLKAEQQRILEIRSRLTGEYQEHSDAEERTTLLSKIAECDYLFLH
                     FPDGHKGVGREQNFLNRVRVETEYFYKLFAQRVLG"
     gene            complement(1967..>2619)
                     /locus_tag="PKH81_04110"
     CDS             complement(1967..>2619)
                     /locus_tag="PKH81_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97253.1"
                     /translation="RSDLNALFAEAEHLQTVLSLQVGSISSPVKAGNSWALVRYDVPP
                     QKPDFTNQAVLNAVRDYMFKNERGMIEDFFTVSAKNFAEVARAKGFDAACAELKLEKK
                     TTAAFPLNWYNVEILPPVAREGELAPAAANETFLRTAFSLKPSEVSSPIMLGWNILVL
                     QLAEEKAADTQMTELIPMYYTNYAQAWAENSLSASFLSSSKLKDNFDKTYQQYFAN"
BASE COUNT          709 a          571 c          692 g          647 t
ORIGIN      
        1 atcaacggta agtcggttga cgctctttct caattgtgtt tcaggggaaa tgcgtacgac
       61 aaggcccgtc acgtatgtga gcaactaagg gaagaaataa ctcgccaaca attcaagata
      121 gcaattcagg gcgcaattgg aagccaggta attgcccgag aaaccgttaa cgcgctccga
      181 aaggacgttt tggcaaaatg ctatggcggt gatatatccc gtaaacgcaa actgttggaa
      241 aagcaaaaag agggaaagaa acggcttaag atggttggcg atgtggaatt gccgcaatcc
      301 gcctttcttg ctgttcttaa aaccaaagaa aactaaccaa gaactctttg tgcaaaaagc
      361 ttataaaaat actcagtctc cacacgtacc ctgttcaaga aattttgctc tcttcccaca
      421 cccttgtgtc catccgggaa atgaaggaat aagtaatcgc attccgctat cttcgaaagc
      481 agcgtagtgc gctcctctgc atcgctatgt tcctgatact cacccgtcaa acgggagcga
      541 atttccagga ttcgctgttg ttcagccttt agaaaggcgg caatgtcttt gccgtctatc
      601 ttgtccggtt gttgcggttc tgttgtgcaa gtggcatctt ggcgacaagg aatagaggct
      661 actagtgcat cggcttcgac aagcgtgagg ttggggtatc ctgtagacaa tcctgattga
      721 ccaatatcaa acacaccctg cataccagcg gcttgttctc tgcaaagagc tgcatagcgt
      781 aaaagggtct catcggtagt gtacggtgta ccgttttttc ctgatacctt acgggtattt
      841 tccttgattc ctgcaagggc caggctccta attcgattcg tctcgttttg tatacgcacg
      901 acagggcttg tcccttgtgc atgggtgtag ccattgcccc aggagaaatc aagaccggca
      961 aaaaaaacag gaagtgatga ttctgctatc tttcgtgcaa tggaaaccgt ggtgagcgca
     1021 accgagccca agcgttgcat tgtaggaggc acaaaggatg tgttcataaa acgggaaagc
     1081 gtatgtgagc gggtaaagtg ggtaaaaaag aatgaaacag gtccgccggt agctccaata
     1141 gcgcggggaa gggcagtcag gtcggcaaag atcgggattc gggagttttt ccatcctaac
     1201 agggaatctt cgatccaaaa ttgactttcc aaaatgatgc acgcatcggg ggtgatgccc
     1261 gcttctcgta atgcgggcat ggcggtgtct accgccaaaa taaagagcat gtccctatgc
     1321 ttgcgggcaa agtgtaaacc gtcaagaagc gatggcccag aggcaagaat tagaatagcc
     1381 ttatgtaccg agtaaggcgt aagaggtgag ctagcaggaa gcaatgccag attgataaag
     1441 aaattgcgaa agtaatttct tccaagctgc attaaggtta ccctgttttt ccagtagaca
     1501 gaaataaggg aatctacatg tgcggttagg gaggtgtaaa aatcctggta caaggcagag
     1561 cctgcggaaa ggtcgagccg tacacatcgc ctgaaaggaa aaccgggaag atcttctata
     1621 agcgtttcca gatttgaaca gatatcgacg cattgattgg tgaataaggg atgttgatgg
     1681 acgctctggg gaatagcggt gcaggacagg ttaaaaagtt cttggtcgcg ttcgaccgaa
     1741 acaacggcgc atccggccgg caaggacgca agcagttttt ctaatccata gccaagaaca
     1801 ggagatatgc ataatacaag ggtttctgga agtatgtgct cacgtgttac cgtttcttcc
     1861 ggaatacgtg ccggatcata gcgggaatac agatggcggc cctggaagaa aacagtgtgt
     1921 cctctcccgg tatctaccag gagaggtccc tcagtgccgc tcaccgttaa ttagcaaaat
     1981 actgctgata tgttttatcg aaattatcct tcagcttgct gcttgataaa aaggaagctg
     2041 aaagactgtt ttcggcccat gcttgcgcat aattggtata gtacatggga atgagctcgg
     2101 tcatttgcgt atctgcggcc ttttcttcgg ctaattgcag gacaaggata ttccatccca
     2161 gcattattgg tgaggaaacc tcggagggtt tgagtgaaaa agcggttcgc aggaaggttt
     2221 catttgctgc tgctggagca agctctcctt cgcgtgcaac cgggggaaga atctctacgt
     2281 tataccagtt aagtggaaac gcagcggttg tttttttctc cagcttcaac tctgcgcatg
     2341 ccgcatcaaa tccctttgcc cgagctactt cggcgaagtt tttggcagac acggtaaaga
     2401 aatcttcgat catgccgcgt tcattcttga acatatagtc gcgaactgcg ttgagtacgg
     2461 cttggttggt aaaatcgggt ttttgaggag gaacgtcata gcgaacgagg gcccaggaat
     2521 tacccgcttt taccggagag gagatagatc ccacctgaag agacaagact gtttgtaagt
     2581 gttcagcctc ggcgaacagg gcgttaaggt cgctgcgca
//
LOCUS       DAOQXQ010000197         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43239_length_2613_cov_3.637217, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000197 DAOQXQ010000000
VERSION     DAOQXQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43239_length_2613_cov_3.637217"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PKH81_04115"
     CDS             <1..1173
                     /locus_tag="PKH81_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNQ97254.1"
                     /translation="GEGGAGTFSDGKLYTRSNKRGDISRILSILHHHGADASICTEAH
                     PHIGSDLLPGIINAIRATIVSHGGIFKFNTRCTNFIHHKGILKGIIATSVDHSEEILC
                     DCVILASGHSARDIYHILSDLENDEGIQGPLLEAKGFAMGVRIEHQRDHIDAIQYHGK
                     RSQLDLPAASYRLSAQIQERGVYSFCMCPGGLIVPSMTEDGLLCVNGMSPSGRNSRWS
                     NAAIVVEIRSEDIPTGKDCEKDALRGLYYQMEFEREAARQGNKAAAPAQRLVDFIDSK
                     QSTSLPESSYSPGLVSSRLDQWLPAHILSRLAPACMEFNRFMKGFISPEAILVAPETR
                     TSAPVRILRDPQTCESPALSGLFPAGEGSGYAGGIASSAMDGEKAARAVLAKKHWQ"
     gene            complement(1175..>2613)
                     /locus_tag="PKH81_04120"
     CDS             complement(1175..>2613)
                     /locus_tag="PKH81_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNQ97255.1"
                     /translation="PDRPAFFNPIYRKNCTLTFELTSHQAFDVNSLVSALATSVIIFI
                     LAWYSERRQEALVARLTDMLVFDPTTGLPNKEALCHSIQSDKGYVFAIIKIENFSNLV
                     ALFGYEFSDIISQFASQKLRKYEHRYGYTTYQLKYNEYGILIETSVNVTIADASQRLS
                     DVVQSLELESLPWERDRIKLVYRVGGALVSPGDCETPLSRADVALKKAERGHSVICIF
                     ENDNTEKESAYIYAIRFTELINNRENHSFKAVFQPVFSSDGKEIVWYEALLRIKQKNG
                     EYSSIYPYLDVAKSTGFYQYLTDFILRKSAEAICEFDVDISINISITDIVRPAFITLV
                     DEVYSLIRHKQGKIIFEILESDELVELDKCIWFIDYISHYGFKIAIDDFGTGYSNYCN
                     LINLPIDIVKIDGSLIRKIKTDDNARLLVEGIIHFCKKSNKKTVAEFVEDIQVFESLR
                     NMEIDFLQGFYLSEPAPISECPCPTILP"
BASE COUNT          788 a          597 c          535 g          693 t
ORIGIN      
        1 ggagagggag gagcgggaac cttctcggac ggtaaactat acacacgttc aaacaaacgt
       61 ggagacatct cgcgcattct ttcaatcctt catcatcacg gagcagatgc ctctatctgt
      121 accgaagctc atcctcatat aggaagcgat ctgttaccag gtattatcaa cgctatccgt
      181 gccacaattg tcagtcacgg cggaatcttc aagtttaaca cccgatgcac caactttata
      241 caccacaaag gtattctcaa aggtataatt gcaacctcag tagatcactc cgaagaaatt
      301 ctgtgcgatt gtgttatttt ggcttctgga cactctgcgc gcgacatcta tcacattcta
      361 tctgatcttg aaaatgacga gggtatacaa ggccctctac ttgaggcaaa aggctttgct
      421 atgggagtgc ggattgaaca ccaaagagac catatcgatg caattcaata tcatggcaaa
      481 cgatcccagc tcgatctacc tgcagcaagc taccgtctct ctgcacaaat tcaagaaaga
      541 ggggtgtact cgttctgcat gtgtcccgga ggactcatcg ttcccagcat gaccgaggac
      601 ggactgctct gtgtaaatgg aatgtcccct tctggaagaa actctcgttg gtcaaatgcg
      661 gctatagtag ttgagattcg aagcgaagat attccgacag ggaaagactg cgaaaaagat
      721 gccctacgag ggctttacta tcaaatggaa tttgagcgag aggctgcaag acaagggaat
      781 aaggcagcag ctccagcaca acgccttgtt gactttatag actcaaaaca gagtacaagc
      841 ctcccggaat catcctacag cccaggctta gtatcttccc gtttagatca atggcttccc
      901 gctcatatac taagtcgcct cgcccctgca tgtatggagt ttaatcgatt catgaagggc
      961 tttatctctc ccgaagctat tcttgttgcg ccggaaacca gaacatctgc tccagttcgg
     1021 attcttaggg atccacagac ctgcgaatcg ccagctcttt cagggctttt cccggcaggg
     1081 gaaggctccg gatacgcagg gggcatcgca tcttcggcaa tggatggaga aaaggccgca
     1141 cgagcggtgt tggcgaaaaa acactggcaa taattcaggg aagtatcgtg gggcagggac
     1201 actcagaaat tggcgccggc tcagacagat aaaatccctg taaaaaatct atttccatat
     1261 ttctcaacga ctcaaacact tgaatatctt ctacaaactc cgcaacagtt tttttgttgg
     1321 acttcttgca aaaatgaata atgccctcta ccaaaagacg ggcattatca tccgttttta
     1381 tcttgcgaat aagagaacca tcaatcttga ctatatcaat gggaagattt atcaaattgc
     1441 aataatttga atagcccgta ccaaaatcat caatggcaat cttaaaccca taatgtgata
     1501 tatagtctat aaaccagatg catttatcca actcgaccaa ttcatcactt tccagaattt
     1561 caaaaattat ttttccttgt ttatgtcgaa tcaatgaata cacttcatca accaaggtga
     1621 taaatgctgg ccgaacaata tctgtaatag aaatattgat tgagatatca acatcgaatt
     1681 cacatatcgc ttctgcggat ttacgtaaaa taaaatcagt caaatactga taaaaccctg
     1741 tcgattttgc cacatccaaa taggggtaaa tcgaagaata ctctccattt ttttgtttta
     1801 ttcgtaacag cgcttcatac cagacaatct cttttccatc agaggaaaaa accggttgaa
     1861 acacggcctt aaagctgtgg ttttcgcgat tgttaatcaa ctcggtaaat cgaattgcat
     1921 aaatataggc gctttctttc tctgtattat cattttcaaa aatacagatt accgaatgcc
     1981 cgcgctctgc tttcttgagt gcaacatcgg cacgagatag cggggtctcg caatctcccg
     2041 ggcttaccag ggctccaccc acccgatata ccaatttaat tctatcccgt tcccatggaa
     2101 gagactctag ctcaagggat tgaacaacat cactcaagcg ctgagaagca tcagcgatcg
     2161 ttacattcac ggatgtttca attaaaatac catattcatt atacttaagc tgataggtgg
     2221 tatacccgta gcgatgttca tactttctca atttttgtga tgcaaactgc gaaataatat
     2281 cagaaaactc atagccaaac agagctacca gattactgaa gttttcaatt ttgataatag
     2341 caaatacata acccttatcg ctttgaatac tatggcacag ggcttcctta tttggcaaac
     2401 cagtagttgg atcaaaaacc agcatgtcgg taagacgagc gacgagtgct tcctggcgac
     2461 gctctgaata ccaggcaaga ataaaaataa ttacagaggt tgcaagggcg gatacaaggg
     2521 aatttacatc gaatgcctga tgggacgtta gttcaaaggt tagtgtacaa ttctttctgt
     2581 aaattgggtt gaaaaaagca ggccgatcgg gct
//
LOCUS       DAOQXQ010000198         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43638_length_2599_cov_2.312107, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000198 DAOQXQ010000000
VERSION     DAOQXQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43638_length_2599_cov_2.312107"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKH81_04125"
     CDS             <1..303
                     /locus_tag="PKH81_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97256.1"
                     /translation="VPFNAENVTSTYVIRSGYSRMILLFLFAIGGLAVLSYLIQGYTR
                     GIREYRGAALGLVSFLLGYFLLGVCDSWLFFVCGAALLMQGSILYLKRIHAYYLWQ"
     gene            complement(316..954)
                     /locus_tag="PKH81_04130"
     CDS             complement(316..954)
                     /locus_tag="PKH81_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HNQ97257.1"
                     /translation="MKGSLMGLLSEFRYRKISQTATVNDLLKEFFRKTIHICASLVPF
                     LASWHYSLTITALCLVTLAYVYCEYNRMRGRPIPFVSRITAYASRRRDDGRFVLGPVT
                     LSLGILFTLLLFPPSAARVGIFALAFGDGVASLAGKIYGRIKIPGARGKTLEGSSACL
                     IAVYASTMAVTHNPVIALEAALFAMLIEVLPLKDYDNFFIPLAISSLVVLIG"
     gene            complement(990..1061)
                     /locus_tag="PKH81_04135"
     tRNA            complement(990..1061)
                     /locus_tag="PKH81_04135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Val,seq:cac)
     gene            complement(1162..>2599)
                     /locus_tag="PKH81_04140"
     CDS             complement(1162..>2599)
                     /locus_tag="PKH81_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97258.1"
                     /translation="ERLLNRYKNDLPAGLEYAEMLRTDLRNFEKAETVLRRRVLDYHV
                     NNQEGLMLLGDNFLDWADEDPSKYPLARQAYATLIELYGSKDPFLARMMRYFIRTDNL
                     AEVLPLKEHFTGRKAKIDASDLVELSGYLLEKRYTPKPGESEGLRAQIEDLRSLLERA
                     VKADPEVPEAHYNMGRFFLFNYKDDLASTALEESLRLFDQARTMSPRRILTHIDSYRL
                     LGEIRSSEKEYLKAQALLGEGIALYEKQRENRGVRPDPLVGKLYSDYADIDYFISNDL
                     ERSLENYQKAVTELNDTPSIRYRIGYIHYRNQDWESAMESFSLGYARAPSDVNLAYSF
                     GNTLFRKGNLYLAQGHYERLMEKLEAEKIRKGIVFPQTRVDHGSFVQQYMRAANNLGV
                     TLNRLAVRTGDSRKNARAMTLLSESTRAWDALTRNPTTMLRTQDKNLALANITYATHP
                     LPDFSPEIYADIPKTLDGEPVLQQREDR"
BASE COUNT          671 a          617 c          621 g          690 t
ORIGIN      
        1 gttcccttta acgcagagaa cgttacgtca acctatgtta ttcgctcagg atattcccga
       61 atgattctcc tttttttatt cgcgatcgga ggccttgcgg ttctttcgta tctaatacag
      121 gggtacacga ggggtattcg tgaatatcgg ggcgcggcgt tggggcttgt ctcttttttg
      181 ctcggttatt ttttgctggg ggtgtgcgac tcttggctgt tttttgtctg tggcgcagct
      241 ttgttaatgc agggatccat tctctatctt aaacgcatcc atgcctatta cttgtggcaa
      301 taaaaattaa cggtattacc cgatcaacac caccaatgaa ctgattgcta agggtataaa
      361 aaaattatca taatctttaa gcggtaagac ctctatcaac atggcaaaca aggccgcttc
      421 aagggcgata acaggattgt gggttactgc catggtagat gcgtacaccg caataaggca
      481 agcggagctt ccctccagtg ttttccccct cgcccctggt attttaatcc gcccatatat
      541 ctttcctgcc aaactcgcca ccccatctcc aaaggcaagc gcaaatatac caacccgcgc
      601 ggcagaaggt ggaaacagaa gcagggtaaa aaggattccc agactcaatg ttaccggtcc
      661 cagaacaaat cgaccatcat ctcgacgacg ggatgcatat gcggtaatcc gggaaacaaa
      721 gggaatcggg cgacctcgca tccggttata ttcgcaataa acataggcaa gagttacgag
      781 gcataacgcg gtaatagtta gggagtaatg ccaggaagca agaaagggaa ccaacgaggc
      841 acagatatgt atggttttcc ggaaaaattc ttttaacagg tcgttgaccg tagcggtctg
      901 cgatatcttt cgatagcgga attccgataa taatcccatc aacgagcctt tcattaccct
      961 cagtataaca taattaaaat gccctttatt ggacgttact ggatttgaac cagtgacttc
     1021 taccgtgtga aggtagcact ctaccactga gttaaacgtc caaaaaaggg cattatcacg
     1081 gccacggtta gtggccgaat gtatactaac aagagcggtt aaaaagtgca agggaagctc
     1141 ttggtcaatt gttttgtcgg cttagcgatc ctcgcgttgc tgaagaaccg gctccccatc
     1201 aagtgttttg gggatatccg cataaatctc cggtgaaaaa tcgggaagcg gatgagttgc
     1261 gtacgtaatg ttcgccaggg caagattttt gtcctgcgtt ctgagcatag tagtcggatt
     1321 tcttgttaag gcatcccatg ccctggttga ttcggaaagc aaggtcatcg ctcgtgcgtt
     1381 tttacgtgaa tcgccagttc gtaccgccag gcgattaagg gttaccccta gattattcgc
     1441 tgccctcatg tattgttgga cgaacgatcc atgatcaacc cgggtttgcg ggaagacgat
     1501 acccttacga atcttctctg cttcaagctt ttccatgagc cgctcataat ggccctgcgc
     1561 aaggtaaaga ttccccttgc ggaacaaggt gttgccaaag ctgtaggcaa ggtttacatc
     1621 cgagggagcg cgggcatagc ccaaactgaa cgactccatt gccgattccc aatcctggtt
     1681 gcggtaatgg atatacccga ttcgataccg tatcgaggga gtatcgttaa gctcggtaac
     1741 cgctttttgg taattctcaa gagaacgttc caaatcattc gagataaaat aatcgatgtc
     1801 ggcgtaatca ctatagagct tgcctacgag cggatccggt cgaacgcccc ggttctcgcg
     1861 ttgtttttcg tataaggcta tcccctcgcc cagcaaggcc tgagccttaa gatattcctt
     1921 ctccgatgaa cgtatttcac ccagcaaacg ataagaatcg atgtgtgtaa gaattctgcg
     1981 tggactcatt gtgcgtgcct gatcaaagag gcggagagac tcttcaagag ctgtagaagc
     2041 aagatcatcc ttgtaattaa acaagaaaaa acggcccata ttataatgtg cttctggaac
     2101 ctctggatcc gccttaacag cccgctccag taaggagcgt aaatcttcta tctgggcgcg
     2161 tagtccttca gactcgccgg gttttggtgt gtatcgtttc tccaataaat agccactgag
     2221 ctcaaccaga tcgcttgcat cgatttttgc cttgcgtccg gtaaaatgct ccttaagggg
     2281 taatacctcg gccagattat ctgttctgat aaaatagcgc atcatgcggg caaggaaggg
     2341 atccttgctt ccatacaatt caataagagt cgcgtatgcc tgtcgggcaa gcgggtattt
     2401 ggaaggatct tcatcagccc aatcaagaaa gttgtctccc aggagcatca aaccttcctg
     2461 attgttcaca tggtagtcaa gaacgcgtcg gcgtaatacc gtttccgcct tctcgaagtt
     2521 ccgtaaatca gtcctgagca tttcggcata ttcaagaccg gcgggtaaat cattcttgta
     2581 gcggttgagc agtcgctca
//
LOCUS       DAOQXQ010000199         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_43918_length_2588_cov_2.490722, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000199 DAOQXQ010000000
VERSION     DAOQXQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43918_length_2588_cov_2.490722"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..>2588)
                     /locus_tag="PKH81_04145"
     CDS             complement(166..>2588)
                     /locus_tag="PKH81_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97259.1"
                     /translation="YKIIMKTARETTTIITIEDIHWSDAVSLKMLEYLINGSDSAPLM
                     VIPVSRDAEHIQSFFSKYSIQTIKLNQLKAEDARHLAECLLRMRPVEEKLVEKIITTS
                     DCNPFFIENIVQNMIESGVLVEATDGTYFLSKDIKSINIPSNIQNIILSRLNSLLFEE
                     QVVCKTASVIGRTFMADILRALVPDGITEYIFKNALENFETHNLILREDERQASYYFK
                     HITIRDVVYNTILESTRKELNMMLLSYLESRFRDNLSSVVERLEYHATEAEAWPLVYK
                     YALQAAIKAEKQFSAQDALSHYTQAGEALRKIDIENKEEKEKEVALALASVYRRATEY
                     SRSLDLYKAMLEEEKRPLKRAAIFQGMGQCYQEQGNFNQAVEVLETALSLLGYRAPRG
                     SAHTYLSIGAEVLRQLGNWLVFRNNIRENKGDKRTISEMRSDILGILTKLYYFGKPEK
                     IAWASISNFNNSLAIRSSAEKWVVSESNYAVSLVSIGITNQGLAHFQNAEQAALSSKS
                     RTAQSIFKSRYAYFFLFYNQCEKSIELLEDATNHFRSIGEMWELMTAVGALAQNYFLI
                     TALDKSEKAYLETERVARKLNSPMHIGWAYNKVPFIRYLQGRMNAESARAMLSEGIHM
                     SQEVQDHMTLCIHYGHLASIAVMEGEVSAALNAAYSILEENRLYTVNIPHVKISFVNA
                     VEAFCFALESGMSGHERTKILSLARKTLAKALRLSATFQMMKGPSLRAASRLAFIEGK
                     MEKARKYCVQALEAFEHTPYEWEVAETLVFAGRLGMEKSAEWSKKGALLLQKNGIVAS
                     NKGYKRHT"
BASE COUNT          694 a          622 c          540 g          732 t
ORIGIN      
        1 aaggaacgag cctgccgagc ttggtgatgg tataaaacgc aacctctccg gcaacaagaa
       61 gagagccata aatacaaaaa cgaaacaaag cttgcctgat tcgcttccag ggcgatttaa
      121 gatgatcgta gcgcactttc ttttctctca tcgggaaacc tccgtttatg tatgtctttt
      181 atatcctttg ttgctggcaa caattccgtt tttttgtaaa agaagcgcgc ccttttttga
      241 ccattcggcg gatttctcca tgccaaggcg gccggcaaag accaaggttt cagcgacctc
      301 ccattcatag ggggtgtgtt caaaggcttc aagagcttgc acgcaatact tgcgggcctt
      361 ctccattttc ccttcgatga acgcaagacg tgatgcggcg cgaaggctcg gacctttcat
      421 catctggaag gtagccgaaa gccgtaaagc cttagctaat gttttacgcg ccagggacag
      481 aatctttgtc cgctcatgtc cgctcatccc tgactcaagg gcaaaacaaa aggcttcaac
      541 cgcattaaca aacgaaatct ttacatgggg aatattaacg gtatacagcc tgttttcttc
      601 aaggattgaa tacgctgcgt taagggcggc ggatacttct ccttccataa cagcaataga
      661 agcaaggtgc ccataatgga tacacaaggt catatgatct tgcacttctt ggctcatgtg
      721 tatgccttcg ctcaacattg cccgggctga ttcagcgttc atccgtcctt gaagatatcg
      781 tataaaaggc accttgttgt atgcccaccc aatgtgcatc ggtgaattaa gtttacgcgc
      841 tacccgctcc gtttccaaat acgctttttc ggatttatcc agggcggtaa tcaagaaata
      901 attctgggca agcgcaccaa ccgccgtcat cagttcccac atttctccaa tcgagcggaa
      961 atggttcgtc gcatcttcca acaactcaat tgatttttca cactggttat agaataaaaa
     1021 gaaatacgca taacgggatt tgaatatcga ttgggcagta cggctttttg atgaaagggc
     1081 tgcttgctcg gcattttgaa aatgcgcaag cccctgattg gtaataccga tactcaccaa
     1141 agaaaccgca taattacttt cgctcactac ccatttttcc gcacttgagc gaatggccag
     1201 gctgttgtta aagttcgaaa tacttgccca ggcaatcttt tcaggctttc caaaataata
     1261 cagtttggta agtattccaa ggatatcgct tctcatctcg ctgatagtcc gcttgtcacc
     1321 cttgttctcc cgaatgttgt ttctaaagac aagccaatta cccagctgcc tgagcacctc
     1381 ggctccaatg cttaggtagg tatgggcgct tccacggggc gcacgataac cgagcaagga
     1441 cagggctgtt tccaggacct ctaccgcctg attaaaattc ccctgctcct gataacactg
     1501 tcccatcccc tgaaaaatag cggctctttt aagtggtcgt ttttcttctt caagcatggc
     1561 tttatataaa tcaagcgacc ttgaatactc agttgccctt cggtataccg aagccagggc
     1621 taaggccact tccttttctt tctcttcttt attttcaatg tcgatttttc gcagcgcttc
     1681 ccctgcctgc gtataatgcg acaaggcatc ctgggcagaa aattgctttt ctgcttttat
     1741 cgccgcctgc aaggcgtatt tatacaccaa gggccaagcc tcggcctctg tcgcatgata
     1801 ctcaagccgt tcaaccacgg aagaaagatt gtccctaaaa cggctttcca aataactgag
     1861 gagcatcata ttaagctctt tacgagtgct ctcaagtatg gtgttgtaca ctacatctcg
     1921 aatggttatg tgcttaaaat agtatgaagc ttgccgctca tcctcacgga gtatcaaatt
     1981 atgcgtttca aaattttcaa gagcgttctt aaagatatat tcagtaatac cgtccggaac
     2041 caaagcgcga agaatatcgg ccataaaggt cctgccgatt accgatgctg ttttacaaac
     2101 aacctgttct tcaaacaaga gggagttaag acgagaaaga ataatgtttt gtatgttgga
     2161 aggaatattg atactcttaa tatccttgga aagaaaatac gttccatcgg ttgcttctac
     2221 aagaacacca ctttcaatca tattctgtac aatgttctca ataaaaaacg gattacaatc
     2281 cgaggtggta ataatctttt ctaccagttt ttcctcaacg ggtctcatgc gcaataaaca
     2341 ttctgcaaga tggcgagcat cctctgcctt taattgatta agcttaattg tttgaatgga
     2401 atacttggaa aagaaggatt gaatatgctc ggcgtcccgg gataccggaa taaccatcaa
     2461 aggagcgctg tccgatccgt tgatcagata ctcaagcatt ttaagggaca ccgcatcact
     2521 ccaatgaata tcttcgatgg taatgatggt cgtcgtttcg cgtgctgttt tcatgatgat
     2581 tttgtaca
//
LOCUS       DAOQXQ010000200         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_44035_length_2584_cov_2.693159, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000200 DAOQXQ010000000
VERSION     DAOQXQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44035_length_2584_cov_2.693159"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="sbcD"
                     /locus_tag="PKH81_04150"
     CDS             complement(<1..671)
                     /gene="sbcD"
                     /locus_tag="PKH81_04150"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HNQ97260.1"
                     /translation="MKLLHTGDLHLGKTLHEASLLEDQAVMLNQILARLGEDGYDALL
                     ISGDIYDRTIPPAPAVELFSDFLVHLTRDFPDLHTFIIPGNHDSARRLSFADRILGEK
                     HIHIIGNPEQTFSPINITLKNGHRTAFFLLPFLSSLSLTALSGPAVESTSGEFDFNAM
                     PQEASPLVSQADLAREAARRLSPLVTRLAQEGVPSILLAHLFTTGGVESESERVFLGT
                     AERVSP"
     gene            745..2454
                     /locus_tag="PKH81_04155"
     CDS             745..2454
                     /locus_tag="PKH81_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97261.1"
                     /translation="MKTRSLRIPLFFASVFFLLCCTAGVFFLTVAPARSRQADFDNLV
                     TLDSRLAELQIVLYRSVLAPVDTPRSAIEPLLDVIEASLASFSRQAMSKRHPLFPSSG
                     FATLEAIKTEVVDGTRTLLWCACDDASLVSTSTSIATVRSSLETLMPVIQQSLNRWKI
                     LTFIVAGSIIIGTWFLGLLSVWVLSVSVSRSSRRVGRVLEALSSGDYSACEEEIHAQE
                     SELSENLCAFAKKLKTMSETIAVEVVSNRTIGLSLSESLDNTSSTFEVVDGFIDSIRG
                     DVSVLEEQVSIVKKGLERITGGLDHLDDGIINQKTVVEGSLESVRGMITTIGSMADLA
                     IRDEKVIHDLVESSAQSQEIFAATYQKIILIGDSVSRINGMAEIIENIEEQTSMLSLN
                     AAIEAAHAGDAGKGFAVVAEEITKLAEASSESSREISSSISEIIEGITSMAKSSGLLD
                     EAFTQMTVNIESVRQTFSGFSEGLRTSRSDTQGVLTTMNTLQEVSSDVTRDSALVSDG
                     AGDIASSMNELDMLSSRVFDGITAMSLMLDGLKEVMTQFRTYADGIIASGNNLASHIP
                     QLK"
BASE COUNT          627 a          592 c          669 g          696 t
ORIGIN      
        1 ggggataccc gctcagccgt tccaaggaaa acccgctccg attctgattc aacgcctccg
       61 gtggtaaaca gatgagccag caaaattgaa gggactcctt cttgtgcaag acgggttacc
      121 agggggctaa gccgacgggc tgcctctcgc gcaagatcgg cctgggaaac cagaggagag
      181 gcctcctgcg gcatggcgtt aaaatcaaat tcgccggaag tggactcgac ggcaggccca
      241 cttagggcgg taagcgagag agaggacaga aaagggagaa gaaagaaggc tgtacgatga
      301 ccgtttttta gggttatatt tataggagag aaggtttgct ccggattacc gataatatgt
      361 atgtgctttt ccccgagaat tcggtctgca aaggaaaggc ggcgcgctga atcgtggttt
      421 cccgggatga taaaggtgtg aagatcgggg aaatcccggg tcaaatgaac cagaaaatcg
      481 ctaaaaagct cgacggcggg agcgggtggt atggttctat cgtagatgtc cccgctgatc
      541 aggagggcgt catagccatc ctcgcccaaa cgggcaagga tttggttcag catgacagcc
      601 tgatcctcaa gaagcgaggc ctcatgaagg gttttaccca gatgtaggtc cccggtatgg
      661 agcagtttca tatcgtatac tgtaagaaga tttattcgct gtgtaaagtg cttgtctaat
      721 tcgcttgtaa ggaaggtgta ctacttgaaa actcgctcgt tgcgcatacc gctttttttt
      781 gcctctgttt ttttcctgct gtgttgcacg gctggtgttt tttttcttac tgtcgcgcct
      841 gccagaagcc ggcaagctga ttttgataac cttgttaccc ttgatagtcg tcttgctgaa
      901 cttcaaatag tattgtatcg atcggttctc gctcctgtgg acacaccacg ctctgcgatt
      961 gagccactcc ttgatgtcat tgaggcatct cttgcttctt tttcccggca ggctatgtcc
     1021 aaaagacacc ccttgtttcc ttcttcgggc tttgctaccc tagaagcgat taaaacagag
     1081 gtggtcgatg gtacccgaac ccttctctgg tgcgcctgcg atgacgcatc ccttgtttct
     1141 acctccacat caatagctac cgttcgctct tctctggaaa ccctgatgcc ggtaattcag
     1201 caaagcctta accgatggaa aattctgacg tttatcgtgg ccggttcaat aatcatcggt
     1261 acctggtttc ttggtttgtt atcggtctgg gtgctttctg tctctgtgtc ccgcagttca
     1321 cgccgggtag ggcgtgtgtt ggaagcgctt tcttcaggcg attattctgc gtgcgaggaa
     1381 gaaatccatg cccaggaatc tgaattgtcc gagaacctgt gtgcctttgc caagaagtta
     1441 aaaacgatga gcgagactat agcggtagaa gtagtctcta accgcactat cgggctaagc
     1501 ctttcggaat ctctggataa tacctcttcg acgtttgagg ttgtagatgg atttatcgac
     1561 agcattcgag gcgacgtttc ggtattggaa gagcaggttt cgattgttaa gaaagggctt
     1621 gaacgaataa ccggcgggct tgatcatctt gatgacggga ttatcaatca aaaaacagtg
     1681 gtagagggct ctcttgaatc ggttcggggg atgattacca cgattggttc aatggccgat
     1741 ttggctattc gcgatgaaaa ggttatccat gacctggttg aatcttctgc ccaaagccag
     1801 gagatattcg ctgcaaccta tcaaaaaatc attcttattg gcgacagcgt ctctcgcatc
     1861 aacggaatgg cggaaattat cgaaaacatc gaagagcaga cgagcatgtt atccttgaac
     1921 gctgctattg aagccgccca tgccggagat gccggtaagg gatttgcggt tgttgccgaa
     1981 gagattacca agcttgccga ggcaagttcg gaaagttccc gagagatttc ctcttcaatc
     2041 tccgaaatta tcgaaggtat tacctcaatg gccaaatcga gtggactgct cgacgaggca
     2101 tttactcaaa tgacggtaaa catagaaagc gtgcgccaaa ccttctcggg attctctgag
     2161 ggtttacgta cctcccgatc cgatacccaa ggggttttaa cgactatgaa taccctccag
     2221 gaggtatcct ccgatgtaac gcgcgattcg gctcttgtgt ccgatggagc cggggatatt
     2281 gcttcttcga tgaacgagct tgatatgctc tccagtcggg tctttgacgg tattactgcg
     2341 atgtctttga tgctcgatgg gcttaaagag gttatgactc agtttagaac atacgcagac
     2401 ggtataattg cttcgggaaa taatcttgct tctcatatac ctcagttgaa gtagtctttt
     2461 ttttatgata ccttccgtgt catatgaaac tcgataaaac acttaaggac cttgccgcgg
     2521 cctttcccgc ggactttacc gaagctgaaa aagcccaggc aaaaaccatt ttcttaaaga
     2581 aatt
//
LOCUS       DAOQXQ010000201         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_44336_length_2573_cov_3.006354, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000201 DAOQXQ010000000
VERSION     DAOQXQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44336_length_2573_cov_3.006354"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKH81_04160"
     CDS             complement(<1..825)
                     /locus_tag="PKH81_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97262.1"
                     /translation="MQYLFAILQLIVLAIVFISLIVAALSILQTKMLLRRNAKKIRSM
                     GISRTTLSNEEAEAAKRIFPRYAKRIVAGDTVTSIAGVYGYMSIQSKYGSETRTNQTI
                     GGLPFLYLQSMETALLDNEENTATLLILQDGTVLPISINDSWTVKKEADIQNHITTNT
                     IGETSSDGWKISQRREPVTLSDYQFYHPRVNVLPSILLFIGSILLLVWDQPTSLYVLY
                     TGSIVTVIALIGIFYPERSIKANIPLLTLSGIAVRKHDMLLIDRFQILLSSLASKKI"
     gene            953..>2573
                     /locus_tag="PKH81_04165"
     CDS             953..>2573
                     /locus_tag="PKH81_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ97263.1"
                     /translation="MTHTDASNPAIRNIAIIAHVDHGKTTLVDAMFGQSGTFREGKDI
                     PERIMDSMDLERERGITIAAKNCSVMWKGIKINILDTPGHADFGGEVERALSMVDGVV
                     LLVDASEGPLPQTRFVLSKALALGHAVIVVINKIDRPDARASGVLSDIFDLFIDLGAS
                     DEQMEFPLLYAIGKKGIARPCLEGDMDSGTLEPLFDTILAAIPPPSFLSGDPFQMLVS
                     DLSYSDYFGRQAIGKVINGRAHSKDSLVCIGENDKRKNLNVTKLQTYDGITLVEAQSA
                     ESGDIIVLSGIEDVTIGDTICTRDAPKALPRIVIDEPTVSMRFSVNTSPIAGTEGKNP
                     TSTVLLERLKKETLKNVSLQVDEENIGGSYLVRGRGEFQLVILIEMLRREGLEIGVTR
                     PEVLFKYKDGKKLEPIEHVYIDCAEGYAGIVTMKLTGHKGRMLTYTSHEGARVHLEFS
                     VPSRALIGYRDQFLTDTKGTGIMNTILSGYEEYRGDFDDFTNGFLVSDQAGKGVTYGL
                     YHLEPHGQLFITPGEPIYEGMIIGQHNLAYNL"
BASE COUNT          720 a          494 c          630 g          729 t
ORIGIN      
        1 aatttttttg ctggcaaggg aagaaagaag tatctggaac cggtctatca ggagcatgtc
       61 gtgtttacgg acagcgatac cagaaagagt cagtaatggt atgtttgcct ttatagatcg
      121 ttcagggtag aaaatcccga ttagagcaat aaccgttact atagagcctg tgtacagaac
      181 gtaaagagaa gttggttgat cccatacaag taacagtatg cttccaataa aaagtaaaat
      241 ggaaggtagt acgtttacac gaggatgata aaattgataa tccgacagcg taaccggttc
      301 gcgtcgctgg gatattttcc atccatcaga ggatgtttcg ccaatggtgt tagtagtaat
      361 gtgattttgt atgtctgctt cttttttaac agtccaactg tcgtttatgg aaataggcaa
      421 gacggttcca tcctgaagaa tgagtaaggt tgcggtgttt tcttcgttgt cgagcagggc
      481 ggtttccatt gattgaaggt agagaaaagg aagtccgcca atggtttggt ttgtgcgcgt
      541 ttcgcttcca tatttacttt ggatgctcat gtatccgtat acgccagcta tactcgtaac
      601 cgtatctccg gcaactatac gttttgcata acgtggaaat attcgtttcg cagcttctgc
      661 ttcttcgttt gaaagtgtag tgcgcgagat gcccatgctt cgtatttttt ttgcgtttct
      721 acgcaagagc atcttggtct gtagaattga aagggcggca acgataaggg atatgaacac
      781 aattgcaagc acaatcagtt gaaggatggc aaatagatac tgcataacgc ataatagtga
      841 aaaaatatga gagggacaag ctgtcccgtc ggttgtacac tataccaaaa tccttaaaaa
      901 cggtactcta gtatgtacat cccacctcgt aatagagaaa tcaggaatta tattgacgca
      961 tacagatgca tctaacccgg cgattcgcaa tatcgcaatt atcgcccatg tagaccatgg
     1021 taaaacaacg ttggttgacg ccatgttcgg tcagtcagga acttttcgcg aaggtaaaga
     1081 catacccgag cgcattatgg atagcatgga tcttgaacga gagcgtggta ttacaatagc
     1141 tgccaaaaat tgttcggtta tgtggaaggg gataaaaata aacatccttg acactccggg
     1201 acacgcagat tttggcggag aggttgagcg agcgctttct atggttgatg gggttgtgtt
     1261 gctagtagac gcatcggaag gccccctgcc gcaaacccgt tttgtattga gtaaggcgct
     1321 tgccctaggt catgcggtta tcgttgtaat aaataaaatt gatcgtccag acgctcgcgc
     1381 atcaggggta ttaagcgata tcttcgattt atttattgat ctgggcgcaa gcgatgagca
     1441 aatggagttt ccacttctgt atgcaattgg taaaaagggt attgcacggc cctgccttga
     1501 aggcgatatg gatagtggta ctcttgaacc tctgttcgat acgattcttg ctgccattcc
     1561 ccctccctca tttttaagtg gagatccatt tcagatgtta gtatccgatc tttcctactc
     1621 ggattatttt ggtcgccagg caatcggtaa ggtgattaac ggtcgcgctc attcaaagga
     1681 ctctcttgtc tgtattggag aaaacgataa gagaaaaaac ttgaatgtga ccaagctaca
     1741 aacctatgat gggattacgc ttgtagaggc tcaaagcgcg gaaagtggag acatcatcgt
     1801 tctttcgggt atagaggacg ttacaatcgg tgatactatt tgtacacgcg atgcgccaaa
     1861 ggcactgccc cgaatcgtta ttgatgaacc gaccgtatcg atgcgctttt ccgtaaatac
     1921 ctctcccatc gctggaacag aaggtaaaaa tcccacgtca actgttttgc ttgaacgact
     1981 taaaaaggaa actttaaaaa acgtgtcttt gcaggtggac gaggaaaata ttggtggcag
     2041 ttatctggtt cgcggacgcg gagaatttca gttagtcatt ttaatcgaga tgcttaggcg
     2101 tgaaggtctt gaaattggcg ttaccagacc agaagttctt tttaaatata aagatggaaa
     2161 aaagcttgag ccaattgaac atgtgtatat agattgtgcg gaaggatatg ccggtattgt
     2221 aacgatgaag ttaactgggc ataaggggcg tatgctcacg tatacaagtc acgaaggcgc
     2281 tcgtgtacat cttgagttct ctgtgccttc cagagccctt attggatacc gcgatcaatt
     2341 cttgaccgat acaaagggta cgggtattat gaacacgatt ctttcgggat acgaggaata
     2401 tcgaggagac tttgacgatt ttactaacgg gtttctagtt tccgatcaag caggaaaggg
     2461 tgttacctat ggattgtatc atcttgaacc ccatggtcag ctttttatta caccgggtga
     2521 acccatatat gaaggcatga taataggtca gcataacctt gcctataatc tag
//
LOCUS       DAOQXQ010000202         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_44484_length_2568_cov_2.729805, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000202 DAOQXQ010000000
VERSION     DAOQXQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44484_length_2568_cov_2.729805"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKH81_04170"
     CDS             complement(<1..744)
                     /locus_tag="PKH81_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNQ97264.1"
                     /translation="MIFPTFGFALFFLCVFVGFWYVFRRHRDRRLFLTAASYLFYAMW
                     DWRFCFLLLASTSVNYGFGLLLGSNKDYRWRKLFMILSACLNLLILGFFKYYNFFAEE
                     LNALFSLSRHGGEGFLFPVLSVIMPVGISFYTFKAMSYVFDVYLCKIPPCRSFLDLAL
                     YISFFPQVASGPIVHAVDFLPQIEGVCASGLEPGSKPIEFSRSSALLVSGLLKKLVFA
                     NFLSTLFVDPVLADPASFSAIENFLAAIGY"
     gene            complement(751..2451)
                     /gene="ilvB"
                     /locus_tag="PKH81_04175"
     CDS             complement(751..2451)
                     /gene="ilvB"
                     /locus_tag="PKH81_04175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNQ97265.1"
                     /translation="MKYSGARIVVECLAEQGVQTVFGYPGGTILNIYDELYKNSDRIT
                     HILTAHEQGACHAADGYARASGKVGVVMATSGPGCTNLVTGIATAYMDSVPLVAITCN
                     VPTALLGKDSFQEVDITGITMSITKHNYIVRDVKDLAGVVREAFDIARCGRPGPVLID
                     IPKDITAATCEYEPVVIATCEKTAMVERCVAKTPEPSDSEFERAIALLSGAKRPFFYV
                     GGGVVISEATAELAALAEKIASPVAVSLMGKTAFPAKHELCTGMIGMHGTKASNTAAN
                     QCDLLVAIGARFSDRVISDPSRFAKSSKILQIDIDPAEINKNIRTEACLNGNIRTILA
                     RLAKEVSPRARDEWNDKVDEWKTHIPSMYTRKTSLHPRFVLETVHAKVGDDAIISTEV
                     GQHQMWVAQFYPFAKPRTFLTSGGLGTMGYGTGAAMGAQTAFPDRHVIHIAGDGSFRM
                     NLNELATISHYNMPIVIIVANNGTLGMVRQWQKMFYDQRYSATTLDRPPDFVKLADAF
                     AIRGFRAHTEEEFRVAFDKAIGERTPALIDCYMDIDEMVFPMVPAGKPIDELLLDAQS
                     "
     gene            complement(2448..>2568)
                     /locus_tag="PKH81_04180"
     CDS             complement(2448..>2568)
                     /locus_tag="PKH81_04180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNQ97266.1"
                     /translation="EELALRKAQWKPIAPKVTTGYLARYAKLVSSADTGAVLS"
BASE COUNT          733 a          625 c          646 g          564 t
ORIGIN      
        1 gtatccaata gcagccagga aattctctat agcagaaaag gaagccggat ctgcaagaac
       61 cggatcgaca aacagggttg acagaaaatt ggcaaagaca agttttttaa gtagaccgga
      121 gaccagtaag gcacttgaac gggaaaattc aatcggcttg gatcctggct ccaaaccgga
      181 ggcacagacg ccttctatct gcggcaaaaa atcaacggca tgaacgatag gtcctgaagc
      241 gacttgcggg aaaaaagaaa tatagagggc aagatcaagg aaggagcgac aaggaggaat
      301 cttgcacaga tacacatcaa atacatagct cattgcctta aaggtataaa aggaaatacc
      361 aacgggcatg atcaccgaaa gaaccggaaa gagaaacccc tctcctccgt gccgcgagag
      421 tgaaaaaagt gcgttaagct cttctgcaaa gaaattgtaa tacttaaaaa aaccaagaat
      481 caggaggtta aggcaggccg acaatatcat aaaaagcttg cgccaccggt agtctttatt
      541 cgatccaagc aatagaccaa aaccgtagtt taccgatgta gaagcgagca gtaaaaagca
      601 aaaccgccag tcccacatcg catagaagag gtagctggcg gccgttaaaa aaagtcgtcg
      661 gtcccggtgc cgcctaaaga cgtaccagaa cccgacaaac acgcagagaa aaaaaagcgc
      721 aaaaccaaag gttggaaaaa tcatcttccg tcaggattgc gcatccagaa ggagttcgtc
      781 aataggcttt ccggccggaa ccatcggaaa aaccatctcg tctatatcca tatagcagtc
      841 gatgagcgca ggagttctct ctcctatcgc tttgtcgaag gcgacacgaa actcttcttc
      901 ggtgtgagcc ctaaacccgc ggatggcaaa agcgtcggcg agtttaacaa aatcaggagg
      961 tcgatccaaa gtagttgcag aataccgctg gtcgtaaaac atcttttgcc actgcctgac
     1021 cataccaagg gtgccattgt tggcaactat gataacgatg ggcatattgt agtggcttat
     1081 tgtcgcaagc tcattaagat tcatgcggaa cgatccgtcg ccagcgatat ggattacatg
     1141 acgatcggga aaagcggttt gggcacccat tgccgctcct gttccatagc ccattgttcc
     1201 caaaccgccc gaggtcaaga aggttctggg ctttgcgaag ggataaaact gggcaaccca
     1261 catctgatgt tgaccgacct ccgtactgat tattgcgtca tcgccaacct tggcatggac
     1321 tgtctcgagt acaaagcggg gatggaggga tgtcttgcgg gtatacatgg aaggaatgtg
     1381 ggttttccat tcatctacct tatcattcca ttcatcgcgt gcgcgaggag aaacctcttt
     1441 ggcaagacgg gcaagaatgg tgcgaatatt gccattaagg catgcttcag ttctgatgtt
     1501 cttgtttatc tcggcgggat ctatatctat ctgaagaatc ttgcttgatt tggcgaagcg
     1561 tgagggatct gaaataaccc gatcagaaaa acgcgctcca atagcaacca ataaatcgca
     1621 ctggtttgct gccgtgttgg aggccttggt cccgtgcata ccaatcatac cggtacacaa
     1681 ctcgtgcttg gcgggaaaag cggtttttcc catcaggctc accgcaaccg gagaagcaat
     1741 tttttccgca agggcagcca gttcggccgt agcttcggaa ataacgacgc cccctccgac
     1801 ataaaagaag ggtcgcttcg cgccggaaag caaggcgatt gcgcgctcaa actccgaatc
     1861 ggacggctcg ggggtcttgg cgacgcaccg ttcaaccatg gcagtttttt cgcatgtagc
     1921 gatgacaacc ggctcatact cgcaggtagc ggcggtaata tccttaggta tgtcgataag
     1981 gacgggaccg ggtcttccac agcgggcgat gtcaaaagct tctcgaacca cacccgccaa
     2041 gtcctttaca tcgcgaacga tataattatg cttagtgata gacatggtaa taccggtaat
     2101 atcgacttcc tgaaaactgt ccttgcctaa tagcgccgta ggaacattgc aggtaatcgc
     2161 gacaagagga acagaatcca tatacgcggt ggcgataccc gttaccagat tggtgcagcc
     2221 gggaccgctg gtggccataa caacgccaac cttaccggaa gcgcgagcat agccgtccgc
     2281 tgcgtgacag gcaccctgct cgtgtgcagt gagtatgtgt gtaatacgat ctgagttttt
     2341 gtataactca tcgtaaatat ttaagatggt tccacccgga tagccaaaca ccgtctgtac
     2401 accttgctct gcaagacatt ctactacaat acgggctcct gaatacttca tgataatacc
     2461 gctcctgtgt cggcacttga taccaacttg gcgtatcgtg ctaaataacc ggtggttacc
     2521 ttgggggcaa tgggtttcca ctgagccttg cggagggcga gctcttca
//
LOCUS       DAOQXQ010000203         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_44513_length_2567_cov_2.936306, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000203 DAOQXQ010000000
VERSION     DAOQXQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44513_length_2567_cov_2.936306"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKH81_04185"
     CDS             <1..261
                     /locus_tag="PKH81_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNQ97267.1"
                     /translation="GLPQRGQDRLGNEWAVADPAPVVIFDAFADSGINILFGAWAETG
                     SYLDLKNSLMIEVYEAFAREGISIPFPQMDIHLDSTSQQLPG"
     gene            complement(239..634)
                     /locus_tag="PKH81_04190"
     CDS             complement(239..634)
                     /locus_tag="PKH81_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97268.1"
                     /translation="MSTILIVEDDESISDLMKTVLDGEGFRVITSPSAEGAKTLLQTE
                     ACDLAIIDIILPGQGGMDLILELHALCPNLAVILTSGKIDMNKATFKVLAHQFGVVSI
                     LPKPFTIEELLTSVHESLSGAAVNPGVVD"
     gene            complement(657..866)
                     /locus_tag="PKH81_04195"
     CDS             complement(657..866)
                     /locus_tag="PKH81_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNQ97269.1"
                     /translation="MSDQGEQRIIGLETKMAYLDDFMAKIQAITVEHTDAIERLKAEN
                     RALRAKLGEVEDQLQDLPNQRPPHY"
     gene            complement(974..>2567)
                     /locus_tag="PKH81_04200"
     CDS             complement(974..>2567)
                     /locus_tag="PKH81_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97270.1"
                     /translation="YEPSESLSRVFIIRNTGGNAISIVSITSDDSSAVSVDKPLLQAV
                     KLASQEELSFSLSFNPSNAVSATINIITDAPDSPSFSFKVYSTGVVLPFSHAHATSYR
                     LSANGFSFGYSDGSATRQIIIKNEGSSDITLNQPGSTVSPSTEYSIVSFSAPTVAAGQ
                     TVALTLRYTPSSSTAWSVATCTLVDTYGRSYAIRLFGSGYPQPPDTGVTPALWLSADR
                     IGESDTIILDGTRKVSTWKDASGNGLSADAFNYNGDILRPSYNSTGINGNPTITFSGT
                     SLRDHLRVYPQNGAILTTGTGTTSFIVFKTSASVVNTRYIIQAQNSGGTEVFPRFSYA
                     AWYFDPSDGFYASSTGGDPRVQYRFELAGYSGHRRHPLDSPTNQVETNATYMVNMKYN
                     LAVTTPNPNIFLRINEDDTDTPIGYLHSVSTTATSGLVGAYGFPIGNGLGAIDPTAGS
                     RANDYANSLSANNALFFNRITPSSFTYNSSLYYLHIGNSSNSTATFQGDIAEIILFAE
                     PLSEDNTRLVNRYLMNKYGIAY"
BASE COUNT          691 a          567 c          667 g          642 t
ORIGIN      
        1 ggcctgccac agcgtggaca agatcgcttg ggcaatgaat gggcggttgc cgatcccgcg
       61 cctgtggtca tctttgacgc ctttgccgac tcaggcataa acattctttt tggggcatgg
      121 gcagaaacag gaagctatct tgatttgaaa aactcactaa tgatagaggt ctacgaggct
      181 tttgcgcggg aaggtatatc tatccccttc ccacaaatgg atattcatct ggactcgact
      241 agtcaacaac tcccgggtta accgctgcac ccgaaaggga ttcgtgtaca gaggtaagta
      301 gttcttctat agtgaacggc tttgggagaa tcgatacaac gccgaactgg tgagccaaga
      361 ccttaaaggt tgccttgttc atatcgattt ttcccgaggt caggattacc gcaaggtttg
      421 ggcacagggc atggagctca agaataagat ccatcccacc ttgtcctgga agaataatgt
      481 cgataatagc aagatcgcag gcttccgttt gaagaagggt ttttgcgccc tctgcggaag
      541 ggctggttat aacacgaaat ccctcgccgt caaggacggt tttcatcaag tcggaaatgg
      601 actcatcatc ttctacaatc aatatagtac tcatagggta ttctccagtg taaggatcaa
      661 taatgtggtg gccgctgatt tggcaagtcc tgaagctgat cctcaacctc tccaagcttt
      721 gcccgtaagg ccctgttctc ggcttttaac cgttctatcg catctgtgtg ttctaccgtt
      781 attgcctgta tcttagccat aaaatcatcc agataggcca ttttggtttc taggcctatt
      841 atccgttgct caccttgatc gctcatggca gtgagtatac cacaaaatgg agttttttgg
      901 gaattggtta caatattaca agataaaaga aagaaaagcg gctgtctggc acctgaacca
      961 gacagccctc tacttaatat gcgataccgt atttattcat gagatagcga ttaaccagtc
     1021 gggtattgtc ctctgaaagg ggttccgcaa aaaggataat ctcggcgata tctccctgaa
     1081 aggtggccgt tgaattggag ctattaccga tatgaaggta atacaagctt gagttatacg
     1141 taaaggaaga aggcgtaatt cgattaaaga aaagggcatt attcgcgctg agggaattcg
     1201 cgtaatcatt tgcccttgat cctgctgttg gatcaatcgc gcccagccca ttaccaatgg
     1261 ggaaaccgta tgctccaact agccctgagg tggctgtagt agagacagaa tgcagatatc
     1321 caatgggagt gtcagtgtca tcctcgttga ttcgcaagaa gatattggga ttaggggtag
     1381 ttaccgcaag attatacttc atgttgacca tatatgttgc gtttgtttca acctgattcg
     1441 taggtgaatc aagaggatgt cgtctgtgtc cgctataccc ggcaagctca aaacgatact
     1501 gaacgcgagg atctccaccg gtactggaag cataaaatcc gtcagatgga tcaaaatacc
     1561 aagcagcgta ggagaaacgg ggaaaaacct cggttccacc gctattttgc gcctgaatga
     1621 tgtatctggt gttcacgacg gacgcggatg tcttgaacac gataaagctg gtagttcctg
     1681 ttccagtggt taaaatcgcg ccgttttgcg ggtaaacacg taaatgatcc ctcagtgagg
     1741 tacccgagaa agttatcgtc gggttgccat taatacccgt ggaattatac gaagggcgaa
     1801 ggatatcccc gttgtaatta aaggcatctg ccgaaagccc gttccccgat gcgtctttcc
     1861 aggtggatac ctttcgggta ccgtcaagga taatggtgtc cgattccccg atacgatcgg
     1921 cgctgagcca cagagcggga gtaaccccgg tatcgggagg ttgaggatac ccggaaccaa
     1981 acaggcgaat agcataactt cttccataag tgtcgacgag cgtacaggtg gcgaccgacc
     2041 aagcagtaga actggaaggc gtgtatcgaa gggtcagcgc taccgtttga ccagccgcga
     2101 cagtcggggc actaaatgat acgattgaat actcggtgga aggacttacc gttgatcctg
     2161 gttgattaag agtaatgtca gagcttcctt catttttaat aattatctgt cgtgtggcgg
     2221 agccatcgga gtagccaaag ctaaaaccgt tcgcagagag ccggtaagat gtagcatgag
     2281 catgcgagaa cggcaacacc actcccgtac tgtatacctt aaaagaaaag gaggggctat
     2341 ccggtgcgtc ggtgatgata tttatcgtgg cagaaacggc gtttgagggg ttaaacgaga
     2401 gggaaaaaga aagctcttct tgcgaagcta gcttaacggc ttgtaaaagc ggtttgtcca
     2461 ccgaaaccgc agatgaatca tccgaggtta tagagacgat ggaaatagcg ttcccgccgg
     2521 tattgcgaat gataaagacc cgagagaggg attccgaggg ctcatac
//
LOCUS       DAOQXQ010000204         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_44625_length_2562_cov_3.638612, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000204 DAOQXQ010000000
VERSION     DAOQXQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44625_length_2562_cov_3.638612"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1588)
                     /locus_tag="PKH81_04205"
     CDS             complement(92..1588)
                     /locus_tag="PKH81_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNQ97271.1"
                     /translation="METKSQTQVDVGKIKKATRMGIPLSITTYTLPHEIELYIAEVLA
                     CFLAELNQQKLKDYLVYCINELTTNAKKANTKRIYFAEKKLDINDSIDYEEGMRTFKE
                     DTLSNIDHYLLLQKEAGLYVKVLMQVKGRNIILEIRNNSEMTKTEFKRVFDKIARSRQ
                     FASLEDAFSQVMDDSEGAGLGLVIMILMLKKVGLDDENYEVMVEGGETITRISMPMDM
                     ETQEHMTRLTDDIVSFIDTLPQFPENIARIEKLLNDPESKLSTIASHISNDVVLTTDL
                     LKLVNSAAFSLQKKCTSIIEAVKLVGIRGIKNLLYSIGTMHLLGSATDEQKRLWDHSY
                     KAGFYTYNLARNFLKNRVVTEDAYVCGLLHDLGKIVFSAVYPDLLDKIQALKVQRNIP
                     DKVYDALLAGINHPEIGAALADKWNFPPALVAAIRFHHAVSDAPEEYRNIAATVCLAD
                     LMIHYQEKSIEFYQIDPALLKQFAITSEAHLQKICESFQRGFMSEQSR"
     gene            complement(1678..>2562)
                     /locus_tag="PKH81_04210"
     CDS             complement(1678..>2562)
                     /locus_tag="PKH81_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97272.1"
                     /translation="RIIAHAARLTDYRTLLTTRFTKISDLIIETDYQARKAGQTTITG
                     ETVSHAIENRRYLQRLPEEKYAQTITSREMVIDVSGSSIGKVNGLAVLDRGYHAFGIP
                     VAVSAQASPGTKGVVNIERESGLSGEIYDKAHLIIQGLLRGKYALSASICFEQSYTEV
                     DGDSASCAEFFALISAIACLPLRQDIAVTGSLNQLGDVQPVGGITEKIEGFFDTCSLL
                     GLTGTQGVIIPRRNLPNVLPSDRLLTAVQDGLFHIWAIDTIDEGLFLLSGMSPVDFGK
                     TVAQNLLAFTEHLKDFYR"
BASE COUNT          636 a          560 c          635 g          731 t
ORIGIN      
        1 cctacggcag ggtgagcata cccatgacgg gtattaaggc ttcgttaaac gtgggcgtcg
       61 cctttggtat tttgatgcaa gcctggacga gttatcttga ttgctcggac ataaagcccc
      121 gctgaaagct ttcgcagatc ttttgcaaat gtgcttctga agtgatggca aactgcttaa
      181 gcagtgcagg gtcaatctga tagaactcta tagacttctc ctggtagtga atcatcaggt
      241 ctgcaaggca caccgtagcg gctatgttac ggtattcttc cggcgcatct gaaacagcgt
      301 gatgaaagcg aatagccgct acaagggctg gtggaaaatt ccatttatct gccagtgccg
      361 ccccgatttc ggggtggtta atacctgcaa gcagggcatc gtaaacctta tccggaatgt
      421 ttctctgaac ctttaaggcc tgaatcttgt ccaatagatc gggatacaca gcgctaaata
      481 caatcttgcc taaatcgtgc aagagtccgc acacataggc gtcttcggta acaacgcgat
      541 ttttaaggaa attacgggca aggttgtagg tataaaagcc tgctttgtac gaatgatccc
      601 acaagcgttt ttgctcatcg gtggcagaac ccaaaagatg catagtaccg atcgaatata
      661 aaaggttttt gatgccgcgg atgccaacga gcttaacggc ttcgattata ctggtgcact
      721 ttttttgcag ggaaaaggct gcggagttaa ccaatttaag caagtctgtc gttaatacta
      781 cgtcgttact gatgtgcgag gcgatggtcg aaagctttga ctcaggatca ttaaggagtt
      841 tttcgattct tgcaatgttt tccggaaact gcggaagggt atcgataaag gaaacaatat
      901 cgtcggttag acgagtcatg tgctcctggg tttccatgtc catgggcata gagatgcggg
      961 ttattgtttc tccgccttct accattactt catagttttc atcgtccagg ccaacttttt
     1021 taagcataag aatcatgatt accagaccaa gtcccgcacc ttctgagtcg tccataacct
     1081 gtgaaaaggc atcctccagg ctcgcgaatt gccgggatct ggctatcttg tcgaataccc
     1141 gcttaaactc tgtttttgtc atttccgagt tatttcggat ctccaggata atgtttcgac
     1201 ccttaacctg catgagcacc tttacataaa gcccggcttc tttttgcagt aacaggtagt
     1261 gatcgatatt agaaagggtg tcttccttaa aggttctcat gccctcttca tagtcaattg
     1321 aatcgtttat atcaagtttt ttttccgcga agtaaatgcg tttggtattt gcctttttgg
     1381 cgttagtggt taattcgtta atgcaataga caagatagtc tttaagtttt tgctggttca
     1441 gttctgccaa aaaacaagca agtacttctg ctatatagag ttctatctcg tgaggaagtg
     1501 tgtaggtagt tatggagaga gggattccca ttcgggtcgc ttttttaatc tttccgacat
     1561 ctacttgagt ctgacttttt gtttccatat atggccagtc tatcctaggg ttggggtttt
     1621 ttccagtggt aagttggaca aagcgaaaaa aactggctga atagcggtcg attaggccta
     1681 gcggtagaaa tccttaaggt gttcggtaaa ggctagcaga ttttgggcca ctgttttccc
     1741 aaagtccaca ggactcatgc cggacaggag gaacagtcct tcatctatgg tgtctattgc
     1801 ccaaatatgg aaaagtccat cctgcactgc agtaaggaga cggtcggacg gtaaaacatt
     1861 gggaagattc cttcggggaa tgataacgcc ctgtgttccg gttaaaccaa ggagcgaaca
     1921 ggtgtcaaaa aagccttcta ttttttcggt aatacctcca actggttgca cgtccccaag
     1981 ctgattcagg cttcccgtta ctgctatgtc ctgacggagg gggaggcagg cgatggcgga
     2041 aataagggca aaaaattctg cacaggaggc ggagtccccg tcaacctcgg tataggattg
     2101 ctcaaaacag atgcttgccg acagggcata tttgccacgt aatagaccct ggataatcag
     2161 atgtgccttg tcgtaaattt ctccggacaa gcccgattcc cgttcgatat taacaacgcc
     2221 ctttgttccc ggagacgcct gcgcagaaac ggcaacaggg ataccaaagg catgatagcc
     2281 tctgtccagc accgcaagac cgtttacttt tccgattgag ctaccgctta cgtctatcac
     2341 catttcccga gaggtaatgg tttgggcata tttttcctcg ggaaggcgct gcaggtatct
     2401 gcggttttct atcgcatggg atactgtttc gccggtaata gtcgtttgac cggcttttcg
     2461 ggcttgataa tcggtttcga taattaagtc ggatattttg gtaaagcggg tggtcagaag
     2521 ggtccggtag tcggtaagcc gcgccgcgtg ggcaatgatt ct
//
LOCUS       DAOQXQ010000205         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_45383_length_2535_cov_2.601210, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000205 DAOQXQ010000000
VERSION     DAOQXQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45383_length_2535_cov_2.601210"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKH81_04215"
     CDS             complement(<1..313)
                     /locus_tag="PKH81_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97273.1"
                     /translation="MRHVFSFSSPFYYRRYFTILTCVQHKERIVGTPVLYLFRSSELF
                     VTQYNTLPIDYPDIGEESLLDSMDIDIAGVPSRILLFKDEASRAAVVSASVCGPPVSS
                     DP"
     gene            295..798
                     /locus_tag="PKH81_04220"
     CDS             295..798
                     /locus_tag="PKH81_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNQ97274.1"
                     /translation="MKKHVAFLLFALVSIALYAADPVEGFWKSIDEETGKTTAAWRIY
                     EKDSVLYGEIVTVPNQDDATIAKDCKSSYKGFPVAGEVNKMTVVHTPFIFGLKKKSEG
                     QWSDGNIIDPKDGKMYKCKITLRKADGKKFTKEVLEMRGEIGLGIGRSQYWERIDETE
                     IASLRSN"
     gene            915..1397
                     /gene="purE"
                     /locus_tag="PKH81_04225"
     CDS             915..1397
                     /gene="purE"
                     /locus_tag="PKH81_04225"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNQ97275.1"
                     /translation="MKVAIFFGSQSDTAIMKKAADVLRDFGVEFSSYPISAHRAGKLL
                     TQTVEQFESEGGEVIIAGAGLAAHLPGVIASMTVLPVIGVPLDGGKVGGLDALLSIVQ
                     MPKQIPVATVGLDNAANAAYLACSILAIKYPDLKAKLVAFRAKLAQDIAENLGGGNVL
                     "
     gene            1427..2134
                     /locus_tag="PKH81_04230"
     CDS             1427..2134
                     /locus_tag="PKH81_04230"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNQ97276.1"
                     /translation="MVKGEQMYEGKAKKVFTCNYPDMVVIDYKDDATAFNGEKKGQIE
                     SKGVLNNSITSGLFGLLEAKGVKTHFIAKLSDREMLCRKVHIIPLEVIVRNVAAGSFS
                     KRLAVAEGTELKTTIFEVSYKDDSLGDPLINDYHAVAIGAATWKELKTIYKMTARINR
                     ILSAFFAARGIRLIDFKLEFGKTADGKIILADEISPDTCRLWDAKTNEKLDKDRFRRD
                     MGNVKEAYVEIFNRISK"
     gene            2153..>2535
                     /locus_tag="PKH81_04235"
     CDS             2153..>2535
                     /locus_tag="PKH81_04235"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757815.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNQ97277.1"
                     /translation="MNTHSECATETKLREKCGVIGIYLTDHEMNASRLAYYGLQSLQH
                     RGQESTGIAVSDGERLDQIRELGLVADVYNNDNLAALKGYIAIGYVLYSTSGKATIEN
                     AQPFVSRSKLGNVAIAHNGSLVNAEP"
BASE COUNT          730 a          611 c          617 g          577 t
ORIGIN      
        1 aaggatctga tgatacaggt ggtccgcaaa cagaggcaga tacgaccgca gcccggcttg
       61 cctcgtcctt gaataacagg attcgggacg gcacacccgc aatatcaata tccatggaat
      121 caagcaggga ttcctcgcca atatccggat agtcaatcgg caatgtgtta tactgtgtta
      181 caaataattc gctggacctg aaaaggtata aaaccggtgt tcccactata cgttccttgt
      241 gttgcacgca ggtgagtata gtaaaataac gccggtagta aaagggagaa gaaaatgaaa
      301 aaacatgtcg cattcctctt gttcgcgctg gtgagcattg cattatatgc agccgatccc
      361 gttgaaggct tttggaagag cattgatgaa gaaacaggca aaacaactgc tgcctggcgc
      421 atatatgaaa aggactctgt tctgtatggc gaaattgtaa cggtgcccaa tcaggacgac
      481 gctaccatag caaaagattg taagagtagc tacaaaggct ttcctgtcgc cggagaagta
      541 aacaagatga ctgttgtaca cacacccttt atctttggtc ttaagaaaaa gagcgaagga
      601 cagtggtcag acggtaacat catcgatccc aaggacggaa agatgtacaa gtgcaagatt
      661 acccttcgta aagcagatgg aaagaagttt accaaagaag tgcttgagat gcgcggggaa
      721 atcgggcttg gaattggccg aagtcagtat tgggagcgaa tcgacgaaac cgagattgca
      781 tccctacggt ccaactaggc atacacgacg cgaaaagcgt aaaaaagtac caacaatcct
      841 gcgagcagta ggtatgttca caaacatacc tctaccgtct tgaaacctcc tccccttcta
      901 tgctagattc gattatgaaa gtcgccattt ttttcggatc acaatcagat acagcaataa
      961 tgaagaaggc cgccgatgtc ctcagggact ttggcgttga attctcatcc taccccattt
     1021 cggctcatcg cgcgggcaaa ctgcttaccc agaccgttga gcaatttgaa agcgaaggtg
     1081 gagaagttat tatcgccggt gcaggccttg ccgcgcatct tcccggtgtg attgcctcta
     1141 tgaccgtctt acccgtaatc ggtgtacccc tggacggtgg caaggtcggc ggactcgacg
     1201 cacttctttc tatagtacaa atgccaaaac aaatacccgt cgcaaccgtt ggccttgata
     1261 acgcggcaaa tgccgcctat cttgcctgtt ccattctggc aatcaaatat cctgatctta
     1321 aagcgaagct ggtcgccttt cgggcgaaac tggcccaaga tatcgcggaa aatctcggag
     1381 ggggaaacgt attatgagca agaaaactga caacctggtg cgcaatctgg taaagggaga
     1441 gcagatgtat gaaggaaagg ctaaaaaagt ctttacatgc aactatcccg atatggtcgt
     1501 aatcgactac aaagatgatg cgaccgcctt taatggcgag aaaaaaggac aaatcgagtc
     1561 caagggcgtt ttgaataaca gcattacctc cggtcttttt ggactactcg aggccaaggg
     1621 agttaaaact cactttatcg ccaagctttc cgatcgcgag atgctttgcc gcaaggtaca
     1681 catcattccc cttgaagtta tcgtacgaaa tgttgccgca ggaagttttt ccaaacgcct
     1741 tgcggtagcc gaaggcaccg aacttaaaac aactattttt gaggtttcgt ataaagacga
     1801 ctcactcggt gatccactta tcaacgatta ccacgctgta gccattggcg cggccacctg
     1861 gaaagaacta aaaaccatct acaagatgac cgcacgcatc aatcgtattc ttagcgcgtt
     1921 tttcgccgca cgcggcatcc gtctgatcga ctttaaactg gaattcggca agaccgcaga
     1981 cggaaagatt atccttgccg acgagatttc gcctgacacc tgccggcttt gggacgcaaa
     2041 aacgaacgaa aagctcgaca aggaccgttt ccgccgcgat atgggaaacg ttaaggaagc
     2101 ctacgtcgag atattcaatc gcatttcaaa gtaaacaagt aacggggtta tcatgaatac
     2161 acattcggaa tgtgcgacag agacaaagct gcgggaaaag tgtggcgtta tcgggatata
     2221 cctgaccgat cacgaaatga atgcgtcccg gcttgcatat tatggacttc agtctctcca
     2281 acaccgggga caagaatcaa ccggtatcgc ggtatccgac ggggagcggc tcgatcagat
     2341 ccgtgagctc ggcttggttg ccgatgtata caacaacgac aatcttgccg cgcttaaggg
     2401 ctacatcgcc atcggctatg tgttgtattc aacctcgggc aaggccacta tcgaaaacgc
     2461 gcaacccttt gtgtcgcgct ccaaactcgg taacgtggct atagctcata acggtagcct
     2521 cgttaatgcc gaacc
//
LOCUS       DAOQXQ010000206         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_45951_length_2515_cov_2.589837, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000206 DAOQXQ010000000
VERSION     DAOQXQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45951_length_2515_cov_2.589837"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKH81_04240"
     CDS             <1..196
                     /locus_tag="PKH81_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ97278.1"
                     /translation="KSNWELSTARAVNVLHYLTDFGADEKRFSVAGFADTRALYTNET
                     SEGRAYNRRVDIIILDEGHF"
     gene            230..775
                     /locus_tag="PKH81_04245"
     CDS             230..775
                     /locus_tag="PKH81_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNQ97279.1"
                     /translation="MSDQDDINLEGGDAGTGDSGGKKLGGLLPQLLKWVAIVLGALIF
                     IVTVVVITVNIMGSSGKSQTSIPVSEEYTGSREVMLWYREPLGTLQTRTSDTTPASVI
                     VEVALGYPVEDKATPAELSARLVELKDFLRAFFSRKTAAELRNEEKVKIEIRNAINDN
                     ILAKTKIRDVRFVKYEIVEQQ"
     gene            783..1817
                     /gene="fliM"
                     /locus_tag="PKH81_04250"
     CDS             783..1817
                     /gene="fliM"
                     /locus_tag="PKH81_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNQ97280.1"
                     /translation="MTEVLSQDEIDQLLTAISAGDTETEDFRPVNDTRKIKIYDFKRP
                     DKFSKEQIRTVSIMHETFARLTTTALSAQLRSMAHVHVASVDQLTYEEFIRSIPTPTT
                     LAVINMDPLKGNAILEIDPSVTFSIIDRLFGGTGQGTKVQRDLTDIETSVMEGIIVRI
                     LANMREAWTQVIDLRPRLGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIP
                     YITIEPIISKLSSQFWFSSVRRSSTTQYMGVLKEKLSTVDMDVVAEIGTINLPVRDVL
                     NLRVGDVVRLSNVRVGDPFTLSVGNKKKFLCQAGVIGKKVAVQVLKKVADLEQEDFEE
                     LTVEGDEAYE"
     gene            1810..>2515
                     /locus_tag="PKH81_04255"
     CDS             1810..>2515
                     /locus_tag="PKH81_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNQ97281.1"
                     /translation="MSDGSISQDEIDALLAGVDMGGMGAASAAPSSQFSDGQITALKG
                     FINANIPALKSNLDSMTGQSIDFGEPVIEVIQRDAFLRKLPEMIVATVIDFSEALSAD
                     HLFLLAPEFAQKLVSLVNHEPNAELDDMSLSVISETISQHVGIELTALEKAGFKGVAN
                     NPAESLHVPKAMVRFPQADFTLFTYPITIEGQAYSLWEVLTYQAGNDIANALGGGPAE
                     LPAAGMDALSSMGMGGM"
BASE COUNT          678 a          566 c          600 g          671 t
ORIGIN      
        1 gaagagtaat tgggagcttt ctaccgcgcg agcggtgaat gttcttcatt atctaactga
       61 ctttggcgca gatgaaaaac gtttctcagt agccggtttt gccgacactc gagccttgta
      121 tactaacgag acaagcgagg gtagggcata taaccgccga gtagacatta ttattctgga
      181 cgaaggacat ttttaatgtt actatatacc ctaaaaaagg aagcgcagta tgagtgatca
      241 ggacgacatc aatctggaag gcggcgatgc cggtaccggc gattccggag gaaaaaaact
      301 gggaggtctt ctcccgcaac ttctcaaatg ggtcgcgatc gtgcttggcg ctctcatttt
      361 tatcgttacc gtagtcgtaa taacggtaaa catcatgggt tcatcaggta aaagccaaac
      421 aagtataccc gtatccgagg aatacaccgg atctcgtgaa gttatgcttt ggtatcgtga
      481 acccctggga actcttcaaa caagaacatc agatacaaca cctgcttctg tcattgtgga
      541 agtggccctt gggtatccag tggaagataa ggcaaccccg gctgagttat ctgcccgcct
      601 tgttgaatta aaggatttct tgcgtgcttt tttcagtcga aagacagctg cggagctacg
      661 caacgaagaa aaagttaaaa tcgagatacg caatgcgata aacgacaata tattggcaaa
      721 aacgaagata cgtgatgtac gcttcgtcaa atacgagata gtcgaacagc aataaggaag
      781 ccatgacaga agttctttcg caggatgaaa tagatcaact tctcaccgcg attagcgcag
      841 gcgatacgga aaccgaggat tttcgcccgg taaacgatac tcgcaagata aagatctatg
      901 acttcaagcg acctgataaa ttctcaaaag aacagatacg tacggtttct atcatgcatg
      961 agacctttgc gcgtctaaca acaaccgccc tttccgctca attacggagc atggcccacg
     1021 ttcacgtcgc ttcggtagat cagcttacct acgaagagtt tatccgctct attccaacgc
     1081 ccacgacact tgcagttatc aatatggacc ctcttaaggg aaatgccatt ttggaaatcg
     1141 atccgtcggt taccttttct attattgatc gcctttttgg tggaaccggg cagggtacca
     1201 aggttcagcg ggaccttacc gatattgaaa cttccgtaat ggaaggcatt attgttagaa
     1261 tacttgctaa tatgcgcgag gcgtggactc aggttattga tcttcgcccc cgacttggtc
     1321 agattgaaac gaatccccag ttcgcccaga ttgttccccc ttcagaaatg gttgttttgg
     1381 taacccttga aacaaaggtt ggcgaggaag agggtatgat gaacttttgc ataccctaca
     1441 ttacaatcga accaattatt tccaagctgt ccagccagtt ttggttttct tctgttcgca
     1501 gaagttctac cacccaatac atgggtgtct taaaagaaaa actttctact gtagatatgg
     1561 acgttgtcgc agagatcggg acgataaatc ttcctgtgcg ggacgttctg aacttacggg
     1621 taggcgatgt tgtacgcttg tccaatgtac gcgtaggaga tccgtttacg ttgagcgttg
     1681 gtaacaaaaa gaaattttta tgccaggctg gcgtaatcgg taaaaaggta gcagttcagg
     1741 ttctaaagaa agttgccgat cttgaacagg aagactttga agaactcacc gtagaaggag
     1801 atgaagcata tgagtgacgg ttccatatcc caggatgaga ttgatgccct gttagctggt
     1861 gtcgacatgg gaggaatggg cgccgcatct gctgctccct cttcccagtt ttcggacggt
     1921 caaatcaccg cccttaaagg ttttattaac gcgaatattc ccgctctcaa gagcaatctt
     1981 gattccatga ctggtcagtc catcgatttt ggcgaacctg ttatcgaggt tattcaacga
     2041 gatgcttttt tacgcaaact gcccgagatg atcgtggcga ctgttatcga ttttagtgaa
     2101 gctctgagcg ctgatcatct atttttatta gctcccgaat ttgctcaaaa gctcgtaagc
     2161 ctcgtcaatc atgaaccgaa cgcagagctc gacgatatgt ctttatcggt tattagcgaa
     2221 actatctcgc agcatgttgg catagaactt accgcgcttg aaaaggccgg ttttaagggt
     2281 gttgccaata accctgcaga atcattacat gtgccaaagg cgatggttcg cttccctcag
     2341 gcagatttta ccctctttac gtatcccatt accatagaag gtcaagctta ctcgctatgg
     2401 gaggttctta cctatcaggc cggaaatgat attgccaatg cccttggtgg tggaccggct
     2461 gaactacctg cggcaggaat ggatgccctt tcctctatgg gcatgggtgg catgc
//
LOCUS       DAOQXQ010000207         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46062_length_2511_cov_2.451140, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000207 DAOQXQ010000000
VERSION     DAOQXQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46062_length_2511_cov_2.451140"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKH81_04260"
     CDS             complement(<1..1024)
                     /locus_tag="PKH81_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ97282.1"
                     /translation="MKRYVIVGGVAGGAGVAARLRRLDEQAEITMYERGEHISFANCG
                     LPYYAGGVITERSRLFVMTPSKFKESLNVEAKTLHEVVSIDREAKTVRVKNLTDGTYF
                     EQSYDTLILSPGATPIRPPIPGIEDNAILSLRSVSDIDCIKAKIDEAHTKRAVVVGGG
                     FIGIEMAENLKERGLEVTVVEAMDQVMNIIDYDMAAIVQGHLREKGVTLRLKNGVSSF
                     TRRGSFIDVTLADGQVIETDLVILSIGVKPDTAIAREAGLDLASNGAIKVDPYFNTSD
                     PSIRAVGDAIEFQSPLLGSMITIPLAGPANKQARLCAYNAVHGDSRPWKGTIATSIAR
                     VFDITVA"
     gene            complement(1106..1885)
                     /locus_tag="PKH81_04265"
     CDS             complement(1106..1885)
                     /locus_tag="PKH81_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNQ97283.1"
                     /translation="MADIYIGTSGYDYPEWKGVLYPHDLGREGFLAFYAEHFTALEIN
                     YTYYGMPNEYQMDAIARKSNGNLRFSVKAHKSLTHEVSISQWRKSAEEYRQALRPLVE
                     RNLLLSVLLQFPQSFHYEVDERRYLDALVNSLSPLPLVVEFRHASWQTKRVFDSLRER
                     GISWCVTDMPQLKHLPARLSIQTAASAYIRLHGRNAESWYGTNSRDRYDYLYSDTQLS
                     DLVPLVHRIAASSRIVQIYFNNHAKGQAVVNARKLKLLLNQ"
     gene            complement(1888..>2511)
                     /locus_tag="PKH81_04270"
     CDS             complement(1888..>2511)
                     /locus_tag="PKH81_04270"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HNQ97284.1"
                     /translation="GTALVFRERWRELNGIAAMDRQEQDANKNICSSKSFARPVLTLA
                     ELEEALASYTNEAVRKLREQSSAVRCISVFLMTNPWDDSGTPYYNQCAAALPESTSYL
                     PDILETAISLLHKIYRSGHRYRKIMINLLDLTDDTQAQAELFPTGDSRKEDKQALMRC
                     FDQINSRYGRTMLRTGSHTTAERTWAMRRQFLSPAYTTDLAGIPEVH"
BASE COUNT          589 a          595 c          664 g          663 t
ORIGIN      
        1 aggccacggt aatatcaaag actcgtgcga tgctggtcgc gatagtgccc ttccaggggc
       61 ggctatcacc gtgaaccgca ttgtaagcac aaagtcgggc ttgtttattt gccggtcccg
      121 caagcggtat ggtaatcata gaacccaaca aggggctttg aaattcaatg gcatctccta
      181 cggcacggat ggaagggtca gatgtattaa aatagggatc tacctttatt gctccgttgg
      241 aagcaagatc gagtcctgcc tcccgcgcta tagccgtatc gggttttaca ccgatagaca
      301 gaataacaag atcggtttcg attacctgtc catctgcaag ggtaacatca ataaaggagc
      361 cgcggcgggt aaaggaagat accccgtttt ttagcctgag ggtaacccct ttttcgcgaa
      421 gatgaccttg tacgatcgcg gccatgtcgt agtcaataat attcataacc tgatccatcg
      481 cttctacgac ggtaacctca aggcctctct cctttaggtt ttcggccatt tcaataccga
      541 taaacccacc gccaacgacg accgcacgtt tagtgtgcgc ctcgtcaatc ttggccttta
      601 tacagtctat atcgctaact gaacgcaggg agaggatggc gttgtcttct atgccgggta
      661 tggggggacg aatcggggtt gcgccgggac tgaggataag ggtgtcgtaa ctttgttcaa
      721 agtaggtgcc gtctgtaagg tttttaaccc gtaccgtctt tgcttcgcga tctatcgaca
      781 ctacttcgtg aagggttttt gcctctacgt ttaagctttc tttaaatttg gaaggagtca
      841 tgacaaacag tcgtgaccgc tcggtaataa ccccaccggc gtaatagggg agtccgcagt
      901 tggcaaagct tatatgttcg cctctttcgt acatggttat ttcagcttgt tcatcaaggc
      961 gtcgtaaccg ggctgcaaca ccagctccgc cggcgactcc acctactatt acatatcttt
     1021 tcatacctgc ccccttgtag ttcgtgtaca ggtttaattg tagtagaagg cgtaatttct
     1081 ttccgtgata aagttacctt agaaattatt ggttaagaag cagcttgagt tttcgggcgt
     1141 ttactaccgc ctgtcccttt gcgtggttat taaaataaat ttgaacaatc cgggacgagg
     1201 cagctatccg gtgtacgagc gggaccagat cgcttaattg ggtgtccgaa tacaggtaat
     1261 catagcgatc gcgagaattc gtcccatacc agctttctgc attgcgccca tggagacgaa
     1321 tataggcaga ggcagcggtt tggatggaaa gacgagcggg caggtgtttt aactgtggca
     1381 tgtcggtaac gcaccagctg attccacgct cgcgaaggga gtcaaagacg cgtttggttt
     1441 gccatgaagc atggcgaaat tcaaccacca ggggaagagg gctcagggag tttaccaggg
     1501 catccaaata tcgccgctca tctacttcgt aatgaaagct ttgcggaaac tgaagcagga
     1561 cagataaaag aaggtttcgt tcaacgagcg gtcgaagtgc ctgcctgtat tcttctgccg
     1621 attttctcca ttgggaaata gatacctcat gggtcaggct cttatgcgcc tttaccgaaa
     1681 aacggaggtt cccgtttgat ttacgcgcaa tagcgtccat ttggtactca ttgggcatac
     1741 cgtagtaggt gtaattgatc tccagggccg taaagtgttc ggcatagaag gccagaaatc
     1801 cttccctgcc aagatcgtgc ggatagagaa cccctttcca ttcaggataa tcatagcccg
     1861 aggttccgat atatatgtcg gccatgacta atgaacctcc ggaatcccgg caaggtcggt
     1921 ggtgtaggca ggagagagaa actgacgcct catggcccat gttcgctctg cggtggtgtg
     1981 actgccggtt cggagcatgg ttcggccgta tcggctgttg atttgatcaa aacagcgcat
     2041 aagcgcctgt ttgtcttcct ttcggctatc gcccgtggga aaaagttccg cttgtgcctg
     2101 tgtgtcatca gtaaggtcga gcaggtttat cattatcttg cgatagcgat gtccgcttcg
     2161 ataaattttg tgcaataggg aaatggctgt ttctaaaata tccggcaggt aggaagtgct
     2221 ttccggcagg gccgcagcgc attggttgta atagggggtg ccgctgtcat cccagggatt
     2281 ggtcataaga aatacggaga tgcatcgaac cgcgctcgat tgttctctga gttttctcac
     2341 tgcctcgttg gtatacgagg ccagggcttc ttccagctcg gcaagggtta gaacaggacg
     2401 cgcgaatgat tttgacgagc agatattctt gtttgcatcc tgctcttgcc gatccatggc
     2461 ggcaattcca ttgagttcac gccatcgctc gcggaacacg agcgcggtcc c
//
LOCUS       DAOQXQ010000208         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46239_length_2505_cov_2.477143, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000208 DAOQXQ010000000
VERSION     DAOQXQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46239_length_2505_cov_2.477143"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="PKH81_04275"
     CDS             complement(<1..1835)
                     /locus_tag="PKH81_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97285.1"
                     /translation="MKIKITSAVQKALDRSWADAKARSHEYVTPEHLLLAVLDSQSAI
                     RILSFCGADISFIHESVDEYIRTTIPVSPNRDPLQTVGFQSVLQRAVFHCENAEKSDL
                     EISDILVSLIDEERNHCSYFLRRGGLDRLALLEVISHAGIADDGEPHPEEGDPFVGGM
                     TEDPFLGKDPFDLRSIEEGRSADPQSSAGFSKKARGSGEEDKPSDGGDGETSRGQKSV
                     KKSFLERFAVELTALAKQGSLEPLIGRTEELERTVQVLCRRMKNNPVHVGDAGVGKTA
                     ITEGLAQLIARGEVPPFLRDFELYSLDMGALVAGTKFRGDFEERIKRVLDELLKKDKV
                     ILFIDEIHTMIGAGSTGGGTLDASNLMKPVLSSGKIRCIGSTTYEEYNKHFEKDHALS
                     RRFQKIDILEPSQEDTIKILEGVKTRYEDFHGVRYTASALESAVRLSAQYINERRLPD
                     KAIDVIDEAGARARILAGSLAPTDANTLEQGETLPVIDTFLIETVVARMARIPEKTVS
                     SGEVERLRDLEGVLHHAIFGQDIAVKAVSLAVKRSRAGFRAKGRPVANFLFVGPTGVG
                     KTELARVLADTLGVSLIRFDMSEYQEKHTVSRLIGSPPGYVGFDEG"
     gene            complement(1832..2137)
                     /locus_tag="PKH81_04280"
     CDS             complement(1832..2137)
                     /locus_tag="PKH81_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNQ97286.1"
                     /translation="MEELGKPQAFGSVSEETSEPAQYRVVLLNDDFTTKDFVVAVLVA
                     VFHKKQEEATVIMEDVHKKGRGVVGAYSYDIAATRCLQVHEAARQSGFPLRCVMEQM"
     gene            2224..>2505
                     /locus_tag="PKH81_04285"
     CDS             2224..>2505
                     /locus_tag="PKH81_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ97287.1"
                     /translation="MKTVVSWNVNGIRAVEKKGFLDWLSTESPDVLCLQETKAHKGQL
                     NPALVTPILPGGTWQSWWSSAKKAGYSGVAIYSKTEPLEVRTLGVDEFDD"
BASE COUNT          653 a          690 c          528 g          634 t
ORIGIN      
        1 ccctcatcaa aaccaacata tccgggggga gagccgataa gcctgcttac agtgtgcttt
       61 tcctggtatt cgctcatgtc aaaccggata agggagaccc caagcgtgtc cgctaacaca
      121 cgcgcaagtt cggttttacc cactcccgtt gggccgacga acaggaagtt agcaaccggc
      181 ctgccctttg cgcgaaatcc ggcacgggac ctctttaccg caagagacac tgccttaacc
      241 gcgatgtcct gaccaaaaat agcgtgatgc aagacccctt ccaggtcgcg taaacgttcc
      301 acctctcctg atgatactgt tttctccggt atcctcgcca ttcgtgcaac aaccgtttca
      361 atcaaaaagg tatctattac cggaagggtt tcaccctgtt ccagggtatt cgcatctgtg
      421 ggagcaagag aaccggcaag aatacgcgca cgtgcgcccg cttcatcgat aacatctata
      481 gccttatcgg gtagacgccg ctcgtttata tactgagccg aaagacgaac ggcactttca
      541 agagccgagg cagtatacct gacgccatga aagtcttcat aacgagtttt aaccccttca
      601 agtattttta tagtatcttc ctgcgacggc tcaagaatat ctatcttttg gaaccttcgc
      661 gaaagcgcat gatccttttc aaagtgttta ttatactcct cgtatgttgt agatcctatg
      721 caacgaatct ttcccgatga aagaacgggt ttcattaaat tagaagcgtc aagggttcca
      781 ccaccggttg agccggctcc aatcatggtg tgaatctcgt cgataaataa aataaccttg
      841 tctttcttta agagctcgtc aagaacccgc ttaatgcgct cttcaaaatc tccgcgaaac
      901 tttgttcccg caacaagagc gcccatatca aggctataca gttcaaaatc tcgcaagaat
      961 gggggaactt cccctcgggc gattaactgg gccaaacctt cggtaatggc cgtctttcca
     1021 acacccgcat cacccacatg aaccggatta ttcttcatac ggcgacagag aacctggacc
     1081 gttcgctcca actcttctgt tcgtccaatc aatggttcga gcgatccttg cttggctaaa
     1141 gcggttaatt ccaccgcaaa tcgttcaagg aaacttttct ttacgctctt ttgaccgcgc
     1201 gaagtctccc catcaccacc atcagatggc ttatcttcct cgccgctccc tctcgctttt
     1261 tttgaaaagc cggcagagct ttgcgggtca gcagaccttc cttcctcaat tgaacgaagg
     1321 tcaaacgggt ctttgcccaa aaagggatcc tcggtcatgc cgccaacaaa gggatcaccc
     1381 tcttccgggt gaggctcacc atcatctgcg attcccgcat gacttatcac ctccaaaagg
     1441 gcaagccgat caaggcctcc cctgcgaaga aagtaagaac aatgatttcg ctcttcatca
     1501 atcagactga caaggatatc agatatttca agatcagatt tctccgcgtt ttcacaatga
     1561 aataccgctc gctgtaacac actttggaag ccaactgttt gcagaggatc cctattgggc
     1621 gatacgggaa tcgtagtacg aatatactca tctacactct cgtgaataaa ggagatatcc
     1681 gctccacaaa aagaaagtat ccgtattgcc gactgggagt cgagcactgc caacaacaga
     1741 tgttctgggg taacatattc atgacttctc gcttttgcgt cagcccacga cctatccaga
     1801 gccttttgaa ctgcgctcgt tatctttatt ttcacatctg ctccataaca catctaagcg
     1861 gaaatccgct ttgccgggcc gcttcatgga cctgcaaaca acgagtggcg gcaatatcgt
     1921 aggaatatgc acctactaca ccccgtccct ttttatgcac gtcttccatt atgacggtag
     1981 cctcttcctg ctttttatga aacacagcca ctaataccgc taccacaaag tcctttgtgg
     2041 taaagtcatc attgaggagt actaccctgt attgcgccgg ctcgctcgtt tcttccgaaa
     2101 ctgaaccaaa agcctgcggc tttcccaatt cttccatact ccttaaaaaa tactgcaaaa
     2161 ccgctcagga aacaacagtt tccgctcttg aaagtacaga caggagatgg cattatagct
     2221 cgtatgaaaa cagttgtttc ctggaacgta aacggtatcc gcgcggtgga gaaaaaaggc
     2281 tttcttgatt ggctctctac tgaatccccc gacgtcctat gcctgcagga aactaaggcc
     2341 cataaaggtc agctaaaccc ggctcttgtt acccctatat tgcccggtgg aacctggcag
     2401 tcctggtgga gttccgcaaa aaaagcggga tattccggcg tggctatata cagtaaaaca
     2461 gaacctcttg aggtaaggac cctgggcgtt gatgaattcg atgat
//
LOCUS       DAOQXQ010000209         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46532_length_2494_cov_3.193112, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000209 DAOQXQ010000000
VERSION     DAOQXQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46532_length_2494_cov_3.193112"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKH81_04290"
     CDS             <1..894
                     /locus_tag="PKH81_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNQ97288.1"
                     /translation="SVRIPYENVVREALAPVARQLSITIIAFVLALTLSLAFARRLFV
                     YRIERLTEYTDALARGNLSVRSGVNNAQDEITELMESFNTMAASLEERNALNERTIRE
                     KEQLLLELQKRVSDNLQILSSIINLQIGYASNDEVRQSLMTTHSRVMALSLVYETLYL
                     YSDVQQVEMHRYSQGLCDYLVSLYTDVGTEIQCEIEGEEVALPIDKALPVALIINELV
                     SNSIVHAFPYRKNGFIGISFAKEDGRIRMEVRDDGSGFEEQEQRGETLGYEMIGALVE
                     QVHGSIIVNADENGSCVSVIF"
     gene            complement(901..1470)
                     /locus_tag="PKH81_04295"
     CDS             complement(901..1470)
                     /locus_tag="PKH81_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97289.1"
                     /translation="MWVYLEDVPGNQTTDDTPPIENLYSVARFVLSGMIYGWKFSYTP
                     SDRARSVAESFTLTPIMEIPSNDPNFVVESIKPAYPRLSCWAQYTLNQSDGRWVRYWA
                     SVLFKSAQGRGYGERTAETEGIRQAYTRAMLLAIRQNARSLEKNKPKEIHGEILLREG
                     PRLFADEGRFVAEVKVLIHISEIIPYRSF"
     gene            complement(1563..2198)
                     /locus_tag="PKH81_04300"
     CDS             complement(1563..2198)
                     /locus_tag="PKH81_04300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNQ97290.1"
                     /translation="MNSSSVLRKPFRYVYWNAAFVLIGINLLVFLLTAGSGKLAAYLS
                     LNPHLFLKNGFFWQVFTYQFVHGSMSHLLSNMLGLLFFGLTLEKKMGSKEFLLLYLLS
                     GTLCGIFSLFFYVIAGMWQIFLLGASGAVFAILLMYAVVFPRSIIYIWAILPVPAPLL
                     IMAYAAFETFYLVSGSRTGVAHSTHLIGFAVAWVYALVRFGINPARVWFGK"
     gene            complement(2243..>2494)
                     /locus_tag="PKH81_04305"
     CDS             complement(2243..>2494)
                     /locus_tag="PKH81_04305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ97291.1"
                     /translation="EEFCVMLCDTADKTAMKIAERIRKSIENLDILYEDQHLSLTISI
                     GVAQYNPEVDHSAKALVDRADKALYNSKQSGRNRVSLAE"
BASE COUNT          713 a          513 c          614 g          654 t
ORIGIN      
        1 tccgttcgaa tcccatatga aaatgttgtt cgggaagcgc tagctccggt ggctcgccag
       61 ctttcgatta caattattgc ctttgtcctt gcactaacgc tctctcttgc ctttgcacga
      121 aggttatttg tgtacaggat agaacggtta actgagtaca ccgatgcttt ggcaaggggt
      181 aacttgagtg taagaagtgg agttaacaac gctcaagacg agattactga actgatggaa
      241 tcatttaata ccatggcggc ttctttggaa gagcgaaatg cgctgaacga aagaacaatc
      301 cgcgaaaaag aacagttatt attggaactt caaaagcggg tatccgataa tcttcagatt
      361 ttatcaagta taatcaattt gcaaattggc tatgcctcga atgatgaggt tcggcaatca
      421 ctgatgacca cccattctcg agtgatggcc ctttctttag tctatgaaac cctgtatttg
      481 tactccgatg tgcaacaggt agaaatgcat cggtatagtc agggcttgtg tgactatttg
      541 gtgtctcttt atacagatgt cggaacagaa attcagtgcg aaattgaagg tgaagaagtt
      601 gcacttccta ttgataaggc cctgcccgtt gctcttatta ttaatgagct ggtctccaat
      661 agtattgttc atgcctttcc gtatcgtaag aatggtttta tcggaatttc tttcgccaag
      721 gaagatggac gcattcgaat ggaagttcgg gatgatggct cgggttttga ggagcaggaa
      781 caacgagggg aaactcttgg gtatgagatg ataggagccc tggtggaaca agttcacgga
      841 tctatcattg ttaatgccga cgaaaatggt tcctgcgttt cggtgatttt ttaagaatac
      901 ctaaaaactt cggtagggaa ttatctcgga aatatgtatt aaaaccttaa cctctgcgac
      961 aaagcgccct tcgtcggcaa aaaggcgtgg accttctcta agcaaaattt caccgtgtat
     1021 ttcttttggc ttgttctttt ccagggagcg ggcgttttgg cgaatcgcaa gaagcattgc
     1081 tctggtatat gcttgtctaa tcccttcggt ttcggccgtt ctctccccat atcctcggcc
     1141 ctgcgcgctt ttaaatagaa ccgaagccca gtatcgaacc catctaccat cactttgatt
     1201 taaggtgtat tgagcccaac aggaaagacg gggataggca ggttttatgg attccactac
     1261 aaaatttgga tcatttgagg ggatttccat tatgggtgta agtgtaaagc tctctgctac
     1321 tgagcgagca cggtcagaag gggtatacga aaatttccac ccatagatca taccggaaag
     1381 aacaaaacgg gctactgagt agaggttttc gattgggggt gtatcgtccg tggtctggtt
     1441 accgggaacg tcttcaaggt acacccacac tggcgcgcgg actattcgag gctcttgggc
     1501 aggcaaggtc gagcgaaagc taacaagagc gagaagacat gctgataaga aaatcctgtg
     1561 tgttatttgc cgaaccatac acgcgccgga ttaataccaa agcgtaccag agcatagacc
     1621 catgctacgg caaaaccgat aaggtgggta ctgtgtgcta ctccagttcg agaccctgaa
     1681 acaagataaa aggtttcgaa tgcggcgtag gccataatta aaagcggagc gggaacaggc
     1741 aagattgccc agatataaat gatggatcgg ggaaacacaa cggcgtacat gagcaggatt
     1801 gcaaaaacag cccccgatgc cccaagaagg aaaatttgcc acataccggc gataacataa
     1861 aaaaataaag aaaaaatgcc acaaagggtt ccggaaagga ggtacagaag gagaaattct
     1921 tttgatccca ttttcttttc cagggtcagc ccaaaaaaga gcaagccgag catattggaa
     1981 agaaggtggg acatactccc atgaacaaat tggtaggtga atacctgcca gaaaaagcca
     2041 ttttttaaga ataaatgcgg atttaacgat agatacgcag ccagttttcc acttccagcg
     2101 gtaagtagaa aaaccagcaa gttgataccg atgagcacaa aggctgcatt ccaatagacg
     2161 tatctgaagg gttttcttag tactgaagaa gaattcatgg gcttaacagt actccatgat
     2221 agcaatcggg gcaagaggct cgttattcag ccagagatac gcgatttcgt cctgattgtt
     2281 ttgaattgta cagggccttg tcggccctat ccacaagggc ttttgcggaa tgatccactt
     2341 cgggattgta ttgggcaact ccaatcgaaa tagtcaggct caggtgttga tcttcgtaaa
     2401 gaatgtccag attttctatg gatttgcgaa tacgttctgc aatcttcatg gcggttttat
     2461 cggctgtatc gcagagcatg acgcagaact cctc
//
LOCUS       DAOQXQ010000210         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46659_length_2490_cov_2.859548, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000210 DAOQXQ010000000
VERSION     DAOQXQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46659_length_2490_cov_2.859548"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..789
                     /locus_tag="PKH81_04310"
     CDS             139..789
                     /locus_tag="PKH81_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97292.1"
                     /translation="MKRSVLLSILFFSVVFSLAAQEKKDALKLFRNGRDLEAIGRTED
                     AKIAYSESIEVCKQDLVDNPRNMDAYTIYSWALFRLGKYQESVNICLEALKLVNDSRI
                     IESLAEGYFFLGNYKDSLKFMEKYIDAAPRGERLSTAHFFVGEIYRINKQYNRADIAY
                     TAAVHLEPGISLWWYRLATVRENVGDKSGAQQAYERALKLRPDYKDARDGLGRVRT"
     gene            complement(737..1825)
                     /locus_tag="PKH81_04315"
     CDS             complement(737..1825)
                     /locus_tag="PKH81_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNQ97293.1"
                     /translation="MQLRPLSLRGGEREALLVSRLAALCLFLSTIEFLIPKPLPFLRL
                     GLANAPLILALNLVSPTGYLALCLIKTVGQAIISGTLFSYIFLFSAAGTASSAISMFM
                     LSRLFRHHISSIGISVAGASASNAAQLLLARFLIFGESVMYMAPPILFAGLISGGVLG
                     ALTGRYTDSSSWYKETLEGRWPPQHIDLLPPEVPQNRREEAIQRLKIPFSLLLAGFLL
                     FSDSLLIKSVTTLCAAILLCISGKKIRILPTITITSGILFFNLLLPFGRVLAEPFGLP
                     ITEGALYSGIKKALVLEGMIFLSRWLLVHLPRLPGTVGALMGQTLAFLAYLTEHRSTV
                     SRKHLVASIDALMYGHDQVRPEHPYNPA"
     gene            complement(1807..2358)
                     /locus_tag="PKH81_04320"
     CDS             complement(1807..2358)
                     /locus_tag="PKH81_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ97294.1"
                     /translation="MTITKDSVVEIEYTLTDSQNEVIDSSEGSGPLEYLHGHENIIPG
                     LEKELEGKSAGATFKTTVAPLEAYGERQEELVVQVNRSQFPSDVELTEGMQFEAGSPD
                     GSRVVTVTAIDGDSITVDANHPLAGETLHFDVKVVSVRAATEEEKKSGLQSSCGCGCG
                     DDCGDDCDCEDGGCGSGGCSCGH"
BASE COUNT          689 a          541 c          636 g          624 t
ORIGIN      
        1 aacaggattc ttcttcgatc ttttcccgcg aatttcgtta ttcaaatgaa ttgtacgaga
       61 aaacaaaaaa tgttggcaaa cccctcctct tttcataaga tggttgcgaa aatcgttaaa
      121 aagggacata gtacagatat gaaacgaagt gtactgctgt cgattttatt tttttccgtt
      181 gtattctctc ttgctgcaca ggaaaaaaag gacgcgctca agctttttcg taatggccgg
      241 gatcttgagg ctataggacg gacggaagac gccaagattg cgtattctga atcaatcgag
      301 gtatgtaaac aggaccttgt ggacaatccc cgaaacatgg atgcgtatac tatttatagc
      361 tgggctttgt ttcggcttgg gaagtatcag gaatccgtca atatctgcct tgaggccctt
      421 aagttggtta atgactccag aattattgaa tcgcttgccg aagggtattt tttcctgggg
      481 aattacaagg attctttaaa gtttatggaa aaatatattg atgcggcgcc tcgtggagag
      541 cgattaagca ccgcgcattt ctttgtaggc gaaatttatc gaatcaacaa gcaatataac
      601 cgggccgata ttgcatatac ggcagcagtt catcttgagc cgggaatttc tttgtggtgg
      661 tatcgcttgg caactgttcg cgagaatgtt ggcgataaaa gtggagctca acaagcctat
      721 gagcgagcct taaagctcag gccggattat aaggatgctc gggacggact tggtcgtgtc
      781 cgtacataag agcgtcaatt gatgctacca ggtgtttccg gctgactgtt gaccgatgct
      841 cagtaagata ggcgagaaac gcaagggttt gacccatcag cgcacctacc gtgccgggga
      901 gtcggggcag gtgtactaaa agccaacgag aaagaaaaat catgccctcg agtacgaggg
      961 ctttttttat tcctgaatac aaggctcctt ctgtaattgg caagccaaat ggttcagcca
     1021 agacgcgacc aaagggaagt aagagattaa agaatagtat tcccgaggtt atcgtaattg
     1081 tgggcaggat gcggatcttt ttaccagaaa tacaaagaag aatcgctgcg cacagtgtgg
     1141 tcactgattt aatgagtaaa ctgtctgaaa aaagtaaaaa acccgcaaga agcaaggaaa
     1201 aagggatttt tagtcgttga attgcctctt ctcggcgatt ctggggaacc tctggcggta
     1261 gaagatcgat gtgctgcggg ggccaccttc cttccagggt ttccttatac catgaagagg
     1321 aatcggtata tcttccagtt aacgcgccaa gcacaccgcc ggaaataagt cccgcgaaca
     1381 atatgggagg ggccatatac attaccgatt ctccaaatat caagaagcgg gccagaagaa
     1441 gctgtgccgc atttgaagcg ctcgcccctg ctacgctaat gccaatgcta ctgatgtgat
     1501 gacggaataa acggctaagc ataaacatac tgatggcgga agaagcggta cctgctgcag
     1561 aaaaaagaaa aatataggag aagagcgttc cgcttataat ggcctgacct acggttttga
     1621 ttagacagag ggccagatat cctgttgggc taacaagatt tagggcaaga ataagcggag
     1681 cattggccaa gccaaggcgt aaaaagggaa gtggttttgg tatcaggaat tcaattgttg
     1741 aaaggaaaag gcagagggcc gcaaggcggg aaacgaggag cgcctcgcgc tccccgcccc
     1801 taaggcttag tggccgcagc tgcatccgcc ggaaccgcat ccaccgtctt cacagtcgca
     1861 atcgtcgcca cagtcatccc cacagccgca gccgcatgaa ctctgtagac cagatttttt
     1921 ttcttcttcg gtagctgcac gaacggaaac aaccttaacg tcaaagtgaa gggtctcgcc
     1981 agctaaagga tgatttgcat caacggtaat cgaatcacca tcgatggccg taacagttac
     2041 gacgcgggag ccgtcggggc ttcctgcttc gaactgcatt ccttcggtta attcaacatc
     2101 gctggggaac tgggatcggt ttacttgaac caccagttct tcctgcctct ctccgtatgc
     2161 ttccagaggt gctacagtcg ttttgaatgt agctccggca gacttgcctt cgagctcctt
     2221 ttcaagtccg gggatgatgt tttcgtgtcc gtggagatat tcaagaggtc cggaaccttc
     2281 agatgaatcg ataacttcgt tttggctgtc ggtgagggtg tattcaatct caacgacgct
     2341 gtctttggta atggtcataa aaagtccttc cattaagata ccccgaaaat aacacaatca
     2401 aggcctcggg gcaactggtt tcggctgtag acgtgaaata actatttctg atggacaaag
     2461 ctacttttgg gggctatact aaaaaaaagg
//
LOCUS       DAOQXQ010000211         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46870_length_2483_cov_2.470758, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000211 DAOQXQ010000000
VERSION     DAOQXQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46870_length_2483_cov_2.470758"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKH81_04325"
     CDS             <1..198
                     /locus_tag="PKH81_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNQ97295.1"
                     /translation="DGLDLVRSLIDHLPQVVKQGGKVLIETGEYNSQQASEYLNLKGF
                     TDIVIHADLSGQERVVEGSWK"
     gene            189..1976
                     /locus_tag="PKH81_04330"
     CDS             189..1976
                     /locus_tag="PKH81_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNQ97296.1"
                     /translation="MEITKLLDDFFADYPYLDGDDQALITQAWELMCQKRGEVRRLCG
                     EAFLEHPIRVARILASLQLDSPSIACAFLHGSMEELGFTADEIERQFGKTVRTLVEGT
                     SRISALKLKNKTIQQAESIRKMFFAMVDDIRVILVRLADRLDKMRSLKNFPIEQQRSI
                     AQETIDIWAPLANRLGISYIKDELEDLSLKYLNREVYEQIKNLVAAKKGERAEFLDSA
                     EKALYSASQQAGLSVEIHSRAKHFWSIYQKMKKRNKGVDELYDLLAMRILCSTVHECY
                     TLLGLVHTVWKPLDGRFKDYIAMPKPNGYQSLHTTVMCDHGRMLEIQIRTFEMHHIAE
                     NGIASHWLYKKGSGKEIVNADHLSIINQLKELSKGRFSDEDFLARIKTDLLGDSIYVF
                     TPKGDIVELPALSTAVDFAYHIHSAIGEKIVGARADGSIIPLSQALKNTQVIEIITHP
                     QAHPTQNQYNSVRTAKARQKIRAWLQANEPKDASEKKDDGEKQSPIQKQIHHRSTLPE
                     EQTRQFDSAVLKIRVGDTTNFMIKFAQCCEPMPPAPIIGYVSRGRGIIIHRTDCANLA
                     AIPDIQNRNIPVEWEIPVAIAQPTKKKKD"
     gene            complement(1973..>2483)
                     /locus_tag="PKH81_04335"
     CDS             complement(1973..>2483)
                     /locus_tag="PKH81_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ97297.1"
                     /translation="LVGIVTERDLINASPSEATTLSIFEISSLLASLKVEQVMTRKVI
                     SVDESVVIEEAARIMVDNGISALPVMKGDIPVGIVSDGDLFRLFINLFGARQNGLRIS
                     MLVPEKKGELHDLAEVLTQKGANVLSMVICDGCDVTNRMCIMKVSDISKEDLLDAVKP
                     CSLKVVDIR"
BASE COUNT          764 a          600 c          519 g          600 t
ORIGIN      
        1 gacggtcttg atcttgtccg ctcgttaata gatcaccttc ctcaggtggt aaaacaggga
       61 ggaaaggtcc ttattgaaac cggggaatac aactcacagc aagcgagcga gtatttgaat
      121 ctcaaaggat ttactgatat agtcattcat gcggatcttt ccggacaaga gagggttgtt
      181 gaaggatcat ggaaataaca aagcttcttg atgatttttt tgcagactac ccatacctcg
      241 atggcgatga ccaggccctt ataacgcaag catgggagct gatgtgccaa aaaaggggcg
      301 aggttcgtcg tctttgcggc gaagcattcc ttgaacaccc aatacgcgtt gctcgcattc
      361 ttgcctcact ccaactagac agtccatcca tagcctgcgc ttttttacat ggttcaatgg
      421 aagagctcgg gttcaccgca gacgagattg aacggcaatt cggtaaaacc gtacgcaccc
      481 ttgtagaagg tacctcgcga atttctgccc tcaagctaaa aaataaaacc attcaacaag
      541 ctgaatcaat cagaaaaatg ttttttgcca tggtcgacga tatccgggtc attctggtac
      601 gcctggccga ccgtctggac aagatgagaa gtcttaaaaa cttccccatc gagcaacaaa
      661 ggtccatagc ccaggaaaca atcgatattt gggcacctct ggcaaaccgc cttggcatat
      721 cctacatcaa agatgaactt gaagacctga gccttaaata ccttaatcgc gaggtatacg
      781 agcaaatcaa aaatcttgtt gccgctaaaa aaggggaaag agcggaattt cttgattctg
      841 cagaaaaggc gctctacagc gcatcccaac aagcaggtct ttctgttgaa atccactctc
      901 gcgcaaagca cttctggtca atttaccaaa aaatgaagaa acgcaacaaa ggcgtagacg
      961 aactgtacga tcttctggct atgcgtattc tctgttcaac ggttcatgaa tgctacaccc
     1021 ttcttggcct ggtccataca gtctggaagc ctcttgatgg aagatttaag gactatattg
     1081 ccatgccaaa acctaacggg tatcaaagcc ttcataccac tgttatgtgc gatcacggca
     1141 gaatgctcga aattcaaatc agaacctttg agatgcatca tatagcagag aacggtattg
     1201 ccagtcactg gctatataaa aaaggaagtg gaaaggaaat cgttaatgcc gatcaccttt
     1261 ccattatcaa tcagctaaaa gaattatcaa aaggcagatt ttccgatgag gatttcctgg
     1321 ccaggataaa gacggatctt cttggagatt caatctatgt ctttacccca aagggagata
     1381 ttgtcgaatt gcccgctctt tctactgccg ttgattttgc ataccacata cactcagcca
     1441 ttggagaaaa aatagtaggc gccagggcag atggatcgat tattcctctg tcgcaagcct
     1501 taaaaaatac gcaggtaatt gaaataatta cccatccaca ggctcacccc acgcagaatc
     1561 aatataattc agttcgaact gctaaagctc gccagaaaat tcgcgcctgg cttcaagcta
     1621 atgaacccaa ggatgcgagc gaaaagaaag acgatggcga aaaacaatct ccaattcaaa
     1681 agcagatcca tcatcgaagt acattacccg aagagcaaac tcgtcagttc gacagtgcag
     1741 tcctgaagat acgggttggg gacacgacta atttcatgat taaattcgct cagtgctgcg
     1801 aaccaatgcc ccccgcaccg ataattggat atgtgtcacg aggaagaggt ataattattc
     1861 atcgaacgga ttgcgcaaac cttgccgcaa taccggacat acaaaacaga aatataccgg
     1921 tggaatggga aatacctgtt gctattgccc aacccacgaa aaagaagaaa gactaacgaa
     1981 tatcgactac cttgagggag cagggtttaa ccgcatccaa aagatcttcc ttgctgatat
     2041 cgcttacctt cataatgcac atacggttcg ttacatcaca gccatcgcaa ataaccattg
     2101 aaagtacatt cgcgcctttt tgagttagga cctctgccag atcatggagc tcaccctttt
     2161 tctcgggaac caacatgctt atcctaagcc cgttttgtcg tgcgccaaaa agatttataa
     2221 acaggcgaaa caagtcaccg tcagatacaa taccaaccgg gatatcacct ttcataaccg
     2281 gcaaagcact aataccatta tctaccataa tgcgggcggc ttcctcgata actaccgact
     2341 catctacgct tataaccttc ctggtcatta cctgttcaac cttaaggctg gccaacagag
     2401 aactaatctc aaatatgctt agggtggtgg cctcagaagg acttgcgttt atcaagtccc
     2461 gctccgtaac gataccaaca agc
//
LOCUS       DAOQXQ010000212         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_46995_length_2479_cov_2.617574, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000212 DAOQXQ010000000
VERSION     DAOQXQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46995_length_2479_cov_2.617574"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKH81_04340"
     CDS             complement(<1..240)
                     /locus_tag="PKH81_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HNQ97298.1"
                     /translation="MSDTVMNDTFIEVGHYQLCKNGEAAPGDAFLSQKHDGDGRVITV
                     LSDGLGSGIKAGVLSTLTATMAMKFVASDIPIRRAA"
     gene            complement(237..2006)
                     /locus_tag="PKH81_04345"
     CDS             complement(237..2006)
                     /locus_tag="PKH81_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNQ97299.1"
                     /translation="MEFLSPIYTEKRQCQDCYKCIRECPVKAIRVENGHAMVVSGLCV
                     LCGHCVTICPAGAKRVRDDRSRARQLLSLKERVFVSLAPSFVSEFPEVSPANLVSAIK
                     KLGFFGVSETAIGADLVSSQLAEDFSSLKNRISASREKVGPHERLLLSSACPVAVEYI
                     KQYLPDLSPCVTDRASPLLAHARFLKDLYGPDIGVVFIGPCIAKKREADVWQTIDCAI
                     SFQDLRQWFAEEGVGLENTERKTSEGFVPFSAAKGSLYPIDGGMVSSIEKYPQAKGFR
                     TMAVAGLDEIDRALRDLHPEGLDDPLFIELLACPGGCLNGPLASREGSSAIRKLKLLE
                     YGGKAKDTATHRKQIDMQGVLPVERIIRPVHSDDEIRQALKQVGKLSLLDELNCGSCG
                     YDTCRQFVAAMLEHRAERTMCVSYMRKLAQKKANGLIRVIPSGVVICDADLKIIECNE
                     NFARLMGSETELMFEAKPGMEGADLALITGMSRFFTDVMTPTGPDVVEHEWREGKKIF
                     QATVFSIEKGESACGVIQDVTAPQVRRDRVIRQTRRVIDKNLAVVQKIAFLLGENAAE
                     TEATLNSIIESFSVDESDEEKTR"
     gene            complement(2010..2264)
                     /locus_tag="PKH81_04350"
     CDS             complement(2010..2264)
                     /locus_tag="PKH81_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ97300.1"
                     /translation="MDKPDVTITVCMGSSCFSRGNNLNVEIIKRFVDEYKLSASVEVV
                     GCLCDGHCKDGPNIRINDELIQGVLPAMVRDLLEHKLQRG"
BASE COUNT          695 a          647 c          494 g          643 t
ORIGIN      
        1 cgctgcacgg cgtatgggaa tatccgaagc aacaaacttc atcgccatgg tagcggtcag
       61 ggtagacagg acacctgcct taatgcccga acccaagcca tcagagagaa cagttattac
      121 ccgtccatcc ccatcatgct tctgggataa aaaggcatca ccaggggcag cctctccatt
      181 tttgcacagc tggtaatggc caacctcgat aaaggtgtcg ttcattacag tatcgctcaa
      241 cgggtttttt cctcgtcaga ctcatctacg gaaaacgact caataatcga gtttagagtc
      301 gcttcagttt ccgcagcatt ctcgccaagc aaaaaagcga ttttttgtac cacagccaag
      361 ttcttgtcta taacacgccg tgtttgtctg ataacccgat cgcgtcgaac ctgtggcgca
      421 gtaacatctt gaataacccc gcaggcactt tctccctttt cgatggaaaa cacggttgcc
      481 tggaatattt tttttccttc cctccattcg tgttctacca catctggtcc ggtaggagtc
      541 attacatcgg taaaaaatcg ggacatacca gttatcaagg caaggtcagc cccttccatt
      601 cctggcttgg cctcaaacat caactcagtt tcacttccca taaggcgggc aaaattttca
      661 ttgcattcaa taatctttag atccgcatcg caaattacta cgcccgaagg gataacacga
      721 ataagcccgt ttgccttttt ttgggcaagc ttacgcatat aggaaacaca catggtgcgc
      781 tccgcgcgat gttcaagcat cgctgccaca aattgtcggc atgtatcata tccacaactt
      841 ccacaattca attcatccag aaggcttaat tttccaacct gctttaacgc ttgcctgatt
      901 tcatcatcag aatgaacagg ccggataatg cgctctacgg gcagcacacc ctgcatatct
      961 atttgttttc gatgtgtagc ggtatccttg gcctttcccc catattccaa tagcttaagc
     1021 ttgcgaatcg cactagatcc ttcgcgagaa gctaacggac cattcaaaca gccgcccggg
     1081 caagccaata gttctataaa taaaggatca tcaagtccct caggatgcag atcccgaagg
     1141 gctctgtcta tctcgtcaag gcctgcgact gccatagtcc taaaaccctt tgcctgagga
     1201 tacttttcta ttgagctaac catccctccg tctattggat aaagggaacc ctttgccgca
     1261 ctaaagggaa caaacccctc actcgtcttt ctttcggtat tctctaatcc aaccccttct
     1321 tctgcaaacc attgacgcaa gtcctgaaag gaaattgcac aatctatcgt ttgccataca
     1381 tctgcttccc ttttttttgc gatacaggga cctataaaaa caacgcctat atcaggtcca
     1441 tacagatcct taaggaatct ggcatgagcc agtaagggtg aggcccgatc tgtaacacag
     1501 ggcgacaaat caggcaagta ttgcttgata tactcaaccg caaccggaca tgccgaggaa
     1561 aggagcagtc tctcgtgagg cccaaccttc tcccttgagg cggaaattcg gtttttaaga
     1621 gaagaaaagt cctctgcaag ttgtgaagaa actaaatcgg caccaatggc tgtttctgat
     1681 actccaaaaa agcccagttt ctttattgca gaaacaagat ttgccggact tacctccggg
     1741 aattcagaca caaacgaagg cgccaaagaa acaaatacac gctcttttaa ggacaataac
     1801 tgtcgagccc gagatcgatc atcgcgaacc ctctttgctc ctgctggaca gatagtaaca
     1861 cagtggccgc aaaggacaca taaacccgat acgaccatcg catgcccatt ttccacgcga
     1921 atagccttta cgggacattc tcgtatgcac ttgtaacagt cctggcactg tcttttttct
     1981 gtatagatcg gacttaaaaa ttccacgaat tacccccttt gaagcttatg ctccaaaaga
     2041 tcgcgaacca tggccggtaa aacaccctga atcaactcat cgttaatcct gatatttggc
     2101 ccgtccttgc agtgaccatc gcataaacaa cctacaacct caactgatgc cgataattta
     2161 tactcgtcaa caaaacgttt tataatttct acattgaggt tgttaccgcg ggaaaaacac
     2221 gaacttccca tacaaacggt aatagtaaca tccggtttgt ccatgagcgg cctccggggt
     2281 taatgtataa aaaaatatcg atttagtcaa tgagataaag gagatcacat gaacgaagga
     2341 ttggacagga acgctgggtt tacaacaaca gttgacagct tgcaggacct gtattcaagc
     2401 atccttgaat actacgatga gcttttcccg cttgatgagg gggcttgtga tttttttctt
     2461 ggtttaaaag aagaattac
//
LOCUS       DAOQXQ010000213         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47206_length_2472_cov_2.709971, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000213 DAOQXQ010000000
VERSION     DAOQXQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47206_length_2472_cov_2.709971"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKH81_04355"
     CDS             complement(<1..710)
                     /locus_tag="PKH81_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ97301.1"
                     /translation="MSRILVAMSGGVDSSVACTLLMDQGHEVSGCTLDLFDQSVSGSA
                     QPCPATKAIADSQKVAQRLGIEHRVLTIHGIFREQVIDNFIQTYLDGDTPNPCVQCNR
                     HIKFGALIDEAVRLGFDSIATGHYARVRKDERTGRYLLQKAIDPAKDQSYVLYSLQQD
                     QLARAVFPLGGMSKAEVRSIAAREGFESAHTGESQDICFLPDGDYAGFSERERPEAIT
                     EGNYLDVQGSIIGRHRGV"
     gene            complement(715..>1307)
                     /locus_tag="PKH81_04360"
     CDS             complement(715..>1307)
                     /locus_tag="PKH81_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ97302.1"
                     /translation="ATHHFLTKVRGIPARVTGLDLKKDVIDHCQALVQRYGSSGLRFL
                     TGDIEHYSCDTPPDVVISLHACDTATDAALAKAVSWGSKAILAVPCCQHELNARLAEK
                     DTSSPAEQELAYALKHGLVRERIAALLTDASRAAVLEQYGYRVQLLEFIDMSHTPKNI
                     LIRAIKKTQTHSIPTRACRPFGRELSLERLLGGDKE"
     assembly_gap    1308..1317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1318..1924)
                     /locus_tag="PKH81_04365"
     CDS             complement(<1318..1924)
                     /locus_tag="PKH81_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ97303.1"
                     /translation="MNLKIDNPSEFIARLAAGEGPLGPLVSLTLSAPKKGELPHGERV
                     RIRALPASDSAFQKETIRDNKAFQQNLSAPELSNFVIEVFPSLFKRAEAQCAQGTISF
                     LSNRKGSLTALIREEATCRQASAHNREKKYLLSEDDPVPFLVDLGVMTAEGNLIKAMT
                     PKFRQINRYLEFIDDIIDEVVAGLDENRVLEVVDFGCGKSYL"
     gene            complement(1914..2261)
                     /locus_tag="PKH81_04370"
     CDS             complement(1914..2261)
                     /locus_tag="PKH81_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97304.1"
                     /translation="MTLKTIIEELPAHLVWGPQGWQNIAITRVIAGDLMSDILVMVET
                     EALLVTGLATDQSVRCADMVGASAVLVVNDKLPDPSMRLHAEAAGIPVLATPMPLFET
                     CVTLNFLMDKHES"
     gene            complement(2258..>2472)
                     /locus_tag="PKH81_04375"
     CDS             complement(2258..>2472)
                     /locus_tag="PKH81_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNQ97305.1"
                     /translation="AMIGEDSIIGAMSLVTSGKSFPPRSLLMGSPAKLVRTLTDEEVE
                     KNRKNALHYRYLAASAMNDYQDAETV"
BASE COUNT          547 a          626 c          669 g          620 t
ORIGIN      
        1 cttacgcccc ggtgacgtcc aataatagag ccctgtacat caaggtagtt tccttcggta
       61 attgcctcgg ggcgttcccg ctccgaaaat ccggcgtagt ctccatcggg taaaaaacag
      121 atgtcctggc tttctccggt gtgcgcgctt tcaaatcctt cccgtgccgc aatggatcgc
      181 acttctgcct tgctcattcc gccaagggga aaaactgcgc gggcaagctg gtcttgttgt
      241 aaggagtaca atacgtagct ttgatctttt gccggatcaa tagctttttg caacagatac
      301 ctgccggttc gctcgtcctt gcggactctg gcgtaatggc ctgttgcgat cgagtcaaag
      361 ccgagccgta ccgcttcgtc tatcagggcg ccgaatttta tgtggcggtt gcattgtacg
      421 cacgggtttg gggtatcgcc atccaggtat gtttgaataa agttgtcgat tacctgctcc
      481 cggaatattc catgaatggt gaggacccga tgctctatac caagccgttg ggcaactttt
      541 tgtgagtcgg caatagcctt tgttgccgga cagggttgag cagagccgga aacggattga
      601 tcaaacaaat caagggtgca gccagaaacc tcgtgtccct gatccattaa taaagtgcaa
      661 gctaccgagc tgtctactcc accactcatg gcgaccaaaa tacgtgacat atcgttattc
      721 cttgtcccca cctaaaagcc gttcaagaga cagctctctg ccaaaggggc ggcatgcccg
      781 ggtgggtata ctgtgcgtct gtgtcttttt tatagccctg ataaggatgt tctttggcgt
      841 gtggctcata tcgataaact ccagaagctg tacgcgatat ccatattgtt ccaacacagc
      901 cgcccgggag gcgtcggtaa gaagggctgc gatgcgctcg cgtacaagcc catgtttaag
      961 agcatacgcc agctcttgtt ccgctggact ggatgtgtcc ttttcggcca ggcgggcgtt
     1021 aagttcatgt tggcaacagg gaacggcgag aatggccttg gacccccaac tgacagcctt
     1081 cgccagggcc gcatcggttg cggtatcgca ggcatgaagg ctaataacga catcgggcgg
     1141 ggtgtcgcag ctgtaatgtt caatgtctcc ggttaaaaaa cgtagtccgg aagagccata
     1201 gcgttgaaca agagcctggc aatggtcaat tacgtctttt ttaaggtcga gccccgttac
     1261 tctcgccggt atgccccgaa ccttggtaag aaagtgatgg gtggcaannn nnnnnnntga
     1321 ggtacgattt accacagcca aaatccacta cttcaagaac cctgttttcg tccagcccgg
     1381 cgactacctc gtcaatgata tcgtcaataa attccagata gcggtttatc tggcgaaatt
     1441 ttggggtcat cgcctttatt agattgccct cggccgtcat caccccaagg tctactaaaa
     1501 aaggaacggg gtcgtcctcg gacaacagat attttttttc ccggttatga gcgcttgcct
     1561 ggcggcaggt agcttcttcc cgaataaggg cggtaagact gccttttcgg ttagacaaaa
     1621 aactgatagt cccttgggcg cactgggctt cggctcgctt aaaaagcgag gggaatactt
     1681 ctatcacaaa gttcgagagc tctggagcgc tcagattttg ctgaaaggcc ttgttgtccc
     1741 ggattgtttc tttttgaaag gctgagtcgc ttgcggggag ggcgcgaatc cttacccgct
     1801 ctccatgggg aagttcccct tttttaggtg cgctcagcgt cagggatact aatggcccca
     1861 ggggtccctc cccggcggca agacgcgcaa taaattcgga cgggttatca attttaagat
     1921 tcatgcttgt ccattaaaaa gttaagggtt acacaggttt caaaaagcgg catgggggtg
     1981 gcaaggacgg gaattcccgc agcctcggca tgaagtcgca tcgatggatc ggggagtttg
     2041 tcgttgacca caaggactgc gcttgcacca accatgtctg cgcaacgaac gctttgatcg
     2101 gtggcaagac cggtaaccaa gagggcttcg gtttccacca tcactaaaat gtcgctcatc
     2161 aagtcgccgg cgataacgcg ggtaatggcg atgttttgcc atccttgcgg gccccacacc
     2221 aggtgcgccg gcaattcctc gatgatcgtt tttaaggtca caccgtctcc gcgtcctgat
     2281 agtcgttcat tgccgatgcg gcaagatagc gatagtgcag ggcgtttttg cggttctttt
     2341 ctacttcttc gtcggtaagg gtgcggacga gctttgcggg acttcccata agaagggaac
     2401 ggggagggaa ggatttcccg ctcgtgacca gggacatggc gcctatgatg gaatcttcgc
     2461 caatcattgc ct
//
LOCUS       DAOQXQ010000214         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47210_length_2472_cov_2.381878, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000214 DAOQXQ010000000
VERSION     DAOQXQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47210_length_2472_cov_2.381878"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PKH81_04380"
     CDS             complement(<1..1095)
                     /locus_tag="PKH81_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ97306.1"
                     /translation="MTVALSNPRVLLLLALLIPVVSFVLFRLYKLRSAISSFVPDPVH
                     ERRLYRSLLARTFFFSLAWILTVIALSGPQWGFEQVATRGEGVALSLVMDVSRSMTIE
                     DMDGDRLYFSATYARNLLASLPSLPVSVVIVKGSAHLAIPLTEDRRSVLSLLSILSPD
                     LVTTSGSNIGAGITAALDSFPPLLESRKVILLCTDGGETRKSLLDACSRVRQEGVTLI
                     ILGVASSQGGDISVSGSDGVYTHRSVLEESFLQEAVSRCGGDSLFIRGDESGSADVVR
                     RAIMEGSRSSEGMLWQTRPIRRSWLFATLAVICVVLGCIVSGLAGRQLASVFLASATF
                     LFFSGCSFGVSERLAIAQGFFKWTGNDMSGA"
     gene            complement(1092..2081)
                     /locus_tag="PKH81_04385"
     CDS             complement(1092..2081)
                     /locus_tag="PKH81_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ97307.1"
                     /translation="MYFERPALLALLLLIPVKWVLYKTRIFASIRFPLTMGDWQSVPF
                     AWHGPFSFLLSRLSSVCALAAFVCLIFSAASPVRYRQELVFAPSDSTVIFVLDVSPSM
                     AARDMDGKTRLDAARDLISPLVARRSGTAFGLAAVGSSAALLVPPTLDAKVFSDRLSS
                     LAIGEFGEGSALGLALALAASHCTEEGSSIILLTDGENNAGEIHPATGAALISERKGN
                     LIVIGLGSKGQVPLDYLDPVTGKRMTGMLDSDFDEGALRDLAHAGEGSYVSARDLGSL
                     EVVFSGLEKQIPQSPFLWTKGVTDFLDRPFLFASLLFAALAWLVRRLAMGSII"
     gene            complement(2071..>2472)
                     /locus_tag="PKH81_04390"
     CDS             complement(2071..>2472)
                     /locus_tag="PKH81_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97308.1"
                     /translation="FLIYRWCVDHVLPGRSNRRLAVLRKRFLRDIRKLEKRMQKLDRS
                     SWFDAYSTIVRIYLAGEASLDESLAHTHTAREFSLVFSGGSKGYAVGALLEALDYARF
                     SGTTADERQSFIGRARDLCTLCSVEEVQGVL"
BASE COUNT          785 a          671 c          583 g          433 t
ORIGIN      
        1 agctccagac atgtcgttcc ctgtccattt aaaaaaaccc tgagcgatcg caaggcgctc
       61 gctcactcca aatgaacacc cggaaaaaaa gagaaaagta gcggaggcaa gaaaaacaga
      121 agcaagctgc cgccctgcca aacccgagac aatacagcca aggacaacac agattaccgc
      181 aagcgtggca aaaagccagg aacgtcggat aggacgggtt tgccacagca taccttccga
      241 agatcgcgaa ccttccataa tcgcacgccg aaccacatcg gcagagcccg actcatcgcc
      301 gcgaataaaa agactgtcgc cgccacatcg ggataccgct tcttgtaaaa aggattcttc
      361 aagaacagaa cgatgggtgt atacaccgtc gctaccagat acactaatat ctccgccctg
      421 tgaacttgcc acacccagaa tgattagggt gaccccttct tgtcgaaccc tggaacacgc
      481 atcaagcaaa gacttacgag tctctcctcc atcggtacat aaaaggataa cctttcggga
      541 ctccaaaagt ggcgggaagg aatccagagc tgcggtaata cccgcgccaa tattggaacc
      601 gctggtagtt accagatcag gagacaaaat tgataaaaga gaaagaaccg atcgtcgatc
      661 ctcggtgagc ggaatagcaa gatgagcact gcccttgacg attaccaccg atacaggaag
      721 agacggaagg gatgccagaa gattccgcgc gtaggtggca gaaaagtaca gccgatctcc
      781 atccatatcc tctatggtca tactgcggga aacatccatt accaaagaaa gcgccacgcc
      841 ctcaccccgt gttgcaactt gctcaaagcc ccattgcggc ccggatagcg caataacggt
      901 caatatccag gcaagagaaa agaaaaaggt tcgggccaac aaagaccggt ataatcgacg
      961 ctcatgaacc ggatcgggaa caaaggaaga tatagcgctc cgtagcttgt acaaccgaaa
     1021 caatacaaag gacacaacag ggatcagaag agccagaagc aacaacacgc gcgggtttga
     1081 taatgcaacc gtcatatgat tgaccccata gccaggcggc gaacaagcca tgcaagggct
     1141 gcaaataaaa gactggcgaa taagaatggt cgatccaaaa aatcggtaac acccttcgtc
     1201 caaaggaaag gagactgcgg tatctgtttt tcaagtccgc taaacacaac ttccagagaa
     1261 ccaagatcgc gcgcgcttac ataggagcct tcccctgcat gtgccaaatc ccgtagcgcg
     1321 ccctcatcaa aatcagagtc aagcataccc gtcattcgct taccggttac cgggtcaagg
     1381 taatccagag gcacctggcc cttactgcca aggccaataa caatcagatt cccctttctc
     1441 tctgatatca atgcagctcc cgttgcagga tgaatttcgc ctgcattgtt ctctccatcg
     1501 gtaagaagga taatcgaaga tccttcctcc gtacaatgag acgcagcaag ggccagggct
     1561 agacccaagg cactgccctc gccaaactcg ccaatggcca aggaagaaag gcgatcggaa
     1621 aaaaccttgg cgtccaaggt aggagggacg agcaaggcag cagatgagcc aacagcggca
     1681 aggccaaacg cagttccact gcgacgggcc accaacggag aaataagatc ccttgcggca
     1741 tccaatcggg tttttccgtc catatctcgt gcagccattg aagggcttac atccagaaca
     1801 aaaataaccg ttgaatcgga gggtgcaaac acaagctcct gcctatagcg caccggagat
     1861 gctgcggaga aaatcaaaca cacaaaggca gcaagggcac acacagatga taagcgcgag
     1921 agcaaaaaag agaagggacc gtgccatgca aacggaacag actgccaatc tcccatcgta
     1981 agcggaaatc ggatagaagc gaatattctc gttttataca gcacccattt aacgggaatt
     2041 agaagtaaca gagccaaaag ggctggtcgt tcaaagtaca ccctgaacct cctctaccga
     2101 acagagcgta cacaaatcgc gtgcacgccc tataaaggat tgccgctcat cggcggtggt
     2161 tccggaaaaa cgcgcataat caagggcttc aagtaatgca ccaactgcat accccttgga
     2221 tccaccagaa aaaaccaggg aaaactcccg ggctgtgtgc gtatgagcca gagactcatc
     2281 aagactcgct tctcccgcca agtatatgcg aacaatagtg gaatacgcgt caaaccagga
     2341 agagcggtct aacttctgca tccgcttttc cagctttcgt atatcacgaa ggaatcgctt
     2401 cctgagtacg gctaatcggc gattagatcg accgggaaga acatggtcaa cacaccatcg
     2461 atatatcaaa aa
//
LOCUS       DAOQXQ010000215         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47237_length_2471_cov_2.528974, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000215 DAOQXQ010000000
VERSION     DAOQXQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47237_length_2471_cov_2.528974"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKH81_04395"
     CDS             complement(<1..1292)
                     /locus_tag="PKH81_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97309.1"
                     /translation="MNKNAEFGSYLFSSIVPCMIVARNGFVEHWNPASSALFPTLDQA
                     PRIALADLFLQQAHEEDAVRLEYLLKGKEDSFFFDLSQHLTDGSDRWFKLIMTRQSNG
                     KYLAIIDDISTQKIKESHLMAAKENAEQASVTRSQFLANISHEIRTPIQTIIGMMELL
                     TDTRLDEEQTEYARQVRFSADVLLTLINDILDISKVEAGQLKMEDIDFNVVDVIERTV
                     DLISMEGHKKGLEINIDISPSIPVMLRGDPGRLRQVILNLVKNAVKFTESGGLLLKVR
                     IHTHENGVSLDGTALQYLRFEIQDTGIGIPKEAQQKLFTQFFQADASTTRRFGGTGLG
                     LAISKNIVSLMGGEIGVKDNEPTGCTFWFTMPLRPSAIQPPGELLPLNSETRFLLVDD
                     NPQTIKILTGMLISLGYTHVLAALSGKEALETLQTARA"
     gene            complement(1296..2006)
                     /locus_tag="PKH81_04400"
     CDS             complement(1296..2006)
                     /locus_tag="PKH81_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97310.1"
                     /translation="MMTDLSLSFVDGPNLELLLDTILENPSVRKRNVKVLTMDSFAKD
                     FIEGNSSYSEIQAVADLSKAIDSLLKDVNPIEDASTVISERILAPARDGKLSEASFEM
                     RFKSEIQTLKTVGKNIRIAVVDDDVVIRSVLTKTFLAIQANVDQFESGVSFLPQGLSG
                     NYDLIFLDLMMPVMNGFEVLQKLKESGIQTPIIVLSAISQREAVIKVLSSGVKSYMVK
                     PLKPEQLLKKAIEVLQAV"
     gene            complement(2003..>2471)
                     /locus_tag="PKH81_04405"
     CDS             complement(2003..>2471)
                     /locus_tag="PKH81_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97311.1"
                     /translation="SCQTIMEIGLGQPDSLSKMRSLIPDLVIIDYNSAESFIVTLLEQ
                     KKKDPNAVSIPVLVLAQKIDSVTSERLTRLGVKWILPKPLKIDQLLSIMSDVLKCKFE
                     IDTTPCILEAHVNDNILFIEIAQGLNREKIDLLKYSLRELIALYNLTSPRSLS"
BASE COUNT          599 a          527 c          651 g          694 t
ORIGIN      
        1 tgggccctgg ccgtttgcaa ggtttccaag gcttcttttc ccgacaaggc agctaagaca
       61 tgggtgtatc caagggagat aagcatgccg gttagaatct tgatggtttg tggattgtcg
      121 tctaccaaaa ggaagcgggt ttcgctgttc agtgggagta gttctccggg gggctgtata
      181 gcggaggggc gtagcggcat ggtgaaccag aaggtacaac cggttggttc attgtccttt
      241 accccgattt ccccgcccat aagggataca atatttttgg aaatggcgag tcctaggccg
      301 gtaccaccaa agcggcgggt tgtggaggcg tcggcttgaa aaaattgggt aaacagtttt
      361 tgctgtgctt ctttgggtat gcctatgccg gtgtcttgaa tctcgaaccg caggtattga
      421 agggccgtcc cgtcaaggga cacaccgttt tcgtgcgtgt gaattcgtac cttgaggagt
      481 aagccccctg actcggtaaa tttgacggca tttttaacca gattaagaat tacctgcctc
      541 agtcgtcccg ggtcgccgcg aagcatgacc gggatggaag ggctaatatc aatattgatt
      601 tcaaggccct ttttgtggcc ttccatggag ataagatcaa cggtgcgctc tatgacgtca
      661 accacgttaa aatcaatgtc ttccatcttg agttgacccg cttccacctt gctgatgtct
      721 aggatgtcgt taatcaaggt gagcaaaaca tccgcactaa agcgaacctg gcgggcgtat
      781 tctgtctgtt cttcgtccag gcgggtatct gtcaggagct ccatcatacc gattatggtc
      841 tggatcgggg tgcgaatttc atggctaata ttggcaagga actgacttct ggtaacacta
      901 gcctgttccg cgttttcctt tgcggccatt agatggcttt cttttatttt ttgtgtagag
      961 atgtcatcga tgatggcaag atacttgcca tttgattggc gcgtcatgat gagcttaaac
     1021 caacggtcgc ttccatcggt aaggtgttgg gaaagatcaa agaagaatga atcttctttg
     1081 ccctttaaca ggtattcaag gcgcacggcg tcttcttcgt gtgcctgttg caaaaagagg
     1141 tcggccaggg cgattcgggg cgcctggtcc agagtgggga acaatgcgct tgaagccgga
     1201 ttccagtgtt caacaaatcc gttgcgggca acgatcatac aggggacgat cgaagaaaaa
     1261 agataggaac cgaattccgc gttcttgttc atcacctata ccgcctgaag gacttcgatt
     1321 gcctttttaa gaagctgctc tggtttcagg ggcttgacca tatagctttt aacccctgat
     1381 gaaagaacct tgataaccgc ttcgcgttga ctgattgccg aaagaacgat aatgggtgtt
     1441 tgtattccgc tttccttaag tttttgcaat acctcaaacc cgttcataac cggcatcatt
     1501 aaatcaagga agataaggtc gtaattaccc gacagtcctt gcgggagaaa tgagacaccg
     1561 gattcaaatt gatccacgtt tgcctgaatg gccaagaagg ttttggtgag tacagaccgt
     1621 attactacat cgtcgtcgac cactgcgatt cggatgtttt taccaactgt ttttagggtt
     1681 tgaatttcgc ttttaaaacg catttcgaaa ctggcttcag aaagttttcc atctcgggcc
     1741 ggggcaagga tgcgctcgct tataaccgta gaggcatcct ctatgggatt aacgtcttta
     1801 agaagagagt cgatagcctt gcttaaatcg gcaaccgctt gaatctcgct gtaggagctg
     1861 ttcccttcaa taaaatcctt ggcaaagctg tccatggtta gcaccttgac gtttcgcttg
     1921 cggactgagg gattttccag gatggtgtcc aaaaggagtt caagatttgg tccatccaca
     1981 aaggacaggg ataggtcggt catcatgata aggaccttgg gctggtcaga ttataaaggg
     2041 caatgagttc cctgagtgag tatttgagta agtcaatttt ttcccggtta aggccctgtg
     2101 ctatttcgat aaagaggatg ttgtcgttaa catgggcttc caggatgcag ggtgtggtat
     2161 caatttcaaa tttgcattta aggacgtcgc tcatgatgga taataattgg tctatcttaa
     2221 gcggtttggg caatatccat ttaaccccaa gacgtgttaa tcgttcgctg gtaacgctgt
     2281 ctattttttg agcaagaacg agaaccggaa ttgatacggc gttggggtcc ttcttcttct
     2341 gctccagaag ggtaacgata aaggattccg cagagttata gtctatgata acaagatcgg
     2401 ggataaggga gcgcatcttg gaaagactgt ctggctggcc aaggccgatt tccataatag
     2461 tttggcagct a
//
LOCUS       DAOQXQ010000216         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47361_length_2467_cov_3.203980, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000216 DAOQXQ010000000
VERSION     DAOQXQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47361_length_2467_cov_3.203980"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKH81_04410"
     CDS             <1..604
                     /locus_tag="PKH81_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97312.1"
                     /translation="AASALYDLPVAGVVYMQFKKDLPEMPRILASGRVSTDKKQNTTA
                     KQYRQALLDIYGDISRAPPDNVALLNSLVAEETEDADRYIRRDYLERSPAQIQSEGTK
                     ILLEVEDIFNPNIALYPNPTGDCGWQCALQSVCVAMDDGSDFESLLESLTVQRKEEDT
                     SWRQLLPLANQVPQLPPFGALPQAGTPQLEPPQDFLAEWL"
     gene            664..1449
                     /locus_tag="PKH81_04415"
     CDS             664..1449
                     /locus_tag="PKH81_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97313.1"
                     /translation="MYGNYGTGKTRLAGSAALVPQMRDVLMIDAEAGDLTIATQEDTA
                     EHIDVVRVSTFKELARVQEYLKVHCKLRDEDNEEKLKELEKLLFTDYDPDKKARRFNT
                     VIIDSLSEAETFSMYQLLGITENTRLDEETQMAEWAEYKKNNSQILRLVRAFRDLPMN
                     VIITSAAAYTQDEFKRFVYQPALTGKLSKQVQGFMDIVGFLVVTADGSGEDVRRMFVQ
                     PSAKYDAKNRLSSYKGSYFDNPSMGSILQAVDLLEKPNTTKKA"
     gene            1490..1930
                     /locus_tag="PKH81_04420"
     CDS             1490..1930
                     /locus_tag="PKH81_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HNQ97314.1"
                     /translation="MQFEKDESNATMTNLGGVTEDAGPECMPRGVYDVIIDEVTYGIS
                     QRSGNPMWTWKFEVEEGDYANRKLFFHTPFIENSLPRLKKILGRIAPELIETPFDPEA
                     LANEAYFSGKRCRVRVDISKYEGQNRNSVKDVLPPSEEGASGFM"
     gene            2100..>2467
                     /locus_tag="PKH81_04425"
     CDS             2100..>2467
                     /locus_tag="PKH81_04425"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNQ97315.1"
                     /translation="MEGKIKGFVLFSGGIDSTTCLALACNRHGDDNVTAVGINYGQRH
                     AKELEQARQICALYGVEQVILDLGTQPQSMLTNPDADIPNASYKELVGISPTYVHFRN
                     GQMLSKIAAYASSKLADDETA"
BASE COUNT          649 a          602 c          636 g          580 t
ORIGIN      
        1 ggctgcatct gcgctgtatg atttacccgt agcgggtgta gtctatatgc agttcaaaaa
       61 agatttgcca gagatgcctc gtattctggc ttcgggcaga gtttcgacag acaagaaaca
      121 aaataccacg gctaagcaat acagacaggc attgctggat atctacggcg atatctctag
      181 agcacctcct gacaatgttg cgctgttaaa tagcttagtg gccgaagaaa cggaagatgc
      241 cgacagatat attcggcggg attatctcga aaggtctccg gcacagattc agtccgaagg
      301 caccaagatc ctgctggaag tcgaggacat ctttaaccca aacattgctc tttatcccaa
      361 tcctacaggt gattgtggat ggcaatgcgc gctacagtcg gtttgtgtag ctatggacga
      421 tggcagtgac tttgaaagtc tcctggaatc attaactgta caacgtaagg aagaagacac
      481 ctcatggcga caactcctgc ccctagcaaa tcaagtcccg caactcccgc cgttcggagc
      541 gctcccgcag gcgggaaccc ctcagctgga accccctcag gacttcctgg cggagtggct
      601 gtaaaggctc ctccttttca gatttcgtcg atacataaca agagccgttg gctaaagctc
      661 tttgtgtatg gcaattatgg tactggcaaa actagattgg ctggttcggc ggctctggta
      721 ccgcaaatgc gcgatgttct tatgatcgat gccgaagcag gagatctgac aatcgccacc
      781 caggaagata cagcggaaca tattgatgtt gtgcgcgtat caacgtttaa ggaattagcg
      841 cgtgtacagg agtacctcaa ggtgcactgt aaattgcgcg atgaagacaa tgaggagaag
      901 ctcaaggagc tggaaaaact tttgtttacg gattatgatc cggataaaaa agcacgacgc
      961 tttaataccg tgatcattga ttctttgtcc gaagccgaaa ccttctcgat gtatcagctg
     1021 cttggtatta ccgagaatac tcgcctggac gaagaaacac agatggccga gtgggcggag
     1081 tacaaaaaga acaatagcca gattcttcgt ctggttaggg ctttccgtga tctacccatg
     1141 aatgtcatca ttacgtcagc ggctgcatac acccaggacg aatttaagcg attcgtctat
     1201 cagccggctt taacgggtaa gttatcgaag caagtccagg gatttatgga catcgttggc
     1261 ttcttagttg taacagcgga cggtagcggc gaagatgttc gtcgtatgtt tgtccaacct
     1321 tcagctaagt atgatgccaa gaatcgtctt tcgtcctata agggcagtta cttcgacaac
     1381 ccctcgatgg gatcgatctt acaggcggta gacctgttgg aaaagcctaa cactacaaag
     1441 aaggcttaag ccgtaatcta ccaaccaacc aaccaactaa ggaaatgcca tgcagttcga
     1501 aaaagacgaa agcaacgcaa ccatgaccaa tctcggtggc gttacggaag atgcgggtcc
     1561 tgagtgcatg cctcggggtg tgtacgacgt catcatcgat gaagtcacct acggcatcag
     1621 ccagcgttcg ggcaacccga tgtggacgtg gaagttcgag gtggaggagg gcgattacgc
     1681 taaccgtaaa ttgttcttcc acaccccgtt catcgagaac tccctgccgc gactgaaaaa
     1741 gatcctcggt cgtatcgctc cggagctcat cgagaccccg ttcgatccgg aagctctggc
     1801 caacgaggct tacttctccg gtaagcgttg ccgagtgcgt gtcgacatct cgaagtacga
     1861 gggacagaat cgcaacagcg tgaaggatgt tcttccgccc agcgaagaag gtgcttccgg
     1921 cttcatgtga tgtcgcctgt caggtgcaat gcctgactgc tgacttatac gtctttggta
     1981 aatagccaaa gagccggata acgtaaccgg cacccttttt gattggagct gtgctaatgc
     2041 aagctttcga tccgttggaa gtacaaacat ttggtcctat tcggacactg gaggcctcca
     2101 tggaaggcaa gatcaagggc tttgttttat tcagtggcgg cattgacagt acaacttgtc
     2161 ttgcccttgc gtgcaatcga catggagatg acaacgtaac ggctgttggg atcaattacg
     2221 ggcagcgtca cgccaaagaa ttagaacaag cacgccagat ttgtgcattg tacggagtcg
     2281 agcaagtcat cctggatctc ggtacacaac cccaaagcat gctcacgaat cccgacgccg
     2341 atatcccgaa tgcctcgtac aaggagctgg ttggcatttc tcctacgtat gtgcactttc
     2401 gtaatggcca aatgctgtcg aaaatcgctg catacgcatc gtccaagctc gcggatgacg
     2461 aaacggc
//
LOCUS       DAOQXQ010000217         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47492_length_2462_cov_3.621105, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000217 DAOQXQ010000000
VERSION     DAOQXQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47492_length_2462_cov_3.621105"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKH81_04430"
     CDS             <1..460
                     /locus_tag="PKH81_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97316.1"
                     /translation="EPLDEWFFPQFHRFHTLNLVLNWKPANGWTVSLMGSFATGMPKE
                     EVGEITMYPVLYNGQVIERYRRSSFYSDTLRTEFSCPVDLRLSYSNYFKNSKLRWEYY
                     VGAEDILVNLYKPKGNTQFNPYTGKEIKDSDSADFNIGVPMFSVGYKLSY"
     gene            485..1255
                     /locus_tag="PKH81_04435"
     CDS             485..1255
                     /locus_tag="PKH81_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97317.1"
                     /translation="MKQRSVVYNVILIIALSCAALSACRSAPPAEELFWKDLETRTEG
                     DTSIARLRLHRGSETWIEGPVEWKDDGSAILSVEKINWFSNWYNGWTEAEMAAGGKLV
                     IQGEKGKWSVEAQEFLSPHYAQSAKIRYKDNFLSGTQAAELLDRRILRISRAAAFLHE
                     KLEGKTFTRLAIDKKKYRHLGFYQAAGEVLFPEVYGYPSGTAKGNTFVRGEGIRWDAE
                     YSSQQVPPELAEVRNSGTLYRDWEEMPELFYYIYTLEN"
     gene            1259..1909
                     /locus_tag="PKH81_04440"
     CDS             1259..1909
                     /locus_tag="PKH81_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNQ97318.1"
                     /translation="MENEWSLIALFKLGGPFMWPLLAFSIATVALAVERVVYLFWHNM
                     KVSDLKDKSLSLIRDGRISEAVSELAKEPRKNTSATILLSVVQNAEQGEARMEKAAEA
                     EAQERIRRCENGFNYLTALSSIAPLTGFLGTVSGMIGAFKSIASATEVNAQLVANGIY
                     EALITTVFGLMIAIVALVSYNLMIQRVDTFAAEITKAVSDLVSAILKKQEQGAKGQ"
     gene            1906..2313
                     /locus_tag="PKH81_04445"
     CDS             1906..2313
                     /locus_tag="PKH81_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ97319.1"
                     /translation="MIRLRKRREVDDPSNSGTLNDLSFLLIIFFIVIAGFNVNKGFLM
                     TLPDKERPRIVQTDELMRCVLDAEGTLLVDGQAVTMEELDSKAQEMKGKYPNMTFLLT
                     IHPDAPYRQVIGVIHEIRALDIENFSFKMAGGE"
     gene            2313..>2462
                     /locus_tag="PKH81_04450"
     CDS             2313..>2462
                     /locus_tag="PKH81_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ97320.1"
                     /translation="MIQLNRKKKRPMVPTSSMSDIGFLLLIFIMLISLINQRHEVKID
                     YPEGKL"
BASE COUNT          682 a          570 c          608 g          602 t
ORIGIN      
        1 agagcccctt gacgaatggt tctttcccca atttcaccgc ttccataccc ttaacctggt
       61 tcttaactgg aaacctgcca acggatggac ggttagtctg atggggagct ttgctaccgg
      121 tatgcccaag gaagaggttg gggagattac catgtacccg gtcttgtata acggtcaggt
      181 catcgagcgc tatcgcagaa gcagttttta cagcgacacc ctcaggacag aattctcttg
      241 cccggttgac cttcgccttt catactctaa ctatttcaag aactccaagc ttagatggga
      301 gtactatgtt ggggcagaag atattttggt taacctgtat aaacccaagg ggaacacaca
      361 gtttaatccc tacaccggaa aagaaatcaa ggatagcgat tcggcagact ttaacatcgg
      421 agttccgatg ttctccgtgg gctataagct cagttactaa taatacggaa gataaggaac
      481 agatatgaaa caaagaagtg ttgtatataa tgtgatcctg attattgcgc tatcctgcgc
      541 agcccttagt gcttgccgtt cggcgcctcc ggccgaagag ctcttttgga aagatctgga
      601 aacaagaact gaaggcgaca catcgatcgc acggttacgc cttcatcgtg gaagtgaaac
      661 ctggattgaa ggaccggttg aatggaagga tgacggaagc gctatccttt ctgtagaaaa
      721 aatcaattgg tttagcaact ggtacaacgg atggaccgag gccgaaatgg cagcgggagg
      781 aaagctcgtc atacagggag aaaaaggaaa atggtctgta gaggcccagg aatttctttc
      841 cccccattat gcccagagcg caaaaatccg ctacaaggat aacttccttt cggggaccca
      901 ggccgcagaa ctgttagacc gaaggatact tagaataagt cgtgctgccg ccttcctcca
      961 cgaaaagctt gaaggtaaaa cctttacgcg acttgcgatc gacaagaaaa aataccggca
     1021 ccttggtttt tatcaagccg ctggagaggt gctattcccc gaggtgtatg gctatccttc
     1081 aggaacagca aagggaaaca catttgtaag gggcgaaggt attcgctggg acgcagaata
     1141 ttcatcccaa caggtacctc cggaacttgc agaagtaaga aatagcggca ccctataccg
     1201 cgactgggaa gagatgccgg aactgtttta ttatatttat acattggaga actaaactat
     1261 ggaaaacgaa tggagtctta tcgcgctttt taaattgggc ggaccgttta tgtggccgct
     1321 cctcgccttc tctatagcaa cggttgccct tgctgttgaa cgggttgtgt atctcttttg
     1381 gcacaatatg aaggtatccg atctaaagga taaatcactt tctcttatcc gcgacggtcg
     1441 tatttccgaa gcggtatccg aacttgcaaa ggaaccgcgc aagaatacct ccgcgactat
     1501 ccttctttcg gttgtacaga acgcagagca aggcgaagcc cgcatggaaa aagccgcaga
     1561 agcagaggcg caagagcgca tcagaagatg tgaaaacgga tttaactacc tgaccgcact
     1621 ctcttctatc gcgcccctga ccggcttttt gggaacagtc tccggtatga ttggagcatt
     1681 caagtcaatc gcgagtgcca ccgaggtaaa cgcacagctt gtagcaaacg gtatttacga
     1741 agcgcttatt accaccgtat tcggcttgat gatagccatt gtggcccttg tttcatataa
     1801 cctgatgatt cagcgggtag acacctttgc cgcagagata acaaaagcgg taagcgatct
     1861 ggtgtccgcc attcttaaaa aacaggaaca gggagcaaaa ggacaatgat acgcctccgt
     1921 aaacggagag aggtggacga tccgtccaat tcggggacgc ttaacgatct gtcgttcctt
     1981 ctgatcatct tctttatcgt tattgccggt tttaacgtca acaagggttt tttaatgacc
     2041 ctgcccgaca aggagcgccc acgcatcgta caaactgatg aactgatgcg ttgtgttctg
     2101 gatgcggaag gaaccttgct cgtggacggg caggcggtaa cgatggaaga actggacagt
     2161 aaggcacaag agatgaaggg gaagtatccc aacatgacct ttctcttaac tattcatccc
     2221 gacgcgccct accggcaggt aattggtgtt attcacgaga ttcgagcgtt agacattgaa
     2281 aatttctcct tcaagatggc gggaggcgag taatgattca acttaatcgc aaaaaaaaga
     2341 gacctatggt accgacttcc tctatgtcgg acattggctt tctcctgctt atatttatca
     2401 tgctcatttc gcttatcaat cagcgccatg aagttaaaat cgactatcct gagggcaaac
     2461 tg
//
LOCUS       DAOQXQ010000218         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47532_length_2461_cov_3.115960, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000218 DAOQXQ010000000
VERSION     DAOQXQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47532_length_2461_cov_3.115960"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2461
                     /gene="polA"
                     /locus_tag="PKH81_04455"
     CDS             <1..>2461
                     /gene="polA"
                     /locus_tag="PKH81_04455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNQ97321.1"
                     /translation="LFKEHSPELFIAAFDSRKPTFRHEMYDQYKATRQKTPEDLHAQV
                     PVIEEILTALGLALLRQDGFEADDIIASVATRCRAEGRPCIIISGDKDLMQLVGPSVS
                     ILKPGKTGGWDAVDEQGVRSEWGVGPELMLDLLSLTGDASDNVPGVKGIGDKTALKLL
                     ESYGTLDGIYAHADELTGAIGKKIVEGKDMAYFSRKLIALCEDVPIDRDLDAYSCHTL
                     DTLAAGRLLMREGLPSIAKLYNSKAGAEEGESTKIASSNEPSLPQVHPLSGEVTQTTL
                     EKNKGNYRAVSDAASLEAYIQKGCTAGCIAFDCETTGLDVAKDSLVGFSLSHTIGEGI
                     YVPLALPQAELGSDRDQVMEKEKAFELLTTLFAHPKVLIVMHNGKFDLQVLGRHGIFQ
                     NMEGRRPQSKLFDTMIAAWLLDPDWASFSLESLSQSQLSLETIAYDEVVHKGSTFQDV
                     PLAQATQYAAEDADLTLRLYQRFAPRLKEAGLDTLFTTMEMPLVPILAQMETAGIRIE
                     KEALSSYSEELTKEIESTEQRIFAEVGHSFNVASPKQLQEILFTERKLTPGKKTKTGY
                     STDNSVLENLAAEDVLPGMILDYRALAKLKSTYVDALPLLADTEGRIHTSFIQTGTAT
                     GRLSSRDPNLQNIPIRDENGRRIRSAFRAEKGFHLISADYAQIELVILAHLSGDKNLV
                     DAFTAGVDVHRRTASLIFGISESEVTADMRRVAKTINFGVMYGMSAFRLSNELRIPRG
                     QAATFINTYFQTYSGVSSFIEETKKRARASGYVETLMGRKRYIRSINSSNKLEQAGAE
                     RIAVNTPIQGSAADIVKTAMIR"
BASE COUNT          684 a          667 c          576 g          534 t
ORIGIN      
        1 ctttttaagg aacattcccc ggagctcttt atcgcagcct ttgattcgcg aaaacctacc
       61 tttcgccatg aaatgtacga tcaatacaag gctacgcgac aaaaaacccc cgaagacctc
      121 catgcccaag tacccgttat agaagaaatt cttaccgcct tgggcctggc cctgctccgt
      181 caagacggct ttgaggcgga cgatatcatt gcctcagttg caacccggtg ccgagccgaa
      241 ggaagacctt gcatcattat ctccggagac aaggacttga tgcagctcgt tggcccttca
      301 gtatccatcc tcaaacccgg aaaaacaggg ggatgggatg cggtagatga acagggagtt
      361 cgttcagaat ggggggtagg ccctgaattg atgttggacc tcttatcgct taccggggac
      421 gcttccgaca acgttcccgg ggttaagggg attggggata aaacagccct gaaactactg
      481 gaaagctatg gcacccttga tggaatctat gctcatgcag atgaacttac cggcgcgatt
      541 ggtaaaaaaa tcgtagaggg taaggatatg gcctactttt cacgaaagct tatcgccctg
      601 tgcgaagatg tcccgattga tcgggatctt gacgcgtatt cctgccacac cctggacacc
      661 ctcgccgcag gaagactact catgcgggag ggcttaccct ccatcgcaaa actgtacaat
      721 agcaaagccg gagccgaaga gggtgagtct accaaaatcg cttcttccaa tgagccatcc
      781 ttgccacagg ttcacccgct ctccggagaa gtgacccaaa ccaccctgga aaaaaacaag
      841 ggtaattacc gcgcagtctc agatgccgcc agcctcgaag cctatataca aaagggatgc
      901 acggccggct gtatcgcctt tgactgcgaa actaccggcc ttgatgttgc aaaggacagc
      961 cttgtcggct tttccctttc ccataccata ggcgaaggca tctatgtacc gctcgctctc
     1021 ccccaggcag aactgggaag cgatagagac caggtaatgg aaaaggaaaa agccttcgaa
     1081 ctacttacta ctctatttgc acaccccaag gtccttatcg tgatgcacaa tgggaagttt
     1141 gatcttcaag ttcttggccg tcacggtatc ttccagaata tggaaggaag aagaccgcaa
     1201 agcaagttat tcgacaccat gatcgccgca tggcttctcg atcctgattg ggcttccttc
     1261 agtttggagt cactatccca atcgcaactt tctcttgaaa caatcgcgta tgacgaagtt
     1321 gttcacaaag gctcaacgtt ccaggacgtc ccccttgcac aggccaccca atatgccgcc
     1381 gaagacgccg acttgactct caggctttat caacgattcg cccctcgact aaaagaagcc
     1441 ggcttggaca cgctttttac cacgatggaa atgcccctgg taccaatcct ggctcaaatg
     1501 gaaacagcgg ggattcgcat agaaaaggaa gccctttcct cgtattcaga agagctcacc
     1561 aaagaaattg aaagcacaga acagcgcatc tttgccgagg taggccattc ttttaacgtt
     1621 gcctctccaa aacaactcca ggaaatattg tttaccgaac ggaaactcac tccaggaaaa
     1681 aaaacaaaaa caggatactc gaccgacaat tccgtcctgg aaaacctcgc cgcggaagac
     1741 gttcttcccg gtatgatcct cgattaccga gcccttgcaa agcttaagtc aacgtatgta
     1801 gatgccctcc ccctcctggc cgataccgaa ggaagaattc acaccagctt tattcaaacg
     1861 ggaacggcaa ctggacgact ttccagcagg gatcctaatt tacaaaacat tccgatacgc
     1921 gacgaaaatg gcagaagaat ccgtagcgcc ttccgggccg aaaaaggctt tcacctcatc
     1981 tcggctgact acgcccagat tgaactggtg atcctggccc atctttccgg cgacaagaac
     2041 ctggtagacg cctttaccgc gggagtggat gtacaccgcc gtaccgcaag cctgatcttt
     2101 gggataagcg aaagcgaggt aaccgcagac atgcgccggg tagcaaaaac catcaatttt
     2161 ggggttatgt acggcatgag cgcattcagg ctctccaatg aattgaggat tccccgtgga
     2221 caagcggcga cctttatcaa tacctacttc caaacctatt ccggagtaag ctcatttata
     2281 gaagaaacaa aaaaaagagc gcgtgcctcc gggtatgtag aaaccctgat gggaagaaaa
     2341 cgctacatcc gttcaatcaa tagttccaat aaactggagc aagctggagc agaaagaatc
     2401 gcggtaaaca ctcccattca aggttctgcc gcagatattg ttaaaactgc catgattcgc
     2461 g
//
LOCUS       DAOQXQ010000219         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47681_length_2456_cov_2.899625, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000219 DAOQXQ010000000
VERSION     DAOQXQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47681_length_2456_cov_2.899625"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..2123)
                     /gene="feoB"
                     /locus_tag="PKH81_04460"
     CDS             complement(120..2123)
                     /gene="feoB"
                     /locus_tag="PKH81_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNQ97322.1"
                     /translation="MKARIALAGNQNCGKTTLFNQLTGSNQHVGNFPGVTVEKKEGRL
                     REDPEMTVVDLPGIYSLSPYTPEEIVARDFLIHEKPDVLINIIDATNIERNLYLTLQL
                     LTTELPMVIALNMMDEVRANGGVIDIPALEATLGVFIVPIVASKNEGLTELIERVRDT
                     LEGKPIGQNPCPRRLDFCAGPVHKALHSISHLVEGPSRLSGYASRFAATKLIEGDPLL
                     EKELDLITADKDIIKRIIREMEIELGTDREAAIADMRYTFIEGLVATHVVKKEESREQ
                     ARSIRLDSVLTHSVYAIPVFIAVMSTIFWITFGPIGSFVSDLFSVGVDWLISLVNQGL
                     TVAQVSPWMRSLIVDGALTGVGSVLSFLPVITILFLFLSLLEDSGYMARVAFVLDSVL
                     RKIGLSGRSFVPMLIGFGCSVPAIMSTRTLSGSRDRKMTILLIPFMSCSAKVPVYGMF
                     CAAFFPRYTGLVMTSLYFGGMFVAIIAGLIFKRTFFKGGAVPFVLELPVYRLPSFSTV
                     FYHLRGKVMDFVQKAFTIIFFGSLVIWALTNLDWSFHMVEDSSTSILASLGSFIAPIF
                     VPNGFASWQAVSALVSGFAAKEAVVSTLSVVLPAGIEAYFSVPGALSFLVFTLLYTPC
                     VAAVAATRREMGSARWTVLAVLFQTGTAWLVSAVVFQCARLFF"
     gene            complement(2120..2335)
                     /locus_tag="PKH81_04465"
     CDS             complement(2120..2335)
                     /locus_tag="PKH81_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNQ97323.1"
                     /translation="MKPGEAGIITIVGGEGSLRRRLLDMGLTPGTRVSVRKVAPFGDP
                     MELSLRGYELTIRGEDAKSIKCNKGAV"
BASE COUNT          769 a          616 c          496 g          575 t
ORIGIN      
        1 tcaggttcac attttgatcc ggaactggtt cgggtcttct tgtccaatga aaaagctttt
       61 gaaaaaatcg cagaaacaga gcaacgattt cttgaacgcc acaacgagtc agattacccc
      121 taaaaaaaca gacgggcaca ttgaaacacc acagctgata caagccatgc ggttcccgtc
      181 tgaaacaaaa cggccaatac tgtccatcgt gcggatccca tctcccttct tgtagctgca
      241 acagcggcaa cgcatggggt atagagcaag gtaaaaacca ggaatgacag agcaccagga
      301 acagaaaaat acgcctctat acccgcggga agcacaaccg acaaggtact gacaaccgct
      361 tcctttgcgg caaatccgct caccagggca gagaccgcct gccaggaagc aaacccgttt
      421 ggaacaaaaa tcggagcgat aaaagacccc aaagaagcca agatgctggt tgaactgtct
      481 tcaaccatat ggaatgacca gtcaagattg gttaaggccc aaataaccag ggatccgaag
      541 aatataatgg taaaagcctt ctgcacaaag tccataacct ttccccgcaa atgataaaac
      601 actgtggaaa aggacgggag tcggtaaacg gggagttcca gcacaaaggg tactgcgcct
      661 cccttaaaaa aagtacgttt aaatatcaag ccggcaatga tcgcaacaaa catgccgcca
      721 aaatataacg atgtcataac aagccctgta taccgaggga aaaatgcggc acagaacatc
      781 ccatacaccg gaaccttggc cgaacaagac ataaaaggaa taagcaggat tgtcatcttc
      841 cgatcgcgcg agccagaaag ggtccttgta gacataatcg ctggaaccga acagccaaaa
      901 cctatcaaca taggtacaaa agatctacct gataatccaa ttttccggag aaccgaatca
      961 aggacgaagg ctactcgagc catatatcca gaatcttcca aaagagatag aaaaaggaaa
     1021 agtatagtaa ttactggtaa aaaggaaagc acgctgccga caccggttaa ggctccatcc
     1081 acgataaggg atctcatcca tggactaacc tgcgcaactg taagcccttg attaacaaga
     1141 gaaatgagcc aatccacacc aacagagaaa agatccgata caaagctgcc aatcggacca
     1201 aaggtaatcc aaaagattgt agacataacc gcaataaaca ccggtatggc atacacagaa
     1261 tgagttaaca cagaatcgag acgaatcgat cgagcctgtt cccgggattc ctctttttta
     1321 actacatgtg ttgccaccaa gccttcaata aaggtgtagc gcatgtctgc aatagcagcc
     1381 tctctgtcgg taccaagctc aatctccatt tcccgaatta ttctcttaat aatatccttg
     1441 tccgctgtaa tcaaatctaa ttctttctct agtagtggat ctccttctat aagcttagtc
     1501 gcggcaaaac ggctcgcata tccagataat cgagacggac cttcaacaag atgggatatg
     1561 gagtgcagcg ccttatgaac tggacctgcg caaaaatcaa gtcgccgcgg acacggattt
     1621 tgccctatcg gctttccctc aagggtatcg cgtacccgct caatcaattc agtcaatcct
     1681 tcatttttgg atgctacaat tggaacaata aacacaccaa gggtcgcttc aagggcgggg
     1741 atatcgatta cgccaccatt tgcgcgaacc tcgtccatca tattcagtgc aatcaccatt
     1801 ggaagctcag ttgttaacaa ctgtagggtt aagtacaaat tcctttcaat attagttgcg
     1861 tcaataatat ttataaggac atcaggcttt tcatgaatca aaaaatcccg ggctacaatt
     1921 tcttccgggg tataaggaga cagggaatat attcccggta agtcaaccac agtcatctct
     1981 ggatcttccc gcagtcgacc ttcctttttt tccactgtta caccgggaaa gttgccaaca
     2041 tgttgattac tcccggtaag ttgattaaac agcgttgttt ttccgcaatt ttggttccct
     2101 gccagagcaa ttcgggcttt catacagccc ccttattgca tttaatgctt ttggcgtcct
     2161 cacctcgaat ggttaattca taacctcgta agcttaattc catcgggtct ccaaatggtg
     2221 ctactttcct cacagaaacc cgtgtacccg gggttaatcc catatcaagc aaccgccggc
     2281 gtaaactacc ctcgccgcca actatagtaa taatcccggc ttcccccggc ttcatttgat
     2341 ttaaagtcat ttgttaacct cgtattacac caaagtacaa gatttttgtt gtttagtcaa
     2401 ttattatagg gtatagtcat taccatgccc gattctatta gcgcgtccaa acagga
//
LOCUS       DAOQXQ010000220         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_47786_length_2453_cov_2.071726, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000220 DAOQXQ010000000
VERSION     DAOQXQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47786_length_2453_cov_2.071726"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKH81_04470"
     CDS             complement(<1..735)
                     /locus_tag="PKH81_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HNQ97324.1"
                     /translation="MAKGQSSSRPRVRDLEQQIRYHQGLYYNGDPEITDAQFDALWDE
                     LKELDPSNILFNTINSEFTDGFPKEYHLIPMGSQEKAADAESFSAWAASCGFDEFLVQ
                     YKLDGASLELQYEKGILTHAVTRGDGTIGDDITANARKMKGVLAFLPDPWGPSSCTPF
                     SGGIRGEVIMEKAVLATYFPDKANCRNAANGLMKRKDGNGCEHLRVVCYDAASGTPGQ
                     PFSGYTPFNDEEEKIRWLSTCGFDTVP"
     gene            complement(771..1451)
                     /gene="trmB"
                     /locus_tag="PKH81_04475"
     CDS             complement(771..1451)
                     /gene="trmB"
                     /locus_tag="PKH81_04475"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNQ97325.1"
                     /translation="MEDSDQKTAIDHLRQVKTYVLRAGRMTEAQKRDYVIHAARWCLP
                     FAERILNFTDVFENTAPVTMEIGFGMGRATAIIAAENPDKNYIGVEVHRAGVGRLLGE
                     IERRGLTNLRIIEYDALEVLSSMIPNESLSGFHVFFPDPWPKKRHHKRRLVQRPRTDL
                     FASKLAPGGYFYMATDWEPYGEEAYIELTATPTMESMYEGYAPRQTWRPETRFEVKAL
                     RRAGKFTN"
     gene            complement(1463..2161)
                     /locus_tag="PKH81_04480"
     CDS             complement(1463..2161)
                     /locus_tag="PKH81_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97326.1"
                     /translation="MAFPVTRNKIPLLLVCLCALLLFACAQGSPVIKNRSIKLLRVQD
                     PTRSFSEHLSVFVCFEDSDGEEDFSSITITHDESTLFWEITPQEAVVRLRDTERWTGS
                     NNLSYPADGLFPPGRYTLVVADLAGNEAVESFSLSRISFPEYAPAALTIAEGTWTIER
                     SADAAGFTRVFLYLFSQDDRLLYSYLAPDGSQTVTGTVAELLALAPGAVRVQCYIENF
                     SGSAGVLLTAVDLQ"
     gene            complement(2174..>2453)
                     /locus_tag="PKH81_04485"
     CDS             complement(2174..>2453)
                     /locus_tag="PKH81_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97327.1"
                     /translation="TQGKLQITLYNKEDQSEAVRESINDKKNADLKKKSLIDFYKEIP
                     EGEEDTSLGMYYLSYLSEACEKVNVRFESNANQFRDSDLTVINLTFVF"
BASE COUNT          676 a          611 c          546 g          620 t
ORIGIN      
        1 gggaacagta tcaaagccac aggtggaaag ccagcgtatc ttttcttctt cgtcgttaaa
       61 tggggtataa ccggaaaaag gttgcccagg agtcccggat gcggcatcgt agcacaccac
      121 gcgcaagtgc tcgcagccat taccatcctt gcgtttcatt agtccatttg cggcattgcg
      181 gcaattagcc ttatcaggaa aatacgtagc caaaacagct ttttccataa tgacttcacc
      241 gcgaattcca ccagaaaatg gagtacacga agaaggaccc cagggatcgg gcaaaaacgc
      301 taacacgcct ttcatctttc gggcattggc ggtaatgtca tcgccaatgg tcccatcacc
      361 acgagttacc gcatgggtca aaattccctt ttcgtattga agttccaggc ttgccccatc
      421 aagtttatat tgaacaagaa attcatcaaa gccgcaggaa gcagcccagg cggaaaagga
      481 ttctgcgtct gcagccttct cctgactccc cattggaata aggtgatatt ccttgggaaa
      541 gccgtcggta aactcagagt tgattgtatt aaaaagtata ttagaaggat ctagttcttt
      601 cagttcatcc cacagcgcgt caaattgagc gtctgtaatt tcaggatctc cgttataata
      661 gagcccttga tgatatctga tttgctgttc caaatcgcgc accctgggtc gcgatgatga
      721 ttgacctttg gccatgaacg attagtcctc caggcttttt tttctgaaca tcaattcgta
      781 aatttccctg ctcgcctcaa tgccttaacc tcaaaccggg tttccggtcg ccaggtttgt
      841 cggggagcat agccttcgta catactttcc atagtgggag tggcagtgag ctcaatataa
      901 gcttcttcac catagggttc ccagtcggtg gccatgtaaa aatatccacc gggagcaagt
      961 tttgaagcga acaaatcggt gcgaggtctt tgaaccaaac gacgtttatg atgtctcttt
     1021 tttggccaag gatcaggaaa aaaaacatga aaacccgaaa gggattcatt ggggatcatg
     1081 gaagaaagaa cttccaatgc gtcatactca ataatacgaa gattagtcag gcctctgcgc
     1141 tctatttcgc ccaaaagacg cccgacccct gcacgatgaa cctcaacccc aatataattc
     1201 ttgtccggat tctctgcggc gatgattgcc gtagcgcgac ccatgccaaa accaatttcc
     1261 atagtaaccg gggctgtgtt ttcgaacaca tcggtaaagt tgagaattcg ctccgcaaaa
     1321 gggagacacc aacgtgctgc atgaataaca taatcgcgct tttgcgcctc ggtcattctg
     1381 ccagcacgca atacgtaggt tttaacctgc ctgagatgat caatggcggt tttctgatcg
     1441 ctatcttcca tttctaccca ttttactgta aatcaaccgc cgtcaacaat accccggcag
     1501 aaccagaaaa attttcgata taacactgaa cgcgcaccgc ccctggtgca agagcgagta
     1561 attccgccac ggtccccgta accgtctgtg aaccatcagg ggctagatac gagtataaaa
     1621 gccgatcatc ctgagaaaaa agatacaaaa acacacgggt aaaacctgct gcgtcagcgc
     1681 ttcgctctat agtccacgta ccctctgcaa tagtcagggc agcgggagca tactcgggga
     1741 aggaaattct gctcagtgaa aacgattcaa ccgcctcgtt tcctgccaaa tcggctacca
     1801 ccaaggtata ccttccggga gggaataggc cgtctgcagg ataagagaga ttattgctcc
     1861 ccgtccatcg ctccgtgtct ctcaaccgaa ccaccgcctc ttgcggggtt atctcccaaa
     1921 acagggtgct ttcatcatgg gttatggtaa ttgaggagaa gtcttcttcc ccgtcggagt
     1981 cctcaaaaca aacaaacaca gaaagatgtt cagagaagga acgagttgga tcttgcacac
     2041 gaagaagctt aatagagcga tttttaataa cagggcttcc ctgcgcgcat gcaaagagta
     2101 acaaagcaca aaggcatacg agaagcagtg gtatcttgtt ccgcgtaaca gggaaagcca
     2161 tatataagtt atcctaaaac acaaaggtca gattgataac cgttaaatcc gaatcgcgga
     2221 attgatttgc attggattca aagcggacat taaccttctc gcaggcttca cttaaatacg
     2281 acagataata cataccaagg gaggtatctt cctctccctc cgggatctcc ttgtaaaaat
     2341 cgataagcga cttcttttta agatcggcgt tttttttgtc attgatgctt tcgcgtaccg
     2401 cttcgctttg gtcttccttg ttatacaggg taatttgtag cttgccctga gtt
//
LOCUS       DAOQXQ010000221         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_48223_length_2439_cov_2.867450, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000221 DAOQXQ010000000
VERSION     DAOQXQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48223_length_2439_cov_2.867450"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="PKH81_04490"
     CDS             <1..1930
                     /locus_tag="PKH81_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ97328.1"
                     /translation="ADLRSGEPLGSLPAGRLFTLIQGCVNSNFAFADLKALLLDRLIP
                     WKDRRMAEALIAFGIENHCVSSWKENGKPVDIWSEAFAHPARNLLPHPDLPGWFRELR
                     NSLQGLLRSEDFSTLRKKYFAFRERFLDMEQLEEEDDAVFARCVEELKGLIQLTDRYP
                     RLMPHNPWSFFISSLASARYVPQRSSGGVSVFPWRVAAGTPFDYHFVLDASQDRATVL
                     YRELGFLAQDTRARCGLRDSDASLDFFTLYHLCGARFSVSRQAIAGYSTPHGHLREKK
                     PEALALSDPLTAEKAFCFGQGPLPDRLYHSQVSGRNAFMERRVSSRFSYLSSPFGQSV
                     PALTSRITLKHIQDGLVKISQSDLASFAPCNARWFLKHLLGLKQQERDATLPNERNLG
                     LLYHQVLCAVYTRIKKEDKQFNPEHLPEYLEWARAEARGATSQHADFKGPLAEPLIGI
                     LANNVVEGVSKIIQKDAEELPFYEPWALEQKYTVATDTLLFQGLVDRISCNNAEETFV
                     IIDYKTKKKHGVAAYTPTRDSDGRPRMDDYQIPMYLFLAEQNLRKQQIDDAWFADITE
                     GKFHRIVSAQGTGRAKPIERRNFTPAMEALAQMSALFNQAVREADFTKPQDLSREDCA
                     TCDFTEVCRRIYTVGGCE"
     gene            1927..>2439
                     /locus_tag="PKH81_04495"
     CDS             1927..>2439
                     /locus_tag="PKH81_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ97329.1"
                     /translation="MKSIKDLLAELDSSQRDAATMTRNAVIAAGAGSGKTRVLAARYA
                     YLVVEKGIPVHEILTLTFTKKAASEMYARIHQYLSHIDDERATTAVTSFHTARIETID
                     AFCNAIARSACRLWGFSPDFTIDNDRARDIAQSIALPFFIERRSRIAIPALMIRTRLE
                     DIPVNFFANTL"
BASE COUNT          612 a          683 c          569 g          575 t
ORIGIN      
        1 ggcagacctc cggtcaggcg aaccgcttgg cagccttccg gctgggcgcc tttttacact
       61 aatccaggga tgcgtcaact ctaactttgc ctttgcagac cttaaggctc tcttgctcga
      121 ccggcttatt ccctggaaag accgccgtat ggccgaagcc cttatcgcct ttggaataga
      181 aaatcattgc gtaagctcat ggaaagagaa cggtaaaccg gtggacatct ggagtgaagc
      241 cttcgcccat ccggctcgga atctcttacc gcatcccgat ctgcccggct ggttcaggga
      301 attacgaaac agcttgcaag gactactgcg cagcgaggat ttttctacac tccgcaaaaa
      361 gtattttgcc tttcgcgaac ggtttttgga catggaacaa cttgaggaag aggacgacgc
      421 agtttttgcc cggtgcgtag aagagctcaa gggccttatc caacttaccg accgctaccc
      481 ccgcctcatg ccgcacaatc cctggtcctt ttttatcagc tctcttgctt cagcacgata
      541 cgtaccgcag cgaagttccg gcggagtaag cgtttttccc tggcgagtcg ctgcggggac
      601 tccctttgac tatcactttg ttctggacgc cagtcaggat cgcgcaacag tcctctaccg
      661 ggaacttggc tttttggctc aggacactcg agccagatgc ggcttgcggg acagtgatgc
      721 gtccctggat ttttttaccc tgtatcatct ctgtggagcc cgtttttccg tttcacgcca
      781 ggcaatcgcc ggatactcaa cacctcacgg gcacctaagg gaaaaaaaac cggaagctct
      841 tgctctaagt gaccctctta ccgcggaaaa ggctttttgt tttggacaag gaccacttcc
      901 cgatcgcctg tatcattcac aggtttccgg cagaaacgct tttatggaac gtcgcgtttc
      961 ttcccgtttc tcttatcttt cctcgccgtt tggtcagtca gttcctgcac tgacttcacg
     1021 aatcacttta aaacacatcc aggatggact tgttaagata agccagagcg acttggcgtc
     1081 ctttgcaccc tgcaatgccc gatggtttct taaacacctt cttggtctta aacagcagga
     1141 gcgggatgca accttaccca acgagcgcaa tcttggcctc ttgtatcatc aggttctttg
     1201 cgcggtgtac acacggatca agaaagaaga caaacaattc aatcccgaac atcttcccga
     1261 atacctggaa tgggcacgtg ccgaggcaag gggcgcaact tctcaacacg cggattttaa
     1321 gggccccctg gccgaaccgc ttattggaat tctggctaac aatgttgtag agggcgtaag
     1381 caagataatc caaaaagatg ccgaagaact ccccttctat gaaccatggg ccctggaaca
     1441 aaaatacacg gtagctaccg acaccctctt gtttcaggga ttagttgacc gtatttcctg
     1501 taataacgca gaagagacct ttgttatcat tgactataaa accaaaaaga aacacggggt
     1561 tgcagcatac acccccacac gggatagcga cggacgtccg cgaatggacg actatcaaat
     1621 ccccatgtac ctttttttag cggaacaaaa cttgcgcaag cagcagatag acgacgcatg
     1681 gtttgccgat attaccgaag gaaaattcca ccgcatcgta agtgcacaag gcaccggaag
     1741 ggcaaaacca attgagagac gcaattttac accggcaatg gaagcccttg cccagatgtc
     1801 agcgctgttt aaccaggccg ttcgcgaagc agactttacc aaaccgcaag acctgagccg
     1861 cgaagattgc gcgacctgcg actttaccga ggtttgccgc cgaatctaca cggttggagg
     1921 ctgtgaatga aatccatcaa agacctcctg gcagaacttg attcttccca acgcgacgcc
     1981 gccacaatga cgcgaaacgc ggtaattgcc gcaggcgcag gctcaggcaa aacgcgcgta
     2041 ctcgccgcca gatacgccta tctggttgtg gaaaaaggca tcccggtaca cgaaattctt
     2101 acccttacct ttaccaaaaa agccgcttcg gaaatgtatg cacgcataca tcaatatcta
     2161 agtcacatcg atgacgagag agctacaaca gcagttacct catttcatac tgcacgaatc
     2221 gaaaccatcg atgccttttg caacgccatc gcgcgcagcg catgtcgtct ttggggcttt
     2281 tcgccagatt ttaccatcga caacgatcga gcccgcgaca tagcccagtc catcgccctg
     2341 ccttttttta ttgaacgtag aagcagaata gccattcctg ccctgatgat tcgtaccagg
     2401 ctggaagata ttccggttaa cttctttgcg aacaccctg
//
LOCUS       DAOQXQ010000222         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_48441_length_2432_cov_2.870425, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000222 DAOQXQ010000000
VERSION     DAOQXQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48441_length_2432_cov_2.870425"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKH81_04500"
     CDS             <1..252
                     /locus_tag="PKH81_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97330.1"
                     /translation="EVLPEGGTLTEVQQESVAKDMTGSIEYSDAQNGEADGEHNKQAP
                     LPQRTLIVVGSLILLACLTFAGLAVARAKKRREEQERPN"
     gene            268..1758
                     /locus_tag="PKH81_04505"
     CDS             268..1758
                     /locus_tag="PKH81_04505"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HNQ97331.1"
                     /translation="MKRPLLILFFASLAILFIWLWSNRDSLSSAREARKVAKTIVPLQ
                     EGLNREGKATDNEITSIDESRIQPLVPLENDESFLQAISMDVNKDGITDQICAVKSIS
                     EQHLLIVAGLQNPLTGEYTRLQAIRTGVSQGRTLLMYAADVIGDRSQCLVFSGMTSDN
                     TQLLSVFLPVTDKDGRVHLQVIADLRSDGSILVKEVQRTDAYNLGVTSGESFPITVYS
                     SDPDSPESLAQIERIYRWDRNVQRYEKASEAKIPGKRLERRLVDQLQSGNIESFEQYL
                     NGLWYMTSSIAGKGPRYVFFDSPMKEIVFHNEITEEIFTREAATPRRLGAYLTTRNRS
                     ISSIRRMIDIELTGIDEIKIKILEDVKLKIGVASDWDGIYRKMSGMSSGTRESPDEMT
                     TKLGSLLSGRDAGWKGDDGTQYQFTQGIYTKTDSIGKEMGSYALLTVKGLPVLQMRPD
                     DRNKTARFYRVETEVDNAKQKVILTEVSVSIDGTKLAGIAPIILRP"
     gene            complement(1769..>2432)
                     /locus_tag="PKH81_04510"
     CDS             complement(1769..>2432)
                     /locus_tag="PKH81_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ97332.1"
                     /translation="DTLVRKVMLTSFSEPVLIRARLYSAWIQLSTSSSAQALSLLRDF
                     SRLPSYSPWLPAILFTLWWSDSDADALSRLKAECPTSVEASIVTGSIQTAPVPFWYLM
                     ARNQERVNAFAREGSQDLAGKLAARSPEPPAKAEKPQTPKVSSSSQERLWQQVGFFKN
                     VEYAQELSMRLKKLEFTCIIREEKRPSGTTYYSVLVSEDASHGSAARLKDAGYESFLV
                     IE"
BASE COUNT          711 a          572 c          617 g          532 t
ORIGIN      
        1 gaggtacttc cagagggagg aacattgacc gaagtacaac aagagagcgt agccaaagac
       61 atgactggct caattgagta ttcggacgcc cagaacggag aagccgatgg ggaacacaac
      121 aaacaagccc ccctacccca gagaaccctc atcgtggtag gatcgctcat actccttgct
      181 tgtcttactt ttgcgggact cgctgtagct cgcgcaaaaa aaagacggga agaacaggaa
      241 agaccgaact aacagacagg gacaccgatg aaacgaccgc tcctcatcct tttttttgcc
      301 agtcttgcca tattattcat atggctctgg agtaaccggg acagtttatc ctctgcacgg
      361 gaagcacgaa aagttgccaa aaccattgtt ccgctccagg aagggttaaa ccgcgagggg
      421 aaggctaccg acaacgagat tacctccatt gatgaatccc ggatacagcc attggttccc
      481 ctggaaaatg acgagtcctt ccttcaagca atttccatgg atgtaaacaa agacggcatt
      541 accgatcaga tatgcgcggt caaaagcatc agcgagcaac accttttgat tgttgccggg
      601 ctgcaaaatc ccctgaccgg tgaatataca cggctacagg cgattcgaac cggcgtctcc
      661 cagggaagga ccctgcttat gtatgccgct gatgtcattg gggaccgaag ccaatgcctc
      721 gttttttcgg ggatgaccag cgacaacacc cagctgctat cggtattctt gcctgtaacc
      781 gataaggacg ggcgagtaca tttgcaggta atcgcggatt tgcggtcgga tgggtcaatt
      841 ctggtaaaag aagtacagcg aacagatgct tataatcttg gtgttaccag tggagagagc
      901 ttccccatta cggtatacag ttcagatccc gattcgccgg aaagccttgc tcaaattgaa
      961 cgaatatatc gctgggacag gaatgtgcaa cgctatgaaa aagccagtga agctaaaatt
     1021 cctggtaaac gactggaaag acgactcgtt gaccaattac aaagtggaaa tattgaatca
     1081 tttgaacagt atctcaatgg cctttggtat atgacttcct ccatagcagg gaaaggccct
     1141 cgctatgttt tttttgacag tcccatgaag gaaattgtat tccataacga aataaccgaa
     1201 gaaatattta cccgggaagc ggcaacacct cgacgccttg gcgcctattt aaccaccaga
     1261 aaccgatcta tctccagcat tagacgcatg atagacattg aattaaccgg gattgacgag
     1321 atcaagatta aaatactgga agacgtaaaa ttaaaaattg gcgttgcatc cgactgggat
     1381 ggtatttata gaaagatgtc agggatgtcg agcggtacca gagaaagccc tgatgagatg
     1441 acaacaaaac tgggaagcct cttaagtggc agagatgcag gctggaaagg cgatgatgga
     1501 acacagtatc agtttaccca gggaatctac accaaaacag acagcatcgg taaagaaatg
     1561 ggttcctacg cactgcttac cgttaaagga ttaccggtac tgcaaatgag gcctgatgat
     1621 cgcaataaaa cagcgcgttt ttaccgcgta gaaacggaag tggacaacgc gaaacaaaag
     1681 gtcatcctga ccgaggtgtc agtatcaatt gacggtacaa aacttgcggg cattgcgcct
     1741 attatccttc gaccctaata gccaaccctt actcgataac taaaaagctt tcgtagcctg
     1801 catcttttaa gcgtgcggcg cttccatggg atgcatcctc gctcaccaga acagaataat
     1861 aggtggttcc gctcggccgt ttttcttccc tgatgataca ggtaaattca agcttcttta
     1921 gacgcatgga cagttcctgt gcatactcta cattcttgaa aaaacccacc tgctgccaca
     1981 ggcgctcttg cgacgagctg gagactttgg gtgtctgggg tttttccgct tttgcgggag
     2041 gctcagggga gcgagcggca agcttaccag ccaggtcctg acttccctcc cgagcaaagg
     2101 cgttaacccg ctcttgattg cgggccatta aataccaaaa gggcactgga gcggtttgta
     2161 tggagcctgt aacaatagaa gcctcgacgg aagtgggaca ttcggctttt aagcgagaga
     2221 gggcgtctgc atcagagtcg gaccaccaca gggtaaaaag aatagcggga agccaggggg
     2281 aatacgatgg aaggcgagaa aaatcgcgaa gcaaagagag cgcttgagct gaggaggagg
     2341 tggaaagctg aatccaggct gaatataatc gggcacggat taacacaggc tccgaaaaac
     2401 tcgtaagcat aaccttccgg accagcgtat cc
//
LOCUS       DAOQXQ010000223         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_48471_length_2431_cov_3.005892, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000223 DAOQXQ010000000
VERSION     DAOQXQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48471_length_2431_cov_3.005892"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKH81_04515"
     CDS             complement(<1..1197)
                     /locus_tag="PKH81_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97333.1"
                     /translation="MRATRIPPCLLLCAVLLVSCASVEHYKEIDDSVSRGDFPEALEK
                     VKKEENEGYREKDAILFYLDAGLLSHYSQRWAESSAYLGKGEKAIEVAFTKSLSQGAA
                     SYLVNDMTKEYDGEDYEDIYLNVFNSLNYYHRGSLDGALVEVRRIDNKLKNLAVKYGQ
                     SITNAQRDLLDKDSGIPFDASVASIRFSNSALARYLSMLFYRADGLFDDARIDRDKVK
                     LAFANQRDMYPFPLPSTLDGELSIPRGMARLNVISFSGLSPAKQEETVRIPLSGSRWV
                     KVSIPVMKKRPTKVARVELTLSTGETLPLELLEDLGAVAQETFRGKAGLIYLKTVLRS
                     VLKTTSSVILEEQSSKASSLEESLILGLLSLGTQAYAEASEQADLRLSRYFSAKAHVV
                     GVTLPPG"
     gene            complement(1204..1818)
                     /locus_tag="PKH81_04520"
     CDS             complement(1204..1818)
                     /locus_tag="PKH81_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNQ97334.1"
                     /translation="MKNRSVFAIVSLFAVLVLVSSCSSNPKVTRVDSDTQIDLSGYWN
                     DTDVRIVCESLINDCLSSPRVARFEQQKGRLPVVIVGSFRNQSDEHIDTSIITKRMET
                     AILNNGKADFVASSAERGELREERQAQQDWSSEETAKALANETGADYMMIGSVKTIVD
                     QAGKTATRTYFVYAELIELESNRKVWLGENSEIKKVIKRPSKKL"
     gene            complement(1897..2253)
                     /locus_tag="PKH81_04525"
     CDS             complement(1897..2253)
                     /locus_tag="PKH81_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ97335.1"
                     /translation="MKSVVIRTTDIQKSITFYEKVLGFIFDSMISAAPGKQIAFLSDG
                     AGSAKLELLYNERNKDICPGGVSLTFVVDQIASAEKHLVANGVRIISAPKTIKDGKKI
                     MTAVDPNGVELDFIEE"
BASE COUNT          627 a          615 c          564 g          625 t
ORIGIN      
        1 accgggggga agagtaacac cgactacatg ggcctttgcg gaaaaatacc gcgaaagccg
       61 taagtctgct tgctcggaag cctcggcata ggcctgggtt ccaagactta acaggccgag
      121 tatcagcgac tcttccaacg acgatgcctt ggatgattgc tcttcaagga ttacagatga
      181 ggtggttttt aacaccgacc gaagcactgt ctttaaataa attaaaccag ctttaccccg
      241 aaaggtttcc tgggcaaccg cgcccagatc ctccagtaat tcaaggggaa gggtttcccc
      301 ggtagaaaga gtcagttcaa cccgtgcgac cttggtgggc cgctttttca taaccgggat
      361 ggacacctta acccaccgtg agcctgaaag cgggatacga acggtctctt cctgttttgc
      421 cggagaaaga ccgctaaagc tgattacgtt tagtcgggcc atcccgcggg gaatggaaag
      481 ttcgccgtcc agggttgagg gaagcgggaa cggatacatg tcccgctgat tggcaaaggc
      541 gagtttaacc ttgtcccggt cgattctggc gtcgtcaaaa agcccgtctg cacgatagaa
      601 aagcatactt aaatagcggg cgagtgccga gttggaaaac cggattgatg cgacggacgc
      661 atcaaaggga atgcctgaat ccttatccaa aagatcccgc tgggcattgg taatggactg
      721 gccgtattta acggccagat tttttagttt attgtctatt cgccgtacct ctaccagggc
      781 tccgtccaga gatccgcgat gatagtaatt aagagagtta aagacattca gatagatatc
      841 ttcgtagtcc tcgccatcgt attctttggt catatcgtta accagatacg aggctgctcc
      901 ctgagaaaga ctcttggtaa aggctacttc aatagccttc tcgcccttac ccaaataggc
      961 cgacgattca gcccatcttt gtgagtaatg agacagaagg cctgcatcca gataaaaaag
     1021 aatggcgtcc ttttcccgat agccctcgtt ctcttctttt tttacctttt ccagggcttc
     1081 gggaaaatcc ccccgggaaa cagagtcatc tatttccttg taatgctcaa cagaggcgca
     1141 agacaccaga agtactgcgc acagtaacag acaggggggt attcgtgtag ctctcatgcg
     1201 ctcttacagt tttttagacg gtctcttgat aaccttcttg atttcgctgt tttcgccgag
     1261 ccataccttg cggtttgatt caagctcgat caattcagcg tatacaaaat aggtccgagt
     1321 ggcagttttt cccgcctgat caacaatcgt tttaactgag ccaatcatca tgtaatcggc
     1381 tccagtttcg tttgccaggg cctttgcagt ttcttcgctg gaccagtcct gttgggcttg
     1441 gcgctcttcc cggagttctc cccgttcggc gctgcttgcc acgaaatctg ccttgccatt
     1501 attcaagatg gctgtttcca tccgcttggt aataatgctt gtatcaatat gctcatcgct
     1561 ctgattgcgg aaagagccga cgattacgac cggaaggcgt cccttttgct gttcaaagcg
     1621 ggcaacccgg ggagaagaca ggcaatcatt gataagcgat tcgcagacga tgcgaacatc
     1681 ggtatcgttc cagtaaccgc ttaaatcaat ttgggtgtcg gaatcaacgc gagtaacctt
     1741 gggattggac gagcaagacg ataccaacac aagaactgcg aacaaggaca ctatcgcgaa
     1801 tacacttctg tttttcatat gttctcctta acatggatgg aaaatagtat agctccaacc
     1861 gggttggaaa acaaagactg tccaacccga tctatcttat tcttctataa aatccagttc
     1921 aactccgtta gggtcaacgg cggtcataat cttttttccg tcctttatgg tctttggggc
     1981 agaaataatg cgaaccccat tggcaacaag atgcttttcc gcgctggcta tctggtctac
     2041 cacaaaggtc agggacacac ctcctgggca gatgtcctta ttgcgttcat tgtacaacag
     2101 ttcaagtttg gcagacccgg ctccatcaga aagaaaggca atttgcttcc ccggcgccgc
     2161 ggaaatcatc gaatcaaaga taaacccaag gactttttcg taaaaagtaa tcgatttctg
     2221 gatatcagta gtcctgataa caacgctttt catgttcacc tgcgtacact ccttatcatg
     2281 catcgtagcg gtaataccat taataatagc ctattacgct tactcctggc aaacaaaacc
     2341 ggcgcctata ccattcataa caccatagaa gtccggaaat gttaccgcgc agcattcgcc
     2401 atccaaaaca gttaccccgg ggccctcaat a
//
LOCUS       DAOQXQ010000224         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_48875_length_2418_cov_3.229369, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000224 DAOQXQ010000000
VERSION     DAOQXQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48875_length_2418_cov_3.229369"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /gene="fliR"
                     /locus_tag="PKH81_04530"
     CDS             <1..788
                     /gene="fliR"
                     /locus_tag="PKH81_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNQ97336.1"
                     /translation="NDLVAKAPIFFLVAVRIFAMISSTPLLSIQSIPRVAKIALAALV
                     AFLVYPFAFTQGWQVEAFSLHYALLVVGEALLGIITGFFISMVFSTFSSAGQFFSYQM
                     GFGASAVYDALAQIENPLMGQYLNLIAMLVFVQIRGFQTLFLGGVLRSFETINCHVLI
                     THQGVFLTFFLKGLSLLFMDAMIIAMPILGTLFLIHVAMGLLSKAAPQMNLLSEGFPI
                     TILVTFFLLSIALPLMINLFIQIMQNGFIAFEQLLADSGGAIP"
     gene            785..1930
                     /gene="flhB"
                     /locus_tag="PKH81_04535"
     CDS             785..1930
                     /gene="flhB"
                     /locus_tag="PKH81_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNQ97337.1"
                     /translation="MTALTAPDIHYLLGSLDLQWFAAEDEGRTEDPTEYKLRKAREEG
                     RVAKSQELSSALVLLFPVVVLLFTAPSMLKTCTEMLTFYLTRSTSADFSDPALFQAFL
                     RYFIKLLLPVTGTAVVAGIAANILQNGGFIFSLKPIEPKLSKIAPNFARFFSKALFST
                     EGLFNLAKSIVKVSIISLVAYMSIKDELPRLVSMLSAGFWPSVAFVARMSGRLLFTAS
                     LFFVAISVPDYLFQRRQFMESLKMSKQEIKEEYKQMEGDPLVKSRLRQRMRELLSRNM
                     AVNVPKADVIITNPTHYALALQWDRATMRAPMLTAKGADRLALRIREIARESGVPIVE
                     NKPLARALYAEVEIGDIIPDEYYQALAVILAKVYALDSRKARILKEL"
     gene            1940..>2418
                     /locus_tag="PKH81_04540"
     CDS             1940..>2418
                     /locus_tag="PKH81_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNQ97338.1"
                     /translation="MAEKTGSVQKSDLFVAFGAIVVVLMLIIPLPAVILDLFMALNLV
                     LNLLIMLIVLYTRKATDFSVFPTLLLVSTVYGLGLNVSSTRLILSQGVKFEGKMVRAF
                     SSFVIGTSGTEGLVIGFVIFIILIAVQAFVITKGSTRVAEVAARFTLDAMQTKNMAI"
BASE COUNT          545 a          549 c          532 g          792 t
ORIGIN      
        1 ttaatgatct ggttgcaaag gccccgattt tctttttggt tgctgtgcga atctttgcca
       61 tgatctcttc aactcctcta ctttctattc aatccatacc tcgagtagcc aagatcgcac
      121 ttgccgctct tgtcgctttt ttggtgtatc cctttgcgtt tactcaaggg tggcaagtgg
      181 aagctttttc ccttcattac gcgttgcttg ttgtcggaga ggcccttctt ggaattatta
      241 ccggtttttt tatcagtatg gttttttcta ccttcagttc tgcaggtcaa tttttctcat
      301 atcagatggg ttttggcgcc tcggcagtct atgatgccct tgcccaaatt gaaaaccctc
      361 ttatgggaca gtatcttaat ctaattgcca tgctcgtttt tgttcagatt cgcgggtttc
      421 agacgctatt tctaggtggt gtattacgaa gttttgaaac gataaactgt catgttctga
      481 taactcatca gggagtcttt cttacttttt ttcttaaagg tttatcgctg ctttttatgg
      541 acgcgatgat tattgccatg cccatattgg gaactttgtt tttgatacat gtggcaatgg
      601 gtttgctttc caaggcagct ccccagatga atcttctgtc tgaaggcttt cctattacca
      661 tactcgttac ttttttcctg cttagtatcg cacttccgct catgattaac ctctttatcc
      721 aaattatgca aaatggattt attgcctttg aacagttatt ggcagattct gggggagcta
      781 ttccatgacg gccctaaccg cccctgatat acactacctt ctaggctctt tggaccttca
      841 gtggtttgcc gccgaggatg agggaagaac agaagaccca accgagtaca aactcagaaa
      901 ggctcgggaa gaaggaaggg tagccaagag tcaggaacta agttctgccc tggtgctgct
      961 tttccctgtt gtggtgttgt tatttacagc cccttccatg cttaaaacct gtacggagat
     1021 gcttaccttt tatttgactc gctccacaag cgccgatttt tctgatcctg ctctatttca
     1081 ggcttttctc cgttatttta tcaagttgct ccttcccgtt accggtaccg ctgttgttgc
     1141 aggtatagct gcaaacatcc ttcaaaatgg tggatttatt ttttcgttaa aaccaataga
     1201 gcccaaactg agcaagattg cacccaattt tgcccgtttt ttttccaagg ccctgttctc
     1261 taccgaaggt ctttttaatc tggcaaagtc gatcgtcaag gtttctatta tctccctggt
     1321 tgcctatatg agtataaagg atgagcttcc acggcttgtc agcatgctgt cggctggttt
     1381 ttggccatca gttgcctttg tggccaggat gtctggtcgc ctgcttttta ctgcctctct
     1441 tttttttgtc gctatctcgg tacctgatta tttatttcaa cgacgtcaat ttatggaatc
     1501 cctgaaaatg agcaagcagg aaataaaaga agagtataag caaatggaag gggatccatt
     1561 agtaaaaagc cgcttgcgtc agcgaatgcg cgagctgtta agccggaata tggcggttaa
     1621 tgttcccaaa gccgatgtta ttattactaa cccgacccat tatgcccttg ctttacagtg
     1681 ggacagggcc accatgcgcg cgcccatgct taccgcaaag ggagctgatc ggctcgccct
     1741 caggatacgg gaaatagcac gggagagtgg ggttcctata gtagagaata aaccattggc
     1801 gcgcgctctc tatgcggaag tcgaaattgg tgatattata cctgatgagt actatcaggc
     1861 tttagcggtt attctcgcga aggtctatgc gctcgactcc cgaaaagctc gcatactcaa
     1921 ggaactgtaa caaaagcgca tggcagaaaa aactggaagc gtacagaaaa gtgatctctt
     1981 tgtagccttc ggcgccattg ttgtcgtcct catgcttatt atcccgctcc ccgcggtaat
     2041 ccttgattta tttatggccc ttaatctggt acttaatctg cttattatgc ttatcgttct
     2101 gtatactcgc aaggcaaccg atttttcggt gtttcctacg ctcttgcttg tatcaacggt
     2161 gtatggactt ggattaaacg tatcgtctac ccgccttatt ctttctcagg gagttaagtt
     2221 cgagggaaag atggtcagag cctttagcag ttttgttatt ggaacctctg gcaccgaagg
     2281 attagttatt ggttttgtta tctttattat tctaatcgca gttcaagcat ttgttattac
     2341 caagggttca acccgtgtcg cggaagttgc cgccaggttt acactcgatg ccatgcagac
     2401 taaaaacatg gccataga
//
LOCUS       DAOQXQ010000225         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_48886_length_2418_cov_2.498942, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000225 DAOQXQ010000000
VERSION     DAOQXQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48886_length_2418_cov_2.498942"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="PKH81_04545"
     CDS             <1..1956
                     /locus_tag="PKH81_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNQ97339.1"
                     /translation="EEENLPIRACIYDGDTPQSIRQDAREKARLIISNPDMLHTGVLP
                     NHPRWIRFFSNLKYIVIDEMHLYRGIFGAHMANVVRRIRRIARFYGANPCFIFCSATI
                     GNPRSLAQAILGDSVQLIDINGAPSGKKTVVLYNPPLVDAVAGIRRSVMNESQKWALA
                     FLRQGIKTILFAHSRVRTEVIASYINDSLRNVFTANHGINVVPYRGGLLPLERRAIEK
                     GLREGTIQGVVSTNALELGIDIGGLDAAIVAGFPGSFSSFWQQAGRAGRRQTESVAVF
                     IASSSPLDQYIISHPDYFFNRPQETAQLDPDNPCILTDHVKCAAFELPFSDDDEELFF
                     PKESVTDILSFLEEDGIVRHTGGRWHWSARSYPSEAVSLRSATADNVVIVDMSAGRNL
                     VIGEIDRPSAKELLFPNAVYIHLGRQYMVKVLDIPNRRAEVEECEVNYFTDAVVKTDI
                     RVLSEDETSRSGEYPFSIVVGDVLVRSQVAKYKKLRFHTQENIGFGEIALDAEEMETR
                     GMMLLLEENESDNSALPQSYNDLDEAGQSEVLAGLASLIRSVAPFFLLCDRRDLGVVP
                     LVRDIHFGKSALYIYDKAPGGSGLSEALAVRITEVLCAARERTVACGCDGGCPSCIGV
                     DNSGETIKNASIELMGSLTGFTVKERE"
     gene            1956..>2418
                     /locus_tag="PKH81_04550"
     CDS             1956..>2418
                     /locus_tag="PKH81_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNQ97340.1"
                     /translation="MKSDRLASRLQRIRKMEGQGKKHISVTSKHPVQLSGWTEIDDLV
                     YERECTLHVSEYRPYCSPLLPLLFPRERVVLEKIGPLDVSSLLFFDLETTGLSHGAGT
                     VAFMAALASFTAPGELCLRQFVITDFPGEAAFLALLAKETGKNPRLVSFNGK"
BASE COUNT          600 a          531 c          602 g          685 t
ORIGIN      
        1 gaagaggaaa acttgccaat tcgggcatgt atctacgatg gagatacacc tcaatctatc
       61 cgccaggatg cccgggaaaa ggctcggctt atcatttcca atccggatat gcttcatacc
      121 ggtgttttgc caaatcatcc gcggtggata cggttttttt ccaatctcaa atacatcgtt
      181 attgacgaga tgcacctgta caggggaatt tttggcgcac atatggcaaa tgtggtcagg
      241 cgaatcaggc gaatcgcccg tttttatggt gcaaacccct gttttatctt ttgttccgcg
      301 acaatcggca atcctcgatc cctggcacaa gcgatactgg gagactcagt tcaactcata
      361 gacattaatg gagcgccctc ggggaaaaaa acggtggttc tgtataatcc tcccctggtt
      421 gatgcggttg caggtatcag aaggagcgta atgaacgaaa gccaaaagtg ggcgcttgcc
      481 tttttacggc agggtatcaa aaccattctt ttcgctcatt cacgagttcg caccgaggta
      541 atcgcttcat acatcaatga ctctcttaga aatgttttta ccgcaaatca tggaataaat
      601 gttgttccct atcgtggggg cttactccct cttgaacgta gagcgattga aaaaggatta
      661 cgagaaggta cgatccaggg ggttgtgtct accaatgcgc ttgaattagg aatcgacatc
      721 ggggggctcg acgcggcgat tgtcgcaggc tttcccggtt ccttttcttc tttttggcag
      781 caagcgggaa gggcagggag aaggcaaact gagtctgtcg ctgtttttat cgcatcttcc
      841 tcgccgctcg atcagtacat catttctcat ccagattatt tttttaaccg accacaggaa
      901 acagctcagc tagatccgga taatccatgt attttaaccg atcatgttaa atgcgcagcc
      961 tttgaattac cttttagcga cgatgatgaa gaactttttt tccccaagga atcggttacc
     1021 gatattcttt ctttcttgga agaagatgga attgtccgtc atacaggggg tcgatggcat
     1081 tggtctgcac gatcataccc ttctgaagca gtgtctttac gatctgcaac cgcagataat
     1141 gttgttattg tcgatatgag cgctggaaga aatctggtaa ttggggagat cgaccgccct
     1201 tcggccaaag aactgttgtt tcctaacgca gtatatattc atcttggccg tcagtacatg
     1261 gtaaaggttc ttgacattcc aaacaggcga gcagaagtag aagagtgcga agtgaattat
     1321 ttcactgacg ctgtggttaa aactgacatt cgtgtgttat ctgaggatga aaccagtcga
     1381 tccggagaat atccgttttc tattgtggtg ggggatgtat tagttcgctc tcaagttgcc
     1441 aaatataaaa aactacgctt tcatacccaa gagaatatcg gttttggtga aattgccctg
     1501 gacgctgagg aaatggaaac gaggggaatg atgttgctcc tggaggagaa cgaaagcgat
     1561 aattcagcac tacctcaatc atataacgat cttgatgaag ccggacaatc agaggttctt
     1621 gcagggcttg cttcgctgat tcgctctgtt gcgcccttct ttttgctctg tgaccgccga
     1681 gatcttgggg ttgtacctct tgtgcgagat atccattttg gcaaaagcgc cttatatatc
     1741 tatgataagg cgcctggcgg ctctggttta tcagaggcac tggcagttcg aatcaccgaa
     1801 gttttatgtg cggcacggga gcggacagtc gcctgtgggt gcgatggtgg ctgcccttcg
     1861 tgtatcgggg ttgataacag tggagagact ataaaaaacg cttcaatcga attaatgggc
     1921 tctcttaccg gattcactgt taaggaacgt gagtaatgaa atccgatcgt cttgcatcgc
     1981 gactccaacg aatacggaag atggaaggac aggggaaaaa gcacatttca gttacttcaa
     2041 agcatcctgt acagctttcc gggtggactg aaattgatga tcttgtatat gagcgggaat
     2101 gtaccttaca cgtcagtgaa tatcgtcctt attgctcccc tttgttaccc ctgttatttc
     2161 ctcgggaacg ggtagtgtta gaaaagattg gaccgcttga cgtttcaagc ttgctattct
     2221 ttgatcttga gacaaccggt ctttcccatg gtgcgggaac tgttgcgttt atggccgctc
     2281 tggcttcttt taccgctcct ggggaacttt gcctgcgcca atttgtaatc actgattttc
     2341 ccggagaagc tgctttcctt gcgctgctag ccaaagaaac ggggaaaaac ccccgcttgg
     2401 tatcttttaa cggaaagt
//
LOCUS       DAOQXQ010000226         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49002_length_2414_cov_3.684612, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000226 DAOQXQ010000000
VERSION     DAOQXQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49002_length_2414_cov_3.684612"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="PKH81_04555"
     CDS             complement(<1..1456)
                     /locus_tag="PKH81_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ97341.1"
                     /translation="MRSIRFALFLAVFLCSLLVGLPAQDSALIAEDPSVISSVLDNGM
                     TVHLIENKEPANRIMLRLAVRAGSVLEDDDQSGIAHLIEHMAFNGTANFKKNELIDYF
                     ESIGMAFGPEVNAYTSFDETVYMLEIPADNPAIIEKSLLVLHDWACAITFDPDELDKE
                     RGVVIEEWRMGRGANGRVQDKQIPLLFRDSRYALRRPIGDPEIVRTISRERIMDFYRD
                     WYRSDHMSVIAVGDFDVDSMNKAINKALSTVPASKATRAKQEYPVPLQRDPAVLVIRD
                     PEISYTTVQILEQFPAQRLRTLEDYRKRTVESLAVSILNKRLSEKTYEANPLFLASEN
                     GVRRILRQTQFSYVAMVPSAERFNESFTLLLTELDRYILFGASDAELEREKASSLDRI
                     KQLWLDRDKTHSAARAGSLLSSMLSGDAFPSLDERYRLHQEIIPTITREELLESIKGR
                     FTRRGTLLFVTAPEQSPDIPAENELLETWQKWQRP"
     gene            complement(1539..>2414)
                     /locus_tag="PKH81_04560"
     CDS             complement(1539..>2414)
                     /locus_tag="PKH81_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97342.1"
                     /translation="SLSETASVTEQMARNIDSLALAIKNQAAAVTESSAAIEQMISNF
                     ASVSGMTGTTETFVNGLQAHADEGKECLAGVAELVKQVIQSSDNLSSATSLISTIASQ
                     TNLLAMNAAIEAAHAGDAGKGFAVVADEIRKLAEDAAKQAKAINGTIREVKGLIGNVD
                     DRSIQTTKAFEKIDSSVGEVFNLVRQINSAMTEQNAGSTQILEALTSMRDITVLVEQG
                     STEMSVGNSRLLEVFTALKDITVDVNDRMTEIISGIAEMNKAIQQISELGVQNKDTAG
                     IIQVDIERFKVSSVS"
BASE COUNT          686 a          592 c          464 g          672 t
ORIGIN      
        1 caggtctttg ccatttttgc catgtctcca gcaactcatt ctctgcgggt atgtctgggg
       61 attgctcggg tgcggttaca aaaaggagtg tcccacggcg ggtaaacctt ccttttatgg
      121 actcaagaag ctcttctcgc gttattgtag gaattatttc ctgatgcaat cggtagcgct
      181 catcaagaga agggaaagca tctcctgaaa gcattgaaga caacaatgat cccgcccttg
      241 ccgctgaatg cgtcttgtcc cgatccaacc aaagctgttt aatcctgtct aaagagcttg
      301 ctttttctcg ctcaagttct gcatcactcg ccccaaaaag aatataccgg tcaagttcag
      361 ttaaaagaag agtaaatgat tcattaaacc tttctgccga aggaaccatc gccacataag
      421 aaaactgtgt ctgccgtaaa attcttctaa caccattttc gctagccaag aacaatggat
      481 tggcttcata ggttttctcg gatagccgct tatttaaaat agacaccgca agcgattcta
      541 ctgttcgctt ccgataatct tcaagagtcc gcaatcgttg agccgggaac tgctccagaa
      601 tttgaactgt cgtatatgaa atttcaggat cacggataac caatacggcc ggatcccttt
      661 gaagaggaac tggatactcc tgtttagctc gggtcgcttt acttgcagga actgttgaca
      721 gcgccttatt gatggcctta ttcatagaat ccacgtcaaa atcacccacg gcaataactg
      781 acatatggtc gctacggtac caatcccgat aaaaatccat aattcgctca cgggaaatag
      841 tccttacaat ctctggatct cctataggcc ttcttagagc atagcgggaa tcgcgaaaaa
      901 gaagcggaat ctgcttgtct tgaactcgcc cattagcccc ccgacccatt cgccattctt
      961 ctatcacaac tcctcgttct ttatccagct catctggatc aaaggttatt gcacaagccc
     1021 aatcatgtaa taccaaaagg gacttttcta taattgcagg attatctgcg gggatttcca
     1081 acatatatac agtttcatca aaagaggtat acgcattaac ctcagggcca aaggccatac
     1141 caattgactc aaaataatca atcaattcat ttttcttgaa attagctgtt ccattaaagg
     1201 ccatgtgctc aatcagatga gcaatgccag attgatcatc atcctccagc acagaacctg
     1261 ctcgaactgc caaacggagc ataatccggt tagcaggctc cttgttttct atcagatgaa
     1321 cggtcattcc attgtctaaa acagaactaa ttacagaagg atcttctgct attagggcac
     1381 tgtcttgggc gggcaaaccg accaacaagc tacaaaggaa cacagctaaa aataaagcaa
     1441 acctaataga gcgcatgaac acctcgtcag tataaaaaaa taggacgcag acactgtagc
     1501 atctgcatcc ctagtataac aatagaaccg tgaattaatt acgaaaccga actaacttta
     1561 aatcgttcaa tatcaacttg aataatacct gctgtatcct tattttgaac accaagctcg
     1621 ctaatctgtt gaattgcctt attcatctcg gcaatgccgg aaataatttc ggtcatccgg
     1681 tcattaacat cgacggtaat atctttaagt gcggtaaaca cctcaagaag gcgactgttt
     1741 cccacactca tttcggttga tccttgttca accaaaacag taatatccct catcgaagtc
     1801 agagcctcaa gaatttgggt acttcccgcg ttttgttctg tcattgcgct attaatctgt
     1861 cttactagat taaacacttc tcctaccgaa gaatcaattt tttcaaaggc cttggtcgtt
     1921 tggattgaac gatcgtcaac gtttccaata agacctttta cttcgcgaat tgtaccgtta
     1981 atggccttag cttgcttagc cgcatcctct gcaagcttgc gaatttcgtc cgctacaacc
     2041 gcaaatccct tgccagcgtc tccagcatga gcagcctcta ttgcagcgtt catggccaaa
     2101 agatttgttt gactggcaat agtggaaatc aagctggttg cactcgatag attgtcagaa
     2161 ctttgtataa cttgcttaac taactccgca actccggcta gacattcctt accctcatca
     2221 gcatgagcct gtagaccatt tacgaatgtt tcggttgtac cggtcattcc cgatacagag
     2281 gcaaagttgg atatcatctg ttcaatagca gccgaggatt cagttaccgc tgctgcctga
     2341 ttctttattg ccaaagccag ggaatcaata ttgcgagcca tttgttcagt tacagacgca
     2401 gtttcggaaa gact
//
LOCUS       DAOQXQ010000227         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49369_length_2402_cov_3.414146, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000227 DAOQXQ010000000
VERSION     DAOQXQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49369_length_2402_cov_3.414146"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKH81_04565"
     CDS             complement(<1..625)
                     /locus_tag="PKH81_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNQ97343.1"
                     /translation="MIPLNKLERLTPGQQRRKTALLLASFERELLEYMDTDRSYPQAR
                     RDYLLSLLGFVAKDPKITLSMDVQICATATIPTLIRECNRYRHALLQVIGLLPAEWDL
                     VHPSSLRQKEDIDRRFYPGIRAYVEDIRSPFNLGSIFRTAEAFGAELLYLSPLCCQDT
                     HPRAVRSSMGSMNFLPFERTSLDNLPEGFPVFALETGGTPIAEFDFPS"
     gene            complement(637..1785)
                     /locus_tag="PKH81_04570"
     CDS             complement(637..1785)
                     /locus_tag="PKH81_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNQ97344.1"
                     /translation="MADFVFKISPNVVLGPYTMARLGQVALTWGTKYMLIADPILKEF
                     DLVAKAVTALEEKGISVFVFDDIPSAATSSTLEHALSLARGARIHGVISFGGIKTASL
                     GRAIAALFNESHDVSDFLAGAQPYSGALPFIEVPSTCRDPFVFADRTPVVDARNRQIR
                     LVKTQNAVCKAVVIDPNLYMHMSPNTASSMIFHAISLAVEGYVSTKSNFFSETILGKS
                     IEMLLLALDPEQSKLVGTPQEMLIAQGGCMASMGVAISSPGVATAISLACNARYKTST
                     SLVCSVLLPYIMEDASRAKVDRIVTIGKMLGVGSAGLDASEVARAAIDDIRRRLALAG
                     LPTRLKDLGLSIDQLVPAAEDASALDLMNYIPRAMSSDDLFDLIKQAY"
     gene            complement(1914..2195)
                     /locus_tag="PKH81_04575"
     CDS             complement(1914..2195)
                     /locus_tag="PKH81_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNQ97345.1"
                     /translation="MIDRLGGIDPLKNVQNTHKAHKTSEASKPDSISVSAEARELSEV
                     YYALEAAGSSPDVRADRIAEVMEKMKDPNYINRTVVEGVADRLMDVYGL"
BASE COUNT          674 a          600 c          567 g          561 t
ORIGIN      
        1 gagaaggaaa gtcaaactcc gcaattggag tgccgccggt ttccagggca aagaccggaa
       61 acccttctgg caagttatcc aaggatgttc tctcaaaggg aagaaaattc atgctcccca
      121 tggaagatcg aaccgcacga ggatgagtat cctggcaaca cagaggagag aggtacaaaa
      181 gctctgcccc gaaggcctct gctgtcctga aaatcgaacc aagattaaat ggagaccgaa
      241 tatcttccac atacgcgcga atcccgggat aaaatcgtcg atcaatatcc tctttttgcc
      301 gcagcgatga aggatgcact aaatcccact cggcaggcaa taaacctatc acctgtaaaa
      361 gcgcatgcct gtatcggtta cattcgcgaa tcaaggttgg aatagttgct gttgcacaaa
      421 tctgaacatc catcgaaagg gtgatctttg gatccttagc tacaaatccc aacaaagaga
      481 gtagatagtc cctgcgtgcc tgtggatagg accgatccgt gtccatatat tctaataact
      541 cgcgttcaaa cgacgccaag agcaaggcgg tttttctccg ctgttggcct ggggttaaac
      601 gctcgagttt attaagagga atcattggag aaaggcctaa tatgcttgct ttatcaagtc
      661 gaaaagatcg tcgctcgaca tagcccgggg aatataattc attaaatcaa gggcagaagc
      721 gtcctctgct gccggaacaa gctgatcgat agaaagacca aggtccttca ggcgtgtggg
      781 aagacctgcc agggctaaac ggcgacgaat gtcgtctatt gccgcacgag cgacctcgct
      841 cgcatccaaa cccgcagagc caacgccaag cattttacca atagtaacaa tacggtccac
      901 cttggcacgg gaggcgtctt ccatgatgta gggcaataaa actgaacaaa ccagagatgt
      961 ggacgtctta tagcgggcat tacacgcaag agagatagca gttgccacac caggagaact
     1021 gatggcgaca cccatggatg ccatacaacc accctgtgcg attaacattt cctggggagt
     1081 gccaacgagc ttgctttgct ccgggtcaag ggcaagcaag agcatctcaa tcgattttcc
     1141 caaaatggtt tcggaaaaga agttcgattt tgtcgataca tagccttcta cggccagtga
     1201 tatcgcatga aaaatcatgg aagatgctgt attgggcgac atgtgcatat acaggtttgg
     1261 atcgatgaca accgccttgc acaccgcatt ttgggtttta accagtctga tttgtcggtt
     1321 tcgtgcgtcc acaaccgggg tgcgatcggc aaagacaaag gggtcgcgac aggtagaagg
     1381 gacctcgata aagggaaggg cgccgctata gggctgggcc cccgccaaga agtccgaaac
     1441 atcatgactt tcgttaaaca aggccgcaat tgcacgacca agagacgcgg tttttattcc
     1501 gccaaaggaa attaccccat gtatgcgggc tcctctggct aaagacaggg catgttccaa
     1561 tgtagaagac gtagcggccg aaggaatatc gtcaaacaca aacaccgaaa ttcccttttc
     1621 ttccaaagcc gttacagcct ttgccacaag gtcaaattct tttagaatgg gatcggcaat
     1681 gagcatatac ttggttcccc aggtaagggc aacctgccca agacgggcca tagtatacgg
     1741 accaagaacg acattcggcg atatcttgaa cacaaaatct gccatacaaa cctcttgtta
     1801 tctctacgcc aattttagca tcttttaggc aaaaaaaaag gcgggaaccc cgaagggccc
     1861 gcccttatac gttgaacagg gtacactatc ccttcaacga acctttccag gttttaaaga
     1921 ccatagacat ccataagacg atctgcgaca ccctctacga cggtgcgatt gatatagttg
     1981 ggatccttca ttttttccat aacctcggca attctatcag cgcgtacgtc aggagaagag
     2041 ccggcggcct caagggcgta atacacctca gaaagttccc tggcttccgc agatacgctg
     2101 atcgaatcgg gcttggaagc ctcgctcgtt ttatgcgctt tgtgagtatt ttgtacgttt
     2161 ttcaggggat caatgccccc taatctgtca atcatcatac tttcctgcct tcaccgtata
     2221 tatcggtcat tcatcgtcag aaattgagcg tttttttccg gatatataca cggaaaattc
     2281 gccaagaacc ttttcgcggg aagaatagtc ttcccaaaga tccttggaag acccgctcag
     2341 gatctcctca tgcaatttcg tcagttctcg tccaacagta atgtgccgtt cactatctat
     2401 tt
//
LOCUS       DAOQXQ010000228         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49675_length_2393_cov_3.308383, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000228 DAOQXQ010000000
VERSION     DAOQXQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49675_length_2393_cov_3.308383"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKH81_04580"
     CDS             <1..293
                     /locus_tag="PKH81_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97346.1"
                     /translation="EAIQSNPWNEHREGLLGVKRLSGSKKRISGEEEDIMSSLDAYEI
                     PSGAIRLGKDQIVFTFSLWCNPRNLAEEEERVREHLSRRYPEFMELQKTVPR"
     gene            complement(339..1004)
                     /locus_tag="PKH81_04585"
     CDS             complement(339..1004)
                     /locus_tag="PKH81_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97347.1"
                     /translation="MNAKTENGKTTLTGTGTYVDNLGHTESYSGKAYVFGDGKTISCD
                     KYWYANESEALCWGGFDANMTGTASISGTVTIDYNSVNESEFTFTDNADEALDGSCFA
                     VLLHGFNEENMDDNAGIMADCILKSDSPYTYTYELKNVPAGDYYIFFVGVKAGNRIPN
                     SIGEALGLYGLTDPDIAMDWIDEVDLTEPDKTTFDAIRPITVSEGDAITKDFTAFVRD
                     RNK"
     gene            1488..1619
                     /locus_tag="PKH81_04590"
     CDS             1488..1619
                     /locus_tag="PKH81_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97348.1"
                     /translation="MSAERDAYLGELKAASPASEALDTAVLAPLSNLAKDKGFVPAK"
     gene            1639..>2393
                     /locus_tag="PKH81_04595"
     CDS             1639..>2393
                     /locus_tag="PKH81_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97349.1"
                     /translation="MKGLLTRFAIILIMPLLIVPLATLTWLASRSVDQQEIAMRSRLS
                     DTLLLEIGQTNGRIRAWFDELPSLLGASSPPAADSRRPTADELSAWKERDRFVGIPFL
                     LNAEGSIIYPVSSQEESKSEAVRRFFWRYVTVFGSQEPIPVYRNIADEYESSIVKESV
                     PEEIYSFEDAPKAKRAPPAQERSSVRSKVAQSLFESDTDLQRQVYDMADEEGKETLKR
                     NVHPKIDAVVSREANRPRSVIVETSKYFKDLVS"
BASE COUNT          603 a          550 c          571 g          669 t
ORIGIN      
        1 ttgaagcaat tcagagcaat ccctggaatg agcatagaga gggtttgtta ggcgtaaaaa
       61 gattgtctgg cagcaagaaa cgtattagcg gagaagaaga ggatattatg agttcccttg
      121 atgcgtatga aattccttcc ggcgcgatac gcttgggtaa ggatcaaatc gtttttactt
      181 tttctctgtg gtgtaatccg cgcaatctcg cggaggaaga agagcgagta agagagcacc
      241 tgtccagaag gtatccagag tttatggaat tacaaaaaac tgtcccccgg taattgagga
      301 acagtttaat aatgtaggtc ttgagtctgg ttatagtatt acttgtttct atcacggacg
      361 aatgcggtaa agtctttggt aattgcatcc ccttctgata ccgttattgg cctaattgcg
      421 tcgaatgtgg ttttatccgg ttctgttaaa tccacttcat caatccaatc catggcaata
      481 tccggatcag taagcccata cagtccaagg gcttccccga ttgaattcgg gatacggtta
      541 cctgctttaa ctccaacaaa aaatatgtaa taatctcctg cgggcacatt tttaagttca
      601 tacgtgtatg tatagggact atcgcttttt agtatgcaat ccgccatgat acccgcattg
      661 tcatccatat tttcctcatt aaaaccatgg agaaggacgg cgaagcatga tccgtccagt
      721 gcttcgtctg cgttatctgt aaaagtgaac tccgactcgt ttacactatt ataatcaatc
      781 gttactgttc cggatattga agcggttcct gtcatgtttg cgtcgaaacc gccccagcaa
      841 agtgcctctg actcattggc ataccagtat ttgtcgcaag agatagtctt gccgtctcca
      901 aacacgtacg ccttgccgga atagctttcg gtatggccaa ggttatcaac gtaggtaccg
      961 gtaccggtca gtgttgtttt accgttctcg gtttttgcat tcataatgac agtgcattgc
     1021 ttggatttcc gagcgtcatt cacattatcg ttatctgtat catacaggta ataaatgttt
     1081 gttgctatta cgactgaacc gaattgggta agtgttgcct caaaatcata gtaatgaact
     1141 aagtcttcat cggtggtatt ggcttcctcg aaattataca gcgttcctgt gtagacgcct
     1201 tcgatcttgc ctccggtaaa tgtccaagta ctggaagtcc agaccaggca ttcgcctttt
     1261 tttaccgtga ttgtcagttc gtagggtttt ttaatcgtag aggggagtcc gttattcggt
     1321 atctcgaacg aatacttgcc cgttcctgtt tgcgttggag taaacgagaa cggcgaacca
     1381 atggcgatgg gtgttccatc ctttcctgca aagtacgcct gtgcagtata gccatcacct
     1441 gctttccagt cggagtcttt gattttccag gatctgaaat cgcgacgttg tctgcagagc
     1501 gggatgcata tcttggagag ctaaaagctg catctcctgc aagcgaagct ctggataccg
     1561 cagtgcttgc acccttgtca aaccttgcaa aggataaagg atttgtcccc gcaaagtaaa
     1621 cctgatacaa tagccgtcat gaaaggactt ttgacgcgat tcgcgatcat cctcatcatg
     1681 ccgcttctta ttgtaccgct tgcaaccctt acctggctgg catcgcggag cgttgatcaa
     1741 caagagattg cgatgcgctc ccgtctgagc gacacccttc tgcttgagat cgggcaaacc
     1801 aatggaagga tacgcgcttg gtttgatgaa cttccatcgc ttcttggcgc gagctcccca
     1861 ccggctgctg actcgcgccg tccaaccgca gatgaacttt ctgcctggaa ggaacgtgac
     1921 agatttgtcg gcattccctt tcttttgaat gcggaaggct cgattatcta tcctgtgtct
     1981 tcgcaagagg aatcgaagag tgaggctgtt cgccgctttt tctggcgata cgtgactgta
     2041 ttcgggagcc aagagcccat accggtttac cgcaatattg ctgatgaata tgaatcctcc
     2101 atcgtaaagg aatcagtgcc ggaagaaatc tactcgtttg aggatgcacc aaaggcaaaa
     2161 cgggctcctc ctgcacagga gcgatcaagc gtcagatcca aggtagcgca gagcttattc
     2221 gagtccgata cggacctgca gcgtcaggta tatgatatgg ctgatgagga agggaaggaa
     2281 accctaaagc ggaatgtaca tccaaagatt gatgcagtcg tttcccgcga ggcaaacagg
     2341 ccccgctctg tgatagtaga aacaagcaaa tacttcaagg atcttgtctc tca
//
LOCUS       DAOQXQ010000229         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49704_length_2392_cov_2.932820, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000229 DAOQXQ010000000
VERSION     DAOQXQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49704_length_2392_cov_2.932820"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..386
                     /gene="trxA"
                     /locus_tag="PKH81_04600"
     CDS             69..386
                     /gene="trxA"
                     /locus_tag="PKH81_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ97350.1"
                     /translation="MAVLHTNAASFDKVINQDVPVLVDFWAPWCGPCKMLGPVLEEVE
                     KELGTGAVVAKVNVDEEQDLAVKFGVSSIPTMVVFKKGEQVDRTVGFMAKDKIVSLVK
                     SHQ"
     gene            complement(393..1241)
                     /locus_tag="PKH81_04605"
     CDS             complement(393..1241)
                     /locus_tag="PKH81_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97351.1"
                     /translation="MKEIIKYQLISRRKPFLFSLAALLTINGVSWFFGARDLLSNAPQ
                     FSGSSVFWLFIAGVFSSVSLIITVLACGSGHLKSLLFKDTGYLLLSLPRRGWQILAGR
                     FIAGLAEAALFFLAFTLCASVHTVLWPILAMSPDTSFWQVYLERLQSLVSLGVVTHAK
                     IALIMICAFTAVGNLLSFAIIATRSFIHRKGLSWISAAVVFIVISNIATSWGQAISKR
                     FSWYLHIDRAPSLMSRNVLPLSGIQQSHSMSALEVPLAPFALYLIIAVLLFIASSWLL
                     DNKVEV"
     gene            complement(1260..1961)
                     /locus_tag="PKH81_04610"
     CDS             complement(1260..1961)
                     /locus_tag="PKH81_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97352.1"
                     /translation="MTSLLETKELTKNYLGKQALCAVDVHLESGRILGLMGPNGSGKT
                     TFLKILASLIKPSSGSFSVCGASRGPEVKGLVSFLPDRNTLYPWMRAIDAIAFYDDFF
                     ADFNRAKALEMLAFMKLEQSERVASMSKGMIEKLNLTLAFSREAKLFLLDEPLGGIDP
                     VARERIVSTIIRTWSENSSIIISTHLVNDVERLFNDVAFLDKGRILLSGDAESLRAER
                     GTSIDRLYIDIFGDR"
     gene            complement(1958..2326)
                     /locus_tag="PKH81_04615"
     CDS             complement(1958..2326)
                     /locus_tag="PKH81_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ97353.1"
                     /translation="MNDFDATIPIYLQIMNDIKRDIVTGRLGQGAKLPSVRECAEANT
                     VNPNTVQRAYQELEREGVSETRRGMGTFVRDDPELTKRLRQDMARSTTLACLSALTSL
                     GFTNSEILAEIQSALTEESL"
BASE COUNT          638 a          587 c          598 g          569 t
ORIGIN      
        1 agttactgcc acctgccaca ggattctgta tacttatgat atatacagag ttagtaagga
       61 ggctcactat ggcagtacta cacacaaacg cggcgagttt tgacaaggta atcaatcagg
      121 atgttccggt ccttgtggac ttttgggctc cctggtgtgg gccgtgcaag atgcttgggc
      181 ccgtacttga ggaagttgaa aaagaacttg gaacaggcgc tgttgtagcc aaagtaaacg
      241 tagacgaaga acaagacttg gctgtcaagt tcggcgtatc cagcataccg actatggtcg
      301 tctttaaaaa aggcgaacag gtagatcgca ccgtcgggtt tatggctaag gacaaaatcg
      361 tttccctggt aaaatcccac caataacggc ccttacacct ccaccttgtt atccaaaagc
      421 cagcttgagg caataaaaag caggacagcg ataataaggt agagggcaaa tggcgcaagc
      481 ggcacctcaa gagctgacat ggaatggctt tgttgaattc cggacagggg tagtacattc
      541 cggctcataa gggatggggc tctgtctata tgcaggtacc agctaaagcg tttacttatt
      601 gcctgtcccc agctggtggc gatgttagaa ataacaataa acaccaccgc ggcagatatc
      661 caggaaagac ctttgcgatg aataaaagat cttgttgcga ttattgcaaa tgacaagagg
      721 ttccctaccg cagtaaaggc gcatatcatg ataagcgcaa tcttggcatg tgtaactact
      781 cccagtgaaa caagagactg caatcgctcc agatatacct gccaaaacga ggtgtctggc
      841 gacatggcaa gtattggcca aagcacggtg tgaaccgagg cgcagagggt aaaggccagg
      901 aaaaaaagag ccgcttctgc aagtccggct ataaacctgc ccgctaaaat ctgccagccc
      961 cgacggggca gggagagtaa aaggtatccg gtatccttaa aaagcaagct ctttaggtgt
     1021 cctgatccac aggcaagcac cgttataatc aaacttaccg agctaaagac accggcaata
     1081 aagagccaaa acaccgaaga gccggaaaat tgaggggcgt ttgacagtaa atcccgtgca
     1141 ccaaaaaacc atgacacccc gttgattgtc agaagggccg caagagagaa caaaaacggc
     1201 tttcgtcttg aaataagttg atacttgatt atttctttca tgtttcttta tctccttaat
     1261 taccggtcgc caaagatatc tatatagagg cggtctatcg atgttccccg ctcggcccgc
     1321 aggctctctg cgtctccact aagcagtatt cttcccttat ccagaaaggc cacatcgtta
     1381 aacagacgct ccacatcgtt aacaaggtgg gtgctgatga tgatggagga attctcggac
     1441 caggtgcgga taatcgtgga gacgatacgc tcccgcgcta ccgggtcaat gcctccaagg
     1501 ggttcatcca gcaaaaagag ttttgcctct cgcgaaaagg caagggtcaa attcagtttc
     1561 tctatcatcc ccttggacat ggaggctacc cgctcgcttt gttcaagctt cataaaggcg
     1621 agcatctcca gcgccttggc acggttaaag tcggcaaaaa aatcatcgta aaaggcaatg
     1681 gcgtcaatag ctcgcatcca ggggtacagg gtgtttcggt ccggtaaaaa ggaaaccagg
     1741 ccttttacct cgggtccgcg gctggctccg cagacagaga aactcccgct ggagggcttt
     1801 attaaagaag ccagaatctt gaggaaggtg gtctttcccg atccgtttgg ccccatgagc
     1861 cccagaatac gcccgctttc cagatgaaca tctactgcgc acagggcttg cttaccaaga
     1921 tagttctttg ttaattcttt tgtttctaat agagatgtca taaggattcc tccgttaagg
     1981 ccgattgtat ttccgcaagt atctcgctat tcgtaaagcc aagtgatgta agcgcgctca
     2041 ggcaggccag ggtagtgctt cgtgccatgt cctgacgcag gcgtttggta agttcagggt
     2101 catcgcgaac aaaggtgccc atcccccggc gggtttcgct tacgccctcc cgctccagtt
     2161 cctggtacgc ccgttgcacg gtattggggt taacggtgtt agcctctgcg cattcgcgta
     2221 ccgagggaag ttttgctccc tgaccgaggc gaccggtaac gatgtcccgc ttgatgtcgt
     2281 tcatgatttg caggtaaatc ggtattgtcg cgtcaaaatc gttcatcttc cctccgtgtt
     2341 ctagtgtgtt actctactaa tacaccacaa caatgctttt gacaagcctc tt
//
LOCUS       DAOQXQ010000230         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49744_length_2391_cov_2.009846, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000230 DAOQXQ010000000
VERSION     DAOQXQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49744_length_2391_cov_2.009846"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKH81_04620"
     CDS             complement(<1..444)
                     /locus_tag="PKH81_04620"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97354.1"
                     /translation="MAFPEDYGSKDLAGKTAQFEVTLKKVEAPKLPEVNPEFATQLGV
                     PGGDLEKMRADIRANLEREVGQRVSARTKANVMEALPKLAQFELPAALVQAESAQLLE
                     RAKADLQARGMDLKQLPDIPADTFKEQAERRVRLGLVVAEIVRENG"
     gene            640..1125
                     /locus_tag="PKH81_04625"
     CDS             640..1125
                     /locus_tag="PKH81_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HNQ97355.1"
                     /translation="MVRTWCASLCTEILTRSHGIADWKLVNAFIDGIAVSSPDDCRRA
                     LIRYLWRTLTVAGLNPPVDCCPQCGSGVNSENAVVYYSPHEDACVCLSCVGLDEKRFP
                     LSPEARSYLVSIACLKPAESRAIPLGLQAYTELKNFLFFLLSRMIDSPLKTLESGEGI
                     L"
     gene            1159..2391
                     /locus_tag="PKH81_04630"
                     /pseudo
     CDS             1159..2391
                     /locus_tag="PKH81_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683789.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
BASE COUNT          560 a          613 c          672 g          546 t
ORIGIN      
        1 gccgttctcg cggacgatct cggccaccac caggcccagg cgcacgcggc gctcggcctg
       61 ctccttgaag gtgtcggccg ggatgtcggg caactgcttg aggtccatgc cgcgggcctg
      121 gaggtcggcc ttggcgcgct cgagcagctg ggcgctctcg gcctgcacca gcgcggcggg
      181 cagctcgaac tgggccagct tcggcagggc ctccatcacg ttggccttgg tgcgggcgct
      241 gacccgctgg ccgacctcgc gctcgaggtt ggcgcggatg tcggcgcgca tcttctcgag
      301 gtcgccgccg ggcacgccca gctgggtcgc gaactcgggg ttcacttcgg gcagcttggg
      361 cgcctcgacc ttcttgagcg tgacctcgaa ctgcgcggtc ttgccggcca ggtccttcga
      421 gccgtagtcc tcggggaagg ccaccgggaa gcctgtttat tcacggaaga taatactctt
      481 gttcgggccg ccgtttttgg cggggctaaa agcaagctca gatccctggt ttctccctgg
      541 caccgcggca gagtctggat ctatagcgat ccggtaaaaa aaacggccaa gataaccgat
      601 tttgacgtac aagctttcca cgaagggatc agggaagatt tggtccgaac ctggtgtgcg
      661 tctttatgta cggaaatact gacccggtct cacgggattg ccgattggaa attggtaaat
      721 gcctttatcg atggcattgc cgtatcaagt ccggatgact gccgacgagc gcttatccgg
      781 tatctatggc gaaccctgac agtcgccggc cttaatccac cagttgattg ctgtccccaa
      841 tgtggatctg gcgtgaacag tgaaaatgcg gtagtatact attcaccgca cgaagatgcc
      901 tgtgtatgcc tttcgtgtgt cggactcgat gaaaaacgat tccccttatc accggaagca
      961 cgttcttacc tcgtgtccat cgcttgccta aaacctgccg agtctcgagc tataccgctc
     1021 ggtctacagg catataccga actgaaaaac ttcctgtttt tcttgttgtc gcgaatgatt
     1081 gatagccccc ttaaaacgct tgaatcggga gaaggcattc tgtagcaact tgaaagcgga
     1141 atatctgagg agagaaccat gagagcggtt gatattatca tgaagaaaag agggaaccca
     1201 acagctccgg ccggttcaag cctttccagg gaagaaattg cctttttaat ctccgggtat
     1261 gtacaaggtg aaattccgga ttatcaaatc tcggcctggc tcatgtctgt gtactttaac
     1321 gaatgaattt tgttgaaact ggatacctta ccgatgtgat gcttacatca ggatcggtaa
     1381 ttgacctttc aggtcttacc ggcccgtttg tcgacaaaca ctcaaccggc ggagttggag
     1441 acaagatatc cctacccctt gctcccatag tagcggctgc cggggtatgc gtcccgatga
     1501 tgagcggcag agcgctggga cataccgggg gaacgcttga taaactagaa tccataaccg
     1561 gctataaaac ctccctttcg gtccgtgagt tccgtaacat cctttcaaaa acaggctttg
     1621 ccatgaccgg ccaaacgaaa gaaatcgttc cagctgaccg cctgctctac gcgctcaggg
     1681 atgttaccgg caccgttgag tcagtgcccc ttattaccgc aagcattctc tccaaaaaag
     1741 tagccgaagg agccgatgcc ctggtgtttg atgtaaaatg cggatcggga gcctttatga
     1801 agaaccttgc agatgcccag aagctcgcgg acagtcttgc cggtaccgga aaggcaatgg
     1861 ggaaaaaagt agtagccctg attaccgcaa tggatgagcc gcttggctat aaaattggta
     1921 actttttgga aattgaagaa tccatggacg ttcttgaggg acacgggccc gaagatgtga
     1981 ccgaattaac cctgctttgc ggagcctgga tgacggtatt ggcacataag gcttcctccg
     2041 tcgatgaagg aaaagatctt tgtagacaag cgatacactc cggcaaggca atggaattgt
     2101 ttttagaaaa cgtacgtctc cagggaggag atgttccccg attactggca gacagggaga
     2161 acagacggtc caagtatacc acgacgattc gtgcagaaca aacaggagcg attgcatcga
     2221 ttgacgcctt tacaaccggc cttgccggtg tatatctggg tgtaggacgc aacaaaacgg
     2281 atgaacaggt gtgcccggac gcgggtatta tcctgaaata taaaaaaggc gatcgggtta
     2341 atgctggaga cgttattatg gaggtatacg gtaaagacga agcgtgtctg g
//
LOCUS       DAOQXQ010000231         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49770_length_2390_cov_2.631692, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000231 DAOQXQ010000000
VERSION     DAOQXQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49770_length_2390_cov_2.631692"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKH81_04635"
     CDS             <1..378
                     /locus_tag="PKH81_04635"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ97356.1"
                     /translation="NVRYSSDYTPRLTGEETIQIENESVHCLVLELNAMRKGCTYNRM
                     VLWVDREGFFPVKADCYALSGKLVKTVYYSGRKHMGGHHIVTRVSLYDRVQENSHTVI
                     EFLEMVEEEISENLFNKEFLLRM"
     gene            389..1540
                     /locus_tag="PKH81_04640"
     CDS             389..1540
                     /locus_tag="PKH81_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97357.1"
                     /translation="MIHQLRFVILIFLYALLFITTIFAEYTMGPVKVSASAESIQRLL
                     HYDTPLNPDNVLNAEEGYNGFCRYTLALDLSGESASFFLADYGSFRADDNPDGYNCLA
                     ELFTSLRKGSLFLDLGKKRINASPSYFISPINFVLDDYRGYELRFSEGRIMINLEYFS
                     SIGFIGLSFLPEVDVPSRIERYLCSSQSRQCFTRYEISCDAGTLGFAASFDDRWRLGG
                     TYSRTIGQYTEFHTECVWDEKDKIQDVIGFTVHLSKASIIAEYYYNQSGYSFSEWEEK
                     IDEYKNACDVYQKMISYRDLVSNQGRNGRHYLMFRLSNPTTDNWEASITTLMNLQDLG
                     TFVVPKIAYSGWENLTVSMNVAFPVGPTWTEFALYGQAWTAELSMELWL"
     gene            1567..2310
                     /locus_tag="PKH81_04645"
     CDS             1567..2310
                     /locus_tag="PKH81_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97358.1"
                     /translation="MKTKTLLMLCCLLVSICFAQATDMEECFALNEYFNKPVPEVHQK
                     IAEYQNRLKTDKSDYYAHLAIAILYSALSSPMENPEEGASEKIVEHSKAFEKKEKNNV
                     LAMTYYGLGCSLVSRDSRNPFTQLSMVKRAIRTFDTAVEISDGHDLQWFVRYMRGNFY
                     INLPGSFKKRPAAEQDFEFISSYYTAHPEIEGYMCNAYFYLGEIEKTRGNLETAVSFW
                     KHSVSINERLALNAREGEQSAERLTVFAD"
BASE COUNT          677 a          447 c          546 g          720 t
ORIGIN      
        1 aatgtacgtt attcgtcgga ttatactcca cgccttaccg gggaggaaac gattcaaatt
       61 gaaaatgaat ctgtacactg tctggtttta gaattaaacg ccatgcgcaa agggtgtacc
      121 tataatcgaa tggttttatg ggtcgatagg gaaggattct ttccggttaa agctgattgt
      181 tatgcattga gcggtaagct ggtaaaaacc gtttactact cgggtagaaa gcacatggga
      241 ggtcatcata ttgtaaccag ggtaagcttg tacgatcgag tgcaagaaaa cagtcatacg
      301 gttatcgaat ttcttgaaat ggtagaagaa gagattagcg aaaacctttt caataaagaa
      361 ttccttttac gcatgtaaga gcatttgtat gatacatcaa ttacgatttg taatacttat
      421 ttttttgtat gcattgctct tcatcacaac catttttgcc gagtacacta tgggccctgt
      481 taaagtttct gcaagcgcag aatcaataca acgtttactt cattatgaca ctcccttgaa
      541 tccggataac gttttaaatg cagaagaagg ctataatggg ttttgccggt atacattagc
      601 tcttgatctg tccggagaga gtgcctcttt ttttcttgcg gattatggta gtttccgtgc
      661 agacgataat cctgacgggt ataattgcct tgcagaactt tttacttcat tgcggaaagg
      721 aagcctcttt cttgatcttg gcaagaagcg gataaatgct tcgccttcgt attttatttc
      781 tcccattaat tttgtgcttg atgattatcg cgggtatgaa ctcagatttt ctgaaggcag
      841 gataatgatt aatctggaat acttttcctc tattggtttt attggattat cttttcttcc
      901 cgaagtagat gtgccttccc gtattgagcg atatctatgc tcttcgcaaa gtcgacaatg
      961 ttttacccgt tacgagattt cctgcgatgc gggtacactc ggatttgccg catcatttga
     1021 tgatcgctgg cgtcttggcg gcacctactc gcgtacgata ggacaataca cagagtttca
     1081 tactgaatgt gtatgggacg aaaaggacaa gatacaagat gttatcgggt ttaccgttca
     1141 tctttccaaa gcctctatta tcgcagagta ctattataac cagtctggat atagtttctc
     1201 tgagtgggaa gaaaagattg atgaatacaa aaatgcatgc gacgtttatc aaaaaatgat
     1261 ttcgtatcgg gatctggtga gtaatcaggg aagaaacggg cggcattatc tgatgttcag
     1321 gctgtctaat cccacaactg ataattggga ggcgtcgata acgaccttga tgaatctaca
     1381 ggatcttgga acctttgtag ttccaaagat cgcttattca ggttgggaaa atttaactgt
     1441 atcaatgaat gtggccttcc ccgtcgggcc tacatggact gaatttgcat tgtatggaca
     1501 ggcatggaca gcagagcttt ccatggagct atggctataa gaaagactgg aatataagga
     1561 gttattatga agacaaagac tttattgatg ctgtgctgtt tgttggtttc aatctgcttt
     1621 gcgcaggcaa cggatatgga agaatgtttt gcgcttaatg aatattttaa taaaccggta
     1681 ccagaagtgc atcaaaagat tgcggagtat cagaacagat taaaaacgga caagagtgat
     1741 tactatgcac atcttgcaat agcgatatta tattcagcgc tttcctctcc gatggagaat
     1801 cccgaagaag gtgcgtcaga aaagattgtg gagcatagta aggcttttga aaaaaaagaa
     1861 aaaaacaacg ttttggcgat gacgtattac gggcttggtt gctctttggt aagcagggat
     1921 tcacgaaatc cttttacaca actttccatg gttaaaaggg ccataagaac atttgatacc
     1981 gcagttgaaa tttctgacgg tcatgatctc caatggttcg ttcgttacat gcgtgggaac
     2041 ttttatatca acctaccggg atcctttaaa aaacgtccgg cggcagaaca agatttcgag
     2101 tttatcagtt catactatac cgctcatccg gaaatagaag gctatatgtg taacgcctat
     2161 ttctatttag gcgagataga aaaaacccgc ggaaatcttg agacggccgt cagtttttgg
     2221 aaacattcgg tttctataaa tgaacgtctt gctcttaatg cccgggaggg agagcagtct
     2281 gccgagcgac taacggtttt tgcggactaa gtcctaatac gaaaaggctc cgcaatcggc
     2341 cgtgtcgctc aattgatatc ttttcccggc taaatctacg gcgagcgcct
//
LOCUS       DAOQXQ010000232         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49960_length_2384_cov_2.567196, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000232 DAOQXQ010000000
VERSION     DAOQXQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49960_length_2384_cov_2.567196"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..496)
                     /gene="def"
                     /locus_tag="PKH81_04650"
     CDS             complement(2..496)
                     /gene="def"
                     /locus_tag="PKH81_04650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ97359.1"
                     /translation="MEVITLGNETLRIKAAPVPEVTDEIRSLIREMFIVMEKADGVGL
                     AAPQIDLSIRLFVLKADDGIERAFINPQIIQTSPETCSYEEGCLSVPKVWEKVIRPEK
                     ITVQALNERGRPFTLEADGLLARIIQHEYDHLDGILFIDRIDPAKKAKAEEKFLKKAS
                     KKDA"
     gene            594..1163
                     /locus_tag="PKH81_04655"
     CDS             594..1163
                     /locus_tag="PKH81_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97360.1"
                     /translation="MQSDIFEKDKSMPDVVRKAVVEVLPKFISTSSDSVALSSAVSLA
                     LAFGDCGEFKAIVTGKDMVRDAAEKKLVNGFQKNIELLVQKTWVEKADESLKEEMLFR
                     INTLCKNLSRYDYHTSLSEFLPVLRDVVYLLFGSLVKSDTFLEYAVRVDPDFGFFWYY
                     INSLPPHQQWSEEKCRAAVLLGICFLANF"
     gene            1174..>2384
                     /locus_tag="PKH81_04660"
     CDS             1174..>2384
                     /locus_tag="PKH81_04660"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNQ97361.1"
                     /translation="MNLDAVCQTLRLASARLAMCTAERKNKALRGVASALDANRPEIL
                     EANACDVERARASGMSESLIDRLSLSHKSLDEIIRALHSLADQTDPIGQVVSGWTVPN
                     GPEITQVRVPLGVAAVIYESRPNVTVDAFALAYKSANAVLLRGSSSAIESNRVIVRVI
                     KEGLAASGGESDAVALCEPPQEGDKYADVDWILTAVGKIDIALPRGGSALIKRVVETA
                     RVPVIETGSGVCHIYVDESADLSMALDIIVNSKIQKPAACNSLECLLVHASRAKELLP
                     LVAKAFIPYEQKTGRAGGVEFHTDERSLALLSGCAQSVKPAVEADWGYEFLDYIMAVR
                     VVESLDEAIEYINAHSTKHSESIITASRDNARKFQKLLDSACVYVNASIRFTDGGEFG
                     MGAELGISTQKL"
BASE COUNT          560 a          575 c          624 g          625 t
ORIGIN      
        1 cctacgcgtc ctttttagag gcttttttca agaatttttc ctcggccttt gcttttttag
       61 ccggatcgat acggtctata aacagaatgc cgtcaagatg gtcatattca tgctggataa
      121 ttcgcgccaa tagcccgtca gcctccaggg taaagggccg tcctcgttcg ttaagggctt
      181 gaacagtgat tttttccggc ctgattacct tttcccatac cttgggcaca ctgaggcagc
      241 cttcttcgta ggagcaggtt tccggagatg tttggataat ctgaggattt ataaaggcgc
      301 gctcgattcc atcatcagct ttaagtacaa agagtctgat gctcaaatca atttgagggg
      361 cggcgagtcc tactccatca gctttttcca tgactataaa catttccctg atcagggacc
      421 gaatttcatc ggttacctcg ggaacggggg ctgctttgat acggagggtt tcattaccca
      481 gagtgattac ttccatagtg taaaaatacc gttttgagat tcaatggtca atgcggcgtt
      541 tacattgttg tccatggcct tgacaaaagg gggataaaaa tccattttta ggtatgcaaa
      601 gcgacatctt cgagaaagac aaatccatgc ccgacgtagt aagaaaggcc gtggtggaag
      661 tccttcccaa gttcatcagc acttcaagcg actccgttgc cctttcatcc gccgtttctc
      721 tggctttagc ctttggggac tgtggtgaat ttaaggcgat cgtaaccggc aaggatatgg
      781 tacgggatgc ggcagagaaa aagctggtta atgggttcca aaaaaacata gagcttctgg
      841 ttcaaaagac atgggttgaa aaagcggacg aatcccttaa agaggagatg ctgttccgca
      901 tcaatacact gtgcaagaat ctgtcccgct acgactatca tacctcgctg tccgagtttc
      961 ttccggtgtt gagagatgtg gtgtacctcc tgtttgggtc cttggttaaa agcgacacct
     1021 tccttgagta cgccgtccgt gttgaccccg attttggttt tttctggtat tatataaaca
     1081 gcctgcctcc ccaccagcag tggtccgagg agaaatgccg ggctgctgtt cttttgggaa
     1141 tctgtttcct cgccaatttt taggagaact tccatgaacc tcgacgccgt gtgtcagacg
     1201 ctgagattgg cctccgctcg tcttgccatg tgcacggccg agaggaagaa taaagccctt
     1261 agaggcgtag cttctgcgct ggacgctaat cgcccggaaa ttttggaagc aaatgcctgc
     1321 gatgttgagc gcgctcgcgc ttcgggtatg agcgaaagtt taatcgatcg tctatccctg
     1381 agccacaaat ctctggacga gattatcaga gccctgcatt cccttgctga tcaaacagat
     1441 cctataggac aggtagtttc cgggtggacg gttcctaatg gccctgaaat tacccaggtg
     1501 cgcgttcctt tgggagttgc cgctgttatt tatgaatccc gcccgaatgt aacggtagac
     1561 gcctttgccc ttgcctataa aagcgcgaac gcggtgttgc ttcgcggaag ttcttcggca
     1621 atcgagtcca atcgggtaat tgtgcgcgtc attaaagagg gtcttgctgc ctccggtgga
     1681 gaaagcgatg cggtagcctt gtgtgaacct ccccaagaag gcgataagta tgccgatgtt
     1741 gactggatac tgaccgcagt gggaaagatc gatatagccc tgcctcgtgg cggctctgcc
     1801 ctgatcaagc gtgtggttga aaccgctcgt gtacccgtaa tagaaaccgg ctccggtgta
     1861 tgccatatct atgtggacga aagtgccgac ctttccatgg ctctggatat cattgttaat
     1921 tcaaagatac aaaaacccgc cgcgtgtaat tcgcttgaat gtctgttggt gcacgcttca
     1981 cgggccaagg agctattgcc tctggtggca aaggccttta taccctacga gcaaaaaacc
     2041 ggacgagcgg ggggcgttga atttcatacc gacgagcgct cccttgcgct tctctctggc
     2101 tgtgcccaat cggtaaagcc cgcggtggaa gccgactggg gatatgagtt cctggattat
     2161 ataatggccg tgcgggtagt agagagcctg gacgaggcta tcgagtatat caacgcgcat
     2221 agcaccaaac attcagagtc aatcataacc gcaagccggg ataacgcacg gaagttccaa
     2281 aagcttctgg attctgcctg tgtatacgta aacgcctcca tcaggtttac cgatggaggg
     2341 gagttcggca tgggagccga gctcggcatc agtactcaaa agct
//
LOCUS       DAOQXQ010000233         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_49982_length_2383_cov_2.930842, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000233 DAOQXQ010000000
VERSION     DAOQXQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49982_length_2383_cov_2.930842"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKH81_04665"
     CDS             complement(<1..435)
                     /locus_tag="PKH81_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNQ97362.1"
                     /translation="MQIASTIFQIIGSLGLLLYGMKLMSDGIQKSAGDSLHRILNFMT
                     GNRLSAVLTGVGITAIVQSSSATTVMTVSFVNAGLLSLQQAIGVIFGANIGTTITAWI
                     VSLFGFKFKLTVFAIPAFGIGYFINFFKRLHREGLGEAIMGFG"
     gene            502..1239
                     /locus_tag="PKH81_04670"
     CDS             502..1239
                     /locus_tag="PKH81_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97363.1"
                     /translation="MKLVATFIALACSLCLFLSCTARGEPAFFPHTEGLSTPQKASIG
                     SPRRHHQGYRVLLVAGPQYIALTGIDEELTKEFGAESGGGMYSRVERLRDLSYLPDRS
                     IILTIGAPEGTVRDIVRLHDTFPTLRVATLMPQEDSLAVEEISDLVVNITQSDEVLAQ
                     EGSEQLLESRELSLLLLASALYLEQEGATGLAETRMPQVLDIVRKRVKQKDAGKNWHF
                     TAWRDPENSLKSKNHLIFVPAGGQGEP"
     gene            1236..1889
                     /locus_tag="PKH81_04675"
     CDS             1236..1889
                     /locus_tag="PKH81_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNQ97364.1"
                     /translation="MNCLERPLPGILGGEADLDRLKRRDSSRLLVISDSHGHYQAVEQ
                     IIKKQGPLCDALLFAGDGIWDIVQYVENTFTSSRLRDALPPVVALVAGNGDGDEYRIT
                     LPSDAAPEEAPGLSLTVPDRLIVQASGYQVFLTHGHRYSVDVSLDILLASAHATACDI
                     AIYGHTHIPFTEEVSHLTALNPGSVSRPRGNSHPSCALLELDSTRTQPIVRFIHVKA"
     gene            complement(1915..>2383)
                     /locus_tag="PKH81_04680"
     CDS             complement(1915..>2383)
                     /locus_tag="PKH81_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNQ97365.1"
                     /translation="FNLFPHMSVMENITLAPIKVYSLSKKSAEERAYDLLDQVGLTDK
                     AHAHPAQLSGGQKQRIAIVRALAMEPKLLLFDEPTSALDPEMVGEVLQVMNNLAKRGM
                     TMAVVTHEMRFAREVASRVLFMDEGIIVEEGSAQKLFTAPENPRTREFLAKVL"
BASE COUNT          592 a          661 c          597 g          533 t
ORIGIN      
        1 tccaaagccc ataatcgcct cgccaagacc ttcccgatgg aggcgcttaa aaaagttgat
       61 aaaatagccg attccaaagg ccgggatggc aaagaccgta agcttaaact taaagccaaa
      121 gagcgataca atccatgcgg taatcgttgt accaatattg gcgccaaaga taaccccgat
      181 cgcctgctgc aggcttaaaa gccctgcatt aacaaacgag acggtcataa ccgtagtcgc
      241 gctggaagat tgaacaatcg cagtgatacc aacgccggta agcaccgcag aaaggcgatt
      301 gccggtcata aagttcaaaa tacggtggag agagtccccc gcgctttttt gaattccgtc
      361 gctcattaac ttcatcccgt acaggagaag cccaaggctg ccgattatct gaaagatggt
      421 ggaggcgatt tgcataaacc gacaatacca gaatagtatg cccttttcaa tgcccgaatt
      481 accggttatg atggacaaac gatgaaatta gtcgcgactt ttattgctct tgcctgttct
      541 ctttgcctct ttctttcgtg tactgcaaga ggggaacccg cattcttccc gcatacagaa
      601 ggattatcga caccccaaaa ggcatcgatt ggctctcccc gccgccatca tcagggctat
      661 cgggtacttc ttgtagcggg cccgcaatac atagccctga ccggaataga cgaagaatta
      721 accaaagaat tcggagcaga gtcagggggc ggcatgtaca gccgcgttga acggcttcgc
      781 gatctctcat acctccccga tcgctcgatc atccttacca taggagctcc cgaaggcacc
      841 gtacgagaca tcgttcgtct acacgacacc ttcccgaccc tccgtgttgc aaccctgatg
      901 ccccaggaag actcacttgc cgtggaagaa atctccgacc tggtagtaaa tataacgcag
      961 agcgacgagg tcctggccca ggaaggcagt gaacagctgt tagaatccag ggaactgtcc
     1021 cttcttttat tggcctcggc cctgtatctg gagcaggaag gagctacggg ccttgcagaa
     1081 acgcgaatgc cgcaggtgct cgacattgta cgaaagcgcg ttaaacaaaa ggatgcggga
     1141 aaaaactggc attttaccgc ctggcgagat ccggaaaatt cccttaaatc aaagaatcac
     1201 ctgatatttg tacctgctgg aggacaaggc gagccatgaa ttgccttgag cgaccgctac
     1261 cgggaatact ggggggcgag gccgatctgg atcgccttaa gcgacgcgac agttcccggc
     1321 tgcttgttat ctcggattcc catggacact atcaggcggt agaacagata atcaaaaaac
     1381 aaggaccctt gtgcgatgcc ctcctttttg ccggagacgg catttgggat atcgttcaat
     1441 atgtggaaaa tacctttact tccagccggc tcagggatgc ccttcccccg gtagtagcgc
     1501 tggtagcggg taacggggac ggcgatgaat accgcataac cctgccttcc gatgccgctc
     1561 cggaggaggc acccggcctt tcgcttaccg tacccgaccg gcttatcgtt caagcctccg
     1621 gctaccaggt gtttttaacg catggacacc ggtactcggt ggacgtttcc ctggatatcc
     1681 tgcttgcttc ggctcatgca acggcctgcg atatagccat ctatggacat acccacatac
     1741 cctttaccga agaagtaagt cacttaaccg ccttgaaccc cggcagtgtg tcccgaccac
     1801 ggggtaactc ccacccttcg tgcgccctgc tggagttgga tagcacccga acacagccga
     1861 tagtccgctt tattcacgtt aaagcgtaaa agctaccaac aatcctgcga gcacttataa
     1921 cacctttgca aggaactcgc gtgtacgggg attctccggc gcagtaaaca gcttctgggc
     1981 actcccttct tccacgatta tcccttcatc cataaaaagc acccggcttg ccacttcccg
     2041 ggcaaaacgc atttcgtggg ttaccacggc catggtcatg cctcgtttgg caagattatt
     2101 cattacctgc aggacttcgc caaccatttc cgggtccagt gcgctcgtgg gctcatcaaa
     2161 aaggagcagt ttcggctcca tggccagggc ccggactata gcgattcgct gtttttgccc
     2221 gccggaaagc tgggctgggt gggcatgggc cttgtcggta agaccgacct gatcgagtaa
     2281 gtcataagcc cgctcttcgg cgctcttttt ggaaagcgag taaaccttga tgggcgcaag
     2341 ggttatgttt tccataacgc tcatgtgggg aaagaggtta aaa
//
LOCUS       DAOQXQ010000234         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50035_length_2381_cov_3.503009, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000234 DAOQXQ010000000
VERSION     DAOQXQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50035_length_2381_cov_3.503009"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKH81_04685"
     CDS             <1..469
                     /locus_tag="PKH81_04685"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNQ97366.1"
                     /translation="DNFNKLSAGTEFENMPLEEVIKKATGGVFNNGAQVWNHTFYWNC
                     LNKPDTTKPSDKLLKAIENQFGSLDNFKEQFSNAAVTLFGSGWAWLCKDEFGKLYINQ
                     TSNAENPIKNGLTPIMTIDVWEHAYYLDKQNRRPDYVKDFWHILNWDFVSKLF"
     gene            606..860
                     /locus_tag="PKH81_04690"
     CDS             606..860
                     /locus_tag="PKH81_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97367.1"
                     /translation="MLFIGCAFVVSGFIQYGRFHVIYMAPAIVFSALGFLFLLFSTEV
                     ISVSFASVVLWWFPILLIPSLASLFIWIFRRGKADEADNE"
     gene            853..>2381
                     /locus_tag="PKH81_04695"
     CDS             853..>2381
                     /locus_tag="PKH81_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97368.1"
                     /translation="MNKRPAVHPAAACIALVCFLFFSCASSPAAGDEEATDSSTTVPQ
                     ALTAPGTPLAVPSKDQLKKNALDGQTLALLQTGSPQSIREAVRQINADSRGMTDQNRS
                     ALFLAAELMRSLYPYETIDWPIPAIADGDPYAAIIRSAKMGTYEYSRDSDDFFALVLP
                     SLILPLTSAQGSWYQDAQASLLRASRQNDQSVLPPLFLAMLSERQNKTDIALSFYRKA
                     WDIDDSCYPAGMALSRLFIAQGDGASALSVAQTLSVRYPSQIAITRLCAESAFVLRDW
                     AMADSYILAVLKAEPGNTGYLLLRARILIERKEYLKANSLLDAFATQSRTDKTYLLLR
                     SRVSREWNRNPVAATAFLQDALRLYPEDIDVLLSGAEVAYLTGQTVHQKTGRELVRMV
                     LAREKENRVALTLLLDDYLASGEWPLAVTLADQLVALDGSTESRLRLARALLGSGSVP
                     RAISQTSALYAANPANESVVSLHLRALVASGNLEGARIVISERMPSAASSLKSTLHYY
                     E"
BASE COUNT          553 a          572 c          545 g          711 t
ORIGIN      
        1 agataatttt aacaaattat cggctggcac tgaattcgaa aacatgccat tagaagaagt
       61 tatcaaaaaa gctactggtg gagtttttaa taatggagca caagtatgga atcacacatt
      121 ctattggaat tgtttaaata aaccagacac tactaaacct tctgataaat tactaaaagc
      181 tatagaaaat caattcggta gtttagataa tttcaaggaa cagttctcaa atgctgcagt
      241 tacacttttc ggctctggat gggcatggct ttgcaaagat gaatttggca agctatacat
      301 caatcaaaca tctaatgcag aaaatcctat aaaaaatgga ttaacgccaa ttatgacaat
      361 agatgtgtgg gagcatgctt attatttaga taaacaaaat agacgcccag actatgtgaa
      421 agatttttgg cacattctaa attgggactt tgtaagtaaa ttattttaat tgtctcttta
      481 tcttattatt tggcagaggt acctcatagg gtgcctctgt ttttttattt ttaacgggta
      541 ttctcttttt tattcttgat tttcagcttc ttcatgttcc ttcccgggca gtatggcccc
      601 tggagatgtt gtttatcggg tgtgcctttg tcgtttccgg ttttattcaa tatggtcgct
      661 tccatgtcat ctacatggct ccggcaatcg tattcagcgc gttgggattt ctttttttgc
      721 tgttttctac agaagtaatt tctgtatcct ttgcctctgt ggtgttgtgg tggtttccca
      781 tcttgcttat tccctctctg gcatccctgt ttatttggat attccggcgc ggcaaggctg
      841 atgaggcgga taatgaataa gcgtcccgct gtacacccgg cggccgcttg tatcgcttta
      901 gtgtgttttt tattcttttc ttgtgcctca tcccctgcag caggggatga agaagcgacc
      961 gattcatcca ctaccgttcc acaggcatta acggcaccgg gaacccctct ggctgttccc
     1021 tctaaagacc agcttaaaaa aaatgcactt gatgggcaaa ccctcgcctt attacaaacc
     1081 ggttcacctc agtcgattcg ggaagcggtc aggcagatta acgcagacag tcggggtatg
     1141 accgatcaaa atcgcagtgc cctctttctt gctgccgagt taatgaggtc tttgtatccc
     1201 tatgagacga tagattggcc aattccagct attgccgacg gggatccgta tgcggctatt
     1261 attcgttctg ccaagatggg tacctatgag tattcgcggg attctgacga tttttttgcc
     1321 ttggtgcttc cctctcttat tctcccgctt acctcagcgc agggctcatg gtaccaagat
     1381 gcacaggcct cactactccg tgcctcccgc caaaacgatc aatcagtcct gcctccgctt
     1441 tttttggcaa tgctgagcga acgtcagaat aaaaccgata ttgcgctgtc cttctataga
     1501 aaagcgtggg atatcgatga ctcctgctat cctgccggaa tggccctttc gcggctgttt
     1561 atcgcgcagg gggacggcgc atcggccctt tctgtcgctc agaccctttc tgttcgctat
     1621 ccctcccaga tagcaattac tcgtttgtgc gctgagtctg ccttcgttct tcgtgactgg
     1681 gctatggcgg acagctacat ccttgccgtg ttaaaggctg aaccggggaa taccgggtat
     1741 ctcttgctca gggccaggat tcttattgag cgaaaagagt atcttaaggc aaattccctg
     1801 ttggacgcgt ttgctaccca aagtcgcact gataaaacat accttttgtt gcgctcccgt
     1861 gtttcccggg aatggaatcg aaatcccgta gccgcaactg cgtttttgca ggacgccctc
     1921 aggttgtatc ccgaagatat agatgtcctg ttatccgggg cagaggtggc ctatttgacc
     1981 ggtcaaacgg tacaccaaaa aacaggacgc gagctggtac gtatggttct tgcacgcgag
     2041 aaagagaacc gcgtggcctt gaccctttta ttggacgact accttgcctc cggggaatgg
     2101 ccgcttgcgg ttactcttgc cgatcaattg gtagcccttg acggaagcac cgaatctcgc
     2161 ctgcggctcg ctcgagctct tttaggttct ggctctgtcc ctcgggcgat tagtcaaacc
     2221 tcagccttat atgccgcaaa ccctgccaac gagtcggtgg tatcccttca tttacgagcc
     2281 ctggtggcaa gtggtaattt ggaaggagcc cggattgtaa tctccgaacg catgccatct
     2341 gccgcatctt ccctaaagag tacccttcat tattatgaaa g
//
LOCUS       DAOQXQ010000235         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50337_length_2372_cov_2.567113, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000235 DAOQXQ010000000
VERSION     DAOQXQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50337_length_2372_cov_2.567113"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKH81_04700"
     CDS             <1..1213
                     /locus_tag="PKH81_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNQ97369.1"
                     /translation="TVENTGINAIVNGSEEVPNLVDPSKVDVVPGWQRLMMMAIGFLI
                     IYLGAAKGFEPLLLIPIGFGTLFVNIPGGGMYAEHSGMLKIIYDAGVGNEFFPMLIFM
                     GIGAMTDFGPLIANPKTAILGGAAQLGVFITLFGVAVLNLLPGIEYNMFEAAAIAIIG
                     GADGPTSIYLSGKLAPHMMAVIAVAAYSYMALVPMIQPPIMKMLTSKKERSIRMKQLR
                     HVSQAEKILFPISLLILSILLIPTAAPLIGMLAFGNFVKVIGVVERISKTMENELLNI
                     VSILLSLGVGSQMTPEKIMNPNAVGIILLGLLAFCVATAGGVMMAKFMNLFLKEKINP
                     LIGSAGVSAVPMAARVSNKVGLEYDPSNFLIMHAMGPNVAGVIGTAIAAGVFIATYGE
                     AAAKVLTSVVH"
     gene            1287..>2372
                     /locus_tag="PKH81_04705"
     CDS             1287..>2372
                     /locus_tag="PKH81_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ97370.1"
                     /translation="MRCTIQFLPIICVLLVVSCDTSVVRYTSAKHIQEYTSLEWARLE
                     AKSFLDQLTLREKASQVLMTGIDGKDSFPGHLYRHFNNLIPGFILLLGYNVTTSPQGV
                     KAYIDSCQHAFSSLGSRLPVVFSLDHEGGTVFRTALVTSNLPSQAFIAQNATITQAEK
                     VYFASGQQLRALGITLNLAPVSEIPIDGDSSFISSRIFSPDVQQSTRYSLAAIQSYAR
                     AGVLCAPKHFPVSPKFDPHDSPGVVSISPEDMNSHFIVPFQSLISNGVPALLVSHAVV
                     TAIDATLPFCLSSKGVTGYLRNTLGFNGLVITDDISMKALTYNEARTSRDNALLALRA
                     GCDVVMTSDTDIRRIVAAIEQEASKDPS"
BASE COUNT          556 a          523 c          522 g          771 t
ORIGIN      
        1 taccgttgaa aataccggta tcaacgcaat tgttaatgga tcagaagagg ttcctaacct
       61 tgtagatcct tcaaaagttg atgttgtccc cggttggcag cgattgatga tgatggccat
      121 aggttttctt atcatctacc tgggagcagc aaagggcttt gagccattgc ttcttattcc
      181 gattggattt ggtaccttat tcgtaaacat acccggaggc gggatgtatg cggaacactc
      241 tggtatgctc aagattattt atgacgccgg tgttggaaat gagtttttcc cgatgctcat
      301 ctttatggga atcggtgcga tgactgattt tggtccgctt attgccaatc caaaaacagc
      361 cattcttgga ggtgctgcac aattgggtgt atttattact ctctttggtg ttgcggttct
      421 taatcttctt cccggaattg agtacaatat gttcgaagct gccgctatcg ccatcatcgg
      481 tggtgcagac ggtcctactt cgatctatct ttccggaaaa ctagctccac atatgatggc
      541 cgttatcgcg gtcgcggcct attcgtatat ggcccttgta ccaatgattc aaccccccat
      601 catgaagatg ctcacaagca agaaggagcg atccatacga atgaagcaac ttcgccatgt
      661 atcacaggcg gagaagattc ttttccccat ctccttactc atcctttcca ttctgcttat
      721 tcctacagca gccccgctca ttggtatgct ggcgtttgga aactttgtta aggtaattgg
      781 tgtagtagag cgcatttcca aaaccatgga gaatgagtta ctgaatatcg tatcgatcct
      841 gttgtctctt ggtgttggct ctcagatgac tccggaaaag atcatgaatc caaatgctgt
      901 aggtattatc cttcttggac tcttggcttt ctgtgtcgca accgccggtg gcgttatgat
      961 ggctaaattt atgaatttgt tccttaagga aaagataaac ccgctgattg gttcagctgg
     1021 tgtatccgca gttcctatgg ctgcgcgtgt atcaaataag gtcggtcttg agtatgatcc
     1081 ttctaacttc cttattatgc acgccatggg acctaacgtt gctggtgtta tcggtactgc
     1141 aattgcagct ggtgtgttca ttgcaacata tggggaagca gcagcaaagg tattaacctc
     1201 tgtggttcac taataacgca gaacctgttt tctgttaatg tatgggccat ccttgcaaaa
     1261 gggtggcctt ttttttatac tcgcttatgc gttgtacgat acagttctta cccatcatat
     1321 gtgttctact tgttgtatct tgcgatacat ctgttgttcg ttatacatca gcaaagcaca
     1381 ttcaagaata tacttcctta gaatgggctc ggcttgaagc aaaatcattt cttgatcaat
     1441 taacgctacg cgagaaggcc tcccaggtct taatgaccgg tattgatggc aaagacagtt
     1501 ttccaggtca tttatatcgt cattttaaca acctgattcc cggctttatt cttttgctgg
     1561 gctataatgt gactacaagt cctcaagggg ttaaagcgta tattgattca tgccaacatg
     1621 ccttttcgtc tcttggctct cgccttccgg ttgttttttc tttggaccat gaagggggga
     1681 cggtatttcg tactgcattg gtaacaagta atcttccttc ccaggctttt attgcgcaaa
     1741 atgcaaccat tactcaagct gaaaaagtat actttgcctc tggacagcaa ttacgtgctt
     1801 tgggaattac cttaaatctt gcacctgttt cagagattcc tattgatgga gattcctcgt
     1861 ttatcagttc tcgcatcttt agtccggatg tacaacaatc tacccggtat tctcttgctg
     1921 caattcaatc gtatgcccgt gcaggtgttc tctgtgcccc taaacacttt ccggttagtc
     1981 caaaatttga tccgcatgat tcacctggtg tagtttctat ttctcctgaa gatatgaaca
     2041 gtcattttat tgtacctttc cagtcgttga ttagtaatgg tgttcctgcc ttgttggttt
     2101 ctcatgcagt ggtcaccgct attgatgcaa cgcttccttt ttgtctctcg tcaaaagggg
     2161 taacagggta tttacgaaat accttgggat ttaatgggtt ggtaattacc gatgatattt
     2221 ccatgaaggc tcttacttat aacgaagcgc gcacctcaag agataacgcc ttactcgccc
     2281 ttcgcgcagg ctgtgatgtg gtgatgacca gtgatactga tattcgccgt attgttgcag
     2341 ctattgaaca agaggcgagc aaagatcctt ct
//
LOCUS       DAOQXQ010000236         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50542_length_2366_cov_2.548680, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000236 DAOQXQ010000000
VERSION     DAOQXQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50542_length_2366_cov_2.548680"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..716
                     /locus_tag="PKH81_04710"
     CDS             186..716
                     /locus_tag="PKH81_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97371.1"
                     /translation="MTQKEHLTIDCLFLHDSLMAILEGEQRILRGIVQGDLPAHFLPP
                     LCSLADHIPIETLSLCEVEEKDGWLFRPVTITPPPATIARYDPHGGMFPPPPHECGFL
                     LGYAGSYAQAALAAIQDRDELTTEKKVRELRVWYRARMQIWYDFENPVCLGARTSIET
                     LRWERSRPLTSGAKPQ"
     gene            complement(691..1347)
                     /locus_tag="PKH81_04715"
     CDS             complement(691..1347)
                     /locus_tag="PKH81_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNQ97372.1"
                     /translation="MSRSEEKETIADRFASIRTDAGLSRKAFAESLSIHPVVAGDIEL
                     GKREPSREVLVKLVQTYGVDASWLLTGRSAVAPSASAGEGMVAIACIDQEAAAGRGAE
                     IADYPESKTITVPRSFIGSRSPSHVRSVPVRGDSMIEAGLDDGDVVFFSVDERSGDGI
                     HVVSVGNQLLVKRVEFDHTSRTVRLISENPRYGPRVLEGADLELFSIEGRVIGVLHRM
                     "
     gene            1441..1791
                     /locus_tag="PKH81_04720"
     CDS             1441..1791
                     /locus_tag="PKH81_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNQ97373.1"
                     /translation="MATETGFPSPARGYEARTIDLNDLLVTNAPATYFMRMRGSHLVY
                     RGILPEDLLAVDRSRPPVPGCIVVFRQDDEFMCRELIRDRDSYVLADGTGNHLLVTEE
                     TEIFGTVTGIVRKL"
     gene            1788..>2366
                     /locus_tag="PKH81_04725"
     CDS             1788..>2366
                     /locus_tag="PKH81_04725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="binds processed UmuD protein to form functional DNA
                     pol V (UmuD'2UmuC); involved in translesion
                     polymerization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuC"
                     /protein_id="HNQ97374.1"
                     /translation="MIFHVDANSFYASCERLFRPDLADKPIVVLSNNDGIVIALNAEA
                     KALGVKRGDVWYKIAEACSQKGITAFSSNYTLYADISSRIVSIYNRLCPDVEVYSIDE
                     CFLYYPDWKCPDYEGIAWRIRTCVQREIGIPVSVGIAPTRTLAKIANKLAKKANGVYS
                     LAGGTDQSVDAVLAGLPAADVWGIGWSKAAHLQ"
BASE COUNT          594 a          640 c          596 g          536 t
ORIGIN      
        1 cagaataagg gcaaaataag gatcaagggt gtccgccgct gcagcctcgt ttagacaaat
       61 tgcctcgact ccttcccagg atgaaatgac cgaaacgagt gtgtctgcca gggtacgaac
      121 ctttggtttc atggtataat tcatgacacc atagtaccat ggaaatacat taatttggaa
      181 ctgtcatgac gcaaaaagag catctgacca tcgactgtct ttttctccac gactcactga
      241 tggccatcct ggaaggggaa caaagaatcc tgagaggcat cgtacaaggc gaccttcccg
      301 cacacttcct cccgcccctg tgctctcttg ccgaccatat tccaatagaa acgctttctc
      361 tgtgtgaagt cgaagaaaaa gatggctggc ttttccgacc ggtaacgatc accccgcctc
      421 ccgcgaccat tgcccgctat gatccccatg ggggaatgtt ccctcccccg ccacatgaat
      481 gcggctttct tcttggatac gcaggatcgt acgcacaggc agcccttgcc gcgatacaag
      541 atcgggatga gctgacaaca gaaaaaaaag tacgcgaatt gcgggtgtgg tatcgggcca
      601 gaatgcagat atggtatgac tttgaaaacc ctgtgtgcct tggagcgcga acgtcgattg
      661 aaacgctcag atgggagcga tcccggccgc ttacatccgg tgcaaaaccc caataacccg
      721 gccttcaatc gaaaacaatt ccaaatcggc accttcaagg actctaggtc cgtagcgggg
      781 attctcgctg atcaggcgaa ctgtccggga cgtatgatca aactcgaccc gttttacgag
      841 taactgattt ccaaccgaaa ccacatgaat accgtctccc gagcgctcgt caacagagaa
      901 aaaaaccaca tccccgtcat caagtcccgc ctcgatcatc gagtcgccgc gaacaggaac
      961 ggagcgcacg tgggaaggcg agcgagagcc aataaaagag cggggaactg ttatggtttt
     1021 tgactcagga tagtcggcta tctccgcccc tcgtcccgcc gcagcttcct gatcgataca
     1081 ggcgattgca accatacctt ccccggcact cgcacttggc gcgacagcag accttccagt
     1141 aaggagccat gaggcatcca caccataggt ttgaaccaat tttaccaaca cctcccgcga
     1201 aggctcccgt ttgccaagct caatatcacc ggcaactacc ggatgaatag aaagcgattc
     1261 ggcaaaggcc tttcgggaaa ggccagcgtc agtacgtatt gaagcaaacc tgtccgcgat
     1321 cgtttctttt tcttcagaac gactcatcaa tcctcccttg acaagcaagc attatgctta
     1381 tacgattagc atatcagtta ttttgcggta tgtcgagtgc ttccgcgata aagaggcaac
     1441 atggcaacag aaaccggatt tccctcgccc gctcggggct atgaggctcg aacaattgat
     1501 ctcaatgacc ttctggtcac caatgcaccg gcaacctatt ttatgcggat gcgtggtagc
     1561 catctggtgt accgggggat actgcccgag gacctgcttg ccgttgaccg ctcccgtcct
     1621 cccgtacccg gctgtattgt cgtgtttcgc caggatgacg agtttatgtg cagggagctt
     1681 atccgggacc gcgattcgta tgtccttgcc gacggaacgg gaaatcatct gctggttacg
     1741 gaagaaaccg agatattcgg cactgttacc ggtatagtga ggaagctatg attttccatg
     1801 tggacgcgaa cagcttctat gcttcgtgcg agcgcctttt tcgtccggat ttggcggaca
     1861 aaccgatcgt cgtcctgtcc aacaacgacg gaattgtaat cgcccttaac gcggaggcta
     1921 aggcccttgg cgtaaagcga ggggatgttt ggtacaagat tgccgaagca tgctcgcaaa
     1981 agggaataac cgcttttagt tcaaactata ccctctatgc ggatataagc tcgcgaattg
     2041 tcagtatcta taacaggctg tgtccggatg tcgaggtcta ttctattgac gaatgttttt
     2101 tatattaccc cgactggaaa tgcccggatt acgagggtat tgcctggcgc atacgtacct
     2161 gtgtacaaag ggaaatcggg atccccgtat cggtgggaat cgcaccaacg cgaacgctcg
     2221 caaaaatagc caataagctg gctaaaaagg caaacggggt gtattccctt gccggcggga
     2281 cagaccaatc agttgatgcg gttctcgcag gtctgcccgc agccgatgtg tggggtatcg
     2341 gttggtcaaa ggccgcccac ttacaa
//
LOCUS       DAOQXQ010000237         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50694_length_2362_cov_2.425661, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000237 DAOQXQ010000000
VERSION     DAOQXQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50694_length_2362_cov_2.425661"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..579)
                     /locus_tag="PKH81_04730"
     CDS             complement(1..579)
                     /locus_tag="PKH81_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNQ97375.1"
                     /translation="MSDLIKIFISAVLIENVVLIRFLALCPFIGMSSDVKKSTGMSGA
                     VLFVTVLATAVTWPLYVYVLAPLDLKFLQILTFILVIASLVQLVEFYLKKTAPALYSS
                     MGVYLALITTNCAILAVTFDVIAKGYTFVESLVYAVGVATGFALSLLLLAGIREKLKI
                     APIPSFLRGTPIMFIAAALLSMAFGGFAGLIK"
     gene            complement(576..1187)
                     /gene="rsxE"
                     /locus_tag="PKH81_04735"
     CDS             complement(576..1187)
                     /gene="rsxE"
                     /locus_tag="PKH81_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HNQ97376.1"
                     /translation="MKKYLSYFTNGILRENPLLMLMIGLCSALAVTTSLANGIGMGIA
                     MTFVLLMSEIIISAFRSLIPQSIRIPVFIIVIAAFTTIVDLVMKAFTPELSKAMGVFI
                     PLIVVNCIIMGRVESFSYKRTITESIADALGMGLGYTWVLCSISFIRELLGAGTILGM
                     EVLPESIRMGFFAQSPGGFFVFGLMIAVALAAKKALERKGGSR"
     gene            complement(1200..2318)
                     /locus_tag="PKH81_04740"
     CDS             complement(1200..2318)
                     /locus_tag="PKH81_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNQ97377.1"
                     /translation="MKTMLKLSFVMGVYAAVACLALAGVYLLTAPRIAAAENRAVNET
                     LSVIFPDADNFKDVSGSVTSPGGQIVFDGAYVAYTGSEVVGMVIRATGPTYKSSTLLV
                     GVDTKRTLKPVQFMANTDTPGLGTKTAEPAFSGQFTGKAIDDAFSLGDDLDNISGATI
                     SSRGVAEIVKIASWSAGDYLAREHGSLAGTGDGPKIVELAPMEAQKALEDIFPGSEFV
                     PIENLANTLERSIVFDRSWLVRSGDTVVGVAIEARGQTYKATTAIVGVTPDRILAGVR
                     ITETTDTKNYGWNMNDPAFYGLFTGMSADAPFLVRPTSADGDIDALSGATVSSLGFAN
                     IVKVASLEGARYLADNEGGKPAPASAQELVLNVIPDEQ"
BASE COUNT          689 a          623 c          565 g          485 t
ORIGIN      
        1 ttatttaata aggccggcaa agccgccaaa ggccatggac aataaggcgg cagcgataaa
       61 catgatcggg gttccccgca aaaaggaggg aatcggtgcg atcttgagct tctcgcgaat
      121 accagccaac aagagtaagg aaagggcaaa accggtagca acgccgactg cgtacaccag
      181 ggactccaca aaggtatagc cctttgcgat tacgtcaaag gttaccgcca aaatcgcgca
      241 gttagtggta atcagggcaa gatagacacc catggagcta tacaaggcgg gcgcagtctt
      301 cttaagataa aactctacca attgaaccag gctcgcgatt accagaataa acgtcagaat
      361 ctgtaaaaac tttagatcaa gcggcgcaag tacatacaca tacaagggcc aggtaaccgc
      421 cgtcgccaac accgtaacaa aaagaacggc tccgctcatg ccggtggatt ttttaacatc
      481 cgaagacatc ccgataaagg gacaaagggc caaaaaccgg atcaagacaa cgttttcgat
      541 caacacagca gatataaata tctttatcag gtcgctcatc gtgagcctcc cttgcgctca
      601 agcgcttttt ttgcggcaag cgccacggct atcattaacc cgaacacaaa gaatcccccc
      661 ggcgactggg caaaaaagcc catccggata gattcgggca aaacctccat cccaaggatg
      721 gttcccgcgc caagcaattc gcggataaag gaaatggaac agagaaccca ggtatagcca
      781 agacccatac caagggcatc ggcaatactt tcggtaatag tacgcttgta cgaaaaggat
      841 tccactcgtc ccatgatgat gcagttaacg acgatcaggg ggataaacac acccatcgcc
      901 ttggacaatt ccggggtaaa ggccttcatc actaaatcga cgattgtggt aaaagcggcg
      961 ataacaataa taaagaccgg gatacggatt gattgaggaa taagagagcg aaaggcgcta
     1021 atgataatct cgctcatcag tagaacaaag gtcatggcga ttcccatacc gatgccattc
     1081 gccaaactcg tcgttaccgc aagggccgag cacaaaccga tcatgagcat gagtagagga
     1141 ttttccctca agattccatt ggtaaaataa ctaagatact tcttcattgt catgcttcct
     1201 tattgctcat ccgggattac gttaagcacc agttcttgcg cagaagcggg tgcgggtttt
     1261 cccccttcat tatctgccag gtagcgagct ccctcaagag aagcaacctt tacgatattg
     1321 gcaaaaccaa gactagagac cgttgctccg gaaagagcgt caatgtcccc atcagcgctg
     1381 gtcggccgaa ccaaaaacgg cgcatcagcc gacataccgg taaacagacc ataaaaagct
     1441 ggatcgttca tgttccagcc gtaattcttg gtgtctgttg tctctgttat ccgcacaccg
     1501 gcaagaatcc ggtcaggggt aacacccaca atagcggtcg ttgccttata ggtttgaccg
     1561 cgggcctcta tagccacacc aacaaccgta tcgcccgatc ggacaagcca ggagcggtca
     1621 aatacaatag agcgctcaag ggtattcgca agattctcta tagggacaaa ctcggatccc
     1681 ggaaagatat cctcaagggc tttttgagct tccatggggg caagttcaac aatctttgga
     1741 ccatctccag ttcccgccaa agatccatgt tcgcgggcaa gatagtcccc ggcagaccag
     1801 gatgcgattt ttacaatctc ggcaacacca cgggaagaaa tcgtagcgcc agaaatgttg
     1861 tccaggtcat cgcccaggct aaaggcatcg tcaatcgcct tcccggtaaa ctggccggaa
     1921 aaagcaggct ctgcggtttt tgtacccaag cccggggtat cggtattcgc cataaactgc
     1981 acaggtttaa gagtgcgctt ggtatctacc ccaacaagga gggttgaact tttataggta
     2041 ggaccagttg cacgaataac catgccaact acctcactac ccgtataggc aacataggcg
     2101 ccatcaaaga caatctggcc gccaggactc gtcaccgagc cagagacatc cttaaaattg
     2161 tcggcgtcgg gaaagataac cgaaagggtc tcgttaaccg cacgattctc tgctgccgct
     2221 attcgcggcg ccgtaagcaa gtacactccg gcaagggcga ggcaggcaac tgccgcgtat
     2281 acacccatta caaaggacag tttgagcatg gttttcatga tgcgcgcttc tcctttgcgg
     2341 caagactaac cttgccgtat tt
//
LOCUS       DAOQXQ010000238         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50881_length_2356_cov_3.604520, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000238 DAOQXQ010000000
VERSION     DAOQXQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50881_length_2356_cov_3.604520"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="cheB"
                     /locus_tag="PKH81_04745"
     CDS             complement(<1..860)
                     /gene="cheB"
                     /locus_tag="PKH81_04745"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNQ97378.1"
                     /translation="MEKIKVLIVDDSAIVREILAQRLSRDPRIEIVGTAMDPYIARDK
                     LEKIDVDVLTLDIEMPRMDGLTFLRQLMEQFPKPVIVVSSLAKDANAAAMKALELGAV
                     EVVPKPGGPFSVEEVVPYLAERIVAASKANIARLKQSLPSPSSLLTPIKQTNRRRYLA
                     DLKTTNQLIAIGASTGGTIALEALFREWEDSFPPTLVVIHMPERFTATFASRLNDLSS
                     ARVKEAENGERLLSGTIYIAPGNYHMLLRSHGTERTIKVSSGPKVCNQRPAVDPLFES
                     VAEYAGRNCIA"
     gene            complement(863..1669)
                     /locus_tag="PKH81_04750"
     CDS             complement(863..1669)
                     /locus_tag="PKH81_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNQ97379.1"
                     /translation="MSNVPGDNPDNSLPIGLLQRFREYAYERYGIHFHDAKIDILKMK
                     LQKMSSLHGFSLPGFYSLLATGDPDAGELFLGEIAVGHTFFFRESDHFTHLVTDITNR
                     SLQHPVVWCAASSSGEEPYSIVISLLEAGIHSFKVLASDVKPEALAAIHRGRYHVNQL
                     QNVDASLQKRYFTRLDEYTFAVRPELRKYLAIKRLNLHESFFFETPFDYVFCRNVMIY
                     FDERGRNRVLHTLVDNLAPKGVLYVGHSEALLTTTPNIRKEGPASYRRVG"
     gene            complement(1675..>2356)
                     /locus_tag="PKH81_04755"
     CDS             complement(1675..>2356)
                     /locus_tag="PKH81_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97380.1"
                     /translation="GKAADDIKTVIDVIDDIAFQTNMLALNAAVEAARAGEAGMGFAV
                     VADEVKNLANRSASSAKETAQMIKTTLQKTEEGQVLTKELSEIFKEIMLNSSKSNEMT
                     KEVETASRQQDEGISQVNKAVVQLDTVVQQNAAASEETASSAEELQSQVVSLNEIIEN
                     LSVLVLGGKGVSSASQKPEQQKLIAVKRGVETGKSLKHKLDGESITPRGKRIVFDDDP
                     EFQGEQEL"
BASE COUNT          644 a          564 c          474 g          674 t
ORIGIN      
        1 gctatgcagt tacgtcccgc gtactccgcg acggactcga acagtggatc tactgcaggt
       61 cgctggttac atacctttgg acccgatgat acttttatag ttcgctccgt tccatgcgat
      121 ctaagaagca tatgatagtt tcctggagcg atataaatag ttccagagag tagtcgctcg
      181 ccgttttccg cttccttaac ccgtgccgaa cttagatcat taagacgaga ggcaaaagtg
      241 gcggtaaatc gttcgggcat atggataaca actaatgttg gggggaaact atcctcccac
      301 tcccgaaata aggcttcaag tgctattgtc cccccagtag atgccccaat agcaattaac
      361 tgattagttg ttttcaagtc tgcaagatat cgtctccggt tcgtttgctt tattggcgtt
      421 agtaaagatg atggagaagg aagactttgc tttaatcgag caatattagc cttacttgct
      481 gctacgatac gttccgccaa atagggaacg acttcctcaa ccgaaaaggg gcctccgggc
      541 ttgggaacaa cttccactgc tcccagttca agagccttca tagccgccgc attcgcgtct
      601 ttagcaagtg aagaaactac tataactggt tttggaaatt gttccattag ttgacgcaaa
      661 aaagtaagcc cgtccatcct gggcatttca atatcaaggg ttagtacatc tacgtcaatt
      721 ttctcaagct tgtcacgtgc aatatacgga tccatagctg tgccgacaat ttctattcgt
      781 gggtcacgag aaagccgttg ggcaagaatc tcccggacga tcgcggaatc gtcaactatc
      841 aacaccttta ttttttccat gtttatccaa ccctacgata agaggctggt ccttctttcc
      901 ttatattggg cgttgttgtt aacaatgcct ctgagtgacc aacatagagc actcccttgg
      961 gcgctagatt atctaccagg gtatgcaata ctcgattcct tcccctctcg tcaaaataaa
     1021 tcatcacatt tctgcaaaaa acataatcaa aaggagtctc aaaaaagaag gactcatgaa
     1081 ggtttaaacg cttaatcgca agatattttc gtaactctgg gcgaactgcg aaggtatact
     1141 catcaagtcg agtaaaatat cgcttctgca atgaagcatc aacattttgt aattgattta
     1201 catgatatct tccacgatga attgcagcaa gggcttccgg ttttacatcc gaagctaaaa
     1261 ccttaaaact gtgaatacct gcttcaagaa gagaaataac aatagagtat ggctcctctc
     1321 ctgatgaact tgcggcgcac cataccacag gatgttgtaa agatcgattg gttatatctg
     1381 ttactaaatg agtaaaatga tcgctttctc taaagaaaaa agtatgcccg accgcaatct
     1441 ctccaagaaa aagctctcct gcgtccgggt ctccggtggc aagcaaagaa tagaaacccg
     1501 gaagagaaaa gccatgcaaa gacgacatct tttgtagctt catctttaag atatcaatct
     1561 ttgcatcatg gaaatgaata ccataacgtt cataggcata ctcgcgaaat cgttgaagca
     1621 aaccaatcgg tagtgagtta tcgggattat cgccaggaac attactcatg gaattcacaa
     1681 ttcttgttct ccttgaaatt ccggatcatc gtcaaacact atccgctttc cccgtggagt
     1741 aatactctct ccatccagct tgtgcttaag cgattttccg gtctccacac ctcttttaac
     1801 ggcaatcaat ttttgttgct caggtttttg cgaagcggaa gaaacaccct ttccacctaa
     1861 tacaagaaca gaaagattct cgattatttc attaagagaa actacctggc tttgtaattc
     1921 ctcggcagaa ctcgccgttt cttccgatgc agctgcgttt tgttgaacaa ccgtatcaag
     1981 ctgcactacg gccttattga cctggctaat accctcatct tgttgccgac ttgcagtttc
     2041 gacttctttg gtcatttcgt ttgattttga ggaattaagc atgatttcct tgaaaatctc
     2101 agaaagctcc ttggtaagga cctgcccttc ttctgttttc tgcaaggttg tcttaatcat
     2161 ctgggcagtt tcttttgcgc ttgatgcgct tcgatttgcc aaattcttaa cctcatcggc
     2221 gacaacagca aaccccattc ccgcctcacc tgctcgcgcc gcttcaacag ccgcgttaag
     2281 ggcaagcata ttggtttgga acgcgatatc gtcgataaca tcgataacag ttttaatatc
     2341 atcagcggcc tttcca
//
LOCUS       DAOQXQ010000239         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_50999_length_2353_cov_1.960400, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000239 DAOQXQ010000000
VERSION     DAOQXQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50999_length_2353_cov_1.960400"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKH81_04760"
     CDS             <1..825
                     /locus_tag="PKH81_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HNQ97381.1"
                     /translation="PLIEEIQAVGETPLAEEAPQEIDPVRQNVLTQTTGTTKSINDPS
                     SRSSIYSSSNPLEIFFFGDSQVFSLGSGLTRLVGKGGPVKVDYLAVHSSGFIRSDYFD
                     WPSKLLDTFKDRHYASCAVMMGMNDYQSFRDETGTILKKHTPSWENAYRERCRSIIDL
                     ALSEVVHVYWIGMPRVKNRVYDESLRYIDSVQASVAEEYSPNLVTRISLSDSIPGSGK
                     EYSQTIVLDGGQTIRVMSDDGSHFTVEGGQLAMRGLFDNLVQDFSFAELPVANLPQ"
     gene            863..2095
                     /locus_tag="PKH81_04765"
     CDS             863..2095
                     /locus_tag="PKH81_04765"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNQ97382.1"
                     /translation="MLKRNPCIAHLSSGYLFPEISRRRAAFAKAHPEAKIISLGIGNT
                     TEPLTPHVVSGLHEEVTRLGTAEGYSGYGDETGSTALRDKISKVLYKGMVSADEVYIS
                     DGAKCDIGRLQYLFGRDVTVAVQDPAYPVYVDGSVMIGAAGPVTEGGSGYSGIVYMPC
                     TADNNFFPDLAAIPKNALIYFCSPNNPTGAVATREQLEALVARAIEQESIIIFDSAYS
                     SYIRDPQLPRSIYEIPGSRNCAIEVSSFSKPIGFTGVRLGWTCIPSELTFADGTAVGK
                     DWSRLVTTIFNGASNIAQSGGLASLDEKGLAETRSLTDYYLENARLIREALCAPSFAK
                     AGLTLYGGDNAPYVWARFPGKKSWQIFDLLLERSHVVCTPGAGFGPAGESFIRFSSFG
                     HRAQIQEACERLAKLDID"
     gene            complement(2124..>2353)
                     /locus_tag="PKH81_04770"
     CDS             complement(2124..>2353)
                     /locus_tag="PKH81_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97383.1"
                     /translation="EYYEGTDSPENREFASLVLGGGLTWVPKADMSFFILGSGSLYTF
                     SLEEDEDDLDRFLFEIKIGTRIMTGNQAGHR"
BASE COUNT          583 a          531 c          567 g          672 t
ORIGIN      
        1 ccgcttatag aggaaatcca agctgttggc gaaactcccc ttgcagaaga agccccgcaa
       61 gagatagatc ccgtccgcca aaacgtatta acccaaacca ctggaactac caaatccata
      121 aatgatccct cttcccgctc ttcaatatat tcaagctcca atcctttaga gatttttttc
      181 tttggagatt ctcaagtgtt cagtctcggt tctggcttaa ccagacttgt tggcaaaggt
      241 gggcctgtaa aggttgacta tctggcagtg cattcttccg gatttattcg cagtgattac
      301 tttgactggc cttcaaaact tttggatacc tttaaggatc gccattatgc atcgtgcgca
      361 gttatgatgg gtatgaacga ttaccaaagt tttagggacg agacgggaac tatattaaaa
      421 aagcacactc cttcctggga aaatgcgtat cgagagcgct gtagatcgat catcgatttg
      481 gcacttagcg aggttgttca tgtgtactgg attggaatgc ccagggttaa aaaccgggtg
      541 tatgacgaaa gtctgcggta tatcgattca gtccaggcct cggttgccga ggaatacagt
      601 ccaaatctgg taacccgaat ttcactgtcc gattcaatac cgggtagtgg caaagaatac
      661 agtcagacca ttgttctcga tggagggcaa acgattcgtg ttatgagcga cgatggatct
      721 cactttaccg tagaaggcgg tcaacttgcc atgcgtgggc tttttgacaa tcttgtacaa
      781 gacttttcct ttgccgagct tccagttgcc aatcttccgc aataattgat atacttccct
      841 tgaatacaaa gagggagtag acatgctcaa acgtaatccg tgcattgcac atcttagctc
      901 aggttatttg tttccggaaa tatctcgacg cagggccgcc tttgccaagg ctcatccaga
      961 ggcaaagata atcagcttgg gtattggtaa taccaccgaa ccgcttacgc ctcatgttgt
     1021 ttcgggtcta catgaagagg ttacccgtct tggaacagcc gaaggatatt ccggctatgg
     1081 agacgagacc ggatcaaccg ccctgcgaga caagatttct aaagttctct ataagggtat
     1141 ggtcagtgct gatgaggtat atatctcgga cggtgccaag tgcgatattg gtcgtttgca
     1201 gtatcttttt ggccgtgatg taactgttgc ggtacaagat cctgcgtatc cggtgtatgt
     1261 agacggctct gtcatgattg gcgctgccgg tcctgttacc gaaggtggaa gcggttattc
     1321 cggtattgtg tatatgccct gtactgcgga taataatttc tttcctgatc ttgccgccat
     1381 tccaaaaaat gctttgatat atttctgttc gcccaataat cctaccggtg cagtcgcgac
     1441 acgggagcaa cttgaagctt tggtagctcg agctatagaa caagagtcga tcatcatttt
     1501 tgattctgca tattcatcgt atatccgcga tccgcaattg cctcgatcaa tctatgaaat
     1561 tcctggctca agaaactgtg ccatcgaggt aagctctttt tcaaaaccca tcggttttac
     1621 tggtgtgcgt cttggttgga cttgtattcc ttcggaatta accttcgccg atggaactgc
     1681 ggtgggtaag gattggagtc gtcttgttac cacaatcttt aacggagctt caaatattgc
     1741 acaatctggc ggcttggctt ctcttgatga aaagggtttg gcagaaactc gctcccttac
     1801 cgattattat ttggaaaatg cccgtttgat tcgggaagcc ctctgtgccc cttcttttgc
     1861 aaaagctggt ttgaccttgt acggtgggga taatgcaccg tatgtgtggg cgcggtttcc
     1921 cggaaaaaag agctggcaga tatttgactt acttcttgag cgctctcatg tagtgtgtac
     1981 tccaggggca ggttttggtc cagccggaga atcttttatt cgatttagtt catttggaca
     2041 tcgtgcgcag attcaagaag catgcgaacg tcttgcgaag cttgatatag actaagaaga
     2101 tggcgtgatg gactggccgg ttattatcta tgaccggcct gattccccgt catgatgcga
     2161 gtgccaattt taatttcgaa gaggaatcga tcaagatcgt cttcgtcctc ttcaagcgaa
     2221 aaggtataca gggaaccaga gccaagaatg aaaaaactca tatcggcctt gggtacccag
     2281 gtaaggcctc ctcccagtac aagagacgca aattccctgt tttcagggct atcagtcccc
     2341 tcgtagtact cca
//
LOCUS       DAOQXQ010000240         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51268_length_2345_cov_2.411354, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000240 DAOQXQ010000000
VERSION     DAOQXQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51268_length_2345_cov_2.411354"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKH81_04775"
     CDS             <1..1020
                     /locus_tag="PKH81_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNQ97384.1"
                     /translation="ELSRRLARKSTCLAIPAFKLAPLASAMLSRIARSPSHAPQWVIL
                     ATAFASFCFSLLFHGSMTEALIAFVIGALMRFALFALSPLVLSSFIQAMVGGAIISLL
                     ASCGVALGLIQSSGNVSIAVLMSLVPGVAIVNAIRDIIAGDLVAGSARVLEAFVIAAG
                     LSIGAALGLFLFPVPVGYASAILARPERLSAFALAFGATASFAYFFHITRYDLLWASL
                     SGALGWLVYLWAAESSLSPISGFFAGALFVGLAAEFFAFFFKKPATVYIVPGIIPFVP
                     GGGMYETMLFAVQGDIAAASVTGFNTLSAAASIAVGIALASSLSRLLSRLGKLVSPLH
                     HLRHF"
     gene            complement(997..1806)
                     /locus_tag="PKH81_04780"
     CDS             complement(997..1806)
                     /locus_tag="PKH81_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNQ97385.1"
                     /translation="MHFLGVFYASLYDILLFMCKKHFSLRSLLFRRDSTIIDHSPEGF
                     YLPAHRRIGTSMIHRPDIVWIDSGNTKEEILEIIRNNPGLHWFPVCANTVDSVLGILS
                     DRDFLESLTHPSWPGLRSLVQRPLYLPETITIARAFSLLEENNCPLALVIDEWGGIEG
                     MITSASLVSDLLKEFQGEETEEDPDLFKREDGSYLVAGQVRMDDIRELFDIEDTGEKN
                     YYTLAGFLLSLNGSIPKTGDRIICGSYQFEIVDMDGHRIDKVLISKMTEMV"
     gene            1880..>2345
                     /locus_tag="PKH81_04785"
     CDS             1880..>2345
                     /locus_tag="PKH81_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNQ97386.1"
                     /translation="MLVMKFGGTSMGSAERMNSSLAIIAQRAQDNRVCVVVSAVGGVS
                     NRLQAAIDEAMRTKDEQSADVASLVEPFVSIHNTIIDELSRFAPGFRPDVVHQAIEPL
                     FDEYSRLLTAVNAFGECPQSIECRIMGLGERACIPIMAELSHTRFPAVTVLDS"
BASE COUNT          471 a          597 c          581 g          696 t
ORIGIN      
        1 gaactctctc gccgtctcgc ccgaaaaagt acctgcttgg ctatacctgc tttcaagctt
       61 gccccccttg cctcggcgat gctctctcgt atcgcacgct ctccatctca tgcacctcag
      121 tgggtcattc tggctacggc ctttgcaagt ttttgctttt ctcttttatt tcatggctct
      181 atgaccgaag cccttatcgc ctttgtcatt ggcgccctta tgcgcttcgc tctttttgcc
      241 ctgtctcccc tggtcctttc ttcgtttatt caggctatgg ttggaggggc tattatttct
      301 ttgttggcct cttgtggggt cgcccttggc cttattcaat cgtcggggaa tgtcagtatt
      361 gccgttctta tgtctcttgt tcccggcgta gcgattgtga acgccatccg tgatattatc
      421 gcaggggatt tggtcgccgg gtcagccagg gttcttgaag cctttgtcat agccgcaggg
      481 ctgtccatcg gcgcagccct tggtcttttt ctttttccgg ttcctgttgg ctatgcgagc
      541 gcgattcttg cccggccaga gcgtctgagc gcctttgcct tggcctttgg agcgaccgct
      601 tcctttgctt atttttttca catcacccgc tatgacctat tgtgggcgtc actttccgga
      661 gcccttggct ggctcgtcta tctgtgggct gcagagagca gcctcagtcc catttcgggc
      721 ttttttgccg gggctctttt tgttggcctt gccgccgaat tttttgcatt cttttttaaa
      781 aaacctgcca ccgtctatat agtcccggga attattccct ttgttcccgg tggaggtatg
      841 tacgaaacga tgctttttgc cgttcaagga gatattgccg cagcctcggt aaccggtttt
      901 aataccctca gcgccgcggc ttctattgcg gtgggcatcg cccttgcttc ttccctttct
      961 cggctcttat cccgactggg taagctggtt agccccttac accatctccg tcatttttga
     1021 aatcaatacc ttgtcgatgc ggtgtccgtc catgtcaaca atttcgaact ggtatgaacc
     1081 acagatgatg cgatccccgg ttttgggtat gctgccatta agggataaga gaaatcctgc
     1141 cagggtgtaa tagttttttt cgccggtatc ttctatatca aagagctccc gtatgtcatc
     1201 catcctgacc tgtccggcga ccaggtagct tccatcttcc cttttaaaaa gatcggggtc
     1261 ttcttccgtt tcttcacctt gaaattcctt gagtaaatcg gataccagcg aggcgctcgt
     1321 aatcataccc tctatccctc cccattcgtc gatgaccaag gcaaggggac agttgttttc
     1381 ttcgagcagg gaaaaagctc gggcaatggt tatagtttcc ggaaggtaca ggggcctttg
     1441 tactaacgag cgaagtcctg gccaggaagg atgagtcagt gattctaaaa agtccctgtc
     1501 cgacaggatg cccaaaacgg agtctacggt gttggcgcag acggggaacc agtgcaggcc
     1561 tgggttgttc ctgattattt ccagaatttc ctctttggtg tttccactgt caatccacac
     1621 aatgtccggg cggtggatca tggaggtacc gattcgccgg tgtgcgggta aataaaagcc
     1681 ctcgggcgaa tgatcgatta tagttgagtc ccggcgaaac aagagagagc gaagtgaaaa
     1741 gtgtttcttg cacataaaca ggagtatatc atataaagat gcataaaaaa cacctaaaaa
     1801 gtgcataaat atgcattttc acccctagaa agaatctgaa gaatcaagta tggtgaggct
     1861 aacgcataaa gaggtgtgta tgcttgttat gaaatttggt ggcacgtcca tgggatctgc
     1921 cgaacgcatg aacagttctc ttgctattat tgcccaacgc gcccaggata atcgggtgtg
     1981 tgtggttgtt tctgctgtag gcggggtgtc caaccgtttg caggctgcta ttgatgaggc
     2041 tatgcgcaca aaagacgaac aatcggcgga tgtggcatcc ctggtagagc cctttgtttc
     2101 tatacacaac acaataattg acgagctttc ccggtttgca ccgggattta ggccggatgt
     2161 ggtacatcag gcaatcgagc cgctttttga tgaatacagc cgcctgttaa ccgcagtcaa
     2221 cgcttttgga gaatgtccac aatccatcga atgccgaatt atgggcttgg gtgagcgtgc
     2281 gtgcattccc ataatggcag aactctcaca cacccgtttt cccgctgtta ccgtgttaga
     2341 tagtc
//
LOCUS       DAOQXQ010000241         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51354_length_2342_cov_2.722781, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000241 DAOQXQ010000000
VERSION     DAOQXQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51354_length_2342_cov_2.722781"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKH81_04790"
     CDS             <1..885
                     /locus_tag="PKH81_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97387.1"
                     /translation="HRTILSRSYGELAVSLRLPTQIKQPEDVFIKARLYVNDGSDFPE
                     VPVSPLSFTLAEDHNDSRVYRFESSPVYLAPGQYRIKVMVEGRVYWKSFTLYPLKGSN
                     GEDGDTLSLSFSLAGIESLPLSLTLGSYDAVSGRNITSLSRYLFLVGSSWVPLSDLPP
                     SQLTSSSIVKCRVEASGYYSEEFSLKVAPHQSELDIQANLVPLPGTLTVNAPKKTMRL
                     TINGKRSLILGGRELQEASLAGFSGGKQSWEIPSGRYDIEVGVAKSLSRVSVEVTPGE
                     ETRLVISESHGVYQIQKE"
     gene            889..1791
                     /locus_tag="PKH81_04795"
     CDS             889..1791
                     /locus_tag="PKH81_04795"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNQ97388.1"
                     /translation="MSLLLKRLVITGLGLFGALMVWPFLLFIQSAQSAFPGYLSFSVV
                     QGAFLGLVFGALFGSIEGIVVSSRTKAFKGILFGMILGIGFGVLGVVLGQTFLFYAAG
                     LIVEVEGVTRLILVAFANGASWVIIGMCLSMIEGLRSLSLRRILVGLWGGMIGGLIGG
                     FALQLVLAYKPGNGIALLVGLSLFGLSLTFFYSLFEQGFSLGSIKLLNGPLRNKEYSI
                     TRNRMSVGTKDSCDIVLTGYRDIKDVHAFLITKKGRVSLVAAQEGNPVKVNDENRSEC
                     SLRREDVFAIGNAKFMYGFFSMGV"
     gene            1794..>2342
                     /locus_tag="PKH81_04800"
     CDS             1794..>2342
                     /locus_tag="PKH81_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ97389.1"
                     /translation="MKCLYNAKTVCSIAGAFLLFCNTLFFAQEKSLSITQIDNSRLVS
                     TQTIRLYLSIHGESFSPDTQNIIVEESDNGKDFKKVRLVEVTKNANQSEGISFFLLLD
                     NSGSMWDDLEGKATSDPAMTRMNHAKRAIAAFSQSLGVHDRLALATFNTQYTIVQPMT
                     SDAASIPLALEGVTRPAREEGYT"
BASE COUNT          552 a          503 c          572 g          715 t
ORIGIN      
        1 catcgaacta tactatctcg ctcatatgga gagcttgcgg taagccttcg acttcccacg
       61 cagataaaac agccagagga tgtttttatt aaagccaggc tctatgtgaa tgacggctcc
      121 gattttccgg aagtcccggt ctctccactt tcttttacgc tcgcagaaga tcacaatgat
      181 tcgcgcgtat accgctttga atcatcgcct gtgtatcttg ctccgggtca atatcgtatt
      241 aaagtgatgg tagaggggcg agtatactgg aagtcattta ctctctatcc tcttaagggc
      301 agtaatggtg aagatggaga tacactgtct ctgtcgttta gtcttgccgg cattgagtcg
      361 cttccccttt cgctaacgct tggttcatac gatgctgtaa gcggacgaaa cattacctcg
      421 ctttctcgct acttatttct ggttggctct tcatgggttc ctctatctga tctgcctcct
      481 tctcaactaa cttcttcgtc catagtaaaa tgcagagtag aggcgagcgg ttattattcc
      541 gaggaatttt cccttaaggt tgcgccgcat caaagcgaac ttgatattca ggcgaatctg
      601 gttccgcttc ctggcacgct tacagtcaat gcccctaaga aaaccatgcg ccttaccatc
      661 aatggaaagc gatctctcat tcttggcggc agggaattac aagaggcctc gttagcgggt
      721 ttttccgggg gaaaacaatc ttgggaaatt ccttcgggaa gatatgacat tgaagtggga
      781 gttgctaaaa gtctttcccg ggttagcgtt gaagttaccc ccggagagga aactcgactt
      841 gttataagcg agtcacatgg tgtgtaccag atacaaaagg agtaattgat gtctcttctg
      901 ttaaaacgat tagttattac cggcttgggt ctttttgggg cccttatggt atggcccttt
      961 ttgctgttta ttcagtccgc tcaatcggcc ttcccggggt acctcagttt ttctgttgta
     1021 cagggcgctt ttcttgggct tgtctttggc gccctgtttg gctccataga aggaattgtg
     1081 gtttcttccc ggacaaaggc ttttaaggga atactctttg gcatgatctt gggtattggg
     1141 tttggtgtgt tgggtgttgt cctgggtcaa acctttttgt tttacgccgc aggacttatt
     1201 gtagaagtag aaggagttac ccggcttata ctggttgcct ttgcaaatgg ggcgagttgg
     1261 gtaatcatag gaatgtgtct ttccatgatc gaagggctca ggtctttatc tctgaggcga
     1321 atactcgtag gcttgtgggg agggatgatt ggcggtctta ttggcgggtt tgccttgcag
     1381 ttagttctag cctataagcc gggtaatggt attgcgctgc ttgtcggtct gtccttattt
     1441 ggactttcgc tcaccttttt ttactccctg ttcgagcagg gtttctctct tggttctatc
     1501 aagctgttaa atgggccatt acggaataaa gaatacagca ttacccgcaa tcgcatgtca
     1561 gttggaacaa aggatagctg cgatattgta ttgaccggat atcgggatat aaaagatgtt
     1621 catgcattct tgataactaa aaaaggacgg gtttcgcttg tggcggcgca agaaggtaat
     1681 cctgttaagg taaatgacga aaatcgcagt gaatgttcac tcaggagaga agatgtgttc
     1741 gccataggaa acgcgaaatt catgtatggc ttttttagta tgggagtgta agaatgaagt
     1801 gcttatataa cgctaaaact gtttgtagta tcgctggagc ctttctcctt ttttgtaaca
     1861 cgcttttttt tgcccaggag aagtctcttt caatcacaca gatagacaac tcacgacttg
     1921 tgtcaactca aaccattcgg ctctatctta gtatccacgg tgaatctttt tccccggaca
     1981 cacagaacat cattgttgag gaatccgata atggaaaaga tttcaagaag gttcgtcttg
     2041 tagaggtaac caaaaacgca aatcagagcg agggaatctc gttcttttta ctcttggata
     2101 attccggaag catgtgggat gatcttgaag ggaaggccac atccgatcct gctatgaccc
     2161 gaatgaatca tgcaaaacgc gcgattgctg cttttagcca aagtcttggc gttcacgatc
     2221 gccttgccct tgctacgttt aacactcaat atacaattgt tcaaccaatg acttccgatg
     2281 cggcatccat tccattggcc ctggaaggtg ttactcgccc tgcgcgagag gaaggctata
     2341 cc
//
LOCUS       DAOQXQ010000242         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51456_length_2339_cov_2.327058, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000242 DAOQXQ010000000
VERSION     DAOQXQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51456_length_2339_cov_2.327058"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKH81_04805"
     CDS             complement(<1..621)
                     /locus_tag="PKH81_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ97390.1"
                     /translation="METHVPVDEDICIKRAREVFGIPYLFPWQRLVIANILDAIASKN
                     APMDEEEQFDEDGVLRGQQIVLLPTGAGKSLCFQLPAVLLEGQTLVIYPLLALMGDQE
                     RRLREAGVETVIFRGEQDANTRQDCLDRLEGKNGRSAAQLIIANPEVLAGEKLLDRIA
                     RRNIAHIAIDEAHCVSEWGDSFRPAYLGLGDIIKRINAPAVTAFTAT"
     gene            704..1693
                     /locus_tag="PKH81_04810"
     CDS             704..1693
                     /locus_tag="PKH81_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ97391.1"
                     /translation="MKKQTIRSIIHAIIPLTLLVAFAFALYFPEVVNPFMRLQYKSSA
                     GLGTQTITLPATEEEGSKKKSKGDVSVVFKLVPAGTAPRGCTDVSDGTVVTEEFVLGE
                     TEVTRGLWRAVTSAPSLASYRFSGLVLCDGDAKLPAVKVSWREAIVFCNALSELMGNQ
                     PVYYQDSAFLIPVRDPGFLETRNDALFVRPGSDGFRLPTSMEWELAARYRDGVEWTPG
                     SNPSGSSVPYYEYARSDIYAVYNAPSIAPVRSLVSNQLGIFDMSGNAWEWCYDLFVKD
                     STAVVDDETRIKRIVRGGSWMGNAYRLQVGGEFGSLPGITEIGQGFRLARSRW"
     gene            complement(1698..>2339)
                     /locus_tag="PKH81_04815"
     CDS             complement(1698..>2339)
                     /locus_tag="PKH81_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNQ97392.1"
                     /translation="EGGHSMHSHYSARSNPFMCYEYTIFEAEVASTFNEDLLFRSLIK
                     KADEPALKAYLLSTRVSDILATLFRQTMFAEFEKKTHALVESGTPLTADKLRSEYRSL
                     LEAYFGSEMQFEQESDLECLRIPHFYNAFYVYKYATGISAAMALANRVYNGGESERKD
                     YFKFLASGGSRYPIDALKVAGVNMEEKEPVRAACETFVATLDELDTALKAINR"
BASE COUNT          522 a          589 c          590 g          638 t
ORIGIN      
        1 cgttgcggta aaggcagtta ccgcaggcgc gttaattcgc tttatgatgt cgcccaagcc
       61 aagataggcc ggtcggaagc tgtctcccca ttctgaaacg caatgagcct cgtcaatggc
      121 aatgtgtgca atatttcgcc gggcaattcg atcaaggagt ttttctcccg caaggacctc
      181 cgggttcgcg atgatcaatt gtgccgcact ccgtccattc tttccttcca accggtcaag
      241 gcagtcttgc cgcgtgttcg catcctgctc tccgcgaaag ataaccgttt ctacgcctgc
      301 ttccctgagc cttctctcct gatcgcccat cagggcgagc aggggataga taaccagggt
      361 ttgtccttcc agaagtaccg caggtaattg aaagcacagg gatttaccgg cacccgttgg
      421 cagaagcact atctgttggc cccgcagtac cccgtcctca tcaaactgtt cttcctcatc
      481 catcggggcg tttttagaag ctattgcgtc aaggatattg gctatgacca atcgctgcca
      541 gggaaataga tagggtatgc caaagacttc ccgcgcacgc tttatacaga tatcctcatc
      601 aacaggtacg tgtgtttcca tgccttgtat tgtgtaaaga aaaggtgatg ctggcttcaa
      661 aagatagttt tctttattgt ggggccggta tatagtaagt gtcatgaaga aacaaactat
      721 tcgatcaatt attcatgcaa ttattccgct cacgctttta gttgcctttg cctttgccct
      781 gtatttcccg gaagtagtca acccttttat gcgacttcaa tacaaatcct ctgcgggact
      841 gggcacccaa actattaccc ttcctgccac tgaagaggag ggctcaaaga agaagtccaa
      901 aggggacgtg agtgttgtct ttaaacttgt cccggccgga accgctcctc gtggttgtac
      961 cgatgtgagc gatggcaccg ttgttaccga ggaatttgtc cttggagaga cagaggttac
     1021 ccggggcctg tggcgtgcgg tcacttcggc tccttccctt gcttcctacc gtttttccgg
     1081 tctcgtcctt tgtgatgggg atgcaaaatt accggccgtt aaggtaagct ggcgggaagc
     1141 gattgtgttt tgtaatgcct tatccgagct tatgggaaac cagccagtct attatcagga
     1201 ttcagccttt ttgattcccg ttcgcgatcc cggttttttg gaaacccgta atgatgccct
     1261 cttcgttcgg ccgggttccg acggttttag gctacctaca tctatggaat gggaacttgc
     1321 ggcccgctac cgcgatggtg ttgaatggac gcccggttca aatcccagcg gtagttccgt
     1381 accctattac gagtatgctc gctccgatat atacgcggtg tataatgcac cgtccatagc
     1441 cccggttcgc tctttggttt caaatcagct gggaatattc gatatgagcg gaaatgcctg
     1501 ggagtggtgt tatgacctgt ttgttaagga tagtacggct gtagtggatg atgaaacgcg
     1561 aatcaaacgc atcgttcgcg ggggaagctg gatgggaaac gcctatcgct tgcaggtagg
     1621 cggggaattc ggcagcttgc ccggaataac cgaaattggg caaggctttc gccttgcccg
     1681 aagtcgctgg taatccctta ccggtttatc gcttttagtg ctgtatccag ttcgtccagg
     1741 gtggcaacaa aggtttcgca tgccgcccgt acgggctctt tttcttccat attcaccccc
     1801 gcaaccttta gcgcgtcgat tgggtagcgg gaacctcccg aagcaagaaa tttaaagtag
     1861 tcttttcgct cgctctcccc gccgttatat accctgttgg caagggccat cgcggccgag
     1921 atgccagtgg cgtatttata cacataaaag gcgttgtaaa agtgcggaat acgcaagcac
     1981 tccaaatcgc tttcttgctc gaattgcatc tcgctgccaa agtatgcttc caacagcgaa
     2041 cggtattcac ttcttaattt atcagcagta agcggtgtcc ccgattcaac cagggcgtgt
     2101 gtttttttct caaattcagc gaacatggtt tgtctgaaca gggtagccaa tatatcgctt
     2161 accctggtgg acaatagata agcttttaat gccggctcat ctgccttttt gataagcgac
     2221 cgaaaaagaa ggtcttcgtt aaaggtcgag gcaacttccg cctcgaatat ggtatattca
     2281 taacacataa agggatttga ccgggctgaa tagtgtgaat gcatggagtg gccgccctc
//
LOCUS       DAOQXQ010000243         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51554_length_2336_cov_2.770715, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000243 DAOQXQ010000000
VERSION     DAOQXQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51554_length_2336_cov_2.770715"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKH81_04820"
     CDS             complement(<1..403)
                     /locus_tag="PKH81_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97393.1"
                     /translation="MLSGTLEQMMKFFYRRIALITALYLTIIFGIFALQFTRGNTFSF
                     TIGALSVSGSTETDEKGVEKPALPLHLGAWGLDFFLDEHHSLMAYTSGNNGVPVQVES
                     WSRQDDGFSLVFARGIRLVFSTEKRGDIDVLS"
     gene            435..1883
                     /locus_tag="PKH81_04825"
     CDS             435..1883
                     /locus_tag="PKH81_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97394.1"
                     /translation="MSVTVKHAPIAALLIASMLLFSCATEKAAVKTDAPPVGKTEPNK
                     GEWSRIAFAEDLARLSNQGRWDEVFALFDSVPQKEAESADIGRLKLSMLISARQLDAA
                     TDTANDLEKRFPKDADILYTRAVLAGAQNNAGARKSYLDKTLAVDKNHSDAMAALGLD
                     LVSLKNYKLAKTWLVKAVAANPNNLDALFGLGRTYYMENELDKARETMDLALERDSSW
                     SILWAERARIRAEQNDVKGAIEDVKKACEIDGNIYSHWVDLGNYYITGFKKAEARDAF
                     SRAIEIDGSQYLAYIYRAGLNDDLGNLDEAIADYRKVCEIFPNYHFAFESLGILLWGK
                     GDWQGSREAFRTALSYRPKNSSYALMTTLCFYREGKEKEAKEFMTKYLALLDRTTTEY
                     FLCRLFVDLVGDADVLNRIMKEKNVNVRNRMLFYSAMYYQLFQSKSIAQKYFLEVISV
                     PAPSFFEYRLSRWAITELEGTVREDTGRVSGT"
     gene            1894..>2336
                     /locus_tag="PKH81_04830"
     CDS             1894..>2336
                     /locus_tag="PKH81_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ97395.1"
                     /translation="MNKPLRTLLRTLDRSRAVLVQAHDFPDHDAIASAHALAALLSRK
                     GFNASACASFQGNSISASIMIDRLAITLHAAEHLTVDEGTQTILVDGSCSGGTVSRVV
                     GTLTGIIDHHPGNIPQDCPFTDIRVEAGSCSAIIWSYWHEAGENP"
BASE COUNT          614 a          628 c          579 g          515 t
ORIGIN      
        1 gggacaggac atcgatatca cccctttttt ccgtggagaa tacaagacga atgccacggg
       61 caaagacaag agaaaagccg tcatcctggc gggaccatga ttcaacttgt acaggaacgc
      121 cattatttcc gcttgtatat gccatcaacg aatggtgttc gtccaggaaa aaatcgagcc
      181 cccatgcacc aagatgcagc ggaagcgcgg gcttttccac ccctttttcg tcggtttctg
      241 ttgacccgga gacagaaagt gcaccgattg taaaagaaaa ggtgtttccc cgggtaaatt
      301 gaagggcaaa tatgccgaaa atgatcgtga ggtataacgc agtaatcaac gcaattctgc
      361 gataaaagaa tttcatcatt tgttcgagtg tacctgaaag catggaacaa aatcaacgac
      421 tttaagggga cgcaatgtca gtaactgtga aacacgcgcc aatcgcggcg cttttaatcg
      481 cttctatgct tcttttctcc tgcgcaacgg agaaagcggc cgtcaagacc gacgcgccac
      541 ctgtgggaaa aaccgaaccc aacaagggtg aatggtcgcg catcgccttt gcagaggatc
      601 ttgcccgcct gtcaaaccag ggccgatggg acgaagtgtt tgccctgttt gactcagtgc
      661 cacaaaaaga agcggaatcc gcagatattg gcaggctcaa gctttccatg cttatttccg
      721 ctcgacaact ggatgcagca accgacacag ccaacgactt ggaaaaacgc ttccccaaag
      781 atgcggatat cctgtatacc cgagccgttc ttgcaggagc ccaaaacaat gccggcgcgc
      841 gaaaaagcta cctggataaa acccttgcag tagataaaaa ccacagcgac gccatggccg
      901 cccttgggct ggatcttgtg tccctaaaga attacaagct cgctaaaaca tggctcgtta
      961 aggcagtggc cgccaacccc aacaaccttg acgccctgtt tggactgggc cgaacctatt
     1021 acatggaaaa cgagctggac aaggcccgcg agaccatgga tttggctctt gaacgcgact
     1081 catcatggag catcctttgg gcagagagag ccagaattcg cgccgagcaa aacgacgtaa
     1141 aaggcgccat tgaggatgtt aaaaaagcat gcgagataga tggcaatatc tacagccact
     1201 gggtggacct tggtaattac tatataaccg gctttaaaaa agccgaggcg agggacgcat
     1261 tctctcgcgc aatcgagata gatggaagcc aataccttgc gtatatctat cgcgccggac
     1321 ttaatgatga ccttggcaat cttgacgaag cgattgccga ctaccgcaag gtttgcgaga
     1381 tttttcccaa ttaccatttt gcctttgaaa gtcttggcat tcttttgtgg ggaaagggcg
     1441 actggcaggg tagccgcgag gccttccgca ctgccctttc ataccgacca aaaaacagct
     1501 cctacgccct tatgaccacc ctttgctttt accgcgaagg caaggaaaaa gaggccaagg
     1561 agtttatgac caaatatctg gccctgttgg accgaacgac gaccgaatac tttttgtgcc
     1621 gcttgtttgt ggatctggtt ggggacgccg acgtacttaa tcgtataatg aaagaaaaga
     1681 acgtaaacgt cagaaaccgg atgctctttt attccgcgat gtactaccaa ctgttccaaa
     1741 gtaaatccat cgcccaaaag tacttcctgg aggttatctc cgttcccgcg ccaagcttct
     1801 ttgaataccg cctttcccgg tgggctataa ccgaacttga gggaacggta cgggaggaca
     1861 ccggcagggt ctccggaacc tgatcggcca ttcatgaata aacccttacg gacgctcttg
     1921 cgtaccctgg accgctcacg ggcggtcctt gttcaggctc acgactttcc cgatcatgac
     1981 gcgatcgcct cggcccatgc ccttgcggcc cttctttcgc gcaaggggtt taacgcttcc
     2041 gcctgcgctt cctttcaggg taacagcatt tccgcctcca tcatgataga ccggctcgcc
     2101 ataaccttac acgccgctga acaccttaca gtagacgagg gaacacaaac catactggta
     2161 gatggttcct gttcgggcgg aacggtctcc cgcgtggtgg gaaccttaac cggcattatt
     2221 gaccatcatc ccggcaacat tccccaagac tgtcccttta cggatattcg tgtagaagcc
     2281 ggctcatgct cggccatcat ctggagctac tggcacgagg caggggaaaa cccgga
//
LOCUS       DAOQXQ010000244         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51560_length_2336_cov_2.537484, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000244 DAOQXQ010000000
VERSION     DAOQXQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51560_length_2336_cov_2.537484"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKH81_04835"
     CDS             <1..197
                     /locus_tag="PKH81_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97396.1"
                     /translation="KKAYPELFTIHEPDFIGYQQPIEGLALTIPLSELRSEPKFADLK
                     FTFSENESTQDIQMVYPKKN"
     gene            complement(194..820)
                     /locus_tag="PKH81_04840"
     CDS             complement(194..820)
                     /locus_tag="PKH81_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNQ97397.1"
                     /translation="MKVVQQNCEAEIEVRGSRFLAQIIPIKGQEEARATLRVQKERYA
                     DATHVVHAFISGNSGEVHGCSDDGEPSGTAGRPVLEVLKGSGLTNVLCTVTRWFGGTL
                     LGTGGLVKAYTEAAKAVSQLAISEAEELVELQKFSVILPYDVYERFTRELDEYGARIT
                     ASEFTEAVSLNGMVPAIRAKALSDRVVDISSGRAQCRLSGDARSAINS"
     gene            complement(824..>2336)
                     /locus_tag="PKH81_04845"
     CDS             complement(824..>2336)
                     /locus_tag="PKH81_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ97398.1"
                     /translation="RDDYEDYRSAKKDFFAAYNEYLTGGYDTEIYDTLERFASMTQDD
                     RYRGIRDDIEKNFEVYREDLKVHQNQIETLERICADTFCIIGYSGVGTSDLGVNPFWK
                     LYPNVGTHANIYNTIMSGQYIYALPAWVSWVVALVLCYICAMAHRRIKSLRGRIIFGI
                     ASTTVVFVISAVIFTLFRIYLQTLLPVIAVFMSFILISILRFVFSEQEKSFLRKAFTM
                     YLSSDVVNQIVDDPSLLKLGGQEKQITALFTDIKSFSTLSEKVSPEHLVQILNKYLTV
                     MSDIVLDQKGTIDKYIGDAIVSFFGAPIDLPDHAIRACMAAVRMKEAEVRLNEEMLAS
                     GDTPMPIYTRIGINTGAMVVGNMGTDNKMNYTIMGNDVNLAARLEGVNKQYGTWILVS
                     ESTWNATGGLFLGRKLDRVRVVGIDTPVQLYNIMAVRAEAKGNMVALADRFNFAMDAY
                     RAKKFSDALLLFSKCAELEPEDAATQMYLDRVKDLLKNGVPDNWTDVINMTSK"
BASE COUNT          658 a          593 c          489 g          596 t
ORIGIN      
        1 ggaaaaaagc ctaccccgaa cttttcacga tccatgaacc cgactttatc gggtatcaac
       61 aaccaataga aggccttgca ttgaccattc ctctgtcaga attgcgatct gaacccaagt
      121 ttgccgatct taaatttacc ttttcggaga atgagtcgac tcaagatatt cagatggtat
      181 atcctaaaaa aaactaggaa ttgatggccg atcgcgcatc gccgctaagg cgacactgcg
      241 cacggccaga ggaaatatct acaacacggt cagatagcgc cttcgcgcga atggcaggaa
      301 ccataccatt aagagaaacc gcttcggtaa actccgaggc ggttatcctt gccccatact
      361 catcaagttc acgcgtaaag cgttcgtaca catcataagg aaggataaca gaaaactttt
      421 gaagttcaac aagttcttct gcttcactaa tcgccaattg agagacagcc tttgccgctt
      481 ctgtatacgc tttaacaaga ccaccagtgc ctaaaagagt ccccccaaac caacgtgtta
      541 ccgtacaaag cacattagtt aatccggaac ccttaagtac ctcgagcaca ggccgaccag
      601 cggtaccgga tggctctccg tcatcggagc agccgtgaac ctcgccgctg ttcccgctaa
      661 taaaggcgtg cactacatga gtggcgtcag cataccgctc cttttgaacg cggagcgtgg
      721 cgcgggcttc ttcctgtcct ttgatcggaa tgatctgggc aaggaagcgg gaaccgcgca
      781 cctctatctc cgcttcgcaa ttttgttgaa caaccttcat tctttattta ctcgtcatgt
      841 taattacatc ggtccaatta tccggcacgc cgtttttaag taagtcctta acacgatcca
      901 ggtacatctg cgtggcagcg tcttcaggtt caagctcagc gcatttggaa aacaacaaca
      961 gcgcgtcaga gaatttcttt gcgcgatacg catccatggc aaaattaaac cggtctgcca
     1021 aggcgaccat attccccttc gcttccgcac ggacggccat aatattatac agttgcacag
     1081 gggtatcaat tcccactacg cgaacccgat cgagtttgcg cccaaggaac aagccccctg
     1141 tagcgttcca ggtagattca gagactaaaa tccaggttcc atattgttta ttaacaccct
     1201 caagtcgcgc tgcaaggtta acgtcgtttc ccatgatggt ataattcatt ttattatccg
     1261 tacccatatt gccaacaacc atggctccag tgttaatacc gattcgcgta tatataggca
     1321 tgggcgtatc acccgaggca agcatttcct cgttaaggcg tacttcagct tctttcatac
     1381 gcaccgcggc catgcatgcc cgaatggcgt gatcgggaag gtcaatgggc gctccaaaga
     1441 aggatacaat cgcgtctccg atgtatttgt ctatcgttcc tttttgatca aggacaatat
     1501 cgctcatcac cgttaggtat ttattaagta tttgaacaag atgttcagga cttaccttct
     1561 cggacagagt agaaaagctt tttatatccg tgaacaaggc ggtaatctgt ttttcctgtc
     1621 cgccaagttt taacagggat ggatcatcta caatctgatt tacaacatca gaggatagat
     1681 acatggtaaa agccttgcgc agaaagctct tttcctgctc ggaaaaaaca aacctgagaa
     1741 tcgaaataag aataaacgac ataaacaccg caataactgg caataaggtt tgcaaataaa
     1801 tgcggaatag cgtgaagata acggcgctaa tgacaaacac aaccgttgtg gatgctatac
     1861 caaagatgat gcgtccacga aggcttttta tccgtcgatg tgccatggca cagatatagc
     1921 aaagaacaag agctacaacc catgacaccc aggctggcag tgcataaata tattgaccgg
     1981 acatgatggt attataaata ttcgcgtgtg ttcctacgtt cggatagagt ttccagaaag
     2041 ggttaacccc aagatccgag gttcccacac cgctatagcc gatgatacaa aaagtatctg
     2101 cgcatatacg ctccagggtt tctatctgat tctgatgaac cttgagatcc tcccgatata
     2161 cttcaaagtt tttttcgatg tcatcccgta tcccccgata tcgatcgtct tgagtcattg
     2221 acgcaaaacg ctcaagggta tcatagattt ctgtatcata ccctcccgtc agatactcgt
     2281 tgtatgcggc aaaaaaatcc tttttggcac ttcgatagtc ctcataatca tcacgt
//
LOCUS       DAOQXQ010000245         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51580_length_2335_cov_3.222368, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000245 DAOQXQ010000000
VERSION     DAOQXQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51580_length_2335_cov_3.222368"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKH81_04850"
     CDS             complement(<1..532)
                     /locus_tag="PKH81_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNQ97399.1"
                     /translation="MPIQKNSTCGILTSGGDAPGLNAAIRAVARTAMDVYGIKIIGFE
                     HGYRGLIENKSRVLEPSEFSGILTRGGTILGTSREKPFKDKNWCTETGGPCAVDRIKQ
                     TYRELGLDCLVILGGNGTNTTGAMLAREGLNIIGLPKTIDNDIVETDITFGFHTALTV
                     ATDAIDRLHSTASSHNR"
     gene            612..>2335
                     /locus_tag="PKH81_04855"
     CDS             612..>2335
                     /locus_tag="PKH81_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNQ97400.1"
                     /translation="MKALIRLVLIALFIPAAGFAQEAFLAGTSEGLYRIDKGSSRLIW
                     KQKEVRKIIKLDSSWFFLTAGGVFTSSDLITFESRNNGLPQKVVKIPTTTGKEFAREV
                     QELKDLEIHPTNPRIMVSATRDAVFLTRDGGLTWKSEGLSARTAGVKAVCVLDLPDAR
                     GTTRLTILMTHPIYGVSWKQPDATKPAWVDINKGLEAMPTIRWPDEVADVIAVSRAGS
                     VEVYASQTFMPRLYRLNWAANRFDPIWSGSSVIDSAEGLASGNSSLLFAATNTLMEIP
                     FPVAPTTPPAPVAATAQSIAPWAREFSTVQDILLSAWIPSRASGGRGAISLSELWLLN
                     PSRQIAAYTKTADKKKGTYIPIHQITTDEGLNGHLDTIVKNGLNMLVVDMKDDKGFIR
                     YDAADPLVVQKGKKGKGLELDSFVKKAKEKGIYLVARIVVFKDPQLHRWGSGKYAIWD
                     RREAKPWQGYELVAGEVVPATTDSPTAGTEATAEKVRKYYDEFWVDPYSEEVWQYNVA
                     VAKELIARGFDEIQFDYIRFPTDGVNLNDAQFRWQESGMDKESALMSFLSYARSQITA
                     PISIDIYGA"
BASE COUNT          580 a          602 c          595 g          558 t
ORIGIN      
        1 cgcggttatg acttgaagcg gttgaatgga ggcggtctat cgcatcggta gctacggtaa
       61 gagctgtatg gaagccaaag gtaatatcgg tttccacaat atcgttatct atagttttag
      121 gaagcccgat tatgttaagg ccttcgcgtg caagcatggc gccggttgta ttggtgccgt
      181 taccgccaag gataaccagg cagtcaaggc caagctcacg ataggtttgc tttatccggt
      241 ctaccgcgca gggaccgcct gtttccgtac accagttttt atccttaaac ggtttttccc
      301 gcgaggtacc caaaatagtg ccacccctgg taaggatgcc ggaaaactcc gaaggctcca
      361 atacacggct cttgttttcg atcaggcccc gatacccgtg ctcaaagccg ataatcttga
      421 ttccatacac atccatggcg gtgcgcgcta ccgctctaat cgcggcgttt agtccaggag
      481 cgtcgccgcc ggaggtcagg ataccgcagg tactgttctt ctgtatgggc attgatagcc
      541 tccctttgtg ttcgattata acggtatcca agccttttgt aacagaagtt tttatgttat
      601 cctgctgata catgaaagca cttatccgcc ttgtacttat tgctctgttc atccctgctg
      661 ccggttttgc ccaagaggcc tttttagccg gcacaagcga aggtctttac cggatagata
      721 aaggaagctc ccgcctcatc tggaaacaaa aagaagtccg taaaattatc aagcttgatt
      781 catcctggtt ttttctcact gccgggggtg tgtttaccag tagcgatttg attacctttg
      841 aaagccgtaa caatggtctt ccacagaagg tagtcaagat tcccaccact acgggcaagg
      901 aatttgcccg cgaggtacag gaacttaagg acctggagat tcatccaaca aacccgcgta
      961 tcatggtgag cgcgacacgg gatgctgtgt ttcttacacg ggatggcggg cttacctgga
     1021 aaagcgaagg gctcagcgcg cgaacggctg gagtaaaggc cgtatgtgtt ctggatcttc
     1081 cggatgcgcg agggaccact cgattaacga ttctgatgac ccatcccatc tatggggttt
     1141 cctggaagca accagatgcg actaaaccag cctgggtaga tatcaacaag ggacttgagg
     1201 ccatgcccac cattcgctgg ccagacgagg tcgccgatgt aattgccgta tcccgcgcag
     1261 ggtctgtgga agtctatgct tcgcaaacct ttatgccgcg cctataccgc cttaactggg
     1321 ccgccaaccg ctttgatcca atctggtctg ggtcctcggt aatcgattca gctgaaggtc
     1381 ttgcatcagg aaattcatcg cttctttttg ccgcgaccaa caccctgatg gaaatacctt
     1441 ttcctgtagc accaacgacg ccccctgcgc ccgtagcagc tacagcgcag tcaattgcgc
     1501 cctgggcacg cgagttctcc acggttcagg atatactttt atccgcatgg attccctcgc
     1561 gcgcaagcgg tggccgtgga gctatatctt taagcgagct gtggcttctt aacccctccc
     1621 gacaaatagc ggcgtataca aagaccgcgg acaagaaaaa aggaacctat atccccatcc
     1681 atcaaattac caccgatgag gggctaaacg gacacttgga cactattgtt aaaaatggtt
     1741 taaacatgct cgtcgtggac atgaaagacg acaagggctt tatccgctac gatgccgccg
     1801 atccccttgt ggttcaaaaa ggaaaaaaag gaaagggcct tgagcttgac tcctttgtca
     1861 aaaaggcaaa agaaaaaggg atctatttag ttgcccgtat cgttgtgttt aaggatccgc
     1921 aactgcaccg atggggatcc ggaaaatacg caatttggga tcggcgagaa gccaagccct
     1981 ggcaagggta tgaattagtc gcgggcgagg ttgtacccgc caccaccgat agcccaaccg
     2041 caggaaccga ggcaacagcg gaaaaagttc gtaagtatta cgacgagttt tgggtagacc
     2101 cttacagcga agaggtgtgg cagtataacg tcgccgttgc caaagagctt atcgcgcgcg
     2161 gctttgacga aattcaattt gattatatcc gcttccccac cgacggggtg aatctgaacg
     2221 acgcgcagtt ccgatggcaa gaaagtggca tggacaagga aagcgccctg atgtcctttt
     2281 tgtcttacgc ccgatcacag attacagcgc caatctcaat agacatatac ggcgc
//
LOCUS       DAOQXQ010000246         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51691_length_2332_cov_2.941590, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000246 DAOQXQ010000000
VERSION     DAOQXQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51691_length_2332_cov_2.941590"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PKH81_04860"
     CDS             <1..1351
                     /locus_tag="PKH81_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97401.1"
                     /translation="ELVDPLLVPVAEKLATSMESGERFTREYRVFLNSGVLCILCDFY
                     CLRDSRLGRVFVISLEDRSREDEIKQKLSTQLEETHRYALSKDNFFANMSHEIRTPIN
                     AILGITYFARSHCSDGKCLEYISKIENASELLLSVVNDILDFSKMQEHKFSLNPENFN
                     LFDLQKILTDLFALKAIQKSLELRIEFDCPQRFYVYGDQFRLTQIFMNLVSNALKFTE
                     NGFIKVSLNHENIGDDIILRCTVRDSGLGIVEKDLDKIFTDFEQFGQVLVKTHEGTGL
                     GLAICKRLVELMHGVIWVDSLPGQGSSFHFVVVLKKPTELTGEELSMNLPRIERHSGR
                     ILVVEDNEINGEIVETLLAREGLEVLRATNGLEAIDICREKGPDAFDLVLMDIHMPRM
                     NGYDAARVLKQEIGIRCPIIAVTATSENTRVLEENGDIIDGYIIKPYDPEVFHELFS"
     gene            1363..1734
                     /locus_tag="PKH81_04865"
     CDS             1363..1734
                     /locus_tag="PKH81_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNQ97402.1"
                     /translation="MDTFTLVRTEHLNHNKKLFGGQLLKWVDEFAWLAAARDYSGSVL
                     VTRAMDNSEFRFAVPNGSILRFHIECMHEGTTSVLYSVDVFADIPGESVEKLIFTNKV
                     TFVAIDEAGNKTPLKKRGCGA"
     gene            1738..>2332
                     /locus_tag="PKH81_04870"
     CDS             1738..>2332
                     /locus_tag="PKH81_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ97403.1"
                     /translation="MNVLSTAHRLSGAITVPGSKSHTIRAGILASLGSGTSYIRNPLE
                     SADCLSCLDVIEAFGACVRREKGLWTVTAPEGGLRLPARVIDAQNSGSVLYFMSAIAA
                     TLPGWTVMTGDESLCTRPIDTLLQAIRDLGGKGFTTRPGIDAPPAVIGGPFVAGAVEL
                     EGHLSQPVSGLMMAAARISGTTVIRLRDPKETPFLKMT"
BASE COUNT          556 a          536 c          611 g          629 t
ORIGIN      
        1 cgagctggtt gatccgctcc tggttcccgt ggcagaaaag cttgcaacct ccatggagag
       61 cggggagcgc tttacacggg aatatcgcgt gtttttgaac tcaggcgtct tgtgcatcct
      121 gtgcgacttt tattgcttgc gggatagccg cctgggcagg gtgtttgtaa tatctcttga
      181 ggatcgcagc cgcgaagacg agattaagca aaagctttcc acccagctag aagaaacgca
      241 tcgatatgcc ctttccaagg ataatttttt cgcgaatatg agccatgaaa tccgcacgcc
      301 catcaacgct attttaggta ttacctattt tgcccgcagt cattgctctg atggtaagtg
      361 ccttgagtat ataagcaaaa tagaaaatgc ctccgaactt ttgctctcgg ttgttaacga
      421 tatccttgat ttttccaaga tgcaggagca caagttttcg ttgaacccgg agaattttaa
      481 cctctttgac cttcaaaaga ttctaaccga tctttttgcc cttaaggcaa ttcagaagtc
      541 cctggaatta cgtattgaat ttgattgtcc ccaacgattt tatgtgtatg gagaccagtt
      601 ccgcctgact cagattttta tgaacctggt aagcaacgcg ctcaagttta cggagaacgg
      661 gtttatcaag gtttcgctca atcacgaaaa tattggcgat gatattatcc ttcgctgtac
      721 ggtgcgggat tcaggtctgg gaatcgtaga aaaggatttg gacaagattt ttaccgattt
      781 tgaacagttc ggtcaggtac tggttaagac tcacgaggga acgggccttg gtttggctat
      841 ttgtaaacgt ttggtggagt tgatgcacgg ggttatctgg gtggatagcc tacctggtca
      901 gggttcgtcc tttcattttg tagtcgtact taaaaaaccg actgaactaa ccggagagga
      961 actgtccatg aacttaccgc gaattgagcg gcatagcggg cggatactcg tggtggaaga
     1021 taacgaaatt aacggagaaa tcgttgaaac cttgttagcc cgggaagggc ttgaggtttt
     1081 gcgagccacc aatgggctcg aggcaattga tatctgtcgc gaaaaaggcc cggatgcctt
     1141 tgatttggta ctcatggata ttcacatgcc ccgaatgaac gggtatgatg cagcgagggt
     1201 gttaaagcaa gaaatcggta tacgctgtcc cattattgcg gttaccgcta caagcgaaaa
     1261 caccagagtc cttgaggaga acggggatat tatagacggc tatatcatta agccctatga
     1321 cccggaagtt tttcacgaat tgttttctta aggggaatta ttatggacac ctttactctg
     1381 gtgcgaaccg agcaccttaa tcataacaag aagctttttg gcgggcagct gttaaagtgg
     1441 gtagacgagt ttgcatggct tgccgccgcg cgggactatt ccggctcagt tctggtgacc
     1501 cgcgccatgg ataacagcga gttccgcttt gctgttccca atggatcaat ccttcgcttt
     1561 catatcgaat gtatgcatga gggcacaacc tctgttttat attcggttga cgtctttgcc
     1621 gacattcccg gcgagagtgt ggaaaagctg atatttacca ataaggttac ctttgtcgct
     1681 atagacgaag caggaaataa aacccccctt aaaaaaaggg gctgtggagc ttaaaccatg
     1741 aacgttcttt caaccgcaca ccgattgtcc ggggcaatca ctgtacccgg gtctaaaagt
     1801 cataccatca gggctggcat tctcgcttcc cttgggtcgg gaacctcgta tatcagaaat
     1861 ccgctggaaa gcgcggactg tctttcatgt ctggatgtta tagaagcctt tggcgcctgt
     1921 gtgcgtcggg aaaagggctt gtggacggta accgcacccg aaggtggctt gcgccttccc
     1981 gcacgagtca tcgatgccca gaattccgga tcggtactct attttatgtc ggccatagcg
     2041 gcgacgcttc ctggctggac ggttatgacc ggcgatgaat ccttgtgcac tcgccccatc
     2101 gataccctgc ttcaggctat ccgtgatttg ggcggcaagg gctttactac ccggcccggt
     2161 attgatgccc cgcctgcggt tattggcggg ccctttgttg ccggagcggt agagcttgaa
     2221 gggcatttgt ctcaaccggt aagcggactg atgatggctg ccgccaggat ttccgggact
     2281 acggtaatcc gtctgcgtga tcctaaagaa accccctttc tcaagatgac gg
//
LOCUS       DAOQXQ010000247         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51812_length_2329_cov_2.343448, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000247 DAOQXQ010000000
VERSION     DAOQXQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51812_length_2329_cov_2.343448"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKH81_04875"
     CDS             <1..1138
                     /locus_tag="PKH81_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ97404.1"
                     /translation="WLKAYLAHGCYWHRCSSCDVTLDYIQSYLPVDVPALYAALSAQR
                     GPASSGIHLVDEAAPPRLLRDFARQNRLATADGTSLMPFWGNIRFEKSFTRDLAEYLA
                     SGGLLAVSGGIEIACPSGFKAVDKGIDLENLVATCAAFKESGVLVHAYLMYGYWNESD
                     ADLIDSMEVMRQLFAAGLVDSAFWHKFVLTRHSRAYTEWKQGSGDRSLVPCDDKASDF
                     ADNDLDFTGSGESSRYTPYLDAALAAWMAGEDLDRRVSSWFPFRLPQPRIAGNVIEEY
                     IRGYERRRDEAWRESADSRSQYEWTASDPVSFLGEDGSARLVWWHLGEEIEIDLDKKT
                     SEKVVELLERAQAGNALWGGEFEGLDRRLFSLLRSAGLVRISPL"
     gene            complement(1147..2115)
                     /locus_tag="PKH81_04880"
     CDS             complement(1147..2115)
                     /locus_tag="PKH81_04880"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HNQ97405.1"
                     /translation="MRFHLAFTAILLAFLASVTAEDLAFEYSLSMGQLSGGAHELVYY
                     SGDTILSELYWPLDGAIVFSASARAVFPGGFALSLGIDSALSGDTGAMTDRDYLNLPT
                     DGSLTHFSSHETNLDRLLGFSLCLSRRFSSPLHGLASPQRIVFEGALGIRITSTHWTA
                     SDGYTQYGPWTGEDWTPWRDNLPREDLSGEVITWEQELVSLFAMITVEVPLGRLFLLR
                     GSVLASPLNISHAKDHHLLTRMYFIDRMSGGLLLIPELTLAWRPAPRWEAFAAISWLT
                     LSPLRGSTEIRDEQGYIISGSDGSSGTDMETTTIRMGLTRRLSARP"
BASE COUNT          548 a          577 c          675 g          529 t
ORIGIN      
        1 gtggcttaag gcctatcttg cccatggctg ttactggcat cgatgctcat cttgcgatgt
       61 tacgctcgac tatatacaaa gctatcttcc tgtagatgta ccggctctgt atgccgcgtt
      121 atccgcgcag cgagggcccg cttcttcggg gattcatcta gtggacgagg cagctcctcc
      181 ccgactcttg cgcgattttg cccgccaaaa tcgtcttgca acagcggacg gaacatcctt
      241 aatgcccttt tggggaaaca tccggttcga aaaatccttt acccgggacc tggccgaata
      301 ccttgcctca ggcggcttgt tggctgtctc tggcggtatc gagatagcct gcccttcggg
      361 ctttaaggcg gtagataagg gcatagacct ggaaaacctg gtagcaacct gtgcggcctt
      421 taaggagtcg ggcgtgttgg tacatgcgta cctgatgtac ggctattgga acgagagcga
      481 cgctgacctt atcgattcta tggaagtgat gcgccagctc tttgccgcgg gtctggtgga
      541 ctcggcattt tggcataagt ttgtgttaac ccgccattca cgcgcgtata ccgaatggaa
      601 gcaaggctct ggcgatcgga gtcttgtccc ctgcgacgac aaggcaagcg attttgctga
      661 taatgatctt gattttaccg gttccgggga atccagcagg tacaccccgt atctggatgc
      721 ggcccttgct gcctggatgg cgggcgagga cttggaccgt cgtgtgtctt cctggttccc
      781 ctttcgccta ccacagccgc gcatcgcggg caacgtgatc gaggagtata ttcgcgggta
      841 tgaacggcgg cgggacgagg catggagaga gagcgcggat tcccgttcgc agtacgaatg
      901 gaccgcttcc gatccggtat catttttggg ggaggatggt tctgctcgcc ttgtatggtg
      961 gcaccttggg gaagagatag agattgatct tgataaaaaa acgtcggaga aagtcgtaga
     1021 acttcttgag cgcgctcagg cgggcaacgc gttatggggc ggggaatttg agggtttgga
     1081 tcgtcggttg ttttcgctat tacgaagtgc aggtcttgtt cggatctctc cgttgtagga
     1141 cccgcctcag gggcgagcgg acagtcggcg ggtaaggccc atgcggatgg tggtggtttc
     1201 catgtcggta ccgcttgaac catcgctgcc gctaataata tatccctgtt catcgcgaat
     1261 ctcggtgctt cctctaagcg gggaaagtgt aagccaggag atagcggcaa aggcctccca
     1321 gcggggcgcg ggtctccatg caagagtcag ttcgggtatc agcaaaagtc ccccggacat
     1381 gcggtctata aaatacattc tggttaacag gtgatggtcc ttggcgtgcg aaatgttgag
     1441 cggggaggca aggacggaac cacgcagcaa aaaaagccgg ccaagtggca cttctaccgt
     1501 aatcatcgcg aataatgaga caagctcttg ttcccaggtt attacttctc cagaaagatc
     1561 ttccctgggc aggttatccc tccagggagt ccagtcctct ccggtccagg gaccgtattg
     1621 ggtgtatcca tcgctcgccg tccagtgggt agaggtaatg cggatgccaa gggctccttc
     1681 aaagacaatg cgttgtgggg aagcaagccc gtgtaaagga gacgaaaaac gacgggagag
     1741 gcaaagtgaa aaccccagca agcggtccag gtttgtctcg tggcttgaaa aatgggtaag
     1801 agaaccatca gtgggaagat taagataatc ccggtcggtc atcgcgccag tatctccgct
     1861 cagggcacta tcgatgccaa gggacagggc aaaacctccg gggaacacag ccctggcaga
     1921 ggcggaaaaa acaatggcgc cgtcaagcgg ccaatagagc tcgctcaaga tcgtgtcgcc
     1981 gctatagtac acaagttcat gagcgccgcc cgaaagctgc cccatggaaa gagaatattc
     2041 aaaggcaaga tcctcagcgg tcacagaagc caggaaggcg aggagtatgg ctgtaaaagc
     2101 aagatgaaag cgcatatcta ttattctaac acaaagcgct tgccgatttg cagcaaatct
     2161 cgttaccttt ggtacaggcg catgccgcct tgaacaaaaa tccattccaa cgcaagcgcg
     2221 ggagatgtac agaacatggc aacgtatcag tttcaaaccg aagtcaatca attattgacc
     2281 cttattattc attccttgta ttcaaacaag gagatattcc tgcgggaaa
//
LOCUS       DAOQXQ010000248         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_51863_length_2327_cov_3.125440, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000248 DAOQXQ010000000
VERSION     DAOQXQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51863_length_2327_cov_3.125440"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKH81_04885"
     CDS             complement(<1..238)
                     /locus_tag="PKH81_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97406.1"
                     /translation="MVTDDDLHYMRLRFTEYAETDSQSDPDKADRGIMPSTDGQTRLA
                     QLLSTELTTYGLTDNTVDQNGYLRAVIPASKGMES"
     gene            323..1204
                     /locus_tag="PKH81_04890"
     CDS             323..1204
                     /locus_tag="PKH81_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNQ97407.1"
                     /translation="MSLIPAYPEFAPLSLEMAQEISPHMVKLPDGISEFSFTGLYLFR
                     HRYNYRASISGDLLILSGERDGSRFFITPCCSTGKEIVEKLFSDHDYWKLISPAFLEK
                     NSQWMRDAGYEIQEDRNNFDYLYLRDDLAHLSGKKFHKKKNHVNAFLSSYPSWTIKDL
                     NGETRKDALDVLEAWVLHEEEPQNTDYEAAHEVLSLMDHFPMTGQVLYVDGTPIAWTL
                     AETLGDGLISAVHFEKARTEYRGSYQFINYAYARSLPDSIKYINREQDLGDEGMRQAK
                     MTYRPSGFVMKYRVNRP"
     gene            1326..>2327
                     /locus_tag="PKH81_04895"
     CDS             1326..>2327
                     /locus_tag="PKH81_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ97408.1"
                     /translation="MKLVSTRNSSHIVSFREAVLDCIPPDGGLYVPASEEDLRPWVMY
                     MDETTSFASIAGTLTSALLREEFSPVVCERIAGTAFAHSSPLLRQLDDRFYSLELYHG
                     PTGCHKDFAFSWFASALEHLLVMEDREATVLAATGGVTGRSLAAAFSGKKRLKLVLAY
                     AKGTLAGLDPESLAWNGGNIYPVEVEGDLKSAQELVRQIYSDPDLVSRFGLTLANTMN
                     IGRLLPQVFFYMFAFTRLRKKVLGDIFYAVPSGNFGNLVAGLYAWKFSLPVNGFITDA
                     TAALSCDATGQCMCMDSIVPLANRGAADPASPSNLERLEQVFQVNPMILRGLVFPSEV
                     "
BASE COUNT          534 a          534 c          582 g          677 t
ORIGIN      
        1 cactttccat gcccttggat gcggggatca ctgcccttag gtagccattt tgatcgactg
       61 tgttgtcggt taacccgtag gttgtaagct ccgtcgaaag tagctgtgcc agacgggtct
      121 ggccgtctgt agaaggcatt atacctctgt ctgccttatc tggatcgctt tggctatctg
      181 tttctgcgta ttctgtgaat cgtaatcgca tgtagtgtaa atcatcatcg gttaccatgg
      241 gaaccagagt acgctatttg gaatgaccag tcaatcttgg acttcttgcc gaatacctgt
      301 caaaacagta ccttacatat atatgagctt gatacccgcc tatcctgagt ttgctcccct
      361 aagtcttgaa atggcccaag aaatctctcc gcatatggtt aagcttcccg acggaatttc
      421 tgagttctcc tttaccggat tatatttgtt ccgccatcgg tataactatc gagcttccat
      481 tagtggagat ttattgatac tttcgggaga gcgggatggt tcgcgctttt ttattacccc
      541 ctgttgttca accggtaagg agatagtgga aaagttgttc tctgaccatg attactggaa
      601 actgatatct ccggcatttt tggaaaaaaa cagccagtgg atgcgagatg caggctacga
      661 gattcaggaa gatcgcaata attttgacta tttgtatctt cgggatgatt tggcacatct
      721 ttcgggaaag aaatttcaca aaaagaaaaa ccatgttaat gcctttcttt cctcctatcc
      781 ttcatggacg ataaaggatc ttaatggaga aactcgcaag gatgccctgg atgtgcttga
      841 ggcatgggtt ttacacgaag aggaaccgca aaataccgat tatgaggctg cccacgaggt
      901 attgtctctt atggatcatt ttcccatgac cggccaggtg ttatacgtgg acggtacgcc
      961 tattgcctgg acccttgccg aaacccttgg tgatggatta atctccgctg tacattttga
     1021 aaaggcgaga acagagtatc gtggtagcta tcaatttatc aattatgcct atgcccgctc
     1081 tcttccggat tccatcaaat acatcaatcg tgagcaagac ttgggtgatg aagggatgcg
     1141 acaggcaaag atgacctatc gaccttcggg ttttgtcatg aaataccggg tcaatcgtcc
     1201 ctgaaccagg atctggcgaa aattcgatgc aggatggcat accccgttcc ccaaatttcc
     1261 ctcttgtggc atactgcccg ccgttagtgt tatactccta agaaggacaa aaccgggagg
     1321 ttttcatgaa gttggtcagc acccgaaaca gttctcatat tgtcagcttc cgggaagctg
     1381 tgcttgattg tattccgccg gatgggggct tgtatgttcc tgcaagtgaa gaagatttac
     1441 gtccctgggt aatgtatatg gacgaaacga cttcctttgc ctctattgcc ggaaccctta
     1501 cctcggccct gttgcgggaa gagtttagcc ctgttgtgtg tgaacgaatc gccggaacag
     1561 cctttgccca ttccagtccc cttcttcggc agcttgacga tcggttttat tctctggagc
     1621 tataccacgg tcctaccgga tgtcataaag attttgcttt ttcctggttc gcttctgcat
     1681 tagagcattt gcttgttatg gaagacagag aggccactgt attggctgca acaggcgggg
     1741 taactggccg cagccttgcg gccgcctttt ctggcaagaa acgccttaag ctggtcctgg
     1801 cctatgcaaa ggggaccctt gcaggtcttg atcctgaaag ccttgcctgg aatggtggaa
     1861 atatctatcc ggtagaggtt gagggagacc taaagagcgc acaagaattg gttcggcaga
     1921 tatacagcga tcctgatttg gtctcccgct ttgggcttac gcttgccaat acaatgaata
     1981 ttggtcgctt attacctcag gtgttttttt acatgtttgc ctttacgcgt ctacgcaaaa
     2041 aggttctggg cgacattttt tacgcggtcc cctccgggaa ttttggtaac ctggtagccg
     2101 gattatatgc atggaaattt tctttaccgg taaacgggtt tatcaccgat gcaacagcgg
     2161 ccttgtcctg tgatgcgacg gggcaatgca tgtgtatgga ctcgattgtt cctttagcga
     2221 atcggggggc cgcggacccg gcaagtccgt cgaatcttga gcggttggag caggtgtttc
     2281 aggttaatcc catgatcctt cggggcttgg tgtttccctc agaggta
//
LOCUS       DAOQXQ010000249         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_52089_length_2321_cov_2.621801, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000249 DAOQXQ010000000
VERSION     DAOQXQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52089_length_2321_cov_2.621801"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKH81_04900"
     CDS             complement(<1..263)
                     /locus_tag="PKH81_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97409.1"
                     /translation="MFKKPNQIHGPVLLAGDSPYFKIIASELGKTDLLQNRPIITTRP
                     KREELVQAHAEKASLIIIEGVDVARELLDDPSMKDSQADILATS"
     gene            complement(269..1528)
                     /locus_tag="PKH81_04905"
     CDS             complement(269..1528)
                     /locus_tag="PKH81_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ97410.1"
                     /translation="MSEHTLTLALILLLLALSAFFSAAETAVTSITRTGLRRIGSQDN
                     SAALALRRLLERRSRIITTILVGNNIVNIWASSLATALALEFTGESGILISTGVMTLC
                     ILIFGEITPKNLASKNPEGLTLAISPLLRSIEILLFPITSFFSLINRVALAISAFFGK
                     PGESRLTEDELRMMVQAGHRDGALEEGERRLLERAVAFTGKRVREIMTARTAMAALSV
                     DTPIDEVIAFFREREFSRLPVYREDLDTIVGIVHYKDILFVEGDASFTLADLIREPLV
                     VPETQRVKELFTALNKAGQNMAIVVDEHGSTAGLVTMDDALSSVIGDIHDEYDEANDH
                     PRQRVRIIGKEKIIVPGDLLLDDLNALLKTSFDSWYYETVGGLILETAGRLPERGEVF
                     RLGKAQFKVLSRAQRTITRVEIRLDKE"
     gene            complement(1525..>2321)
                     /locus_tag="PKH81_04910"
     CDS             complement(1525..>2321)
                     /locus_tag="PKH81_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ97411.1"
                     /translation="AAAPAQKGHRVTEEDLRALIEVGEEDGLLASADGRIMRRILDST
                     DMSARDIMTARTDIIALPLDATRQEILDTSRESHFSRIPVYQDSLDNIRGIVHIKDLF
                     LSPTGARASDLMKEAVFAISSLKIPSLQAILQKNSQNMAIVIDEFGGTAGLVTSEDVF
                     EKIFGSIRDEHDDEEDGSSLSSPDIPECCFDVDASIHLADLSERLSIPLESTFYDTLA
                     GFMMEQTGDIPQQGQRLLYQGWLFTVIEKTGNRLDTVRVEKEEGLV"
BASE COUNT          558 a          582 c          584 g          597 t
ORIGIN      
        1 gaggttgcaa ggatgtccgc ttggctatcc ttcatggacg ggtcatccag gagttcccgt
       61 gccacatcca cgccttcgat aataatgagc gatgcctttt cggcatgggc ttggacaagc
      121 tcttcgcgtt tgggtcgagt tgtaatgatt ggtctgtttt gcaaaaggtc agttttgcct
      181 agctcgctcg cgataatttt aaaatacgga ctatccccgg ctagcaagac cggcccatgg
      241 atttgattgg gttttttgaa catgatcgtt actccttgtc caggcgtatc tctacccgtg
      301 taattgttct ctgagcgcgc gaaagcacct taaactgagc cttccctaat cggaaaacct
      361 ctcccctttc gggtaggcga ccggccgttt ccaggattaa gccaccaaca gtttcataat
      421 accacgagtc aaaggaagtt ttgagcaggg cgttcaagtc gtccagcaag agatcaccgg
      481 gaactatgat cttttcttta ccgataattc tgaccctttg tcggggatga tcgtttgcct
      541 cgtcatattc gtcgtggatg tctccaatta ctgacgaaag ggcatcgtcc atggtaacaa
      601 gacctgcagt agatccgtgt tcatctacca caatcgccat gttttgcccc gccttattaa
      661 gcgcggtaaa aagttccttt acccgctgtg tttccggtac aaccaagggt tcgcgaataa
      721 ggtctgccag agtaaaagag gcatcccctt ccacaaagag aatatccttg tagtgaacaa
      781 ttccaacgat ggtatcaagg tcctcgcggt atacggggag gcgggaaaac tcccgttctc
      841 taaaaaaggc gataacctcg tcaatcggtg tgtctacgga cagggctgcc atggctgtgc
      901 gtgcggtcat gatttcccgc acccgtttcc cggtaaaggc caccgctcgc tctaaaagac
      961 ggcgctctcc ttcttcaagg gccccgtccc gatggcctgc ctggaccatc atcctcagtt
     1021 catcttcggt taagcggctt tcccctggtt tcccaaaaaa agccgagata gcaagagcta
     1081 cgcgattgat aagcgaaaaa aacgaggtaa tcgggaagag gagaatctca atacttcgca
     1141 ggagcggaga gatggcgagg gtgagtcctt ccggattttt tgaggcaaga ttctttgggg
     1201 ttatttctcc aaagatcagg atgcacagcg tcatgactcc ggttgagatt agtatgccac
     1261 tttctccggt aaactcaagg gcaagggcgg ttgcaagact ggatgcccag atattgacga
     1321 tattgtttcc cacaagaatg gttgtgataa ttcggcttct tcgctccaga agtctcctga
     1381 gcgcgagtgc ggccgaattg tcctggcttc cgattcgcct gagacctgtt ctggtaatgg
     1441 aggttactgc ggtttcggcc gcggaaaaaa aagccgaaag tgcgagtaac aaaagtataa
     1501 gcgcgagggt aagtgtgtgc tcgctcatac caagccctct tccttctcaa cccgtacggt
     1561 atcaagtcta ttccccgttt tttcgataac cgtaaaaagc catccctgat ataaaagtct
     1621 ttgtccctgc tgtggtatat cccctgtctg ttccatcata aaaccggcca gggtatcgta
     1681 aaaggtagat tcaaggggaa tggaaaggcg ctctgaaaga tcggcaagat ggatgcttgc
     1741 atctacgtca aaacaacatt caggtatgtc cggggaagac aaggatgagc cgtcttcttc
     1801 atcatcgtgt tcatcccgga tactgccgaa tatcttttca aaaacatcct cactggttac
     1861 caggccggcc gtacccccga attcgtcgat cacgattgcc atgttttgac tgtttttttg
     1921 gaggatagcc tgaagcgaag gaatcttgag ggaactgatg gcaaaaaccg cttctttcat
     1981 aagatcgctt gcccgtgccc cggttggcga cagaaacaaa tcttttatat ggacaatgcc
     2041 gcgaatgtta tcaaggctat cctggtacac gggaatgcgg gaaaaatgtg attctcggga
     2101 ggtatccaga atttcttgtc gggtagcgtc cagaggaagg gcgataatat ctgtgcgcgc
     2161 cgtcatgata tcccgggcgc tcatgtccgt tgaatcaagg atccttcgca taatccgccc
     2221 atcagcggaa gccaaaagcc cgtcttcttc tccaacctca ataagggctc gcaggtcttc
     2281 ctcggtaacc cggtgtccct tttgagcggg agctgcagcg g
//
LOCUS       DAOQXQ010000250         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_52091_length_2321_cov_2.514563, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000250 DAOQXQ010000000
VERSION     DAOQXQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52091_length_2321_cov_2.514563"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PKH81_04915"
     CDS             complement(<1..1288)
                     /locus_tag="PKH81_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ97412.1"
                     /translation="MTECCHDVAIVGAGVSGAAIARLLSRYQLKVALLEKEVDVSFGT
                     SKANSGIIHAGFHHAGSLLKTRLELEGLAQFDQLQRELSFPFRRCGILVVALHPDEMG
                     MVEHLWKQGLANGVSGLELCSRERLLALESKLADDVVGGLYAPNGGIIEPYRFVFSLV
                     ENAVLNGVSLRTSFKVVNAIHDGLSWSITSSSGEVLRARYVVNAAGLFADEVSRIFGA
                     EDFTIQGRKGEYFLLDRQAKAHPSRVVFPVPSSISKGMLVIPTVEGTTLIGPTADACP
                     DKEDLATDSTRLERILSSARNLIPSVSERDVITSFAGIRPVYGDDFYIAQSRKVPNLI
                     QVAGIQSPGLTASPAVACMVRDLLVRASLQLTENPAYISHLDPQVRVRDLSLEERQTL
                     AKENPAWAQIVCRCEQISEAEIIEAVRLDHPVSVVIQ"
     gene            complement(1269..>2321)
                     /locus_tag="PKH81_04920"
     CDS             complement(1269..>2321)
                     /locus_tag="PKH81_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97413.1"
                     /translation="VSEDEELPEDDLETLDDLVGFEEPIEIPSSSPMDEPVGEEEILP
                     DSTYNSPDDGAEGESILEEGEEDFSEPSSSPAYEDEPSWNDEIQPQDDGMDYLDMPPE
                     PAQPRAEPPRSEPQRAEPPRTEPQRAEPPGPEPQRAEPPRAEPPQAEPPRYPPEPFPP
                     QYPPLNREPPDPEPAAPAKAPSFQQEQSEDLDLSQPDDGESAGSACSENQLVRRHGLP
                     MGTPVLSPPKLDDVLEEIPSVDILALFSFLKSITMSLPEEILSEYLLSDERVQMEYII
                     DRLSGRPGLKHLAALRRQTGSVLPTDPEVLIQTGIRESFEYLENLVESLPDQGFVVTI
                     RNRLEDLRTRFDRVLP"
BASE COUNT          583 a          617 c          593 g          528 t
ORIGIN      
        1 tctgaataac aaccgatacc ggatgatcca gtctgaccgc ttcaataatt tctgcctcgc
       61 taatttgttc gcatctacag actatctgtg cccatgcagg attttccttc gcaagcgttt
      121 gacgctcttc cagggaaaga tcccgcacac gaacctgggg atcgagatga ctgatgtacg
      181 cggggttttc ggttaactga agggatgcgc gtacgagtag atcgcgaacc atgcaggcta
      241 ccgcaggaga agcggttaag cccggggact ggataccggc tacctgaata agattaggca
      301 ccttgcggga ttgagcgatg taaaaatcat ctccatacac tggacgaatg cccgcaaagg
      361 aggtgataac atcgcgctcg gaaaccgagg gaatcaggtt tcgggccgaa gataggatgc
      421 gctcaagcct ggttgaatcg gtggcaagat cctctttgtc cgggcaggca tcggccgttg
      481 gcccgataag ggtagttcct tctaccgttg gaataacgag catgcctttg gaaatagatg
      541 agggaaccgg aaacacaact cgcgaaggat gtgcttttgc ctggcgatca agaagaaaat
      601 actccccctt tcgcccctgt atcgtaaagt cctccgcgcc aaagatgcgg gaaacctcgt
      661 cggcaaacag tcctgcagcg ttaaccacat accgggcacg aagcacttcc ccgcttgatg
      721 aagtaatact ccaggaaagc ccgtcatgaa tcgcgttaac aaccttgaat gaagtgcgaa
      781 gggatacccc gttaagaacc gcgttttcca caagggaaaa aacaaaacgg tacggctcaa
      841 tgatgccccc gtttggcgca tacaagccgc cgactacatc atctgcgagc ttactctcca
      901 aagcaagaag ccgttccctg gagcataact ccaaaccgga gaccccgttg gcaagcccct
      961 gcttccacag gtgttccacc atccccatct cgtcaggatg taaagctaca accagaatac
     1021 cacagcgccg aaagggaaag gaaagctctc gctgtaattg atcaaactgg gcaagtcctt
     1081 caagctcaag ccgagttttt aataatgaac ctgcgtgatg aaatcccgca tggatgatac
     1141 cggagtttgc ctttgaggtt ccaaaagata catccacctc tttttctaaa agcgctacct
     1201 taagttgata gcgggaaagg aggcgagcga tagcagcgcc ggaaacccca gcgcctacta
     1261 tcgcaacatc atggcaacac tcggtcaaaa cgagtcctta aatcttcaag gcgattgcgg
     1321 atggtaacca caaatccctg gtctggcaga gactcaacca gattctccaa atattcaaac
     1381 gactcgcgta tccccgtttg aatcaatact tccgggtcgg tggggagcac cgaaccagtt
     1441 tgcctgcgca aggcggcgag gtgtttcaag cccggcctgc ccgacaaccg atcaattata
     1501 tattccatct gaacacgttc gtcgcttaat aagtattccg aaaggatctc ttccggcaag
     1561 ctcattgtta tagacttaag aaaggaaaac agggcaagaa tgtccacgct tggaatttct
     1621 tcaagtacat catcgagctt gggcgggctt aacaccggag ttcccatggg tagaccatga
     1681 cgcctgacta actgattctc tgaacacgcc gatccagcgg attccccatc atcaggttga
     1741 gagaggtcta aatcctcact ctgttcttgt tgaaatgacg gggcttttgc gggagcagcc
     1801 ggttcgggat ctggcggttc ccggttcaag ggaggatact gcggagggaa tggttcaggc
     1861 ggataccgag gaggctcggc ttgtggcggc tcggcccgtg gcggttcagc tctctgtggt
     1921 tcaggccccg gcggttcagc tctctgtggt tcagtccgcg gcggttcggc tctctgtggc
     1981 tcagagcgcg gcggctcagc gcgcggctga gctggttcag gcggcatatc aagataatcc
     2041 ataccgtcat cttgaggttg aatctcgtca ttccaggaag gctcatcctc atacgccggt
     2101 gaagaagaag gttcactaaa atcctcttcc ccttcttcta atatactctc cccttcagca
     2161 ccgtcatccg ggctattata cgttgaatcg ggaagtatct cttcttcgcc aacaggttca
     2221 tccattggtg aggaagatgg aatttctatg ggttcttcaa aaccgactaa atcgtccagg
     2281 gtttccaggt catcttccgg aagctcttcg tcctcggaaa c
//
LOCUS       DAOQXQ010000251         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_52189_length_2318_cov_2.559434, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000251 DAOQXQ010000000
VERSION     DAOQXQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52189_length_2318_cov_2.559434"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKH81_04925"
     CDS             complement(<1..547)
                     /locus_tag="PKH81_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97414.1"
                     /translation="MILTPASRHEIPRGASFFQTGFWAAFKESRRQFVRCFLLQSSSR
                     ITPLVVCVRQGVGGIPYAYVPKGPGTTLDDEDRGLFLEELSLLLQEHLGDVACVRYDT
                     EFPTPYTQGEYWSDNGHWKGPPRTEIRELRMNYGTRTRMLRKAPYDHFSPDTVLVDLR
                     GSESDVLARMRQTTRHSIRKAC"
     gene            complement(571..1092)
                     /locus_tag="PKH81_04930"
     CDS             complement(571..1092)
                     /locus_tag="PKH81_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNQ97415.1"
                     /translation="MFPLAQLLVALIIFVARVVDVSLATFRHAMIVRGRKAYALVVAF
                     VESLIWIYAVSRVLSDLTEPVTALAFAAGFACGTWVGITIEDMLKIGEQVVRIFSAQG
                     DSVSAALRDVGYRVTLFEGSGRDGPVQLLFTQVKRRDVRKVNDLARSIDPSCYLVIDD
                     IRFASTGVHQIRT"
     gene            complement(1174..1575)
                     /locus_tag="PKH81_04935"
     CDS             complement(1174..1575)
                     /locus_tag="PKH81_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNQ97416.1"
                     /translation="MKFDLIKELNADHSALVEMLKRIGAKGIIDDDTVKQLQEFRAAL
                     IAHLQKEDREFYPVMNKAAETNPALAETLKVMGMEMEQLADKALKMIDGWLAGLGSTS
                     FAADFQIFCSLLAARIKKEENSLYSRYLKLA"
     gene            1630..2058
                     /locus_tag="PKH81_04940"
     CDS             1630..2058
                     /locus_tag="PKH81_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNQ97417.1"
                     /translation="MLNNSVIHFSDAALIAVHALATLARNKDNLVQTQALAEELGSSP
                     HHVAKVMQRLVHSRLVSSVKGPSGGFTLAIEPSHISFKTAIEAVDGPLEANFCPFRSE
                     VCSPDSCIFGKELADHAKALVKHLSERTIEDIEILKKRKR"
     gene            2119..>2318
                     /locus_tag="PKH81_04945"
     CDS             2119..>2318
                     /locus_tag="PKH81_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ97418.1"
                     /translation="MAQRTIIQIDEDLCDGCGLCANGCPEGALRIIDGKARMVGDSLC
                     DGLGACIGDCPRGAITTIVREAT"
BASE COUNT          676 a          568 c          518 g          556 t
ORIGIN      
        1 gacatgcttt tctgatagag tgtctcgtcg tttgtcgcat acgtgcaagt acatcagact
       61 cgcttcccct tagatctacc aaaacagtat ccggagaaaa atgatcatac ggagcctttc
      121 ggagcatccg agtacgagtt ccataattca ttcgtaactc gcgtatctcg gttcgcggag
      181 gccctttcca gtgtccatta tcgctccagt attcgccctg agtatagggc gtcgggaatt
      241 cggtatcata gcgaacacag gcaacatcac ccaagtgctc ttgcagtaac agggacaact
      301 cttccaggaa caaaccacga tcctcatcat ccagtgttgt ccctggaccc tttggtacat
      361 aggcataggg aatacctcct acgccttgtc gtacacacac cacaagaggg gttatcctgg
      421 aggaagattg aagtaaaaaa catcgaacaa actgtctgcg agattccttg aacgccgccc
      481 aaaaacctgt ttgaaaaaat gaagcacccc gcggtatttc gtgccgcgag gcaggggtga
      541 gtatcattga atattccttg taatgttatg ttatgttctg atctgatgca cgccagtaga
      601 tgcgaatctg atatcgtcta ttactagata acaagacggg tctatcgatc gagccagatc
      661 gttaaccttg cgaacatcgc gccgcttaac ctgagtaaac aacaattgaa caggaccgtc
      721 tcgaccagat ccctcaaaaa gggtaacccg gtaaccgaca tcccgcaggg ctgccgatac
      781 actgtcgccc tgcgctgaaa aaatccgcac aacctgctcg cctattttaa gcatgtcctc
      841 aatagtgata ccaacccagg taccacaggc aaaacctgca gcaaaagcaa gagcagtaac
      901 cggttcggta aggtctgaaa gaacgcgaga aaccgcgtat atccagatta gggattcaac
      961 aaaagctacc acaagtgcgt acgcttttct gcctcgaacg atcatcgcgt gacggaaagt
     1021 cgcaagagaa acatcaacca cgcgagcgac aaaaataatc agcgccacaa ggagctgtgc
     1081 aaggggaaac acggcgttac ctcttaaacg tattttgcct aagcaatacc tcaaagatac
     1141 aagctaaacg gcaaaggggc aagcagactt cgcttaggca agcttaagat agcgcgaata
     1201 aagcgagttc tcttctttct tgatgcgagc agccaataag gaacaaaaaa tctgaaagtc
     1261 agcagcaaag gacgttgaac caagccctgc aagccaaccg tcaatcattt tgagcgcctt
     1321 atctgccagt tgctccattt ccatacccat taccttgaga gtctccgcaa gagcaggatt
     1381 tgtctctgca gccttattca ttaccggata gaattcccga tcctcctttt gaagatgtgc
     1441 gattaaggcg gcccggaatt cctgtaactg tttaacagta tcgtcgtcga taataccttt
     1501 agcgccaata cgcttaagca tttcgaccaa tgcactgtga tcggcattaa gttccttaat
     1561 caaatcaaat ttcatagatt cctcctggta gtattgacag tatatatatt ctggtattaa
     1621 attaccagta tgcttaataa cagcgtcata catttttccg atgccgctct catcgccgta
     1681 catgcgctgg cgaccctcgc tcgaaacaag gacaacctag tgcaaaccca ggctcttgcg
     1741 gaagaattgg gatcgtcacc tcaccatgtt gccaaggtaa tgcagagact ggttcactct
     1801 cgcctggtta gttctgtaaa gggaccttcg ggaggattca ccctggcgat agaaccttcg
     1861 cacataagct tcaagaccgc aatcgaagca gtagacggac cactagaagc aaatttttgc
     1921 ccatttcgaa gcgaagtctg ctcgcccgac tcatgtattt ttggtaaaga actcgctgat
     1981 catgcaaaag cacttgtaaa gcatctttct gagcgcacaa tcgaagatat tgaaatacta
     2041 aaaaaaagga aaagatagtt tttttctcat aaagcggtat tacggtacca catagctata
     2101 ttgataagga gaatacaaat ggcacagaga acaataattc aaattgacga ggatctatgt
     2161 gatggttgtg gtttatgcgc aaacggatgt ccggaaggag ccttgcggat aatcgatggc
     2221 aaggcacgaa tggtaggaga cagtctttgc gacgggcttg gtgcttgcat tggcgattgt
     2281 cctcgtggtg cgattaccac catcgtacgg gaagcaac
//
LOCUS       DAOQXQ010000252         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_52456_length_2310_cov_3.059867, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000252 DAOQXQ010000000
VERSION     DAOQXQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52456_length_2310_cov_3.059867"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PKH81_04950"
     CDS             complement(<1..170)
                     /locus_tag="PKH81_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNQ97419.1"
                     /translation="MAKKKGPTQATSGWLNTYADMITLMLCFFVMLYDPSEADVVQMQ
                     QMTASIANDATGG"
     gene            complement(205..984)
                     /locus_tag="PKH81_04955"
     CDS             complement(205..984)
                     /locus_tag="PKH81_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HNQ97420.1"
                     /translation="MDIASFIGLFGGLGVVMFGALLGGSLGSLIHLPSLFITGGGSYM
                     CLFLTFPLSYVVGIFSVLGRCFKTFDYGEKTLVQKLVSFSEKARREGILALEEEIQDL
                     DDEFMRNGLRFVVDGTDGEVIRALMENELNQMESRHNSWITLVNAWATLAPGFGMLGT
                     VIGLIGMLSNLADKSSLGPNMATALITTLYGSMMQNWLFVPMSGKLGYQNSMEAKSRE
                     MVIEGVLSIQAGDNPRILAQKMLTYLSPKDRKVIESDVLKD"
     gene            complement(991..1188)
                     /locus_tag="PKH81_04960"
     CDS             complement(991..1188)
                     /locus_tag="PKH81_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNQ97421.1"
                     /translation="MIAVTQLNGKQYWINPHQIECIEANPDVTLVMLSGRHVVVREKP
                     EDIISSIVEYRRRIGAFKNEE"
     gene            complement(1281..>2310)
                     /locus_tag="PKH81_04965"
     CDS             complement(1281..>2310)
                     /locus_tag="PKH81_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNQ97422.1"
                     /translation="NQGTFVNPGNGMRVQGWMAQDLEGQLVLNTSTQAGDLTIPVGSK
                     IEARATTNVNYACNLDKRLPEIPEGATEADVSKATWATEFQVYDSFGQQHTLNVSFTR
                     VPGTNNQWQATVNVDPTEAEAAQTRVGLGTTDGTGNTFIVNFDNNGMLAGATDTAGNA
                     SATEGNVALDVSFNVAGANPGEDGEPYRQTFSINLGEIGSVRNTITQFSEKSSTKAYE
                     QDGYTMGYLENFKIDQSGVITGVYSNGANRMIGQIAMATFANQGGLEKAGENTYVQSN
                     NSGLANIGVSGVAGKGKLIAGALEMSNVDLTEQFTDMIITQRGFQASSKTIQTSDTML
                     DTVLNLKR"
BASE COUNT          575 a          586 c          526 g          623 t
ORIGIN      
        1 cctccggtgg catcgttggc aatagacgcg gtcatttgtt gcatctgaac cacatctgct
       61 tctgaaggat catagagcat aacaaagaag cagagcatga gggtgatcat atcggcatag
      121 gtattaagcc agcctgaggt tgcctgtgtt ggtccctttt tttttgccac ttgcccgtaa
      181 ccctccctta cctgctctct tgcgttagtc ttttagaacg tccgattcaa taaccttgcg
      241 gtccttggga gaaagatagg taagcatttt ttgcgccaag atgcggggat tgtctccagc
      301 ctggattgaa agcacgcctt cgataaccat ttcccggctt tttgcttcca tactgttttg
      361 atagcccagt tttcctgaca ttggcacaaa cagccagttc tgcatcatag atccatataa
      421 ggtggtgatt agagcggttg ccatgtttgg accgagagag ctcttgtctg cgaggtttga
      481 aagcataccg ataagaccaa taacggttcc aagcattcca aaaccagggg caagggtcgc
      541 ccaggcgttt accagggtta tccaggaatt gtgtcgactt tccatttgat tgagctcatt
      601 ttccattaaa gcgcgaataa cctctccgtc tgtaccgtca acaacaaacc tcagtccatt
      661 tcgcataaac tcatcatcaa ggtcctgtat ttcttcttcc agggccagga taccctcgcg
      721 tcgtgccttt tcggaaaacg aaacaagctt ttgaacaagt gttttttctc cgtaatcgaa
      781 tgttttaaaa caacggccca atacactaaa aatcccgact acataggata gcgggaacgt
      841 gagaaaaaga cacatgtatg atccaccgcc ggtgataaac agggacggaa ggtgaataag
      901 gcttccaagg gaaccgccga gtagggctcc aaacataacg acgcccaaac cgccgaacaa
      961 accaatgaat gacgcgatat ccatagtttc ctactcctcg ttcttgaacg cgccgatacg
     1021 gcgtcgatac tcgacgatac ttgaaattat atcttcgggt ttctcccgta ctaccacatg
     1081 acggcccgag agcatgacga gcgtcacatc gggatttgcc tcgatgcatt ctatttgatg
     1141 ggggttaatc caatactgtt ttccgtttag ttgcgttacc gctatcatct gttacctttg
     1201 gaaataacca tacctgtccg ccaaagcgga aggcgcggga tagtaacctc aagggaaacc
     1261 atcccgctta ctatatctta ttaccgcttc agattcaaca cggtgtcaag catcgtgtct
     1321 gaagtctgaa ttgtcttact gctggcctga aaaccgcgct gagtgataat catgtctgta
     1381 aattgctcgg tcaggtctac gttactcatt tcaagtgcac ccgcgatgag cttacccttc
     1441 cctgccacac ccgagacacc gatattggca agccctgagt tattggactg gacatacgtg
     1501 ttctctccag ccttttcaag accaccctga ttggcaaagg ttgccatggc aatttgaccg
     1561 atcatacggt ttgcaccgtt tgaatagaca ccagtgatca caccggactg atcaatcttg
     1621 aagttctcga gatatcccat agtgtatcca tcttgttcat acgctttggt ggaactcttt
     1681 tcgctaaatt gagtgatggt attgcgtaca ctgccaattt cgccaaggtt gatcgagaat
     1741 gtctggcgat acggctctcc gtcctctcca gggttagcac cggctacgtt gaaagaaaca
     1801 tcaagggcga catttccctc tgtagcagaa gcgtttccgg cggtatcagt agctccggca
     1861 agcataccgt tattgtcaaa attaacgata aacgtattgc ctgttccatc ggtagtacca
     1921 agtccgacac gggtttgagc cgcctcagct tcggtaggat ctacgtttac cgtagcttgc
     1981 cactgattgt tagttcccgg aacccgggta aacgaaacgt taagggtatg ttgctggcca
     2041 aagctgtcgt atacctggaa ctcggttgcc catgttgcct tgctcacatc agcctcggta
     2101 gctccttccg gaatttcagg aaggcgttta tcaaggttac aggcgtaatt cacattcgta
     2161 gtagcgcggg cttcaatctt ggaacctacc ggaatggtta agtcccctgc ctgggtagaa
     2221 gtattgagta caagttgtcc ttcaagatcc tgagccatcc aaccctgaac gcgcatacca
     2281 ttaccgggat ttacaaaggt tccctgattg
//
LOCUS       DAOQXQ010000253         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_52973_length_2295_cov_3.570089, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000253 DAOQXQ010000000
VERSION     DAOQXQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52973_length_2295_cov_3.570089"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKH81_04970"
     CDS             <1..349
                     /locus_tag="PKH81_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97423.1"
                     /translation="APTGTLLPIEPGHGSSARLILQGNNTLSGTTGSYTFEGTWKAGK
                     PNAAGEFPFSAVITKVQGQEPANAIASRFEADLIRLLKKTSFVRLGRGNIHLMDKNKA
                     PTLQYIHRSADAP"
     gene            complement(353..1732)
                     /locus_tag="PKH81_04975"
     CDS             complement(353..1732)
                     /locus_tag="PKH81_04975"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNQ97424.1"
                     /translation="MSDKQFDFSIASLGPCKVKSPIQLSSVEGDFIANYVTDDEFIRY
                     RLDTTTDDVVGPCARSELLEKAGPRENIFFNPSHVHAAIVTCGGLCPGLNDVVRSVVR
                     CLWNRYGVKRISGIRFGFKGLLSEYGFETLPLTPAIVDDCHKNGGSILGTSRGGGDRV
                     IEIADAIENMNINMIFIIGGDGTQRGSLEVAEEIEKRGLKIAVIGIPKTVDNDLSFIQ
                     KSFGFDTAVVKAAESVAAAHMEAHSQINGIGLVKLMGRESGFIATHTAIASHEANFVL
                     IPEVPFELEGPNGFLHHLEQRLRKRHHAVIVVAEGAGQDLLKAEEGTDDSGNKKLADI
                     GVFLRDKMATYFKSIGMHINLKYIDPSYQIRSAPAAPIDSIYCERLGNNAVHAAMAGK
                     TKLIIGLVHNKFVHLPISVVTSKRNYVDPEGSLWRDCLDATSQPILMVNDRDAFLKNS
                     LNRNRGKNR"
     gene            1853..2074
                     /locus_tag="PKH81_04980"
     CDS             1853..2074
                     /locus_tag="PKH81_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNQ97425.1"
                     /translation="MEEMVKKALEDIRPQLQADGGDIEFISVGADGKVLVRLTGACGS
                     CPMATMTLKMGVEAHLKKTVPGVTEVVQA"
     gene            2080..>2295
                     /locus_tag="PKH81_04985"
     CDS             2080..>2295
                     /locus_tag="PKH81_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNQ97426.1"
                     /translation="MNRNAFLPVSRDDLASRGWDDCDFIIVSGDAYVDHPSFAAAILG
                     RLLESQSFSVGIIAQPEWQGPQSEKDFT"
BASE COUNT          618 a          593 c          491 g          593 t
ORIGIN      
        1 tgccccaact ggaaccttat tgccaattga accaggtcat ggctcatccg cacgattaat
       61 cctgcaggga aacaatactc tttcggggac aactggttca tatacctttg aaggaacatg
      121 gaaggcagga aaaccaaacg ccgcaggaga attccccttt tccgcagtaa taacaaaagt
      181 tcagggacaa gaacctgcta acgctatcgc atcccgcttt gaagcagacc ttattcgtct
      241 tctgaaaaaa acctcgtttg ttcgtcttgg ccgaggcaac atacacctta tggataaaaa
      301 caaagcgccg acccttcagt atattcaccg atcggccgac gctccctaat ccttacctgt
      361 tttttccccg gtttctattc aagctattct ttagaaacgc atccctgtcg ttcaccatta
      421 aaatcggctg gcttgtcgca tcaaggcaat cgcgccacag agatccctca ggatcaacat
      481 agttgcgttt gctcgttact acactgatgg gcaaatgcac aaacttatta tgcacaagtc
      541 caataatcaa ttttgtttta ccagccattg ctgcgtgtac cgcgttgttc cccaatcgct
      601 cgcagtaaat cgagtcaatg ggagctgcag gggcagatcg aatttgatag ctcggatcaa
      661 tgtatttaag gttaatgtgc atcccgattg acttaaaata agttgccatc ttgtcgcgca
      721 agaatacacc aatatcggca agcttcttgt tgccggaatc gtcggttcct tcctcggctt
      781 ttagtaaatc ctgccctgct ccttcggcaa caacaattac tgcatgatgg cgtttgcgca
      841 gtcgctgttc aagatgatga agaaagccat tgggtccttc aagctcaaac ggaacctcgg
      901 gaataagaac aaagtttgcc tcgtgacttg ctattgccgt atgcgtggct ataaagccgg
      961 attctcgccc catcagttta acaagcccta tgccattgat ttgtgaatgc gcttccatat
     1021 gagcagccgc gaccgattcg gctgccttta ccaccgcggt atcgaatcca aaggattttt
     1081 gaataaaaga aagatcgtta tcaactgttt taggaatacc aataacggct atcttaaggc
     1141 ctcgcttttc aatctcttcc gcaacttcaa gggagcctct ctgagtccca tcgcctccaa
     1201 taataaatat catgtttatg ttcatattct ctattgcgtc cgcgatttca attacccggt
     1261 ctccaccacc gcggctcgtt cccaggattg aacctccatt cttatggcaa tcgtccacaa
     1321 tagccggcgt aagcggcaag gtctcaaagc catattcact caataatccc ttaaacccaa
     1381 agcgtatccc gctgatacgt ttaaccccat accggttcca caaacatctg acaactgagc
     1441 gtacaacatc gttaagccca ggacacagac cgccacaggt tacaatagcc gcatgcacat
     1501 ggctcggatt aaaaaagata ttctctctcg gaccggcttt ttccaaaagc tcggatcgag
     1561 cacaggggcc aacaacatcg tccgttgttg tatcgagtcg ataacggata aactcgtcgt
     1621 ccgtaacgta attcgcgata aaatcgcctt caacactgga caactgaatc ggcgacttta
     1681 ccttacaggg tccaagagaa gcgatactaa aatcaaattg tttatcagac atgtctatct
     1741 cctgtggaaa gtatattgaa atagaaggcg ggaaacaata gagaacttga cccttatagc
     1801 ctcgcgctgg tatcttttgc gaaacccaca tggtttttta ggagtattta ttatggaaga
     1861 aatggtaaag aaagcccttg aggatatacg gccccagctt caagcagatg gcggggatat
     1921 tgagtttatc tctgttggcg cagacggaaa ggtgcttgtt aggctcacag gagcctgtgg
     1981 ctcatgcccc atggccacaa tgacccttaa aatgggtgtt gaagctcacc ttaaaaagac
     2041 cgttcccggc gttacagagg ttgtgcaagc gtaaacttga tgaacagaaa cgcctttctt
     2101 cccgtctccc gggacgatct agcttcccgg ggctgggatg actgcgattt tatcattgta
     2161 tctggagacg catacgtcga ccatccttca ttcgcggcgg ccatcctggg ccgtctcttg
     2221 gaaagccaga gcttctcggt aggaattata gctcaaccag agtggcaagg ccctcaatcc
     2281 gaaaaagact tcacg
//
LOCUS       DAOQXQ010000254         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53078_length_2292_cov_2.706750, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000254 DAOQXQ010000000
VERSION     DAOQXQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53078_length_2292_cov_2.706750"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PKH81_04990"
     CDS             <1..167
                     /locus_tag="PKH81_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97427.1"
                     /translation="YPGVGRLMYEAVIQEDINLVLANIMFISALVLVGMVLADITLAL
                     VDPRIRYGKE"
     gene            206..1282
                     /locus_tag="PKH81_04995"
     CDS             206..1282
                     /locus_tag="PKH81_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97428.1"
                     /translation="MISAVFLLTVYLAMAFAEFIAPYSPGTSFPQMTYHPPNVRVSSH
                     GVTAQEARTVNPVSWSYVRVKELHHPLSLFVKGEPYRLLGFIETDRHLFGVADSAYPV
                     FLLGADNLGRDLFSRIVYGSRISLTIGFIATAISLFLAILFGGLSGFFGGMTDWSIMR
                     FSEFFMLIPGLYLILFLRSLLSSTMDSGQSYMVITIILSLVGWPGSARTVRGLVHSIK
                     REEFVLNAQLEGIPSVVIIFRHIIPQMASLLIVSIALSVPGFIMSETTLSYLGLGIVD
                     PAVSWGSLIRRDISTLSNLSNFPWLLSPVWMLLGVTLSFNFIGDALRDFYDPYHAVFK
                     RFSLRTMLSRITGRFMPKVEGGAQ"
     gene            1279..>2292
                     /locus_tag="PKH81_05000"
     CDS             1279..>2292
                     /locus_tag="PKH81_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97429.1"
                     /translation="MSEHSFLRVENLSVTFPPKDARLGGPVFAVRGVSWTLEKGEILG
                     IVGESGSGKSVSTSAIPSLLPAHAEVTGDIFLEGKQLVGLPTEDIRSFRGRRIGMIFQ
                     EPGRSYDPLQNIGSVFHETLANARPGISRLESEEEAIRLLTEVGLPQPRERLGNYPHQ
                     FSGGQLQRIGIALALAQGCDLLIADEPTTALDVTIQAQIVALLKRIRDDRAISILFIS
                     HNIDLVATISDRILVMYGGLVMEEGPTRQVLASPAHPYTAALLAAAPRFDSHYSTSPL
                     LSIPGKVPDPSRPLPGCPFAPRCSRVMSGCRTELPPLRWGPQGADESLARSWRCVFDR
                     EEVR"
BASE COUNT          469 a          545 c          614 g          664 t
ORIGIN      
        1 cgtatccggg tgtaggccgg cttatgtacg aagcggttat tcaggaggac atcaatttgg
       61 tattagccaa tattatgttt atctcggcct tggtccttgt tggaatggtg ttggcagata
      121 taacgttggc cttggttgat cctcgaataa ggtacggtaa agaatgaaag ggttttacgc
      181 atttttgcgc acgcggccgc ttgcaatgat atctgctgtg tttctactaa cggtgtatct
      241 ggcaatggcg tttgccgagt ttatcgcccc atatagtccg ggaacctcgt tcccgcagat
      301 gacctatcat ccgcctaatg tgcgcgtatc ctcgcacggg gttaccgcgc aagaagcgcg
      361 aacggtcaat ccggtaagct ggagctacgt gcgggtaaag gagcttcatc atcccctgtc
      421 tttgtttgtt aaaggagagc cgtatcgctt acttgggttt atagaaacag accggcattt
      481 atttggtgta gctgattccg cctatccggt atttcttctg ggagcggaca acctggggcg
      541 ggatttattt tcgcgcattg tctacggtag cagaatctcg ctaaccattg gttttatcgc
      601 aacggcaatt tctttattcc ttgctatttt atttggcgga ctttcgggct tttttggcgg
      661 catgacggac tggtccatca tgcgcttttc ggaatttttt atgcttattc ccggcctcta
      721 tctgattctt tttttgcgct ccctcttgtc ttctaccatg gatagcggac agtcatatat
      781 ggtaatcacg ataattcttt ctcttgtggg ctggcccggt tcggcgcgta ctgttcgcgg
      841 gttggtgcat tcaatcaagc gggaggagtt tgtactgaat gcgcagctgg aaggaatccc
      901 ttcggtagta attattttcc gccacattat tccacaaatg gccagcctct tgattgtcag
      961 tatcgcctta agcgttcccg gttttattat gagcgaaaca actctgtcgt atcttggcct
     1021 tggtattgtt gaccccgcgg taagctgggg ttcgcttatc aggcgggata tttcaacgct
     1081 gagtaacctc agtaacttcc cctggctctt aagcccggta tggatgctgc tgggggttac
     1141 cctttctttt aattttattg gggacgccct gcgagatttt tatgacccct accatgcggt
     1201 gtttaaacga ttttcgctgc gtacaatgct ttcaaggatt actggtcggt ttatgccaaa
     1261 agtagaaggg ggtgcgcaat gagcgaacat tctttcttac gggtagaaaa cctttcggta
     1321 acctttcctc ccaaagatgc acgattaggg ggaccggtgt ttgcggttag gggcgtgtcc
     1381 tggacgctgg agaagggaga aatacttggc atcgtcgggg aaagcggttc cggaaagagt
     1441 gtgtcaacct ccgcgatccc atctctttta ccagcccatg cggaagttac aggagacatc
     1501 tttcttgagg gaaagcagct tgttggattg ccgacagagg atatccgctc ctttcgtgga
     1561 aggcgcatcg ggatgatttt tcaggagccg gggcgctctt atgacccgct tcaaaatata
     1621 ggaagtgtgt ttcacgaaac ccttgcaaac gctcgccctg gtatatcgcg gctagaatca
     1681 gaggaagaag cgattcgtct tttaacggag gttggtttgc cccagccgcg ggagagactg
     1741 ggtaattatc cgcatcagtt ttccggcggt cagcttcaaa gaataggtat cgctcttgca
     1801 cttgcgcagg gctgcgattt acttattgca gatgagccaa caaccgcgct tgatgtaacg
     1861 atacaggcac aaattgtagc cctgcttaag cgcattcgcg acgatcgggc tatatcgatt
     1921 ttatttatca gtcataatat tgatctggtc gccacaatct ctgaccgaat attggttatg
     1981 tatggaggcc ttgtaatgga agaagggcct accaggcagg tgcttgcatc acccgcgcat
     2041 ccgtatactg ccgcgcttct tgctgccgcg ccccgctttg acagtcacta tagtacttct
     2101 ccccttcttt ccattccggg caaggtgccc gatccttcgc gcccgcttcc gggctgcccc
     2161 tttgcgcccc ggtgttcgcg cgttatgagc ggatgtcgga cggagctgcc tccgcttcga
     2221 tgggggccac aaggagcaga tgaatcttta gcccgaagct ggcgatgtgt gtttgaccgc
     2281 gaggaggtaa ga
//
LOCUS       DAOQXQ010000255         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53085_length_2292_cov_2.581582, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000255 DAOQXQ010000000
VERSION     DAOQXQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53085_length_2292_cov_2.581582"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKH81_05005"
     CDS             complement(<1..675)
                     /locus_tag="PKH81_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNQ97430.1"
                     /translation="MMSSNTVRLSDIIATSFHEIHKQIKSGGAQEVWCKGGRGSTKSS
                     FLSIETLLTLQKDPKAHAFISRRYDNELRDSVYGQMQWAAHKLNLDHIWRFMVSPMQA
                     VNQVTGQKILFRGVDNPIKAKSINLGFGYIKVFWAEEVDQYGSMEELRTIMQSLFRGE
                     GSGQMAFFSFNPPKSARAWVNAETKIKKVGRVVHSSDYRTVPTDWLGERFLTDAEHLR
                     KTNEAAY"
     gene            complement(662..994)
                     /locus_tag="PKH81_05010"
     CDS             complement(662..994)
                     /locus_tag="PKH81_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97431.1"
                     /translation="MSSCKGYTEKKVLAAIKGSGGIITTISNRLGSDWHTAKKLTQQW
                     QSTRDAYKDERETILDMSESAVLKSIKEGNTQDAKWILSTLGKNRGFSEKVDIELSGS
                     VTIIDDVK"
     gene            complement(987..1772)
                     /locus_tag="PKH81_05015"
     CDS             complement(987..1772)
                     /locus_tag="PKH81_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97432.1"
                     /translation="MDINVKDIIIKPINSTTANAFVKKHHYSGKVTSNSQLHFGAFYK
                     DVLHGVMSFGPSLDKSKIIGLVKDTKWNDFLELNRMAFDDYLPKNSESRSISVAMKLI
                     KKHYPNIEWVVSFADGTQCGDGTIYRASGFVLTMIKENTDCMKTESGVVIHTMTVKSS
                     KSLMSKYGDWKTAMKQTYGEYKPLKGFQLRYIYFLNKEARNRLTVPILPFSAIDEMGA
                     GMYLGKKKTSGGSVTVAQTAIQPERGGSIPTSPHNTDRGLTNE"
     gene            complement(1762..>2292)
                     /locus_tag="PKH81_05020"
     CDS             complement(1762..>2292)
                     /locus_tag="PKH81_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNQ97433.1"
                     /translation="NRDKLMASILEKGFIAPIFVWDDAGEYRLLDGHQRLKTLLWMRE
                     KGWDIPMLPVDIIEADDEQDAKKKLLAITSQYGEFTTDGYAEFINDIEVEDCVRLVDG
                     EWSQDGIDCATDFPELKDGDKEPFQQMTFTLADEQAELVKEAIRKAQEEGCESEANQN
                     RNGNALAMICEVFIGH"
BASE COUNT          492 a          596 c          482 g          722 t
ORIGIN      
        1 gtaggcggcc tcgtttgtct tccgcaggtg ttccgcgtcg gtcaggaagc gttccccaag
       61 ccagtcggtc ggtacggttc tgtagtctga ggaatgtacc acgcgcccga ccttcttgat
      121 cttcgtctcg gcgttcaccc atgcccgagc ggacttcggc gggttgaatg aaaagaacgc
      181 catttgcccg gagccttcgc ctcggaaaag tgactgcatg atcgtccgaa gctcttccat
      241 gcttccgtac tggtcaacct cttccgccca gaatactttg atatatccga aaccgagatt
      301 gattgatttt gccttgatgg gattatcaac gcccctgaaa aggatctttt gccctgtcac
      361 ctggttaact gcctgcattg gcgagaccat gaagcgccag atatggtcaa gattcagttt
      421 gtgcgccgcc cattgcatct ggccgtaaac agaatcacgg agttcattgt cataccttct
      481 ggaaataaac gcatgagcct ttgggtcttt ctgcaaagtt aaaagcgttt cgattgacag
      541 gaagcttgac tttgttgatc cgcgcccgcc cttgcaccag acctcctgcg cgccacctga
      601 tttgatttgt ttgtggatct catgaaaaga agttgcgatt atgtcggata gccttaccgt
      661 gttacttgac atcatctatg attgttaccg atccggaaag ctctatatct actttttcgc
      721 tgaaacctcg gttcttccca agcgttgaaa ggatccactt tgcgtcctgt gtgttccctt
      781 ccttgattga cttcagcact gccgattcgc tcatgtcaag gatcgtctcg cgctcatctt
      841 tgtacgcgtc gcgcgtagac tgccattgtt gcgtgagctt cttggctgtg tgccaatctg
      901 agccgagacg atttgatata gtggtgatga ttccgcccga tcctttaatg gcggcgagca
      961 cctttttctc ggtgtatccc ttacagctac tcattagtta atcctcgatc tgtattatgc
     1021 ggcgaggtcg gaattgaacc gcctctttcc ggctggattg ccgtctgtgc taccgtaaca
     1081 cttccgccgc ttgttttctt tttaccaaga tacattcctg cgcccatttc gtcaatggca
     1141 gaaaatggaa gtattggaac cgttaaacga ttgcgcgcct ccttgtttag aaagtatatg
     1201 tatcgaagct gaaaaccttt caacggttta tattctccgt atgtttgttt cattgcggtt
     1261 ttccagtctc cgtatttact cattaacgac ttactgcttt ttacggtcat tgtatgtata
     1321 acaactcctg attctgtttt catacagtct gtgttttctt ttatcattgt caaaacaaac
     1381 ccgcttgccc tgtatatcgt gccgtctccg cattgtgttc cgtcggcgaa tgatacaacc
     1441 cactcgatat tcggatagtg cttttttatt agtttcattg caactgatat tgaccttgac
     1501 tcgctgtttt tcggaaggta atcgtcaaac gccattctgt taagctcaag aaaatcgttc
     1561 cattttgtat cttttacgag tccaattatt ttgcttttat ccagcgacgg tccgaaactc
     1621 attacaccat gcaacacgtc tttataaaac gcgccaaaat gaagttggct attacttgtc
     1681 accttcccgc tataatgatg ctttttaaca aaagcgttcg cggttgttga gtttatcggc
     1741 tttattatta tgtcttttac gttaatgtcc aatgaatacc tcgcatatca tagcaagcgc
     1801 gtttccgttc ctgttctgat tcgcctcgct ctcgcaccct tcttcctgcg ccttccttat
     1861 tgcctctttt accagttcgg cctgttcatc cgcaagggta aatgtcatct gctgaaatgg
     1921 ttctttgtcg ccatccttca actcaggaaa atcagtcgcg caatcaatgc cgtcctgcga
     1981 ccattcaccg tcaacaagcc tgacacaatc ctcaacctcg atgtcgttta taaactcagc
     2041 atatccgtcc gttgtaaact cgccgtattg agaagtaata gccaataact tcttctttgc
     2101 gtcctgttca tcgtcggctt cgattatatc caccgggagc attgggatgt cccagccttt
     2161 ctcgcgcatc caaagaagcg tcttgagtcg ttgatgtccg tcaagtagcc ggtattcccc
     2221 tgcgtcgtcc caaacaaaaa taggagcgat aaaccctttt tcaagtatcg acgccatgag
     2281 cttgtcacgg tt
//
LOCUS       DAOQXQ010000256         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53151_length_2290_cov_3.039821, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000256 DAOQXQ010000000
VERSION     DAOQXQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53151_length_2290_cov_3.039821"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="PKH81_05025"
     CDS             complement(<1..2021)
                     /locus_tag="PKH81_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ97434.1"
                     /translation="MKNERIYIVEDERIIAIDLKRRLERMGYQVCGSASDGETALAGI
                     RTEKPDLILMDVVIQGPMDGIEVARIIYTELHIPVIFLSAYTDGLTLDRARDAHPLGY
                     ILKPFKERELANLLEMALYKSSADTQIREKEQLFSAILNSTTDAILVLGRSNEVLFIN
                     PEAEQILEIKDTEARAKRLQDLLTLMDMETAEPFSTPDFSDRLKILKAHNLRLTNHRG
                     NSFVVEMTVNHEVRVGQSHENYIVSFKDISRLHEMTDAIRYQTSHDILTGLLNRNELA
                     LRMNTTLAKAPISGSSAHALFIDVDHFRVVNDSCGTQAGDVLLKEIADRIRTHVTPKN
                     YAARTGGDDFVLVYIQDGPSQKDVSVIARDLLEAARAHPFKWNRKEYPITLSIAIVPL
                     DSSFKSEHDVMIAGAQTVLATHESGGGRFCFYERTDGGRYGSLPISEWISRIHSALQN
                     DGFRLYYQPIEPLSGTGKRHKLEVLIRMVDESGAIIQPGDFIPIAERYNIMPAIDRWV
                     IKNSFRQYAELSRAGDPLADRVFSVNLSGASLVDGEIIEYIIDKAREYRVPPAQFCIE
                     ITESNAILNLTSASRIIHILHEQGFTFALDDFGSGFSSFSYLKNLPVDYLKIDGSFIR
                     NMDKDHVDYTMVQAITSMCHVLSLEVVGEFAENGAIIKMLHEIGVDYAQG"
     gene            complement(2018..>2290)
                     /locus_tag="PKH81_05030"
     CDS             complement(2018..>2290)
                     /locus_tag="PKH81_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNQ97435.1"
                     /translation="NQAAKQALSGKGSVIYLEADFDVVFDRIMESARRDGRLPPFLQG
                     DNPKENFRVIFTRRAECYVTMCSLRVQTGLLTQSDIVEIIISKLFA"
BASE COUNT          528 a          577 c          612 g          573 t
ORIGIN      
        1 ccctgcgcgt agtctacccc aatctcgtgg agcatcttga taatcgcccc gttttccgcg
       61 aactctccca ccacctcaag cgacaataca tgacacatac tggtaatagc ctgaaccatg
      121 gtgtaatcga cgtgatcttt atccatgttg cggataaagc ttccgtcaat ttttagataa
      181 tctaccggca agttcttcag gtagctaaag gacgaaaagc ctgacccaaa atcgtcaagg
      241 gcaaaggtga acccttgttc gtggagtata tgaataatcc gcgatgccga ggtcaggttg
      301 agtatggcat tggattcagt aatctctatg cagaattggg ccgggggcac ccggtattcc
      361 cgcgccttat cgataatata ttcgatgatt tcgccatcca ccagggatgc gccggaaagg
      421 ttaaccgaga atacccggtc ggccagaggg tctcccgcac gggaaagctc tgcgtattgg
      481 cgaaaactgt tcttgattac ccagcggtca atggcgggca taatgttata gcgttcggct
      541 atgggaataa aatctcccgg ttgtataatc gctcctgact catctaccat gcgtataagc
      601 acttccagct tgtgtctctt cccggttccc gaaagaggct caattggctg gtagtacagt
      661 ctgaacccgt cattttgcaa tgctgagtga atgcggctaa tccattcgct tatcggtagc
      721 gagccgtagc ggccgccatc cgttcgctca taaaaacaaa atcgcccgcc gccactttcg
      781 tgcgtggcca acaccgtttg cgctccggct atcattacat catgttcgct tttaaacgat
      841 gaatccaatg gcacaatggc gatgctcagg gtaatcgggt attccttgcg gttccacttg
      901 aacgggtggg ctcgggctgc ctctaaaagg tcgcgtgcga taaccgacac atccttttgg
      961 cttggcccgt cctgaatgta caccagaaca aagtcgtcgc cgccggtgcg ggctgcataa
     1021 ttcttggggg taacgtgagt gcggatacga tcggcaattt ctttaagcag aacgtctcct
     1081 gcctgggttc cgcaggaatc gtttaccacg cggaagtgat ccacatcgat aaacagggcg
     1141 tgggcgctag agccggaaat gggagccttt gccaaggtcg tattcattcg cagggccagt
     1201 tcattgcggt taagaaggcc cgtcaggatg tcgtggctgg tttggtagcg gatggcatct
     1261 gtcatctcat gcagacgcga aatatcctta aaggaaacga tgtagttttc gtgcgactgg
     1321 ccaacgcgaa cctcgtggtt aaccgtcatc tccaccacaa aggaattgcc ccgatgattg
     1381 gtaaggcgaa ggttgtgggc cttaagaatc ttgagccggt cggaaaagtc gggggtgcta
     1441 aacggctcgg cagtttccat atccatcagc gtaagtaagt cctgcaagcg tttggcgcgt
     1501 gcctcagtgt ccttaatttc caggatctgc tcggcctcgg ggtttataaa caacacctcg
     1561 tttgacctac ccaacaccaa aatggcgtcg gtagtggaat taagaatggc ggagaatagc
     1621 tgttcttttt cgcgtatctg ggtgtcggcc gagcttttgt acagggccat ctccaaaagg
     1681 ttcgccagct cccgctcctt aaagggttta aggatatatc ccaggggatg ggcgtcgcgc
     1741 gcgcggtcca gggtaaggcc gtcggtgtag gcggaaagaa agataaccgg aatgtgaagc
     1801 tctgtgtaaa tgatgcgtgc gacttcgatg ccgtccatgg ggccttggat aaccacgtcc
     1861 atcaggataa ggtccggttt ttccgtgcgg atgccagcaa gtgcggtctc tccgtcggat
     1921 gcgcttccgc atacctgata gcccattcgt tcaaggcgcc gtttgagatc tattgcgatg
     1981 atgcgttcgt cttctacgat gtatatccgt tcgtttttca tgcaaacagc ttgctaataa
     2041 ttatttccac gatgtcagac tgggttaaaa gacccgtttg tacgcgaagt gaacacattg
     2101 taacatagca ttcggcgcgg cgggtaaaaa taacgcggaa gttttccttt ggattgtctc
     2161 cctgcaagaa cggggggagg cgaccgtccc ggcgagcgct ttccataatc ctgtcaaaca
     2221 ccacatcaaa gtcggcttcc agatagataa cagagccctt gccggaaagt gcttgtttgg
     2281 ccgcctggtt
//
LOCUS       DAOQXQ010000257         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53267_length_2287_cov_2.726703, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000257 DAOQXQ010000000
VERSION     DAOQXQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53267_length_2287_cov_2.726703"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..454)
                     /locus_tag="PKH81_05035"
                     /pseudo
     CDS             complement(191..454)
                     /locus_tag="PKH81_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            696..1562
                     /locus_tag="PKH81_05040"
     CDS             696..1562
                     /locus_tag="PKH81_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNQ97436.1"
                     /translation="MLANLNDVLVPAKRGRYAVGLFNSVNLELARAIIQAAERLRAPV
                     ILGTAEVLLPFGPLEELSWFLVPMAKRASVPVVVHFDHGMSMQLCENALRLGFTSIMY
                     DASWLPYDENVRDTREMAAMAHGFGATIEAELGHIGSSENSAEGSAQTDMCQECFTDP
                     VQARDFVRSTGVDALAVAVGTVHGHYRAAPRLDFSRIASIAQAVEVPLVLHGGSGLSD
                     VDFARSIELGISKVNIFTDINCAAASACDRALRSGKTALTDLIPLEVEAVESSVAEKI
                     RLFGSEGKAGRY"
     gene            1584..2039
                     /locus_tag="PKH81_05045"
     CDS             1584..2039
                     /locus_tag="PKH81_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97437.1"
                     /translation="MERLQILKRKHVILFSVLIVLLIILAAMSVYALRGKGDYFFLIL
                     GAIHLYFAISYIRSFISSCTALEFTPDKIIFVKGKRRDERAYTAVRLIVEKPWGYVEV
                     VLDGKESLLLHGESPGGRKECDERRLSNIIRAEITSRSGESIPVQYRSR"
     gene            2065..>2287
                     /locus_tag="PKH81_05050"
     CDS             2065..>2287
                     /locus_tag="PKH81_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNQ97438.1"
                     /translation="MARKAFSAKDIPVAGPYSQAVEAGGFVYLSGQLPIVPSTGKLVE
                     GCVACQTTQCFANIKAVLEAAGLGFDDVVK"
BASE COUNT          549 a          482 c          591 g          665 t
ORIGIN      
        1 ccccagtttt agaaaccatt gcccgatatc tttccattca tcgaggcatt attaccatcg
       61 ttaaccgtaa taatggggag attgtcatag aagaagcatg ggggtatgga acgggagaaa
      121 ttgaaaaggg gcattatcag ccgggagagg gtatcatagg tagggttatt gaaactggta
      181 accccgtcgc gggtccacct gactcgacgc agcgggttcc cttaaagccc gcctttacaa
      241 cgtcttcaag atcaagatcc tctccttctt cacgtaaggt atcgaggaca gtcttttgcg
      301 aaagtcctcc caaaagaagc cgtgttgagt cagctttggg gtcgcatccg acgactatga
      361 catttcgtcc catttcagcg agtgcggcga cggtgttctg cgtcgttgtg gatttaccga
      421 taccgccttt tccgtaaatt gctatcttgc gcatgttcct ctcctggagg tggttttcgg
      481 cgtgtgcctt ttgtgcgttt gccgatacct tcctattgca aggagcgtgc caatcatcgc
      541 taattattct atttctatgt acaggaagga attaacgtgg gatagaaaga aagtccggac
      601 cggcaatctt acgtagatgt catttcagag tctgccaagc tacatttacg tattgttata
      661 ttcaggatat actcgtgctt cgggaggaac tacaaatgct tgccaatcta aatgacgtgt
      721 tagttcccgc taaaagaggt cgctatgcgg tgggattgtt caactcggtc aatcttgaat
      781 tagcccgtgc gattatacaa gccgcagagc gacttcgcgc gcctgtaata cttgggactg
      841 cagaggttct cttgcccttt ggaccgctcg aagaactatc ctggttcctg gttcccatgg
      901 caaaacgagc gtctgttccg gtggtggtgc attttgatca cggtatgtcc atgcagttat
      961 gcgagaatgc tttgcgatta ggttttactt ctatcatgta cgatgcttcc tggcttccgt
     1021 acgatgagaa tgtacgtgac actcgcgaaa tggctgctat ggcccatggc tttggcgcta
     1081 ctatagaggc agaattaggc cacataggaa gctccgagaa ttctgcagaa ggatctgcac
     1141 aaacggatat gtgccaagag tgttttactg atcctgtcca agcgagggac tttgttcgct
     1201 cgaccggtgt tgatgcactc gcggtagctg ttggtactgt tcacggacat tatcgggcag
     1261 ccccgcgact tgatttttct cgcattgcat ctatagcaca ggctgtggaa gtacctcttg
     1321 tattgcacgg aggatccgga ctctctgatg tggattttgc tcgatcgata gaacttggga
     1381 tatcaaaagt gaatatcttt accgatataa actgtgcggc ggcttcggcc tgcgaccggg
     1441 ctcttcggtc cggaaagaca gcccttaccg atttaatacc gttagaggtt gaagctgttg
     1501 aatcgtctgt cgcagaaaaa attcgacttt ttggatcgga aggaaaagcg ggccgctatt
     1561 aatttttata ctatgggagc tttatggaac gtttacaaat tttgaagcgc aagcatgtca
     1621 ttcttttttc tgttttaatc gttttgttga ttattcttgc cgcgatgagt gtgtacgcac
     1681 tacgaggtaa gggggattat ttctttttga tactaggagc gattcatctt tattttgcca
     1741 tttcgtatat acgaagtttt atttcttcat gtacagctct tgagtttact ccagataaaa
     1801 tcatttttgt gaaaggcaag cgacgggatg aaagagccta caccgcagta cggctcatcg
     1861 ttgaaaaacc ctggggatat gtagaggtgg tgttggatgg aaaagaaagc ctgcttcttc
     1921 atggagagag cccgggggga agaaaggaat gcgatgagcg acgtctttcc aatatcattc
     1981 gtgcagagat aacaagcaga agcggagaaa gcattccggt gcaataccgt tcgcgctaat
     2041 ttttttatag taaggggata gtatatggca agaaaagcat tttccgctaa ggacattccg
     2101 gttgcaggtc cgtattcaca ggcagttgaa gctggaggat ttgtgtatct ttctggacaa
     2161 ctgccgattg tcccttcaac aggcaagctt gttgaaggat gcgtcgcctg tcagactact
     2221 caatgttttg cgaatattaa ggccgtcctt gaagcagctg gtcttggttt tgacgatgtg
     2281 gttaagg
//
LOCUS       DAOQXQ010000258         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53348_length_2285_cov_2.846188, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000258 DAOQXQ010000000
VERSION     DAOQXQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53348_length_2285_cov_2.846188"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKH81_05055"
     CDS             complement(<1..379)
                     /locus_tag="PKH81_05055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNQ97439.1"
                     /translation="MKSDTTKIGMERAPHRSLFKAMGYTDEELSRPIVGVVNSHNEII
                     PGHIHLQAVVDAVKAGIRMAGGTPMEFPAIGVCDGIAMGHEGMRYSLVTRELIADSIE
                     CVAKAHAFDAIVMIPNCDKVVPGM"
     gene            complement(410..1504)
                     /gene="leuB"
                     /locus_tag="PKH81_05060"
     CDS             complement(410..1504)
                     /gene="leuB"
                     /locus_tag="PKH81_05060"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNQ97440.1"
                     /translation="MNYKITTIPGDGIGPDIVREGCRVLDVIGKKYGHSFTYTETLAG
                     GAALDATGEPLPKATLDACLASDAVLLGAVGGPKWDVLPGAKRPEKALLGLRAGMNVY
                     ANLRPALMFPQLSAACPLKPEIVAKGLDILIVRELTGGIYFGDRGRNDKGDSAWDTEA
                     YSIPEIERVVKIGFDSAMKRSKKLCVVDKANILETSRLWREVTEKMKASYPEVELSYM
                     YVDNAAMQLVRNPGQFDVIVTSNMFGDILSDEASQITGSIGMLASASLGDSGPGLYEP
                     IHGSAPDIAGKDIANPLATILTCAMLLRYSLGLGVEACMIETAVERVLDEGWRTADIA
                     GSARDREALADKLVGTKKMGDLVLKAIEAL"
     gene            complement(1522..2013)
                     /gene="leuD"
                     /locus_tag="PKH81_05065"
     CDS             complement(1522..2013)
                     /gene="leuD"
                     /locus_tag="PKH81_05065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNQ97441.1"
                     /translation="MKAHGHVFKYGDNVDTDVIIPARYLNTSDAKELAAHCMEDIDAE
                     FIKKVTQGDIMVADKNFGCGSSREHAPLAIKTAGVSCVIASTFARIFFRNSINIGLPI
                     LECSEAVAGIKAGDEVEVDFDSGVITNVSTGATFKGQPFPAFMQDIISADGLINQLKA
                     KLK"
     gene            complement(2056..>2285)
                     /locus_tag="PKH81_05070"
     CDS             complement(2056..>2285)
                     /locus_tag="PKH81_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNQ97442.1"
                     /translation="LQAMKEGLAKIFVEAGVAFSTPTCGPCLGGHMGILAKGERALAT
                     TNRNFVGRMVTPKARCIFPIRPLPPRAPLPA"
BASE COUNT          537 a          612 c          586 g          550 t
ORIGIN      
        1 gcatgccggg tacaaccttg tcgcaattag gaatcattac aatagcgtca aaggcatggg
       61 cctttgctac acactcaatg gagtcggcaa tgagttcgcg ggtaaccaaa gaatagcgca
      121 tgccttcatg gcccatggca ataccgtcgc atacaccaat cgcaggaaat tccattggtg
      181 taccacctgc cattctgatg cctgccttta cggcatccac aacagcttgc aagtgaatat
      241 gaccgggaat aatctcgtta tgggaattga caacgccgac gatgggccgt gaaagctctt
      301 cgtcggtata gcccatcgcc ttaaaaaggg aacgatgcgg cgccctttcc atgcctattt
      361 tggttgtatc gcttttcatg ctggaacctc cattgattct gtgtctctct tagagagctt
      421 cgattgcctt tagaaccagg tctcccattt tcttggtacc gaccagcttg tctgccagag
      481 cctcgcggtc ccttgcagaa ccggcgatgt cggcagttcg ccaaccttcg tccagaacgc
      541 gttctacggc agtttctatc atacacgctt caacgcccaa cccaagggaa tagcgaagga
      601 gcatggcaca ggtaagaata gtcgccaggg gattggcaat gtcctttccg gcaatatcgg
      661 gtgcggagcc gtgaatgggc tcatagaggc cggggccact gtcgccaagc gaggcagaag
      721 cgagcatacc gatagatccg gtaatctggc tcgcctcgtc cgagagaata tcgccaaaca
      781 tattgctcgt aacgatgacg tcaaattgtc cggggttacg caccaattgc atggcggcgt
      841 tgtcaacgta catatacgaa agttcaactt ccggatagga ggccttcatc ttttcggtta
      901 cttcacgcca caggcgactc gtttccagga tatttgcctt atcgactacg cagagttttt
      961 ttgaccgctt cattgccgaa tcaaaaccga tttttaccac gcgctcaatt tcgggaatgc
     1021 tatatgcttc cgtatcccag gcggagtctc ccttgtcgtt tcgaccgcgg tcgccaaagt
     1081 aaataccacc ggttaattcg cgaacgataa ggatatcaag acccttggct acgatttcgg
     1141 gttttagagg acaggctgca gaaagctgcg gaaacataag ggccgggcgt aaattagcgt
     1201 acacgttcat gccggctctc aacccgagca aagccttttc ggggcgtttg gcaccgggaa
     1261 gaacatccca cttgggtccg cccacggcgc caagaagcac cgcatcactt gcgagacaag
     1321 cgtccagggt agccttgggc aagggctctc cggtagcgtc aagagccgcg ccgccggcaa
     1381 gggtttcggt gtaggtaaag ctgtgtccgt attttttgcc gatgacatca agaacgcggc
     1441 aaccttcccg cacgatatca ggtccaattc cgtctcccgg tattgttgtt atcttgtaat
     1501 tcatgtgtat ctcccctgcc cttatttaag cttggcctta agctgattga tcaaaccatc
     1561 cgcagagatg atatcctgca taaatgcggg aaagggttgc cccttaaaag ttgcgccagt
     1621 actcacgttg gtgatgacgc cgctgtcgaa gtcaacctcg acctcgtctc ctgccttaat
     1681 accggcaacc gcctccgagc attccaaaat gggaagaccg atgttaatcg agtttcggaa
     1741 gaagatgcgg gcaaaggtag aagcgataac gcaggaaacc cctgcggttt tgatcgccaa
     1801 gggagcgtgt tcacggcttg agccgcaacc gaagttttta tccgcaacca tgatgtcgcc
     1861 ttgtgtaacc ttcttgataa actcggcatc gatgtcttcc atacagtgag ccgcaagctc
     1921 ttttgcatcc gaggtattaa gatagcgggc agggatgata acgtctgtat caacgttgtc
     1981 cccatattta aaaacgtgtc cgtgtgcttt catgagatac tccttatcgg cccatcacat
     2041 cgacgggatc ggtgattacg ccggtaatgg cgctcgtggc ggcaacggcc ggattggaaa
     2101 gatacacctc gctttcgggg tgaccattcg tccgacgaag ttacggttgg tggttgcaag
     2161 ggcacgctct cccttggcaa gaattcccat gtgtccgccc aaacacggtc cgcaggtggg
     2221 agtcgagaag gctacgccag cttccacgaa aatcttggca agaccttcct tcattgcctg
     2281 aaggt
//
LOCUS       DAOQXQ010000259         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53702_length_2275_cov_2.659910, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000259 DAOQXQ010000000
VERSION     DAOQXQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53702_length_2275_cov_2.659910"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..579
                     /locus_tag="PKH81_05075"
     CDS             193..579
                     /locus_tag="PKH81_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97443.1"
                     /translation="MKHSYHACIAGLLSIILMFPIFAEAAPAVVFSVTNFSTPPVEEL
                     PPPPEEPLASHFFQSPNGKKALFWGGIAGIVISIVITGAGIATIADEGANGMSQEGIQ
                     DGVLMVGIGTLLAAFSSVAARTGSRK"
     gene            complement(583..1827)
                     /locus_tag="PKH81_05080"
     CDS             complement(583..1827)
                     /locus_tag="PKH81_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ97444.1"
                     /translation="MKRCLCILSLVLLSLSSLRPEHLGRTVITSTSWEVEGFSPDGGF
                     ALASSFSIQGLPLDRSVLTLRSGYRWIDSGEGLSVPGLSLTGGYRIPLPPWLSFKPMA
                     GVYSDFAVIQDGIEPTVGITAGIQLSFLLHDRDYLDLVPSFSFPLMDGTPARFSLAFA
                     SRRETPWLIPVPRVKARLSLSDSVFSPDGDGTDDVLGIKAGASSPRYAVTWRLTITDD
                     EGVLWFHSEGEGPPPGRLSWDGVSKKGILVDAGQNLVVELTVVDVLGRTSVCSRSALV
                     DILVLKDGDRYKVRVPNIHFPADSADMSSVSGSLLEENRAILERLASLFSRFPEHTLT
                     VEGHANAVHWANPARYRQEQVELEELSLRRAQAVRLSLVMLGVMEKRIRVEGYGSRRP
                     LVDFADKNSVWKNRRVEFILSK"
     gene            complement(1824..>2275)
                     /locus_tag="PKH81_05085"
     CDS             complement(1824..>2275)
                     /locus_tag="PKH81_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97445.1"
                     /translation="GQTSRQDSFDPDGSGGIPTLTASYTVDPQVSAAFTLSSGSLVLQ
                     ASTGVTLVELFSFSCGLGLSLSRVGASLSIDAEEEIRIGGTLADYIEGSGSVAVHGEV
                     VGVHAWYAQMYLHGAMEMRLGSLVLSFPVIWVPETSLACGLYAGVRF"
BASE COUNT          651 a          598 c          565 g          461 t
ORIGIN      
        1 aaaagatttg cggaagaaac gcataacgat gtcttaagaa aaaatgcgat aatgtaccgc
       61 gaacattcta cgtacatcga aaatctccgt acaattacaa acaatccggc ataggcctcg
      121 atgggtccga ctggcatagc gtagattatt tcatcttgcc ggatcggcgg agatcattta
      181 gaatctactg atatgaagca tagctatcat gcctgtattg cagggctgtt gtctattatc
      241 cttatgttcc cgatattcgc ggaagccgct cctgccgttg tgttctcggt taccaacttt
      301 tccacaccgc cagtagaaga acttcctcct cctcccgaag aaccgcttgc ttcacatttc
      361 ttccagtcgc ctaacgggaa aaaagccttg ttctggggag ggatagcggg aattgttatt
      421 tcgattgtaa taacaggcgc cggtattgcc actatcgccg acgaaggtgc gaacggaatg
      481 tcgcaagaag gaatccagga tggggtacta atggtgggga tagggacctt gcttgcagct
      541 ttctcttccg ttgccgcgcg aacgggttca agaaaataat ccttactttg acaaaataaa
      601 ttccacccgc ctgtttttcc aaacgctatt tttatctgca aaatcaacca gaggtcttcg
      661 cgaaccatag ccttccaccc gaatcctttt ttccataacg cctaacataa caagggaaag
      721 ccgaaccgct tgagcgcgac gaagcgaaag ctcctccagc tcaacttgct cctgacggta
      781 gcgcgcggga ttagcccaat gaacggcatt cgcatgacct tctaccgtca gggtatgctc
      841 ggggaagcgt gaaaaaagag aagcaaggcg ttcaagaatc gcgcgattct cttccagaag
      901 tgaaccagag accgaggaca tatcagctga atcggccgga aaatgaatat tgggaacgcg
      961 aaccttatag cgatccccgt cctttaacac aaggatatcg accagggcag agcgcgaaca
     1021 cacggaggtg cgacccagca catcaactac ggtcaattca accacaagat tttgtcccgc
     1081 atccacgaga attcctttct tggatacacc gtcccacgaa aggcgacctg ggggaggccc
     1141 ctccccttcg ctgtggaacc ataatactcc ttcatcgtcg gtaatcgtca atcgccaggt
     1201 aactgcataa cgaggtgagc ttgcccctgc cttaatccct aacacatcgt cagtgccatc
     1261 cccatccgga gaaaaaaccg aatcgctcag cgaaagacga gccttaactc gaggaacggg
     1321 aataagccag ggggtctctc ggcgggaagc gaaggcaaga gaaaaacggg caggagttcc
     1381 gtccatgagc ggaaacgaaa aagaaggaac aagatcaagg taatcgcgat catgaagtaa
     1441 aaatgacagc tggatgcctg cggtaattcc gacagttggt tctataccat cctgaattac
     1501 cgcaaagtct gaatagacgc cagccattgg cttgaacgaa agccaggggg gaagcggtat
     1561 gcgataacca ccggttaagg acaagccggg aacggaaagg ccttcgccag agtctatcca
     1621 gcggtagccc gatcgaaggg ttagtaccga tcggtctagg ggaagcccct gaatggagaa
     1681 ggaactcgcc aaggcaaaac caccgtcagg agaaaaacct tccacctccc atgaggtcga
     1741 ggttatgaca gttctgccaa gatgttcagg cctcagcgag gaaagggaaa gtagtactaa
     1801 cgaaaggata cacaggcagc gcttcaaaat cgcacccccg cgtacaaacc acaggcaagg
     1861 gatgtttcag gaacccaaat aaccgggaag ctcagcacca aggatccaag gcgcatttcc
     1921 attgcaccat gtagatacat ctgcgcatac caggcgtgga cgccaactac ctctccgtga
     1981 acagctacac ttcccgagcc ttcgatatag tctgcaagag ttcccccaat cctgatctcc
     2041 tcctcggcat ctatggacaa ggatgcgccc acgcgagata gggagagacc aagaccgcac
     2101 gaaaaactaa agagctccac aagggtaaca ccggtggaag cttgaagaac caaagacccg
     2161 ctggaaaggg taaaggcagc gcttacttgt ggatccactg tatatgaggc agttagggtc
     2221 gggattcctc ccgatccatc cggatcaaaa ctgtcctgac gagaggtttg ccccg
//
LOCUS       DAOQXQ010000260         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53813_length_2272_cov_2.702751, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000260 DAOQXQ010000000
VERSION     DAOQXQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53813_length_2272_cov_2.702751"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKH81_05090"
     CDS             <1..431
                     /locus_tag="PKH81_05090"
                     /inference="COORDINATES: protein motif:HMM:NF023739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HNQ97446.1"
                     /translation="KQLVSITLDETDPAFPLTVSALPVSPLRAVRRLSGSFTDFYTGT
                     QFDSYSPDYLEIHLTDASLVANPMSLLRQKFPFLLSIRQGTSTQAEHQRDQGMNEMPL
                     GDKRDPLSDFSRFIDLLYGEKDPEKELLFQSLLKECLDEA"
     gene            421..>2272
                     /locus_tag="PKH81_05095"
     CDS             421..>2272
                     /locus_tag="PKH81_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97447.1"
                     /translation="MRPEKLTIQNIGPFIGAHTIDFSTLGDIFLIFGKTGAGKTTIFD
                     ALCYAFYGNVPGSRKDLVRQMRSQGSLDTDEASVTLEFTLGLQRYRIIRVLPGERPGK
                     RGAKSQAIPEDVHLDQWLDSQWKDRTSTNKSETDKQIADLIRLSQEEFTRIVLLPQGE
                     FSRFLRQNSTDRKQILSKLFPVSEYSRVIERCRSDSRDAEFLLKETETALQSLQQRFN
                     PASAKDDLLIIQDKISDLRSQLETSGEKHRQLTALLERSRAIDGRVAERAEEIQKLAD
                     LERQTDSIAAAESRLASARKAAPLIARLESLHLLRHSIENGQHELTELQTTISRTEKD
                     LALLESLYSDQEKRHTERDTLVLRKEKLSVATGIAQKILEEREEQSTTAHLVTELTAE
                     LKAIASNSATLTERLELLSREGESLDQCMIVHSEAKNRLDTIKLLKELSEDHRKALLS
                     RDSYRITYTKSHTIRDELDADLAIAKAEHQEIEQLIQTQGLSEQARDLALLLHEGSPC
                     PVCGSLTHPKPAQNSTLDAIPLQSRRDAAKRRIADLEERKAQANENIASVEASLSAVE
                     ERIANNLSRCASLLSASSILDVDPSVLEDIPRIEKLLSDSVDSMQKAQDAL"
BASE COUNT          636 a          619 c          508 g          509 t
ORIGIN      
        1 ttaaacagct cgtgtcgatt acccttgacg aaaccgatcc cgcctttccg cttaccgtaa
       61 gcgcccttcc cgtttccccg ctcagggcgg ttcgccgtct cagtggctct tttaccgatt
      121 tctatacagg aacccaattc gactcctata gtcccgatta tctggaaata caccttaccg
      181 atgcatcgct tgtggccaat ccgatgagcc tgttacggca aaaatttccc tttctcctga
      241 gtatccgtca gggaacatcc actcaggcag agcatcagag ggaccaagga atgaacgaga
      301 tgccccttgg ggacaaacga gacccgcttt ccgatttttc ccgttttatc gatctgttgt
      361 acggagaaaa agacccggaa aaagaattac tcttccaaag cctgctcaag gagtgtcttg
      421 atgaggcctg aaaaactaac catacaaaac atcggcccct ttatcggcgc ccacaccata
      481 gatttttcca ctctgggaga catcttcctt atctttggaa aaacaggcgc cggtaaaacc
      541 accatctttg acgcactctg ttacgccttt tacggaaatg ttccgggaag cagaaaagac
      601 ctggttcgcc agatgagaag tcagggatcc ctggatacag acgaagcttc tgttaccctt
      661 gagtttaccc ttggcttaca aagataccgc attatacgag tccttccggg agagcggcca
      721 ggaaagagag gcgccaaaag tcaggccatt cccgaagatg tccaccttga ccaatggcta
      781 gactcccaat ggaaggatcg caccagtacc aataaaagcg aaacagataa gcagattgcc
      841 gatcttattc gcttatctca ggaagagttt acccgcattg ttctgttgcc ccagggagag
      901 ttttcccgct ttcttagaca gaactccacc gatcgcaagc aaattctgtc caaactattt
      961 ccggtgtcgg agtattcacg ggtaattgaa cgctgtcgct cagattcacg ggatgctgag
     1021 ttcttgctta aagaaaccga aacagccctt cagtctctgc aacagcgttt taatcctgca
     1081 tccgccaagg acgacctcct gataatccag gataaaatat ccgacttgag atcgcagctt
     1141 gagacatccg gggaaaaaca tcggcagctc accgccttac tcgagcgttc gcgggcaatc
     1201 gatgggcgag tggcagagag agcggaagag atacaaaaac tggcagatct tgaaagacaa
     1261 acggactcaa tagccgccgc tgaaagccgc cttgcgtcag cgagaaaggc cgcgcctctt
     1321 atcgctcgcc ttgaatcgct ccacctgtta cggcactcaa tagaaaacgg acaacacgag
     1381 cttacagaac tacagacgac tattagccgt accgaaaaag atcttgcact ccttgaatct
     1441 ctgtatagcg atcaagaaaa aaggcacact gaacgggaca ccctcgtgct caggaaggaa
     1501 aagctttctg ttgccaccgg catcgctcaa aaaattttgg aagagcgcga ggagcagtct
     1561 actacggctc atcttgttac cgaacttacc gccgaactaa aagccatcgc ttccaatagc
     1621 gcaacattga ctgagcgcct tgagcttctt tccagggaag gagaatccct tgaccaatgt
     1681 atgatcgttc attcagaagc aaaaaacaga ctggatacaa tcaaattact taaagagcta
     1741 tcggaggatc atcgcaaagc actattgagc agggactcct accgaatcac gtatacaaaa
     1801 tcacatacca ttcgcgatga acttgatgcg gaccttgcga tagcaaaggc tgaacatcaa
     1861 gagattgaac aactcattca aacacagggg ctttcagagc aagcaagaga tcttgcgctg
     1921 ttattacacg agggaagtcc ttgcccggtg tgcggatctc ttactcatcc caagccggct
     1981 caaaactcaa cccttgatgc aattccgctt caatcgcgtc gcgacgccgc aaaacgacgc
     2041 attgccgatc tggaggagcg caaggcacag gctaatgaaa acatcgcctc cgtagaagca
     2101 tctcttagtg cagtagagga gcgaatcgcc aacaaccttt ctcgctgtgc atcccttctt
     2161 tctgcctcat caatcctgga tgtggaccct tcggtgctcg aagatatccc tcgcatcgaa
     2221 aagcttctat ccgattccgt agactcaatg caaaaagcac aggacgccct gg
//
LOCUS       DAOQXQ010000261         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_53955_length_2268_cov_2.649345, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000261 DAOQXQ010000000
VERSION     DAOQXQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53955_length_2268_cov_2.649345"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKH81_05100"
     CDS             <1..249
                     /locus_tag="PKH81_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNQ97448.1"
                     /translation="SSKAKHAAAMMKITAEDILELKVCNGIIGEPAGGAHLDPKATSK
                     AIREVIIKDLKDLMPRNPAVLVRYRNQKIRKFGHFTEE"
     gene            complement(262..1533)
                     /locus_tag="PKH81_05105"
     CDS             complement(262..1533)
                     /locus_tag="PKH81_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNQ97449.1"
                     /translation="MELVAPAGNLEKLEYAWQYGADAAYIGLKSFSLRVKADNFHGDE
                     FSRIKALKEEYAKRGQSKRLFCALNITFHDSDMDAFLAEADYFAQYPFDAFIIQDIGM
                     VPLLQTKFPSIPLHLSTQANCINSRSVRMYKDLGFKRVVLGRETSLTDIRRIKDSVPE
                     MEIECFVHGAMCIAYSGRCLMSAYMAGRSANKGLCSHSCRWEYRYVEEKERPGEYFPV
                     IEGDGFTTIFASKDLCMIDHLEDMKRAGIDSLKIEGRMKSLYYTALVTRAYRKALDAL
                     EGNITAESAKPFIAELDNVPHRETGTGFYYSREDADKTTRRESSAPYLLAGTIGKLID
                     GDKGLWEFISLNKITAGTELEFLGPDSPPYTDQLYTLINPANGEQRQWVCHGHPCLIH
                     TTAPVSSGWIVRARDPSWTEKKGTEQCSVPS"
     gene            complement(1533..>2268)
                     /locus_tag="PKH81_05110"
     CDS             complement(1533..>2268)
                     /locus_tag="PKH81_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ97450.1"
                     /translation="PVSALFLVPELALGKRVILYAHGGGFISGSPYASRSLCASLAHE
                     AASRILIPAYRLAPEFPYPTALEDLYNSYAWILKQGIPADEVYFAGDGAGANLALSLI
                     QYLKPRNIRLPAGCIAISPWVDLSCSSSSFTARKNPDPLFTKDIMSAQALQYTWQDNF
                     TNPGVSPLFGDFSFMPPLYIQCGSEEILLDDAKQLAERARTEGVATVLDIEEGMWHLF
                     QSIDSLTPRAHVAVRKIGQWVRSGGR"
BASE COUNT          602 a          514 c          552 g          600 t
ORIGIN      
        1 tcttcaaaag caaagcatgc agctgcgatg atgaagataa ccgcagagga tattttggag
       61 ctcaaggtgt gcaatggcat aatcggtgaa ccagcaggtg gcgcccattt ggatccaaag
      121 gctacgagca aggcaatccg cgaggttatt atcaaggacc ttaaggatct tatgcccaga
      181 aacccagctg ttttggttcg ttatcgcaac cagaagatcc ggaagtttgg tcatttcacc
      241 gaagaataat aactatggta gttagctggg gacagagcat tgctctgtcc cttttttttc
      301 ggtccacgag gggtcacgag cccgtacaat ccatccggaa gacactggtg ctgtggtatg
      361 aattaaacag ggatggccat gacaaaccca ttgcctttgt tctccgttcg caggattaat
      421 aagggtgtat aactggtctg tatagggggg agaatcgggc ccaagaaatt caagctctgt
      481 ccccgctgtg atcttattta gtgaaataaa ctcccaaagt cctttatcgc catcaatgag
      541 cttgccgata gttccggcta aaaggtatgg ggcagaggat tctcgtcgtg ttgttttatc
      601 agcgtcttct ctcgagtaat aaaagccggt acctgtttcc cggtgtggaa cgttatctaa
      661 ttctgcgata aaaggcttcg cggattctgc ggtaatgttt ccttcaagcg catcaagggc
      721 ttttcggtat gcacgggtaa caagtgcagt gtaataaagg cttttcattc ttccttcgat
      781 ttttagagaa tcaatacctg ccctcttcat gtcttccaga tggtctatca tacaaaggtc
      841 tttggaggca aaaatggtag taaatccgtc tccttcgata accggaaagt attcgcccgg
      901 acgctccttt tcctcaacat agcggtattc ccatctgcat gaatggctac acaaaccctt
      961 attcgcactt cttccagcca tgtacgcgct catcaggcat cgacctgaat acgctatgca
     1021 catagcgccg tgaacaaaac actcgatttc catctctggg acgctgtctt tgatgcggcg
     1081 aatatcagta agacttgttt ccctgccaag aacgactcgt ttaaagccta aatctttata
     1141 catcctaacg gagcgagagt taatacagtt cgcctgggta ctcagatgca gcggaatcga
     1201 aggaaatttt gtttgtagaa gggggaccat cccaatatct tgaataataa aggcatcaaa
     1261 ggggtattga gcaaaataat cggcttctgc caaaaaagcg tccatatcag aatcatgaaa
     1321 ggtgatgtta agcgcgcaaa aaagcctttt gctctgtccc cgcttggcat attcttcctt
     1381 caatgcttta attcgggaaa actcatcgcc gtgaaaattg tccgccttta cccggaggga
     1441 aaaacttttt aagccgatgt atgcggcatc ggctccgtat tgccaagcgt attcaagctt
     1501 ttcaaggttg ccagctggcg cgaccagttc cattagcgtc ccccggaacg aacccattgc
     1561 cctattttgc ggacagctac atgagcacgt ggggttagtg aatcgataga ttgaaacaag
     1621 tgccacattc cttcttcaat atctagaact gtggccactc cttctgttct ggcccgttct
     1681 gccagttgtt tggcgtcatc aaggagtatt tcctcagaac cgcattgaat atacaaggga
     1741 ggcataaacg aaaaatctcc aaatagcggt gatacgccgg gattggtaaa attgtcttgc
     1801 caggtatatt gaagtgcttg ggcgctcata atatctttgg taaagagggg gtcgggattt
     1861 tttcgggcgg taaatgagga gctggagcag ctcaaatcaa cccatggcga tatggcgatg
     1921 caacctgcgg gaagccgaat attgcgcggt tttaaatatt gaatgaggga cagagcaagg
     1981 tttgctccgg caccgtcgcc agcaaagtat acttcatcag ccggtatccc ctgttttaga
     2041 atccatgcat agctattata caggtcttcc agtgcggtag ggtagggaaa ctctggtgca
     2101 agccggtatg cagggataag tattcgtgat gctgcttcgt gggctaaaga ggcgcacaag
     2161 cttcttgacg catagggaga tccggaaata aaaccgcctc catgggcata aagaataact
     2221 cgttttccca gtgctaattc agggactaaa aaaagcgctg acactggg
//
LOCUS       DAOQXQ010000262         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_54063_length_2265_cov_2.521719, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000262 DAOQXQ010000000
VERSION     DAOQXQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54063_length_2265_cov_2.521719"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKH81_05115"
     CDS             complement(<1..223)
                     /locus_tag="PKH81_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97451.1"
                     /translation="MKRNVVIVSLIVVFLAIPLISVWAVWEGNAGIASSSDFPSAGMY
                     AKSDMFPRNTIVEIRNLETERTIRAVITGS"
     gene            complement(223..753)
                     /locus_tag="PKH81_05120"
     CDS             complement(223..753)
                     /locus_tag="PKH81_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97452.1"
                     /translation="MHSRIVSLVVAMCCIPVICALGATVTVSVVQNEKAPEVALEMSR
                     TVEDELLGQFFDLGHIVSNSDISFKGSLFSQKNFGIKEAAFGMADYLVLVLLSYGPGE
                     RKNDETGMIYAELQKLSWRVVQVQDSAILDEKTIEYTDKTVKDFDPYAAVRVPARQIA
                     SDSISIMERNSQGGKR"
     gene            868..1386
                     /gene="loaP"
                     /locus_tag="PKH81_05125"
     CDS             868..1386
                     /gene="loaP"
                     /locus_tag="PKH81_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HNQ97453.1"
                     /translation="MNYFALQVKTRAEDIFIKRALATLSEGDYGGIRLIFPRRKLPIR
                     RKGVIRQELAPVFPGYIFLEAETFQPGLYWHLRTTNGFYRFLPENAEPKPLEGKDLST
                     LKHFVSFGEVAGSSIVRFDENDRIQVQEGPLKGLEGQIVKVDKRKGRAKIKLDLYDET
                     FLVDLAFEVLTH"
     gene            1449..>2265
                     /locus_tag="PKH81_05130"
     CDS             1449..>2265
                     /locus_tag="PKH81_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNQ97454.1"
                     /translation="MLAREMASREIFGKVIAFLDDDRAKIGTSIDGIPVIGPIRETAK
                     LIRIGVRDEALIAIPTASPETLRDIYSVLKSAGFIRIRLLPGIAQIIEGTAHLIQTRE
                     IDPQDLLGRPPVAINLKKSLSYLRGKRVLITGAGGSIGSELARQLLSGGAERIYLFGH
                     GENSIYQINHQLRLLQEEGVGEKATIVPVIGDMKDGPYMRYILSQLRADVIFHTAAYK
                     HVPLMEQNPVAVVENNVFGTANLLEASLESNVKRFVLISTDKAVDPVSVYGASK"
BASE COUNT          628 a          556 c          499 g          582 t
ORIGIN      
        1 atgagccggt aataactgca cgaatggtcc gctctgtttc cagattccga atctctacaa
       61 tcgtatttcg gggaaacata tcacttttgg cgtacatccc tgcagaaggg aaatccgaac
      121 tggatgcgat tccagcgttt ccttcccata ctgcccatac agagataagc ggtatggcaa
      181 gaaatacaac tataaggctt actattacta cattgcgttt cattatcgtt ttcccccctg
      241 actgttccgt tccataattg aaatagaatc gctcgctatc tgtctcgccg gtaccctgac
      301 cgctgcatac gggtcaaagt ccttgacggt tttatcagta tattcaatgg ttttctcatc
      361 caggattgcc gaatcctgta cctgtaccac tctccaggaa agcttttgca attcagcata
      421 gatcataccg gtctcgtcat tttttcgctc gcctggccca taagataaca ggacaagcac
      481 tagataatct gccatgccaa aggctgcttc cttaatccca aagttctttt gcgaaaagag
      541 agaaccctta aacgagatat ccgaattgga aacgatatgt ccaagatcaa agaattgacc
      601 gagaagttcg tcttctacag ttcggctcat ctccagagca acttccggtg ccttttcgtt
      661 ttgtacaacg ctcaccgtaa ctgttgcacc aagggcacag ataaccggaa tacaacacat
      721 cgccaccaca agggaaacaa ttcgactgtg catgggaccc tccctattcc tcctgattat
      781 cggacaaaaa aagaaggtca atgagttttt tacacgcgaa aaatctctct attctctttt
      841 tttgaccttt atgatatcgt agtattcatg aattattttg ccctgcaggt aaaaacccgt
      901 gccgaggata tctttatcaa acgcgcgctg gcaaccctga gcgaaggaga ctatggcggc
      961 atcagactta tttttccgcg ccgcaagctc cccattcgca gaaaaggggt aatcaggcag
     1021 gaattggctc ctgtttttcc cggatatatt tttcttgagg cggaaacctt ccaacccgga
     1081 ttatactggc acttacgaac gacaaatggc ttttatcggt tcttgcccga aaacgcggaa
     1141 cctaaaccgc ttgaaggaaa agacctttca acgttaaagc attttgtatc ctttggagag
     1201 gtagccggat cctctattgt aagattcgac gaaaacgacc gtatacaggt tcaagaagga
     1261 cccctaaaag gtcttgaagg acagatagta aaggtcgata aacgaaaggg acgagcaaag
     1321 ataaagcttg atctctacga cgagaccttc ctcgtcgatt tagcctttga ggttttaacc
     1381 cattgataaa ccaggcggac agcgcagata cgcgcgtata tatcatcgga gcaggacttg
     1441 ccggcattat gcttgcccgg gaaatggcat cgcgcgagat atttggtaag gttatcgcct
     1501 ttctggacga tgatcgggcc aagattggta catccataga cggaattccg gttattggac
     1561 caatacggga aacagcgaaa ttaatccgca ttggagtaag ggacgaagcc cttatcgcaa
     1621 ttcccaccgc aagccccgaa acccttaggg atatctatag tgttcttaag agcgccggtt
     1681 ttattcgtat tcgattactc cccggcatcg ctcaaattat cgaaggcacc gcccacctga
     1741 ttcaaacccg cgagatagac ccgcaagatt tacttggcag gcctccggtc gctatcaatc
     1801 tgaaaaaaag cctttcgtat ctgcgaggaa aacgggtatt gataaccggc gccgggggat
     1861 caatcggcag cgaattagcc cgacaactcc tctcgggagg agcggaacgc atataccttt
     1921 ttggacacgg tgaaaactct atttatcaaa ttaaccacca actcagactt ttacaggaag
     1981 aaggtgttgg cgaaaaggcg acaatcgtac cggtaatcgg ggatatgaaa gatggcccct
     2041 acatgcgcta cattcttagc cagcttcgtg ccgatgttat cttccatacc gccgcgtata
     2101 aacatgtgcc gcttatggaa caaaacccgg ttgctgtggt ggaaaacaat gtgtttggaa
     2161 ccgcgaactt gcttgaagca tcccttgaat cgaacgtaaa acgatttgtt cttatatcta
     2221 cagacaaagc agttgatcct gtttcggtgt atggcgcatc caaga
//
LOCUS       DAOQXQ010000263         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_54634_length_2250_cov_3.227790, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000263 DAOQXQ010000000
VERSION     DAOQXQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54634_length_2250_cov_3.227790"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKH81_05135"
     CDS             <1..223
                     /locus_tag="PKH81_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNQ97455.1"
                     /translation="LTMNLYGYFYGGDNVIRLDKDYQYALIAGSKLKYLWILSRTTNI
                     PDDITESYLATARSIGYKTEELIWVYHGE"
     gene            complement(248..1501)
                     /locus_tag="PKH81_05140"
     CDS             complement(248..1501)
                     /locus_tag="PKH81_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HNQ97456.1"
                     /translation="MKLLTGPRKHIVLLWLCWVIALGLFQSMVMHRFPVRPVDRVLPW
                     TALETGRQEENVNARLAVPIFTERVAWDSEFYLSIAQFGYDDPAVRKVYQSGRGSTSL
                     NYAFMPGYPYTVRAVAAPFIAFGAPRDAATVIAALAVSLAGALLAALSLFSILCPNEG
                     TPDSKRAVWYMFVFPSAFFLAQVYTEALFMGLVLTAIAQARKGRWMVTALIASCAVLT
                     RAAGVALVPAIAVTSIRFLAKELSRARLEESTPYRPSVAQVTGILQAVFIPLGVFLIW
                     KFSFLGERFDIVESAFFGRSFAPIVSIRAWLSCFASIITWPSPRGAYYAIELASLILA
                     LVASAWGFRRHPDLVVFGLAVLSASVLSVATQGIVRYILSCPIIFMFLAQKGRSPLFD
                     RVWSLISILLLGFFALLFSLDMWVA"
     gene            complement(1564..>2250)
                     /locus_tag="PKH81_05145"
     CDS             complement(1564..>2250)
                     /locus_tag="PKH81_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97457.1"
                     /translation="IITGYRKNGNTLLGWNFFQEMSEFGGGSLDESGYFVTDSWWENP
                     STRMLMAIGTECGELPPVKDILTGALNVLERESVMHSGGYAHLGGQEAYRALASALSD
                     DASFPSGMPLPQLFERLMCIDDALMMLGEARASGALWLKEVASAFEKSAKGEAAALAR
                     KAAQCLEGERKIAEEIEALPQWREAGEKRALALADPSVRKKLVSGIVKAASLNQGASE
                     AIRKLLDTLR"
BASE COUNT          632 a          618 c          575 g          425 t
ORIGIN      
        1 acttaccatg aatctttacg gatacttcta cggcggggac aacgtgatcc gactggataa
       61 ggactaccaa tatgcactca tcgctggaag caagctcaag tacctatgga ttctctcacg
      121 aactacaaac atccccgacg acatcacgga atcctatctt gctaccgccc gatctatcgg
      181 ttataaaacc gaagaattaa tctgggtata ccacggagaa tgaccgagtt catcaaaaaa
      241 agataattca ggcaacccac atatccaggg aaaagagcag ggcgaagaat cccaataata
      301 aaatacttat cagcgaccac acgcgatcaa agaggggaga gcgacctttt tgtgcgagga
      361 acataaaaat aattgggcac gagagaatat agcgaacgat accctgcgtt gctacgctca
      421 atacggaagc ggatagaacg gccagaccga agacaacgag atctggatga cgacggaatc
      481 cccaagccga ggcaacgagg gcaagaatca gagaggcaag ctcgattgcg tagtaagcac
      541 cccgcgggct cggccaggta ataatcgagg caaaacagga aagccatgcg cggatcgaaa
      601 cgataggagc gaaggagcgg ccaaagaacg cgctctctac gatatcaaac cgctcgccaa
      661 gaaatgaaaa tttccatatc aggaacactc caagcgggat aaaaacagcc tgaaggatac
      721 cggttacttg tgcaactgat ggtcggtacg gagtgctttc ctcaagccgt gcgcgcgaga
      781 gctccttagc aagaaagcga atagaggtta ctgcaatcgc cggtacgagg gcaaccccgg
      841 ccgcccgggt aaggacggca cacgaagcga taagcgcagt taccatccat cgaccctttc
      901 tcgcctgagc tatcgcagta agaacaaggc ccatgaacag agcctcggta tacacctgag
      961 cgagaaaaaa cgccgaaggg aaaacgaaca tataccaaac ggctcgcttt gaatcagggg
     1021 ttccttcgtt agggcaaaga atcgaaaaga gcgacaaggc ggcaagtaaa gctcccgcaa
     1081 gggataccgc caaggcagcg atgactgttg cagcgtcccg tggggcacca aaagcgataa
     1141 agggagcggc aacggctctg accgtatatg ggtagcccgg cataaaggca taattaaggg
     1201 atgtggaacc cctgccgctt tgatacacct tgcgtacggc gggatcatcg taaccaaact
     1261 gtgcgataga taagtaaaat tccgaatccc aggcaacccg ctctgtaaag atcggtacgg
     1321 caagacgagc gttcacgttt tcttcctgtc tgccagtttc tagcgcggtc caggggagca
     1381 cccgatccac tgggcgcacg ggaaaccgat gcattaccat cgactgaaaa aggccaagcg
     1441 cgattaccca gcagagccac agaaggacta tgtgtttacg agggccggtt aataatttca
     1501 tacgcgtatg attatacgcc taaatacggc gttattgtgt aacaataacg ccgcgaggct
     1561 tcattagcgt agcgtgtcca aaagtttccg aatcgcctcg gaggcgcctt gattcaggct
     1621 ggctgccttt actattccag aaacaagttt cttccgtacc gaaggatcag caagcgcgag
     1681 agctcgcttc tcgcctgcct cacgccactg cggcaaggcc tcgatctctt ccgcgatttt
     1741 ccgctctcct tcaaggcact gcgccgcctt gcgagccagg gctgccgctt cgcccttagc
     1801 cgacttttcg aacgcggagg caacctcttt aagccacagc gcgccagagg ccctggcctc
     1861 gccgagcatc ataagcgcgt catcaatgca cataagccgc tcaaaaagct gcggaagggg
     1921 catccccgac ggaaaagacg cgtcgtccga aagcgccgaa gcgagtgcgc gatacgcttc
     1981 ttgaccgccc aggtgagcgt atccgcccga gtgcataacg gattccctct cgagcacatt
     2041 gagcgcacct gttaagatgt ccttaacagg cggtaattct ccgcactccg ttccaatcgc
     2101 cataagcatc cgggttgacg gattttccca ccagctgtct gtaacgaagt atcccgattc
     2161 gtcgagcgaa ccgccgccga actcgctcat ttcctggaaa aaattccatc ccagaagcgt
     2221 attcccgttc ttccggtaac cggtaattat
//
LOCUS       DAOQXQ010000264         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_54715_length_2248_cov_2.958504, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000264 DAOQXQ010000000
VERSION     DAOQXQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54715_length_2248_cov_2.958504"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKH81_05150"
     CDS             <1..468
                     /locus_tag="PKH81_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97458.1"
                     /translation="LVVSRVFHDSLRDGENLGDFFNRFLPVDTATIRLAAALFLDYVL
                     RHALDRGKSSPPCLPSILEADIGSNARVEALSGIVSLLQKCRPPVIWRLFLTSLTALL
                     QRSIRTGQATAAEIAVYSQWTSLLGESVSAVDTYNITPLATLERLSVAMGDSL"
     gene            507..1631
                     /locus_tag="PKH81_05155"
     CDS             507..1631
                     /locus_tag="PKH81_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97459.1"
                     /translation="MNSEQMQSLLSLVTEEYELLDAVLDSLSTGVIVCDSFHIMVQSN
                     KAAARILPMDTYESQERAVWTTIRDPEIAFFVAQTIEAEDTVMAREFSIQDGLNSRYV
                     SISVMPLVRSKSVRGTIITIEDITSKKNEELKSRRLESLASLTNLAATVAHEIKNPLG
                     SISIYVQLLRKTLNKIGVTAEGEAQSYLDVVDEEIERLNKIVVDFLFAVRPFNFEFAP
                     LDLNALVSSLVDFFSTECEQSGITLTVETDQSLPLINGDERFLRQVFTNLIKNSMNAL
                     PDGGVIRFTTSHTEEMVTVKVSDSGTGIPAQVISRIFEPYFTTKVDGTGLGLTMAYKV
                     IKEHSGDIRVQSQPELGTTFTVDLPVLRKARKMIEYEERQ"
     gene            1634..>2248
                     /locus_tag="PKH81_05160"
     CDS             1634..>2248
                     /locus_tag="PKH81_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97460.1"
                     /translation="MKFTILVIDDEKNIRTGLKAALEMDGYEVLLASDGKEGLDIAFA
                     NEVDLVITDLRMPGVTGEEVLRKITSETPGIPVIVLTGHGTVENAVEAMRSGAYDFLT
                     KPLNLDRLSLLVKRALQNRELVIQHRELEKELGDKKNFEKIIGKSPVMLKVFDVVKRV
                     APTKASILITGESGVGKELIADAIHNLSTRKDNPFVKVHCAALAE"
BASE COUNT          595 a          460 c          538 g          655 t
ORIGIN      
        1 cttgtggtgt cacgggtttt tcatgattct ttacgagacg gggaaaacct gggtgatttt
       61 tttaaccgtt ttctacctgt tgataccgcc actattcgtc ttgccgcagc cttgtttctt
      121 gattatgtgc tcagacatgc tttggatagg ggtaagagct ctcccccttg tttgccttcg
      181 atactggaag cagatattgg atcgaatgcc cgtgttgaag ccctctctgg tattgtttct
      241 ctactgcaaa agtgccgtcc tccggttatc tggcgtctct tccttacttc tcttacggcc
      301 cttttacagc gatcgatacg aacaggacag gcaacagccg cagagatagc cgtgtattca
      361 cagtggacca gcttgttagg cgagtctgtc tctgcggttg atacctataa tattactccg
      421 cttgctacgc ttgagcgact ttctgtggct atgggggatt ctctttgagg gagtttatac
      481 gccgcgcctt acaaaaaacg tctcgtatga acagtgaaca gatgcaaagt ctgttatcgc
      541 tcgttaccga ggaatatgag cttcttgatg cggtactcga ttctctttct accggggtta
      601 tagtctgcga ttcatttcac ataatggtac agagcaataa ggctgccgct cgaattcttc
      661 ccatggatac atacgagagt caagagagag cagtatggac aactatcagg gatcctgaaa
      721 ttgctttttt tgttgcacag acgattgagg cagaagatac ggtaatggcc cgcgagtttt
      781 cgattcagga tggtcttaac agtaggtatg taagtatatc tgtgatgcct ctggtaagaa
      841 gtaaaagtgt tcgtggcact attattacca ttgaagatat tacctcaaaa aagaatgagg
      901 agttaaaaag ccgcagactt gaaagtttag caagccttac caatctggcc gctactgtgg
      961 cccatgagat aaaaaatcca cttgggtcca ttagtattta tgttcagctt ttacgaaaaa
     1021 ccctgaacaa gattggtgtt accgcagagg gagaagcgca atcgtatctc gatgtggtgg
     1081 atgaagagat cgaaaggctt aacaagatag tggtggactt cctttttgct gttcgccctt
     1141 ttaattttga gtttgcgcct cttgatctta acgcccttgt ctcttctttg gtagattttt
     1201 tttctaccga atgcgaacaa tcaggtatta ctttaaccgt agaaacagat caaagcttac
     1261 cactgattaa tggtgatgaa cgttttctaa ggcaggtatt taccaatctg ataaaaaatt
     1321 cgatgaacgc cttgcctgat ggaggggtca ttcggtttac cacttcccat accgaggaaa
     1381 tggttactgt taaggtcagc gattcgggta ccggtattcc ggctcaggtt atctctcgga
     1441 tttttgaacc atactttact accaaggttg atggtactgg tcttggtctt actatggcct
     1501 ataaggtgat aaaagaacat tcaggggata ttcgagtcca gtcacagccc gaattaggta
     1561 caacctttac cgtcgattta cctgtcttaa gaaaggcacg caagatgatt gaatacgagg
     1621 agcgccagta atcatgaaat ttaccatttt ggttattgat gacgaaaaaa atattcgcac
     1681 tggtttaaag gcagcgttgg aaatggatgg gtatgaggtt ttacttgcaa gcgacggcaa
     1741 ggaaggtctt gatattgcct ttgccaatga ggttgatctt gttattactg acttgcgtat
     1801 gccaggggtt acgggcgaag aggtgttacg caaaataacc agcgagaccc caggcattcc
     1861 cgttattgtg ctaaccggac acggaacggt ggaaaacgcg gtagaggcaa tgcgatcagg
     1921 tgcctacgat tttcttacca aaccgcttaa tctcgatcgt ctatccctcc tggtcaaacg
     1981 tgccttgcaa aacagagaac tggttattca gcatcgtgaa cttgaaaaag agctgggaga
     2041 caaaaaaaac tttgagaaaa taattggtaa aagcccggtt atgctaaagg tttttgatgt
     2101 agtaaaaagg gtcgctccta ccaaggcatc gattttaata acaggggaaa gtggtgttgg
     2161 caaggaactc atcgcggatg ccattcataa cctgtcaacc cgaaaggata atccctttgt
     2221 aaaggttcat tgcgctgctc ttgcagaa
//
LOCUS       DAOQXQ010000265         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_54979_length_2242_cov_2.055784, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000265 DAOQXQ010000000
VERSION     DAOQXQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54979_length_2242_cov_2.055784"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKH81_05165"
     CDS             <1..610
                     /locus_tag="PKH81_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97461.1"
                     /translation="LFEQGKKSLLAAYPKSFIGALHVSSLEGNPEAGWPVYLDGIAFG
                     SPVPVVQGSDASIAFITQAGILSLYDMKGVVKQGFPLSLTGIFYSQPVFDGKSLWLIS
                     STGTLYRVALDGSYLSHKIPDFSAEQGFIIIHDIDKDDEGELFVGGDGAILCGYRQDF
                     TPLAGFPLPLWGIPNFSDINGDGRLDVMGTGLDGKLYAWQFR"
     gene            620..1702
                     /locus_tag="PKH81_05170"
     CDS             620..1702
                     /locus_tag="PKH81_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97462.1"
                     /translation="MFLRCAVPLMALILVTSCASLQDDRIASLPGSAHDGELGELERI
                     VISLDSPSLSENDRQALVGEFRAKLGELERIEVKETVFSARLEAWSGRFALLNGNRME
                     GQRRLGRSLSLVPGDIPALVLSIRLQEDMGKRLLAAREALRLYPESGELLLEEALALS
                     GAGRYREAVVSFDASLSLLPAYYRQSYGEARDTAWTLRDMDEGTGVVTRAVSAAPRVS
                     WDQLIEISLEKQGFLDFITGGKLWKSDTTFSALSRDGYIPSPSGSLPLRSSVVSRGEL
                     AWYLWRITLSRSGTLDTANRYSSRWSANPRLPSPIPDVIPSSPWFDAALGCVEGEIMN
                     LPDGRNFFPSEPASGSDAVKALNATD"
     gene            complement(1777..>2242)
                     /locus_tag="PKH81_05175"
     CDS             complement(1777..>2242)
                     /locus_tag="PKH81_05175"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HNQ97463.1"
                     /translation="KVGTVRRDPFAMLPFCGYNMGEYFQHWINLGKKSTEDKLPKIFF
                     VNWFRKNAEGKFMWPGYGDNSRVLAWIFERCDGKDNAVKTQIGWMPKDGALNVTGLDV
                     KPEAMKEILSVDVEGWKKEIADVRANHYPKFGDKLPKELMTEMEALEKRLDA"
BASE COUNT          463 a          528 c          599 g          652 t
ORIGIN      
        1 cctgttcgaa caaggaaaaa agagccttct tgccgcctat ccaaaaagct ttattggcgc
       61 cttgcatgtg tcttctcttg aaggaaatcc cgaagcgggt tggccggtat atcttgacgg
      121 cattgccttt ggctctccgg tcccggttgt tcagggatca gacgcttcta ttgcctttat
      181 tacccaagcg ggtatcttat cgctgtacga tatgaaaggc gtggtaaaac aagggttccc
      241 tttaagctta accggaattt tttatagtca gcccgtgttt gacggaaagt ctttgtggtt
      301 aatctcttct accggaacgc tctatcgcgt tgcgttggat ggctcgtatc tgtctcataa
      361 aattcctgat ttttcggctg agcaaggttt tatcattatc catgatattg ataaggatga
      421 tgagggagag ctttttgttg gaggggacgg ggcaattttg tgcggttatc gtcaagattt
      481 taccccgctt gctggtttcc ctttgccctt gtggggtatc ccgaatttct ccgatattaa
      541 cggagatgga cggcttgatg ttatgggtac tggcttggat ggcaagctct atgcctggca
      601 atttagatag gagtgttgaa tgtttttacg atgtgctgta ccccttatgg ctttgatcct
      661 ggtaacttcc tgcgcgtcct tacaggatga tcggattgct tctcttcccg gaagtgccca
      721 cgatggggaa cttggagagc ttgagagaat agtgatttcc cttgattcac cttccctgtc
      781 cgagaatgac cgacaagcgc ttgtaggtga gtttcgcgca aagttgggtg agcttgagcg
      841 gattgaggta aaagagactg ttttttccgc ccgacttgag gcctggagtg gccgctttgc
      901 cctgttaaat ggtaatcgaa tggaaggaca gcgacggctt ggtcgctctc tgtccctggt
      961 ccccggtgat attccggcac tggtattatc cattcgctta caggaagata tgggaaagcg
     1021 ccttttagcc gctcgtgagg cattgcgcct gtatcctgaa tccggtgaac tattgttgga
     1081 agaggctctc gccctgagcg gcgcgggtcg ttatcgggaa gcagttgtgt cgtttgatgc
     1141 ctccctgtct ttattacccg cttattaccg gcagtcttat ggtgaagcga gggatactgc
     1201 atggacactg cgagacatgg atgaaggtac tggtgtggta acccgtgccg tttctgcagc
     1261 cccccgcgta agctgggatc agttaataga gatatcactg gaaaaacagg gttttcttga
     1321 ttttattacc ggtggtaagc tttggaaaag tgatacgacc tttagcgcat taagtaggga
     1381 tgggtatatt ccctctccct caggctcctt accgcttcgc tcctcggtag tatccagggg
     1441 agagcttgcc tggtatttgt ggaggattac cttatctcga tcgggtacac ttgacactgc
     1501 caaccgctat tcaagcaggt ggtccgctaa tccgcggctt cccagcccga ttcctgatgt
     1561 aatcccttcc tctccctggt ttgatgcagc tcttggctgt gtggagggag aaataatgaa
     1621 tttacccgat ggacgcaatt tctttccatc tgagcccgcc agcggctcag acgcggtaaa
     1681 ggcgcttaac gctactgatt agttaattga tatgtacagt ataaaaaaaa gcccgcgtaa
     1741 ccgcgggctt tttttggcta tacgccatgt gatgccttag gcatccagtc ttttttccag
     1801 ggcttccatc tcggtcatga gctctttggg gagcttgtcg ccgaacttgg gatagtggtt
     1861 ggcgcggaca tccgcgattt ccttcttcca tccttcaacg tcaacagaga ggatttcttt
     1921 catggcttcg ggttttacat caagaccggt cacattaagc gctccgtcct tgggcatcca
     1981 gccaatctgg gtcttaactg cattgtcctt gccgtcgcag cgttcaaaga tccatgcgag
     2041 gacgcggctg ttatcaccgt atcccggcca catgaacttg ccttcggcgt tcttgcggaa
     2101 ccagttaaca aagaagatct tgggaagctt gtcttcggtg ctcttcttgc caaggttaat
     2161 ccagtgctgg aagtactcgc ccatgttgta accgcagaag ggaagcatgg cgaaggggtc
     2221 gcggcgaacg gtaccgacct ta
//
LOCUS       DAOQXQ010000266         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55288_length_2234_cov_2.446535, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000266 DAOQXQ010000000
VERSION     DAOQXQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55288_length_2234_cov_2.446535"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2234)
                     /locus_tag="PKH81_05180"
     CDS             complement(<1..>2234)
                     /locus_tag="PKH81_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97464.1"
                     /translation="ATMESGDLSGISAGVRLGTDYHISPALKASISASARHWAYTPKP
                     FASALSIGVGLSIDLTELLSPDTNIDVEKYEQEPVFPVFYSWYDENHFAKVRVQNREA
                     NDITEVRTYFYLEQYMSQPKLCASVRTLKKGESVELPLKAFFNESMLSLTERVNAPAR
                     VIVEYKSLGSTRRVEVPVDVPVYHRNAMNWQDDRRAAAFVSARDPAAMWFARYVSSVV
                     KDRFRQGVNSNVQYAAGMFEALNAFGLNYVIDPTSAYADNIGSSSIDFLQFPHQTLMY
                     RGGDCDDISILYCSLLEAVGIDTAFVTIPGHIFMAFSSGMSEEEARQTYYDPSQLIFY
                     QGMAWVPVEITLTKEGFSKAWRIGAKQWRDAASRGDARIYPMKDSWTVYHPVSVPGAV
                     SRFNLPADEKTALAFDTSLDAVVEREIRDQIRDHEAAIARSDTGNTRNRLGVLYGRYG
                     MLAAAREQFAIAAKKGSTHARVNLANVYFLEQNYRDALTEYNRVLASDSANSLALLGA
                     ARCYYELDEFNRADTLYAELGTRDAALTRNYAYLGSFFETRGRAWSLADRLVTTTWSM
                     PPISEGPQPRKQAYVGYALTELPDEAPRIERADSGLMSALSVAPAASSEDIFDFDRET
                     EAGREAVLPGKNDAATLRALERQLAGTTSGDDDPDESLDATPVMATSVTEEPIAPGLG
                     AGADDPEVERALSLAAAPALAAAPVPKVVATPAAQAVVADTVTPVAGGVAEPKVIAEP
                     EPVA"
BASE COUNT          505 a          573 c          678 g          478 t
ORIGIN      
        1 gagcaactgg ttcaggctca gcaattactt taggctcagc cacaccacca gcgacgggtg
       61 taactgtatc ggctactaca gcctgagctg caggagtagc cacaacctta ggaacaggtg
      121 ctgccgctaa ggcgggtgca gccgccagac ttaaggctcg ctctacttcg gggtcatccg
      181 ctccggcgcc aaggccggga gcgatcggct cttcggtaac ggaagtagcc attaccggag
      241 tagcatccag ggattcgtcc ggatcgtcgt cgccagaggt agttcccgct agttgccgtt
      301 ccagggcgcg aagcgttgcc gcgtcgtttt tccccggaag aactgcttcc ctacccgctt
      361 ctgtctctcg atcaaagtca aatatgtctt cggagcttgc ggcgggagca acggacaggg
      421 cggacataag gcctgagtcg gcccgctcga tgcggggagc ctcgtcggga agttcggtaa
      481 gggcgtagcc cacataggct tgcttacgag gctgagggcc ttcgcttatt ggaggcatac
      541 tccaggtggt ggtgaccaga cggtcggcaa gagaccaggc acgaccgcgg gtttcgaaga
      601 atgagccaag ataggcatag tttcgggtaa gggcggcatc ccgggtgcca agttcggcat
      661 agagggtgtc ggcgcgatta aactcgtcca gttcataata acagcgggcg gctcccaaga
      721 gggcaaggga gttggcactg tctgaagcaa ggacacggtt atattcggtt agtgcgtcgc
      781 ggtagttttg ctcaaggaag tacacgttgg ccaggtttac ccgggcatgg gtactaccct
      841 tttttgcggc aatggcaaac tgctcgcgcg cggcggcaag catgccgtac cgtccataca
      901 aaacgcccag gcggttacgg gtgttgccgg tatccgagcg ggcaatggcc gcttcgtggt
      961 cgcgaatctg gtcgcgaatt tcccgctcca caaccgcatc cagcgaagtg tcaaaggcaa
     1021 gggccgtctt ttcgtcggct ggcaaattaa aacgcgacac ggcaccggga acgctaaccg
     1081 ggtgatagac cgtccaggaa tccttcatgg gataaatgcg cgcgtcccca cgggaagcgg
     1141 catctcgcca ctgttttgca ccgatgcgcc aggccttgct aaagccttct ttagtaaggg
     1201 ttatttccac cggtacccag gccattcctt ggtaaaaaat aagttggctg ggatcgtagt
     1261 aggtttgacg tgcctcttcc tcgctcatcc cggaagaaaa ggccataaaa atatggccgg
     1321 gaatagtgac aaaggcggtg tctataccca ctgcttccaa aagggagcag tagagtatgg
     1381 agatgtcgtc gcagtcgcct ccgcggtaca tgagcgtttg atgggggaac tgcaaaaagt
     1441 cgatacttga tgagccgatg ttgtcggcat aggcgctggt ggggtcaatg acgtagttaa
     1501 gaccaaaggc attaagggcc tcgaacatcc ccgcggcata ttgaacgttg gagttgactc
     1561 cctggcggaa gcggtcttta accacagaag agacatagcg ggcaaaccac atggcagccg
     1621 gatcgcgggc ggaaacaaag gccgcagccc ggcggtcgtc ttgccagttc atggcgtttc
     1681 ggtgatacac cggcacatcc acggggactt ctacccttcg tgtagagcca aggctcttgt
     1741 attccacaat aacgcgggcc ggggcattaa cccgttcggt aagcgagagc atggattcgt
     1801 taaaaaaagc tttaagggga agctcgacgg attcgccttt tttgagggta cgaacagagg
     1861 cacagagctt gggttggctc atgtactgtt caagataaaa ataggtgcgt acctcggtaa
     1921 tgtcgtttgc ttccctgttt tgaacgcgaa ccttggcaaa atgattttcg tcgtaccagg
     1981 aataaaatac cgggaagacc ggttcctgtt cgtatttttc tacatcgata ttggtatccg
     2041 ggctcagtaa ttcggtcagg tcgatggaaa gccccacccc aatcgagagg gccgaggcaa
     2101 aaggtttggg ggtgtaggcc cagtggcggg cagaagcgga gatggatgcc ttgagtgcag
     2161 gcgagatatg atagtcggta ccgagcctga ctcctgccga aattcccgac aggtccccgc
     2221 tttccatcgt tgca
//
LOCUS       DAOQXQ010000267         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55356_length_2232_cov_2.403767, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000267 DAOQXQ010000000
VERSION     DAOQXQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55356_length_2232_cov_2.403767"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..296
                     /locus_tag="PKH81_05185"
     tRNA            224..296
                     /locus_tag="PKH81_05185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Val,seq:tac)
     gene            387..>2232
                     /gene="uvrA"
                     /locus_tag="PKH81_05190"
     CDS             387..>2232
                     /gene="uvrA"
                     /locus_tag="PKH81_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ97465.1"
                     /translation="MKKKADPDRTAGNLLIVKGAREHNLKNIDIELPRDKLIVISGLS
                     GSGKSSLAFDTIFAEGQRRYVESLSAYARQFLGRMDKPDIDYIEGLSPAISIEQKTTH
                     KNPRSTVGTVTEIYDYYRLLYARIGKPHCPECGKEIQEQSVDQIVSTVLSWPDGTKVQ
                     VLAPVIRAKKGEHQKVVQDARDAGFVRARIDGLLVSLDDDIKLDKQKKHTIEIVVDRI
                     VLSLEARKRLAESIETALSSSGGILMVNRQDSDGSESDVLFSQKSACPDCGISIPELQ
                     PRLFSFNNPFGACPDCAGLGMKQEFDPDLIIPDSSLSFEDGGIVPYNPDSAWNRSRFA
                     ALAKKYNFSLDTPYGKLPKKVRSIIMDGSGEDAIDFVYQNREGTGHFKYHQSWPGIVA
                     DLKRRYNETFSDAQKENLEKYMSIHECETCKGKRLKREVLAVTVCGTNIHDLSSLSVT
                     DSIKFFKEAQFSETEEHIARQILKEINARLMFMKNVGLDYLTLERKAATLSGGEAQRI
                     RLATQIGSSLIGVMYILDEPSIGLHQRDNQRLIDTLLYLRDLGNTVIVVEHDEQTLRT
                     ADHIVDLGPGAGIHGGYIVAQGTPEEVMRVKKSLTGQYLAGTLKIRKID"
BASE COUNT          633 a          464 c          519 g          616 t
ORIGIN      
        1 gagggcgaca tcgacgtgga ctacaatacg cccactgacc ccacgaagcg gtacgtcaac
       61 gccttcgtcg tcaccatcgg catggacgag aactatcgtc gcctatatag cgaggcgaaa
      121 tttctcggtc gggtgaccat agaggatgac gattaacgcc ccgttgagcg gagggtcttt
      181 tccgcttgac ttgcccccgt cctctcgcgt acagtagggc atcgggcgct tagctcagct
      241 ggtagagcgt ttggtttaca cccaaaatgt cgggagttcg atcctctcag cgcccaaagc
      301 cgttcccttc ggggaacggc ttttttatta atcctcgcct tactgtctcg attgaccgga
      361 attaaaacaa tggctattat ttgtacatga aaaagaaggc tgatcccgat agaacagcgg
      421 gtaacttatt aatcgttaaa ggtgcccgag agcacaatct taaaaacatt gatattgaat
      481 taccacgtga caaattgatt gttatttccg gtctttccgg ctcagggaaa agttctcttg
      541 cctttgatac tatttttgct gaaggacaga ggcgatatgt agaatcactt tccgcctatg
      601 cccgtcagtt tctgggacga atggataagc cggatatcga ttacattgaa ggactgtctc
      661 cagctatttc tattgaacaa aaaaccaccc ataaaaatcc tcgttctact gttggaactg
      721 ttaccgagat ttatgactat tatcgccttt tatacgcccg catcggtaaa ccgcattgtc
      781 cagaatgtgg taaggaaata caagagcaat ccgtcgatca gatcgtatcg actgtattgt
      841 cctggcctga tggaacaaag gttcaggttc ttgcaccagt aatccgggca aagaagggag
      901 aacatcaaaa ggtagttcag gatgcacggg acgcaggttt tgttagagcc cgcatagatg
      961 gccttttagt atcccttgat gatgatatta agctggataa gcaaaagaaa cataccatag
     1021 agattgtagt tgatcgaatt gttctatctc tggaagcgcg aaagcgtctt gccgaatcaa
     1081 tagaaaccgc gctttcaagt tcaggcggta ttttgatggt taatcgtcag gattcagatg
     1141 gcagtgaatc tgatgtgttg ttttctcaaa agagtgcatg tcctgattgc ggtatttcaa
     1201 taccagagtt acagccccgt ctgttttctt ttaataatcc ttttggagct tgtcctgatt
     1261 gcgcgggact tgggatgaaa caagaatttg atcctgattt aattatccct gattcttctt
     1321 tgtcttttga agatggcggt attgttccgt acaatcccga ttcagcttgg aaccgctcca
     1381 gatttgccgc tcttgccaag aagtataatt tttctcttga tacaccatat ggaaagttac
     1441 caaaaaaagt ccgatccatt attatggatg gttcaggaga ggatgcgatt gattttgtct
     1501 atcaaaacag ggaaggtacc ggccatttta agtatcatca aagttggcca ggaatagttg
     1561 ctgacctgaa acgtagatac aatgaaacgt tttctgatgc tcaaaaagaa aacctggaaa
     1621 aatacatgtc cattcatgag tgtgaaacct gtaaaggtaa aagacttaaa agagaagtgc
     1681 tcgcggtaac ggtatgcggc acaaatattc atgacttatc aagtctttct gttacagatt
     1741 cgattaagtt ctttaaagaa gcccagtttt cagaaaccga agaacatatt gccaggcaga
     1801 tactaaaaga aattaatgcg cgccttatgt ttatgaagaa tgtaggtctt gactacttaa
     1861 cccttgaaag aaaagctgca accctttctg gcggagaagc ccagcgaata agacttgcca
     1921 ctcaaattgg ttcaagtctt ataggtgtta tgtatattct tgatgagcca tcgattggtc
     1981 ttcatcaacg agacaatcaa cgcttgatag ataccctttt atatctgagg gatttgggga
     2041 atacggttat tgtcgtagaa catgatgagc aaacactcag aaccgcagat catatcgtag
     2101 atctgggacc tggagctgga atccatggag gatacatagt cgcacagggt actcccgagg
     2161 aagtgatgcg tgttaaaaaa agcttgacgg ggcaatacct ggcaggcacg ctcaagatcc
     2221 ggaagatcga tg
//
LOCUS       DAOQXQ010000268         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55386_length_2231_cov_2.749540, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000268 DAOQXQ010000000
VERSION     DAOQXQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55386_length_2231_cov_2.749540"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKH81_05195"
     CDS             complement(<1..509)
                     /locus_tag="PKH81_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ97466.1"
                     /translation="MKTVAGHPASLVSVQTKYCAGCGHGIIHRIICELVDEMGLREKA
                     IITNPVGCAIWADLYFDFDSVQPAHGRTPAAATGIKRVLNDHLVICYQGDGDLAAIGT
                     AEIIHAANRGEKFTVIFVNNAIYGMTGGQMAPTTLVGQKAATAPDGRDPVSQGMGYPI
                     RVIEMLATL"
     gene            complement(506..1585)
                     /locus_tag="PKH81_05200"
     CDS             complement(506..1585)
                     /locus_tag="PKH81_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNQ97467.1"
                     /translation="MSEKTQEQILMKGNEAIAEAACRAGCLAYYGYPITPQNELIGYM
                     AKRLPELGRIFIQAESEVSAINMVYGSASCGVRAMTSSSSPGISLKQEGISYAAGADI
                     PLVFVNIVRGGPGLGSIAPAQSDYFQSTRGGGHGDYHVICLAPASVQEACDLTYAAFD
                     MADTWRVPVMILGDGVIGQMMEGITLPPMRDLSSLPSKASWAVGNMKGRAARHITSIN
                     LVPAELEKLIRERFERYAAIAEKEVRYEEKNCQDAKVILVAYGTSARICQGALEKARA
                     KGLPVGLFRPISVWPFPSSQLSSYATPEMTYLSVEMSMGQMVEDIRLAVNGKAPVELL
                     AHSGGVLPTEEEIVSRIERILEGKA"
     gene            complement(1582..1809)
                     /locus_tag="PKH81_05205"
     CDS             complement(1582..1809)
                     /locus_tag="PKH81_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ97468.1"
                     /translation="MKRGKIEIDRERCKGCLLCVHACPFTLIAVDSSMNSAGVYPVTF
                     SSGGRCTACTSCYRVCPDCAITVYELEEGEV"
     gene            1980..>2231
                     /locus_tag="PKH81_05210"
     CDS             1980..>2231
                     /locus_tag="PKH81_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNQ97469.1"
                     /translation="MAIEILSTGKYVPANRVTNDDLSKTMDTNDEWIRSHTGIGARHL
                     ANDEEATSDLAYKAAMDAIERFAPGKAAQTIQTIDLVVIG"
BASE COUNT          582 a          605 c          492 g          552 t
ORIGIN      
        1 tccagggttg caagcatttc gataacacgt atcggatatc ccattccctg gctcaccgga
       61 tcgcgaccat cgggagcagt tgcggccttc tgcccaacaa gggttgttgg agccatctgt
      121 ccgccagtca tgccatagat tgcattattg acgaatatga cggtaaattt ttcgccccta
      181 ttggctgcgt gaattatttc tgccgttccg atcgcggcta aatcgccatc tccctgatag
      241 cagataacca agtgatcgtt taatacccgc ttaatacctg ttgccgccgc cggtgtgcgc
      301 ccatgggctg gctgaacaga atcaaaatca aaataaaggt cagcccaaat agcgcagcca
      361 accggattag tgataattgc tttttcacgt aagcccattt catcaacaag ctcgcaaatg
      421 atgcgatgaa taatgccatg tccacaccca gcgcaatact tggtttgcac cgaaacaagg
      481 cttgcaggat gtcccgctac tgtcttcatg cctttccctc caaaatccgc tcaatacggg
      541 atacaatttc ttcttcggtt ggcaaaaccc ctcctgaatg agctaagagc tctacgggag
      601 cctttccgtt taccgcaagg cggatatcct ccaccatctg ccccatactc atctctacag
      661 atagatacgt catctccgga gtagcatacg aagagagctg acttgaaggg aagggccata
      721 cactgattgg cctgaaaagt ccaaccggaa gcccctttgc acgagctttc tccaaagccc
      781 cctggcaaat acgcgcagag gttccatagg ctaccaggat gaccttggcg tcctgacaat
      841 tcttttcttc gtaacgcact tctttttccg ctattgccgc atatcgttca aagcgctcac
      901 gtatgagttt ttccaattct gcgggaacca aatttataga ggtaatgtgc cgggctgctc
      961 tccccttcat attgcccaca gcccatgatg cctttgatgg taatgaagaa agatctcgca
     1021 ttggcggcaa ggtaatacct tccatcattt ggccaatgac cccatctccc aaaatcatta
     1081 ccggaacacg ccaggtgtca gccatatcaa aggccgcata ggttaagtcg catgcttcct
     1141 gaacgcttgc tggtgccaga cagataacat ggtaatcacc gtgtccaccg cccctggtcg
     1201 actggaaata atcgctttgg gccggggcaa ttgaaccaag gcctggtcct ccccggacga
     1261 tattgacaaa gacaagtggt atatcagcgc cagctgcata ggaaatacct tcttgcttta
     1321 agctaatacc cgggcttgaa gagctggtca tcgcgcgcac gccgcaagaa gcggaaccat
     1381 agaccatgtt tatggcggac acctcgcttt ctgcctgaat aaatattcgc ccaagttcgg
     1441 gcaaacgctt ggccatatag ccgataagtt cattctgagg ggttattgga tatccataat
     1501 acgcaaggca tcctgcacgg caagcagcct cggcaatggc ctcgtttccc ttcatcaata
     1561 tctgttcctg ggttttttcg ctcatacttc gccctcctca agctcgtaca ccgttatggc
     1621 gcaatcggga cacacccgat aacaggaggt acacgcagta catcgcccgc cagatgaaaa
     1681 ggtaactgga tagacacctg cgctattcat ggatgaatca acggctataa gtgtaaaggg
     1741 acaggcgtgg acgcacaaaa gacaaccctt gcaccgctcc cgatctattt cgatttttcc
     1801 tcgtttcatt accccgaagt cctccccgtg aggtatacta tgacattagc tcataatttg
     1861 tcaaactgac atattgacaa ttttcgcata tgtgctattt ttacccctgc aactattttt
     1921 gacactactg gtctaacgaa ggttgcatct acaggaaaaa atcttcaagg agcactattg
     1981 tggcaatcga aatactctct accggtaaat atgtcccggc aaaccgggta actaatgacg
     2041 atctttcaaa aaccatggat accaacgatg aatggatcag gtctcatacc ggtattggag
     2101 cccgccatct tgcaaacgat gaagaagcga cgagtgatct tgcatataag gcagccatgg
     2161 acgccattga gcgatttgct cccggaaagg cggcacaaac catccaaacg atcgatttag
     2221 tggtgatagg c
//
LOCUS       DAOQXQ010000269         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55605_length_2225_cov_3.698618, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000269 DAOQXQ010000000
VERSION     DAOQXQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55605_length_2225_cov_3.698618"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKH81_05215"
     CDS             <1..933
                     /locus_tag="PKH81_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ97470.1"
                     /translation="FFMSVYLVSGLLKEPLSESVMDADAKHFALFLPQNRNYYFTDIL
                     NGAQKSARDSGAALSVHTLDSDGVALRMAAYSGLDGVVVCPDLDDDIIFDSLMNLSRR
                     RIPLVLVNHNIPADQPWPFVGTNNFDYGKKVGNLLINSGSSRINLVVVYSDKSPAIYA
                     ERELVEMGIHSVLSNRLESSITSYRTDLNPRDAEKIVYQLVRFQGAVNTIVFTDLNDT
                     IAGTQALVDLNQVGRIQVVGFGNDPAIRHFIQKGIVAGSLVVNPYVMGYQAVNSLAQL
                     AESGYTSTSVDTGIDVIHAENLSVSRPDRERRMP"
     gene            930..>2225
                     /locus_tag="PKH81_05220"
     CDS             930..>2225
                     /locus_tag="PKH81_05220"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNQ97471.1"
                     /translation="MKQNFYSSFKVQMLTNIIGVLFILMFSASYILFSTIRLQRIVDG
                     SFVQQRYLKDMQGKLDEFQRPLLEFLSSKSSNALSELLVMIQTIGTLIPSYDRLPSDT
                     ISLQEKEVYSLLRAYLDIAGNVIEEKRGMDIARYTSLYDEMEAVREYINQEIETISGL
                     RFAGQLSEYEAFIETSGKIQFWNLMFIIFISLFSLCILVHTVEKINRPMVNLSQMAAQ
                     LSAGNFEIEDIGMTRLHEIDHVVSAFNRMKKDIHSYIEEIRWQRNVEQEYMQERVRNM
                     KMEQMLRRMEVYTLQAQMNPHFLFNTLNTGIQLAIVEGADRTCAYMEHLAKLFRHNLR
                     EKNVIVPLRHEIEGLESYFYILGVRFPKNLDLVLDCPADILDDTKVPASVLQPLVENC
                     VVHAFRGREGINSIIVRVYRQDRRLCMSVADNGVGMDKET"
BASE COUNT          553 a          497 c          525 g          650 t
ORIGIN      
        1 ttctttatgt ctgtgtatct ggtgagcgga ttacttaaag aacccctgtc cgaatctgta
       61 atggatgcgg atgcaaaaca ctttgccctc tttttacccc aaaatcgcaa ttactatttt
      121 accgacatcc tcaatggagc ccaaaaatct gcccgggata gtggtgcagc tctttctgtt
      181 catacactgg actctgatgg ggttgccctg cgtatggcgg cgtattcggg tcttgatggc
      241 gtagtggtgt gtcctgatct ggatgacgac atcatttttg acagcttgat gaatctttct
      301 cgccgtcgca ttccccttgt attggttaat cataacattc ctgcagacca gccttggccc
      361 tttgttggaa cgaacaactt cgattatgga aagaaggtgg ggaatcttct tatcaattca
      421 ggttcctcac ggattaattt ggttgttgta tacagcgata agtcccctgc aatttatgca
      481 gagcgagagc ttgtggagat gggtattcac agtgtcttgt caaatcgcct tgaatcttcc
      541 ataaccagtt atcgtaccga tcttaatccc cgcgacgcgg aaaagattgt gtaccagctt
      601 gttcggtttc aaggtgcggt gaatacgatt gtctttactg atttaaacga tacaattgcc
      661 ggcacccaag cccttgttga ccttaatcag gttggacgca ttcaggtagt aggctttgga
      721 aatgaccccg caattcggca ctttattcaa aaaggaattg ttgccggctc cctggttgtc
      781 aatccatacg taatggggta tcaggcggtt aattccctcg ctcagcttgc cgagtcgggg
      841 tatacctcaa cttccgtaga tactggtata gacgtaatcc atgcggaaaa tctttcggtc
      901 tctcgccccg accgcgaaag gcggatgcca tgaaacaaaa cttttacagt tcgtttaagg
      961 ttcaaatgct taccaatatc atcggggttt tgtttatcct gatgttctcg gcatcttata
     1021 ttttgttttc tacaattcgg ttacaacgca tcgtagacgg aagctttgtc cagcaacggt
     1081 atcttaagga catgcagggt aaacttgacg agtttcaacg ccccctgctc gaattccttt
     1141 ccagtaaatc ctctaatgca ctttctgaac ttctggtgat gattcagact attggcacgc
     1201 tcatcccctc ttatgatcgc cttccttcag acacaatctc cttgcaggaa aaggaagtgt
     1261 attctttgct tcgcgcctat cttgatattg caggaaacgt aatagaagaa aagcggggga
     1321 tggatattgc ccgctatact agtctttatg acgagatgga agcggtacga gagtatatta
     1381 atcaggagat cgaaactatt tctggtttgc gcttcgccgg ccagctctcc gagtatgaag
     1441 cctttatcga aacatccgga aaaattcaat tttggaatct gatgtttatt atttttattt
     1501 cgcttttctc tctgtgcatc ctggtgcata cggtagagaa gataaaccga ccaatggtta
     1561 acctttcgca gatggctgcc cagctttccg cgggaaactt tgaaattgaa gatatcggca
     1621 tgacgcgact ccacgagatt gaccatgttg tgtcagcgtt taaccgcatg aagaaggaca
     1681 tccacagtta cattgaggaa attcgttggc agcgcaatgt cgagcaggaa tatatgcagg
     1741 agcgtgttcg caacatgaag atggagcaga tgcttcggcg aatggaggtg tataccttgc
     1801 aagcgcaaat gaatccgcat tttttgttta atacattgaa tacgggcata cagcttgcca
     1861 tcgtggaagg cgccgacaga acatgcgcat atatggagca cctggctaaa ctgtttcgcc
     1921 acaaccttcg agagaaaaat gttatcgttc ccctcaggca cgaaatcgaa ggccttgaat
     1981 catattttta catccttggt gttcggttcc ccaaaaatct ggaccttgtt cttgattgtc
     2041 cggcggacat tcttgatgac acaaaggtcc ccgcatcggt tcttcagcca ttggtagaaa
     2101 actgcgttgt gcatgcattc cggggccggg aaggaatcaa ttcaattatt gtgcgagtgt
     2161 atcggcagga tcggcgcctt tgtatgtcgg ttgcggataa tggcgtaggt atggataagg
     2221 aaaca
//
LOCUS       DAOQXQ010000270         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55742_length_2222_cov_2.053069, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000270 DAOQXQ010000000
VERSION     DAOQXQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55742_length_2222_cov_2.053069"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKH81_05225"
     CDS             complement(<1..507)
                     /locus_tag="PKH81_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNQ97472.1"
                     /translation="MKYELTITLSPEDQDNPERITEALVNELTKQNASPHPRATDITA
                     YILQKKSIDARHGRIKVYLKYTVYIGETPSASALNPVPSWRQADPAKQVVIVGSGPAG
                     LFAALTLLEQGVTPIILERGKETPERKRDIADITRLGSVNPDSNYCFGEGGAGTFSDG
                     KLYTRSNKR"
     gene            601..1563
                     /locus_tag="PKH81_05230"
     CDS             601..1563
                     /locus_tag="PKH81_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97473.1"
                     /translation="MNFSGFPQVKKRLCDVKIYFLILFCISSYVFADEGVLDQVSLSF
                     RLTTRAETQIRVAGEFIFPFLVGDSALTEGNNIRFVLSADVSPVSVNGMLETVWKPLA
                     VFEISAVSFAGSGWDIPLASGVSFNKPGLDRLGKPDGSARFEGSPFMGMVWGTQLGAA
                     VQFDLGALYPGKWTHVVFRSFNSLRYRALTCADKNDSWQYEHDIGEERNGWTYLATHV
                     LGYQMPFKLNVIALVFEQEKRLYDTPGKEVWGDSYSRFTLGLAGEYLFSDKLALGVLV
                     QIRTKKNYIDSSTQYDWYQMREVDRDDPRDFEFYRLAASLTWIF"
     gene            complement(1575..>2222)
                     /locus_tag="PKH81_05235"
     CDS             complement(1575..>2222)
                     /locus_tag="PKH81_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNQ97474.1"
                     /translation="CGFSIPQIRNILAGKNIATYFEERKKELEKEVQESSRQLSSIIN
                     YMESLGKDPTSVYEVVIKELPQVLVYSKRMIVESYNSYFTVIPKIGEEIAAANPDLVC
                     VTDPPYCFIIYHDGEYRDHDIDMEFCEAVTDWGIAPPGIVFKIMNHVPCAACILHRGS
                     YNRLPYAYTAACKWIEDNGYLIADNPRESYIDGIWNKPNEDDWLTELQIPLVSNS"
BASE COUNT          555 a          421 c          562 g          684 t
ORIGIN      
        1 acgtttgttt gaacgtgtgt atagtttacc gtccgagaag gttcccgctc ctccttctcc
       61 aaaacaatag ttcgagtccg gatttacgct tccaagccgc gtgatatctg cgatgtcccg
      121 ttttctttct ggggtttcct tgcctcgttc caggattatt ggcgtaacac cttgctcaag
      181 caaggtaagg gcggcaaaaa gacctgcggg tccagaacca acaatgacaa cctgcttggc
      241 agggtctgct tgtcgccagg aaggaacagg gtttagtgca gaagctgaag gcgtttcgcc
      301 aatgtagaca gtgtatttaa gataaacctt aatccgtcca tgacgggcat ctatggattt
      361 tttttgaagg atgtaggcag taatatccgt ggcgcgtggg tgcggggaag cgttttgctt
      421 ggtaagctcg tttacaaggg cttctgtaat tcgctcaggg ttatcctggt cttctggaga
      481 aagggtaatg gttaattcat atttcacggt aggacagtat agcttctatc agcagtcttg
      541 cctatgcggt agtctcgatt aaaaaacggt tttttgatag tatagaaatg gagtttgtat
      601 atgaattttt cggggttccc ccaggtaaaa aaaagactat gtgatgtaaa gatatacttc
      661 ttgattctgt tttgtatttc atcatacgtg tttgcggatg agggagtcct tgatcaggta
      721 agtctttctt ttcgtttaac tacacgagct gaaacacaaa tcagggtagc cggtgagttc
      781 atttttccct ttttggttgg ggattcagcc ttgacggagg gaaacaacat tcggtttgtg
      841 ttgagcgcgg acgtatctcc cgtttctgta aacggtatgt tggaaaccgt ttggaagcct
      901 ttagcagtgt ttgaaatatc agcggtaagt tttgcagggt ctggatggga catcccttta
      961 gcgtccgggg tgagttttaa taaacccggc cttgatagac ttggtaagcc agatggtagc
     1021 gcaaggtttg aaggctcccc ctttatgggt atggtgtggg ggacacaatt gggggctgct
     1081 gtacagtttg acctgggtgc attatatcca gggaaatgga ctcatgtagt tttccgtagt
     1141 tttaattccc tgcgttatag ggctttgacc tgtgctgata aaaatgattc ttggcaatat
     1201 gaacatgata ttggggaaga gcgaaatgga tggacatatt tagcgacgca tgtgcttggc
     1261 tatcagatgc cctttaagtt aaatgtaatc gcactggtgt ttgaacaaga aaaaagattg
     1321 tacgacactc ctggaaaaga ggtgtgggga gattcttact cccgctttac ccttggcctt
     1381 gcaggagagt atctattttc ggataagctc gctcttggtg tattagtaca gattcgaacc
     1441 aagaaaaatt acatagattc aagcacacaa tatgattggt atcagatgag agaagttgat
     1501 cgggatgatc ctcgtgattt tgaattctat cggcttgctg ccagcttaac ctggattttt
     1561 taggtagctt gttgctaaga gttggaaacc aaggggattt gtaattctgt aagccagtca
     1621 tcctcgttgg gtttgttcca aattccgtcg atatacgatt cacggggatt gtccgctata
     1681 agatagccat tgtcttcgat ccacttacat gcggctgtat aggcataggg aaggcgattg
     1741 tatgagcccc tgtgaaggat gcatgcagca caaggaacgt ggttcattat tttaaaaacg
     1801 atacctggag gagcaatccc ccaatcggta accgcttcgc agaattccat gtcaatgtcg
     1861 tggtctctgt attcaccgtc gtgataaatg ataaagcagt atgggggatc agtaacgcaa
     1921 acaagatcag gattcgccgc ggcgatttct tcgccaatct tggggattac ggtaaagtaa
     1981 ctattatatg attctactat catacgtttg ctgtacacca agacttgggg aagttccttt
     2041 attactactt catacacact ggttggatct ttgccaaggc tttccatata gttaataatc
     2101 gaggaaagtt gtcgggaaga ttcttgaact tctttttcca gttccttttt tcgctcttca
     2161 aaataggtgg cgatgttttt tccggccagt atatttcgta tctgtggaat ggaaaaacca
     2221 ca
//
LOCUS       DAOQXQ010000271         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_55985_length_2215_cov_3.218056, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000271 DAOQXQ010000000
VERSION     DAOQXQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55985_length_2215_cov_3.218056"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKH81_05240"
     CDS             complement(<1..661)
                     /locus_tag="PKH81_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97475.1"
                     /translation="MIKRCIAQCLLFVVTSVSIVCAVEVLPITTESWRFIDRSGSEVA
                     LDGVLPLAITSTPPRTEFDGISLLCDFSLPSEWKGPLGICLYKNNMSIKVFVNDVYID
                     TLGRPGPDFFFQPYITRGVLVPQSILASKNTLRMELWNDTGTYQLRMMNFWDEATYRT
                     AMNRFNFLDVQLPRFACMLLLFVSVYSLFLFINYRGRKESICLSMGSFFFAMYLLNVS
                     AF"
     gene            complement(814..1815)
                     /locus_tag="PKH81_05245"
     CDS             complement(814..1815)
                     /locus_tag="PKH81_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HNQ97476.1"
                     /translation="MALFESYERRINQILPVLKKYGISSVEECKTICDAKGVDVAALV
                     TGIQPIVFENGKWAYTVGAAIAIKKGEKEAVDIARTLGEGLQAFCIPGSVADDRKVGI
                     GHGNLAAMLLSDETKCFCFLAGHESFAAAEGAIGIAKSANRARKTELKVCLNGLGKDA
                     AQIISRINGFTYVQTQFDYYTGKLAIVKEIPYSKGERAAVRCYGADDVREGVAIMWQE
                     GVDVSITGNSTNPTRFQHPVAGTYKKETVDAGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAISEAMKK"
     gene            complement(1831..>2215)
                     /locus_tag="PKH81_05250"
     CDS             complement(1831..>2215)
                     /locus_tag="PKH81_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97477.1"
                     /translation="LVCDAINTAMRELFLQIVYGRSQSAFSEGGLVVGASLEDLGKGL
                     RSQIGTMFATKAKGPRYLEMAEGYIKRLGVDADGAIIGYEFVNLGKMMDAIKKGTDAN
                     EALKGATGTYGRFAEAAKYVDPRHE"
BASE COUNT          585 a          550 c          523 g          557 t
ORIGIN      
        1 caaacgcact tacatttaaa agatacatcg cgaaaaagaa tgatcccatg gacaggcaaa
       61 tactttcttt tcgtccccgg taattgataa acaggaaaag tgaatacacc gacacaaata
      121 acaatagcat acaggcaaat cggggaagct gcacatccag gaaattaaag cggttcattg
      181 cggtgcgata cgtagcttca tcccaaaaat tcatcatcct cagctgatac gtgccggtgt
      241 cgttccataa ttccattcta agggtgtttt ttgaggccag gatggattgt ggaaccaata
      301 cgcctcgagt tatatacggc tgaaagaaaa agtctggccc aggccgaccc agggtgtcga
      361 tatatacgtc atttacaaag accttgatgg acatgttgtt tttgtataga caaatgccta
      421 gcgggccttt ccattctgaa ggaagagaaa aatcacacag aagggaaatc ccgtcaaact
      481 cggttcttgg tggagtagat gtaatggcaa gagggagtac cccatcaaga gcgacctcgc
      541 ttccagatcg gtcaatgaat cgccaggatt cagttgttat gggaagcact tctactgcac
      601 aaacaataga cacagaagta actacaaaca agagacattg agcgatacaa cgtttaatca
      661 tacggactat agtagcacga atggagtttt tttcaggttt tttttactca tagaactgtc
      721 tgcgatataa agaaacccgc tgggcaacca gcgggtttct ttatatgaag aatcaggcaa
      781 agcctgattc ttcggaagaa gtaagacgaa ggcttacttc ttcatggctt cagaaatcgc
      841 aacagccaca gcaacagagg ctcctaccat ggggttatta cccataccga taagacccat
      901 catttcgacg tgagccggaa cagaagatga tccggcaaac tgtgcgtctg agtgcatacg
      961 acccatggtg tcggtcatac cgtagctggc gggacctgcg gccatgttat caggatgcag
     1021 ggttcgacca gttccgccac cagaagctac cgaaaagtac tttttacccg cgtcaacggt
     1081 ttccttcttg taggttcctg caacagggtg ctggaaacga gtcgggttgg tggagtttcc
     1141 ggtgatggaa acgtctacgc cttcctgcca catgatggca acgccttcgc gaacgtcatc
     1201 ggctccatag cagcgaacag cagcgcgttc ccccttggag tagggaatct cttttacaat
     1261 ggcgagttta ccggtgtaat aatcaaactg tgtttgtaca taggtaaagc cgttgatgcg
     1321 gctgatgatc tgtgctgcgt ccttgccaag gccgttaaga cataccttaa gctcggtttt
     1381 tcgtgcgcgg tttgcgctct ttgcgatacc aatggcgcct tctgcggcgg caaaagattc
     1441 gtgaccggca aggaagcaaa aacacttagt ctcgtcggaa agaagcatcg ccgcaaggtt
     1501 tccatggcca ataccaacct tacggtcgtc ggcaactgaa ccgggaatac agaaggcttg
     1561 gagtccttct ccaagtgtcc gagcgatatc gactgcttct ttttcgccct tcttgatcgc
     1621 gatggcagcg ccaacggtgt atgcccactt accgttttca aacacgatgg gttggattcc
     1681 ggtaacaagc gcggctacat ccacgccttt tgcgtcacaa atagttttac actcttctac
     1741 tgaagagatt ccatacttct tcagaacagg aagaatctgg ttaattctgc gctcgtagct
     1801 ttcaaacaat gccattattt cctccctttc ttactcgtgc ctggggtcga cgtacttggc
     1861 ggcttctgca aagcgtccat aagtaccggt agcgccctta agggcttcgt ttgcgtctgt
     1921 tcctttcttg atggcatcca tcatcttgcc caggtttaca aactcataac caataatggc
     1981 tccatcggcg tcgacgccaa ggcgtttgat atacccttct gccatttcca ggtaacgcgg
     2041 accctttgct ttggtagcga acatagtacc aatctgactt ctaaggccct ttccaaggtc
     2101 ttcgagtgaa gctccaacca caagcccacc ttccgagaaa gcggactggc tgcggccgta
     2161 cacaatctgc aagaacagtt cacgcattgc ggtattgatt gcatcgcata ccagg
//
LOCUS       DAOQXQ010000272         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56044_length_2214_cov_2.397869, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000272 DAOQXQ010000000
VERSION     DAOQXQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56044_length_2214_cov_2.397869"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..784
                     /locus_tag="PKH81_05255"
     CDS             176..784
                     /locus_tag="PKH81_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97478.1"
                     /translation="MREALSSADYRLSGEAMLALARLGDSRSQFAIGDILLASTNPFI
                     LVRGVQAMEVFGSTASIPILLDLLRNELLPAHIADETILALSTLMGVPRRFYYAYESY
                     VTDRSKAADLVSDALEEAFARCKKEDMALRAVITSFISDSSADSPFVRWVLDFGQGHT
                     GVYAALLVGVALDADLNRLEPFRFFLCFWAASAFRDRKLIEK"
     gene            810..2141
                     /locus_tag="PKH81_05260"
     CDS             810..2141
                     /locus_tag="PKH81_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ97479.1"
                     /translation="MKSMSPRYRVCSIFALLLVCSLCISCSGYIGYGVINWSVPEHGL
                     VAGDVVPVFIQSNIGKVYVIGSGKDRNKRIEVPLWQLTLYSSASKARRAARDMEAFRY
                     MYAISRTDGLPIRSQSDNTSRQIYRLRKDQKIRILRSGEGAPVVAGNAPLEGTWYEVL
                     TDDGTTGWCFSYNLTVYDERDQDALTAKDEEEGPDVLLDVLLSRPWYPDHYRTMIESN
                     RIDVSRINPMWGFFPGRESLVARLETADGAVSFPYSGISRVDDRLYRFDGTTLTVQVR
                     RRDSIMVQYTDQNGMPQVHYFASLDTTADALIEAELDRRSSLLNEIRNKGPSFTSGNY
                     GVLTFTQTASFLWSGYQILSPALIPSGSGGGGSVELRYFLHQSLTRDWDGVLSFQFDQ
                     APAPVNFLYDLSDQGLRLEQVSSANIKDSVVLSRNLNPTVLFFTPGTGDVR"
BASE COUNT          439 a          597 c          550 g          628 t
ORIGIN      
        1 tccagcgcca gcttgcgacg agtttcctgt tgatcgaact ccttgatttg agcgctcacc
       61 gcctgcaggg tctcaccggc gacattctcc gccggaaacg atttcagcgc cgtctgcaca
      121 agctcgttct gcccgccttt gcggcgaatt tcgtctctcc cgtgctatcc cgcttttgcg
      181 cgaagcgctt tccagcgcgg attaccggct ttcgggcgaa gcaatgcttg ccctcgctcg
      241 cttgggggac tcgcgaagcc agtttgccat cggagatatt ttgctagctt ccacaaaccc
      301 ctttatcctg gttcggggcg ttcaagccat ggaggtcttt ggctctaccg cctcaatacc
      361 catcttgctg gatctattgc gtaatgaact tcttcccgca catatcgccg acgaaacgat
      421 acttgccctg tccaccctga tgggggtacc ccggcgcttt tattatgcct acgaaagcta
      481 tgttaccgat cgctccaagg ccgctgattt ggtgagcgat gccctggaag aggcctttgc
      541 acggtgcaaa aaagaagata tggcattgcg agcggtaatt acctcgttta tctcagactc
      601 gtccgcggac agtccctttg tccgttgggt ccttgatttt ggccagggtc acaccggagt
      661 ctatgcggcc ttgctcgttg gagttgccct tgacgctgac cttaatcgac ttgaaccatt
      721 ccgcttcttt ttgtgttttt gggcagcctc ggcctttcgg gatcgcaagc ttattgaaaa
      781 gtaggtttcg atagggtatt gtgttgttca tgaaatcaat gtctccgcgc tatcgcgtat
      841 gtagtatttt tgctctcctt cttgtctgct ctctctgtat ttcctgttct ggctacattg
      901 gctatggagt aatcaattgg tctgtgcccg aacatgggct tgtcgcaggt gatgtagttc
      961 ccgtctttat tcaatccaat attggcaaag tatatgttat cggttctggc aaagaccgta
     1021 acaagcgaat tgaggttcct ctgtggcagt taacccttta ttcatccgcc tcaaaggccc
     1081 ggcgagctgc tcgcgacatg gaagcgtttc gctatatgta cgcaatttcc aggaccgatg
     1141 gattacccat tcgatcgcaa agtgataata cctctcgtca gatataccgg cttcgcaagg
     1201 accaaaaaat ccgtatcctt cgttcaggcg aaggcgctcc tgtcgtcgcg ggtaacgccc
     1261 cgctggaggg aacctggtat gaggtgctta ccgatgatgg caccaccggc tggtgttttt
     1321 cctataatct gacggtatac gacgaacggg accaagacgc cctgactgcc aaggatgagg
     1381 aagaaggtcc cgatgtcctc cttgatgtgc ttctgtcccg cccctggtat ccagatcact
     1441 atcggaccat gatcgaatca aatcggatag acgtttcccg aatcaatccc atgtggggtt
     1501 ttttccccgg aagggaatcc ctggtagcgc gactggaaac cgccgacggg gcagttagct
     1561 tcccgtatag tggcatcagt cgcgttgacg atcgactcta ccggtttgac ggtacaacct
     1621 tgaccgtcca ggttcgccga cgggattcca tcatggttca gtatacggac caaaacggca
     1681 tgcctcaggt gcattatttt gccagccttg atacaaccgc cgatgcttta atcgaagctg
     1741 aattggaccg acgttcctcc ttgcttaacg agattcggaa caagggccct tcttttacct
     1801 caggtaatta cggggtgctt acctttacgc aaactgcctc ctttctgtgg agcggctatc
     1861 aaatactttc tccggccctt attccatcag gctctggtgg cggcggctct gtggagctac
     1921 ggtatttctt gcatcaaagt ttaacccgcg attgggacgg agtcctttcc ttccagtttg
     1981 atcaagcccc tgctcctgtt aactttctct atgatttatc ggatcaggga ttacgtcttg
     2041 aacaggtaag ctcggcaaat atcaaggatt cggtagtcct ttcccgtaat cttaatccta
     2101 cggtgctctt ttttacgccc ggaaccgggg atgtgcgctg atggcttttg tacagttttc
     2161 ccgggtgtct cttgcctttg gaaacaggga tatccttgat tcagtatcgc ttaa
//
LOCUS       DAOQXQ010000273         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56340_length_2206_cov_3.868433, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000273 DAOQXQ010000000
VERSION     DAOQXQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56340_length_2206_cov_3.868433"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKH81_05265"
     CDS             complement(<1..743)
                     /locus_tag="PKH81_05265"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNQ97480.1"
                     /translation="MADKKKKKSASLGCLFWIAFILLILVLFFFNRNNIRSVMEKTGA
                     AELISGRSKTETRDTGPEGPKIPDITTDTPKAPEEPLVERPALEAEKPVAKTEKPAES
                     PKPVAEKPAVTTEKTEVKPAVKKTEAVKKEEPKAEPTRKATLFFVSIDSDGRILRREI
                     SRQIAQSDSPLSDTIKTLLRGTTAEESKKDIRSLIPGGTKLLSATVKDGVATLNMSEE
                     FQFNQYGIEGYLGQLAQVVFTATAFPTVTS"
     gene            799..>2206
                     /gene="ptsP"
                     /locus_tag="PKH81_05270"
     CDS             799..>2206
                     /gene="ptsP"
                     /locus_tag="PKH81_05270"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNQ97481.1"
                     /translation="MEGVRKLEGLSASRGIAIGPVFCFPDEDTAEISRYPINGEAVDA
                     EWSLFDTALETSRREITLLLDDRNREQSEILNAHLLMLSDPEFIPRIKRDLETRLLNV
                     EAVLQDAVEESSGILRSTGDAYLAERAVDIEDAFGRVMSHVAALRHGTQGKGLKRSST
                     TTFIPPGTILVARNLKPSQALSLKDSGISGIILEEGGATSHVAILARSWRIPAVMGVR
                     SFASQVEDGQTVILDADNGLVIVSPTTGQLSAYRVQSAAPGESGKEKETRIRAPSVTR
                     DGARMVLRANIALAAEAEDAIAEGAEGVGLFRSEFLFMGSLNLPDEETQLASYLEAAR
                     LLKDTPLVIRTLDAGGDKMIGEQLSLTEKNPLLGWRAIRFCLDRRELFSAQLRAILRA
                     SAEGDVRIMFPMISNVEELDAALDVLEEAKNECERRGQAYNRRIKTGIMIETPAAAVC
                     ADLLAKKADFMSIGTNDLI"
BASE COUNT          476 a          539 c          617 g          574 t
ORIGIN      
        1 gatgtaaccg ttggaaaggc cgtggcggta aaaaccacct gtgcaagttg ccctaaatat
       61 ccttctatac cgtattggtt aaactggaat tcctcactca tgttcaatgt agcaaccccg
      121 tctttaacgg tggccgaaag aagcttggtt cctccgggaa taagcgatcg tatgtctttt
      181 ttggattctt cggcggtggt acccctaagc agggttttaa ttgtgtcaga tagcggagag
      241 tcgctttggg caatctggcg cgaaatttct cgtcgcagga tgcggccatc ggagtcgata
      301 gacacaaaaa agagagtggc ctttcgggta ggctctgcct tgggctcctc tttttttacc
      361 gcttccgttt ttttaaccgc aggttttacc tcggttttct cggttgtaac cgcaggtttt
      421 tcggcaaccg gtttggggct ttcggcaggt ttttcggttt tagctaccgg cttctctgcc
      481 tccagggcgg ggcgttcaac caggggttct tccggggctt tgggagtatc agtagtaatg
      541 tcaggaattt tggggccttc gggtccagtg tctcgggttt ccgtttttga tcgaccggag
      601 atcaattctg ccgcgccggt tttttccata acagaacgga tgttgttccg gttaaagaag
      661 aacagaacca ggatgagcag gataaaggca atccagaata gacaacccag ggatgcagat
      721 tttttcttct ttttgtccgc catggcccca tgatacgccg attttcccgt gtatgcaagg
      781 gagggttttt acgctatagt ggaaggcgtg agaaagcttg aaggcttgtc ggcgtctcgg
      841 ggcattgcaa ttggtcccgt gttttgtttt cccgacgaag ataccgcaga aatatcccga
      901 tatcccatca acggcgaggc tgtggacgcc gaatggagcc tttttgatac tgctttggaa
      961 acctctcgca gggaaatcac cctcttgctg gacgatcgta atcgcgaaca aagcgaaatc
     1021 cttaacgcgc atctgttgat gctttctgat cctgagttta ttccccgcat caagcgggat
     1081 ttggagacgc gtttacttaa cgtggaagca gttcttcaag acgcggtaga agaatcttcg
     1141 ggaattcttc gctccaccgg cgatgcgtat ctggccgaac gggcggtgga tatcgaggat
     1201 gcctttggcc gggtaatgag ccatgttgcg gccttgcgac acggaacaca gggcaagggc
     1261 ttaaaaaggt cttctactac cacttttatt cctccgggaa ccatattggt ggcccgtaat
     1321 ctaaaaccct cgcaagcctt gtcgcttaag gattcaggaa tttccggcat tattctggaa
     1381 gaaggcgggg ctaccagtca tgtggctatc cttgcccgct cctggcgcat accggcggta
     1441 atgggcgttc gctcgtttgc ctcccaggtt gaagacggac agaccgtcat cctggatgca
     1501 gataatggct tggtcattgt atctcctacc accggacagc tctccgccta ccgtgttcaa
     1561 agcgcggcgc cgggtgaatc cggaaaggaa aaagaaacgc ggattcgcgc accctcggtt
     1621 acccgcgacg gtgcgcgcat ggtattgcga gcgaacatcg cccttgctgc ggaagcagag
     1681 gacgccattg ccgagggggc agagggcgta ggcctctttc gctccgagtt tttgtttatg
     1741 ggttcgctta atctgccgga cgaggaaacg cagctcgctt cttacctgga ggctgctcgt
     1801 cttcttaagg acacacctct ggttattcgt accctggacg ctggcggcga caagatgata
     1861 ggggaacaac tttcccttac agaaaaaaat cccttgcttg gctggcgggc tatccggttt
     1921 tgtcttgatc ggcgcgagct ttttagcgcc cagctcaggg caatccttcg cgccagtgcg
     1981 gagggggatg tcaggatcat gtttcccatg atctctaatg tggaagaact tgatgctgcc
     2041 cttgatgtat tggaagaagc aaaaaacgaa tgcgagcggc gtggacaggc atataaccga
     2101 cggataaaaa ccggtataat gatagaaacc ccggctgctg cggtgtgcgc ggacctcttg
     2161 gccaaaaagg cagattttat gtcgatcggc acgaatgacc tgattc
//
LOCUS       DAOQXQ010000274         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56395_length_2205_cov_2.889302, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000274 DAOQXQ010000000
VERSION     DAOQXQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56395_length_2205_cov_2.889302"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKH81_05275"
     CDS             <1..216
                     /locus_tag="PKH81_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNQ97482.1"
                     /translation="HFFRIIGTNAVYHEELLTKEWYIKTNVSGLFAQVISRLHHDQSL
                     SDLLDNRNIIEKLVMQDKPPQDKNPAE"
     gene            241..1515
                     /locus_tag="PKH81_05280"
     CDS             241..1515
                     /locus_tag="PKH81_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ97483.1"
                     /translation="MRANVLSVDIGTSSLKAAIITVDGYDEGIVRVRFPRSGARTTKD
                     WIQAFTEALELLKIGRQDVEELRAIVVSGNGPTLVGINDKGEAGPVLMWNDPLDFEAA
                     HKARETGPSLFIPRIIEYRGRYPEFASEGCRILSAPEFLLWKLTGNPVTILPTPRYES
                     TYWTAASLSEANIDGGLLPSFVHSGQKVGQWKPPHYAHPITAIAGGPDFFVALAGTGA
                     ILPGLACDRAGSSEGLNICLANRVSHPDIRVLPSVQEPYWNASYLLPETGFLFNTWRL
                     LSDQSDQSYPQLLQEIVHSPIIPPEGEAPHQGRQLVEGIAFSVRTAALTLKEATGFKG
                     EWRLSGGQAKNAIWNQLKADITGETFVLTEMTDGELMGNAVIAFHSMGRYKTLALAAK
                     KMIRVVNRYTPNRNLHNIYKEKYNRFMEKPSL"
     gene            1512..2153
                     /locus_tag="PKH81_05285"
     CDS             1512..2153
                     /locus_tag="PKH81_05285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ97484.1"
                     /translation="MNIFHLPAKVRALIFDIDSTLYTNEEWAFEQVDAQIRYFASLRG
                     MSVLEARTLIQAEQERVLMQTGAKPSLGNTLTAFGIPISESVRWRQALLEPGRFLGAD
                     QRLQATLAELATRFTLIACTNNPVLPARKTLQALGVDDFFPVCIGLDTTGVSKPHELP
                     FRLACTHAGASCAECLSIGDRYEIDIALPLELGMGGILVDGVEDVYRLPAILL"
BASE COUNT          520 a          604 c          538 g          543 t
ORIGIN      
        1 cattttttcc gcatcatcgg aaccaatgct gtatatcatg aagagttgct caccaaggaa
       61 tggtacataa agacgaatgt ctcgggtctt tttgcccagg ttatttcacg tctgcatcac
      121 gaccaatccc tctccgatct acttgacaac cgcaacatca tcgagaagct cgttatgcaa
      181 gacaaaccgc cgcaggataa aaacccggcc gaataattcg cgccaaggga gacgtctgct
      241 atgagagcaa acgtgttgtc tgtagatatt gggacctcat ctcttaaagc cgcaattatt
      301 accgttgatg ggtatgacga aggtatagtt cgcgttcgct ttccccgttc aggagcgcgc
      361 acgacaaaag actggataca ggcttttacc gaggcacttg agctccttaa gattggcaga
      421 caagatgtag aagaattgcg agccatagta gtgtccggaa acggcccgac cctggtaggg
      481 attaacgata aaggcgaggc cggacccgtt cttatgtgga acgatccgct ggactttgag
      541 gctgcgcata aagcccgcga aacaggtcct tccctcttta tcccccgcat tattgaatat
      601 cgcggaaggt atcctgagtt tgccagcgag ggatgccgca ttctctctgc cccggaattc
      661 cttttatgga agctcaccgg aaaccccgtt accatattac ctaccccccg ctacgagagc
      721 acctactgga cggccgcttc tctttcagag gcgaacattg acggtggcct tttaccttcg
      781 tttgtgcaca gcgggcaaaa ggttggtcaa tggaagcccc cgcactatgc tcatccaatc
      841 accgctattg ccggtggtcc ggatttcttt gtagcgcttg cgggaaccgg ggctatcctt
      901 cccggtcttg cctgcgaccg cgcaggctcc agcgaggggc tcaacatttg ccttgctaat
      961 cgagtatcgc acccggacat tcgcgtatta ccgtccgttc aggaacccta ttggaacgcc
     1021 tcctatctcc ttcccgaaac cggcttcctt ttcaatacct ggaggctttt aagcgatcaa
     1081 tccgatcaaa gttacccaca gctattgcag gagattgtgc acagccccat tattccaccc
     1141 gaaggcgaag ccccccatca gggccggcaa cttgtggaag gcattgcctt ttctgtgcga
     1201 actgccgccc ttaccctcaa ggaagccacc ggctttaagg gggaatggcg actgtccggc
     1261 gggcaagcaa agaatgcgat ctggaatcag cttaaggcag acattaccgg cgaaaccttt
     1321 gtccttaccg agatgaccga cggggaattg atggggaacg cggttatcgc ctttcactca
     1381 atgggtagat acaaaaccct cgctctggcc gcaaagaaga tgattcgcgt cgttaaccga
     1441 tacaccccaa acaggaatct acataacatc tacaaagaaa aatacaatcg ctttatggaa
     1501 aaaccctctc tatgaacatt tttcatctgc cagccaaggt acgcgctctt atttttgaca
     1561 ttgattcaac gctgtacacc aacgaggagt gggcctttga acaggtagac gcacagattc
     1621 gctactttgc ctccctccgc ggtatgtccg tcttggaagc ccgaaccctt atacaagccg
     1681 aacaagagcg ggtgcttatg caaaccggcg caaaacccag cttgggcaat acgcttaccg
     1741 cttttggaat tccgatttcc gaaagcgttc gctggaggca ggcccttctg gagcctggtc
     1801 gttttttggg agccgatcag cggctacagg caaccttggc agaactagcc actcgcttta
     1861 ctcttatcgc ctgtaccaat aatcccgtgc ttcccgcgcg aaagaccttg caagctctgg
     1921 gtgttgatga tttttttcct gtctgtatcg gtcttgatac caccggggtt agtaagcctc
     1981 atgagcttcc cttccgtctt gcctgcacac atgcaggtgc ctcttgtgcc gaatgccttt
     2041 ccattggcga tcgttacgag attgacattg ctcttcccct agagttgggt atgggcggga
     2101 ttctggtaga tggggtagaa gatgtctatc gtctgccggc aattcttctt taaaaattca
     2161 tcgaggcgat aaaacgttcg gcgaaaacgg gactgtcggc cagtt
//
LOCUS       DAOQXQ010000275         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56442_length_2204_cov_3.062820, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000275 DAOQXQ010000000
VERSION     DAOQXQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56442_length_2204_cov_3.062820"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /gene="rpoD"
                     /locus_tag="PKH81_05290"
     CDS             <1..1539
                     /gene="rpoD"
                     /locus_tag="PKH81_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNQ97485.1"
                     /translation="ELKKAADTERKRLVYSEKDSSIDDPIRLYLREIGKENLLTAEQE
                     VELSKKMEDGENIIKNVIKHSGMIIPEFYGIAQKAFSKIDPHESNKPRKELNEEMAEK
                     RRLKQLYGEVLKSVLPEMKQYMALKKKLYERDQITSIFDDEELIAVREKILPAIQETE
                     IQSEEIDRFSEKFIEATRKIREYRRRQEKKEKELRLSDYGALRNLGKRLAIARERAKL
                     ESELGISANDIKEIYTQIQVINRKLRKLEYDFENSVEQIISMAREIHRGRVMMKSAKD
                     RLINANLRLVVSIAKKYTNRGLHFFDLVQEGNIGLIKAVEKFEYRKGYKFSTYATWWI
                     RQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQRLGREPTDEEIAEQLGWNVSR
                     VKQVKNVAREPISLETPIGEEEDSLLGDFIEDKDVENPANQTAFTLLQEQLRSVLSTL
                     PPREQEVLKMRFGLDDGYSLTLEEVGLYFNVTRERIRQIEAKALRRLRHPRRSRKLKD
                     YLDN"
     gene            1558..>2204
                     /locus_tag="PKH81_05295"
     CDS             1558..>2204
                     /locus_tag="PKH81_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HNQ97486.1"
                     /translation="MVMTEVLDKLKALQEILAQKNVLEAKIKDTPTLLSTQEELLARL
                     KKGYIEKNDQYEKVRQNIVDLKSQLFEAESSREKSEKAMDAISTQREYEALDKEIKDA
                     GEREQFLRKDLQKDERLFQDLDEELKREESLIKQQEDELGERKATLSAEIASLEKELG
                     KLAKEEDKVSPGINPETRFKLERIIKSKQGVGIVAIHGNVCTGCHMILPAQFANEV"
BASE COUNT          719 a          428 c          530 g          527 t
ORIGIN      
        1 gagttaaaaa aggccgctga taccgaacga aagcgcttgg tatacagcga gaaagactca
       61 tctattgacg atcccatacg tctgtatttg cgagaaatcg gtaaggaaaa cctccttacc
      121 gccgagcagg aagtcgagct ttcaaaaaaa atggaagacg gcgagaatat catcaagaat
      181 gttataaagc attccggtat gattattccc gaattttatg gaattgccca aaaggctttt
      241 tcaaagattg atccgcacga aagcaataag cctcgtaaag agctcaacga agagatggca
      301 gaaaaacgcc gtcttaagca gctgtatgga gaggttctta aatctgttct ccccgaaatg
      361 aagcaataca tggcgctcaa gaagaaattg tatgagcgag accagattac aagtatcttt
      421 gatgacgaag aactcattgc tgttcgcgag aagatattgc ccgcgattca agaaaccgag
      481 attcaatcag aggaaatcga tcgtttttcg gagaagttta ttgaagcaac ccgaaagata
      541 cgagaataca gacgtcgaca agaaaagaaa gaaaaagaac tcaggctctc tgattacggg
      601 gcccttcgaa atttgggaaa gcgtcttgcc attgctcgag aacgggctaa actagaaagc
      661 gagttaggga tcagtgcgaa tgatataaaa gaaatttaca ctcagatcca ggttattaat
      721 cgtaagctgc gtaagcttga gtatgatttc gagaattctg tcgagcaaat catttccatg
      781 gccagagaga ttcaccgagg ccgtgtaatg atgaaaagtg ctaaagacag gctcatcaat
      841 gcaaaccttc gtcttgttgt atcaattgcc aaaaagtata ccaatcgagg ccttcatttc
      901 tttgatctgg tacaggaagg caatattggt ctaatcaaag ccgtagagaa attcgagtat
      961 cgcaagggtt ataagttttc tacctatgct acttggtgga tacggcaggc aataacccgg
     1021 tccatttcgg atcaagctag aaccattcgt gtcccggttc atatgattga acagatcaac
     1081 aaggtcgttc gtgaatctcg gcaattaatg caacgtcttg gacgtgaacc taccgatgag
     1141 gaaattgccg aacaacttgg atggaatgtc agcagggtaa aacaagttaa gaatgttgcc
     1201 cgtgagccga tctcccttga aactcctatt ggtgaggaag aagattcctt attgggagat
     1261 tttatcgagg ataaggatgt tgaaaatcct gccaatcaga ctgcctttac tcttttgcaa
     1321 gaacagcttc gcagtgtatt gtctacgtta ccgccacgcg agcaagaagt tctcaaaatg
     1381 cgctttggac tggatgatgg atactcactg acactcgaag aggtcggttt gtactttaat
     1441 gtaaccaggg aacgaattcg acagatagag gcaaaagcgc tcaggcgact gagacatcct
     1501 cgtcgcagca gaaaattgaa agattatctc gataactgat caagagggga aattgatatg
     1561 gtaatgactg aagtattgga caagctgaag gccttgcagg aaattttggc ccaaaagaac
     1621 gttcttgagg caaagattaa ggatacgcct acgcttttat ccacgcagga agagcttttg
     1681 gcccgtctta agaagggtta tatcgagaag aatgaccagt atgaaaaggt tcgtcagaat
     1741 atcgtagatc ttaaatctca gctttttgag gcagagagct cccgtgaaaa atctgaaaag
     1801 gcgatggatg ccatcagtac tcaacgtgag tatgaagccc ttgataagga aattaaggat
     1861 gcaggggagc gcgagcaatt ccttcgcaag gatctacaaa aagacgaacg cttgttccag
     1921 gatcttgatg aagaactaaa aagagaagaa tccctgataa aacagcaaga agatgaacta
     1981 ggggaacgaa aggcgactct ttccgcggag attgcatccc ttgagaagga actaggcaag
     2041 cttgccaagg aagaagacaa ggtttccccc ggaataaatc ctgaaacacg ttttaaactg
     2101 gaaagaatta ttaaaagcaa acaaggggtc ggtattgtag ccattcatgg taacgtctgt
     2161 accggttgcc acatgattct tcctgctcaa tttgccaacg aagt
//
LOCUS       DAOQXQ010000276         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56663_length_2199_cov_3.205690, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000276 DAOQXQ010000000
VERSION     DAOQXQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56663_length_2199_cov_3.205690"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /locus_tag="PKH81_05300"
     CDS             complement(<1..2127)
                     /locus_tag="PKH81_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNQ97487.1"
                     /translation="MEFTQELIDGLTVNEVAILKRVAEELKIQPAQVSAVVSLVAEGC
                     TIPFISRYRKEKHGSLDEVQVRDSDHLFKSYHNLETRRIEIIKGIFTQGKLTELLHAN
                     VMKAATLTELEDLWAPFKKKKKTRGMLAIERGLESLADLMEQKDKGAVDAAAPEYVRI
                     DEENTELSVHSADEAIQGAMDIIAERIAQDPDNRADIRAWYYKTGEYVVTGVGDEGAQ
                     KTSVYQMYWDYKEPLNQIKPHRVLAINRGERDKALDVKIEVDTDGAIDILTRKYPPAN
                     DYHRQSIEDGLIRLLSPAVVREIRSDLSDKADDHGITVFSENLKNLLMQQPIKGTRVL
                     GIDPGIRTGTKCAALDETGKYLGSFVIYQHKAAEGEAAVLKAIKDFNIQLVAIGNGTG
                     TREVQEVVAKVIGDHCPSVLYTVVDEDGASVYSASDVAREEFPELDLTIRGAISIGRR
                     LQDPLAELVKIDPKSIGVGLYQHDVNQKKLSETLDEVVGSVVNNVGVNLNTASFSLLK
                     YVSGINGTLAKKIVAFREAGGKIVSRDQLMKVPGMGPKSFEQCAGFLKIPESPDPLDN
                     SWVHPENYEAARVVWTLVQEKKDVTQDVRGDIKTKFSLGDQTITDIIDELKKPNRDPR
                     DGFPKPIMQKGVIAFEDLKEGMMVTGKIKNVVDFGAFVDIGIKETALLHLSEMSDQYV
                     KDPMDVVKVGDVKECRIIGLDLDRRRI"
BASE COUNT          476 a          613 c          515 g          595 t
ORIGIN      
        1 tatacgcctg cggtcaaggt ccaatccgat aatccggcat tcctttacgt ctcctacctt
       61 taccacgtcc atcggatcct taacgtactg atcgctcatc tcggaaagat gcaataaagc
      121 ggtttccttg atgccaatat caacaaaggc gccaaagtct actacgttct tgatcttgcc
      181 ggttaccatc atgccttctt taagatcctc aaaggcgata acgccttttt gcataatcgg
      241 cttggggaag ccgtcgcggg ggtcgcggtt cggttttttc agctcgtcaa taatgtcggt
      301 aatcgtctgg tctcccagcg agaatttggt ctttatgtcc ccacgcacat cctgggtaac
      361 gtcctttttc tcctgcacca gggtccagac cacccgggct gcctcgtagt tttcgggatg
      421 gacccagcta ttatcaaggg gatcgggact ttcagggatt ttaaggaagc cagcgcattg
      481 ctcaaaactc ttggggccca tgccaggcac cttcattaac tgatcgcgcg agacgatctt
      541 gccgccggct tcgcggaagg caactatctt cttggccagg gtgccgttaa tcccggacac
      601 gtatttaagc aagctaaagc tcgccgtatt caggttcact ccaacgttgt ttaccaccga
      661 gccgactacc tcgtccaggg tttccgacag tttcttctgg tttacgtcgt gttgatacaa
      721 gccaacgccg atactcttgg gatcaatctt gacgagctcg gcaagcgggt cctgcaaacg
      781 ccgcccgata gaaatggcgc cccggatagt aaggtcaagt tcaggaaatt cctcgcgggc
      841 tacatcgctt gcggaataca ccgacgcacc gtcttcatca accacggtat aaaggaccga
      901 aggacaatga tcgccaatca ccttggccac cacttcctga acctcgcgcg ttccggtacc
      961 gtttccgata gctaccaact gaatattgaa gtccttaatt gcctttaaga cggcagcctc
     1021 gccctcggca gccttatgct ggtagataac aaaggagccc agatattttc cggtttcatc
     1081 cagggccgcg cacttggtac ccgtgcggat gccagggtct atgcccaata cccgcgttcc
     1141 cttaatgggc tgttgcatca aaaggttttt aagattctcg ctaaatacgg taataccgtg
     1201 gtcatctgcc ttgtcgctca agtcgctgcg gatttcgcgt actaccgccg gagaaagaag
     1261 gcggatcaac ccgtcctcta tagattgtct gtggtagtcg ttggcaggag gatatttcct
     1321 ggtaaggata tcgatagcac cgtctgtatc aacctcaatt tttacatcca gagccttgtc
     1381 cctctcgcca cggttaatcg ccaggacgcg gtgaggctta atctggttaa gcggttcctt
     1441 ataatcccag tacatctggt acacgcttgt tttttgtgcg ccctcatccc caacgccagt
     1501 aaccacatac tctccggttt tataatacca ggctctaata tccgcgcggt tatcgggatc
     1561 ctgggcaata cgttcggcaa taatgtccat cgctccctgt atggcctcgt ctgcgctatg
     1621 aaccgaaagc tctgtgtttt cttcgtcgat gcgcacatat tctggagcgg cagcatccac
     1681 cgcgcccttg tccttttgtt ccatcaggtc cgcaagactt tccaggccac gttctatggc
     1741 aagcatgccg cgtgtttttt tctttttctt aaagggagcc cacaggtctt ccagttcggt
     1801 tagcgttgcg gccttcatca cattggcatg aagaagctcg gttagtttcc cctgtgtaaa
     1861 gatgcctttg attatctcga ttcgccttgt ttccaggtta tgatagctct tgaacaagtg
     1921 gtccgaatcc cgtacctgta cctcgtccag ggagccgtgt ttttcctttc tgtatcgcga
     1981 aataaagggg atggtgcatc cctcggctac aagcgagaca acagcggaca cctgagccgg
     2041 ctgtatcttt aattcttcgg caacccgttt aaggatcgct acctcgttca ctgttaatcc
     2101 gtcaattaat tcctgggtaa attccatagg ggggtattct accggattcg cgccctgccg
     2161 acaagggaaa aagtaaacct aaagctcttg acagtccct
//
LOCUS       DAOQXQ010000277         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56750_length_2197_cov_2.471055, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000277 DAOQXQ010000000
VERSION     DAOQXQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56750_length_2197_cov_2.471055"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKH81_05305"
     CDS             complement(<1..565)
                     /locus_tag="PKH81_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97488.1"
                     /translation="MSWQKIRAAPMRYRLFVMTLVISLAGCLAWAGEPGTLVFPKPGF
                     YRDVQLISVTAGQGIRLKLYLDGEQKSLSDFPLLLSVPSGEAKQFTLTITEYPLDPSA
                     EALRTKQFSWTIDRQRPDAPHLVLYPADGGALVGATVNEKASIFYRMYHPLSGATSRG
                     ILSPDNRPFLPDGAVICVWAQDSAGNSG"
     gene            complement(562..1173)
                     /locus_tag="PKH81_05310"
     CDS             complement(562..1173)
                     /locus_tag="PKH81_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNQ97489.1"
                     /translation="MHKEFLSYDKVRNNALKMAWRIHQDGFIPDVIYVSLRGGAYLAN
                     VISEYFKIVRKGAHPVLYAAVVARSYSDVRQRDRVMVDGWTYSPEHLRHGDKILLVDD
                     IFDTGKTINHLVEILIEKGIPRADIKVAVHDYKYFSFHEEQLPIQPDYWCRKHEIPSP
                     DKDCWIHYMSHELIGLSEKELEEYYYRDDPELKPILSALNGAT"
     gene            complement(1241..>2197)
                     /locus_tag="PKH81_05315"
     CDS             complement(1241..>2197)
                     /locus_tag="PKH81_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNQ97490.1"
                     /translation="NDSTIPVASMGDSDSVQTGDIVFAVGSPLGYISSVTQGIVSAVG
                     RSGGPNGNINDFIQTDAAINQGNSGGPLVNIYGQVVGINAWIASSTGGSQGLGFSIPI
                     NNVKKAIDDFISTGKIRYGWLGVSLLNADKDIFEALGLKNKNGALASQVFIGSPADKG
                     GILPGDVVIALNGKEVKAVDQLVRDVGDLESGSKATFRVLRQGKEQNVTVLIEERNEQ
                     TVTDASKLWPGFMPIPLNKEVRDRLKISESVSGVVVSGVQPKSPAAVMGLQSGDVIVK
                     VNDRDITGLKDFYARLTDGKAKEIWFDVVREGQTVSTMRYKR"
BASE COUNT          599 a          562 c          487 g          549 t
ORIGIN      
        1 caccactatt gccagcacta tcttgagccc acacacagat aaccgctcca tcaggaagaa
       61 aggggcgatt atccggggaa agaataccac gtgaggttgc gccagagaga ggatgataca
      121 tacgataaaa aatggaagcc ttctcgttga cggtagcgcc taccagggct ccaccatccg
      181 ccggatataa gacaaggtga ggagcgtcag gacgctgacg atcgatagtc caggaaaact
      241 gcttcgtacg caaggcttct gcagagggat caagcggata ttcggtgatg gtaagggtaa
      301 actgtttagc ctcccctgat gggacgctca ataaaagcgg gaagtctgaa agtgatttct
      361 gttccccgtc aagatatagc ttcaacctga ttccctgccc ggcagtgaca gagataagct
      421 gaacatcccg gtaaaagcct ggttttggaa aaacaagggt gcccggctcc cctgcccatg
      481 caaggcatcc ggcaagggat ataacaagcg tcattacaaa gagacgatag cgcatcggcg
      541 cggcacgaat cttttgccag ctcatgtcgc tccgtttaac gcagacaaga tgggcttaag
      601 ttccggatca tcgcggtagt aatactcctc gagttccttt tcgcttaaac ctatcaactc
      661 atggctcata taatgaatcc aacaatcctt gtctggagat gggatttcat gcttacgaca
      721 ccagtaatcc ggttgaatgg gaagctgttc ttcatggaag gaaaaatatt tatagtcgtg
      781 aacagcgacc ttgatatcag cgcggggaat tcccttttcg atcagtattt ccaccaaatg
      841 gttaatggtt tttcctgtat cgaaaatatc atcaaccagc aaaattttat ccccatggcg
      901 cagatgttcg ggtgaatagg tccatccatc caccattacc cgatccctct ggcgtacatc
      961 cgaataggag cgtgcaacca ctgctgcata caaaaccgga tgtgcccctt ttcggactat
     1021 cttgaaatat tcactgataa cattcgccag gtatgcgcct ccccgaaggg agacgtatat
     1081 gacatcggga ataaacccgt cctgatgaat tcgccaagcc attttgaggg cattattgcg
     1141 tactttgtcg taacttaaga attccttatg catgagtaca acctcttata tctccactgt
     1201 aacccttttt taaggaatgc tcaatcagag aaatacgcat ttatcgctta tacctcatgg
     1261 tagagaccgt ctggccttct cgtaccacat cgaaccaaat ttccttggcc tttccatcgg
     1321 taaggcgagc gtaaaaatcc ttcaatccag taatatcgcg gtcattaacc ttaacgataa
     1381 catctcccga ttgaagcccc atcacagcgg ccgggctctt gggttgaaca ccactgacaa
     1441 cgacaccgga tacgctttcg gaaatcttaa gtctgtctcg aacttcttta ttaagcggaa
     1501 tgggcataaa gccaggccac agttttgatg cgtcggtaac cgtttgctca ttgcgctctt
     1561 caataagcac cgttacgttt tgctctttac cctgccgcaa tacgcgaaag gttgccttgg
     1621 agccactttc cagatcgcca acgtccctga ccagttgatc taccgctttt acttccttgc
     1681 cattaagggc aatgactacg tcgcccggaa gaatgccgcc tttatctgcc ggagagccga
     1741 taaacacctg ggaggcaagc gcgccatttt tatttttaag gccaagtgcc tcaaagatgt
     1801 ccttatccgc gtttaaaaga gaaacaccaa gccatccata gcgaatcttt ccggttgata
     1861 taaaatcatc tatcgctttt tttacgttat ttatgggtat ggaaaaaccc agtccttgag
     1921 atccgcctgt ggaagaggcg atccacgcat taatacctac aacctgacca tagatattaa
     1981 caagagggcc tcctgaatta ccctgattga tcgctgcatc cgtctgaata aagtcgttga
     2041 tatttccgtt aggaccaccg gagcgaccaa cagcgctcac aataccttgg gtaaccgagg
     2101 aaatataccc aagtggagag cccacggcaa atacgatatc tcccgtttga acggaatcag
     2161 agtcacccat gctggcaacc ggtatggtcg aatcatt
//
LOCUS       DAOQXQ010000278         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56829_length_2195_cov_2.753271, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000278 DAOQXQ010000000
VERSION     DAOQXQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56829_length_2195_cov_2.753271"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="PKH81_05320"
     CDS             complement(<1..1679)
                     /locus_tag="PKH81_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNQ97491.1"
                     /translation="MKNLISTKTARAGIPVLFCFLLFGLPAFTQNPSTNALPVERYAL
                     YVAANDGGAEREKLRYAVSDAERMARTLTEIGGVTGENSLILTDPGRAAIERAFKNFA
                     RQIEGNAGRAKRTEFIFYYSGHSDETALLLGRESLSYAELKKQLAIVPTDVHVVMLDS
                     GYSGEFIRTKGGQRQKPFLVDDSSVVQGHAYLSSSSAFESSQESDAIQASYFTHSLVT
                     SLRGAADTSGDQKVSLNELYHYAFNDTLSRTEKSHLGPQHPSFNITLVGSGDLVLTDI
                     SEADSVLIFPNKAEGRYFIRTQEGVLVSEINKVAGTELALALGQGTYAVTMLSAKDTS
                     VATVILSSGDRLTLSDGQFTPIARSMGRARGDAEDTVKDDSQEPVAPYASWVFGFVPG
                     VTVPRNPGGNAKMSLNALVGKNNRINGVQANGFMGIITQDLHGAQVSGFLSTANGSME
                     GVQASGFMNIADGPIKGAQLSGFGNIVSAGIEGFQGSGFFNVAGGTVEGVQATAFINI
                     AHKVDGAQLSGTINVAREVDGIQIGVVNVARNNSGLALGLLNFIQDGVLDVG"
     gene            complement(1690..>2195)
                     /locus_tag="PKH81_05325"
     CDS             complement(1690..>2195)
                     /locus_tag="PKH81_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97492.1"
                     /translation="NPELLLAQAASGERSKGASMAKDTQLALGKGELYAYRKTGNAVD
                     RLEKGAKVNENDVIQISYFAGSDRFGVILSVDGNGIITRHYPDGGPDAGILKASGEIP
                     LDFSYKLDNAPSFERFILISAPAVFTVQPVISAIEKASRDESFTTMDLSKVLPQSLNS
                     SDIVLIK"
BASE COUNT          507 a          561 c          598 g          529 t
ORIGIN      
        1 ccaacatcca acacgccgtc ctggataaag ttaagaagac ccagggcgag cccggaattg
       61 tttcgggcaa cgttgactac accaatctgg atcccgtcca cttctctggc aacgttgatt
      121 gttccggata gctgtgctcc atcaaccttg tgtgcaatgt tgataaatgc ggttgcctga
      181 acgccctcta ccgtaccgcc tgctacgtta aaaaagccgg aaccctgaaa tccttctata
      241 ccggcggaga cgatgttgcc aaaaccggaa agctgggctc ccttaatcgg gccgtcggca
      301 atgttcataa agcctgaagc ctgtactcct tccatggagc cgttagcggt actaaggaat
      361 cctgaaacct gggcaccatg aaggtcctgg gtaatgatac ccataaaccc gttagcctgc
      421 actccgttga tgcgattgtt tttaccaaca agcgcgttaa gcgacatttt ggcgtttcca
      481 ccgggattac gcggcacggt tacgccgggg acaaagccga aaacccaaga ggcatagggt
      541 gcgacgggtt cttgactatc atctttgact gtatcctctg cgtctccgcg agctcttccc
      601 atgcttcggg caatcggcgt gaactgtccg tcggacaggg taagtctgtc gcctgaagaa
      661 aggataacag ttgcgacgga ggtatccttt gcgcttagca tcgtaacagc ataggtgccc
      721 tgtccaaggg caagggcaag ttctgtgccc gcgaccttgt taatctcgga aacaaggacg
      781 ccttcctggg tgcggataaa atagcggccc tctgccttgt tcgggaatat cagcaccgaa
      841 tctgcttcgg aaatatcggt cagtaccagg tctccggaac ccaccagagt aatattaaaa
      901 gaagggtgct ggggtccaag gtggcttttt tccgttctgg aaagggtgtc gttaaaggca
      961 tagtgataga gctcgttaag agaaaccttt tgatcgccgg aggtatcggc agcgccccgc
     1021 aagctggtaa ccagggagtg ggtaaaatac gaagcttgaa tcgcatctga ttcctgggaa
     1081 gattcaaagg ccgagctgga agacaggtat gcgtgtccct gcacgacgct cgagtcgtct
     1141 accaaaaaag gcttttgccg ctggccgccc ttggtgcgga taaactctcc ggaatagcct
     1201 gagtcaagca taacaacatg aacgtctgtg ggcacaatag cgagttgttt tttcagctcg
     1261 gcataggaaa ggctctccct gcccagaagc aaggctgtct cgtctgaatg gccggagtag
     1321 taaaaaatga actcggtgcg ttttgccctt cctgcgtttc cttcaatttg cctggcaaag
     1381 ttcttgaagg cccgttcaat agccgcgcga ccgggatccg taagaattaa actgttttcg
     1441 ccggtaacac cgccaatctc ggtaagggtg cgcgccatgc gttccgcgtc ggatactgca
     1501 tagcgaagtt tttctcgttc ggccccgcca tcgtttgccg cgacatacag ggcatagcgc
     1561 tctacgggca gggcgttggt agagggattt tgtgtaaagg cgggaagccc aaagagcaaa
     1621 aagcagaaca agactggtat cccggctctt gcggtcttgg ttgatatgag gttcttcata
     1681 acgtactcct tattttatca gtacgatgtc gctcgaatta agggactggg gaaggacctt
     1741 ggaaagatcc atggtggtaa aagactcgtc ccgcgaggct ttttctatag ccgaaatgac
     1801 cggctgcaca gtaaataccg ccggcgcgct tatcaggata aagcgttcaa aggatggcgc
     1861 gttatcgagc ttgtatgaaa agtccagggg gatttcgcct gaagctttaa gaatgcctgc
     1921 atcgggtccg ccgtcgggat aatggcgggt aataatcccg tttccgtcta ccgaaaggat
     1981 aacgccgaag cgatctgagc cggcaaagta gcttatctgt atcacgtcgt tttcgttaac
     2041 cttggcaccc ttttccagcc ggtctaccgc gttaccggtt ttacggtatg cgtagagctc
     2101 tccctttcca agggccagct gggtgtcttt ggccatgctt gcgccttttg atcgctcacc
     2161 gcttgccgcc tgggcgagca gtaattcggg gtttc
//
LOCUS       DAOQXQ010000279         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_56955_length_2192_cov_3.503042, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000279 DAOQXQ010000000
VERSION     DAOQXQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56955_length_2192_cov_3.503042"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKH81_05330"
     CDS             complement(<1..186)
                     /locus_tag="PKH81_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97493.1"
                     /translation="MKRYQVLIVILLFASFSLSAQVACDPEDFLYQDISIWQTMGIIR
                     PLPAARPYPLQLIKGILS"
     gene            complement(261..1412)
                     /locus_tag="PKH81_05335"
     CDS             complement(261..1412)
                     /locus_tag="PKH81_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ97494.1"
                     /translation="MKQRIAFLYLNTGGGHVAPARALSSGLAERYPETDETHLFNGFS
                     QKMRVCRFFFEDGYQISSNYFEASYVLFYRMTEIPQAIRFGNYFVSIHGEHHLARFFR
                     EKQITKVVCLHEILLIIARKALARVNPDASFITLVTDPFTAHGLWFYVKDTELVVFSQ
                     KLRKEAIEKHGFVPQRVHTFPFILNPAFDRVYTDEEKRAVRSRLGIAEDQKVLLIAGG
                     GEGLKSADRIVAHYLRDKSYRSRNEVLLVVCGRNRLLKMQVQFMADRSRSKAVRVFGF
                     VSFMPDLLNIADCVITKGGASTVMEVLAVGKPVIFSTFIRGQELGNVLYVVTNGAGWY
                     LKKPKEILGQARAVLDNPELATRIRQNIARLGVRNGLYDVMRFIHEWKQ"
     gene            1518..>2192
                     /locus_tag="PKH81_05340"
     CDS             1518..>2192
                     /locus_tag="PKH81_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ97495.1"
                     /translation="MNIALFSDSYIPTKSGVVTVILQLRKALESMGHHVVIVTVETTP
                     GERKTREDDPCIFRARSIPLGLGTDQFIGFPQKRKIMRFLREHQIEIIHSHTEFYIAH
                     AAKSLGRALHIPTIATTHTMWEDFYDYYVPMARLIPVKAIRKIVKRLYKKFYAFINVS
                     SKAREYFKREHMLPHIPSAVIPNAVDTEAFNGNSDSTDALQAMRKNWGISETETVLLF
                     VGRIGEE"
BASE COUNT          633 a          522 c          507 g          530 t
ORIGIN      
        1 ggagaggatg cccttaataa gttgaagggg atatggcctg gcagcaggca gggggcgaat
       61 gatacccatg gtttgccata tgcttatatc ctgatagaga aaatcttccg gatcgcaggc
      121 tacctgggca gacagggaaa aggaggcaaa aaggagaata acgatgagaa cctgatatcg
      181 cttcatgtaa accatcctgc gctggggtgt aaaaaagtta tgttactata accgagttta
      241 gttctatcgc tcaagagcct ttattgcttc cattcatgga taaagcgcat cacgtcatac
      301 agtccgtttc ttactcccag acgggcaata ttctggcgaa tgcgcgttgc aagttccgga
      361 ttatcaagca cagctcgtgc ttggccaaga atttctttgg gttttttaag ataccagcct
      421 gccccattgg taactacata caggacgttt cccagctcct gtccccggat aaaggtggaa
      481 aaaataaccg gtttgccaac cgcaagaacc tccatcaccg tggatgctcc tcccttggta
      541 atgacacaat cggcgatatt cagtaaatcg ggcataaagg aaacaaagcc aaaaacccgt
      601 accgctttgg aacgggaacg gtcggccata aactgaacct gcatcttgag taagcgattt
      661 cgtccacaca ccactaacag aacctcgttt cgcgaacgat aggatttgtc cctgagatag
      721 tgggcaacaa tacgatcggc gcttttaaga ccttcccccc ctccggcaat aaggagtacc
      781 ttttgatctt ctgctatacc cagacgcgaa cgaaccgctc ttttttcctc gtcggtatat
      841 actcgatcaa aggcaggatt aagtataaag ggaaaggtat gcacccgctg tggtacaaag
      901 ccatgttttt ctatggcctc cttcctgagt ttctgcgaaa aaacaaccag ttcggtatcc
      961 tttacataaa accaaaggcc atgggcggta aaggggtccg ttaccaacgt gataaaagat
     1021 gcgtcgggat taacccgcgc aagggcctta cgcgcgatta tcaacagaat ctcatgaaga
     1081 cagacaacct tggtaatctg cttttcccta aagaagcggg ccaggtgatg ttctccatga
     1141 attgatacga aataattgcc aaaccgtatt gcctgtggta tttcggtcat ccggtaaaaa
     1201 aggacatagg atgcttcaaa ataattagag gatatctgat atccatcttc aaaaaagaag
     1261 cgacacaccc gcatcttctg ggaaaaaccg ttaaaaagat gggtttcgtc ggtttctgga
     1321 tagcgctcgg caagaccgct cgaaagggcc cgcgcagggg ctacatggcc gccgccggta
     1381 ttaaggtaca gaaaggctat acgttgcttc atatatacac atgagtataa ccggaggatg
     1441 acggggaaac aagaaagatt ttttcgatta tccaaaacga gtttacaaag gactatggcg
     1501 cagatatact ctttttcatg aatatagccc tgttttcaga cagctacatc ccgactaaaa
     1561 gtggggttgt taccgtcata ctccaactcc gcaaagccct ggaatccatg ggccaccatg
     1621 tggtaatcgt tacagtggaa acgacccctg gagagcgaaa aacgagggaa gatgatccgt
     1681 gtatcttccg tgcacggtct atacccctgg gactggggac cgatcaattt atcggctttc
     1741 cgcaaaagcg taaaatcatg cgctttcttc gcgaacatca gatcgaaatt atccatagcc
     1801 ataccgagtt ttatattgcc catgcggcaa aaagtcttgg ccgtgccctc catattccaa
     1861 ccatagcgac aacccatacc atgtgggaag acttttacga ctactatgtg cccatggctc
     1921 gccttatccc ggtaaaagcc atccgtaaaa tcgtaaaacg cttgtacaag aaattttatg
     1981 cctttatcaa tgtctccagt aaggctcgcg aatactttaa acgggaacat atgcttcccc
     2041 atatcccttc ggcagtaatt cccaatgcag tggatacaga ggcctttaac ggtaactctg
     2101 attcaacaga cgccttgcag gcaatgagga agaattgggg gattagcgaa acagaaacgg
     2161 tactgctgtt cgttggcaga atcggggaag aa
//
LOCUS       DAOQXQ010000280         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57009_length_2191_cov_2.640449, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000280 DAOQXQ010000000
VERSION     DAOQXQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57009_length_2191_cov_2.640449"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..678
                     /gene="ruvA"
                     /locus_tag="PKH81_05345"
     CDS             64..678
                     /gene="ruvA"
                     /locus_tag="PKH81_05345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ97496.1"
                     /translation="MFNSISGTITAKLPNSLFIENSGIEWDFSVPSLSLDSFGHLGEI
                     ARVYVWLYHREDQMRLFGFPTPQERDVFLELTKVDGIGPRQAIRILSSISSSDLEGAL
                     EGEDVDRLSAAPGIGKKTAQKMILSLKGKLAHGKEGQMPSDGGEHADIIGALVEMGFD
                     RKASASQVSNIALELEKQSGGHTSSTKAEREQEIFRRAIVQLSS"
     gene            690..1736
                     /gene="ruvB"
                     /locus_tag="PKH81_05350"
     CDS             690..1736
                     /gene="ruvB"
                     /locus_tag="PKH81_05350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNQ97497.1"
                     /translation="MKSADESLVRPEARLDDERDRALRPRLLADFQGQEQIKTNLAVF
                     VEAARCRGESLDHLFLIGPPGLGKTTLAQITANELGVDFKVTSAPALDKPKDLAGILT
                     TVTERTVFFIDEIHRLKPAIEEMLYIAMEDYELDWIIGQGPSARTVRIPIPPFTLIGA
                     TTRAGMVSSPLISRFGIVQRFAFYTEDELASIIARSASILNVRITDRAAHALASCSRG
                     TPRVANRLLRRMRDFAEVLEDGTVTDEVVRQGLLRLEIDSLGLERYDREILTSIMDNY
                     SGGPVGAETLAISVGESQETLEDYYEPYLIQAGLIQRTPRGRMVTDRAYTHLGRQRKS
                     GNKDRSDENERLPF"
     gene            1711..>2191
                     /locus_tag="PKH81_05355"
     CDS             1711..>2191
                     /locus_tag="PKH81_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNQ97498.1"
                     /translation="MKTSDFHFDIPEDLVAQQPCEKRGEDRLLVLQRSSGILSDHMFS
                     DFPSLIEKGSLLVFNDSRVRRSRIFATVEASGNRAEFLLLEELSQEGFLAGSLWKVMA
                     KNAKRQKAGRRYLFDDGRSARIVGADECPVAGELEGSEFRILAFDEPINESWLETNGH
                     "
BASE COUNT          498 a          554 c          577 g          562 t
ORIGIN      
        1 ataccgccga tctttgtggc tctattagtt gtcaattgcc gcaaaaaaag agataatgct
       61 cgtatgttta atagcatttc cggcactatt accgctaaat tacccaattc cctttttata
      121 gaaaactcag gtattgagtg ggacttctcc gttccctccc tttcccttga ttcctttggt
      181 catcttggcg aaatcgctcg tgtatatgtg tggctctatc atcgcgagga tcaaatgcgt
      241 ctgtttggtt ttcctactcc ccaggagcgg gatgtctttc ttgagttaac caaggtggat
      301 ggcattgggc cccggcaagc tattcgtatt ctttccagca tctcttcttc cgatctggaa
      361 ggagcccttg aaggcgaaga cgttgaccgg ctttctgccg ctcccggcat cggaaaaaag
      421 actgcgcaaa agatgatttt atcgctaaag ggcaaacttg ctcacggcaa ggaaggccag
      481 atgccttctg atggaggcga acatgccgac attatcggcg cccttgtaga gatgggtttt
      541 gatcgtaagg cttctgcctc tcaggtgtcc aacattgccc tggagcttga aaaacaatca
      601 ggcggacata cgtcttccac aaaggccgaa cgcgagcaag aaattttccg tcgcgccatc
      661 gttcagttaa gttcataggg gagtgggata tgaaaagcgc cgacgaatcg cttgtcagac
      721 cggaagctcg tcttgacgat gagcgggacc gggctttacg tccccgtctt cttgcggatt
      781 ttcaggggca agagcagatc aagactaatc ttgcagtgtt tgtggaagct gcccgctgcc
      841 gcggagaaag ccttgatcat ctttttttga tcgggccgcc cggccttggc aaaaccacgc
      901 ttgcccaaat taccgcaaac gagcttggtg tagattttaa ggtaaccagc gctccggccc
      961 tggataagcc aaaggatctt gccggcatcc ttactacggt aaccgagcgc acggtgtttt
     1021 ttatcgacga gattcatcgg ctcaaacccg ccatcgaaga gatgctctat attgccatgg
     1081 aagattacga actggactgg attattggac agggtccaag tgcccgtacg gttcgcatcc
     1141 caattccgcc ttttacgctt ataggggcta ctacccgcgc gggtatggtt tcaagcccgc
     1201 ttattagccg ctttggcatt gttcagcgtt tcgcttttta taccgaggat gaactcgctt
     1261 ctataatcgc gcgctctgct tccatcctta acgttcgcat taccgacagg gcggctcatg
     1321 cactggcatc ctgttcacgc ggcacaccgc gcgtggctaa tcgcctgctt cgccgtatgc
     1381 gcgactttgc cgaggtactg gaagacggca ccgttaccga cgaggttgtc cgtcagggct
     1441 tgttacgcct ggagatagac tcgcttggcc ttgagcgcta cgaccgcgag atacttactt
     1501 ccattatgga caattactca ggcgggcccg tcggagcgga aaccctcgcc atatccgtcg
     1561 gtgaatccca ggaaaccctg gaagattatt acgagcccta tttgattcaa gcaggtctta
     1621 tccagcgcac tccccggggc aggatggtaa ccgatcgcgc gtatacccat ttgggtcggc
     1681 aaagaaaatc aggcaacaag gatcgaagcg atgaaaacga gcgacttcca ttttgatatt
     1741 cccgaggatt tagtagcgca acagccgtgc gaaaagcgcg gagaggaccg gcttttggtg
     1801 ctgcaacgct cgagcggtat tctgagcgat catatgtttt cagattttcc ctcccttata
     1861 gaaaagggga gtcttttggt atttaacgac tcacgcgttc gtcgttcccg catttttgct
     1921 actgtagagg caagcggaaa ccgcgcagag tttttacttt tggaagagct gtctcaggaa
     1981 ggttttttag ccggaagcct ttggaaggtc atggcaaaaa acgccaagcg gcaaaaggcg
     2041 ggtcgccggt atctctttga tgacggtcgt tctgctcgga ttgtgggcgc agacgaatgt
     2101 cctgtggcgg gggagttgga aggaagtgag tttagaatcc ttgcctttga cgagcctata
     2161 aacgaaagct ggcttgaaac caacggacac t
//
LOCUS       DAOQXQ010000281         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57020_length_2191_cov_1.946629, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000281 DAOQXQ010000000
VERSION     DAOQXQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57020_length_2191_cov_1.946629"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKH81_05360"
     CDS             <1..763
                     /locus_tag="PKH81_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97499.1"
                     /translation="VSAAYTGLLNVRKTGAMMSGDDYADSITDDVYAFGAKRGVGKFT
                     IHLPESLGPVDIIMEGLGQYDLRDQVDGDSEELVHTGYGTLMLTGPITGTVFFTVSGT
                     FQTGVLEAEEDYSENAMLARARIDAFPMPLHHLYGEVLYTTAEGDFFTTFLPITVQTP
                     GTLYGSGYSNIIKGSLGWGFNPRNFLNFDLSGSVFMYPDEMEGSDGLYSATELNAGLT
                     YKATSDLNLRLNGALLFPKDEDMQYQAELKVVFGL"
     gene            802..1494
                     /locus_tag="PKH81_05365"
     CDS             802..1494
                     /locus_tag="PKH81_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNQ97500.1"
                     /translation="MKRILITAMVLTIAIVSSQGLFALQGEVVTVNGKVEIKSTSGTW
                     IPVKAGQKISEGTMISTGFKSEATVKLGASILTVKPLTRMTLNQLVEREDTVDTELYL
                     EVGNVKAEVNSLNNKRNGFTVKSPVATASVRGTIFEMGDKLVVLQGAVLYVSAIGQSR
                     MGTAGQTMETMNETIASPVAVMMAEMNPIKLSGLPSAENLSPILAAISPPQMRPVIRD
                     INQETSLVLTIQ"
     gene            complement(1555..>2191)
                     /locus_tag="PKH81_05370"
     CDS             complement(1555..>2191)
                     /locus_tag="PKH81_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668208.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNQ97501.1"
                     /translation="VKEAVTRDTPLRYDDSTGYLGTAVSAGNEILRVTPYDRVLVMRK
                     TGMYAVMDVPERIFVDKGMLYCGFSDKETLAKILFTLIYRDPKTGYPYIKRCRIGGCI
                     LNRDYFLVPAGAEVLYAGTREKASVTVYYEPKARLKTKEQTFKIHDYPEKGLKAQGVR
                     LAPRAAASAREEELVAGGLMKKAQERKVTDKKAPAKKTPAVKTPAKKTKKK"
BASE COUNT          594 a          519 c          516 g          562 t
ORIGIN      
        1 tgtatcggcc gcttatacag gattactcaa cgttcgaaaa acgggagcca tgatgagtgg
       61 cgacgactat gcagactcca taacggatga tgtatatgcc tttggcgcca agcgaggcgt
      121 tggaaagttt accatccatc ttcccgaaag cttgggcccg gtagatatca ttatggaagg
      181 ccttggccaa tatgatcttc gcgaccaggt agacggcgac agtgaagaat tggtacatac
      241 cgggtatggt accctgatgc ttaccggtcc gataaccggt accgttttct ttaccgtttc
      301 cggaacattc caaaccggcg tactggaagc cgaagaagat tattcagaaa acgccatgct
      361 tgcccgtgcc agaattgacg cctttccaat gccgctccat catctgtatg gagaagtgct
      421 gtacacaaca gcagagggtg acttctttac cacctttttg ccaatcactg ttcaaacccc
      481 gggaaccctc tatggctcag gatacagcaa cattatcaag ggctcacttg gatggggctt
      541 taatccaaga aactttctta actttgacct ttccggctct gtatttatgt atcccgatga
      601 gatggaaggt tcagacggtt tgtatagtgc taccgaattg aatgcaggac ttacctacaa
      661 agcaaccagc gatttgaacc tcagattgaa tggcgctttg ttgttcccca aagacgaaga
      721 tatgcaatac caggccgaac tcaaggtagt attcggactg taactagttg ttcacgatag
      781 agataacata ggaggtttga tatgaagcga atcctgatca ctgcaatggt attgaccatc
      841 gcaattgtca gctcacaggg actttttgcc ctgcaaggcg aagtggtaac cgtaaacggc
      901 aaggtagaaa taaagagcac ctccggaacc tggattccgg ttaaagctgg acaaaaaata
      961 tctgagggaa caatgatctc gaccggcttt aagagcgaag ctaccgtaaa actcggagcg
     1021 tctatcctga cagtcaaacc gcttacccgt atgacactta atcaactcgt agagcgcgaa
     1081 gacacagtag ataccgaatt gtatctggaa gtagggaacg taaaggcaga ggttaactcc
     1141 ctgaataaca agcgaaatgg ctttaccgta aaaagccctg ttgctaccgc gtcggttcgg
     1201 ggaacaatat ttgaaatggg cgacaaactg gtggtgctac agggagcagt tttatatgta
     1261 agcgctatcg gacaatctcg tatgggaaca gccggacaaa cgatggaaac aatgaacgaa
     1321 accattgcct ctcctgtggc ggttatgatg gctgagatga acccgattaa actctctggt
     1381 ttaccgagtg cagaaaacct ctctcccata ttggctgcca tctcgccgcc ccaaatgaga
     1441 ccggttatta gagacataaa ccaggaaaca tctttggtac taactattca gtaaaaagga
     1501 ttttgtaaca ggttaagaaa aagcgcgcag cccgcagggg atacgcgctt ttttttattt
     1561 ctttttggtc tttttcgcgg gagttttgac tgccggagtt tttttggctg gagccttttt
     1621 atccgttacc tttcgctctt gtgccttttt catcaagcca ccagcaacta actcttcctc
     1681 gcgagcagaa gcggcagcgc gaggggcaag gcgaacacct tgagccttaa gccccttctc
     1741 gggatagtca tgaattttga atgtttgttc ctttgttttt aagcgagcct ttggttcata
     1801 atataccgta accgaggctt tttccctggt tccggcatat aagacctcgg ctccagcggg
     1861 taccaggaag taatccctat taagaataca ccccccgata cgacatcgct taatatacgg
     1921 gtagccggtt tttggatcgc gataaatgag ggtgaatagt attttggcaa gagtttcctt
     1981 atccgaaaaa ccgcaataca gcatgccctt atccacaaaa attctttccg gaacatccat
     2041 tactgcatac atacccgttt ttctcattac caggacacgg tcataggggg taacacgcaa
     2101 gatttcatta ccggcggaaa cagccgttcc aagatagcca gtcgagtcgt catacctgag
     2161 cggcgtatcc cgggtgaccg cttctttcac g
//
LOCUS       DAOQXQ010000282         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57069_length_2189_cov_3.226336, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000282 DAOQXQ010000000
VERSION     DAOQXQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57069_length_2189_cov_3.226336"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2189)
                     /gene="rpoC"
                     /locus_tag="PKH81_05375"
     CDS             complement(<1..>2189)
                     /gene="rpoC"
                     /locus_tag="PKH81_05375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNQ97502.1"
                     /translation="QVAALRSILYYEKFIVIDAGDTDLKKNQLLTEEEYQEATERYGG
                     SFTAGMGAEAIKTLLENLNLDELAAELRAKMIEKGAKSDKRLLKRIEIVENFRASTNK
                     PSWMVLDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLMGLNAPDII
                     IRNEKRMLQEAVDALFDNSKRKRVIKGASNRPLKSISDMLKGKQGRFRQNLLGKRVDY
                     SGRSVIVVGPELKLWQCGLPTKMALELFKPFIMKKLVDKDVVYNIKKAKLLVEQESPE
                     VFVVLDEVVREHPVMLNRAPTLHRLGIQAFEPVLVDGKAIRLHPLVCKAFNADFDGDQ
                     MAVHVPLTQAAQMECWTLMLSSRNLLDPANGKSIVYPSQDMVLGLYYLTKIKPNGKGT
                     GHRFTNVEEVMMAAEIGSIGWQSLINVNYRGALVETTAGRLIFNEEMPEGVPFTNLTL
                     DDKQIRKLIESVHKASGPWLTVRMLDQLKDTGYKYATFFGATLSMDDIIVPDEKQQMI
                     EKANKEVEGIQKQYQQGHITQDERYNRVVEVWSKTNEDLTNIMMKTLEGDKDGFNTIY
                     MMATSGARGSRNQIRQLAGMRGLMAKPNGDIIELPIRSNFKEGLTVIEFFISTNGARK
                     GLADTALKTADAGYLTRRLVDIAQDVVVNEQDCGTINGTEYQAIKEGDDIVESLSSRI
                     TGRYTLERVVHPITHDVIIDVNEFITDEIAQDIEAAGVESVKLRTVLT"
BASE COUNT          559 a          541 c          518 g          571 t
ORIGIN      
        1 gtaagtacgg tcctgagctt aaccgattca acaccggcag cttcaatatc ctgagcaatt
       61 tcgtcggtga taaattcgtt aacgtctata ataacgtcat gggtaatagg atgaactacg
      121 cgctccaggg tgtaacgtcc ggtaatgcgg ctggagaggg attcaacgat gtcgtcacct
      181 tccttgatag cctgatactc ggtaccgtta atggttccac aatcttgttc gttaacaacc
      241 acatcctgcg caatatcgac gagacgacga gtaagatatc cggcgtctgc ggtcttaagc
      301 gcggtgtcgg ccagaccctt acgggcaccg ttggtcgaaa taaagaattc gataacggta
      361 agaccttcct taaagttaga gcggatcgga agttcgatga tgtcgccgtt tggcttggcc
      421 ataagaccac gcataccagc gagctgacga atctggttgc ggctaccgcg cgcaccagaa
      481 gtagccatca tatagatggt attaaatccg tctttatcac cctcaagggt cttcatcatt
      541 atattagtaa gatcttcgtt ggtctttgac caaacctcaa ctacgcggtt atagcgttcg
      601 tcctgcgtaa tatgaccttg ctgatactgc ttctgaatac cctctacttc cttattggcc
      661 ttctcgatca tctgctgctt ttcgtccgga acgataatgt cgtccataga aagggttgcg
      721 ccaaagaagg tagcatactt atagccggta tctttgagct gatcgagcat acgaacagtt
      781 agccagggac cacttgcctt atgaacagat tcaataagct tacgtatctg cttgtcgtcc
      841 agggtaagat tagtaaaggg aacaccttcg ggcatttctt cgttgaatat taaacgaccg
      901 gcggtggttt ccaccaaggc gccccggtag ttaacattga taagcgactg ccagccaata
      961 gagcctattt cggcagccat cattacttct tcaacattgg taaagcgatg accggtaccc
     1021 tttccgtttg gttttatctt ggtaagataa tacaaaccca aaaccatatc ctgagaagga
     1081 tatacaatgg actttccgtt tgcagggtca aggaggtttc ggctggagag catgagcgtc
     1141 cagcattcca tctgagctgc ctgggtaagc ggaacatgga ccgccatctg gtctccgtcg
     1201 aagtcggcgt taaacgcctt acataccagg ggatgaaggc gaatagcctt accatctacc
     1261 agcaccggct caaaggcctg aataccaaga cggtgaagag tcggcgcgcg gttaagcata
     1321 actggatgct cgcgtacgac ctcgtccaga acgacaaaca cctccgggga ttcttgctcc
     1381 accaaaagct tggccttctt gatattgtat acaacatctt tatcaacgag tttcttcatg
     1441 ataaatggct tgaaaagttc aagagccatt ttggtgggaa gaccgcattg ccagagctta
     1501 agctcgggac caactacgat taccgaacga ccggagtagt caacgcgctt tccaagcagg
     1561 ttctggcgga atcgcccctg cttaccctta agcatatccg aaatagactt gagcggacga
     1621 ttggacgctc ccttaataac gcgctttcgc tttgaattat caaagagggc atctaccgct
     1681 tcctgaagca tacgcttttc gttccggata atgatatcgg gagcattaag ccccataagc
     1741 cgcttaagac ggttgttacg gttaataaca cggcgataca agtcgttaag atcgctggtt
     1801 gcaaaacggc caccgtcgag ctggaccatc ggcctgagct cgggaggaat aaccggaata
     1861 acatcaagaa ccatccagga aggcttgttg gttgacgcgc ggaaattttc tactatttcg
     1921 attcttttaa gaagccgttt atcgctcttg gcacccttct cgatcatctt ggcacgaagt
     1981 tccgcggcaa gttcatcaag gttcagattc tcaagaagcg ttttaatcgc ttcggctccc
     2041 ataccggcgg taaaagatcc accgtagcgc tcggttgctt cctggtattc ttcttcagtg
     2101 agcagctggt tcttcttaag atctgtgtcg cccgcatcaa ttacaataaa cttttcgtag
     2161 tacagaattg atctgagcgc ggcaacttg
//
LOCUS       DAOQXQ010000283         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57120_length_2188_cov_2.639475, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000283 DAOQXQ010000000
VERSION     DAOQXQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57120_length_2188_cov_2.639475"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKH81_05380"
     CDS             complement(<1..711)
                     /locus_tag="PKH81_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ97503.1"
                     /translation="MSQAIAVIGAGAWGTAMAISLAKKGHEVTLWALEQDVVASINER
                     HENTRFLPGFPTPDNLVASADIRAVATGKSFLILASPSLYLARTMQGLLDVPNIKDGS
                     TCIGVLTKGFIPSKDGPRLIVETLESVLPESYRKSLVYIAGPSHAEEVAMGKVTGLIA
                     ASENPMASIRFREVLKSKGLLVYSSLDVIGVQICAAAKNVVAVAFGILDALAEKSEAF
                     GDNTESLLLAAGLNEIQII"
     gene            complement(724..1173)
                     /gene="ndk"
                     /locus_tag="PKH81_05385"
     CDS             complement(724..1173)
                     /gene="ndk"
                     /locus_tag="PKH81_05385"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ97504.1"
                     /translation="MAIQRTFAMLKPGVLQRRIVGDVLARFERKGLHIVGLKILTIPP
                     LMAATHYAEHKGKSFYDDLISYIISGPVVAMAIEGDEAIQLVRRLCGPTAVCESLPGT
                     IRGDYCLHTNHNIIHSSDSPESADRELKLFFQPNEIVSWDDGNKAWF"
     gene            complement(1209..2048)
                     /locus_tag="PKH81_05390"
     CDS             complement(1209..2048)
                     /locus_tag="PKH81_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ97505.1"
                     /translation="MIRETKGTAIASLVLCALLWSSAGVLVKLVSWDSFAIAGIRSLV
                     GLLTMSLFLGLPRLTFDRNQLFAAFFYSLTMILFVYANKMTSAANAVLLQYTSPVYLI
                     ILGRWLLSSEKAMLADWVTVVGVFCGMILFFLDDISLGSNIGNVLAVLSGVSFALTMI
                     FMRRQKDGRPADSFMLAHALTFVVSLPFIIRGGVPSSISILGLVLLGVFQIGFSSILF
                     GRGIRGVTAIESSIITMIEPAFNPVWVLIFIGERPSLRSLAGGAIILVCVTGRTVLKS
                     RKI"
BASE COUNT          640 a          542 c          500 g          506 t
ORIGIN      
        1 aatgatctgt atttcattca agccggccgc taaaagaaga ctttcggtat tatcgccaaa
       61 ggcttcggac ttctcggcaa gggcatccaa tatgccaaag gcgacggcga caacattttt
      121 tgcggctgcg caaatctgta cgccgataac gtcaagactc gaatacacca acaggccttt
      181 tgatttaaga acctccctaa atcgaattga tgccataggg ttttcgctgg cggcaataag
      241 cccggtaacc ttacccatcg caacttcttc tgcatgactt ggaccggcaa tatacacaag
      301 ggacttgcgg tacgattcgg gcaagaccga ttccagggtt tctacaataa ggcgaggtcc
      361 atccttggag ggaataaagc ccttggtaag tacaccaata caggttgagc catctttaat
      421 attgggaaca tctaaaagac cttgcattgt cctggcaaga tagagagaag ggcttgccaa
      481 tattaaaaag gacttaccgg tagcaaccgc gcggatatcc gcgctggcga ccagattgtc
      541 gggagtaggg aacccgggta aaaaccgggt attttcatga cgctcattta tcgaagcgac
      601 tacgtcctgt tcaagagccc ataaggttac ttcatggccc tttttggcca gagaaattgc
      661 cattgcagtt ccccaggcgc cagctccaat cactgcgatt gcttgactca tgatgttcct
      721 ccgttaaaac caggctttgt tcccgtcgtc ccaggatacg atctcgttgg gttggaaaaa
      781 gagtttaagc tctctatccg cgctttccgg cgaatccgag gaatggatga tgttatgatt
      841 ggtatgaaga cagtaatccc cgcggatggt tcccggcagg gattcgcaca cagccgtagg
      901 tccacataaa cggcgtacca attgtattgc ttcatcaccc tctatggcca tggctacaac
      961 cgggcccgag ataatgtatg aaatcaagtc atcataaaac gacttaccct tgtgttctgc
     1021 ataatgagtg gcagccataa ggggcggaat cgtcaaaatc tttaatccga cgatatgtaa
     1081 tccttttcgc tcaaatcggg caaggacgtc gcccacaatc cttcgctgaa gcacacccgg
     1141 cttcagcatg gcaaaggttc tttgaattgc catgatatgt attcctccaa ataataggaa
     1201 aaagtatatt atatttttct gcttttgaga actgtccggc cggttacgca taccaggatg
     1261 atcgcgccac cagccaggga ccgcagagaa ggccgctccc ctataaaaat aagaacccag
     1321 accggattaa acgccggttc aatcatggta ataatagacg attcaatcgc cgttacaccc
     1381 cggatacccc tgccaaatag aattgatgaa aatccaatct gaaacacccc aagcaagacc
     1441 aaaccaagaa tgcttatgga tgaaggaact cctccccgga taataaaggg aagcgacacc
     1501 acaaaggtta gggcatgtgc aagcataaag gaatctgcag gacgcccgtc tttttgtctt
     1561 ctcataaaaa tcatggtaag agcaaaggaa acaccggaaa ggacggctaa cacattccca
     1621 atattactgc caaggctaat atcatctaaa aaaaacagga tcatcccgca aaacacacca
     1681 actacggtaa cccaatcggc aagcattgct ttttcgctgg aaaggagcca tcggccaagg
     1741 atgattagat ataccgggga tgtgtattgc aatagaacgg catttgccgc acttgtcatc
     1801 ttattggcat acacaaagag aatcatcgta aggctataaa aaaaagcggc gaatagttga
     1861 ttacggtcaa aggtaaggcg gggcaagcca agaaagagtg acatcgttag caagcccacc
     1921 aaactccgta tcccggcgat tgcaaaggag tcccaggata cgagcttgac taatactccc
     1981 gcggaactcc acaacagcgc gcacagaaca agggacgcta tcgcagtccc ctttgtttcg
     2041 cgaatcatgt ttgagctttt ttatcttccc gtacccgatt acaggcatag gtaagggcat
     2101 cttcaatcat cgcaggatca ggttcgggaa taaagccctt gtccattaac agtgaactca
     2161 tctctctgga ctggcgtata tcggcaag
//
LOCUS       DAOQXQ010000284         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57124_length_2188_cov_2.500234, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000284 DAOQXQ010000000
VERSION     DAOQXQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57124_length_2188_cov_2.500234"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="hydG"
                     /locus_tag="PKH81_05395"
     CDS             complement(<1..1257)
                     /gene="hydG"
                     /locus_tag="PKH81_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNQ97506.1"
                     /translation="MKDDVSTRKVSVAEWQKNLIKQSEIDPYLEDGKDFIDEEAIETA
                     LEQGKQASPERIRAIIKKAYDIKLMSAEDTAALVNVTDPALKEEVFKAAREIKTKVYD
                     NRVVTFAPLYCGSKCVNSCKYCGFRCENNSIDRRVLSMDEIEAEARVLAAKIGHKRLV
                     VVYGEHPDTDADYIAESMKRIYSIKEKVRGGVGEIRRVNVNAAPLPIEGLKKLHEAGI
                     GTYQVFQETYHKKTYESVHPAQTLKGNYRWRLYALHRAMDAGIDDVAVGALFGLAPWR
                     FEIMGLVAHARDLEKRFGLGPHTVSVPRLEPAADTDFSWVQNWVSDDDFRYLIACIRL
                     AIPYAGLIVTNREKPEMIRSVIKMCTQRDADTRVGIGAYVEAFSAHDQREETQQFILG
                     DTRSLDEIIRELASMGHIVSFCTAGYR"
     gene            complement(1269..1520)
                     /locus_tag="PKH81_05400"
     CDS             complement(1269..1520)
                     /locus_tag="PKH81_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HNQ97507.1"
                     /translation="MENRSVGVVAIIIKNRAESANLVNDVLAPFGDMVLGRMGLPYHE
                     RDLNIITLIVDASTDRIGSLTGKLGQIPGVTVKSTLAKI"
     gene            1808..>2188
                     /locus_tag="PKH81_05405"
     CDS             1808..>2188
                     /locus_tag="PKH81_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97508.1"
                     /translation="MRATRPVFVFAFALSLACMVSPLSAGGKKEEKRSVLIILDGKGF
                     SESILAGTPEYLAQWGLTCDISAPDKENVISDTGRNRKDVRSLTEVSDSDYSALLIVP
                     SASRSSLMEDPEYVEIIGKFYRAKK"
BASE COUNT          538 a          525 c          547 g          578 t
ORIGIN      
        1 gcggtatccc gctgtgcaaa acgagacgat gtgacccatt gatgcgagct cgcgaataat
       61 ctcgtccagg ctgcgtgtgt ctcccaaaat aaactgttgt gtttcttctc tctggtcgtg
      121 tgcagagaag gcttctacat acgcaccgat tccaacccgg gtgtctgcgt cccgctgtgt
      181 acacatcttg atgaccgaac gaatcatctc cggtttttca cgattggtaa caataagacc
      241 cgcataaggg atggctaagc gtatacaagc tatcaggtag cgaaagtcat cgtcgcttac
      301 ccagttctgt acccaggaaa aatcagtgtc tgccgcaggc tcaagccggg gaacagaaac
      361 cgtgtgaggt ccaagaccaa atcgtttttc aaggtctcgt gcatgggcta ccagacccat
      421 tatttcgaat ctccaggggg caaggccaaa aagggctcca acagccacat cgtcgattcc
      481 tgcgtccatt gccctgtgaa gggcgtagag gcgccagcgg taatttccct taagggtttg
      541 tgccggatga accgattcat atgttttctt gtgataggtt tcctggaaaa cttggtaggt
      601 tccgatgccg gcttcatgta gtttttttaa tccctcgatg ggaagtggag cggcatttac
      661 gtttacccgg cggatttctc ccacaccgcc gcggactttt tccttgattg aataaattcg
      721 tttcatgctt tcggcgatgt agtcggcgtc ggtatcgggg tgttctccat atacgacaac
      781 aagacgcttg tgtccaattt tggcggcaag aacacgggct tccgcttcga tctcgtccat
      841 ggacaaaaca cgacgatcga tagaattgtt ttcgcagcga aaaccgcagt acttacagga
      901 gtttacacat ttactgccgc aatataaggg agcaaaggta acaacgcgat tatcatagac
      961 cttggttttt atttcgcgag ctgccttaaa gacttcttcc ttgagcgccg gatcggtaac
     1021 attgaccaga gccgccgtgt cttcggcgct catcaacttg atgtcgtacg ccttcttgat
     1081 aatcgctcta attcgttccg ggcttgcctg ttttccttgc tcaagtgccg tctcgatcgc
     1141 ttcttcgtca ataaaatcct taccgtcttc cagataggga tcgatttcac tctgtttgat
     1201 caagtttttt tgccactcgg caacagatac tttccgggta cttacgtcgt ctttcatgtc
     1261 ttgtctcctt atatttttgc cagggtggac ttaacggtaa cgcctggaat ttgtccgagc
     1321 ttcccggtaa gagagccaat gcggtctgtg gatgcgtcta caatgagggt gatgatgtta
     1381 agatcccgct cgtgatacgg aaggcccatg cggccaagca ccatatcgcc aaagggagcc
     1441 aatacatcgt tgacgaggtt tgcgctttca gcgcgatttt tgatgataat ggcaacaacg
     1501 ccgacagaac ggttttccat ccgtttcctc cttacaagct atacgctgcg gggagggtac
     1561 ggacataacc gattatagcg cgcgaaaaga cgggtaacga gaaggcgcgc aaagagggtt
     1621 aggtgccggc ccttggcttc agagtgcatg gttggcgatg gccgcctatg catatcctga
     1681 ccgcaggtag gtatatagtt ataatatgct atcgataaaa aaatagcaat aagggaagat
     1741 aaagcatttg acaaagtgta cgaccctgac tacccttgag caacaacaag aaaaaaagag
     1801 gtccaagatg cgcgctacac gcccggtttt tgtcttcgcg tttgcactga gccttgcctg
     1861 catggtaagt cccttgagtg ctggcggaaa aaaggaagag aagcgttctg tactcattat
     1921 tttagacgga aagggcttct ccgaatccat ccttgcgggt accccggagt atttagcgca
     1981 atggggctta acgtgcgata ttagcgcgcc agataaggag aatgtgataa gcgacacagg
     2041 aagaaatcgc aaggatgttc gatcccttac cgaggtcagc gatagtgatt attccgccct
     2101 tctgatagtt ccctctgctt ctcgaagttc actaatggaa gatcctgaat atgttgaaat
     2161 aatcggaaag ttctatcgtg caaaaaaa
//
LOCUS       DAOQXQ010000285         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57230_length_2185_cov_3.172770, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000285 DAOQXQ010000000
VERSION     DAOQXQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57230_length_2185_cov_3.172770"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /gene="mnmE"
                     /locus_tag="PKH81_05410"
     CDS             complement(<1..1132)
                     /gene="mnmE"
                     /locus_tag="PKH81_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNQ97509.1"
                     /translation="MKPSDYDPSRPIVAIASALVPQALGIVRLSGSRCLELLAPFFSR
                     PQALALAPGNTLVHGWLYNAPEGTEGRSRLDEVMIAVYRAPKSFTGEDSAEITCHGGP
                     AILMAVFRTAREAGFFPAQKGEFTLRAFMNGKTDLTRSEAIREIIESSTDMARESAAN
                     RLSGTLTREIIEIKNLVIHAIAAIGVEIEYPEDEETVKGAFNATPIIQAIERLKILES
                     AWVTERLLLEGVKIVLAGKTNAGKSRFFNALLKEERAIVSDTHGTTRDWLESRIDVDG
                     IPALLYDTAGMRETEDHIEAQGVDRSKDLARKADLILYLVDATCGLTDEDRQILSTLS
                     PSILVWSKVDLPQAKPLVNITQEGLPVQACIAASAKTGEGIHE"
     gene            complement(1151..>2185)
                     /locus_tag="PKH81_05415"
     CDS             complement(1151..>2185)
                     /locus_tag="PKH81_05415"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNQ97510.1"
                     /translation="IILASGTNAAATDKFAQLVLRLLGRRMEIDGRTHYLSVSIGIAM
                     YPDHGDSFDELIQKADAALHQAKKEGKKRYCFFDTRIQEELRHYVAIESALRNVVDNN
                     ELYTAYQPQVDILTGKIVGFEALARWNSFEHGEVSPGVFIPVAEATGQMDKIFSFILN
                     SVFAFIKRGSNLGYDSFEVSINVSAIQLEDEEFVPRIIRTIAEQGIPPQRICLEITES
                     LLISRMERTIQKLQSLRDAGIRLALDDFGQGFSSLSYLNQLPVHYLKIDKVFVDDILT
                     GENTRILTQNIIILAHQLGLTVVAEGVEEQEQINYLRDHTCDIIQGYCYSKPELEERA
                     LAKLELSFNP"
BASE COUNT          508 a          520 c          574 g          583 t
ORIGIN      
        1 gctcatgtat tccctctccc gttttggcgc ttgccgcaat gcatgcctgt accggtaagc
       61 cctcttgtgt gatgttcact agtggttttg cctggggaag atccaccttg ctccatacca
      121 ggatggaggg agaaagcgta gagagaatct ggcggtcttc gtcggttaat ccacaggttg
      181 cgtcaacaag gtacagaatc agatctgcct tacgagcaag gtcctttgat cgatcaacgc
      241 cctgtgcttc tatgtggtcc tcggtttcgc gcatgccggc ggtatcgtat aaaagggcag
      301 ggatgccgtc cacatcaatg cgggattcaa gccagtcgcg ggtggttcca tgagtgtcgg
      361 aaacaatagc ccgctcttct ttgagcaagg cgttaaagaa acgggactta cccgcatttg
      421 tcttgccggc caaaacgatt tttacgcctt ctaaaagaag tcgctcggta acccaggcag
      481 attccagaat ctttagtcgc tctatcgctt gtataatagg cgttgcgttg aatgcgcctt
      541 ttaccgtctc ttcatcttcc ggatattcaa tctctacgcc gatagcagca atcgcgtgga
      601 taaccagatt tttaatttca ataatctcgc gggttaaggt tccggaaaga cgatttgccg
      661 cgctttctcg ggccatgtcg gtggaggatt caataatttc ccgaatcgcc tccgatcggg
      721 ttagatctgt tttgccattc ataaatgcgc ggagggtaaa ctctcccttt tgtgcaggaa
      781 aaaaccctgc ttcgcgcgcc gtgcggaaga cggccataag aattgccgga ccgccgtggc
      841 aggtaatctc cgctgaatcc tctccggtaa aacttttagg cgcacgatag acggcaatca
      901 taacctcatc aagccgactt ctgccctctg ttccctctgg tgcattatag agccacccgt
      961 gaaccagggt gttcccgggt gccagagcga gggcttgtgg gcgggagaaa aagggtgcca
     1021 gtagttccag acatcgggag ccactgaggc gtacaatgcc aagggcctga ggaaccagtg
     1081 cacttgcgat agctactatg ggtctagagg gatcatagtc cgagggtttc atgggcgcag
     1141 tatagcaaat ttatggattg aaggacagtt caagtttggc aagggcgcgc tcttccagtt
     1201 cgggtttgct gtagcaatat ccctgtatga tgtcgcaggt atggtcgcgt agatagttga
     1261 tttgctcttg ctcttccaca ccttctgcaa caacggttaa gccaagttgg tgagcaagga
     1321 tgataatgtt ttgcgtcagg atgcgggtgt tttctccggt aaggatgtca tcaacaaaca
     1381 ccttgtctat tttaagatag tgtacgggaa gttgattaag atacgaaagc gaagagaatc
     1441 cttgaccaaa gtcgtccagg gcaaggcgaa tacccgcatc cctcaggctt tgtaattttt
     1501 ggattgttct ttccattcgg gatataagga gcgattcggt aatctcaagg caaatccgct
     1561 gaggggggat gccctgctcg gcgatggtgc ggatgatgcg gggtacaaac tcttcgtctt
     1621 cgagctggat tgccgatacg ttgatggata cctcaaagga atcgtagcca agattgcttc
     1681 cacgcttgat aaaggcaaag accgagttaa ggataaagga gaatatcttg tccatttgcc
     1741 cggtagcttc ggcaacgggg ataaacacgc cggggctcac ctctccatgc tcaaagctgt
     1801 tccatcgggc caaggcttca aagccgacaa tcttgccggt tagtatgtct acttgaggct
     1861 gataagcggt gtacagttca ttgttatcta ccacgtttct aagtgctgac tcaatggcta
     1921 catagtgtct cagttcttcc tgaatgcggg tgtcaaaaaa gcaatacctt ttttttcctt
     1981 ctttttttgc ctggtgcaag gcagcatccg ctttttgaat aagttcatca aaggaatccc
     2041 cgtgatcggg gtacatggca ataccaatgc tgacgctcag gtagtgtgtt ctgccgtcta
     2101 tctccatgcg gcggccaaga agacgtaata ccagctgagc gaatttatct gtagctgccg
     2161 cgtttgttcc gcttgccaag ataat
//
LOCUS       DAOQXQ010000286         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57259_length_2184_cov_2.718178, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000286 DAOQXQ010000000
VERSION     DAOQXQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57259_length_2184_cov_2.718178"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="ugpC"
                     /locus_tag="PKH81_05420"
     CDS             <1..1018
                     /gene="ugpC"
                     /locus_tag="PKH81_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNQ97511.1"
                     /translation="VVFVGPSGCGKSTTLRMVAGLEEITEGDLFIDGERMNDVPPKDR
                     NIAMVFQNYALYPHMTVYDNMAFGLKIRKTDKKEIERRVNEAARILDIEKLLDRKPKA
                     LSGGQRQRVAVGRAIVRNPKVFLFDEPLSNLDAKLRVQMRAEISDLHNRLKATMIYVT
                     HDQVEAMTMGDKIVVMKDGKVQQIGSPLYLYNHPINKFVAGFIGSPPMNFLTCKIVEK
                     GGQIVADEGSFEIVPTADQQKILKNYIGKEVYFGIRPEDLSYQKTPASKNNMQMKVVV
                     KEPLGAETHLILTTKTQQVIVRTSPDLVFQIGEAVNFQPLMEKAKFFDKDTELSICDD
                     VKAE"
     gene            complement(1075..2082)
                     /locus_tag="PKH81_05425"
     CDS             complement(1075..2082)
                     /locus_tag="PKH81_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ97512.1"
                     /translation="MSEHQFSPRQVIFAPTAVCNLNCGHCRVDRNRAHGEGTLSIEDA
                     KLFLQSCVDIGIERVGFSGGEPFLAIDFLEGIISKAVELDLYFDRLMTNGVWWNDEEI
                     LTSSIKRILDAGFDGILAVSLDDWHKQDSAQVCRFFSTAASLSGRYDWFEITSVTNPD
                     GTLSLDRLEQTAQGLKARLLYVNDKPAAIRDDRADKNRASGADDGSGIHIPISIITYS
                     PPASDTSSWKAERWFTDDWCEGPGQVLYVHPDGMVSVCCGFANESPLLIAGHISEGAS
                     TLFHRARTMPLVRDCYEKGLGTVRKELEQKGLPFPGKTGDMCQFCAWLYTQELYPSPK
                     K"
BASE COUNT          552 a          547 c          519 g          566 t
ORIGIN      
        1 cgttgttttc gtaggaccat ctggttgcgg aaagtccaca acccttcgta tggttgccgg
       61 tcttgaagaa atcaccgaag gtgacctctt tattgacgga gagcgcatga atgacgttcc
      121 tcccaaggac cgaaatattg cgatggtttt ccagaactac gctctgtatc ctcacatgac
      181 tgtttacgac aacatggcat ttggtttaaa gattcgcaag accgacaaaa aggaaattga
      241 gcgccgcgtt aacgaagctg ctcgtatcct tgatatcgag aaactcctcg atcgtaagcc
      301 caaggctctc tctggtggac agcgccagcg cgttgcggta ggtcgtgcca tcgttcgtaa
      361 ccccaaggta ttcctttttg acgaacccct gtcaaacctt gatgcaaagc ttcgcgttca
      421 gatgcgtgcc gagatctccg atcttcataa ccgacttaag gctaccatga tttatgtaac
      481 ccacgatcag gttgaagcga tgaccatggg tgacaagatt gttgtaatga aagacggaaa
      541 ggttcagcag attggatctc ccctgtacct gtataaccat ccgattaaca agttcgtagc
      601 aggctttatc ggatctccgc ccatgaactt ccttacctgc aagatcgttg aaaagggtgg
      661 acagattgtc gctgatgaag gatccttcga gatcgttccc accgccgatc agcagaagat
      721 tcttaaaaac tacatcggca aagaggtgta ttttggaatc cgtcccgaag atctgagcta
      781 tcagaagacc cctgcgtcca agaacaacat gcagatgaag gttgtggtta aggagcctct
      841 tggagccgaa acccacctta ttcttactac caagactcag caggttattg tccgcacctc
      901 tcccgacctg gtgttccaga ttggagaagc ggtcaacttc cagcccctca tggaaaaggc
      961 caagttcttc gataaggata ctgaactcag tatctgcgat gatgtcaaag ccgagtaaaa
     1021 agcggtaacg ctttagagta agccgtcctc agggacggct ttttttttga cctcttattt
     1081 ttttggggaa gggtagagtt cttgtgtgta gagccaggcg caaaactggc acatgtctcc
     1141 ggtttttccg gggaagggga gccccttttg ctctaattcc ttgcgaaccg ttccgagtcc
     1201 cttttcataa cagtccctga caagtggcat tgtgcgcgcg cgatgaaaca gggtggaagc
     1261 tccctcgctg atgtggccgg caataagaag cgggctttcg ttggcaaatc cgcaacagac
     1321 actcaccatc ccatccgggt gtacatacaa tacttggccc gggccttcgc accagtcatc
     1381 agtaaaccat cgttcggctt tccatgatga ggtgtcggaa gctggtggtg aataggtgat
     1441 gatgctaatc ggtatatgaa tccccgaccc gtcatcggca ccggaggcac gatttttatc
     1501 ggctctatcg tctcgaatag ctgcgggttt gtcgtttaca tataggagcc tggcttttaa
     1561 tccttgtgca gtctgttcaa gacggtctag tgaaagggtt ccgtcggggt ttgtaaccga
     1621 tgttatttca aaccaatcat atcttcccga taaggacgct gcagttgaaa aaaaacgaca
     1681 gacctgcgca ctgtcttgct tatgccaatc atcaagactt acggcaagaa tcccgtcaaa
     1741 tcccgcgtca agaattcttt taatcgaaga ggtcaggatt tcctcatcgt tccaccagac
     1801 accattagtc atcagtcgat caaaatacag gtcaagctct actgctttac tgataatacc
     1861 ttccaaaaaa tcaatcgcca gaaatggctc tccacctgaa aaaccaaccc gttcaattcc
     1921 aatgtcaaca cagctttgca aaaagagttt ggcatcttct atgctgaggg tgccctctcc
     1981 gtgagccctg ttacggtcta ccctgcagtg gccacagttc aggttgcaca cagcggttgg
     2041 cgcaaaaata acttgtcttg gggagaattg gtgttcactc atttactgct ccacgggtat
     2101 aaaaacgact gattgatccc ataattgtat gccgcttgcc cgcacggtaa tcgtatcatt
     2161 gcggctcgcg tttgatggag ccgg
//
LOCUS       DAOQXQ010000287         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57651_length_2174_cov_3.129778, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000287 DAOQXQ010000000
VERSION     DAOQXQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57651_length_2174_cov_3.129778"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1152)
                     /locus_tag="PKH81_05430"
     CDS             complement(82..1152)
                     /locus_tag="PKH81_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNQ97513.1"
                     /translation="MDYLESIACVIERSRVFSGRRVFVTIIANPAAGGFTRKKNAAYN
                     KAALAQAVRDVEGRAVVCTIASFAIEYTKASGHAREIADNVLVAALRADANDLYLIIT
                     AGGDGTSLEVQTSVARAILEAGHFSLSDRVCVLRLPFGTGNDGSDGRLLDQTLLLLTG
                     DSTLVKQSAVRAWGQGGEGFHYAFNIASVGLDAFVTHMTNRLKRLFPGNFYKIWLDIA
                     CLFYGKFFPVETMIVSAYDKSMSVVREEKEQFLICVMGASGRRTYGSNQKILPDDRNV
                     CAVRDLPLLKKLALKSRVKTGEHISFSEASLFEAHRIVIDYASPILVQFDGEAHLLTE
                     TAFPYVMELTDPCITVIRARQS"
     gene            complement(1158..>2174)
                     /locus_tag="PKH81_05435"
     CDS             complement(1158..>2174)
                     /locus_tag="PKH81_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ97514.1"
                     /translation="PGLVAVLSPEAAAALNIKSGTVSRVRIRIPQAVLERPADGTVSG
                     ATLSSQDSLTSSDPDMNPAAAVAMREVQDPVVPLSSIATNTDTDLIPASEVHETMPAE
                     VDQSLSEAASVAAEPVVETESLLMDGAAVVASTEEVSTPPSEETPEVAQTPEIPPEEI
                     PSEENPAFYEEPEVVLGEVEAVLVPTEPNPPEYAEVTEAESYLDGPAIPVVPVMPEVQ
                     DNSVDVEEVPAVEEAPVIAAVPVQPAPVAADTYSEDFPFVSSLQKGSWYVQIGSYAES
                     SSVRSILASWSKRYPLAIERGTVRGKDVMKVLVGPLGKDEYGAALERLRSSGFKDAFI
                     RKGK"
BASE COUNT          580 a          558 c          516 g          520 t
ORIGIN      
        1 aaaagttggc agaaacgaac cctgtccctg tggtagcgga aagaaataca aattctgtca
       61 cggtaaataa gccgttttcc gctacgattg gcgtgccctg ataacggtga tacatggatc
      121 ggtcaattcc ataacatagg gaaaggccgt ttccgttaac aggtgcgctt ctccgtcgaa
      181 ctgaaccagg atgggacttg cataatcgat aacaatgcgg tgagcctcga aaagcgaggc
      241 ttccgaaaat gatatatgct cgccggtttt aacacggctt ttaagcgcaa gtttttttaa
      301 taagggtaaa tcccgtactg cgcatacatt gcgatcatcg ggtagtattt tttgatttga
      361 tccgtaggtg cgccttccgg aagcccccat tacacaaata agaaactgct ctttttcctc
      421 gcgtacgaca gacatgctct tgtcatacgc gctaacgatc attgtttcca ctggaaagaa
      481 cttaccataa aaaagacagg caatatcgag ccatatcttg taaaaatttc cgggaaacag
      541 ccgtttaagc cgattggtca tgtgggttac aaaggcatca agcccaacac tggcaatgtt
      601 aaaggcataa tgaaagcctt ccccaccctg tccccatgcc ctgacagcac tttgcttaac
      661 aagagttgaa tcaccggtaa gcaacaacaa ggtttgatca agaagccttc catcagatcc
      721 gtcatttcca gtaccgaagg gcaacctgag aacgcatact cgatctgaaa gagaaaagtg
      781 accggcttcc agaatcgcac gggcaacaga tgtttggact tccagacttg tgccatctcc
      841 acctgcggtg atgattagat acagatcgtt tgcatctgcc cttagtgcgg caaccaggac
      901 attatcggca atctcgcgag catgacctga agcttttgtg tattcaattg caaaggaagc
      961 aatagtacag acaacagccc tgccttccac atcccttacc gcttgcgcca atgcagcctt
     1021 attgtaggca gcattctttt tacgggtaaa gcctccggcc gcgggattgg caataatggt
     1081 aacgaaaacg cgtcttccgg agaagacgcg cgatcgctca ataacacaag caatactttc
     1141 cagataatcc aattgcttta ctttcctttg cgaataaatg cgtccttaaa tccgcttgaa
     1201 cgaagccttt caagcgctgc gccatattca tctttaccaa gcggaccaac gagcaccttc
     1261 atgacatcct tgccccgaac agtaccccgt tcgattgcca agggatatct cttgctccag
     1321 gaagcgagga tggagcgaac cgacgaagat tcagcgtagc tgccaatttg cacataccaa
     1381 gagccttttt gaaggcttga gacaaaggga aagtcttcac tataggtgtc tgcagcgacg
     1441 ggagcaggtt gaacaggaac tgcggcaata acgggagcct cttctacagc aggaacttct
     1501 tcgacatcaa cactattgtc ctgtacctct ggcataaccg gcacaaccgg tatggcagga
     1561 ccgtcaagat aggattcagc ttcggttact tccgcatact cggggggatt tggttcagtt
     1621 ggaaccaaga ccgcttctac ctcgccaagg actacttccg gctcttcata aaaagccgga
     1681 ttttcctcag agggaatttc ctcaggcgga atttccggag tctgagcaac ttctggggtt
     1741 tcttctgagg gcggagtgct tacttcttcg gtacttgcta cgacagcagc cccatccatc
     1801 agtaaacttt cagtttctac gacaggctca gcggcaaccg aggccgcctc actcaaactc
     1861 tgatcaactt cggcaggcat ggtttcgtga acctcggaag ccggtattaa gtcagtgtcg
     1921 gtattagtgg ctatcgatga taaggggacc acaggatctt gaacctcccg catcgcaaca
     1981 gcggctgcag gattcatgtc gggatcacta ctggtaaggg agtcctgaga gctcagggtt
     2041 gctcccgaaa cggtaccatc tgcgggacgc tcaagcacag cctgaggaat tctgatgcgt
     2101 actctactca cggtgccaga tttgatatta agagcggcag ctgcttccgg agaaagaacc
     2161 gcgaccaatc cggg
//
LOCUS       DAOQXQ010000288         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57882_length_2169_cov_2.564806, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000288 DAOQXQ010000000
VERSION     DAOQXQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57882_length_2169_cov_2.564806"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKH81_05440"
     CDS             <1..718
                     /locus_tag="PKH81_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNQ97515.1"
                     /translation="CADLGIIIMLASGRAPEAMYQYAERLGLHTRSSYLICHNGSHAI
                     ESDTRKTILEHRLPTDIALEAFRLTLDAGLSCHIYEGDRIIVSRETAYSDRDKELSGL
                     IPIIPDDYEQVIRSGVYKLVIPGDPEFIVPVEAEFKVLFKDRATVFVSKPFFMEILPI
                     QAGKGEALRDISTGILGLPQAQVMAFGDSMNDESMIAWAGMSVAMSNSRREILHLARY
                     RTDLSNDEDGIADFLEKHVL"
     gene            754..1698
                     /locus_tag="PKH81_05445"
     CDS             754..1698
                     /locus_tag="PKH81_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ97516.1"
                     /translation="MLEIPINQDTSPEAVLDLLFRLKVRDVMTHPILTARSSDTMRQI
                     REIMRKNNIRGIPVADESMKLLGMVSMNDIINALDGGWMDDPAALHMTTKVIVLQESM
                     SLSFCVSYFNKWSFGRFPVLDEHSRLTGIVTSSDVISTLLVALNKELERMEQERVEEE
                     GSVSDQENPSQIQEERVIEFRTEPFNFGIAGQASTEIKRILKATGVDPSILRRIGIAS
                     YELEINQVVHSNGGIMRYRIQPDTLIIEAQDTGPGIPDVAKAMTEGFSTATERVRSLG
                     FGAGMGLPNTKRVSDDFAIESSTAGTLVTVSFRLGRQP"
     gene            1695..2027
                     /locus_tag="PKH81_05450"
     CDS             1695..2027
                     /locus_tag="PKH81_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97517.1"
                     /translation="MKTSELASFLAADCVQNEFHDKDIAGVYTSDLLSDAIANAPENG
                     ILVTIQAHKNTVAVGTLKDLAAIVICNNRSIPDDMKEAARLEGIALFVTADSQYLVSG
                     KLYQRLGA"
BASE COUNT          537 a          595 c          532 g          505 t
ORIGIN      
        1 ctgcgcagac ttagggatta tcattatgct cgcctcaggc cgtgccccgg aagccatgta
       61 tcaatatgcc gaacggctcg gccttcatac cagatcaagt tatctgatct gccacaatgg
      121 gtctcacgcc atagaatcag acaccagaaa aacgatcctg gagcatcggc ttcccaccga
      181 tattgccctg gaggcttttc gtctaaccct ggacgcaggg ctttcttgcc atatctatga
      241 aggagaccga atcatcgtct cccgggagac cgcctactcc gatcgagaca aggagctttc
      301 cggccttatc cccatcatac ccgacgacta cgaacaggtt atccgaagcg gtgtatacaa
      361 gcttgttatc cccggagatc ctgagtttat tgttccggta gaagctgaat tcaaggtgct
      421 gtttaaggat cgtgccacgg tctttgtgag caagcccttt tttatggaaa ttctccccat
      481 acaagccgga aagggcgagg cgctcaggga tatttccacc ggaatcctcg gccttcccca
      541 agcgcaggtt atggcttttg gagacagtat gaacgacgag tcgatgatcg cctgggccgg
      601 catgagtgtg gcaatgagta actcccgcag ggaaatattg caccttgcgc gctatcgaac
      661 cgatctttca aacgacgaag atggaatcgc cgattttctg gaaaagcatg ttctgtaaga
      721 aaaaaaacct cgcaatggta tatactcacc tgtatgcttg agatcccaat taaccaggac
      781 acctccccgg aggcagtgct cgatcttctc ttcaggctca aggttcgaga cgttatgacc
      841 catcccattc ttaccgcgcg ttcctcggac actatgcgcc aaattcgcga gattatgcgt
      901 aaaaacaata tccgcggcat tcccgtagcg gacgaatcca tgaaactgtt gggcatggtg
      961 tcgatgaacg acattattaa cgccctggac ggcgggtgga tggacgatcc tgcagccctc
     1021 cacatgacca ccaaggttat tgtccttcaa gagtcaatgt ccctttcctt ttgtgtctcg
     1081 tattttaaca aatggagttt tggtcgattc cccgttcttg acgaacattc ccgacttacc
     1141 ggcatcgtga cttcctcgga cgttatctcc actctcctgg ttgcactgaa caaagaactg
     1201 gagcgcatgg aacaggaacg cgtcgaagaa gaaggaagtg tatccgatca ggaaaaccct
     1261 tcccagattc aagaagagcg cgtcatcgaa tttcgcaccg aaccctttaa ttttggaata
     1321 gccggacaag cctccaccga aatcaagaga atcctcaagg ctaccggggt tgacccctcc
     1381 attctacgcc gaattggcat tgcgagctat gagcttgaga ttaaccaggt ggtccattcc
     1441 aatggcggga tcatgcgcta ccgcatacaa cccgacacct tgatcataga agctcaggac
     1501 accggacctg gcatacccga tgtggcaaag gccatgaccg aaggcttttc tacggcgact
     1561 gaacgagtgc gctcgcttgg cttcggggca gggatgggcc ttcccaacac aaagcgggtg
     1621 tcagacgatt ttgctattga atcaagcaca gccggaacgc tggttacggt tagttttaga
     1681 ttggggagac aaccatgaaa acaagcgaac tcgcgagctt tcttgcggcc gactgcgtac
     1741 aaaacgagtt tcacgacaaa gatatcgccg gagtgtatac ctccgatctt ttaagcgacg
     1801 ccatcgcgaa cgccccggaa aacggtattc tggttaccat acaggctcat aaaaacaccg
     1861 tcgcggttgg aacccttaaa gacctggcag ctatcgttat ttgcaacaac cgcagcatcc
     1921 cggacgatat gaaagaagcg gcccggcttg aaggcattgc cctattcgtt acagcggaca
     1981 gccaatacct ggtgtccggt aaactctatc agcggctggg cgcatgattg tccgttcgga
     2041 ctttcatatg cattcctgtc tttcgccctg tggggatctt tccatgtccc ccgctaccat
     2101 cgccttggta ctcagggaac ggggcgtacg gcttgccgcc cttaccgatc ataacagcgc
     2161 gcaaaactg
//
LOCUS       DAOQXQ010000289         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_57998_length_2166_cov_2.588820, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000289 DAOQXQ010000000
VERSION     DAOQXQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57998_length_2166_cov_2.588820"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKH81_05455"
     CDS             complement(<1..204)
                     /locus_tag="PKH81_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97518.1"
                     /translation="MKKTISLATKGLIIFLLVVLFGCKSTPPAEPETQVETAPEMNVQ
                     TVTDESVDPALVSLREQVEQRRNE"
     gene            311..535
                     /locus_tag="PKH81_05460"
     CDS             311..535
                     /locus_tag="PKH81_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HNQ97519.1"
                     /translation="MADDFSFEIVEKFGILSEGKGGWNTELNSVSWGGRPPKYDIRSW
                     SPDHQKMGKGITLSGEELAALKNLLTTIQV"
     gene            complement(543..1007)
                     /gene="rnhA"
                     /locus_tag="PKH81_05465"
     CDS             complement(543..1007)
                     /gene="rnhA"
                     /locus_tag="PKH81_05465"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNQ97520.1"
                     /translation="MSDLVIYTDGGCSGNPGPGGWGYVIVDDDRPADDREIIGSGGDS
                     QTTNNRMELTAVIEALTLVSTTGGWASRPVAVYTDSQYVQKGITQWITGWKRNGWRTA
                     SREPVKNQDLWIKLDSLAVMVKPRWVWVKGHAGIHYNEVCDRLTQEEISRHR"
     gene            complement(1004..2092)
                     /locus_tag="PKH81_05470"
     CDS             complement(1004..2092)
                     /locus_tag="PKH81_05470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNQ97521.1"
                     /translation="MNDNRIQDVRILEAQSLPSPEAMAQSFPVTPQAAQTVLKGRLDV
                     QRILRREDPRFLVVVGPCSIHSPDAALEYARRLVTLQKEYEDRMCIVMRVYFEKPRTT
                     VGWRGLIVDPGLDGTSDIARGLTMARQILISINDLGLPTGSEMLDPIVPQYTSDLVSW
                     ASIGARTTESQTHREMASGLSMPVGFKNATDGDIQIAVNAIVSSRQSHSFIGITREGK
                     ACIMRTLGNPDSHLILRGGRHGTNYDRESVTQAIALLKKSGVRPAVLVDCSHGNSQKM
                     PENQIGVLLETIRLRTDAKDPIAPLCGCMIESYLETGCQPISADPEDLKYGLSITDPC
                     LGWDMTASALAEAYRLLTKCSVKGKSRR"
BASE COUNT          553 a          557 c          501 g          555 t
ORIGIN      
        1 ttcatttcgg cgttgttcta cctgttccct taaggataca agagctggat cgaccgactc
       61 atcagtaacc gtctgaacat tcatctcggg tgcggtttca acctgagttt ctggctcggc
      121 aggcggggtc gacttacaac caaacagaac gactaacagg aaaataatca gtccctttgt
      181 tgcaagggat atcgtttttt tcatgggaac ctccacatgt attgtctgta gagtacactg
      241 tattgttttt taatacaaga attttttccc ccatggtata ctgggtggag atttaagagg
      301 agagaatgcc atggcggacg atttcagttt tgaaatagtg gagaaatttg ggattttatc
      361 cgaaggaaaa ggcggatgga acactgaact caactcagtc tcatggggag gaagacctcc
      421 caaatacgac atccgttcct ggtcacctga tcatcaaaaa atggggaaag gaatcacgct
      481 ctcgggcgag gagctagccg cgctcaagaa cttactcacc accatccagg tataacggac
      541 tgttagcggt gccgggatat ttcttcctgg gttaaacggt cgcacacctc gttgtagtga
      601 atgccagcgt gacctttaac ccacacccat cttggtttga ccattacagc taacgagtca
      661 agcttaatcc ataagtcctg attttttacc ggctcacgag aagcagttct ccaaccattt
      721 cgtttccatc cggttatcca ctgagtgata cctttctgaa catattgaga gtcggtatac
      781 actgccaccg gtcgcgaagc ccatccaccg gtcgtggaaa caagggttaa cgcttcaata
      841 acagcggtta gctccatacg gttattcgtt gtttgagaat ctccgcccga accgataatc
      901 tcgcgatcat cggcgggtcg atcatcatcg acgataacat acccccaccc gccagggccg
      961 ggatttcccg aacaaccgcc atcggtgtag attaccaaat cactcatcgt cgcgattttc
     1021 ccttaacaga acatttggtc aaaagccgat atgcctcggc cagcgcgctg gccgtcatat
     1081 cccagccaag gcagggatcg gtaatggaaa gtccatattt aagatcttcc ggatccgcgg
     1141 agattggttg gcaacccgtt tccagataac tttcgatcat gcaaccgcac aggggcgcaa
     1201 tgggatcttt ggcatcggtg cgcaatcgaa tagtttccag caatacaccg atttgatttt
     1261 ctggcatctt ctgtgaatta ccatgtgagc aatctaccaa gacagctggc ctgactcccg
     1321 atttttttaa gagcgcaata gcctgggtta cggattcgcg gtcatagtta gtaccatggc
     1381 gccctccgcg aagaataagg tgactatcag gattaccaag ggttcgcata atacacgcct
     1441 ttccctctct ggtaattcca ataaacgaat gagactgtcg ggaagagaca atggcgttta
     1501 ccgctatttg gatatctcca tcagttgcgt tcttgaatcc caccggcatt gaaaggcccg
     1561 atgccatttc gcgatgtgtt tgactttccg tagtcctggc cccaatcgat gcccaactca
     1621 ccagatccga cgtatattgg ggaacgatcg gatcgagcat ctcgcttccc gtgggcaatc
     1681 caagatcgtt tatactaatc aaaatttgac gggccattgt taatccgcga gcgatgtccg
     1741 acgttccatc caaaccggga tctacaatca acccacgcca tccaaccgtc gtcctgggct
     1801 tctcaaaata cacccgcatg acgatgcaca tgcgatcctc gtattccttt tgaagcgtta
     1861 caagtcgccg cgcatactca agagcagcat cgggactatg aatagagcaa ggaccaacca
     1921 ccaccagaaa ccggggatct tccctgcgta aaatccgctg aacatccaag cgacccttca
     1981 ataccgtttg agcggcttgc ggggttacgg gaaatgattg ggccattgcc tcgggagaag
     2041 gaagagactg tgcttccaaa atgcgtacgt cttgtattct gttgtcgttc attattagtt
     2101 tccgttaagg gaggtttttg cctgttcggt aattgtatca agcaactcca tcaccataat
     2161 ggaatc
//
LOCUS       DAOQXQ010000290         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58042_length_2165_cov_2.616588, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000290 DAOQXQ010000000
VERSION     DAOQXQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58042_length_2165_cov_2.616588"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKH81_05475"
     CDS             complement(<1..596)
                     /locus_tag="PKH81_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97522.1"
                     /translation="MNRYRTILCAALTLLVVSSVAMASDVDQMKLTVTPGETWSSRMW
                     IGPVPVKKTPQIAAWIETMEGSFVTTLTVSDATAKQKWKGKPEGGRPESLPVWTHATA
                     QSESTDAISSASPKNGLSVNNDGQSLEHGKPYRIKLEINTSFDYNEYWTKKSGNTGRY
                     SGVNGQPSLVYEVTFTAGTAGQYRLMPVGIGSVDGSSG"
     gene            complement(734..1978)
                     /locus_tag="PKH81_05480"
     CDS             complement(734..1978)
                     /locus_tag="PKH81_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ97523.1"
                     /translation="MKKPLVLIVFSLMCALVQAQSYPLQSAVSESAIQLSRSIPKNSR
                     VAVLNCTSSSPALSEYIVQELSFRLVSMNSFIIVDRANIALVKDELQFQLSGDVSDES
                     AQSIGKMLGAQIIITCSVDNALFLRGKAIEVETARVLAMSSTAITPDDLYTRLTATKK
                     ESGLITVSTTEELIAAIAPERTIKLTKGTYDLTTGWNTKNRYISWVDDYDGPTPIIKA
                     VSNLSLIGEEGVTIVIEPAYGWVISFDTCSDITISDIVIGHTVPGQCLGGVLRFKNCE
                     NIEIRSCDLYGSGTIGVELERTASFTMENSIVRECTYGLLNITKSSRVSFRNTVFRTT
                     GEYDLISVNSSDDVRWEDCDFLENWGSVLFNVDRASTAIVLSGGSIRENTIPRFQTNE
                     KTIRIQEVLFEDNGFIDPTQKH"
BASE COUNT          516 a          485 c          510 g          654 t
ORIGIN      
        1 tctcctgacg atccgtctac agatccaata ccaacaggca taagtctata ttggcccgct
       61 gttcctgcgg taaaggttac ttcgtagaca agcgaaggct gcccgttaac accggaatat
      121 cgacccgtat tacccgattt tttggtccag tattcgttat agtcaaaact ggtattgatc
      181 tccagtttaa tgcggtatgg ttttccatgt tccagacttt gtccatcatt gtttacagat
      241 agtccattct tggggcttgc cgagcttatc gcgtctgttg actcgctctg agcggttgca
      301 tgtgtccata caggaagtga ttctggcctt cctccttccg gcttcccctt ccatttttgt
      361 tttgctgtag cgtcgctaac ggttagggtt gtcacaaagg atccctccat tgtttcgatc
      421 cacgcggcta tttgcggtgt tttttttacg ggaacagggc caatccacat acgagaactc
      481 catgtctctc ccggtgtaac cgtaagcttc atttgatcta catccgaggc catagcaact
      541 gaacttacta caagcagggt tagtgctgca cacaaaatcg tcctgtacct attcatatat
      601 tactccttcc cgcgcataag cgggtatact aactcgatca ttcgatcggt gtattcaatt
      661 tgttcggctg ttgcttacgt gtttggctta aatcggcttt gtcaagagta catacgctcg
      721 tctccaggat tttttagtgt ttttgagttg gatctataaa cccattgtct tcaaaaagaa
      781 cttcctgtat gcggatggtt ttttcgttag tttgaaagcg aggtatcgtg ttctcccgga
      841 ttgatccacc agatagtact attgcggtac ttgctcgatc cacattgaat agaacagagc
      901 cccagttttc aagaaagtcg cagtcttccc agcgtacgtc gtcgcttgaa ttaactgaga
      961 ttaagtcgta ttcgccggta gtacggaaaa cggtatttct aaaggaaacc cgggaactct
     1021 tggtgatgtt caaaagtccg taggtacatt cacggactat cgagttctcc atggtgaaag
     1081 atgcggttcg ttcaagttct acgccaattg ttcccgatcc atacaggtcg catgagcgaa
     1141 tctctatatt ctcgcaattc ttgaatcgga ggacgccgcc aagacactgt ccgggaacgg
     1201 tgtgtccaat aacaatatcc gatatggtaa tatccgaaca ggtgtcaaaa gagataaccc
     1261 acccataagc tggctctata acgattgtta ccccttcctc tccgataagg gaaaggtttg
     1321 atacagcctt tattattgga gtagggccgt cgtagtcatc cacccaggag atgtaccgat
     1381 tctttgtatt ccatccggtg gtcaggtcgt aggtaccctt ggtcagtttg atggttcgct
     1441 cgggagcaat agctgcgatt agttcctctg tggttgatac ggtgatcaga ccgctttctt
     1501 tttttgtggc cgtcagtcgt gtatacaaat cgtcaggcgt aatagctgtg gaactcatag
     1561 caagaacccg ggctgtttca acttcgattg ccttaccacg gagaaacagg gcgttgtcta
     1621 cagagcaggt gataataatc tgggctccca gcatttttcc gatggattgg gccgactcgt
     1681 cgcttacgtc tccggaaagt tgaaattgga gttcgtcttt aacaagcgcg atgttagctc
     1741 gatctacaat gataaatgaa ttcattgaaa ccagtcgaaa cgatagttcc tgaacaatgt
     1801 attcgcttag cgcgggggag gatgaggtgc agttaagaac ggcgacccgg gagttttttg
     1861 gaatacttcg ggataattgg atggcacttt ccgagaccgc gctctggagc ggatagctct
     1921 gagcctggac aagggcgcac ataagggaaa acacgatcaa gacaagcggt tttttcatga
     1981 agtactccat cgcgctattg taggctactg ggacgatttt tacaatttca atttttcgat
     2041 tattctcttg tcgtattgtg cgcaatatta tattatacaa tatatcggag gtatatatgg
     2101 ctgatgtgtt atgggatctt gtagctaaac gggctaatgg tagtgattat gatttttccg
     2161 ccctt
//
LOCUS       DAOQXQ010000291         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58118_length_2163_cov_3.738615, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000291 DAOQXQ010000000
VERSION     DAOQXQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58118_length_2163_cov_3.738615"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1129)
                     /locus_tag="PKH81_05485"
     CDS             complement(92..1129)
                     /locus_tag="PKH81_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97524.1"
                     /translation="MKKHLFPVLVISLLFFFFLQVHAEQFEVVSGDLQLTLFPETGGF
                     ALRSISDRGKNRYEPLFEDRNYGSTSWFSVMCSGRVFKLARRSGNVPRVEEVPGGAKF
                     VFSLNDDFLVEQEFIFMASDSVSTKTSLRIETRIENISGKTLPFAAKALIDTMLGETE
                     GIHFSTDLRPRISTELKLMQGVDQDRYIQSAGKSQTLFIALSNETVTVPEEVYLANWD
                     RLNTLTWKPSFIEGRSYNTLYSVQDSAVLLVWPEKTIPAKGIYSVHMVLSTVPWRDTS
                     NPLKIVEDTPFSMDAVTSAPTLTAYARYERIQYLLGRIAEIEKNSNIASDEEISSLNR
                     ELDILLREEGN"
     gene            complement(1126..2079)
                     /locus_tag="PKH81_05490"
     CDS             complement(1126..2079)
                     /locus_tag="PKH81_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97525.1"
                     /translation="MKKRAFILLVLLSGMCVFARDPIMTGLDAYAASDWSTAILSFRK
                     AVSEKDASPEPWYWLVMAEISSGDYKSALIDIDRFIASFPKDPRIPDMMYQKGRVLFL
                     LGQHEKSIQALYSCISGWPNHQLISSAYYWMGENLYATGRFDEAKSIFRLILDSWPQS
                     VKREASWYRLAQLQESVREEELLKLLKMSHEESLKIIEDYQRREKTYEQAITAYQKRI
                     SDMIKDTRMGELENQLGEEKNRSSVLQDRIASLEIQNAELAAALAATGATIPASALSN
                     SMSEDLNSSDPEKRRLALEELRNKAQSLREMYDQILDGSSQ"
BASE COUNT          603 a          474 c          451 g          635 t
ORIGIN      
        1 cggctctaaa agggtcagta catcattata tcgcttgcgg tagaaagcgc gttttgcttt
       61 ttctaaaagg gtgtgtgcca ttgcctgtgt atcaattccc ctcttcacgt aaaagtatat
      121 cgagttccct gttcagggag gatatctctt catctgaagc aatgtttgaa tttttttcaa
      181 tttctgcgat acggccgagc aggtattgaa tccgctcata gcgagcatac gctgttaatg
      241 ttggggctga ggttactgca tccatcgaaa aaggagtatc ctctactatt tttaacggat
      301 ttgatgtgtc cctccacggt acagtagaga gaaccatatg aacagaataa atacccttag
      361 caggaattgt tttctcaggc cataccaata agacagcgga atcctgtaca gaatacaagg
      421 tgttatagga acgtccttcg ataaaagagg gcttccaagt aagtgtatta agacgatccc
      481 agtttgccag atatacctcc tctggcacag tgactgtttc atttgacaat gctataaaga
      541 gtgtttggct ttttcctgcg gattgtatgt atctatcttg atcaacacct tgcatcagtt
      601 ttagttcggt agaaattctt ggcctaagat ccgttgaaaa atggatacct tcggtttccc
      661 caagcatagt atctatcaat gcctttgcgg caaaggggag cgttttcccg gagatatttt
      721 caatgcgggt ttctattcgt aaagaagttt tggttgatac tgaatcgctt gccataaaga
      781 taaattcttg ttcaactaaa aaatcatcat tgagggagaa aacaaatttt gccccaccgg
      841 ggacttcttc aacacggggt acatttcctg agcgtctggc aagtttaaac actcgacctg
      901 aacacataac agaaaaccag ctggttgaac catagtttct atcctcaaac aatggctcat
      961 aacgattttt accccgatcg gaaatgctac gaagtgcgaa tcctcccgtt tcagggaaaa
     1021 gagttaattg gaggtctccc gatacaactt caaactgttc tgcatggact tgtaggaaaa
     1081 aaaagaaaag taaacttatt acgagtacag gaaacaggtg ctttttcact gactgctccc
     1141 atccagaatt tgatcgtaca tctctcgtaa agactgagct ttgtttcgta attcttcaag
     1201 cgcaagtcgt cttttctctg gatcggatga atttaaatcc tcagacattg aatttgataa
     1261 tgctgatgca ggaatcgtgg cgccagtggc cgcaagggca gctgcaagtt ctgcattctg
     1321 tatttccagc gaggcaattc tgtcctgaag taccgaagat cgattttttt cttcgcctag
     1381 ttggttttcc agctcaccca tgcgggtatc cttaatcatg tctgaaattc ttttctgata
     1441 ggcagttatt gcctgctcgt aggttttttc acgtctttga tagtcttcga taatcttaag
     1501 tgattcttca tggctcattt taagtaattt taacagctct tcttccctga cggactcttg
     1561 tagctgagcc aatctatacc aggaagcctc tcgtttaact gattgtggcc acgaatccaa
     1621 aataaggcga aatatacttt tggcctcgtc aaatcgtcca gtcgcgtaaa gattttcacc
     1681 catccagtaa tatgccgatg aaattaattg atgatttggc caaccagaaa tacaagaata
     1741 cagggcttgt attgattttt catgctgacc caaaagaaat aacactcgcc ccttttgata
     1801 catcatatcg ggaattcgcg gatcctttgg aaaagaggca ataaagcggt caatatcgat
     1861 gagtgcactt ttataatcac cggaggatat ctctgccatt acaagccaat accatggctc
     1921 aggcgaagcg tctttctccg agaccgcttt tctaaaagat aaaatcgccg ttgaccaatc
     1981 cgaagcggca tacgcatcca gtccagtcat tattgggtcg cgagcgaaaa cacacatacc
     2041 agaaagaagc acaagtaata tgaacgcgcg ttttttcatc aaagtctcca cgattgcggc
     2101 cgtttggcgc ggaaatccgc acggttcgct tacatttcac cggaaaagaa tgcagaccct
     2161 gac
//
LOCUS       DAOQXQ010000292         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58203_length_2161_cov_3.970560, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000292 DAOQXQ010000000
VERSION     DAOQXQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58203_length_2161_cov_3.970560"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKH81_05495"
     CDS             complement(<1..535)
                     /locus_tag="PKH81_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ97526.1"
                     /translation="MKVALVGYGKMGHMIERAALARSHEVVCTVDLFASDATCITSDS
                     REMIDAIRSSGAQGVIEFSHPASVMNNIQTLIPTGIPLVVGTTGWKDRLEEVSELVAL
                     NKTALLHASNFSIGVNLFYRMVREATRLMAAYEEYDTALFESHHNQKADSPSGTALDI
                     ARIVMDGNPRKNTLVTDA"
     gene            complement(532..1908)
                     /locus_tag="PKH81_05500"
     CDS             complement(532..1908)
                     /locus_tag="PKH81_05500"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ97527.1"
                     /translation="MIVMKFGGSSVANAERIRHVAQIIKAFADSRPIVVLSAMGDTTD
                     HLLEAADAALAGEIRIDAVRDLHIKTAQELNVPVTEIESLLAELTTLLTGISMLREVS
                     PRSRDYLVSFGERLSIRLMAGYLNSLPLRAEFKDAWDVGFASDSNFMNADLLPETWEN
                     IRKSLSPYANGKKGDGMPIPIVTGFIAKDPNGTITTLGRGGSDLTATILGAALGAQEV
                     QTWKDVDGILTTDPRVVKEAKTVPVATYDEVAELAYFGAQVLHPRSMQPCLQTGTPVR
                     VKNSYNIEAPGTIIVTSHPSTPSPVRAITSKKDVTVIDIVSTRMLGQHGFLAKVFEAF
                     AKHGISVDVVATSEVSISLTVDSKKVQFSNLRHELEKYASIDIRKGKAIVTIICDVKR
                     TSQILAASFEVLARGGINIQMLSQGASKVNISMICDSDEVDTVVRLLHGRYFGPNGED
                     LITEGVHT"
BASE COUNT          587 a          521 c          507 g          546 t
ORIGIN      
        1 aggcatcagt taccagggta ttctttcggg ggtttccgtc catcacaata cgggcaatat
       61 ccaaggctgt gccagaaggg ctatctgctt tttgattgtg atgagactca aacagggctg
      121 tatcgtattc ctcgtaagcg gccattaaac gagtagcctc gcgaaccatg cgataaaaaa
      181 gattaacacc gatcgaaaaa ttggaagcat gtaacaaggc tgttttattt aaggcgacca
      241 gctcgctcac ctcttccagt cgatctttcc agccagtcgt tcctactacc agagggatac
      301 ccgtaggaat aagagtttga atgttattca ttacagaggc cggatgagaa aactcaataa
      361 cgccttgcgc accagaggat cgaatcgcat caatcatttc ccggctatca gaggtaatgc
      421 acgttgcgtc ggatgcaaaa agatctaccg tacacactac ctcatgtgag cgtgcaagag
      481 ccgcacgttc gatcatgtgg cccatctttc catagcctac cagggctact ttcatgtgtg
      541 tactccctca gtaatcaaat cctcgccatt tggaccaaaa tatcgtccat gcaatagtct
      601 tacaacagta tccacctcat ctgaatcgca aatcatgctg atgtttacct ttgaagcgcc
      661 ctgactgagc atctggatat tgattcctcc acgagctaac acttcaaatg aggcagcaag
      721 aatctggctc gttcttttaa catcgcagat tatcgtcaca atagcctttc ccttgcgaat
      781 atcgatgctc gcgtattttt caagttcatg acgcaggttt gagaattgca cctttttgga
      841 atcgactgta agggatatgg atacctccga ggttgcaacc acatcaacac taatcccgtg
      901 ttttgcaaag gcttcaaaaa ccttggcaag gaaaccgtgt tggccgagca tcctggtaga
      961 gacgatgtcg ataacagtta catccttttt tgaggtaata gccctgactg gggaaggtgt
     1021 ggacggatga gaggtaacga taatcgttcc gggggcttct atgttgtacg aattttttac
     1081 ccgaacaggg gtgcctgtct gaaggcaggg ttgcatcgat cggggatgaa gtacctgggc
     1141 tccaaagtac gcaagttccg ctacctcgtc ataggtagcc acaggaacag tctttgcttc
     1201 ctttaccacg cgaggatcgg tggtaaggat accatcaacg tccttccagg tttgcacttc
     1261 ctgggcgcca agagcggcgc caagtatagt ggccgtcaag tcactgccgc cacgtccaag
     1321 ggtagtaata gtgccattag gatctttggc aataaagccg gtcacgatcg gaatgggcat
     1381 accatcaccc ttctttccgt ttgcataggg cgaaagggat ttgcgaatgt tttcccaggt
     1441 ttcgggtaac agatcggcgt tcataaaatt tgaatcggat gcaaaaccga catcccaggc
     1501 atccttgaac tctgcacgca agggcaagga gttaagatag cctgccatga ggcgaatgga
     1561 aagtcgctct ccaaaggaaa ccaggtaatc ccgggaacgt ggagaaacct cgcgaagcat
     1621 ggatattcca gttaaaaggg tcgttaactc tgcaagtaaa ctttctatct cggttacagg
     1681 aacattcaat tcctgcgcgg ttttaatatg caaatcacgt acagcgtcaa tccgtatctc
     1741 tcctgcaagg gcagcgtcag ccgcctcgag taaatgatcg gtggtatccc ccattgcgga
     1801 taacactacg atgggccggg aatcggcaaa ggcctttatg atttgagcta catggcggat
     1861 tcgttctgcg tttgcaaccg acgacccgcc aaatttcatt acgatcatat gtactctcca
     1921 cagtcataat tattcacact tgccaagaag ttatgcataa atataccaaa aagatgaata
     1981 gtgtacaata ctccctcgct gcccttgagt ggcaaagaag gtaaatgata gactcccgta
     2041 tgagtatttc tgaatacacc gttgtcctgt acaagggaca gcctgctctg gttaccggat
     2101 ttgataccgg caagataacc attcaaacta caggcggatc gataaaggtt cgcgaaaaag
     2161 a
//
LOCUS       DAOQXQ010000293         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58386_length_2157_cov_3.498097, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000293 DAOQXQ010000000
VERSION     DAOQXQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58386_length_2157_cov_3.498097"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKH81_05505"
     CDS             <1..635
                     /locus_tag="PKH81_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ97528.1"
                     /translation="DAARASNATQSKSMHPGAFLAALPSCASRAGLSPSDLIITTDVG
                     QHQMWSAQYYPVRTPRTFLTSGSLGTMGFGLPVAIGAALANPDKRVVCISGDGSIMMN
                     IQELATLNELGVNVTVIVLDNGSLGMVRQQQALLFNRHFSASVFESSVDLVAIASGFG
                     ITCARAEDEGWEKVAFAGSGPRFVRLAIDPEHMVSPFVPAGKANVEALLL"
     gene            664..1281
                     /gene="yihA"
                     /locus_tag="PKH81_05510"
     CDS             664..1281
                     /gene="yihA"
                     /locus_tag="PKH81_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNQ97529.1"
                     /translation="MVQWPFAGGKSVLKITDAEFIKGATRQSGYPSLGVSEFAFFGRS
                     NAGKSSLINMLLNRKSLVKTGSRPGMTRQINFFLVNKSFCLADLPGYGYAQRSQAENE
                     AFDRMLAEYVSTRETLKIIFLLMDLRREPKEAELNTVAYFEGLGKKVVLIGTKADKLG
                     TNDIAKTVRSWRTFFGDSDRQILVSSATKKNGRNDILALIAGELK"
     gene            complement(1282..1905)
                     /locus_tag="PKH81_05515"
     CDS             complement(1282..1905)
                     /locus_tag="PKH81_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNQ97530.1"
                     /translation="MNYTWQLHTIVNHGLGERIARETREAGARGGTILVGRGSAHQTI
                     LQLLALSDVEKDVLITLVTDEQYEAVLHAIRNSKTLNKKNRGILYAIPLCGGKQMSEE
                     SNYELITIITNRGYADDIMAAARKAGARGGTILHARGTGTEGDATFFGITVVPEKEQV
                     LILADGKTASNIKKAIASLPCLNKPGVGIMYATPVASFEQLGNGQGE"
     gene            complement(1902..>2157)
                     /locus_tag="PKH81_05520"
     CDS             complement(1902..>2157)
                     /locus_tag="PKH81_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HNQ97531.1"
                     /translation="SLALMAVCPKLFTAIAFDSGGVASGPLSNTFILALTLGASAGLG
                     GNPAMDAFGLIAMIAVTPIVSIQLLGWIYAHSAKRKEEQQ"
BASE COUNT          487 a          531 c          562 g          577 t
ORIGIN      
        1 acgatgcagc acgcgctagc aatgcgacgc aatcgaaatc catgcacccc ggagcctttc
       61 ttgccgcgct tccctcctgt gcctcccgtg caggcctttc tccctccgac ttgattatta
      121 ccaccgatgt aggccaacac cagatgtgga gtgcccaata ttatccggta cggacgcccc
      181 gaacctttct gacttctgga tcattgggaa ccatgggttt tggcctgccg gtggcaatag
      241 gcgctgccct tgccaatccg gataaacggg ttgtgtgtat ttcgggagac ggttctatca
      301 tgatgaatat tcaagaactt gctaccctga acgagcttgg agtgaacgtt acggttattg
      361 ttcttgataa cggttccctg ggtatggttc ggcaacaaca agctctgttg tttaatcgcc
      421 attttagtgc aagcgttttt gaatcatcag ttgatcttgt agcgattgcc tctggattcg
      481 gcattacctg cgcgcgggcg gaggatgagg gttgggaaaa agtggctttt gcaggatctg
      541 gcccgcgctt tgtccgtctt gcgattgatc ctgaacatat ggtgtccccc tttgttcctg
      601 caggaaaggc gaatgtagag gcactgctct tgtagactgg cttcttcccg cgacgggcga
      661 tgtatggtac agtggccttt tgcgggagga aagtcggtgc ttaagattac agacgcagag
      721 tttattaagg gcgccacacg ccagagcgga tatccatctc tcggggtaag tgaattcgct
      781 ttttttggcc ggtcaaacgc gggaaagtcg tcccttatca acatgctgct caaccgaaaa
      841 agcctggtta aaaccggttc ccgcccgggt atgacccgtc aaattaattt tttcctggta
      901 aacaagagtt tttgcctcgc agatttgcct ggatacggat atgcccaacg ctcccaggcg
      961 gagaatgaag cctttgaccg aatgcttgcc gaatatgtaa gtacacggga aacgctcaag
     1021 attatttttc tattgatgga tttgcgacgc gagcccaagg aagctgagtt gaatacagta
     1081 gcttattttg aagggcttgg aaaaaaggtc gttttaatcg gaaccaaggc cgataaactg
     1141 ggcaccaatg atattgccaa gactgttcgc tcatggcgta ctttttttgg agacagcgat
     1201 cgtcagattc tggtgagttc cgcaaccaag aaaaacggaa gaaacgatat tcttgccctt
     1261 attgcgggag aactaaagta actactcccc ctggccgttt ccaagctgtt caaaagacgc
     1321 aaccggtgtt gcatacataa ttccgactcc cggtttgttc aggcagggaa gacttgctat
     1381 ggccttttta atgttggagg ctgtttttcc atcggccagg ataaggacct gctccttttc
     1441 cggaacgacc gtaattccaa aaaacgttgc gtctccctct gttccggtgc cacgggcatg
     1501 gagtatggtc cctccacggg ccccagcctt tcgggcagca gccatgatgt cgtcggcata
     1561 accgcgattg gtgattatcg ttatcagttc atagtttgac tcttcgctca tttgtttccc
     1621 cccacataat ggtatggcgt atagtatgcc cctgttcttt ttattcagcg tttttgaatt
     1681 acgtatggca tgcaataccg cctcatattg ttcatccgtt accagggtga taaggacgtc
     1741 cttttctacg tcagagagcg caagaagctg caatattgtt tggtgagcgc ttcctctgcc
     1801 cacaaggatg gttcctccgc gggctcctgc ttcccgggtt tcacgggcga tgcgctctcc
     1861 aagaccatga tttacaatgg tgtgtaattg ccaggtatag ttcactgttg ttcctccttt
     1921 cgcttggccg agtgtgcata tatccaaccc agcaactgga tgcttacaat gggggtaacg
     1981 gcaatcattg ctataaggcc aaaggcgtcc atggcggggt tacccccaag accagcggac
     2041 gctcccaggg taagggcaag aataaaggtg tttgaaagcg ggcccgatgc gactccgcct
     2101 gagtcaaagg caatggcggt aaagagtttt gggcataccg ccataagggc aagggac
//
LOCUS       DAOQXQ010000294         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58570_length_2154_cov_2.604097, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000294 DAOQXQ010000000
VERSION     DAOQXQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58570_length_2154_cov_2.604097"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKH81_05525"
     CDS             complement(<1..475)
                     /locus_tag="PKH81_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ97532.1"
                     /translation="MSEYEGDLGAWRNQSLDDPIYLPVGIPVSFQVLIDTSPPQVAEA
                     LSRQVLPHPQESQVQDDESFDPLGEARYLVTPRLVHQYPSRVLLLSTGQCLGYCRYCF
                     RRGFIARSTGFISPQEQKDVCSWLSTHPEVREILVSGGDPLGAGINELERLLNGIH"
     gene            complement(468..1706)
                     /locus_tag="PKH81_05530"
     CDS             complement(468..1706)
                     /locus_tag="PKH81_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNQ97533.1"
                     /translation="MTPMEQLSSWIESQRSPMIDLQRALTAVPALAPESGGQGEKDKC
                     DLLTSALSALGYTQFERFDAPDPRVSCGYRPNLVVTIAGKDSSKTIWIMTHLDVVPPG
                     ELAKWNSDPWTVVEANGRVYGRGVEDNQQGMVASVFAGLSFIKTGIQPAYTVKLLFVA
                     DEEVGSAMGIQFLLQKHRLFRPDDIIIIPDGGDDCGSTIEIAEKNLLWLRVETKGIQT
                     HGSRPDQGANAHLAACDLALRLNALEEHFDRRDPLFEPDRSTFQPTKKEANVPNVNTI
                     PGDDVFYMDCRILPFYPLSLVRTEIARLVAEVEKKYGVVIQTREEQAVESRATSPDAP
                     VVRILAESIKRIYGVEAHPIGIGGGTVAAYLRNAGFDAVVWSKIDETAHQPNEYCVID
                     NLVGDARVMASVLLGGKDNE"
     gene            complement(1703..>2154)
                     /locus_tag="PKH81_05535"
     CDS             complement(1703..>2154)
                     /locus_tag="PKH81_05535"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ97534.1"
                     /translation="GLERNPQNLLPIVMEVAAGWRKELQIYGNDYDTRDGTCIRDYVH
                     VSDLAQAHVNALAYIVREQKSLTVNLGSESGVSVTEMVEAARRVTGKPIPATYTARRP
                     GDPSALYATSERARSLIGWNPTRSDVDTLISSTWAVYQYAIKEGKTL"
BASE COUNT          530 a          568 c          559 g          497 t
ORIGIN      
        1 gatgaatgcc gttaagcaag cgctcaagtt cgttgatacc tgcgccaagg ggatcccctc
       61 ccgataccag aatctcgcga acctccgggt gagtcgagag ccagctacag acatcctttt
      121 gctcttgagg gctaataaat ccggttgaac gggcaataaa acctcgtctg aagcaatacc
      181 tgcaatagcc cagacactgg ccggttgaaa gcaacaatac ccgcgatgga tattgatgca
      241 ccaaacgcgg tgttaccaga taccgggcct cgcctaatgg atcgaaagat tcatcgtctt
      301 gaacctggga ttcctgtgga tgtggaagca cctggcggga gagagcttcg gcgacttgag
      361 gagggcttgt atcgataagc acttgaaagg atacaggaat acctaccggc aggtaaatag
      421 ggtcatcgag cgactgattg cgccaggcgc caaggtcgcc ttcatattca ctcattgtcc
      481 ttgccaccca acaaaaccga ggccataacc cgagcgtctc ccacaagatt gtcgataaca
      541 cagtattcat ttggctgatg ggctgtttcg tcaatcttgc tccacactac cgcgtcaaac
      601 ccggcattgc gcaggtacgc agcaacagtc cccccgccaa ttccaatggg atgcgcctct
      661 actccatata tgcgcttaat cgattcggca agaatacgca ctacgggagc atcgggtgag
      721 gtggcacgag attccaccgc ttgctcttcg cgagtctgga ttaccacccc gtattttttt
      781 tctacttctg ccacaagacg ggctatttcc gtgcggacaa gggacagagg atagaagggc
      841 aggattcgac aatccatata aaagacgtcg tcgccgggaa tggtattaac gttagggacg
      901 tttgcttctt tttttgttgg ctgaaaggtt gaacgatccg gctcaaacaa tggatcgcgc
      961 ctgtcaaagt gttcttccag ggcattgagc ctaagggcaa ggtcacaggc ggcaagatgg
     1021 gcgtttgcac cctgatccgg ccgggagccg tgggtctgaa ttcccttggt ctctacccga
     1081 agccacagca agttcttctc cgctatttct atcgttgatc cgcaatcatc gcccccatcc
     1141 ggaatgatga taatatcatc agggcgaaac aaacgatgct tttgaagaag gaattggatg
     1201 cccatggcag agccgacttc ttcatcggcg acgaataaaa gttttacggt gtacgcaggc
     1261 tgtatgcccg tcttgataaa ggatagtccg gcaaacacag aagcgaccat gccctgctga
     1321 ttatcttcta ccccgcgacc gtatacccgg ccattagctt ccactacggt ccagggatcg
     1381 ctattccact tggcaagctc gccaggaggg acaacatcaa gatgggtcat tatccagatg
     1441 gttttggaac tatcttttcc cgcgatggtt acgactaaat tgggtcgata gccacaagaa
     1501 acccgaggat cgggagcgtc aaaacgttca aactgggtat atccaagtgc agaaagggcg
     1561 gaggttaaca gatcgcactt atccttttcg ccctgccctc cggattcagg tgcaagggcc
     1621 ggcaccgcgg taagcgcacg ctggagatct atcatcggtg agcgctggct ttctatccag
     1681 gaggaaagct gttccattgg ggtcataggg tctttccttc cttaatcgca tactgataga
     1741 cagcccaggt cgacgatata agggtatcta catcggagcg agtgggattc cacccgatga
     1801 gagagcgagc acgctcggag gttgcataga gtgcagaagg atcgccaggg cgacgagcgg
     1861 tgtaggtagc ggggattggc tttccggtta cccttctcgc ggcctctacc atctcggtaa
     1921 cggacacgcc gctctcgcta cccagattga cggtaaggct tttttgttcc cgaacgatat
     1981 atgcaagggc attcacatgc gcctgtgcta aatcacttac atgcacataa tcgcgaatac
     2041 aggtaccgtc gcgggtgtca taatcatttc cataaatctg aagttcctta cgccaaccgg
     2101 cagccacttc catgacaatg ggtaagagat tttgaggatt cctctccaag cccc
//
LOCUS       DAOQXQ010000295         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58619_length_2153_cov_2.220686, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000295 DAOQXQ010000000
VERSION     DAOQXQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58619_length_2153_cov_2.220686"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PKH81_05540"
     CDS             complement(<1..1340)
                     /locus_tag="PKH81_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ97535.1"
                     /translation="MKSHSATLFLVPVLLVAGSASLFANEGSVAEHMSLLVLQIGVIL
                     FAVRFFGFLVKKIGIPSVLGELLAGVIIGPFALGAIPLPGLPLGLFPVQASTLAVSTE
                     LYGFATVASILLLFASGLETDLALFLRYSVAGGIVGLGGVIFSFSLGGIAGMLLLDAP
                     FMDSRCLFLGILSTATSVGITARILSDQKKMDSPEGVTILAAAVFDDVLGIIMLAIVL
                     GFVTAVSGSSSAHGVDPALIGMIAAKAFGIWLGFTALGLVFHKQLARFLKLFRHSYDF
                     SICALGIALLLAGLFEKQGLAMIIGAYVTGLSLSKTDIATVIQERIHGLYEFFVPLFF
                     AVMGMLVNVRELFSVDVILFGLLYTLLSVASKVFGCGLPALGLGFNVKGALRIGAGMV
                     PRGEVALIIAGIGITAGILNDQLFGVVIMMTLLTTLFAPPLLNATLNLKGRGTR"
     gene            complement(1413..>2153)
                     /locus_tag="PKH81_05545"
     CDS             complement(1413..>2153)
                     /locus_tag="PKH81_05545"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNQ97536.1"
                     /translation="YGFPSVACIVQDKLGLTQAGAFDVVAACSGFVYALDAAGGMLTG
                     NRKRALVIGADVLSRITDWTDRSTCVLFGDGAGAVLIERTDAPASGEGKRGIIASILG
                     SQGSGAQELICLRGGSRHPFKAGETIDKPIHLYMNGRAVYTFAVKAFTDTIEALLEKE
                     GLTLDQVKKIIPHQANMRIIQAAAKRLSIPEDKFFMNIEEYANTSNATIPLALDDYNR
                     TGELKRGDVIMTVGFGGGLTFAGNLIVW"
BASE COUNT          629 a          545 c          555 g          424 t
ORIGIN      
        1 ttgcgcgttc cccgaccctt tagatttagc gtcgcgttta gaaggggagg ggcgaacagt
       61 gtcgtcagga gggtcatcat gataaccaca ccaaaaagct gatcgttcaa aatgccggcg
      121 gtaataccaa taccggcgat aatcaaggct acttctccac ggggtaccat accggctccg
      181 attctgagtg cgcctttaac attaaagcca aggccaaggg caggtagtcc gcacccaaag
      241 acctttgatg caactgaaag aagggtgtac aagaggccaa agaggataac gtcaacagaa
      301 aacaactctc gtacgtttac gagcataccc ataaccgcaa aaaagagggg aacaaagaat
      361 tcataaaggc cgtggatacg ctcctgaata accgtggcga tatctgtttt ggatagcgat
      421 aaacccgtta cgtacgcgcc aataatcatg gcaagtccct gtttttcaaa cagaccggca
      481 agtaaaagag cgattccaag ggcgcagatg gaaaagtcat acgaatggcg gaagagctta
      541 agaaatcggg caagctgttt gtgaaacacc aggccaaggg cagtaaagcc aagccaaatg
      601 ccaaaggcct tagccgcaat catgccgatt agggccggat ccaccccatg tgccgaggaa
      661 gagccggaga cggcggtaac aaagcccaga acgattgcca acatgattat gccaagcaca
      721 tcgtcaaaaa ccgcggccgc gagtattgtt accccttccg ggctatccat ctttttttga
      781 tccgagagta tccgtgcggt gataccgacg gaggtcgcgg ttgacaggat accaagaaag
      841 agacagcggc tgtccataaa gggtgcgtca aggagcagca tccccgcaat accacccagg
      901 gaaaaagaaa agataacccc accaaggcca actataccgc cggcaacgga gtagcggaga
      961 aacagggcca ggtctgtttc aaggccagag gcaaaaagga gcaagatgga tgcaaccgtg
     1021 gcaaaaccat agagttcggt actgaccgcc agggtagatg cctggaccgg gaatagtccc
     1081 aggggtaatc ccggtaaggg aatagccccc agagcaaagg ggccgataat aacaccggca
     1141 aggagctcgc ccaagaccga gggaatgcca attttcttga ccagaaatcc aaaaaaccga
     1201 accgcaaaga ggatcactcc aatttggagg accaacagtg acatgtgttc ggcaactgag
     1261 ccttcattgg caaaaagtga agccgagccc gcgacaagca ggaccggcac caaaaatagg
     1321 gtagcagaat gtgatttcat atatgcctcg tagagttaaa aacgcgcgaa gcatgacata
     1381 ttatagaaaa cagtgcaata gggtgtagat ttttaccaaa cgataagatt tcccgcaaag
     1441 gtaagtccgc caccaaagcc tacggtcatg attacatctc cccgtttcag ttcaccggta
     1501 cgattatagt catcaagggc aagaggaatc gttgcattgg aggtgtttgc gtattcttct
     1561 atgttcataa agaacttatc ttcgggaata ctgagccgct ttgccgcagc ctggataata
     1621 cgcatatttg cctgatgggg gatgatcttt ttaacctgat caagggtaag gccttctttt
     1681 tccaaaaggg cttcgatggt gtcggtaaaa gccttgacgg caaaggtgta caccgcgcga
     1741 ccgttcatat acaggtgaat tggcttgtcg atcgtttcgc ctgctttgaa cggatgcctc
     1801 gatcctcccc gcaggcatat caactcttgg gctccagatc cctgtgaccc taaaattgaa
     1861 gcaataatac cccgtttacc ctctcccgat gcgggtgcgt cggtgcgttc aataagtacc
     1921 gcgccggctc cgtctccaaa gagtacgcag gtgcttcgat cggtccagtc ggtaatgcgg
     1981 gaaagcacat ccgctccaat gacaagggct ctctttctgt tcccggtgag catacccccc
     2041 gcggcgtcca gggcatagac aaaacccgaa caagcggcga ctacgtcaaa ggctcccgcc
     2101 tgggtaaggc ccagtttatc ctgaacaata caggcaacag acggaaaacc gta
//
LOCUS       DAOQXQ010000296         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58859_length_2147_cov_2.952677, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000296 DAOQXQ010000000
VERSION     DAOQXQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58859_length_2147_cov_2.952677"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      16..190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            234..>2147
                     /locus_tag="PKH81_05550"
     CDS             234..>2147
                     /locus_tag="PKH81_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNQ97537.1"
                     /translation="MPSRTLCALIFSVLLCLPVVTTSADEIPENRYVVTATREEADTL
                     ETPAVIRVITQDDIAASGKTSLISLLEDTAGVSFKSFSTEAQSQIAMRGFGDNSFGRV
                     AILVDGKKINNPDMAGINWLSIPLAAIERIEVLDGPAAVTYGSGAIGGVINIITRGGT
                     PGLTAEASLSWGSFDTKEALLTGGWGGERAGFLVSADLLHSGGYRDETAIESTNIHLG
                     SYVAFGDYLMVKPALSWSNVSFELPGGLTKEQDKQDPTQSLFSDGKGDESGWTVSLST
                     NYDRDGKLTVDFPLEYLSRQRSADIPSSWGYSYYDYDQSQFSARPRFGIDIAPAGLPS
                     RITVGLDGEYCQYSAEQWGEAERTLRLSEYEFIQSSISPWLTARLSPTEALILQAGFR
                     LHWNQLETNSATGTGNGDTDSTDTAFEGSISWRLSKAVSLHVKGNTLFRIPFIDEVLT
                     YGTFLDHLESERGWNTEIGIKYMSDERLSLQAVFYHLLMEDEIAYNMQTMRNENLDRT
                     RRWGLTADGQWQPLPWFLLSGNLAWVHAIFDAGIHEGENIPLVPSLTAGGSLSFFLPQ
                     GVTLGADITWTGETDDPGSLDMPDVYGTIDSYALLGAQVHWKVPQADGKLSVTARVNN
                     ILDLTVSPLVYWGT"
BASE COUNT          498 a          575 c          538 g          536 t
ORIGIN      
        1 tgcgctctct atgataccaa cacacatact aaaggatatg gggaaaacgg aatgcggtgt
       61 aaatccgcag cggtcccgcc actgtaagca gaaaagcgcg ataacgccat cgggaaggct
      121 ccgtcccgag aaggccgcgc cgccatacgc gctgcaagcc aggagacgat cccctatttc
      181 cacacactca tctttgtccc gatgtggaca ttggcatttc ccgaggaggt tttatgccgt
      241 cgcgaactct ttgtgctctc attttttctg ttctattgtg tcttccagtc gttacaacgt
      301 ctgccgacga aatccccgaa aatcgttatg tggtaaccgc tacccgcgag gaagccgata
      361 ccctggagac tccggccgtt atcagggtta ttacccagga tgatattgca gcctctggta
      421 agacaagcct gatctctctt ttagaagaca ccgcgggcgt ttcctttaag tctttttcta
      481 ccgaagccca gtcccaaatc gctatgcgcg gctttggcga caattctttt ggccgagtag
      541 ctatccttgt ggacggaaaa aaaatcaaca accccgatat ggccgggatt aattggcttt
      601 ccataccgct tgcggccatt gaacgaatcg aagttcttga tggtcctgct gcggttacct
      661 atggatcagg tgctattggg ggggtaatca acattattac ccgcggaggg acccctggtc
      721 ttaccgcaga ggcctccctt tcgtggggaa gttttgacac caaagaagcc ttgctcacgg
      781 gtggatgggg aggcgagcgt gcgggatttt tggtctcggc cgacctttta cacagcggag
      841 gctaccgcga tgaaacagcg atagagtcaa ccaacatcca tctgggttcc tatgtggcct
      901 ttggggacta tcttatggtt aaacccgcac tttcctggtc gaacgtttct tttgagctcc
      961 ccggcgggct aaccaaagag caagacaaac aagatccgac acagtctctt ttcagcgacg
     1021 gcaagggtga tgaatcaggg tggacggtat cgctttctac caactatgac cgtgatggaa
     1081 aactgactgt agatttcccg ctggaatatc tttcccggca acggagcgcg gatatccctt
     1141 cgtcatgggg gtatagttat tatgattatg atcaaagcca gttttcagcc cgccctcgct
     1201 ttggtataga tattgccccg gcaggtcttc cctcgcgcat aacggttggc ctggatggag
     1261 agtattgcca atacagcgca gaacaatggg gcgaggccga gcgcaccctt aggctttccg
     1321 agtacgagtt tatccaatca tcaatttccc cctggcttac agctcgacta tcgcctacag
     1381 aagcacttat ccttcaggct ggcttccgcc tccactggaa tcagctcgaa accaatagtg
     1441 ccaccgggac tggaaacgga gacacggatt caacagacac tgcttttgaa ggatccattt
     1501 cttggcggct ttccaaagct gtatccctac atgtaaaagg gaacaccctg tttcgcatcc
     1561 cctttattga tgaagtgctt acctatggta ccttcctgga tcatttggaa agcgaacggg
     1621 gatggaacac agaaatcggt attaagtata tgagcgacga aaggctttcg cttcaggcag
     1681 tgttctatca tcttctgatg gaggatgaaa ttgcatacaa catgcaaacg atgcgaaacg
     1741 aaaatcttga ccgtacccgc cgctggggtc ttaccgccga tggacagtgg caaccgctcc
     1801 cctggttttt gctgagcgga aatctggctt gggttcacgc catctttgat gcggggatac
     1861 acgaaggaga gaacatcccc cttgtcccct ctcttaccgc aggaggctct ctttctttct
     1921 tcctgcccca aggcgttacc cttggtgcgg acatcacttg gacaggagaa acagacgatc
     1981 ccggttccct ggatatgcct gatgtgtacg gaacaatcga ttcctacgcc ttgttaggag
     2041 cccaggtcca ctggaaggtt ccacaggctg acggaaagct ttccgttacc gcccgagtaa
     2101 acaatatcct tgatcttacc gtctctcccc ttgtctattg gggcacc
//
LOCUS       DAOQXQ010000297         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_58903_length_2146_cov_2.976088, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000297 DAOQXQ010000000
VERSION     DAOQXQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58903_length_2146_cov_2.976088"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="fliN"
                     /locus_tag="PKH81_05555"
     CDS             <1..307
                     /gene="fliN"
                     /locus_tag="PKH81_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNQ97538.1"
                     /translation="QSVQFPNLQMNSMSMEQGNIGLIMDVFMEMTVELGRTRKVIKEI
                     LGMGEGTIIELDKLAGEPVDILVNHKPIAKGEVVVIDENFGVRVTEILSPIERVTDM"
     gene            339..1001
                     /gene="fliO"
                     /locus_tag="PKH81_05560"
     CDS             339..1001
                     /gene="fliO"
                     /locus_tag="PKH81_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNQ97539.1"
                     /translation="MLINTFGGNTPLRALCICAALVFSFYSLPSIHAQDAERAEEQAI
                     ILDNQDVSSPLAGSEQGESSIWVLLRVVLVLVVVCAAIYAVVYLLKKSTKINAANDPY
                     LKSVASLPLAPNKGLQVITLAGRAFLIGYTDHSINLIGEIDDKELIDAMNLDAERVMP
                     PVAANFASLLARFLPTPTKTGSPESAEEIPDTADFIKRQRERLRGMSGDTPQSDSSRS
                     QV"
     gene            998..1828
                     /gene="fliP"
                     /locus_tag="PKH81_05565"
     CDS             998..1828
                     /gene="fliP"
                     /locus_tag="PKH81_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758725.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNQ97540.1"
                     /translation="MSISLRRVCCVSLIAFFICIAPALLSAQTNPLPQGSARGTTAMN
                     AERQATRIPFVNLEIREPQSNREVALSVQLLLLLTLLSLAPSILILTTSFLRITIVLD
                     FVKRALSLQQVPPTQVLNGVALFLTIFIMWPTFQTIYDRSFRPLADGQIGVEDAYREA
                     EGPLRMFMYRQMSQDTSSIALFMSMSRLEKPQTLADVPTRVLIPAYILHELTVAFKIG
                     ILLYIPFIIIDMVVASILMSMGMIMLPPVQISMPFKLILFILVDGWGLLSQQLFNSFL
                     "
     gene            1847..2116
                     /gene="fliQ"
                     /locus_tag="PKH81_05570"
     CDS             1847..2116
                     /gene="fliQ"
                     /locus_tag="PKH81_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNQ97541.1"
                     /translation="MSLGMVVSMMRSGIFEILILSAPILFAALTVGLIVAIFQATTSI
                     QEQTLTFVPKILTILGMLALLGGWMFASLREYTINLFEMIPQMVR"
BASE COUNT          523 a          478 c          489 g          656 t
ORIGIN      
        1 acaatcagtg caattcccca atcttcaaat gaattcaatg tctatggaac agggcaatat
       61 tggccttatc atggacgttt ttatggaaat gaccgtagaa ttgggccgaa cccgtaaggt
      121 tattaaagag attcttggaa tgggagaagg taccatcata gagctcgaca agctcgctgg
      181 tgagccggta gatattttgg ttaatcataa accaatcgcc aagggtgaag tcgttgttat
      241 tgacgaaaac ttcggcgtcc gtgtaaccga gattctctct ccaattgaac gggtaacgga
      301 tatgtagccg aggcactata cggggtgggg ggattttcat gttaatcaac acgtttggtg
      361 gaaatacgcc tttgcgtgcg ctgtgtatat gcgccgcatt ggtgttttct ttttattcgc
      421 tcccgtctat tcatgcacaa gatgcggagc gagcagaaga acaggcaatt attctagata
      481 atcaggatgt aagctcacca ttggccggct cggaacaggg tgaaagtagt atttgggtgt
      541 tactccgcgt cgttcttgtc cttgtggttg tgtgtgcggc aatttacgcg gttgtgtatc
      601 ttcttaaaaa aagtacaaaa atcaacgcag caaacgatcc ctatcttaaa tctgtggcat
      661 ctcttccact tgcgcctaat aagggtttac aggtaatcac tcttgccgga cgcgcttttt
      721 taataggata caccgatcac tcgattaact tgattgggga aatagacgac aaggaactca
      781 ttgatgcgat gaatctggat gcagagcggg taatgcctcc tgtggcggct aattttgcct
      841 ctcttcttgc gcgattttta ccaactccta caaaaacagg atctccagag tctgccgaag
      901 aaattcccga tactgccgat tttatcaagc gacagcggga gcgccttcgc gggatgtccg
      961 gtgacacacc acagtcagat tcttcaaggt cgcaagtatg agcatatcat tacgccgtgt
     1021 gtgttgtgtc tcgttaatag cattctttat ctgtattgca cccgctttgc tttccgcgca
     1081 gacaaatccg cttccgcagg gaagtgctcg gggaacaacc gccatgaatg cggagcgtca
     1141 ggcgacccgc attccctttg ttaatcttga aattagggaa cctcaatcca atcgtgaagt
     1201 agctctttcg gttcaattac tgctgctttt aacccttctt tctctagcac cgagtatttt
     1261 aattcttacc accagttttc ttcgtattac tatcgttctt gactttgtta aacgagcctt
     1321 gtctcttcaa caggttccac ccacgcaggt ccttaacgga gtcgcgctct ttttaacgat
     1381 ttttattatg tggcctacct ttcaaacgat ttatgatcgt tcgtttagac cgctcgccga
     1441 tggacaaata ggcgttgagg atgcctatcg tgaagcagaa gggcccctgc gtatgtttat
     1501 gtaccggcag atgtctcagg atacctcgtc aatagccctg tttatgtcaa tgtcccgtct
     1561 tgaaaaaccg caaacacttg cggatgtgcc aactcgggta ttaatacccg catacattct
     1621 ccatgagctc actgttgctt ttaaaatagg tattctgtta tatattcctt ttattattat
     1681 cgatatggtc gtggcgagta tccttatgtc aatgggtatg atcatgcttc ctccagtaca
     1741 gatttcaatg ccatttaagc tcattctgtt tattctggtc gatggatggg gcctgttatc
     1801 gcagcaattg tttaattcat ttttataagc ggggaggagt aaacgcatgt cattaggaat
     1861 ggttgtaagt atgatgcgtt cgggaatatt cgagatttta atcctatccg ctcctattct
     1921 ttttgccgct cttacggttg gtttaattgt agcgatattt caggcaacta catcaatcca
     1981 ggaacaaacg cttacctttg ttccaaaaat tctgacgatt cttgggatgc tggccttgct
     2041 tggaggttgg atgttcgcct cgcttcgcga atacaccatc aatctcttcg agatgatacc
     2101 ccagatggtt cgataatctc cgcagaaatg agctgccgcg atattg
//
LOCUS       DAOQXQ010000298         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59008_length_2144_cov_2.174246, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000298 DAOQXQ010000000
VERSION     DAOQXQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59008_length_2144_cov_2.174246"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKH81_05575"
     CDS             <1..338
                     /locus_tag="PKH81_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNQ97542.1"
                     /translation="AIMLVIVLRFIPTFGERMRQVLQAEAARGYNVESGNPFRKIGVI
                     LRLSLPLLISCARDVDTLALSIETRGFDPRNTTRPRRIPPTRGDALVLILCLGLQTAI
                     VLADRIVCT"
     gene            351..2105
                     /gene="iorA"
                     /locus_tag="PKH81_05580"
     CDS             351..2105
                     /gene="iorA"
                     /locus_tag="PKH81_05580"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNQ97543.1"
                     /translation="MKTLLTGNEAVALGAWEAGVRAAYGYPGTPSTEILENLSRHSKD
                     VYLEWSPNEKVAFEAAAGSSYAGVRTIVTMKHVGLNVAADPLMTLSYVGTEGGFLICV
                     ADDPGQHSSQNEQDSRHFARFAKIPVLEPANSDEALRFMKYGFELSERFKTPVMIRLT
                     TRVSHSRGLVETGNRIESKEAHFERNPSRFCPLPIWGREMRKKVEIRTGDLAQEAAQS
                     PLNRIEMADTSLGIIAEGLAWNYVKEVFPTASVLKLGWAWPYPDSLIRDFASQVQRLV
                     VIEENEDILEEHIKAMGIACTGRDLLSGVGELSVPKLLALKAKLEGNEHPVLPAPIEA
                     AKDLPARPPVLCPGCPHRGLFFALTAHDVLVTGDIGCYSLAVFPPLSRTDTILCMGGG
                     FSVAQGIDKAGEKRRVVGVVGDSTFFHSGITGLLDVVYNKGKSILVVLDNRTTAMTGH
                     QDNPGTGKTLMGEDSDIVSIEAVGKACGMKNVTTVNPYNLVQTKKAIDEAMARDESSL
                     IVTRAPCPLRTRTPVGPIKSIDPSKCTACHSCVKIGCPAIEARKKGEKPVISAELCAG
                     CGLCVQTCKFGAIKEARI"
BASE COUNT          521 a          592 c          551 g          480 t
ORIGIN      
        1 atgcaattat gctggtaatt gtcctccgtt ttatcccaac ctttggagag cggatgcgcc
       61 aggtattgca agccgaggcc gcacgggggt acaatgtgga atccgggaac cccttccgaa
      121 agatcggggt aatactccgg ctgtcgcttc ccttgctcat ttcatgcgcg cgggatgtgg
      181 acaccctggc tctgtcaatc gaaacccgcg gatttgatcc ccgcaatacg accagacctc
      241 ggcgcattcc cccaaccagg ggagacgcgc ttgtcttgat tctttgtttg ggactacaaa
      301 ccgcaattgt gttggcagac cgaatcgttt gcacataagg tgatatccgt atgaaaaccc
      361 tgcttaccgg caacgaagcc gttgcccttg gcgcctggga ggctggcgtt cgcgcagcct
      421 atggttatcc ggggactccg tctaccgaga tactggaaaa tctgtcgcgt cattcaaaag
      481 atgtctatct tgaatggtct ccaaacgaaa aagtagcctt tgaggctgca gccggctcct
      541 cgtatgcggg agtgcgcacc atcgttacca tgaagcacgt tggtctcaat gtggccgcag
      601 accccctgat gaccctatcc tatgttggaa ccgaaggcgg cttccttatt tgtgttgccg
      661 acgatcccgg ccagcactct tcccaaaacg aacaggattc acgccacttt gcccgctttg
      721 ccaagatacc cgtactggag ccggcgaatt ctgacgaggc attacggttt atgaaatatg
      781 gctttgagct atccgaacgt ttcaaaaccc cggttatgat acggcttacc acccgggtaa
      841 gccactcccg aggtctggtg gaaaccggaa accgcatcga atccaaagaa gctcattttg
      901 aacgaaatcc gtcccgcttt tgccccctgc cgatatgggg ccgcgaaatg cgaaagaaag
      961 tagaaatacg cactggcgat cttgcccagg aagcagcgca aagcccgctt aaccggatag
     1021 agatggctga tacatccctt ggtattatcg ccgaaggcct tgcctggaat tatgttaagg
     1081 aagtattccc caccgcttca gttctcaagc ttggctgggc atggccctat cccgactccc
     1141 tgattcggga cttcgcctca caggtacaac gccttgtagt aatcgaggaa aacgaagaca
     1201 ttctggaaga gcatatcaag gctatgggta tagcctgtac gggcagagac cttctttcgg
     1261 gagttggaga gctgtcggtt cccaaactcc tcgctctaaa ggcaaagctc gaaggaaatg
     1321 agcatcccgt cctgcccgct cccatagaag ctgccaagga cctacctgca cgccccccgg
     1381 tcctgtgtcc cggctgcccc catcgcggac tcttttttgc cttaaccgct catgacgttc
     1441 tggttaccgg agacatcggg tgctacagcc ttgcggtatt cccccctctg tcccgaacag
     1501 acactatctt gtgcatgggc ggaggttttt ccgttgccca gggaatcgac aaagccggtg
     1561 aaaaacggcg cgttgtcggc gtagtcgggg actcaacatt cttccattcc ggaattaccg
     1621 gactattgga tgttgtatat aacaagggta aatccattct agtagtgttg gataatcgaa
     1681 ccaccgcaat gaccggacat caggacaatc cgggaaccgg caaaaccctt atgggagaag
     1741 acagcgatat agtatcgata gaggcagtgg gaaaggcctg tggcatgaaa aacgttacca
     1801 cagtaaatcc ctataacctg gtacaaacaa aaaaagcgat cgacgaggct atggcacgcg
     1861 atgagtcctc gctcatcgtt acccgtgcac cgtgtcccct cagaactcgc acccccgttg
     1921 ggccaataaa gtccatagac ccgtccaaat gcaccgcctg tcactcctgc gttaaaattg
     1981 gatgccctgc catagaagcg cgaaagaagg gagagaagcc ggtaatatct gcggagctct
     2041 gcgctggatg cgggctgtgt gtgcaaacct gcaaatttgg agctatcaag gaggcccgca
     2101 tatgagtgaa caaaagccaa caaaaaatat ccttcttgtc ggtg
//
LOCUS       DAOQXQ010000299         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59041_length_2143_cov_3.110632, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000299 DAOQXQ010000000
VERSION     DAOQXQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59041_length_2143_cov_3.110632"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="PKH81_05585"
     CDS             <1..95
                     /locus_tag="PKH81_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ97544.1"
                     /translation="ALLAKENIDGGLIGGAALKAETFKPIALCK"
     gene            209..406
                     /gene="secG"
                     /locus_tag="PKH81_05590"
     CDS             209..406
                     /gene="secG"
                     /locus_tag="PKH81_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ97545.1"
                     /translation="MAIVGIVLLVVFVIICFLIIALVLMQNEEGDGLGGLFAGGSNSA
                     LDPAPEMFLPELPMWWLLSSF"
     gene            382..573
                     /locus_tag="PKH81_05595"
     CDS             382..573
                     /locus_tag="PKH81_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97546.1"
                     /translation="MVVTLFFLTAFFLAFLNRSTGDKGIEEAARIEQGETATEWWSED
                     ATPATTEESASPATTESGN"
     gene            585..1058
                     /locus_tag="PKH81_05600"
     CDS             585..1058
                     /locus_tag="PKH81_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNQ97547.1"
                     /translation="MVLSKVFSPALIKVGLEGEDKEEVFEEMVDLFVASNRSVSRTAL
                     LQAIRQRESKQSTGVMPGIAFPHAQTDEVSSVSGLIGISRAGVDYDAMDGNPVRIVFM
                     MFSSHADCSLHLRALKRLSILLSDPEFSRSLLEQKDSNGVYTSLCRYEDLLTTSV"
     gene            1128..1433
                     /locus_tag="PKH81_05605"
     CDS             1128..1433
                     /locus_tag="PKH81_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97548.1"
                     /translation="MIEQDVIGHLIDVERLAYDLLLDAQTEADKRKNTAKEEAETRFR
                     NAYEELLAHLDAELTRERSVCNESRESDLADFNAHLSSITLDRKAFNDYLDSLYTGS"
     gene            1437..>2143
                     /locus_tag="PKH81_05610"
     CDS             1437..>2143
                     /locus_tag="PKH81_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNQ97549.1"
                     /translation="MDRSSADSYVYAKACGMYSRSYVGPRARKLFEAKRLQDLWSLLY
                     NEEPPLVPEGLLALLLERKTEEQMVQDYITLLSAYDTPDPLSLSFLSLYDYNNLKSAS
                     AQAALGKPERPFMIDTSPFSLFDTDAWPDIGAMTQASPVSWYNRVPSGDDQIDWETKL
                     DHEYYHTLWKSFSSLGKKDRQSVEDLVTDEIILQNIVWALRLKVYYQKSPEEIFPRLA
                     CYDEQAPVSDVLCQAAM"
BASE COUNT          495 a          497 c          528 g          623 t
ORIGIN      
        1 aagccttgct tgccaaggaa aacattgacg gcggacttat cggtggcgcc gctcttaagg
       61 ccgaaacctt taaaccgatc gctctgtgta agtaattagc ccttttttcc cggttctgtg
      121 atgaatggaa ccgggtgttt gtctatgcac ttgcataagg ccttcctttt gggtatagtt
      181 ttgcctgaat tcttaattgg agttatctat ggctatagtc ggtattgtgt tgctggtagt
      241 ttttgtgatt atctgtttcc tcatcatcgc tcttgttctt atgcaaaatg aggaagggga
      301 cggccttggt ggtctttttg ccggcggcag caattcagca ttggatcccg ctccggaaat
      361 gttcttacca gaactaccta tgtggtggtt actctcttct ttttaacggc ctttttcctt
      421 gcctttctta accggagtac cggggataag ggtattgagg aagcggctcg catcgaacag
      481 ggtgaaactg ctactgaatg gtggagtgaa gatgctactc ccgctacaac cgaggaatca
      541 gcttctccgg caaccactga atcgggaaat taagcaggtt tatcatggta ctctcaaaag
      601 ttttctctcc cgcattgatc aaggttggcc ttgagggaga ggataaagaa gaagtatttg
      661 aagagatggt tgatcttttc gtcgcatcga atcggtctgt gtctcgtacc gcgcttcttc
      721 aagcgatacg ccaacgggaa agcaagcaat ctaccggagt tatgcccggt attgcttttc
      781 ctcatgccca gacggatgag gtgtcttctg tttcaggctt gatcgggata tcacgggccg
      841 gggttgatta cgatgcgatg gacggaaatc cagttcgcat cgtgtttatg atgttttctt
      901 cccatgccga ttgttctttg caccttcggg cgctcaaacg gctgtccatc ctgctttctg
      961 atcctgaatt ttctcgctca cttcttgaac aaaaagatag caatggggtg tatacttcgt
     1021 tgtgcagata tgaagatctt ttaacaacct ccgtgtaatc tacggagatt tgacccgatc
     1081 gacgccgagg ctctgctttc ggcgccctgt aactgccaga ggatgatgtg attgaacagg
     1141 acgtaatcgg gcatcttatt gatgtcgaac ggttagccta cgatcttctg cttgacgcgc
     1201 agacagaggc tgacaaaaga aaaaacaccg ctaaggaaga agccgaaacc cgttttcgta
     1261 acgcgtatga ggaacttctt gcacatcttg acgcagaact tacccgggag cgctctgtct
     1321 gtaatgaatc gcgagagagc gatcttgcag atttcaacgc ccatctttcc tcgatcaccc
     1381 tggatcggaa agcctttaac gactatctcg attcattgta taccggtagt taagccatgg
     1441 atcgtagctc cgcggattcg tatgtctatg caaaggcatg cgggatgtat tcgcgctcct
     1501 atgtcggtcc ccgtgcccga aaactctttg aggcaaaacg tctacaagat ttgtggtctc
     1561 tgttgtataa cgaggagcca ccccttgttc ccgaaggctt attagccctt cttcttgaac
     1621 gaaaaaccga agagcagatg gtccaggact atattacgct cttgtccgca tacgacacac
     1681 ccgatcccct gtctctttct tttctgtctt tgtatgatta taacaatctt aagtcggctt
     1741 cagctcaggc tgctcttggc aaaccagagc gtccgtttat gattgacacg agtccttttt
     1801 cgctttttga tacggatgcc tggccggaca ttggggcaat gacccaggca tccccggtat
     1861 cctggtataa tcgggtgcct tccggggacg atcagattga ttgggaaacc aagcttgacc
     1921 atgaatatta tcatacgctg tggaagtctt tttcttcctt ggggaaaaag gatcgtcaat
     1981 cagtagaaga tttagttacg gatgagataa tactgcagaa catcgtttgg gcactcaggc
     2041 tcaaggtgta ttatcaaaaa tcgcctgaag aaattttccc tcggcttgcc tgttatgacg
     2101 agcaagcgcc tgtttcggat gtattatgcc aggctgccat gga
//
LOCUS       DAOQXQ010000300         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59385_length_2135_cov_2.945673, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000300 DAOQXQ010000000
VERSION     DAOQXQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59385_length_2135_cov_2.945673"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKH81_05615"
     CDS             <1..1358
                     /locus_tag="PKH81_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNQ97550.1"
                     /translation="LDDPYSALTIDESFRPWPIASAELEIAYTAYAPASPSGAFVTDL
                     AGNRMKSTPGFLRCLDRTPPKVTISLAGVNRTDLLLLFSKNITGGTGAADGIRLDLAN
                     ADGSITTITPTGISEREEAEQVFIFSLPQAVTASQLVNPLSTIRFITYGNVPNPDTGV
                     PEPASAYSDFLGNYVSLGETHRLTDVGIGLIETLYASDGVNTDGVLGEEEGALRVFDG
                     TGRLLDRNITVGTRIVTGESAPRPLSLYFDVAPPATALPELFNSAAAATSALWLPSVL
                     PGFNKQGNTAARALGAEQILDENRTLRNFVIPEADTEVIPGSRIEMLYRYGDLFCARL
                     ADEADITSLAPWSFSISEMKKQRGGVTILNNVIDAHKREKAIIQVEVPTAGNLVIQVF
                     TLDGNVVRVLERGRKGGGNYTYYWDGTNVGGRPVARGMYFVRVIGPDMDEIRKVMVIK
                     E"
     gene            complement(1366..>2135)
                     /locus_tag="PKH81_05620"
     CDS             complement(1366..>2135)
                     /locus_tag="PKH81_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97551.1"
                     /translation="WTDSGDASHSYDSFSQVTGGGSVRLSSSGGSITYVPVFRTGFSP
                     AQDSVYSIFFRFPPSASAAEFAVYPNAATVYLGSDSIKGSVSGQYSPAVTGESLSLQF
                     TPLEAGTSFGPVFVDDFMLARNEGAILRLRLGRLDTAPQMEGGVYTFSVWVRTDPLAS
                     ATGSPTSYPLDTLIVTMRPVGTGSLASSSARYTYNADNPGWQRLSASLSGKSLQYTLT
                     KEEPNEAILDLVISLENSLPGSIFLAYPALQFHPDGL"
BASE COUNT          566 a          534 c          575 g          460 t
ORIGIN      
        1 gccttgacga tccgtattcg gcccttacga ttgatgaaag cttccgtccc tggccaatag
       61 cctcggccga gcttgagata gcctataccg cctacgcgcc ggcaagccct tcgggcgcct
      121 ttgttaccga ccttgccgga aaccggatga agtccacccc cggattcttg cgctgtcttg
      181 acagaacgcc acctaaagta actatatcgc ttgccggggt taaccggacc gacttgctcc
      241 ttttattctc caagaatatt accggaggaa ccggggctgc cgatggcata cgccttgatc
      301 ttgccaatgc cgacggaagc ataacgacga ttacaccaac cggtatttct gaaagagaag
      361 aagccgaaca ggtttttatt ttctccttac cgcaggcggt aaccgcgagc cagctggtta
      421 atcctctttc tacaatacgc tttattacct atggaaacgt acccaatcct gataccggag
      481 taccagaacc cgcttccgcg tattctgact tcctgggaaa ttacgtgagt ctgggagaaa
      541 cacacagact gaccgatgtg ggtatcggcc ttatcgagac cctctatgcc tctgatggcg
      601 ttaacaccga tggcgtgctg ggagaagaag aaggagcact cagggtcttt gatggaaccg
      661 gtcgccttct tgaccggaac atcaccgtcg gaacacgcat tgtaaccgga gaaagcgcac
      721 cacggccgtt aagtctttat tttgatgtgg cgccccctgc aacagctctg cctgaactgt
      781 ttaattcagc agctgcggct accagcgctt tatggcttcc ctcggtatta cccggtttta
      841 acaagcaagg aaataccgca gcgagggccc ttggagccga gcaaattttg gatgaaaacc
      901 ggacgcttcg caactttgta atcccggaag cggatacaga agttattccc ggatccagaa
      961 tagagatgct ctatcgttac ggggatttat tctgcgcacg acttgcagac gaagcggata
     1021 ttaccagtct tgcaccctgg tcgttcagta tctcggaaat gaaaaaacag agaggtgggg
     1081 taacgatact caataacgtt attgacgctc ataaacggga aaaggcgata attcaggtcg
     1141 aagttcccac tgccggtaac ctggttattc aagtgtttac ccttgatgga aatgtggttc
     1201 gcgtacttga gcgaggccga aagggcggag gaaactacac ctattactgg gacggcacaa
     1261 atgttggcgg cagacccgta gcgcgcggaa tgtactttgt acgggtaatt ggtcccgata
     1321 tggacgaaat ccgtaaagta atggttataa aagaataagc aagggttata acccgtccgg
     1381 atgaaactga agggcgggat aagcaaggaa aatgctgcca ggaagcgagt tttccagact
     1441 gataacgagg tcaaggatgg cctcgtttgg ctcttcttta gtaagggtat actggagcga
     1501 ctttcccgaa agactcgcag aaaggcgttg ccacccggga ttgtcggcat tgtaggtata
     1561 gcgagcagat gagctggcaa gagaacccgt tcccacgggc ctcatggtaa cgatcagcgt
     1621 gtccagagga tacgaggttg gactacccgt agcagatgcc aagggatcgg ttctgaccca
     1681 cacgctaaag gtgtacacgc caccttccat ctgtggagct gtatccaggc ggcccaggcg
     1741 caagcgaagg atagcccctt cgttacgggc gagcataaaa tcatcgacaa atactggccc
     1801 aaaagaggta cccgcttcga gcggagtaaa ctggaggctt aaagactctc cagtgactgc
     1861 ggggctatat tgccctgaaa cagagccctt tattgaatct gaccccaggt acacagtcgc
     1921 agcattggga tagactgcaa actccgcagc agaagcggaa ggggggaaac ggaagaatat
     1981 tgaatagact gaatcctgag caggggaaaa gccggtacga aagacgggaa cataggtaat
     2041 tgagccacca gaagaagaaa gccggacgct tccccctccg gtaacctggg aaaaggaatc
     2101 gtaggaatga gacgcatcac ccgagtctgt ccagg
//
LOCUS       DAOQXQ010000301         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59484_length_2133_cov_3.153994, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000301 DAOQXQ010000000
VERSION     DAOQXQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59484_length_2133_cov_3.153994"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..262
                     /locus_tag="PKH81_05625"
     tRNA            191..262
                     /locus_tag="PKH81_05625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:223..225,aa:Pro,seq:cgg)
     gene            339..1112
                     /locus_tag="PKH81_05630"
     CDS             339..1112
                     /locus_tag="PKH81_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ97552.1"
                     /translation="MHVIPLKILWGDEEYLDRLSMTTDIFYPWLDERPDYPGSSIPDP
                     RRVEQVFLWLSAQYTSIIAIPVGKVLSGTYQVLRTAADHLAAQGYTVSVIDSKLNSAA
                     QGLVVLAAAEDASRGKSHAEILEATAHRIAKSRILVSVASFTYMVRGGRISALKGAIA
                     AATHLKPIITLDPDGRGIAYGASFSQAGSWKKIVREVRRCAKDISRYAVVHAGVPLLA
                     QSYAQELEKILGRECEYIMEISPIVGIHAGVGAVAVAWI"
     gene            1163..1885
                     /locus_tag="PKH81_05635"
     CDS             1163..1885
                     /locus_tag="PKH81_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNQ97553.1"
                     /translation="MDSIFFRLLVSLAAGFLLGLERQMHRQPAGLKTHILICTGSSLL
                     MILSLILAGGINIADLGLLSTIGLVPPSDLSGDPGRLAAQVVSGIGFLGGGAIIRQGF
                     NIKGLTTAATIWVSAAIGLAIGLGAYGAALITLVCVLATLYVMERFESKLFPVKNVKT
                     LYAVYDRADIDITNLEKQLLSLKIAVDTVDMARSMDSGRVKVTFQAHVPPSIDHDALC
                     RLLGAAGPLVRMELTSNEKPSR"
     gene            1866..>2133
                     /locus_tag="PKH81_05640"
     CDS             1866..>2133
                     /locus_tag="PKH81_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNQ97554.1"
                     /translation="MKSRLDKLLADNGLGSRRDIKRLLREGRCVIDGKVCTDAARYVE
                     SEDQSIVLDGKPFVSRSLIYIMLNKPSGVVTSTDDPVHQTVIDLL"
BASE COUNT          462 a          520 c          553 g          598 t
ORIGIN      
        1 cgtagcgaag ctggttatcg cgccggcctg tcaagccgga gatcgcgggt tcaatccccg
       61 tcgctcccgc tagcccgcct tttttaggcg ggtttttttt tgccgcaaca ggatccatta
      121 aaagcggcgt aattaaatcg gatcctgatt atatatatgg gtcgccttct tttgtggaag
      181 acgatcttta cgggcagtag cgcaggggta gcgcgcttgg ttcgggacca agaggtcgtg
      241 ggttcaattc ccacttgccc gagtagcttg tcaactgctc actccttttt ttttctgttc
      301 gctgccattc ttaaaaccga tgtctttacg gatatcacat acacgttatt ccgctcaaga
      361 ttttgtgggg agacgaggaa taccttgatc gtctgtccat gactacggac atcttttatc
      421 catggcttga tgaacgccct gattatccgg gaagttccat ccccgatccc cgccgggttg
      481 aacaggtttt tctgtggctt tcggcgcagt atacttcgat tatcgccatt ccggtgggca
      541 aggtgctcag cgggacctat caggttttgc gtacagccgc agatcatctg gccgcccaag
      601 gctatacagt ctccgtcatt gatagcaagc ttaattctgc ggcacagggc cttgtggtgt
      661 tagccgcggc cgaagacgct tctcgcggga aatcccatgc ggagattctt gaggcgacgg
      721 cacacagaat cgccaagtcg cgtatcctcg ttagtgtcgc ttcctttacc tatatggtaa
      781 gaggagggcg cattagtgct cttaaggggg ccatcgcggc cgctacccat cttaaaccga
      841 tcatcaccct tgatcctgac ggacgtggga tagcttatgg cgcctcattc tctcaagcag
      901 gaagctggaa aaagattgtc agggaagttc gtcggtgtgc aaaagatatt tccagatatg
      961 cagttgtcca tgcaggagtt cccttgttgg ctcaatcata cgcacaggag ctggaaaaaa
     1021 tactgggtcg cgagtgtgaa tatataatgg aaatatcccc gatagtggga atacatgcag
     1081 gagttggcgc ggtagcggtt gcctggatat agaatggtgt cgcacgttgt cagtaaggcc
     1141 cgcgagttgt atactccctg atatggattc tatctttttt aggttacttg tcagtcttgc
     1201 agcaggtttt ctgcttgggc ttgaacggca aatgcatcgg cagcctgccg gcttaaagac
     1261 gcatatttta atatgtaccg gctcttcgct ccttatgatt ctttctctta ttctggccgg
     1321 tggcatcaat attgcggacc tgggtttgtt aagcaccatt ggcctggttc ctccttcgga
     1381 cctttccgga gatccgggcc ggctcgccgc gcaggttgtg tcgggaatcg gttttcttgg
     1441 gggtggggca attatccgcc aaggctttaa cattaaaggt ctgactaccg ctgcgaccat
     1501 ttgggtatct gctgctatcg gattggccat tggtcttggc gcgtatggcg cggctttaat
     1561 caccctggtg tgtgtgttgg caaccttgta cgttatggaa cgttttgaat cgaaactttt
     1621 ccccgtaaag aatgtaaaga ccttatacgc cgtctatgac agagccgaca tagatattac
     1681 caatttggaa aaacaacttc tttcgttaaa aatcgccgtt gacaccgttg atatggctcg
     1741 atctatggac agtggccggg taaaggtaac ctttcaggct catgtaccac cttctataga
     1801 tcatgacgcc ctctgccgcc ttttgggcgc tgccggtcca ctggtacgaa tggaacttac
     1861 ctcgaatgaa aagccgtctc gataagctac tggcagataa tggccttgga tctcgcagag
     1921 acattaaacg acttttaaga gaaggccgct gcgttattga cggcaaggtg tgtacagacg
     1981 ccgctcggta tgttgagtcg gaagaccaat ctattgtatt ggacggaaaa ccgtttgtca
     2041 gtcgctcgtt aatctatatt atgcttaaca aaccctccgg cgtggtaacc tccaccgatg
     2101 atcctgttca ccaaacagtt attgaccttc ttc
//
LOCUS       DAOQXQ010000302         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59488_length_2133_cov_2.918191, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000302 DAOQXQ010000000
VERSION     DAOQXQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59488_length_2133_cov_2.918191"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKH81_05645"
     CDS             <1..248
                     /locus_tag="PKH81_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97555.1"
                     /translation="SNARIALELFASVLDGTSSCESLGDLISNDCLSIARPDGSIAVA
                     SYRRPFPLESALLQEVRTRQEWLQSCGSVDRTDGELI"
     gene            256..1419
                     /locus_tag="PKH81_05650"
     CDS             256..1419
                     /locus_tag="PKH81_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNQ97556.1"
                     /translation="MNLNDKRLGAYRSASNKTDQQAKDDGKRIDSEEKIKKPSSVELP
                     GLIKVATSQDSHGKENPYRKVAKFLILIGVDEAAKVVSRLSPEQTERVALELASIRRV
                     DSDEATVVLAEFESLLRKAREPTGGIDTARSILTQAFGEERADSLLHKAVPFIQGKPF
                     DYLEDVDEDRIYRLITDELPAVKALVLSQMKSKKAAQVINLMGDSEKKETIIRLAKLT
                     GIAPDTVARVDAAMREKIETIDTASSDSIDGRFALAEILRRMDGKGERSILDALSHND
                     QDLARDIRDRLFTLDDIVRADDKFLQEHLRSLGERDIALILAGKSEEIRTKILSAVSK
                     TRGAMILEEEKIIHPVSRQEADTATSVFFLSMRKAWEEGKLFISGRDDRDKWV"
     gene            1422..>2133
                     /locus_tag="PKH81_05655"
     CDS             1422..>2133
                     /locus_tag="PKH81_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNQ97557.1"
                     /translation="MQQAFSHALKAQERLHGFVILSSEPLAEYKGVGIHARHEVTGLE
                     VFHLLTDDEENLFSYSFMTAPEDSTGVAHILEHSVLCGSRRYPLKDPFLVLAKQSVKT
                     FLNAMTFPDKTVYPASSMVEADYFNLMRVYGDAVFFPLLDEWIFRQEGHRFEKASDGS
                     VSIQGVVFNEMKGNYSSFDTIAGDWSLKSILGSTIYAHDSGGDPADIPALTWKQFRDF
                     HRTYYHPVNCKVFLSGNIP"
BASE COUNT          541 a          459 c          535 g          598 t
ORIGIN      
        1 tttcgaatgc ccgaatcgcg ctcgagctgt ttgcttcggt tttggatgga acctcttcat
       61 gcgaatccct tggcgatttg atatcaaatg attgtctttc cattgcgcgc cccgatggca
      121 gtattgccgt tgcctcctat cgccgtcctt ttccgcttga atcggcttta ttgcaagagg
      181 taagaacccg acaagagtgg cttcaatcgt gtggttcagt tgaccgcacc gatggcgagc
      241 tgatatgatg cgatgatgaa tttgaacgat aaacgtcttg gtgcctatcg gagtgcttct
      301 aataaaacag accagcaagc gaaagatgat ggaaagcgca ttgacagtga ggaaaaaata
      361 aaaaaaccat cttctgtcga gcttccgggc cttattaagg tagctacatc tcaggatagt
      421 catggcaagg aaaatcccta tcgaaaggtt gccaaattcc ttattttaat tggtgttgat
      481 gaagcggcca aggttgtctc tcggctaagt cctgagcaaa cggagcgggt tgcccttgag
      541 cttgcctcaa ttcgcagagt ggatagcgat gaagcgactg ttgttctggc tgaatttgag
      601 tccttattgc gaaaagcccg ggagcccacc ggcggaatag atacagcccg gtcaatatta
      661 acccaggcct ttggcgaaga gcgggccgat tcgcttttgc ataaggctgt cccgtttata
      721 cagggaaaac catttgacta ccttgaggat gtggacgagg atcgcatcta tcgtcttatt
      781 accgatgagt tgcctgctgt aaaggccttg gttctttccc agatgaaaag caaaaaagca
      841 gctcaggtaa taaatctgat gggcgacagt gaaaaaaaag aaacgatcat tcgtttggca
      901 aaactaactg gcattgctcc tgatacagtt gcccgtgtgg atgcggcaat gcgcgaaaaa
      961 attgaaacga tcgatacagc ctcttctgat tcaattgacg gtcgttttgc cctggcagaa
     1021 attctgcgac gtatggatgg aaagggcgag cggagtattc ttgatgccct ctctcataat
     1081 gatcaggatt tggcccggga tatacgcgat agactattta ccctggatga catcgtcagg
     1141 gccgacgata aattccttca ggagcacctt cgctctcttg gcgaacgaga tatcgccttg
     1201 atacttgcag gtaagagcga ggaaatccgc acgaaaattc tctctgcggt gtccaagacg
     1261 cgcggtgcaa tgattttgga agaagagaaa attattcatc ctgtttcacg gcaggaagcc
     1321 gatacagcta catccgtttt tttcctttct atgcgtaagg cgtgggagga gggaaaatta
     1381 tttatttcag gtcgcgacga tcgcgataag tgggtataac tatgcaacaa gccttttcgc
     1441 acgcgcttaa ggcgcaagag cgccttcatg gatttgttat tctttcttca gagccattgg
     1501 cggagtataa gggtgttggt atccatgccc gacatgaggt aacaggcctt gaggtcttcc
     1561 atctgctaac tgatgatgaa gaaaatcttt tttcttattc gtttatgacg gcaccggaag
     1621 attctaccgg ggtagctcat attcttgaac attcagtcct ttgtggttct cgccgttatc
     1681 cgctcaaaga cccgtttttg gtattggcaa agcaaagtgt aaaaaccttt ttgaatgcaa
     1741 tgacctttcc tgataagaca gtgtatccgg caagctccat ggtcgaggcc gattatttta
     1801 atctgatgcg agtatatggg gatgcggttt tttttcccct gctcgatgag tggatattcc
     1861 gtcaggaagg tcatcgtttt gaaaaagcga gtgatggctc tgtttcaatt cagggcgtag
     1921 tgtttaacga gatgaaaggt aactattcat cctttgatac aatagctggt gactggtcct
     1981 tgaagtcaat tcttggctca accatctatg cccatgactc tgggggtgat cctgccgata
     2041 ttcctgcgtt aacatggaag cagttcaggg attttcatag aacctactat catccggtaa
     2101 actgtaaggt ctttctttcg ggaaatattc cca
//
LOCUS       DAOQXQ010000303         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59507_length_2133_cov_2.018768, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000303 DAOQXQ010000000
VERSION     DAOQXQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59507_length_2133_cov_2.018768"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2133)
                     /locus_tag="PKH81_05660"
     CDS             complement(<1..>2133)
                     /locus_tag="PKH81_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNQ97558.1"
                     /translation="TETDIRTKYIIPAIKGAGWDITTQVREEVYFTDGMINVKGHLST
                     RGKRRRADIILYYKPNIPIAVIEAKDNNYTVGAGIQQALGYADTLDIPVAYSSNGDGF
                     LEHDRSNFLGTIERELLLENFPSPEELWKRYKKYRGIETDEQDRIASLDYFYDSAGRT
                     PRYYQQIAINRTVEAISKGQNRILLVMATGTGKTYTAFQIIYRLWKSGKVERVLFLAD
                     RNVLIGQAKRNDFKHFKEKMTIIRKKQIDKAFEIYLALYQGLTNYNDDKDAYKEFSRD
                     FFDLVVVDECHRGSAAADSAWREILTYFNSAIHIGLTATPKETKEVSNIEYFGDPVYT
                     YSLKQGIEDGFLAPYKVIRVGLNVDLEGWRPEIGKTDKDGNLVEDRTYNTKDFDRNLV
                     IDERTQTVARKVTEFLKVTDPFDKTIVFCVDIEHAERMRQALAIENSELVKDNYKYVM
                     RITGDDEEGKRELDNFINPEERYPVIATTSKLMTTGVDAQTCKLIVLDSVIKSMTEFK
                     QIIGRGTRINEEYGKTFFTIMDFRNVTDLFADTAFDGEAVMIKEIKGEEEITKDDVET
                     GEDEEHPEEADGAGDDGKEKIQITTIVDGGPITTEPRPKVYVAGVDVSILNERVQYMD
                     QNGKLITGSLKDYTKQNILKEFRTIDDFLTRWNKTDKKKALIDELEENGILLANLKEE
                     VKKDLDLFDLICHIAWDMPALTRKERAE"
BASE COUNT          491 a          502 c          432 g          708 t
ORIGIN      
        1 tctcggccct ttctttcctg gtaagggccg gcatatccca ggctatatgg cagatgagat
       61 cgaagagatc gaggtctttc tttacctctt ctttcagatt tgcaaggaga ataccgtttt
      121 cttccagctc atctataagt gctttctttt tatctgtttt attccatctc gtaaggaagt
      181 catcgatagt gcggaactcc ttcaggatat tctgcttcgt atagtccttt aaacttcctg
      241 tgatgagctt gccgttctgg tccatatatt ggacgcgttc gttaaggata gagacatcga
      301 caccggcaac ataaaccttc gggcgtggct cggtcgttat agggccgcca tcaacaattg
      361 ttgttatttg tattttttct tttccatcat ctccagcgcc atcagcttct tcggggtgtt
      421 cttcatcttc accggtttcc acatcatctt tggttatctc ttcttcccct ttgatctctt
      481 taatcatgac cgcctcaccg tcaaaagcag tatcagcaaa taggtcagtc acgtttcgga
      541 agtccataat agtgaagaaa gtctttccgt attcttcgtt gatacgcgtt cctctaccga
      601 tgatctgttt gaactctgtc atcgatttga taacgctatc aaggacgatc agtttacagg
      661 tctgtgcatc aaccccagtg gtcatcaact tcgatgtggt tgcgattacc ggataccttt
      721 cttctggatt gatgaaatta tctaattccc gttttccttc ttcatcatct ccagtgatac
      781 gcataacata tttatagttg tccttcacga gctcactatt ttcgatagca agagcctggc
      841 gcatacgttc agcatgttct atatccacac agaatacaat cgtcttatca aaaggatctg
      901 tcaccttgag aaactccgtt acctttctgg ctacggtctg tgtgcgttcg tcgatgacga
      961 ggtttctatc aaaatccttg gtattgtatg tgcgatcctc aacgagattg ccatccttgt
     1021 cagttttccc tatctccggt ctccatccct caagatcgac attaaggcca acgcggatta
     1081 ctttgtatgg tgcaagaaag ccgtcctcaa taccctgttt tagcgagtag gtatagaccg
     1141 ggtcaccaaa atattcaatg ttcgatactt cttttgtttc tttcggggta gctgtcaaac
     1201 cgatatggat ggctgaatta aagtatgtaa ggatctcgcg ccacgcacta tccgctgctg
     1261 cacttcccct atgacactca tcaacaacaa ccagatcaaa gaagtcacgg ctaaactctt
     1321 tatatgcgtc cttatcgtca ttgtagttcg ttaatccctg atagagggcc agatagatct
     1381 cgaaggcttt atctatctgt ttttttcgga taattgtcat tttctctttg aaatgtttga
     1441 aatcattcct ttttgcctgg cctattaaaa cattcctatc ggcgaggaat aaaacccttt
     1501 ccaccttacc gctcttccaa agccggtaga taatctgaaa ggccgtgtat gttttgcccg
     1561 ttcctgtagc cattacaagg agtattctat tttgtccctt tgagatggcc tcaacggtcc
     1621 tattaatggc aatctgttga tagtaacgtg gtgttctgcc agcgctgtcg tagaaatagt
     1681 caagtgacgc gatgcggtct tgctcgtctg tttcaatgcc tctatatttt ttgtatcgtt
     1741 tccagagttc ttcaggggat gggaagtttt ctaataaaag ttcccgttcg atcgttccca
     1801 aaaaattgga tctgtcgtgt tcgagaaaac catcgccatt tgagctgtat gccacaggga
     1861 tatcgagtgt atctgcatag cccaaggctt gttgaatgcc agcaccaact gtgtagttgt
     1921 tgtctttggc ttcgataaca gcaataggga tattcggttt atagtacagg attatatcag
     1981 cccttcttct cttgccacgc gtggaaagat gtccctttac attgatcatg ccatcagtaa
     2041 agtagacctc ttcacggacc tgagtggtaa tgtcccatcc tgctcccttg atcgcaggga
     2101 tgatgtattt tgttcggatg tctgtttcag tta
//
LOCUS       DAOQXQ010000304         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59595_length_2131_cov_2.450867, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000304 DAOQXQ010000000
VERSION     DAOQXQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59595_length_2131_cov_2.450867"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="rsxC"
                     /locus_tag="PKH81_05665"
     CDS             <1..1212
                     /gene="rsxC"
                     /locus_tag="PKH81_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNQ97559.1"
                     /translation="QRIAEGSAFMSVPVHASVSGTVKKIESWLSTGNVEVPCIAIEAD
                     GSSSVSFMSPLDPFTCTKEESLARVRDAGIVGMGGAAFPAHVKLSPPKGSKIDYLIAN
                     GAECEPYLTIDDRTMRESAQKVVDGIAISMHLVGASRGIIALEDNKAELVPILEKAIQ
                     AQKSNPVASGAYDISITLCKTKYPQGGEKMIIKAVVGREVPSGGLPSQAGCVVQNVGT
                     LKAISEAFREGKPLIDRPLTISGGACATPKNIEVPIGTLVGDLIPATVNLNPGVVKIL
                     SGGPMMGFSMKSAAFPVLKNTSGVLFLTAAETSVEEESACIGCGRCIKVCSCRLTPVL
                     IARAIKGGNLDAAKRCGLMDCVECGTCASVCPARIKLVQHFRVGKAAVRAAAQKEAER
                     AARKEASQGGR"
     gene            1212..>2131
                     /locus_tag="PKH81_05670"
     CDS             1212..>2131
                     /locus_tag="PKH81_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ97560.1"
                     /translation="MAESDRNEIYLSSSPHYTIPVTTRHVMAMVILALLPIAVWGVVL
                     FGLPALITLITAIVSCVGFEALFRKMTGQDVRAGDLSAVITGLLFAMVLPPTTPVWMT
                     ILGSLFAIVVAKEFFGGLGANVFNPALAGRAFLMVSFAKHMTNWVENRFTAVMDAVST
                     ATPLALIKAADATNAEALAGELGFFSSADTYWNLFIGNRGGCIGESSIFLILFAFFFL
                     SLTKVIDWRAPVAMMATTVAITWIAGIDPLLTLLSGGLAFGAVFMTTDYATSPVTDTG
                     RLLFGAGCGLITALIRVFGGFPEGVMFSIL"
BASE COUNT          433 a          548 c          587 g          563 t
ORIGIN      
        1 cagagaattg ccgaaggatc agcgtttatg tctgttcccg ttcacgcatc ggtgagcgga
       61 acggtaaaaa agattgaaag ctggctttcc accggtaatg ttgaggtacc ctgtatcgcg
      121 attgaggccg acggatcatc gtctgtttcc tttatgagtc cccttgatcc gtttacctgt
      181 accaaggaag aatcactggc ccgcgtccgt gatgcgggta ttgtgggtat gggcggggct
      241 gcctttccgg cacatgttaa gctcagtccc cccaagggat ccaaaattga ctatttaatc
      301 gcaaacgggg ccgaatgtga gccctatctg acgattgacg accggaccat gcgcgagagt
      361 gcgcagaagg ttgtcgatgg tatagctatt tccatgcatc tggttggggc ctctcgggga
      421 attattgccc tggaggacaa caaggccgag cttgtgccca ttctggaaaa ggcaattcaa
      481 gcccaaaaaa gtaatccagt ggcatccggg gcttatgata tcagtattac cctttgtaag
      541 accaagtatc cacagggtgg agagaagatg attatcaagg cggtcgtggg cagggaagtg
      601 ccttcgggag ggcttccctc tcaggctggt tgtgttgttc aaaacgtggg aacccttaag
      661 gcgatttcgg aagctttccg cgagggaaag ccgcttattg accgtccgct tacgatttcc
      721 ggcggcgcct gtgccactcc aaagaacata gaggtaccta taggaaccct tgtcggtgat
      781 ttgattcctg ctacggtaaa ccttaatccc ggtgtggtaa aaattctctc cggcggaccg
      841 atgatgggct tttccatgaa aagcgctgcc ttccccgtgc tcaagaacac ttccggcgtc
      901 ctttttttga ctgctgccga aacctctgtc gaagaagaga gtgcctgcat tggctgcggc
      961 cgctgcatta aggtctgtag ttgccgcctt accccggtgt taattgcccg cgccataaag
     1021 ggcggaaatc ttgacgcggc gaagcgctgt ggcttgatgg attgcgtgga gtgtggcacc
     1081 tgcgcttcgg tatgtcctgc tcgcatcaag ctcgtgcaac atttccgtgt tggcaaggct
     1141 gccgttcgcg ccgccgctca aaaggaagcc gaacgggcag cgcgtaaaga agcatcccaa
     1201 ggaggccggt aatggctgaa tcagacagaa acgaaatata tctttcatca agcccccact
     1261 acacaattcc ggttacaacc cgtcatgtta tggcaatggt gattcttgcc ctgcttccca
     1321 tagcggtgtg gggcgttgtc ctttttggtc ttccggccct gataaccctg attaccgcga
     1381 ttgtatcctg cgttggcttt gaggccctgt ttcgcaagat gacaggccaa gatgttcgcg
     1441 cgggcgacct gtcggcggta attaccggtc tcttgtttgc catggtcctg cctccaacaa
     1501 cgccggtatg gatgaccatt ttgggatcct tgtttgcaat cgtcgttgcc aaggagttct
     1561 ttggcggtct gggcgcaaac gtgtttaacc ccgccttggc cgggcgagct tttttgatgg
     1621 tgagctttgc aaagcacatg actaattggg tggaaaaccg ctttaccgcg gttatggacg
     1681 cagtctctac cgcaaccccc cttgccctta taaaggctgc cgacgcaacc aacgcggagg
     1741 ccttggccgg cgagctcgga ttcttctcca gtgcggatac ctattggaat ctttttattg
     1801 gaaaccgtgg cggctgtatt ggcgaatcct ccatttttct gattcttttt gcctttttct
     1861 ttttgtccct taccaaggtg atagattggc gagctccggt tgcaatgatg gcaacaaccg
     1921 tggcaataac ctggatcgcc ggcattgatc ccctgttaac cctgctttcc ggcggtttgg
     1981 cctttggcgc ggtctttatg actaccgatt atgccaccag tcccgtaacc gatacagggc
     2041 gtcttctttt tggcgccgga tgcggtctta ttaccgccct gattcgtgtg tttggtggat
     2101 tcccggaggg agttatgttc agcatcctga t
//
LOCUS       DAOQXQ010000305         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59711_length_2128_cov_3.747709, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000305 DAOQXQ010000000
VERSION     DAOQXQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59711_length_2128_cov_3.747709"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKH81_05675"
     CDS             complement(<1..370)
                     /locus_tag="PKH81_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97561.1"
                     /translation="MKKRSGLCAVLGVLVIAAFFANDRRAIEEGIALHNAAEVTPEGN
                     IERGKAILAPLIDKYPIAKGYYGSLITLEAGVWEKNKNAIKATTCLENGTALIDEAVA
                     AGPEISDLRFLRMINSYELSV"
     gene            complement(389..1639)
                     /locus_tag="PKH81_05680"
     CDS             complement(389..1639)
                     /locus_tag="PKH81_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97562.1"
                     /translation="MKRLLLCVVVACCSLSFYSESSPDILVGKIRIFIDQAEYLWTQS
                     ALERLDDQVISPPIQVETVISFLSIQPGRYTSEEDVASEAEVSQRRLRETGYFYEASI
                     MIIPPKKVLHERTLVINVTTGFLTRFGGGGIWAMYGKVAMGGRRDALYLYAGYNKSGI
                     RYLQSHVGGSPVILGGSLFYVGPGDYPGKSDGQDVENRFAASITTGWAITPDLSIGIE
                     PTVEGFGFSSGGLLSVQPFISQRILVQTGHQSEGGVNLRLFWYPEHTGVKGEGSVYAR
                     LGITKRLTFAVKGSGGYSPTKIPEEALFDLYNTEDANVRSGYSQQELRAADYILASAE
                     VRFTVLETYIPPILNCVVQPFVFVDASQTLSSTKVGDKDFFDAYGLGVRVLLNNPVFA
                     YFTFSYGVNNEGDGRFLFCGTAGY"
     gene            complement(1734..>2128)
                     /locus_tag="PKH81_05685"
     CDS             complement(1734..>2128)
                     /locus_tag="PKH81_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97563.1"
                     /translation="GGESPEERMVGIGSVLHEVASLHASDCIDLVVDLVSPSLSRYET
                     HLEGLLEEYNRQPDQWAQDVEALLGNLREKIREPALLFGPGGCGLGLSAFQEELAAYG
                     ALLEAWPEVWAHCVRHNADGKPFLASGA"
BASE COUNT          566 a          570 c          466 g          526 t
ORIGIN      
        1 taacagaaag ttcataacta ttaatcatgc gaaggaatct tagatcgctt atctccggtc
       61 ctgctgcaac tgcctcgtcg atcagggcag tgccgttttc cagacaggtt gttgccttta
      121 ttgcgttttt atttttttcc cacacgcctg cttccagagt tatcaagctt ccgtaatatc
      181 cttttgcgat cgggtacttg tcaatcaacg gggcgaggat cgctttcccc ctttcaatat
      241 ttccttcggg agtaacttcc gccgcattat gaagggcaat accctcctct atcgcacgac
      301 gatcatttgc aaaaaaagcc gcaattacca acacaccaag aacagcacac aatccacttc
      361 gtttcttcat agtctttgtc tccttatctt aatagccggc ggttccacaa aagagaaacc
      421 tgccatctcc ttcgttatta acgccatagg aaaaagtgaa atacgcgaac acggggttat
      481 tgagaaggac gcgcacgccc aggccatagg catcaaagaa atccttatcc cccaccttgg
      541 tggagctgag cgtttgagaa gcgtccacaa agacaaaggg ttgaacaaca cagttcagta
      601 ttggcggtat atatgtttcc aatacggtaa accgaacctc cgccgaggca aggatatagt
      661 cggcggcacg aagttcttgc tgcgaatacc ccgagcgcac attcgcatct tccgtgttgt
      721 acaaatcaaa cagcgcctcc tcggggattt tggttggcga gtatccacca cttcccttaa
      781 ccgcaaaggt taatcgtttg gtaataccga gtcgtgcgta cacactgcct tctcccttta
      841 ccccggtatg ctccggatac caaaagagtc gaaggttaac gccgccttcg ctttgatggc
      901 ctgtttgaac taatattcgt tggctaataa agggctgaac ggagagcaat cccccggagg
      961 aaaaaccaaa accttccacc gtcggttcaa ttccgattga aaggtccggt gttatcgccc
     1021 atccagtggt aatagaggca gcaaagcggt tttccacatc ctgaccatca ctctttcccg
     1081 ggtagtctcc aggaccaaca taaaacagag agccaccaag gataaccgga gaaccgccta
     1141 catgtgactg taggtatcga atcccgctct tgttatatcc ggcatacaga tagagcgcgt
     1201 cccgccttcc acccatggcc accttgccgt acatggccca gatgccgccg ccgccaaaac
     1261 gggttaaaaa gccggttgtc acattgataa ccagggttcg ctcatgtaaa acctttttag
     1321 ggggaataat cataattgac gcttcataaa aataaccggt ttcgcgaagc cgacgctgtg
     1381 agacttctgc ctccgaagca acatcctctt cagatgtgta tcgacccggt tgtatagaaa
     1441 gaaacgatat aacggtttcg acttgtattg gaggagaaat cacttgatcg tccagtctct
     1501 ccagagcact ttgagtccac aggtattccg cctgatctat aaaaatgcgg attttcccaa
     1561 ccaggatatc aggggatgac tcgctataaa aagacagaga acaacaagct actacgacgc
     1621 acagcaataa tctcttcaca ttctacctca tactactatt acacgcatag tagtatgata
     1681 aaacatgaac tgtcaatgac ataaatgttg ttttttactt tttttgtgtg ttatcaggcc
     1741 ccagaggcca aaaaaggctt tccgtcagca ttgtggcgta cgcaatgtgc ccacacctcg
     1801 ggccaggcct ccaacaaggc gccataggca gcaagctctt cctggaaggc cgaaagccca
     1861 agcccacagc cgcctggccc aaaaaggagg gcaggttccc ttattttttc cctcagatta
     1921 ccaagtaggg cttctacatc ctgtgcccac tggtcgggtt ggcggttata ttcttccaaa
     1981 agtccttcaa gatgcgtttc gtaacgggag agacttggtg aaacaaggtc taccaccaag
     2041 tcaatgcaat cggaggcatg aagggaggcg acttcgtgta atactgatcc aatgccgacc
     2101 atccgctctt cggggctttc ccctcccg
//
LOCUS       DAOQXQ010000306         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59717_length_2128_cov_3.504100, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000306 DAOQXQ010000000
VERSION     DAOQXQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59717_length_2128_cov_3.504100"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1413
                     /gene="metK"
                     /locus_tag="PKH81_05690"
     CDS             244..1413
                     /gene="metK"
                     /locus_tag="PKH81_05690"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNQ97564.1"
                     /translation="MKSKRHLFTSESVGEGHPDKLCDQISDAILDACLREDPTSHVAC
                     ETFASTALVLVGGEITTSTYVDVQQVARTIARDIGYTDSDYGLDCDSMAVLDMIHSQS
                     PDINQGVVGSGLDEYKGQQGAGDQGMMFGFACTETPQLMPAPIMYAHDLLHKATALRK
                     SRIIPWLRPDAKSQVTIEYEGHKPLRIDTVVISHQHDPEIDHNTIKETIIDQVVKPIL
                     SPTGLLDAQTKYFINPTGRFVVGGPFGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPS
                     KVDRSGAYMARYVAKNLVAAGLAERCEVQLAYAIGVPFPVSVMVETFGTGKVEEAALV
                     KAVTEVFDLTPAGIIKTLDLCRPIYQATASYGHFGRDMFPWEQTDKTELLTRAIR"
     gene            1458..>2128
                     /locus_tag="PKH81_05695"
     CDS             1458..>2128
                     /locus_tag="PKH81_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ97565.1"
                     /translation="MASRRVTIIVPQESRYRLIDVLNATLNNRLASLSLPSLSKSKIR
                     RLIPAGTVRVDNKTVTVPALLVRPGASITIDIDDSRAFSDKDPGDITFELDASSIIYE
                     DDAIIAVNKPPRFPSEATVVEGRDHLLAAVKRYLHRQTASRNVPYAGLLHRLDRETSG
                     IIILTKTRDANKSLFTQFSEHTIQKEYIALSWWNSTLPPPDEFTVHDYLSRISSKSAQ
                     AKWGPV"
BASE COUNT          569 a          563 c          448 g          548 t
ORIGIN      
        1 ccttccttgt tgcgggaaag actatgacag gatggatccc agttattaaa atcaccagcc
       61 aaagaaacct tggagacgcc taccgtagcc tctacgggca attcaaaggt taccttgcag
      121 gtttttccct ttttggggta tgccttatat aaagccatct aacgctcctt ctggttttta
      181 ttattgcttt cttgtatagt aaaatatact agtatttatt ccacattgca agggagatac
      241 catatgaaat caaaacgaca cctttttaca tcagagtcag tcggcgaagg ccatcccgac
      301 aagctttgcg atcaaatatc agacgctatt cttgatgcct gtttacgcga agacccaacg
      361 agtcatgttg cctgcgagac ctttgcctct acggctcttg ttcttgttgg aggagaaatc
      421 actacatcaa cctatgttga cgttcaacaa gtagcccgaa ctattgcgcg ggatatcggc
      481 tatacggatt cagattatgg cctggattgt gactctatgg ctgtgcttga tatgatccat
      541 tcacagtcgc ccgacatcaa tcagggcgtt gttggatccg ggttggacga atacaagggg
      601 caacaaggcg cgggagacca gggcatgatg ttcggttttg cttgtaccga aactccccaa
      661 ctcatgccag cccccatcat gtacgcacat gatcttttac ataaagccac tgcactacga
      721 aaatcccgta ttattccctg gttacgcccc gatgcaaaaa gccaggttac gattgaatac
      781 gaagggcaca aacctctccg gatagacacc gtagttatct cccatcaaca cgatccagag
      841 attgatcata ataccatcaa agaaaccatc attgaccaag tggttaaacc aatcctttcc
      901 cctaccggtc ttctggacgc tcaaacaaag tattttatca atcccaccgg tcgctttgtt
      961 gtaggcggcc cctttggcga cactggatta acgggccgaa agattatcgt ggacacctat
     1021 ggcggtatgg gacgccatgg aggaggagcc ttttccggca aagatccctc caaagtagat
     1081 cgatctggcg cgtacatggc ccggtatgta gctaaaaatc ttgttgccgc aggtctggca
     1141 gaacgttgcg aagtccagct tgcctatgcc ataggcgtac ccttccctgt ttcggtaatg
     1201 gtcgaaacct ttggcacagg aaaggttgaa gaagcggcac tggtaaaagc tgtaacggaa
     1261 gttttcgatt taacaccagc cggcatcatc aaaacccttg atctgtgtag acctatctat
     1321 caagcaacag cgtcgtatgg acactttggc cgagatatgt tcccctggga acaaacagac
     1381 aaaaccgaac tccttacccg cgccattcga taacaagata tcgttgatct aaacgccgcg
     1441 agctggtacc atacgccatg gccagccgcc gcgttaccat tatcgttccc caagaatctc
     1501 gttaccgact aatagacgta ctcaatgcaa cgcttaataa tcgtcttgcc tctctatcgc
     1561 ttccatctct ttccaaatct aaaatccgcc gacttatccc ggccggcacg gttcgggttg
     1621 ataacaagac agttacagtt cctgctctct tggtccgtcc cggagccagt atcactatag
     1681 atatcgatga ttctcgcgcc ttttcagaca aagaccctgg agatattacc tttgaacttg
     1741 atgcatcctc catcatctat gaagatgatg caatcattgc agtaaacaag cctcctcgat
     1801 tcccctcaga agcaactgtt gttgaaggtc gcgatcatct tctggctgcg gtaaaacgct
     1861 acctgcatcg tcaaactgct tccagaaacg tcccctatgc cggcttgctt catcgccttg
     1921 atagagaaac ctccggaata atcattctta ctaaaacaag agacgcaaat aaatcacttt
     1981 ttacccagtt ttcggaacat accatccaaa aagaatatat tgccctctca tggtggaatt
     2041 caacattgcc cccgcctgac gaattcacgg tccacgacta tctgagtcgc atttcaagta
     2101 aaagcgcgca agccaaatgg ggccctgt
//
LOCUS       DAOQXQ010000307         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_59992_length_2122_cov_2.560232, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000307 DAOQXQ010000000
VERSION     DAOQXQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59992_length_2122_cov_2.560232"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKH81_05700"
     CDS             complement(<1..619)
                     /locus_tag="PKH81_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97566.1"
                     /translation="MVEPSGFYTQLASRSGFNASVRHINRTLDLLDSCGTKSNIADEI
                     TALMADKSFDEDEILAAVNMLLIEKWGYSVVSYNITSVVSDALPLVQEVARWKAVDLV
                     LGYHHPDLGFIVVNPKNPGSASVISGFRKNELMVVYALGSGTTESSRKNAEEAITLLI
                     KLVEGSSITASRALLNGPFMFKPPKKIQPKKAQAPKSRSVASRSAA"
     gene            complement(739..>2122)
                     /gene="cfpA"
                     /locus_tag="PKH81_05705"
     CDS             complement(739..>2122)
                     /gene="cfpA"
                     /locus_tag="PKH81_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HNQ97567.1"
                     /translation="DLIAKHYMDEIDKAIEKLKDDRIDQGLDELSDGEIIFAKMRKVK
                     DLTDDDVDNPKSRRYEIMAKEMMDRINNLRAEIDPATFDQLNVRENLKKIIDTENIRN
                     RGFNTAINSLTSILDTSKMGYQYIENLKNARELILREYDDTNAAVLPDERYQIRLRYY
                     DQQQLLEDRKAYEIMIRSFETEVEHLWDVLYTRYEDSKFLTRVTDFNDLAKIYKKKIR
                     KNIKEKTGDPLYEDIAKVWDEISFVKTGETDVEKMNRTFLYEKDKIRKRLILMRDKLK
                     HMYDYQYPIERRVMEERLSFLETEFMKFDYIINPFHLQPGLLLDVDITSIKRKKATLD
                     GMANVLNEFLHGVSKGFQDAAFASFSRRRSTVRADIAQTFATDHGDDPVAGEPPARAA
                     YLDMLNDEPASAPAAIAAPAPSRAKPKAAPAKRAKAPAKSAKSGVVDSGRGRRGRATA
                     SKGRKGLREI"
BASE COUNT          545 a          476 c          498 g          603 t
ORIGIN      
        1 aagcagcaga acgagaagcg acagaacggg atttaggagc ctgtgctttc ttgggctgaa
       61 tctttttagg tggtttaaac ataaacggtc cgtttaacaa ggcacgagaa gcagtaatcg
      121 aagagccttc tacgagcttg ataagaagtg ttattgcctc ctctgcattt ttgcgagagg
      181 actcagtagt acccgaacca agggcataca caaccatcaa ctcgttcttt ctaaatccag
      241 aaataaccga agcacttcct ggattcttgg gattaacaac aataaatccc agatctggat
      301 ggtgatatcc aagaactaga tctactgcct tccagcgtgc tacttcctga acgagaggaa
      361 gtgcatcaga tactactgaa gtaatattat aagaaacgac ggaatacccc catttctcta
      421 tcagaagcat atttaccgct gcaagaattt catcttcatc aaaggattta tctgccatga
      481 gcgcagtaat ctcatctgcg atattactct ttgttccaca ggaatcgagc aaatcaagtg
      541 ttctattgat atgtcgtaca gacgcgttaa acccggagcg ggacgcgagt tgtgtataaa
      601 aaccggacgg ttctaccatt ttgtcctcct catttcgtaa cagtataccc cataattgac
      661 ataaccgata aaaaaaaacc cccgaaacac agtaattttc cgttttcggg gggtagtggt
      721 tacatcatat atgacctatt agatctctct caggcccttt ctgcccttag aagcggtagc
      781 tcttcctctg cgtccgcgtc cggaatctac aacaccggat ttagcactct tggcaggagc
      841 ttttgcccgc tttgcgggtg ctgctttcgg ctttgcccgt gaaggagcag gagctgcaat
      901 tgcagcggga gccgaagcgg gctcgtcatt gagcatatca agatatgccg cacgagcagg
      961 cggttcgccg gctacaggat catcgccatg atcggttgca aatgtctgtg caatatcggc
     1021 acgaacagtt gaacgacgtc ggctaaagga ggcgaatgct gcgtcctgga atcccttgga
     1081 tacaccatgg aggaattcat taagaacgtt tgccattcca tcgagtgtag ctttctttcg
     1141 cttgatagaa gtaatatcaa cgtctaacaa gagtccgggc tgcaggtgga aaggattgat
     1201 aatgtaatcg aacttcatga attcagtttc caggaaagag agtcgctctt ccataacgcg
     1261 gcgctcaatg gggtactgat aatcgtacat gtgtttgagc ttgtcgcgca tcaggataag
     1321 gcgtttgcgg atcttgtctt tttcgtacaa gaaggtccgg ttcatctttt ctacgtctgt
     1381 ttcgccggtc tttacgaaag aaatttcgtc ccataccttg gcaatgtctt catacaaagg
     1441 atcgccggtc ttttctttga tgttcttgcg gatttttttc ttgtaaatct tggcaagatc
     1501 gttaaagtcg gtaacacggg taaggaactt ggagtcctca tagcgggtgt acagaacatc
     1561 ccacagatgc tcaacttcgg tctcgaaaga acgaatcatg atctcatatg ccttgcgatc
     1621 ttcgagcaac tgctgttgat cgtaatagcg cagacgaatc tgatagcgtt cgtccggaag
     1681 aactgcggca ttagtgtcgt catattcgcg aaggatgagt tcgcgggcat tcttaaggtt
     1741 ttcgatgtac tgatagccca tcttggaggt atcgagaatc gaggttaaag agttaattgc
     1801 ggtgttaaat ccgcggtttc ttatgttctc ggtatcgata atcttcttga ggttttcgcg
     1861 aacattgagc tgatcaaagg ttgccggatc gatttctgcg cgaaggttat tgatgcgatc
     1921 catcatttcc ttggccataa tctcgtagcg gcgacttttg ggattatcta cgtcgtcatc
     1981 ggtaaggtct ttaacttttc gcatcttggc aaaaataatc tcgccatcag aaagctcatc
     2041 aagaccctga tcgatgcggt catccttgag tttctcgatt gccttgtcga tttcgtccat
     2101 gtagtgcttt gcaataagat cc
//
LOCUS       DAOQXQ010000308         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_60608_length_2108_cov_3.056990, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000308 DAOQXQ010000000
VERSION     DAOQXQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60608_length_2108_cov_3.056990"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1316
                     /locus_tag="PKH81_05710"
     CDS             42..1316
                     /locus_tag="PKH81_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ97568.1"
                     /translation="MKSQNILHEILDSLKTMVWFVILLFPLVVMKVSVSSGVASVSFR
                     WAYIPLTALGSFVLSFIWNRALQWSENRASMGNANLQKGLVYRTSEIFRQKKTRTSAL
                     IILAILVLAYPHALGMYHTNIMITALIYVILALGLNIVVGLGGLLNLGYAAFFAVGAY
                     TYGLLWKYAGPVFTEAGIHTGWLFWIALPLAGLIAALFGILLSLPVLRLRGDYLAIIT
                     LAFGEIIRMFLQNSGDLTGGAAGISLIPRPWFFAMKLRPPAAALYIYYIVVALVLLTI
                     LVVRRIEDSRVGRALEAMKEDEIACEAMGIDLVKNKLITFSLGAFWAGIAGVVLAAQT
                     TFINPDSFTLWESIMILMAVVIGGTGSIPGTIAGALILKLLPEYFRALSQYRMLIYGV
                     AMIVIIIFKPEGLIPRNRKKYTISTVDEKEVH"
     gene            1316..2104
                     /locus_tag="PKH81_05715"
     CDS             1316..2104
                     /locus_tag="PKH81_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97569.1"
                     /translation="MSDIQTSLLEIQNLSMVFGGLRAIDDASMTVRRGGITALIGPNG
                     AGKTTIFNCITGVYKPTSGTITVYNTERKAERIGGLKPNVINRKGLARTFQNIRLFSN
                     MSVLENVMIARHNSLSATLLDSIVRNRKTRLEEERVIEESHEVLKKMGLDEFANEMAR
                     NLPYGAQRRLEIARALATNPSLLLLDEPVAGMNPQETRDLEQTIHMIRDREKVSILLI
                     EHDMSLVMNVSEQIYVLDYGRMIAEGKPEEIKSNPDVIKAYLGE"
BASE COUNT          520 a          435 c          506 g          647 t
ORIGIN      
        1 cgggtattct cggcaagaat caaaagcaga aggtgtaatg tatgaagtct caaaacattt
       61 tacacgaaat tcttgattca cttaaaacca tggtttggtt tgtgattctt ctttttcctc
      121 tggttgtaat gaaagtctct gtatcctccg gggttgcctc agtcagtttc aggtgggctt
      181 atattccgtt aaccgcattg ggctcttttg tcctttcgtt tatatggaat cgtgcgcttc
      241 aatggagcga aaaccgtgct tcgatgggga atgcgaatct tcaaaaagga ttggtgtatc
      301 gcacaagcga gatattcagg caaaaaaaga cacggacatc cgcattgatt attcttgcca
      361 tcctggtact cgcgtatccc catgcgttgg gtatgtatca taccaacatc atgattaccg
      421 ctttgattta tgtaattctt gcgcttggac ttaatatcgt cgttggcctt ggaggcttgc
      481 ttaatctggg ttatgcagcc ttctttgctg ttggggcgta tacctatggt ttgttgtgga
      541 agtacgctgg ccctgtgttt accgaagctg gtattcacac cgggtggctt ttctggatag
      601 cccttcccct tgcaggcctt attgcggctc tttttggcat cttgttgagc ttgcccgttc
      661 tcaggttgcg tggtgattat cttgctatca ttaccctggc ttttggcgaa ataatcagga
      721 tgtttttaca aaactccgga gacttgaccg ggggtgcagc tggaattagt ttgattccac
      781 gtccatggtt ttttgccatg aagttgcgcc ctcctgcagc agctctgtat atttactata
      841 tcgtagtggc gcttgtgtta ctcactattt tggttgttag aagaatcgag gactctcgtg
      901 ttggtcgagc ccttgaggca atgaaagagg atgagatcgc ttgcgaggca atgggtattg
      961 atttagtcaa gaataaattg attaccttct ctcttggcgc tttttgggca ggtatagcgg
     1021 gtgtggttct tgcagctcag acaaccttta tcaacccgga cagttttacc ctgtgggaat
     1081 caataatgat acttatggct gttgttatcg gtggaactgg ctcaattccg ggcactatag
     1141 ccggagcgtt aattcttaag cttcttcccg agtatttccg cgcactctct cagtatcgta
     1201 tgctcattta tggtgtagcc atgattgtaa ttataatctt taagccagag ggtctaattc
     1261 cacgcaatcg caagaaatat actatctcga ccgttgacga aaaggaggtt cactgatgag
     1321 cgatatacaa acctccttgt tggaaattca aaacctttct atggtttttg gtggcttacg
     1381 cgcaatcgat gatgccagta tgaccgtacg ccgtggcggg ataaccgctt tgattggccc
     1441 aaacggcgcc ggcaaaacaa ccatttttaa ttgtataacc ggtgtgtata agccgacctc
     1501 cggcacaatt actgtctata acacagagag aaaagcagaa cgtattggcg gtcttaaacc
     1561 aaatgttatc aatcgtaagg gtcttgcccg aacctttcag aatatcaggc ttttttctaa
     1621 catgagcgtt ttggaaaacg ttatgattgc ccgtcataat tctctgtctg caacattatt
     1681 agattctatt gtacgcaatc gtaaaactcg acttgaagag gagcgggtaa tagaggagag
     1741 tcacgaggtc ctaaagaaga tgggcttgga tgaatttgcc aacgaaatgg cccgaaatct
     1801 tccgtatggt gcccaacgaa ggttggagat agcgcgcgct cttgctacca atccatcact
     1861 ccttctttta gacgagccgg tcgccgggat gaatcctcag gagacgcgag atcttgagca
     1921 aacgattcat atgatacgag atcgcgagaa ggtttctata ttacttatcg aacatgatat
     1981 gagtttggtt atgaatgttt ccgagcagat ctatgtactt gattatggtc gaatgattgc
     2041 agagggtaaa ccggaagaaa ttaaaagcaa tcccgatgtt atcaaggctt acctgggaga
     2101 ataatatg
//
LOCUS       DAOQXQ010000309         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_60720_length_2106_cov_2.715748, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000309 DAOQXQ010000000
VERSION     DAOQXQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60720_length_2106_cov_2.715748"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..135)
                     /locus_tag="PKH81_05720"
     tRNA            complement(64..135)
                     /locus_tag="PKH81_05720"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Glu,seq:ttc)
     gene            complement(207..1463)
                     /locus_tag="PKH81_05725"
     CDS             complement(207..1463)
                     /locus_tag="PKH81_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97570.1"
                     /translation="MSAKAHSAPWLPNRDDLERRYAHYLPDLTVLMERLEQRLRECVV
                     VSSVPTLKSRVKSFNSYYRKLLRTLSGANPPKDDLPVISDILGIRIICAFLQDLVEVE
                     QNLHRCFTVLEVERKGADRTFREFGYESTHILLAIPQELTEGLALPKALIFEIQVRTI
                     LQDAWAEVEHELVYKSEFSPFDLPLKRKLASINASLSLADIIFQEIRDYQNKLNRELD
                     KRRGSFYQQADILSDLHLAQEASLSSRLSSPDIDTNALPYVPGTIDDMILDAIEAHNA
                     GNLERASMIYTNIIEANPNSTVLSVIYKHRGMAYFAQSLYEQALADFTRSAENNPEGF
                     RSYYYIAIVLSVMGREEESLENFTKSLHLNRYQPYVYFRRAQSYYHLGLYTEALTDLD
                     KAVSLGYQKEDEAKLRRMIAAKIDMT"
     gene            complement(1575..>2106)
                     /locus_tag="PKH81_05730"
     CDS             complement(1575..>2106)
                     /locus_tag="PKH81_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNQ97571.1"
                     /translation="KSIAVNTYGMNFPHGLFVLMKDENNVERRYFMGYLLFDGWREMV
                     WQNPEYNSEVRTREVRLYPAYPTAFPYQKFTGFLITRDANHEGGDFVAYFKDVKLIYD
                     KAVLSTIRDFADEDLWGIQTKKETDRKQHEVSRFGGVQVLRFLEQEKMATEKGFTPSA
                     GSEAAAQGGAEPAAQQ"
BASE COUNT          605 a          467 c          488 g          546 t
ORIGIN      
        1 cctcgtcaaa aaaaaagggt aaggacctgt gatccttacc cgaatagtcc tgatggaccc
       61 aagcatctcc taggggaatt gaacccctgt tgtcgggatg aaaacccgat gtcctaacca
      121 ctagacgaag gagacataga ctataaagtc ttgtgcgtta cgcaactgta gtatttatag
      181 caaagagacc aatcaatgtc aatatcttag gtcatatcaa ttttagcagc tatcattctt
      241 cgcaatttcg cttcgtcttc cttttgatat ccaagagaaa cagccttatc cagatcggta
      301 agggcctcgg tatataaacc cagatgataa taactttgag cacgacggaa gtatacatac
      361 ggttgatagc gattgaggtg taatgatttg gtaaagtttt ccagagactc ttcttctcta
      421 cccatcacgc ttagaacaat cgctatgtaa taataggatc taaatccctc cggattattt
      481 tctgcgctgc gcgtaaagtc tgcaagggct tgttcgtaca gggactgggc aaaataggcc
      541 attccccgat gcttgtaaat aacagacagg acggttgaat ttgggtttgc ctcgattatg
      601 ttagtataaa tcatgcttgc ccgctccaga ttcccggcgt tatgggcctc aatggcgtcc
      661 agaatcatat cgtctatggt tcccggtaca taaggaaggg catttgtatc aatgtcgggc
      721 gatgagagtc gacttgaaag ggatgcttct tgggcaaggt gtaaatcaga gaggatatcg
      781 gcttgttgat aaaagctacc ccgccgttta tcaagttcgc ggtttagttt attttgataa
      841 tcacgaattt cctgaaatat tatatccgca agggaaagac ttgcgttgat tgatgcgagt
      901 ttccgcttta ggggtaagtc aaacggagaa aattccgatt tatataccag ctcatgttca
      961 acttcagccc aggcatcttg caaaatagtt ctaacctgaa tttcaaagat aagcgcctta
     1021 ggcaaggcaa ggccctcggt aagttcttgc ggaattgcca gtaaaatatg agtagactcg
     1081 tacccaaact cacggaaggt gcgatcggcg cctttacgtt caacttccaa tacagtaaaa
     1141 caccgatgaa gattttgctc cacttccact aaatcttgca aaaacgcaca gataatgcga
     1201 atgccgagta tatcagaaat aaccggaaga tcatctttgg gaggattagc tccactgagg
     1261 gtcctcagta gcttacgata gtagctatta aaggatttga cccgactttt aagggttggt
     1321 acagaggaca ccacaacgca ctctctcagt cgttgttcaa gacgttccat aagaacagta
     1381 agatcaggta gatagtgtgc ataacggcgt tccagatcat cacgattggg aagccaggga
     1441 gccgaatgag ccttggcaga catatatata attatacgca ttaaccaagg gcaaggcaac
     1501 ggttaaaacc aaagtaatca gagaacaaaa aaaaagagga cccgaaggtc ctccttattt
     1561 cagcttggca cagattactg ctgtgcagcg ggttcagcac caccctgagc ggcggcttct
     1621 gatcctgcag aaggggtaaa gcccttctcg gtagccatct tttcctgctc gaggaagcga
     1681 agtacctgta caccaccaaa gcgggaaact tcatgctgct tgcgatcggt ttccttcttg
     1741 gtctggattc cccagagatc ttcatcagca aagtcgcgaa tagtgcttaa cacggccttg
     1801 tcgtaaataa gcttaacatc cttgaagtag gcaacaaaat caccaccttc atggtttgca
     1861 tcgcgggtga taaggaagcc ggtgaacttc tgatagggga acgcagtggg gtatgcagga
     1921 tacaggcgta cttcacgagt tctaacctcg gaattgtact cgggattctg ccaaaccatt
     1981 tcgcgccatc cgtcaaagag gaggtaaccc atgaaatagc ggcgctctac gttgttttca
     2041 tccttcatga gcacaaacaa accgtggggg aagttcatgc cataggtatt aacagcgatt
     2101 gatttt
//
LOCUS       DAOQXQ010000310         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_60804_length_2104_cov_2.705710, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000310 DAOQXQ010000000
VERSION     DAOQXQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60804_length_2104_cov_2.705710"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKH81_05735"
     CDS             <1..289
                     /locus_tag="PKH81_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ97572.1"
                     /translation="YGVQFWKYNGFIHAKMLVIDGKTATIGSTNIDSRSFSLHFELNA
                     FFYSPEFGKECTNLFKDDLSRSKHIDPETLKKTPLRTKALWTFFRLFAPLM"
     gene            382..>2104
                     /locus_tag="PKH81_05740"
     CDS             382..>2104
                     /locus_tag="PKH81_05740"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNQ97573.1"
                     /translation="MKLSALQEARYQYAPKLPAILTAKIDTIALEEGKPTQSLANQKE
                     LKNLFKNTYGKPLVTFGKGKNPAVKKAIRVGVILSGGQAPGGHNVIAGLYDGIKKGNP
                     ESKLYGFLGGPSGLIDGKTVEIKDKFMDEYRNTGGFDIIGSGRTKIETPQQIASSMAT
                     AKKMKLDAIVIIGGDDSNTNAALLAEHFVEKGMKTQVIGVPKTIDGDLKNDMIETSFG
                     FDTACKTYSELIGNIERDANSAKKYWHFIKLMGRSASHIALECALQTQANICLISEEV
                     EAKKMTLRQITDQLCSVIAKRAAGGENFGVILIPEGLVEFIPEMKILIGELNDLMSVR
                     SDEFNRLEGFAAQATWLNKNLSKASAEAFMTLPEAIARQFLMDRDPHGNVQVSRIETE
                     KLLISMVETKLDAMKKAGTYKGSFSSYNHFFGYEGRCAFPSNFDADYCYALGFTAFIL
                     IAGGLTGYLSSVRNLTAPAKDWIAGGVPLTMMMNMEQRHGSQKPVIRKALVELNGKPF
                     KTLAKNRDVWAVKTSYTYPGAIQYYGPDSVCNQPTITLALEKGAAPAKAKAAPTKAKA
                     APTKAKAA"
BASE COUNT          597 a          576 c          463 g          468 t
ORIGIN      
        1 gtatggtgtg caattttgga aatacaacgg atttattcac gccaagatgc tcgtaattga
       61 cggtaaaaca gcgacgatcg gttcaaccaa tatcgattca agaagctttt ctctccattt
      121 tgagctcaat gccttctttt actcccctga gttcggaaag gaatgtacca acctgtttaa
      181 ggatgacctt tcacgatcca agcacataga tccagagacg cttaaaaaaa caccgctgcg
      241 taccaaagcc ctgtggacct tcttccgcct ttttgcgccc cttatgtagc aaggcttccc
      301 accttgaaac ggtacctcgt tttggatatc atgctctcac ttgcgaatgt tgcacctaaa
      361 tccatcaaac aggagatatc aatgaaactt tcagcccttc aggaagcacg ctaccaatac
      421 gcgcccaagc ttccggcaat tcttaccgca aagatcgaca ccatcgccct tgaagaggga
      481 aagccaacac aatcgttggc aaatcaaaaa gaactaaaaa acctttttaa aaacacctat
      541 ggcaaacctc tggtcacctt tggcaagggg aaaaatcccg cagtaaagaa agccattcgc
      601 gtgggtgtta tcctttccgg cggacaggcg cccggtggac acaacgttat tgccggttta
      661 tatgatggaa tcaaaaaagg aaaccccgaa tccaagctct atggatttct tggtggccca
      721 tctggcttga ttgatggcaa aaccgtagaa atcaaagaca agttcatgga tgaataccgc
      781 aataccggcg gatttgacat catcggatct ggaagaacca agattgaaac tccccagcaa
      841 atcgcttcct ctatggcaac cgcaaaaaaa atgaagctag acgccatcgt tattatcggt
      901 ggagacgatt ccaataccaa tgcggccctt cttgccgaac actttgtaga aaagggaatg
      961 aaaacccagg taatcggcgt tcccaagaca attgacgggg acctgaaaaa cgacatgatc
     1021 gagacctcct tcggattcga tacagcctgc aaaacctact ccgagctcat cggaaatatc
     1081 gaacgcgacg caaacagcgc caagaaatac tggcacttta tcaagttgat gggccgctct
     1141 gcgagccaca ttgctcttga atgcgccctt cagacccagg caaacatctg tttgatctcc
     1201 gaagaagttg aagcgaagaa aatgaccctt cgccagatta ccgatcaact gtgctctgta
     1261 atcgccaagc gtgccgcagg gggcgagaac tttggtgtta tcctgattcc cgaaggcctt
     1321 gtcgagttca tcccggaaat gaaaatcctt atcggagagc ttaacgacct gatgtccgtt
     1381 cgctcagatg aatttaaccg ccttgaaggt tttgccgccc aagcgacctg gttaaacaag
     1441 aacctgtcca aggcctctgc cgaagccttt atgactcttc ccgaagctat tgcccgtcag
     1501 ttcctgatgg acagggatcc tcatggaaac gttcaagtat cccgcattga aaccgaaaag
     1561 ctccttattt ccatggtaga aaccaaactt gatgccatga aaaaagcggg aacctacaag
     1621 ggttcattca gctcctacaa ccacttcttt ggatatgaag gccgttgcgc gttcccgtcc
     1681 aacttcgatg ccgactactg ctatgccctc ggctttaccg cctttatcct gattgccggc
     1741 ggactaaccg gatatctttc ctcggtaaga aaccttaccg cacccgcaaa ggactggatc
     1801 gccggcggcg ttcccctaac catgatgatg aacatggaac agcgccatgg ttcacaaaaa
     1861 ccggttatcc gcaaagccct tgtcgagctg aacggaaagc cctttaagac cctggcaaaa
     1921 aaccgggatg tatgggcggt aaaaacaagc tacacctatc ccggagccat ccaatactac
     1981 ggaccggatt cagtgtgcaa ccagccgacc attaccctgg ccctggaaaa aggcgccgct
     2041 cccgccaaag cgaaggctgc ccctaccaag gcgaaggctg cccctaccaa ggcgaaggct
     2101 gccc
//
LOCUS       DAOQXQ010000311         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_61049_length_2098_cov_3.062163, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000311 DAOQXQ010000000
VERSION     DAOQXQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61049_length_2098_cov_3.062163"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="thrC"
                     /locus_tag="PKH81_05745"
     CDS             complement(<1..925)
                     /gene="thrC"
                     /locus_tag="PKH81_05745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ97574.1"
                     /translation="MMYRDTRDASRTVSFRQAVLQGMDSETGGLYIPERIPTLPSSFL
                     NKIPVPSFRDIAFEVSKLFIEEEIPESDLMAIVADAFPFTAPITPVDPTTYVLELFHG
                     PTCAFKDFGARFMARTMAYFNRSEKEPLHILVATSGDTGSAVGSAFYGVEGIDVTILY
                     PKGKISPLQEKQLSTFDGNVRSLCVEGTFDDCQKLVKTAFTDATLRDTLRLSSANSIN
                     ISRLLPQSFYYIYGSLLIRDRCPYDSKLDCPSLIVTVPSGNFGNLTSGLLARAMGAPI
                     RGFIAATNANTTVPDWLASGEYTPRSSIATLS"
     gene            998..1684
                     /gene="deoC"
                     /locus_tag="PKH81_05750"
     CDS             998..1684
                     /gene="deoC"
                     /locus_tag="PKH81_05750"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ97575.1"
                     /translation="MEPINRYLDAAILKPEMTESEVENAIRDSIAWKTKTVCVRPCDI
                     PLAKKLCAGTDTQVSCVLAFPHGVAPTEVKAFEARTYIAMGTDEIDMVANYAMIRSGN
                     WSYVSKDIAAVAHLCTQSNIPLKVIFETCYLSEEEIRKTCDICIECGATFVKTSTGFA
                     QGGATKEAVAIMLDQAAGRIEVKPSGGIRSSETACEYIEMGATRLGTNWAATKDLVNG
                     TQESTSTSGY"
     gene            complement(1681..>2098)
                     /locus_tag="PKH81_05755"
     CDS             complement(1681..>2098)
                     /locus_tag="PKH81_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNQ97576.1"
                     /translation="TYMDFVPSSFQHLAGMAWRYDERNFYLLALSLDEQQGRVLNVLT
                     MIDGVFARSNETVVPLEGPLWLGLTCRNRLGRFRFSLDGKTWTELRPALDAAVLSDEH
                     GGLGFTGAFVGMFCVDTINYSTYADFKSFTYQELMD"
BASE COUNT          653 a          473 c          511 g          461 t
ORIGIN      
        1 tagaaagcgt agcgatcgag gagcgtggcg tatattcgcc agaggcaagc cagtccggca
       61 cggttgtgtt cgcgttagtc gccgcgataa agccgcgaat tggcgcgccc atcgcgcgag
      121 cgagtaaacc cgaggttaaa tttccaaaat taccggaagg aacggtaaca ataagagagg
      181 gacagtcgag tttactgtcg taaggacagc gatcgcgaat gagtaaagat ccatagatat
      241 aataaaaaga ttgtggtaac aatcgggaga tattgatgga attagcggaa gaaaggcgaa
      301 gtgtatccct caacgtagcg tcggtaaatg cggtctttac cagcttttga cagtcgtcaa
      361 aggtgccttc aacacaaagg gatcgtacgt ttccgtcaaa agtagacagc tgtttttctt
      421 gtaaggggga gatttttccc tttggataca aaatcgttac atcaattcct tctacaccat
      481 aaaaggcact acccactgcg cttcccgtat ctccagaagt ggcaaccaaa atatgcaggg
      541 gctctttttc gctccgatta aaataagcca tggttcgagc cataaaccgg gcgccaaaat
      601 ccttaaaggc gcaggtaggt ccatgaaaga gttctaacac ataggtggta ggatctaccg
      661 gtgtaatggg agcggtaaac ggaaaggcat ccgcaacgat agccataagg tctgattcag
      721 gaatctcttc ctcgataaaa agttttgaca cctcaaaggc aatatcgcga aaagaaggaa
      781 cagggatctt attcaaaaag gatgatggca aggttggtat tcgttcagga atatacaagc
      841 cgccagtctc gctatccatc ccctgcaata cggcctggcg aaaagaaacc gtgcgggaag
      901 catctctcgt atcgcggtac atcatatcaa gactatacca cagccggaac attcgttcaa
      961 tgagccattt gcgcgtattt tgtgctatag ttacagcatg gaaccaatta accgatatct
     1021 agatgcggct atactcaagc cggaaatgac agaatccgaa gtggaaaacg cgattcgcga
     1081 cagtattgca tggaaaacaa aaacggtctg tgtgcgtccc tgcgatatac ccttggcaaa
     1141 aaagttatgc gctggcaccg acactcaggt aagctgcgta cttgcctttc ctcatggagt
     1201 ggcgcctaca gaagttaagg catttgaagc aagaacatat attgcaatgg gcaccgacga
     1261 gatagacatg gttgcgaact acgccatgat tcgctcagga aactggtcat acgttagcaa
     1321 agatattgcc gctgtagctc acctatgcac tcagtcaaac atccccctta aggtaatatt
     1381 tgaaacgtgt tatctgagcg aagaggaaat cagaaagaca tgcgacatat gtatcgaatg
     1441 cggagcaacg ttcgtaaaaa cctccacagg ttttgcccaa ggaggggcga ccaaagaagc
     1501 cgtagctatt atgcttgatc aagcagccgg aagaatagaa gtaaagcctt caggtggaat
     1561 acgaagcagt gaaacagcct gtgagtacat agaaatggga gccacacgct taggcacaaa
     1621 ctgggcagca accaaagacc ttgttaatgg gacacaggaa agtaccagta cttctggata
     1681 ttagtccata agctcctgat aagtaaaaga cttaaaatcg gcataggtac tgtagttaat
     1741 ggtatccacg caaaacatcc cgacaaaggc gccggtaaaa ccaagcccgc cgtgttcatc
     1801 agataagacg gcggcatcca acgcggggcg gagctctgtc caggttttgc catccaggga
     1861 aaatctgaat ctacccaggc gatttctaca agttaagcca agccaaagag gaccttccaa
     1921 tggaacaacg gtttcgttac ttctggcaaa cacaccatcg atcattgtaa gtacattcaa
     1981 gaccctgcct tgctgttcat caagggataa ggccaaaaga tagaaattgc gctcatcata
     2041 gcgccaagcc attccagcca gatgctgaaa actggaaggg acaaagtcca tatatgtt
//
LOCUS       DAOQXQ010000312         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_61564_length_2087_cov_3.044291, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000312 DAOQXQ010000000
VERSION     DAOQXQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61564_length_2087_cov_3.044291"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKH81_05760"
     CDS             <1..529
                     /locus_tag="PKH81_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97577.1"
                     /translation="YPPLFRCYLDKDTRIPLEVFATDELKTIVEAHGLEGFMFTELWD
                     SEATASRSLSWTITKRKDNSFLVTTLIQVNGIGPYPEKKCVLDTVQIRWLCTEVRKFK
                     KESLDPDDQNMISFETSSCRFVVSVIFDKETAMVILNPMWKDTKKNYMDNVQLPLECI
                     RPFIDDLERQLTECE"
     gene            595..>2087
                     /locus_tag="PKH81_05765"
     CDS             595..>2087
                     /locus_tag="PKH81_05765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ97578.1"
                     /translation="METRIQSIAHLVVALYAMFLFLEGFRRRREIPRFNYVSLVNAGI
                     SAWNFLLFLYYGLATADAVYFVSQWGFVSVNVTIFGLFFFSWRTIFKQQHGRLLLLLA
                     LIPCITSFLSLTSPLHEVFLAGHTGFTYIPMRELSLTHGPWFIVHSVYSYVLIACTLA
                     LLIIQYVTSKRKNGIAMFLFVLSVLIFSVFLFLSNFTHLKGAIQQYAFLGHLFCVSVF
                     YWATFLDVDESVIYYGKYRFYNTVGMPVLMFNTAGNLLHLNEEARYYLEKYNCDTNSF
                     VEYIHFFDGSLFSPIDISSPPERDQSFFARNVKSTRVIYFRKADIVSGRGKMIGFSLT
                     LYDLSSMDSFVKGLESRAYTDALCQCLNRSCFEERRGRILEAATRPLAIFIADIDNLK
                     SVNDQWGHVAGDDYIRTCADIMKKFVCSTDNLYRIGGDEFIFFIPNLDVTGAERIRSG
                     IEADMAGLDKVYPCGLSIGYSVLEELDMDFEKHFTIADNEMYRHKKV"
BASE COUNT          539 a          414 c          446 g          688 t
ORIGIN      
        1 ctacccgccc ttgtttcgct gctatttgga taaggacact cgaattcctt tagaagtgtt
       61 tgccactgat gaactaaaga ctattgtaga ggctcatggg cttgaaggat ttatgtttac
      121 agaactgtgg gatagcgaag caacggcgag ccgctcccta tcctggacga ttaccaaaag
      181 aaaagataat agtttcttgg taactacact cattcaggtt aatggtattg gcccctatcc
      241 cgaaaagaag tgcgtccttg atacagttca gatacgatgg ctctgtactg aagtgcggaa
      301 attcaagaag gaatctcttg atcctgatga tcaaaacatg atttctttcg aaacaagctc
      361 ttgtcggttt gttgtctcag taatctttga caaagagaca gcaatggtta ttcttaatcc
      421 gatgtggaag gatacaaaaa agaactatat ggataacgta cagcttcccc tggaatgtat
      481 taggcccttt atagatgatc tggaaaggca acttactgag tgtgaataaa cccttcttta
      541 ctataaacaa ccgcttggct atgctatagt catccctgcg gaggcttttg taatatggaa
      601 acgcgtattc aatctatagc tcatctagta gttgctctgt acgctatgtt cctttttctg
      661 gaaggcttta gaagacgacg ggagatacct cgctttaatt atgtctctct tgtaaatgcg
      721 ggcattagcg catggaattt tctgctcttt ctctattatg gacttgccac tgcggatgcc
      781 gtttactttg tttcgcaatg gggctttgta agcgttaacg taacaatatt tggccttttc
      841 tttttttcct ggcggacgat attcaaacag cagcatgggc gcctcttgct cttgttagcc
      901 ttaatcccct gcataacaag ttttttgtct cttacctcac ccttgcacga agtgtttctt
      961 gccggtcata ctggctttac ctatattccc atgcgcgagt tatcacttac tcatggcccc
     1021 tggtttattg tacattcagt gtatagctat gtgttaattg cctgtaccct tgccctcctc
     1081 attattcaat atgtaacttc taaacgaaag aacggaatag cgatgttttt atttgtgcta
     1141 tccgtactta ttttttctgt gtttttattt ctatcaaact ttactcatct taaaggcgcg
     1201 attcagcaat atgcctttct gggacatctt ttttgcgtga gtgtttttta ctgggcaacc
     1261 tttcttgatg tagatgagtc ggttatctat tacggtaaat atcgatttta caatacggtt
     1321 ggcatgccgg tcctgatgtt taatactgcg ggtaatctgc tgcatttaaa tgaagaagct
     1381 cgctactatc tggaaaaata taactgtgac accaacagct ttgtggaata tatccatttc
     1441 tttgatggtt cgcttttttc accaattgat atctcttccc ctccggaaag agatcaatcc
     1501 ttctttgcaa gaaacgttaa aagcacccga gttatttatt ttagaaaagc agatatagtt
     1561 tccggacggg gcaagatgat aggattttca ttaacgctct atgatctttc gtctatggat
     1621 tcttttgtaa aaggcctgga atcccgagcg tataccgatg ccctgtgtca gtgtcttaat
     1681 cgatcctgtt ttgaagagag gagaggacgt atacttgaag ccgcaacgcg cccgctcgct
     1741 atttttattg ccgatattga caatcttaag agtgtaaatg atcagtgggg ccatgtcgcc
     1801 ggggatgatt atattcgtac ctgtgcagat atcatgaaaa aatttgtgtg ctccactgat
     1861 aacctctatc gaattggagg ggatgagttt atttttttta ttcctaattt ggatgttact
     1921 ggggcggagc gtattcgaag cggaatagaa gcagatatgg cggggcttga taaggtgtac
     1981 ccctgcggac tgtcaattgg ctattccgtg ttggaagaat tggatatgga ttttgaaaaa
     2041 cactttacta ttgcagacaa cgaaatgtat cgacacaaaa aagttaa
//
LOCUS       DAOQXQ010000313         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_61576_length_2087_cov_2.344488, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000313 DAOQXQ010000000
VERSION     DAOQXQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61576_length_2087_cov_2.344488"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKH81_05770"
     CDS             complement(<1..917)
                     /locus_tag="PKH81_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ97579.1"
                     /translation="MSEQLSPFKIKYGRRVFNSYSAVNSFSFALVTGNTITLYALALG
                     ASGTVVGLLSACMYLSFFAIPFGSRGVRKRGLVKTFARNWMLRNWSLIPLLAMPFLYA
                     HGHSDWAIILLLFSVLFFNFFRGVGLIANNPVIGLLAPGKDRGEYIVRLSLINNGTAL
                     LATMLLALLLWHSSGIETYNLVILIGIITGIVASALLFKLPEGRHVPDVSPVGQTDAS
                     SLSFFGSLRQASRDRNFVRFIASYLVIGLGIGMARPFIVVYCKEVYGQSDGIVTFFTI
                     CSTLGALLMGAVMRLAIDRLGARPMYIIYT"
     gene            942..>2087
                     /locus_tag="PKH81_05775"
     CDS             942..>2087
                     /locus_tag="PKH81_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNQ97580.1"
                     /translation="MKQTTTPTRSEVSPKDMWDLTTLYQNDEAWKTDLEQFPALTEGV
                     NALRDSVATSAAALADCLEKHAKALQLMEKTGNYAFLKKAEDEGNALALDRTDRYMMA
                     ATAYEAGTSWLIPAIQAIDTPTLTSWIHEEPRLEPLAIWIEKLLYLKPYILSEKEERI
                     LALQTEAAQTPRNAFSALTNVDLDFGKVRTQEGEVSLTQSSFSSLLENPDRSIRESAY
                     RQFYQGFCAHQHTIASLYTGSVHQDVAEARIRGYPSARTMALFPDRVSETVYDNLIET
                     VREYLPAVHRYYNIRKRALALSDLRHWDVYIPLVPAVQKKTSYESAVQLIGEALAPLG
                     GEYTSTLTSGLLGGWVDRYENKGKRSGAFSSGGYTGYPYILMNYKEEV"
BASE COUNT          604 a          553 c          506 g          424 t
ORIGIN      
        1 gtatagataa tgtacatcgg tcgagctcct aggcggtcta tggcaagacg cataaccgct
       61 cccatgagga gcgctcccag ggtagaacag atggtaaaaa aggtaacaat cccgtcgctt
      121 tggccataga cctctttgca atacaccaca ataaagggac gggccatacc aatgccaagg
      181 ccaataacaa gataacttgc gataaagcgc acaaaattgc ggtcccggct cgcttggcgc
      241 agggagccaa aaaaggacaa ggaagatgcg tccgtctgtc caacaggaga tacatccggg
      301 acatggcggc cttcgggaag cttaaagagt aaagccgatg cgactatacc ggtaatgatg
      361 ccaataagta tgaccaggtt gtaggtttcg atgccggagc tatgccacaa cagcaaggcc
      421 aataacatcg tggcaagcaa ggcagtccca ttattgataa gcgagagcct gacaatgtat
      481 tcacctcgat cctttcccgg agccaagagc ccaataaccg gattgttggc aataagacca
      541 acaccccgaa aaaaattaaa gaaaagaaca gagaataaga gcaggataat agcccaatca
      601 gagtgcccat gcgcatataa aaagggcatg gctaaaagcg gaatgagcga ccagtttcgc
      661 agcatccagt tgcgggcaaa ggtcttgacg agtcctcttt tgcgcactcc acggctgcca
      721 aagggtatgg caaaaaacga aagatacatg catgcactga gcaaccccac aacagttcca
      781 cttgccccaa gggcaagggc atacagggtt attgtattcc cggtgaccag ggcaaaggaa
      841 aaagagttta ctgcgctgta ggagttgaat acccttctgc catacttgat cttgaagggg
      901 gacagttgct cactcatctt cctatagtgt atcctctgac tatgaaacaa acaaccacac
      961 ccacacgaag tgaggtttcc ccaaaagaca tgtgggacct taccacccta taccaaaacg
     1021 atgaagcctg gaaaaccgat ctggagcaat tccctgcatt aacagaaggg gttaacgccc
     1081 tgcgggacag cgtagccacc tcggcggcgg cccttgccga ctgccttgaa aaacacgcga
     1141 aggcccttca gttaatggaa aaaacgggca actatgcctt tttgaaaaag gccgaggatg
     1201 aggggaacgc ccttgctctg gaccgcaccg accgatacat gatggcagca acagcatacg
     1261 aggccgggac aagctggctt atcccggcga ttcaggcaat cgacacccct acccttacct
     1321 cctggattca cgaagagcct cgccttgagc cactcgccat atggattgaa aaactgttgt
     1381 atcttaaacc gtacatcctt tcggaaaaag aagagcgaat ccttgccctg caaaccgaag
     1441 cggctcaaac tccaagaaac gccttttccg ccctgacaaa cgttgatctt gattttggaa
     1501 aggtacgcac acaagagggc gaggtctcgt taacccaaag ttccttttcc agtctgcttg
     1561 aaaatccgga tcgatccatc cgcgaaagcg cataccgaca gttttaccag ggcttttgtg
     1621 cccaccaaca taccattgcc tcgctctata ccggctccgt tcatcaagac gttgccgaag
     1681 cgagaatcag gggctacccc tctgcccgga cgatggccct tttccccgat cgcgtaagcg
     1741 aaaccgtata cgacaatctg atcgaaaccg ttcgcgagta tcttcctgca gtacaccgct
     1801 actacaacat acgcaaacga gccctggctc tttctgattt acgccattgg gacgtgtata
     1861 ttcccctggt tcctgccgtc caaaaaaaga caagctatga aagcgccgtt caactgatcg
     1921 gggaagccct tgctcccctt ggaggggaat acaccagtac ccttaccagt ggattactgg
     1981 gtggctgggt agatcgttac gaaaacaagg ggaagcgttc gggggccttc tcttcgggag
     2041 ggtataccgg atatccgtat attctgatga attataaaga agaagta
//
LOCUS       DAOQXQ010000314         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_61658_length_2085_cov_3.658128, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000314 DAOQXQ010000000
VERSION     DAOQXQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61658_length_2085_cov_3.658128"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKH81_05780"
     CDS             complement(<1..238)
                     /locus_tag="PKH81_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ97581.1"
                     /translation="MPTIKDVARLAGVSYTTVSHVINGTRPVAEDTRDRVEKAIKELG
                     YRQNMIARGLRKGESMTIGVVSHSSSDAYFSEVLH"
     gene            complement(240..1601)
                     /locus_tag="PKH81_05785"
     CDS             complement(240..1601)
                     /locus_tag="PKH81_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ97582.1"
                     /translation="MQSENINRSLIRIGLPILAGMCVSQVQQITDQAFLGHASAEYLS
                     AVGNAGFTIWTTISFLFALGTGTAILVSQKIGEGAKEKAEEILGSTFVFSSFFSVAIF
                     LVWFFGNVTIFRLMGLSEPILSHAALYTRIYTFGIVFSGITSTANAVYNGTGFTRPLM
                     ISSLLRAGINIILDWILIFGNLGFPQLGIAGAAIASLTADIIGGSYTVYAAFSRRLPV
                     RLCLSAIKKANIKTYWQVIKIGVPASLEEFAWNIGNILLIRFLNMVDPLAAGIYTIVA
                     SITLVPALFFMAIGNAAMTISGRKTGEGRPADIKKTGRHALGISWAIALVFFAFFVLI
                     PDVFVRFFTQDQAILAQTGPLMLVCAFALFPRAANLIFGGGIRGMGDTKWMFMTQFCG
                     TIFVLVLARIMIFNLAFGILGLFVAVFFDELLRGIINGFHFHGHVRAKVRALAQTNAE
                     KDM"
     gene            complement(1714..>2085)
                     /locus_tag="PKH81_05790"
     CDS             complement(1714..>2085)
                     /locus_tag="PKH81_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNQ97583.1"
                     /translation="NAEEVETLTRTNYDPLQYYYANRELKQVIDQIRRGHFSPENVDL
                     FKPLVDSLLYQGDQYLLLADYQSYIACQDRVSQAFRDTESWTRMSILNTARMGKFSTD
                     RTISEYSQDIWGIEPVHIGLS"
BASE COUNT          611 a          484 c          472 g          518 t
ORIGIN      
        1 catggagaac ttccgaaaaa taagcatcgc tactggaatg gctaacaacg ccgatggtca
       61 tgctttcgcc cttgcgtaag ccgcgagcga tcatgttttg tcggtaaccc agttctttaa
      121 tcgccttttc tacacgatcc cgggtgtcct cggctacagg ccgtgttccg tttattacat
      181 gggaaacagt agtgtacgaa actcctgcaa gtcgagcgac atccttgatc gtaggcatat
      241 tacatatcct tttcggcatt cgtctgtgct aaagccctta ccttggcacg aacatgccca
      301 tgaaaatgaa atccgtttat tataccacgc aagagctcat caaaaaagac agccacaaaa
      361 agccccagaa tcccaaaggc taaattaaaa atcatgattc gtgcaagaac aagaacgaaa
      421 atggtaccac agaattgcgt cataaacatc catttggtgt ctcccattcc gcgtattcct
      481 ccgccaaaaa ttaagttagc cgcacgggga aacagtgcaa aggcacacac aagcattaag
      541 gggccagtct gggcaagaat tgcttggtct tgggtaaaga acctgacaaa gacatcagga
      601 ataaggacaa aaaaggcaaa gaataccagg gcgatagccc agctgatgcc caacgcgtgg
      661 cgtcccgttt ttttgatatc ggccggccgc ccctctccgg tttttctgcc agatatagtc
      721 atagccgcat ttccgatcgc cataaagaaa agagcgggaa caagagtaat ggaagcgact
      781 atcgtgtaaa taccggcggc aagtggatct accatgttta aaaaacggat aagcagaatg
      841 tttccgatat tccaggcaaa ctcctcaaga cttgccggca cgccaatttt gataacttgc
      901 cagtaggttt tgatgttcgc ttttttaatt gcggaaagac agagcctgac cggaagtcgg
      961 cgagagaagg ctgcgtacac cgtataactt ccgccaataa tatccgctgt taaactggcg
     1021 atagcggcgc cggcaatgcc aagctgggga aacccgagat ttccaaagat caggatccag
     1081 tccagaataa tgtttattcc ggccctcaat agagaggata tcatcagagg ccgggtaaaa
     1141 ccggttccgt tgtatacagc attcgcagtt gaggtaatcc ccgaaaacac gataccaaag
     1201 gtgtagatac gagtgtacag ggctgcatgc gaaagtattg gctccgataa tcccattaag
     1261 cggaaaattg ttacatttcc aaaaaaccat accaaaaaaa tagcaacgga aaagaaactg
     1321 gaaaacacaa aggttgaacc cagaatttcc tctgcttttt cttttgcccc ttctccaatt
     1381 ttttggctta ccagaattgc cgttcctgtt cccagggcaa acaaaaagga aatggtggtc
     1441 caaatggtaa atccggcatt accaacggcc gaaaggtatt cagccgaggc atgacccaga
     1501 aaagcctgat cggtaatctg ttgaacttga cttacacaca taccggcaag aatgggtaag
     1561 ccgattcgta taagagatcg attgatattt tctgattgca tctttttcct ccagactgcg
     1621 tatcataggg cgcaatcgtt tgctgttagt aataaaacat tacgcaaacg tttgcaatac
     1681 cctttagagg aaaaataact ataacttgta taattatgac aagcctatgt gcaccggttc
     1741 aatgccccag atatcctgcg agtattcact tattgtgcgg tctgtagaga atttccccat
     1801 ccgggctgta ttcaggatgg acatgcgtgt ccacgattct gtatcgcgga atgcctggct
     1861 tacacggtcc tgacatgcaa tataggactg ataatcggca agcaggaggt attgatcccc
     1921 ttgatacaac agtgagtcaa ccaggggttt gaataagtcc acgttttcgg gagagaagtg
     1981 tccccttcgt atttgatcaa taacttgttt aagctcgcga ttggcgtaat agtattgcag
     2041 cggatcgtag tttgtccggg taagggtctc tacttcttct gcatt
//
LOCUS       DAOQXQ010000315         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_62264_length_2073_cov_2.039643, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000315 DAOQXQ010000000
VERSION     DAOQXQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62264_length_2073_cov_2.039643"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..364)
                     /gene="queD"
                     /locus_tag="PKH81_05795"
                     /pseudo
     CDS             complement(6..364)
                     /gene="queD"
                     /locus_tag="PKH81_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
     gene            427..1974
                     /locus_tag="PKH81_05800"
     CDS             427..1974
                     /locus_tag="PKH81_05800"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNQ97584.1"
                     /translation="MNKHLSSFISAIKSLERVGDCDPEVTDLVYDSRKAKPGALFFAM
                     SGIHTDGSAYISQAIQGGARVIIHESPLASYDNKVVYLRVESARFAMSAVSAAFYDYP
                     SRDMAIIGVTGTEGKSTTVSLIWQLLTLAGKKAGFISTVDYCLGAEVMANPEHQTTPE
                     ATTIHEKLAAMRDSGCEFAVIESSSHGLSPRTNRLGDVLFDAAVLTNLTHEHLEFHGT
                     REQYKTDKANLFRSLDSHDHLKKGRLIPSFGVVNEEDTASSYFRSATSKPVYGYSTWA
                     DCPRGACATGITEDAQGSTFTFSSSAQVFRARINLPGAFNVYNTLAAAITVFRIAEIP
                     LTDLVGLFEKLTPIKGRMTVVDEGQPFEVLVDYAHTPSSFQAIFPPLRRRISGKIISL
                     FGSGGERDTVKRPEQGRIAADYSDIVILSDEDPRGEDPVELLQAIAAGCPERTIGTDL
                     FIIPDRSEAIRKAISLAGSGDLVLLLGKGHENSIIYADRTIAYDEIQEARAALREAGF
                     GQGKAAL"
BASE COUNT          523 a          556 c          527 g          467 t
ORIGIN      
        1 tatgattatg cataataacg agcacggctc gtcggggttt cgtacacatc gactgcccat
       61 agcggtaccg cgggtacaag ggctgtgagc cgacgataga ttaactcggc gatacgctcg
      121 gcgctggggt tttgacaaaa atccggaatg tcgttcagat gtgaatgatc cagggaagcg
      181 catacctcgc gaagggcctt ttttaagacc ccaaaatcaa cgagcatgcc gccttcgtca
      241 aggctttgac ctcgggcatg ggcaagaacg cgatagtttg gccatgaagc cgttcacatt
      301 taccatgaaa atcttttaaa aaatgagcgg cggcgaactc cgcttccaca cgtacttcat
      361 acataccgtt ataatatcga caaggtgttg ccaattcaag tgtgcgttgg tacaatcaag
      421 cccagcatga ataaacacct gtcttccttt atttccgcga tcaaaagcct ggaacgcgtt
      481 ggcgattgtg atcccgaagt aacagatctt gtctacgact cgcgcaaggc caaaccgggc
      541 gcgctttttt ttgcgatgag cggtattcat accgacggct ctgcctatat ttcgcaggca
      601 atacaaggag gagcccgcgt aataatccac gaaagtccgc ttgcatcgta tgacaataag
      661 gttgtgtatc ttagggtaga aagtgcgcgg tttgccatgt ctgccgtatc ggcagccttt
      721 tacgattatc cctcgcggga tatggcgata atcggggtta ccggtaccga agggaaaagc
      781 acaaccgtgt cccttatctg gcaattgctg accctggcag gaaaaaaagc aggttttatt
      841 tccaccgttg actattgcct tggcgccgag gttatggcaa atccggagca tcaaacaacg
      901 ccagaggcta caaccattca tgaaaaactg gcggcaatgc gagacagcgg atgcgagttt
      961 gcggttatcg aatcatcctc ccacggcctt tctccccgaa caaaccggct cggcgatgtc
     1021 ctttttgacg cggcggtatt gacgaatctg acccatgaac accttgagtt tcacggaacg
     1081 agggagcaat ataaaacaga caaggcgaac cttttccgat ccctggattc ccacgatcat
     1141 cttaaaaaag gaaggctcat cccctccttt ggcgtggtaa acgaggaaga taccgcctca
     1201 tcctacttcc gctcggcaac ctcaaagccg gtgtacggct attctacctg ggctgactgc
     1261 ccccgcggtg cctgcgccac cggaattacc gaggacgcac aagggtccac ctttaccttc
     1321 tcttcctccg ctcaggtatt tcgcgcgcga atcaatcttc ccggtgcatt taatgtatac
     1381 aataccctgg cggcagccat aacggtgttc cgcatcgcgg agattcccct gacggacctg
     1441 gtagggcttt ttgaaaaact tacccccatc aagggacgaa tgaccgtcgt tgatgaagga
     1501 cagccctttg aagtccttgt cgattacgct cataccccct catcgtttca agcaatcttt
     1561 cccccgcttc gaaggcgtat ctcgggaaag ataatcagct tgtttggctc gggaggggag
     1621 cgggatacgg taaagcggcc ggaacaggga cgcatcgccg cagattactc cgacattgta
     1681 atcctttccg acgaggatcc ccgcggcgaa gaccccgtcg aattactaca ggcaattgcc
     1741 gccggatgtc ccgaacgaac aataggaacc gacttgttta tcatccccga ccgaagcgaa
     1801 gcgatacgaa aggcgatatc gcttgcagga agcggggact tggttctgtt attggggaag
     1861 ggacacgaaa actcaataat ctacgctgac cgaacgatag cctacgacga gatacaagag
     1921 gcgcgagctg ccctccgcga ggcggggttt ggccagggta aggcagctct gtgaccatac
     1981 ccttttataa aatacacatc gccggaaacg gttttatcct tatcgacctt accgcccttg
     2041 aagccgcgaa caaggggaaa ccagcgttgg aaa
//
LOCUS       DAOQXQ010000316         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_62668_length_2064_cov_2.823295, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000316 DAOQXQ010000000
VERSION     DAOQXQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62668_length_2064_cov_2.823295"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKH81_05805"
     CDS             <1..436
                     /locus_tag="PKH81_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ97585.1"
                     /translation="GVIGALLAATTRGLSNDIFFQVGLLTTIGLSAKNAILIVEFAKE
                     LFDKGMGIKQAVIEASRMRLRPILMTTLTTVIAMVPMAFFPGDGGEMMQPIGITMVGG
                     LLSGAVMTLFVTPIMYHLFNKRRERRFDDPESLQNMLATYEP"
     gene            474..971
                     /locus_tag="PKH81_05810"
     CDS             474..971
                     /locus_tag="PKH81_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNQ97586.1"
                     /translation="MSKKECPCGSGKNRADCCEPIIKGKTPAPTAEALMRARYSAYVL
                     GEIPFIADSCVRGEGDHEIDLEETKKWSKESEWHGLKIHAVTGGAASDSEGQVEFSAF
                     YSRAGLKDEHRELAFFKKIDNKWLYAEGRLVPTTIVRSGAKVGRNDPCPCGSGKKYKH
                     CCGRA"
     gene            1049..1432
                     /locus_tag="PKH81_05815"
     CDS             1049..1432
                     /locus_tag="PKH81_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97587.1"
                     /translation="MPRLKIFSVALLGTAAFLLLWWPFSHWFFYRWYNDLLGFTFSAT
                     HDGLVKMIGTCGLMPVLSLAALAMKPKNNVPLVAAVSIFSVFLSLTFLFLIARGDFPP
                     REMINVVMTFFLGVSLPLFYRWAQR"
     gene            complement(1434..>2064)
                     /locus_tag="PKH81_05820"
     CDS             complement(1434..>2064)
                     /locus_tag="PKH81_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ97588.1"
                     /translation="MHVAFPVMANELLWGLGITLQSVIFGRTDTNALAAFNITNVVSN
                     LTWVYYIGLANGASVLIGKKIGEGNQGLARDWAFRCVKISVLTAPILAGILIVLKYTI
                     PSFFNITDEALTSMGSFFILLAIAYPFRSFNISMVIGVCRAGGDTRFCIVYDLVFMWL
                     FALPGAAIAAFVCHAAPWVIYLLVVSEEALKVSLGYARLKSGKWLHDVT"
BASE COUNT          529 a          465 c          510 g          560 t
ORIGIN      
        1 tggggtcatc ggtgccttgc tggcggccac cacgcgtggt ctgtcgaatg acatattctt
       61 ccaggtgggt ttgttgacta ccatcgggtt gtcggcgaaa aacgccatcc tgattgtgga
      121 attcgccaaa gagctgttcg ataaagggat gggcatcaaa caggcggtga ttgaagcatc
      181 acgtatgcgt ctgcgcccga tattgatgac caccctaact acagtaatcg ctatggtgcc
      241 aatggccttt ttccctggag atggcggaga gatgatgcaa ccgattggta taaccatggt
      301 aggaggcctg ttatctggag ccgtaatgac cctgtttgtt acaccaataa tgtatcatct
      361 ttttaataaa cggcgagaga gaaggtttga tgatcctgaa tctttacaga acatgcttgc
      421 aacgtatgag ccttgattta ccattgtgcc tctctgtgct atagtcattt attatgagca
      481 agaaagaatg cccttgtgga tcgggtaaga atcgggccga ctgttgtgag ccgattataa
      541 aggggaaaac ccccgcccct actgccgaag ccctgatgcg ggcgcgatac agcgcatatg
      601 tattgggaga gattcccttt atcgcagatt cctgtgttcg cggagaggga gatcatgaaa
      661 ttgatcttga agaaaccaaa aagtggagca aggaatccga gtggcatggc cttaagattc
      721 acgcagttac cggcggtgct gcctccgata gcgaagggca ggtcgagttt tccgccttct
      781 attctcgcgc aggattaaag gatgaacacc gggagcttgc cttttttaag aagatagaca
      841 acaaatggct gtatgcagag ggcaggcttg ttccaactac catcgttcgc tctggcgcca
      901 aggtaggacg taacgatccc tgtccgtgtg gaagcggaaa aaaatacaag cattgttgcg
      961 gccgcgctta gatttgcaaa cataacacgc cttctttccc tgggcgtggt atacttaccg
     1021 tacgtactta gtaactggag ggtataccat gccgagactc aagatctttt ccgtagcatt
     1081 gcttggaact gccgcctttt tgcttttgtg gtggccgttt tctcattggt tcttttaccg
     1141 ttggtataat gatctattag gttttacctt ttctgcgaca catgacggac tggtcaagat
     1201 gattggtact tgcggcttaa tgccggtgtt aagccttgcc gctctggcca tgaagcctaa
     1261 gaataatgtt ccattggttg cggcagtttc tatcttttct gtttttcttt cgctaacttt
     1321 tttgtttctt attgctcgag gagactttcc tccgcgagaa atgattaatg ttgtgatgac
     1381 cttttttctt ggggtgagcc ttccgctgtt ttatcgatgg gcgcaacgct gatctatgtt
     1441 acgtcgtgaa gccattttcc gcttttaagt cttgcatagc caaggcttac ctttagcgct
     1501 tcctcgctta cgactaatag ataaataacc cagggggctg catgacagac aaaggctgcg
     1561 attgccgccc ctggtaaggc aaagagccac ataaaaacca aatcgtagac aatgcaaaaa
     1621 cgggtgtctc ccccggctcg gcacaccccg ataaccatcg agatattaaa agagcgaaag
     1681 gggtaggcaa tagcaagtaa tataaagaaa gatcccatgg aggtgagcgc ttcatcggta
     1741 atattaaaaa aggacgggat cgtatattta agaacaataa gtatgccagc aaggattgga
     1801 gcggttaaca ctgatatctt tacgcaccgg aaagcccagt cgcgggcaag cccttgattc
     1861 ccctctccta ttttttttcc gataaggaca gacgcaccat tagcaagccc aatgtaatat
     1921 acccaggtca ggttagacac aacatttgtt atattaaagg ccgccagagc attcgtgtcg
     1981 gttcttccaa aaataacgct ttgcaaggta atccccaaac cccagagtaa ttcattagcc
     2041 attacaggaa atgcaacgtg cata
//
LOCUS       DAOQXQ010000317         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_62775_length_2062_cov_2.361236, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000317 DAOQXQ010000000
VERSION     DAOQXQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62775_length_2062_cov_2.361236"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1749
                     /gene="dnaK"
                     /locus_tag="PKH81_05825"
                     /pseudo
     CDS             34..1749
                     /gene="dnaK"
                     /locus_tag="PKH81_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            1811..>2062
                     /locus_tag="PKH81_05830"
     CDS             1811..>2062
                     /locus_tag="PKH81_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNQ97589.1"
                     /translation="MATKRDYYEVLGVQKNASKDDIKKGYRKLAIQYHPDKNPGNKEA
                     EEKFKEATEAYEVLSDDQKRQVYDQYGHAGLEGMGGPGGP"
BASE COUNT          554 a          513 c          557 g          438 t
ORIGIN      
        1 gtttagcgaa aaataagagg cgcgcccgaa ccgggccggc gccgcatcga gacgaaggac
       61 atccagcccg tgaaggtcgc cgccagctcg ctccggaaag ggtccgtcgt cgatatcgac
      121 ggcaagctgt attcacccca ggagattagc gcttttatcc ttcaaaaaat gaagaaaaca
      181 gcagaagact atttgggcga aacggttacc gaagcggtaa ttaccgttcc tgcctatttt
      241 aacgacgcac aacgccaggc aaccaaggac gccggtaaga ttgccggtct tgaggttaag
      301 cgcattatca acgagccaac cgctgcctcc cttgcctttg gctttaacaa ggattccaag
      361 aaggaaaaaa ccatcgccgt ttatgacctt ggtggcggta cctttgatat ttcgattctc
      421 gagctcggtg acggtgtttt cgaagttaag tctaccaatg gtgatactca ccttggtgga
      481 gacgactttg accggcgtat tatgaattgg cttgtggatg agtttaaggc tgattcaggc
      541 attgatctgt cacaggaccg catggccctg cagcggcttc gcgaagcggc ggaaaaggca
      601 aaggttgagc tttctgcggt taccaatacc gaaatcaacc tgccctttat tactgccgac
      661 gcctcaggtc ccaagcactt gcaaaagagc ctctcccggg ccaagttcga acagatgtgc
      721 gatgacttgt ttgagcgaac caaggaaccc tgccgaaagg ctatcaagga tgctggaatc
      781 agcatcgacc agattgacga tattcttctt gtcggcggtt caacccgcat gcccaaggtg
      841 atgcaaatcg taaaggaatt attcggtaag gaaggctcca agggtgttaa ccccgacgaa
      901 gcggttgccg caggtgctgc cattcaggga ggtattcttg gtggggacgt taaagacgtt
      961 cttctccttg acgttacccc gctttccctt ggtatcgaga caatgggtgg cgtatttacc
     1021 cgtctgatta accgaaatac caccattccg actcgaaaga gccaggtctt ttcaacagct
     1081 gccgacggac aaaccgctgt atccattcac gttcttcaag gagagcgcga aatggccggt
     1141 cagaaccgga cccttggcaa atttgacctt gtcggtattc ctcccgctcc gcgtggagtg
     1201 ccacaaatcg aagtaacctt cgatattgac gcaaatggta tcgttcatgt cgcggcaaag
     1261 gatttgggaa ccgggaagga acagaaaatc cgtatcgaaa gctcctccgg tctctccgaa
     1321 agcgagatag atcgcatggt caaggaagct gaggcaaatg cagaggcaga caaggctgaa
     1381 cgggaaagag ttgaagttcg taatgaagcc gactcgatga tttacaccac cgagaagacc
     1441 ctaaaggata tggccgacaa ggtggctgct gccgacaaac aacagatcga aagcgctgtt
     1501 gcagatctta agcaagcctt ggagggcgga gaccttgagg caatcaaggc aaagacagag
     1561 gcgctcaagc aggcttcata caagattgcc gaagagatgt acaagcagca agccgcttcc
     1621 ggcgctgccc cgggaggcga ggcctctgat gctggcttct ccggctctgc cgaaggtgct
     1681 tccgccggtc ccaataaggg ttcggccgat gacgttgact atgaagtagt taacgacgat
     1741 aataaataat tcttaactga caaggggccc cggttggggt cccttgccta tcctgtatgt
     1801 ggagtcccct gtggcaacga aaagagacta ttatgaagtt ctgggcgtac aaaaaaacgc
     1861 ttccaaggac gatataaaaa aaggataccg taagctcgct attcagtatc atcccgataa
     1921 aaaccccgga aataaagaag ccgaagagaa attcaaggaa gcgaccgagg cctacgaggt
     1981 gctttccgac gatcaaaaac gtcaggtgta tgaccaatat ggacacgcag gccttgaagg
     2041 catgggtggt cctggcggtc cc
//
LOCUS       DAOQXQ010000318         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_62847_length_2061_cov_1.845962, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000318 DAOQXQ010000000
VERSION     DAOQXQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62847_length_2061_cov_1.845962"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKH81_05835"
     CDS             <1..593
                     /locus_tag="PKH81_05835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ97590.1"
                     /translation="DEVDRMLDMGFINDITDIFTNISRNRQSLFFSATMEPKIASLIE
                     KFSRDPVTVSVKEGATADSVSQDIVYYSGNEDRIAKLHDLLISGACEKTIIFDDTKRS
                     VERLGRELNARGFRVDQIHGNKSQAQRARAIKRLKMNEIDILVATDVAARGIDVSDIS
                     HVVNFSAPNTYDDYVHRIGRTGRAGKTGKALTFLPK"
     gene            637..>2061
                     /locus_tag="PKH81_05840"
     CDS             637..>2061
                     /locus_tag="PKH81_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNQ97591.1"
                     /translation="MAIIGIDLGTTNSLAAVWKDGEPVIIPNAHGERLTPSVVSIDDA
                     HTVLTGASAKERLITFPDHTIASFKRNMGLSSYYQIHRKMYSAVDLSSLILSQLKKDA
                     ERFLGEPVTEAVISVPAYFNQNQRKATKRAGEMAGLVVERLVSEPTAAALFYGIDAQP
                     DMKTALVFDLGGGTFDVSILEFFDGVVDVKAVSGDNHLGGEDFTRLISAWFILEHSLE
                     NKLSSHELSCLHKASEIAKQQITSNDVSGPQASGVQLELKATGKTYTSTLTVSIFDAL
                     CTDLLSRLQAPVVRALKDAGLHPQDIDKVILMGGATRMRHVCDLVHSLFPNRVITSYN
                     PDETVALGAAVLAAMKAHHHQLKETVLTDICPFSLGIEVHQGDDSSRVKKSFFDPVIE
                     RNTPIPVSIVNQYLTSVPGQKQVRINVYQGESLKVSENLKLGEFCVDVPINPDGLEVI
                     DVRFTYDINGLLEIEATIVSTGDRT"
BASE COUNT          523 a          489 c          495 g          554 t
ORIGIN      
        1 ttgatgaggt agatcgcatg cttgatatgg gttttataaa cgacatcacc gatattttta
       61 ccaatatctc ccgtaatcga caatccctct tttttagcgc gactatggaa cccaagattg
      121 cttcccttat cgagaagttc tccagggatc cggtaactgt atccgttaaa gaaggtgcta
      181 ccgccgacag cgtgagtcag gatattgtct attactccgg taatgaagac cgcattgcca
      241 agttgcacga tcttcttatc agcggagcct gtgaaaaaac gatcattttt gacgacacga
      301 aacgttcggt tgaacgactt ggtcgagagc ttaatgcgcg gggctttcgc gtagaccaaa
      361 tacacggtaa taaaagtcag gctcaacgtg ctcgagcgat caagcggctc aagatgaacg
      421 aaattgatat tcttgtggcg accgatgttg ctgcccgagg cattgatgtt tccgatattt
      481 ctcatgtagt caatttttcc gctcctaata cctacgacga ttatgttcat agaattggcc
      541 gtaccggtcg tgcgggaaaa acgggaaagg cgcttacctt cctgccaaaa taatcatatt
      601 tgggtgtata ctgtacaata aacacaggaa cccaaaatgg cgattattgg cattgattta
      661 ggcactacaa acagtttagc ggcagtgtgg aaagacgggg agccggttat cattcctaac
      721 gcgcatggag agcggttaac cccgtctgtt gtatcaatcg atgatgcaca cacggtgcta
      781 accggcgcaa gcgcgaagga acggctcata accttccccg atcacaccat tgcctccttt
      841 aagcgaaaca tggggctttc ctcctattat caaatacatc gcaagatgta ttccgcagtt
      901 gatctttcat cactgattct ttcgcaatta aaaaaagacg cagagcgatt cttgggtgag
      961 ccggtaaccg aagcggttat cagcgtgccg gcctacttta atcaaaatca gcgaaaggca
     1021 acaaaacgtg ccggagagat ggcgggtctt gtggtggagc gcctggtaag cgagccaact
     1081 gcagcggccc ttttctacgg gattgacgcg caacccgata tgaaaaccgc cctggttttt
     1141 gatcttggtg gtggaacctt tgatgtgtct atccttgagt tctttgatgg tgtggtggac
     1201 gtcaaggcgg tctcgggaga caaccatctg ggaggagaag attttacccg cctcatctcg
     1261 gcctggttta tccttgaaca ttccctggaa aacaaacttt cttcgcatga gctttcctgt
     1321 cttcataagg cttccgagat tgcaaagcag cagattacca gtaatgatgt ctctggtcct
     1381 caagcttcgg gcgttcagct tgaacttaaa gctaccggta agacctatac ctccacttta
     1441 actgtatcca tttttgatgc cctctgtacc gatcttctct ctcgtcttca ggcaccggtt
     1501 gtccgggccc tcaaggatgc aggattacat ccgcaagaca ttgataaggt catcttgatg
     1561 ggtggtgcca cacggatgag gcatgtctgc gatttggtgc attccttgtt cccgaatcgc
     1621 gttattacca gttataatcc tgacgaaaca gttgccttgg gtgcggctgt gctagccgca
     1681 atgaaggctc atcatcacca acttaaagaa accgtcctta ccgatatctg tccattctcc
     1741 cttggtatcg aagttcatca aggtgatgat tcttcccgcg tcaagaaatc tttttttgat
     1801 ccggtaatag agcggaacac tccaatcccg gtaagcatcg tcaatcaata tctaacaagt
     1861 gttcccgggc aaaaacaggt tcgtatcaat gtgtatcagg gcgagtcact taaggtgtcg
     1921 gaaaacctga agctgggcga gttttgcgtt gatgttccaa tcaatccaga tggccttgag
     1981 gtaatcgatg tgcgctttac ctacgatata aacgggcttt tggaaattga ggcgaccata
     2041 gtctctacgg gagaccggac c
//
LOCUS       DAOQXQ010000319         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_62937_length_2059_cov_2.608283, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000319 DAOQXQ010000000
VERSION     DAOQXQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62937_length_2059_cov_2.608283"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKH81_05845"
     CDS             <1..559
                     /locus_tag="PKH81_05845"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNQ97592.1"
                     /translation="EAARRIRASGKECILHQPMQAINLDMDPGPGAIKPGMGADTIRD
                     IVRKNLADIGPVAGMNNHEGSLISADRDAMAAVLDVALEAGIYYLDSRTNAKTAVPAI
                     ARDRKMTIWERAVFLDNSQDRMMIMDAISSGMKIAERKGSAIMIGHIWSNDLADILTE
                     MYPELVSQGFSLSTIARLATEAEDF"
     gene            559..1578
                     /gene="tsaD"
                     /locus_tag="PKH81_05850"
     CDS             559..1578
                     /gene="tsaD"
                     /locus_tag="PKH81_05850"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNQ97593.1"
                     /translation="MKILGIESSCDETAAAVVENGKHIISSVVATQIPFHTMYNGVVP
                     EIASRKHTEWILPVVKQALSEAELTIRDIDGIAVTNSPGLMGSLLVGLTFAKTLAWSI
                     DAPFIAVNHMLAHLYAAHINEEIPYPYLGLLVSGGHSIICRVSGFDQIEILGTTIDDA
                     VGEAFDKVAKFYNFGYPGGVAIDKLAREGNPAAASFPMPSLHKGGHEYDVSYSGLKTA
                     VINQIDQFWNPAFEKTPQHIAAAFQETAVRILLSRLLKAVRDTGLTTIVAGGGVAANS
                     RLRELLASEQNLRCIFPPLKLCGDNGAMIAGIGYHYLVRGDRSPWSIGASARVQGFRK
                     SGRPS"
     gene            1618..>2059
                     /locus_tag="PKH81_05855"
     CDS             1618..>2059
                     /locus_tag="PKH81_05855"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNQ97594.1"
                     /translation="MSKSFSLDSVIRKIPDFPKPGILFYDITGILVNPPAFKYCIDQM
                     VKMYKGERIDAIAAIESRGFVFAAPFAERMGIPLILIRKKGKLPGQTWSCSYALEYGT
                     AELEVHKADVQGGQRILVMDDLIATGGTLNAARNVLEQGGARVAG"
BASE COUNT          493 a          485 c          558 g          523 t
ORIGIN      
        1 agaagcagcg cgaagaatcc gcgcttcggg gaaggaatgc atcctgcatc agcccatgca
       61 ggcgatcaat cttgacatgg atccggggcc tggcgcgatt aagccgggga tgggcgcgga
      121 tacaatcagg gatatagtac gcaagaatct cgcagatatt ggacccgtgg cagggatgaa
      181 caaccacgag ggctctctta tatccgcaga cagagatgca atggctgctg tccttgatgt
      241 agcccttgaa gcaggtattt attacctgga ttcgcggact aacgcgaaaa ccgctgttcc
      301 ggcaattgct cgggaccgga agatgacgat ttgggagcga gcagtgtttt tggacaacag
      361 tcaggaccgc atgatgatta tggacgccat ttcctctggc atgaagattg cggaacgcaa
      421 gggctctgcg atcatgatcg gtcatatctg gtctaatgac ctggctgata ttttaaccga
      481 gatgtatccg gaactggtaa gccagggatt ctctctttct accatagcgc ggttagcaac
      541 cgaggcagag gatttttaat gaaaattctt gggatagaaa gctcttgtga tgaaaccgct
      601 gcggctgtgg tagaaaacgg aaaacacatt atttcaagcg ttgttgcaac gcaaatacct
      661 tttcatacca tgtataacgg tgtcgtaccc gagatcgcca gccgtaagca taccgaatgg
      721 atcttaccgg tggtaaaaca ggccttaagc gaggctgaac tgactatcag ggatattgac
      781 ggtatcgccg taacaaactc tccgggtttg atgggttcgc tgctggtagg ccttaccttt
      841 gccaaaaccc tggcttggtc gattgatgct ccctttatcg ctgtaaatca tatgcttgcc
      901 cacttatatg ctgcgcatat taacgaagag ataccctatc cttatttggg tttactggta
      961 tcgggcggac attccattat ttgccgagtt tccggctttg atcagataga gattcttggc
     1021 accaccattg acgacgcggt gggagaggcc tttgacaagg tcgcgaagtt ttataatttt
     1081 ggctatcctg gtggggtggc catcgacaag cttgcccgtg agggaaaccc ggccgccgca
     1141 tccttcccca tgccttcttt acacaagggc ggtcatgagt atgatgtgtc gtattccggg
     1201 ttaaaaacgg cagttatcaa tcagatagat cagttttgga atcctgcctt tgaaaaaacg
     1261 ccgcagcaca ttgcagccgc gtttcaggaa acggcggttc gcatcttgct ctcgcgtctt
     1321 ttaaaggctg tacgcgacac gggtcttaca accattgtag ctggcggagg tgtggcagca
     1381 aactcccggt tgcgggaatt actggcctct gaacaaaatc ttcgctgtat atttcccccg
     1441 ctcaagctct gcggtgacaa cggcgcgatg atagccggta tcgggtatca ctatctggta
     1501 cggggagaca gaagcccttg gtcaatcggc gcaagtgccc gcgtgcaggg ctttcgtaaa
     1561 tccggacggc cttcctgaca ttgacgagct ctgggccaat atactagtat actagccatg
     1621 agcaaatcct tttcgcttga ctcggttatc agaaaaattc ccgattttcc caagccggga
     1681 attctgtttt atgacattac cgggattctg gtgaatcccc ctgcctttaa gtattgcatt
     1741 gaccaaatgg tcaagatgta caagggagag cggatagatg ccatcgcggc gattgaatca
     1801 cgaggatttg tgtttgccgc gccctttgcc gagcgcatgg gaataccgct tattctgatc
     1861 agaaagaagg gtaaactgcc cggacaaacc tggtcgtgtt catatgctct tgagtacggt
     1921 accgctgaat tggaagttca caaggctgat gtgcaaggcg gtcagcgtat ccttgttatg
     1981 gatgatttaa ttgccaccgg gggaacgctt aatgccgctc gtaatgttct tgaacagggc
     2041 ggagctcggg tagccggct
//
LOCUS       DAOQXQ010000320         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_63116_length_2056_cov_2.485757, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000320 DAOQXQ010000000
VERSION     DAOQXQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63116_length_2056_cov_2.485757"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PKH81_05860"
     CDS             <1..1839
                     /locus_tag="PKH81_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97595.1"
                     /translation="AVDHINAGEWDQARFFAQLGTSYDSTLADFPYIDALALLASDGG
                     RGAAIERLEQALASGRPWRSYDRKSAVLVCARLYAETTRYAQALTLLDEVASYSSADA
                     DYVSALCLYGTGRIREARSLISRAIDRWPFDPRFPRLFLIREKKGSPDDLSLNIASRI
                     TARLSVWENEDRELLLLTVPFEPSADIRKRNIRIYRGMGKNDQQEGQEGLSPLAALLA
                     LEYGLIDETRALEEVFSFSQTGLYRDDLVVLSSLIAGKETRARMVEILRDWNGLLHED
                     SNGDGLIDSRVLYQMGRPYHAYLDPNQDGDIDYELTCSLGKIEQVSIASQGISVRYET
                     YPAVKTITQHGREYVMVPGTFSWAPVSWKGDTFNLGGEPLYTFHYTRSITALTEHALI
                     MSATYYRESSDDGSELRVMLEKGVPLMGERRLGGHVYERILYTRGLPSRASRDENADG
                     HFETIVDYDGQGEIRTISVDNNANSTWEYRETYSPDGSVQKQWDSDENGSWEVSWTLG
                     ADGTEESSWIHPIHALTVTVTVEAGSPRMVRCGESVLAVVRDPVEDLWWIGRMPNEVR
                     DISTRLLEILNHSEPEVVSSILTFNGRRYMTVRTGGFIFVELINAR"
     gene            1829..>2056
                     /locus_tag="PKH81_05865"
     CDS             1829..>2056
                     /locus_tag="PKH81_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97596.1"
                     /translation="MRVNTFFALLFFFLVSCASQKSVEPVVDYSDERSILYEVSKARK
                     MLDENPVLALARARALRDLSRPMEQIDSLYMD"
BASE COUNT          485 a          489 c          536 g          546 t
ORIGIN      
        1 gcagtagatc acatcaatgc cggggaatgg gatcaagccc gttttttcgc acagcttggc
       61 accagctatg attcaaccct ggccgatttt ccttacatag acgctcttgc tcttcttgcc
      121 tccgatggtg gaaggggtgc cgctattgaa cggcttgaac aagcccttgc ctccggccgt
      181 ccatggagaa gttacgacag aaagagcgca gtgcttgtat gtgctcgcct ctatgcggaa
      241 accacccgtt atgcccaggc actcaccctg ttggacgaag ttgcctctta ttcatctgcc
      301 gatgcggatt acgttagcgc tctttgcttg tatggaaccg gtcgcattcg ggaagctcga
      361 tctcttatct cgcgggcaat tgatcgttgg ccctttgatc ctcggttccc cagactgttt
      421 ctaatccgcg aaaagaaagg gtcccctgat gatctgtcct tgaacattgc ttcacggata
      481 accgcccgtt tgagtgtctg ggaaaatgaa gaccgtgagc ttctgttatt aaccgttccc
      541 tttgaaccgt cagcagatat acgaaagcgt aatattcgca tttaccgggg tatgggtaaa
      601 aatgaccaac aggagggcca ggaaggcctt agtccgcttg cggccctgct ggcccttgaa
      661 tacggcttaa tagacgagac gcgtgcgctg gaagaggttt tttcgttttc tcagacgggc
      721 ctgtatcgtg atgatctggt agtcctttcc tccttgattg caggcaagga aacccgggcc
      781 cgtatggtag aaatattgcg ggattggaac ggccttcttc atgaagattc aaatggcgat
      841 ggccttattg actccagagt gctctatcag atggggcgtc cgtatcacgc gtatcttgat
      901 ccaaatcaag atggggatat tgactatgaa cttacctgtt cccttggcaa gatcgaacaa
      961 gtttcgattg catcacaggg gatttccgtg cgctatgaga cctacccagc ggtcaagacg
     1021 ataactcaac atgggcgaga gtatgttatg gttcccggaa ccttttcctg ggcccccgtt
     1081 tcctggaagg gcgatacctt taatcttgga ggcgagccct tatatacctt tcattatacg
     1141 cgatctatca ctgcgcttac cgaacacgct cttatcatga gcgcaacata ttatcgggaa
     1201 agctctgatg acggcagcga attgcgggtt atgcttgaaa aaggcgtgcc cttgatggga
     1261 gagcgacgcc ttggcgggca cgtgtatgaa agaatactgt atacccgggg acttccctcg
     1321 cgtgcctctc gcgatgaaaa tgcagatgga cattttgaaa caatcgtaga ctatgacggg
     1381 caaggtgaga tacgcactat atcagtggat aataatgcca actccacatg ggagtatcgg
     1441 gaaacctatt cacctgatgg gtcggttcaa aaacaatggg acagcgacga aaatggctcg
     1501 tgggaagtca gttggacact tggagccgat ggtacagaag aaagttcatg gattcatccg
     1561 atacatgctc taacggttac cgttaccgtg gaagcaggtt ctccccgaat ggttcgctgt
     1621 ggggaatcgg tacttgcggt ggtgcgcgat cccgttgaag atctgtggtg gattggtcgc
     1681 atgcccaacg aagtgcgcga tatctctacc cgattactcg aaatccttaa ccacagtgaa
     1741 ccagaagttg ttagtagtat acttaccttt aatggcaggc gatatatgac tgtacgtacc
     1801 ggagggttta ttttcgtgga gcttattaat gcgcgttaat actttttttg ctctgttgtt
     1861 ttttttcctt gtgtcctgtg cctcccaaaa atcggttgag ccggtagtag actattccga
     1921 cgagcgaagt attctctacg aggtatccaa ggcaagaaag atgctcgacg aaaatcctgt
     1981 gttggccctt gcccgagctc gtgcactcag agatctatcc agaccgatgg agcaaattga
     2041 cagtctctat atggat
//
LOCUS       DAOQXQ010000321         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_63586_length_2046_cov_3.069312, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000321 DAOQXQ010000000
VERSION     DAOQXQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63586_length_2046_cov_3.069312"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKH81_05870"
     CDS             <1..387
                     /locus_tag="PKH81_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ97597.1"
                     /translation="TGSPSRAMREMDRFMLRVQKNNFIPVIFPEGTRSKNGELGTFHL
                     AGFRRLLDQFPQPVVFCAIDGGWRTSSLGGLLRDLRDGFYRVRIVFVASAPRTKEEQI
                     RVMEAGKEHIRKQLVSWRLMDTVVHT"
     gene            785..1054
                     /gene="rpsT"
                     /locus_tag="PKH81_05875"
     CDS             785..1054
                     /gene="rpsT"
                     /locus_tag="PKH81_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNQ97598.1"
                     /translation="MRNSSAVKRHKQSEVRRVANKAVKSTVRTSAKKYVASVHANDTD
                     TAGTLLRQLVKELDTAARKGIIARNAAARKKSRMQKLYNATFAAK"
     gene            1173..1499
                     /locus_tag="PKH81_05880"
     CDS             1173..1499
                     /locus_tag="PKH81_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ97599.1"
                     /translation="MPDKKRTKSDLVDRIYEKSGQDLKDIRAVVDLFLDEVKTALEEG
                     RSVELRGFGTFEIRERKGRQKARNPKTGEIVSVQDHAVVAFRSGKELKNNVWNVPLSA
                     DTQKGS"
     gene            1499..>2046
                     /locus_tag="PKH81_05885"
     CDS             1499..>2046
                     /locus_tag="PKH81_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNQ97600.1"
                     /translation="MKQSFRRTGGEGTQAFFAGLPVNLALLVVSVLFFALPQPNFLNV
                     RGFPLIAYVAFVPLFILVRRVSFRASFVWGALYGTLCYCLFTYWLAVFHPLAMYIIAI
                     LYCLWCMAVLPLLKAADSLFPRRGFIVQWIIWIGYEYLKTRGFNGYSYGIIGYSQWSF
                     PLISGIASIFGVWGVSALVVFPS"
BASE COUNT          507 a          432 c          505 g          602 t
ORIGIN      
        1 accggtagtc cttcccgggc aatgcgcgag atggatcgtt ttatgcttag agtacaaaaa
       61 aataatttta taccggtaat ttttcccgag ggcacacgct caaaaaacgg tgaattggga
      121 acttttcatc ttgccggttt tcgacgcctt ttagatcaat ttccccaacc agttgttttt
      181 tgtgcgattg atggtggatg gagaacgagt tctttgggcg gattattgcg ggatttacga
      241 gatggttttt atcgggttcg tattgtgttt gtggcgagtg ctcccaggac aaaagaagag
      301 caaatccgtg taatggaagc gggaaaggaa catattcgaa agcagttagt atcttggcgg
      361 ctcatggata ccgtggtcca cacgtaataa aaaaaggaac cctctatagg gttccctttg
      421 ttggccttgt tcaaggcggc tcttatttga agtccttggg acggcgttca gcgcgcttgc
      481 acttgacgca ttccgcaaca ttcttcagtt ttccgctttc aaacagggtt ttcataatta
      541 cttcgccacc gcagggacaa aaaaacttgt gagttgtcac tgaggtacga gagttcttac
      601 ttgctccacc catcttggta tctcctgaaa acaaagatag cacaagaata tagaaataaa
      661 tatgaactgt caagcaattg acggctaaaa ctactggttg cgtttatctt atcatggtat
      721 tgacgtgttt accgtgtttt ggtattcttg taagacagat ttatttgggg ggttcccttt
      781 ggcattgaga aattcgtctg cagttaagag acacaagcag agtgaagtac ggcgggtagc
      841 aaacaaggcc gtgaaaagta ccgtgcgcac cagcgcgaaa aaatatgtcg caagcgttca
      901 tgcaaatgat accgatacgg caggaacact tcttcgccag ctggtcaagg aacttgacac
      961 cgccgcgcgc aagggtatta tcgcccgcaa cgccgccgcg agaaagaagt cgcgcatgca
     1021 gaaactgtat aacgcaactt ttgccgcaaa ataaccgtta atcccaggaa taacgggtcg
     1081 gaaaaccgca agaagcgaac agcttccttg cggttttttt ttcttaacaa gtgttcgttt
     1141 ctatgctacc atgaggtttg agaggttagc gcatgcccga caaaaaaagg actaagagtg
     1201 atctcgtaga caggatttac gaaaaatctg gacaagatct taaggatatt cgggcagttg
     1261 tggatttgtt tcttgatgaa gtcaagacag cccttgaaga aggtagatcc gttgaattgc
     1321 ggggttttgg taccttcgag attcgggaac gaaaaggtcg tcaaaaggcc cgaaatccca
     1381 aaacgggcga aatcgtaagc gtacaagatc atgcagtcgt tgctttccgc tccgggaaag
     1441 aactaaaaaa taacgtctgg aatgtaccct tatccgctga tacacaaaag ggttcctgat
     1501 gaagcaatcc ttccgccgca ccgggggcga aggaactcag gctttttttg ccggacttcc
     1561 tgtgaatctt gcattgctgg tggtatcggt gctttttttt gcacttccac aaccaaattt
     1621 ccttaatgtt cgcggttttc cccttatagc ctatgttgcc tttgttcccc tgtttatttt
     1681 ggttcgtcgc gtgtccttca gagcctcatt tgtttgggga gctttgtatg gaacgctttg
     1741 ttattgtctg tttacctatt ggctggcggt attccatccc ctggcaatgt atattatcgc
     1801 cattctttat tgtctgtggt gtatggctgt tcttcccctg cttaaggccg ctgatagtct
     1861 atttccccgg cgtggtttta tcgtgcaatg gattatctgg attgggtatg aataccttaa
     1921 gacccgtggg ttcaacggct attcatatgg aataatcgga tattctcaat ggtcatttcc
     1981 cttgattagc ggaatcgcat ctatttttgg ggtgtggggg gtttctgccc tggtagtgtt
     2041 tccttc
//
LOCUS       DAOQXQ010000322         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_63882_length_2040_cov_2.582368, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000322 DAOQXQ010000000
VERSION     DAOQXQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63882_length_2040_cov_2.582368"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..968)
                     /locus_tag="PKH81_05890"
     CDS             complement(69..968)
                     /locus_tag="PKH81_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97601.1"
                     /translation="MKTHGVAELTVRLAGAAYPVALPDEGTVLEVAITGQSADGRFRI
                     RVGSIALLASAPDGSWRPGDTFLATIRYRNGTLFLVPNPVQPRVSDVLPSLGLPQHPV
                     STFLVQFFKAMNRRLDGSILRSLARQALSHTDRPLRAAEALALLESASLIPSEEAVEA
                     LSDIIEGKGYETEAKALAYKRLTSLINHCHAHSHRWMCIPFDKEIEGKRLHGSIRVLY
                     DEENLRVVQTLVTVDDESRLWDFSLDSQSCLFSVYPPPDPVVFGRLRVYLEEELRMAG
                     ISKVVYHDGIHDSTPIPSVDLEA"
     gene            complement(1019..1384)
                     /gene="rplS"
                     /locus_tag="PKH81_05895"
     CDS             complement(1019..1384)
                     /gene="rplS"
                     /locus_tag="PKH81_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNQ97602.1"
                     /translation="MELIHTIEEGQKKAEVPAFKVGDTVKVHFKIIEGKTERIQIYEG
                     LVICFKNSGIGRTFTVRKNSYGVGVERVFPLHSPRIANVELLRPGKVRRAKLYYIREK
                     IGKAAKIKTLIVKKAEHKD"
     gene            complement(1362..>2040)
                     /gene="trmD"
                     /locus_tag="PKH81_05900"
     CDS             complement(1362..>2040)
                     /gene="trmD"
                     /locus_tag="PKH81_05900"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNQ97603.1"
                     /translation="FALDRHKTCDDKPYGGVLGAKVPGMLMLAEPLSRALESVGVMAK
                     DQSSRVSTGRRVIYVSPSGRPLDQNLVTELSGETELVFVCGRYEGIDQRIIDLYVDDE
                     ISIGDYVMSSGELAATVIIDAVYRLIDGVISSESLEDESFSGGLLEYPQYTRPEIFRD
                     LKVPQVLLSGHHEKIRRWRLRKRVEKTLSVRPDLLTKLRASCSLTGELDKIIKELENG
                     NGTDPHD"
BASE COUNT          552 a          515 c          481 g          492 t
ORIGIN      
        1 ggaggcaaca ataaccgggg cgaccatctg ttgagtataa cctaaggcaa ccgcagtgtt
       61 tcgtttcatt aggcctccag gtctaccgaa ggtattggtg tagaatcatg aattccatca
      121 tggtatacaa cctttgaaat acctgccatc cgtaactctt cttctaaata tacacgtaaa
      181 cgaccaaaaa caacagggtc aggtggagga tagacgctaa agaggcagga ttgactgtca
      241 agtgagaaat cccacaaacg gctttcatcg tcgacggtga ctaaggtttg aacaacccga
      301 aggttctcct cgtcatataa tacgcgaatt gatccgtgaa gccttttccc ctctatctct
      361 ttatcaaagg ggatacacat ccaacgatgc gaatgggcat gacaatgatt gattaacgat
      421 gtaagccttt tataagcaag ggcctttgct tcggtttcat aacccttccc ttctattatg
      481 tccgataatg cttcaacagc ctcttctgag gggatcagag atgcgctttc caaaagcgcg
      541 agagcctctg ctgcacgcaa gggcctgtct gtatgggaga gggcttgtcg cgccaggcta
      601 cgaaggatag aaccgtcaag tcgtcgattc atcgccttaa aaaattgtac taaaaaagtt
      661 gatactggat gctgtggtaa accaagagat ggaagtacat ccgaaacacg aggttgtacg
      721 ggattaggca ctaaaaaaag ggtgccatta cgatagcgta ttgtagcaag gaaggtatcg
      781 cccgggcgcc aagaaccatc aggagcggat gcaagaaggg caattgaccc tacccgaatg
      841 cgaaagcgcc catcggcaga ttggccggta atggcaactt ccaggacagt tccctcatca
      901 ggaagcgcaa caggataggc ggccccggca aggcgaacgg ttagctctgc aaccccatgg
      961 gttttcacac acaatccccc ataaaaaaaa gccggacgct catggtccgg cctctttctt
     1021 agtccttatg ttctgccttt ttaacgatca aggtcttgat cttggcagcc ttaccaatct
     1081 tctcgcggat ataatagagc ttggcgcggc gtaccttacc aggtcgcaac aactcgacgt
     1141 ttgcgattcg gggtgaatgc agggggaata cacgttctac accaactccg taggagttct
     1201 tgcgtacggt aaaggttcgg ccaataccgg agttcttgaa gcaaataaca aggccttcgt
     1261 agatctgaat acgctctgtt ttaccttcaa taatcttaaa gtgtacttta acggtatctc
     1321 caactttaaa cgccgggacc tctgcctttt tttgtccttc ctcaatcgtg tggatcagtt
     1381 ccattcccat tttcaagctc cttgataatt ttgtccagtt ccccggttaa ggagcaggac
     1441 gcccgcagct ttgtaagcaa atcgggcctg acgctcagcg ttttctcgac gcgctttcgc
     1501 aaacgccagc gacgtatttt ttcgtggtgc cccgaaagaa gcacctgcgg aaccttaagg
     1561 tccctaaata tttcaggtct tgtatactgc ggatactcaa gaagtccgcc ggaaaaactt
     1621 tcgtcttcca gggattcact tgaaataacc ccatcaatca aacgatagac cgcatcgata
     1681 ataaccgtag cagcgagctc gccagacgac ataacatagt cgccgatgga aatctcgtca
     1741 tcaacgtata aatcgataat ccgctgatca attccttcgt aacggccgca aacgaacacc
     1801 aattcggttt cgccagaaag ctcggtaacc aggttttgat caagaggcct tccagacggg
     1861 ctaacgtaga taacgcgtcg accggtggaa accctgcttg attgatcctt tgccatcacg
     1921 ccaacggatt caagggcgcg ggaaaggggt tcagctagca taagcattcc ggggaccttg
     1981 gccccaagaa cgccgccata gggtttgtca tcgcaggttt tatgccgatc caaggcaaaa
//
LOCUS       DAOQXQ010000323         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_63936_length_2039_cov_2.604335, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000323 DAOQXQ010000000
VERSION     DAOQXQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63936_length_2039_cov_2.604335"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKH81_05905"
     CDS             <1..598
                     /locus_tag="PKH81_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNQ97604.1"
                     /translation="IMIFALSAAVSERFMNDWQDIVSERIYRCVCERVKGRDDLEDAA
                     TIDLPIAYNRQHVGFVPSGKEPKLLKAAERAVTRYKVLARGERYDLLECELETGRKNQ
                     IRIHLAHLGHPVVGDEVYGLQKSPPSGLSRLALHARVIAFRHPFTADLLRFEKSEPAE
                     FLSVTMKAKAPRPVRNRESASCPLAARSNLRRPGKKKR"
     gene            complement(595..1365)
                     /locus_tag="PKH81_05910"
     CDS             complement(595..1365)
                     /locus_tag="PKH81_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97605.1"
                     /translation="MKKNLYDGWDNVLTLLAINALLLVVNALFLFCALSLSSHQALAW
                     LLLSLAWLVTGGLLAAVSAECYRVASFKSFSWKSLVSHLKTESIYGILCAALSLLAAL
                     ILLVSVPWYARWGNGIGLAFALVLIWIALVVILSLQWFYPIRSQLKVSFIRSVQKCFV
                     IFFDNPGLSLFMALYSLLCIFISLITLFLLPGFSGLILAYNEAFRLLLYKYDWAEAHP
                     LMDWRVARKQVPWDDLLAHDEETLGDRSFKHFIFPWKN"
     gene            1426..>2039
                     /locus_tag="PKH81_05915"
     CDS             1426..>2039
                     /locus_tag="PKH81_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ97606.1"
                     /translation="MKHSVYRIMVSAIALTLLSALPLFSAGRTEPSTQELSIAVFVPG
                     VVSGSPVYEMLVAGAQNAVNEAITAGTKVSLTVIEAGTRQADWGTKMVSTAAEGGYSL
                     IITSNPAMPEIIEPITLLFPKQDFLVFDAKHEGNPAITTFRYNQREQAYISGYMAALV
                     SSSSMTHANKAKKIGLIAGQEYPAMTEIILPAYTEGARAVDPSF"
BASE COUNT          601 a          523 c          495 g          420 t
ORIGIN      
        1 aattatgata tttgccctgt ccgcggcggt atccgaacgt tttatgaatg actggcaaga
       61 cattgtgagc gaacgaatct atcgctgtgt gtgcgagcgg gttaagggga gagatgacct
      121 tgaggacgcg gcaacgatcg atcttccaat tgcctataac cggcaacacg tcggctttgt
      181 tccctcggga aaggagccaa agctcctgaa agcggcggaa cgggcggtaa ccaggtacaa
      241 ggtgcttgcc cggggagagc gatacgattt acttgaatgc gagttggaaa caggccgaaa
      301 aaaccaaata cgaattcatc ttgcccatct gggccatcct gtagtggggg atgaggtgta
      361 tggactacag aagtcgccac cttcgggcct ctcccgtctt gccctgcatg cccgcgttat
      421 tgcctttcgc catcccttta ccgcagacct gcttagattc gaaaagagcg aaccggcaga
      481 gttcctctcg gtaacaatga aggcaaaagc tccccgtccg gtccgtaatc gcgaaagtgc
      541 atcgtgcccc cttgccgcgc gatcaaacct gcgacggcca gggaaaaaga aacgttagtt
      601 tttccagggg aagataaaat gcttaaagct gcgatccccc agggtttctt cgtcatgggc
      661 aagtaaatca tcccaaggca cctgttttct cgcgacgcgc caatccataa gcggatgagc
      721 ctcggcccaa tcatacttgt acagtaaaag ccggaaggct tcgttatacg caaggataag
      781 accggaaaaa ccgggcaaga ggaaaagggt tatcagggaa ataaaaatgc acaacagtga
      841 atagagggcc ataaaaagag atagccccgg attgtcaaaa aaaatcacaa agcatttttg
      901 gaccgatcgg ataaacgaca ccttaagctg cgaacgaatg ggataaaacc attggagcga
      961 cagtattacc accaaggcaa tccaaatgag cacaagggca aaggccaaac cgataccatt
     1021 tccccagcgc gcataccagg gcaccgacac caaaaggatc agagccgcaa gcaaggaaag
     1081 ggctgcgcat aagattccat atatactttc ggttttaaga tgagagacaa gagacttcca
     1141 cgagaacgac ttaaaagacg ccacccgata acattcggcc gatactgcgg ctaaaaggcc
     1201 tcccgttacc aaccaggcaa gagaaagcaa aagccaggcc agggcctgat gtgacgaaag
     1261 ggaaagcgcg caaaacaaaa aaagagcatt gaccaccaat aaaagcgcgt taatcgctaa
     1321 taaggtcaac acattatccc agccatcata gaggttcttt ttcaaaaaaa atccggtcat
     1381 gtttctacgg tagcatattc cgcgtgtatt tactatattt tattcatgaa acattctgta
     1441 tatagaatca tggtatctgc catcgcatta accctactaa gcgctcttcc gctgttttca
     1501 gctggccgca cagagccatc tactcaggaa ctatcgattg ccgtttttgt acccggcgtg
     1561 gtttccggaa gcccggtgta cgagatgctt gtggcaggcg cgcaaaacgc agtgaacgaa
     1621 gccattaccg ccggtacaaa ggtaagcctt accgttatag aagcgggtac ccgacaagct
     1681 gactggggaa ccaagatggt ttccaccgca gcagagggcg gctattccct tatcatcacc
     1741 tccaatccag caatgcccga gataatagag ccaataaccc tgttatttcc caagcaggac
     1801 tttctggtct ttgacgccaa acatgaggga aatccagcca tcacgacctt ccgctacaat
     1861 cagcgagagc aagcctatat ttcaggctac atggcagccc tggtaagctc aagctcaatg
     1921 acccatgcaa acaaggctaa aaaaattggc ttaatcgccg ggcaggaata ccctgccatg
     1981 acggagatta tccttcccgc ctatacagaa ggagcccggg cagttgaccc atcttttga
//
LOCUS       DAOQXQ010000324         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64087_length_2036_cov_2.467945, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000324 DAOQXQ010000000
VERSION     DAOQXQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64087_length_2036_cov_2.467945"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKH81_05920"
     CDS             complement(<1..732)
                     /locus_tag="PKH81_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNQ97607.1"
                     /translation="MKQRGLFDEDERLLELSAIGDPLEKLNNVINWSIFRPTLNTALL
                     KEQKGPGGRPPYDYIMMFKILILQRLYNISDDQTEYQIKDRLSFMRFLGLSLKDTIPD
                     AKTIWHFREQLVKAKVSEKLFKKFLRELENKNLVTRQGSIIDATFVDVPKQRNTKDEN
                     DSFKEGNIPEEWKEPKNKAKLSQKDTDARWTKKNDETHYGYKAHVKADSKSKLILKYT
                     VTAASIHDSQELPNLVDGKDKALYAD"
     gene            962..>2036
                     /gene="leuS"
                     /locus_tag="PKH81_05925"
     CDS             962..>2036
                     /gene="leuS"
                     /locus_tag="PKH81_05925"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ97608.1"
                     /translation="MAKYPFETIEPKWQAFWEEHKTFRAVEDPSKSKDKRAYVLDMFP
                     YPSAAGLHVGHPEGYTATDIWCRYLRMNGYSVLHPMGFDSFGLPAENYAIKTGTHPRT
                     TTLANINKFRQQIKAFGFSYDWDREISTCDDDYYHWTQWIFLQLFKRGLAYEAETPIN
                     WCPSCKTGLANEEVKEGKCDRCGTQTSRKNIRQWILKITAYAERLLEDLDGLDWSESI
                     KAMQRNWIGRSEGAEVTFTVDGSKEEILIYTTRADTLFGATYMVLSPEHPLVPTITRS
                     EQAAAVNAYRDAAAKKSDLERTDLAKEKTGVPTGAYAINPVNGQKIPIWISDYVLISY
                     GTGAIMAVPAHDERDWDFAKAFSL"
BASE COUNT          545 a          494 c          418 g          579 t
ORIGIN      
        1 atcagcatac aaagctttgt cttttccatc aacaagattg gggagttctt ggctgtcatg
       61 aatgcttgct gctgttactg tatattttag tattaacttg cttttactat ctgcttttac
      121 atgagcttta tatccatagt gagtttcatc attcttcttt gtccagcggg catcggtgtc
      181 tttctgtgac aattttgcct tgtttttagg ctccttccat tcttcaggaa tatttccttc
      241 tttaaaacta tcgttttcgt ctttggtatt tctttgcttt ggtacatcaa caaatgtcgc
      301 gtcgataata ctaccttgtc gagtgacaag attcttgttt tctaattctc ttaggaactt
      361 cttaaagagc ttttcactaa cctttgcttt gacgagttgt tctcgaaaat gccagatagt
      421 ctttgcgtca ggaatagtat cttttagact taaaccgaga aatctcataa agctcagacg
      481 atccttaatc tgatattctg tctgatcgtc actgatgtta tacaaccttt gcaaaataag
      541 tatcttaaac atcatgatgt aatcatacgg tggtctcccg cctggacctt tttgctcttt
      601 gagtaacgcc gtgttcaatg ttggtcgaaa tattgaccaa ttgataacat tgtttaattt
      661 ctccagtgga tcaccaatcg cgctgagctc aagcaatctt tcatcttcat caaacaaacc
      721 gcgctgtttc atgttttcta ttttatcata tttttaaaga gtttttcgag gtgcccataa
      781 agcagcaata aaaacggcac acagtgccac aatcttgtat gtacgcatag ccagtcctcc
      841 tgtatggaat cagtatacgc taactctggt tttcttcaag ccttttccaa gcttgctaaa
      901 agaagagtgt ttctatagaa taatttcact tacaacattt ttttggggag taatagcggt
      961 tatggcaaaa tatccttttg agacaattga acccaagtgg caagcatttt gggaggaaca
     1021 caagaccttc cgcgcggtag aagatccatc caaaagcaag gacaagcggg cgtatgtcct
     1081 tgatatgttc ccctacccct ccgccgccgg cctgcatgtt ggacatccgg agggctacac
     1141 cgctaccgac atttggtgcc gctatcttcg catgaacggt tattcggtcc tccatcccat
     1201 gggatttgac tcctttggct taccggccga aaactacgct ataaaaaccg gaactcaccc
     1261 ccgaaccaca acactagcaa acatcaataa attccgtcag caaataaagg ctttcggttt
     1321 ctcctatgat tgggatcgcg aaatatccac ctgcgacgac gactactacc attggactca
     1381 gtggatattc ttgcaattat tcaagcgcgg actggcgtac gaggcggaaa ccccaatcaa
     1441 ttggtgtccc tcctgtaaaa caggccttgc aaacgaagaa gttaaagaag gcaaatgcga
     1501 tcggtgcgga acccaaacct cacgaaaaaa catcaggcaa tggatactca agattaccgc
     1561 ctacgcagaa cgccttctgg aagacctgga tggccttgat tggagcgaat ccatcaaggc
     1621 aatgcaaaga aactggattg ggcgtagcga aggagccgag gttaccttta ccgttgacgg
     1681 ttcaaaagaa gaaattctga tttacaccac tcgggcagac accctttttg gggcgaccta
     1741 catggtgctt tcgccagaac atccccttgt accaaccatc actcgctccg aacaggccgc
     1801 agcggttaat gcgtaccggg acgctgccgc aaaaaaaagc gaccttgagc gcaccgattt
     1861 ggcaaaagaa aaaaccggcg tccctaccgg agcatacgcg ataaacccgg taaacggaca
     1921 gaaaataccc atatggatct cggattatgt gcttatctca tacggaaccg gcgccattat
     1981 ggccgttccc gcccacgacg agcgggactg ggactttgcc aaggctttct cgcttc
//
LOCUS       DAOQXQ010000325         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64182_length_2034_cov_2.212734, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000325 DAOQXQ010000000
VERSION     DAOQXQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64182_length_2034_cov_2.212734"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="frr"
                     /locus_tag="PKH81_05930"
     CDS             complement(<1..461)
                     /gene="frr"
                     /locus_tag="PKH81_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ97609.1"
                     /translation="MSEAITDNIERMKKSVSALKDDLNTIRTGRASASLFDKIRVDYY
                     GDKSPLTQVATVSIPEARLVVIQPWDKGLIGEIEKAIQKSELSLNPSNDGKVIRIAIP
                     PLTEERRKELVKQAKASAEQSRVAVRNIRRDGNEELKKLQKDGKMTEDDLKV"
     gene            complement(549..1388)
                     /gene="tsf"
                     /locus_tag="PKH81_05935"
     CDS             complement(549..1388)
                     /gene="tsf"
                     /locus_tag="PKH81_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNQ97610.1"
                     /translation="MDIKASEVKALREKTGAGMMECKNALIASNGDAAQAEKILKEKG
                     LAAVEKRAGRATTEGLVLIKSDGKKASMIELTCETDFVAKNDDFIALADQILEASFTN
                     SYNEVTAELSSKVLELATKIRENMALTRIVTLTAGPDEYIARYIHSDKKTGVLIMLKG
                     DKGDVVSNKAVQDFAYDCCLHVAAFTPLYINSEAVDKNYIQEQTDIFTKQVAELDKPE
                     QVKAGIVQGKIKKHLAEICFLEQPFVKDDKLSVSKKMDEVGASAGGKLSLSRMVLFQL
                     GVN"
     gene            complement(1411..>2034)
                     /gene="rpsB"
                     /locus_tag="PKH81_05940"
     CDS             complement(1411..>2034)
                     /gene="rpsB"
                     /locus_tag="PKH81_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNQ97611.1"
                     /translation="VKMPYVTESWPGGMLTNFSTIKKSLLRLKKIEKMEIDGTFESLT
                     KKEIAALQKEKTKLEKNLGGIKEMKELPGIIFVIDTRKEAIGVAEARRMGIPIVAVVD
                     TNCNPEGIDYPIPGNDDAIRAISLFTQIIANAVIEADNENGLKIIENLQDEDDEDALN
                     ASVPSKDEEVIEDYSNYTPETVASADTPAADAGDSALVDEDKLYDDK"
BASE COUNT          457 a          461 c          465 g          651 t
ORIGIN      
        1 actttcagat catcctcggt catcttgcca tctttttgga gctttttaag ctcttcattt
       61 ccgtctctac ggatattgcg aacggcaaca cggctttgtt ctgctgaagc tttagcttgt
      121 ttgaccagtt cttttcgtct ttcttctgtt aggggcggta tagcaatgcg gattactttt
      181 ccatcattgg atggattaag cgaaagttca gatttttgga tagctttttc aatttcgccg
      241 ataagcccct tgtcccaggg ctgaataacg accaggcgtg cttccgggat tgaaacggtg
      301 gcaacctgtg taagagggga cttgtcacca taatagtcta ctcgtatttt atcaaaaaga
      361 gacgctgaag cgcgtcctgt tcgaattgta ttcaggtcat cttttagggc ggatacggat
      421 tttttcatcc gttcaatatt gtcggtaatc gcttcggaca tgtgattctc cttctctatg
      481 gtcctggaat ggaaaaagct gtccgaaccg tttcggacag ccttttatca caagattact
      541 cagggttatt agttaacacc aagctggaat agaaccatac gggaaagcga cagtttgccg
      601 ccagccgatg cgccaacctc atccattttt ttggaaacag agagcttgtc gtcctttaca
      661 aagggttgtt ccaggaaaca aatttcagca aggtgttttt taatctttcc ttgaacgatt
      721 ccggccttta cctgttcggg tttgtcaagt tctgcaacct gcttggtaaa gatatcggtc
      781 tgttcctgga tgtagttctt atctacggcc tctgagttga tgtagagggg cgtaaatgct
      841 gcaacatgga ggcaacagtc gtaggcaaaa tcttgaactg ccttgttgct taccacatcg
      901 cccttgtctc ccttaagcat tatcaacacg ccggtctttt tgtcggagtg gatatatcgt
      961 gcgatatatt catccggacc tgcggtaaga gttacaatac gggtaagggc catgttttcg
     1021 cggatcttgg tggcaagttc cagaaccttg ctagagagtt ctgcggttac ttcgttgtaa
     1081 gagttggtga agcttgcttc cagaatttga tctgcaagtg cgataaaatc atcgtttttg
     1141 gcgacgaaat cagtttcgca tgtcagttca atcattgaag ctttttttcc atcactcttt
     1201 ataagtacga gtccttcggt tgtagcccgt ccagctcgtt tttcaacggc agcaaggccc
     1261 ttttccttaa gaatcttttc ggcttgcgca gcgtctccat tggaagcaat gagggcgttt
     1321 ttgcattcca tcatccctgc accggttttc tcgcgcaggg ccttaacttc agatgctttg
     1381 atatccatga tatggtacct cttctaatcg ttatttgtcg tcgtacagct tgtcttcgtc
     1441 caccagtgcg ctgtcgcctg catcggcagc aggagtatcc gcagaagcaa cagtctcggg
     1501 agtgtaattg ctgtaatcct cgataacttc ttcatccttc gaaggaacag aagcattaag
     1561 tgcatcttca tcgtcctcat cctggaggtt ttcgataatc ttaagaccat tttcgttgtc
     1621 agcttcgata accgcgttgg cgatgatctg ggtgaacaag gaaatagcac gaatggcgtc
     1681 atcgttaccg gggattggat agtctatacc ttcgggatta cagttggtat ctacaacggc
     1741 aactatagga atgcccatgc ggcgagcttc tgcaacgcca atagcttcct tgcgtgtatc
     1801 aatcacaaag ataatgccgg gaagctcttt catttccttg ataccaccaa ggtttttttc
     1861 cagcttcgtt ttttctttct ggagagcggc tatttctttt ttggtgagac tttcgaatgt
     1921 gccatcaatt tccatctttt caattttctt caggcgaaga aggctctttt tgatggtgga
     1981 aaagttggta agcataccgc ccggccagct ttcagttacg taaggcattt tcac
//
LOCUS       DAOQXQ010000326         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64214_length_2033_cov_3.067240, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000326 DAOQXQ010000000
VERSION     DAOQXQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64214_length_2033_cov_3.067240"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKH81_05945"
     CDS             complement(<1..429)
                     /locus_tag="PKH81_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97612.1"
                     /translation="MLNTLKKISQSQLIIPLFALFLVIVFNVFRDLSFFSISVIRNNY
                     GNLVLSGNIISILHGASELAVLAIAMTLVTSATKGQDISVGALAAIAGSVFVRVLRAN
                     TITPQIIIVGFLIACVVAILFALFTGMLVSFFNIQPMIASL"
     gene            complement(422..1942)
                     /locus_tag="PKH81_05950"
     CDS             complement(422..1942)
                     /locus_tag="PKH81_05950"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNQ97613.1"
                     /translation="MNNDIVLTMRGICKSFPGVRALHNVDFTLCKGEIHALMGENGAG
                     KSTLVKVITGVYTKDLGHITIAGSDKEVVIKSPEDAQKAGISTVYQEITLCPNLTVAE
                     NIYIGRGSYRFVNARSQEKKATEVLQRLNIPTTATRQLGTCSLAVQQMVAIARAIDMD
                     CKVLILDEPTSSLDEQEVALLFSLMRDLKARGVGIIFITHFLEQVYEVCDKITVLRNG
                     ELIGEYQVQDLPRVQLISKMMGKELDELSELQNRKNIDIDDGIPSVYEAEELSSSEGT
                     YPFDFAIRKGEINGFTGLLGSGRSECIRAIFGADRITGGKVRVNGKTVKIAKPRDAMA
                     CGIGYLPEDRKLDGILADLSVRENILLALQVMKGFFKPFSKAEAESFAQKYITLLGIK
                     TASADTPIKSLSGGNQQKAILARWLLTNPQYLLLDEPTRGIDIGTKVEIQKLVLSLAE
                     QGVSVTFISSEIEEMLNTCSRLIVMRDRKIVGELKGQDMTQTRIMHTIAGEAAQHA"
BASE COUNT          547 a          476 c          435 g          575 t
ORIGIN      
        1 cagggaagcg atcattggct gaatgttaaa aaaagaaacg agcataccgg taaacaaggc
       61 aaagaggatt gcaaccacac acgcgataag aaaaccaaca atgataatct ggggagttat
      121 ggtattagcc ctgagtacac gaacgaacac gctgcccgca atggcagcaa gagctcctac
      181 gctgatatct tgtcctttgg ttgccgatgt caccagggtc atggcaatgg ctaatacagc
      241 caactcggaa gcgccatgga gtatgctaat tatattgccg gaaagaacca ggttgccata
      301 gttgtttctt ataacactta tagaaaaaaa actgaggtca cgaaatacat taaatacgat
      361 taccaaaaac agtgcaaaca gaggaattat cagttgcgac tgtgatattt ttttaagtgt
      421 attaagcatg ttgtgcagcc tcccccgcaa tggtgtgcat aatccgtgtc tgagtcatat
      481 cctgtccttt aagctcacca acaatcttcc gatcacgcat aacaataagc cgtgaacagg
      541 tattaagcat ctcctctatt tcggaggaaa tgaaggtaac gctcacccct tgttccgcaa
      601 gagagagcac cagtttctga atctctacct tagttccaat atcaattccc cgtgttggtt
      661 catccagtag tagatactgt ggattggtta atagccaacg tgcaagaatc gctttttgtt
      721 ggttccctcc cgaaagagat tttataggtg tgtcagcaga tgccgttttg attcccaata
      781 acgtgatata tttctgggca aagctctctg cctcggcttt ggagaatggt ttaaaaaagc
      841 ctttcattac ctggagggct aacaggatgt tttcgcgcac ggataaatcc gctaggatac
      901 catcgagttt tctgtcttcg ggtaaatagc caattccaca agccatggcg tcgcgaggct
      961 tggcgatttt tacggttttc ccatttaccc gtaccttgcc gccagtaata cgatctgcac
     1021 caaagatggc ccgtatgcat tcgcttcttc ccgaacccag caagccggta aaaccgttta
     1081 tttctccctt acgaatcgca aaatcaaatg gataggtacc ctctgagctg gaaagttctt
     1141 ccgcctcgta caccgagggg atcccatcat ctatatcaat attttttctg ttttgtaatt
     1201 cggaaagttc atcaagttct tttcccatca ttttggatat cagttgaacc cgaggcaaat
     1261 cctgaacctg atattctcct atcagttcac cgtttcgtaa tacggttatc ttgtcgcata
     1321 cctcgtacac ctgttccaaa aagtgtgtga taaagataat gccaacacca cgagctttta
     1381 ggtcgcgcat aagagaaaat aaaagagcta cttcctgttc gtcgagggat gaggttggct
     1441 catccaggat aaggaccttg caatccatat cgattgcgcg agcgatagca accatttgct
     1501 gtaccgcgag tgaacacgtt cctagttgtc gcgttgccgt tgttgggatg tttagtcgtt
     1561 gaagaacctc ggtcgccttt ttttcctggc tacgggcgtt aacaaatcga tacgagccac
     1621 gaccaatata tatattctct gccacggtaa gatttgggca gagtgttatt tcctgataga
     1681 ctgtacttat tccggctttt tgagcgtctt ccggtgattt gattactacc tctttatcgg
     1741 agccggcaat tgttatgtga cccaggtctt tggtatacac tccggtaatc acctttacaa
     1801 gggtagactt accggcgcca ttttctccca tcaatgcatg aatctcaccc ttgcacaggg
     1861 taaaatccac attatgtaaa gcccgtacac ccggaaatga tttacagatt ccgcgcattg
     1921 ttaaaacgat gtcgttattc attggatata caccacctgt ttgcgttgag acgtaaacag
     1981 caagggccat cccgcaggac ggcctcttgc tgtacaatag cttttactta gtt
//
LOCUS       DAOQXQ010000327         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64490_length_2028_cov_2.739990, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000327 DAOQXQ010000000
VERSION     DAOQXQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64490_length_2028_cov_2.739990"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKH81_05955"
     CDS             <1..798
                     /locus_tag="PKH81_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ97614.1"
                     /translation="TPIYVSDIDGRIDTVNRAWEKTFGFQHTQVTGRSFYDIFTRETA
                     DRINSTNEKLLQMGTSTEFEESINCPTGLHYFHTVKFPLSDETGKKVAVGVISVDVTT
                     RKNIEKELKKHETELRIKSQQLEEINTALRVLLRQREEDQRELEERIVSNVRELVLPY
                     VDKLKGMHLSATQMSYLEIVETHLNDIITPFLRQMVSDYPHMTAKELKVATLVKEGKT
                     NKEMADLMNVSVNAIEIHRYNLRKKLDLQNKKINLRSYLLSLKKLAS"
BASE COUNT          594 a          449 c          482 g          503 t
ORIGIN      
        1 acccctatct atgtgtccga catcgatggt cgtatcgaca cggtgaatcg ggcgtgggag
       61 aaaacctttg gtttccaaca cacacaggta actggcaggt ctttctatga tatctttacc
      121 cgagaaacag ctgaccggat aaatagtact aacgaaaaac ttttacagat gggtacttca
      181 actgaatttg aagagtcgat taattgtcct accggactac attatttcca tacggtgaaa
      241 ttccctttat ctgatgaaac aggaaaaaag gtcgctgtgg gtgtaatttc agttgatgta
      301 acgactcgga aaaatattga aaaagagttg aaaaaacatg aaaccgagtt aagaataaag
      361 tcccaacaac tggaggaaat aaatacggca ctaagagttt tgctgaggca gcgcgaggaa
      421 gatcaaagag agttggaaga aaggatcgta tccaacgtca gagaactggt tctcccttac
      481 gtcgataagt tgaaaggaat gcatctgagc gcgacccaga tgagctatct ggagatcgtc
      541 gaaacgcatt taaatgatat cattacgccc tttctaagac agatggtttc tgattatccc
      601 catatgacgg ccaaagaact taaggtggca accctcgtga aagaaggtaa aacaaacaag
      661 gaaatggctg atctcatgaa tgtatcggta aatgccatag agatccacag gtacaatcta
      721 aggaaaaagc tggacctgca aaacaaaaag ataaatttga ggtcatacct tttatcccta
      781 aaaaaactgg cttcgtaaaa tctatccgtc gttgtcaagt tttttctctt ttgacccgac
      841 ttagcggatt cgaccctcaa aaatgcccgc caattgcatt tttctgacca tgtcattacg
      901 atagattatg agtgtattat accccccaaa accgcctgta gtgaagacct acaccctttt
      961 caggaataaa tgtcaacggt atacttggag tgaattcccc cgttttacaa aacaaaaaag
     1021 gatggcaaag aacaccggta cgggagcgtt atggataaca gccaggcagc gggagcttgt
     1081 gttgtcgaga agcagatggt ctacctctga gctgcgccgc ccacggtata gggtacattt
     1141 gactggcttg tacattgtat gaacaaccgg gcctcgacac cttctggggg gaacgcccgc
     1201 tgccctcccg caagaagacg ctatgggacc tcatcggcca ggctctttgc tgttactgtt
     1261 ttatttatcc tggcagcgaa tggcgtcact acaggcactg gtatgagaca aacgccatgg
     1321 ccgatctcct tggctccggg tttgaactga cgaaatgcac aaactgtatg cgtgtctgga
     1381 tcggatgctt ccccatacgt cagcctcccc gactatctga tgcagcgatg gaaagacctg
     1441 ttcaatttca atgtgaagtt tgaagtcctt ccccgcgacc tgaccagtat atgccgttag
     1501 aacaattccc cctgttttct atcttgcagg gatgtccaaa ggtctgctat gctgatacgc
     1561 gaaggcgctg accgatcaat catggcataa tgtccgtaat agtgtcgatt tcaaacttct
     1621 tcaccaggat agcgaggtct gcatactggc gcaatgcaga gaccgcgtgc atagggtgtg
     1681 tccatcagac gacaacaaca gaaaaaattc tggaaacgac tgcgctagct acagaatatg
     1741 aatctcgcca aagatcattt acttctgaga ctcggggcag ctctggtaag gctttatatt
     1801 gccgagatgt gggagattta cactcagcta agtggagtta gtgcttcgtg cttaggattt
     1861 agaatttcct attcttacaa aatgacatcg tatcctggat aaacagaaaa aaagcatttg
     1921 ctgaaagtat atcagggaac gggatcggcg gcgcttgaga agaaggcctt cagcctttga
     1981 agccagcggg cagggttgcg gagcttgcgc caggcaagtt gcgcggca
//
LOCUS       DAOQXQ010000328         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64855_length_2022_cov_2.364514, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000328 DAOQXQ010000000
VERSION     DAOQXQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64855_length_2022_cov_2.364514"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKH81_05960"
     CDS             complement(<1..797)
                     /locus_tag="PKH81_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97615.1"
                     /translation="MRVEDVLAWREALTCLSDQHFFDLMRMYLGQIKTPFNKQRLIEE
                     LSAFLRKSGTRERLVSALDNLDYLVLSAIQVLPVPTQQKIVNVLAPSLSFPDVYERIL
                     NLEERLVIYREADGGDAKRGKDFSSRRYAINPHLLDALPDFDPLSVLTAPSFPSQATH
                     AVLPVDDMALAGLYSFFLHSDESPRNDGSLRKKTIAELLSVFPHFPRDEQFPDMVTVA
                     LRNLSLLVVRDGSLLPDAARWKAFTETAPLERLAYVIASVNGRYDRQT"
     gene            complement(788..>2022)
                     /locus_tag="PKH81_05965"
     CDS             complement(788..>2022)
                     /locus_tag="PKH81_05965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ97616.1"
                     /translation="GWPVKDEAPLKEGEPLSLSLRSACVSGKPLVIRDYQKNAARALV
                     GDKGPGTGFGTIVLPCGSGKTVVGMEVMTLLQTSTLILTTNIAAVHQWRDELLDKTSL
                     TREEIGEYSGDLKEIRPVTIATYQILTWRPNKEEDFPHFRIFRSRAWGLIIYDEVHLL
                     PAPVFRVTAEIQTVRRVGLTATLVREDGCEGDVFSLVGPKRYDVPWKELEGQGWIATA
                     SCTEFRVDMDSERELSYAVAGQREKYRIASENPAKIPAVIALLEKHREDSILIIGQYI
                     KQLETIARATGAPLITGKTPNTERDRIYGEFRSGKIKTLVVSKVANFAIDLPDASVAI
                     QISGTFGSRQEEAQRLGRILRPKEKDSHFYTIVTSRTVEEDFAANRQKFLAEQGYEYS
                     IQRWDGKSLPAPEDEICG"
BASE COUNT          530 a          555 c          471 g          466 t
ORIGIN      
        1 gtttggcgat cgtaacgccc gtttaccgaa gcgattacat acgcgagccg ctcaagaggc
       61 gcggtctcgg taaaagcctt ccatcgcgca gcatccggca atagagatcc atctcgtaca
      121 acaagcaaag aaagattccg caaggcaaca gtaaccatat ccggaaattg ctcatcgcga
      181 gggaagtgcg gaaaaaccga caggagctcg gctatggttt tttttcgcaa ggaaccatca
      241 ttgcgagggg attcatcaga atgaaggaaa aaactgtaca gtcccgccag agccatatca
      301 tccaccggca acaccgcgtg agtagcctga gaaggaaagg acggtgcggt caacacagaa
      361 agcggatcaa agtccggcag tgcgtcaagg agatgcggat tgatggcata tctacgggaa
      421 gaaaaatcct ttccccgttt cgcgtccccc ccgtcagcct cgcgatagat taccaggcgt
      481 tcttccagat tcaagatacg ctcatagaca tccgggaaac taagagaggg cgcaaggaca
      541 ttaacgatct tttgctgagt tggcacagga agcacctgaa tagcagatag aaccagatag
      601 tccagattat caagggccga caccaagcgc tcccttgttc cgctctttcg caaaaaagct
      661 gaaagctcct cgatgagccg ctgtttatta aaaggggtct tgatctgccc aagatacatt
      721 cgcatcaagt caaaaaaatg ttgatcagaa agacaggtca gggcctcccg ccaggcaagc
      781 acatcctcta cccgcatatc tcatcctccg gtgccggcag gctcttccca tcccaccgct
      841 gaattgaata ttcatagccc tgttcggcaa gaaacttttg gcgattcgcc gcaaaatcct
      901 cttccaccgt tcgggaagta actattgtat aaaaatggga atccttttct ttgggcctga
      961 gtattcgccc caaacgttgg gcttcttcct gacgactacc aaaggttccc gaaatctgaa
     1021 tcgcgacaga agcatccggt aaatcaatgg caaagttcgc aaccttggaa acgaccaagg
     1081 tttttatttt tcccgagcga aattctccat aaatacgatc gcgctctgtg ttgggagttt
     1141 ttccggtaat aagcggagcc cctgttgcac gcgcaatggt ttccagttgt tttatatact
     1201 gcccaataat caatattgaa tcttctctgt gcttctccag caaggcgatt actgcgggaa
     1261 tctttgccgg gttttcgctc gctatacggt acttctcccg ctgtccagct acggcatacg
     1321 aaagctcacg ctcactatcc atatcaacgc gaaattcggt acaactcgcg gtggcaatcc
     1381 agccctgccc ttcaagctcc ttccagggaa catcgtagcg tttaggtcca acaagagaaa
     1441 agacatctcc ctcgcaccca tcctcgcgta ctaaagttgc cgttaatccc acgcgccgta
     1501 cggtttgaat ttcggcggta actctaaaca ccggggccgg cagcaagtgg acctcgtcat
     1561 aaataattaa cccccacgca cgcgagcgaa aaatacggaa gtgtggaaag tcctcttcct
     1621 tgtttggccg ccatgtaagt atttgatacg tcgcgatggt aacgggtcta atttctttaa
     1681 ggtccccgga atactctccg atctcctcgc gggtaagcga cgttttgtcc aaaagttcat
     1741 cacgccattg gtgaactgcc gcaatgttgg tagtaaggat aagggtactt gtttgcagga
     1801 gggtcatcac ttccatgccc accacagtct tgcccgaacc gcaaggcagt acgatagtac
     1861 caaaaccggt gcctggcccc ttatctccca ccagggcacg agccgcgttt ttttgatagt
     1921 cgcgaattac cagcggctta cccgacacac atgctgatcg caatgataaa gaaagtggct
     1981 caccttcctt aaggggcgcc tcatccttaa ccggccaccc ca
//
LOCUS       DAOQXQ010000329         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_64886_length_2021_cov_3.230926, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000329 DAOQXQ010000000
VERSION     DAOQXQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64886_length_2021_cov_3.230926"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1333)
                     /locus_tag="PKH81_05970"
     CDS             complement(119..1333)
                     /locus_tag="PKH81_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNQ97617.1"
                     /translation="MYKIQTLNKIASEGLALFPRDTYEIASEILNPDAVLVRSADMTA
                     MEIPQTVKAIARAGAGVNNIPVAKCTEAGIVVFNTPGANANAVKELVVGALIFSSRPV
                     HKAAQWAKGLAGKGDEIPDLAEKGKSQFVGPEIKGKTLGVIGLGAIGAMVANDAVALG
                     MNVIGYDPFISVDAAWSLSRAVHKAESLDALLTQADYISLHIPQTGDTKGFINADKIR
                     IMKKGARLINFARGGLVNNADVIAALNEGKLAAYVSDFSDEELLATEGVICLPHLGAS
                     TPEAEDNCAFMAVNQLADFLEHGNIVNSVNFPKCRIDGPIPAAGARLCIANRNVPNMI
                     GQITSILAAARLNIASMTNQNRADVAYNLIDVEDRVGDTVMASLKAIDGVIAVRVIDG
                     KANGAVALPEEE"
     gene            complement(1345..>2021)
                     /gene="serC"
                     /locus_tag="PKH81_05975"
     CDS             complement(1345..>2021)
                     /gene="serC"
                     /locus_tag="PKH81_05975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNQ97618.1"
                     /translation="NNTIFGTLMNTFPDTAGIPLVCDASSNILSEPMDVSKFGIIYAG
                     AQKNLGPAGVTVVIIREDLIRGPEHPQGDLPADLATMLRYDTHAAEGSMYNTPPCYSI
                     YVMGMVLEFVKKMGGVPAMAKLNREKAAVLYDYLDSSSFFKATVAKEFRSIMNIPFVT
                     TITDAEKAEAINKKFVAEAEAAGFVNLAGHRLVGGMRASIYNAMPIDGVKALVEFMKA
                     FEKNNA"
BASE COUNT          473 a          505 c          515 g          528 t
ORIGIN      
        1 gattggaaaa ttcaacttat caagtcaaaa aatccaaaat aactcgattt tctcaaaaaa
       61 aagccctctt taccccgttt tttcaggata aagagggctc gatcacacag gaagtagttt
      121 attcctcttc gggaagggca acggctccgt tagcctttcc gtcgattaca cgtacagcta
      181 ttactccatc aatcgcctta aggcttgcca ttacagtgtc gccaacccgg tcttctacat
      241 caatcaggtt gtatgcaaca tcggcacgat tttgattggt catggaagcg atgttgaggc
      301 gagctgccgc aaggatgctt gttatttgac caatcatgtt tggtacgttc cggtttgcga
      361 tgcacagacg ggctccagcg gcgggaatgg ggccatcgat tcggcacttg gggaagttaa
      421 ccgaattaac gatatttcca tgttccagga agtcggccag ctgatttacc gccataaagg
      481 cgcagttgtc ctcggcttcc ggggtcgagg cgccaagatg gggcaggcag ataactccct
      541 cggttgccag aagttcttcg tcggagaaat cacttacata ggcggcaagc ttcccttcgt
      601 tcaatgcggc gattacgtcg gcgttgttta caagaccacc gcgggcaaag ttgataagcc
      661 gtgcgccttt tttcatgatg cgaatcttat ctgcgttgat aaagcctttg gtatctccgg
      721 tttgcggtat gtgcagcgag atataatcgg cttgggttag cagggcatca aggctttcgg
      781 ccttgtgaac cgcacgggaa agcgaccagg cggcatctac cgagataaag ggatcgtagc
      841 caataacgtt catgcctagt gcaactgcat cgttggctac cattgcgcca atagccccca
      901 gtccgataac gccaagggtt tttcccttga tttccggacc aacgaactga cttttccctt
      961 tttctgcaag atcaggaatt tcgtctccct tacctgcaag gcccttggcc cattgagctg
     1021 ccttatggac gggacgactg gaaaaaataa gggcaccaac aaccagctct ttaaccgcgt
     1081 tagcattggc tcccggggta ttaaagacaa cgattcccgc ttcagtgcat tttgcaacgg
     1141 ggatattatt tactccggct cccgctcggg cgattgcctt aacggtttgg ggaatctcca
     1201 tcgcggtcat atcggcagag cgaacgagca ctgcatccgg gttgagtatt tcactggcga
     1261 tctcataggt gtcgcgggga aaaagggcaa ggccctcgct cgcgatcttg ttcagcgttt
     1321 gtatcttgta catgtggtat ctccttaggc gttgtttttt tcaaaggctt tcataaactc
     1381 aaccagtgct tttacaccat cgatgggcat ggcgttatag atagaagcgc gcattccgcc
     1441 cacgagccgg tgtccggcaa gatttacaaa tcctgccgct tcagcctcgg caacaaactt
     1501 cttgttgatc gcttcagcct tttctgcatc agttatcgta gtaacaaagg gaatgttcat
     1561 gatggacctg aattccttgg caacggttgc cttgaagaag ctgcttgaat caaggtagtc
     1621 atagagcacc gcggcttttt cacggttaag ctttgccata gcgggcactc cacccatctt
     1681 tttaacgaac tcaagaacca tgcccataac gtagatagag tagcaggggg gcgtattgta
     1741 catagagcct tctgccgcat gggtgtcgta acgaagcatt gtggcaaggt ccgcgggaag
     1801 atctccctga ggatgttccg ggcccctgat aaggtcttcg cggattatta ccacggttac
     1861 ccctgccggc cccagattct tttgagcccc tgcgtagata atgccgaatt tggaaacatc
     1921 catcggttcc gaaaggatgt ttgacgaggc gtcgcacacg agtggaatac ctgcggtgtc
     1981 cggaaaggtg ttcatcaagg ttccaaagat ggtgttattc g
//
LOCUS       DAOQXQ010000330         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_65015_length_2018_cov_4.075904, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000330 DAOQXQ010000000
VERSION     DAOQXQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65015_length_2018_cov_4.075904"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PKH81_05980"
     CDS             complement(<1..1011)
                     /locus_tag="PKH81_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97619.1"
                     /translation="MAAPTELTVLLRLYTGKQKSPTVIIQDFCEYLQKYARHYLQEAP
                     ELVMYLDDTANTVQRELEELEAKSKVFLSTDLKGKKLAFVPQYYIDRILQRFKEIEEK
                     PEIPFPLGTELPSNYPQAFLKPVYITTDFSSLIESGERTNAYMHQLAFPDETPPMIYP
                     ASLSPEKMLDLAMSKIRFFLRKDESKDYIQKRMMVANPGKELTIKNMLIQFQTRPSES
                     LRAFKHSGDSYLFWNYLCSFIRQDYTKKSEKTPEETSLIQSVFITEYLNNYYKNKVQQ
                     DLQRETALKNLELAFQKPPYHYDMDSITRFTDSRGVPLLGQYQKTDLEAFIQDKTGSA
                     HPN"
     gene            1010..1519
                     /locus_tag="PKH81_05985"
     CDS             1010..1519
                     /locus_tag="PKH81_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97620.1"
                     /translation="MKLVYYTFLLFSMLIFTLPAQGSKIPDGTIVLETAVIMSKGLPG
                     FATNYLDCLQGSYLGPSGAHIMVWISADTLVFSLRDWKESFRAGIKGFESVHDRNLWA
                     CTRVLGVQESLKDKGKWGYFIKLPEGLSDKEGELFIMGFVRRIDGLISGAKYYQDISF
                     PAVITTTGK"
     gene            complement(1530..>2018)
                     /locus_tag="PKH81_05990"
     CDS             complement(1530..>2018)
                     /locus_tag="PKH81_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HNQ97621.1"
                     /translation="VSSKLVGKAGVLKADKKMVVRTMSIIGAAKDAPPSAAIIYFTET
                     DAGKATQVSVTVTNVPDAKADAVKSVGKILAMDIAAFLAPKPVAKKAVAKKPAAKKPA
                     AKKPAAKNAVAKKPAAKKPAAKKPAAKKAVAKKAVAKKPAAKKPATKKTSSSAPTLVI
                     KK"
BASE COUNT          471 a          393 c          535 g          619 t
ORIGIN      
        1 gttgggatgt gccgaaccgg tcttatcttg aataaaggct tccagatcag ttttttgata
       61 ttggcccaac agaggaacgc cccgtgaatc ggtaaaccgg gtaatgctat ccatgtcgta
      121 gtgataaggg ggcttttgaa aggcgagctc aagattttta agggctgttt ctcgctgaag
      181 atcctgttga accttgtttt tatagtagtt attcaggtat tcggtaataa acacggattg
      241 gataagagac gtttcttccg gggttttttc cgattttttt gtgtagtctt gccggataaa
      301 tgagcacagg tagttccaaa acagatatga gtcaccagaa tgcttaaacg cacgcagaga
      361 ttccgacggg cgggtttgaa actgaatgag catgtttttg atggtgagtt cctttcccgg
      421 attggcaacc atcatgcgtt tttgaatata gtcctttgat tcatccttac ggagaaaaaa
      481 acgtatcttt gacatggcta gatcgagcat tttttccggg gaaagggagg cagggtaaat
      541 catgggcggc gtttcatcgg ggaatgcgag ttgatgcata tacgcattgg ttctttcgcc
      601 agattcgatt agcgaagaaa aatcggtggt tatatatacc ggtttaagaa aggcttgcgg
      661 atagtttgat ggaagctcgg ttcccagggg aaagggaatt tcgggtttct cttcaatttc
      721 cttaaaacgc tgaagaatac ggtcaatgta gtattgtgga acaaaggcga gcttcttgcc
      781 cttaagatcc gtagacaaaa agacctttga ctttgcctcc agctcctcaa gttcccgttg
      841 taccgtattg gccgtatcgt caagatacat aacgagttcc ggggcttctt gaagatagtg
      901 acgggcgtac ttttgcagat actcgcaaaa atcttgtata attacggtag gtgatttttg
      961 tttgccggta tatagtctta acaagactgt taattcggtt ggagcggcca tgaagctagt
     1021 ctactataca ttccttcttt tctcaatgct catttttacg ctgcctgcac aaggctccaa
     1081 aataccggat ggtactattg ttttggagac cgcagtgata atgagtaagg gcctgccggg
     1141 gtttgcgaca aattacctcg attgcttaca gggttcctat cttggacctt caggggctca
     1201 catcatggtg tggattagtg cagataccct tgtatttagc ttgcgggatt ggaaagaatc
     1261 ctttcgtgca gggattaagg gctttgaaag tgtacacgac aggaacctgt gggcttgtac
     1321 gcgcgttctt ggcgttcagg aatctttgaa agacaaaggt aagtgggggt attttatcaa
     1381 gttgccggaa ggcttgtcgg acaaagaagg cgaactgttt attatgggct ttgtcaggcg
     1441 aattgacggg cttatatccg gagcgaaata ctatcaggac atcagtttcc ccgcggttat
     1501 tacaaccacg gggaaataac ccttatgatt tatttcttga tgacgagggt gggtgctgaa
     1561 gaagaagttt tttttgtagc gggtttcttg gcggccggct tttttgccac cgcttttttg
     1621 gctactgctt ttttagcagc aggcttcttg gctgcaggtt ttttagcagc gggttttttg
     1681 gctactgcgt ttttagcagc aggtttcttg gctgcaggtt ttttcgctgc gggcttcttg
     1741 gccactgctt tttttgctac gggtttgggt gcaaggaatg ctgcgatgtc catcgcaaga
     1801 atcttcccga cagacttaac tgcatcggct ttcgcatcgg gaacattggt aacggtaacg
     1861 ctgacctgag ttgccttgcc cgcatcggtt tcggtaaagt agatgatagc ggcagaggga
     1921 ggggcatctt tggccgcacc gatgatgctc attgtgcgta caaccatttt tttgtctgct
     1981 ttaagaactc cggctttgcc aacaagtttc gacgaaac
//
LOCUS       DAOQXQ010000331         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_65438_length_2010_cov_3.458312, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000331 DAOQXQ010000000
VERSION     DAOQXQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65438_length_2010_cov_3.458312"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="PKH81_05995"
     CDS             <1..1573
                     /locus_tag="PKH81_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97622.1"
                     /translation="AVLWTFAIILFYYLNGGSMPSGLPFFWVLVIKITEALAGTLINS
                     FIIDALLKEPKQLLNITKLNVRERDHFVELKNIIIPLSGGLIILSHLAFVTWFFLIRE
                     SGSMGPQNPVFSIVLVGSLALVLLFYISVLSKRQDTIQFNLLDSQILALASSNSADLN
                     RKVSILNFDETGHITESLNVYLEVLHQMVTQIRGGCGTLKENESGLSASMFEAEEKLK
                     EINESVQRANDQIAQQIEATDESTRAVTGITGKIQELHAAVSQQTASVDNSSAGIEEM
                     IANITAVSLNVQRVHETCETLLSSAERGKGKINESNTLIGKVVEASALLLDANKMIAA
                     IAAQTNLLAMNAAIEAAHAGAAGAGFAVVADEIRSLAEKSSKQSNIVNSQLKEVRSAI
                     ENAVASANDASTGFDEVLNLINEVTDMERENAEAMKEQKTGSDQVAQTLSEMQQTTNT
                     VNAAAGTLTEESVHLQKTIERLVACSRRARQEMDGIMNDTMAMNATFDEVSELKENNS
                     QIFQHVSEQVGRFIL"
     gene            1602..>2010
                     /locus_tag="PKH81_06000"
     CDS             1602..>2010
                     /locus_tag="PKH81_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97623.1"
                     /translation="MKKDVSSIFLGLIFLAAGVAVGGSMLGFFDFQFSMAGWWTIFII
                     APALISIVQGGFNAGNFIVLAVGVCLLLRAQGYLPAGFSWRLILPGVLLIIGIQLLFG
                     GQSKTKVHFCGCSSTSSSGKPSSDTSTDKTAESD"
BASE COUNT          543 a          520 c          452 g          495 t
ORIGIN      
        1 tgccgtacta tggacctttg ccataatcct tttttattac cttaacggcg gatcaatgcc
       61 ctccggcctg cctttttttt gggtccttgt tatcaaaata accgaggccc ttgcaggcac
      121 cttgatcaac tcctttatca tcgatgctct acttaaggaa cccaagcagc ttctcaatat
      181 aaccaagctc aacgttcggg aacgcgatca ctttgtggaa ctcaagaaca tcatcatccc
      241 cctctctggc ggtcttatca tcctctctca cctcgccttt gttacctggt ttttccttat
      301 ccgggaaagc gggtcgatgg gacctcaaaa cccggtcttt tcaatcgtcc ttgttggctc
      361 tcttgccctc gtccttttgt tttatatcag cgttctttca aaaaggcagg acaccatcca
      421 gtttaatctt ctggacagcc agattctcgc ccttgcctcc agtaactcag ccgatctgaa
      481 ccgcaaggtt tcaatcctga actttgacga aaccggtcac atcaccgaaa gcttgaatgt
      541 atatctggaa gtacttcatc aaatggttac ccagatacgt ggcggatgcg gaacccttaa
      601 agaaaacgaa tcgggacttt cagcgagcat gttcgaggca gaagaaaagc ttaaagagat
      661 aaacgagtcc gttcaacggg caaatgacca aatagcacaa caaattgaag caaccgatga
      721 atccacccgt gcggttaccg gcattaccgg aaaaatccag gaactacacg cggcagtaag
      781 tcagcaaaca gccagtgtag acaattccag cgccggcatc gaggaaatga tagccaatat
      841 taccgcagtg agcttaaacg ttcagcgagt ccatgaaacc tgcgagaccc tgctttcatc
      901 cgcggaacgg ggtaagggaa aaattaacga atccaacaca ctgatcggaa aagttgtgga
      961 agcatctgct cttcttctgg atgcaaacaa aatgatcgcg gctatcgccg cccaaaccaa
     1021 ccttcttgcc atgaacgcgg ctatcgaagc cgcacacgca ggagctgcag gagcggggtt
     1081 cgccgttgtt gcagacgaaa tccggtctct cgcagaaaaa agctcaaagc aatcaaatat
     1141 tgtcaacagt caacttaaag aagtacgctc tgcaatcgaa aacgctgtag cctcggccaa
     1201 cgatgcgtct accggctttg acgaagtgct taacctgatt aacgaagtaa cggacatgga
     1261 acgggaaaac gcagaagcca tgaaggagca gaaaacagga tcagaccaag tggcccaaac
     1321 cctttccgag atgcaacaaa caaccaatac cgttaacgcc gccgcaggaa cactcaccga
     1381 ggaatcggtt catctccaaa aaacaatcga acgtcttgtt gcctgttcac gtcgcgcacg
     1441 gcaagaaatg gacggtatca tgaacgatac catggcaatg aacgcaacct ttgacgaggt
     1501 aagcgagcta aaagagaata attcacaaat attccaacat gtttcagagc aggtgggacg
     1561 ttttatcctc tagaaggtgt tcagttatgc ggaggctttt catgaaaaag gatgtatcca
     1621 gtatctttct cgggcttatc tttctggccg cgggagtcgc ggtcggtggt tcaatgctcg
     1681 gtttttttga ctttcagttt agtatggccg gttggtggac catttttatc atcgcccccg
     1741 cccttatctc tattgttcaa ggcggattta acgcgggaaa cttcatcgtt ctggccgttg
     1801 gcgtatgctt gcttttacgt gcgcagggct atcttcccgc aggctttagc tggcgactca
     1861 tccttcctgg cgttctcctg attattggga ttcagctttt gttcggaggg caaagtaaaa
     1921 ctaaagtaca tttttgcggc tgttcctcca caagctcatc cggcaaaccg tcttcagata
     1981 cctctacaga taaaaccgca gagagtgacc
//
LOCUS       DAOQXQ010000332         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_65599_length_2007_cov_3.911885, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000332 DAOQXQ010000000
VERSION     DAOQXQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65599_length_2007_cov_3.911885"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKH81_06005"
     CDS             complement(<1..798)
                     /locus_tag="PKH81_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNQ97624.1"
                     /translation="MHDKLDRLRVRFAELDTNIQDPLLIKNPAKYRDFMREHAYLSRL
                     MEEYDLYLKAEKDLEETLAMVREADDSDMREMAKDEQHRLEKELEQRESQLKVLLIPP
                     DPLEEKNIIMEIRGGTGGEEAALFAADLFRMYTRFAESKGWKYEILSANETALGGYKE
                     IILSITGKYVYGSLRYESGAHRVQRVPETEASGRIQTSAATVAVLPEAEETEITIRQE
                     DLRIDVMRAGGPGGQCVNTTDSAVRLTHIPTGIVVIQQDEKSQIKNKA"
     gene            complement(1024..1578)
                     /locus_tag="PKH81_06010"
                     /pseudo
     CDS             complement(1024..1578)
                     /locus_tag="PKH81_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614090.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            complement(1568..>2007)
                     /locus_tag="PKH81_06015"
     CDS             complement(1568..>2007)
                     /locus_tag="PKH81_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNQ97625.1"
                     /translation="PNSQEAGARLPYKLRFCDAILQECDRLSAQGIPLILCGDYNIAH
                     KPIDLANPKTNEGNPGYLPEERAWMDRFIGSGYVDTFRAFTSDPGQYSWWSYRFHARE
                     KNIGWRIDYTCVNPGFMDRVKSSTILSDVYGSDHCPVKLSYEN"
BASE COUNT          500 a          436 c          505 g          566 t
ORIGIN      
        1 agccttattc tttatttggc ttttttcgtc ttgctggatt actacaatac cggttggaat
       61 gtgtgtcagg cggactgccg agtctgtagt gttaacacat tgtcccccgg ggcctcccgc
      121 tctcatcaca tcgatgcgaa ggtcttcttg gcggatagta atctcggttt cttctgcttc
      181 aggaagaacg gcaacggttg cggcgcttgt ttgtatgcgc ccagaggctt cggtttccgg
      241 aacacgctgt acccggtgtg ctccagattc atagcgaaga cttccataca catatttacc
      301 ggttatggat agaataattt ccttgtatcc gcccagtgct gtttcgttag ccgataaaat
      361 ttcgtacttc caaccctttg attcggcgaa acgggtgtac atcctgaaaa ggtcagccgc
      421 aaaaagggct gcttcttctc ctccggttcc tccccgaatt tccatgatga tatttttttc
      481 ttcaagggga tcgggcggaa taaggaggac ttttaattga gattccctct gttctaattc
      541 tttttccaga cgatgttgtt cgtcttttgc catctccctc atgtcgctgt cgtcggcttc
      601 ccgaaccatg gcaagggttt cctcaagatc cttttcggct ttaaggtaca gatcgtattc
      661 ttccatcagt cgcgataaat acgcatgttc gcgcatgaaa tcgcggtatt tggcggggtt
      721 ttttatcagg aggggatcct ggatattagt atcaagttcg gcgaaacgaa ctctgagtct
      781 gtcgagttta tcgtgcatac cacactatat caaaatcgag tacaggactt caattggcag
      841 ggttatttag ttttttgttt tccttgtacc gataggaggt ttgcggttta ccggtttttc
      901 ggtaattttt atctttgccg tagatttccc cgtggaagag ggtgctgttg acccataggt
      961 aagggttttt cgttgtgtgg agcggttaac cgttttgttt tgggctatca tcgccgttcg
     1021 cgttcgtacc ggtttaaaaa aaccaaaaag actgccgcat aaaccaccgg caagatgggc
     1081 aaattgggag atatcatctc cgttaaaagc ttgttggatt tctcttccca aatacagaat
     1141 aacaataaga ataaacgtaa ggggaacatc atctttacca tgattggtaa aggatgccag
     1201 gagaatcatc atgaaaacca cgcccgaggc gccaagaagg ggatgcggga aaaagcagac
     1261 atttaacacg ccggttacaa agccggttac aagaatcatc aggaacatgg taaaggagcc
     1321 ataggtttct tcgagcatgg gtcccagaag aaggatgtat gcaagattgc tcatcaggtg
     1381 agtccagtcc gcgtgaccgg cgatatgggt aaagagcctg aggtaattca gggggtccat
     1441 taagttaaag gcaagggcat ttggtacggt aaagagggcc cgggtaaggg atggaaacac
     1501 aaattgagtg gtgagcacaa ttgctgcgca aaagaatgaa aaggtaagag tggtgggggc
     1561 gttgtactta attctcatag cttagtttca ccggacaatg atctgaacca tatacatcag
     1621 aaagaatggt agaagacttc actcggtcca taaaaccggg attgacacag gtataatcaa
     1681 ttctccaccc gatattcttt tccctggcat ggaatcgata actccaccag gagtattgac
     1741 ctggatcgga ggtaaaagct ctaaatgtat ctacatagcc acttccgata aagcggtcca
     1801 tccatgcgcg ctcttccggt aaatatccgg ggttcccctc gtttgttttg gggtttgcaa
     1861 gatcgattgg cttatgggct atgttatagt ccccacacag aatcaggggt atgccctgag
     1921 cgctcaggcg gtcgcattct tgcagtattg catcgcaaaa gcgaagcttg tagggaagtc
     1981 gtgccccggc ttcctgagaa ttgggaa
//
LOCUS       DAOQXQ010000333         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_65758_length_2004_cov_4.817342, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000333 DAOQXQ010000000
VERSION     DAOQXQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65758_length_2004_cov_4.817342"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /gene="thrH"
                     /locus_tag="PKH81_06020"
     CDS             <1..457
                     /gene="thrH"
                     /locus_tag="PKH81_06020"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNQ97626.1"
                     /translation="AEKGLTLMDIQRVIATLDPLPGARAFLDKVRAQTQVIILSDTFT
                     QFALPLMEKLGYPTLFCNDLIIDPNGMVSGYTLRQFDGKKKAVVALQSINMKVMAAGD
                     SYNDLSMITTANDGALFCAPSSIKEAYPQVPAYDDYGALWAHVERFIKS"
     gene            complement(501..>2004)
                     /locus_tag="PKH81_06025"
     CDS             complement(501..>2004)
                     /locus_tag="PKH81_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNQ97627.1"
                     /translation="RDDIDYAPADAKVILGHHFSSIAGAGPITGPIFAAVFGWLPVYL
                     WIVIGSIFIGGVHDAGSLLASVRHDGKSIGEIIKTNISPRAKTIFNWYAWLTITLVVA
                     AFTDICASSFAFDASKPDLISGAQAGTASILFIFLAMLFGFVVYRKNAPIAIASIAGV
                     ILLAFCIWFGFNFPILKMSKLTWQIVMIAYIGFASILPVWLLLQPRDYLCSFLLYAMM
                     AGALVGIIITRPTMQIPAFSGFVASNGQSLFPFLFVTVACGAISGFHSLVSSGTTSKQ
                     IDKEEPDIKLIGYGGMLIEGLLAIVALIAVGYVAGQKGAPAQIFANGVSFFMASFGIP
                     QSVGKVFIILAYSAFALTSLDTATRIGRYIMQEIGVVKTASGEKKTIISNLYVSTLLT
                     VAFSVILLAFGYTKIWPIFGSANQLLAALALLAVTGWIMKQGKKSWETIIPLIFMFSV
                     TLTALTFLIRSNIVGAKHNYVLAGIGILLFVLAILQILEGIRVLFLKKKA"
BASE COUNT          644 a          405 c          453 g          502 t
ORIGIN      
        1 agctgagaaa gggcttacct taatggatat acaaagggtg attgccaccc ttgatccgtt
       61 gccgggagca agagcctttt tggataaggt tagggctcaa acccaggtga taattctttc
      121 cgatactttt actcaatttg cacttccttt aatggaaaag cttggatatc ccaccttgtt
      181 ttgcaatgat ttgattattg atccgaatgg aatggtaagt ggatacacat tgcgccagtt
      241 tgatggaaag aaaaaagcgg tagtagccct tcaaagcata aacatgaagg ttatggctgc
      301 gggggattcc tataatgatc tttccatgat aactacagcc aatgatgggg cccttttttg
      361 tgctccttca tccataaaag aagcgtatcc ccaggttcct gcgtatgatg attacggggc
      421 attatgggct catgttgagc gctttattaa aagctaaaaa aaaaggctgt tcggataatc
      481 cgaacagcct tttttatacg ctatgccttc ttttttagga aaagaactcg aatgccttcc
      541 agaatctgta aaatagcaag tacaaacaac aagattccga taccagctag aacataatta
      601 tgtttggcac caacaatatt tgagcgaatt aaaaaggtaa gagctgtaag cgttaccgag
      661 aacatgaaaa tgagaggaat gatagtttcc caggattttt tcccttgttt catgatccat
      721 ccggttacag ccagaagggc taaagcggcc aatagttgat tggcagaacc aaaaattggc
      781 caaattttgg tataaccgaa agcaagcaat ataaccgaaa aggcgacggt taaaagagtt
      841 gaaacataca ggttagatat aatcgttttc ttttctcctg atgctgtttt aaccacacct
      901 atttcttgca tgatgtagcg accaatacgg gttgctgtat ccaggctggt aagcgcaaag
      961 gccgagtacg caagtatgat aaagactttt cctacggatt gagggatccc aaaacttgcc
     1021 ataaagaatg atactccatt ggcaaaaatt tgtgctgggg cgcctttttg tcctgctaca
     1081 tatccaacag cgatgagtgc gacgatagca agaagccctt caataagcat tccgccgtaa
     1141 cctataagct taatatccgg ttcttcttta tcaatttgtt tggaggttgt cccggaacta
     1201 acaagggagt gaaacccaga aatagcccca caggcaactg ttacaaaaag aaaggggaag
     1261 aggctttggc cattacttgc gacaaaaccg gaaaaagcag gaatttgcat tgtgggtctt
     1321 gtgataataa tcccaactag ggcacctgcc atcatggcat atagcaaaaa agaacaaaga
     1381 taatcgcgag gctgtaaaag aagccaaact ggtagaattg aggcaaatcc gatgtatgca
     1441 atcatgacta tttgccaggt aagcttactc atcttgagaa tggggaaatt aaatccaaac
     1501 cagatacaaa aagcgagaag gataacgcca gctatggatg caatagctat gggagcattc
     1561 ttgcggtaaa caacaaagcc aaaaagcatt gcgaggaaga taaagagaat agaagcagtt
     1621 ccagcttgcg ctccagaaat tagatcaggt tttgaagcgt caaacgcgaa tgaagaagca
     1681 catatgtcgg taaaggcagc gacaaccagc gtaatcgtaa gccatgcata ccagttaaaa
     1741 atggtttttg cacggggaga gatattggtt ttaattattt ctccaatgct ttttccgtca
     1801 tgccgtacag aggcaagtaa tgaacctgcg tcgtgtacgc ccccgataaa aatgcttccg
     1861 ataacaatcc acaaatacac cgggagccaa ccaaaaacag cggcaaaaat gggccctgtt
     1921 atcggacccg ctccggcgat ggatgagaaa tgatgaccaa gaataacctt ggcatcggct
     1981 ggcgcatagt caatgtcgtc acgc
//
LOCUS       DAOQXQ010000334         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_65934_length_2001_cov_3.103289, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000334 DAOQXQ010000000
VERSION     DAOQXQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65934_length_2001_cov_3.103289"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PKH81_06030"
     CDS             complement(<1..1424)
                     /locus_tag="PKH81_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNQ97628.1"
                     /translation="MKTAIAGYPRIGGRRELKFATEAYFAGKIRCEELEETGKQIRRE
                     NWKTLKDAGIDFIPSGDFSFYDLMLDTACMVGAIDSQYRYPGASDLDIRFTMARGRQE
                     HECDLPALPLRKWFTTNYHFIVPHISDDTHFSLTGTGPLEAWKEARDLGIITRPTVIG
                     PFTFLTLASFSGDRTLANSLSSLTAVYVSLLNLLHQEGVSVIQIEEPALALDLNEEAV
                     RHFAQCYREILQSTSRPAIHLQVSFGDVRDAYDAITRLPFEAVGLDFIEGKKNIELLT
                     QKPLLPGTALIAGVISGRNIWKADKGATEHLLEVIKRLCPEVIVGTSCSLLHVPVSLD
                     GETRLDAKIRDKLSFAKEKLDELVFFASREYTAAHGTTSANITRETRQILPDTSETTR
                     TPSRSDRRKIQDKRFCFPQLPATTIGSFPQTHELRMLRAQKRRHEITEEAYDKSIRQM
                     TQECISFQEEIGLEYSGWIIRPQL"
     gene            complement(1503..>2001)
                     /locus_tag="PKH81_06035"
     CDS             complement(1503..>2001)
                     /locus_tag="PKH81_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ97629.1"
                     /translation="VIGILYLNEFNEQVLQKLIREADLRFTPLFTATPHIFVSARHPL
                     AQKHTVHLEDLEPWPCLSFEQGEHNSFHFSEEILSTVSHSKNIRVSDRATLFNLLIGL
                     DGYTISTGVLTSDLNGNAIVSVPLEINDRITVGYITHRSFMASQAACIYIEQLGKYGK
                     ESIID"
BASE COUNT          482 a          448 c          509 g          562 t
ORIGIN      
        1 tgaagctgcg gccgtattat ccaaccgcta tattcaagac cgatttcttc ctgaaaacta
       61 atacactcct gtgtcatctg gcggattgac ttatcgtagg cctcttcggt tatttcatgt
      121 ctacgttttt gtgcgcggag cattcgcaat tcatgtgttt gtggaaacga tccaatcgtg
      181 gttgccggaa gttggggaaa acaaaatcgt ttgtcctgta tctttcgccg atcggagcga
      241 gatggagtcc gtgttgtttc actcgtatcg ggcaatatct gtctggtttc tctggtgatg
      301 ttcgctgatg tcgtaccatg ggccgccgta tattcgcgag aggcaaagaa gaccagctcg
      361 tcaagttttt ccttggcaaa ggaaagcttg tcgcgtatct ttgcatccaa tcgggtttct
      421 ccgtcaaggc ttaccggcac atgaagcaaa ctgcacgaag tgccaacgat aacttcggga
      481 cagagccgct ttatcacttc caggaggtgt tctgttgccc ctttgtcggc tttccagata
      541 ttgcggccac taattacacc cgcgataagg gcagttccgg gaagcagggg tttttgcgta
      601 agcagctcga tgtttttctt tccttctata aaatcaaggc caaccgcctc aaagggaagg
      661 cgggtaatcg catcgtaggc atcgcgaaca tctccaaatg aaacttgaag gtggatagcc
      721 ggacgggagg tactttggag aatttcccgg tagcattgag caaaatgccg taccgcttcc
      781 tcgtttagat caagggcaag ggctggctct tcaatctgaa taacagacac tccttcttga
      841 tgtaacaggt tgagtaagga tacatagaca gcggttagcg atgaaagtga attggcaaga
      901 gtgcggtcgc cgctaaaaga ggcgagtgtt aaaaaggtaa aaggaccaat gactgttggt
      961 cgagtgataa ttcctagatc gcgtgcttct ttccatgctt ccagaggtcc tgttccggta
     1021 agggaaaaat gtgtgtcatc cgagatgtgg ggaacaataa aatgatagtt cgtcgtgaac
     1081 catttgcgta gcggaagggc aggtagatca cattcgtgtt cttgccttcc gcgggccatt
     1141 gtaaagcgga tatccagatc ggatgctccc gggtaacggt attgactgtc tatggcccca
     1201 accatacagg cggtatcgag catcaagtcg tagaacgaga agtcgcccga ggggataaag
     1261 tctatccccg catccttgag cgttttccag ttttcgcgtc tgatttgttt tccggtttcc
     1321 tcaagttctt cgcatcgtat ttttcctgca aagtatgctt ctgtggcaaa cttgagctcc
     1381 cgtcttcccc cgatccgggg atatcccgct atggctgttt tcatatccgg ctccttgtga
     1441 accaactgta ccggatatct ttgttatagt ccaaaacaat ttaactatag cttgttatag
     1501 ttctaatcta ttatagattc ttttccatat tttccaagct gttctatata tatacaggca
     1561 gcttggcttg ccataaacga gcggtgagtt atatacccca cggttattcg atcgttaatt
     1621 tccagcggga cggatacaat cgcgttgcca tttaaatcgc tcgtcaacac tccggtggaa
     1681 atcgtatagc catccagtcc aatcaagagg ttaaaaaggg ttgcgcggtc gctaactcgg
     1741 atgtttttag agtgagagac cgtgctcagt atttcctctg aaaaatggaa ggaattgtgc
     1801 tccccctgtt caaaggatag acagggccac ggttcaaggt cttccaggtg tacggtgtgt
     1861 ttttgtgcca gggggtggcg agcgctaaca aagatatgcg gggtagcggt aaataaagga
     1921 gtaaagcgta gatccgcctc ccggataagt ttttgcagga cttgttcatt aaactcgttc
     1981 aggtacagga tgccgattac g
//
LOCUS       DAOQXQ010000335         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66049_length_1999_cov_2.851852, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000335 DAOQXQ010000000
VERSION     DAOQXQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66049_length_1999_cov_2.851852"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="trpS"
                     /locus_tag="PKH81_06040"
     CDS             complement(<1..512)
                     /gene="trpS"
                     /locus_tag="PKH81_06040"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ97630.1"
                     /translation="METTGKKRILTGDRPTGRLHLGHYVGSIANRVRLQDEYECFFII
                     ADLHTLTTKPEKEHIEELADNVRQMVLDYLACGIDPKKSTIYLQSAVTEVTELNLFFC
                     DMVTVPRLQRIPSIKEMAKNADLSELPFGLLGYPVLQAADILLPRSHLVPVGKDNESH
                     VELTREIAKR"
     gene            589..1716
                     /locus_tag="PKH81_06045"
     CDS             589..1716
                     /locus_tag="PKH81_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ97631.1"
                     /translation="MKYLVLSLISLITVNLIAQDLQLSGGDVKIIQSPEGGYHLYIKK
                     KAGIESVLLTETTKDPSLKADNYAYRALEYNAINGDEKRLLDGVIIPKEKAIWSLIDS
                     TVEQDTPLGEAYHIWIPYIIAYGYSWSRQGEVEVQDGTFFNIRAFSKPYGDYAGTFID
                     NPFKLKLTQKPVPPRQEPPDVRYMSDTVSAFKSLADTSRGQTRYAAGPADIIPLIQAI
                     LENPQNKSLDLVFVIDATESMVDDIATIRELIEPMLDEHLNRYPSWRVSLVLYKDYFE
                     DFLVRVACTFTKSLPAFRKSLNAFRVQGGRDIPEAVYEGLHAALMLPWNEGADRKIIL
                     IGDAPPHPKPRGKISREVVENLAREREVEMNVIIIPHGETY"
     gene            complement(1722..>1999)
                     /locus_tag="PKH81_06050"
     CDS             complement(1722..>1999)
                     /locus_tag="PKH81_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97632.1"
                     /translation="LSQSGQAALDESNFKAAEVYYQLIIDRYGSDPAARTAAEFEIAH
                     MRLKQKKWADARSRLETIIARYEEAGGAGLPPEYLVLAKNDLSKIPQ"
BASE COUNT          516 a          459 c          497 g          527 t
ORIGIN      
        1 aagcgttttg cgatttcccg cgtaagctct acatgacttt cgttatcctt gcccacggga
       61 acaagatggc ttcggggaag gagaatgtcg gcagcttgaa gaaccggata gccaagaaga
      121 ccaaagggaa gctcggacag atcggcattc tttgccattt cttttatcga gggaattctc
      181 tgcagccggg gcaccgtgac catatcgcaa aaaaacagat tgagttcggt tacctcggta
      241 acagcggatt gaagataaat agtcgatttt ttgggatcaa tgccacaggc aagataatcg
      301 agtaccattt gccgtacatt atccgctaat tcctctatat gctctttttc cggtttcgtc
      361 gtcagagtat gaagatcggc gatgataaag aaacattcat actcatcctg caatcgcact
      421 ctgtttgcga ttgaaccgac ataatgacca agatggaggc ggccagtagg gcggtctccg
      481 gtaagtattc tcttttttcc ggtagtttcc atagtgacaa ccagattatc aaaattgacc
      541 ggggtataca agctatactc cagggctaat tccgccgata aaaccagtat gaaatatctt
      601 gttctttcgt tgatatccct tattactgtc aatcttattg cacaggattt acagctttct
      661 ggtggggacg taaaaataat ccagagccct gaagggggct atcatctgta tatcaaaaaa
      721 aaagcgggaa tcgagtcggt cttgttgacg gaaaccacca aagatccctc gcttaaggca
      781 gataactatg cctatcgtgc gcttgaatat aatgcgatta acggtgatga aaaacgcctg
      841 ctcgatggcg tgattattcc caaggaaaag gcgatctgga gtcttatcga ttcaacggta
      901 gaacaggaca ccccgcttgg cgaggcctat catatatgga ttccgtatat aatcgcctat
      961 ggttattcct ggtcgcgtca gggagaggtc gaggttcaag atggaacctt ttttaacatc
     1021 cgcgcttttt ccaaacccta tggcgattac gccgggactt ttatcgacaa tccattcaag
     1081 cttaagctga cgcaaaaacc ggtgccgccc cgccaggaac ctcctgatgt gcggtatatg
     1141 agtgatacgg tttctgcctt caagtctctt gccgatacct ctcgcggaca gacgcggtat
     1201 gccgcaggtc ctgccgatat tataccgctc attcaggcga tactggaaaa cccgcagaac
     1261 aagtcgttgg acctggtctt tgttatcgat gcgacagaaa gcatggtaga cgatatagca
     1321 acaatccgtg aattgatcga gccgatgctc gacgaacatt tgaatcgcta tccatcctgg
     1381 cgggtatccc ttgtgttata caaggattat tttgaagatt ttttagtaag agttgcctgt
     1441 acctttacaa aaagtcttcc cgctttccga aaatcgttga atgctttccg ggtacaggga
     1501 ggcagggata ttccggaagc ggtgtatgaa gggctacatg ccgcgctgat gcttccctgg
     1561 aatgagggtg cagaccgaaa gataatcctg atcggagacg cgcctcccca tccaaaaccg
     1621 aggggaaaga tatcccggga agtagtcgag aaccttgccc gggagcgaga agttgaaatg
     1681 aatgtgataa ttattcccca tggggagacg tactaagtct cttattgcgg gatcttggaa
     1741 aggtcgtttt tagctaatac cagatattcg ggggggagac cggctcctcc tgcttcttca
     1801 tagcgggcga taatagtctc gagccgactt cgtgcgtctg cccatttctt ttgttttaga
     1861 cgcatatggg caatttcgaa ctcagctgcg gtgcgtgcgg caggatctga tccatagcga
     1921 tcaataatga gttggtagta cacctcggca gccttgaagt tactctcatc cagggcggct
     1981 tgaccgctct gactgagtt
//
LOCUS       DAOQXQ010000336         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66073_length_1999_cov_1.785494, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000336 DAOQXQ010000000
VERSION     DAOQXQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66073_length_1999_cov_1.785494"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKH81_06055"
     CDS             complement(<1..1034)
                     /locus_tag="PKH81_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HNQ97633.1"
                     /translation="MSKEKIKPSEVIEEISDSMTPTSNLEDIQEFMKQYDLESVYREH
                     KCWKRYITIVVSLAFCAFQLYATLSGAVTAQILRSTHLAFVMLLCFLMYPPTRKMRLD
                     TLPMYDVGLALVGFSAWMYIALNFETLARRAGAYLPLDIFVGVVGIVLLFEACRRVAG
                     LPMMIIAGSFILYAFLGPWLPAFFNHRGYSFERVVSHLFFNTEGIMGTPIGACASFIF
                     LFIMFGSFLEKTGIGQFFIDICNAIAGGAAGGPAKVAVLTSALEGTVSGSSVSNTVGS
                     GSFTIPMMKRLGYKPEFAAAVEAAASTGGQIMPPIMGAAAFLMAESLGLSYLTIAKAA
                     IIPALLYFSG"
     gene            complement(1055..1630)
                     /locus_tag="PKH81_06060"
     CDS             complement(1055..1630)
                     /locus_tag="PKH81_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HNQ97634.1"
                     /translation="MPLLANRDGIAMERFRLLLVLPSCATAFLVLLLCCIPLFPALVV
                     SSFEHPRLRIYLDRPYLVPAMNQHLFFAGKPPSFIVSYTHSVNKGRVYDYCRIGDDGL
                     VHVDKSRFVSYGAGMSEPEEGMVFTTGEGYLEISGINLVVPQVVLRVSLTGEHAIEAN
                     GRIWRLADYFPRQSAVVIEKRAVPLLYCIYP"
     gene            complement(1632..>1999)
                     /locus_tag="PKH81_06065"
     CDS             complement(1632..>1999)
                     /locus_tag="PKH81_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNQ97635.1"
                     /translation="ALQELAISAGLVLIPVDGTQAAAIRAEHAFYTEMVIPAGTYKGT
                     EVDAKTLAIKATLAVSAKLDEKTVYAMTKALFENQAELAQSHAKGKDLNAAYSVSGVS
                     VPFHPGAAKYFAEIGLAVK"
BASE COUNT          604 a          504 c          474 g          417 t
ORIGIN      
        1 atgccggaaa aatataaaag ggcaggaata atcgccgctt tagctatcgt taaataggaa
       61 aggccaaggc tctctgccat caaaaaagcg gcggctccca tgatgggagg cattatttga
      121 ccgccagtag aggccgcagc ttctacagcg gcggcgaact caggcttata gccaagacgc
      181 ttcatcatcg ggatagtaaa acttccggaa ccgactgtat tggaaaccga actaccagag
      241 actgttccct caagggcgct cgttagaacc gcgaccttgg caggcccacc ggcggcgccg
      301 ccggcgatgg cgttgcagat gtcgataaag aattggccaa taccggtctt ttccaagaaa
      361 gagccaaaca tgataaacaa gaagataaag gaggcacagg cgccaattgg cgttcccatg
      421 atcccttcgg tattaaaaaa taagtgagat accacacgct caaaggaata ccctcgatga
      481 ttaaaaaagg cagggagcca ggggccaaga aaggcataga ggataaaaga gccagcgata
      541 atcatcatgg gaagacccgc aacacgccgg cacgcttcga acaagaggac aataccgaca
      601 accccaacaa aaatatcaag cggaagatac gcacccgcac ggcgggcaag ggtttcaaag
      661 ttcaaagcta tatacatcca ggccgaaaac ccgacaagag ccaatccaac atcgtacatc
      721 ggcaaggtat caaggcgcat ctttcgggtg ggaggataca ttaaaaagca aagaagcatc
      781 acaaaagcaa gatgggtaga gcgcaagatt tgcgcggtta ccgccccgga cagcgttgca
      841 tacaactgaa aggcgcaaaa cgcaagagaa acaacaatag taatatatcg cttccagcac
      901 ttgtgctcac gatatactga ctcaaggtca tattgtttca tgaactcctg aatatcttca
      961 agattcgatg ttggtgtcat cgaatcacta atttcctcga taacctcgga tggtttaatt
     1021 ttctccttgg acacagtagc aactccttta ggtatcacgg atagatacaa tacaacaggg
     1081 gcacagccct tttttctatg acaaccgcgg attgacgggg aaaataatcc gcaagacgcc
     1141 atatgcgccc gttagcctct atggcgtgtt cgccagtaag cgagacccta agcaccacct
     1201 gcggaacaac aagatttatt ccgcttattt ccaaataccc ctctcccgtg gtaaagacca
     1261 tcccttcttc gggttcgctc ataccagctc catacgatac aaagcgactt ttatccacat
     1321 ggaccaaacc gtcgtcgcct attctacaat agtcatacac tcgaccctta tttacagaat
     1381 gggtatagga tacaataaat gaggggggct ttccagcgaa aaataaatgc tgattcatcg
     1441 caggtaccag atagggacga tcaagatata tcctgagtcg gggatgttca aacgatgata
     1501 caaccagagc gggaaagagc gggatacaac acaataacaa aaccaaaaaa gctgttgcac
     1561 aagacggaag gacgaggaga agccgaaacc gctccatcgc aatcccgtcc cggtttgcca
     1621 acaatggcat cttacttaac cgcgagtcca atttccgcaa agtattttgc tgcacccgga
     1681 tggaagggaa cagagacccc ggataccgaa taggcagcgt taaggtcttt acccttggca
     1741 tgagactggg ctagttctgc ctgattttca aacaaggcct ttgtcatggc atataccgtt
     1801 ttttcgtcga gtttggcgct taccgcgagt gtcgccttga tagccagggt ttttgcgtca
     1861 acttcggttc ccttatacgt tccggctgga ataaccatct cggtgtaaaa cgcgtgttct
     1921 gcgcgaatgg ccgcagcctg ggttccgtcc actggaataa gaaccaaacc agcgctaatc
     1981 gcgagttcct gaagcgcag
//
LOCUS       DAOQXQ010000337         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66261_length_1995_cov_2.335567, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000337 DAOQXQ010000000
VERSION     DAOQXQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66261_length_1995_cov_2.335567"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKH81_06070"
     CDS             <1..370
                     /locus_tag="PKH81_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HNQ97636.1"
                     /translation="LTSPKSGCIKVYRARLGQPVDEEAFSLYQKRFTQGIQFHDGYTC
                     KSAVVERGEGVDEMIVHISEGKYHQVKKMFKALGNEVVYLKRLSMGSLILDESLAPGS
                     IRELTGEEVAALREGYGPAD"
     gene            complement(396..1178)
                     /locus_tag="PKH81_06075"
     CDS             complement(396..1178)
                     /locus_tag="PKH81_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNQ97637.1"
                     /translation="MKRLTLFLCALLSTTILSALPIPPLNEPVTDLAGLLRPDARQRI
                     NSYLHEVHNQTGVHIAVLTIPSLEGETIEEYSMSVAEKWQLGQKDADNGVLLLIALND
                     RAMRIEVGYGLEGTLTDTKSGLIIREVMAPRFREGNWELGIYDAVRTIAGITTGNEEW
                     ISKAVSNPETATNSSGNGPVLIAFILFYLLFSITAGRRKGGLGGGFLWPFVIMSLLNQ
                     HQGHSSGRGGFSGWGGSGGGFSGGGFSGGGGSFGGGGASGGW"
     gene            complement(1175..1873)
                     /locus_tag="PKH81_06080"
     CDS             complement(1175..1873)
                     /locus_tag="PKH81_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97638.1"
                     /translation="MNNERLMRNISLQPADLAELEKAIQMTEQSSSGEIVIAIAEESS
                     DYSFRELFAAVLFGAAVFTLLLLCNSYVTQWMKYHWWHVEPSLVTAVTGAVSFLAMAV
                     FFIFANVPAIDSLIVPKKEKEQAVYRRALRHFVESGVYATKERTGILIYISCMERQVR
                     ILADTGIRTKIDESYWKDRALEVAHGIKTGTTKKALLSTIQAMSTILSQHFPPSPQNP
                     DELENAITFIRRGQ"
     gene            complement(1870..1986)
                     /locus_tag="PKH81_06085"
     CDS             complement(1870..1986)
                     /locus_tag="PKH81_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ97639.1"
                     /translation="MFIKQFPRSFIANLSGFREKPYFKADEAAQSAPQVSFE"
BASE COUNT          486 a          482 c          509 g          518 t
ORIGIN      
        1 attaacctcg ccaaaatccg gctgtatcaa ggtgtatcgg gcgcggttgg ggcagccggt
       61 ggacgaagaa gcgttctcgc tctatcaaaa acgctttaca caggggattc agtttcacga
      121 tgggtacacc tgtaaaagcg ccgttgtgga gaggggcgaa ggtgtcgacg agatgatagt
      181 ccatatcagc gaaggtaaat atcaccaggt aaaaaagatg ttcaaggcac tgggtaatga
      241 ggttgtctat cttaagcgcc tgtccatggg atcattgatt ctggatgagt ctctggcgcc
      301 gggaagtatt cgggaactaa ccggggaaga ggttgccgca ttgcgggaag ggtatgggcc
      361 agctgattag gatgagcggt accgctttcc cctggctacc agcctcccga agcgccgcca
      421 ccgccaaagc ttcctccgcc tccggaaaaa ccaccgcctg agaatccgcc ccctgagcct
      481 ccccatccgg aaaatccacc tctaccgctt gagtgtccct gatgttgatt aagcaatgac
      541 atgatgacga agggccagag aaatcctcct ccaagacctc cctttcgtct gccggcggtg
      601 atagaaaaaa gaagataaaa gagtataaaa gcaatcagga caggaccgtt ccccgagctg
      661 ttggtagcag tttctggatt gctcactgcc ttggaaatcc attcctcgtt tccggtggta
      721 atgccggcga tagtgcgaac ggcatcgtag atccccagtt cccagttccc ttctctaaag
      781 cggggagcca tgacctctct gataatcaaa ccactttttg tatcggttaa tgtaccttca
      841 aggccatatc ctacctctat gcgcatggcc cgatcattga gggcaataag gagaagcaca
      901 ccgttatcag cgtctttctg cccaagttgc cacttttccg ctacgctcat cgagtattct
      961 tcgattgttt caccttcaag cgatggtatg gttaacaccg caatgtgaac gccggtttga
     1021 ttgtgtactt catgtagata ggaattgatg cgctgacgag cgtctggacg taaaagtccg
     1081 gcaagatctg ttaccggctc gttgagcgga ggaattggga gcgcagaaag tatagtggtg
     1141 gagagtaatg cacaaagaaa cagggtcagt cgcttcattg tcccctcctt ataaaggtta
     1201 ttgcattttc aagttcatcg gggttttgag gggaaggagg gaagtgttgg gatagaatag
     1261 tcgacatagc ctgtattgta gaaagaaggg ctttttttgt cgtgccagtt ttaattccgt
     1321 gcgctacttc caatgctcga tccttccagt aggactcgtc tatctttgtc ctgattccag
     1381 tatcagcgag tatcctcacc tgtctttcca tacaggagat gtatatcaat atgccggttc
     1441 tttctttggt tgcgtacacc ccgctttcta caaagtgacg aagtgccctt cgatacactg
     1501 cctgctcttt ctcttttttg ggaacaatca ggctgtctat tgccggtacg ttcgcgaata
     1561 taaaaaacac agccattgca aggaacgaga ctgccccggt taccgcggta accagggagg
     1621 gctctacatg ccaccagtgg tatttcatcc attgggtgac atagctgttg cacaaaagta
     1681 gcagggtaaa aaccgccgct ccaaagagaa ctgcggcgaa cagttcccta aaggaataat
     1741 cagagctttc ctctgctatc gcgataacaa tctcgccgct actggattgt tctgtcatct
     1801 gtatcgcttt ttcaagctct gcaagatcgg caggttgtag cgagatgttt cgcattagtc
     1861 gttcattgtt cattcaaacg atacctgtgg ggcgctctgt gccgcttcgt cagccttaaa
     1921 gtagggtttt tccctgaacc cgctcaggtt cgcaataaag gagcggggaa attgcttaat
     1981 aaagatgttg taggt
//
LOCUS       DAOQXQ010000338         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66356_length_1993_cov_3.486584, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000338 DAOQXQ010000000
VERSION     DAOQXQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66356_length_1993_cov_3.486584"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="PKH81_06090"
     CDS             complement(<1..1593)
                     /locus_tag="PKH81_06090"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ97640.1"
                     /translation="MKKLIFVTGGVCSSLGKGVAASSMGALMEARGLNVRMIKVDPYI
                     NVDAGTMSPYQHGEVYVTDDGAETDLDLGNYARFTHSPLSRANSITTGQVYRDVITKE
                     REGRYLGHTVQVIPHITDNIKERIKAIFLDPDADITIVEVGGTVGDIESIPFLEAARQ
                     MIHELGKKDCITVHLTLIPQVSGGELKTKPTQHSVKELQEMGIQPDILLCRSSEPIDD
                     GMRKKISSFTNVSFDAVISAYDVAPSVYELPLVFHAQKLDQIILEKLGIESRHSVLTA
                     WKKTVDCLKNPKGRLRIGVVGKYMDLGDAYKSVYESLVHGGIANDVAIDIIKLDSARF
                     EKNGCDEAGDPLAGIDGVLIPGGFGERGIEGMIEAARLAREKKIPLFGICLGMQIMVI
                     EWARTILGWKDAHSGEFDSNTSHPVVSLLEEQRGVTSYGGTMRLGKSLSVLKEGTLIK
                     AAYGTERIFERHRHRYEVSNQYRSDLEGSGLLLSGLTEGGELVESVQWPDHPWGVGVQ
                     FHPEFISSPVSPHPLFASFIKAA"
     gene            1733..>1993
                     /locus_tag="PKH81_06095"
     CDS             1733..>1993
                     /locus_tag="PKH81_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ97641.1"
                     /translation="MKTRSAPRLTLRHLSWPMFIEQMTGGLCAFVDTLFLSMISDTAA
                     GSVGMLGPIMMVGYFVLPQFTSAGTSVASQYLGAEREDKAIPS"
BASE COUNT          471 a          497 c          495 g          530 t
ORIGIN      
        1 tgcagccttg ataaaggaag cgaacagggg atggggggat acgggacttg agataaactc
       61 gggatgaaac tgtactccga ctccccaggg atggtctggc cattgtacgg attcaaccaa
      121 ttctccccct tcggttaagc ccgaaagaag tagtcctgat ccttccaaat cggaacgata
      181 ctggttagaa acctcatagc ggtgacgatg gcgttcaaag attcgctcgg taccataggc
      241 tgctttgatc aatgtacctt ccttgagtac cgaaaggctt tttcccaaac gcatagtgcc
      301 tccgtacgag gtaacgcctc gctgctcttc caaaagggag acaaccggat gggatgtgtt
      361 actgtcgaat tcgccggaat gtgcatcctt ccaaccgagt atcgtgcgcg cccattcaat
      421 taccataatt tgcatgccaa gacagatgcc gaacaggggg atttttttct ctcgtgcaag
      481 acgagctgct tcgatcattc cttctatacc tcgctcccca aagcctccgg ggataagcac
      541 tccgtcaata ccggcgaggg gatctcctgc ctcatcacag ccgttttttt caaagcgcgc
      601 ggaatctagt ttgataatgt ctatggcaac atcattggcg ataccgccgt gaacgaggga
      661 ttcatagacc gacttgtagg catcccccaa gtccatgtat ttgccaacca ctccaatgcg
      721 aagtcgcccc tttggatttt tcaggcaatc taccgttttt ttccatgcgg taaggactga
      781 gtggcggctt tctattccca atttttccag gatgatctgg tccagttttt gcgcgtgaaa
      841 aaccaatgga agctcgtata ccgaaggggc aacgtcgtag gcagaaatta ccgcgtcaaa
      901 ggatacgttg gtaaaactcg atatcttctt ccgcatacca tcgtcaattg gctcggatga
      961 cctacacagg aggatgtccg gttgtattcc catttcttgc agttctttaa cggagtgttg
     1021 ggtaggtttg gttttaagtt ccccgcccga aacttgcgga ataagggtaa gatgaacggt
     1081 aatgcagtct tttttgccaa gttcgtggat catttggcgc gcggcttcca aaaaaggaat
     1141 agattcaatg tcgccaacgg ttccgccaac ttcaacgatg gtgatatccg cgtcgggatc
     1201 aaggaagatc gcctttatcc gctctttgat gttatcggtt atatggggaa taacttggac
     1261 ggtatgaccg aggtaacggc cttccctttc tttagtgatg acatcccggt atacctggcc
     1321 ggtagtaatg gagtttgctc tggatagtgg cgagtgggta aatcgggcat agttgcccaa
     1381 atcaaggtct gtctcggcgc catcgtcggt aacatagacc tctccatgct gataggggct
     1441 catggttcct gcatccacat tgatgtaggg gtctaccttg atcattctga cgttcaagcc
     1501 ccgcgcttcc atcagggctc ccatggaaga tgcggcaact ccctttccca gggaagaaca
     1561 tacgccgccg gtgacgaata taagtttctt catgggatta cattatccaa acaactgcct
     1621 ggtaaagcaa gtgtttgccc cgactgccac tggataaaat atgagcatgt tctgattgaa
     1681 atgcgggcat tgctagccaa gattcccggg gatttgatac actgccatcc atatgaaaac
     1741 cagatccgct ccacgattaa ccttacggca tctttcctgg ccaatgttca ttgaacagat
     1801 gaccgggggc ttatgtgcct ttgttgacac cctttttttg agcatgatct ctgacacggc
     1861 tgccggctca gttggcatgc ttgggccaat aatgatggtg ggttattttg tgctcccaca
     1921 atttacctcg gccggaacca gcgttgcttc ccaatatttg ggagccgagc gggaagataa
     1981 ggcgatccct tcc
//
LOCUS       DAOQXQ010000339         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66418_length_1992_cov_3.013939, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000339 DAOQXQ010000000
VERSION     DAOQXQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66418_length_1992_cov_3.013939"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1565
                     /gene="radA"
                     /locus_tag="PKH81_06100"
     CDS             144..1565
                     /gene="radA"
                     /locus_tag="PKH81_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNQ97642.1"
                     /translation="MSAKKKTGSTAYRCSSCGYTQPRWLGRCPDCGEWNTFEETQMHD
                     SPAPRVPGASGASASRPVPLSAVEARDGARLETGIEELDRVLGGAAMKRSAILVGGEP
                     GIGKSTLLLQAAAGAQTKGRVLYVSGEESAAQIRSRADRLSLDTRSVEVLCTSSLEDI
                     HRALTEVNPVFVIIDSIQTVYAPEAGSVPGTVNQLKLCSHDLVSWVKERDSVLFLAAH
                     VTKDGTIAGPKALEHLVDTVLSFERNDDDIRFLRAVKNRFGSVDELGLFQMTGKGLFP
                     VEDPSALFLVRRNTGLPSGVAVTAVFEGSRVFLVEIQALTVPAKGSLTRVFSDRIDSS
                     RVSRVAAVLEKRLQIRLSDQDIYVNVAGGVRLSESAIDLALAGALYSARTDIPLPEGY
                     ALIGELSLAGEVRPVNRLKARVKAALGLGFTHVIAPSAEEGALGAQDIRKMISLLFNE
                     APHKGGHRDKVADRSSQTGKEKD"
     gene            complement(1482..>1992)
                     /locus_tag="PKH81_06105"
     CDS             complement(1482..>1992)
                     /locus_tag="PKH81_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HNQ97643.1"
                     /translation="LIRKIAAGSASADTSDIETVIKPGFFSRAAGWLLPALIVALIAA
                     GFFTGGAMSSMEMLGRWLLWNGSLAALGTLVALGHPLAILTAFVGAPVATINPFIGVG
                     LFSGLAQAWIKKPRVSDMESLAQDVTHIKGFYRNRISHVLLIFFLSSLGGAIGNFIAV
                     PALVGSLVK"
BASE COUNT          436 a          477 c          598 g          481 t
ORIGIN      
        1 actggaggca agcagcagta aaccaagaat caatttttta aaaggacagc cctggaagtg
       61 catagcagaa tagtaacttg tttggtaaaa aaaatccgcc ttcttgcggg cgatggcgcg
      121 tttttttatg ataagatagc cgtatgagcg caaaaaagaa gacgggatcg acggcctatc
      181 gctgttcatc ctgtggatat acacaaccca ggtggcttgg ccgctgtccg gattgcgggg
      241 agtggaatac ctttgaagaa acccagatgc acgacagtcc tgctccccgc gttccggggg
      301 ccagcggggc ctcggcttct cgtccggtac cgctttctgc tgtagaggcc cgcgatgggg
      361 ctcgactgga aaccggaata gaggagttgg accgggtcct gggtggagct gccatgaagc
      421 gctctgcgat tctggtggga ggggagccgg gtatcggtaa gtccaccctt ttgcttcagg
      481 cggcggccgg agcccaaacc aaaggccgtg ttttatatgt ttccggagag gagtccgccg
      541 ctcaaattcg atcccgggca gatcggcttt ctctggacac ccgatcggtg gaggtactgt
      601 gtacgtcttc cctggaggat atacatcgag ccctgaccga ggttaatccg gtatttgtga
      661 ttatcgactc aattcaaacg gtgtatgctc ccgaagcggg ctctgtgccg ggaacggtca
      721 atcagttaaa actctgttcg cacgacctgg tgtcctgggt aaaggagcgg gattccgttc
      781 tgtttcttgc ggcccatgtt accaaggatg ggacaatagc aggccccaag gctctggagc
      841 atttggtgga taccgtcctt tcttttgagc gaaacgatga tgatatccgt tttttgcgtg
      901 cggttaaaaa tcgttttggc tctgtagacg agcttggtct ttttcagatg acggggaagg
      961 gtttgtttcc tgtggaagat ccatctgccc tttttttggt ccgcaggaat actggtttac
     1021 cttcgggggt agcggtaacg gcggtattcg aagggagtcg tgtttttttg gtggagatac
     1081 aggcgcttac ggtgccggca aagggctcgc ttacccgcgt gttttcggat cgtatagaca
     1141 gttcccgggt aagccgggtg gcggctgtgt tagaaaaaag attgcagatt cgactgtcgg
     1201 atcaagatat ctatgtgaac gttgcaggcg gtgttcgcct gagcgagagc gctattgacc
     1261 tggctcttgc cggagctctc tactccgcgc gaactgatat tcccttgccc gaaggctatg
     1321 cccttattgg tgaattgagt ttggcgggag aggttcgccc ggttaaccgg cttaaggcgc
     1381 gcgtcaaggc cgctctgggc cttgggttta cccacgttat tgccccctcc gcggaggaag
     1441 gggccttggg cgctcaggat attcggaaga tgatcagctt gttatttaac gaggctcccc
     1501 acaagggcgg gcaccgcgat aaagttgccg atcgctcctc ccaaactgga aaggaaaaag
     1561 attaacagga catgggagat gcggtttcgg taaaagcctt ttatgtgtgt tacatcctgc
     1621 gcaaggcttt ccatgtccga gactctgggt tttttaatcc atgcttgagc cagaccggaa
     1681 aaaaggccaa caccgataaa ggggttaatt gtggcaacgg gggccccaac aaaggcggtg
     1741 agtatggcaa ggggatgacc cagggctacc agggttccca atgcggccag agagccattc
     1801 cacaaaagcc atctaccaag catttccatt gaagacatcg ctccgccggt aaaaaatccg
     1861 gcggcgataa gagctactat aagagcgggg aggagccatc ccgcggcgcg agaaaagaat
     1921 cctggtttta tcaccgtttc tatatcgctg gtgtcggcgc tcgcgcttcc cgctgcgatt
     1981 ttgcggataa gg
//
LOCUS       DAOQXQ010000340         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66463_length_1991_cov_3.659091, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000340 DAOQXQ010000000
VERSION     DAOQXQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66463_length_1991_cov_3.659091"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKH81_06110"
     CDS             <1..393
                     /locus_tag="PKH81_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97644.1"
                     /translation="VMVAILSVRFTVIKAINLCASPLLAVLVTLSILGYAGIPLNLFS
                     IMALVLVTGIGVDYSIFLGEGGQSRELTFFGVMLCMFTTLFSFGALAFSSFAPVQTFG
                     ITLVCGILLAFIISPFVVCETNEFNGGK"
     gene            397..984
                     /locus_tag="PKH81_06115"
     CDS             397..984
                     /locus_tag="PKH81_06115"
                     /inference="COORDINATES: protein motif:HMM:NF023089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HNQ97645.1"
                     /translation="MRIYKRIPLCFFSIGTLVVFFACTGSPSTRKADNPVFLTNSVMY
                     TFLDPSCIATNIDEVQRIYGAYGEREFSFLMYTIADDNGITLLVLNEMGSEVARITYA
                     DGIVGTSDLVRRLGFPAEYIVADFQLAFYRLDCLVSALSSKGISINEDFSPRVRRFSE
                     GNKEILRIDYYEKSLEFINSLRGYRYTIEWGATDE"
     gene            977..>1991
                     /locus_tag="PKH81_06120"
     CDS             977..>1991
                     /locus_tag="PKH81_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNQ97646.1"
                     /translation="MSKELVYLSDFGVVSSLGKGKAVLWDAVSKGTPSGIKSVTRMGR
                     EWSFALCQNDKSAEDNRVAALLQVALEEILPSIHKAIHRWGSSRVGVCVGSCDNGSEK
                     SFASLGQKRKTGSYPEEYSLQDQQANVPAVFLRRYLGLSGPAYAYSTACASSASALAG
                     ARNLIRSGMCDAVIAGGVDIVSDAVALGFAALEAVSAKPCNPFSCNRSGINLGEAAAL
                     FLLTREDLFEQSPILSGIGESSDAHHMTAPDPSGSGAALAMDRALVDAQLENNAIDYI
                     NLHGTGTQLNDAMESRALHKVFEVIPPVSSTKGITAHTLGAAGALELGICWLALSADI
                     TQRL"
BASE COUNT          485 a          376 c          488 g          642 t
ORIGIN      
        1 gtaatggtgg cgattttatc tgtacgtttt actgtgatta aggcaataaa tctgtgtgcg
       61 tcgcctttat tagctgtact ggtcacgctt tcgatcctag ggtatgcagg gattcctctt
      121 aatctttttt cgataatggc cctggtgtta gtaacaggca taggcgtgga ttattcaatc
      181 ttccttggag agggcggtca atctcgggaa ctaacttttt ttggtgtaat gctttgtatg
      241 ttcacgactc ttttttcttt tggggccttg gcttttagtt cttttgcgcc agttcagacc
      301 tttggaataa cgttggtttg cggaattctt ttagccttca ttatttcacc ttttgtggta
      361 tgtgaaacaa acgagttcaa tggaggcaag taattgatgc gaatatataa acgaatacct
      421 ctgtgttttt tttccatcgg cactcttgtt gttttttttg catgtactgg ttcgccttct
      481 acaagaaaag ccgataatcc tgtttttctg actaattctg tgatgtatac tttcctggac
      541 ccttcatgca tagcaacaaa catagacgaa gtacagcgta tatatggagc gtacggagag
      601 cgtgagtttt catttttaat gtataccatc gctgatgata acgggattac gcttcttgtc
      661 cttaatgaaa tgggttcgga ggtagcgcgg attacatatg ccgatggtat agttggtact
      721 tctgacttgg taaggcgtct tggttttccg gcagaataca ttgtagcaga ttttcagctt
      781 gctttttatc gtttggattg tttagtttca gcgttgtctt ctaagggaat ttctataaac
      841 gaagactttt ctccgcgagt ccgtcgtttc tcggaaggta ataaagaaat cctgcggatt
      901 gattattacg agaagtccct tgagtttatc aatagtctaa gaggatatag atatacgata
      961 gaatggggag ctacagatga gtaaagaact tgtctacctg agcgattttg gtgttgtctc
     1021 atctttggga aaaggaaaag ctgtattgtg ggacgctgtt tcaaaaggaa cgccaagtgg
     1081 aattaagtct gttacccgaa tgggcagaga atggtctttt gccttgtgcc aaaacgataa
     1141 atcagctgag gataacaggg tggctgcatt gttgcaagtt gctttagaag agattcttcc
     1201 ctctatacat aaagctatac atcgttgggg ttcaagtcgg gttggtgtgt gcgtaggttc
     1261 ctgtgacaat ggttccgaga aatcttttgc atcgcttggt cagaaaagaa aaacggggag
     1321 ttatccagaa gagtattctt tgcaagacca acaagctaat gtacccgccg tgtttctacg
     1381 tagatatctt ggtctttccg gtcctgctta tgcctactcc actgcatgcg cttcaagtgc
     1441 aagtgcctta gcaggcgcaa gaaatttaat tcgatcaggg atgtgtgatg cggtaattgc
     1501 tggtggcgtt gatattgtgt ctgatgccgt agcccttggg tttgcagccc tggaagcggt
     1561 atcagctaaa ccatgcaatc cttttagctg caatcgatca ggaataaatc ttggcgaagc
     1621 agctgcgctc tttttgctta cacgggaaga tctttttgag caatccccga tactttcggg
     1681 aattggagaa agttctgatg cgcaccacat gacggcccca gatcctagtg gaagcggggc
     1741 ggctcttgca atggatcgcg cccttgttga tgcacagcta gaaaacaatg ctatcgatta
     1801 tattaatctt catggtacag ggacccaatt aaatgatgct atggaaagca gggctttaca
     1861 taaagttttt gaagtgattc caccagtaag ttccacaaaa ggaataactg cacatactct
     1921 cggtgccgcg ggcgctctgg aacttggaat ttgttggtta gctctttcgg ctgacattac
     1981 tcagcggttg t
//
LOCUS       DAOQXQ010000341         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66475_length_1991_cov_3.174070, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000341 DAOQXQ010000000
VERSION     DAOQXQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66475_length_1991_cov_3.174070"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..177
                     /locus_tag="PKH81_06125"
     CDS             1..177
                     /locus_tag="PKH81_06125"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNQ97647.1"
                     /translation="MAGNAASAILSASIDISPPVVVRGENHQIAWPKTMPVVAIPFTT
                     SCGPFEVDVCFKHK"
     gene            202..>1991
                     /locus_tag="PKH81_06130"
     CDS             202..>1991
                     /locus_tag="PKH81_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97648.1"
                     /translation="MKVLPKSLRVRFVLLFSLFIAVLCVVSGWLSAKAMVDTATSLFS
                     EKGAPLSREVASRLDPQRYQALVSSLDPLDPYYSDTHLWMRERKEESGCTFLYTMSRT
                     EDGVYRYIIDGSSAMDDEENFSPLGAEEDAESFGPAFARAMDQGLPSHSRLVFQEGWG
                     WLISVYYPIKNSAGAVIGVVGCDFAAEALRHDISRYVLRQVLVAGICLVAGIVLLLFL
                     TNLVFAPIKLMSAPMSSIADGSGDLTVTIPVRAQNEISVLANGFNRFVSSLRDMVVST
                     RGAVDNLTVAGQTLAENSRQASMSVEAITGEIEAIRTLAGRQDQMTIETFESISALER
                     RLAELDNQVSSQTDKLNSSFAAIEEMTANITAINTTIERMVSQYNELIADADQGTSVQ
                     EDVAHKVAGIQAQSEALSEANALIRGIADQTNLLAMNAAIEAAHAGEAGKGFAVVSDE
                     IRKLASTSLDQSNSISKLLSGIHSLIEEVAQASSLSGENFSRINGKISGISVLVQELK
                     GSMEEQNAGSQEILHTITEIKSNCRTLTDDSTQSKQQSGVLYAEVESLRTGAADIRGK
                     TEKAREIATRLQSVATQFSKATKDTAQNIEA"
BASE COUNT          453 a          481 c          542 g          515 t
ORIGIN      
        1 atagccggga acgccgcatc ggcaatttta agcgcatcta tcgacatttc cccgcccgtt
       61 gtggtacggg gagaaaatca ccagatagcc tggcccaaaa ccatgccggt tgtagctatt
      121 ccatttacca cctcttgtgg accttttgag gtcgacgttt gttttaagca taaataatga
      181 agcttccggg gagggttttt gatgaaggtg ttacccaaga gtttgcgggt acggtttgtc
      241 ctgttgtttt ctctgtttat agccgtcttg tgtgtggtgt ccggctggct ttctgcaaag
      301 gccatggtgg atactgccac gagtcttttt tccgagaaag gcgccccgct ttcgcgtgag
      361 gttgcttccc gcttggaccc acagcggtat caggcgctgg tgtcttctct tgacccctta
      421 gacccctatt attccgatac gcatctgtgg atgcgggagc gtaaagaaga aagcggttgc
      481 acctttttat atacgatgtc ccggactgag gatggggtat accgctatat catcgacggt
      541 agttccgcca tggatgatga agaaaacttt tcgcccctgg gcgccgagga agacgcggag
      601 tcctttggcc cggcctttgc aagggctatg gaccagggcc ttccttctca ttcccggctg
      661 gtgtttcagg aaggctgggg ctggcttatc tcggtatact atcccatcaa gaacagcgca
      721 ggcgcggtga ttggcgttgt tggctgcgac tttgcggcag aagccctgcg tcatgatata
      781 agccggtatg tgctccggca ggtattggtg gcggggattt gccttgttgc cggcattgtg
      841 ctcctgctct ttttaaccaa tttagtcttt gctcctatca agttgatgtc ggctccgatg
      901 agttcaattg ccgacggctc gggcgattta acggttacta ttccggttcg tgcgcaaaac
      961 gagatatctg tattggcaaa cggttttaat cgctttgtct cttcccttcg cgatatggtt
     1021 gtttctactc gcggtgcggt tgataacctt acggtagcag gtcaaaccct ggcagaaaat
     1081 tcccgccagg caagcatgtc cgtagaggcg attaccggag agatcgaagc tatccgtacc
     1141 ttggctggca ggcaagacca aatgactata gaaacctttg aaagtatctc ggcccttgag
     1201 cgtcggcttg ccgagttgga caaccaggtt tcttctcaaa cagacaagct taacagctcg
     1261 tttgccgcca tcgaggagat gacggcaaat attactgcga taaataccac catcgagcgt
     1321 atggtaagcc agtataatga attgattgcc gatgccgatc agggcacgag cgttcaagaa
     1381 gatgtggctc ataaggtcgc ggggattcag gcccagtccg aagccctctc cgaggctaac
     1441 gccttgattc gcggtattgc cgatcaaact aatttacttg ccatgaacgc cgcaatagaa
     1501 gccgctcatg ctggcgaggc aggaaagggc tttgcggttg tctcggacga gattcgcaag
     1561 cttgcctcta cttcccttga tcagtctaat tctatcagca aacttctttc ggggatccat
     1621 tcgcttatcg aagaagttgc ccaggcctcg agtctttccg gcgaaaattt ttctcggata
     1681 aacggaaaga tttccggtat aagtgtcttg gttcaggaat taaagggttc aatggaggag
     1741 caaaacgcgg gctcgcagga aattcttcat acgattaccg agataaaaag taattgcagg
     1801 accctgacgg acgattccac tcagagcaag cagcaatcag gggttttgta cgcagaggta
     1861 gagtctttgc ggacgggcgc agcggacatt cgcggaaaaa cagaaaaggc gcgagagatt
     1921 gcgacccggc tccagagtgt tgctacgcaa ttctctaaag caaccaaaga taccgcgcag
     1981 aatattgagg c
//
LOCUS       DAOQXQ010000342         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66480_length_1991_cov_2.943698, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000342 DAOQXQ010000000
VERSION     DAOQXQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66480_length_1991_cov_2.943698"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..546
                     /locus_tag="PKH81_06135"
     CDS             130..546
                     /locus_tag="PKH81_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ97649.1"
                     /translation="MKHLTRELWFTTRKEREFINITPTIEDLVRDSSVKEGLCLVNAM
                     HITASVFINDDEGGLHQDFDEWLEKLAPHEPVSAYRHNVGEVNADAHMKRQIMGREVV
                     VAITGGALHLGPWEQIFYGEFDGKRKKRVLVKIIGE"
     gene            complement(617..>1991)
                     /locus_tag="PKH81_06140"
     CDS             complement(617..>1991)
                     /locus_tag="PKH81_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ97650.1"
                     /translation="FQSADYKTKEFEGWKFTIQASAEDCTGCGLCVQQCPAKSKEDPS
                     VKAINMKNFAEHRAEESENFKFFMSLPDPEASKLNLSNAKFIAMKRPLFEFSGACAGC
                     GETPYVKLLSQLYGDRAVIANATGCSSIYGGNLPTTPYSKNAQGKGPAWSNSLFEDAA
                     EFGFGMRLTSDKLAEYAREVAEQVKDKGIATATIDKILANPQADDTAIETQRKDIESL
                     KAELAKASDPLAKELASLTDHFVKRSVWIIGGDGWAYDIGFGGLDHVLASGRNVNVLV
                     LDTEVYSNTGGQMSKATPFGAIAKFAASGKATSKKDLGMIAMTYGYVYVARVSMGANM
                     NQVIKAFREAESYDGPSIIIAYSHCINHGINMEYGMTNQKEAVTCGLWPLYRYDPRLA
                     DEGKNPFQLDSKDPDFNLADFMYKEVRFKSLKAADPARADMLLKKAEAKAKRQWQEYK
                     YLAERTF"
BASE COUNT          498 a          410 c          512 g          571 t
ORIGIN      
        1 gaaatggagc gatctttgtt atctgccggg tttaccaagt atacctttta tggagacttt
       61 aatggctctt cgtggaaacc agattcatta ttgctcattg gggtgtgttc ctaagtaaag
      121 gagaaaggct tgaagcactt aactagagag ttgtggttta ctacacgaaa agaacgagag
      181 tttattaata ttaccccgac aatagaggat ttagttcgtg attcgtctgt taaagaagga
      241 ttgtgtttgg taaatgccat gcatattact gcaagtgtgt ttattaatga tgatgaaggt
      301 ggtttgcatc aagattttga tgaatggttg gaaaagctgg caccccatga gccggtttct
      361 gcctatagac ataatgttgg tgaagtaaac gcagatgccc atatgaagcg tcaaataatg
      421 ggtagagagg ttgttgttgc tattactggc ggtgcattgc atctaggacc atgggagcag
      481 attttttatg gagaatttga tggaaaacga aagaagcggg tgttggtaaa aattataggt
      541 gaataaaaaa aagccttccg gtaatccgga aggctttttt aggtcctaag accttaatag
      601 cttgtaagcg agactattaa aaggttcgct ctgcaaggta cttgtattcc tgccattggc
      661 gctttgcttt ggcttcggcc tttttaagaa gcatgtcggc gcgagctggg tcagcggcct
      721 tgaggctctt aaagcgaact tctttataca taaagtccgc gaggttaaag tcaggatcct
      781 tgctgtcaag ctggaagggg ttctttcctt catcagcaag acggggatca tagcgataca
      841 ggggccatag accgcaggtt actgcttcct tctggtttgt cattccgtat tccatgttga
      901 taccatggtt gatacagtgg ctatacgcaa tgatgattga cggaccgtcg tagctttccg
      961 cttcgcggaa tgccttgata acctggttca tgtttgctcc catggaaaca cgggcaacat
     1021 atacataccc ataggtcatg gcgatcatac caaggtcctt cttgctggtt gctttgccgc
     1081 ttgcagcgaa cttggcaatg gctccaaagg gggttgcctt ggacatttgt ccaccggtat
     1141 ttgaatacac ttcggtatca agaacaagga cgttaacatt acggccggag gcgagaacat
     1201 gatcaagacc gccgaaaccg atgtcgtatg cccatccgtc accaccgata atccaaacag
     1261 agcgcttaac aaagtggtct gtaagggacg caagttcctt ggcaagagga tcggaggctt
     1321 tggcaagttc tgccttcaga ctctcaatgt ccttgcgttg agtttcaatt gcggtatcgt
     1381 cagcctgggg atttgcgaga attttatcga tagttgctgt cgctattccc ttgtctttta
     1441 cctgttcggc aacttcccgg gcatattccg cgagtttgtc gctggtaagg cgcattccaa
     1501 aaccaaattc tgctgcatct tcaaagagag agtttgacca ggcaggaccc tttccctggg
     1561 cgtttttgga atatggagtg gtagggaggt ttcctccata gattgaggaa caaccagtgg
     1621 cattggcgat tactgctctg tccccgtaaa gctgggaaag gagcttaaca tatggtgttt
     1681 caccacatcc tgcgcaggca ccggagaact caaagagcgg tcgcttcatc gcgatgaact
     1741 tcgcgttgga aaggttgagc ttggaggctt ccggatcggg aagagacata aagaacttga
     1801 aattttcaga ttcttctgca cggtgttcag caaagttttt catattgatt gctttaaccg
     1861 aaggatcttc tttgctcttg gctgggcact gttgcacaca aagtccgcaa ccggtgcagt
     1921 cttctgcaga ggcctgaatg gtaaacttcc atccttcgaa ctctttggtc ttgtaatctg
     1981 cactctggaa g
//
LOCUS       DAOQXQ010000343         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66526_length_1990_cov_3.432558, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000343 DAOQXQ010000000
VERSION     DAOQXQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66526_length_1990_cov_3.432558"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1488
                     /gene="rfbH"
                     /locus_tag="PKH81_06145"
     CDS             145..1488
                     /gene="rfbH"
                     /locus_tag="PKH81_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNQ97651.1"
                     /translation="MPNAPSKDYLKDELLQKVREYYQAVHEPQTKTSFLPGESRVNYA
                     GRVFDAEELVNLVDSSLDFWLTHGEYCDRFEKALAEYLSVKWSLLVNSGSSANLLAFM
                     ALTSPLLKERRIQRGDEVITVAATFPTTIAPIIQYGAIPVFADVELSTCNIDIESMEK
                     ALSDKTKAVMIAHTLGNPFDLEYVTEFCRKHNLWLIEDNCDALGSTYKGEKTGTWGDI
                     GTSSFYPPHHMTMGEGGAAYTNNPLLKKIMLSLRDWGRDCWCSSGKDNTCGKRFSQQH
                     GALPFGYDHKYVYSHFGYNLKATEMQAAIGIAQLQKLPFFIEKRKKNFEHLAERLKDL
                     ERFFLLPRAAQDSDPSWFGYLITLKDETLFTRNALVEFLEKRNIQTRNLFAGNIVRHP
                     AAAYLQEGKDYRIADTLEKTDKIMNDSFWLGVYPGMRQEALKYVTESIHEFIDRY"
     gene            1466..>1990
                     /locus_tag="PKH81_06150"
     CDS             1466..>1990
                     /locus_tag="PKH81_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ97652.1"
                     /translation="MNSLTDIKRTILLTGATGFLGSHLLERFIAKGERVIALKRSTSS
                     IHRVAHLLDKAIFYDIDKTTLDEVFTKNQIHKVIHTACCYGRRRENASEILLANLIFP
                     LRVLEASKKSQVRVFLNTGTLLKREVSPYALSKAQFTDWLPSYAEEMRIVDLRMEYMY
                     GPGDSPEKLLPYVLQ"
BASE COUNT          572 a          489 c          471 g          458 t
ORIGIN      
        1 gcagataccg ctttccacaa gaccgctcgt tggtacaaaa cccagtacga aacgggccat
       61 ctttttaccg aacaagattg ggaagaatac attggagatg cccgcgttgc gggagcttcc
      121 tggatgtttt gaaggagaca gaatatgcct aacgcgcctt cgaaagatta tctgaaagat
      181 gaattgttac aaaaagtcag ggaatactat caagccgtcc acgaaccgca gacgaaaaca
      241 tcattcttgc ctggagaaag tcgcgtaaac tatgcgggca gggtcttcga cgcagaagaa
      301 cttgtcaacc tcgtagacag ttcgcttgac ttctggttga cgcacggcga atactgtgat
      361 cgcttcgaga aagcattggc agaatacctc agcgttaaat ggtctttgct tgttaattct
      421 ggatcatccg cgaatctact ggccttcatg gctcttacct ctccactact aaaagagaga
      481 cgaatccaac ggggagacga agtgatcact gtagccgcca cattcccgac gacaatcgcc
      541 cccatcattc agtacggcgc tatcccggtc ttcgccgacg tggaactcag cacatgtaac
      601 atcgacatcg aaagtatgga aaaggctctg tcggataaaa ctaaggctgt tatgatcgca
      661 catactttgg gaaacccttt cgacctcgaa tacgtcaccg aattttgccg gaaacataat
      721 ctgtggctta tcgaagataa ttgcgatgcc cttggatcta cctataaagg agaaaaaacc
      781 ggaacctggg gcgacattgg cacgagcagt ttctacccac cgcaccatat gactatgggt
      841 gaggggggag ccgcctacac caacaaccct ctactaaaaa aaatcatgct atctcttcga
      901 gattggggaa gagattgctg gtgttccagc gggaaggaca acacttgcgg gaaaagattc
      961 tcgcagcagc acggggcact cccgttcggt tacgatcaca agtacgtcta cagccacttc
     1021 ggctacaacc taaaagctac agaaatgcaa gctgccattg gaatagccca actacaaaaa
     1081 ctcccgttct tcattgagaa gaggaaaaag aatttcgagc atttagccga aagactcaaa
     1141 gatcttgaac gcttctttct gctccctcgg gcggcgcaag atagtgatcc gagctggttc
     1201 ggctacctaa tcaccttaaa agatgaaacg ctttttacac gcaacgcgct cgtagagttt
     1261 ttggagaagc ggaatatcca gacgcgcaac cttttcgcag gcaatatcgt ccgtcatcca
     1321 gcggctgcgt atttacagga aggaaaggat taccgcatag ccgatacgct ggaaaaaacg
     1381 gacaagatca tgaacgacag cttctggctg ggtgtctacc caggtatgcg tcaagaagcg
     1441 ttgaagtatg tgacggagag cattcatgaa ttcattgacc gatattaaaa gaacgatcct
     1501 tctgaccggc gcgacgggct ttcttggaag ccatttgctt gaaaggttca ttgccaaagg
     1561 agaaagagtc atcgcgctca aacgttctac atcgagcatt caccgtgtag cgcatctact
     1621 tgacaaagcg atcttctacg atatagacaa aactactctt gatgaagtat tcacaaagaa
     1681 tcagatacat aaggtaatcc ataccgcgtg ttgttatggg cgaagacggg agaatgcatc
     1741 ggaaatattg ctcgccaacc tcatctttcc cctgcgcgtc cttgaagcat ccaaaaagag
     1801 tcaagtaagg gtctttctga ataccggcac acttttgaag cgtgaggtaa gtccctacgc
     1861 cctctccaag gcgcagttca ccgattggct tccttcttac gccgaggaaa tgcggatcgt
     1921 agatctgaga atggagtata tgtacggtcc cggagacagc cctgaaaaac ttctacccta
     1981 cgttttgcag
//
LOCUS       DAOQXQ010000344         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_66801_length_1985_cov_3.718135, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000344 DAOQXQ010000000
VERSION     DAOQXQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66801_length_1985_cov_3.718135"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="PKH81_06155"
     CDS             complement(<1..1916)
                     /locus_tag="PKH81_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNQ97653.1"
                     /translation="MTGSVAQGYAARLNLPVYQEVMAAAAAVRRMHPQARMVLDIGGE
                     DSKVFSFTPQGSVDFRMNGNCAGGTGAFLDQLTELLRLENQSLEELARLGSRVYPIAG
                     RCGVFAKTDVQNLLSRHISKPDIALSVLHTLCNQFCATLIKKRDNDSSVIFCGGPFHF
                     IPLLQEIFCTHLQLEPQAVINSEHTLFFPAIGAALHSATSSTSVSFESLLLNLNNFCP
                     EQNAAKERVLCKDIQAFTSWQASRFTPTPKTTLRDADDSAFYVGIDSGSTSTKMILLD
                     NKARIVDSFYRFNNGEHVRTVEQGLRYFKSNLASYPKSTIITSAAVTGYGEDLVRGFY
                     AIKHSLVEPLAHYKGALSLGIIPDTILDIGGQDMKAIFLSNGEITHIELNEACSSGCG
                     SFFQNFATSYGCSLEEFSQKAALAQDPCYLGSRCTVFMNSLVKQALHNGQDIDSIAAG
                     LAYAVARNAIEKMLKVHTQDLGETIVVQGGTFLNPAIHKAFENYLGRTLICPDISGLM
                     GAYGAALHAKERTSVEDINPGKDQFPLSSRVEDITSDTLHCKGCAHNCLVTRSLFPDG
                     TYLYTGNRCQKIWGRSAGASDDHLVALKNRLLSIPSQHTAPDSSVTIGIPRVLLFYEH
                     FPFWHELFSRCGFT"
BASE COUNT          525 a          436 c          519 g          505 t
ORIGIN      
        1 atagtaaaac cgcagcgaga aaaaagctcg tgccaaaagg gaaagtgttc ataaaacaat
       61 aatacccggg gaattccgat tgtaaccgaa gaatcgggtg ccgtgtgctg gcttggaatc
      121 gaaagcaagc gattttttag cgcgaccaaa tgatcgtcgc ttgcgccagc agagcggccc
      181 catatttttt gacagcggtt tccggtatat aaataagtgc catccggaaa taaagatctt
      241 gttaccaaac aattgtgagc acagccctta cagtgaagtg tatcgcttgt aatatcttca
      301 acacgcgagg ataaggggaa ttgatctttg ccaggattga tatcctcgac ggaagtacgt
      361 tctttggcgt ggagtgcggc cccataggcc cccatcaagc cgctgatatc tggacagatc
      421 aaggtgcggc caagatagtt ttcaaaggcc ttgtgaatcg cgggattcag gaaggttccg
      481 ccttgaacaa ctattgtttc gcccaggtcc tgtgtgtgga ctttaagcat tttttctata
      541 gcgtttctgg caactgcata ggccaggcct gcggcgatgc tgtctatatc ctgtccgtta
      601 tgcagggctt gttttaccag ggagttcata aacacggtac agcgggagcc taaataacaa
      661 gggtcctggg caagggccgc tttttgggaa aactcttcca gagagcagcc ataggaagtt
      721 gcaaagtttt ggaaaaaact gccgcagccc gacgagcacg cctcgttcaa ttcaatgtgg
      781 gttatctctc cgtttgaaag aaagatagcc ttcatgtcct ggccgccaat atccaggatg
      841 gtgtccggaa taattcccag ggaaagggct cccttgtaat gagcaagggg ttcaacaagg
      901 ctatgtttga tagcgtaaaa gccgcgaacc aagtcctctc cgtatccggt aaccgcggcg
      961 gatgtaatga tggtagattt ggggtacgaa gcaagattgc ttttgaaata acggagaccc
     1021 tgttcaacgg tgcgcacatg ctctccatta ttaaaacggt aaaaagaatc gactatccgc
     1081 gccttgttgt ccaatagaat catttttgta cttgtagaac ctgaatcaat gcctacataa
     1141 aatgcgctgt cgtcagcatc gcggagggtg gttttgggag ttggggtaaa tcgactggct
     1201 tgccaactgg taaacgcttg aatgtcctta cagagtacgc gctcttttgc tgcgttttgt
     1261 tcaggacaga agttgttcag attcaagaga agtgattcga aagaaaccga ggtgctggat
     1321 gttgcactgt gtaaggcagc gccaatagcc gggaagaaaa gagtgtgttc ggaattgata
     1381 acggcctgag gctcaagttg taagtgtgtg caaaaaattt cttgcagcaa aggaataaaa
     1441 tgaaaagggc ccccacaaaa aataacgcta ctgtcgttgt ctcttttttt tatcaaggta
     1501 gcgcaaaact ggttacacag ggtgtgtaat accgaaaggg caatgtctgg ttttgatatg
     1561 tgtctactga gtaaattctg tacatctgtt ttggcaaata ccccgcagcg acctgctatt
     1621 ggatagacgc gggaaccgag tcgtgcgagc tcttccaaag attgattttc cagacggagc
     1681 aattcagtca gttgatcaag aaaggcaccg gtgcctccag cgcaattacc gttcattcta
     1741 aaatcaaccg aaccttgcgg tgtaaagctg aataccttgg agtcttcgcc gccgatgtcc
     1801 agcaccatcc tggcttgggg atgcatccgg cgaacggccg ctgcagccgc catgacttcc
     1861 tgatatacgg gtagattaag gcgtgcggcg taaccctggg cgacggagcc ggtcataccg
     1921 acctttgcct agccatagct ttggtgtaga tgatgcagta tctcttgtaa ggattcattt
     1981 attgc
//
LOCUS       DAOQXQ010000345         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_67031_length_1981_cov_3.481308, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000345 DAOQXQ010000000
VERSION     DAOQXQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67031_length_1981_cov_3.481308"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..>1981)
                     /gene="htpG"
                     /locus_tag="PKH81_06160"
     CDS             complement(190..>1981)
                     /gene="htpG"
                     /locus_tag="PKH81_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNQ97654.1"
                     /translation="LKYLTLSDESYKGVAFTPRVDISFDEEKKTLTVRDTGIGMDDQD
                     IISNLGTIARSGTKAFIEQLASDAKKDSNLIGQFGVGFYSAFMVASKIEVISKKAGDN
                     RVWKWISDGKGSYEMEETDDTAFPILEGVGEGLNGTCVVMYLNDDDKEFATRWKIEDI
                     VKRYSDHIAFPIYLHYVKKEYDDKGKVKSEAPVAEQINDASAIWQKSKNDLKEEDYKS
                     FYKSLSHDSEDPLLYVHTKAEGTQEYTTLFYVPAKAPFDMFNADYKSGVKLFVKRVFI
                     TDDDKELLPVYLRFVRGIIDSEDLPLNVSREILQQNRILTNIQSASVKKLLGEFKRLA
                     DSDKDKYNTFIGEYNRPLKEGLYGDYAHREELAELVRFKTTDEGVADGWTSLADYASR
                     MKSDQKAIYYITGGDEKTLRQSPHLEAYRAKGFEVLIMADEIDEIVVPSLGKYKEWEL
                     KAANRAGSDEELAGDGDKKAREEKEKAFKDAVEKVKKALGERVKDVRLSKRLADSPSC
                     IVVDENDPSLQMERMMKAMGQQGFAEVKPILEINADHVLVKKIQDSSDDDFVADVSSV
                     LLDQALIVEGSELKDPADFVKRLNRLLSRS"
BASE COUNT          480 a          512 c          450 g          539 t
ORIGIN      
        1 gctgcggacg ttgatcggga acctgtagat cctcggaaaa acccattccc gcgactataa
       61 gcgcgagaag taagaccagc gcgagtttcc tcataaaggc ccccttataa aaaaagcata
      121 cccccgaacc ggaaccctgt ccagtgcggg ggtacaactc atgagtcaag aagccggttt
      181 tcccgaatac tacgagcggg aaagaagtct gttaagccgt ttaacaaaat cggctggatc
      241 cttaagttca gaaccttcta caatcagagc ctgatccaga agaaccgaag aaacatccgc
      301 cacaaagtcg tcgtctgaac tgtcttgtat cttcttgacc agtacgtgat cggcgttaat
      361 ctccaatatt ggtttaacct cggcgaaacc ttgctgaccc atagccttca tcattcgttc
      421 catctggagg gaaggatcat tttcgtctac aacgatgcac gatggagagt ctgcaaggcg
      481 tttggaaaga cgaacatcct ttacccgctc tccaagagcc ttctttacct tttctaccgc
      541 gtccttaaag gccttttctt tttcttcgcg agctttttta tccccgtctc cggcaagctc
      601 ctcgtcgctt cccgcacggt tagcggcctt aagttcccat tctttatact taccaaggga
      661 gggaacgacg atttcgtcaa tctcgtccgc cataatgagt acttcaaatc ccttggcccg
      721 atacgcctca agatggggac tttgtcggag ggttttttca tcgccaccgg taatgtagta
      781 gatagccttc tgatcgcttt tcatccgtga agcatagtcg gcaaggcttg tccatccatc
      841 agcaacgcct tcgtcagtgg tcttgaaccg aacaagctcg gcaagctctt cgcgatgggc
      901 gtagtcgccg tacagacctt ctttaagcgg tcgattatac tccccgataa aggtgttata
      961 cttgtcctta tcgctatcgg caaggcgttt aaactctccc agaagctttt taaccgaagc
     1021 gctctggata ttggtaagaa tgcgattttg ctggagtatc tcccggctta cgttaagcgg
     1081 caggtcttcg ctatcgataa taccgcgcac aaagcgaagg tacacgggca gcagttcctt
     1141 gtcgtcgtcg gtaataaata cgcgctttac aaagagcttt accccgctct tatagtcggc
     1201 attgaacata tcaaagggag cctttgccgg cacgtaaaag agggtggtat attcctgagt
     1261 tccctcggct ttggtatgaa catacaagag cggatcttca gaatcatgag aaagagattt
     1321 ataaaaactc ttgtaatcct cttctttaag atcattcttg ctcttttgcc aaatcgcgct
     1381 cgcgtcgtta atctgttctg cgacaggagc ctctgatttg acctttccct tatcgtcata
     1441 ttccttttta acgtagtgga ggtaaatggg aaaggcgata tgatcggagt agcgtttgac
     1501 aatgtcttcg atcttccagc gagtggcaaa ttccttgtcg tcatcgttca ggtacattac
     1561 cacacaggta ccattaaggc cttctcccac accttcaagg atggggaagg cggtatcgtc
     1621 ggtctcttcc atctcgtaag agcccttacc gtcggatatc cacttccaca cgcggttatc
     1681 tcccgccttt ttggaaatga cctctatctt ggatgcgacc atgaaggctg agtaaaaccc
     1741 aacaccaaac tgaccgatca ggtttgagtc ctttttcgca tcggatgcca gctgttcgat
     1801 aaaggccttg gttccacttc gagcaatcgt tcccagattg gaaataatgt cctggtcgtc
     1861 cataccaata ccggtgtcgc gaacggttag ggttttcttt tcttcgtcaa aggaaatgtc
     1921 aacgcgcggg gtaaaggcca cgcccttata cgactcatcg gaaagggtaa ggtacttgag
     1981 t
//
LOCUS       DAOQXQ010000346         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_67351_length_1975_cov_3.390104, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000346 DAOQXQ010000000
VERSION     DAOQXQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67351_length_1975_cov_3.390104"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..889
                     /locus_tag="PKH81_06165"
     CDS             161..889
                     /locus_tag="PKH81_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNQ97655.1"
                     /translation="MKENNTVSESRIIWRSPSALVAYKNAGEICEALEPESRASLVSI
                     IRNELALSGHVDLEQVTAVHRIDRPVTGCTLFALTAESASALSRQFSQGTIRKRYLAI
                     VELSSFLTDTQSGMLEHWIRFDSKTQKSRILPLCETPRSPGWKKASLSWRLAGKGDHY
                     AFLEIDPHTGRTHQIRAQLAFEGIPIKGDLKYGARRSERSGGIRLHAYMIQFTDISSG
                     ERITVQSSCRNPDALWSAFPGLDS"
     gene            complement(886..>1975)
                     /locus_tag="PKH81_06170"
     CDS             complement(886..>1975)
                     /locus_tag="PKH81_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNQ97656.1"
                     /translation="VSLLETIENPCFGVCERVTCYFDCHGKSYAFETVVRDNKQRNII
                     VDPPAQLLRSLQRKFVRVKKPRNVQVIFHLSNEEIKLDYPVCPEYVSVEEYDVKSKIQ
                     GKNLPEIVSQFKQSISDTCSENTIIMFRNRKPSCFEEELISKTGKVLFVPSTGASLPK
                     KDPYPEGRIITEEQEEKFEDPNFFIEGSRFEKLLKEKKEKGISSEIWCPIIYYQYVVG
                     YIYVTDRSGGSFDVSMVDYLWDFSRILAFNLKQTGYFSGEKKTEAAPGHQAQVLDMSP
                     GGTLISLPRSEIRTPIKEGSVFSVDVLMGDKTVNCSVKVVRRYEESEAISYGMTFLNL
                     SSQDVMQIYEGLYRKPYNERDSLAFEQS"
BASE COUNT          543 a          407 c          409 g          616 t
ORIGIN      
        1 tgcgtattct tgcttttaag attcaagcag gtattcttcc tacagcacga aagaaaatgg
       61 gacagtcaat gaatcgtgta tctaggataa gttcttttga ttcaaattct tttgcttatg
      121 caaaattaca agcacagagg ctcctggagg agattcaata atgaaagaga ataatactgt
      181 atctgagtca aggattattt ggcgtagtcc atctgctttg gttgcgtata aaaacgcagg
      241 ggagatttgc gaagcgcttg agccagaatc tcgtgcttct ctggtatcta ttatccgcaa
      301 tgaacttgct ttgtctggac atgtggatct tgaacaggta actgctgttc accggattga
      361 tagaccggta accggctgta cgctttttgc tcttaccgct gaatctgcat ctgctttgtc
      421 tcgtcagttt tcgcaaggaa caattcgcaa acgctatcta gctattgttg agctctcttc
      481 atttcttaca gacactcaat caggtatgct ggaacattgg atccggtttg actcaaagac
      541 acaaaaatcg cgtattctac ctctgtgcga aacacctcga tccccgggat ggaaaaaagc
      601 atctctttca tggcgattgg ccgggaaggg tgatcattat gcatttttag aaatcgatcc
      661 acatacgggt agaactcatc agatacgcgc tcagttggcc tttgagggaa taccgataaa
      721 aggagattta aagtatgggg cgcgcagaag cgaacgctct ggaggaattc gacttcacgc
      781 gtatatgata cagtttacag acatctccag cggggagcga ataacagttc agtcttcttg
      841 tagaaatcca gatgccttat ggagtgcctt tcctggactt gattcttagc tttgctcaaa
      901 ggctagagag tctcgctcgt tatagggttt cctgtaaaga ccttcatata tttgcattac
      961 atcttgcgaa gataggttca aaaaggtcat tccatacgaa atcgcttctg attcttcata
     1021 gcgacgaaca acctttaccg agcagttaac tgttttatct cccatcagaa catcaacgga
     1081 aaacaccgat ccttctttga taggtgttct aatttcagat cggggaaggg aaataagtgt
     1141 tcctccggga ctcatgtcca acacttgggc ttgatgaccg ggggctgctt cagttttttt
     1201 ttcaccagaa aaataacctg tttgttttaa gttaaaggca agtattcggg aaaaatccca
     1261 taggtaatca accatggaaa catcaaagga tcctcccgat cgatcagtta catagatata
     1321 tccaactaca tattgataat agatgatggg gcaccatatc tcagatgaaa tacctttctc
     1381 ttttttttct tttagaagct tttcaaaccg tgacccttca ataaagaaat taggatcttc
     1441 gaatttttct tcttgttctt cagtgattat tcttccttcg gggtagggat cttttttggg
     1501 taaactggcg ccagtggaag gtacaaatag aacctttcct gttttactga ttaactcttc
     1561 ttcaaaacaa gaaggctttc tatttctgaa catgataata gtattctcgc tacatgtatc
     1621 gctaatgctt tgcttaaatt gcgatactat ttccggcaga tttttcccct gtattttgga
     1681 tttcacatca tactcttcaa cgctcacata ctcagggcaa accggataat ccagtttgat
     1741 ttcttcattc gataaatgaa agattacttg aacatttctt ggttttttaa cgcgaacaaa
     1801 tttcctttga aggcttctca agagctgtgc tggcggatct acaataatgt tgcgttgctt
     1861 gttatctcgt actacggttt caaaggcata tgatttgcca tggcagtcaa aatagcaggt
     1921 aactcgctca cacacgccaa aacagggatt ttcgattgtt tccagaaggg agacg
//
LOCUS       DAOQXQ010000347         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_67374_length_1975_cov_2.863021, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000347 DAOQXQ010000000
VERSION     DAOQXQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67374_length_1975_cov_2.863021"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..799
                     /locus_tag="PKH81_06175"
     CDS             104..799
                     /locus_tag="PKH81_06175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ97657.1"
                     /translation="MSDLEKKTGLPCLFCGNTGTEAIVLPDREYHRCLSCQGITLDQR
                     FRLSASAEQERYRLHINTLDDRGYRSYLTHFLDTVLDFYRTRFSPDIKTLRLFDFGSG
                     PTPALVELARERGIDTRFWDPFFAPEGKNFIGGADIVTCLEVAEHFYEPRTNFLHLAG
                     ELRPGGLLAIGTLLCDNKDFPSWWYRQDSTHVSFYSKQALLALGREVGLVLESAKDDR
                     IFLFTTKDNPSSP"
     gene            complement(768..1358)
                     /gene="rdgB"
                     /locus_tag="PKH81_06180"
     CDS             complement(768..1358)
                     /gene="rdgB"
                     /locus_tag="PKH81_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNQ97658.1"
                     /translation="MKIYLASGNRHKRDELMRIFYPHTIVITSDEGIAFDPEETGVTY
                     FENALIKARSLYEITGEAALADDSGISVDALEGRPGIHSARFGSENGTKLTDLQRNNL
                     LLSHMQGKTDRTARFVCNMVLYLGPERFISAQETMEGTLMESAQGTGGFGYDPVLYIP
                     EAGCTVAELSPETKDALSHRGKAARKIRAMMDCLSS"
     gene            complement(1379..>1975)
                     /locus_tag="PKH81_06185"
     CDS             complement(1379..>1975)
                     /locus_tag="PKH81_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97659.1"
                     /translation="DQARQKVYQTEEEARILSITAEQYNSGNWNDYLSELETHYSDEE
                     NSQNKKPKREKSAKQKEIEARRLLLLACKNAHLLSDSQIEAIARMAGFSQEFLKSRID
                     LVRQTAALAQDRIRLNREKEYTYYLRSQKCLHEMKELEEGPLFEQIEKEYQYCLKRIA
                     DLRLTISHQPASPSNRLLAKKLGFCRTVVDSALAREER"
BASE COUNT          449 a          464 c          469 g          593 t
ORIGIN      
        1 catccctgta gtggagaaaa gatcgaatcc gtggtagttt cgacgaaacg tccacaggac
       61 aatatcttcc ccgtttcttt tatcggcatg aggatggcaa tacttgagtg atttagaaaa
      121 aaaaaccggt ttaccctgtc ttttttgcgg aaataccggg acagaagcga tagtccttcc
      181 tgatcgggaa taccaccgat gcctctcatg ccaggggatc accctggatc agcgattcag
      241 gctttctgca agcgcagagc aggagcgcta ccgtttacac ataaataccc tggatgatag
      301 ggggtatcgc tcgtatttga cccatttttt ggatacggtt cttgattttt atcgcacccg
      361 cttctcacca gatataaaaa ccttacgtct ttttgatttt ggtagtgggc caaccccagc
      421 ccttgtcgaa ctcgcacggg agcggggtat cgatacacga ttttgggatc ctttttttgc
      481 cccagaagga aagaacttta tcggaggagc ggatattgtt acctgtcttg aagttgccga
      541 acacttttat gagccacgca ccaatttttt gcatctggcg ggggaactga ggccaggggg
      601 cctactcgct atcgggacac tgctttgcga taacaaggat ttcccttcgt ggtggtatcg
      661 acaggatagc acgcatgtat cattttattc aaagcaagcg ttactcgcac tgggtaggga
      721 agtgggctta gttcttgagt cggcaaaaga tgatcgaatt ttcttgttta cgacgaaaga
      781 caatccatca tcgccctgat ttttcttgcg gcctttcccc gatgagaaag agcatccttg
      841 gtttcaggag aaagctctgc aacagtacat cctgcttcgg gaatatacag caccggatcg
      901 tatccaaaac cacctgttcc ctgtgcactt tccatcagtg tgccttccat cgtttcttgc
      961 gcactgataa aacgctctgg acctagatac aggaccatgt tgcagacaaa tcgtgctgta
     1021 cggtcagttt ttccctgcat atgggataaa agcaggttgt ttcgctgtaa atcggtgagt
     1081 tttgtcccgt tttctgaacc gaaccgggcc gaatggatac ccggccttcc ttcaagggcg
     1141 tctacagata ttcctgaatc gtcggccagg gccgcctcgc cggtaatttc atatagggag
     1201 cgagccttaa taagggcatt ttcaaaataa gtcactcctg tttcttccgg atcaaaggca
     1261 atcccctcat cagaggtgat gacgatagta tgcgggtaaa aaatgcgcat cagttcatct
     1321 cttttgtggc gattacccga agctaggtat attttcatag gtatgcctac cttacccgtt
     1381 aacgctcttc cctcgcaagg gcactgtcta ccacagtacg gcaaaagcct agtttttttg
     1441 cgagcaagcg attgcttgga gaagcaggtt gatgtgatat cgtaaggcgt aaatcggcga
     1501 ttcgcttaag gcagtattga tattccttct ctatctgctc gaataaggga ccttcttcaa
     1561 gctccttcat ctcgtggagg catttttggg atctaagata gtaggtatat tctttttcgc
     1621 gattgagcct gatgcggtcc tgggccaggg ctgccgtttg acgaacaagg tcaattctgc
     1681 tttttagaaa ttcttgcgaa aaccccgcca tccgcgctat tgcttcaatt tgactgtcgc
     1741 tgagtaagtg tgcgttttta cacgcaagca agagtaatcg cctcgcttct atctcttttt
     1801 gcttggcgga cttttctcgt tttggttttt tgttttggct gttttcttcg tcgctatagt
     1861 gcgtttccag ttcagacaga tagtcgttcc agtttccgct attgtattgt tcagccgtta
     1921 tacttaagat gcgcgcttct tcttcggttt gatagacctt ttggcgtgct tgatc
//
LOCUS       DAOQXQ010000348         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_67640_length_1970_cov_3.042298, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000348 DAOQXQ010000000
VERSION     DAOQXQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67640_length_1970_cov_3.042298"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKH81_06190"
     CDS             complement(<1..780)
                     /locus_tag="PKH81_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ97660.1"
                     /translation="MRATINDIAKKAGVSKTTVSFAFNNPDKISGETRDRIMAIASEL
                     GYVPDPVARTLAMRQTGAIGVLLPQSVHEVFHNPYIAEILRGIGYVCDGSDVSLSILS
                     PQNGVLTKTIRNAAVDGIITVGIGPGMSVLELLGQRRMPFVTIDGGKHTNLVNVGIDD
                     EAAAEALMEEVLSRSHRDVAVFMLKRVTLSETGDHFSLTNDMRLNGFGKALDRYGLSF
                     GQYSTARVCHTEATIESGKEAARELLALPRRPTAILCLADIQ"
     gene            complement(790..1614)
                     /locus_tag="PKH81_06195"
     CDS             complement(790..1614)
                     /locus_tag="PKH81_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ97661.1"
                     /translation="MRGTRAIAVHRILLTALMCIVAAVFFYPVVYVLINAFKSDALIT
                     LDPMGLPRQWYPDNFIRAWQAMEFPRVFMNTFIITVVSLSGIILISALAAYKLARTDT
                     RLSWLLYGYFVLALVIPFQIIMVPIAVLSADLHLMNIPGIIVMYWGLGCPTAIFMFHG
                     FIKSVPRELEESAAIDGAGQWYIFFAVVFPLLKTIVATVAVIDALWLWNDFLLPLIVI
                     KQGTIQLAQMIFNGQFLKEYGPMTASLTLSALPIVLFYLALQKYIIKGIAAGAVKG"
     gene            complement(1611..>1970)
                     /locus_tag="PKH81_06200"
     CDS             complement(1611..>1970)
                     /locus_tag="PKH81_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ97662.1"
                     /translation="YLAGLQNIPTDVLEAARIDGASAFQRFRSITLPLLVPAITISFF
                     LTIANALKSFDLIYAITSGSAYTFGTVPVVLDIYFDAFARKQAGLATAKAMLLFSIIF
                     LVTGIQLYVMKKKEVEQ"
BASE COUNT          572 a          486 c          480 g          432 t
ORIGIN      
        1 ttgaatgtcg gcaagacata gaatagcggt aggacggcgt ggcagggcaa gaagttcgcg
       61 ggctgcttcc ttgccacttt cgattgtcgc ttcggtatga caaaccctgg ccgtactata
      121 ttgcccaaac gaaagcccat aacgatcaag agccttgcca aatccgttaa gtcgcatatc
      181 attggttaag ctgaaatgat ctccggtctc ggagagagtt actcgtttaa gcataaacac
      241 agcaacatcg cgatgggagc gggaaagtac ttcttccata agggcttcag cggcggcctc
      301 gtcgtcaata cctacattaa ccagattagt atgtttgccg ccgtctattg taacaaaggg
      361 cattcgccgt tgtcccaaca attcaagaac cgacattccc ggaccaatgc caaccgtaat
      421 aatcccgtca accgccgcat tcctgatcgt cttggtaaga acgccatttt ggggagaaag
      481 gatactcaag gacacatcgg agccgtcgca cacatagccg attccccgca gtatttccgc
      541 gatataggga ttatgaaaaa cctcgtgcac tgactgtgga agcaacactc caatggcgcc
      601 ggtttggcgc atcgcaagag ttcgggcgac cggatcgggc acatagccaa gctcgctcgc
      661 gattgccatg atgcgatccc ttgtctcgcc cgaaatcttg tccggattgt taaaggcaaa
      721 agaaacggtt gtcttggaaa cgcccgcttt tttggcaatg tcgttaattg tcgctcgcat
      781 atctatccgc taccccttaa ccgcaccggc ggcaataccc ttgatgatat atttttgcaa
      841 ggcaagataa aagagcacaa tcggaagtgc ggacagggtc aaagatgcgg tcatcggacc
      901 gtactcctta agaaactgtc cgttaaagat catctgggcc agttgtatcg ttccttgctt
      961 aatgacaatg agcggcagga gaaaatcgtt ccagagccac agggcgtcaa ttaccgcgac
     1021 ggtagccaca attgttttta acaggggaaa caccactgca aaaaaaatgt accattggcc
     1081 tgccccgtca atcgcggccg attcttccag ttcacgggga acgcttttga taaacccatg
     1141 aaacataaag atagccgtag ggcatccaag gccccagtac atcacgatga tacccggtat
     1201 attcatcaga tgcaagtcgg ccgacaacac ggcaatcggt accataataa tctgaaacgg
     1261 aattactaaa gcaaggacaa agtatccata caggagccaa gaaagacggg tgtccgtacg
     1321 cgcaagctta taggcggcaa gcgcgcttat taaaataatg ccagatagag atacaacagt
     1381 gatgataaag gtattcataa acacccttgg gaactccatc gcctgccatg cgcgaataaa
     1441 attatcagga taccactgcc ggggaagccc catcggatca agggtaataa gagcgtcact
     1501 cttaaaggca ttaataagaa catacacgac cggataaaaa aagactgccg caacgatgca
     1561 catcagtgcg gtgagcaata tgcgatgtac agcgattgcc cttgtgcctc tcattgttca
     1621 acctccttct tcttcatcac atacagttga ataccggtga ccaggaagat tatcgaaaac
     1681 agcagcatag ccttggcggt ggcaaggcct gcttgtttac gcgcaaaggc gtcaaagtaa
     1741 atatcaagaa caaccggaac cgttccaaag gtataggcag acccggaggt tatggcgtag
     1801 ataagatcga agctctttaa ggcgttcgcg atagtcagga aaaaagagat ggtaatcgct
     1861 ggcactaaca gaggtagcgt tatggaacgg aaacgctgaa aggcgctcgc tccatcaatt
     1921 cgggcagcct cgagtacgtc agtgggaatg ttttgtaagc ctgccagata
//
LOCUS       DAOQXQ010000349         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_67758_length_1968_cov_3.249869, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000349 DAOQXQ010000000
VERSION     DAOQXQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67758_length_1968_cov_3.249869"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKH81_06205"
     CDS             complement(<1..353)
                     /locus_tag="PKH81_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HNQ97663.1"
                     /translation="MIRIKNQKQIDGIRASCKALARLYKLLVPQVQAGMSTADIDDIC
                     VQYIKKIGGKPAWYSQKFPGAACISINEEVIHGIPSKKRIVQEGDLVSLDIGIDLAGY
                     ISDSAVTVPVGKVSK"
     gene            complement(389..1201)
                     /locus_tag="PKH81_06210"
     CDS             complement(389..1201)
                     /locus_tag="PKH81_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNQ97664.1"
                     /translation="MEDCYQILGIPSSASASEIKRAFRRKAKELHPDIPVGGQAVIDR
                     QERMRDLIRAYKVLSDPERRVEFDSLYTIFRKYSPKQEDGGFDYRRWLSAREDPESRA
                     KLIFFDLLHDLEDQAVLEYRKQRDSSPSFMLSGFFSREDFMDCGFILAEELFFREEYY
                     ESFLLLAEVIQLERSKPYFRHFFPEVMLLTRTLLRTHVCGIISDELALDCLETALELG
                     FGKKEDAVFLKLMSMCYDRLGDLSMARLCLEKALALDSSLTGIKELRRRLAV"
     gene            complement(1212..1622)
                     /locus_tag="PKH81_06215"
     CDS             complement(1212..1622)
                     /locus_tag="PKH81_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97665.1"
                     /translation="MAIALVTFLDTPDQHTKAILDSLGSAIRTRGFDLVLVDGTSASS
                     TVAMATFDYIATITRPDSLWRATVPARVEQFWKEAVGIQGKKGCTLVIRHGWRTNAFC
                     RKVMAIMESVGICVDYFEIIRDAGHCSDIAPFIG"
     gene            complement(1636..>1968)
                     /locus_tag="PKH81_06220"
     CDS             complement(1636..>1968)
                     /locus_tag="PKH81_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ97666.1"
                     /translation="VNRIFIDEESREYEQSLEENGRRYLSYAEELGKAKGVKIETELR
                     RGAIWSEVVTMSEERKMDLILLGGQEGETVDSKDIMSSTYRSVMMNARCSVLIVKERM
                     IEQLFKLA"
BASE COUNT          553 a          498 c          390 g          527 t
ORIGIN      
        1 tctttggata ccttgccaac gggaaccgtt accgcgctat cactaatgta tcctgcaagg
       61 tcaataccaa tatcaagtga tacaagatcc ccttcttgca caattctttt tttggaagga
      121 atgccatgaa taacttcttc attgattgaa atacacgcag ctccaggaaa tttttgggaa
      181 taccatgcgg gtttccctcc aatttttttg atatactgga cacatatatc atcaatatct
      241 gcggtactca ttcccgcttg aacctgagga accaacaatt tgtacaagcg agcgagcgcc
      301 ttacaagagg ctcgaattcc atcaatctgt ttctggtttt ttattctaat cacgtctatt
      361 cctttgtagc gtactttctt acggtgtatt ataccgcaag acgccgccgt aattccttaa
      421 ttccggtcaa gcttgaatca agagccaatg ctttttctag acaaaggcgg gccatggaca
      481 agtctcccaa ccggtcataa cacatactca tcagtttaag aaaaacagca tcttcttttt
      541 ttccaaatcc aagttcaaga gccgtttcca gacagtcgag ggcaagttcg tcactgataa
      601 ttccgcacac atgagtgcgt agcagagttc gggttaacag cataacctcg gggaagaaat
      661 ggcgaaaata cggtttactc cgctcaagct gaattacttc cgcaagaagc aaaaatgatt
      721 cgtagtattc ttccctaaaa aatagttctt cggcaaggat aaaaccgcaa tccataaaat
      781 cttcacggct aaagaatcct gaaagcataa acgaaggaga actgtcgcgt tgtttacgat
      841 attcaagaac cgcttgatcc tccaaatcgt gtaataaatc aaagaatatc agcttagccc
      901 tgctctctgg gtcctctcgg gcagaaagcc accttcgata atcaaagccg ccatcctctt
      961 gctttggaga atacttgcgg aatatcgtat acaggctgtc aaattctacc cgtcgctctg
     1021 gatccgagag aaccttatag gcgcgaataa gatcgcgcat ccgctcctgc ctatcgataa
     1081 ccgcctgtcc tccgaccgga atgtcgggat gcagttcctt ggccttacgc ctaaaagcgc
     1141 gttttatctc ggacgcagag gcagatgagg ggataccgag aatttgataa cagtcttcca
     1201 cgtgcgaacc tttacccaat aaaaggcgcg atatcgctac aatggcccgc gtcccgaatt
     1261 atctcgaaat aatcgacaca gatgccaacg ctttccatta tcgccattac cttgcggcaa
     1321 aaagcgtttg tccgccatcc atgacgtata acgagagtgc aacctttttt accttgtatg
     1381 ccaacggcct ctttccaaaa ctgctctaca cgtgcaggaa cggtggctct ccaaagagaa
     1441 tcagggcggg taatagtagc gatataatca aaggttgcca tggcaacagt tgatgaagca
     1501 gatgttccgt caaccaacac aagatcaaaa cccctggtgc gtatggcaga gccaagacta
     1561 tccaggatag ccttggtgtg ttgatcaggt gtatccaaaa aagtaactaa tgcgattgcc
     1621 atggtattgt ccttatcagg ctaatttaaa cagttgttcg atcatccgtt ccttaacaat
     1681 aagaaccgaa catcgggcgt tcatcatgac agatcgatac gtagaggaca taatatcttt
     1741 ggaatcgacc gtttctcctt cctgcccccc aaggagtatc agatccattt ttcgctcttc
     1801 tgacatcgtc acaacctcgg accaaattgc accgcggcgt aactctgttt caatcttgac
     1861 ccctttagcc ttacctaatt cttctgcata cgaaaggtaa cgacggccgt tttcttcaag
     1921 actctgctca tattcgcggc tttcctcatc aataaaaatt cgattcac
//
LOCUS       DAOQXQ010000350         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_68062_length_1963_cov_2.262055, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000350 DAOQXQ010000000
VERSION     DAOQXQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68062_length_1963_cov_2.262055"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKH81_06225"
     CDS             <1..242
                     /locus_tag="PKH81_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNQ97667.1"
                     /translation="LEELSTQVDGILVIGGKNSANTTRLFRMAESLCSGRAWHIEDER
                     DIPAAAYDLGRIGLTAGASTPDEVIDAVEQALQNG"
     gene            293..>1963
                     /locus_tag="PKH81_06230"
     CDS             293..>1963
                     /locus_tag="PKH81_06230"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ97668.1"
                     /translation="MKAIELEKAYNPGDFEDRVYAAWESNGCFKPRQDSTQTVYGERP
                     FVVVIPPPNVTGVLHMGHGLNNSLQDIVVRFHRMRGEPTLWVPGTDHAGIATQNVVER
                     RLKKEGKSRHDLGRDAFVKETWKVKNEHHAIITNQLKKIGASVDWSRERFTLDDGCSR
                     AVREVFVTLYERGLVYRGNYLVNWCTSCGTALADDEVEHQDTAGAMHHIWYELEEGVT
                     AADGATRIEIATTRPETLLGDTAVAVHPEDPRYASLVGKQVHLPLTGRMIPIIADEYV
                     DREFGTGMVKITPAHDPNDWEVSKRHALPVINILNPDGTLNNSVPEKYRGMKCPEARK
                     AVKADLEELGLYKSEEKITHAVGKCYRCSTVVEPYLSDQWFVRMKPLADKALDAWKKG
                     EIRFYPQKWENIYAHWMENIRDWCISRQLWWGHRIPVWYCADCGKMTVSRDDPSACSS
                     CGSTRITQDPDVLDTWFSSWLWPFSTLGWPDKTPDLASFYPTTALVTAYDIIFFWVSR
                     MIMAGLEFTGQVPFRDIYIHGLVRDKQGRKMSKSLGNGIDPLEIVSEYG"
BASE COUNT          460 a          493 c          515 g          495 t
ORIGIN      
        1 ccctggaaga gctttccacc caggttgacg gcatcctggt catcggcggt aaaaactccg
       61 caaatactac tcgtctgttt cgtatggctg agtccctttg ttctggaagg gcatggcata
      121 tcgaggatga acgagatatt ccggcagcgg cctatgatct cggtcgcatc ggcttaaccg
      181 ccggcgcttc cactccagac gaggtgattg acgctgttga acaagccctg caaaatggat
      241 aatatcgttt tgtcaggctt acccacaccc atatacaagg aattatcgat acatgaaggc
      301 tattgaactc gaaaaagcgt acaatccagg ggactttgaa gatcgtgtct atgctgcctg
      361 ggaatcaaac ggatgtttta agccccgtca ggattctact cagacagtgt atggggagcg
      421 cccctttgtt gtcgttattc cgcctcccaa cgttactggc gttcttcata tgggtcatgg
      481 cttaaataac tccctgcagg acattgtggt ccgatttcat cgcatgcgcg gtgaacctac
      541 cttgtgggtt cccggtaccg atcacgcagg tattgccact caaaacgtgg tggagcgtcg
      601 gcttaaaaaa gaagggaaaa gccgtcatga tcttggccgg gatgcctttg ttaaagaaac
      661 ctggaaagta aaaaacgaac accatgccat tattaccaat cagcttaaaa aaatcggggc
      721 gagcgttgac tggagccggg aacgttttac cctggacgat ggatgttccc gcgcggttcg
      781 cgaggtcttt gttactctat acgaaagagg cctggtgtat aggggtaatt acctggttaa
      841 ctggtgtacc tcctgcggaa ccgcccttgc ggatgacgaa gttgagcatc aggataccgc
      901 cggcgcgatg catcatatat ggtatgagct tgaggaaggc gtaaccgcgg ccgatggcgc
      961 cactcgcatc gaaatcgcaa ccacgcgtcc agagacactc ctgggtgata ctgcggttgc
     1021 tgttcatccc gaagatcccc gctatgcttc tcttgttggc aaacaggttc acttaccgct
     1081 taccggccga atgataccga taatcgccga tgaatacgtt gatcgcgagt ttggaaccgg
     1141 tatggtcaag attactcctg cccacgatcc aaatgactgg gaggtttcca agcgacatgc
     1201 cctgccggtt atcaatatct tgaatccaga cggaaccctg aataattcgg ttccggagaa
     1261 ataccgcggc atgaagtgcc ccgaggcccg aaaagcggtt aaggcggatc ttgaagaact
     1321 tggtctttat aagagcgagg agaaaattac ccatgcggtt ggaaagtgtt atcgctgttc
     1381 aaccgtggtt gagccctatc tttccgatca atggttcgtt cgcatgaagc cgcttgccga
     1441 caaggccctg gatgcctgga aaaagggaga gattcggttt tatccacaaa agtgggaaaa
     1501 catctatgcg cactggatgg aaaatatccg cgattggtgt atctcccggc agctgtggtg
     1561 gggccatcgt attccggtat ggtattgcgc cgactgcggc aaaatgacgg tatcgcgcga
     1621 tgatccctcg gcctgctctt catgcggatc aaccaggatc actcaggacc ctgatgtgct
     1681 tgatacctgg ttctccagct ggctctggcc cttttctacc cttggttggc cggacaagac
     1741 tcctgacctt gcaagctttt atccaaccac cgccctcgtt accgcctacg atattatttt
     1801 cttttgggtc tctcgtatga taatggctgg ccttgagttt actggccagg ttcccttccg
     1861 cgatatctat attcatgggc ttgttcgcga caagcagggc cgcaagatgt ctaaatccct
     1921 gggtaatggc attgatcctc tggagattgt cagtgaatac ggt
//
LOCUS       DAOQXQ010000351         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_68064_length_1963_cov_2.232704, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000351 DAOQXQ010000000
VERSION     DAOQXQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68064_length_1963_cov_2.232704"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKH81_06235"
     CDS             complement(<1..1434)
                     /locus_tag="PKH81_06235"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ97669.1"
                     /translation="MADTTIERPVFPKKAVVTGGMPYGNKKLHLGHIGGVFIPADIFT
                     RFLRDRIGSENVVFVSGTDCYGSPIVEYHRRAVAEGKFSGSIQDFVRHNHNAQKAELE
                     SYYVSNNLFAASALDRAGEIHRELSAQILSTLHANGWLKKMSTPQFFDPKMNVFLNGR
                     QVIGRCPIPGCKSEKAYADECELGHPYEPRDLIAPKSVLTGEAPQMKDVTNWYIDLPS
                     FSKEFAQWIAALEENPGVRPFITSTIKEFLAPPVIYVKQDFEAELQLVRPALPEHTLR
                     EDPSKALPLEFKTLKERDTACAILTSKGIRFRAGKTLTPFRLTGNVDWSIKAPVLEGV
                     EGLTFWVWPESLWAPVSFTKAYLEQEGKDSSEWKKYWCSPDAKVYQFIGEDNIYFYSL
                     AQQAIFMGLQGASPQVFPPEGQLQPTELIANKHLLQFKKKASSSGDIPPIMASDLLKH
                     YTPDQLRAHFCALGLSLRSINFSPKPFN"
     gene            1563..>1963
                     /locus_tag="PKH81_06240"
     CDS             1563..>1963
                     /locus_tag="PKH81_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97670.1"
                     /translation="MLHRYKLIILAFVLLAPSVPALSMEVIRHTYSSQEPDAQFEEIL
                     YLTIAVHLTTRGYSSTKTSENAPYILLTEYTVRGSEADLVFSLLDAGLIDSERARYSV
                     TVQIDYTLEGALDQAIDELLAEARLDEESTDV"
BASE COUNT          515 a          478 c          472 g          498 t
ORIGIN      
        1 attaaagggc ttgggactaa aattgatcga acgaaggctt aatccaagcg cacaaaaatg
       61 ggcgcgaagc tgatccgggg tgtaatgctt aagtaaatcg cttgccatga ttggtggaat
      121 atccccggaa gaagaggcct tcttcttgaa ctgcaaaaga tgcttgttcg caattaactc
      181 tgttggctga agctgtcctt cgggtggaaa tacctgtgga gaggcgccct gcaggcccat
      241 gaatatagct tgctgggcaa ggctgtaaaa gtaaatgtta tcctcaccta taaactgata
      301 cactttggcg tcgggtgagc accagtattt tttccattcg gaactgtctt ttccctcttg
      361 ctcaagatat gccttggtaa aggaaacagg tgcccataag gactcgggcc acacccaaaa
      421 agttaatccc tctacccctt ccaacacggg agccttgata ctccaatcca cattacctgt
      481 caggcgaaac ggggtcaggg ttttaccggc cctaaaacga attcctttgc tggtcaagat
      541 tgcgcatgcg gtatcccgct ccttaagcgt tttaaactca agcggcaagg ccttacttgg
      601 atcctcgcgc agagtatgtt caggaagagc ggggcgaacc agttgaagtt ccgcttcaaa
      661 atcctgttta acatagatta ccggaggggc aagaaattct ttaatggtag aggtaataaa
      721 ggggcgcaca ccaggatttt cttccagtgc cgcaatccat tgggcaaatt ctttagagaa
      781 tgacggaaga tcaatatacc agttggttac atctttcatc tggggcgctt cgccggtaag
      841 cacgctcttt ggcgcgataa ggtctcttgg ctcatacgga tggccaagtt cacattcgtc
      901 ggcatacgcc ttctccgact tacagccggg tattggacag cggccgataa cctggcgacc
      961 gtttaaaaag acgttcatct taggatcaaa gaactgcggg gtactcattt tcttgagcca
     1021 accattagca tggagggtag aaagaatctg ggcgcttaat tcgcggtgaa tctcaccggc
     1081 ccgatccagg gcagaggccg caaaaagatt attagataca taatagcttt ccagctcggc
     1141 tttttgggca ttatgattgt gacggacaaa atcttgaata gaaccggaga attttccctc
     1201 ggcaacggca cgacgatggt attcaacaat cggagatcca tagcagtcgg tccccgacac
     1261 aaagacaacg ttttcgcttc cgatacgatc gcgtaaaaac cgggtaaaaa tatcggcggg
     1321 gataaacacg cccccaatat ggccaagatg cagttttttg ttgccatagg gcataccacc
     1381 ggttactact gcttttttcg ggaaaacggg tcgctctatc gttgtatccg ccataatact
     1441 ctccaatacg gtctggtaat cattgtagtc agtacgattt tactgaaaaa cacaggccta
     1501 aacaaggctc aaggcaggga taaccaggtg cccaaataag agaaaagccg tatactttag
     1561 atatgcttca tcgttataag ctcatcatcc tggcttttgt gctactggca ccgtcggttc
     1621 ctgcactatc catggaggtt attcgccata cctattcctc ccaggagcca gatgcccagt
     1681 ttgaggaaat tctctattta accatcgcgg tacacctgac tacacgggga tattccagta
     1741 ccaagacctc tgaaaacgcc ccctacatcc ttcttaccga atacacagtc cggggaagcg
     1801 aagccgatct tgttttttcg cttcttgatg cgggtctcat cgattcagag cgtgcacgct
     1861 attctgttac cgtacagatt gactacaccc tggagggggc cctggatcag gcgattgatg
     1921 agcttcttgc tgaagcccgc cttgatgaag agagcactga tgt
//
LOCUS       DAOQXQ010000352         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_68356_length_1958_cov_2.148187, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000352 DAOQXQ010000000
VERSION     DAOQXQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68356_length_1958_cov_2.148187"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="PKH81_06245"
     CDS             <1..1516
                     /locus_tag="PKH81_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97671.1"
                     /translation="KSSGISFCMAEMEKAYIKTMTARYPNVPAEEFLTRDPVKAFDYL
                     ERYFVLNIPKDLRIVLIIDYAETIVPADEIGHLDEIDRYCLVTLNRWSHEPQFTRGDV
                     SVIMLTENLTDINPRLSTSPSTVKVTIPFPDQLVRTQFLEFLDSKEMLILERGLNPER
                     IGVLTSGLNLLNLNQLVAESYQEDKPITLDYLRLRKRAIIENEAAGLLEFVETEHDLS
                     FVGGHGFVKKRFQSAAKALKQGRIDVLPMGYLIAGPVGTGKTFMVSAFAGEIGIPMVK
                     LRNFRSKWQGATESNLEKVLNILKAMSPVAVMIDEADAFLGDRDAQGDSGTSNRVFAQ
                     IANFMGNTEYRGKIIWFLITCRPDLLPIDLKRQGRAEEHLALFYPETVDEKVELFRTL
                     QRKLDIKVKDFPLADIFKSFKFEVSGSDIEAILVRSKMNATMDGHTIVTREDIDQTIR
                     DFIPPAYPHEIELQNLVAVLECTSREMVPARYQKLERSKLAGEIQELKQLLGEA"
     gene            complement(1517..>1958)
                     /locus_tag="PKH81_06250"
     CDS             complement(1517..>1958)
                     /locus_tag="PKH81_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97672.1"
                     /translation="LIASESSLRAAITYSANEQLLLQEYLANSHGRDVRILLAGDTLV
                     AAYERVSAQGNFKSNACEGAIAHKASWEGIDYWIQMARDICARAGLWYASVDFLFRSP
                     PSQKMVELAVCEVNASPGFEALDRDLCMDSATILIDAMIQDFFS"
BASE COUNT          504 a          470 c          494 g          490 t
ORIGIN      
        1 caaatcgagc ggaatatcct tttgtatggc agagatggaa aaagcataca taaagactat
       61 gaccgcccgc tatcccaatg tcccagcaga ggaatttttg acacgggacc cggtaaaggc
      121 ctttgattat ctggagcgct actttgtgtt aaacatcccc aaggatcttc ggatcgtatt
      181 gattatcgac tacgccgaaa cgattgttcc ggcagacgag ataggacacc ttgacgaaat
      241 agaccgctat tgcctggtaa cccttaatcg ctggtcgcac gaaccgcagt ttacccgggg
      301 cgatgtctcg gttatcatgc ttaccgagaa tctgaccgat atcaatcccc gcctgtcgac
      361 ttctccatca acggtaaagg taaccattcc ctttcctgac caactggttc gcacccagtt
      421 tttggaattt ttagatagca aagagatgtt gattctggag agaggtctta atccggaacg
      481 tattggcgtc cttacctccg gtttgaacct tcttaatcta aatcaactgg tggcggaatc
      541 ctatcaggag gataagccca taaccctgga ttatttacgg cttcgcaagc gggcaatcat
      601 cgaaaacgag gctgcagggc ttcttgagtt tgtggaaacc gaacatgatt tatcgtttgt
      661 tggcggacat ggttttgtaa aaaaaagatt ccaaagtgcg gcaaaggccc taaaacaggg
      721 acgaatagat gtgttgccca tgggctattt aattgcgggg ccggttggaa cggggaaaac
      781 ctttatggtt tctgcctttg ccggcgaaat cgggattccc atggttaagc ttcgcaattt
      841 ccgctcaaaa tggcagggtg ctaccgaaag caatctggaa aaggtgctta acatactaaa
      901 ggcgatgagc ccggttgcgg ttatgataga tgaggccgac gcctttcttg gcgatcggga
      961 cgcacagggg gactcgggaa cctcaaaccg tgtgtttgcc cagattgcaa actttatggg
     1021 gaataccgag tatcggggaa agattatctg gttcctgata acctgtcgcc ctgatctttt
     1081 acctatagat ttaaagcgtc agggccgtgc cgaggagcat cttgccctgt tttatcccga
     1141 gactgtcgac gaaaaggtag aactttttcg tacacttcag cgtaagctcg atatcaaggt
     1201 aaaggatttc ccccttgccg atatttttaa gtcgttcaag tttgaggtat ccggctctga
     1261 tattgaggcg atactggttc gctccaagat gaatgccacg atggacggac atacaatcgt
     1321 tacccgggag gacatcgatc agacaatcag ggactttatc cctccggcat atccacatga
     1381 aattgagctt cagaatttgg ttgccgttct tgagtgtacc agccgcgaaa tggttcccgc
     1441 tcgctatcaa aaactggagc ggtccaaact tgccggtgaa atacaggaac tgaagcagct
     1501 attaggggaa gcgtgattag gaaaaaaaat cctgaatcat cgcatctatc agtatagttg
     1561 cgctatccat acaaagatcc cggtccaggg cttcaaatcc cggggaggcg tttacctcgc
     1621 atacggctag ctctaccatc ttttgggagg gtggcgacct gaacagaaag tcgacgcttg
     1681 cataccagag ccctgcccgg gcacagatat cacgagccat ctgaatccag taatcgatac
     1741 cttcccatga cgccttgtgt gcaatcgctc cttcgcacgc gttcgactta aagttgccct
     1801 gcgctgatac ccgctcgtaa gcggcgacaa gggtatctcc cgcaagaaga atacgcacgt
     1861 ctcttccatg ggagttggcc aagtattcct gcagcaacag ctgctcatta gcgctatagg
     1921 taatggcggc acggagggat gattctgagg ctatcagg
//
LOCUS       DAOQXQ010000353         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_68466_length_1956_cov_2.927407, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000353 DAOQXQ010000000
VERSION     DAOQXQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68466_length_1956_cov_2.927407"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKH81_06255"
     CDS             complement(<1..1088)
                     /locus_tag="PKH81_06255"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNQ97673.1"
                     /translation="MITLTNTSSGKKDVSLVSFVEWCLYNALDDTANFQRNFSTGEVE
                     VDGSAIYHKTEYRERRNHFAFYSVNAKLDGFDTDRETFLGLHNNYDAPDTVMAGKSQN
                     FVADGWSPIASHRLNVSLEPGATKTYIFVLGYVEMEQDKKWAGENGCAPASKDLLQTN
                     PKMRVINKEKALALQSRFQTVDQVNAAFADLKKYWDGLLSNFTLKTGDEKLDRMAVWN
                     QYQCIVTYNFARSASYFESGIGRGIGFRDTSQDMLGVVHQIPSRARERLFDVASTQFE
                     DGSAYHQFQPLTKRGNADIGSHFNDDPLWLVLGVGGYIRETGDVDFLNEMVPFDNSPT
                     KKASMFEHLKRSFHYTVTHLGPHGLPLIG"
     gene            complement(1085..1285)
                     /locus_tag="PKH81_06260"
     CDS             complement(1085..1285)
                     /locus_tag="PKH81_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97674.1"
                     /translation="MDNNGRYFYIKDGDAVWNPGWKPMRVELDSYECRHGFGYSKLSS
                     SKNGLKADVLYMVPTEKMPRCR"
     gene            complement(1312..1476)
                     /locus_tag="PKH81_06265"
     CDS             complement(1312..1476)
                     /locus_tag="PKH81_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97675.1"
                     /translation="MKFGHFDDGNREYVIETPRTPYPWINYLGNTQFFSLISNTAGGY
                     ASIPTLACAD"
     gene            complement(1575..>1956)
                     /gene="nagB"
                     /locus_tag="PKH81_06270"
     CDS             complement(1575..>1956)
                     /gene="nagB"
                     /locus_tag="PKH81_06270"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665046.1"
                     /note="catalyzes the reversible formation of fructose
                     6-phosphate from glucosamine 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNQ97676.1"
                     /translation="ADGHIAFNEPGSSLTSRTRVKTLTQDTIIMNSRFFGGDPERVPT
                     RALTVGVGTIADAEEVMILASGYTKARALRHALESGVNHMWTISVLQMHPKAIVVCDE
                     DATDELKVGTVRYFKDIEAKNNNP"
BASE COUNT          499 a          478 c          482 g          497 t
ORIGIN      
        1 ccgataagcg gaagcccatg gggcccaagg tgggtaacgg tgtaatggaa gcttcgttta
       61 agatgctcga acatcgacgc cttcttggta gggctattgt caaagggaac catctcgtta
      121 aggaagtcta cgtctccagt ctcacggatg tatccaccta caccaagcac cagccacagc
      181 gggtcatcgt taaagtgact gccaatgtcg gcattaccgc gcttggttag cggctggaat
      241 tgatgatagg ccgaaccgtc ttcaaactgg gtagaggcaa catcaaagag cctctcccgg
      301 gcacgggaag gaatctgatg aacaacacca agcatgtcct ggcttgtatc gcggaacccg
      361 attccgcgtc caataccgct ttcaaagtaa gaggctgaac gggcaaagtt ataggttacg
      421 atacactgat attgattcca taccgccatg cgatcaagct tctcgtcgcc agtcttaagg
      481 gtaaagttgg aaagaagacc atcccagtat ttctttaaat ccgcaaaggc tgcgttaacc
      541 tgatcaacgg tttggaaacg ggattgaagg gcgagcgctt tttccttatt gattacccgc
      601 atcttgggat tggtttgaag gaggtccttg gaagcgggag cacacccatt ctctccagcc
      661 catttcttat cctgttccat ctccacatag ccaagcacaa agatgtatgt ctttgttgcc
      721 cctggctcaa ggcttacgtt aagccgatgg ctcgcaatgg gagaccatcc gtcagcgacg
      781 aaattctggc tcttaccggc cataacggtg tcgggagcgt cgtagttatt gtgaaggcca
      841 aggaaggttt cgcggtcggt atcaaagccg tccaacttgg cgttaaccga ataaaaggca
      901 aagtgattgc gtcgttcacg atattcggtt ttatgataaa tcgcgcttcc atcaacctct
      961 acctctccgg tagaaaaatt gcgctggaag tttgcggtat catcaagggc gttatacaaa
     1021 caccattcca caaaggaaac gagggagacg tcttttttac cggaggaggt gttggtcaag
     1081 gtaatcatct gcacctcggc attttctccg ttggaaccat atacaacaca tctgccttaa
     1141 ggccgttctt ggaagaagaa agctttgaat atccaaaacc atggcggcat tcataggaat
     1201 caagctcgac ccgcatgggc ttccatcccg ggttccatac cgcatcgcca tctttgatat
     1261 agaagtaacg gccgttattg tccagcggta catcgttata gcgataacgg gtcagtcggc
     1321 gcaggcgagc gtcggtatag aggcgtaacc gcctgcggta ttggaaatca acgagaaaaa
     1381 ctgtgtgttt ccaaggtagt tgatccaggg atacggagtc cgtggcgtct cgataacata
     1441 ctcacggttt ccgtcgtcaa aatgaccaaa tttcatatcg tctccttatc aggttgattg
     1501 atttgttcaa ccaacttaag tgtatcatag tccctgcccc ctgtcaaagg gcagaggctt
     1561 tttttatgga gatattaggg attgttgttt ttagcttcaa tatccttaaa atatcgcacg
     1621 gtaccaacct tcaattcgtc ggttgcgtcc tcatcgcaca caactattgc cttggggtgc
     1681 atttgcaata cagagattgt ccacatgtga ttaaccccgc tctcgagggc atgcctaaga
     1741 gctcgtgcct tggtgtatcc agaagccaga atcattacct cttcggcatc ggcaattgtg
     1801 ccaacaccaa cggttaaggc ccgagtcgga acccgttcgg gatctccgcc aaaaaaccgt
     1861 gaattcataa tgatcgtatc ctgagtaagg gtttttaccc gagttcgtga ggtaagagag
     1921 gatcccggtt cattaaacgc gatgtgccca tcagcg
//
LOCUS       DAOQXQ010000354         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_68784_length_1950_cov_4.026913, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000354 DAOQXQ010000000
VERSION     DAOQXQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68784_length_1950_cov_4.026913"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKH81_06275"
     CDS             complement(<1..471)
                     /locus_tag="PKH81_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97677.1"
                     /translation="MTNDEHPFKRFVLCLCRITCGFLLLACSSGSNDDDPDSTKYDIT
                     IEVIGAEGNDEIIVNTQTAKEGDTIKVSARYSESNRRVDLSAEGITFYPPSITVETAH
                     FVMPAAPVVIKATFTDILYTVTVIPEGAIGSECVTANVTKAKEGTLVTLSVSIGA"
     gene            558..1826
                     /locus_tag="PKH81_06280"
     CDS             558..1826
                     /locus_tag="PKH81_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ97678.1"
                     /translation="MKKVYISIYEYACERRELDAQKIHNFLSASSYSIVSKPQLADII
                     IFITCGIIEKNTEACLQYIKKYQKYEAQLIVGGCGPGIDKERIQAIHKGAMFTTSDMR
                     ALDSLFPDQAVPFSSIDDAPFKFSMLDKGIFGSIATKWFFAIPRVKPLFKKIVRLVLN
                     TRFGPYSFVSLKAVGLDNEYYIKIADGCVSQCSYCALSNAIGPLKSKPMASCLAEFKR
                     GLEQGYTNFYLTGDDTGAYGIDCGHTLPDVLEEITSVQGTYNLLISSFNSAWLCTYID
                     RIESLVTNGRIKSMVIPVQSGNSEVLTAMNRFSDVAKIKNAVTRIKKACPQIWLSTHI
                     IVGFPSESDSAFQDTLTFATTTPFDEVQLFAFSLKPGTKAQDITPRISEATIAARMNK
                     ARDFFKKNKYTCYMHKTDFLYASQYRAPRL"
BASE COUNT          590 a          445 c          412 g          503 t
ORIGIN      
        1 cgccccgata gaaacggaca aggttaccaa ggttccctcc ttagccttgg taacattagc
       61 cgttacacat tccgaaccaa tagcgccttc cggaatcacg gtaacagtat ataaaatgtc
      121 agtgaacgta gctttaataa cgaccggagc ggcgggcata acaaagtgag cggtttctac
      181 agtaatagaa ggaggataaa acgtaatccc ctctgcactc agatcaactc gacgattact
      241 ttcagagtac ctggcggata ccttgatcgt atctccttct ttggccgtct gtgtattaac
      301 gattatctca tcatttcctt cggcgccgat aacctcgatg gtgatgtcat actttgttga
      361 atcagggtca tcatcgtttg aacctgatga acacgcaagg agcaaaaacc cgcaagtgat
      421 gcgacacaga cataacacaa atcttttgaa aggatgttca tcattagtca ttgtacaagc
      481 gtataggtaa tccggcaaga cttgaaatct gtacgagtct ttggaggtat gatattatca
      541 tttccaagga atagcgtttg aaaaaagtat atatcagcat ctatgagtat gcctgcgagc
      601 gaagagagct tgatgcccaa aagattcata atttcttgtc ggcttcctca tatagtattg
      661 ttagtaaacc tcagttagca gacattatta tttttatcac ctgtggcata atcgaaaaaa
      721 acaccgaggc ctgtttgcaa tacatcaaga aatatcaaaa gtatgaggcg caactaatag
      781 tcggaggttg tgggcccggc atagataaag agcgcatcca ggcgatacac aagggcgcca
      841 tgtttactac cagcgatatg agagcactcg attcgttatt ccccgaccaa gccgttccgt
      901 tttctagtat cgatgacgcc cctttcaaat tttcaatgct cgacaaaggg atatttggca
      961 gcatcgcaac aaaatggttt tttgccatcc cccgcgtcaa accgcttttt aagaaaatag
     1021 ttcgattggt attgaacacg cggtttggac catactcgtt tgtttcatta aaagcagtgg
     1081 gacttgataa cgaatattac atcaaaatcg ccgatggctg cgtaagccag tgtagttact
     1141 gcgccctgtc gaatgctatt ggccccttaa aaagcaaacc catggcaagc tgccttgcag
     1201 agttcaaaag aggtttggaa cagggctaca ccaattttta tcttacgggc gacgacaccg
     1261 gcgcatatgg cattgattgt ggacacaccc tgccagatgt attggaagaa ataacctcag
     1321 tacaaggaac atacaacctt ttgatcagtt catttaattc tgcctggctt tgtacttaca
     1381 tagatagaat agaatcgctc gttaccaacg gaaggattaa aagcatggtc attcccgtgc
     1441 agtcaggaaa ttctgaggtg cttacggcca tgaaccgctt ttccgacgta gcaaaaatta
     1501 agaatgcggt aacgcgcata aaaaaagcct gcccacagat ttggcttagt acacacataa
     1561 tagtcggctt cccctcagaa agcgacagtg cctttcaaga caccctgacc tttgcaacaa
     1621 caacgccctt tgacgaagtc caactattcg ccttcagttt aaaaccgggg acaaaggccc
     1681 aagacattac tccccgaatt tccgaagcta ctattgccgc cagaatgaac aaggcccgag
     1741 atttttttaa gaaaaacaag tatacctgtt acatgcataa aactgatttt ctgtatgctt
     1801 cacaatacag ggctccacgg ttataagata atgagcgacg acgtttatat tggaatcgat
     1861 attggatcta ccactatcaa ggtggcagta ctaaacgcgg acaaccattt gctggaaaca
     1921 cgctatgagc ggcacaacct ggcaataaag
//
LOCUS       DAOQXQ010000355         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69177_length_1944_cov_2.200106, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000355 DAOQXQ010000000
VERSION     DAOQXQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69177_length_1944_cov_2.200106"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKH81_06285"
     CDS             complement(<1..436)
                     /locus_tag="PKH81_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97679.1"
                     /translation="MQPYARALGFAEPTILFPERIVDAWHAFNRKETRMIIGFRHAYG
                     DDPQMTAYTIHHALPKAARKIGKPLKGLTHTHFIYGAEVPLWSGPFVRWLLPEAGAIP
                     VDHVRMDSSGMALIRRLMIEGAFPIALAPEGHVTHDSETVGDL"
     gene            663..1700
                     /gene="asnA"
                     /locus_tag="PKH81_06290"
     CDS             663..1700
                     /gene="asnA"
                     /locus_tag="PKH81_06290"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNQ97680.1"
                     /translation="MPQLTLPAGYKALLGVKETEQAIIRIKEFFQLALSTELNLTRVT
                     APLFVPRGKGLNDDLNGIERPVHFPVKDLNEEELEIVHSLAKWKRVKVSDLALPPGFG
                     IYTDMNAIRADEELDNIHSLYVDQWDWELAMEEQDRTIDFLKDIVRKIYRCLKRTEFF
                     VYDNYPVITPSLPQEITFVHAEDLYHQWPTLTAKERENEAAKKWGAVFIIGIGYALAD
                     GNLHDGRAPDYDDWITETGNGKRGLNGDLLVWNSVLGQSLELSSMGVRVSAASMTEQL
                     AYRNCPERASLYFHSRLLSGSLVQTAGGGIGQSRLCMFFLRKAHIGETQVSVWPQSMI
                     ADCSKAGIPLL"
     gene            1697..>1944
                     /locus_tag="PKH81_06295"
     CDS             1697..>1944
                     /locus_tag="PKH81_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97681.1"
                     /translation="MKIPIVLISLQVQSSFQAVPLGAASIVSALKNDRFITDRCTVSL
                     CDLSLEDPQFHDKDDECIARIIADRAFDCACLSAGCDT"
BASE COUNT          477 a          436 c          478 g          553 t
ORIGIN      
        1 ctagatctcc taccgtttcg ctgtcatgcg ttacatgacc ttctggagcc agggcaatcg
       61 ggaatgcacc ctctatcatt aaacgacgta tcagagccat gccagagcta tccattctaa
      121 catgatctac cggaatggct cccgcctctg gcagtagcca acgcacgaat ggtcctgacc
      181 atagaggaac ctctgctcca taaataaagt gtgtatgggt caagccttta aggggtttac
      241 caatttttct tgctgccttt ggaagggcat gatggatggt atacgccgtc atctgtgggt
      301 catcgccata ggcatggcgg aaaccgataa tcattcgggt ttctttccgg ttaaaagcat
      361 gccaggcatc tactatacgt tcagggaaaa ggattgtggg ttctgcaaaa ccaagggcac
      421 gtgcataggg ctgcattatc gatcggaaaa agtaaataac gagtttgttt agtttgggtt
      481 ctgctttcgg tctttcttgt agtggtgtag taacgggagc gtacatgtaa tccagtctac
      541 ggcctgcctg aaaaaaaggc aagagtcaag atgctgagta cgccttttca tctggacggt
      601 gtagcccctg atacggtatt atgtttttta ttacccgttg cgaacggaca aagaggtcac
      661 ctatgccaca gctcacgctt cctgccggat ataaggctct tttgggagtt aaagaaacag
      721 aacaggctat tatcagaatc aaagaatttt ttcagcttgc actatctaca gaattaaacc
      781 tgacgcgggt taccgcacca ttgtttgtcc ctcgcggcaa gggacttaac gacgatctta
      841 atggaataga gcggccggta catttccccg taaaggatct taacgaagaa gaactggaga
      901 tcgttcattc tctggcgaag tggaagcggg ttaaggtttc ggatttggct cttcctcccg
      961 gttttggtat ttataccgac atgaacgcta tccgggcgga tgaggaactg gacaatattc
     1021 actctctgta tgttgatcag tgggactggg aactggcaat ggaagagcaa gatcgaacta
     1081 tcgattttct aaaggatatc gtcaggaaga tataccgctg tcttaagcgt accgagtttt
     1141 tcgtgtatga caattatcct gtaataactc cgtctctacc gcaggaaatt acctttgttc
     1201 atgccgagga tttatatcac cagtggccaa cgctcactgc aaaagagagg gagaacgaag
     1261 ccgccaagaa atggggcgca gtatttatca ttggtatcgg ctatgcactt gcggacggta
     1321 atcttcacga tggtcgagcc ccggattacg atgactggat taccgaaacg gggaatggaa
     1381 agcgcggtct caatggtgat ctccttgtat ggaattctgt gcttggacaa tcccttgaac
     1441 tttcttccat gggggttcgt gtaagtgctg cttccatgac agagcaactt gcttatcgca
     1501 attgtcctga acgagcgtct ctgtattttc attctcgcct tctttcgggt tcactcgttc
     1561 aaacagctgg cggtggtatc ggccaatcac gtctttgtat gttctttttg cgtaaagcgc
     1621 acattggtga aacacaggta tctgtatggc cccaatccat gatcgctgat tgttcaaaag
     1681 cgggaatccc ccttctttga aaattccgat agtacttatt tccctgcagg ttcagtcatc
     1741 ctttcaagcg gttccattag gagccgcttc gattgtaagc gcgcttaaaa acgatcgctt
     1801 tattactgac cgatgcacgg tttcattgtg cgatctttct ttggaggatc ctcagttcca
     1861 cgataaagat gatgagtgta ttgcgcggat tatcgcagat cgtgcctttg actgcgcttg
     1921 tctttctgca ggttgtgata ctca
//
LOCUS       DAOQXQ010000356         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69263_length_1942_cov_3.411765, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000356 DAOQXQ010000000
VERSION     DAOQXQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69263_length_1942_cov_3.411765"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PKH81_06300"
     CDS             <1..1014
                     /locus_tag="PKH81_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNQ97682.1"
                     /translation="GLERALTDTIQLIKGSFALAVMTEKTLIGARDPNGIRPLCLGKL
                     KNGWVLASESCAIDAMGGDLVRDIEPGEIVIINDDGVLSFNFGEKTAKKTCIFEYVYF
                     ARPDSIMDGIGVLEARIKMGEVLARESGVTADVVIGVPDSGLGAAMGYSRASGIPYAM
                     GIVKNKYIGRTFIAPSQEEREQMVFVKLNALKSDLDGKRVVIIDDSIVRGTTSRRLIQ
                     ILRKAGAKEVHFRISSPPVKYPCYFGIDTPARAELISATHEIEEIRQAIGADSLAFIS
                     MDGMIEALQSCEWEEETTKEKKPSRAANDKNGDSGASNCGYCQGCFLGEYPMPMIGEL
                     GKR"
     gene            1057..>1942
                     /gene="purM"
                     /locus_tag="PKH81_06305"
     CDS             1057..>1942
                     /gene="purM"
                     /locus_tag="PKH81_06305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNQ97683.1"
                     /translation="MIDYRQSGVDVEEGYRAVDNYKSSAKRTMIPGVLNGLGSFAGMF
                     QIPAGYKEPVIVSGTDGVGTKLDIAFALKKYDTVGIDCVAMCVNDILCHGATPLFFLD
                     YLACGKLDAHIAGDMVKGVAEGCLQAGAALMGGETAEMPGFYDEGKYDIAGFAVGIAE
                     KSEIIDGSKIREGDVLVGLSSSGVHSNGFSLVRKLITDYSEPFNGKPIGETLLEPTKI
                     YVKPILALMKQVDIHGMVHITGGGFYENIPRMYPAGGPDGAGDAQTRLVSKIQKGSWT
                     IPPIFNELVRRGANPDRMF"
BASE COUNT          519 a          450 c          528 g          445 t
ORIGIN      
        1 ggacttgagc gagcgcttac cgacaccatc cagctcataa aaggttcatt cgccctggcg
       61 gtaatgaccg aaaaaaccct tatcggggcg cgagacccta acggtatcag accgctgtgt
      121 ctgggcaagc ttaaaaacgg atgggttctt gccagcgaat cctgcgcaat cgatgcgatg
      181 ggcggggatc tggtgaggga tattgaaccg ggagagattg ttatcatcaa tgatgacggg
      241 gtgctttcgt tcaactttgg agaaaagaca gcaaaaaaaa catgcatctt tgaatatgta
      301 tactttgccc gtccggactc catcatggac ggtattggtg tattggaagc tcgcattaaa
      361 atgggagagg tcctggctcg cgaatccggg gttaccgcag atgttgttat tggcgtgccg
      421 gactcgggtc ttggggccgc aatggggtat tcccgagctt ccgggattcc ctacgcaatg
      481 ggcattgtta aaaacaagta cataggcagg acctttatcg ctcccagtca ggaagagcga
      541 gagcaaatgg tgtttgttaa gctcaatgcc cttaaaagcg atctggacgg caaacgagtg
      601 gttattatcg atgattcaat cgtacgtgga acaaccagcc gtcgccttat ccagatattg
      661 cgcaaggcag gagctaaaga ggttcacttc aggatttcct cccctccggt aaaatatccc
      721 tgctactttg gtatcgatac ccccgcacga gctgagctca tttctgcaac ccacgagata
      781 gaagaaatcc gtcaagcgat tggcgcggat tcccttgcct ttatctctat ggacggcatg
      841 atagaagccc tgcagtcctg tgaatgggaa gaagaaacca caaaagagaa aaaaccgtcc
      901 cgtgccgcca atgataaaaa cggagattcg ggcgccagca attgtggata ctgccaaggg
      961 tgcttcctgg gtgaatatcc aatgccgatg atcggggaac tgggtaagcg ctaagcacaa
     1021 taaaaacgca tataacacga taacaaggag actgtaatga tagattaccg ccaatcaggc
     1081 gtggacgttg aggaaggata tcgggcggtt gataattaca agagcagcgc aaaacgcact
     1141 atgatcccgg gcgtattaaa cggtctgggc agttttgccg gaatgttcca gattcccgcc
     1201 ggatacaaag agccagttat cgtttccgga actgacggcg ttggtaccaa attggatatt
     1261 gcctttgccc ttaaaaaata cgatacagtg ggaatagact gtgttgccat gtgtgttaac
     1321 gacattcttt gccatggggc cacgcccttg ttttttctgg actaccttgc atgtggcaaa
     1381 ctagatgccc acatagccgg agacatggta aagggtgttg ccgaaggatg tctgcaagca
     1441 ggggccgccc tgatgggagg cgaaaccgcc gagatgcccg gtttttatga tgaggggaaa
     1501 tatgatatcg cgggttttgc cgtgggtatc gcagaaaagt cagagattat cgacggctcc
     1561 aaaatccggg aaggagatgt cctggtgggt ctttcttcga gcggtgttca ctcaaacggc
     1621 tttagcctgg ttcggaaact aatcactgac tacagcgaac cctttaacgg aaagcctatc
     1681 ggggagaccc ttcttgagcc gaccaagata tatgttaagc cgattttagc gcttatgaag
     1741 caagtagaca tccacggcat ggttcacata accggcggcg gattttacga gaacatcccg
     1801 cgcatgtacc ccgctggggg acctgatggc gcaggcgatg cacaaacaag gctcgtctcc
     1861 aagatacaaa aaggcagttg gaccattcct cctatcttta acgaactggt tcgcagagga
     1921 gcaaatcccg acagaatgtt ca
//
LOCUS       DAOQXQ010000357         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69736_length_1934_cov_3.227249, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000357 DAOQXQ010000000
VERSION     DAOQXQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69736_length_1934_cov_3.227249"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..354
                     /locus_tag="PKH81_06310"
     CDS             88..354
                     /locus_tag="PKH81_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNQ97684.1"
                     /translation="MTTKNVTIRNRAGIHARPAALIAQTANRFASEILIERDGTRINA
                     KSIMGIITMAASYNSVLTLCADGTDEKEAIEALDSLFINKFEED"
     gene            351..1454
                     /locus_tag="PKH81_06315"
     CDS             351..1454
                     /locus_tag="PKH81_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97685.1"
                     /translation="MKLIRRSIPSVLFSAPFLFFILVLIALASFLLANSGFEGLLSTH
                     LFTSPQGLLVLVIGPFALFVLLGLLLYSVVSHALHTVQATRFGHKLFVTMAVLIACAS
                     IPSGIIVSRFATTVIGSWFDREVSRSLSYAAEMADLYTAERIRDIEKVASRFFNGLAI
                     ANNRNRPADWMSDIRLIDAYAASCQVYAIEGDGFLPVVEYGDSSLFYPRSALDRVRDG
                     FFVWQEDESVFRYGKTVHYSNSVYVCVYTSLIPSSFHDKSQLIQDARIKGDVIRILEP
                     FLPFLGVWVYFLFCFPSVVMTIILAYYLSVGVSAPVASAAEAATRLEQGTPSLRPIVT
                     GNDEVAGLCNSLNRLCDAADGSKKKDKKAVIRL"
     gene            1479..>1934
                     /locus_tag="PKH81_06320"
     CDS             1479..>1934
                     /locus_tag="PKH81_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNQ97686.1"
                     /translation="MADTAPVWLFTGPEIGERNTAIDDIRQKAVKVLGDLDYHSLYAS
                     DTRMGEVISLLQNGSLFSGGRFILLRNAEEIKKKEDVSMVVDWITSAASITDAYLVLV
                     SEDIGIDRKIEAAIPQGHKRVFWEMFEDRKEQWIVNYFRTSGFAIDPDAV"
BASE COUNT          439 a          447 c          485 g          563 t
ORIGIN      
        1 aaccaaaacg tacttaaatg gattgaaact gatgctgaac gcgcgccgta ttatggcgtt
       61 gacgattcct attaaaggat agacaggatg acaacaaaga acgttacaat acgcaatcgc
      121 gccggaattc acgcgcgtcc tgcagccctc atcgcccaaa cggcgaaccg atttgcctca
      181 gagattctga tagaacgcga cggaacccgt attaacgcaa agtcgattat gggtattatc
      241 acaatggcgg ccagctataa cagtgtattg accctctgtg ccgatggaac ggacgaaaaa
      301 gaggcaattg aagctttgga ttcgctgttc atcaataaat tcgaggaaga ttgaaactga
      361 ttcgacgaag tatcccttcg gtcctctttt ctgctccctt tttgttcttc atcttggtcc
      421 ttatagccct tgcctccttt cttttggcaa actcaggctt tgagggcctt ctgtcaacac
      481 acttgtttac ctctccccag ggcttgctgg tgttggtcat cggtccgttt gctctgtttg
      541 tgctcttagg attgttgtta tatagcgtgg tatcccatgc tcttcatacc gtccaagcca
      601 cacgttttgg acacaagctc tttgtaacaa tggctgtgtt aattgcctgc gcctctattc
      661 cctcaggcat tatcgtcagc cggtttgcga ctacggttat tggttcatgg tttgaccggg
      721 aagtgtcccg ctccctttca tatgcggccg aaatggctga tttatataca gccgagcgaa
      781 ttcgagatat cgagaaggtt gcttcgcgct tctttaacgg ccttgccatc gcgaacaatc
      841 gcaatcgccc cgccgactgg atgagtgata ttcggcttat cgatgcctat gcggcaagtt
      901 gccaggtata tgcaattgaa ggagacgggt ttttgccggt ggtagagtat ggggattcat
      961 ccttgtttta tccccgttct gccctcgatc gtgtcaggga cggttttttt gtatggcaag
     1021 aggatgaatc ggtgtttcgc tatggtaaaa ctgtccatta cagtaacagt gtgtatgtgt
     1081 gtgtctatac atccctgatc ccttcttctt ttcacgataa atctcagttg attcaagatg
     1141 ctcgtatcaa gggcgacgtt atccggatac tcgaaccctt tttgccattt ttaggggtgt
     1201 gggtgtattt tttgttttgt tttccctcgg tagttatgac catcattttg gcctattacc
     1261 tcagtgtcgg ggtgagcgcc ccggtcgctt cggctgcgga ggctgctact cgtcttgagc
     1321 aaggcactcc ttcgctccgg cctatcgtaa cgggaaatga tgaggttgcg ggcctttgta
     1381 attcacttaa tcgcctctgc gacgctgcag acggttcgaa aaaaaaggac aaaaaggcgg
     1441 taattcggct ataattgccg gcaagtgagg aacttctgat ggcagacacc gctccggtat
     1501 ggctattcac cggacccgaa attggcgaaa gaaacaccgc gatagatgac atccgtcaaa
     1561 aagcagtaaa ggtcctgggc gatcttgatt atcattcgtt gtacgcctcg gatacccgaa
     1621 tgggtgaggt gatatccctt ttacaaaatg gctctctttt ttcaggcggc cgttttatcc
     1681 tgctcaggaa cgccgaagaa atcaagaaaa aagaggatgt atccatggtt gtggattgga
     1741 ttacgtccgc tgcctcgatt accgatgcat atctggtatt ggtttcagag gatattggta
     1801 ttgaccggaa gatcgaagct gccattcccc agggacataa gcgggtgttt tgggagatgt
     1861 tcgaggatcg aaaggaacag tggattgtca attactttcg tacttccggg tttgctatcg
     1921 atcctgatgc ggtc
//
LOCUS       DAOQXQ010000358         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69844_length_1932_cov_3.725093, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000358 DAOQXQ010000000
VERSION     DAOQXQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69844_length_1932_cov_3.725093"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKH81_06325"
     CDS             <1..427
                     /locus_tag="PKH81_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97687.1"
                     /translation="RTILATLKDTISAIRLATEQNDEVISFFSRVSEDTQSVSLMIEE
                     LIYSIKEVSSSSTDVLQAVQVVSELTGKTEETVQESLKNLSMCSNGIVQVSSAAAQVL
                     RDTQEMATRYDSIIIDAQKAQALGVENLETITALKQELG"
     gene            490..768
                     /locus_tag="PKH81_06330"
     CDS             490..768
                     /locus_tag="PKH81_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ97688.1"
                     /translation="MNKAELIDVIADDAGLTKKDAGAALKAFTDAVAKELKKGGSVQL
                     VGFGTFEVGKRAARSGRNPKTGETIKIKAAKTPKFKAGKALKDIVNKK"
     gene            complement(845..1393)
                     /locus_tag="PKH81_06335"
     CDS             complement(845..1393)
                     /locus_tag="PKH81_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97689.1"
                     /translation="MKKAVLGIGALSLLFLCTVNLFAIDISAGGGVSVNVLSHAETST
                     EYDESRVLIGGGAHIFADATYLLATAGISLMEDFTYADLNLLAKYPFTFSSFTVFPLA
                     GGSYFVNFDDTHSNAWGIKGGLGLDYAVSEGLYIRPMALVGYRFHSFRDDTLRALFKL
                     YGYDLSITKITVDVSVALGYRL"
     gene            complement(1408..>1932)
                     /locus_tag="PKH81_06340"
     CDS             complement(1408..>1932)
                     /locus_tag="PKH81_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97690.1"
                     /translation="GKRARLTIAKVDPRAIEHGPRRAIKEVLMQLVRNSMTHGIEKPE
                     ERLARGKEEEGCITLTIDMVKDRIEIKLSDDGNGLDFDAIRKKAVELSLVGDEAELQD
                     KSSVLKILFAPGFSTTEKADMHSGRGVGLNLVKERVKELKGTIKLQSEDGKGTVFIIS
                     IPPEQDALTGVQTA"
BASE COUNT          586 a          475 c          399 g          472 t
ORIGIN      
        1 ccgaacaatc ttggcgacgc ttaaagacac aatatcggcc atccgtcttg ccaccgagca
       61 aaacgacgag gtaatctctt tcttttcaag ggtttcggaa gatactcaaa gcgtatcctt
      121 gatgatcgaa gagcttatct atagcattaa agaagtatcg tctagttcca ccgatgtttt
      181 gcaagctgtt caggttgtgt ccgaactaac cggaaaaaca gaagaaaccg tacaagagtc
      241 gcttaaaaac ctgagtatgt gtagcaatgg cattgtgcaa gtttcttcgg ccgcagcaca
      301 ggttttacga gacacccagg aaatggccac tcgctatgac tccataatca ttgatgctca
      361 aaaagcccaa gctcttggtg tagaaaacct ggaaacaatc accgcattaa aacaagagct
      421 tggataaagg cctatggtat ggtatactct accagataca gtattgaatt tcccaaggag
      481 aaaaccatta tgaacaaagc ggaattgatt gacgtaatcg cggacgatgc cggtctaacc
      541 aagaaggacg ccggagccgc gctcaaggca tttactgatg cagttgccaa agaactaaag
      601 aagggtggat cggtccagct cgttggcttt ggaacctttg aagttggtaa acgcgcagcc
      661 cgttcaggca gaaaccccaa gactggcgaa acaatcaaga tcaaagcggc aaagacaccc
      721 aagttcaaag ccggaaaagc gcttaaagat atcgtcaaca agaaataagc agacaacgct
      781 ttagtgcata aaaaaagcct gtcaggaaaa ccctgtcagg ctttttttta tacgtgccag
      841 tatcctacag tcgatacccc agcgcaacag acacatccac cgttatcttg gttatggaaa
      901 gatcatagcc atacagttta aagagcgccc gcaacgtatc atcgcgaaaa gaatgaaacc
      961 gatagccaac caaggccatg ggtctaatat acaaaccttc tgatacagca taatcaagac
     1021 cgagcccgcc ttttatgccc caagcgtttg agtgtgtgtc gtcaaagttt acaaagtagg
     1081 atccgcccgc aagaggaaat actgtaaacg aagaaaacgt aaagggatat tttgcaagca
     1141 ggttcagatc agcataggtg aagtcttcca taagcgaaat acccgctgtt gccaaaaggt
     1201 atgtcgcgtc agcaaatata tgcgcaccgc caccgataag caccctactc tcgtcatact
     1261 cggtagaggt ttccgcatga gaaagtacat tcacggatac gcccccgcct gcgcttatat
     1321 caatggcaaa aagattaaca gtacacaaaa acaacagaga gagcgcacct atccccaata
     1381 cagctttctt cattctacat ccttttctta cgcagtctgt acgccggtaa gggcgtcttg
     1441 ttccggagga atggaaataa taaatacagt tccctttcca tcttcagact gcaattttat
     1501 ggtaccctta agctctttaa cacgctcttt aacaagatta agacccacac ccctgccgga
     1561 atgcatatct gctttttctg ttgtgctaaa gcccggcgca aaaagtattt ttaaaacgga
     1621 ggacttgtcc tgaagctctg cttcatctcc aaccaaagaa agctccaccg ctttttttcg
     1681 tatcgcgtca aaatcaagac cattaccatc gtcggaaagt ttgatctcaa ttctatcctt
     1741 taccatatca atagtaagag tgatgcatcc ttcttcttcc ttacctcgtg caagtctctc
     1801 ttcaggcttc tctatgccat gcgtcatgga attacgaacc agctgcatca atacttcttt
     1861 aatagcgcgc cttgggccat gttctatagc tcttggatct acttttgcaa tcgtaaggcg
     1921 ggcacgcttg cc
//
LOCUS       DAOQXQ010000359         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69935_length_1931_cov_2.854478, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000359 DAOQXQ010000000
VERSION     DAOQXQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69935_length_1931_cov_2.854478"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="pgi"
                     /locus_tag="PKH81_06345"
     CDS             <1..882
                     /gene="pgi"
                     /locus_tag="PKH81_06345"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189392.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ97691.1"
                     /translation="GLEPSRHMIAVTSETSPLANNPGYLASFYMDDFIGGRYSTSSAV
                     GGAILSLAFGSAAFEAFLDGAHQADKAALESDIRKNAALLDALIGVYERNVLGYPSTA
                     VLPYSQALSRFPAHLQQADMESNGKRVNRQGEPVDYATGPVIFGESGTNGQHSFYQLL
                     HQGTDIIPLQFIGFIDSQSGKDIVVDGSTSQKKLCANLAAQIVAFACGKKDANPNKHF
                     PGERPSSLIWGKTLDPKALGALLAHFENKIMFQGFIWNLNSFDQEGVQLGKVLTKKVL
                     SGDMDDTLKAYAGIFGL"
     gene            complement(897..1019)
                     /locus_tag="PKH81_06350"
     CDS             complement(897..1019)
                     /locus_tag="PKH81_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97692.1"
                     /translation="MKTNAMRILVSAGVRGTQIILAPDDLVRITDALVCDISTQ"
     gene            complement(1026..>1931)
                     /locus_tag="PKH81_06355"
     CDS             complement(1026..>1931)
                     /locus_tag="PKH81_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNQ97693.1"
                     /translation="VFFCFNALFLCYTVDMTSTFEAFLSPHVNRPSFISGWLTAHSVP
                     NTQVLLSGRTHVVVRFANTAYDPRFRMKTLVAHHDRAPLTPGANDNSAACFQLMLFAW
                     RLSRLTTIHNVRILFTDGEEAAGVSGIAAQGSYTVGSALRSRGEDIGDVYVLDATGRG
                     DTLVVSTTALDSIKNRQLQRGMDILHQKTRQIARKTAQESWVSLPTPYSDNAGFLATG
                     IPAQVLTILPATEATILLHAQSEYTTLDVLTKPALDDDSRLYYPETWKLMHTPNDGAH
                     TLTPTAFSLMRIFLDNLARTMEGIE"
BASE COUNT          465 a          473 c          514 g          479 t
ORIGIN      
        1 ggtcttgaac cctcccgcca catgatcgcg gtaacaagcg aaaccagtcc ccttgccaac
       61 aatcctggat atcttgcctc tttttacatg gacgatttta ttggcggacg atattccact
      121 tcctccgcag ttggcggagc gatcctttcc ctggcctttg gaagtgctgc gtttgaagcc
      181 tttcttgatg gcgctcatca ggcggacaag gcagcactcg aaagcgatat tcgcaaaaat
      241 gcggccttgc ttgatgccct tatcggggta tacgagcgca atgtgcttgg ctatccttct
      301 acggccgttc ttccctatag ccaggccctg agccgtttcc ccgcgcatct tcagcaagcg
      361 gatatggaat ctaatggtaa gcgggtaaat cgtcagggag agcccgttga ttatgcgacc
      421 ggtccggtca tttttggaga aagcggtaca aacggacaac attcgtttta tcaattactt
      481 catcaaggaa ctgatatcat tccgctccag tttattggct ttattgacag tcagtcaggc
      541 aaggatattg tcgttgacgg ttcaacaagc caaaagaaac tctgcgcaaa ccttgctgca
      601 cagattgtag cctttgcctg tggaaaaaag gatgctaatc caaataaaca cttccccggg
      661 gaacgccctt ccagcttgat ctggggaaag acccttgatc caaaggccct tggagccttg
      721 ctggcccatt ttgagaataa aatcatgttc cagggcttta tctggaattt gaacagcttt
      781 gatcaggaag gcgtacagct tggaaaggtc ttaaccaaga aggtactctc aggcgatatg
      841 gacgacaccc ttaaggcata cgcaggaatc tttggcctgt aaggcgcttg taccgcttac
      901 tgggtgctaa tatcgcagac tagtgcatcc gtaatgcgta ccaggtcatc gggggccagg
      961 ataatctgtg tcccccgcac accggcgctt accagaatcc gcatcgcgtt cgttttcata
     1021 ctgtcttatt ctattccttc catggtacga gccaggttgt ccaaaaaaat tctcatcagg
     1081 ctaaatgccg tgggagtaag ggtgtgagcc ccatcgttcg gggtatgcat gagcttccag
     1141 gtttcaggat agtaaagtct tgaatcatcg tccagggcgg gttttgtcaa cacatccagg
     1201 gtggtatatt cgctttgcgc atgcaagagg atagtcgctt ccgtcgcagg taatatggtc
     1261 agtacctggg ctggaattcc tgtagccaaa aatcctgcgt tatcgctata cggggtaggc
     1321 agggataccc aactttcctg agcggtttta cgcgctatct gtcgcgtttt ttgatgcagg
     1381 atgtccattc ctctttgaag ctggcggttc ttgattgagt caagggccgt tgtggaaacg
     1441 acaagggtat ctccccgtcc ggtggcatcc agaacataga cgtctccaat gtcttcccca
     1501 cgcgagcgca aggcgctccc tacggtatag gatccctggg cggcgatgcc cgaaactcct
     1561 gccgcttctt ctccgtcggt aaaaaggatg cgtacgttat gtattgtcgt gagtcgggac
     1621 agtctccagg caaagagcat gagctggaag caggcagcgc tgttgtcatt agctcccggc
     1681 gtcaggggcg cccggtcatg gtgagcaacc agggtcttca ttctgaagcg gggatcgtag
     1741 gcggtattgg cgaaacggac gacaacatgt gtgcgtccgg aaagaagcac ctgagtgttt
     1801 ggcactgagt gcgcagtgag ccagccggaa ataaaagacg gacgattaac gtggggggat
     1861 aaaaaagctt cgaaggtaga agtcatatcg acagtatagc aaagaaacag ggcattgaag
     1921 caaaaaaaaa c
//
LOCUS       DAOQXQ010000360         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_69995_length_1930_cov_3.140800, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000360 DAOQXQ010000000
VERSION     DAOQXQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69995_length_1930_cov_3.140800"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1141)
                     /locus_tag="PKH81_06360"
     CDS             complement(170..1141)
                     /locus_tag="PKH81_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97694.1"
                     /translation="MRKYLVLLFLVTLPLYAEQVQSMDNIARDFFANQYEDPIIEATA
                     EEQPSAFSGLRYYRVYERKNQVEDPLIPDEWIRTKDNQFYRMNELRAVLTKEGIIPST
                     TKEALSYARELLVRTTKTNCIIDDDMVSAGYIPPRYATRPYYPAVEELPGGYRVTLYL
                     YYLPRRFAQFFSIYHKNVVEYIITNQGSEYRITTGRVYPPAPSGIEEDLTQMIVALLR
                     EKDFIQSLEPYIQKKTIYGISDPSDLEEVNRRNAELGKLWKQGFTQSFPEAAALTRRV
                     LAKSTSFTFDEEYIWEETRESKPAKQTEHYRETLRILLSDIVQRERE"
     gene            complement(1154..>1930)
                     /locus_tag="PKH81_06365"
     CDS             complement(1154..>1930)
                     /locus_tag="PKH81_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97695.1"
                     /translation="KMTLQASSAIEEMTASIGNITAITTKNRDSVESLARSISGGIES
                     AEESSATITSLIDSGTSLQDVVEVITAISSQTNLLAMNAAIEAAHAGDAGKGFAVVAE
                     EIRHLAEETAVNSKTIADGLGNLFRQISNAETANKTMGGAFKDIADEVKKTEMAFAEI
                     LAGMSDLSAGTKDINVAVSDFVTASREVTESIRNINEMIANNTTSIDGIRDKSARTLQ
                     GIEEITSNFTEILSRSATVQELGRQSDGVFKELDLSIRSM"
BASE COUNT          423 a          448 c          467 g          592 t
ORIGIN      
        1 attcctgaaa tagtcgataa tagcgaataa gcagggctgc gtaatgaacc cggtttggac
       61 ttgccaatgg cgagaaaaag gtttgaggca ccgtagcgaa tattccgctg gatctgatac
      121 tcatgcttta ttatagaaga ataaaggtag ttttggccat aaatttacct tattcccttt
      181 cccgctgcac tatatccgaa agcagtatac gcaaggtttc tcgatagtgc tctgtctgtt
      241 ttgccggctt actttccctc gtttcttccc agatatattc ttcgtcaaag gtaaacgatg
      301 tagatttcgc gagcactctg cgggtaagag cggccgcttc cggaaaagat tgggtaaagc
      361 cctgtttcca aagcttaccc aattcagcgt tgcgtcgatt cacttcctca aggtcgctcg
      421 ggtcggatat tccgtagatc gtcttttttt ggatgtaggg ttcaagactt tggataaaat
      481 ctttctcacg gagtagtgca acgatcattt gtgtaagatc ttcttctatc ccgcttggtg
      541 cggggggata cacccgacct gtggtaatgc gatactcgct cccctggttg gttatgatat
      601 attcaactac gttcttatga tatatcgaga aaaactgggc aaaccgcctg ggaagataat
      661 acagataaag agtaactcga taaccacccg ggagctcttc cacggctgga tagtatggtc
      721 ttgtggcgta tcggggagga atatatcctg cgctgaccat gtcgtcgtca ataatgcaat
      781 tggtcttggt ggtacggact aaaagttcgc gcgcatacga tagcgcttct tttgttgtcg
      841 aaggaataat gccctctttg gttagcactg ctcgtagctc gttcatccgg taaaactggt
      901 tgtcttttgt tctaatccat tcatcgggta tgagggggtc ttctacctgg ttttttcgct
      961 cgtatacgcg gtaatatcgg aggcctgaga atgctgacgg ctgttcttcc gctgttgctt
     1021 ctatgattgg atcttcatac tggttggcga aaaaatcacg ggcgatgttg tccatggatt
     1081 gtacctgttc ggcgtaaagg ggaagggtaa caagaaaaag taagaccaga tattttctca
     1141 tcacccgctt ccattacatt gacctgatgg aaaggtcaag ctccttgaat accccatcgc
     1201 tttgtcggcc cagttcttga accgtagccg agcgggagag tatctctgta aaatttgagg
     1261 tgatttcttc tatgccctgc aacgtgcgtg cgcttttatc gcggatgccg tctattgaag
     1321 tagtgttatt cgcaatcatt tcgttaatat tgcgaatgga ttcggttact tcccgcgatg
     1381 cagtgacgaa gtcggacacc gctacgttaa tgtccttggt tcccgccgaa agatcgctca
     1441 ttcccgcaag tatttctgca aaggccatct ctgttttctt aacttcgtcg gctatgtcct
     1501 taaaggcgcc gcccatggtt ttatttgctg tttctgcgtt gctgatttgt cggaacagat
     1561 ttcccagtcc atcggcaatg gtttttgagt taaccgcagt ttcttcggca agatggcgta
     1621 tctcttctgc aacaacggca aagccctttc ccgcatctcc tgcgtgcgct gcctctattg
     1681 ctgcgttcat ggctaacaga tttgtctgac ttgatatcgc cgtgattact tcaacaacat
     1741 cctgaagcga ggttccgcta tctatgagcg aggtgatcgt ggccgatgat tcttctgcac
     1801 tttcgattcc gcccgagatg cttcgcgcaa gcgattctac gctgtccctg ttttttgtgg
     1861 tgatggcagt tatgtttccg atcgacgcag tcatctcctc gattgccgat gatgcctgca
     1921 gggtcatctt
//
LOCUS       DAOQXQ010000361         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_70308_length_1925_cov_3.600000, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000361 DAOQXQ010000000
VERSION     DAOQXQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70308_length_1925_cov_3.600000"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1052)
                     /locus_tag="PKH81_06370"
     CDS             complement(60..1052)
                     /locus_tag="PKH81_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNQ97696.1"
                     /translation="MTSYKDLGLVNTKEMFAKAIKGGYAIPAYNFNNMEQMQAIIQAC
                     VETKSPVILQVSAGARKYANKNLLKNMAKGAVEYAHELGCDIPIVLHLDHGDSLELCV
                     DCIESGFSSVMIDGSHLPYDENVALTKKVCEYAHKHGVTVEGELGVLAGVEDDVSSEV
                     SHYTKPEEVQDFVSKTGVDSLAISIGTSHGRQKFKPEQCTKNADGVLIPPPLAFDILA
                     EIEKKLPGFPIVLHGSSSVPVEYVKEIEQYGGKLSDSVGIPEEQLRKAAKSAVCKINI
                     DSDGRLAMTAAIRKVLAEKPDEFDPRKYLGPARDELKKLYMHKNTNVLGSAGQA"
     gene            1216..>1925
                     /locus_tag="PKH81_06375"
     CDS             1216..>1925
                     /locus_tag="PKH81_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNQ97697.1"
                     /translation="MKQGGLIIAGLVLLFSLCVLPVAAQTRSVNYETYIVDNFDDPDA
                     SEWTWIAAGSKFATKGYPILKYFDGMPNAIRVMQTNADTGYKFLGMEVKFDRKGDNWV
                     DIVPTKKGTGDKVEPYEIPFKGRIARLDMWVWGANYAYELEVLVRDCNGRVHSVPVGL
                     VKHEGWRNMSVTIPTNIQQASPYLSGIQQMSFVAFRLRTRPTERVDAFYIFFDQFKAL
                     TDTYLPSYDGFELVGAEFT"
BASE COUNT          481 a          417 c          488 g          539 t
ORIGIN      
        1 cttaaaaagt atcgtgcata aaaaaaaccg cccgaaggcg gtttttctcg ttcagggtat
       61 tacgcctgtc cagcagaacc aagaacgttc gtgttcttgt gcatatagag ctttttaagc
      121 tcgtcacgag ccgggccaag gtatttgcgg ggatcgaatt catccggttt ctccgcaaga
      181 accttgcgaa ttgcagcggt catcgcaaga cgtccgtctg agtcaatatt gatcttgcaa
      241 acagcgcttt ttgcagcctt gcgcagttgt tcctcgggga taccaaccga gtcggaaagt
      301 tttccgccgt attgttcgat ttccttaaca tattcaaccg gaactgagga cgaaccatga
      361 agaacaatcg ggaagccggg gagtttcttt tcgatttcgg caaggatatc aaaggcgagt
      421 ggagggggaa tgagaacgcc gtctgcattc ttggtgcact gctctggctt gaacttctgg
      481 cgtccatggc ttgttccgat ggaaatggcg agagagtcaa ccccggtttt agaaacaaag
      541 tcctgtactt cttcgggctt ggtgtagtgg ctaacttcgg atgaaacgtc gtcttcaaca
      601 ccggcaagaa cgccaagttc accttctacg gttacaccat gcttatgtgc atattcgcaa
      661 acctttttgg ttaaggcaac gttttcatcg tagggcaagt gggatccatc gatcatgaca
      721 gatgaaaagc cgctttcaat acagtctaca cagagctcaa ggctgtcgcc atgatccagg
      781 tggagtacaa tggggatatc acagccgagt tcgtgggcat attctacagc acccttggcc
      841 atgttcttga gcaggttttt gttggcatac tttcgagctc ctgcagaaac ctgaagaatt
      901 accggggatt tggtttctac acacgcctga atgatggcct gcatctgttc catgttgtta
      961 aagttatacg caggaatagc gtagccaccc ttgatggcct tggcaaacat ttcctttgtg
     1021 tttacgagac cgagatcttt atagctggtc atctgacact cctctatcga taaaagatac
     1081 tccaatttta gagattcccc tgtgtttggt caatacaaac agaataaacg gtttgacaaa
     1141 gggttttctt caccatataa taataatact tgactgtatt ctgtccgatg gtatagttgt
     1201 taaaaggagt ttgggatgaa acaaggcggt ttaatcatcg cgggccttgt ccttctgttc
     1261 tccctgtgtg tgttgccggt agcggcccag accaggtcgg tgaactatga gacgtacatc
     1321 gtggataact tcgatgatcc cgatgcgtcc gaatggacat ggattgctgc aggaagcaag
     1381 tttgctacta aagggtaccc gatcctgaag tactttgatg gaatgcccaa cgcgatcagg
     1441 gttatgcaga ccaatgccga cacgggatac aagttcctgg gtatggaggt caagtttgac
     1501 cgtaagggtg acaattgggt ggatatcgtt ccgaccaaaa aaggtaccgg agataaggta
     1561 gagccgtatg aaataccctt taaaggacgg atagcccgtc ttgatatgtg ggtatggggc
     1621 gctaattatg cgtatgaact tgaagtgctt gttcgggatt gtaatggaag agttcattct
     1681 gttccagttg gtttggtaaa gcatgaaggt tggagaaata tgtcggttac aattccgaca
     1741 aatattcaac aagcatcgcc gtatctgagc ggtattcaac agatgtcttt tgttgcgttc
     1801 aggttgcgca ctcgtccaac agaacgcgtt gacgcatttt acatcttttt tgatcagttc
     1861 aaggcgctta ccgacaccta tctgccctct tatgacggat ttgagcttgt tggcgctgag
     1921 tttac
//
LOCUS       DAOQXQ010000362         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71039_length_1913_cov_2.749193, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000362 DAOQXQ010000000
VERSION     DAOQXQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71039_length_1913_cov_2.749193"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKH81_06380"
     CDS             <1..1057
                     /locus_tag="PKH81_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ97698.1"
                     /translation="EDKVVRFAERERHQLFLEPEGLDTDELYINGLSSSLPEEVQDEF
                     MRTVPGLAEAKVTRPSYAVEYDYIDPTQLLPSLETKRVRGLFTAGQINGTSGYEEAGG
                     QGLIAGINAALYVRALQEGSLSGPGGVSVPAHLRPFVLGRADAYIGVLIDDLVTLGTK
                     EPYRMFTARAEYRLRLRHDTADERLTAMAREIGLQNDEAMERLQQRLSKREELTRLME
                     GRRIGSSDIGLRPSLTPHLGKSSADALRDPLVPLEDILSLDDSFTAFSPEIATSAELV
                     IRYAHYIDAQDRRVDRLKRMDGKVIPNDFDYDSVSGFSMESRTKLKNVRPQTLGQAAR
                     IPGLRNSDVMLLMVHLK"
     gene            complement(1061..1912)
                     /locus_tag="PKH81_06385"
     CDS             complement(1061..1912)
                     /locus_tag="PKH81_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNQ97699.1"
                     /translation="MNLGMRYSSYLLWSGLPIPDFPRKMNGLDDMHDIITKYTRPRCK
                     TGDDVISLIDMSIRDAIADGVTVLEGSIDIGFVVHFKKDLDKFLEMVSDFTEKYRDKI
                     DIRPELGMGKTFDKSKIAKWAPILLDSKIFRSIDLYGPEVEDGIEEFKSIFAQAERLG
                     LKKRAHVGEFSDATSVRRFVEYFDLHEVQHGIGAASDESVLRFLADRKIRCNVCPQSN
                     VILGAVPSLKAHPVKKMLNAGVPVTIGTDDLLFFGSTVAEQIFDLLSNDVITEAEAEI
                     LVCEGVQ"
BASE COUNT          446 a          446 c          521 g          500 t
ORIGIN      
        1 agaagacaag gtagttcgtt ttgccgaacg cgagcgccat caattgtttc ttgagccgga
       61 aggacttgat actgacgagc tgtatataaa cggcctttcc tcctcgcttc ccgaagaagt
      121 tcaggatgag tttatgcgaa cggttcccgg tcttgccgag gccaaggtaa cgcggccctc
      181 ctatgcggtt gaatatgatt atattgatcc aactcagctt ttgccttctc ttgaaacaaa
      241 acgggtgaga gggcttttta ccgcgggcca aataaatgga acctccgggt acgaggaagc
      301 tggcgggcaa ggattgattg cgggtattaa tgccgcccta tatgtgcgtg ccctgcagga
      361 aggctccctt tccggacctg gaggggtgtc tgttcctgcg catctgcggc cctttgtatt
      421 ggggcgcgcc gacgcttaca tcggtgtatt gattgatgat ttggttactc tgggaacaaa
      481 ggagccatac cgaatgttta ccgctcgggc ggaatatcgg ttacgactcc gccacgatac
      541 cgctgatgag cgattgaccg caatggcacg cgagattggt ttacaaaatg atgaggcaat
      601 ggaaaggctt cagcagcgtc tttccaagcg tgaggagctc actcgcctga tggagggacg
      661 cagaataggt tcatccgata tcggccttcg tccgtccctg acccctcact tgggaaaatc
      721 gagtgccgac gcgcttcggg atcccctggt tcccttggag gatattcttt ccctggatga
      781 ttcttttacg gccttttctc ccgagattgc cacatcggct gagctggtaa ttcggtatgc
      841 ccattatatt gacgctcagg acagacgggt ggacaggctt aagcgtatgg atggcaaggt
      901 gataccgaac gattttgatt atgattcggt gagtggtttt tctatggaat cgcggaccaa
      961 gctaaaaaac gtacggccac agacgttggg ccaggcagcg agaattcccg gtttgcgtaa
     1021 ttcggatgtg atgttgttaa tggttcacct gaaataggtg ttactgaacg ccttcgcaga
     1081 cgaggatttc ggcctctgct tcggtaatga cgtcgttgga tagaaggtca aatatttgtt
     1141 ctgccacggt tgaaccaaag aataaaaggt cgtcggtacc aatagtgacg ggaactccgg
     1201 cgttgagcat ctttttaacc gggtgtgcct ttagcgaggg caccgctccc aggataacat
     1261 tgctttgcgg gcagacattg cagcggattt tgcggtcggc caggaagcga aggacggact
     1321 cgtcgctggc cgcgccgatt ccatgttgca cctcgtgaag gtcaaagtat tctacaaatc
     1381 gccgcactga ggtagcgtcg gaaaactccc caacatgtgc acgtttttta agaccaaggc
     1441 gttcggcttg ggcgaatatt gatttgaatt cttcgatacc gtcttctacc tcgggtccat
     1501 agagatcgat acttctgaaa attttactgt ctaaaagaat tggcgcccat tttgcaatct
     1561 tggacttgtc aaaggttttg cccattccca attcgggccg gatgtctatt ttatcgcggt
     1621 atttttcggt aaagtcgctg accatttcca ggaatttgtc caagtccttt ttaaaatgaa
     1681 caacaaaacc aatatcgatg gagccttcca gcacggtaac tccgtcggca atcgcatctc
     1741 gaatggacat atcgataagg ctgataacat catcgccggt tttacagcgg ggacgggtat
     1801 atttggtaat aatgtcgtgc atgtcgtcca ggccgttcat tttgcgcgga aagtcgggaa
     1861 tcgggaggcc tgaccataaa aggtacgaag aatagcgcat gccaaggttc agg
//
LOCUS       DAOQXQ010000363         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71145_length_1911_cov_2.804418, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000363 DAOQXQ010000000
VERSION     DAOQXQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71145_length_1911_cov_2.804418"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKH81_06390"
     CDS             complement(<1..657)
                     /locus_tag="PKH81_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNQ97700.1"
                     /translation="MEHLGAWGLIPPLLTIALAFITKDVIISLFLGIVSGLLIVAGGN
                     PALALINTTDMLAAALNDGWNIRIFLFCALLGGLVGMLSRTGSSSALGVWAARHLKTG
                     RSSQLAAWFFGLLIFIDDYFNSLTVGTMMRPITDAHRVSRAKLAYILDSTAAPVCIIA
                     PISSWVVTVMSVVKGSDRFESLGISEFEFFIRSIPYNLYALLTLAMVVVIAVTKRDFG
                     P"
     gene            774..>1911
                     /locus_tag="PKH81_06395"
     CDS             774..>1911
                     /locus_tag="PKH81_06395"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNQ97701.1"
                     /translation="MKTKICLVLNAPTIRENLALVELYRQWIDIAELRVDCLAQEERL
                     LFRRFPEQAGIPCILTIRRKSDGGQFVEGEGSRTAMLARGLAFADINPHKNFAYIDLE
                     EDLQVPSIEEAARAFGTRIIRSVHNMEGPETDIRARVQSLRRTKDEIAKIAFTPRNLS
                     DVTKLFKDAKCCQSEEYILIAMGQYGLPSRILSPLLGSSVCFVSSEESIRTSGNPLGQ
                     LDPVTLNEVYRFREISASTRVFGITGNPLAATSSPAIHNKGYRVQGLDAVYIPIKAEN
                     AEEAIEFAEEVGITGLSVTFPFKESVIPNLSEISANTGEIGACNTIVKSARGWIGYNT
                     DEAGFSRALLQFLGKKDLKRVRVSIIGAGGAARAITHAIKQLGAK"
BASE COUNT          531 a          516 c          471 g          393 t
ORIGIN      
        1 gggcccaaag tcccgtttcg ttaccgcgat taccacaacc atagccaggg ttaacagggc
       61 atacaagtta taaggaattg acctgataaa aaactcgaac tcgctgatgc caagactctc
      121 aaaacggtca gagcccttta ctacggacat taccgtaaca acccagctgg aaatgggcgc
      181 gataatacac accggagccg ctgtagagtc aagaatatac gcaagctttg cccgcgacac
      241 ccggtgcgcg tcggtaattg gccgcatcat ggtcccaacg gtaagagaat taaaatagtc
      301 gtctataaaa ataagcaaac caaaaaacca tgcggcaagc tgggagcttc tgcctgtttt
      361 aagatgccgc gccgcccaca cgccaagggc gcttgacgag cccgtgcgcg atagcatgcc
      421 gacaagaccg cccaaaaggg cacaaaacaa aaagatacgg atgttccatc cgtcattaag
      481 ggcagcggca agcatgtccg tggtgttaat cagggccagt gcaggatttc ctccagcaac
      541 gatcaagagc cctgatacaa tccccaggaa aagagagata ataacatcct tggttataaa
      601 ggccagggca atcgttaaca ggggtggtat aagcccccaa gcgcctaagt gttccattgg
      661 ttcgtactcc ttattagggt cattgtaaac gtagttaggg caagcctcaa ttaaaaaaaa
      721 agcgatttct cctcttccta ctccgccttt gcgctggtat actttgcccc aagatgaaaa
      781 ccaagatatg cctggtgctt aacgccccga ctatcaggga aaacctcgcc ctggtggaac
      841 tgtaccgcca atggatagat atagccgaac tacgcgtaga ctgccttgca caggaagaac
      901 gccttttgtt tcgtcgcttt ccggaacagg cgggaattcc ctgcatttta acaatccgac
      961 gaaagtccga cggtggacaa tttgttgaag gggaaggatc acgaaccgcc atgctcgcgc
     1021 gtggactcgc ctttgccgac atcaatcccc ataaaaactt tgcgtatata gaccttgaag
     1081 aggatctgca agtgccaagc atcgaagaag cagctcgcgc ctttggcacg cgaattattc
     1141 gcagcgtgca taatatggaa gggccagaaa ccgacatccg cgcccgagtg caaagcctgc
     1201 gaagaaccaa ggacgagatc gctaaaattg cctttacacc ccgaaatctt tcggacgtta
     1261 ccaagctctt taaagacgca aagtgctgtc aaagcgaaga atacatcctt atcgcaatgg
     1321 gccaatacgg actgccgtca cgcatccttt ctccactgct gggctcctcg gtctgttttg
     1381 tctcaagcga ggaatcaatt cgcaccagtg gcaaccccct gggacagctg gacccggtca
     1441 cccttaacga ggtgtaccgg ttcagggaaa tttctgcttc cacacgggtg ttcggcatta
     1501 ccggcaaccc gctcgcggca acctcaagcc cggctataca caacaagggc tatcgcgtcc
     1561 aaggcctgga tgcggtgtat ataccgataa aggcagaaaa cgcggaagag gctatcgagt
     1621 tcgcagagga agtgggtata accggactct cggtaacctt cccctttaag gaaagcgtta
     1681 ttcccaatct ttcggagata tccgccaaca ccggagaaat tggcgcgtgc aacaccatcg
     1741 taaaaagcgc acgcggttgg attggatata ataccgacga agcaggtttt tcccgagccc
     1801 tcctccaatt ccttgggaaa aaggacctta aacgggtgcg ggtttcaatt atcggcgccg
     1861 gcggggccgc acgcgcgatt acccatgcga ttaaacaact tggcgccaaa g
//
LOCUS       DAOQXQ010000364         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71148_length_1911_cov_2.705819, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000364 DAOQXQ010000000
VERSION     DAOQXQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71148_length_1911_cov_2.705819"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PKH81_06400"
     CDS             complement(<1..1368)
                     /locus_tag="PKH81_06400"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNQ97702.1"
                     /translation="MIVTDGRNAARIVPAILGSLLLLGSAFIALSLLLAIFPLTLAAT
                     RFAYVGNLLFGVYGYSSFLVPLFFLSSAILMLLPLRSFRSACLLGGSVVPFFTVVAAE
                     RLYAIAGLAGSSSSFAQGVSIAFALTTLLAVAVEYILLSSLVDRLRRHLDKSPALVKV
                     QQIQPVQPVQPVQMEEEWQDEPEALVEIEVQDEALAEPESEETIEDVPEYIDFEKMRA
                     PFDLPQSQDWVEPSATQESVPHPEEPAFEGEIDFSEEEALSELEAFESDDADEEEDLL
                     DEDEIVDEEEILDEDEKILDEEELVDEENSADEELCDELAGVECIHGTPSPSVSEPKV
                     ALLKKKKTYEIPYEVLNTYPDGEYWIIDEATRRSAAALKETLNEFHIQADVTGIRKGP
                     VITMFEILPAPGVKLSKIVGLQDNIALRLAASSVRIVAPIPGKHAVGIEVPNQRRAIV
                     SFREII"
     gene            complement(1401..>1911)
                     /locus_tag="PKH81_06405"
     CDS             complement(1401..>1911)
                     /locus_tag="PKH81_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNQ97703.1"
                     /translation="TGVLGFALKDLVENLSPFWISCALVVTGLLLLLSGKIRQENASA
                     RVTIRQGLIVGLAQGIGVIPGISRSGSTISASLFVGMDRATAGEFSFLLSIPAILAAF
                     ILELKSADTLYGTVSLPALIAGMLSAFVVGYLALRFLLALIRKGRLAWFALYLIPAGI
                     TLAVYFSRT"
BASE COUNT          529 a          502 c          476 g          404 t
ORIGIN      
        1 gataatctcg cggaagctta caatggcgcg acgctggttt ggaacctcaa tacccaccgc
       61 atgttttccg ggaatggggg caacgatacg tacactggaa gctgcgagcc taagggcaat
      121 gttatcctgc aaaccaacga tcttggaaag cttaaccccg ggagcgggaa gaatctcgaa
      181 catggtaatt accggaccct ttcggatgcc ggtaacatca gcttgaatat gaaactcgtt
      241 cagggtttct ttaagggctg cggcgcttct tcgggttgcc tcatcaataa tccagtattc
      301 cccgtcagga taggtattaa gcacctcgta aggaatctcg tatgtctttt tctttttaag
      361 aagcgcaacc ttgggctcag atacagacgg agagggagtg ccgtgaatgc actccacacc
      421 ggcaagctca tcgcaaagct cctcgtctgc actgttctct tcgtctacga gttcttcctc
      481 gtcgaggatt ttttcgtctt cgtccaggat ttcttcttcg tctacgattt cgtcttcgtc
      541 caggaggtct tcttcctcgt ccgcatcgtc gctctcaaaa gcctccaact cggaaagggc
      601 ttcttcctcg gaaaaatcta tctcgccttc aaacgccggc tcttccggat gcggaaccga
      661 ctcctgcgtc gccgatggtt caacccaatc ctgactttgg ggtaagtcaa acggggcgcg
      721 cattttttca aaatcaatat actcgggaac atcctctatt gtctcttcac tttccggttc
      781 agcaagggct tcatcttgaa cttcgatttc aaccagagct tcaggctcat cctgccattc
      841 ctcttccatc tggaccggtt ggaccggctg aaccggttgg atctgctgga ccttcaccag
      901 tgcaggggac ttatccagat gtcgtcgaag ccgatcgacc agggacgaaa gcaatatgta
      961 ctcaacagct acggcaagca gggtagttag cgcgaaagct atagatacgc cctgtgcaaa
     1021 ggaggaactg cttccggcaa ggcctgcaat ggcatacagc cgttcggcag caaccactgt
     1081 aaaaaagggt accaccgagc cgccaagcag gcaggcagag cgaaacgacc tgagcggaag
     1141 aagcataaga atggcggaag aaaggaaaaa aagcggtaca agaaaggacg aatagccgta
     1201 aaccccaaac aacagattgc ccacataggc aaagcgagtt gccgccaggg ttagcggaaa
     1261 tatagccaac aacagggaaa gagcgataaa ggcggagcca agcaacaaca gcgagcccag
     1321 tatggccggc acaatacgag cggcgttccg tccgtcagta acaatcatgt aaaaaactcc
     1381 cacaagctag tatggcgctg tcaggttcgg gaaaaataga cagctaaggt gattccagcg
     1441 ggtatcagat acaaggcgaa ccaggcaagc ctgccttttc gtatcaatgc caaaaggaat
     1501 cgtaatgcca ggtaccctac cacaaatgcg gacagcattc ccgcaatcag tgccggtagg
     1561 gataccgtgc catagagagt atcggcagat tttagctcaa ggataaacgc tgcgagtata
     1621 gcaggaatag ataaaagaaa agaaaattct ccggcagtgg cccgatccat acccacaaac
     1681 agggaggctg aaatagtgct gccagagcgg gaaattcccg gaatgacccc aatgccctgg
     1741 gcaagaccca caatcaagcc ttggcgaatc gttacccttg cagatgcatt ctcctgcctg
     1801 attttaccgg aaagaagcaa taaaagtccg gttaccacca gggcgcagga tatccaaaac
     1861 ggagaaaggt tttccacaag atctttaagc gcaaagccaa gcaccccggt t
//
LOCUS       DAOQXQ010000365         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71377_length_1907_cov_3.394168, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000365 DAOQXQ010000000
VERSION     DAOQXQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71377_length_1907_cov_3.394168"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKH81_06410"
     CDS             <1..712
                     /locus_tag="PKH81_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNQ97704.1"
                     /translation="LRANGIAETIIVAVDGTNALGGSFFANSLVTGNWEDLIVEETVA
                     LIDAKFKTIAEPAARLLSGFSMGGFASWNLALKHPEVFSLAWVCCPGAWDNTGLSDTL
                     SGWNSTYRNAYGAAYAPDPSLPFPHAQIPSLDNTDNDLIIQQQWNDGFGGIQSRLQEY
                     SAKKERLTAVCLAYGSQDEYRWIPRGTRYIAQSMTDEGIPTTVREFSSGHRLTNAMIA
                     DSFIPFVREHLIVDHKNK"
     gene            747..1733
                     /locus_tag="PKH81_06415"
     CDS             747..1733
                     /locus_tag="PKH81_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ97705.1"
                     /translation="MAIIEVDHLCREFTYYEKAEGVTGSLRNLFARKKRVKNAVNSIS
                     FTIEEGATVGFLGPNGAGKTTTLKMLSGIMHPSAGDARVIGYVPWQRKKEFRRSISIV
                     MGQRSQLWPDLPALESFNLNRSIYEIDRREYAQTLEELISLFGVEAQLPVQVRRLSLG
                     ERMKMEIIAALLHRPKVLYLDEPTIGLDLISQKAIRDLLKTLKARFGTTVMLTSHYLS
                     DIEDLCERIILINQGIVMYDGSLAGVNATLADRKRINLVFSTGVPLSTLQDFPGFSCA
                     QDFSASFEVPRSEVRAFSQRALQSLPVIDLTIEDTPLEEGIEKLYRTEASHG"
     gene            1726..>1907
                     /locus_tag="PKH81_06420"
     CDS             1726..>1907
                     /locus_tag="PKH81_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97706.1"
                     /translation="MARALLARKYTDCAITGLRTALEYRVNFFLTLAGAIAPVIIQTI
                     LWIALYGDDPSTSFFG"
BASE COUNT          510 a          443 c          424 g          530 t
ORIGIN      
        1 acttcgagct aatggaatcg cagagacaat catagtcgct gtagacggga caaatgcctt
       61 gggcggttct tttttcgcca actccctggt taccggaaat tgggaagacc tgatcgttga
      121 agaaacggtt gcgttaatag acgccaaatt taaaactatt gccgagccag ctgctcgcct
      181 tctttcgggt ttctctatgg gtggttttgc ctcatggaac ctggctttaa agcatcccga
      241 ggttttctcg ctcgcatggg tgtgctgtcc cggcgcgtgg gacaacaccg gtttatccga
      301 tacactgtct ggatggaatt ctacctaccg caacgcttac ggtgcggcct atgccccaga
      361 cccttccctt ccctttcctc atgcacaaat tccgtccctt gataatactg acaacgattt
      421 gattatccaa caacaatgga atgacggctt tggcgggata cagtctcgtc tacaagaata
      481 ctctgccaaa aaagagagac ttaccgccgt atgtcttgca tacgggtcac aggacgagta
      541 tcgatggatt ccgcgcggaa cacggtatat tgcgcagtcg atgacagatg agggaatccc
      601 tacaacagtc cgtgaatttt cttccggaca ccgacttacc aatgcaatga tagccgatag
      661 ctttatcccc tttgtacgtg aacatttaat tgttgaccat aaaaacaaat agggtgtaca
      721 ctgctttcat ataaaggaag cagtgtatgg ctataatcga agttgatcat ctttgccgcg
      781 aatttaccta ctatgaaaaa gcggaaggag ttaccggttc ccttcgcaac ctatttgcca
      841 gaaaaaaacg tgtaaaaaat gcggtaaact cgatctcttt tactatagaa gaaggtgcaa
      901 ctgttggatt tttgggaccc aacggagcgg gtaaaacaac cacacttaaa atgctttccg
      961 ggataatgca cccaagtgcc ggagacgcgc gggttattgg ctatgtacca tggcaaagaa
     1021 aaaaagagtt ccgaagaagc atctctatag taatgggtca gcgaagtcag ctgtggccag
     1081 atttgcctgc acttgaatca tttaacctca atcgttcaat ctatgaaatt gacaggaggg
     1141 aatatgcgca aactcttgaa gagttaatta gcctgtttgg tgtagaagca caacttccag
     1201 tacaagtcag gcggttatcg ttaggagagc gaatgaagat ggagattatc gcagcccttc
     1261 tccatagacc aaaggtcttg tatctggatg agccgaccat tggccttgat ctgatttctc
     1321 aaaaagccat tcgtgattta cttaaaaccc ttaaagcccg attcggcact actgtcatgc
     1381 ttaccagcca ctatttatcc gatattgaag acttatgcga acgaataatc ctgattaacc
     1441 agggaattgt catgtacgat ggctctcttg ccggagtaaa cgcgaccctt gctgatcgaa
     1501 aacgtatcaa tctggtattc tcaaccggtg tacccctttc tacattgcag gacttccccg
     1561 gattctcatg cgcacaggat ttctctgctt cctttgaagt tccccgttca gaagttcgcg
     1621 ctttttctca gcgtgctctg cagtcattac cagttattga tttaactata gaagatacgc
     1681 cacttgaaga gggtattgaa aagctgtatc gaacagaggc ttctcatggc tagagcattg
     1741 cttgcgcgta aatatacaga ctgtgccata acaggattac ggacagccct tgaataccgt
     1801 gtaaatttct ttttaaccct tgctggagca attgcgccag ttattattca aaccattctc
     1861 tggattgccc tgtatggaga tgatccttct acttcttttt ttggttt
//
LOCUS       DAOQXQ010000366         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71514_length_1905_cov_3.198378, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000366 DAOQXQ010000000
VERSION     DAOQXQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71514_length_1905_cov_3.198378"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKH81_06425"
     CDS             complement(<1..275)
                     /locus_tag="PKH81_06425"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ97707.1"
                     /translation="MLYLLLAPFALMILIISTLFAVINHRNRRFTSLIFFLITTTSII
                     LFNMLELHADTNNELLVFSRITYGLLALLPVTWIYFCLEYARGGSLP"
     gene            771..1094
                     /locus_tag="PKH81_06430"
     CDS             771..1094
                     /locus_tag="PKH81_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ97708.1"
                     /translation="MSNDTTIQSVLDGDDVSLYDLGDFFRVLGDSTRIRIISLLIPSE
                     LCVQDIADSVGMSESAVSHQLRILRQAKMVRNRREGKQVFYALDDDHIEQLVRIGLAH
                     VKEGR"
     gene            1096..>1905
                     /locus_tag="PKH81_06435"
     CDS             1096..>1905
                     /locus_tag="PKH81_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97709.1"
                     /translation="MSCNCDGACDSVVRRSRRAVRLLVLRFVISSLLFAMAFLVPGDK
                     GSYIFIISWFLSGYTVLFSAIRGLRNKKIFDENFLMSIATIGAFATGQWSEGAAVMLF
                     FNLGEMIQESAVNRSRSSIEKCLDIRSNTARIHPDGSIVHPSQVAIGSLVMVLPGERV
                     PLDGLLVEGSSSFDTSAITGESIPRDLAAGDEVRISFDRGKEDFHHPMLRLVWEDDYL
                     IVVDKRNGLLSMSTDKIKEKTAYHLLSDYVKKSSPENKIFILHRLDRDTSGL"
BASE COUNT          509 a          328 c          471 g          597 t
ORIGIN      
        1 tgggggagag atcctccacg ggcatattct aaacagaaat aaatccaggt aaccggtaag
       61 agggcgagta ggccatatgt aatgcgtgaa aacacgagaa gctcattatt agtgtccgca
      121 tgtaattcga gcatattaaa aagaatgatt gaggtagtcg ttattaaaaa gaagatgagg
      181 gaggtaaagc gtctgtttcg atggttaatt actgcaaaaa gggtagagat gatcaggatc
      241 atgagtgcaa atggggctag aaggagataa agcattggct ggtgcatcat gggtgcctga
      301 aatgactgct cgcaggattg ttggtaattc tacggagcgg atctttgatt agaaaggacg
      361 aaactgcgac agtccaaggt ttgtatccca aaacaacctc tttacctatg tatgggtata
      421 ctttatcccg gaatttccct ctgttcaata aaaaatcgag aagtaatctg ttttttaagc
      481 agtacatgat gcgccttctg cggagatagg gaggagttac caacaatcct gcgagcacta
      541 aaaaaagcta gccgcgacat atatatgccg cggctaaagg tatttttaac tagctgttta
      601 gcttgaatgc attctacgcc cgttattagt atcaagcaag gttatgaacg ggtgattttc
      661 gggtgatttt ttgcaattgt aacaatgtaa atgggatttg tttacaaatg accctctttt
      721 ttgaggcgat tatgcttgaa tatatgaaca tatgaatata tattcatatt atgagtaacg
      781 atactacgat tcagtctgtg ttagacgggg atgatgtttc cctttatgat ttgggtgatt
      841 tttttagggt tcttggggat tcaacgagga tcaggattat ttccttatta attccctcgg
      901 aattgtgtgt gcaggatatt gcagattctg tcggcatgtc tgaatctgcg gtttcgcatc
      961 agttacgtat tttgcgccag gctaaaatgg tacggaatcg tcgtgagggg aaacaggtgt
     1021 tttatgctct ggatgacgac catattgaac aattggttcg tattggtttg gcacacgtta
     1081 aagaaggaag atgatatgag ttgtaactgc gatggtgctt gtgattctgt ggttcgtcgc
     1141 tctcgtcggg cggttcgtct gctggttctt cgatttgtta tatcttcact tttattcgcc
     1201 atggcttttt tggtaccagg cgataaaggt tcgtatatct ttattatttc atggtttctt
     1261 tccggatata ctgtgttatt ttccgcgata cgtgggttac gaaataaaaa gatttttgat
     1321 gaaaactttt tgatgtcaat cgcaacgatt ggagcattcg caacaggtca gtggtctgaa
     1381 ggtgctgcgg ttatgctctt ttttaatttg ggagagatga ttcaggaatc agcagttaat
     1441 cgttcacgct cttctataga aaagtgtctt gatattagat ctaataccgc aaggattcac
     1501 ccggatggtt cgattgttca tccttctcaa gttgctattg gatctcttgt gatggttctt
     1561 ccgggcgaga gagttccact cgatggcctt cttgtggaag gatcctcttc gtttgatact
     1621 tcagcgatta ccggagaaag tataccccgt gatcttgctg ccggcgatga agtgagaatc
     1681 agctttgacc gtggaaaaga agactttcat cacccgatgc ttcggttggt atgggaagac
     1741 gattacctta ttgtcgtgga caagaggaac ggattactat ctatgtcgac cgacaagatc
     1801 aaggagaaaa cggcttatca cttgctaagc gactatgtta aaaagtcaag tcccgaaaac
     1861 aagatattta ttctgcatcg tctggaccgc gatacttccg gttta
//
LOCUS       DAOQXQ010000367         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71578_length_1904_cov_3.190914, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000367 DAOQXQ010000000
VERSION     DAOQXQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71578_length_1904_cov_3.190914"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1904
                     /gene="fusA"
                     /locus_tag="PKH81_06440"
     CDS             <1..>1904
                     /gene="fusA"
                     /locus_tag="PKH81_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ97710.1"
                     /translation="GNTTTDWMDQEKERGITIQSATITCFWNSKQINIIDTPGHVDFT
                     AEVERSLRVLDGAVAVLCAVGGVQPQTETVWRQADQYRVPRVCFVNKMDRVGADFYAV
                     IEDVHQKFGATCLALQIPIGQGSDFEGVIDLIDMKELRWDEATAGEAILESPISSDRL
                     GEATQWREKLLDGLSAFSDELTELILEGAEIPAQLIRSCIRKSVLARDIIPFLCGASR
                     RNQGVQPLVDAVVWYLPAPHEVPPAIGFQTKKEEDISIPCSATGIPLGLVFKIQWDRE
                     AGSLCYVRMYSGKIKTGDQIYNVSKKKRERVNRILRMHSNKSEPMDSLEAGDIAVFIG
                     LKLAQTGDTVGSEGMPVLLERMHFPEPVISVAVEPKTLSERDKLKDTLEVLSREDPTF
                     TSREDSETGQLIISGMGELHLDVLVTRMLDDFKVEARVGNPQVTYRESVTATIEHTER
                     FSKNIGGKDTAAGVTLRVEPLPRGSGNTFRSTIKKSAYPEDMLDAVERAVTNAFGSGI
                     QWGYPCVDIGVTLVDIEYNELTATTFAYEAAASMGFDEACRKASPELLEPVMNVDILC
                     PKEFVGEAMSQVTQRGGLISSLESKPSIEVVHAQAPMAKMFGFSTSLRSVTQGRASFA
                     MSFSHFEVKK"
BASE COUNT          470 a          421 c          526 g          487 t
ORIGIN      
        1 atggtaatac gactactgac tggatggatc aggaaaaaga gagaggcatt accatacagt
       61 ctgcaacgat tacctgtttc tggaatagta agcaaatcaa cattattgat actccggggc
      121 acgtcgattt taccgcagag gtagaacgat ccttacgggt gctagacgga gccgttgccg
      181 ttttatgcgc ggttggaggg gtacagcctc aaaccgaaac agtgtggcgc caagccgatc
      241 agtatcgagt gccccgcgtg tgttttgtta acaagatgga tcgggtaggg gccgattttt
      301 acgctgttat cgaggatgtt caccaaaaat ttggcgccac ctgtcttgcc ttgcaaattc
      361 cgattggaca gggaagtgat tttgaaggtg tcatcgactt aattgatatg aaggaattgc
      421 gttgggatga ggcgactgcc ggggaggcca ttctggaaag tcccatttcc agcgatcggc
      481 ttggcgaagc aactcaatgg cgggaaaaac tgcttgatgg tctttcggct ttctccgatg
      541 aattgaccga gcttatcttg gaaggcgctg agattcctgc acagttgatt cgctcttgta
      601 ttcgaaaaag cgtcttagcc cgcgacatta ttccctttct gtgcggcgca tcccgtcgaa
      661 accagggagt gcaacccctg gtcgacgcag ttgtgtggta tctgcctgcc ccgcatgagg
      721 ttccacccgc catcggattt caaacaaaga aagaagagga tataagtatc ccctgttcag
      781 ccacgggtat accgcttggt ctggtattca agatacagtg ggatagagaa gctggttctc
      841 tctgttatgt gcgcatgtat tccggaaaga ttaaaacggg cgatcaaatc tataatgtaa
      901 gcaagaagaa acgtgagcgg gtaaaccgta ttttgcgtat gcattccaat aaaagcgagc
      961 cgatggattc attggaagcc ggggatatcg cggtgtttat cggccttaag cttgcgcaga
     1021 caggcgatac cgttggaagc gaaggaatgc cggtgttgct tgagcgtatg cattttcctg
     1081 agccagttat ttccgtcgcg gttgagccaa aaaccctttc tgaacgggat aaacttaagg
     1141 ataccctgga ggtgctttcc agggaggatc ctacctttac cagcagagaa gacagcgaaa
     1201 ccggacagct tattatctct ggaatgggag agcttcattt agacgtactg gttacccgta
     1261 tgcttgatga ctttaaggtg gaagcccgtg ttggcaatcc tcaggttacc tatagggaat
     1321 cggttaccgc gacgatagag catacagagc gtttttcaaa gaacattggc ggaaaggaca
     1381 ccgcggcggg tgtaacccta cgcgtagagc ctctaccgcg gggaagtggt aataccttcc
     1441 gctccacgat aaaaaagagc gcttatccag aggatatgtt ggacgcggtg gaacgcgcgg
     1501 taactaatgc ctttggctcc ggtatccaat ggggttatcc ctgtgtggac attggtgtaa
     1561 ctctggtgga tatagagtat aacgaactta ctgccacaac ctttgcctat gaggctgctg
     1621 cctctatggg ttttgacgaa gcatgccgaa aggcaagtcc tgagttgctt gaaccggtaa
     1681 tgaatgtgga tattctctgt cccaaggagt ttgtgggaga ggcaatgagt caggtaaccc
     1741 agcggggtgg acttatttcc agtctggaat ccaaaccttc catcgaggtt gttcatgctc
     1801 aggcgcccat ggcaaagatg tttggctttt ccacctcttt gcgctccgtt acccagggca
     1861 gggcttcttt tgccatgagc tttagtcact tcgaggttaa aaag
//
LOCUS       DAOQXQ010000368         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71931_length_1898_cov_2.824742, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000368 DAOQXQ010000000
VERSION     DAOQXQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71931_length_1898_cov_2.824742"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1103)
                     /gene="argC"
                     /locus_tag="PKH81_06445"
     CDS             complement(54..1103)
                     /gene="argC"
                     /locus_tag="PKH81_06445"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNQ97711.1"
                     /translation="MITAGIIGATGYVGVELVRLLLTHPLVSNITLSSVSFEGQNIVD
                     VYPNLRGMLRGKTDGTLMDADAVLEASDVVFTALPHGIAEKWAAACIEKGKKLIDLSA
                     DFRFDQDEATFKEWYKKDWEFPAVHAESVYGLPELQRSLIKSARIIGNPGCYVTAASL
                     ALIPALEKSLVATDLIIVDAKSGITGTGRNATDTNNFSDAGESFKPYNIGKHRHQPEI
                     ARNMSKAAGKPVSVLFTPHLLPMSRGIIATVYAPLTQEISIEKIHALYSERYADEPFV
                     RLLPIGSTAVTKNVRLSNYCDLSLHLVNGGKTLVVASAIDNMVKGAAGQAVQNMNLIS
                     GFPETAGIDHIPAAF"
     gene            complement(1373..>1898)
                     /locus_tag="PKH81_06450"
     CDS             complement(1373..>1898)
                     /locus_tag="PKH81_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97712.1"
                     /translation="GNSAYSRGRWLFSVLGRWEYTPEDPVSTDHPFSIVSLVTPALFL
                     RDSCGLSFGWERRGDDEDDIYLSWARGYGTTDALEAWKASAQYTVPIFHPDVSLGALL
                     YFSRISLTGFYDQRQELHGSEKQVSAGAELLFHFYLFQLALELNAGVRYSRLLDEQLN
                     RYEFVLLGVPLVRY"
BASE COUNT          517 a          466 c          446 g          469 t
ORIGIN      
        1 cgcatacccc tccgtctata tagttcatac tcggtatctc cttatgtaca ccactaaaag
       61 gctgcgggaa tatgatcgat acccgctgtc tcggggaagc cggatatcag gttcatattt
      121 tgaactgcct gtcccgccgc tcccttaacc atattgtcta tcgcgcttgc cacaaccagg
      181 gttttccccc cattaaccag atggagagaa agatcgcaat aatttgaaag gcgaacattc
      241 ttggtaaccg ctgtagagcc aataggtaag agccgtacaa agggctcatc tgcataccgt
      301 tcgctataca gtgcgtgtat cttctctatg ctgatctcct gcgttaaggg tgcgtacact
      361 gttgcgataa taccgcggct catcggaagc agatggggag taaacaaaac tgaaaccggt
      421 tttccggctg ccttgctcat attccgggct atttctggtt gatggcgatg tttaccaata
      481 ttgtagggtt taaaggattc gcccgcatct gaaaagttat tggtgtccgt agcgttcctg
      541 cctgtcccgg taatgccgct tttagcgtct acgataatca aatcggtagc gacaagggat
      601 ttttccaaag caggaataag ggcaagggaa gcggccgtta cataacagcc tggattcccg
      661 ataatccgtg cagacttgat aagcgaccgt tgtaattcag gaagtccata cacggattca
      721 gcatgaactg cgggaaactc ccaatccttt ttgtaccatt ccttaaaagt cgcctcatcc
      781 tggtcaaacc gaaaatcagc agagaggtca ataagctttt ttcccttttc gatgcaagca
      841 gcggcccatt tttcggcgat accatgcgga agagcggtaa agactacatc agaggcctct
      901 agaacagcat cagcgtccat cagcgtccca tcagttttcc ctctgagcat tccccgcaaa
      961 ttcggataga cgtccacaat attttggccc tcaaagctca cggagcttaa ggtaatgttg
     1021 gacacaagtg ggtgtgtcaa taatagacgc accagttcta cgccaacgta tccggtggcg
     1081 ccaataatgc cggcagtaat catagataac tccagattat acagtatttg aaagtggttc
     1141 aagaccgttc gcaaccggaa cggggcgagg attcagcttg attacgcctg acggcgacgg
     1201 cggagaagga tgacaggggg attgaatgtc ggtgaacaca tgttcatatc gcttctcctt
     1261 tgaagggttg tattaggtgt atttttaatg ctaaccacac aaaaggtcaa ggggttttat
     1321 gtattaaaat acactattta tgtataaatt tacgtttcta cctgaatctc ggttaatagc
     1381 gtaccaaggg gactcctaaa agaacaaatt cgtagcgatt aagctgttca tcaaggagtc
     1441 gtgagtagcg tacccctgcg ttcaattcaa gtgcgagctg aaaaagataa aaatgaaaga
     1501 gtaactcggc tcctgccgac acctgcttct cgctaccatg aagctcctgc cgctgatcat
     1561 aaaagccggt aaggctgata cgggaaaaat agagcaaggc gcccagagat acatcaggat
     1621 gaaatatcgg tacggtatac tgtgccgagg ccttccatgc ttccagtgca tccgttgttc
     1681 cgtatcctcg cgcccaagac agatagatgt cgtcctcatc atccccgcgt ctttcccatc
     1741 caaaagataa cccgcatgaa tcacggagaa aaagagcggg cgttaccagg gagactatac
     1801 taaacggatg atcggttgaa accgggtcct ccggcgtgta ctcccaacga cccagaacgg
     1861 aaaaaagcca tcttccccga ctataagcgg aattcccg
//
LOCUS       DAOQXQ010000369         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_71977_length_1897_cov_3.373507, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000369 DAOQXQ010000000
VERSION     DAOQXQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71977_length_1897_cov_3.373507"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..875)
                     /locus_tag="PKH81_06455"
     CDS             complement(63..875)
                     /locus_tag="PKH81_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ97713.1"
                     /translation="MKSLESKITVITGGASGIGRMLALQMASQGSRVVVWDINQELLS
                     SLEREAAEKKVSIIGMALDITDRKRVYACAQEVLSRVGPVDILVNNAGVVSGQTLLET
                     PDEKIEKTIQVNFLSNCWTVKAFLPSMIERNTGHIVTIASAAALIGVTGLADYSASKS
                     AVYGFHESVRMELKKQRKKIATTVVCPFFIDTGMFAGVQTRFPLLLPILRPEYAVRRI
                     NKAILAKKERLIMPRFVYSVFLLKLLPVWLMDVIADFFGITHAMDQFTGRRG"
     gene            954..>1897
                     /locus_tag="PKH81_06460"
     CDS             954..>1897
                     /locus_tag="PKH81_06460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ97714.1"
                     /translation="MKFTEFTLDSGLQEGLAEAGYETCMPVQEQVLKNGLDGADLYVQ
                     SQTGTGKTAAYLVTILQRLVNDPSLAGKKALIMVPTRELAVQVEEEAKIVGSCMNIKS
                     ASFYGGVGYGNQQDLLRKGVDIIIGTPGRVIDLQESGSMKLDQVAFLVIDEADRMFDM
                     GFYPDLRKLIKVLPQTGKRQTMLFSATLNVSVKNLAWEYTINPREITIEAENVTVDEI
                     DQLLFHISSDQKMKLLLGLIVKEKPDSLIIFCNTKRMSEIVAKRLKINGYEADFIIGD
                     LPQSKRLQVIDSFKSGSLKMLVATDVAARGIDVDSLSMV"
BASE COUNT          559 a          450 c          402 g          486 t
ORIGIN      
        1 aattacacca ggccgggcat gctcaatatc ttgtccgaca ggaaagaccg taacgctcgc
       61 acttacccac gccgaccggt aaattgatcc atggcgtggg taatcccaaa gaaatcagca
      121 atcacatcca tgagccacac cggcaaaagc ttaagcaaaa acacagaata gacaaaccgg
      181 ggcataataa gtcgctcttt tttagctagt atagctttat tgattcgcct taccgcatat
      241 tctggacgca agataggtaa tagtaacgga aaacgagttt gaacaccggc gaacataccc
      301 gtatcgatga agaaaggaca caccacggtt gtcgcgatct tttttcgctg cttttttaat
      361 tccatacgta ccgattcatg aaatccatat acagccgatt tactcgctga ataatcggca
      421 agaccggtta ccccaatcag ggcggctgca gaggctatcg ttacaatatg accagtattt
      481 cgctcgatca tggacggtaa aaaagccttt accgtccaac aattggaaag aaaattgacc
      541 tgtatagttt tttcgatttt ttcgtcaggt gtttctaaca aggtttgtcc agagaccacc
      601 cctgcgttat tgaccagaat atcaacaggt cccacccggc ttagtacctc ttgagcacag
      661 gcatacaccc gcttcctgtc ggtaatatca agggccatac cgataatact cacctttttt
      721 tcggcagctt cacgctccaa actggatagt aactcctggt ttatgtccca aaccacaaca
      781 cgagatccct gtgaagccat ctgcagggca agcattctcc caataccgct tgcccccccg
      841 gtaatgactg ttatctttga ttcaagtgat ttcatcccgc cattgtatca tggatctgtc
      901 cggaggggac gaatctattc acgcattttc atttatgcta ttatcgaaga tacatgaaat
      961 tcaccgaatt taccctagac agcgggctcc aggaaggact cgcggaagca ggatacgaaa
     1021 cctgcatgcc tgttcaagaa caggtactta aaaatggatt ggacggagct gatttgtatg
     1081 tccagtccca aacaggcacc ggcaaaaccg ccgcctacct tgttaccatc cttcagcgat
     1141 tagtgaacga tccctcgctt gcaggaaaaa aagccttaat catggtcccg accagagaac
     1201 ttgccgtaca agttgaagaa gaagccaaaa tagtgggatc gtgtatgaat attaaatccg
     1261 ccagctttta tggtggggtc ggctacggaa atcaacaaga tctacttcgt aaaggcgtgg
     1321 atatcattat tggtaccccc ggccgggtaa ttgacttaca agaatcaggt tcaatgaagc
     1381 ttgatcaagt agcttttttg gtaatagacg aagcagatcg tatgtttgac atgggctttt
     1441 accctgactt acgcaagctc attaaggttc ttccccaaac aggaaaacgt caaacaatgc
     1501 tttttagcgc aacgttaaat gtctcggtta agaaccttgc gtgggaatac accattaacc
     1561 cgcgcgaaat taccatcgaa gcagaaaatg ttacggtgga cgaaattgac caactgttgt
     1621 tccacatctc gagcgatcaa aaaatgaaac ttctgttggg attgatcgta aaagagaagc
     1681 ctgacagtct gataatcttt tgtaatacca agcgaatgag cgagatagtt gccaaacgct
     1741 taaagataaa tggatatgaa gcagacttta ttattggcga cttgcctcaa tcaaaacgtt
     1801 tgcaggttat tgactcattc aaatcgggat ctcttaaaat gcttgttgca acagacgtag
     1861 ccgcacgagg catcgatgtt gattccctat ccatggt
//
LOCUS       DAOQXQ010000370         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72051_length_1896_cov_3.186312, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000370 DAOQXQ010000000
VERSION     DAOQXQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72051_length_1896_cov_3.186312"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKH81_06465"
     CDS             complement(<1..207)
                     /locus_tag="PKH81_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HNQ97715.1"
                     /translation="MANPKLFRVTDHAVHTYRMIEEGDRILVGASGGKDSTALMEYLG
                     NRRKRRSGPRFEVCALHVKTDIGSP"
     gene            complement(220..1047)
                     /locus_tag="PKH81_06470"
     CDS             complement(220..1047)
                     /locus_tag="PKH81_06470"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNQ97716.1"
                     /translation="MNSKPDFSDAKRALSELQYKVTQEGHTEPPFQNDYWDTNEEGLY
                     LDIVSGELLFTSHDKFTSSCGWPSFTRPATADALRELSDTSHGMVRTEVRSTRSDSHL
                     GHVFDDGPAPDHRRYCINSAALRFVPASRSTAYVGAGCFWGVEASFARLPGVLSTRVG
                     YAGGHSPNPTYQQVCTGDTGHAEVVRIYFDPDKITYEELLSHFFQIHNPTTVNRQGPD
                     VGSQYRSIILTLSPEQEDAAAEVMRSLSPAFDSPIVTELYPFTEFYEAEDYHQGYYL"
     gene            1078..>1896
                     /locus_tag="PKH81_06475"
     CDS             1078..>1896
                     /locus_tag="PKH81_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNQ97717.1"
                     /translation="MDLEMLKFRSECIHQTRTFFRNKHYLETDTPALAPYLIPETCLE
                     VFTTDYQKSGEAIIQPLYLVPSPEVYIKQLIADHGVSIFQISKCYRNGESVGRIHSPE
                     FTMLEYYTMGADYRQSLELTESFINTLIQSIAQAIPLHLPFERITMDEAFMQGAGFSL
                     ASATESRDLALHAERLGLGDSNDYQGWAWDDLYELLLVHAVEPFLANKEAVFVTDYPA
                     RVPTLAQERTVSQASIQWQVKERWELYIRGVEIANCYTEERNTDRVDAYIREEGA"
BASE COUNT          489 a          481 c          481 g          445 t
ORIGIN      
        1 gggtgagcca atgtcggtct ttacatgcaa ggcacatact tcaaatcggg gaccagagcg
       61 ccgcttgcgt cgattaccca agtattccat aagcgcggta gaatcctttc cacccgaagc
      121 tcccaccaaa atccggtcgc cttcttctat catcctgtag gtgtgcacag cgtggtcggt
      181 cacgcgaaag agtttcgggt tagccatgag tcgatactat caaaggtaat agccctgatg
      241 atagtcctcg gcctcgtaaa attcagtaaa cggataaagt tcagttacaa tcggcgaatc
      301 aaaggcgggc gacaaggagc gcatcacttc tgcggccgca tcttcctgtt ccgggctcag
      361 ggtaagaatg atggatcggt actgagagcc aacatcgggt ccttgccgat tcacggttgt
      421 tggattgtga atctgaaaaa aatgggatag aagttcctca taggtaattt tatcaggatc
      481 aaaataaatg cgcacaacct ccgcatgtcc tgtatctccc gtacacacct gctgataggt
      541 tggattggga ctgtggccac ccgcataccc cactcgtgta gacagtacac cgggaagacg
      601 ggcgaaggat gcttctactc cccaaaaaca gcccgcacca acataggcgg ttgaccggga
      661 agcgggcaca aaacgaagag ctgccgagtt aatacaatag cggcggtgat cgggagcagg
      721 accgtcgtca aacacatgac cgagatggga gtcagagcgc gtgctacgga cttctgtgcg
      781 taccatacca tgcgaggtat cgctcaattc gcgaagtgcg tcggcagtgg cagggcgggt
      841 aaaactgggc cacccgcagg aagaagtaaa tttatcgtgc gaggtaaaaa gaagctcacc
      901 tgaaacaata tcaagataga ggccctcttc gtttgtatcc cagtagtcgt tttgaaaggg
      961 gggctcggta tggccttcct gagtaacttt atattgaagc tcactgagcg ctctctttgc
     1021 atcgctaaaa tccggtttgc tgttcatagg cagcctcctt gtgctaatat actgattatg
     1081 gacttggaaa tgctcaaatt ccgcagtgaa tgtatccacc agacgcggac tttcttccgg
     1141 aacaagcact atctggaaac cgacacgcca gcccttgctc cctatcttat tccggaaacc
     1201 tgtctggaag tatttacaac cgactatcaa aaaagcggag aagccatcat tcaaccgctg
     1261 tatttagttc cttctcccga ggtgtatata aaacaactca ttgcagacca tggcgtttca
     1321 atctttcaaa tttccaaatg ctaccgtaac ggtgaatctg ttggaaggat acacagcccg
     1381 gagtttacca tgcttgagta ctacaccatg ggcgcagact accggcagtc tctggaatta
     1441 accgaatcgt tcattaacac ccttatccaa tccattgccc aggccattcc cctgcacctt
     1501 ccctttgaac ggataaccat ggatgaagcc tttatgcaag gtgcgggttt ttcccttgcg
     1561 agcgcaaccg aaagccgtga tcttgcgctg catgccgaac gccttgggtt gggggatagc
     1621 aatgattacc agggctgggc ctgggatgac ctgtatgaac ttctattggt ccatgccgtg
     1681 gaaccctttc ttgcgaacaa agaagcggtg tttgtaactg actaccctgc ccgtgttccc
     1741 accctggccc aggaacgtac ggtcagccaa gcctctatcc agtggcaggt taaagagcga
     1801 tgggagctct atattcgggg ggtagaaatc gctaactgct acaccgaaga gcgaaatacg
     1861 gatcgggtag atgcctatat tcgggaagaa ggggct
//
LOCUS       DAOQXQ010000371         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72244_length_1893_cov_3.493471, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000371 DAOQXQ010000000
VERSION     DAOQXQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72244_length_1893_cov_3.493471"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..913)
                     /locus_tag="PKH81_06480"
     CDS             complement(83..913)
                     /locus_tag="PKH81_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ97718.1"
                     /translation="MKKSLIMCTAILAGLWALMGCSPTKENTRVQAVQKAGKIILGTS
                     ADYPPYEFHAQMDGKDTIVGFDVALANEIAKDLGVTLEIKDMDFDGLLAALASGNVDF
                     VIAGMTPSEERRKNVDFSDIYYFAEHGVVIRTADESLYSVAPESLKDRMIGAQRGAIQ
                     VSLAKTRIKGVAEAEAENPHPQVKELGKLPDLIMELKNKKIDAVVVELPVARSYLKAN
                     SDLVLASYTFQDDDGGSAVAVKKGEKALVEAINKTILRLQDEGRIEAFVAEASELVEY
                     "
     gene            1012..1698
                     /locus_tag="PKH81_06485"
     CDS             1012..1698
                     /locus_tag="PKH81_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ97719.1"
                     /translation="MQEASVFKGTAIYQKITELDSVYTGIEDEQTEWKKVTPFHCPSG
                     CGSCCVDFEPDVMEVEALYLAAWMLHHQKERALSILDGSFVSPRPDPDSGCAFFDPES
                     PYHCTVYGGRALICRLFGYTGDRGKDGLGRWKPCKFLPENAEDGGEHRKRQYSSAELQ
                     ERFGATPPLMSDITARAIAIDPGTAGKRYPLMEALAIALRRLQLALRFLDAPPEPDSP
                     APDFPRPRAV"
BASE COUNT          447 a          503 c          454 g          489 t
ORIGIN      
        1 cttcatagta actccttaca cataccaaac cggtactaac tgaccttaat atggtgaaga
       61 aggaaacaaa tgtcaataaa ccttagtatt ccacaagctc gcttgcttct gccacaaagg
      121 cttctattcg accttcatct tgaagacgga gaatagtctt gtttatcgcc tccacaagcg
      181 ccttctctcc tttcttgacc gcaacggcag agccgccgtc atcgtcctgg aaggtgtaag
      241 aagcaagcac taagtcagaa ttcgccttaa gataggagcg ggcaaccgga agctccacaa
      301 caaccgcgtc tattttttta ttcttgagtt ccataatcaa atcgggcagc ttacccagtt
      361 ctttaacctg ggggtggggg ttctctgcct cggcttcggc gacacccttt atacgggttt
      421 ttgccagact tacctgaatg gctccccgtt gagctccgat catccggtct ttgagagact
      481 cgggagcaac actgtatagg gactcgtcgg ctgtacggat taccacccca tgctcggcaa
      541 aataatagat atcggaaaaa tcaacgttct tgcgtcgctc ttccgatggc gtcatccctg
      601 cgataacaaa atcgacgtta cccgaggcaa gggcggcaag aagaccgtca aagtccatat
      661 ccttgatctc gagagtaacg ccaagatcct tggcaatctc gttggccaga gccacatcaa
      721 aaccgacaat cgtgtccttg ccgtccattt gtgcatgaaa ctcgtacggg ggataatcgg
      781 cagatgtccc caggataatc tttcccgcct tttgaacggc ttgtacacgg gtgttttcct
      841 tcgttggact acaccccatc agggcccaca aacctgccag gatagcggta cacataatca
      901 gtgatttttt catagagcaa cctccatagt cagtgcgctg tatcctttga gtatagtcag
      961 atacgcattt ttttcgctat actgcgcatc ggttttggaa gaggttttac tatgcaagag
     1021 gcttcggtat tcaagggaac ggcgatatat caaaagatca ctgaattgga cagcgtatat
     1081 accggtatag aagatgagca aactgaatgg aaaaaagtaa ccccctttca ttgcccaagc
     1141 gggtgtggat cctgttgtgt cgattttgaa ccagatgtta tggaggtaga agccctctat
     1201 cttgcggcct ggatgctcca ccatcaaaag gagagagctc tctccatctt agatggaagt
     1261 tttgtgtccc cccgtccgga tcccgatagc gggtgcgctt tttttgatcc tgaaagcccg
     1321 tatcactgca ccgtctatgg cggtcgcgcc cttatttgtc gcctgtttgg ctatactggc
     1381 gaccggggta aggatggttt gggccgatgg aaaccctgca aatttcttcc tgagaatgca
     1441 gaagacgggg gagaacatcg taaaaggcag tatagctcgg ccgaattaca agagcgcttt
     1501 ggtgcaaccc cgcctctgat gagcgacatc actgcccgtg ccattgccat agatcccggc
     1561 accgcaggaa aacgctatcc gttgatggaa gccctggcca ttgccctgcg ccgtctacag
     1621 cttgcccttc gattccttga cgctcctccc gagcctgact ctccggcacc ggattttccc
     1681 cgtccccgag ccgtgtaaca cagacggcgt tttgcctcat tattccattt gcgccggcac
     1741 agtactcatg gtatcctgac aagcggagga ataccactga tgtctcattc gattatcggc
     1801 gttaatgaac gcccagctct tacgcgatgg gtaccgctga gttttcagca tgtgtttgct
     1861 atgttcggtg ccacggtgtt ggtgccgatg tta
//
LOCUS       DAOQXQ010000372         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72317_length_1892_cov_3.055525, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000372 DAOQXQ010000000
VERSION     DAOQXQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72317_length_1892_cov_3.055525"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKH81_06490"
     CDS             <1..350
                     /locus_tag="PKH81_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97720.1"
                     /translation="YEGFSKIRTRGLLSINDIIDVQRLLIQNDSGIRNSIGTALVNDR
                     THEVMYTPPDDYTVINALLKNFVKYRNGDGPTLAIVKDTLPGIYSKELVETLFVHPYC
                     KGEFLYIRNLSGY"
     gene            518..1276
                     /locus_tag="PKH81_06495"
     CDS             518..1276
                     /locus_tag="PKH81_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97721.1"
                     /translation="MIEDDLRNGHMTNEIWESWLAKLDETNKLLIEREWIEEPFSKET
                     KFYNIRPGCTDFEIKRIQETNLIRLPDDFIEVATRYSAGLEIARWECPVKDLPAEYNG
                     SYLKYHLWRLNGYFSSDPLWEIPDSKDLRWGGPHDTGWSTIEDFGEGHAEYVRIWSKR
                     GYCLNNLGNGDMIAIDLSWETEAAPVLYFSHDDPDLHGIRLGYSFIDYIDRIISFCCA
                     DPEQIAKEAYDSKQHIIDTDKILKEWKRQLLDVK"
     gene            1356..1601
                     /locus_tag="PKH81_06500"
     CDS             1356..1601
                     /locus_tag="PKH81_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HNQ97722.1"
                     /translation="MSTLYNVNAPKRPTNLTVNSDLLTQAKDYKINVSATLETALAEA
                     LKSKKVDLWKEENREAIQSYNIILEEYGLFSDEIRMF"
     gene            1601..>1892
                     /locus_tag="PKH81_06505"
     CDS             1601..>1892
                     /locus_tag="PKH81_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HNQ97723.1"
                     /translation="MSQYHVYENLNEKSKKLYPYVIDVQSTLLSDLVTRMVIPLMSKD
                     KFGKGIIKNVNPVITIKKKEYVLITQQMAGIPNSQIGSSVCDCLEERHEILSA"
BASE COUNT          579 a          382 c          433 g          498 t
ORIGIN      
        1 tgtatgaagg tttttcgaag atacgcactc gcggtcttct gtctataaac gatatcatcg
       61 acgtgcagcg attgctcata cagaatgatt caggcatccg gaactcgatc ggtacggcgc
      121 tcgtcaacga tcgtacgcat gaagtgatgt atacaccgcc tgatgattat accgttatca
      181 atgctcttct caagaatttc gttaagtatc gcaatggtga cgggccgacg cttgcgatcg
      241 tcaaggacac gttgcccgga atctactcga aggagttggt cgaaactctt tttgtccatc
      301 catattgcaa gggcgagttt ttatacatac ggaacttatc cggctactga aggaatagat
      361 tgaacagtca ttttttctta tcgaattccg ctcacggtaa gcctcattct gcaaaacatc
      421 tacaaatcct tggtccagaa cttggtgatg gtttttattt tacacaatct tctatacatg
      481 ataacattac tcacattgat gcttccggga ggatgtgatg attgaagatg atctgcgcaa
      541 cggacacatg accaacgaga tttgggagtc ctggctggcg aagctggatg agacgaacaa
      601 acttttgatc gaacgggagt ggattgaaga gcctttctcg aaggagacga agttctataa
      661 catacgtccg ggctgcacgg acttcgagat caagaggatc caggaaacca acttaatcag
      721 acttcctgat gatttcatcg aagtggccac gcgttattcc gcgggcttag aaatcgctcg
      781 atgggaatgt ccggtaaaag acttgcccgc cgaatacaac gggagctacc taaaatacca
      841 tctatggcgc cttaatggat atttttcttc cgatcccctc tgggagattc ctgatagtaa
      901 agacctcagg tggggagggc ctcatgacac aggctggagc acgatagagg attttggaga
      961 agggcacgcc gagtacgttc gcatctggtc gaaaagaggc tactgcctga acaacctggg
     1021 aaacggcgac atgattgcta tcgacctgtc ctgggaaacc gaagcggctc ctgttttata
     1081 tttttcccat gacgatccgg acctgcacgg catacgattg ggatacagct ttatcgatta
     1141 catcgacaga ataatcagct tctgctgcgc cgaccccgaa cagatcgcga aggaggcgta
     1201 tgatagtaag caacatataa tagacaccga caagatcctg aaagagtgga agcgacagct
     1261 gctggatgtg aaataagggg gtcgggtttg tttacgccaa tttcttgaca aatataatac
     1321 atgcgtatat gttatgtgta tgcgggagat gtattatgtc aactttgtat aatgttaatg
     1381 cccctaaaag accgaccaac ctaacagtta acagtgattt attgactcag gcaaaagact
     1441 acaagataaa tgtatcagca actcttgaaa ctgctttagc agaagctctt aaatcaaaaa
     1501 aagtggacct ttggaaggaa gaaaacagag aagcaataca atcatataat attattcttg
     1561 aagaatatgg tctttttagc gatgagatca ggatgtttta atgtctcaat atcatgtgta
     1621 tgaaaatttg aatgagaaat caaaaaaact atatccatat gtaattgatg ttcaatctac
     1681 attattgtca gacctggtaa ccagaatggt tatcccattg atgtctaaag ataaatttgg
     1741 gaaagggata atcaaaaatg taaatccggt aataacgatt aagaaaaaag agtatgtact
     1801 cataacacaa caaatggcgg gaattccaaa ctcacaaatt ggatcatcag tttgcgattg
     1861 cctggaagaa cgccacgaga ttctttcagc aa
//
LOCUS       DAOQXQ010000373         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72334_length_1892_cov_2.329341, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000373 DAOQXQ010000000
VERSION     DAOQXQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72334_length_1892_cov_2.329341"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKH81_06510"
     CDS             <1..446
                     /locus_tag="PKH81_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97724.1"
                     /translation="TPIGMLKRAHHFPRLILNSAEAYTTDTAYRIHTTTPTVSAEMLV
                     YSFINSLTALTAELQGRHYGGGVLEMVPSEIEKLSIPLGKMDAIDLRELDRQIRQSIS
                     ADSILAAQDAIVLKAAGLTKRERETIHEAWRRIRSRRHRSNSEEG"
     gene            449..>1892
                     /locus_tag="PKH81_06515"
     CDS             449..>1892
                     /locus_tag="PKH81_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97725.1"
                     /translation="MRPSKQVANLVSRFLADQARYCSGTSGYTETEVRVEFIDPLFAI
                     IGWDMANSGGLPNSLKDVVREESQSTETSTKRPDYTFRVASIRKFFLEAKKPSVDIRT
                     NRDSAFQIRSYGWTAGLSVSILTNFRILRVYDTRSAPNEADHADTGLLLEIMCEEFET
                     RINEIHSLFGREAVATGSLETNFRTSDTGGIPIGKVFLDRFNSWRLKIATDLCARYPS
                     LGLAELNDLSQKIINRVIFVRMCEDRGIEGEGALRKVAEKRNIVELRRFFKEMDNRYN
                     TGLFDVAADPLQSKYALSSAVFLEIVEELYFPKAPYSFSVLDADFLGQVYEHFLSKQL
                     MSNGAGEITLNDKPAYEGREIVTTPQPIVDELVRRTFSGRLSVGIANLEVLKALRVLD
                     VAVGSSRFLLRALDELIDAAITCFRRTGDTSNIYRRSENDYRLRFEIKRDILLACLYG
                     IDIDYNAVEVARFSLLVKLLEDENRGTLPTG"
BASE COUNT          531 a          432 c          452 g          477 t
ORIGIN      
        1 caacaccgat cggaatgctg aaacgggcac accatttccc aaggctcata cttaattcgg
       61 cggaggccta cactaccgat accgcatacc gaatccatac gactacgcct acggtgtcgg
      121 cagaaatgct tgtttactcg ttcattaata gtttgactgc actgacagca gaactccaag
      181 ggaggcacta tggcggtggg gtcttggaaa tggtcccgtc agaaatcgaa aaactttcca
      241 tcccccttgg gaaaatggat gcaatcgatc tacgcgaact tgatcggcaa atcaggcaaa
      301 gcatatcagc ggactctatc cttgctgccc aggatgccat agtgcttaag gctgcagggc
      361 tgactaaaag agaaagggaa actatccacg aagcttggcg acgaatccga tccagaagac
      421 accgaagcaa ttcagaagaa ggataattat gagaccatcc aagcaagtag caaatcttgt
      481 atcaaggttt ctcgccgacc aggcacgtta ttgttcgggt acatcaggat atactgaaac
      541 tgaagttcgg gtcgaattta ttgacccttt attcgcaata atcggttggg atatggctaa
      601 ttctggaggg ctaccaaata gtcttaaaga cgttgtacgc gaggaatcgc aatctacaga
      661 aacatcaaca aagcgccccg actatacttt ccgtgttgca tctatacgga aattctttct
      721 tgaggcaaaa aaaccatcgg tcgatatccg cactaaccgt gatagcgcat tccaaatcag
      781 aagttacggg tggaccgccg ggctctccgt atccatactg actaatttca gaatactgcg
      841 cgtgtatgat acgcgttcgg ccccgaacga agcagaccat gccgacaccg gcctactgtt
      901 ggaaatcatg tgcgaggaat tcgaaacacg gattaacgag atacattctc tatttggccg
      961 tgaagccgtc gcgacaggaa gcttggagac taattttagg acatcagata ctggtgggat
     1021 acctattggt aaagtgttcc tcgatcggtt taatagttgg cgcctcaaga ttgctacaga
     1081 tctctgtgcc agatatcctt cccttggcct tgctgaatta aacgatttgt ctcaaaaaat
     1141 cattaaccgg gttatctttg tccggatgtg cgaggatcgc ggcattgaag gagaaggggc
     1201 tctacgaaaa gtcgctgaga aacgcaatat tgttgagctt cgtcgattct tcaaggaaat
     1261 ggacaaccgt tataacacgg ggcttttcga tgtggccgcc gatccacttc aatctaaata
     1321 cgctttatct tctgccgttt ttcttgaaat agttgaggaa ctctacttcc caaaagctcc
     1381 atacagcttt agtgtcctgg atgccgattt cctcggtcag gtctatgaac actttctgtc
     1441 aaagcaactt atgtccaacg gagccggaga aatcacactt aatgacaaac cggcatatga
     1501 ggggcgagag atagtaacga cgccacaacc aattgttgac gagcttgtac gcagaacatt
     1561 ctcgggacgg ctctctgtag ggatagctaa cctcgaagta ctgaaggcat tgcgtgttct
     1621 tgatgtggct gtagggtcaa gccgctttct cctccgagca ttagacgaat tgattgacgc
     1681 cgcgataacc tgtttcagaa gaactggtga tacaagtaac atctatagga gatcagaaaa
     1741 cgattatcga cttcgcttcg aaataaagcg agatattctt ttggcatgct tatatgggat
     1801 cgatatagac tataacgctg ttgaggtagc tcgtttcagt cttctggtga agcttcttga
     1861 ggatgaaaat agagggacat tgcctaccgg ga
//
LOCUS       DAOQXQ010000374         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72694_length_1886_cov_2.878209, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000374 DAOQXQ010000000
VERSION     DAOQXQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72694_length_1886_cov_2.878209"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..227)
                     /locus_tag="PKH81_06520"
     CDS             complement(36..227)
                     /locus_tag="PKH81_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97726.1"
                     /translation="MKKYLHIIVPCIAVFLCSLFLFTTLDQKVFDLFLRTIPSLKENA
                     SVVVVKIDDNSIERVGIFP"
     gene            complement(270..1616)
                     /gene="murD"
                     /locus_tag="PKH81_06525"
     CDS             complement(270..1616)
                     /gene="murD"
                     /locus_tag="PKH81_06525"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ97727.1"
                     /translation="MGLGLNGGGLATARFMASHGALVTVTDRKTAVELQPSIDALSAY
                     PSIRYVLGTHELEDFSTADVVIKNPGVKLEGNQYLDVAPAIESDISIFLRLTKAPIIA
                     VTGSKGKSSTVSALQFALRESGLPSFLGGNITVSPLTFLEETSPHTPTVLELSSWQLA
                     DLRGLGLLDPRVAILTPIMSDHQNWYNGMESYVADKKLIYADQSDSGYLLCNKDDGWG
                     DIFARECKSRPLWYSATRLADGLEGIWLEGDGQGMMRFEGTERVILSKDLAVPGFHMR
                     QNLLNAAGALALFGLKSDTTALALSRFPGIEHRLEHFHQEKGVRWFNDSAATIPEAAA
                     AALSSFNEPVVLLCGGTDKNLDFAPLASVAAKAKKIILLAGTGTDKLIPLLTQKGIDF
                     IGPCADLASLIASARREATSGDVVVFSPGATSFGLFLNEFDRGRKFKENVVSSCSA"
     gene            complement(1669..>1886)
                     /locus_tag="PKH81_06530"
     CDS             complement(1669..>1886)
                     /locus_tag="PKH81_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97728.1"
                     /translation="SPEPLHRIREFIEPRIPLILRDLQMVSFSPTMGLFTDMKKEIPE
                     ALGVLWNNLTVRAYLNELNQKEYSIEL"
BASE COUNT          470 a          454 c          514 g          448 t
ORIGIN      
        1 caagaagact atggcgtctg ccataatatc gcgtgtcagg gaaagatgcc gacgcgttct
       61 attgagttgt cgtctatctt gacgactacc acggatgcgt tttctttaag ggacgggata
      121 gtccgcaaaa agagatcaaa gaccttttga tcaagggtgg taaacagaaa gagcgagcac
      181 agaaaaacag caatgcaggg gacaataata tgcaggtact ttttcatgct accagtatat
      241 accgcctcgc cgtcagcgca aggagaaaat catgctgagc aagaacttac tacgttttct
      301 ttgaattttc tgccgcggtc aaactcatta agaaaaagac caaagctcgt cgctccgggg
      361 gagaacacca ccacatcccc ggacgtagcc tcccggcggg cactggcgat aagggaagca
      421 agatctgcgc acggtccaat aaagtcgatg cctttttggg taagcagggg gatcagcttg
      481 tctgtgccgg taccggcaag aagaataatc ttttttgcct tggcggcaac cgaagcaagc
      541 ggggcaaaat caaggttttt atcggttccc ccgcagagca ggacgaccgg ttcgttaaaa
      601 gaagaaaggg ctgccgctgc ggcttcgggt atggttgcgg cggagtcgtt aaaccagcgc
      661 actccttttt cctggtggaa gtgctccagt cgatgttcga tgcccggaaa tcgggacagg
      721 gccagggcgg tggtatcgct tttcaagcca aacaaggcaa gggctccagc ggcgtttaac
      781 aggttttgcc tcatgtggaa accggggact gcaaggtcct tggacagtat gacgcgctcg
      841 gtgccttcaa agcgcatcat tccctgtccg tccccttcca gccagatacc ctcaaggcca
      901 tctgcaagcc gggtggcgga ataccagagg gggcggctct tgcattcgcg ggcgaatatg
      961 tctccccatc catcatcctt gttgcataat agatagccgc tatcgctttg atcggcgtat
     1021 atcagctttt tatccgctac atagctttcc attccgttat accagttttg atggtcgctc
     1081 atgatgggcg taaggatcgc tacccgggga tctaaaagac ccagaccgcg caggtcggct
     1141 aattgccagc tggaaagctc aagaacggtt ggggtatggg gcgaggtttc ttcaagaaag
     1201 gtcagggggc ttacggtaat gtttccgcca agaaaggacg gaaggcccga ttcgcgaagc
     1261 gcaaactgaa gcgcgcttac tgtagaggat tttcccttgc tgccggttac cgctatgatg
     1321 ggcgccttgg tcaggcgaag aaagatggag atatcggatt ctatagccgg tgctacgtca
     1381 aggtattggt tcccttcaag ttttactcct ggattcttta ttacaacatc agccgtacta
     1441 aaatcttcca gttcgtgggt gccaagtaca tagcgaatgg aaggatacgc gcttagcgca
     1501 tcgatggaag gctgtaactc cacggcagtt ttacggtcgg ttacggtaac caatgcccca
     1561 tggctcgcca taaaacgagc ggtggcaagg cctccgccgt taagccctaa tcccatgacg
     1621 gtaacacgga gaccggcaat atcggaaatt gatgctagtg gatagggttt acagttcaat
     1681 ggagtactcc ttttggttta gttcgttaag ataggcgcgg actgtcaaat tattccatag
     1741 aacgccaagt gcttcgggta tttctttttt catgtcggta aacagcccca tcgttggcga
     1801 aaaacttacc atttgtaaat cccgtaaaat aagcgggatt cgcggttcaa taaactcccg
     1861 tatccggtga agcggttcag gagagc
//
LOCUS       DAOQXQ010000375         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72698_length_1886_cov_2.701256, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000375 DAOQXQ010000000
VERSION     DAOQXQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72698_length_1886_cov_2.701256"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKH81_06535"
     CDS             complement(<1..1080)
                     /locus_tag="PKH81_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNQ97729.1"
                     /translation="MMVLKSRFIIVAVSFLVVAGFSVHAQQKNALLIANARYSHFASL
                     ATPVAEARELKGSLESIGFTVTLLEDASREAMMDAILDFEEILEKQGGIAFFHYGGHG
                     IQVNGKNYLIPSNAEIPDERRVETRSVSVDEIMASLDAAATNANIVVLDACRNNPLPA
                     TSTRSTGRGLSVVAVRPRNSILVYSAEAGNIAQDGLFTPALCSAIRQEGKSFQDILME
                     VRKQVSEKTGGAQVPGEYNQSFEPIYLAGLPAQQTPGNAEEPASPARPPVAAVSPVKT
                     HQSPAMASIDGGTFYIGRARHHKVTLSPYYIGVTEVTQAEYESVIGSNPSAFQDPSRP
                     VECVSWYDAIRYCNALSEREGLTPAY"
     gene            1274..1537
                     /locus_tag="PKH81_06540"
     CDS             1274..1537
                     /locus_tag="PKH81_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97730.1"
                     /translation="MKSIKTKISGMAIGITIAVTVVLLTVFFLSFSTMVNEQMTLLDT
                     TLRESFDRTMRWEVESAEELAASAESLSAQAETLRDTVSRFKV"
     gene            1563..>1886
                     /locus_tag="PKH81_06545"
     CDS             1563..>1886
                     /locus_tag="PKH81_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97731.1"
                     /translation="MIRCFAVLILATMLFGCQENKNNFTYNPQSLPNEYRINLVRIPQ
                     NDNYSCATTSLATIINFYKNPPNPIDKNDIWELSETKIEDVRTRGNDVKGLERIAEHY
                     NYAYSF"
BASE COUNT          513 a          455 c          424 g          494 t
ORIGIN      
        1 atacgcgggg gtgagtccct cccgttcgct cagcgcgtta cagtaacgta tcgcatcgta
       61 ccaggagacg cattcaaccg gccttgaagg gtcctgaaat gcgctcggat tgctcccgat
      121 taccgactcg tactcggcct gggtaacttc ggtaacccca atgtaataag gacttaaagt
      181 aaccttgtga tgtcttgccc ttcctatata aaaggtgccc ccatcgatag aagccatagc
      241 cgggctttgg tgtgtcttga ccggggatac agcagccaca ggaggccttg caggggatgc
      301 gggttcttct gcattgccag gcgtctgctg cgcggggagc cctgcaagat agataggctc
      361 aaaggattgg ttatattcac cgggaacctg cgctcctccg gttttttcgg aaacttgttt
      421 tcggacttcc atcaggatat cctgaaaact tttaccctcc tgacggatcg ccgaacaaag
      481 ggccggggta aacagaccgt cctgcgcaat attgcccgct tcggccgaat agaccagaat
      541 cgaattccgc gggcgcaccg ccacaacgga aaggccgcgt cctgtactac gggtgcttgt
      601 agccggtaga ggattattcc ggcaggcatc cagaacaacg atattggcat ttgttgcagc
      661 ggcgtcaaga cttgccataa tctcgtccac agagacagac ctggtctcga ccctgcgttc
      721 gtccggaatc tcggcattag atggaattag gtaattcttg ccgtttacct ggatcccatg
      781 cccgccataa tggaaaaagg cgattccgcc ctgtttttca agtatttctt caaagtccag
      841 aatcgcatcc atcatggctt cgcgacttgc gtcttcaaga agggttaccg taaatccaat
      901 tgactcaagc gatcccttca gttctctcgc ctccgctaca ggagttgcaa gactcgcaaa
      961 gtgactatac cgcgcgttag caataagaag agcgtttttc tgttgggcat gtaccgaaaa
     1021 cccggctacc actaaaaaac tgactgccac aatgatgaat ctggacttta gtaccatcat
     1081 aaatccccat gtaaggtgtt attgatctgt atcgtataca atataacatt ttagtcagtt
     1141 aatcatgtcc ctgacatgaa gtaaagaaat atctactggc aaatgttctc ctcctttcat
     1201 ttttggcata ttagtccgta atctactacg ttttatacat cctgaattga tatagccttt
     1261 tgcggagggt ctgatgaaaa gtattaaaac aaaaattagt ggaatggcta ttggcattac
     1321 tattgcggta acggtagtgc ttttaacggt gttttttttg tcttttagta caatggtgaa
     1381 cgagcaaatg acattgctgg ataccacgct tcgcgaaagc tttgaccgaa ctatgcggtg
     1441 ggaggttgaa tccgctgagg aactagccgc ctcggccgaa agcctaagcg ctcaagcaga
     1501 aacacttcgt gacactgtgt ccagattcaa agtataatag ccgcaagaca agaggtttcc
     1561 acatgatcag gtgttttgca gtactcattt tggcaaccat gctattcggt tgccaagaaa
     1621 ataaaaataa ttttacctat aatccacaat ccttaccaaa tgaataccgg ataaacttgg
     1681 tacgcatccc acaaaatgac aattactcat gtgcaaccac atctttggca actattatta
     1741 atttctacaa aaacccgcca aacccaatag acaagaatga tatctgggaa ttatcggaaa
     1801 caaaaattga ggatgttcgc acacgcggaa atgatgttaa aggtttagaa cgaattgcag
     1861 agcactacaa ctacgcctat tccttt
//
LOCUS       DAOQXQ010000376         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_72816_length_1884_cov_3.142154, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000376 DAOQXQ010000000
VERSION     DAOQXQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72816_length_1884_cov_3.142154"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKH81_06550"
     CDS             complement(<1..441)
                     /locus_tag="PKH81_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ97732.1"
                     /translation="MHHRKADAKTRSEESVGGRIDAVLGRIQKRVLTGASLEEIIDSI
                     FREIRRIVPCDRIDIAFIEEGGSRLLLHHTRARYSPIHLTPGFASDIVGSAVHAIFLK
                     GKNRIIGDFAYYLRKYPKSESARLLLKEGIHSSMACPFYAGKKPI"
     gene            663..1253
                     /locus_tag="PKH81_06555"
     CDS             663..1253
                     /locus_tag="PKH81_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ97733.1"
                     /translation="MPRPKRIIAPGFTYHVFCRCIEKRALLQTTFASNLLVKVLAQTQ
                     EKYNYDLIAYQIMDNHFHFVIQTRENEASISTIMQYIKARFAEKYNRAKGRSGPFWNE
                     RFGDTIVEKSDRPVIYLLYLLWYLAFNPVRKSLASNPRLYPYGSIKSYLEENYESPVR
                     ISLHEYFLDLGKTFAERVKAFLYYEEAYRKRLALLF"
BASE COUNT          485 a          496 c          409 g          494 t
ORIGIN      
        1 gatcggcttc ttgcccgcat aaaacggaca ggccatgctt gaatgaatcc cctctttaag
       61 aagcagtctg gcggattcgc tcttgggata tttccgcaga taataagcaa aatccccgat
      121 aattcggttc ttccctttga ggaatatcgc gtggactgcg ctcccaacta tatccgaggc
      181 aaaaccgggc gtcagatgga tgggcgagta gcgggcgcgc gtgtgatgta atagcagccg
      241 gctccctccc tcctcgataa aggctatgtc gatacggtca catggaacaa tacgacgaat
      301 ctccctgaag atcgaatcga taatctcctc gagagaagcc cctgtgagga cacgcttctg
      361 tatccgcccg agtaccgcgt caatccggcc tccaacactt tcctcggacc gggtctttgc
      421 atccgcttta cgatggtgca cctggccccc tccctcattg gaaacgaaaa ttgatcgagc
      481 atatcgacat ttttgtataa ttgtcaagaa gaaagggaag aaaggggtca gagccctcct
      541 gcgacaaaaa tagggggtca gagcctatcc caaacttttt tctcatacct tcgaaaaact
      601 tttaactttc atccatcgaa aaacgtttta ccaacagaag acaatggatc agggaggttt
      661 ttatgccgcg gccgaaacga ataatcgctc caggcttcac atatcatgtg ttttgccgct
      721 gtatcgaaaa gagggcactg cttcaaacta cattcgcgtc gaatcttctc gtaaaggtac
      781 tggcccaaac gcaggagaaa tataactacg acctcatcgc ctatcagatc atggacaatc
      841 actttcactt tgtcattcaa acaagggaaa atgaggcgtc aatttccaca ataatgcaat
      901 acatcaaggc gagattcgcc gaaaagtaca atcgcgccaa aggaagaagc ggcccatttt
      961 ggaacgagcg attcggggac accattgtag agaaatccga caggccggta atatatctgc
     1021 tataccttct ctggtatctg gccttcaatc cggtgagaaa aagcctggcg tcgaatccgc
     1081 gtttataccc ttacggcagc ataaaaagct atctggaaga aaattacgaa tcgccggtac
     1141 gaatttcgtt acatgaatat tttctcgatc ttggtaaaac ctttgcggag agggtaaagg
     1201 cattccttta ctacgaggaa gcctatagaa agcgcctggc cctgcttttc taagacggtg
     1261 cattcaattc attgttcatc cggcctgatc ggaatacgat cggggaggat ccgccttccc
     1321 gcaaaccctc cgattcaccg aaaactctca acagtttatc gcatttcatt gaaatccgca
     1381 gcccttttta ctcatatcgc taactgacga tgcacagcag gcgattgggt tgatataatc
     1441 acgcttggtt ggcggaaatg gagactgaaa atgaagatgg gtcagagcct gaagcacaag
     1501 gggtcagagc cctcccaccc caccaacaaa agagcgcggc ttccgccgcg ctcttttgtt
     1561 gcctctacgc attttctacg ctttttctga ccttttactt ctcttagcct tctggcatag
     1621 ctttacttat taataacctt agccttcagg catcacttta ccttttgtgt cctttgcctt
     1681 aatgcttctc tttgccttca ggcttcccct tcgacttaag gctctgtttt acccttcaag
     1741 cctcccttgg ccttaagcac aaggggtcag agcctctctc ctcgaaatta gaacttaact
     1801 tccgacttta tccagaccat gtcttcttta atcttggact taacgccggt attgctggtg
     1861 gtttcccgct ctccggtctg gtac
//
LOCUS       DAOQXQ010000377         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73169_length_1879_cov_2.884320, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000377 DAOQXQ010000000
VERSION     DAOQXQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73169_length_1879_cov_2.884320"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1153)
                     /locus_tag="PKH81_06560"
     CDS             complement(110..1153)
                     /locus_tag="PKH81_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ97734.1"
                     /translation="MNIEYTSRLEKIESVLSSRLPESIGTGFMSDAFSDLPHAVRPLH
                     LASLNDPCRDLMLRGGKRWRPLLMLLSCELAGGGTRALQLTPIVELVHTASLIHDDIE
                     DHADERRGKPAIHLIYGEDTAINCASWLYFYAQSIIDSFPSSDSLKLILYKTVSRELR
                     RLHLGQAMDIRWHRDPSLVPVRSEYEAMISLKTGTLSRLAAELGMLAAGCDPADATSL
                     GDLAAKIGVGFQVLDDVRNLTTGNPGKRRGDDIVEGKKSLPVIMHLEKHGNDLAELSA
                     CFTQAREEGIQSPAVEKAISMLESSGVIPASRDWGSSLIGNACLTLRGRYPDNPAAEL
                     ISGLFESMLADLP"
     gene            1198..1794
                     /locus_tag="PKH81_06565"
     CDS             1198..1794
                     /locus_tag="PKH81_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNQ97735.1"
                     /translation="MSSNSYEKPDFWSKKAFSEGYPARSVYKLKEMDEKFTLIKPNTR
                     VLDLGAAPGSWTTYVLRVLAGSGHLTAIDLSPLAKDVKGDNLSFFQGDLYSPAIRKEA
                     LDRGPYNLVICDAAPATTGNRTVDTARSSGLVELAIYYAETMLVSGGNFVVKIFQGGG
                     EQAHLLAMRKLFTSARGFKPEACRSGSFETYLIGLGKK"
BASE COUNT          516 a          485 c          453 g          425 t
ORIGIN      
        1 acaccgcatt accatcgtcg gtttgggaaa tagtccaaat ttgcgcgggc aaaaaacgtg
       61 cagtcctcat actataagaa tactacaccg agggacggaa aacaataaat tagggaagat
      121 cggcaagcat agactcaaaa agtccggaaa tgagctcggc agcaggattg tcaggataac
      181 gccccctcag ggttaagcag gcgtttccaa tcaaggaact gccccaatct cgtgatgcgg
      241 gaataacacc ggacgattca agcatactga tagccttctc cactgcaggg gattgtattc
      301 cttcttccct tgcttgggta aaacaggcag aaagttcggc cagatcgttt ccgtgttttt
      361 ccagatgcat gataaccggc aaactctttt tcccttccac aatgtcatca ccgcgtcttt
      421 tgccgggatt tcctgtagta aggtttcgca cgtcatccaa aacctgaaaa cccacgccaa
      481 ttttggcagc aagatcccca agggatgttg catcggctgg atcacagccg gcggcaagca
      541 tgccaagttc agcagccaaa cgggagagag ttcccgtctt taagctaatc atcgcttcat
      601 attcagatct aaccggtacc aaggaaggat cccgatgcca tcgaatatcc atcgcctgac
      661 caaggtgcaa ccggcgcaat tcgcgagata cggtcttgta taaaatcaac ttaagggaat
      721 ctgaagacgg aaaggaatca atgatggact gggcataaaa atacagccag gaggcgcagt
      781 taatggccgt atcctcccca taaatcagat ggattgcggg tttcccgcga cgctcatccg
      841 catgatcttc tatatcatca tgaatcaaac ttgcggtatg gaccaattca acaataggcg
      901 ttagctgaag ggcccgcgtg cctccgccgg ccaattcgca ggaaagaagc atcaacaggg
      961 gtctccagcg tttacctcct ctgagcatca agtccctgca cggatcgtta agggatgcca
     1021 ggtgaagcgg ccgaactgca tggggtaagt cggaaaacgc atcgctcata aaacctgtcc
     1081 cgattgattc aggcaggcga gaagataaca cactctcgat cttttcaaga cgactcgtat
     1141 attctatatt catacccggt attgtatccg atccatcagt tcttgcaagg ggagtccatg
     1201 agctcaaaca gttacgaaaa acctgacttc tggagtaaaa aagccttttc cgaagggtat
     1261 cccgccagat cagtgtacaa gctcaaggaa atggacgaga aatttactct gattaaaccg
     1321 aatacccgag ttctggattt gggggccgcg ccgggaagct ggaccaccta tgtgctgcgg
     1381 gtactcgccg gctcaggaca cctgacagcc attgacctgt ccccgctggc caaggatgtt
     1441 aaaggggata acctttcttt ttttcaggga gacctgtata gcccggcaat ccgcaaagaa
     1501 gccctggaca gaggccccta taatctggtt atctgcgacg ccgcgcccgc tacaaccggc
     1561 aacagaaccg tagacaccgc acgatcaagt ggtcttgttg aactggctat atattacgcc
     1621 gaaacgatgc tcgtgtccgg gggcaatttt gttgttaaaa tctttcaagg cggaggagag
     1681 caagctcatc ttctggccat gagaaagctc tttacctccg cacgaggctt taagccggaa
     1741 gcttgccgct cgggcagctt tgaaacctat ctaatcgggc tggggaaaaa atgaacaggg
     1801 gatcttttct ctgcatttcc tccacattta cgccgaaaat ttccacaaac ccatgcacac
     1861 aatggtatac tggagcaat
//
LOCUS       DAOQXQ010000378         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73183_length_1879_cov_2.400768, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000378 DAOQXQ010000000
VERSION     DAOQXQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73183_length_1879_cov_2.400768"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PKH81_06570"
     CDS             complement(<1..1328)
                     /locus_tag="PKH81_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNQ97736.1"
                     /translation="MKHLAMLILLLTVAIPLYAFSLSDSAEALISENAGLTFSVFFGQ
                     VEGPDEHIIIRDAHNEYHLYHLRNENIVRVVKLPPIFKEISRMETQADGYLLHGNIGY
                     ELVSSITDSEVIVKINHYGELRSYYFPQAKIAPVSPIMQGKQKNGRFLFEWFAVSSEP
                     FVFRILAGPSKNQLRILRENLYLNYIEIKGEDLKGMPQVFWKIEGIRNDGTIVESPLA
                     SFYTGFEPSIGKTSETGIEGVVRDAETLLPLEKASVILLDQKTRIREEVATDESGFFS
                     FNIAPGKDYQLTVYKKGYLEACYESIEVVSQRNTFLEPILQIDEERSGAGVLRGRIVE
                     ATVKDQGIPNVSVWVRQGINQSEGEPVFQSVSLSDGSFVLEGVPVGNYTLQFQAEGYI
                     DNHAQMVSLGETHVTELTVTMARTPDNVESVRIVLSWGTEGLDLDAHLTGP"
     gene            complement(1374..>1879)
                     /locus_tag="PKH81_06575"
     CDS             complement(1374..>1879)
                     /locus_tag="PKH81_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ97737.1"
                     /translation="GNITTDIKQVEGYRLPTEAEWEFAAKGGNSEGGNYQYAGSDDLD
                     DVGWYWKNSGDSKLPGDDSNWDWPMIVRNNSRTHETGQKDPNGLGIHDLSGNVFEWCH
                     DWFGLYTADTYHNPIGTKTRSDFKVTRGGGWSYIGLYNRIENRSYGNPVTKAATVGFR
                     IARTKIE"
BASE COUNT          452 a          485 c          397 g          545 t
ORIGIN      
        1 ggcccggtta aatgagcgtc caaatcgagg ccctccgtgc cccaggacag aacgatccgg
       61 acgctttcaa cgttatcggg cgttcgcgcc attgtgacgg tcaactcggt tacatgggtt
      121 tcgccgagcg aaaccatttg ggcgtgattg tcgatgtatc cttccgcttg aaattgcaag
      181 gtatagttgc cgaccggtac accttccaat acgaacgacc cgtctgagag acttacgctc
      241 tggaagacag gctccccctc gctttggtta attccttgcc ttacccatac gcttacgttt
      301 ggaatgcctt gatctttgac ggttgcttcg actattctcc ctctcaagac cccagcgcct
      361 gatctttcct catcgatctg gaggatgggc tccaaaaacg tgtttctttg cgagacaacc
      421 tcaatcgact cgtagcaggc ttccaagtat ccctttttat agacggtcag ttgatagtct
      481 ttacccggcg cgatgttgaa agaaaaaaag ccggattcgt cagtagcgac ttcctcgcgt
      541 attctcgttt tttgatcaag aagaatgact gaagcctttt caagaggcag gagcgtttcc
      601 gcgtctctga cgaccccctc tatgcccgtt tcactcgttt tccctataga gggctcgaat
      661 ccggtataaa aagatgccaa cggactttct acgatagttc catcattgcg tataccttca
      721 attttccaga atacttgagg catccctttt aagtcttccc cttttatctc gatgtagtta
      781 agatacaagt tttccctgag gatccgcagt tggtttttcg acggtcccgc aagaattcta
      841 aaaacaaagg gttcagagga tacagcgaac cattcaaaaa ggaatcgtcc gtttttctgt
      901 ttcccttgca tgatcggaga aactggcgca atcttcgcct gagggaagta ataagaacgc
      961 aattcgccat agtgattaat cttgactatc acttcactgt cagtaatcga agaaaccaac
     1021 tcataaccaa tattcccgtg taatagatac ccatcggctt gagtttccat tcgactgatc
     1081 tctttgaaga tcggtggcaa tttgacgacg cgaacgatgt tttcgttcct aagatggtat
     1141 aaatggtact cattatgagc atctcttatg atgatgtgct cgtctgggcc ttcaacctgt
     1201 ccaaaaaaaa cggagaacgt taatccggcg ttttcactaa taagggcttc cgcgctgtcg
     1261 gaaagtgaaa aagcgtacaa gggaatcgcg accgtcaata aaagtatgag catagccagg
     1321 tgcttcacaa tatctcctcc tcataatagt tacgagtaat tttctataaa gcgctattca
     1381 attttagttc gagcgatccg aaatccgaca gtagctgctt tcgtaactgg gtttccgtaa
     1441 ctccgatttt cgatacggtt gtagagtccg atataagacc atcctccgcc tcgcgtaact
     1501 ttaaaatcgc ttctggtttt tgtcccgata ggattgtgat aggtgtcggc ggtatataag
     1561 ccgaaccaat catgacacca ttcaaagaca ttgccgctta aatcgtgaat gcccaatccg
     1621 tttgggtctt tttggcctgt ctcgtgcgta cggctgttgt ttctgactat catcggccaa
     1681 tcccaattag aatcatctcc aggcaatttc gaatctcccg aattcttcca ataccacccg
     1741 acatcgtcta aatcatcgct tcctgcatac tgatagtttc ctccttcgct gttccctcct
     1801 ttcgccgcga attcccattc cgcctccgtc ggcaatcggt atccttctac ctgcttgatg
     1861 tccgtcgtga tgttcccgt
//
LOCUS       DAOQXQ010000379         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73511_length_1874_cov_2.350742, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000379 DAOQXQ010000000
VERSION     DAOQXQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73511_length_1874_cov_2.350742"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKH81_06580"
     CDS             <1..1140
                     /locus_tag="PKH81_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNQ97738.1"
                     /translation="ISTVVAQADGEKAVRIAHRFFFNTMQTIELFLVGIGTVGGKLLD
                     QIKLQQQSLRESNIDLQVWSVANSRKMILKRGGLDLSSWKNCLEEATEESSIDGMISL
                     VKAEKPLNPVFVDCTATGAVPERYPDLFRSGFHVVTPNKRANSHTIDFYRQLRTLAQE
                     SRKRFLYETNVGAGLPVIDTFKNMLKSGDALLSFSGILSGSLSYIFGRLDEGVPFSVA
                     VGEARDKGFTEPDPRDDLSGSDVARKALIIAREAGITAELSSVTTRPLFPDDFDLSGT
                     TDDFMSRLPSVDSWFSSRIESLRNQGQVLRFVADVEGATGKIVVGVLSVSHTDPLAAI
                     KGGENAFVFTTRHYSPIPLVIRGYGAGADVTASGVFADILRTITW"
     gene            1134..>1874
                     /gene="aroF"
                     /locus_tag="PKH81_06585"
     CDS             1134..>1874
                     /gene="aroF"
                     /locus_tag="PKH81_06585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNQ97739.1"
                     /translation="MVIVLEKGIRDDDKQRIRTFLEKNSLRVNEIAGDEETIFGAVGK
                     VGIDPREVEILPGVQRVIPISKPYKMASREFKRENSVVNVRGVKIGGSRVSVIAGPCA
                     VESREQMLESAARVAESGAVMLRGGAYKPRTSPYAFQGMGEEGVKILREAGDAWGLPV
                     VTEIVSSEHIPAMRDYVDVYQIGARNMQNFELLKKVGALAKPVILKRGLSATIEEWLM
                     AAEYLLASGTEDVILCERGIRTYEKATRN"
BASE COUNT          445 a          432 c          491 g          506 t
ORIGIN      
        1 attagtaccg ttgtcgcaca ggcagacggg gaaaaggcgg tccgtattgc ccatcgcttt
       61 ttctttaata ccatgcaaac cattgaactt tttctcgttg gtattggtac cgtgggcggc
      121 aagcttctag atcaaattaa attgcagcaa caatcgctgc gcgaaagcaa tatcgattta
      181 caggtttggt cggtagccaa ttcccgcaag atgatcctta agcggggagg ccttgatctc
      241 tcttcctgga agaactgtct cgaggaagca accgaggaat cctctatcga cgggatgatt
      301 tctttggtta aagctgaaaa gccacttaat cctgtttttg tggattgtac cgcaacgggc
      361 gccgtgcctg aacggtatcc cgatctcttt cgttcgggct ttcatgtggt aaccccaaat
      421 aaacgtgcga attcccacac aatcgatttt taccgacagc tgagaactct tgcgcaagaa
      481 agcagaaagc ggtttttgta tgaaacaaac gttggcgcag gcctacctgt tatcgatacc
      541 ttcaagaata tgctcaagtc gggagacgcc cttctttctt tttccggtat tctctcaggc
      601 tcgctttcct atatttttgg ccggctcgac gagggtgttc ctttttcagt tgcggttggt
      661 gaagctcgcg ataaaggctt tactgaaccc gatccccgcg atgatttatc cggatctgat
      721 gttgcacgaa aggctcttat tattgcacgg gaagcgggga ttaccgctga gctttcttca
      781 gttactacgc gtcccctgtt cccggatgat tttgatcttt caggaacgac agatgatttt
      841 atgagtcgtc ttccttcagt ggactcctgg ttttcttctc gcattgaatc gttaagaaat
      901 cagggacagg tacttagatt tgtcgctgat gttgaaggtg ctaccggaaa gattgtcgtt
      961 ggtgtccttt cggtatcgca caccgatcct ctggcggcaa tcaagggagg cgaaaatgct
     1021 ttcgtcttta ccacgcgtca ttattcaccc ataccgctgg ttatacgcgg gtatggtgcc
     1081 ggtgcggatg ttaccgcctc cggtgtattc gcagatatat taaggacaat cacatggtaa
     1141 tcgtattgga aaaaggaatt cgcgacgacg acaaacagcg aatcaggacg tttttggaaa
     1201 agaactccct cagggttaac gagattgccg gggacgaaga gactatcttt ggcgctgtcg
     1261 gcaaggttgg catcgatccg cgcgaggtag aaattcttcc cggagttcaa cgggttattc
     1321 ccatatcaaa gccctataaa atggcatccc gagaatttaa acgcgaaaac tcggttgtaa
     1381 acgtccgtgg ggttaaaatt ggcggttctc gtgtttctgt tatcgccggt ccctgcgcgg
     1441 tcgaaagccg tgagcaaatg cttgagtccg cagcccgagt tgcggaatcg ggagcggtaa
     1501 tgctccgcgg tggtgcgtat aaaccgcgca ccagtcccta tgcttttcag ggtatgggcg
     1561 aggaaggagt caagattctt cgcgaagccg gtgacgcctg gggccttccg gtggttaccg
     1621 agattgtttc gagcgaacat attcccgcca tgagagatta tgtagatgtc tatcaaattg
     1681 gcgcgcgcaa tatgcaaaac tttgaattac tcaagaaagt tggagctctt gcaaagccgg
     1741 ttatccttaa acggggactt tcggccacga tagaagaatg gttaatggcc gccgaatatt
     1801 tattggcgag cggcaccgag gatgttatcc tgtgtgagcg aggcatacgt acatacgaga
     1861 aggctactcg aaac
//
LOCUS       DAOQXQ010000380         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73584_length_1873_cov_1.952695, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000380 DAOQXQ010000000
VERSION     DAOQXQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73584_length_1873_cov_1.952695"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..504)
                     /locus_tag="PKH81_06590"
     CDS             complement(40..504)
                     /locus_tag="PKH81_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNQ97740.1"
                     /translation="MIITLLVLGLLLIIAEMTVIPGFGITGILGLASFIGAIVMAFTQ
                     FGNTIGFIVLFAALLLCGVLLFFVLRSKTWRKITLHQKIDQKAIPSPQEKGIVVGMEG
                     VSLTRLNPAGNGRFGDVTIEVRSSQGLIQANTPIKVVYIDGLSIFVSPVENN"
     gene            complement(521..>1873)
                     /locus_tag="PKH81_06595"
     CDS             complement(521..>1873)
                     /locus_tag="PKH81_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97741.1"
                     /translation="SSDGKTLYFSSNGLPGLGGMNLYVSRWDTKTQEWGLAENLGIPF
                     SSTHNDLLYMLSDDSRYFYFTSDRAAPKDSLVLYKVEYESAPVKIRPASVKELREIAM
                     MPLSGNKTRDREYGPKEAGMGVPDSILEKTREATAHYPQLTAAVRNLKVQVAGHEKKL
                     DSLRKYYSSLTPEEDRRAIANTIREEEFVLMELQQSLRDTRKSAQSIEDLFLSHGVLP
                     PVTTPASKSPASSASGLQEMDLFPVKQQLISLDSHLFEAPLPVVPPVNLEFRIEQESV
                     IVPWENEPPGLYYRIQLFTVARKATISQLKGISPIFEVRAGNHYVYYAGQFYNYADAA
                     RALASVKRRGITGAIVVAYFQGKSIPVLEGRRREAQNKTAPEGVAAFHVYLGSNQIPP
                     ALISLVGELSDKDIIRVITGSGTDYFIGPFASASQAEELASVLREKGFDTVTVQQVTN
                     "
BASE COUNT          464 a          458 c          454 g          497 t
ORIGIN      
        1 acattaacgt tatacctaca cttgacataa tattttcttt tagttgtttt ctactggaga
       61 tacaaatata ctcaatccat cgatatagac gaccttgatg ggggtattag cctgaataag
      121 gccctgggaa gagcggactt ctatggttac atcgccaaac cggccgttgc ctgccgggtt
      181 caggcgggtc aggcttactc cttccatccc cacgacgata cctttttcct ggggggaagg
      241 gatggctttt tggtctatct tctgatgcag ggtgattttg cgccaggttt ttgaacgcag
      301 gacaaaaaac aacagaacac cacacagtag gagcgctgca aacaaaacga taaatccgat
      361 ggtgttcccg aattgagtga aagccattac gatagcgcca atgaagctgg ccagacccag
      421 gatgccggtg atgccaaatc cggggatcac agtcatctcg gctatgatta acaaaagtcc
      481 caggaccaaa agtgttatga tcaatcccat atctttatta ttaattggtt acttgttgaa
      541 cagtcacggt gtcaaatcct ttttcacgaa gcacagaagc cagttcttct gcctgggaag
      601 ccgaagcgaa agggccgatg aaataatcgg ttccactgcc ggtaattacc ctgattatat
      661 ccttgtccga gagttcaccc accagggaaa taagggcagg ggggatttga ttgcttccca
      721 ggtaaacatg gaaagccgcc actccttcgg gtgccgtctt gttttgggct tccctgcgcc
      781 ttccctccag gacaggaatg gattttccct gaaaataggc cactacaatg gccccagtga
      841 taccccgtct tttaacagag gccaaagcac gcgcggcatc ggcataattg taaaattgtc
      901 cggcatagta tacataatgg ttcccggcac gtacttcaaa tatggggcta atccctttta
      961 gctgacttat agtggctttt cgcgctaccg taaacaactg tatgcgataa tacaggcccg
     1021 gaggctcgtt ttcccagggg acaatcaccg actcctgctc tatgcgaaat tccaaattta
     1081 ccggtgggac aaccggaagg ggagcttcga acaaatggct gtccaggctg atgagttgtt
     1141 gctttacagg aaataaatcc atttcctgta acccggaagc acttgaggca gggctttttg
     1201 acgcaggcgt cgtaacggga ggcaatacgc catgagatag gaacagatcc tctatggatt
     1261 gtgccgattt tcgcgtatca cgaagggatt gttgcaattc cataagaaca aattcttctt
     1321 ccctaatggt attggcaatc gccctcctgt cttcttcagg tgtaagtgaa ctatagtact
     1381 ttcgcaagct atccagtttt ttttcatggc ccgctacctg gacttttaga ttcctgacgg
     1441 ccgccgtaag ttgggggtag tgggctgtgg cctcccgtgt tttttccagg attgagtcgg
     1501 gcactcccat cccggcttct tttgggccat actccctgtc ccgggttttg tttcccgata
     1561 agggcatcat ggcaatttcc cggagttctt ttaccgaggc tggccgtatt tttaccggag
     1621 cactttcata ttccaccttg tacaagacca gcgaatcttt tggggcggca cggtccgagg
     1681 taaaataaaa ataacgagaa tcatccgaaa gcatgtaaag cagatcgtta tgggtcgagg
     1741 agaagggaat gcccagattt tccgccaggc cccattcctg tgtcttagtg tcccaacggc
     1801 ttacgtaaag gttcatccct cccagcccgg gaagtccgtt tgagctgaaa tacagggttt
     1861 taccatcgga act
//
LOCUS       DAOQXQ010000381         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73834_length_1869_cov_2.547409, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000381 DAOQXQ010000000
VERSION     DAOQXQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73834_length_1869_cov_2.547409"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKH81_06600"
     CDS             complement(<1..359)
                     /locus_tag="PKH81_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ97742.1"
                     /translation="MTKKSNRRVRRVFLFLLALMCVSCIPSRTSPKKEKVRIGFSIAT
                     ATFIIERWNKDIKIFTGAVRDLGAESIVQLSAGGTKEQIDQINFLIASNIDVLVVIAH
                     DTNMLSGVIKKAKDQKIP"
     gene            complement(343..1308)
                     /locus_tag="PKH81_06605"
     CDS             complement(343..1308)
                     /locus_tag="PKH81_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ97743.1"
                     /translation="MVCFIGLDSEQDNVYSRLNLAIDLKWKFLFTMHLNLGMYYFPSP
                     VDPECLISFLEKTIASQIPSGILAITGFGSVRHGTELYLSCTEALGELQHKRVRTDIR
                     LRERLRIVQIRRKLGLSELEELRSIFTEYWEESFSSFPFALAKAKMVALFSLLIDDAT
                     GCYQSSSDTLVPFDPAEELLGVEDIAAWKDWAQAAFNRVYSLARLKRTGQFPVPLVKA
                     LSYIEECYDKQIQLSDVADAAQVSSAYVSKLFTEHLESTFVDYLTEIRVAHAERLIRE
                     NTLTIKEISNAVGYQDPNYFSKIFRKSVGISPSMYAQRSRYDEEK"
     gene            complement(1206..1817)
                     /locus_tag="PKH81_06610"
     CDS             complement(1206..1817)
                     /locus_tag="PKH81_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97744.1"
                     /translation="MYTVLIVDDEEPVIESYSFILEQGVDGFTLAGIARSGPEAVARI
                     NELSPDVVFMDINMPGFDGLEAISRVHECFPNTIFVLSTAYERFDIARKAIPLGVYAY
                     LVKPVTRQLFVETLAGIKGEMEKRRASLSIGQSALLADRFLRETIWKAMDESSWREHR
                     QTLHLDSDKGWCVLSGSIQSKTMYILVLTWLLILNGNFFLPCT"
BASE COUNT          531 a          453 c          424 g          461 t
ORIGIN      
        1 ggtatctttt gatcctttgc ttttttgatt acacccgaga gcatgtttgt gtcgtgggca
       61 ataaccacca aaacatcgat attgcttgca ataagaaagt tgatttgatc aatctgctcc
      121 ttggttccac ctgcggataa ttgaacaatt gattccgctc caagatcccg aacagccccg
      181 gtgaatattt ttatatcctt attccaccgt tcaataataa aggttgcggt agctatagaa
      241 aaaccaatgc gaaccttttc cttttttggc gacgttctgg acgggataca cgatacgcac
      301 ataagagcca acaggaacaa gaagactcga cggacgcgtc tattactttt cttcgtcata
      361 acggctcctt tgcgcgtaca tgcttggcga aatgccaacg ctctttcgga atatcttact
      421 aaagtaattg ggatcctgat agcctaccgc gttagagatt tcttttatcg tcagggtgtt
      481 ttcccgtata agtcgctcgg catgtgctac gcgaatttcc gttagataat ccacaaaggt
      541 agactcaaga tgttcggtaa agagcttgct tacataagct gaggaaacct gggcagcatc
      601 ggccacatcc gaaagttgaa tttgcttgtc atagcactcc tcgatgtacg acagggcctt
      661 aacaagggga accggaaatt gtccggtgcg tttaagccgg gccagggaat acacacgatt
      721 aaatgcagcc tgagcccagt ctttccatgc tgcaatgtct tcaaccccca ggagctcctc
      781 tgcgggatca aagggaacaa gggtgtctga cgaagactga tagcagcctg tggcgtcgtc
      841 gataagcagt gaaaaaagag ctaccatctt tgcctttgca agagcaaagg gaaaactgga
      901 aaaagattct tcccagtatt cagtaaagat acttctcagt tcttccagct cggacaaccc
      961 cagctttcgc ctgatctgca cgatgcgaag ccgttcccta aggcgtatat cagtgcgaac
     1021 ccgcttatgc tgtagctcgc caagggcctc ggtacaggaa agatacaact cggttccatg
     1081 gcgaacagaa ccaaagcctg ttattgccag gataccggat ggaatctggg aagcaatggt
     1141 tttttccaaa aagctgatta agcactccgg gtctaccgga gaaggaaagt agtacatacc
     1201 aagattcagg tgcatggtaa aaagaaattt ccatttaaga tcaatagcca ggttaagacg
     1261 agaatataca ttgtcttgct ctgaatcgag cccgataaaa cacaccatcc cttgtcgcta
     1321 tccagatgaa gggtctgacg atgttcacgc caagaggatt catccatggc cttccagatt
     1381 gtttccctaa gaaagcgatc ggccaagagc gcggattgac ctatggaaag cgaggcgcga
     1441 cgcttttcca tctcgccctt tatccccgca agagtttcta caaacagttg acgggtaacc
     1501 ggttttacca gatatgcata cacgccaagc ggtatagcct tacgagcgat atcgaatcgc
     1561 tcgtacgcag tagacaacac aaaaatagta ttgggaaaac attcatgaac gcgagatatt
     1621 gcctcaaggc cgtcaaagcc gggcatgtta atatccataa aaacaacatc aggagaaagc
     1681 tcgttgattc gggcaacagc ctcgggccct gaacgagcaa ttccggcaag cgtaaaacca
     1741 tccacaccct gctcaaggat aaaggagtag ctttcgataa ccggctcttc gtcgtccaca
     1801 attaagacgg tatacatagg tccctgtcca tatcaatgtt aataagaatc tcggtcccct
     1861 tttcagcag
//
LOCUS       DAOQXQ010000382         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_73883_length_1868_cov_3.108108, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000382 DAOQXQ010000000
VERSION     DAOQXQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73883_length_1868_cov_3.108108"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PKH81_06615"
     CDS             complement(<1..1021)
                     /locus_tag="PKH81_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ97745.1"
                     /translation="MGFFKSLSADIGIDLGTCNTLIYIHGKGIVVNEPSVVAVERGTK
                     RVVAVGAEAKRMLWKTPGNIIAIRPLKDGVIADMDTTEKMIRYFVSKIMPRHGLIKPR
                     MVIGIPSCITDVENRAVKESAFKAGAREVQVIEESLAAAIGAQIPIHEPAGHMICDIG
                     GGTTEVSVISLGGMVVTNAIRLGGDEFDQAIIKHVRSVHNLIIGEQTAERLKVEIGNA
                     SPEKNIEKVEIKGTDAITGLPRRLEIDSVEVREALKDPITQIVEEIKRTLAQTPPELA
                     ADIVERGIVMTGGGALLKGLPKLISKETGVPVILAENPMDCVAIGAGQYYEIFKDMSI
                     DRSIYD"
     gene            complement(1055..>1868)
                     /locus_tag="PKH81_06620"
     CDS             complement(1055..>1868)
                     /locus_tag="PKH81_06620"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97746.1"
                     /translation="WRSRNGALPSMTGLYTDWKNASYTEVHTKTGLILAKRPLDGEAL
                     SLRGFSAYYLYCAQTDPAIAQEYLVDSIVSLRNAWYRVSSIDKPVVAYILGKGYYQRG
                     FYYADLAMKYLAYARDSGLQRDDLSEYIGLTADLLGDNALAIASFTEALASSPSDLLL
                     FTLSRSYLKAEDIDKAKQYFLETIRVTSDDLLANRCRFELGMILLKEERFDEAESEFT
                     TIMEKEPNSADAQYGLGVLYEARGELVKARAAWRKALRLNPVHSGARQKMNI"
BASE COUNT          504 a          456 c          392 g          516 t
ORIGIN      
        1 tgtcgtatat actgcggtca atggacatat ctttaaagat ttcataatac tgaccggcgc
       61 ctattgcgac gcaatccatc ggattttctg caagaataac aggaactcct gtttcttttg
      121 aaatcagttt gggaagaccc tttaacagtg cgccaccacc ggtcataaca ataccgcgtt
      181 caacaatatc agcggccagc tcaggaggtg tttgcgcaag agtacgctta atttcttcaa
      241 cgatctgggt aataggatcc ttaagggctt cgcgaacttc aacagagtca atttcaagcc
      301 gtctcggtag tccggtaatt gcatcagtgc ccttaatttc gactttttca atattttttt
      361 ctggagaggc atttccaatt tctaccttaa gccgttcagc ggtttgctca ccgataataa
      421 ggttatgaac tgaacgaacg tgcttaatta tagcctgatc aaattcatct ccacctaaac
      481 ggatagcatt agtgactacc atacctccga gtgaaattac cgatacctcg gttgtgccac
      541 caccaatatc acaaatcata tgtccagcgg gctcatgaat ggggatttga gctccaattg
      601 ccgctgcaag agactcttcg attacctgta cctctcgtgc accggcttta aaggcacttt
      661 cttttaccgc acggttctca acatccgtaa tgcagctggg gattccaata accattcgcg
      721 gcttaatcaa tccatggcgg ggcattatct tggatacaaa ataacgaatc attttttctg
      781 ttgtgtccat atcggcgatt accccatctt tgaggggccg tatggcaatg atgtttccag
      841 gggttttcca tagcattcgc ttggcttcgg cgcctacggc aacaacccgc ttggttcccc
      901 gttcaactgc aacaactgaa ggctcgttta ccacaatgcc tttaccatgt atgtaaatca
      961 gggtattgca ggtaccaaga tcaataccaa tatccgccga aagactctta aaaaaaccca
     1021 taaaagacgt tcctccacac tatagtaccg tttatcaaat attcattttt tgccgtgctc
     1081 cggaatgaac cggattaagg cgcaaagctt tacgccatgc tgcccgggct tttaccagct
     1141 ctccccgggc ttcatatagt acaccaagtc cgtattgtgc gtcagcagaa ttcggctctt
     1201 tttccataat tgtcgtgaat tcgctttcag cttcgtcaaa tcgctcttcc ttaagaagaa
     1261 tcatccctaa ttcaaaacgg catcgattag caagcaggtc atcactggta acccgtatgg
     1321 tctcaagaaa atattgtttg gccttgtcaa tatcctctgc ctttagatag ctacgtgaaa
     1381 gcgtaaataa aagaagatca gagggacttg aggcaagagc ttcggtaaac gacgcaattg
     1441 ctaatgcgtt atctcccaat aaatcggctg ttaaaccgat gtattcacta aggtcatctc
     1501 gttgtaggcc agaatcacgg gcataagcaa ggtatttcat tgctaaatcc gcataataaa
     1561 aaccgcgctg atagtagccc ttacccagaa tgtaggcgac aactggtttg tcaattgatg
     1621 agacgcgata ccaggcattc ctgagggaca cgatagaatc tacaaggtat tcctgagcta
     1681 tcgccggatc agtctgtgcg caatataagt aatacgccga gaagcctctt aaggataaag
     1741 cttctccatc aagcggacgt tttgcaagaa tgaggcctgt ttttgtatga acctccgtgt
     1801 acgaggcatt cttccagtca gtatacaagc cggtcatcga agggagggcg ccgtttcggc
     1861 tacgccag
//
LOCUS       DAOQXQ010000383         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_74124_length_1865_cov_2.248619, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000383 DAOQXQ010000000
VERSION     DAOQXQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74124_length_1865_cov_2.248619"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKH81_06625"
     CDS             complement(<1..887)
                     /locus_tag="PKH81_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNQ97747.1"
                     /translation="MSREGFTWIDAHSKIFDYLSEHRNDQPGLIQLLRDLEITALNDR
                     DENDQTIELTEIDPFTFFCYLYKHGPDRNLTNLQRLSDRLGINPRPTDIQGLPSAYAQ
                     KVWLFPYKKTRTHNEVPRLWAFFDAVRDNKVNETLFEDILTIKNVGLVKLTEALFMLQ
                     PEKYLPINGPVRPWLLTQYGIDAKFKTWTEYLNILTTIRTKTELSFPEISYATWITRQ
                     ESPVGYWIFQGNPASFDLVTALQKNLLTTWSVTAHKNEIKAGDKVIIWATGKEPGCYA
                     LAEVTDKPGSAQPAPDDHL"
     gene            complement(932..>1865)
                     /locus_tag="PKH81_06630"
     CDS             complement(932..>1865)
                     /locus_tag="PKH81_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNQ97748.1"
                     /translation="LEKSYEEYMKGFTDIASGEARRGYLEVVKELQSRFPNPDEIVTE
                     KDKKAFTKLFGEYLRVESILQNYDEFAGLQALQHLDTTNPEAVEEFKATYYLNDEDIA
                     AMREIKVPPVRMIQDYRSTYNDIRDWLRRESNAKQKDESSIDWSDVVFEVDLLKSQEI
                     NLDYILELIFENNKKRKDKAALIEDVRRLIRSSIGNRAKESLLVDFINHTNLDAIQDK
                     AHVIDAFFAYAQAEQQREVNELIAEENLNEEAARRYITISLKREYASENGTELNAILP
                     KMSPLNPQYLKMKHTVFQKIADLVEKFKGVGGKI"
BASE COUNT          426 a          418 c          427 g          594 t
ORIGIN      
        1 caaagatgat catcgggggc gggttgggcc gagccgggct tatcggtaac ttccgcaagg
       61 gcatagcaac cgggttcttt tcccgtagcc cagattatta ctttgtcccc cgctttaatt
      121 tcgttcttat gggcagtcac cgaccatgtc gttaacagat ttttctgtaa agccgtaaca
      181 aggtcaaagg aagcagggtt tccctggaat atccaatacc ccacaggcga ttcttgccgg
      241 gttatccaag ttgcatacga gatttctgga aatgacagtt ctgttttagt gcgaatggtt
      301 gtcaggatat tgaggtactc agtccatgtt ttaaactttg catctattcc atattgagta
      361 agaagccaag gacgaacagg tccattgatg ggcaggtact tctcgggttg cagcatgaat
      421 agggcttcgg ttaatttcac cagtccaacg tttttgatgg taagaatatc ttcaaaaaga
      481 gtttcgttta ctttgttatc tcgaactgca tcaaaaaatg cccagagccg tggtacttca
      541 ttatgtgtcc gtgttttctt gtatgggaac aaccagactt tttgggcata tgcagagggt
      601 aatccctgta tgtcagtagg tcgcgggttg attccgagtc gatcggaaag gcgctgaagg
      661 ttggttaagt ttcgatccgg tccatgtttg tagaggtaac aaaagaaggt gaacgggtcg
      721 atttcggtga gttcaatagt ctggtcattt tcgtccctgt cgttgagagc ggtgatttcc
      781 agatcacgga gtagctggat taatcccggc tggtcattcc gatgttcgct caggtagtca
      841 aatatcttgc tgtgcgcgtc gatccaggtg aatccttctc tgctcatgtt gttttccttt
      901 ttgtgcttcg tggttttcta atattcatcg cttatatctt tccgcctacg cccttaaact
      961 tctcaaccaa atccgcaatt ttctggaaca ccgtgtgttt catctttaga tattgtgggt
     1021 taagcggact catcttaggc agaattgcat tgagctcggt tccgttttcg cttgcgtact
     1081 cccgtttcaa tgaaatggta atgtatcgcc ttgcagcttc ctcattcaga ttttcttcgg
     1141 caattaactc gttgacttcg cgctgttgct ctgcttgggc atacgcaaaa aaggcatcga
     1201 tgacatgcgc tttgtcttga atggcatcaa gattcgtgtg attgataaaa tcgacgagca
     1261 aactttcctt tgctcggtta ccgatactgg acctgataag ccttcgaacg tcctctatca
     1321 aggcggcttt gtctttgcgt tttttgttat tctcgaagat taactcaaga atgtaatcaa
     1381 gattgatttc ctgggatttc aatagatcaa cttcgaaaac gacatcggac caatcaatag
     1441 aggattcgtc tttctgcttc gcgttacttt ctctccggag ccaatcccga atatcattat
     1501 aggtcgaccg gtaatcctga atcattcgaa cggggggaac tttgatttcc ctcattgcag
     1561 caatatcttc gtcgttcaag tagtacgtgg ccttgaactc ctcaactgcc tcagggttcg
     1621 tcgtatcaag gtgctgcaag gcctgcaatc cggcgaactc atcgtaattc tggagtatac
     1681 tttccacgcg cagatattca ccgaatagct tggtaaaagc ctttttgtct ttttccgtta
     1741 cgatttcatc gggatttggg aatctggatt gaagttcttt caccacctcc aggtatccac
     1801 gtcgggcttc gccgcttgca atatcggtaa agcctttcat gtactcttca taactctttt
     1861 ccaga
//
LOCUS       DAOQXQ010000384         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_74690_length_1856_cov_2.760133, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000384 DAOQXQ010000000
VERSION     DAOQXQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74690_length_1856_cov_2.760133"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..808)
                     /locus_tag="PKH81_06635"
     CDS             complement(71..808)
                     /locus_tag="PKH81_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ97749.1"
                     /translation="MSGHSKWATIKHAKGAADAKRGQMFTKFIKEISIAARMGGGDAN
                     ANPRLRTAILKARAANMPKDNIDRAIKKGTGELGATTFEELLYEGYGPGGVAILVEVL
                     TDNKNRAAANVRNLFSKNGGNLGATGSVAYMFNRKGVIEYDASVCDEDMIMEVGLEAG
                     ADDVANDDGVITVTTDPLNFEAVLEALQAKGLESLSAAISMVPDTYSELDVDTTRKVL
                     KLVDKLEEDDDVQNVYSNLDIPEGFEE"
     gene            complement(957..1181)
                     /locus_tag="PKH81_06640"
     CDS             complement(957..1181)
                     /locus_tag="PKH81_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97750.1"
                     /translation="MKSLYCDICKNEIENPISERTCFHIKEYDICEACHDALFARLRP
                     TVRSHFPYSSEWYEQQFTALVERSMATSRI"
     gene            1294..>1856
                     /locus_tag="PKH81_06645"
     CDS             1294..>1856
                     /locus_tag="PKH81_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNQ97751.1"
                     /translation="MSHVQIIAEIGTAHGGSIERCRQLIQSAKGAGADTAKFQIVYAL
                     EILHPKSGIVPLPGGDIPLYERFTALELPLDFYRQCKAICDGEHIGFLCTPFGMESAQ
                     ELTTLSPQAVKIASPELNYLDLIREVASWGVPLILSSGVSLLSDIEAALETAAQAGCP
                     KHLITLLHCITSYPAPEEEYNLRLLATL"
BASE COUNT          468 a          495 c          447 g          446 t
ORIGIN      
        1 ccagccggta gaagccaggc ccggatcaat gccaagaacc agacggtcac tgttatgcgt
       61 aatcagagga ttactcttca aaaccctcgg ggatgtctag attggaatac acgttctgaa
      121 cgtcgtcgtc ttcttcgagc ttgtctacca gcttaagtac cttgcgagta gtatccacgt
      181 caagttcgct gtaggtgtcc ggaaccatgg agatagcagc ggaaagggat tcaaggccct
      241 tagcctgcaa ggcttcaaga acggcttcaa agtttaaagg atcggtggtt acggtaataa
      301 ccccgtcatc gtttgcaaca tcgtcagctc cagcttcaag gccaacttcc ataatcatgt
      361 cttcgtcgca gacagacgca tcgtattcga taacgccctt gcggttaaac atataggcaa
      421 cagagccggt agcaccaagg tttccgccgt ttttggaaaa gaggttacga acgttggcag
      481 cagcacggtt tttattgtcg gtaagtactt ctaccagaat ggcaacgccg ccggggccgt
      541 atccttcgta taatagctct tcaaaggtgg tagctcccag ttcgccggtt cccttcttga
      601 tagcccgatc gatattatcc ttgggcatgt tagccgcgcg cgccttaaga atggcggtgc
      661 gaagacgggg gttagcgtta gcatcaccgc cgcccatacg agccgcgata gaaatttcct
      721 tgatgaactt agtgaacatc tggccgcgtt ttgcgtcggc ggctcctttt gcgtgtttaa
      781 ttgtcgccca tttactatgt cctgacatga cgtctccttt atggttaccg ggtataaggt
      841 ttacaaataa agatagtacc atagcgcaaa aaaccatgtc aaggcgcgaa aaaacgcagt
      901 gttttgcaaa aaaaaaccgt ccccggttgg ggacggcata gcagacaaca ggagtcttaa
      961 atgcggcttg ttgccattga gcgctctacc aaagcggtga actgctgctc ataccattcg
     1021 ctggaatagg gaaaatgaga acgtacggtc ggcctcagac gggcaaacag ggcatcgtgg
     1081 caggcttcgc agatgtcgta ttcctttatg tggaaacacg ttctttcgga aataggattt
     1141 tcaatttcgt tcttgcagat atcgcaatac agggatttca ttcaaacctc cataacaaat
     1201 acatctttgt agttatcata gcgcatcggc tctagtttgt cgagcaagtt ttttaccggt
     1261 tgacagcccc caaagagcgc gtacaatcac cgtatgagcc atgtacagat aatagccgag
     1321 atcggaaccg cccatggagg atccatcgaa cgctgccgac agttgatcca gagcgccaaa
     1381 ggagccgggg cagacaccgc caaattccag atagtgtatg ctctcgagat tctccacccc
     1441 aaatcgggca ttgtccccct tcccggcggg gatataccct tatatgagcg ctttaccgcc
     1501 cttgaattac cgctcgattt ttatcgtcaa tgcaaggcta tctgcgatgg agaacacata
     1561 ggctttcttt gtaccccatt tggcatggaa agcgcgcagg aacttaccac cctaagcccg
     1621 caggccgtaa aaatagcctc accggaactc aattatctgg accttatacg cgaagtagcc
     1681 tcctggggcg ttcccctcat cctttcaagc ggagtgtcgc ttctttcgga catagaagcc
     1741 gccctggaaa cggccgcaca ggcgggctgt ccaaaacacc taattaccct cctccactgc
     1801 atcaccagct acccggcacc ggaagaagaa tataacctcc gcctcctggc caccct
//
LOCUS       DAOQXQ010000385         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_74814_length_1854_cov_3.429683, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000385 DAOQXQ010000000
VERSION     DAOQXQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74814_length_1854_cov_3.429683"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..>1854
                     /locus_tag="PKH81_06650"
     CDS             234..>1854
                     /locus_tag="PKH81_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97752.1"
                     /translation="MRADRYSNALAVYLKLSELDMDNVEVLNNLGIIYRRLEQYDKSI
                     EVLTRARKSGSDFETVLYNLGNTYKQMGRYEEAAACFLEVIEQKPDDVLAYNHLGSIH
                     ALSGRHDLALQAYRRGLQIDPNHPFIHFNMAHSYELLSRPDEAVSEYNAALKTKPGWL
                     EALRDLAVLYSRQKDEAKSTATWRRILAVDSSDVRALTELGNIAARSQRNEEALSYYR
                     KAINSDAGYIHAVIGLVQLLEKQGKMEDALQELLSVKEKNPQAIDLLINLARLYIKLQ
                     QYPEAKVVFRDLERIAPDNSASLRLQGRMYAQLGDNEQAELCFARILALNPAEIDFRI
                     DLADQLVQSGKLPEAEEQLLTYLNERPRDYPVWISLGQLYENMGRNEQALETYKDVIS
                     REPDNIDSRSALSQLYQKLGMNTEAVHLADEIVNLQGSRGTTEDLSALTQSLELYEQA
                     VHGYGASNPGALSRNLDLLRSRSITDDAFSAESEESVPEAQSPSFVPVDEETDPMSLI
                     EDDELIEDSELPFDELVSEDEELPDDDLETLD"
BASE COUNT          460 a          436 c          465 g          493 t
ORIGIN      
        1 tttccatgga agtttctgct cggcctcgtg ccggtcacgg atcatctgcg cctcggagca
       61 attactaatg tgtaagtgga gaatgcatgg ctgaccaatt atctgtactt attgaccggg
      121 ccaattcagc cttggtggcg cgggactttt cctaagccga gaagttatta actaatgcac
      181 tcaagcaaca tcctgacgcg accgaggccc tgtcgctttt ggggacggta tatttgcggg
      241 ccgatcgata ttcaaacgcg cttgctgtgt atcttaaact ttccgagctt gatatggata
      301 atgtcgaggt tctaaataac ctcgggatta tttatcgtcg gcttgagcaa tatgataaat
      361 caatcgaagt gctcactcgc gcgcgaaaaa gcggcagtga ttttgaaacg gtgttatata
      421 acctgggaaa tacctataag cagatggggc gatacgaaga agccgccgcc tgtttccttg
      481 aggttatcga gcaaaaacct gatgacgtgt tagcctataa tcacttggga agtattcatg
      541 ccctaagcgg tcgccatgat cttgccttac aagcttatcg ccgtggccta caaattgatc
      601 ccaatcatcc ttttattcat tttaatatgg ctcatagcta tgaactgctt tcccgtccgg
      661 atgaggcggt ctctgaatat aatgccgcct taaaaacaaa accgggttgg ttggaagccc
      721 ttcgtgatct ggctgttctg tatagtcggc aaaaagatga agcaaaaagc actgccacct
      781 ggcggcgtat tcttgcggtg gactcttccg atgtgcgcgc tcttaccgag ttgggaaaca
      841 ttgccgcccg ttcgcagcga aacgaagaag cgctttcgta ttatcgaaag gcaattaata
      901 gcgacgcagg ttatattcat gcggtcatcg gtcttgttca gttgctggaa aagcaaggaa
      961 aaatggaaga tgccttgcag gaacttttat ccgttaagga aaaaaatccc caggccattg
     1021 atttactgat taaccttgca cgcctgtata tcaagcttca gcaatacccc gaggctaagg
     1081 ttgtgtttag ggatttggaa cgcattgctc cggacaatag cgcctccctg cgattacagg
     1141 gacgtatgta tgcccagctt ggggataatg aacaagcgga attgtgcttt gctcgcattc
     1201 ttgcccttaa ccccgcagag attgattttc gcatcgattt ggctgaccag cttgttcagt
     1261 ccggtaaatt gcccgaagcg gaagagcagc ttcttaccta tcttaacgag cgccctcgtg
     1321 actatccagt ttggatatcg cttggacaat tatacgagaa tatgggcagg aatgaacaag
     1381 ccctggaaac ctataaggac gtaatctcgc gggaacccga caatatcgat tcccgctctg
     1441 ccctgtctca actctatcag aagcttggta tgaacaccga ggcggtgcat cttgctgacg
     1501 agattgtaaa tcttcaggga agccgtggta ctaccgaaga tctttcggcc ttgacccagt
     1561 cgcttgaatt gtatgaacag gcggttcatg gatacggggc gagtaatcct ggtgccttga
     1621 gtcgtaacct ggatttattg cgctctcgct ctattaccga tgatgcgttt tctgcggaat
     1681 ccgaggaatc ggttcctgag gcgcaaagcc cttcatttgt tcccgttgat gaagaaaccg
     1741 atccgatgtc ccttatcgaa gacgatgaac tgatcgagga ctccgagctt ccctttgatg
     1801 aactggtttc cgaggacgaa gagcttccgg acgatgacct ggaaacgctg gacg
//
LOCUS       DAOQXQ010000386         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_75548_length_1843_cov_3.190157, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000386 DAOQXQ010000000
VERSION     DAOQXQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75548_length_1843_cov_3.190157"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="PKH81_06655"
     CDS             <1..1132
                     /locus_tag="PKH81_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNQ97753.1"
                     /translation="LRSYVSSGMSPYDHDDLTDPDDVRILSRTSIKQYLLASENSFRL
                     YTKMMFVHILVSQLRGDKARKKNAREDVWRAQRNEIFRLSTLQSGSDARVLRSLAYKH
                     LLIAEKMTRVRGVFSPSVTASDFDMDTLKEYLCQLDKINVYIHPLGGKVFELDVFSAY
                     RNYCDLSLPSSSGLFIDHFINSEELDIFIDKGVLPDAPVFSSQVYQDLSIDKSRIEVQ
                     LRANGLFGSFQQPLQLKKQYTVRNEGIQVQYILKNESPLALSGFFMTELDLSFSRNRH
                     KQTLMTVYAQETRQDGPVVKSAYKDIEWMQISDNGSGVKFTFEANETPSVAILPVLCP
                     DDSNQGDDASVTSEGARLFFYWKIELAANYEMEKTIFFKIDS"
     gene            1190..>1843
                     /locus_tag="PKH81_06660"
     CDS             1190..>1843
                     /locus_tag="PKH81_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ97754.1"
                     /translation="MKRKLVTSALPYVNNIPHLGNLIQVLSADVYSRFCRLRGYSTLY
                     VCGTDEYGTATETRAQEEGKTPRELCDYYNAIHRDIYSWFAIAFDYFGRTSTPQQTEI
                     TQGIFKDCDANGFIQEHTIEQLFCVSCNRFLADRYVRGICPSCGFEDARGDQCESCGK
                     LLDPTELKKPRCATCAKEPEIRTTSHLYLDLPAILPEYEKWMKSASVNGQWAKNAIQM
                     "
BASE COUNT          549 a          397 c          383 g          514 t
ORIGIN      
        1 gctcagaagt tatgtctctt ccggaatgtc accttatgat catgatgatt taactgatcc
       61 tgatgatgtc cgcattcttt ccagaacttc aataaagcaa tatttactgg ccagcgagaa
      121 ctcatttcgt ctgtatacaa agatgatgtt tgtccatatc ctcgtcagtc agcttcgagg
      181 agataaagcc cgaaaaaaaa atgcaagaga agatgtatgg agagcacaaa gaaacgaaat
      241 ttttcgctta agcaccttac aatcaggatc agacgcacgg gttcttcgat cgcttgctta
      301 caaacatttg cttatcgcag aaaaaatgac gagggtaaga ggagtttttt ctccttcagt
      361 tacagcctcc gattttgata tggatacgct caaagaatac ttatgtcagc ttgataaaat
      421 taatgtgtat atccatcccc ttggaggaaa ggtttttgag ctggatgtat tcagtgcata
      481 ccggaattac tgtgatcttt cccttccgtc atcttctggt ctatttatag accactttat
      541 aaatagcgaa gaactagata tctttataga taaaggcgtt cttccggacg caccagtctt
      601 ttcatcccag gtctaccaag acctgtcaat cgataagagc aggattgagg ttcaattacg
      661 agcaaatggt ttatttggct cttttcaaca acctttacaa ttaaaaaaac aatatactgt
      721 gcgtaacgag ggaatccagg ttcagtacat cctgaaaaat gaaagtcctc tggcgctctc
      781 tggcttcttt atgaccgagc ttgacctgtc tttttcccgc aatcggcata aacagacatt
      841 aatgactgtg tatgcccaag aaacccggca agatggtcca gttgttaaaa gtgcctataa
      901 agatattgaa tggatgcaaa tatccgataa tggaagcgga gtcaagttta catttgaggc
      961 gaacgaaacc ccatcggtag cgatcctccc cgtactgtgt cccgatgatt caaaccaggg
     1021 agatgatgct tcggtaacga gcgagggcgc cagactcttc ttttactgga aaattgaatt
     1081 agccgcaaac tatgaaatgg aaaaaacaat cttctttaaa attgattcct gaccttgcta
     1141 cccgttaatc ccgttgactt gattgaccgg aaaaattatc atagtaacca tgaaacgaaa
     1201 actcgtcact tcagcacttc cttatgttaa taatattccc catctgggga accttataca
     1261 ggttctttcc gcagatgttt attcgcgttt ttgtcgttta cggggctatt caacccttta
     1321 tgtatgcggc actgatgaat atggaacagc gactgaaacc cgagcccaag aggaggggaa
     1381 aacgccccgg gaactatgcg attattacaa cgcaattcac cgtgatattt actcctggtt
     1441 tgccattgcc tttgattact ttggcagaac gtcaacaccc cagcaaacag aaataacgca
     1501 ggggatattc aaagattgcg atgcaaatgg ctttatccaa gagcatacca tagagcagtt
     1561 gttttgtgtt tcttgtaatc gcttcctcgc agaccggtat gtccgtggaa tttgtccttc
     1621 atgcggtttt gaagatgctc gaggcgatca atgcgaaagt tgcggaaaac tgctagatcc
     1681 taccgagctt aagaaaccac gttgtgcgac ctgtgcaaaa gaaccagaaa ttcgaactac
     1741 aagccattta tatcttgatc ttccagccat cttgcccgaa tacgaaaaat ggatgaaaag
     1801 tgcaagtgtg aacggtcaat gggccaagaa cgcaatacaa atg
//
LOCUS       DAOQXQ010000387         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_75883_length_1838_cov_2.973640, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000387 DAOQXQ010000000
VERSION     DAOQXQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75883_length_1838_cov_2.973640"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..996
                     /locus_tag="PKH81_06665"
                     /pseudo
     CDS             118..996
                     /locus_tag="PKH81_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759434.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
     gene            996..>1838
                     /locus_tag="PKH81_06670"
     CDS             996..>1838
                     /locus_tag="PKH81_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNQ97755.1"
                     /translation="MENEQMSHKSKEALFDEARAIWAEIQDKTLSPKDRMAIPPQEMP
                     AGEPSERARQMSEVALGYSEEQARVEAARCLGCKNRPCVAACPVGVPIPEFIACIQDG
                     DFAKAVATIKMTNLLPAICGRVCPQERQCQSACTVGKSLKTVDKAVAIGRLERFVADW
                     ERENGLIKAPEVSAPTGKKVAVVGSGPAGLTVAADVRRAGHEVTIFEAFHKTGGVMVY
                     GIPEFRLPKAIVAKEVETLKEMGVHFRTNFLVGRTETLYNLLDKEGFDAAFIGTGAGL
                     PKFMD"
BASE COUNT          484 a          398 c          516 g          440 t
ORIGIN      
        1 gaaaacacac ggagcattgc acctgcttgt tttctatgca atgcattgta taaatatgca
       61 taaaaatggt agtatgacac aattatctaa cctgtctaga agtcaaaggg gacacagatg
      121 cataccatac tagagaaacg gcaattctcc cagtaggttt tttacctccg tgttacggct
      181 ccggaaattg cccgtaacag aaaagccggc cagtttgtgc tggttcagat tgataccgac
      241 tttggcgaaa gaatccccct caccatcgcg gatgccaacg cggcagaggg ttggattgcc
      301 ctggtgtttc agactgttgg cgctacaacc cataaactcg cgcttaagaa tcctggagac
      361 aaaatagaag caattcttgg cccgctagga agaccgaccc atatcgccaa ggtaggaacc
      421 gtggtgtgcg tgggtggtgg cattggggtt gctcccttgt acccgatagt aaaagcatat
      481 aaggaagcgg gcaataaggt tatcgttatt attggtgcgc ggaataaaga gctggttatt
      541 tttgaagaag aaatgcgcgc tgccagtgat gaattgattg tggttaccga tgatggatcg
      601 tatggacgca aggggctggt aaccgaaccg cttaaggaat tgtgcgagag cgcgactccc
      661 ccgagcgagg cagttgccat cgggcctccc attatgatga agttctgtgc cgctactacg
      721 aaaccctacg gtgtgcatac tgttgtatcc cttaacacca ttatgatcga tggaacagga
      781 atgtgcggag gatgccgcgt aactgttggc ggaaaaacca agttcgtctg tgtggatgga
      841 cctgagtttg acggacatca agttgatttt gacaacatga tgatgcgtat gaaggccttt
      901 aagggacgag aagaagaaga tcatcataaa tgtcacatgg aaatggaagc cgaccgcgtg
      961 ggcgatcccg attctgataa gccctccagg gcgtaatgga gaatgaacag atgagtcaca
     1021 agagcaaaga agcattgttt gatgaagctc gcgccatatg ggcggagata caagataaaa
     1081 ccctttcccc caaggatcgc atggcgattc ccccgcagga aatgccggca ggggagcctt
     1141 ctgaacgggc tcgacaaatg agcgaggtcg cattggggta ttccgaagag caggctcggg
     1201 tagaggcagc tcgttgtctt ggctgcaaga atcggccctg tgtagcagcc tgtccggtag
     1261 gggttccgat tcctgagttt attgcctgca tacaagatgg tgactttgct aaggcagttg
     1321 caaccattaa aatgaccaac ctgctcccgg caatttgcgg acgtgtgtgc cctcaggaga
     1381 gacaatgtca aagcgcatgt actgtaggta aatcccttaa gaccgtggac aaggcagttg
     1441 caattggccg cctggaacgc tttgtggcgg actgggagcg ggagaatgga ttgattaagg
     1501 ctccagaagt atctgctcca accgggaaaa aagttgcggt ggtaggttct ggtcctgccg
     1561 ggttaaccgt tgccgctgac gttcgtcgag ccggacatga agttaccata tttgaagctt
     1621 ttcataagac cggcggggtt atggtatatg gaattccaga attccgtctt cctaaggcga
     1681 tcgttgccaa ggaagtggaa accctaaaag aaatgggcgt gcattttcgt acgaactttc
     1741 tggttggacg caccgaaacc ttatacaatc ttcttgataa agaaggtttt gatgctgcgt
     1801 ttatcggaac tggtgcaggc ttgcccaagt ttatggat
//
LOCUS       DAOQXQ010000388         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_76411_length_1830_cov_2.744225, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000388 DAOQXQ010000000
VERSION     DAOQXQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76411_length_1830_cov_2.744225"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKH81_06675"
     CDS             <1..199
                     /locus_tag="PKH81_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97756.1"
                     /translation="QRHAAEKQFTAHRSPDSIKVALAKTAAEEDPVVRLEKEKKRLEK
                     TIAMLKSRLEAVEKSLSGMEG"
     gene            296..520
                     /locus_tag="PKH81_06680"
     CDS             296..520
                     /locus_tag="PKH81_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97757.1"
                     /translation="MSAPRVSPPAKNKTDETAPRKSAKAASSGELSALLPLLGLDGES
                     SLLQKIRGNGVDSDRDERLESLLEKALSRQ"
     gene            557..940
                     /locus_tag="PKH81_06685"
     CDS             557..940
                     /locus_tag="PKH81_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97758.1"
                     /translation="MEPVSLVRCLVEGNDLLPSFGSSLTASLASDGTFLVTGDRFLVL
                     SGMRRRETVYLLCRKTSGGYYLSVDVSQEQENPASFFWRLSRKSPVAGERIGELVLFR
                     SASPDLAFDVLFRITSSSVKDTSVR"
     gene            960..>1830
                     /locus_tag="PKH81_06690"
     CDS             960..>1830
                     /locus_tag="PKH81_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97759.1"
                     /translation="MNIHRPSSRSLIAALLIFTAAVLTAESAATLVNNMGKAIGASRF
                     NPSFPYALTWKGAADFASLEPSSYAHVPTLDETRIQTRDNPRLQSVLLNNDIFALYGK
                     PDARNMGIVGQYSLTEIESVMNEFVESYDKANGERGVIPAFYLIYGTCWPEGEIGILR
                     DALIVSYIEFALERGWYVFLDHQIGKHSVETSVKQLLPWLKYPNVHLALDPEWRTTRP
                     MQEIGSVTGAELNIAQNMIQEYIISHDLPGRRMLVVHQFKPRMILNRAEVQSDFERVK
                     LIHCADGFGSPALK"
BASE COUNT          428 a          453 c          454 g          495 t
ORIGIN      
        1 gcagcggcat gcagcagaaa aacaatttac tgctcaccga agccccgact ccattaaggt
       61 cgctctggca aaaaccgcag ctgaagagga tccggtggta cggcttgaaa aagaaaagaa
      121 gcggctggaa aaaactatcg ccatgcttaa aagccgcctt gaagcggtag aaaaaagcct
      181 ttcggggatg gagggatagg gagtgctcgt aatcgttgta ctcttattgg tatctttccc
      241 cctgtattcc caaccaacac ccccttcgcc tcctgtccca ccatccgtgc caacagtgag
      301 tgctccacga gtgtcccccc ctgcaaaaaa taaaacggat gagactgctc cccggaaatc
      361 ggccaaggct gcttcctccg gagaactttc tgccctgctc ccgcttttag ggcttgatgg
      421 agagagctcg ctattgcaaa agatacgtgg taatggagtc gactccgacc gtgatgagcg
      481 gcttgaatct ttgttggaaa aggctctctc ccgccaataa tcgacgcctt cgattgagcc
      541 ttctttggag ccttctttgg agcctgtttc attggtgcgc tgtctggttg aggggaatga
      601 cctcttaccc tcttttggct cctcccttac ggcctctctt gcctcggatg gcactttttt
      661 agtaactgga gacaggtttc tcgttctctc tggaatgcgt cgcagggaga cagtgtacct
      721 gttatgcaga aagacttccg gtggctatta tctatcggtt gatgtgagtc aggagcaaga
      781 aaatccagcg tctttttttt ggcgcttgag ccgaaagtct cctgtcgcag gagagcgtat
      841 tggagagttg gtcctgttcc gttcggcctc tcccgatctt gcttttgatg tgcttttccg
      901 cattaccagc tcttcagtca aggatacttc tgtccgatag ttaaaagaca ggagccacaa
      961 tgaacatcca tcgaccttcg agccgatcgc tgatagcggc attgctcatt tttacggccg
     1021 cggtacttac ggcggaaagc gctgcaactt tggtaaacaa catgggtaag gctattggcg
     1081 cctcccggtt taatccctcc ttcccttatg cgcttacctg gaagggtgca gccgattttg
     1141 ccagtcttga gccttcttcg tatgctcatg tgcctaccct ggatgaaacg cgtatacaaa
     1201 cccgggacaa tccccgttta cagtccgttc ttttaaataa cgatatcttt gctttatacg
     1261 gtaaacccga cgcccgtaat atgggtatag tcgggcagta ttcccttacc gaaattgagt
     1321 cggtgatgaa tgaatttgtg gaatcctacg acaaggcaaa tggggagaga ggggttattc
     1381 cggcatttta tttaatctat ggaacctgtt ggcccgaggg cgaaattggc atcttacgcg
     1441 atgccttgat tgtttcgtat attgagtttg cccttgaacg gggctggtat gtgtttctgg
     1501 atcatcagat agggaaacat tcagtggaaa cttcggtcaa gcaactgctt ccctggctta
     1561 agtatcccaa cgtacatctt gccttggacc ctgaatggag aacgactcgc cccatgcagg
     1621 agattggttc ggttacaggc gcagaattaa atatcgctca gaacatgatt caggaatata
     1681 tcatctctca tgatcttccc ggaagaagaa tgcttgtagt tcatcaattc aaaccgcgta
     1741 tgatcctaaa tcgtgcagag gtacaaagtg attttgagcg ggttaagctc attcattgcg
     1801 cagacggatt tggctcaccc gcgctcaaga
//
LOCUS       DAOQXQ010000389         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_76558_length_1828_cov_2.636774, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000389 DAOQXQ010000000
VERSION     DAOQXQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76558_length_1828_cov_2.636774"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKH81_06695"
     CDS             <1..552
                     /locus_tag="PKH81_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97760.1"
                     /translation="VGLAHALIHDPEILILDEPTSGLDPNQIIEVRKLIKEIGRTRTV
                     IISTHILSEVESLCDRIIIINHGRIVADSPTAEIRTRVGNRSTVRVQMAGSKAASHLS
                     ALQRLSGVASVSSFEGESVRDAQGKEKRLCGYVLSVTDDKDPRPEVFALARDNNWIIY
                     EMALEKNSLEDVFRTLTIGGDDE"
     gene            549..1271
                     /locus_tag="PKH81_06700"
     CDS             549..1271
                     /locus_tag="PKH81_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97761.1"
                     /translation="MKSPRPASLVIARRELAAYFSGPIAYIVSGLFLAFTGFLFFSTF
                     FLVNRAELRGFFALLPIMFAFFVPALTMRLFSEEWRSGSIETLLTLPVSSLDVSLGKF
                     AAAFCFIVCMLLPTLSYVITATVLGAPDAGPIIGGYLGALFLAASFSAIGLFASSLTA
                     NQIVAFFIAFSISIALALVDIFLVILPAPVVGFLQFISAGAHFQSISRGILDSRDLLY
                     FVSLTAAFLALTVKTLDLRRAG"
     gene            1268..>1828
                     /locus_tag="PKH81_06705"
     CDS             1268..>1828
                     /locus_tag="PKH81_06705"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNQ97762.1"
                     /translation="MKKKLTSLWAWICGPKSDVALFLVALVLLNLVAGRAFVRIDLTA
                     QGSYSLSRSSVEVIKSLDEPLAIKVFFTEKLPAPYNSVERYLRDLLVEYSGVGSKKFS
                     FEFFDMDKEENQKIASSYGLSLVQVEEYKDNEVGIKNGWMGLAIVYRDRIETLDRLQT
                     PEGLEYRITTTISRMIATTSSLAGLSD"
BASE COUNT          386 a          467 c          456 g          519 t
ORIGIN      
        1 gttggccttg cccatgccct gattcacgat ccggaaatac tgatattgga tgagccgacg
       61 agcgggctcg atcccaatca gattatagag gtgcgaaaac tgattaaaga aatcgggcgc
      121 acccgcacgg ttataatttc cacccatatt cttagcgagg tggaaagcct gtgcgacagg
      181 attatcatta taaatcacgg tcgcatcgtt gcagacagcc ccactgccga gattcgcact
      241 cgtgtgggta atcgctcaac cgtgcgcgtt caaatggctg gctccaaagc ggcaagtcac
      301 ctgtcggccc tgcaaaggct ttccggggtg gcctcggtat cctcgttcga gggagagagt
      361 gttcgggacg cccagggaaa agaaaagcgg ctctgtgggt acgtcctttc ggtaactgac
      421 gataaagatc cccggccgga agtgtttgcc cttgcccggg ataataattg gataatctat
      481 gaaatggctt tggaaaaaaa cagcctggaa gatgtgttta gaaccttaac tatcggagga
      541 gatgacgaat gaaatcacca cgtcctgcat cccttgttat cgcccgccgc gaattggccg
      601 cctatttttc cggcccgatt gcctacatcg taagcggcct ttttttggcc tttaccggct
      661 ttctcttttt ctctacgttt tttttggtta atcgcgccga gcttcgcggc ttctttgcct
      721 tgttgccgat tatgttcgcg ttttttgtcc cggcccttac catgcgtctt ttttcggaag
      781 agtggcgaag cggcagcatc gaaaccctgc ttaccttacc ggtaagctcc ctggatgtga
      841 gccttggaaa gtttgccgcg gcgttttgct ttattgtgtg catgctcctg ccgacgcttt
      901 cgtatgttat taccgccacc gttttaggcg ctccggacgc aggcccaata atcggcggct
      961 atctgggggc tttgtttttg gcggcgagct tttccgctat aggccttttt gcctcttcgc
     1021 ttactgcgaa ccagatagtg gcctttttta tcgccttttc catttccatc gcccttgcct
     1081 tggtggatat ctttttggtt atactccccg cgcccgtcgt cggttttttg caatttattt
     1141 ccgcaggtgc gcactttcag tctatctctc gaggcatcct tgacagtcgt gatcttcttt
     1201 attttgtttc ccttactgcc gcctttttgg cgttaacggt caagaccctt gacctgagga
     1261 gggcaggatg aaaaagaaac tgacttcact atgggcatgg atatgcggac ccaaaagcga
     1321 tgtagcgctt tttttggtcg ctcttgttct tcttaatctt gtggccggcc gtgcctttgt
     1381 tcgcattgac cttactgctc aagggtcgta ctccctgtcc cgctccagcg tggaagtaat
     1441 caagagcctg gatgaaccgc tagccatcaa ggtgtttttt accgaaaaac tccctgctcc
     1501 ctataactcg gtagaacggt atcttcgcga ccttctggtt gaatactccg gcgtgggcag
     1561 caaaaagttc agcttcgagt tttttgacat ggacaaggaa gaaaaccaaa agattgcttc
     1621 ctcctacgga ctttcccttg tacaggtaga agaatacaag gataacgaag ttggcattaa
     1681 aaacgggtgg atggggctgg ctatagtgta tagagacagg atcgaaacac tggacagact
     1741 acaaactccc gagggtcttg aatatcgcat taccacgacg atcagccgga tgatcgcaac
     1801 aacatcatct cttgcgggcc tctctgat
//
LOCUS       DAOQXQ010000390         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_76691_length_1826_cov_2.588933, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000390 DAOQXQ010000000
VERSION     DAOQXQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76691_length_1826_cov_2.588933"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKH81_06710"
     CDS             <1..584
                     /locus_tag="PKH81_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97763.1"
                     /translation="SCALVGGSVSQKELAAEYVAVGDAWADMGKYDKAIGWYEKASQR
                     KEFKNVTRYALGRMYALSGKWEEAVPRFKVLYEEEPDNYLVSQAYAYSLVMIGDTEKA
                     FPIYEKNWKDNSSDPTANRNYAEILFLAGRYEDTLDQIAILREDFSETDVFKGLDELE
                     KKTVDAQKIEIEGEETEYTYEEIKDSAFTRDGL"
     gene            complement(579..1769)
                     /gene="thiI"
                     /locus_tag="PKH81_06715"
     CDS             complement(579..1769)
                     /gene="thiI"
                     /locus_tag="PKH81_06715"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNQ97764.1"
                     /translation="MNIDGKNTAVYLAKLGEIMLKGGNIKDFEQRLVQNAQLFLEGKH
                     CKVLLRNGRMYIEGPLDCAQDIEYTLAHLVAITGWARARVVEKDMCAIKEAVVLEAEA
                     AAKRGCKSFKIEGRRSDKSFPLTSYQIARDCGEVVYESGLLAVDVHNPDSLIMVEVRE
                     RCFVYGVQQSGNRGLPCGTGGKGLLLLSGGIDSPVAGYRMIRRGMKIDCLYFHSHPYT
                     SPEARQKVETLAGILARYGMGTHLNVVPYTEVQQHIRDKAPVDWATVILRMCMMETAN
                     RLASLTGAKALITGESLGQVASQTVENLSVTNSRSKFPVLRPLIGMDKEEIVDTAKAI
                     GTYETSILPFEDCCVLFSPRHPVLRADHEEAARIWTELNIEGLLDKAFEEREHTRIPI
                     IRSL"
BASE COUNT          494 a          423 c          392 g          517 t
ORIGIN      
        1 cctcttgtgc cctcgttgga ggctctgttt cacagaaaga acttgcagct gaatatgtcg
       61 cggtgggcga tgcttgggct gatatgggta aatatgataa agcaataggt tggtacgaga
      121 aggcttcaca acgtaaagaa tttaaaaatg ttacccgtta tgcgcttggc aggatgtacg
      181 cactatcagg taagtgggaa gaggcagttc cacgattcaa ggtgttatac gaagaagagc
      241 ccgataacta tctggtttca caggcatatg cgtattctct ggttatgatt ggagataccg
      301 agaaggcctt tcccatctat gaaaaaaact ggaaagacaa ttcttctgat ccaacagcca
      361 acagaaatta tgcggaaata ctttttcttg ccggtcgcta cgaagatact ctggatcaga
      421 tcgcaatact ccgggaagac ttttcggaaa ctgacgtatt taagggtctt gatgaacttg
      481 aaaagaagac tgttgacgcc caaaagatcg agattgaggg cgaggaaacc gagtatacct
      541 acgaagaaat caaggattct gcctttactc gcgacggctt ataacgagcg gattatcggg
      601 attcgtgtat gctcccgctc ttcaaacgcc ttatccaata atccttcaat attcaactct
      661 gtccatattc tcgcagcttc ttcatgatca gctcgcaata ccgggtgtcg gggagaaaat
      721 agaacacagc aatcttcaaa tggaagaata gaggtctcgt aggttcctat ggcttttgcg
      781 gtatccacga tttcttcctt gtccatgccg ataaggggcc taagtaccgg gaatttgctg
      841 cgactattag ttacggaaag attttcaact gtttgacttg caacctgacc aagactttct
      901 ccagtgataa gggctttcgc tccagtgaga gaggcaagtc gatttgctgt ttccatcatg
      961 cacattctca ggataactgt tgcccaatca acaggggcct tatctctaat gtgttgttgt
     1021 acctctgtgt acgggacaac attcagatgt gtccccatcc cgtatcgggc gagtatacct
     1081 gcaagggttt ctactttttg acgcgcttca ggtgaggtat aggggtgaga atgaaagtac
     1141 aagcaatcta ttttcattcc acgcctaatc atacgatagc cggctaccgg tgaatcaatt
     1201 ccgcctgaga gtaagaggag ccccttgcca cctgttccac acggtaaacc gcgattaccg
     1261 ctttgctgaa ctccatatac aaaacagcgc tcccgaactt ctaccatgat aagtgaatcc
     1321 ggattatgta cgtctactgc aagaagtcca ctctcgtata caacttctcc gcaatctcgt
     1381 gcaatttggt aggaggtgag gggaaaagac ttgtccgatc ttcttccttc aattttaaag
     1441 cttttacatc ctcgttttgc agcagcttca gcttcaagaa caaccgcttc ctttattgcg
     1501 cacatatcct tttctaccac tctggctcga gcccatccgg taatggccac cagatgtgcc
     1561 aatgtgtatt caatatcctg agcgcaatct aggggtccct ctatatacat tctgccgtta
     1621 cgcaataaaa ccttacaatg cttaccttcc aaaaaaagtt gtgcgttttg aacaagacgc
     1681 tgctcaaaat cttttatatt tccaccctta agcataatct cgccaagttt tgcaagatat
     1741 accgcagtat ttttcccatc tatgttcatc aattgctcct tgaaatttct tttaggactt
     1801 tcccaaaaaa gaatagttgt tcatgg
//
LOCUS       DAOQXQ010000391         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_76893_length_1823_cov_2.876131, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000391 DAOQXQ010000000
VERSION     DAOQXQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76893_length_1823_cov_2.876131"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="rplP"
                     /locus_tag="PKH81_06720"
     CDS             <1..361
                     /gene="rplP"
                     /locus_tag="PKH81_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ97765.1"
                     /translation="NATRGNNIDFGEFALVSLEPFWLTNRQLEAARVALNRKVKRGGK
                     LWVRVFPDKPFTKKPAETRMGKGKGAPEYWVAVVKPGTILFELAGIDRELAYAAMHLA
                     GSKLPFKTKFAERLAVE"
     gene            374..580
                     /gene="rpmC"
                     /locus_tag="PKH81_06725"
     CDS             374..580
                     /gene="rpmC"
                     /locus_tag="PKH81_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ97766.1"
                     /translation="MKKKELKSLSHAELVSRRDELNRKYMDLRFQLVIGHVDNPLQKR
                     NMRREIAAVNTLIRQNELSGQGNK"
     gene            552..860
                     /gene="rpsQ"
                     /locus_tag="PKH81_06730"
     CDS             552..860
                     /gene="rpsQ"
                     /locus_tag="PKH81_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNQ97767.1"
                     /translation="MNCPDRETSRSRPVEEQAIKKTGNREFVGVVTSDKMDKTIVVQV
                     STKKLHPLYKKYVSRSKKYKAHDEKNEAKLGDTVRIVESRPISREKCWRLVEIVTRAK
                     "
     gene            875..1243
                     /gene="rplN"
                     /locus_tag="PKH81_06735"
     CDS             875..1243
                     /gene="rplN"
                     /locus_tag="PKH81_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ97768.1"
                     /translation="MIQMQSRLNVADNSGAKLVECIKVPGGSHRRYASIGDIIVVAVK
                     DTLPTSTIKKGSVEKAVIVRISKEYRRPDGTYIRFDDNACVIIDANKNPKGKRIFGPV
                     ARELRDMDYMKIVSLAPEVL"
     gene            1256..1570
                     /gene="rplX"
                     /locus_tag="PKH81_06740"
     CDS             1256..1570
                     /gene="rplX"
                     /locus_tag="PKH81_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ97769.1"
                     /translation="MEKKFKIRKDDTVEIIAGKDKGKRGAVVRVLRDKDRVIVAGANL
                     VKKAMKRRSQQDRGGIVEIEAPLHVSNVMIVCKKCGPVRIGYKIDGDKKVRVCRKCGE
                     AL"
     gene            1570..>1823
                     /gene="rplE"
                     /locus_tag="PKH81_06745"
     CDS             1570..>1823
                     /gene="rplE"
                     /locus_tag="PKH81_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ97770.1"
                     /translation="MSKYIPRLKKVYVEKIAPELFKELGYKSVMQTPKLTKIVLSMGI
                     GEALANKKLLDAAATDLGLITGQKAVKTKAKKSIANFKLR"
BASE COUNT          524 a          404 c          493 g          402 t
ORIGIN      
        1 aaacgcaacc agaggaaaca acatcgattt tggtgagttt gctcttgtgt cccttgagcc
       61 tttttggctt accaacaggc agcttgaagc agcccgtgtc gccttgaacc gtaaggttaa
      121 gcgtggcgga aagctttggg ttcgcgtgtt cccggacaaa ccttttacca agaaacctgc
      181 ggaaactcgc atgggtaagg gaaagggtgc tcctgagtac tgggttgccg tcgtgaagcc
      241 gggaactatc ctttttgagt tggccggtat tgaccgtgaa cttgcttatg ctgcgatgca
      301 cctcgctggc agcaagctcc cattcaagac caagtttgcc gaacggctcg ccgttgaata
      361 agggaggcgc cagatgaaga agaaagagct gaaaagcttg tctcacgccg aactcgtttc
      421 aaggcgcgat gaattgaaca ggaaatacat ggatctccgc tttcagctgg tgatcggtca
      481 tgtagataat cccctgcaaa agcgtaatat gcgtcgcgag atcgcggctg ttaatacgct
      541 catccggcaa aatgaattgt ccggacaggg aaacaagtag gagcaggccc gtggaagagc
      601 aagctatcaa gaaaaccggg aaccgcgagt ttgttggcgt tgttaccagc gataagatgg
      661 ataaaaccat cgtagtgcag gtaagcacca agaagcttca cccactgtac aagaagtacg
      721 tgtcgcgcag caagaaatac aaggctcacg acgaaaagaa cgaagccaaa cttggtgata
      781 ccgttcgtat cgtagagtca aggccgatca gcagagaaaa atgctggcgg ctcgtagaaa
      841 ttgtaacccg ggcaaagtaa acgaggaacc ctagatgatt cagatgcaat caaggttgaa
      901 tgttgccgat aactcgggtg cgaaactggt cgagtgtatt aaggttcccg gtggttctca
      961 ccgccggtat gccagtatcg gtgatattat cgttgtagcg gttaaggata cccttccgac
     1021 ctctacaatt aagaaaggtt cagtggaaaa agccgttatc gttcggattt ccaaggaata
     1081 tcgtcgcccc gacgggactt acatccgctt tgatgataac gcgtgtgtta ttatcgatgc
     1141 taataagaac cccaaaggca agcgcatttt cgggcccgtg gctcgcgagc tgcgcgatat
     1201 ggactacatg aagatcgtat cgcttgctcc agaagtccta taaggagatt gagtaatgga
     1261 aaagaaattc aagatccgga aggacgacac ggtcgagatt atcgccggca aagataaggg
     1321 aaagcgtggt gcagtggttc gcgtcctcag ggacaaagac cgcgtaattg ttgccggagc
     1381 taaccttgtt aaaaaggcca tgaagagacg cagtcagcaa gaccgcggcg gtatcgtcga
     1441 gattgaagcc ccgcttcatg tttccaatgt tatgatcgtc tgcaaaaaat gtggaccggt
     1501 tcgcattgga tacaagattg atggcgacaa gaaggtacgc gtctgccgga aatgtggaga
     1561 agcactgtaa tgagtaagta tataccccgg cttaagaaag tctatgtgga aaaaatcgcc
     1621 cccgaactct ttaaagagct cggatacaag tctgtgatgc agactcccaa actcaccaag
     1681 attgtgttga gtatgggtat tggcgaagct ctcgcaaata agaaactcct tgatgccgca
     1741 gccaccgacc tgggtttgat cacgggacaa aaagctgtaa aaacaaaggc aaaaaagagt
     1801 atagcgaact tcaagcttcg tca
//
LOCUS       DAOQXQ010000392         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77062_length_1821_cov_2.707814, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000392 DAOQXQ010000000
VERSION     DAOQXQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77062_length_1821_cov_2.707814"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKH81_06750"
     CDS             complement(<1..231)
                     /locus_tag="PKH81_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ97771.1"
                     /translation="MAQGQQLIKKNLDFINILPSWAQELSVKYCSKTANLYFVHGNIR
                     DFLPHKMNEGEFMFVRIQDYISEVLFGNRDIIV"
     gene            373..543
                     /locus_tag="PKH81_06755"
     CDS             373..543
                     /locus_tag="PKH81_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ97772.1"
                     /translation="MAYKISDACINCGACEGECPVSAISEKDDKRVIDAATCISCGAC
                     SAVCPVEAISEE"
     gene            629..1048
                     /locus_tag="PKH81_06760"
     CDS             629..1048
                     /locus_tag="PKH81_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97773.1"
                     /translation="MIRNILAGYTDLLQSLLRFVALFAVSLGLGALVVWPLWRLAVGN
                     PQVYPLASAVFATLIIVSIIAVRLRRAYIRDKGLLYTSLIRRLIVVVGVSTSVFFVMH
                     YQRLIALACVFLTLILYGMVAFGINPRRRSSPKNPQL"
     gene            1051..>1821
                     /locus_tag="PKH81_06765"
     CDS             1051..>1821
                     /locus_tag="PKH81_06765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ97774.1"
                     /translation="MNATPGGIRIFLFMSCALWSIASLHLETKPSRYPLIPDLSSLNP
                     VFRQYSDDVSAARIALAGADAKLPLGIYSYRVKSGETLISIAARCSIPYDSIAGLNRI
                     PSATAVLEGKTLLLPTLPGLYLPDQASSDFEHLLLSSFDPASPLILSFTVYDPHTSSA
                     RPVHCIPAQQWDGTVRAFFLKPYFTFPLPQGVLTSSFGYRKNPITGNLIFHKGVDLAA
                     PRGTPVLACRDGTVIVSSFDPVYGNYLILRHDGGRESLY"
BASE COUNT          386 a          515 c          388 g          532 t
ORIGIN      
        1 gacaatgata tcccgattac caaaaaggac ctccgagata taatcttgaa tacggacgaa
       61 cataaactcg ccctcgttca tcttatgggg taaaaaatca cggatattcc catgcacaaa
      121 atagagattt gcggttttgg aacaatattt taccgacaat tcctgtgccc aggatggcaa
      181 tatattgata aaatcgagat ttttcttgat gagctgctga ccctgtgcca tattcccctc
      241 cacccgtcgt cagtgtagca ttactgttac gctaacacaa tattttccgt ttcgagtttt
      301 ttttcacgat atcttgtttt ttctttgccg atgcgttagg gtacaaccat actacttacg
      361 aaggagtctt atatggctta taaaatttcc gacgcctgca tcaattgcgg agcttgcgaa
      421 ggtgaatgcc cggtaagcgc catcagcgaa aaagacgata agcgcgttat tgacgcagca
      481 acttgtatca gctgtggcgc ctgttcagcg gtatgcccgg ttgaggcaat ctccgaggaa
      541 taatcggtat cacacaccgt gcaccgtagc agttgactgc tacggtattt ttttttacgg
      601 acagccctgt tccgtaaggg gagtcgccat gatacgcaac atactcgcag gctacacaga
      661 cttgctacaa tcccttcttc gttttgtcgc cctttttgcg gtctctctgg gcttgggagc
      721 tcttgtggta tggcccctgt ggcgacttgc cgttgggaat ccccaggtat accccctggc
      781 atccgctgtg ttcgccacgc tcattattgt ttccattatc gccgttcgtc tcaggcgggc
      841 gtatatccgg gataaaggtc ttttatacac cagcctaatc cgacgcctga ttgttgtagt
      901 cggtgtgtcc acctctgttt tttttgtcat gcactaccag cgtcttatcg cccttgcctg
      961 cgtgtttctt acccttatcc tctacggcat ggtcgctttt ggcattaacc cccgccgccg
     1021 ttcatcacca aagaatcctc agttgtaacc atgaacgcaa ctccaggcgg catacgaata
     1081 ttccttttta tgtcatgcgc cctctggagc attgcctctt tgcatcttga aacaaagcca
     1141 tcccgctatc ccctgattcc cgatctatct tcccttaatc cggtatttcg ccaatactcg
     1201 gacgatgtct ctgcggccag gatagccctg gcgggcgccg atgcaaaact cccgcttgga
     1261 atctattcct accgggtcaa gagcggagaa accctaattt ctatcgctgc cagatgctcc
     1321 attccctacg actccatcgc ggggcttaat cgaattccgt cggcaaccgc tgtactggaa
     1381 ggaaaaacgc tcttgttgcc cacccttccc ggcctctatc ttcctgacca ggcaagctcc
     1441 gattttgaac acttgctgct ttcttcgttt gatcctgcca gtccccttat cttgtccttt
     1501 acagtctatg atccccacac ttcatcagct cgccccgtcc attgtattcc cgcacaacag
     1561 tgggacggta ccgtgcgcgc ttttttcctt aaaccctact ttacctttcc ccttccgcaa
     1621 ggcgtgctta cctcttcttt tggctaccga aaaaacccga taacggggaa tcttatcttt
     1681 cataaggggg tggatcttgc ggcccctcgg ggaacgccgg tacttgcctg tagagacgga
     1741 acggttattg tctcttcttt tgaccctgtc tatggcaatt atcttattct ccgccatgat
     1801 ggcggacgcg aaagcctcta t
//
LOCUS       DAOQXQ010000393         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77129_length_1820_cov_2.720680, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000393 DAOQXQ010000000
VERSION     DAOQXQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77129_length_1820_cov_2.720680"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKH81_06770"
     CDS             <1..312
                     /locus_tag="PKH81_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ97775.1"
                     /translation="VSSMKIVFWALMFGFGVFWICVVANSFPMLWQMAQFSNIGVYTG
                     LYYTFSQLAAIVAPACSGAIIDLAGYRSMFVFCSVFFLLACFTMGLVTSGEKDDKPVE
                     V"
     gene            complement(309..851)
                     /gene="cyaB"
                     /locus_tag="PKH81_06775"
     CDS             complement(309..851)
                     /gene="cyaB"
                     /locus_tag="PKH81_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNQ97776.1"
                     /translation="MTEIEIKAWVDNPEETEKVIRSFAVYTKELHKKDVYWAYEKPDT
                     DGFIKVRIREDNEGVTVTYKRKEVRGAIEVNDEKEFSISARKDFEVLLADIGFAPYTT
                     KEKQTRVFASTTEGGLPVSIELSYIPPLGWFIELELLANNPDEAFLIRAEAELKAELR
                     RCGIPEEKIEKRFYTELLQG"
     gene            complement(953..>1820)
                     /locus_tag="PKH81_06780"
     CDS             complement(953..>1820)
                     /locus_tag="PKH81_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNQ97777.1"
                     /translation="PIGTIEDGDSVIYFNFRGDRALELTAAFETKDGFDKFDRVRVPK
                     CEYAGMMEYDGDLHIPAQYLVTPPAIDRTMGEYLAASGVKSLAISETQKYGHVTYFFN
                     GNKLGKFDETLEDYVEVKSDVVPFEQRPWMKCAEITDKVIEAIQSGKYDFIRLNFPNG
                     DMVGHTGIYQAVLCSMEGMDLQLARLKKAVLAAGGIMVMSADHGNADDMFEHEKKTGA
                     VSRKEDGSPKAKTSHSLNPVPCIVFDPASKGEYKSALKEGLGISSLAATCIEMLGFKA
                     PADYDSSVLDWN"
BASE COUNT          417 a          442 c          410 g          551 t
ORIGIN      
        1 gtttcttcca tgaagattgt cttttgggcg cttatgtttg gctttggcgt attctggatc
       61 tgtgtcgtag ctaattcatt cccaatgctg tggcaaatgg cacagtttag taacattggc
      121 gtgtataccg gcttgtatta taccttttcg cagctcgcgg caattgtcgc acccgcttgt
      181 tcaggcgcaa taatcgatct tgccgggtac agatccatgt tcgttttttg ttcagttttc
      241 tttttgcttg cctgttttac catgggcttg gttacaagcg gagaaaagga cgataagccg
      301 gtcgaggttt aaccttggag taactcggtg taaaaccgct tttctatttt ttcttccggg
      361 attccgcatc ggcgtagctc ggcttttagt tctgcttctg cccgtatcag gaatgcctca
      421 tcggggttat tcgccaatag ttccagttca ataaaccagc caaggggagg gatgtacgaa
      481 agctctatgg aaacgggtaa gcctccttcc gtcgttgacg caaagaccct ggtttgcttt
      541 tccttggttg tgtatggtgc aaagccaatg tcggcaagaa ggacttcaaa atcctttcgc
      601 gcggaaatcg agaactcctt ttcgtcgttt acctcgatgg ctcctcttac ttcctttcgc
      661 ttataggtaa cggtaactcc ctcattatcc tcccttatgc gcacttttat aaaaccgtct
      721 gtatcgggtt tttcataagc ccaatacaca tcttttttat gtagttcctt tgtgtatacg
      781 gcaaaggagc gaataacctt ttcggtttct tcaggattat ctacccatgc cttaatttca
      841 atctcggtca tattatccct ttgtgtaacg cgagcggtac ttttaaaaaa tcccccgctt
      901 tccaggcggg ggatgtgcag ttcatacaag ttaggctata gcgctactca tcttagttcc
      961 aatctagaac ggaagaatcg tagtctgccg gtgccttgaa tccaagcatc tcgatgcagg
     1021 tagcagcaag gctggaaatg ccaagccctt ctttaagagc gctcttgtat tcgcccttgg
     1081 aagcgggatc gaatacgatg cagggaacag gattcaagga atggcttgtc tttgccttgg
     1141 gcgatccgtc ctctttacgg gaaacagcgc cggttttctt ttcgtgctca aacatatcgt
     1201 cggcattgcc gtgatcggcg ctcataacca taatgccgcc tgctgcaaga acggccttct
     1261 tgagccgtgc aagctgaaga tccatacctt ccatggaaca gagtactgcc tgataaatac
     1321 cggtgtgtcc taccatatct ccattgggga aattaagacg gataaagtca tactttcccg
     1381 attgaatggc ttctataacc ttatcggtaa tctccgcaca cttcatccat ggcctctgtt
     1441 caaagggcac aacatcgctt tttacttcaa catagtcttc cagtgtttcg tcaaactttc
     1501 caagcttgtt accgttaaaa aagtaggtaa cgtgtccata cttttgtgtt tcgcttatcg
     1561 caagagactt tactccggaa gccgcaaggt attcacccat ggtccgatca atcgcgggcg
     1621 gggtaaccag atactgcgcg ggaatatgaa gatcaccgtc atattccatc atgcctgcgt
     1681 actcgcactt gggtacacgc acacgatcga atttatcaaa gccgtctttg gtttcaaagg
     1741 ctgccgtcaa ttcaagggca cggtctccac ggaagttaaa atagataaca gagtctccat
     1801 cttcgattgt tccgatgggt
//
LOCUS       DAOQXQ010000394         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77513_length_1815_cov_2.310227, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000394 DAOQXQ010000000
VERSION     DAOQXQ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77513_length_1815_cov_2.310227"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..375
                     /locus_tag="PKH81_06785"
     CDS             115..375
                     /locus_tag="PKH81_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97778.1"
                     /translation="MKPISINATLPPESFALVLTGEKRFIRHKRNPRIDRYFLSKIPT
                     IANIRCNGETLPCQITRIEQTPERWIIHIKRGAYRGPSRVSG"
     gene            complement(404..1585)
                     /locus_tag="PKH81_06790"
     CDS             complement(404..1585)
                     /locus_tag="PKH81_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97779.1"
                     /translation="MLETFIGGYLFHPAALDYSGPTCTNGCVYCFANINKSERKASLK
                     QAITCLYKKEATTYRDLLVKLGYPICVSNRSDPFSRNNARETAQLFSCLSEFSNGIFI
                     QTKCGPGMDEAIVALNGKSNVVWYITVTTMRDDLSKQIEPFAPASSERLAWAKKLHDM
                     GYLVVIAVNPCTDIWMPMSDLQDLMRYMKGAGLNHLVVEMLDLKAKRVQKMSERTKAN
                     IGNAAIDSCLSGRCRLYVRECMDEIHGSGLALAKKGMPYRSEFFNDLKRVLGVTMPVF
                     QDFIHYCFDRYATGGPITFDEFASVITDDRIFKMTVRQNNIRDYLLRAGFLSWKDNQQ
                     VHTHRDLLRIIWNDSRHRLSIHNHCLIRSTNRQDPEGNAILWFTATPIFDKKRKEVMQ
                     L"
     gene            complement(1587..>1815)
                     /locus_tag="PKH81_06795"
     CDS             complement(1587..>1815)
                     /locus_tag="PKH81_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97780.1"
                     /translation="LSWTPGDDRSKMRKNFNDGCGAYDISGVADSSAQADRYPVTVVL
                     DADEYERWQAAKERLGVKDDKKALLKLIGGN"
BASE COUNT          426 a          494 c          467 g          428 t
ORIGIN      
        1 cgatgcgggc gctaaagccc tagcgtacct gcaagaacta cccggagggt ttaacttctc
       61 ccggtggctg cgggacgcgc tggtggagtt ggcaagaaag agagggtgga gggaatgaaa
      121 ccgatctcaa tcaacgcaac gcttccaccc gagtcgttcg ccctggtgct gaccggggag
      181 aagcgtttca tccgccacaa gcgcaacccg cgcatcgaca gatattttct gtcaaaaatc
      241 cctaccattg caaacatccg ctgcaacgga gaaacattgc cctgccaaat cacccgcatt
      301 gagcaaacgc cggaacgatg gataatccac ataaaaagag gggcctatcg tggcccctct
      361 cgggtgagtg gttaagccca ctcgccgccc cagaagttgg tcttcataac tgcatcacct
      421 ccttccgctt cttgtcaaaa atcggggtag ctgtaaacca caagatagcg tttccttccg
      481 gatcttgccg gtttgtcgaa cgaatcaagc agtgattatg gatgctcagc cgatgtcgcg
      541 aatcattcca aataatccgg agcagatcgc ggtgggtatg cacctgctgg ttatccttcc
      601 aggacaaaaa tccggcccga agtaggtaat cgcggatatt gttctggcgc accgtcatct
      661 tgaaaatgcg gtcatcggta ataactgaag cgaattcatc aaaggtgatc ggcccgccgg
      721 tggcgtagcg atcgaagcag tagtggataa agtcctgaaa caccggcata gtgacaccta
      781 agacgcgctt caggtcgttg aaaaactcgc ttcggtatgg cattcctttc ttcgccaagg
      841 caaggccgct tccatggatc tcatccatgc actcccgaac atacaggcgg catcgcccgg
      901 acagacatga atctatagct gcgttcccaa tgttcgcctt tgtgcgctcg ctcatttttt
      961 gaacccgctt tgccttcaaa tcgagcattt caacaaccag gtgattcaat cccgcgcctt
     1021 tcatgtaccg catgaggtct tgtaagtcgc tcattggcat ccagatatcg gtgcaaggat
     1081 tgacagcaat caccacgaga tagcccatgt cgtgcaactt cttcgcccag gccaagcgct
     1141 cagacgaagc gggcgcgaag ggctcaatct gctttgatag gtcatcccgc atagttgtga
     1201 ccgtgatata ccagacgaca ttgcttttgc cgttaagtgc cacaattgcc tcgtccatgc
     1261 cagggccgca cttggtctgt ataaagatcc cgttcgagaa ctctgaaagg caggaaaaga
     1321 gctgtgccgt ctcacgggca ttgttacggg agaacgggtc agagcgatta gaaacgcaaa
     1381 tcggataccc gagcttgacc agtagatccc ggtatgtggt ggcctctttc ttatagaggc
     1441 aggttattgc ctgcttgagg cttgctttgc gctcgctttt gttgatgttc gcgaaacagt
     1501 agacacagcc gtttgtgcag gtaggcccgg agtaatcgag cgccgcagga tgaaacaggt
     1561 atccgccgat gaatgtttca agcatgtcaa tttcctccta tcagcttgag caatgccttt
     1621 ttgtcgtctt ttacgccgag cctttccttt gccgcttgcc agcgttcata ctcgtcggca
     1681 tcgagtacaa ccgtcaccgg gtagcggtcg gcttgagcgc ttgagtcggc gacgccagaa
     1741 atatcataag ccccgcatcc atcgttgaag ttctttcgca ttttgcttcg atcgtcgcca
     1801 ggcgtccagg aaagc
//
LOCUS       DAOQXQ010000395         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77712_length_1812_cov_2.634604, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000395 DAOQXQ010000000
VERSION     DAOQXQ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77712_length_1812_cov_2.634604"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /gene="rho"
                     /locus_tag="PKH81_06800"
     CDS             complement(<1..1488)
                     /gene="rho"
                     /locus_tag="PKH81_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ97781.1"
                     /translation="MAPIRRRRTPETPEGTTQETAEQAQLELGGEEGRSEPVMEYSES
                     AEETSSVSQETTIPDNGDKRVVVRPRIKRKPGPRDQNGRENGHREYRDNRRFRDQGYE
                     TREARESREEGREIQMLQDAEAARDNENKPRLTINELTRMGMHALRDLATQYGVPHED
                     MVAMKKQEIIFQILKGHTEHGGIIFASGSLEILPDGYGFLRSPQNSYLPGPDDIYISP
                     SQIRLFNLKTGDTVYGQIRSPKEGERFFALLRVETVNFDEVAAAQSRIPFDNLTPLYP
                     NEKIDLETTTTEFSTRIMNLFCPIGKGQRALIVAPPRTGKTIIMQKIANAITANHPEV
                     YLIVLLIDERPEEVTDMERTVKAEVISSTFDEQATRHVQVAEMVLEKARRLVEHKRDV
                     VILLDSITRLARAYNQTVPTSGKVLSGGVDSNALHKPKRFFGAARNVEEGGSLTIIAT
                     ALIETGSRMDEVIFEEFKGTGNMEINLDRKLSDRRLFPAVSIKKSG"
     gene            complement(1597..>1812)
                     /locus_tag="PKH81_06805"
     CDS             complement(1597..>1812)
                     /locus_tag="PKH81_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNQ97782.1"
                     /translation="LAVTGFAGPTGGTPENPVGTVYIACACCANESLAGDYPVRVRRI
                     EVCGSRERVRRVTVQAALLLALQTLDS"
BASE COUNT          420 a          453 c          435 g          504 t
ORIGIN      
        1 accgcttttc ttgatgctta cggcaggaaa caggcgccga tcagagagtt tacggtcaag
       61 attgatttcc atattaccgg ttcccttgaa ttcctcaaag ataacctcgt ccatacggct
      121 tcctgtttct atcagagctg tcgcgataat agtaagactt ccgccttcct caacgttacg
      181 agcagcacca aaaaaacgct tgggtttatg aagggcgtta gaatcaacac cacctgaaag
      241 aaccttacca gaagtcggaa cagtttggtt ataggcgcgg gccagacggg taatagagtc
      301 caaaaggatt acgacatccc gcttgtgctc taccagacga cgagcctttt ccaaaaccat
      361 ctccgctacc tggacatggc gggttgcttg ttcatcaaat gtagaagaga ttacttccgc
      421 tttaactgtc ctttccatat cggttacttc ttccggccgc tcgtctatca acaagacgat
      481 caggtatacc tcgggatggt ttgcggtaat cgcgttggcg attttttgca taataatcgt
      541 tttaccagta cgggggggag ccacgattag agcgcgctgc cccttaccaa tcggacagaa
      601 aagattcatg atacgggtag aaaactcggt agttgtcgtt tccagatcaa tcttttcgtt
      661 gggatacaga ggggtcaggt tgtcaaaggg tatcctgctt tgtgcggcag caacctcatc
      721 aaagttgact gtttcaaccc gcaacagggc aaagaagcgc tcaccctctt tgggagagcg
      781 aatctgacca tacacagtat cgccagtttt caggttaaaa agacgtattt ggctggggga
      841 gatataaata tcatcaggac caggcaaata actgttttgg ggagatcgaa gaaacccata
      901 tccatcagga agtatttcta aagaaccgga ggcaaaaata atccctccgt gttcagtatg
      961 tccttttaga atctggaaga tgatttcctg ctttttcatg gcgaccatgt cttcgtgcgg
     1021 gacgccatat tgggttgcca gatcgcgcag ggcatgcatt cccatgcggg taagttcgtt
     1081 aatcgtcagt cgtggtttgt tttcgttatc gcgggcggct tcagcatcct gaagcatctg
     1141 tatctctcgc ccttcctctc gtgattctcg ggcttcccga gtttcgtatc cctgatcgcg
     1201 aaatcgccgg ttatcgcggt attcgcggtg tccgttctct ctgccgtttt gatctcttgg
     1261 tccgggcttt ctttttattc gtggccttac taccacgcgt ttgtctccgt tatctggaat
     1321 cgttgtttcc tgactaacgg acgaggtctc ttcggcgctt tccgagtact ccattacggg
     1381 ttcggaacgg ccttcttcac cgccaagctc gagttgagcc tgttcagcgg tttcttgcgt
     1441 cgtaccttcc ggtgtttccg gtgtacggcg ccttctgatt ggtgccatgg gaattctcca
     1501 tatatagatt gcggtgcaaa agaatgaaag gcattatgtg ttcggatttt tcctgaaaaa
     1561 aagataggaa tacaatgagt ataccgcctg cttgtattag ctgtcaagag tttgaagggc
     1621 aagtaacaag gctgcctgga ctgttaccct tctaaccctt tctctggatc cacaaacctc
     1681 aatccgtcgc acgcgaacgg ggtaatcgcc tgccaatgat tcattagcac aacaagcaca
     1741 tgctatatac accgttccca ccggattctc cggtgttcca cccgtcgggc cggcaaaccc
     1801 ggttaccgca ag
//
LOCUS       DAOQXQ010000396         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77841_length_1810_cov_2.668376, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000396 DAOQXQ010000000
VERSION     DAOQXQ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77841_length_1810_cov_2.668376"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKH81_06810"
     CDS             complement(<1..846)
                     /locus_tag="PKH81_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNQ97783.1"
                     /translation="MKKQYTVYAILAATLILPALLTAQKANVVYVEGSSQIKSSSGSL
                     RPADFGSTVTYGESVITGRDGFVEMTLENGSTIRVAENSVFSYSSTGAAADSRPVLAT
                     TAGKVSYKLNKAAGRSPLIQSNSMVAGVRGTEFTVFSACDGSTLLAVTDGIVDVESQG
                     TKIELVKDEAVEVTPGKAPGTKFTWLGKELDYSSWNKGKNDEFIQNPLACLDLVATQL
                     DVYKSALESLKGPYEQATITWQKATDQYKALVAEGDKQAMEAFRASTLYPAQDERSTI
                     ILNIRY"
     gene            complement(843..1307)
                     /locus_tag="PKH81_06815"
     CDS             complement(843..1307)
                     /locus_tag="PKH81_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97784.1"
                     /translation="MKVRILVLPVVLLLCALLASCASGPYVQKEDEVMNLVQLINDGR
                     VSEVEGLTPAPFALDGETLYLENDIATFWKNLKAASFVMKDARFVSSEHVGPDSYKAF
                     APSYDMKNFFARYTGKDTSLTVLETPDGRYYLLLERAIKGYPRIRGLRGPVR"
     gene            complement(1344..>1810)
                     /locus_tag="PKH81_06820"
     CDS             complement(1344..>1810)
                     /locus_tag="PKH81_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97785.1"
                     /translation="LTIEGILTFVLACQILLGEGGRIRFSVRQPARGMRISRLATDST
                     LTKAVGDAEERFFTDPAHKRFSWTDEELTGQLQKKGFTVNKEEIENTELRRPSTQDLE
                     RWFDPSRSTYARYLVEHLGEDTMQRFTGEILERCLKEAVPWNYTIVLYTVSL"
BASE COUNT          415 a          478 c          448 g          469 t
ORIGIN      
        1 atagcggatg ttcaggatga tggtgctcct ttcatcctga gcgggataca aggtggatgc
       61 acgaaacgct tccatcgcct gtttgtctcc ctctgctacc agggccttgt actggtcagt
      121 tgccttttgc caggtaatgg tcgcttgttc gtacggtccc ttaagcgact caagtgcgct
      181 cttgtatacg tcaagctgag tggctaccaa gtcaaggcag gcaagggggt tttgtataaa
      241 ctcatcgttt ttccccttgt tccaggaact gtaatcaagc tccttgccaa gccaggtaaa
      301 cttggtccca ggggctttcc ccggtgttac ttcaaccgct tcgtctttta ccagttcaat
      361 ttttgtaccc tgggactcaa catccacaat accatcggtt acggcaagga gggtggagcc
      421 gtcgcaggcc gaaaataccg taaactccgt tcctctaacg ccggctacca tactgttgga
      481 ttgaataagc ggggatcttc ccgctgcctt gttcagcttg taggaaacct ttcccgcagt
      541 ggtggctaga acaggccgag aatccgcggc tgctccggtg cttgaatagc taaacaccga
      601 attttctgca acccggatgg tgctgccgtt ttccagcgtc atttccacaa aaccgtcccg
      661 tccggtaatc accgattctc cgtaggtaac ggtagagcca aaatccgccg gccgtaaact
      721 gccagaactg gatttaatct gggacgaacc ttctacgtac actacgtttg ccttttgagc
      781 cgtcaacagg gcgggaagaa tcaacgtagc cgccaggatt gcgtatactg tgtattgttt
      841 tttcatcgta ccggccccct tagtccgcgg attcggggat accccttaat cgctcgttcc
      901 aataacagat aataccgtcc gtccggggtt tccagtacag taagggaggt gtcttttccg
      961 gtataccggg caaaaaagtt tttcatgtcg taggagggcg caaaggcctt gtaggaatca
     1021 ggtcccacat gctcgctgga gacaaaccgg gcgtccttca taacaaaaga tgctgccttc
     1081 aagtttttcc aaaaagttgc gatgtcattt tccagataca gggtttcgcc atcaagggcg
     1141 aagggagccg gggttagtcc ctctacttcg gatacccttc cgtcatttat cagctgtacc
     1201 agattcatca cttcatcttc cttttgtaca tagggcccgc tggcgcacga tgccaagagg
     1261 gcgcatagaa gaagaacaac aggaaggaca agaatgcgta ctttcaaaat caaacctcct
     1321 ttatgtattc gccggtaagt atatcacagg ctcacggtat ataacacaat tgtatagttc
     1381 cagggtacag cttccttcaa gcaccgctca agaatttcgc ccgtaaagcg ttgcatcgta
     1441 tcctctccca gatgttctac cagatagcgg gcataggtgc tgcgagaagg gtcaaaccaa
     1501 cgctcaaggt cttgtgtgct tgggcggcga agctcggtgt tttctatttc ctctttgtta
     1561 acggtgaacc ccttcttttg gagttgcccg gtaagctctt catcagtcca ggaaaaacgc
     1621 ttgtgggcag gatcggtaaa aaaccgctct tctgcatccc ccacggcctt ggttaacgtg
     1681 ctgtctgttg caagccgtga aattctcatc ccgcgcgcgg gttggcgaac ggaaaagcga
     1741 atccttccgc cttcgcccaa aaggatctgg caggcgagca caaaggttag tattccctcg
     1801 atggtaagcg
//
LOCUS       DAOQXQ010000397         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_77897_length_1809_cov_3.076397, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000397 DAOQXQ010000000
VERSION     DAOQXQ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77897_length_1809_cov_3.076397"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKH81_06825"
     CDS             complement(<1..500)
                     /locus_tag="PKH81_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ97786.1"
                     /translation="MNNNSIVPGFDEEKDDSLKIRLEKIDDIEGCIALYLNGYIDTYN
                     SSFFQKRVSKIVEAGFIRLIFSCGSLNYVSSTGIGSFTAFLKTVKPKGGDVVLLEIQP
                     KVYEVFQLLGFSQFFNIKDSLADSIAFFKTTGVEIKSEIFPKIFSCPICSKKLKATKA
                     GRFRCS"
     gene            complement(572..1309)
                     /locus_tag="PKH81_06830"
     CDS             complement(572..1309)
                     /locus_tag="PKH81_06830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ97787.1"
                     /translation="MPLVMSILVGYDYRMNGMFSGKKALVVGGTGGIGRAISMALQGA
                     GAEVLAIGGHPLDGTTFLRVDLDSPENCAIVCSKASEADIVCCVRGPFLQKEVAATSV
                     DEWNSVVYANLTLPGILVSAALPRMIERSWGRILLMGGTRTDRIRGFRTNAAYAAAKT
                     GLSSLTRSVAHSYASHGITCNCICPGFVDTEYLDEATKTALAAKNPDGALISATEIAE
                     AGIFLLSHPSCNGICLPIDKGWQPSFG"
     gene            1327..>1809
                     /locus_tag="PKH81_06835"
     CDS             1327..>1809
                     /locus_tag="PKH81_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ97788.1"
                     /translation="MKKLPCAIVLCRPEESRNIGSVCRAMMTMACHDLRIVGNRDEID
                     EGQVKTLAVHAGEVWDNARFFPPGIEGLKAAIQDCALAAGTTRRMGMKRKSWGMTPAQ
                     FAHLATQNHQGLAAVVFGNERTGLTDEELDCCSLAVNIPSNPAFPSLNLSHAVQILTW
                     T"
BASE COUNT          526 a          451 c          398 g          434 t
ORIGIN      
        1 tctgaacacc taaagcgacc agccttggtt gctttaagtt ttttggagca gatggggcaa
       61 gaaaatatct tggggaagat ttctgattta atctcgacgc cagtcgtctt aaaaaaggcg
      121 atagagtctg ctaaggagtc tttaatatta aagaattggg aaaaacccag taactggaat
      181 acttcgtaaa cctttggttg aatttccaaa agaacgacat ctccaccctt gggtttaact
      241 gttttcagga acgccgtaaa agatccaata ccagtagagg acacataatt gagtgaacca
      301 caactaaaaa tcagccgaat aaatcctgct tcaacgattt ttgacactcg cttttgaaaa
      361 aagctcgaat tgtaggtatc gatatagccg ttgaggtaca gggcaatgca tccctcgatg
      421 tcgtcaatct tttccagacg gatttttaga ctatcatcct tttcttcgtc aaatcccgga
      481 acaatgctgt tgttattcat atagctccta ccgcctagag tatcggcatt atctctaata
      541 ctataaacta taaacagaat aaaataaatg gtcaaccaaa agaaggttgc catcctttat
      601 ctatcggcag gcaaattccg ttacatgagg ggtgcgaaag caggaaaatc ccggcttccg
      661 cgatctcggt agcagaaata agcgccccat cagggttttt tgccgccaat gctgttttag
      721 tggcctcgtc caggtactcg gtatccacaa aacccgggca gatacaatta caggtaatac
      781 catgcgaggc atatgagtga gcaaccgaac gcgtaaggga agaaagtccc gtttttgcag
      841 cagcataggc tgcattagtt ctaaaaccac gtatccgatc cgttcgagtt ccgcccatca
      901 acaaaatcct gccccaactc cgctcaatca tgcggggcaa agctgcagat accagaattc
      961 cgggaagagt cagattggca tacacaaccg aattccattc gtccacgctc gtagcagcaa
     1021 cttctttttg taaaaacggc cctcgtacac agcaaacaat atctgcttct gatgccttag
     1081 agcaaactat agcgcaattt tccggactat caagatctac gcgtaaaaaa gttgttccat
     1141 caagaggatg accacctata gccaacacct cggcgcccgc gccttgcaaa gccatagaaa
     1201 tggctcgacc aatccccccg gttccgccaa caaccagggc ctttttacct gaaaacatcc
     1261 cattcatacg ataatcatag ccgaccaata ttgacataac aagcggcatg gcagaaaatc
     1321 ggcaacgtga agaaattacc ctgcgccatt gtgctttgta gaccggaaga aagccggaat
     1381 atcggatcgg tgtgccgggc catgatgact atggcatgcc atgacttgcg cattgtagga
     1441 aacagggacg agattgatga gggacaggtt aagacactgg cggttcacgc gggcgaggta
     1501 tgggacaatg cccgtttctt tccccccggg atagaaggcc ttaaggcggc aattcaggat
     1561 tgcgcccttg ccgcgggaac cacccgaaga atgggtatga agcgtaaatc gtggggaatg
     1621 actccagctc aattcgccca tcttgccaca caaaaccacc aaggacttgc cgcggttgta
     1681 tttggtaatg aacgaacagg cttaaccgac gaggaacttg attgctgttc tctggccgtc
     1741 aatatcccca gtaatcctgc cttcccctcg ttaaaccttt ctcatgccgt acaaatactt
     1801 acctggacc
//
LOCUS       DAOQXQ010000398         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_78046_length_1807_cov_2.472603, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000398 DAOQXQ010000000
VERSION     DAOQXQ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78046_length_1807_cov_2.472603"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKH81_06840"
     CDS             complement(<1..1192)
                     /locus_tag="PKH81_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ97789.1"
                     /translation="MGAWCVDDSKFPGISSEGRQMLYRLSAHPHAPILRDHSGPSLST
                     EDCLSVRDFFTDVERGSPWKSLWSPPDWLTEFVVRTLKVVPYYRSKYPEGAAFDAITP
                     IGRAQARIHVARFVPDDLPVDKLIVFSSSGTTGLPLSVPSHPIVAARYYAFHKRALGR
                     VGIVPCAGAGEVAVVLAGYQKRCFTYASVNPLMDEAGLVKINLHPDEWRHPEDRARYI
                     DSLAPELISGDPLSLAELAQLSITHRPRAVLSTSMALSPATRASFESSFGCPVVNVYS
                     MTEAGPIASWSDRHNGFLVLQNRLYIEILSPEGVPLLPGSRGEITLTGGFNDYFPLLR
                     YRTGDYGWAEESPEGIVLRDVEGRPPVRFRAMNGGWLNNVDVTHALSPFDLVQYALHQ
                     KDNER"
     gene            complement(1199..1780)
                     /locus_tag="PKH81_06845"
     CDS             complement(1199..1780)
                     /locus_tag="PKH81_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97790.1"
                     /translation="MKKIGPPLAHLLRRLEDTPVEFYTLPVNGAPDSMAVHALIADLC
                     LTLGTPFKIKDINRLCVGRGVISDNTYRLMAVGTWFLSDTSLTFLLTPSALYTVIEKS
                     LAQLSYERNVEYYLFEEDGREEFVRAVLRDLGSRPEGETIAQAEDRLSAVSSQARRVV
                     MGEAAEELARDRQLRAALAKKAAEEAADKYMRE"
BASE COUNT          471 a          491 c          471 g          374 t
ORIGIN      
        1 accgctcatt atctttttgg tggagtgcgt attgcacaag gtcgaaagga gacagagcat
       61 gggtaacatc cacattgtta agccacccgc cgttcatggc tctaaaacgc accgggggac
      121 gtccttccac atcacgcaat acaatgcctt ccgggctttc ttccgcccag ccgtaatccc
      181 ctgttcgata gcgaagcaag gggaagtaat cgttaaagcc gccggtcagg gtaatttcgc
      241 cgcgagagcc cgggaggagc ggaactcctt cgggagaaag aatctcgata tataaacgat
      301 tttgaaggac caaaaaacca ttgtgtcggt ccgaccagga tgcgatgggt cctgcttccg
      361 tcattgagta cacgttcacc accggacaac caaacgacga ttcaaagctc gctcgtgtag
      421 cgggagacag cgccatggaa gtggaaagaa ccgcgcgagg gcgatgcgtt atggaaagtt
      481 gagcaagctc ggcaagagaa agcggatccc cggaaataag ctcgggggca agagaatcta
      541 tatagcgggc gcgatcttcg gggtgccgcc actcatccgg atgaaggtta atttttacca
      601 aacccgcctc gtccataagg ggattcaccg aggcataggt aaaacatcgt ttttgatatc
      661 ccgctaggac gacggctacc tctcccgcgc ccgcgcaagg gacaattcca acccggccca
      721 gggcccgctt atggaaggca tagtagcgag cggcaacaat ggggtgggat ggaacagaca
      781 agggtaggcc agtggtgcca cttgaagaaa acacaatgag cttgtctacc ggaagatcat
      841 ccgggacgaa ccgagctacg tgtatgcgcg cttgcgcacg gccaatcggg gtaattgcat
      901 cgaatgccgc gccttctgga tactttgatc ggtaataagg aacaaccttg agtgtccgca
      961 caacaaattc tgtaagccaa tcaggaggtg accagaggct cttccatgga gagcccctct
     1021 ctacgtccgt aaaaaaatcc cgaaccgaca agcaatcttc cgtgctcagg gaagggccgc
     1081 tatgatcgcg caaaatgggt gcatggggat gtgcggaaag acgatagagc atttgtcggc
     1141 cttccgagga gataccggga aatttactgt cgtctacgca ccaggctccc attatctact
     1201 attcccgcat atacttatct gcggcttcct ccgcagcttt ttttgcaagg gctgcgcgaa
     1261 gctgccgatc cctggcaagc tcctccgcgg cctcgcccat tacaacgcgc cgagcctgac
     1321 tgcttaccgc cgagagacga tcttctgcct gtgcgattgt ttcaccctcg gggcgggaac
     1381 ccaaatcacg caacactgcg cgaacgaatt cctctcggcc gtcttcctca aacaaataat
     1441 actccacgtt acgctcatat gataattgcg caagactctt ttcaataaca gtatacaaag
     1501 cggaaggcgt aagcaggaag gttagagatg tatcagataa aaaccaggtc cccacggcca
     1561 ttagtcgata agtattgtca ctgatcactc cacgaccaac gcacaagcgg ttaatatctt
     1621 ttattttaaa tggggtgcca agggtcaggc acagatcggc aatcaaggca tgaacggcca
     1681 tcgaatctgg cgcgccatta accggcaggg tataaaactc cacaggagta tcctcaagcc
     1741 gacgcagcaa atgcgcgagc ggtggtccta tcttcttcat accctgctcc acgtcccgct
     1801 atctata
//
LOCUS       DAOQXQ010000399         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_78196_length_1805_cov_2.450857, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000399 DAOQXQ010000000
VERSION     DAOQXQ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78196_length_1805_cov_2.450857"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..383)
                     /locus_tag="PKH81_06850"
     CDS             complement(51..383)
                     /locus_tag="PKH81_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97791.1"
                     /translation="MSVENRIESEIELDQMTDSREEGKIFCANCIHCKLVSAPAGQTG
                     QFYLRVRCAAGQWKKKLGEEKQYKYFTVTRRSIDYCSKYEPMGEAGEFISDLRKTLPI
                     RDEIYEKD"
     gene            complement(421..1749)
                     /locus_tag="PKH81_06855"
     CDS             complement(421..1749)
                     /locus_tag="PKH81_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ97792.1"
                     /translation="MPKLPIGSLFFLLISLTAVPGQQKPITATSTINWKTETVTSIIR
                     LDTHATGVRLPEGRSSALELIEMKLPSLLQETFYSIPLDSREKLGHAVERGSLSLSEL
                     NKIIDSAKKTPPWFSSDLTSLSMSCTMPLSTLGSIFVTHSKAYEPKAPLQSIHTRAYS
                     GIVIDARGSLPVHGEYREDTVQPCLFPKIWNTDMESVYEKNMMDGKRAQKAGIISYAS
                     ESESAEVLQRVGKDPLRIRARGVFGNHRTDPVISMEDYLKIYALADNRALLKEGKIVI
                     LLDQATLESQPLGVPKDSEYYFAWGEMQKSLETVPVTRIDLSDSWEGLKLTIYDIRFV
                     ADTAEILSEEKSRLDSIADALKRAGPQARFYIEGHTAAVGKPEGERRLSGERAQKIAQ
                     EMETRGIPSDRLEATGFGGTRPIGPNETPEGRARNRRVEIIIHIDGTQVP"
BASE COUNT          406 a          441 c          443 g          515 t
ORIGIN      
        1 gtgagcgctg cgataagcaa ggctccaaaa atattcctta acatgaacgt ttaatccttt
       61 tcgtatattt cgtcccgaat gggaagtgtt ttacgcagat cactgataaa ctcaccggct
      121 tcgcccattg gttcgtattt tgaacaataa tcgatacttc tacgagtaac agtgaaatac
      181 ttgtattgtt tttcctctcc cagcttcttt ttccattggc cggcggcaca acgaacccga
      241 aggtaaaact gaccggtttg accggctgga gcgcttacca gtttgcagtg gatgcaatta
      301 gcgcaaaaga tcttgccttc ttccctggaa tcggtcatct ggtcaagttc gatctcgctt
      361 tcaattcgat tttctacgga catgggataa tccccctttt tttaacgaat ttatagttta
      421 tcacggcacc tgggtgccgt caatgtgtat gattatctct acccgacggt tacgggcacg
      481 gccctccggg gtttcatttg ggccaatggg tcttgttcct ccaaaacctg ttgcttcaag
      541 ccggtcagag gggatacctc ttgtttccat ctcttgggca attttttgcg ctcgctctcc
      601 tgataaacgg cgctctccct ccggttttcc aactgcggca gtatggcctt ctatgtaaaa
      661 gcgggcctgc gggccagctc ttttcagggc atcggctatt gaatcgagcc tggacttttc
      721 ttcgctcagt atctcggctg tatcagcgac aaaacggatg tcataaatgg tcagcttaag
      781 cccttcccag gaatccgaaa ggtctatgcg agtaacggga acggtttcca ggcttttttg
      841 catttctccc caggcaaagt aatactcgct atccttgggt actcccaggg gttggctctc
      901 aagcgttgcc tggtcaagga ggataacaat ctttccttct tttaacaggg cccgattgtc
      961 cgctaaggca tatatcttaa ggtaatcttc catggaaatg actggatcgg tgcgatgatt
     1021 gccaaacacc ccgcgggcgc ggatgcgtaa gggatccttg cccacccgct gcagtacttc
     1081 ggcactttcg ctttccgacg cataactgat aattcctgct ttttgcgcac gtttcccgtc
     1141 catcatgttt ttttcgtaga cggattccat gtcagtattc caaattttgg ggaacaggca
     1201 gggttgcacc gtatcctcac gatattcacc gtgaacgggg aggcttcccc gagcgtcaat
     1261 cacaataccg ctatatgccc tggtatgtat gctttgaagg ggggcttttg gctcatacgc
     1321 cttggaatgg gtaacaaaga tactccccaa ggtggaaagc ggcatagtac agctcatgga
     1381 caggctcgtt aagtctgagg aaaaccaggg cggggttttt ttcgcactat cgataatctt
     1441 gttaagttcg gaaaggctca gggagcctcg ttcgactgca tggcctaact tttcccgtga
     1501 atcaagcgga atagagtaaa aagtctcttg tagcagggat ggaagcttca tctctatcaa
     1561 ctccagggca gaacttcttc cctcgggcag gcgcacgccg gttgcatgag tgtccaggcg
     1621 aataattgac gttacggtct cagttttcca atttatggtt gacgtggcgg taattggttt
     1681 ttgttgcccc gggactgctg tcagggaaat caagagaaaa aagagagaac cgatggggag
     1741 ttttggcatg ataagctcct attcggttac agtatcggca cgcgaagttg ttttcccaac
     1801 ttgag
//
LOCUS       DAOQXQ010000400         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_78662_length_1798_cov_2.837636, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000400 DAOQXQ010000000
VERSION     DAOQXQ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78662_length_1798_cov_2.837636"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKH81_06860"
     CDS             complement(<1..535)
                     /locus_tag="PKH81_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ97793.1"
                     /translation="MGFRYTDYDVIVVGGGHAGIEAALAPARMGLSTLLITQTLDSIG
                     KLSCNPSIGGISKGNIVREIDALGGEMARLADATMIQYRLLNKSRGPAVQAPRVQTDK
                     YRYHLLAKHTLEMQPGLSIYQDTVVALEIAGADESGYVDQGVITAVRTSRGRSISART
                     VVLTTGTFLEGKIFIGEY"
     gene            complement(574..1191)
                     /locus_tag="PKH81_06865"
     CDS             complement(574..1191)
                     /locus_tag="PKH81_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ97794.1"
                     /translation="MSLELISVVKHWPMKDIFLSFTLHTGESLALLGPSGCGKSSVLR
                     IIAGLLPPDSGRVVIGGRDVTALSPGKRGVGMVFQESALFPHLSVEDNIGYGPRSAGK
                     TKKESRQIAAFWLERFSLSGFARRNVDSLSGGERQRVSLARTLAVEPAVVLFDEALSA
                     LDQELRVRLGSELRERQRELGYTAVYVTHDREEAQRLAERVLVME"
     gene            complement(1188..>1798)
                     /locus_tag="PKH81_06870"
     CDS             complement(1188..>1798)
                     /locus_tag="PKH81_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ97795.1"
                     /translation="LFSRPSFFRSLGTTVWVSSCAAFLSTIAALALTLLVGRSRSAVT
                     RVLPLVPLAVSSVMLGFGWIVLAPRMGVCTLVLAQASLSLPFAWTQIRASFDRIPVAL
                     DEGAALLSTSSLDRQFRVRLPLVARGIQSAYALSFAIACGDATLPLVLSLDRFENLSL
                     LVYRLAGSYRFAEASAAAVFLALLSSLVFFLQDGQSKVEDAR"
BASE COUNT          494 a          516 c          454 g          334 t
ORIGIN      
        1 cgtactctcc aataaaaatc tttccctcaa gaaaggttcc cgtagtcagc acaacagtac
       61 gggcggatat acttcttcca cgagacgtgc gtaccgcggt gataacaccc tgatctacat
      121 atccactctc gtcggctccg gctatttcaa gggctaccac tgtatcctga tatatcgaaa
      181 gccccggttg catctccagc gtatgcttgg ctaatagatg atagcgatat ttgtccgtct
      241 gaacgcgagg cgcctgaacc gcaggacccc ggctcttgtt aagcaggcgg tactgtatca
      301 tcgtcgcgtc agcgagccgg gccatttctc ccccaagggc atcaatctcc cgtacgatat
      361 ttcccttgga aataccgcca attgaggggt tacaggataa ttttccgatt gaatcaagcg
      421 tttgggtaat cagtagggtg gaaaggccca tgcgggcggg ggcaagcgcg gcctcaatgc
      481 cggcatgacc gccgccaacc acgattacgt catagtcagt atagcgaaaa cccatagacc
      541 gggagtatac ccgcccaggc gggtatctgt ctattattcc attaccagca ctcgttcggc
      601 caatcgctgt gcttcctctc gatcgtgggt tacatagacg gcagtatacc caagctcacg
      661 ttgacgctcc cggagttcac ttcccaatcg cacccgcaac tcctgatcca aagcggagag
      721 cgcttcgtca aacagtacaa ccgcaggctc taccgcaagg gtgcgggcaa gggacaccct
      781 ctgtcgctcg ccaccagaaa gactgtcaac attccgccgg gcaaaaccgg aaagggaaaa
      841 tcgctcaagc caaaaagccg ctatctgtct gctttctttt tttgttttcc ccgcggagcg
      901 cggcccataa ccaatattgt cttccaccga taaatgaggg aacagggctg attcctgaaa
      961 gaccatacca acccctcttt tccccggcga aagagcggtt acatcccttc ccccaatcac
     1021 tacccttccc gaatctggtg gtaaaagacc ggcaataatc ctgagtacgc tggactttcc
     1081 gcaccccgag ggtccaagca gggcaagaga ttcacccgtg tgcaaggtaa aactcagaaa
     1141 gatatccttc atgggccagt gtttaacaac agagatcagt tccaggctca tcgagcatcc
     1201 tccactttag actgtccatc ctgcaagaaa aaaacgagcg aacttagtag tgcaagaaac
     1261 acagcagcgg cgctcgcttc ggcaaaccga tacgaacccg ccagacgata caccaagagc
     1321 gacaggtttt caaagcgatc gagcgaaaga accaggggca aggtcgcgtc tccgcaggcg
     1381 atcgcaaacg aaagggcata ggcagattga attccgcggg caactaaggg caagcgaacg
     1441 cgaaactgac gatcaagaga gctcgtcgac agaagtgcag ccccttcatc aagggcaaca
     1501 ggtatccggt caaaggaagc gcgtatctgc gtccatgcaa agggaagaga cagggatgcc
     1561 tgcgcaagga caagggtaca cacgcccata cgcggagcaa gaactatcca tccaaaacca
     1621 agcattaccg aagacaccgc caggggtacc aagggcaaaa cccgggtcac agctgaacgg
     1681 cttctcccca ctaaaagagt cagagccagg gctgcaatgg tagaaagaaa ggccgcgcag
     1741 gaggacaccc agacggttgt acccaacgag cgaaagaaag agggccggga aaaaaggg
//
LOCUS       DAOQXQ010000401         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79013_length_1793_cov_3.632336, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000401 DAOQXQ010000000
VERSION     DAOQXQ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79013_length_1793_cov_3.632336"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PKH81_06875"
     CDS             complement(<1..1605)
                     /locus_tag="PKH81_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ97796.1"
                     /translation="MNSRATPLLIKIGSGFCPDLRVMEFHINGETRKKCDFDQSLYQS
                     SGNVIFANIENVQQFAKKYNDLVDGKNHSERYLKTGQLNAMALIDEIFHYVCRLYRQD
                     ADKGFFVEGLSHLESSVGKAGVDRLLLAFTQQFPPVAVYQGHTSAELWLKDSDTDGTT
                     NRERALEELMMLTLANDNPAFAPFYPLFDNTALIQDPLWTPFWDSLRSWSAIKPAFGP
                     EGFDLISMLKAPVDYSPFDLKGQLDYIRTRWGTLLGDWLMRLLSSLDLIAEENKPGWA
                     GLGDGSIDMDPYDYTDISREYERFSPDRDWMPNVVLMAKSALVWLSQLSRTYGRAITR
                     LDQIPDEELDSLARAGFTGLWLIGLWERSPASARIKQICGNPEAAASAYSLDDYEIAR
                     ELGGWEALDNLRTRAWKRGIRMASDMVPNHTGMDSNWVVEKPDLFIQAPESPFPGYDF
                     SGEDLSHDGRVGIFLENHYYSKSDCAVVFKRVDKSTGDTRYIYHGNDGTGMPWNDTAQ
                     IDFLNPTAREEVIQKILHVARNFPIIR"
     gene            complement(1605..>1793)
                     /locus_tag="PKH81_06880"
     CDS             complement(1605..>1793)
                     /locus_tag="PKH81_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97797.1"
                     /translation="SDTTAQNPNDERINIPGTVSPFNWTWRLPLNLESLIKNHHLTQA
                     IAAICAAHQKESTTGGKS"
BASE COUNT          419 a          428 c          460 g          486 t
ORIGIN      
        1 acggatgatg gggaaattgc gcgcaacgtg caatatcttt tgtatgactt cttctctggc
       61 tgttggatta agaaaatcaa tctgtgcggt gtcgttccag ggcataccag ttccatcgtt
      121 accgtggtag atataccggg tgtctcctgt tgatttatca actcgtttaa aaacaacagc
      181 gcaatcgctt ttagagtaat agtgattttc cagaaaaatg cctacgcggc catcgtggga
      241 tagatcctct ccggaaaaat catagccggg gaaaggactc tccggcgctt gtatgaagag
      301 gtcaggcttc tccacaaccc aattggagtc catgcccgta tggtttggaa ccatgtcgct
      361 tgccatgcgg attccacgtt tccatgcccg tgtgcgcagg ttgtccaagg cttcccaacc
      421 gccaagttcg cgggcaatct catagtcgtc caaactgtac gcgcttgcag cagcttccgg
      481 attcccgcaa atctgcttta tccgggcgct tgcgggggat cgctcccata atccgattag
      541 ccacaggccg gtaaagcctg ctcgtgccag tgagtcaagt tcttcatcgg gaatctgatc
      601 caggcgagtt atagcccttc catatgttcg cgatagttgg ctaagccaga ccaaggcgct
      661 tttcgccatg agtacgacat tgggcatcca gtcgcggtcc ggactaaagc gttcatattc
      721 tcggcttatg tctgtatagt cgtaaggatc catgtcgatg gaaccgtcgc caagacctgc
      781 ccatcccggt ttgttttctt cggcgatcag atcaagagag gacaagaggc gcataagcca
      841 atcgcccaaa agggttcccc agcgtgttcg gatataatcg agttggccct taaggtcaaa
      901 gggtgagtag tcaacagggg ccttgagcat agatattaga tcaaaacctt ccggtccaaa
      961 agcaggtttg attgcggacc aggagcgaag cgaatcccaa aaaggtgtcc acaggggatc
     1021 ttgaatcagc gcggtattgt caaagagcgg ataaaaaggg gcaaaggccg gattgtcatt
     1081 ggcaagggtg agcatcataa gctcttcaag agcgcgttcg cggtttgtcg tcccgtctgt
     1141 atcactgtct ttgagccaga gctcggcaga ggtgtgtccc tggtataccg caaccggcgg
     1201 gaattgctgg gtaaaagcaa gtagaagtcg atctacgcca gcttttccaa ctgaggattc
     1261 aagatgcgat aagccctcta caaaaaaacc cttgtccgca tcttgtctgt atagacggca
     1321 tacatagtgg aatatctcgt ctatcagggc catggcgtta agctgccctg tcttaagata
     1381 tcgttcgcta tggttttttc catccaccaa gtcgttgtac ttttttgcaa actgttgaac
     1441 gttctctatg ttcgcaaaaa taacgtttcc actcgattga tatagcgact gatcaaagtc
     1501 acattttttt cttgtttctc cattgatatg aaattccata acgcgcaggt ccgggcagaa
     1561 cccagatcct atcttgatga gtagtggcgt ggcacggctg ttcattaact ctttcctccg
     1621 gttgtgcttt ctttctggtg tgctgcacag atggcggcga ttgcttgcgt aaggtgatga
     1681 ttttttatta ggctttctag gttgagcggg agcctccagg tccaattaaa ggggcttacc
     1741 gttccgggaa tattgattcg ctcatcattc gggttttgag cagttgtgtc gga
//
LOCUS       DAOQXQ010000402         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79046_length_1793_cov_2.283084, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000402 DAOQXQ010000000
VERSION     DAOQXQ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79046_length_1793_cov_2.283084"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..447
                     /locus_tag="PKH81_06885"
     CDS             172..447
                     /locus_tag="PKH81_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97798.1"
                     /translation="MATQDRYALIIDPYVSLRDEPGDSGITVSHGRRGDIVIITGRKI
                     VSSEGKRSKWIHSSKGWLPESSVYLYSSEQTARTAAQELLNGEHPKP"
     gene            complement(444..1493)
                     /gene="rlmN"
                     /locus_tag="PKH81_06890"
     CDS             complement(444..1493)
                     /gene="rlmN"
                     /locus_tag="PKH81_06890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNQ97799.1"
                     /translation="MNTEHPATSALSGMDSVEMAALLPLDKPFRAKQIYRWIANGARN
                     FEVMTNLSASLRDELSSRFTVFSTSISQTLTDSDGTVKLQIELSDGSMVETVLLTDEK
                     GRKTACVSCQVGCPMGCAFCQTGHIGYLRNLEAAEIIEQFYHLEDSVGKLDNIVFMGM
                     GEPLLNLRAIRKTIAILTDPEGRGLSKRRITISTSGLCEGIISLADEGPTVRLAVSLT
                     TANLELRKSLMPVTLSNPLPELKKAIAYFSEKTGSRVTLEAAVMAGINTDELSAQEMA
                     SFARDLNVHINIIPWNPVSGLPFTTPTNKEVRAFVHALVRLGLNATVRMRRGKSIQGA
                     CGQLGSRPTEERNDI"
     gene            complement(1503..>1793)
                     /locus_tag="PKH81_06895"
     CDS             complement(1503..>1793)
                     /locus_tag="PKH81_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ97800.1"
                     /translation="EEAQQYYQRALDIEFDVYAVLGLAMISKTQGKYDDAAVSLNRLI
                     QADPKNYRLYLELAECHVHKGDQQKAIQTLQEFQKLGIRNQLVSEMMDKLRG"
BASE COUNT          444 a          408 c          414 g          527 t
ORIGIN      
        1 ttgaaaatct ggaatttgtc gattcagatc ggcttctttt accggctctt cgggaatggg
       61 tgaaacatgc tcggtaagcg attatgtgcg tgtgtacttt gtgccttttg ttttgtatcc
      121 tgttcgcgta aaacctcgtt tgccgattta tcttttccat cctcgtctag ggtggctaca
      181 caggaccgtt acgccttgat aattgatccc tatgtatcgt tgcgcgatga gccgggagat
      241 tcaggcataa cagtctctca tggtcgacgg ggagacattg taataataac tggcagaaaa
      301 atcgtttcaa gcgaggggaa gcgaagtaag tggattcatt catctaaggg ctggcttcca
      361 gaatcctcgg tctatcttta ttcaagtgaa caaacggcac gtaccgctgc ccaagagctc
      421 cttaatggag aacatcccaa accttagata tcgtttcttt cctctgttgg ccttgatcct
      481 aattggccac aggcaccctg tattgattta cctcttctca ttctgactgt ggcattaagc
      541 ccaagacgta ccaaagcgtg tacaaaggct cgaacttctt tattcgtcgg ggtagtaaag
      601 gggagtccag agactggatt ccaggggatg atattgatat gaacgttcaa atcgcgggca
      661 aagctggcca tttcttgagc gctcagttca tcagtattta ttccggccat aacagcggct
      721 tcaagcgtta cacggcttcc tgttttctcg gaaaaatacg caatagcttt tttaagttcg
      781 ggtaagggat ttgatagagt taccggcata agcgattttc gtaattctaa atttgcggta
      841 gtcagggaca cagccagacg gactgtcggt ccttcatctg caagtgagat aattccttca
      901 cagaggccgg aggttgatat ggtaattctt cttttggaaa ggcctcttcc ttcgggatct
      961 gtcaaaatag caatggtttt acggattgcg cgaaggttca gaagcggttc tcccatcccc
     1021 ataaaaacga tgttatccag ttttccaaca gaatcttcca gatgatagaa ttgctcgatt
     1081 atttctgctg cttccagatt acgaagatac ccaatatgtc cggtttggca aaaggcacat
     1141 cccatcggac agcctacctg acaggataca catgctgtct ttctgccttt ttcatcggtt
     1201 aacagtactg tttcgaccat actgccatcg ctcagttcaa tctgtagttt aacggtacca
     1261 tcagagtcgg taagggtttg ggaaattgag gtagaaaaaa cggtaaagcg ggaagaaagt
     1321 tcgtcccgta aactcgcgct caggtttgtc atcacctcaa agttacgagc cccgttcgca
     1381 atccatcggt aaatctgttt tgccctaaac ggcttgtcca gtggaagtaa tgcggccatt
     1441 tctacgctat ccataccgga tagcgctgat gtggctggat gttctgtatt cataaaaaaa
     1501 ccttagccgc gaagcttgtc catcatctcc gagacaagct gattgcgtat gccgagtttc
     1561 tgaaattcct gaagggtttg aatagctttt tgctggtcgc ccttatgaac atggcattcg
     1621 gccaactcaa ggtatagtcg gtaattcttg ggatcggctt gaataagtcg attaaggctt
     1681 accgcagcgt catcatattt cccttgcgtc ttgcttatca tggcaaggcc taataccgca
     1741 tatacatcaa attctatatc caaagctcgc tgatagtact gctgggcttc ctc
//
LOCUS       DAOQXQ010000403         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79108_length_1792_cov_2.649971, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000403 DAOQXQ010000000
VERSION     DAOQXQ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79108_length_1792_cov_2.649971"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKH81_06900"
     CDS             complement(<1..647)
                     /locus_tag="PKH81_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97801.1"
                     /translation="MVTKEKIPVPPVCVAFPALAVFLYALLQFNWGESTIVLSMAALL
                     PLFFLVHSYLALHRLIVAPQQAPVIPRYLKVLFFSAIVSLALYSRIRIEEQTPKSLLP
                     IHSVQAIEAILLEDPVPAGNQWYRAHCELISFIDEAGSIYSARGSTCLLLPSTLIREN
                     LPYGLTGTNYSQSYYRGMTVRLCGTWKEQQLPSNVGLAFFAYEAVPENRSAFIIR"
     gene            complement(641..>1792)
                     /locus_tag="PKH81_06905"
     CDS             complement(641..>1792)
                     /locus_tag="PKH81_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97802.1"
                     /translation="ISLVTAFLVAQSPDPVVLSYQRNFIRASISTKIELLNDASRITT
                     VNMTPLYTDSISFVLTNYPVLGIDAQLLEIASVSAQKAAQYKDPSINESLKRLFTGVN
                     ETRVRVACIQALSSLAKGKQTDIAFLEKWFSDSIDASVSGKGGDVKVLAICADALGKL
                     SSPSSFPVLFRAATSSLDSSIVQASSVSLNSISEGYTDNILAIIRDKGIKEMYAAFSF
                     SAKKENLANTDKGKIAEAAFTRAITIQSSGTDAGLQASLITESMASLSNLKWSGASAA
                     VVRYFYRVQSEYKSDVSGIDQLIPVINCMGSMATTEAAQALSIYLGLLNSEAEQKKAY
                     NEQILLAVIRALGELGDKTAFDYLLYVGYLDYPESVKQASRDALARLQW"
BASE COUNT          532 a          389 c          412 g          459 t
ORIGIN      
        1 tgccggataa tgaaggcaga tcgattctct ggcactgcct catacgcaaa aaatgccagg
       61 ccaacattac tcggtaattg ctgttctttc catgtcccgc aaaggcgtac cgtcattccc
      121 cgataatatg attgactata gttggtgcca gttaagccat aggggagatt ttctcgaatc
      181 aaagttgaag ggagtaacaa gcaggttgat ccgcgagcgg agtatataga acctgcttca
      241 tcaataaaag aaatcagttc acagtgagca cgataccatt gatttcctgc gggtaccgga
      301 tcttctaata gaatcgcttc aatagcttgt acagaatgga ttggtaagag gcttttgggt
      361 gtttgctctt caatacgaat tcttgaatat aaggccaaag atacaatagc agaaaaaaaa
      421 aggactttga gataacgagg gattactggc gcctgttgtg gggctacaat aagtcgatgg
      481 agcgccagat aactgtgtac cagaaaaaaa agcggcaata gtgccgccat gcttagtaca
      541 atagtacttt ctccccaatt gaattgaagc agggcataca aaaatacagc caaagcaggg
      601 aatgctacac atactggagg aacaggtatc ttttcttttg ttaccattga agcctcgcga
      661 gcgcgtcgcg agaggcctgt ttgacagatt ccggataatc gagatagccc acatacaaga
      721 gataatcaaa agcggttttg tctccaagct ctcccaaagc gcggataaca gcgagcaaaa
      781 tttgctcatt ataagccttc ttctgctccg cctctgaatt taaaagtccc aggtaaatag
      841 acaaggcctg tgcagcttcg gtagtagcca ttgatcccat gcagttaata accggtatga
      901 gctgatcaat gcccgaaacg tcgctcttat actcactttg gacgcggtaa aaatagcgaa
      961 ccacagccgc gcttgctcct gaccacttca agttggacaa actcgccata gattccgtaa
     1021 taagagaggc ttgtaatccc gcatctgttc cactcgactg aatggtaatg gcccgagtaa
     1081 acgccgcttc tgcgatttta cccttgtcgg tatttgccag gttttccttt ttggcggaaa
     1141 aggagaaggc tgcatacatc tcttttatgc ccttatctcg tatgatggcc aagatattgt
     1201 cagtataccc ttcggaaatt gagttaaggg agaccgagga agcttggact atactgctgt
     1261 ccaaactgga agtagcagca cgaaagagta cgggaaaaga agagggagag gaaagctttc
     1321 caagggcatc ggcacatata gccaatactt ttacatcgcc acctttcccg gatacacttg
     1381 catcgatgga atcagaaaac catttttcca aaaaagcgat atcagtttgc ttgccctttg
     1441 ccaaagaaga aagagcctgg atacaggcca ctcgaacacg agtttcgttg acgccggtaa
     1501 agaggcgttt aagtgattcg tttatcgatg gatccttata ctgtgctgcc ttttgagcag
     1561 ataccgaagc tatttccaat aattgtgcat cgattcccaa aacaggataa ttggtcaaca
     1621 caaaagagat tgaatcagta tacaggggtg tcatattaac tgtagtaatt cgagatgcat
     1681 cattgagtag ctcaatcttt gtactgatgc tcgccctgat aaagtttctt tgataggaca
     1741 atacaacagg atccggtgat tgggccacca aaaaggcggt tacaaggctt at
//
LOCUS       DAOQXQ010000404         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79185_length_1791_cov_2.568548, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000404 DAOQXQ010000000
VERSION     DAOQXQ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79185_length_1791_cov_2.568548"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKH81_06910"
     CDS             complement(<1..717)
                     /locus_tag="PKH81_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ97803.1"
                     /translation="MIEATERVSENRKVRYPLVVKLIGIISLIVVVSMLAITALATWF
                     FAEDSRSRSEDSSLTLNQMTAMQMESEVRALHSGALSLMDTLREAGTNRLLAAASITN
                     WFERNAVVAALIVPQEHTIYNDKFFLANELEKETVQSFLVARSSDIERSRAGEYLLVN
                     ASPFLGIPAAALLVPYRDLGTENLLVIVFSTENLQNIVRSDTLGRTYAVSQDGELIAH
                     SDFSLVKLGVNMKGDPLVKQT"
     gene            complement(742..>1791)
                     /locus_tag="PKH81_06915"
     CDS             complement(742..>1791)
                     /locus_tag="PKH81_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97804.1"
                     /translation="GSSFDKANPYAGGWRYLEIMNLVTNSTASWGRSGVYYGATRSGI
                     GEGRINMAILKDHTDAVSAVGRCRAYTGGGFSDWYLPTTGELNDAYSKSALLSGGHWS
                     SEEQNATTIYNSANGTTNTYTKTGTNNVSAVRSFAGSEPTKMVAYVTVPAEIQSTNGG
                     SVPSSPQLYPVGQSVPVLGNIGGLTHIADFSFMGWSTRPGGRGTTYLPGTTLPVDSGD
                     VVLYPRWQVPGYDTLDFAKVTALTGAATAAPVDNSDANRLMVNDILVYKLNNGNVGKI
                     RIDNADYGSNHGLQFYWQEWTSGGVDVGNNSAVIEGTAYWNRDGTNVFHLQNFTGTER
                     AIVMENGSIIYYLGQ"
BASE COUNT          406 a          470 c          446 g          469 t
ORIGIN      
        1 ggtttgtttg accagtgggt ctcccttcat attcacgccg agttttacca gagagaagtc
       61 gctatgggcg atgagttctc catcctgcga tacggcatag gttcggccca gggtgtccga
      121 tcgtacgatg ttttgtaagt tttcggtaga aaaaacgatg acgagaaggt tctccgttcc
      181 caggtcgcga tacggtacga gtaaggctgc ggctgggatg ccaagaaagg ggcttgcgtt
      241 taccagaagg tattctccgg cgcgcgagcg ttcgatgtct gatgagcggg cgacaaggaa
      301 gctctgtacg gtttcttttt ccagttcgtt cgccaaaaag aatttgtcgt tatatatggt
      361 gtgctcttgg ggtacgatca gggcggcgac tacggcgttt cgctcaaacc agtttgttat
      421 gcttgcggcg gccagtagcc ggtttgtccc cgcttcccgc agggtatcca tcaggctgag
      481 cgcgccggaa tggagggcgc gaacttcgct ttccatctgc atggccgtca tttgatttaa
      541 ggtaaggctg ctgtcttccg agcgcgacct tgagtcttcg gcaaagaacc aggttgccag
      601 ggcggttatg gcgagcatcg aaacaactac aataagagat atgatgccaa tgagttttac
      661 gacgagtggg taccgtacct tgcgattctc cgatacccgc tctgttgcct ctatcatgtc
      721 tgtatttctc cccattatgt attattgccc cagataatat atgatcgagc cattttccat
      781 aacaatcgcc cgctcggttc cggtaaagtt ttgtaagtgg aatacattcg tgccatcacg
      841 attccaataa gcagtgcctt cgattacagc cgaattatta ccaacatcaa caccaccaga
      901 agtccattcc tgccagtaaa attgaagccc atgattactg ccatagtccg cattatcaat
      961 cctgatcttc cctacgtttc cattattcag cttgtacact agtatatcgt taaccataag
     1021 gcggtttgca tccgagttgt ccaccggagc tgcggttgcc gcaccggtga gcgcggttac
     1081 ctttgcaaag tccaaagtat catagcctgg aacctgccag cgggggtaaa gtacgacgtc
     1141 gcctgaatca actggtaaag ttgttccggg aagataggtt gttccccttc cgcccgggcg
     1201 ggtactccac cccataaaag agaagtccgc aatgtgggtc agcccgccga tgttccctaa
     1261 tacgggaacg ctttgaccca ccggatagag ctgcggcgac gaaggaacgc ttcctccatt
     1321 agtgctttgt atttccgccg gcaccgttac gtaggcgacc atcttggtag gctccgaccc
     1381 cgcaaaggag cgcacggcgg agacattgtt ggtgcctgtc tttgtataag tatttgttgt
     1441 gccatttgca gaattataaa tagttgtcgc attttgctct tcagaagacc aatgaccccc
     1501 actcagcaat gcagacttac tatacgcatc gtttagctcc cctgttgtgg gaagatacca
     1561 atcgctaaaa ccaccgcctg tataagcgcg gcatcggcct accgcactta cagcatcggt
     1621 gtgatctttc aggatcgcca tattaatgcg tccctcgcca ataccgctcc gggtagcccc
     1681 ataatagact ccgcttcttc cccaactggc agtagaattc gtaacaagat tcattatttc
     1741 caaataccgc cagccgcctg cgtaggggtt tgccttgtca aaacttgagc c
//
LOCUS       DAOQXQ010000405         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79231_length_1790_cov_3.539481, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000405 DAOQXQ010000000
VERSION     DAOQXQ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79231_length_1790_cov_3.539481"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKH81_06920"
     CDS             <1..669
                     /locus_tag="PKH81_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNQ97805.1"
                     /translation="FVLIYWERRGEWIVGCELDMDAVREKISELAGNPSDAVRSLAIL
                     DQAGLPLVLVPGVPLESWRTPLVSGEISEALPYWETAVILTDASAFESRVRSSRYALT
                     ALVLSLAMSVSLGAFFLWRYSANQLLEARKRTGFVTTVSHELKTPLTSIRMYSEMLAE
                     GADDDHEKRKRYLDRIVGESERLTRLINDVLDVAKLRQKIEHDTANPLHILTVHGVGY
                     RFRL"
     gene            714..>1790
                     /locus_tag="PKH81_06925"
     CDS             714..>1790
                     /locus_tag="PKH81_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HNQ97806.1"
                     /translation="MDNLGAALANGLSYFLTFKPYVMLPVIIFIVSLVFGIKWSSAVK
                     SALQLGIAFIGIFMVFDYFVALIKPVTEALIARSGLAMNVLDVGWPPLAAITWSFSLA
                     PVLLIAFLVINMLMLYFRLTRTVNIDIWNFWHVIFVASLVYFITDNYWLAASVSLACF
                     VLTLKLAEWSAPMITTYCGMDSVSVPHLSAITHFPLARLGVSVLSRIPFIRSVRADPE
                     HIKTKLGILGEPMILGFFLGAALAFGGGYTVKGILEMAFGFAAVILILPIMGGVLGSA
                     LLPVSEGMKEFLEKRLPGRGTTYIGLDVAILFGVPATIVSAVIMIPISIGLAFVLPGV
                     RFIPLGDLTNLMVPMSIICAAAKGN"
BASE COUNT          380 a          390 c          461 g          559 t
ORIGIN      
        1 tttgtgctga tttattggga acggcgtgga gaatggattg tcggttgtga gctcgacatg
       61 gatgcggttc gggagaaaat ctcggaactc gccggaaatc cttccgatgc cgtgaggagt
      121 cttgccattc ttgatcaggc aggtcttcct cttgtactgg tccccggagt accgcttgaa
      181 agctggagaa cgcctcttgt atcaggtgaa atcagcgaag ccctgcctta ctgggaaaca
      241 gctgttatac ttaccgatgc atcagccttt gaaagccgtg tgcggtcatc ccgttatgcc
      301 cttacagcgc tcgttctttc gcttgctatg tcagtatcgc ttggtgcttt ttttttgtgg
      361 cgctattccg cgaatcagct tttggaagca aggaagcgga caggatttgt tacaaccgtg
      421 tcgcatgagc taaagactcc tcttacctct atcagaatgt attcagagat gctggcagag
      481 ggagctgatg atgatcatga aaaaaggaaa cgttatctgg acaggatcgt aggtgaaagc
      541 gagcgcttga caaggcttat taacgatgtc cttgatgttg caaaactcag gcagaaaatc
      601 gagcatgata cggcgaatcc ccttcacatt ttgactgttc acggggttgg gtatcgattc
      661 aggttgtgac ttaaactata atagaaagcg tttactcgta agtggaggaa tagatggata
      721 accttggtgc agcgttggca aatggacttt cgtatttttt gacctttaag ccctatgtca
      781 tgcttccggt aataatcttt attgtgtcgc ttgtgtttgg tattaaatgg agctccgctg
      841 tcaaatcggc tctgcaactc ggcatagcct ttattggtat ctttatggtg ttcgattatt
      901 ttgtagcctt aattaaaccg gtaaccgaag ccctgattgc ccgttcggga ttggcaatga
      961 atgttctaga tgttggttgg ccgccattag cagcaattac ctggagtttc tccctggctc
     1021 ctgttttgct tattgcgttt ctggttatta acatgcttat gctgtatttc cgcctaacgc
     1081 ggacagtaaa tattgatata tggaactttt ggcacgtgat cttcgttgca tctcttgtct
     1141 attttattac tgataattat tggttggctg cctctgtatc gcttgcctgc tttgtgttaa
     1201 cccttaaact tgctgagtgg agcgcgccaa tgataaccac gtactgcgga atggactcgg
     1261 tgtcggtccc gcatctatcg gctattacgc actttcctct tgctcgcctt ggggtgtccg
     1321 ttctttcccg aatacctttt atccggtcag ttcgggctga ccctgaacac attaagacaa
     1381 agcttggcat ccttggtgag ccaatgattt tgggcttctt tttgggggcc gcccttgcct
     1441 ttggcggcgg gtataccgtt aaggggatac ttgagatggc cttcgggttc gccgctgtta
     1501 ttcttatcct tccgataatg ggcggggtat taggctccgc cttgctacct gtcagtgagg
     1561 gtatgaaaga gtttttagag aagcgacttc cgggaagggg aacgacatat attggtcttg
     1621 atgttgcaat cctttttgga gttccggcaa caattgtaag cgcggtaatc atgattccaa
     1681 tatccattgg acttgccttc gttcttccag gggtgcgctt tatcccgctt ggggatttga
     1741 cgaacctgat ggtgccgatg tccatcatct gcgccgcagc aaaaggtaac
//
LOCUS       DAOQXQ010000406         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79297_length_1789_cov_3.769319, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000406 DAOQXQ010000000
VERSION     DAOQXQ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79297_length_1789_cov_3.769319"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..725
                     /locus_tag="PKH81_06930"
     CDS             465..725
                     /locus_tag="PKH81_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97807.1"
                     /translation="MRLPKLKRGDAILVEWIDAHFLSDAGWRDEAEYVELEPITITTV
                     CQFIMRDRDYIYTVADRSKTEPIGIMRDLRIPIGCLRRVTKL"
     gene            1038..1316
                     /locus_tag="PKH81_06935"
     CDS             1038..1316
                     /locus_tag="PKH81_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97808.1"
                     /translation="MTNLTKAQFAVIEQELCRRVNLPYPPPNGPYEVTKDATWTEAEE
                     AEFKKWLVKYLQKLPELKRMRKASIENWAGWIVFDLGWKISERESCQK"
     gene            1394..1630
                     /locus_tag="PKH81_06940"
     CDS             1394..1630
                     /locus_tag="PKH81_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97809.1"
                     /translation="MDTKTNRDVWVTTTEEFILNYAALPKEFEDWRYYRIEYYGDGPY
                     AIKECSFFAPPWFDRDTFEKLFERPKKWNEIPNR"
BASE COUNT          529 a          375 c          466 g          419 t
ORIGIN      
        1 gggccgggac aaagttttca gcatttaaga ggcttatcaa aaagggcggc ggaaaagtct
       61 atgtggagga gccatgataa ccgtatctat cttcatcaat gacacaccca tttacacccg
      121 ttcggccaga aacgtaacag tggatttcgg ccagaaaata cacagctata aattggacac
      181 gggcgaaacg attaagtatg agcgggcttc gggggccgtg cctctcgcta tcaagatgct
      241 caaaaccata aaggagccgt gatgatttac atagagacaa cttatggttt tatctacggt
      301 tcggcaaacg tagaacgtcg tatgtccgac gagaaaaagg gctgggtagt tattgggatc
      361 aagaccccca aagccgacat acaagtatat gtcaccaaga cggggaaagt gagaatctat
      421 aactattatg gtagggaatt tatcgaaaaa cctaaggagc cgtgatgcgt ctgccaaaac
      481 tcaaacgtgg tgacgctatc ctcgtagagt ggatcgacgc gcatttcctg tcagatgcgg
      541 gatggcgcga cgaggcggaa tatgtggagc ttgaacccat aacgattaca accgtctgcc
      601 aattcatcat gcgagacagg gattacatct ataccgtggc agacagaagc aagacagaac
      661 caatcggcat catgcgggat ttgcgaatac cgattggatg tttgcggagg gttacaaagc
      721 tatgaatcta acaggcaaat acgtctatct ggccggaccg tatagcatcg gagatcaggc
      781 cgaaaatgtc cgcaatatga ttcattacgc agacaggctt atggacatgg gcgcaatcgt
      841 ctataacccg ttgttgtcgc attttcacca aatctattcg ccgcggtcga aatccttttg
      901 gtacgagcaa acactaaaat ttgtcgcaaa gtgcgacttg ttgatttttc tgccgggaga
      961 gagccgggga gctgagcggg agattcagca cgcaaaggat ctatggattc ctgtgtatcg
     1021 gattgaggag gtatgttgtg actaacctta caaaagcgca atttgccgtg atcgagcagg
     1081 agctttgccg ccgcgtaaat cttccatatc cgccgcccaa tggtccatac gaagttacta
     1141 aagatgcgac atggaccgaa gcggaagagg cggagtttaa aaaatggctc gtaaagtatc
     1201 ttcaaaagct accagagctg aaacggatgc gaaaggcatc catcgagaat tgggcgggct
     1261 ggatcgtgtt tgatttgggc tggaaaattt cagagcggga atcttgtcag aaataatgct
     1321 cattttttct gataaaatat caggacggga atagtttggt tttatatgca ttacgtgtac
     1381 ctgggggaca caaatggaca caaaaacaaa ccgcgatgtg tgggtgacga caacggaaga
     1441 atttatcctt aactacgccg cacttccgaa agagttcgag gattggcgat actatagaat
     1501 cgagtattac ggcgacggtc cttatgcaat caaagaatgc tcattttttg cgccgccttg
     1561 gttcgatcgg gacacgttcg agaaactttt tgaaaggccg aaaaaatgga acgagatacc
     1621 caatcggtga atgcaccgaa atgcccaatc tgtcaaggca caggctttta ccgtggtcaa
     1681 atctgcacct gcatcaaggg taaagattcc gacatcccga aggaattgag ggatattttc
     1741 ggggatgttt tcggtgacat attcggggac aaaaagaccc acacttaat
//
LOCUS       DAOQXQ010000407         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_79527_length_1786_cov_3.347198, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000407 DAOQXQ010000000
VERSION     DAOQXQ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79527_length_1786_cov_3.347198"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKH81_06945"
     CDS             <1..906
                     /locus_tag="PKH81_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNQ97810.1"
                     /translation="IGSVIIVIGLNLAPTAMGMAMNDPDGVYSLVRLSIAAFTLLVAI
                     IATIFFKGFFNVIPILIGLVSGYLFTLIMGMFFPAYKLISFDVVAKAPWFSLPTFMAP
                     KFNLMAALTFIVVSLATICEHLGDVMVTSRVVGEDFYKKPGLHRTLLGDGIATAWAAF
                     WGGPPNTTYGENIGVMAITRVYSVWVIGGAAVIAVILSLITKVGAIIQTIPTPVMGGI
                     SMLLFGIIASSGLRTIVESGVDFKCKRNLTISSVIMVIGIGGGTLAFNIGKDLKFQLA
                     GVALATVVGITLNLVLPKTIDTVKE"
     gene            999..>1786
                     /locus_tag="PKH81_06950"
     CDS             999..>1786
                     /locus_tag="PKH81_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNQ97811.1"
                     /translation="MNRVRCFSLFTLVIVLSSLPLSATVAFDSIWAYLMKGEERFFDT
                     SYPVSDIGYFGATMNSFGKLVDVPDPAALASFPGKKHLVVAEVTNRALTHFCLNPAYP
                     VREQLLGDIVLASKGYDGVQIDFELVSREDAAHFVSFLVDLKERIGKDKVLSIAVPAR
                     IKAIKDDAYDYKKLARVADTIIVMAYDEHWSGSAAGPVASMDWCRRVATYSKKAIGTS
                     KLVMGMPFYGRSWTDNRLAKAYKFSTLDTLLKEKAIQDIQRRQGV"
BASE COUNT          415 a          407 c          456 g          508 t
ORIGIN      
        1 atcggatcgg ttattatcgt tatcggtctt aacctggctc ctaccgcaat gggtatggca
       61 atgaacgacc cggacggagt gtatagtctg gtgcgtcttt cgatcgccgc ctttaccctg
      121 ttggtcgcga ttattgcgac gatctttttt aagggatttt ttaatgtaat tccgattttg
      181 atcggtcttg tctccgggta tctttttacc ttgattatgg ggatgttttt tcctgcatac
      241 aaactcatca gttttgatgt tgttgcaaag gccccctggt ttagcctgcc aacctttatg
      301 gctcccaagt ttaatcttat ggctgccttg acctttattg tggtatccct tgccaccatt
      361 tgcgaacacc ttggcgatgt tatggtaacg agccgagttg ttggcgagga tttttacaag
      421 aaacccggac tgcatcgcac cttgctcgga gatggtattg ccactgcctg ggccgcattt
      481 tggggcgggc cgcccaatac cacctacgga gagaatatcg gggtgatggc tattacccgg
      541 gtgtattcag tttgggttat cggcggagct gccgttatcg ccgtcatcct ttcgcttatc
      601 accaaggttg gcgccatcat tcaaacaata ccaaccccgg taatgggtgg aatctccatg
      661 cttctgttcg gaataatcgc ctccagcggt ttacgcacca tcgtggaaag cggtgtagac
      721 tttaagtgca aacgtaattt gacaatcagt tcggttatta tggtaattgg tataggaggc
      781 ggaacccttg ccttcaatat aggaaaagat cttaagttcc agcttgccgg agtggccctg
      841 gcgaccgttg tcgggataac cctcaatttg gttctgccca agaccatcga tactgttaaa
      901 gaataaataa caaactggta acgcggggat agcaataccc ccgcgtattc atcgatcccc
      961 ggctctatta gttggccaac acaccccgag gagttgtcat gaaccgcgtc agatgctttt
     1021 cccttttcac cttagtgatt gtactttcgt cccttcctct cagtgccacg gtcgcctttg
     1081 attcaatttg ggcgtacttg atgaagggag aagagcgatt ttttgataca tcgtatccgg
     1141 taagcgacat tggctatttt ggggcaacga tgaacagttt tggtaagctc gtggatgtgc
     1201 ccgatcctgc ggccctggcc tcctttcccg gaaaaaagca tctggttgtg gccgaagtga
     1261 ccaatcgggc gctcacgcat ttttgcctga atccggcata tccggttcgt gagcaattgc
     1321 ttggtgatat cgtgttggca agtaagggct acgatggtgt acagatagat tttgaacttg
     1381 tctcccgtga ggacgcagca cattttgtaa gttttcttgt ggacctaaaa gaaagaatag
     1441 gaaaggacaa ggtattaagc attgccgttc ctgccaggat taaggcgatt aaagatgatg
     1501 cgtatgatta taaaaaattg gcccgcgttg ccgacacaat tattgtgatg gcctatgacg
     1561 aacattggag cgggagcgcc gccggacctg ttgcgtccat ggactggtgc cggagagtag
     1621 cgacctattc aaaaaaagcg attggaacaa gcaaattagt aatgggcatg cctttttatg
     1681 gcaggtcctg gacagataat cgcctggcaa aagcctacaa gttttctacc ctggatactc
     1741 ttttgaaaga gaaagcgata caggatatac agcgccgtca gggagt
//
LOCUS       DAOQXQ010000408         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_80137_length_1778_cov_3.145096, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000408 DAOQXQ010000000
VERSION     DAOQXQ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80137_length_1778_cov_3.145096"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1037
                     /locus_tag="PKH81_06955"
     CDS             72..1037
                     /locus_tag="PKH81_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ97812.1"
                     /translation="MRIHFWGVRGSIPTPLSPLQIQNRIAAVVQRISARDIESADTRE
                     KFLASLPQWLFGTVGGNTSCVEVETSSGETIILDAGSGIRELSLNLRQRPSHAAGGNT
                     YHILFSHFHWDHLQGLPFFSQGFDPRDRIIFYSVRHDLPEILGQQMSSPYFPVPMQGP
                     GGFRSALEFVHLQSPDCQFKIGSSLVRWHEVNHPGGCVAYMIQEGGKKFIYATDTELT
                     RENFIRTSENTAFFADADVLVMDSQYTMGEALEKTGWGHSAFSMTVDFALNWNVKKLV
                     LFHHEPTYTDQKIFTLKQSADWYREYAGPGDLEIMIAREDQEIHL"
     gene            1034..1213
                     /locus_tag="PKH81_06960"
     CDS             1034..1213
                     /locus_tag="PKH81_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97813.1"
                     /translation="MIEEGHYTYTFSEAELRDLALLLRRMGRTLPRNLEDLSGFLESA
                     LYGIMTIEEAELFFQ"
     gene            1210..>1778
                     /locus_tag="PKH81_06965"
     CDS             1210..>1778
                     /locus_tag="PKH81_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNQ97814.1"
                     /translation="MKHNIVMRLIIFAVVICVSLFVGFVAFVHWADSPRSIEGEGALF
                     SIAKGASLVSIANSLHEQGFTRSPLYTTLYARYASYSLKAGTYRLSPSYKTSEILCRL
                     HEGKEESVRITIPEGLTVSKTARLLETEGIVSADAFIKAATKTALLAKFSIPASTAEG
                     FLFPDTYSLHYHMDAEQLVSLMISNFFTKT"
BASE COUNT          455 a          386 c          402 g          535 t
ORIGIN      
        1 gctggaaaaa aggcgtaagg agccgtatat tcatcgcaag gtaaccgaag tttagatcaa
       61 ggggtatcag catgaggatt catttttggg gtgtacgagg ttcaattcct acccccttgt
      121 ctccgctcca gattcaaaac agaatagctg ccgtagtaca aagaatttcg gcgcgcgata
      181 ttgaatcagc tgatacacgt gaaaagttct tagcatcact tccccagtgg ctttttggaa
      241 cagttggcgg gaatacttcc tgtgttgagg ttgaaacctc ttcaggagaa acgattatcc
      301 ttgatgctgg aagtggaatt cgggaactct ctcttaatct tcgccagcga ccttctcatg
      361 cagccggcgg gaacacatac cacattcttt tttctcattt tcattgggac caccttcaag
      421 gtctcccttt tttttcgcag ggtttcgatc cccgtgatcg catcattttt tacagcgtta
      481 gacatgactt acccgaaatt ttgggacaac agatgagttc tccttatttc cccgttccca
      541 tgcaaggacc aggaggattt cgctcggccc ttgagtttgt tcatttgcaa agtcccgact
      601 gtcaattcaa aatcggttcc agccttgtca gatggcatga ggttaaccat ccaggcggtt
      661 gtgtcgcgta tatgattcag gaaggcggaa aaaaatttat ttacgccacg gatactgagt
      721 tgactcgcga gaattttatc agaacgagcg agaataccgc gttctttgcc gacgctgatg
      781 ttttggttat ggattcgcag tacacaatgg gcgaagccct ggaaaagaca gggtgggggc
      841 attctgcctt ttctatgacc gtagactttg ccttgaattg gaacgtcaag aagctcgttt
      901 tgtttcatca tgagccgacg tacacagatc aaaaaatttt tactttaaag caatcagccg
      961 actggtatcg tgaatacgca ggtcccgggg atcttgaaat aatgatcgcc cgggaagatc
     1021 aggaaattca cttatgatag aagaaggcca ctatacctat accttttccg aagcggagtt
     1081 acgagactta gcacttctat tacgaagaat gggtagaacg ctacctcgta accttgagga
     1141 tttgtccggt tttttagaga gtgccttgta tggcattatg acaattgaag aggcggaact
     1201 tttttttcaa tgaaacataa tatagtcatg cgacttatca tttttgctgt agttatatgc
     1261 gtaagtcttt ttgtaggatt tgttgccttt gttcattggg cagactcacc tcgatccatt
     1321 gagggagagg gcgctctttt ttccattgcc aaaggcgctt ctctggtttc aattgcgaat
     1381 tcacttcatg aacagggttt tactcgctct ccgttgtata caacactgta tgcccggtat
     1441 gcatcgtatt cattaaaggc tggaacatat cggctttcgc cttcttataa aacctcggaa
     1501 attctttgtc gtttacacga gggtaaggag gaatccgttc gcattactat tccagaaggc
     1561 ttaacggtaa gtaaaacagc tcgtctgctt gagacagaag gaatcgtctc tgccgatgcc
     1621 tttatcaagg cggcaacaaa aacagcgctt ttggccaagt tttcaatacc tgcttctact
     1681 gcggaaggat ttctttttcc agatacctat agtcttcatt atcatatgga tgccgaacag
     1741 cttgtgtcgc ttatgatttc aaatttcttt acaaaaac
//
LOCUS       DAOQXQ010000409         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_80869_length_1768_cov_2.483363, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000409 DAOQXQ010000000
VERSION     DAOQXQ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80869_length_1768_cov_2.483363"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PKH81_06970"
     CDS             <1..1510
                     /locus_tag="PKH81_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNQ97815.1"
                     /translation="SEQDRVSIRKLSTGVPGLDEILGGGLPEFSFNIIAGGPGTGKTT
                     LAHQIVFANATPETPALYFTVLGEPPLKMMRYQQLYTFFDESKINSCIRFMNLSEIVK
                     EKNLHAVLEEITKQVEAASPGIVVVDSFQTVVRRPQLGTSEMELQAFVQDLALNLSGW
                     QATTFLLGEYNDRELLGNPVFTVADGILWLYQDSERNSVVRKLQVRKLRGQAAVPGMH
                     IFRITSSGLLTFSRFLGLSEEKQPTPSTKRLSVGIPELDAMMSGGVPEGDSILVSGGT
                     GTGKSLLAMQFLAEGIRQSEPGAALVFEEYSQGYMQRAKNFNLDLQTAQTNGNFSLIQ
                     LRAMDLCPDEILYEVLKVVDKIGAKRVVIDSLSGMELALAPEFRADFRESIYRIVRAL
                     TAAGATVLSTVEADESAICFPLSSYSASFLADNIIRLSYIELQGQLCKILFVVKMRGS
                     SHSKDIRDYEITSRGMVIGQNRFAGYRGLITGVPELMNEPNQKRGQESGGDL"
     gene            complement(1558..>1768)
                     /locus_tag="PKH81_06975"
     CDS             complement(1558..>1768)
                     /locus_tag="PKH81_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97816.1"
                     /translation="LSSFLGNTRFAPSGSADGLDTLKSTCFRDNLVKRQAHYVFSFFP
                     YQAGKGLVYASDNMPVTLDRCRRQV"
BASE COUNT          427 a          431 c          503 g          407 t
ORIGIN      
        1 gagcgaacaa gacagggtaa gtattcgaaa actttctacc ggcgttccag gcctcgacga
       61 gatcctgggc gggggtctcc cggagttctc ctttaacatt attgcagggg gtccaggaac
      121 agggaaaaca acgcttgctc accaaattgt cttcgcgaat gctaccccgg agacacctgc
      181 gctctatttc accgtcctcg gcgaacctcc cctgaaaatg atgcggtatc aacagctgta
      241 tacgttcttc gatgagagca aaataaacag ttgcattcga ttcatgaatc tcagcgaaat
      301 tgtgaaggaa aagaatctgc atgcagtgtt ggaagaaatc accaagcagg tagaggcggc
      361 aagtcccggt atcgtagtag tggattcctt ccaaacggtc gtgcgaaggc ctcaactcgg
      421 cacgagtgaa atggagcttc aagctttcgt gcaggacctg gccttgaatt tgagcggttg
      481 gcaggctacc acgtttctac tcggggagta caatgatcgg gaactgctcg gtaaccccgt
      541 tttcacggtg gccgacggca ttttatggtt gtaccaggat tcggaacgga attccgttgt
      601 aagaaaattg caggtaagga agcttcgcgg gcaggcagcc gtaccgggca tgcacatctt
      661 ccgcatcacc agctctggct tgctcacctt ttcacgcttc ctcggtcttt ctgaggagaa
      721 gcagccaact ccttcaacaa agcgcctttc cgtggggatt cccgagttgg acgcgatgat
      781 gagtggtggg gtgcccgaag gcgacagtat ccttgtatcg ggtggtacgg gaacggggaa
      841 atccctttta gcgatgcagt tccttgcgga agggattcgc cagagtgagc cgggtgccgc
      901 actcgtcttt gaagaatact cacaaggcta tatgcagcga gccaagaact ttaatcttga
      961 tctgcaaacc gcgcagacaa atggaaactt ctccctgatc caactgcgag caatggacct
     1021 ctgccctgat gaaatcctct acgaggtgct taaagtggtt gataagattg gggctaagcg
     1081 tgtcgttatc gattctcttt ccggcatgga actggccctc gccccggagt ttagagccga
     1141 ctttcgagaa tctatttata ggatagtaag ggctttgacg gccgcggggg ctaccgtcct
     1201 gagcacggtc gaggctgacg aatccgctat ctgcttcccc ttgagcagct actcggcttc
     1261 gtttcttgcc gataacatca tccggctcag ctacattgag ttgcagggcc agctctgcaa
     1321 gatccttttt gtagtaaaga tgcgaggaag cagccacagt aaagacatcc gagattatga
     1381 aattacttcg agaggtatgg taatagggca gaatcgcttc gcaggctatc gtggccttat
     1441 tacaggcgtt cctgaactga tgaatgaacc gaaccagaag cgagggcaag aatcgggggg
     1501 tgacctatag gcgcagaaac cgacctgcga aacctctgcg gtccggtggt ggagtattca
     1561 cacctggcgg cggcagcggt cgagggtgac aggcatatta tccgatgcgt aaaccaagcc
     1621 ttttccggct tgatagggaa aaaagctgaa gacgtagtgg gcttgccttt tgaccaggtt
     1681 gtcgcggaag caagtgctct tcaatgtatc gaggccgtct gccgaaccgg acggggcaaa
     1741 acgtgtgttg ccgaggaagg aactgagg
//
LOCUS       DAOQXQ010000410         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_81206_length_1764_cov_2.114102, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000410 DAOQXQ010000000
VERSION     DAOQXQ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81206_length_1764_cov_2.114102"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..564
                     /locus_tag="PKH81_06980"
     CDS             238..564
                     /locus_tag="PKH81_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97817.1"
                     /translation="MYSGGTITSSTTEDLTEIINAYNKEQAGAGDLFWGLMSMMAGTD
                     LDPATGEKTVITLTQNPPPYEMTIVSTMPDTEVTAEQLAKIEINEKGDKIRYPYEGSA
                     FVMEKQ"
     gene            661..>1764
                     /locus_tag="PKH81_06985"
     CDS             661..>1764
                     /locus_tag="PKH81_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNQ97818.1"
                     /translation="MAIQGQGAGLRPVVRVIEENCVNCHRCIAVCPVKMANDGSGDHV
                     RINGDLCIGCGECIDACTHGAREGIDDAEAFFGALARREKIVAIVAPAVVSSFPDDYL
                     RLNSFLAQMGVSAFFDVSFGAELTVKSYLEYKKEHNPKTIIAQPCPTLVTFIELYRPE
                     LIPYLAPADSPMLHTIKMIREYYPLYSAYKVAVLSPCFSKRREFDETGIGDYNVTFRS
                     LLDYIRKKGVVLSEYPEKEYDNPPAERAVLFSTPGGLMRTIERSVAGASEITRKIEGH
                     PQVFHFLAHMSEAIKTGAAPLHQVVDCLNCELGCNGGPGTNNRGKHADVIEQPIEQRN
                     RDMQKRYRPKGLFKFLGSNRKKLDALINQYWKKG"
BASE COUNT          464 a          390 c          446 g          464 t
ORIGIN      
        1 cttgaagaaa accagagtta gcgtatactg attccataca agattgtggc actgtgtgcc
       61 gtttttattg ctgctttatg tgttttctct ggctgtgatg aggcagaaag cgatacctgg
      121 accccggtaa cggataagtc tcaacttcag tgaagctgga agggagatag gcaaaaagta
      181 agcgttaagg acaagaatac cggggagagc tatgatgcga ccatttcagg ctctttggtg
      241 tattccggcg ggacgataac ctcttccaca accgaggatc taaccgagat tatcaacgcc
      301 tataataagg aacaagcggg agcgggggat ctattctggg gtcttatgag tatgatggcc
      361 ggcacagatc tggatcccgc tactggggag aaaactgtca ttacactcac ccaaaatcca
      421 cccccctatg aaatgactat cgtttctacc atgcccgaca cggaggtaac ggcggaacag
      481 cttgccaaga tagagattaa cgaaaaaggt gataaaatcc gctatcccta tgaaggttct
      541 gcctttgtga tggaaaaaca gtaataccct ttttgtttgc cagaattccc gacccatgtt
      601 actatgtaac cgcgggcgct gttgaagccc tgcttaaaaa gtgtgcaaaa gaggcagcaa
      661 gtggctattc agggacaggg tgcgggatta cggccggtag tgagggttat tgaggaaaat
      721 tgcgttaatt gtcatcgttg tattgcggtg tgtccggtaa agatggcaaa cgatggctca
      781 ggcgatcatg tgcggatcaa cggggattta tgtatcggat gcggagaatg catcgatgcc
      841 tgtacccatg gtgcccgtga gggtattgat gatgccgagg ctttttttgg agccctagcc
      901 cgaagggaaa aaatcgtcgc cattgtcgct ccggcggttg tttcaagttt ccccgatgac
      961 tatttacgct taaacagttt tttagcccag atgggtgtgt ccgcattttt tgatgtgagc
     1021 tttggagcag agttaaccgt aaagtcctat cttgagtata aaaaagaaca caatcccaaa
     1081 accatcattg cccaaccttg cccaacttta gttacgttta ttgaactcta ccgccctgaa
     1141 ctcattccct atcttgctcc ggctgatagc cccatgcttc acaccatcaa gatgattcgt
     1201 gaatactatc ctttgtatag tgcatacaag gttgctgtat tgtctccctg tttttctaaa
     1261 cgacgggaat ttgacgagac gggaattggt gattacaacg ttactttccg ctcccttctc
     1321 gattatattc ggaaaaaggg cgttgtactg tcggagtatc ctgaaaagga gtacgacaat
     1381 cctccagctg agcgtgcggt attattttcc acccctggcg gtttgatgcg gactatagaa
     1441 cgttctgtcg cgggcgcttc tgaaattacc cgcaagatcg aaggtcatcc ccaggtgttt
     1501 catttcctgg ctcatatgag cgaggctatc aagaccgggg ctgcccccct acatcaagta
     1561 gtggattgcc ttaactgcga acttggctgc aatgggggac ctggtaccaa taatcggggt
     1621 aaacatgcgg acgtgataga acagcccatt gaacagcgta atcgcgatat gcaaaagcga
     1681 tatcgcccaa aaggtctttt taagtttttg ggctcaaatc gaaaaaaatt ggatgccctg
     1741 attaaccagt attggaaaaa gggc
//
LOCUS       DAOQXQ010000411         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_81521_length_1759_cov_3.646714, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000411 DAOQXQ010000000
VERSION     DAOQXQ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81521_length_1759_cov_3.646714"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKH81_06990"
     CDS             <1..201
                     /locus_tag="PKH81_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNQ97819.1"
                     /translation="CLLAVSQALRLRIKQGELDSTSLGQIEVLLSHVSKVFPLVEADR
                     PLETELRTTLETVRRREWEVLL"
     gene            198..632
                     /locus_tag="PKH81_06995"
     CDS             198..632
                     /locus_tag="PKH81_06995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ97820.1"
                     /translation="MKQGLITISTDFTVEFYDVDSMQIVWHGNYVKYFEVGRCALLDE
                     IAFGYPEMASSGYAWPVVDIRLKYIRPLQFRQQARVEATLLEYENRLRIAYAIYDRET
                     GSLLTKGESIQMAVHVETMTSCFASPECLTSKVEQYLATGDR"
     gene            679..1293
                     /locus_tag="PKH81_07000"
     CDS             679..1293
                     /locus_tag="PKH81_07000"
                     /inference="COORDINATES: protein motif:HMM:NF039988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNQ97821.1"
                     /translation="MYCALPVQANGPLFSRTPEQGPNSDFNRVLSRLVGPGVVRGKFI
                     QTKNLTSVSRKLTSSGSFLVSRSDGILWDTQKPFPSVLVVGKQGIMQQGASGKIVRMD
                     GDGNPVFAQFSGAMRAVFSGNSSELFSRFEVYFNSDQDVWWIGLIPRDTTVLQVISRI
                     ILCGKDSLDNVDIIEPSGDSVKYAFTHQTFSAGLTDEEKKFFER"
     gene            1265..>1759
                     /locus_tag="PKH81_07005"
     CDS             1265..>1759
                     /locus_tag="PKH81_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97822.1"
                     /translation="MKKKSFLSDSRTGICRRFISAKQYHPLVWLLMHCVPLLFLVVLP
                     VRIESDLYSILPKTQETRGVSLAEAHMSAQSNSGVIVLAGHPDFSRARSIALSLANDM
                     SGITGLDSVVCEVSADLLDPLRSFIHEYRFLLQDKDTLERIRQKPEAFAADALSRIFM
                     PFSVV"
BASE COUNT          428 a          349 c          432 g          550 t
ORIGIN      
        1 tgtcttcttg cagtaagtca ggcattgcgg ctgcgcatta agcagggaga actcgacagt
       61 acatctcttg gtcagattga ggtcctttta tctcatgtgt ccaaggtatt ccctcttgtt
      121 gaggcggaca gacctcttga gacagagtta cgtacaactc tggagactgt gcgaaggcga
      181 gagtgggagg tattgttatg aaacaaggct tgataacgat atccacagat tttaccgttg
      241 agttttatga tgtagactct atgcagattg tgtggcacgg taattatgtt aaatactttg
      301 aagttggacg ctgtgctctg cttgatgaaa ttgcctttgg ttatcctgaa atggcatcaa
      361 gtggttatgc ctggcccgtt gttgatattc ggcttaaata cattcggcca cttcaattca
      421 ggcagcaggc ccgagtagag gcaacccttc ttgaatatga aaacaggctg cgaatcgcct
      481 atgcaattta tgatcgtgaa acaggatcct tgcttacaaa aggggaaagt atacaaatgg
      541 ctgttcatgt tgaaacaatg acaagttgtt ttgcatctcc tgaatgttta acgagcaagg
      601 ttgagcagta tcttgcaacg ggagatcggt gacgtgaggt tatatcgggg acatgtaata
      661 gctgttgtct gctttataat gtattgcgct cttcctgtac aggctaacgg gcctctgttt
      721 tccagaactc cggagcaagg gcctaattcg gactttaatc gagttttatc ccgacttgtt
      781 ggaccaggag tcgtacgggg taaattcatt caaacaaaaa atttaacaag cgtgtcccgg
      841 aagctgacat caagcgggtc ttttttggtc tctcgttccg atggaattct ttgggatact
      901 caaaaaccat tcccttcggt tctagttgtg ggtaagcagg gtataatgca acagggagca
      961 agcggaaaga ttgtccggat ggatggggat ggtaatcctg tatttgcaca attttcaggg
     1021 gcaatgcgtg ccgtgttttc cggtaatagt tcagagcttt tttcccgatt tgaagtgtac
     1081 tttaatagtg accaggatgt gtggtggata ggcttgatcc ctcgcgacac gacagtattg
     1141 caggttattt cacgaattat tctttgtggt aaagatagcc ttgataatgt tgacatcatt
     1201 gaaccaagcg gcgatagcgt gaagtacgct tttacacacc aaaccttttc cgctggatta
     1261 accgatgaag aaaaaaagtt ttttgagcga tagtcgtacc gggatttgtc gcaggtttat
     1321 ttctgccaag cagtatcatc cgcttgtttg gcttttaatg cattgtgtac ctctactatt
     1381 tctggtagtg cttcctgtgc gtatcgaatc agatttgtat tcgatattac ctaaaacaca
     1441 ggaaacacga ggagtatctc ttgctgaagc acacatgagt gcgcaaagta attctggggt
     1501 tattgtgtta gcgggccatc ctgatttttc tcgcgcacga tctatcgctc tttctctggc
     1561 aaacgacatg tccggtatta ccggtcttga ttctgtcgtg tgtgaagttt ctgcggatct
     1621 tcttgatcct ctgcgctcct ttattcatga gtatcgattc ttgcttcaag acaaagatac
     1681 tcttgaacgt attcgtcaaa aacctgaagc ctttgccgcg gatgctcttt cgcgtatttt
     1741 tatgcctttc tcggtggtg
//
LOCUS       DAOQXQ010000412         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82041_length_1753_cov_2.213781, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000412 DAOQXQ010000000
VERSION     DAOQXQ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82041_length_1753_cov_2.213781"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..982)
                     /locus_tag="PKH81_07010"
     CDS             complement(68..982)
                     /locus_tag="PKH81_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ97823.1"
                     /translation="MSSHKHTGTYILAALTIVVWSSTFISTKILLALFTPAEILLYRF
                     TLAWVCMFAVYPRIHRWPKGSLSDTVRATARAEFPYLLAGITGGSLYFLAENYALRFS
                     LASNVSLLVSTAPILTAIIAHFFLPSERLKSTIVSGALIAFAGAALVILNGTFVLRLH
                     PAGDLLALSSALCWAFYSVIIKKTSDGIHPLYLVRKIFFYTILTMLPLCILPSFRSQI
                     RFDFSALANPVMAFNLIFLTLFASCGAYVSWNRVIKALGATRANTFIYFIPLLTLIES
                     AIILKEPLTLFALGGAALILFGVYRSKQ"
     gene            complement(993..>1753)
                     /locus_tag="PKH81_07015"
     CDS             complement(993..>1753)
                     /locus_tag="PKH81_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97824.1"
                     /translation="MTPSQWAALCRFRDAFRQQCEQWLIQGGGTAGWLAKLQQEAAQA
                     NGTPNYSIETPVVYNRNLDCICQEDDIRLILVGDNPGKEEQKNANQRYLVGQAGRLAD
                     GFFSRNSELSIHFRQQVLILNKCPIHSAKTKELAYIKRMGGPAFEHLFQESQIWMARE
                     TAALQHALDCSLWIVGYGELRSKGLFSGYAATLSDCYPNKDAEGPGKDVRLFQHFSMN
                     RFTIDLSASRKEGESLLQSLDRTGIKHRKEILSW"
BASE COUNT          480 a          370 c          480 g          423 t
ORIGIN      
        1 tggataagac tttgtagcat aatgggaagg gccagggtaa acagagatgg atagaaaccg
       61 tcttttttca ttgtttactc cggtacacac caaagagaat aagggctgca ccgccaagag
      121 caaagagggt taggggttcc tttaaaatta tcgcggactc gataagggtg agtaatggaa
      181 taaaatagat aaaggtgttc gcacgggttg cccccagggc tttgataacc cgattccatg
      241 atacataagc cccgcaggaa gcgaagagag tcaggaaaat caagttaaag gccataaccg
      301 gattagcgag cgcagagaaa tcaaatcgaa tttgggagcg aaaagaaggg agaatacaga
      361 ggggcagcat agtaaggatg gtataaaaaa agattttacg tacaagatac agcgggtgta
      421 ttccatcgct cgtttttttt atgataactg agtaaaacgc ccagcaaagg gcggaggaca
      481 gggccagaag atctcctgcc ggatgtaggc gtaacacaaa ggtgccgttt agaataacaa
      541 gggctgcacc tgcaaaggca atcagcgccc ccgatactat tgtgcttttg agtcgctctg
      601 agggaagaaa gaagtgcgca atgatggcgg tcaggatggg tgcggtggac accagcagag
      661 aaacgttgga tgcaagtgaa aagcggaggg cgtagttttc cgcaagaaaa tacagagatc
      721 ctccagttat tcctgcgagc aggtatggaa attccgcacg cgcagtggct cgcacggtgt
      781 cgcttaaaga accttttggc caacggtgta tgcgtggata caccgcaaac atacaaaccc
      841 aggccagagt aaagcgatag agcaatattt ctgcgggggt aaataaagca agcaggattt
      901 ttgtagagat aaaggttgaa ctccacacca caatggtcag cgcggcgagg atgtaggttc
      961 ctgtgtgttt atgtgagctc atgagttttt ccttaccaag agaggatttc ttttcggtgt
     1021 ttgatacctg tgcggtcaag agactggaga aggctttctc cttccttacg ggatgctgaa
     1081 agatcaatcg taaagcgatt catagaaaaa tgctgaaaga ggcgtacgtc tttgcctggc
     1141 ccttctgcat ccttgtttgg ataacagtcg cttaaagtgg cagcataccc ggaaaaaaga
     1201 cctttggaac gtagttcacc atagccgaca atccagagac tgcagtcaag agcgtgttgc
     1261 agagctgcgg tttcacgagc catccagatc tggctttcct ggaagaggtg ttcaaaggcg
     1321 ggtcctccca tacgttttat atatgccagc tcttttgtct ttgccgagtg tatgggacac
     1381 ttattcaata tgagtacctg ttgtctaaaa tgtatggaaa gctcgctgtt ccgtgaaaaa
     1441 aatccgtcag caaggcgacc tgcctgcccc acaaggtatc gttgattggc gtttttttgc
     1501 tcttcctttc ccgggttatc tcctaccagg ataagacgga tgtcgtcctc ttggcagata
     1561 caatcaaggt tacggttata caccacggga gtttctattg agtagttcgg cgttccgttt
     1621 gcctgggctg cttcctgttg tagcttggcg agccagccag cggttccgcc tccctggata
     1681 agccactgtt cgcattgttg gcgaaaggcg tccctgaatc ggcaaagggc tgcccactga
     1741 gagggtgtca tag
//
LOCUS       DAOQXQ010000413         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82158_length_1751_cov_3.295401, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000413 DAOQXQ010000000
VERSION     DAOQXQ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82158_length_1751_cov_3.295401"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKH81_07020"
     CDS             complement(<1..916)
                     /locus_tag="PKH81_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97825.1"
                     /translation="MKTISRGKKPFLLSMISFIAVMLMIVGCGGGGGTGPVVPDTQQP
                     IIHEMKYEGEAGTDITSDSRAAAQGTLYVKASDNQTQSNQLTFDIKNGNGSFTSYTVT
                     KGNDGYYVKEVAMRPQNIYERGNFDGFDATARVRDASGNGTLEKRFFDLALSEQAGRQ
                     AITEAKDNVPISGFDTAGGFAEDQQVDIQGRNVSVDLVGHFTGGDSGWRELSVKYYNI
                     NDTEDAAFQNIVFNQGNVRIARYSYLGGNNRGYFVWLETNLNYSTSKDTLEAALNFIK
                     TDSGLEDDDVTLTAQASPASGGDVRINSG"
     gene            complement(1102..>1751)
                     /locus_tag="PKH81_07025"
     CDS             complement(1102..>1751)
                     /locus_tag="PKH81_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNQ97826.1"
                     /translation="DYRQGSLATDIFFFFYSGHGFYSGGQSYLALSDSTGISVAELKN
                     ALNQINGTKIVIIDACQSGSFTSMVTGRECTPEELAESGEKFALSIMECFEEETNRDL
                     AEKRGQYNSPYEYYVIAGCSTSQSSFESSGFRNGYLSFFLADGMGHVGSSNPTGSFDR
                     SYDADGYGTGGAVNKAITYREWYQYARSKVTGITSAQTVQGNHTSADFVIGNYTN"
BASE COUNT          390 a          465 c          362 g          534 t
ORIGIN      
        1 tcccgctgtt gatccgcacg tctcccccgc tcgccgggct tgcctgcgcc gtgagggtca
       61 catcgtcgtc ttccaggccc gaatcggtct ttatgaaatt taaggctgct tctaaggtat
      121 ccttgctggt actgtagtta agatttgttt caagccatac gaaatatcct ctgttgtttc
      181 caccgaggta cgaatatctc gctattctta cattcccttg attaaataca atattttgaa
      241 aagcggcgtc ttcggtatca tttatattgt agtatttcac gcttagctct ctccaaccac
      301 tatcgccacc cgtgaaatgc ccgactaaat ctactgatac atttctgcct tgaatgtcca
      361 cttgttggtc ttccgcgaat cctccagccg tatcgaagcc agatatagga acattatcct
      421 tagcctctgt tatggcttgt cttccggctt gttcactcaa tgctaaatcg aaaaatcttt
      481 tttctaaagt gccatttcca gaagcgtctc ttactcttgc tgtagcatca aatccatcaa
      541 aattccctct ttcataaatg ttttgtggcc tcattgcgac ttctttcaca tagtacccgt
      601 cattgccttt tgtaaccgta taagatgtga atgaaccgtt accatttttg atatcaaacg
      661 ttaattggtt gctttgcgtt tgattatcgc tggctttgac ataaagcgtc ccttgcgcgg
      721 cggctctgct atctgaggtg atgtctgtcc cagcctctcc ttcgtatttc atttcatgga
      781 tgattggttg ttgcgtatcc gggaccacag gccccgtccc acctccccca ccgcatccga
      841 cgatcattaa cattaccgcg ataaacgaaa tcatactgag taaaaatggt ttttttcccc
      901 ttgaaattgt tttcatcgtt accctccatt cttttaggtt cgtattaagg gtgtttaccc
      961 caactgcatt ctgataccgg aaaattccgt tactatccgc ctaattttca ccacgccttt
     1021 gaagcagaat gaatcgtctg ctcacgattt attatccgtg gtgttatcag ggcttttgat
     1081 gttaaaaaat agccttataa cttaattcgt gtagttgccg atcacgaaat cagcggaggt
     1141 atggtttccc tgcaccgttt gcgcggaggt tataccggtt accttgctgc gggcatattg
     1201 gtaccactcc cgataggtga tcgctttgtt caccgcaccg cccgtcccgt atccgtccgc
     1261 gtcgtaactg cggtcaaaac tgccggtcgg gttcgaggag ccaacatgcc ccatcccgtc
     1321 ggccaagaag aaggacagat acccgttccg aaaacccgag ctttcaaaag aagactggct
     1381 ggtactgcac ccggcgatga cgtagtattc gtaaggcgag ttgtattgcc ctcttttttc
     1441 cgctaagtcc ctgttcgttt cttcctcaaa acattccata atcgataacg cgaatttttc
     1501 tccggattcc gccaactctt ccggcgtgca ttcccttccg gtgaccatcg aggtgaagga
     1561 gcccgattga caggcgtcga taataacgat tttcgtgccg ttgatttgat tgagcgcgtt
     1621 cttcaattcc gctacggaaa tgcccgtcga atcggatagc gccagatacg attgtccgcc
     1681 gctataaaat ccatgcccgc tgtaaaagaa gaagaaaatg tcggtagcca ggcttccctg
     1741 cctgtagtcg c
//
LOCUS       DAOQXQ010000414         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82293_length_1749_cov_3.061393, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000414 DAOQXQ010000000
VERSION     DAOQXQ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82293_length_1749_cov_3.061393"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKH81_07030"
     CDS             complement(<1..1441)
                     /locus_tag="PKH81_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97827.1"
                     /translation="MKRNLIAFGIATLVSGALLASEITVPDLETLIRGSGSAVPTGAL
                     PELSLPKPVVIPEVAPAEPDIPEDTPNVVVTDTPTADIPAPIPPKATSGGIGGHAELG
                     SGSPSVFHGDVLVAQEDGDLPGFTAAFSHDSADGYGTKDSGRGYFDRSTVLSLLFFHI
                     KASHPWHAGITARERSDGLQGLSQDYYSISTRTVDWTGALDFYRNEDAGLRASLSFEG
                     GVYSFAGERPLSATAPVFSVADWRGFRMSPGLSFTASRGEVTAGLHGSYEVESEAEAG
                     DVQSLRADLQLSWLLDPVTLFATAGVFYDSEEGILPPFEAGLRYEREDSLFSKAHISG
                     GLLLTRQDIVDLALEDPFIRKAGLSVYAADWFGKARLDLSIPSGFSAGLGLLWRRTWD
                     KRYTLVSTGTAGVDGLINLDLVSRESLETEASVARAVGIWNFGVSWTAEWMDAPYVDA
                     VHWLTANAEVADRSIHELWALFGDLSVAVD"
     gene            complement(1438..>1749)
                     /locus_tag="PKH81_07035"
     CDS             complement(1438..>1749)
                     /locus_tag="PKH81_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97828.1"
                     /translation="EKTAREERLVFAAAWSLLATLDRQDNAHVRSARAFLSSGSMYAP
                     LDGERAAEALYGAIDSFVQGKKHADAQTTYQTLIASWPDSRWTARAGMLLSAHPTEKK
                     R"
BASE COUNT          446 a          509 c          457 g          337 t
ORIGIN      
        1 catcgacggc aaccgataaa tctccaaaaa gggcccacag ctcgtgtata gaccgatctg
       61 ctacctctgc attggcggta agccagtgca cggcatctac atacggagcg tccatccact
      121 cggccgtcca cgacacccca aagttccaga ttcctactgc gcgggctaca gaggcctcgg
      181 tttccagact ctcccgggaa accaaatcca ggttgattaa cccgtcaacg cccgcagtcc
      241 ccgttgacac aagggtatac cgtttgtccc aggttctacg ccacaaaagg ccaaggcctg
      301 cagaaaaacc ggagggaatc gaaaggtcaa gccgagcctt gccaaaccaa tcggcagcat
      361 atacagaaag acctgccttt ctgataaagg gatcttccag ggcaagatct acgatatcct
      421 ggcgtgtaag taacagtccg ccggatatat gagccttaga aaaaagtgaa tcttcccgct
      481 cgtatctaag tcctgcctca aagggaggga gaatcccctc ttcggaatca taaaaaaccc
      541 cagcagtagc aaacagggtt accggatcaa gaagccatga aagctgcaga tcagccctta
      601 gcgattgcac gtcccccgct tcagcttcgc tttctacctc atatgagcca tgtaagccgg
      661 cagttacctc gccccgggat gcagtaaagg aaagtccggg agacatccgg aaaccccgcc
      721 aatctgcaac ggagaacacc ggtgcggtag cagacaaggg tcgctctccc gcaaaggagt
      781 atacccctcc ctcaaacgaa aggctcgccc gtaaaccagc gtcttcattg cgataaaaat
      841 ctagagcgcc agtccaatcc acggttcgtg tggaaatact gtaataatcc tggcttaagc
      901 cctgtaagcc atcagagcgc tcacgggcgg taatacctgc atgccaggga tgagatgcct
      961 taatatgaaa aaagagcaga gacaacaccg tactccggtc aaaataacct cggcccgaat
     1021 ctttagttcc atatccatcg gcgctgtcat gactaaaggc cgcagtaaaa ccgggtaagt
     1081 cgccgtcttc ctgggctacc aatacatcac catgaaacac tgatggagag ccagagccca
     1141 attccgcgtg ccctccgatc ccgcccgatg tggccttggg tggaatcggt gcgggaatgt
     1201 cagccgttgg agtatccgta accacaacgt ttggtgtgtc ttccgggatg tcgggctctg
     1261 cgggggctac ctccggaata acaaccggct tgggcaagct caactccggc agggccccgg
     1321 tgggtactgc cgatccagag ccccggataa gagtctccaa atcgggaacg gtaatctcgg
     1381 aggcaaggag cgctcctgat accaaggtgg caataccgaa ggcaattagg tttcgtttca
     1441 tcgtttcttc tccgtagggt gcgccgacaa caacatgccg gcacgggccg tccagcggga
     1501 atccggccag gaagcgataa gggtttgata ggttgtttga gcgtcggcgt gctttttacc
     1561 ctgcacaaag ctatcaatcg ctccatagag ggcttctgcc gcgcgctctc cgtccagcgg
     1621 agcatacatg gagcccgagg aaaggaaggc acgggccgag cggacatggg cattgtcctg
     1681 gcggtccagg gtagccagaa gactccaggc agcagcaaat accagtcgct cttcccgagc
     1741 cgtcttctc
//
LOCUS       DAOQXQ010000415         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82314_length_1749_cov_2.602125, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000415 DAOQXQ010000000
VERSION     DAOQXQ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82314_length_1749_cov_2.602125"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKH81_07040"
     CDS             complement(<1..404)
                     /locus_tag="PKH81_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ97829.1"
                     /translation="MASAIVNIFKIPELRRRVLFTLFILIVYRIGAHIPIPGINIEAL
                     KSYFDQAAKSQAGGLVDFFDLFAGGALKRFTVFALGIMPYISASIIMQLLTVVIPQLE
                     RISKEGPEGHKKINQYTRYGTVLLCAIQSLGLT"
     gene            complement(414..878)
                     /gene="rplO"
                     /locus_tag="PKH81_07045"
     CDS             complement(414..878)
                     /gene="rplO"
                     /locus_tag="PKH81_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ97830.1"
                     /translation="MEERYTLKKPESIKNRKRVGRGMSSGHGKTSCRGHKGQMSRSGA
                     KRRAWFEGGQMPLQRRIPKRGFNNPFRLEFAVVNLTQIGSLGSQEIDRDILIKKGLVK
                     GKESAIKILGNGSIEKAITVYADAFSATAKEKIEKAGGKAIVGRPAEQQKES"
     gene            complement(893..1081)
                     /gene="rpmD"
                     /locus_tag="PKH81_07050"
     CDS             complement(893..1081)
                     /gene="rpmD"
                     /locus_tag="PKH81_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNQ97831.1"
                     /translation="MANKIEVKQVRSRNGKKPNQRKTLTALGLRKMNSVRVHDDNPVI
                     RGMIMKVHHLVEVREIKK"
     gene            complement(1095..1610)
                     /gene="rpsE"
                     /locus_tag="PKH81_07055"
     CDS             complement(1095..1610)
                     /gene="rpsE"
                     /locus_tag="PKH81_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ97832.1"
                     /translation="MKVLRKKTKNEGLELVEKVVTINRVAKVVKGGRRFSFSALSVVG
                     DGQGHIGVGFGKANEVPDAIRKSVEAAKKNVYKVNRTRNTIPHEVIGRFKSARVILKP
                     ASPGTGIIAGASVRAVIERAGIHDVLAKVQGSRNPLNVVKATVDGLLQLRSLREAAEM
                     REVPLNKLWEA"
     gene            complement(1623..>1749)
                     /locus_tag="PKH81_07060"
     CDS             complement(1623..>1749)
                     /locus_tag="PKH81_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ97833.1"
                     /translation="LLAGKAKEMGVGKVVFDRNGYLYHGKVKAFADALRENGLEF"
BASE COUNT          362 a          524 c          392 g          471 t
ORIGIN      
        1 gtaaggccga gagactggat ggcgcacagt aataccgtcc catatcgggt atactggttg
       61 atctttttat gaccctcggg cccctccttg gaaatacgct caagctgagg tatgaccacg
      121 gtaagaagct gcatgatgat agaggcggag atatacggca ttatccccag cgcgaagacc
      181 gtaaaccgct tgagcgcgcc gccggcaaac agatcgaaga aatcgacaag cccgccggcc
      241 tggcttttgg cggcctggtc gaaatatgat ttaagcgcct cgatattaat accgggaatt
      301 ggtatatgcg ccccgatccg gtaaacgatc agtataaaca aagtaaaaag aactcttcgg
      361 cggagttccg gtattttaaa gatgttgact atcgcgctgg ccattgggac ctcttagctt
      421 tccttctgct gctccgccgg tctgccgacg atcgctttcc cgccggcttt ttcaatcttt
      481 tctttcgcgg tcgcggaaaa agcgtccgcg taaaccgtta tcgccttttc aatggatccg
      541 tttccgagaa tcttgatagc gctttccttg cctttaacaa ggcctttctt tataagaatg
      601 tccctgtcta tctcctggga accaagtgag ccgatttgcg tgagattaac cacggcgaat
      661 tccagccgga acggattatt aaagcccctt ttgggaatcc ttctctgcag gggcatctga
      721 ccaccctcga accacgcgcg ccgcttcgct ccagatctgc tcatctgtcc cttgtgcccg
      781 cggcagctgg ttttaccgtg accgctgctc attccgcgcc cgaccctctt cctgttctta
      841 attgattcgg gtttcttaag tgtatagcgc tcttccatgt ctcacatgct ctctactttt
      901 taatttccct gacttcaacg aggtgatgta ccttcatgat cattcccctg ataacggggt
      961 tgtcgtcatg cactcggacc gaattcatct tgcgcagccc aagtgccgta agcgtcttgc
     1021 gctggttcgg cttcttgccg ttgcggcttc ttacctgttt cacttctatc ttgttggcca
     1081 tgtacttcac cctgtcatgc ttcccataat ttgttaagcg gaacttccct catttcggcc
     1141 gcctctctga ggctccggag ctgcagcaag ccatcaaccg tggccttcac cacgttgagg
     1201 gggtttctgg atccctgaac ctttgcgagg acatcgtgaa tgcccgcacg ctcaattacc
     1261 gcgcgaactg aagcaccggc gataataccg gtacccggag atgcgggctt tagtataacg
     1321 cgcgccgatt tgaatctccc tatgacctcg tgcggaatgg tattccgtgt cctgttgacc
     1381 ttataaacat ttttcttcgc cgcctctaca ctcttgcgta tcgcgtccgg cacctcattc
     1441 gccttgccga aaccgacacc tatatgcccc tggccgtcac caacgaccga gagagcgctg
     1501 aaagagaaac gcctgcctcc cttcaccact ttggcgacac ggttgatcgt gaccaccttt
     1561 tcaacgagct ccaggccttc attctttgtc ttctttctaa gcaccttcat cggagatcac
     1621 ccttaaaatt ccagaccgtt ttcacgcaag gcgtcggcaa acgcttttac cttgccatga
     1681 tacagataac cattgcgatc gaaaaccacc ttccctacgc ccatctcttt cgccttcccc
     1741 gccaacaac
//
LOCUS       DAOQXQ010000416         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82463_length_1747_cov_2.804965, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000416 DAOQXQ010000000
VERSION     DAOQXQ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82463_length_1747_cov_2.804965"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PKH81_07065"
     CDS             <1..1443
                     /locus_tag="PKH81_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNQ97834.1"
                     /translation="EKALKRANLPYRQAVTHAGSHAGYYPGSFPLALKILYHPDTGAL
                     WGAQAVGPDGVDKRIDVISAILGKEGTVHDLAEFDHAYAPPYSSAKDPVNMVGFIAEN
                     VLDKVSSTITWEELPSYQDKGAFLIDVRSNEEAALGSIPGATVIPQTELRKRLTEIPR
                     DKPIVVFCAVGLRGYLAERVLRQHAFTNVVNLTGGYKTWLAAETERAILEGRTPEPKP
                     LFDKPIVSTEDDGRPIVNEHGSSFTVDACGLQCPGPIMRLKQEIEKISNGDILTVTAT
                     DPGFARDAQSWCTLTGNRLVSIDSTAGRITAVIEKNSTGATQKPSASVGNGSTLIVFS
                     NDLDKALASFVLANGAAASGKEVTMFFTFWGLSVIRKNQKVKAKKDLMGTLFGMMLPR
                     GMKQLNLSSMNFGGAGARMMKGRMKSRGVDQVETMFEQAQKSGVRMIACQMSMDIMGI
                     SETELLEGVEIGGVATYMEAASNSTVNLFV"
     gene            complement(1456..>1747)
                     /locus_tag="PKH81_07070"
     CDS             complement(1456..>1747)
                     /locus_tag="PKH81_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97835.1"
                     /translation="NHGRTTLEIVFGVLEILCGIFILVDAFKRFPAKTSRNVLLAIIA
                     LWIIRIFITQILQGIELSDRGILFQPSFWSWLFTLSMYAVVSVNLWILYRGE"
BASE COUNT          481 a          416 c          437 g          413 t
ORIGIN      
        1 gagaaagcgc ttaagcgtgc caaccttccc tacaggcaag cggtaaccca tgccggaagc
       61 catgccggct attatcccgg ctcattcccc cttgccctta agattctcta tcatccagat
      121 acgggtgcac tctggggcgc tcaggcagta ggacccgatg gcgtagacaa acgaatagat
      181 gttatatctg ccatccttgg taaggaagga accgttcacg atcttgcgga atttgatcat
      241 gcctatgctc ctccctattc aagcgcaaaa gacccggtaa acatggttgg gtttatcgca
      301 gaaaatgtcc tggacaaggt ttcttccacg attacctggg aagagcttcc ctcctatcag
      361 gataaaggcg cgttccttat tgatgtgcgc agcaatgagg aagcagcctt gggttcaata
      421 cccggcgcaa ccgttattcc tcaaacagag ctcagaaagc ggcttaccga aattccccgc
      481 gacaaaccaa ttgtagtgtt ttgcgcagta ggattgcggg gatatcttgc cgagcgcgta
      541 ttgcgacaac atgcctttac caacgtggta aatctgaccg gcggatataa aacatggctt
      601 gctgcggaaa cggagcgggc gattcttgaa ggtcgtacac cggagcccaa gcccctcttt
      661 gacaaaccaa ttgtttctac cgaggatgac gggcgaccta tcgtcaatga acatggctca
      721 tcctttaccg ttgacgcctg cggcttacaa tgccccggac cgatcatgcg cttaaaacag
      781 gaaattgaaa aaatttccaa tggcgacatc ttgaccgtaa ccgcaaccga ccccggcttt
      841 gcgcgggatg cccaatcctg gtgtacgctc accgggaatc ggcttgtttc aattgattca
      901 acggcaggtc gcattaccgc agttatcgaa aagaacagta caggggcaac acaaaaacca
      961 agcgcatcgg tgggcaatgg atcaaccctt attgtctttt ccaacgatct tgataaggcg
     1021 cttgcctcct ttgttttggc aaatggagct gctgcctccg gaaaagaggt cactatgttc
     1081 tttaccttct gggggctgtc ggttatacga aaaaatcaga aggttaaggc aaaaaaagat
     1141 cttatgggga cgctttttgg catgatgctt ccgcggggca tgaaacagct caatctttcg
     1201 tcaatgaact ttggaggggc cggtgcccgt atgatgaagg gaagaatgaa gagtcgaggg
     1261 gttgatcaag ttgaaacaat gtttgaacaa gcgcagaaat ccggagtgcg catgattgcc
     1321 tgtcagatgt ccatggatat tatgggtata agcgaaaccg aactgcttga gggagtagaa
     1381 attggaggag tggcaaccta tatggaagcg gcctcaaaca gtacggttaa tctgtttgta
     1441 taaaagctat ggcttttatt cgcccctata aaggatccac agatttactg acaccacagc
     1501 atacatggac agggtaaaga gccaggacca aaaactgggt tgaaataata taccccgatc
     1561 ggaaagctcg ataccctgca gtatctgggt aataaagatt ctgataatcc acaaagcgat
     1621 tatcgcaagc agaacgtttc gggaggtttt tgcgggaaat cttttaaagg catctaccag
     1681 gataaagatc ccgcacagaa tctccagcac gccaaataca atttccaggg tggttctgcc
     1741 atgatta
//
LOCUS       DAOQXQ010000417         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82476_length_1747_cov_2.459811, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000417 DAOQXQ010000000
VERSION     DAOQXQ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82476_length_1747_cov_2.459811"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="hcp"
                     /locus_tag="PKH81_07075"
                     /gene_synonym="priS"
     CDS             <1..747
                     /gene="hcp"
                     /locus_tag="PKH81_07075"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNQ97836.1"
                     /translation="FLFTTNCLMPVRPSYADRVFTTSVVAYPELVHIPLVNGKKDFSP
                     VIKKAKELGGYPEGKKMTGINGGSTLTVGFGHGTVLSVADTVIDAVKQGAIKHFFLVG
                     GCDGAKPGRNYYTEFVQKTPQDTVILTLACGKYRFNDMDLGTIGGLPRIMDMGQCNDA
                     FSAIKVAVALAEAFKCEVNDLPLSLVLSWYEQKAVCILLTLLYLGIKNIYLGPTLPAF
                     VSPNVLKVLVEKWNVTPISTAEADLKKILG"
     gene            830..>1747
                     /locus_tag="PKH81_07080"
     CDS             830..>1747
                     /locus_tag="PKH81_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97837.1"
                     /translation="MKKNHLALTVTMISIGMILAVYILMQIIAFFRDNAILGLTSLAA
                     LPGSVVLFIAMYVLPPMLICGTLLYRYARPLEKILDRLRAGETLDEHTAEQTRVRMLR
                     FSFILYILNMIGFVLGYVILVIIEDSPSGLITPFRLLILVTNITGALIYASAQASLTN
                     IAFGELRDRLELREIGKRKREMSRKKRQIIVGVSLVIYTLTFMEFNGQMADKATNLAL
                     SVITAQRQGLIMEEDVRDAYITGIPAILPSVLSRPGYNPDEVILPWETPGHAVRSKQI
                     VFFLHAFFILLVAALVHTTGALEVSDQLKA"
BASE COUNT          417 a          470 c          402 g          458 t
ORIGIN      
        1 tttcttttta ccacaaactg ccttatgccg gttaggccgt cctacgcaga tcgcgtgttt
       61 accacatccg tcgttgcata ccccgaactg gtccacatcc cgcttgttaa tggtaaaaaa
      121 gatttctccc cggtaatcaa aaaggccaaa gaacttggcg gttatcccga ggggaaaaag
      181 atgacgggca taaacggtgg ctcaacactc actgttggct ttggccacgg aaccgtgctc
      241 tctgttgcgg acacggttat cgacgcggta aagcagggag ccattaagca cttcttcctg
      301 gtgggcggtt gcgacggggc aaaacccggc cgaaactact acaccgagtt cgtgcaaaaa
      361 acgccccaag acaccgttat cctcaccctt gcctgcggta agtaccggtt taacgacatg
      421 gaccttggaa ccattggggg attaccccgc atcatggata tgggtcagtg taacgatgca
      481 ttcagtgcca taaaggtagc agtcgcgctc gcagaagcct ttaagtgcga ggttaatgac
      541 cttccactgt cccttgttct gtcctggtac gaacaaaagg ctgtgtgtat cctgctaacg
      601 ctgctgtatc tgggtatcaa gaacatctat cttggcccga cacttcctgc atttgtgtcg
      661 cccaatgtac ttaaggtttt ggtagaaaaa tggaatgtaa ctcccatttc aactgcggaa
      721 gccgatctta aaaagatact cggataacta ccatcgcgct tacaaattaa gggaaaggct
      781 ctgtgccttt ccctttgtat aatgctatac tattcggtaa ggaaacacca tgaagaagaa
      841 ccaccttgcc cttaccgtca ccatgatttc tattggcatg attctggctg tgtacatact
      901 catgcagatc attgcctttt ttcgggacaa cgccattctg gggctcacct cgcttgcagc
      961 ccttcccggt tccgttgtgt tgtttattgc gatgtacgtt ctccctccca tgcttatatg
     1021 cggcacactg ctctatcgct atgcgcgtcc cctggagaaa atcctggatc gtttacgcgc
     1081 aggcgaaaca cttgacgagc acaccgccga acaaaccaga gtgcgtatgc tcaggttttc
     1141 ctttatcctg tacatactta acatgattgg cttcgtcctg ggctacgtga ttctggtcat
     1201 aattgaagat agtccttccg gccttattac cccctttcga ctgctcatcc tggtaacaaa
     1261 cataaccggc gctctcattt atgcttcggc ccaggcctcc cttaccaaca tcgcctttgg
     1321 agagttacgg gaccgccttg agctaaggga aattggaaag agaaaacgcg aaatgagcag
     1381 aaagaagcgc cagattattg tgggggtgag ccttgttatc tataccctta cctttatgga
     1441 gtttaacggg caaatggcag acaaggccac aaacctggct ctctcagtta ttaccgctca
     1501 acgtcagggc ttgattatgg aagaggatgt acgtgatgcc tacataaccg gcatccccgc
     1561 cattctcccc agcgtcctct cgcgtcccgg ctataatcca gacgaggtta tccttccctg
     1621 ggaaactccg ggacacgcag tgcgatcaaa acaaatagtc ttttttcttc atgctttttt
     1681 tatcctgctc gttgcggccc ttgttcacac caccggagcc ctggaggtaa gcgatcagct
     1741 taaggcc
//
LOCUS       DAOQXQ010000418         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82504_length_1747_cov_1.630024, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000418 DAOQXQ010000000
VERSION     DAOQXQ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82504_length_1747_cov_1.630024"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="rsgA"
                     /locus_tag="PKH81_07085"
     CDS             <1..928
                     /gene="rsgA"
                     /locus_tag="PKH81_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNQ97838.1"
                     /translation="LVLGGTNNQFEVECPDNLVRICGIKGKILKESQGYYNPLAPGDR
                     VSIVPDENDAHQGQIHSLIPRRNHFVRFNQKGKSPQMLAANLDLIVIVTTPAEPPFRP
                     RFVDRLLVQAEAEHIEPLILVNKCDLAPDADLAERLSDWERLGYSVLQVSAQTGFGLD
                     ILESRLKGRLSAFVGQSGVGKSSILNALDASLQLRTGGLCEKYDRGSHTTVKGMLFHL
                     PLSSGGSADIVDTPGVRRFVIHDIQSQDLALYFPEMEGLVGQCSWGLSCSHQSEAGCN
                     ILEAVYSGVILEQRYESWRRIKEEIETGNWSD"
     gene            949..>1747
                     /locus_tag="PKH81_07090"
     CDS             949..>1747
                     /locus_tag="PKH81_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97839.1"
                     /translation="MNQRSLDILEYPRIREEIAGFCMSEEGRNALMSAEPLCDSQEIE
                     RRKTLGRAWMRALSAETPPRLTGWPAISSFITALKVEGASLHTEECYALGLFCASSNG
                     LREWASMREKNSGSGRIDVLSQTAGTDPLVQGGDALLVTSLLALPDLSLAEQAIFRII
                     DRNTGEIRDLPELRAIRQEIRKIRQDIDRLVGSYLSDDSLKTALQSNLPTVRDGRQVI
                     AVRANYKGRIKGIVHEVSQSGQTVYLEPTDVVDRNNDLVSEESRLARE"
BASE COUNT          410 a          434 c          480 g          423 t
ORIGIN      
        1 acttgtcctt ggcggaacca acaatcaatt cgaggtggaa tgtccggata atcttgtgcg
       61 gatatgcggg ataaagggca agatacttaa ggaatcccaa gggtattata atccccttgc
      121 acccggagac cgtgtatcca tcgtgcccga cgagaatgac gcacatcagg gacaaattca
      181 tagccttatt ccccggcgca atcattttgt tcgctttaat caaaaaggca aaagcccgca
      241 aatgcttgcc gcgaatcttg acttgatcgt tatcgttacc actccggccg aacctccttt
      301 tcgtccccgg tttgtagacc gcctgttggt gcaggcagag gctgaacata ttgaaccgct
      361 tattctggtg aataagtgcg atcttgcccc tgatgccgat ttggcggagc gattaagcga
      421 ttgggagcgt cttgggtatt cggtgcttca ggtttcggcc caaaccggct tcgggctcga
      481 tatccttgaa tcccgactca agggccggct ttcggccttt gtcggccaat ctggtgttgg
      541 taaatcgagt attcttaacg cccttgacgc ttccctgcag ctgcgaacgg ggggcctttg
      601 cgaaaaatac gaccgggggt cccacaccac cgttaagggc atgctttttc atctcccgct
      661 ttccagtgga gggtcggcag atattgtaga cactccggga gtgcgtcgct ttgttatcca
      721 cgatatacag agccaggatc tggctctgta ctttcccgag atggaaggcc tggttggcca
      781 gtgttcttgg ggcttgtcct gttctcatca aagcgaggca ggatgtaata ttcttgaggc
      841 cgtgtattcg ggagttatcc tggagcagcg ctatgaaagc tggcggcgca tcaaagaaga
      901 gatagaaacc ggtaactggt ccgattaggg acaattgaag agagtattat gaatcaacga
      961 agccttgata tacttgaata cccgcgtatc cgcgaagaga tcgcaggctt ttgcatgagc
     1021 gaggaaggcc gcaatgcgct tatgtctgcc gagccgctgt gcgatagcca ggagatagaa
     1081 aggcgaaaaa cactgggaag ggcctggatg cgcgccttgt ccgccgaaac tccgcctcgt
     1141 cttaccgggt ggcccgctat cagttctttt ataacggctc ttaaggtaga aggagcttcc
     1201 ttgcacacag aggaatgcta tgccctgggt ttgttttgcg cttcttccaa cggactgcgg
     1261 gagtgggcct ccatgcgcga aaaaaacagc ggctccggaa ggattgatgt gctttctcaa
     1321 accgccggta ccgaccctct tgtccaaggc ggcgatgcac ttcttgtaac atccttgctg
     1381 gccctaccag atctctctct cgctgaacag gcgatattca ggataatcga tcgtaatacg
     1441 ggggaaatcc gcgatttacc ggagctacgg gcaattcgcc aggagatacg aaagattcgc
     1501 caggatatcg atcgattggt gggatcatat ctgtccgacg acagtttaaa aacagccttg
     1561 caatccaacc tgcctacggt gcgggacggc agacaggtaa tcgcggtgcg tgctaattat
     1621 aagggacgca ttaagggaat cgttcacgag gtgtctcagt ccggtcagac tgtatacctt
     1681 gagcctacag atgtagtaga ccgaaacaat gatcttgtct cggaggaaag tcgactagcc
     1741 cgcgaga
//
LOCUS       DAOQXQ010000419         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_82688_length_1744_cov_3.059207, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000419 DAOQXQ010000000
VERSION     DAOQXQ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82688_length_1744_cov_3.059207"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PKH81_07095"
     CDS             <1..757
                     /locus_tag="PKH81_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ97840.1"
                     /translation="TIIIAGASVDDVFAITLFSVFLSVVTGGISSYVMTAVTVPLSIV
                     RGVLCGALAGLILSLIFKRHYATIRATEKALILLVSSMLLVAVGETLHFAALLGLMTM
                     GFILRERHERVGHELSGKLSKIWVFAEIVLFVMIGFSLDPSVALAAGVKGLLVISGGL
                     VFRSLGVLLATYGSSLSLKERLFCTIAYLPKATVQAALGGVALSQGITEGHQILAIAV
                     IAILFTAPLGLVGIRFFGNRWLALETEAGLEHK"
     gene            808..1407
                     /locus_tag="PKH81_07100"
     CDS             808..1407
                     /locus_tag="PKH81_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ97841.1"
                     /translation="MNKTLTQAIKDRRSIYALGKKEVVSQQRITELVEFTLTHTPSAF
                     NSQSARILLLFDKESSRFWSLVTATLESLVPPSDFPKTKEKIQGFDSGYGTILFFEDQ
                     SVVEGLQKSFPLYADNFPVWSLQSNGMLEYGLWIALQAEGLGSSLQHYNPLVDEAVRS
                     TWQLPESWKLLAQMPFGSPEGLPGDKEFSPLEYRLRVAQ"
     gene            complement(1478..>1744)
                     /locus_tag="PKH81_07105"
     CDS             complement(1478..>1744)
                     /locus_tag="PKH81_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97842.1"
                     /translation="TENVTTAPEDVRAMQLLTIINTEKNDLIKKDLLTSAKKLNSRFS
                     NLIDEALTALEPQDASGSGDSQEFVEPSIEEETEGGPVEEDYVE"
BASE COUNT          404 a          382 c          421 g          537 t
ORIGIN      
        1 aacgataatt attgcaggcg catcagttga tgatgtgttc gccataactc ttttttcggt
       61 ttttctgtca gtagtgaccg gaggaatctc cagttatgtg atgacggctg taacagtgcc
      121 tttatccatt gtaagaggag ttctttgcgg ggcactggcg ggtcttattc tttctcttat
      181 atttaagagg cactacgcta ccatacgagc aacagaaaaa gccctgattc ttctggtatc
      241 ctcgatgctt cttgtggccg ttggcgaaac ccttcatttt gcggctttac ttggattgat
      301 gaccatgggt tttatcttgc gtgaaaggca tgagcgggta ggccatgaat tgtccggtaa
      361 gctttccaag atatgggtat ttgccgagat tgtgcttttt gtcatgatcg ggttttccct
      421 tgatccttcg gttgccctgg ctgccggggt aaaaggactt cttgttatta gcgggggcct
      481 tgtttttaga tcgctcgggg ttttacttgc tacttatggt tcttcgctat ctttaaaaga
      541 gcggcttttt tgtactattg cctacctgcc caaggctacc gttcaagcag ccctgggagg
      601 agtcgctctt tcgcagggta taaccgaggg gcatcaaatt cttgctatcg cggttatagc
      661 aattttgttc acagctccgc tgggattggt tggtatacgc ttttttggca atcgctggtt
      721 agccttagaa acagaagccg gtcttgaaca taaataagtt gaccttcctt aacaaaatat
      781 gtaccttgat actagtgagg tatgcttatg aacaagaccc taacacaggc gattaaagat
      841 cgacgcagta tatacgcact tggaaaaaaa gaagtggtat cccaacagcg gattaccgaa
      901 ttagttgagt ttacactaac ccatacccct tccgcgttta actcccaaag cgcacgtatt
      961 ctcctacttt ttgacaagga aagctctcgg ttctggtctt tggtaacggc gaccctggaa
     1021 tccctggttc ctccttccga ttttccaaaa accaaagaga agatacaagg ctttgacagt
     1081 ggatatggaa ccattctttt ttttgaggat cagtctgtcg tagaaggatt acaaaagtcc
     1141 tttcctttgt atgctgataa ttttccggta tggtcgcttc agtcgaacgg aatgctggag
     1201 tatggacttt ggattgcctt gcaggccgaa ggactaggtt catctcttca gcattataat
     1261 ccactggtag acgaggctgt tcgctccacc tggcagctgc cagagagctg gaagctcctt
     1321 gcacaaatgc cgtttggatc gccagaaggc cttcccggcg ataaagaatt tagtccgctt
     1381 gagtatcgct tgcgggttgc tcaatgatgg aagttgaata aaaaaaaccg ctcgtacgag
     1441 cggttttttt cctttgtgca caactggtgc gctccggtta ttctacatag tcttcttcaa
     1501 ccgggccacc ctctgtttct tcctcaatcg aaggctcgac aaattcctgg gaatccccgg
     1561 agccagaagc atcctggggc tccagggcag ttaatgcctc gtcaataagg ttagaaaatc
     1621 gggaattgag ttttttagcg cttgtaagca aatctttttt tataagatca tttttttctg
     1681 tgttgataat ggtaaggagc tgcatagccc ggacatcttc aggagcggtg gtaacgtttt
     1741 cggt
//
LOCUS       DAOQXQ010000420         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_83160_length_1738_cov_3.379085, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000420 DAOQXQ010000000
VERSION     DAOQXQ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83160_length_1738_cov_3.379085"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKH81_07110"
     CDS             <1..648
                     /locus_tag="PKH81_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNQ97843.1"
                     /translation="EETGYSFPSGHSQNSAAFWLCAISALTGKKKYALLLLPLCIGLS
                     RICLGLHYPTDVLAGWIAGSLIALVVLTVLPPLARFILLRAPILKEEPVAQRMPLILA
                     AAAALAFNGFSGGDTSMGGLLFGMVAARYVLEKKGLASKITAQEGRLAVKAARLGLGL
                     VVLAGLYLGLRQVAPGAQSPHYTLFRFLRYALVGAWVTGGAPALFLRCGLSGTRQ"
     gene            complement(617..1480)
                     /locus_tag="PKH81_07115"
     CDS             complement(617..1480)
                     /locus_tag="PKH81_07115"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ97844.1"
                     /translation="MKGIIVAAGYGTRFLPVTKTIPKEMLPLVTVPSIQFIVDEFVAS
                     GVTDIVIISSRRKKVLEDYFDREVELEAVFTAEGKTAQLEKIRPPAANISFVRQQRML
                     GTGHALLQVRPWIGNEPCIVAYPDDLHFGKVPLSAQLVKEWETSGASVMASITVEGDV
                     SRYGVLDLAEDGRHVRGIVEKPRAGTEPSHEVSIGRYLYTPEFFDLLEQGWKEHCAEA
                     EKQGKSAGEYFHIYALNRLMSAGKVVYCHAEGERLDTGEGAGYLGAILRYADTQSDLR
                     AVIDEYLKVRT"
BASE COUNT          409 a          490 c          417 g          422 t
ORIGIN      
        1 gaagaaaccg gatattcatt cccctcggga cacagtcaaa actccgcagc cttttggctc
       61 tgcgctatct cggcccttac gggaaaaaag aaatacgctc ttcttctttt gcctctgtgc
      121 attggactaa gcaggatatg ccttggactg cactacccaa ccgatgtact ggcaggctgg
      181 attgcgggaa gtcttatcgc cctggtagtc cttaccgttc ttccccctct tgctcgattc
      241 atcctgttac gcgctccaat cctgaaagaa gaacccgtag cgcaaaggat gccccttatc
      301 cttgccgctg cggcagcctt ggcctttaac ggattttccg gaggggacac ctctatggga
      361 ggactcctgt tcggcatggt agccgctcgc tatgtgttgg aaaaaaaagg ccttgccagt
      421 aaaattactg cacaagaagg aaggcttgcc gttaaggcag ctcgtcttgg ccttggactc
      481 gtggtattgg caggcctcta tctggggctt cgccaggtag cccccggcgc acaatccccc
      541 cattacacct tgtttcgctt tctgcgatac gccctggtag gcgcttgggt aacgggagga
      601 gctcctgcgc tctttctcag gtgcggactt tcaggtactc gtcaataacc gctctaagat
      661 cgctttgtgt gtcggcatag cgaagaatgg cgcccaggta acctgctccc tcgccggtat
      721 caagccgctc tccctccgca tgacaataca ctacctttcc ggcggacatc aggcggttaa
      781 gtgcgtagat gtggaaatat tctccggcac tcttaccctg tttttctgcc tcggcgcaat
      841 gttccttcca tccctgctcc aagaggtcaa aaaactcggg agtatataaa tacctgccga
      901 tggaaacctc gtgggagggc tctgtccctg ccctgggttt ttccacaatc ccccgcacat
      961 gtcgaccatc ctctgcaaga tcaagcaccc cgtagcgaga cacgtctcct tctacagtga
     1021 ttgacgccat aacagaggcg ccagaagtct cccattcttt aacgagctga gcggatagag
     1081 gaaccttgcc aaaatgaagg tcatccggat aggcgactat acaaggctca ttgccaatcc
     1141 aggggcgaac ctgtaacagc gcgtgtccgg tccccagcat ccgctgttga cggacaaaag
     1201 agatgttggc ggcaggaggt cgtatttttt caagttgagc ggtttttcct tcggcagtaa
     1261 acaccgcttc cagctcaacc tcccgatcaa agtaatcctc caacaccttt ttccggcggg
     1321 aactgataat cacaatatcc gtaaccccgg aggcaacaaa ttcgtccaca ataaactgaa
     1381 tagacggaac agtaaccaag ggaagcattt ccttgggaat tgttttggta accggtaaaa
     1441 agcgagtgcc ataacccgca gcaacaatta ttcctttcat gccaggtagt gtagcattcc
     1501 cgctgtccaa cggcaagaag ggcctttttt cttgactgga gcatgttttg ccttcaataa
     1561 tgaaggtact gcacaacgat accgacagtt tctttccccg cattggagga caaaaagtga
     1621 caaataccgt aatagagctg ctccatctgg gagcccaacg ctttctggac atgccctacc
     1681 ttggcgaaaa acgcgatagt ggctggaccc ataccagttt tgttgaagca gaccgact
//
LOCUS       DAOQXQ010000421         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_83197_length_1738_cov_2.282234, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000421 DAOQXQ010000000
VERSION     DAOQXQ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83197_length_1738_cov_2.282234"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKH81_07120"
     CDS             <1..202
                     /locus_tag="PKH81_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97845.1"
                     /translation="YQDTSVIIEDIIRKVSNGSIIPVRLGKGEGSRDDYLFNRIDLLI
                     SALLEEGFEIVPVDTLILNERK"
     gene            complement(186..1610)
                     /locus_tag="PKH81_07125"
     CDS             complement(186..1610)
                     /locus_tag="PKH81_07125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNQ97846.1"
                     /translation="MLNSKSLFGAAKAALISVFTLSALSLVLLIYANNRLEVVVAPLP
                     DSQAAIVRSLYQQSSGTNKQDSSLYPLLKQSRWIGPEPTLIPSRKALAQDVHARSAIL
                     ISADTGEILFEKNADISIPPASMTKLVAMYTALKAVESGEIQLDQEITPPEESWAQNI
                     PAGSSLMFLGPDQKVTIRELLEGMAVVSGNDAAIALAMTLCGSVDSFVERMNRETAAL
                     GLHSTSFVEPSGLSEYNRTTSREFAAFSRLYINRYPQTLSAFHSRRELVYPMPHNTKA
                     PTEVQGIRQSATNKLLESLDGCDGLKTGFIYESGFNLALTCMRNGQRFIAVFMGGPGS
                     NSREGNRFRVQDGSSVMEWAFSNWRHTTLEYPDTIPIPVWGGSEDGLHVLRVFDTPAL
                     IPVLADGIPTDGNTLVPHLKVPPYLEAPVIAGTIIGTIDWEMNGKVVLQEDVIADRTI
                     TVGSAGERCIDFLALQAVRLISVR"
     gene            complement(1597..>1738)
                     /locus_tag="PKH81_07130"
     CDS             complement(1597..>1738)
                     /locus_tag="PKH81_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97847.1"
                     /translation="LDLRRYGTVPHSGFGLGFERLLLYVTGMGNIRDVIPFPRAPKLA
                     EF"
BASE COUNT          445 a          407 c          440 g          446 t
ORIGIN      
        1 atatcaagac acttcggtta ttatcgagga tattatcaga aaggtttcca atggatcgat
       61 aattcccgtt cgccttggta agggtgaagg tagccgggat gattatcttt ttaatcggat
      121 tgatttactt attagcgcct tattggaaga gggctttgag attgtaccgg tagataccct
      181 tattcttaac gaacggaaat aagtcgtact gcttgcaagg ccaaaaaatc aatacatctt
      241 tccccggcgc ttccaacagt tattgttcgg tcagcgataa catcttcttg caagaccacc
      301 ttcccgttca tttcccaatc tatggtccca ataattgtcc ctgcaattac gggcgcttct
      361 aaataaggcg gaaccttgag gtgcggcacc agcgtgttcc catcggttgg tataccatct
      421 gccaggacag gaatcagagc tggtgtatca aaaaccctta ggacgtgtaa gccatcttcg
      481 gagcctcccc ataccggtat gggaattgtg tcaggatatt ccagtgttgt atgtcgccaa
      541 ttagaaaatg cccattccat aacggacgaa ccgtcttgaa ctctaaaccg gttaccctct
      601 ctgctgtttg acccggggcc acccataaag acggcgataa accgctgtcc atttcgcata
      661 caggtcaaag ccagattaaa cccggactca taaataaaac ctgttttcaa gccatcacag
      721 ccgtcaaggg attcaagtag cttgtttgtg gcggattgcc tgatcccttg tacttcggtt
      781 ggcgctttgg tgttatgagg catgggatat accagttccc ggcgggaatg aaaagcgctt
      841 aacgtctgtg gataacggtt gatatacagg cgtgaaaagg ctgcgaattc ccgcgatgtg
      901 gtacggttat actcactcag tccggaaggc tcaacaaagc tggttgagtg taaaccaaga
      961 gccgcggttt ccctgttcat tcgttccaca aaggaatcta cagagccaca aagggtcatg
     1021 gcaagggcaa tggccgcatc gtttcccgat acaacagcca tgccttccaa taattctcga
     1081 atcgttactt tttgatcagg gcccaaaaac atcagcgagg accctgcagg tatgttttgt
     1141 gcccaagact cttctggggg tgtaatttct tgatcaagtt gtatctcccc agattcaaca
     1201 gccttgagcg ctgtgtacat agcgaccagt ttggtcatgg atgcaggcgg tatcgagatg
     1261 tctgcgtttt tttcaaacag tatttccccg gtatcagcgg atatcagaat tgccgagcgg
     1321 gcatgcacat cctgggcaag ggctttgcgc gagggtataa gggttggctc ggggccaatc
     1381 catcggcttt gtttcagaag cgggtaaagg cttgagtcct gcttattggt accgcttgat
     1441 tgttgataaa gagagcggac aatagctgcc tggctatctg gtagcggtgc gacaacaact
     1501 tccagacggt tgtttgcata gatcagcagc acaagagaca gggcagaaag ggtaaagaca
     1561 gaaatcaggg ccgccttggc ggccccgaaa agggatttag aattcagcaa gctttggtgc
     1621 cctcgggaag gggattacat cccggatgtt tcccatgccg gttacataca gtaacagacg
     1681 ttcaaagccg agtccaaaac cggaatgagg taccgtgccg tagcgtctaa gatcaagg
//
LOCUS       DAOQXQ010000422         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_83589_length_1733_cov_3.350417, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000422 DAOQXQ010000000
VERSION     DAOQXQ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83589_length_1733_cov_3.350417"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKH81_07135"
     CDS             <1..850
                     /locus_tag="PKH81_07135"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNQ97848.1"
                     /translation="IVISAESTQLAPEKGAGTITSDHLCFPAKLVHRHILDLVAKGVD
                     RIFFPLIKFEAVQNIKENSFNCPVVTGYPDLIYNAINPEEQYNIPLDKPSFSFRNKKL
                     LLQKCRHYFASQGVSSRQVHTAFLSAQKKQQSFEQTLRSKTNEIIERTAKKASTLFVL
                     AGRPYHVDPLLNQGVPEIINSFNHEYVLAEYLPLDTDIPPQATPSSWAFTNRLYKAAH
                     FVAGRERTEMIFLHSFSCGPDGVCIDAIKKILATSGKKLTVIKLDDMQSNDAIRLRIR
                     TLMESL"
     gene            863..>1733
                     /locus_tag="PKH81_07140"
     CDS             863..>1733
                     /locus_tag="PKH81_07140"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNQ97849.1"
                     /translation="MIHTQQADSANPPILIPLLSSFHSPLLPALLRRFNINGIILPEP
                     DSESEQLGLRYVNNDICYPAILVVGDILKALFSGKYERTTISVGISQTGGQCRLSTYK
                     EIIEDALQAAGFQDIPVVSLAFSLKGSGNTKHAKKAIKFGTFSMLHAMHRTLLLGDAF
                     MTMYCAMAVREKHAGESEALLARYIQLAQHITSDKAILDLLSKAIADFNAVEERSYTH
                     IPKIGVVGEIYVKLCPHANRHIYKWLIERGVHPVVANSSYFMPQMLVNKKINHDQRLL
                     FLSGIQRLALALIQ"
BASE COUNT          501 a          423 c          356 g          453 t
ORIGIN      
        1 tatagttatt tccgcagagt ctacccaact cgcccctgaa aaaggcgccg gcaccataac
       61 ctccgatcac ctgtgctttc ctgcaaagct cgtgcaccgc cacatcctgg accttgtggc
      121 aaagggcgtg gatagaatat ttttcccgct tataaaattt gaagcagtac aaaacataaa
      181 agaaaacagc ttcaactgtc ctgtagtaac cggatatcca gaccttattt ataacgcaat
      241 caatcctgaa gaacagtaca acatcccgct cgataaaccg agcttttcgt tcaggaacaa
      301 aaagctcttg ttgcaaaaat gccgccatta ttttgcatca cagggcgtgt cgtcccggca
      361 agtacacacg gcattcctgt ctgctcaaaa aaaacaacaa tcctttgaac agacattacg
      421 atccaagacc aacgagatta tcgagcgaac agcaaaaaaa gcttcaaccc tttttgtcct
      481 ggcgggccgc ccctatcatg ttgatccttt actaaaccag ggcgtgccgg agattatcaa
      541 ctcctttaat catgaatatg ttttggcgga gtatcttccg ttggatacag acataccccc
      601 acaggcaacc ccttcttcct gggcctttac caatagattg tacaaggccg ctcactttgt
      661 tgccggcaga gagcgcaccg agatgatttt tctgcactcg ttttcttgcg gaccggatgg
      721 agtatgtatt gatgcaataa aaaaaatact ggctacaagc ggcaagaaac ttacggttat
      781 caaactggac gacatgcaaa gtaacgacgc gatacggctt agaattcgaa ccctgatgga
      841 atctttatag aaggacttta ccatgataca tacacagcaa gcagacagtg ctaatccacc
      901 tatactcatt ccactcttgt catcgtttca ttctccctta ttgccggctt tactcaggcg
      961 cttcaacatt aacggaatta tcttacccga accggacagt gaatcggagc aacttggtct
     1021 taggtatgta aataacgata tctgttatcc ggcaatatta gttgttggtg acatcttaaa
     1081 ggctcttttc tccggaaagt atgagcgcac tacaatcagt gtcggcatca gccagaccgg
     1141 gggccaatgc cgcttaagca cctataaaga aattatcgaa gatgccttac aagcggccgg
     1201 gttccaggac attccggttg tgtctcttgc tttttcccta aagggaagcg gaaacacaaa
     1261 acacgccaag aaggcaatca agtttggtac attcagcatg ctacatgcaa tgcaccggac
     1321 cttgttgttg ggcgacgctt ttatgaccat gtattgtgct atggcggttc gcgaaaaaca
     1381 tgccggggaa tcagaagccc tacttgcaag gtacattcaa cttgcacaac acattacctc
     1441 ggacaaagct attcttgatc tgctgtccaa ggcgatagca gattttaatg cggtagaaga
     1501 aagaagttat acacacattc ctaaaatcgg tgttgtcggc gagatttatg ttaagctttg
     1561 tccccatgca aaccgtcata tttataaatg gttaatagaa aggggtgttc atccggttgt
     1621 ggcaaactca agctatttta tgccccagat gcttgtaaac aaaaaaataa accatgacca
     1681 gcggcttctt ttcttatccg ggattcaaag gcttgccctt gctcttattc aaa
//
LOCUS       DAOQXQ010000423         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_83788_length_1731_cov_2.664081, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000423 DAOQXQ010000000
VERSION     DAOQXQ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83788_length_1731_cov_2.664081"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKH81_07145"
     CDS             complement(<1..244)
                     /locus_tag="PKH81_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNQ97850.1"
                     /translation="MKLTPRVIAMDLDDTLLKHDLSISDRTVDTLNRCADRGIIIMLA
                     SGRAPEAMYQYAERLGLHTRSSYLICHNGSHAIESDT"
     gene            300..>1731
                     /locus_tag="PKH81_07150"
     CDS             300..>1731
                     /locus_tag="PKH81_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ97851.1"
                     /translation="MTILLAILCLVLTTLLILAARRLKSCSDMLKDATGPVSASLKIL
                     RNCISTFASGDLRVRAVMPAERSRSAAGRVLDELVRTGLTDFNTITDIPSRRVCFSGA
                     NSYHEGKIAGMRIAEILGGKGSVACIIPFYNQINHVLRMKGCLNYLEDQYPQIKTLGI
                     FEGAGNREVSIQRTEEILAANKNLDILYVTDGHTPPSVVETIEKLGRKEVKVVAFDAM
                     PENIALLKAGKISCLIEQNSFAQAFNALVHLYNAREAGWRPLTPKLFMQPISIDLRNY
                     RTYWDDETNSRIMMDEERADLAIPEPNKSGSRYRFALIMPLSTGFFEGLGRGAAAAKK
                     TLAEYGVEVEVIDAFHEWADFGSASIFNPIIERFVREGYDGFATVVVDPPIVYSINDA
                     VAAGLTVTTFNTEPSSFREIVLNMINNVENLADNSQSLAAAAEQSARATAQIGSSIDG
                     IKEYIHEQKNRIAASDTELKGLNQMIG"
BASE COUNT          427 a          403 c          459 g          442 t
ORIGIN      
        1 tggtgtctga ttctatggcg tgagacccat tgtggcagat cagataactt gatcgggtat
       61 gaaggccgag ccgttcggca tattgataca tggcttccgg ggcgcggcct gaggcgagca
      121 taatgataat cccccggtcg gcgcagcgat tgagggtatc taccgtacgg tccgatatag
      181 aaagatcgtg cttgagtagg gtgtcgtcca aatccatggc aataacgcgg ggtgtaagct
      241 tcatgccggt gagtatatcc catttgattt attggagaaa gagcgttacc atgaggctga
      301 tgacaatact actagctatt ctttgcctgg tgttaacaac cttgctcata cttgcggcca
      361 gaaggcttaa aagctgttct gacatgctta aggacgcgac cggaccggta agcgcgagtc
      421 ttaagattct tcgtaattgt atttccacct ttgcttcggg tgatcttcgc gttcgggcgg
      481 ttatgccagc cgaacgctct cggtctgctg cggggcgggt tctggatgag ctcgttcgta
      541 ccggattaac cgattttaat accattaccg atatcccttc ccggcgcgtg tgtttttcgg
      601 gagctaacag ctaccatgaa ggcaagattg ccggcatgcg cattgccgag attcttgggg
      661 gcaaggggtc tgtcgcctgt atcattccct tttacaatca aataaatcat gtccttcgca
      721 tgaagggatg ccttaactat ctggaagatc aatatcccca gattaaaacc ctgggcatct
      781 ttgagggtgc gggaaaccgc gaggtgtcga ttcagcgtac agaggagata ttagcggcga
      841 acaagaatct tgatattctc tacgttaccg atgggcatac tcctccgagt gtggtagaga
      901 caatagaaaa gttgggcagg aaagaggtaa aggttgtcgc ctttgacgcc atgccggaga
      961 atattgcctt gttaaaggcc gggaaaatat cgtgccttat agagcaaaat tcctttgccc
     1021 aagcgtttaa cgccctggtt catttgtata acgcacggga agcaggatgg cggccattaa
     1081 cgcccaagct ctttatgcag cccatttcca tcgatctgcg taattaccgc acctattggg
     1141 acgacgagac taatagccgc attatgatgg atgaggaacg agccgacctg gcaatcccag
     1201 agcccaacaa gagcggaagc cgatatcgct ttgcccttat catgccgctt tccaccggct
     1261 tttttgaagg tcttggccgc ggagctgcag ccgctaaaaa aacccttgcc gaatatggcg
     1321 tagaggttga ggttatagac gcctttcatg aatgggcaga ctttggctct gcctcaatct
     1381 ttaatccaat aatcgagcgc tttgtacgcg aggggtatga tggttttgcg actgtagttg
     1441 ttgatccccc cattgtgtat agcatcaatg atgctgttgc tgccggtttg acggtaacca
     1501 cgtttaatac tgaaccttcg agcttccgcg agattgtact aaatatgatc aataacgtgg
     1561 aaaatcttgc ggacaacagt cagtctcttg ccgccgcagc agagcagtct gcccgggcta
     1621 cagctcagat cggaagctca attgacggaa taaaagaata cattcacgag caaaagaatc
     1681 gaatcgccgc aagcgacacc gagcttaaag ggcttaatca gatgattggc g
//
LOCUS       DAOQXQ010000424         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_83881_length_1730_cov_2.299701, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000424 DAOQXQ010000000
VERSION     DAOQXQ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83881_length_1730_cov_2.299701"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKH81_07155"
     CDS             <1..238
                     /locus_tag="PKH81_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNQ97852.1"
                     /translation="IVGRKAVVGRPFMYGTTKDFLERFGLNDISDLPKVEDMSELLGF
                     DWNSGPAMTLKGISTRPECDVQARLLQPLYYPRL"
     gene            244..594
                     /locus_tag="PKH81_07160"
     CDS             244..594
                     /locus_tag="PKH81_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNQ97853.1"
                     /translation="MTLEDAYILKPEDIVSRLGGLPSNKIHCSVLGDKALRAAIDDYL
                     ERNKLPPLTGRESVEVICTCLNVTDRDIEEAYKAGDRSWEDLQARTKIGTACGGCKER
                     TLELLHELEHLYGE"
     gene            complement(607..1473)
                     /locus_tag="PKH81_07165"
     CDS             complement(607..1473)
                     /locus_tag="PKH81_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNQ97854.1"
                     /translation="MNRITVATLALLAGSFFASLFCLEWPVSEIYIERIFGQRAGKTI
                     EQGILFNQTDIVRSSGNGDILCVLEQNHNMYGFPGTLGNAVIVSHEDGLLSVYGNLDS
                     HERIKDRVTVESGTILGSSGKTGYAKPGDLIFQVLDQRRLIALNPLLLLPSLADTKAP
                     TIKNVIAVSSSEQSLSLGSIKSVRQGSYRIFADISDTFDKNGKNVAPFRVTLLLNGRE
                     YHAVPFELIKSEHGLVFLGSDQYVTSRLYGDPERIFLGEINLTRGKNELSILARDTAG
                     NERGAVFTVQTE"
     gene            complement(1470..>1730)
                     /locus_tag="PKH81_07170"
     CDS             complement(1470..>1730)
                     /locus_tag="PKH81_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97855.1"
                     /translation="DTKKLISHIVPLSGKVLSTGDGSLDKVFLVLSQVDTTYTLTAIE
                     WPNHSVASTSFSSHNAFLIQREHTFYLGLDNSISRFDIRGVQ"
BASE COUNT          453 a          389 c          434 g          454 t
ORIGIN      
        1 gatcgtgggc cgcaaggccg tggtcggtcg accgttcatg tacggcacca ccaaggactt
       61 cctcgagcga ttcggcctca acgacatttc cgatctgccg aaggtcgaag acatgtccga
      121 actgctcggc ttcgattgga acagcggtcc cgccatgacg ctcaagggga tctcgacgcg
      181 accagagtgc gatgtgcaag cgcgcttgct tcaacctcta tattatccga ggctataaaa
      241 ggaatgaccc ttgaggatgc gtatatactt aagccggagg atattgtttc ccgattgggt
      301 ggattaccgt caaacaaaat tcattgttct gtgttaggtg ataaggcatt gcgagcggct
      361 atcgatgact atcttgagcg caataaactg ccgccgctaa ccggaagaga atccgtcgag
      421 gttatttgta cctgccttaa tgtaaccgat cgcgacatag aagaagcgta caaggcagga
      481 gaccggagtt gggaagatct tcaggcgcga acaaagattg gaaccgcctg tggtggctgt
      541 aaggagcgaa ccctggagct actacatgaa ctggaacact tgtacggaga gtaagtatct
      601 ttttttttat tcggtttgta cggtgaatac tgcgcctcgt tcattccccg cagtgtcccg
      661 tgcaagaatt gatagctcgt tttttccccg ggtcagattt atttctccca gaaagatacg
      721 ctccggatct ccgtagagtc tgctggtaac atattgatcg cttcctaaaa ataccaggcc
      781 atgttcactt ttaatgagct caaagggcac ggcatgatat tcgcggccgt ttagtaacag
      841 cgtaacccga aagggagcaa cgttttttcc gtttttatcg aaggtgtcgc taatatctgc
      901 gaagattcgg tagcttccct gacgtactga tttaattgac ccaagggaga gactttgctc
      961 tgaagaagaa actgcgataa catttttaat agtcggggct tttgtatctg caagggatgg
     1021 caataacaga agcgggttca gtgcaattaa tcgtcgttga tcaagtacct ggaagatcaa
     1081 gtctcccggt ttagcgtatc ctgttttacc tgatgatcca agtatcgttc cgctttctac
     1141 agttactctg tccttgatgc gttcgtggga atccaggttt ccatacacag agaggagccc
     1201 atcttcatgg gaaacgatta ccgcattgcc cagtgtcccc ggaaagccat acatgttgtg
     1261 attttgttca agaacacaga gaatgtctcc gtttccggat gatcgcacaa tgtctgtctg
     1321 attaaaaagt attccctgtt ctatggtctt tcctgcacgt tgaccaaaaa ttcgttcgat
     1381 ataaatctcg cttaccggcc attccaggca aaaaagggaa gcaaagaagc tacctgctaa
     1441 taatgccagg gtggcaactg taatacggtt cattgaaccc ccctgatgtc aaaacgtgaa
     1501 atactgttat caaggccaag atagaaggtg tgttctcgct ggatcaggaa ggcattgtga
     1561 ctggaaaaag aagtggatgc aactgaatga tttggccatt caattgcagt gagcgtatag
     1621 gtggtatcaa cttgagaaag aacaagaaac accttgtcca gggagccatc tccggtagaa
     1681 agaaccttgc ccgacagcgg cacaatgtgg gagatcagtt tttttgtgtc
//
LOCUS       DAOQXQ010000425         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_84253_length_1725_cov_2.953892, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000425 DAOQXQ010000000
VERSION     DAOQXQ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84253_length_1725_cov_2.953892"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKH81_07175"
     CDS             <1..834
                     /locus_tag="PKH81_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97856.1"
                     /translation="LFVHVDRVNADKFPSVTLELRVEDRNRRPVVGLKDANFLITEGQ
                     RPVITPRLLGAGFMQDALDVTVILDRSPASAARLQDIRAALADISRAMKGKGTIRVVS
                     AGSLPVLEGSSPSSAPIWSSFTPRTQVARDWKFDVALRLAVNDLINASARRAVIFLSC
                     GEVGDLSFNRYALNDLASYLGNNGVLFTTIYLTQGQAVSEFEYLCAATSMRSWYLYRS
                     EGLAETIHSLLTSPNGSYQLSYVSSLPTDFGKAYLPVEVEVYLMNRSGRDETGYFSPL
                     E"
     gene            complement(837..>1725)
                     /locus_tag="PKH81_07180"
     CDS             complement(837..>1725)
                     /locus_tag="PKH81_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97857.1"
                     /translation="TPSWTRSAITEGQTTAGGDYLKDSETLLSDISRMDFLVETPPIA
                     DLCMDDLGSTIRDDRSEASRTFINRYAIDWSRSSQSRLSDIWTPSSLGTFIMRETMTD
                     AVSLEVPDTWTAGVKAGFNAINIAGNYSRLALFPWYTQDEISQIYTWSSKWGTQYYTW
                     SIDVWHSLLLFFETDATISIENTFHYDSPSQAGQGELTRDSIAFIWKRPIDHSFLTAL
                     VRLWTDWELTSRREDSIRITVSAGEQLTAQAGWDHTLATGIGKNGEVAVFGGFIWNRY
                     DKEMSDLSIRLGCRGKVMY"
BASE COUNT          391 a          415 c          438 g          481 t
ORIGIN      
        1 ctttttgtgc atgtggacag ggtaaatgcg gacaagtttc cctctgttac ccttgaactg
       61 cgcgttgaag accgtaatcg ccgtcctgtg gtaggtctaa aggacgccaa ttttttaatt
      121 accgaaggtc agcgcccagt cataacgccg agacttcttg gcgcagggtt tatgcaggat
      181 gctcttgatg taactgttat actcgatcgc tcaccggcat ctgcggcacg attacaggac
      241 atacgtgcag cccttgccga tattagccgg gctatgaagg ggaaggggac catacgcgtt
      301 gtgtccgcag gctctcttcc tgtgctggaa ggttcaagcc cttcgtctgc acccatatgg
      361 tcatccttta cgccacgaac ccaggttgcc cgcgactgga agttcgatgt agcgctgcgt
      421 cttgccgtta atgatttgat taacgcctct gctcgcagag cggttatttt tttgagttgc
      481 ggtgaagtcg gtgatctttc ttttaaccgc tacgctctca atgatctagc ttcttacctt
      541 ggcaataatg gggttttgtt tactactatt tatttgaccc agggccaggc ggtaagcgag
      601 tttgagtatt tgtgcgccgc aacatcaatg cgttcttggt atttataccg cagcgaaggg
      661 cttgccgaaa cgatacactc cctgctgacc tcgccaaatg gaagttatca gctttcgtat
      721 gtgtcgtccc tgcccacgga ttttgggaag gcatatttac cggttgaggt tgaggtgtat
      781 ttgatgaatc ggtctggacg ggatgaaacg ggatattttt ctccgcttga atagccttag
      841 tacataacct ttccgcggca tccaagtcta atgctcaggt cggacatttc cttgtcgtac
      901 cgattccaga taaagccgcc aaacacagcg acttcgccat tcttgccaat tccggttgca
      961 agggtatgat cccaaccagc ttgcgcggtc aattgttctc ccgcgcttac cgtaatgcgt
     1021 atggaatcct ccctcctgga agtgagttcc caatctgtcc ataatctgac cagggccgtc
     1081 aggaatgaat gatctatcgg acgtttccaa ataaaggcga tgctgtctcg agtcagttct
     1141 ccttgaccgg cttgagaagg ggaatcgtaa tggaaggtgt tttcgatgga tatggtcgca
     1201 tcggtttcaa agaacaacaa gagcgaatgc catacatcaa tgctccaggt gtaatactgt
     1261 gtaccccatt ttgaggacca ggtatatatc tgcgagattt catcctgagt ataccagggg
     1321 aacagggcga gtcggctata attgcccgcg atgtttattg cgttaaaacc agcttttaca
     1381 cccgcagtcc aggtgtccgg cacttcaaga cttactgcat cagtcatagt ttctctcatg
     1441 ataaaagttc ccaatgatga aggagtccat atatcgctga ggcgggattg ggaagagcgt
     1501 gaccagtcaa tggcgtaccg gtttataaag gtacgcgagg cttcgcttcg atcatcgcga
     1561 attgtacttc caagatcgtc catgcataga tctgcaatgg gtggggtttc tacaaggaaa
     1621 tccatgcgcg atatatcgct caagagcgtt tcgctgtctt taagataatc cccaccggca
     1681 gtggtctgtc cctctgtaat ggcgcttctt gtccaagagg gagtg
//
LOCUS       DAOQXQ010000426         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_84490_length_1722_cov_1.862627, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000426 DAOQXQ010000000
VERSION     DAOQXQ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84490_length_1722_cov_1.862627"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PKH81_07185"
     CDS             complement(<1..1291)
                     /locus_tag="PKH81_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ97858.1"
                     /translation="MDQHSTVALEGVSLVYESPGDEAGGIVTFTALDEVDIIFQSGSI
                     HVLLGENGAGKSTLVHILSGLLTPTRGRLSLNGEPLYLKGPKDALRRGIAMVHQRPLL
                     CDELSALDNTLLSASGFLLQRKTATEQVRRINAEWGSELNLSVKAKNLSPSDRLKTAL
                     FGALLQDPAFLILDEPTAVLSPADRTSFMEAVQKEAARGRGIILITHHLEEAVRWADR
                     ITVLKKGRVKLTENKVFNQEAILAVMRAEIATEGNQGLWTDTPEEKSASGAPCTFAVK
                     DLCAVDGNRPPLVNFSLTAHKGGITGIFGYPGSPLGTLEDALCGMISLTSGTVTVNGS
                     CGRIELSRHTLTPRALRACSVAMVPSNRAFRGSNPVLTIQDILRATDTEYGTPSTKEG
                     IPAQIQRLAGTLSGGQLQRLIVAKELSSKPHIIILCEP"
     gene            complement(1304..>1722)
                     /locus_tag="PKH81_07190"
     CDS             complement(1304..>1722)
                     /locus_tag="PKH81_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ97859.1"
                     /translation="GNWYDASRGSDLERAMQNAGCDVIMPISGGANQGVIAAAKELSF
                     YIAWFDDNGYGKAPSLVISSAIMAQEKLVHEQVAAYINGTLKKGVHTTVGMTDGYIAF
                     VSEDERYIETVPLALRQQMKAMLARIADGSLALPSR"
BASE COUNT          368 a          443 c          477 g          434 t
ORIGIN      
        1 ccggttcgca cagaataata atgtggggtt tgctggaaag ctcttttgcc acaatgagcc
       61 gttgtaattg ccctcccgac agggttcccg ccaagcgttg tatctgtgcg ggaatgcctt
      121 cttttgtact tggggtacca tattccgtat ctgtggcgcg cagtatgtcc tgtattgtta
      181 gcacaggatt ggaaccgcga aacgctctat ttgagggtac catggcgact gagcaggcgc
      241 gtagagcgcg tggtgtaagt gtatggcggc ttagctcaat gcgaccacag gagccattga
      301 cggtaactgt tccgcttgtt agcgagatca taccgcacag cgcgtcttcc agtgtaccaa
      361 gcggacttcc tggatagcca aagataccgg taataccacc tttgtgggcg gtaagtgaaa
      421 agttgacaag gggagggcgg tttccatcta ccgcacaaag atctttgacc gcaaaggtac
      481 acggcgcacc gcttgccgac ttctcttccg gtgtgtccgt ccagagtccc tgattgccct
      541 ctgtcgctat ttctgcgcgc ataacagcaa ggatggcttc ctggttaaac accttgtttt
      601 cggtgagctt gacgcggcct ttttttagta cggtaatccg gtctgcccag cgtaccgctt
      661 cttcaagatg gtgggtaatc aggatgatgc cccgtccccg agcggcttct ttttggactg
      721 cctccataaa tgaagtgcga tcggcagggg agagtaccgc cgttggctca tcaaggatta
      781 agaaggcggg gtcttggagt aaggctccaa agagggctgt tttaagccgg tcggatgggg
      841 ataggttttt tgcctttacc gacagattga gttcgcttcc ccattcggca ttgattcgtc
      901 ttacctgctc tgttgcggtt ttcctttgta gcaagaaacc gctcgccgac aacagggtat
      961 tgtccagggc agaaagttcg tcgcaaagaa gcggacgttg gtgaaccata gcgattcccc
     1021 gtctcagcgc gtcttttggc ccttttagat aaaggggttc tccgttaagg gagagtctcc
     1081 cgcgtgttgg ggtcagaagg cccgaaagga tatgaaccag ggtggacttt cccgccccgt
     1141 tttctcccag gagcacatgg atagatcccg actggaaaat gatatcaacc tcgtccaggg
     1201 cggtaaaggt gacaatacct cccgcctcgt ctccggggga ttcatatacc agggaaacac
     1261 cttccagggc aacggttgag tgttggtcca tcgtatggcc gaattatcgg gatggaagag
     1321 cgagcgatcc atcggcaatg cgggcgagca tcgccttcat ttgctgtcgc aaagcaagag
     1381 gaaccgtttc tatgtatcgc tcgtcctcgc tgacaaaggc gatatacccg tctgtcatgc
     1441 caacggtggt gtgaacaccc tttttgagcg ttccgtttat gtaggcagcc acctgctcat
     1501 gaacgagttt ttcctgtgcc atgatggcac ttgatatgac aagggaggga gcctttccat
     1561 aaccgttgtc gtcaaaccag gctatataaa aggacagctc cttggccgcc gcgataacgc
     1621 cctgattcgc ccctcccgag attggcataa taacgtcgca tcctgcgttt tgcatcgctc
     1681 gctcgaggtc tgagccacga gaggcgtcat accagttacc ca
//
LOCUS       DAOQXQ010000427         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_84676_length_1719_cov_3.715745, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000427 DAOQXQ010000000
VERSION     DAOQXQ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84676_length_1719_cov_3.715745"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..>1719
                     /locus_tag="PKH81_07195"
     CDS             225..>1719
                     /locus_tag="PKH81_07195"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNQ97860.1"
                     /translation="MTDIQQILRENDLLKAQLLLKEEQLLRKNERILYLERQLFGRRS
                     EKKLPDYGEAQLSLFNPEQGKPALEQEVSVLTTLVEDIKQKAEQRRIMQKQHSASGKR
                     SYKIPLHIERRETILEPENVNPDTLVKIGEDVSERLMLEPSRFWVERIVRPVYKAKQT
                     EQTLTTTIFQAPSKPVILPGCIAGESLLSQIIVDKFLYHLPEYRQAKRFKELEVEIPT
                     SSINRWVHVLADKLYGLYAAQMERVLSGNYIQVDETTHPITDRPGSARKGYIWAVRSV
                     LFPGVFFYYDKGSRSQEVVLKMLKGYQGALQTDGYAAYSIYEEKEGVLPLGCMAHVRR
                     KFEQALTTMPEAKPALDYIALLYMLEGNLKAEGADHEQIRREREEKAYPILQQMEIWM
                     QQAYNNCTPKSPLGKAISYAFGMWPRISRYCKEGYFQIDNNGVENAIRPLVVGRKNYL
                     FSGNDSGAEDNCIFYTLLGSCLQAGVDPHKWLTSTLEKIPTLQTPINW"
BASE COUNT          515 a          379 c          419 g          406 t
ORIGIN      
        1 gggagcgagg aggctttgtg atttattaca agcgcatgga aagcggacgc atcagtccca
       61 agatttttct caaagaagga gttggttttc gtaccattcg ttgggatgaa ctggtacttt
      121 ttatggaagg gattagtgta aaaataaagc gaagaaaacg gtataatttt cagtaaaaca
      181 gttgtgtatc tcgttgtaaa ttagtatctt tacattgtaa aacaatgaca gatatacaac
      241 aaatactacg cgaaaacgat ttgctgaaag cgcaacttct cttgaaagag gaacaacttc
      301 ttcgcaagaa tgagcgtatt ctttatttag agcgccagct gtttggtcgg cgttcggaga
      361 agaaattgcc cgattacggt gaagctcaat tgtcattgtt taatcctgag cagggaaagc
      421 ccgccctgga acaggaagtt tcggtgctga ccactttggt agaagacatc aagcaaaagg
      481 cagagcagcg ccgtattatg caaaagcaac attctgcatc cgggaaacgt tcctacaaaa
      541 tcccgctcca tattgaacgc agagagacta ttctggaacc tgagaatgta aatccagaca
      601 cgttggttaa aatcggtgaa gatgttagcg aaagattaat gcttgaacct tcccggtttt
      661 gggtagagcg tatcgttcgc cccgtataca aggcaaaaca aacagaacaa accctgacaa
      721 ctactatttt tcaagcccct tcaaagcccg tgatattgcc gggatgtata gccggtgagt
      781 cgttgttaag ccagatcatt gttgataaat ttctttatca cctgcccgaa taccgacaag
      841 ccaaacgctt caaagaactg gaagtagaaa tacccacttc gagtattaac cgctgggtac
      901 acgtcctggc agataagctc tatgggctgt atgcggcaca aatggagcgg gtattgtcgg
      961 gcaactacat tcaggtagac gaaacaaccc atccgataac cgaccgaccg ggttcggcac
     1021 gaaagggata tatctgggca gttcgctccg tactctttcc cggcgtgttt ttttattatg
     1081 acaaaggatc ccgctctcag gaggtagttc tcaaaatgct taaaggctat cagggagcac
     1141 tgcaaacgga tggttatgcc gcttatagca tctacgaaga aaaagaggga gtgcttccgc
     1201 ttggttgcat ggctcatgtg agacgaaaat ttgaacaggc actcacaact atgcccgagg
     1261 caaagccggc gctcgattac attgccctgc tctatatgct ggaaggcaac ctcaaggcgg
     1321 aaggtgcaga ccacgaacaa atccgcagag aacgcgaaga gaaagcttac ccgatattgc
     1381 aacaaatgga gatttggatg cagcaggcat acaacaactg tactcccaaa agcccgctgg
     1441 gcaaagccat cagctatgct ttcggcatgt ggccacgtat cagccggtat tgtaaagaag
     1501 ggtatttcca gatagacaac aatggtgtgg aaaatgcaat tcgcccattg gtggtgggac
     1561 gcaagaatta cctgttctca gggaacgaca gcggtgccga ggataactgc atattctaca
     1621 cgctgctggg cagttgcctg caagcaggtg tcgatccaca caagtggtta acttccacac
     1681 tcgaaaaaat accgaccctg caaactccaa taaactggg
//
LOCUS       DAOQXQ010000428         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_84862_length_1717_cov_2.237665, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000428 DAOQXQ010000000
VERSION     DAOQXQ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84862_length_1717_cov_2.237665"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKH81_07200"
     CDS             <1..627
                     /locus_tag="PKH81_07200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ97861.1"
                     /translation="LEHKGIAREIRDIRNEFALTDDVRVIATVANFRKVKNHLFLLNG
                     FKVLLKELENVKLLLIGQGTDGDPDNSECDVRSYIEENGLCDKVICTGYRNDVKELLS
                     IADVFCLTSYREGLPISMLEAMSMGLPVVGTNVPGIRDVTMHEKNGYLIEMHDCVALK
                     RTLFTLLTNEQLRRRYGQAAKKVVEDYYSLDQCVGRYQDLFLRLMQKS"
     gene            781..>1717
                     /locus_tag="PKH81_07205"
     CDS             781..>1717
                     /locus_tag="PKH81_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ97862.1"
                     /translation="MVSKKSLGAYCVQSKKAVFLSLSIIFVIACLLRIAPIVYEEWKN
                     PGWHAANINEMEFYYDDVARSLLVGKGFVHSVSPRSPDYKFSFEPGTPFHFVPPLYAW
                     WLYIIYFIFGPNVFIAKVIQCFLDASVCIFIYLLGKRIFEEDKKALLSAVLYAIYPLA
                     IIMCSNLYYQIPLNVALCIVILGYMASVNWKNGFWTGVAVGVSALAKPVTLPFLLLLP
                     AIRLAETGFKKALFKPALAWSVVFILAGLAVLAPWTIRNYIVFKKFVPVQHGGEEVFV
                     QGSKEAYIDLDVNALRQKYPTGLAPSNQLTETAIRN"
BASE COUNT          512 a          287 c          388 g          530 t
ORIGIN      
        1 ctcgaacata agggtatagc gcgggaaata agggatatta gaaatgaatt tgcactaacc
       61 gacgatgtta gggttattgc tacggtagca aatttcagaa aggtaaaaaa ccatttgttt
      121 ctgctaaatg ggttcaaggt acttctcaaa gaattggaaa acgtcaaact gttattgatc
      181 gggcagggca ctgatggtga tccggataac tctgagtgtg atgttcgtag ctatatagaa
      241 gaaaatggat tgtgcgataa agtcatttgt acaggttaca gaaacgatgt aaaggaatta
      301 ttgtctatcg cagatgtatt ttgtctcaca tcgtataggg agggcttgcc gataagcatg
      361 cttgaagcaa tgagtatggg tcttcctgta gttggtacaa atgtacctgg catacgcgat
      421 gttaccatgc acgaaaagaa cggttatctt attgaaatgc atgattgtgt tgcactaaaa
      481 aggacattgt ttacacttct tacaaacgag caactgagac gtaggtacgg acaggcagcg
      541 aaaaaagtcg tcgaggatta ttattcatta gatcaatgcg tcggtcgtta tcaggatctt
      601 tttctcagat tgatgcaaaa gtcataaaat gtgtcaaagc gcgattgcga gataaaaata
      661 cttgtaaata gcaatattgg tcaagataca tattcagaat gtaatcgttc agtaacgatt
      721 agagtattat tatctattag atacttaaag aaatagataa tagccttcaa gaggtcttaa
      781 ttggtaagca aaaaatcctt gggagcatat tgtgttcaat caaagaaggc tgtctttctt
      841 tctttgtcga ttatatttgt tatcgcatgc cttctcagaa ttgcgccgat tgtttatgag
      901 gagtggaaga atcctggctg gcacgcggcg aatataaatg aaatggaatt ctattatgac
      961 gacgtagcaa gaagccttct agtcggtaag gggtttgtgc actcggttag cccccgttca
     1021 cctgattaca aattttcatt tgagccggga acaccttttc attttgttcc gcctttgtat
     1081 gcttggtggc tgtatataat ctatttcatt tttggtccca atgtatttat tgcgaaagta
     1141 atccagtgtt ttcttgatgc ttcagtatgc attttcatct atcttttagg aaagaggata
     1201 ttcgaagaag ataaaaaagc attattgagc gcagtattat atgcaattta tccgttggcg
     1261 ataataatgt gctccaattt atattatcag attccgttaa atgttgctct atgcattgta
     1321 atattgggct atatggcctc ggttaactgg aaaaatggct tctggacagg agtggcggtc
     1381 ggagtatccg cgcttgcgaa gccagtgact ctaccatttt tacttttgtt gccggcaata
     1441 agattagctg aaacaggatt taaaaaggca ttattcaagc ctgcattagc ctggagtgta
     1501 gtattcattc ttgcaggact cgcagtattg gcaccttgga ctattcgaaa ctatatagta
     1561 ttcaagaaat ttgttcctgt acaacatggc ggcgaagagg tctttgtgca aggatctaag
     1621 gaagcgtaca ttgatctgga tgttaatgct ctcagacaaa aatatcccac gggtcttgca
     1681 ccctcgaatc agctaactga aacggcaatc agaaatc
//
LOCUS       DAOQXQ010000429         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_84869_length_1717_cov_2.060770, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000429 DAOQXQ010000000
VERSION     DAOQXQ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84869_length_1717_cov_2.060770"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..553)
                     /locus_tag="PKH81_07210"
     CDS             complement(140..553)
                     /locus_tag="PKH81_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97863.1"
                     /translation="MKMLPHTHPFAIVWKIIILVASLVFLFFSTLTFVFPSGYSGWNK
                     ILHAALAVIFLADIGMQFIMTVKTGHITLEQRKEVRKHYLAKWFYLDLIAALPFDIIG
                     VLWFFDGATPALGDALGHVQFLACRKLTCNGGELG"
     gene            complement(599..>1717)
                     /locus_tag="PKH81_07215"
     CDS             complement(599..>1717)
                     /locus_tag="PKH81_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ97864.1"
                     /translation="LATAIFLGMFAGAVFWGRLSDKIGRRLGFILTIAFDSLFGLLSA
                     FAPSFSIFLILRVCTGFGVGGTLPVDYGMFAEYLPEKKRASRLVILESFWAIGTLAAA
                     ALAWLIVPRFGWRALFAVSAIPGLLLFAIRRGVPESPRYLFSRGKAKEAWAVLAQVAQ
                     ENGTTLPEGELEMSTTTRTWKMRDLFSVDLRRTTIILWVVWFLISIGYYGTFTWLPSW
                     FRVKGFALPSVYPNTVIMALAQIPGYFSAAALVYYWGKKNTLSVYLVASGACAWLFAL
                     ASTPTMVVVSAVALSFFALGAWGALYACTPEAYPTGIRTTGIGAASGWTRIAGVIAPT
                     IGTLVAGESLVLPLAVFALAYVVAGGSSFFLKEERQIR"
BASE COUNT          540 a          438 c          396 g          343 t
ORIGIN      
        1 ggtaaacgca atttgaatat tgatctcatg atcgggatga tagtctccat atccaatagt
       61 agacacagtc gttaccaccc aatacaggga ccgaagatat tgctcatcgt ttctccggct
      121 actctccgaa cccccaatac taacccaact caccgccatt acatgtaagc ttgcggcaag
      181 ccaaaaactg aacatgacca agcgcatcac cgagggcagg tgtagcgcca tcaaaaaacc
      241 aaagaacgcc aataatatca aagggaagtg cggcaataag atccaggtaa aaccattttg
      301 ccagataatg cttacgtact tcttttctct gctccagtgt aatgtgcccg gtttttaccg
      361 tcataatgaa ttgcatacca atgtccgcaa gaaaaatgac ggctagagca gcatgcagaa
      421 tcttgttcca tcctgaatat cccgaaggaa acacaaaagt gagtgtggaa aagaaaagaa
      481 agaccagaga ggcaacaagg attatgattt tccatacaat agcaaaagga tgagtatgag
      541 ggagcatctt catgtaccta gtatcggtct tttttgctgc tccctcattg ctcttatatt
      601 atcttatctg cctttcttcc tttaagaaga aactggatcc acctgcgact acatacgcaa
      661 gggcgaatac cgcaagaggg agaaccaggg attctccagc tacgagtgtt ccaatggtag
      721 gagcgataac accagctatt cgagtccatc cactggctgc cccaatacct gtggtccgaa
      781 tacccgtggg atacgcttcg ggagtgcatg catacaaggc accccaggca ccaagggcaa
      841 aaaaggaaag ggcgacggcg gatacaacga ccatcgtagg cgtagacgca agggcaaaga
      901 gccaggcgca cgccccgctt gccacaagat atacactcaa ggtgttcttc ttgccccaat
      961 aatacaccaa agccgctgct gaaaaataac cgggaatctg agccaaggcc atgatcaccg
     1021 tattgggata caccgaaggt aaggcaaatc cctttaccct gaaccagctt ggaagccacg
     1081 taaaggtccc ataatagccg atagaaatta aaaaccacac gacccacaaa atgattgtcg
     1141 ttcttcgcaa atcaacagaa aaaagatcgc gcatcttcca tgttcgggtt gtggtcgaca
     1201 tttcaagctc gccctctggc aaggtggttc cattttcttg tgcaacctga gcaagtaccg
     1261 cccaggcttc cttggccttt ccccgagaaa aaagataccg gggagactcg ggaacgcctc
     1321 gcctaatggc gaataaaagt aagccgggaa tcgccgaaac ggcaaacagg gctcgccatc
     1381 caaaccgggg aacaatgagc caggctaagg cagcggcggc aagtgtaccg attgcccaaa
     1441 aagactcaag gatcacaagt cgggaagcgc gttttttctc tggtaaatac tcggcaaaca
     1501 ttccgtagtc aaccggtagg gttccgccta cgccaaagcc ggtacatact cgtaaaatca
     1561 ggaagatact aaaggaggga gcaaaagcag aaaggagtcc aaaaagagag tcaaaggcaa
     1621 tggtaaggat aaagccgagc cgacgcccaa ttttatcaga aagtctaccc cagaacaccg
     1681 cgcccgcgaa catacccaag aagattgcgg tagcaag
//
LOCUS       DAOQXQ010000430         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_85151_length_1713_cov_2.899879, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000430 DAOQXQ010000000
VERSION     DAOQXQ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85151_length_1713_cov_2.899879"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1713
                     /gene="ileS"
                     /locus_tag="PKH81_07220"
     CDS             <1..>1713
                     /gene="ileS"
                     /locus_tag="PKH81_07220"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ97865.1"
                     /translation="GLLYEGHYILPYCPRCSTVLSNHELNLGGYKDVHDPAITVKFRV
                     TGTIKASEAERSGLNASDSKPTFLLAWTTTPWTLPSNLGLTLGPDVDYVMVEDSGERY
                     ILAEARLSAYYKDPASVSIVWKKKGSELAGITYEPLFPYFKDLATLEDGRPGAFRTFT
                     ADFVTTEDGTGIVHTAPGFGEDDNKVFKGTGVPTVCPVDAECRFTAEVGDYAGVFVKD
                     ADKAILERLKAEGRLVKREQILHSYPHCWRCSSPLIYRAVGSWFVAVEQIKENLLKAN
                     FQITWQPAHIKEGRFGKWLEGARDWAISRNRFWGNPLPIWKCPDCGKTICVGSREELK
                     KLSGTYPEDLHKHFVDSITIPCECSGVMTRIPEVLDCWFESGAMPYAQNHYPFENKEY
                     FESHFPAAFISEGLDQTRGWFYTLTVLAAALFDKPAFKACIVNGLVLASDGKKMSKSL
                     RNYTDPNKVVNEFGADALRLFLMHSAVVKADDLKYSDEGVRDVLKGILIPLWNSYSFY
                     VTYANIDGIERPAPSSSPLASGQSANPLDRWVLSITEKMVKEVSEALDAYDLSRAIDP
                     IVAF"
BASE COUNT          412 a          432 c          429 g          440 t
ORIGIN      
        1 gggtctttta tacgaaggtc attatatcct gccctattgc ccccgctgtt caaccgtgct
       61 ttccaatcac gagcttaatc tgggtggcta caaggacgta cacgatccgg ctattaccgt
      121 taaattccgc gttacgggca caataaaggc aagcgaggcc gaacgctccg ggcttaacgc
      181 atcagattca aagccgacct tccttctggc ctggactact actccctgga ccctgccgag
      241 taatcttggc ctgacccttg gccccgacgt ggactacgtt atggtagagg actctgggga
      301 gcgctatatt ctggcagaag cccggctttc cgcttattac aaagatcccg cctccgtttc
      361 catcgtatgg aaaaaaaagg gaagcgaact tgcaggtatt acctatgagc ccctgtttcc
      421 atattttaaa gatcttgcca ccctggaaga cggccgacct ggcgcattca ggacctttac
      481 cgcggacttt gttaccaccg aggacggaac cggtatcgtg cacactgctc ccggctttgg
      541 cgaagacgac aataaggtct ttaagggaac gggagtgccg acagtctgcc ccgtagacgc
      601 agaatgccgc tttaccgcag aggttggcga ttatgcggga gtattcgtta aggatgcaga
      661 caaggctatt ctggagcggc ttaaggcaga aggccgcctc gtaaagcgtg agcaaatact
      721 tcactcctat cctcattgtt ggcgctgttc aagtcccctt atctatcggg ctgttggtag
      781 ctggtttgtc gcggtagaac agataaaaga aaaccttctt aaggctaatt ttcagataac
      841 ctggcagcct gcccatatta aagaaggacg ttttggtaaa tggcttgagg gagcgcggga
      901 ctgggcaatc agccgaaacc gtttctgggg taatcctctg ccaatctgga agtgcccgga
      961 ttgtggcaag accatttgtg tgggaagccg cgaggaactt aaaaagcttt ctggcaccta
     1021 tcccgaggac ttacataaac actttgttga ctccattaca attccttgtg aatgttcggg
     1081 agttatgact cgtatcccgg aggttcttga ttgctggttt gaatcaggag caatgcccta
     1141 tgcccaaaat cattacccct ttgaaaataa agaatacttc gaaagtcact tccctgcggc
     1201 ttttatctcc gaagggctag atcaaacgcg aggctggttt tataccttga ccgtcctggc
     1261 cgcagctctg tttgacaagc cggcttttaa agcctgtatc gtcaatgggc tcgttcttgc
     1321 ttccgacgga aagaagatgt ccaagagttt acgcaattat accgatccca ataaggtagt
     1381 aaacgaattt ggcgccgatg ccctgcgcct ctttttgatg cattcggctg tggtaaaggc
     1441 agacgatcta aaatactcgg acgaaggagt gcgcgacgta cttaagggga ttttaattcc
     1501 tttatggaac agttacagct tttacgttac ctatgcgaac atagacggta ttgagcggcc
     1561 tgctccatcc tcatctcccc ttgcttccgg tcaaagcgcg aacccgctcg atcgttgggt
     1621 gctttccatt acggaaaaaa tggtaaaaga agtaagtgag gccttggacg cgtacgacct
     1681 ttcgcgggca attgacccca tcgtcgcctt tat
//
LOCUS       DAOQXQ010000431         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_85298_length_1711_cov_4.425121, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000431 DAOQXQ010000000
VERSION     DAOQXQ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85298_length_1711_cov_4.425121"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>1711
                     /locus_tag="PKH81_07225"
     CDS             212..>1711
                     /locus_tag="PKH81_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97866.1"
                     /translation="MAAFLLCFAVLKWAVLRPEKLTPLVISNVNKAIDGKLSCKSIEL
                     TLFETFPHIGVALTDGVLVSGVTPDSVLTESGTLIAGQDSLIAFQKCIVSLRPLDYLF
                     GGKITLGEVTIQRPVIRTFIDELGKGNWEIVQTEADSATQKDTELPPIEIKRIEISDA
                     EFTFRDVRKDIFCKIEKFFFASEGSLVKGATKFDIKSKSESLLFESPQYTLTNHMRLA
                     LAGQVAFSNNYNRIALKGASLMVNNLPFDADGWVERSPDGESMQMDMEMGLTVTDLNE
                     IIPYIPDAFLKNKDQIRARGKVILEGSVRGTLDNRQYPSVALCCKIENGAYRMKDSES
                     GIDTLNLDVDLMVNGLHPDSSYLSVENSLIKGLSTSLELSGKVTDLLSNPKVDARMKG
                     SVDFTRLAKEFLNTDSLLLEGAVSSDIRLSFTQQDILSGQFGRIKTDGGLKIKNFKAY
                     SEPLGMDVFISDAVLAMGRDTASVLPKGSKGIGALQATLSMDSLQFKYKD"
BASE COUNT          392 a          363 c          502 g          454 t
ORIGIN      
        1 cttgttttct gattgcggtg caaaggtagg gtttattttt taactgcaaa actttaaggg
       61 atgttttttt aacaattttg cttacttttg ttgtcgtatt ctttattctt gtgttgaatt
      121 actaatttct tcctatacaa tgaaaactga gtggttgaaa ggaaatagca gaaaatatat
      181 tcggaccggc ttatgggtgc tgggtgtttt aatggctgct ttcctgttgt gttttgcggt
      241 tcttaaatgg gcggtgctgc gcccggagaa gctcactccc ctggttatat cgaatgtgaa
      301 caaggccata gatggtaaac tgagctgtaa aagtatcgaa cttacgttgt ttgaaacctt
      361 cccgcatatc ggggtggcgc ttacggacgg ggtgctggtt tcgggcgtta cgccggattc
      421 ggttctcacc gaaagcggga cgctgattgc cggtcaggat tcccttatcg cattccagaa
      481 gtgtatcgtt tcgctccgcc ccctcgatta cctgttcggg ggcaagatca ccctgggcga
      541 agttaccata cagcgccccg tgattcgtac ctttatagat gaactgggta aagggaactg
      601 ggagattgtg cagaccgaag ccgactcggc cacccaaaag gataccgagc tccctcccat
      661 cgagataaaa cggatcgaga tttcggatgc ggagtttacc ttccgggatg tgagaaagga
      721 tattttctgt aagattgaaa agtttttctt tgcttcggaa ggctccctgg ttaagggggc
      781 tacgaagttt gatataaagt cgaagtcgga gtcgctgctc tttgagagtc cgcagtatac
      841 gcttaccaac cacatgcgtc tggcgctggc gggacaggtg gcctttagca ataactacaa
      901 ccgcattgcg ctgaaagggg cttcgctgat ggtgaacaac ctgcccttcg atgccgatgg
      961 ctgggtagag cgctcgcccg acggcgaaag tatgcagatg gatatggaga tgggcctcac
     1021 ggttacggat ctgaacgaga ttatccccta tattcccgat gcgttcctga agaacaaaga
     1081 ccagatccgt gcacgcggaa aggtgatact ggagggtagc gtgcggggta cgctggataa
     1141 cagacagtat ccgtcggttg ccttgtgttg caagattgag aacggtgcct accggatgaa
     1201 ggacagcgag tcgggcatag acacgctgaa cctggacgtg gatttgatgg tgaacgggtt
     1261 gcatcccgat tcgtcgtacc tgtcggtgga gaacagcctg ataaaggggt tgagtacttc
     1321 gctggagctg agcggcaagg tgacggatct gcttagcaat cccaaagtgg atgcccggat
     1381 gaagggctcg gtggatttta cccgtctggc taaggagttt ctgaatacgg attcgttgct
     1441 gctggaaggg gctgtaagct cggacatccg cttgtcgttt acgcagcagg atatcttgtc
     1501 cggacagttc ggacggatta agacggacgg tgggttgaag ataaagaatt tcaaggctta
     1561 tagcgagccg ttggggatgg atgtgtttat ctccgatgcc gtgcttgcca tgggccggga
     1621 taccgcctcg gtgctgccca aaggtagtaa agggattggt gccttacagg ctacgctctc
     1681 catggatagc ttgcagttta agtataaaga t
//
LOCUS       DAOQXQ010000432         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86156_length_1701_cov_2.269745, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000432 DAOQXQ010000000
VERSION     DAOQXQ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86156_length_1701_cov_2.269745"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKH81_07230"
     CDS             <1..185
                     /locus_tag="PKH81_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNQ97867.1"
                     /translation="VMCPAFVADCIETLEEIGDRGREQFLAAGGEELHLIPCLNTHDS
                     WVKALVSLCRRVPQAL"
     gene            complement(180..>1701)
                     /locus_tag="PKH81_07235"
     CDS             complement(180..>1701)
                     /locus_tag="PKH81_07235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ97868.1"
                     /translation="DTGANIAAALIAVSSVWWLYRAGRVASSFRLPWFLGALALTLWA
                     VFDTLWALWAFLDFGNPAVHPVLQYGYLGTNLCFLGVTLYVPLANRSRRQSLQFFVDV
                     IVLFIALSAFYYVVFFWHTFLTLSHSSVSSMLYLLTDISMLSLIAAILFSYRIRSIPR
                     YLLISLCGLALFTLTDISYVWASLRGSYTPNSWIDILYALSFITHAINALLFLKAPQS
                     RFPSGSHISRSRGIIYRSLVLLLIPILAILTGKIGLAETFFFLILAFGYQTISLVLDR
                     VRQKEEALAENTMRSRILEATIADRTRELRIMNQTLENLLKRDATTGQYNRKHFTELL
                     EEWMANNTIENKIWLLILDFDRFKSVNDTYGHDVGDQVLRQIGKRMEGITNEQTFFAR
                     LGGDEFVVMLEELDSQPEIARNEALQIAEKLLAALVEPYHLAEAVLQGSGSIGIVLFR
                     DGDVPADELLRRADLSMYEAKNSGKNALCFYDDALHQAAPRTLELELNGSDRQEAL"
BASE COUNT          467 a          428 c          461 g          345 t
ORIGIN      
        1 tggtgatgtg cccggccttc gttgccgact gcatcgaaac gctggaggag atcggtgatc
       61 gagggcggga gcaattcctg gctgcaggcg gagaggagct gcacctgatt ccctgcctga
      121 acacccacga cagttgggta aaggcactgg tatcgctgtg tcgccgtgta ccacaagccc
      181 tatagggcct cttgtcgatc cgagccgttc agctccagtt ccagcgtgcg cggggcagct
      241 tggtgcaagg cgtcgtcata gaagcagagt gcgttcttgc cgctgttctt ggcttcatac
      301 atggaaaggt cggcgcgcct gagcaactcg tcggccggga cgtcgccatc ccggaacagc
      361 acgatgccga tactgccgct gccctgcagt acggcttctg ccaggtgata gggctcgacc
      421 agggcagcca gcagcttttc cgcaatctgc agcgcctcgt tccgggcgat ctccggctgg
      481 ctgtcgagtt cctccagcat caccacgaac tcatccccac ccaaacgcgc aaagaaggtc
      541 tgttcgttgg ttatcccttc cattcgtttc ccgatttgtc tgagcacctg atcgcctaca
      601 tcatggccat aggtatcgtt taccgactta aaacggtcaa aatcaaggat taagagccaa
      661 atcttgtttt cgatggtgtt attggccatc cattcttcca gaagctccgt aaaatgcttt
      721 cggttatact gaccggtagt ggcatcccgt ttaagtaaat tctccagggt ttgattcatt
      781 atcctgagct cgcgggtacg atcggcaata gtggcttcaa gaatgcgcga gcgcattgtg
      841 ttctcggcaa gggcttcttc tttttgtcgc actcgatcaa ggaccaaact gattgtttgg
      901 tacccaaagg caagaatcaa aaagaaaaag gtttccgcaa gaccaatttt cccggttaag
      961 atggccagga ttggaataag gagcaagaca agactgcgat agataattcc ccgggagcgg
     1021 ctgatatgag agcccgaagg gaaccggctt tggggtgcct taagaaacaa cagggcattt
     1081 atcgcgtgcg taataaaaga gagggcatac aggatgtcaa tccatgaatt cggcgtatag
     1141 ctaccccgaa gcgatgccca cacatagctg atatcagtaa gggtaaacag ggcaagacca
     1201 cacaatgaaa tgagtagata tcgaggtatg gatcgaatcc gataggaaaa gagaatggca
     1261 gcgatcaagc tgagcatgga aatgtcggtg agtaaataga gcatactgga aacactggag
     1321 tgcgacaggg ttagaaacgt atgccaaaaa aacacaacat aataaaacgc ggacagggct
     1381 ataaacaaaa cgataacatc aacaaaaaac tgcagggatt ggcgacggct tcgattggca
     1441 agggggacat agagggttac cccaagaaag cacagattag tgcccaaata gccatattgc
     1501 aacaccggat ggactgcagg gttaccaaaa tccagaaaag cccaaagcgc ccacagggtg
     1561 tcgaacaccg cccacagggt tagcgccagc gcgcccaaaa accagggaag gcgaaaggag
     1621 gaagcaaccc tacccgcccg atataaccac catacagagg aaacggcgat aagggcagca
     1681 gcaatattcg caccggtgtc a
//
LOCUS       DAOQXQ010000433         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86375_length_1698_cov_2.403530, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000433 DAOQXQ010000000
VERSION     DAOQXQ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86375_length_1698_cov_2.403530"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..829)
                     /locus_tag="PKH81_07240"
     CDS             complement(8..829)
                     /locus_tag="PKH81_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNQ97869.1"
                     /translation="MGRDRATVFRWIAKIKMLGILEFVRRKRECKRRRQKRKVVSEVS
                     LKIKEIRVRTGWCGQKIAKELQEKHGIKVSIPTVYRVLQGQFKLEAASKPHKHRGKTP
                     KAFGPREVIQHDTVDFGELFAHTSIDVFTKEPSIVIVDNLNSKTGVKAFKKQKIFYGK
                     AKLHQSDEGSEFKGDYPSCVKASGSSHRFSRPYKKNDQAYIENFNRSLRKECLGWIKY
                     RKEEKAEVQQMVDEYTRHFINDRWHMGLPNMMTPAQFKLWYVRNKKVTAEVAFAP"
     gene            1038..1460
                     /locus_tag="PKH81_07245"
     CDS             1038..1460
                     /locus_tag="PKH81_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNQ97870.1"
                     /translation="MTSFYWAALAAFVWGIVPIFEKAGLVRVAPMAGLFYRCAGVLLG
                     LIILILFVVKPEEIRSVDMRSAFLLVAGGFLASFIAQIFFYNALKGGSVSTVVPLSGS
                     YPLIAFILGVLLLGESFSIPKLIGMLLVVAGIWFLRLG"
     gene            1489..>1698
                     /locus_tag="PKH81_07250"
     CDS             1489..>1698
                     /locus_tag="PKH81_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ97871.1"
                     /translation="MGFPALRKISYGVYIVSSKKNDGAFNGQIANTVFQVTSEPAKIA
                     VCINTRNLTHEFISESGVFSVSVLSK"
BASE COUNT          363 a          428 c          367 g          540 t
ORIGIN      
        1 cgcaacgtta aggcgcaaat gcgacttccg cagtcacttt cttgtttcta acataccaca
       61 atttgaattg agcgggcgtc atcatgttag gcaagcccat atgccagcga tcgttaatga
      121 aatgacgggt atattcatca accatttgct ggacttccgc cttttcctcc tttcggtatt
      181 ttatccagcc caaacactcc ttccttaagc tcctgttgaa gttttcaata tatgcctgat
      241 cgttcttttt gtacggcctt gaaaagcgat gagagctgcc ggatgccttt acgcaggacg
      301 gataatcccc cttaaactct gatccttcat ccgattgatg cagtttggct ttcccataga
      361 atattttctg ttttttaaat gcttttactc cggttttgct attcaggttg tcaacaataa
      421 cgatggacgg ctccttggta aatacatcaa tgctggtatg ggcaaaaagc tctccaaaat
      481 ccactgtgtc atgctgaatt acctctctgg gaccgaaggc ttttggtgtc ttgcctcgat
      541 gtttgtgcgg ctttgaagct gcctcaagct tgaactgccc ctgcaatact ctgtagacag
      601 ttggtattga aacttttatc ccatgtttct cctgcagctc cttggctatc ttttgaccgc
      661 accaaccggt acggacgcga atttctttaa tcttcaatga aacttcgctg actacttttc
      721 tcttttgtct ccgccttttg cattctctct tgcgcctgac gaactccaaa ataccgagca
      781 tttttatctt cgctatccat cggaacaccg tagcacgatc ccggcccact ctttctccaa
      841 tcgcctccgg tttaagcccc gcctgatata gctcccaggc aagctcaaat ttgcttatgc
      901 tgttcactac aacaacccct ttctatccgt tctttcaacc cctatagaaa gtatactggt
      961 tgtcgcattt taaatgataa ttttgcgggc ccggaatgct tgcataaccc aggcaatggt
     1021 tataaaatca ttctgatatg acctcgttct attgggcagc actcgcggct tttgtatggg
     1081 gaatagtgcc gatctttgaa aaggccggac ttgtcagggt cgcgccgatg gccgggcttt
     1141 tttacagatg cgctggagtt cttctcggac tgataatcct gatactgttc gtcgtcaagc
     1201 ccgaagagat aaggtctgtc gatatgcgct ccgcttttct tttggtcgcc gggggttttt
     1261 tggcaagctt tattgcccag atatttttct acaatgccct gaaaggcggg agcgtttcga
     1321 ctgttgtccc tctttccggc agctatccgc tgatcgcttt cattcttggc gtgttgctgc
     1381 ttggcgagtc tttcagcatc cccaagctga taggcatgct tctggtggtc gctggcattt
     1441 ggttcctgcg tttgggctga ccgcttacgt gatataattc ctttacttat gggctttcct
     1501 gctttaagga agatcagcta cggggtatat atcgtttctt caaagaagaa tgacggggct
     1561 tttaatggcc agatcgccaa tacggtcttc caggtgacaa gcgagccagc gaagatcgcc
     1621 gtatgtataa acactcgtaa cttaacacat gaatttatat ccgagagcgg ggtcttctcg
     1681 gtttccgtgc tttcaaag
//
LOCUS       DAOQXQ010000434         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86440_length_1697_cov_2.685749, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000434 DAOQXQ010000000
VERSION     DAOQXQ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86440_length_1697_cov_2.685749"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKH81_07255"
     CDS             <1..856
                     /locus_tag="PKH81_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ97872.1"
                     /translation="NAQEDYTVFAADGALGATFWSFNQNPKNKDSPFYEWFTKKEFRQ
                     AMSCLVNRDRLIAQTFRGLAEPKLTLFAEANPFYNPEITLQYRYDVARAEELLASIGM
                     KRDDKGVMRDDKGRAVEFDLTISADTAQLNDAASILADDLFRVGIRLRIRATDFQKVV
                     EQLTASWDWQSVIIGLGSNYWPTQGVNVWPSTGNLHLWYPLQESPATEWEARIDYLYN
                     EGSYTLDEQKSWEIWNEYQAIILEQCPLIYLVRPRSFMAVRNRWDFTNVYFDNIGDLQ
                     IDHAFLRQ"
     gene            869..>1697
                     /locus_tag="PKH81_07260"
     CDS             869..>1697
                     /locus_tag="PKH81_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ97873.1"
                     /translation="MRFGFGDILAYVVYPFVVCKRRFPLMSFIVSRVCTMLVILFVLG
                     FAVFGLMELAPGDIVDQLMMQQLMSTESKIGQAEQMLAEEQLAASRRDLGLDRPFYEQ
                     YGMWLNRVFVHGDLGVSLISRAPVLFLIGSRLVNSMLLNLISLVLITVSSFALGVWFS
                     SKAGGAVDLAATFFALVLHAFPGILLLILLQLFASVSGLFPVTGYPSFPFSENPARFV
                     FSYAHHIFLPLLAAFLGGLGGTMRMIRATMLDQLGQPYITSLRARGISERRVYFNHAF
                     "
BASE COUNT          341 a          402 c          453 g          501 t
ORIGIN      
        1 aaatgcccag gaggactata cggtttttgc cgcagacggt gctctgggag ccacgttttg
       61 gagctttaac caaaacccta aaaataagga ttctccgttt tatgaatggt ttaccaaaaa
      121 ggaattcaga caggcgatga gctgtttggt aaaccgtgac cggctcatcg cacaaacctt
      181 tcgcggtctt gccgagccaa agcttacctt gtttgccgag gccaatcctt tttataatcc
      241 ggagatcact cttcagtacc gctatgatgt cgcgcgagcg gaggagcttt tagcttccat
      301 tggcatgaag cgggacgaca agggtgtgat gcgcgatgac aagggacggg ctgttgagtt
      361 tgatcttacc atatccgctg atacagccca gctcaacgat gcggcttcta tacttgccga
      421 cgatcttttc cgcgtaggga ttcgtcttcg cattcgcgcc acggattttc aaaaggtggt
      481 agagcagctt accgcttcct gggattggca gtcggttatt atcgggcttg gttccaatta
      541 ttggccaacc caaggcgtta acgtgtggcc gtccaccggg aacttgcatc tgtggtatcc
      601 gttgcaagag agccccgcaa cggagtggga agcgcgtatt gattacctgt ataacgaagg
      661 gagctatacc ctggatgaac aaaagtcttg ggaaatatgg aacgagtatc aggcgataat
      721 ccttgaacaa tgtccgctta tttacttggt tcgcccacgt agttttatgg cggtacggaa
      781 tcgatgggat tttactaatg tgtactttga caacattggc gacttgcaaa tcgatcacgc
      841 atttttacgg caataaaagg agcgctcaat gaggtttggc tttggggata ttctggcgta
      901 cgttgtctat ccctttgttg tgtgtaaacg ccgctttccg cttatgtcgt ttattgtctc
      961 gcgagtgtgt accatgctcg tgattttatt tgtgttgggc tttgcggtgt ttggcttaat
     1021 ggaactggct cccggagaca ttgtagacca gctgatgatg cagcaactga tgagcacgga
     1081 gagcaagata gggcaggcgg agcaaatgct tgccgaggag cagcttgccg cttcccggcg
     1141 ggaccttggt ttggaccggc ccttttatga gcaatatggt atgtggctta accgggtctt
     1201 tgttcacggc gatttgggcg taagtcttat ttcccgcgcc ccggtcttat ttttaatagg
     1261 ctcgcgcctg gtgaactcga tgttgcttaa tcttatttcg ctggttctta ttacggtttc
     1321 ctcttttgcc cttggcgtgt ggttttccag taaggccggc ggcgcagtgg atttagccgc
     1381 cacttttttt gccctggtcc ttcatgcgtt tccgggaata ctgctgttga ttttgttgca
     1441 gctttttgcc tcggtaagcg gtctctttcc ggttaccggt tatccctcgt ttccctttag
     1501 cgaaaatccc gcacgatttg tctttagcta cgcccatcat atttttctgc ccctgctagc
     1561 ggcctttctt ggcggtcttg gcggaaccat gcgtatgatt cgcgcaacaa tgctcgacca
     1621 gcttggccag ccctatatta ccagtttgcg cgctcggggc atcagcgagc ggcgggtgta
     1681 ttttaatcac gccttca
//
LOCUS       DAOQXQ010000435         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86522_length_1696_cov_2.643510, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000435 DAOQXQ010000000
VERSION     DAOQXQ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86522_length_1696_cov_2.643510"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKH81_07265"
     CDS             complement(<1..432)
                     /locus_tag="PKH81_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97874.1"
                     /translation="MAVKAMDLFEAYTQNKLPMDEGYIVSSFFKQESTYSIYEVVSYS
                     AIKDIYLTGNGLTFQTNGKKLHLLVEPPSYPQKAIEPYCRSQEFLVPLRFSESTIITA
                     KNQVKVIFSKAPQDAISAFTIVKPEGINFAFLFYSQPDVFAS"
     gene            587..>1696
                     /locus_tag="PKH81_07270"
     CDS             587..>1696
                     /locus_tag="PKH81_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNQ97875.1"
                     /translation="MITVIIDFFLEGLLSLGWQQFTMYAIGGLLIWVAIAKDYEPMLL
                     LPIGFGAILVNLPLAVVWEHEGVPGALKHLFDAGIGTELFPLLIFIGVGAMIDFRPLF
                     QRPWMMFFGAAAHLGIFITIAIAGMMGYDLKEAASIGIIGAADGPTTIYITTRFARSL
                     LGPITVVAYSYMSLVPIIQPPVIRALTTKKERLRRMEAIDHTIPASLVIVFPVAVTIL
                     ASILVPIAAPLIGFLMFGNLIRECGVLEKLSRTAQNELSNLVTLLLGITVGASMRASD
                     FIRIDTIKIMLLGMLAFIINSASGVIFAKFLNLFMKHKMNPMIGACGISAFPMSSRVI
                     QKMAMKEDPSNIILMHAVSANVSGQIGSVVAGGLVL"
BASE COUNT          399 a          358 c          406 g          533 t
ORIGIN      
        1 agaggcaaag acatccggct gtgagtagaa gaggaaggca aagttgatac cctccggttt
       61 tacaatggta aaggcagaga tagcgtcctg gggggccttg ctaaaaatta ccttaacctg
      121 attttttgca gtaatgatag tggactcgct aaaccgcagg ggcactaaaa actcctggga
      181 tcggcaatac ggttcaattg ctttttgagg gtacgagggc ggctcaacca acagatgcaa
      241 cttttttccg tttgtttgaa aggttaatcc gttgccggtt aaataaatgt ccttgatagc
      301 agaatatgag actacctcat aaatgctata ggttgattcc tgcttgaaaa aagacgacac
      361 aatataacct tcatccattg gaagcttgtt ttgtgtatac gcctcgaata aatccattgc
      421 cttaactgcc atgaactcct cctattggac ttatgtatca gtatagggcc aaaagtgaca
      481 tggcgcaatc attaaatagt ctgcaggttc ctattgtgcc tccggtagtt ttttttctgc
      541 cctgatggta ttgttggatg taagataact caaggagtgt gcactaatga taacagtgat
      601 tattgacttc tttttggaag gacttttatc gcttggttgg cagcaattta ccatgtatgc
      661 gataggcggt ttgttgattt gggtagcaat cgccaaggat tatgagccca tgttgctgtt
      721 accgatcggg tttggagcaa ttttggttaa tttgcccctg gccgttgtct gggaacacga
      781 gggggtgcct ggagccctga aacatctttt tgacgccgga attggaacag agctttttcc
      841 cctgctcatc tttattggcg tgggtgccat gattgatttt cgtcccttgt ttcagcgtcc
      901 ctggatgatg ttctttggag cggctgccca tttgggtatt tttattacga tagccattgc
      961 cggtatgatg ggctatgacc ttaaagaagc ggccagtatc ggtattatcg gcgcggcaga
     1021 tggtccgaca accatttata ttaccactcg ttttgcccgg tccctgcttg gtcctattac
     1081 cgtagttgct tattcgtata tgtctttagt gcccattatt cagccgccgg ttattcgtgc
     1141 ccttaccacc aaaaaggaac gcttgcgacg gatggaagcg attgatcata caattcctgc
     1201 ctccttggtc attgtgttcc cggtagcggt taccatactt gcaagtattc tcgttcccat
     1261 tgccgctccc ctcattggtt ttttaatgtt tggaaacctt attcgtgaat gtggagtact
     1321 ggaaaaactg tcccgtaccg cacaaaatga gttatccaat ctggttaccc tccttttggg
     1381 aattaccgtt ggcgcttcga tgagggcttc agactttatc cgcattgata caatcaagat
     1441 tatgctcttg ggaatgcttg cctttatcat taactccgcg agtggggtta tttttgccaa
     1501 attcctgaat ctttttatga agcataaaat gaatcccatg attggtgcct gtggcatttc
     1561 agcctttccc atgtcgagtc gggttatcca aaaaatggca atgaaagagg atccttctaa
     1621 tattatcctg atgcacgcgg tgagcgcgaa tgtatcagga caaattggct cggtggttgc
     1681 cggaggtctt gttttg
//
LOCUS       DAOQXQ010000436         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86584_length_1695_cov_3.736585, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000436 DAOQXQ010000000
VERSION     DAOQXQ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86584_length_1695_cov_3.736585"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /gene="mraW"
                     /locus_tag="PKH81_07275"
     CDS             <1..405
                     /gene="mraW"
                     /locus_tag="PKH81_07275"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNQ97876.1"
                     /translation="ADCVYHAVPGDYRHCRIHPATKTFQALRIAVNHELDRLPRLLDL
                     ALSALAPDGILGVITFHSLEDRIVKNFFRDASKSCICPPEVPICKCRGAPLVSLVTKK
                     AVSASESEIEMNPPSRSARLRVVRKLGTGVVS"
     gene            402..689
                     /gene="ftsL"
                     /locus_tag="PKH81_07280"
     CDS             402..689
                     /gene="ftsL"
                     /locus_tag="PKH81_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNQ97877.1"
                     /translation="MIKKLLAVLLVVAIPLCLLCTVWQSSRYVSLESELSRMEKEQYE
                     VIRVNNRLISGITILSTPERIEKVATEDLGMRKARADEILRIELKRGNIGG"
     gene            682..>1695
                     /locus_tag="PKH81_07285"
     CDS             682..>1695
                     /locus_tag="PKH81_07285"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ97878.1"
                     /translation="MADSCAPALLMTIDELVRAVEGICLCDYSQSRGFVSVVTDSRKV
                     SPGSLFIPLIGQAQDGHIYIPKALESGASVILVDRCHGEGSAQLFCTLGKNHQASFIV
                     VEDTLRALQDAARAYLKHFPNLTRIGITGSSGKTTTKEIVGSIFAVQHTVVMNEGNLN
                     SETGLPLSVFKVRKEHEIGIFEMGMNREGEIGELARVLVPSIALITNIGTAHIGILGT
                     QEAIAKQKKQIFSFFSHSDIGFVPEDSTWRDYLSSISIGTIKLFGTASLEGYKGYEDL
                     GIDGSTIFYEGLEIHFKLPGKYNLNNALGAIAIARYAGCSALEIQQGLEHVKPIFGRS
                     EIFR"
BASE COUNT          453 a          323 c          410 g          509 t
ORIGIN      
        1 gctgattgtg tttatcacgc ggttcctggt gattatcgcc attgccgcat acatcctgct
       61 accaagactt ttcaggccct acgaatcgca gttaatcatg aattggatcg cttgccccga
      121 ttacttgact tggcgctttc tgctcttgcc ccagatggaa tattgggggt gattaccttt
      181 cattcacttg aggaccggat cgttaaaaac tttttccgtg atgcaagcaa atcctgtata
      241 tgcccgcctg aagtgccgat atgtaaatgc aggggtgcgc cgctcgtatc attggtaacc
      301 aaaaaagcgg tttctgcatc ggaatctgag atagaaatga atcctccttc acgaagcgct
      361 cgtttgcgcg ttgttcggaa gcttggtact ggagttgtat catgattaaa aagttactgg
      421 ctgtactgct tgtagtggcg atccctctgt gtttgctttg tactgtatgg caaagttctc
      481 gctatgtatc tctggaaagt gagctttccc gtatggaaaa ggagcaatac gaggttatac
      541 gggtaaataa tcgcctcatt tccgggatta ctattctttc aactccagag cgtattgaaa
      601 aagttgcaac agaagatttg ggaatgagaa aggctcgagc tgatgaaata ctgcgaatag
      661 aattaaaacg ggggaatatc ggtggctgat tcttgcgctc ctgcactact gatgacaatt
      721 gatgaactcg ttagagcagt tgaaggcatt tgtctctgtg attattctca atcgagaggt
      781 tttgtttctg ttgtaacaga cagtagaaag gtttctcctg gttctctttt tattccactt
      841 attggacagg cgcaggatgg tcatatttat attccaaagg ccctggaaag tggtgcttca
      901 gttattttgg ttgatcgctg tcatggtgaa ggatcagctc agctattttg tacgcttgga
      961 aaaaatcacc aagccagttt tattgtagtg gaagataccc tgcgagcctt gcaggatgcg
     1021 gcgcgtgcgt atctaaaaca tttcccgaat cttactcgta ttggaataac tggatctagc
     1081 ggaaaaacta caactaaaga aatagttggt tcgattttcg ctgtacaaca tactgttgtg
     1141 atgaatgaag gaaaccttaa ctctgagact ggattgcccc tgtcggtgtt caaggtacgc
     1201 aaagaacatg agatcggaat ttttgagatg ggaatgaatc gcgaagggga gattggagag
     1261 cttgccaggg tgcttgttcc ttcaattgcc ctgataacaa atatcgggac tgcccatatc
     1321 ggaattctcg gcacacaaga agcaatagca aagcaaaaaa aacaaatttt ttcgtttttc
     1381 tcacattcag acataggctt tgttcccgaa gatagtactt ggcgtgatta tctaagcagt
     1441 atatcaattg gcacaattaa actctttggt actgcctcat tggaaggata taagggttac
     1501 gaggacttgg gaatagacgg ttcaacgata ttttatgaag gtcttgagat tcattttaaa
     1561 cttccgggta agtacaatct taataatgcc ttgggtgcga ttgcgattgc tcgttacgca
     1621 gggtgtagtg ctttggaaat acaacagggg cttgaacatg taaaaccaat atttggtcga
     1681 tcagagatat ttcgg
//
LOCUS       DAOQXQ010000437         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86918_length_1691_cov_3.345966, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000437 DAOQXQ010000000
VERSION     DAOQXQ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86918_length_1691_cov_3.345966"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKH81_07290"
     CDS             complement(<1..964)
                     /locus_tag="PKH81_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ97879.1"
                     /translation="MVGEQLYGFKKVGAMLEMLKSPIMKTLSPRQLMAMNYVANNSKL
                     TRVGDRVYMNTFTPYFPSLAYERYVGGILQTVQGKPTPVMMNFAVTANCICNCWHCSF
                     SDRTKKNQLSVAELKDAIGQVQDLGTSMIGFTGGEPLLRDDIEDIISLVGDRSMSLMF
                     STGWSLTRERVRSLKSAGLGIPVVSLDHYDSAKHDKRRGKEGMHAIAIKAIEMYQAEG
                     MYVAVSFVPDHELISNPEELYKTLDFFKSLGVNDMRLTSPILSGQLTEHPEEKLSKED
                     VKRVQQVQKFLVKHKGYPACFAYDVFESEKFYGCGAGFHFIFVDS"
     gene            complement(1114..1320)
                     /gene="rpmE"
                     /locus_tag="PKH81_07295"
     CDS             complement(1114..1320)
                     /gene="rpmE"
                     /locus_tag="PKH81_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNQ97880.1"
                     /translation="MKKDIHPAYNPTKITCACGNVIETCSTVKDINVEICSACHPFFT
                     GKQKLVDTAGRIDRFKKRYSIKDN"
     gene            complement(1494..>1691)
                     /locus_tag="PKH81_07300"
     CDS             complement(1494..>1691)
                     /locus_tag="PKH81_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ97881.1"
                     /translation="SIKKSGTRKEELLLTEEEMQKIWILRKVINPMDDAEIMELLLDR
                     MKKSKNNEAFLKSMNTGSAGM"
BASE COUNT          459 a          412 c          348 g          472 t
ORIGIN      
        1 aggaatcgac aaaaataaaa tgaaaaccag caccacagcc ataaaacttt tcgctttcaa
       61 atacatcata ggcaaaacat gcgggatagc ctttgtgctt aacaaggaat ttctgaactt
      121 gctgaacccg ctttacatct tcctttgata gtttttcttc tggatgttca gttagttgac
      181 cgctcaatat cggggaggta agacgcatat cgtttacccc cagagactta aagaaatcaa
      241 gagtcttata cagctcttca ggatttgaaa taagttcatg atcgggtaca aacgagacgg
      301 ctacatacat accttctgcc tgatacatct ctatggcttt tatcgcgata gcatgcattc
      361 cctctttacc ccgtcgttta tcgtgcttgg cgctgtcgta atgatcaagg ctaacaaccg
      421 gaatccccag tcctgccgac ttaagagagc gaacacgctc tcgggttagg gaccatccgg
      481 tagaaaacat caaagacata gagcgatccc ccaccagaga aataatatct tcgatgtcat
      541 cccgcaaaag gggctctcca ccggtaaaac cgatcatact cgttcccaaa tcctgtacct
      601 gaccgatggc atccttaagc tcagcgacgc ttaactggtt tttcttggtt cggtcagaaa
      661 acgaacaatg ccagcaattg caaatacagt tcgccgttac ggcaaagttc atcattaccg
      721 gcgtcggctt tccttgaaca gtttgcagga tccctcctac ataccgttca taggccaagg
      781 aagggaaata tggggtaaag gtattcatat acaccctatc ccccacccgg gttaatttac
      841 tgttatttgc aacataattc attgccataa gttgtctggg agatagtgtt ttcataatcg
      901 ggctttttag catctcgagc atagctccga cttttttaaa accgtacagt tgttcgccta
      961 ccattgaaga tcctccgttt tttagttagc acagacaatt ctagcacact caagggggaa
     1021 aacaagaacc aaattgcacg tgactccaaa agtgcgccat cgtggaaaaa aaaagccatc
     1081 ccgtttaggg atggctttat acacatataa ggattaatta tccttgatgc tgtaacgctt
     1141 cttaaagcga tcgatacgac ctgctgtgtc aacaagcttt tgcttgccgg taaagaaggg
     1201 atggcaagct gaacagattt ccacgttaat atcctttacc gtggaacagg tctcaatgac
     1261 gtttccgcac gcgcaggtaa tcttggtcgg attatacgcg ggatgaatgt ctttcttcat
     1321 ggtttcctcc aaacgaagtg tctctaataa actcgcctga atccaggctc aacggggggc
     1381 agtcgctcca tgtcacacgt gacagtaccc gtcgcagcaa cgttccccgg ggggaatacg
     1441 ccggtttctt tagaaactat atggtaatgc atggcattac cgatttccca tcatcacatt
     1501 ccggcagaac cggtattcat tgacttcagg aaggcctcat tattcttgct tttcttcatt
     1561 ctgtcaagaa ggagctccat gatctcggcg tcgtccatgg ggttaattac cttacggagt
     1621 atccagattt tttgcatctc ttcttcggtt aataaaagct cttctttacg agtaccgctt
     1681 ttcttgatgc t
//
LOCUS       DAOQXQ010000438         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_86931_length_1691_cov_2.873472, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000438 DAOQXQ010000000
VERSION     DAOQXQ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86931_length_1691_cov_2.873472"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKH81_07305"
     CDS             <1..1115
                     /locus_tag="PKH81_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ97882.1"
                     /translation="QYWKKGLYDRNYIDRSSVFRTAIKRPSDADIQSCFASMHKKTKA
                     DILNCGACGYNSCEQMAVAIYNGLNRVENCRHYMAVEVANLHEEHKREVSQAIQTVAA
                     ETAVRLQTNMQDITTLANGTTDMASCVTQSSASIEEMVANIQSINQTLQKNAETVIRL
                     EQVSTEGQGRINEIVTIVTDISEQSESLAEASSIIKQIASRTNLLAMNAAIEAAHAGT
                     VGQGFAVVADEIRKLAENAGSQASSISKALKSIKELIDNTASKTQEAQGKFDQIVDLT
                     QAVRNEEQVIKDSITEQSVGGRQVLEALTRMNDLTAQVRDETGSLLQSSKFIMDDISR
                     LSEMSSTETTVVDQAEFEAAYEVQKSDPDDEAGEIA"
     gene            1174..>1691
                     /locus_tag="PKH81_07310"
     CDS             1174..>1691
                     /locus_tag="PKH81_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ97883.1"
                     /translation="MRRIWGYVELFGVFFFLGAFTFGGGLAMLPMLERELEHKRKWIT
                     SSQLVDYYTIGQATPGIIAVNVATFVGYTRKGILGGIVATAGMVAPSLLIIMLLAAFL
                     NNYAQIVWVQKALKGINVVVASLLVQAVWRLGTKTIKDYLTALIAIASFLAIILCNAP
                     GVLVLTSSAVVG"
BASE COUNT          455 a          379 c          418 g          439 t
ORIGIN      
        1 accagtattg gaaaaagggc ttatacgaca gaaattatat agaccgctca agtgttttcc
       61 gaacggctat caagcggcct tccgacgctg acattcaatc atgttttgct tctatgcaca
      121 aaaaaaccaa ggccgacatt cttaactgtg gtgcctgcgg ctataacagc tgcgagcaga
      181 tggcggttgc catttataat ggactcaatc gtgttgaaaa ctgtcggcac tatatggccg
      241 tagaagtggc gaacctccat gaggagcaca agcgtgaggt tagccaagct attcagacgg
      301 tggccgcaga aaccgccgtt cgtctacaaa ccaatatgca ggacattacc acccttgcaa
      361 acggcactac cgatatggcc tcctgcgtaa cccaatcctc ggcatccata gaagaaatgg
      421 ttgccaatat tcagtcaatc aaccaaaccc tgcaaaagaa cgcagaaacc gttattcggt
      481 tggaacaagt ttccacggag ggtcaggggc gaattaacga gatagtcact attgttaccg
      541 atatttccga acaatcagaa tcccttgcgg aagcaagttc gataatcaag caaatagcaa
      601 gccggaccaa tcttttggcg atgaatgccg cgatagaggc cgctcatgcc ggaacggtag
      661 ggcaggggtt cgcggttgta gcggacgaga ttcgtaagtt agccgagaat gccggctccc
      721 aagcctcttc aatctccaag gcacttaaat cgataaagga actcattgat aatacagcgt
      781 ccaagaccca agaagcacag gggaaatttg accagattgt cgatttaacc caagcagtgc
      841 gcaacgaaga gcaagttata aaggattcaa ttaccgagca aagcgtcggt gggcggcagg
      901 tgcttgaagc ccttacccga atgaacgatc ttactgccca ggttcgagac gaaaccggat
      961 ccttgttgca gtcaagcaag tttattatgg acgacatctc gcggctatcc gaaatgagtt
     1021 caacagaaac tactgttgta gatcaagccg agtttgaagc ggcgtatgaa gtacagaaaa
     1081 gtgatccgga tgatgaagcc ggtgagatag cttaaagaac ttttttttct tttgataatt
     1141 ggcatccacg ggtaaaaaac ttatactgtg cccatgcgac gaatatgggg ttatgtagag
     1201 ttatttggtg tctttttttt tcttggagca tttacctttg gcggtggtct tgccatgttg
     1261 ccaatgctcg agcgcgagct tgagcacaag cgcaaatgga ttacttcttc ccaattggtc
     1321 gattattaca cgataggcca ggctacaccg ggaattatcg cggtaaatgt ggctaccttt
     1381 gtcggatata ccaggaaagg tatcttgggc ggtattgtcg ccactgcagg tatggttgct
     1441 ccttcccttt tgatcattat gctccttgcg gcatttttga ataactatgc acagatcgta
     1501 tgggttcaaa aggccttaaa gggcattaac gtggtggttg cctctctgtt ggttcaggct
     1561 gtgtggcggc ttggaacaaa gacaattaag gattacctta ctgccttaat cgcgatcgct
     1621 tcttttttgg caattattct ctgtaatgct ccgggggtcc ttgtcttaac ttcttctgcg
     1681 gtagttggct t
//
LOCUS       DAOQXQ010000439         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87177_length_1688_cov_2.881200, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000439 DAOQXQ010000000
VERSION     DAOQXQ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87177_length_1688_cov_2.881200"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKH81_07315"
     CDS             <1..751
                     /locus_tag="PKH81_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ97884.1"
                     /translation="PYAIGYGLTETSPLLAGSGPSITKVGTVGPVMPGVEMKLVDIDS
                     ATGVGEVTVRGLNVMKGYYRDEEMTRAVLDQEGWFRTGDLGTIDKTGRLALKGRSKNM
                     ILGATGENIYPEDIEFVLNQHPFVSESLVVEGENSSLVAYVQIDEEKLAKIAAEKAAQ
                     EAPQGAIQSMTEAMGEAVTGAMNDMQYAINYKREEIINEIKFFVNENVNRMSRIDRIE
                     AVHQFEKTASQKIKRYLYTISKSMSGHGGKS"
     gene            complement(808..1419)
                     /locus_tag="PKH81_07320"
     CDS             complement(808..1419)
                     /locus_tag="PKH81_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNQ97885.1"
                     /translation="MIDAVYARKWFIAHRYVVVMLAVAIFHLAALVFIRFGLGETMSE
                     PLTDYRVLKLVDIEEYAPPPPAVETTMVYNQPTASEQIVTTEDQVIEVDDPSALIAAE
                     PDYLPQHKISVIPEIPTKEILGKIRYPSLALRQQLEGVVYLELYIDQTGLVRKIQVLK
                     DPGFGFAESAVEALTGVRCKPAIANGQPVAVRFRYPVRFTLQQ"
     gene            complement(1416..>1688)
                     /locus_tag="PKH81_07325"
     CDS             complement(1416..>1688)
                     /locus_tag="PKH81_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ97886.1"
                     /translation="PEGKLLEKTQETDNLEIWIERNGNISVDGFVVTVEELEKKIAGT
                     IAENPSTRVHILADRNTPYRYVNATVTILQRLQHRVVSFVVKEELR"
BASE COUNT          432 a          376 c          457 g          423 t
ORIGIN      
        1 cccctatgcc attggctatg gattaacgga aacctctccc ttgcttgccg gttcaggtcc
       61 ttctattacc aaggttggca ccgttggacc ggtgatgccg ggagtggaga tgaagctggt
      121 ggacattgat agcgctaccg gtgttggcga ggttaccgta cgaggcttga atgtgatgaa
      181 gggctactac cgggatgagg aaatgacccg cgcagtgctc gatcaagagg gatggttccg
      241 taccggcgat ttgggaacga tagacaagac tggacggctt gcccttaagg ggcggagcaa
      301 gaacatgatt ttgggagcta ccggagagaa tatctatcct gaggatatcg agttcgttct
      361 taaccaacac ccctttgtca gtgaatccct tgttgtggaa ggagagaatt cttccttggt
      421 ggcctatgtt cagattgatg aggaaaaact tgccaagatt gccgcagaaa aagccgcgca
      481 ggaggcccct cagggcgcga tccaatccat gacggaagcg atgggagagg cggtaacggg
      541 agcgatgaat gatatgcaat acgccatcaa ttataaacgc gaggaaatta ttaacgagat
      601 aaagttcttt gtaaacgaga acgtaaaccg tatgtcccgt attgatcgaa tcgaagcagt
      661 tcatcaattt gaaaaaactg ccagtcaaaa gattaagcgg tatttgtata cgatttccaa
      721 atccatgagc ggtcacggcg gtaagtcttg atgttctgat atggtgcgga gtaaaaaaaa
      781 agcggcagga ttgtcctgcc gcttttttta ttgttggagg gtaaaacgga cgggatagcg
      841 gaagcgaacg gcgaccggct gtccgtttgc tatagccggc ttacagcgaa cgccggtaag
      901 ggcttctaca gccgattcgg caaagccgaa acccgggtcc ttaaggacct gaatcttgcg
      961 aaccagccct gtctgatcta tgtacagttc aaggtacaca acgccttcca gttgctgacg
     1021 taaggcaagg gatggatagc ggatcttgcc cagaatttcc ttggtgggaa tttccggaat
     1081 aaccgatatc ttgtgttggg gtagatagtc cggctccgcg gcgattagcg ctgacggatc
     1141 gtctacctcg ataacttgat cctcggtggt aacgatttgt tcggaagcag taggctgatt
     1201 atacaccatt gtcgtttcca cagccggagg gggaggggcg tattcttcaa tatctaccag
     1261 cttgagcacg cgataatcgg ttaagggctc actcatggtt tcgccaagtc cgaatcgtat
     1321 aaaaacgagg gctgcaagat gaaaaatagc caccgcgagc atgacaacta cataacgatg
     1381 ggcaataaac cattttctcg cgtatacggc atcgatcatc tgagttcctc cttaacgacg
     1441 aagctcacga cacggtgctg caatcgctgc aagatggtta ccgtggcgtt cacatagcga
     1501 tagggtgtgt ttctatctgc taatatatga acacgcgtcg agggattttc tgctatcgtt
     1561 cctgcgattt ttttttcaag ttcttcaaca gtaaccacaa agccatccac cgagatgttg
     1621 ccgtttcgct ctatccagat ttcaaggtta tcagtttcct gggttttttc aaggagtttg
     1681 ccctcagg
//
LOCUS       DAOQXQ010000440         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87184_length_1688_cov_2.633803, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000440 DAOQXQ010000000
VERSION     DAOQXQ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87184_length_1688_cov_2.633803"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..568
                     /locus_tag="PKH81_07330"
     CDS             53..568
                     /locus_tag="PKH81_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97887.1"
                     /translation="MLILAVILFFVNLVAFPIFYLCLKSRFSSRRVLSEIRQEMDSLI
                     VDLGRETDRDVALLESRIQGLRNLMEEAQQMILLSSRETEKRKRSSEIEHQLTMNPHK
                     EETKPVEPVRVYTRQSVIRSSSPVTIEVPLAERVLDLARKDISPQMIAKTLSVSLGEV
                     ELILDMNNSSL"
     gene            592..1407
                     /locus_tag="PKH81_07335"
     CDS             592..1407
                     /locus_tag="PKH81_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97888.1"
                     /translation="MVSTEKCYATDNSPILTASRNRGEYLYRVRVYMERARFLSPQAG
                     NTRLRVLLGFIESRTAVIDATIRHDGLLHYLSVLCSSRAPAPVFSAIMKKTAPRAIVE
                     EVSPREFSDSLTGTAMYTGSWGVIPYALPVTMVQESRNILISLLLEYRALTAELRHIG
                     DGMSVPSGTVQEGRNTSLKTSFLIFEKDSRILAIPEFQIERVSSGANGSSILYISKTA
                     GQRLFIADDLLMVRDFEVLTASIGQKVEKGIYNVHFASPDYQEALCLVIPSFL"
     gene            1484..1556
                     /locus_tag="PKH81_07340"
     tRNA            1484..1556
                     /locus_tag="PKH81_07340"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1517..1519,aa:Phe,seq:gaa)
BASE COUNT          429 a          352 c          384 g          523 t
ORIGIN      
        1 gagatcccag ccttggttca attattgata tttccggata acggagcggt atatgcttat
       61 tcttgcagtc attttgttct tcgttaatct tgttgctttt cccatttttt atctttgcct
      121 aaaatcgcgt ttctcttctc ggcgagtact ttcggaaatt cgccaggaga tggacagcct
      181 gatagtagac ttaggtcggg aaacagaccg cgatgttgcc ttactcgaaa gcagaatcca
      241 gggattacgg aatttgatgg aagaggctca acagatgatt ctcctttctt cccgggaaac
      301 agaaaaacga aagcgttctt cagagataga gcatcaactg actatgaatc ctcataagga
      361 ggaaacaaag ccggtggagc cggtgagggt gtatacccgt caatccgtaa ttcgaagttc
      421 atcacctgtt acgattgaag taccccttgc cgaacgggtg cttgatttag cccgtaagga
      481 tatttcgccc cagatgattg ccaagacttt gtcagtatcg cttggagagg ttgaacttat
      541 tttggatatg aataattcaa gtctttagcg gtactgtcga tattcgtaga gatggtatct
      601 acggaaaaat gctacgcaac agataatagt cccatcctta ccgcatcacg aaatcgggga
      661 gagtatctct atcgtgtgcg cgtatatatg gaacgagcgc gctttttgtc cccacaggcg
      721 gggaataccc gtttgcgcgt gttgttgggc tttattgagt cccgtactgc cgtcattgat
      781 gcgacaattc gccatgacgg gttattgcac tatttatctg tattgtgttc atctcgtgct
      841 cctgcgcccg ttttttcggc cataatgaaa aaaaccgctc ctcgggcgat tgtcgaggag
      901 gtatcccccc gtgaattttc cgattccctt acgggtactg cgatgtatac cggttcctgg
      961 ggtgtcattc cttatgccct gcctgtaacc atggttcagg aatcacgaaa tattctgata
     1021 tcactccttc ttgaatatag agctttaacc gccgagttac gccacatagg cgatggaatg
     1081 agtgttcctt caggaaccgt gcaagaagga cgaaatacat cccttaagac cagttttctt
     1141 atatttgaaa aggattcaag gattctcgca ataccagagt ttcaaattga acgggtatca
     1201 tcaggggcaa atggatcgtc aatactctat attagtaaaa ccgctgggca gcgacttttt
     1261 attgcagatg acttattgat ggttcgcgat tttgaggtat taactgcttc tattggtcaa
     1321 aaagtagaaa agggcatcta taatgtgcat tttgcttcac ctgattatca agaggccctt
     1381 tgtttggtta ttccttcttt cctgtaaaac agtataaatg tacgtattca atatcactaa
     1441 tcttgacttt tttttttata cacgatataa agtcatacat cgagccgttg tagctcagtt
     1501 ggtagagcaa tggactgaaa atccatgtgt cgacagttca attctgtccg acggcacaaa
     1561 gagcccctgt taggggctct ttttttttga gctttgactg ttcttgtgat atgatggcgt
     1621 aaaggggctt ttcttgaaaa ctgtcttgct atacgattat gaacatgctg acacggttaa
     1681 ggtaattc
//
LOCUS       DAOQXQ010000441         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87346_length_1686_cov_2.982833, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000441 DAOQXQ010000000
VERSION     DAOQXQ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87346_length_1686_cov_2.982833"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /gene="der"
                     /locus_tag="PKH81_07345"
     CDS             <1..1345
                     /gene="der"
                     /locus_tag="PKH81_07345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNQ97889.1"
                     /translation="PLIVIVGRPNVGKSTLFNRFLRRRRAITDPTPGVTRDPIEDVAF
                     INDKPCRIMDTGGFKLDREEGTDSAVMDSLVVEKTLQALKHADEILLVLEAGAATAED
                     EEFIALLRPYMNKLVVAVNKTEGGRREADAWNFLQYGFTEVHFVSAEHGDNITELAEA
                     ITARLDFSRVEEGAPEEIIRLAIVGKPNTGKSTLSNRLTGRQASIVSDIAGTTRDVVE
                     GDFTHKGTRFRVLDTAGIRKKSRVKEDIEYYSVNRAIKTLKHADIVIHMIDAQEGLAD
                     QDKKIIAHAANEGLGVIFVLNKWDTMDGSPKTFKDAVRNLRISFGQMEWAPVLSLSAL
                     NGTGISELLSTALRLWKQLSRRVDTSALNLALNDWITASPPPQGRMNKFKIRYLVQTQ
                     ANPVKFLAFATRPEAVSESYISYLRNRIRKDLGFDEIPVVLEVKGSRRKWEERER"
     gene            1366..>1686
                     /locus_tag="PKH81_07350"
     CDS             1366..>1686
                     /locus_tag="PKH81_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ97890.1"
                     /translation="MADYSIGEIERITGLKSHVLRYWEEALPLIRPRKDDQGRRVYGE
                     RDLELIRRLQHLVQEEKYTLEGAGERLIKELTSVPLNDAREEIRAIRDRLTDALQAVR
                     RLGTH"
BASE COUNT          427 a          439 c          469 g          351 t
ORIGIN      
        1 gccccttatc gttatcgtcg gcagacccaa cgtaggaaaa tcaacccttt ttaatcgttt
       61 cctcaggcgg cgccgcgcca ttacagatcc caccccgggc gtaacgcgcg acccgatcga
      121 agacgtagcc tttatcaacg ataagccgtg cagaattatg gacaccggcg gctttaagct
      181 ggaccgggaa gaaggcaccg actccgcggt aatggatagc ctggttgtgg aaaaaaccct
      241 tcaggcgctt aaacacgccg acgagatttt attagtgttg gaagccggag cggctactgc
      301 cgaagacgaa gagtttattg ccttgctcag gccttacatg aataagctcg tcgtagccgt
      361 taataagacc gagggcggcc gacgcgaggc agatgcctgg aactttttgc agtacggctt
      421 taccgaggtg cattttgtca gtgccgagca tggggataat atcaccgagc ttgccgaggc
      481 catcaccgca cgccttgatt tttcccgggt ggaagaagga gcgccggaag aaattattcg
      541 gctggccata gtggggaaac caaacaccgg caaaagcacc ctgtccaacc gtttaaccgg
      601 gcgccaagct tccatcgttt ccgacattgc cggcacaacc cgggacgtag tagaaggcga
      661 ctttacccat aagggaacgc gctttcgcgt tctggatacc gccggaattc gcaaaaaaag
      721 cagggttaaa gaagacatcg aatactattc cgtaaatcgc gcaatcaaga cccttaagca
      781 cgccgatatc gttatacaca tgatagatgc acaggaagga cttgccgacc aggataaaaa
      841 gataatcgcg cacgccgcca acgaggggct tggggtcatt tttgtactga acaaatggga
      901 taccatggat ggcagcccca agacctttaa ggatgcagta cgcaatctgc gcatttcctt
      961 tgggcagatg gaatgggctc cggttctatc cctgtccgcg cttaacggaa cgggaatctc
     1021 ggagcttctg tccacggcgc ttcgtctgtg gaagcaacta tctcgtcggg tggatacctc
     1081 cgccttgaac cttgcgctta acgactggat taccgcctcc ccgccgccgc agggacgtat
     1141 gaataaattc aagattcgct acctggtaca aacgcaagct aacccggtaa agtttcttgc
     1201 ctttgccacc cggcccgagg ctgtcagcga atcctacata tcctatttac gaaatcgtat
     1261 tcgcaaggat ttgggttttg acgaaatacc ggttgttctt gaagtaaagg gaagccgccg
     1321 aaaatgggag gagcgggagc gctaggtcct ggacggggag gagcaatggc agactactcg
     1381 atcggagaga tagagcgaat aaccgggctt aaaagccatg tccttcgcta ttgggaggag
     1441 gcccttccgc tgattcgtcc gcgcaaagac gatcagggcc gcagggtgta cggagagcgg
     1501 gaccttgaat tgatccgccg attacaacat ctggtacagg aagaaaaata cacattggag
     1561 ggagcaggag agcggctcat caaggagctt acctctgtcc cgttaaatga cgcgcgcgag
     1621 gagatacgag cgattcgcga tcggcttaca gacgcgcttc aggcggttcg ccgtctgggg
     1681 actcac
//
LOCUS       DAOQXQ010000442         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87558_length_1684_cov_2.647637, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000442 DAOQXQ010000000
VERSION     DAOQXQ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87558_length_1684_cov_2.647637"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PKH81_07355"
     CDS             <1..1531
                     /locus_tag="PKH81_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97891.1"
                     /translation="SAEIPSSFQRVAIPFRFARSARLEKHESLTLVRSGKKLYSFPSA
                     PLAQGDSSAVRYLPLSRSSPTVYYQTWLPAKGISITELAALPGASRQARARSVERFAA
                     SALASFRSTLLLPNLSEAVVASYVAEMGRIGMYRTAIESVPASFTSGQSRTWLTNTFF
                     NSLERTWAGMISREREERVALSRKLTEGNPAIFEFPSLFTYLIDRGSAVLLPDVVRVA
                     SALEIASISPVQAAGILETILDARDMQSPHAVALEALSESAERRIISSFALVQDRLYM
                     SKNGSLVDSLDTLRVASVLSRWGASDPKKEQWAGMSNLLVTSLLTHLGEDGSLPVSLA
                     LSPETGERRESIAVSEGGFIDPGRLYPHVVTGDTWYPHALSLSSVLGPGSWAWTSAKS
                     VTVAKRSGDVLAITVGFPQGETHYMVIRGIKPFYRIQLYGMDFRTDPRFESYNSSGYR
                     YNEDTETLFLKMRHKVEMEDIVIYLGAPAPVAPVAEEGSNASVEGAGEDAAPPVQPSG
                     L"
BASE COUNT          331 a          458 c          445 g          450 t
ORIGIN      
        1 ctccgcagaa attccctcgt cctttcagag ggtagccatt cccttccggt ttgcacgcag
       61 tgcccgcctg gaaaagcatg aatcccttac gttggttcgt tccggaaaga agctgtactc
      121 ctttccctct gctccgctcg cgcagggaga tagctctgct gttcgctatc ttccgctctc
      181 tcgttcgtcg ccgacagtgt actatcaaac atggcttcct gcaaagggta tctctattac
      241 tgaactcgcc gccctgccag gggcctctcg tcaggcacgc gctcgctctg tcgaacgctt
      301 tgccgcttcc gccctagctt cgttccggtc tacgctgctt cttcccaatc tttccgaagc
      361 ggttgtcgct tcctatgtag cggaaatggg ccgaatcggg atgtatcgca ccgcgattga
      421 aagcgtccct gccagcttta cctccggcca atcgcgtacc tggcttacca ataccttttt
      481 taacagtttg gagcgtacct gggctggtat gatctctcgt gagcgcgagg agcgtgtggc
      541 gctttcccgt aaactaaccg aggggaatcc agccatcttt gaatttccct ctctttttac
      601 ctatttaata gaccggggga gtgcggtgtt gttgcctgat gtggtgcgcg ttgcctctgc
      661 cttggaaatc gcatccatta gtcctgtgca ggctgcagga atcctggaga caattttgga
      721 tgcgcgggat atgcaaagcc cgcatgcggt tgcgctggag gctttgagtg aatctgccga
      781 acggcgaatt atttcttcgt tcgccctggt tcaagatcgc ctctatatgt ccaaaaatgg
      841 gtccctggta gattccctgg ataccctgcg agtggcctct gtcttatccc gttggggcgc
      901 atctgatccc aagaaggaac agtgggctgg tatgtccaac ctccttgtta catcgctgtt
      961 aacccatctt ggcgaggatg gatcccttcc ggtctccctg gccctaagcc cggaaaccgg
     1021 cgaacgtcgt gaatccatag ctgttagcga aggtggattt atcgatccgg gtcgcttgta
     1081 ccctcatgta gtaaccggag acacctggta tccgcatgca ctttcgctgt cttcggtttt
     1141 ggggcctgga agctgggcat ggaccagcgc aaagtccgtt acggtcgcca agcgaagcgg
     1201 ggatgtgctt gccataacag ttggattccc ccagggcgaa actcattata tggttattcg
     1261 aggcattaag cctttttatc gcattcagct gtatggtatg gattttcgca cagatcctcg
     1321 atttgaaagt tataattcat cgggataccg atataacgag gatacagaaa ccctgttctt
     1381 aaagatgcgg cacaaggtgg agatggaaga tatcgtgatt tatctaggcg ctccggctcc
     1441 tgttgcgcct gtagcggaag aaggatctaa cgcttcggta gaaggcgctg gcgaagatgc
     1501 agctcctccg gtacaacctt cgggattata atggatcagg cctgtccggc ctgttccatt
     1561 atctggtcaa actcttcatc ggtatcgcaa acaagggctg cggcaaagta gagagctatc
     1621 gctgtttcgg tggtaagcgt tcccatgggg acagccccct ccctccacat tcgccttgag
     1681 gttg
//
LOCUS       DAOQXQ010000443         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87670_length_1683_cov_2.050983, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000443 DAOQXQ010000000
VERSION     DAOQXQ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87670_length_1683_cov_2.050983"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKH81_07360"
     CDS             complement(<1..680)
                     /locus_tag="PKH81_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97892.1"
                     /translation="MGRNTNSIDVRHSDDTVNTILSSTQLVPTKGEHPVRLYYSQSED
                     QFVLLDAPLSVPALPIHHPMDNTEPPPGYCASLATLVSAIMEHYPSLLAGTRWFFNPV
                     SIHNPGFYRIERFQDQDYLYLCFLDLSCRPLESEITEQGSNDRTHAYRSRRLYFECDY
                     LPLSAEGIQDGKVHLAHTIPITWKGESGEGYMVHGIWMDSDINKFFSKMILPTGKRNH
                     PYYPVTCK"
     gene            729..1664
                     /locus_tag="PKH81_07365"
     CDS             729..1664
                     /locus_tag="PKH81_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNQ97893.1"
                     /translation="MLILLLAPSFLWASPYGIIAYAEGGSFARIRDGVTEVFNSSDGA
                     VFGMEVRPGDMYQTRSGCFLELAIYPGAASVQIAENTSFKCDQDISASKPGGELYYGR
                     VRAKVVKLSGKAPYRISSPSLVAGVRGTEFGLDVIAARGERNLHRVFCLEGSVLVGDL
                     SGSILNTVIIGKNEMVERLADSGALQEQSPQLEKIPVRAEVLDFWQGHEYSGVDPILI
                     ALDTPISYTEGSLTITRRVWPKGGESFFYRQNLPLVRGMSSLLITVGTISCVAASGWY
                     TEKDPDARFIDPAYSAGLIMIGSGTVLTLLSLFAD"
BASE COUNT          368 a          390 c          471 g          454 t
ORIGIN      
        1 tgtttgcatg ttaccggata atacggatga tttcgcttgc cggtgggcaa gatcatcttg
       61 gaaaaaaact tgtttatatc actgtccatc catattccgt ggaccatgta tccttctccg
      121 gattcaccct tccaggttat cgggatggtg tgggcaagat gcacttttcc gtcttgaata
      181 ccctctgcgc taaggggcag atagtcgcat tcaaagtaga gtcgccgtga tctataggcg
      241 tgggtcctgt cgttgcttcc ctgttcggtt atctcgcttt caagcggtct acagctcagg
      301 tccaaaaaac aaaggtacag gtaatcctga tcttgaaagc gctctatgcg gtaaaatccg
      361 ggattgtgaa tgctgaccgg attaaaaaac cagcgggtgc cggcaagcag gctggggtag
      421 tgctccataa ttgccgaaac aagggtcgca agcgaggcgc aatacccggg cggcggttcg
      481 gtattgtcca tgggatgatg tattggcagg gcgggtacac tgaggggcgc gtcaagcaag
      541 acaaactggt cttcgctttg actataatag aggcggacgg ggtgctctcc ctttgttggg
      601 acaagctgag tcgatgagag tatggtattt acggtgtcgt ccgaatggcg tacatctatc
      661 gaatttgtgt tccggcccat tggacattat actaacgtat aatgaaccga aataatagat
      721 atatcggtat gcttatcctt ctcctggcgc catcctttct gtgggcttct ccgtatggca
      781 ttatcgcgta cgccgagggt ggttcatttg cgcgcatcag ggatggggtt acggaggttt
      841 ttaactcgag cgacggtgct gtctttggta tggaagtgcg gccgggtgac atgtatcaaa
      901 cccgttctgg ctgttttttg gaactggcga tttatcccgg agcagcatcg gttcagattg
      961 cggaaaacac ctcgtttaag tgcgatcagg acataagcgc atctaaaccg ggaggcgagc
     1021 tctattacgg gcgggtgcgg gcaaaggtag tcaagttaag tggaaaagcc ccgtatcgca
     1081 tttcctctcc gtccttggtc gcaggggtcc gtggtacgga atttggtctt gatgtaattg
     1141 ccgctcgggg cgagcggaat ctccatcgtg tgttttgtct tgagggaagc gtgttggtcg
     1201 gagacctttc cggttctatt cttaatacgg ttataattgg aaagaacgaa atggtagaac
     1261 gccttgccga ctctggcgct ttgcaggaac aatccccgca actggaaaaa attcccgtac
     1321 gtgccgaggt tcttgatttt tggcagggtc atgagtatag cggggtagac ccgatcctta
     1381 ttgcccttga taccccaata tcgtataccg aaggctctct taccataacc aggcgggtgt
     1441 ggccaaaagg cggcgagagc tttttttatc gccaaaatct cccgctcgtt cgaggaatgt
     1501 cgtccttgtt gataacggta ggaaccatct cctgtgtcgc ggcctctggc tggtacacag
     1561 aaaaggatcc ggatgcccgc tttatcgatc ccgcctattc tgccgggctc attatgatcg
     1621 gctccgggac ggtattaacc ctgttaagct tgttcgccga ctagctatcg ctcaggatac
     1681 act
//
LOCUS       DAOQXQ010000444         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87728_length_1682_cov_2.524892, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000444 DAOQXQ010000000
VERSION     DAOQXQ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87728_length_1682_cov_2.524892"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PKH81_07370"
     CDS             complement(<1..1318)
                     /locus_tag="PKH81_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNQ97894.1"
                     /translation="MKKRTHFLLLSVLYLCLLSLVYADPVENPAALSGVLDLRTHSFK
                     QSPSRSLEGEWLFYPQQLLSPTDLEDPALPAPTVQHLPGYWSSSAMPAHSFATYAVTV
                     MLPREVVDNAVVLGLRISDSISACSVWIDNDSVFSSGVVGRTKQDEQPQFKPGNVYFT
                     PRSASVRILIQVSNHNYRVGGIWAIPRISIASVAASEWSGSTGLDLFLLGALLIMVVN
                     QSALFISRRKERSALWFACLCLFIALRIVVTGNCLLTQWIPTFSWEIARKFEFLPICL
                     GPVFFALFIHELFPDCMKSPLRKGVYFVGLFFALLVLALPAVFSSYLVIPMEIYLALV
                     IVWLVTILCRARIQRLDGSGWLLVGYASLALTVINDILFSQLLIRTVYLLSFGFFCFI
                     GSQVILLARTLTHGFNRSEEIALELETTNKSFGRFVPTQFLKLLNRD"
     gene            complement(1415..>1682)
                     /locus_tag="PKH81_07375"
     CDS             complement(1415..>1682)
                     /locus_tag="PKH81_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ97895.1"
                     /translation="RFTERPAVRAVFYGLRAGTTGMIAVAGWQVLSIAVCTIPRFKET
                     CKVLDLFSWPAVCLFVVSLVTGIRTQWHPLVFVTVGALFGIFFL"
BASE COUNT          559 a          389 c          405 g          329 t
ORIGIN      
        1 ggtcacgatt taaaagcttg agaaactggg ttggaacaaa tcggccaaag gacttgttgg
       61 ttgtttccag ctcaagggca atctcttcac tccggttaaa gccatgggta agggttcgtg
      121 ccaggaggat aacctgggaa ccaataaaac agaaaaaacc gaatgataaa agatatacag
      181 tcctgatgag caactgcgaa aaaaggatgt cgttaattac ggtgagtgca agagaggcat
      241 atccgacaag gagccagccg ctaccatcaa gccgttggat cctggcgcgg cacaagatgg
      301 tgaccaacca tacgataacc agggccaggt atatctccat gggaatgacc aggtaacttg
      361 aaaaaacagc gggtaatgca agtaccagaa gtgcaaaaaa caaaccgacg aaatatacgc
      421 ccttacgaag cgggcttttc atacaatcag gaaaaagctc gtggataaac agggcaaaaa
      481 aaacaggccc aagacaaata ggaaggaatt cgaattttct ggctatctcc caactaaacg
      541 ttggtatcca ctgggtaagc aggcaattgc ccgtaacaac aatcctgaga gcaataaaga
      601 ggcacaaaca ggcaaaccac agggcagagc gctctttacg tcgtgaaata aacagggcgc
      661 tctggtttac caccataata agcaaggccc ccaataaaaa caaatccagt cccgtagatc
      721 cggaccactc ggaagcagcc accgacgcaa tggaaatgcg gggaatggcc caaattccgc
      781 caacgcgata attatgattc gatacctgaa taagaatgcg taccgatgct gagcgagggg
      841 taaaatacac gtttcccggc ttgaactgag gttgttcatc ctgctttgtt cgaccaacaa
      901 cgccactgga aaatacagag tcattatcaa tccacaccga acaagcgctt attgaatcgg
      961 atatacgcaa accaagaaca acagcattat ccaccacctc gcgggggagc ataacggtta
     1021 ctgcataggt tgcgaaggag tgggcaggca tggcagaaga cgaccagtat ccgggaagat
     1081 gttgcactgt aggagcaggt agggcaggat cttccaggtc agtcgggctg agtaattgct
     1141 gcggataaaa aagccattca ccttccaaag agcgtgatgg gctttgctta aatgaatgag
     1201 tccgcagatc aagaactccg gacaaagcgg cagggttttc aacaggatcg gcatacacaa
     1261 gcgacaacag gcatagatat agtacagaga gaagtaaaaa atgtgttcgt tttttcatgt
     1321 tttttccata ccgagtgtgt taattgtaaa ctaacggtac agagattacc acagaccttt
     1381 tttaggagac atatcttttt acattgaaac gacactagag aaaaaaaatc ccgaatagcg
     1441 ccccaacagt tacaaatacc agtggatgcc actgagttcg tatccccgta accaacgata
     1501 cgacaaaaag acataccgca ggccaagaaa aaagatccag gaccttgcag gtttccttaa
     1561 agcgaggaat agtgcatacc gcaatggata gaacctgcca tcccgccact gcgatcatgc
     1621 cggttgtacc ggcgcgcagt ccataaaaga ccgctcgaac cgcaggacgc tcggtaaaac
     1681 gc
//
LOCUS       DAOQXQ010000445         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87909_length_1680_cov_2.611077, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000445 DAOQXQ010000000
VERSION     DAOQXQ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87909_length_1680_cov_2.611077"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="rplJ"
                     /locus_tag="PKH81_07380"
     CDS             complement(<1..307)
                     /gene="rplJ"
                     /locus_tag="PKH81_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNQ97896.1"
                     /translation="MAIQAKKLQPAKTAAIADIKTRFETVTDYIFTEYRGLSVEQISA
                     LRDQLREKGCTYKVVKNNFARIAFEELNKGDVSSFFVGPTAVALPTEEANTAAKVLFD
                     "
     gene            complement(310..990)
                     /gene="rplA"
                     /locus_tag="PKH81_07385"
     CDS             complement(310..990)
                     /gene="rplA"
                     /locus_tag="PKH81_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNQ97897.1"
                     /translation="MKHGKNYRNALKKFDLLQQYDLPKAIEMIKELKFAKFDETVEIH
                     VALKLGKSQTVRDTVVLPNQFRGEKKVIVFCKEDRVKEALDAGAAFAGADEYIEKVKG
                     GWMEFDIAVATPDMMKDVGRLGMVLGRKGLMPNPKTGTVTNDIAAAINELKKGRVEFR
                     ADKTGVIHLPVGKVSMEATKIVENIEAFLTEMARKKPSDAKADFVQSVSVSSTMGPGV
                     WVAYKAGE"
     gene            complement(992..1423)
                     /gene="rplK"
                     /locus_tag="PKH81_07390"
     CDS             complement(992..1423)
                     /gene="rplK"
                     /locus_tag="PKH81_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ97898.1"
                     /translation="MAKKKITALIKLQCPAGKATPAPPVGPALGPHGVSAPQFVQQFN
                     DRTKTMEPGLIIPVVITVYQDKTFTFILKTPPASVLIKKACNLPKGSENSATHKVAVL
                     SKAKLEEIAKIKMPDITANDIEAAKKIVAGTARSMGVEVER"
BASE COUNT          428 a          417 c          390 g          445 t
ORIGIN      
        1 agtcgaaaag cacctttgcc gcggtgtttg cttcctcggt aggaagggca actgcggtcg
       61 gtccaacaaa gaaggaagaa acatcaccct tattcaattc ctcaaaagcg atgcgcgcaa
      121 agttgttttt aacaaccttg taggtgcaac ccttttcccg aagctgatcg cgaagagcag
      181 aaatctgttc tacggacaaa ccgcggtatt cggtaaagat atagtcagtg acggtttcaa
      241 acctggtctt gatgtcggca atagccgcgg ttttagcggg ctgtagtttc tttgcttgta
      301 ttgccataat tactctcctg ccttataggc aacccagaca ccggggccca tggtggaact
      361 gacggaaaca gactgaacga agtcggcctt agcgtcagaa ggcttcttac gcgccatttc
      421 ggtaaggaag gcctcaatat tttccacgat cttggtggct tccatggaaa cctttccgac
      481 cggaagatgg ataacaccgg ttttatcggc gcggaactca acacgaccct ttttaagctc
      541 gttgatagcc gctgcgatat cgttggttac cgtaccagtc ttgggattgg gcataagacc
      601 cttgcgtcca agaaccatac cgagacgacc tacgtccttc atcatatcgg gggtagcaac
      661 ggcaatgtcg aattccatcc aaccgccctt taccttctcg atatattcat cagcgcctgc
      721 aaaggcagca ccggcgtcaa gggcttcctt aacacggtct tccttacaga acacgattac
      781 ctttttttct cccctaaatt gattgggaag aacaacggtg tcgcgaacag tctgactctt
      841 gccgagcttg agcgcgacat gaatttctac ggtttcgtcg aacttggcga acttaagttc
      901 cttgatcatc tcaatagctt tgggaagatc gtattgttga agtaaatcaa acttcttgag
      961 cgcattgcga tagttttttc cgtgtttcat attatcgctc cacctctaca cccatactgc
     1021 gagcagtacc ggcgacaatc ttctttgccg cttcgatatc gttagcggta atgtcgggca
     1081 tcttgatctt ggcgatttct tccagctttg ccttggaaag aacagcaacc ttgtgagtcg
     1141 cagagttctc tgatcccttg ggaagattac aagccttctt gataaggaca gaagcaggcg
     1201 gagttttgag gataaacgta aaagtcttat cctgatacac cgtgattaca accggaataa
     1261 taagtccagg ttccatggtt tttgtgcgat cattgaactg ctgcacaaac tgaggagcac
     1321 taacgccatg aggaccaagc gcggggccga cgggaggagc aggggttgct tttcccgcgg
     1381 gacactgcag cttgatcagg gcagtgatct ttttcttagc cataaggttc tcctatttgg
     1441 tgatagcgac gcttcggagt aacacaccga gttttacagg aactaaagtc ctgcaaacga
     1501 aagaaagcca acaagcttct ttccgtctcc catatatgaa agaacgaaaa gcgtcctgaa
     1561 acaggacgcc ctcttcttgc atgctatatt ttttctacct gtaaaagatc aacttcaacc
     1621 ggtgttgcgc gaccaaagat tcataccata acacgcaact tattgcgctc ggtattaacc
//
LOCUS       DAOQXQ010000446         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87918_length_1680_cov_2.436308, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000446 DAOQXQ010000000
VERSION     DAOQXQ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87918_length_1680_cov_2.436308"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKH81_07395"
     CDS             <1..489
                     /locus_tag="PKH81_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97899.1"
                     /translation="DGEGFALPREPIRSAMGLAFNAERNNPTPEASILIPITTNVKTF
                     LDKQLIDIDIHPLIPASRIEVSVFADRGTAVYSANSPFELSEGGRRSLFVSPDNPDYP
                     YTLRFSTDRQTILTATIRILTAENPWQLEPVSPGFIGRYVLDCSRTVPVGLNGAQQRD
                     GE"
     gene            492..>1680
                     /gene="dinB"
                     /locus_tag="PKH81_07400"
     CDS             492..>1680
                     /gene="dinB"
                     /locus_tag="PKH81_07400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNQ97900.1"
                     /translation="MSAQELFFHVDLDAFFASVEQLDNQALRGKPVIVGGDPSKRGVV
                     STCSYEARTFGVHSAMPMAQALRLCPQAIVVQGRMRRYHDKSREVMHIFSSFSPDVQQ
                     ISVDEAFLDMSGTERLFGNAEKSARELKKRVTEETGLTVSVGAAPNRYVAKIASALSK
                     PNGLVIVQPGEEEAFMLSLRLKDVWGVGEKSRERLIKAGLSTVTLIHDTPEPLLRALF
                     GDALSAFLFQAVRGIDPGILSGETANRSISAEHTFAHDIRDRNIVETLLLELSQELSF
                     RLLDEDLSARTVTVKIRYDDFRTVSIQETLKQDIIDSAELFERARLLFRKKDEREKEI
                     RLIGLGLSHVQDRGATEQLDLFPDKAQKKREQVEKALHALTKKRGKRMVTRARLIGGD
                     RREQ"
BASE COUNT          416 a          418 c          421 g          425 t
ORIGIN      
        1 gatggagaag gctttgccct gccccgagag ccaattcgtt cggcaatggg gctagccttt
       61 aatgctgagc gaaataaccc gacgcctgag gcgtccatcc ttattccaat aaccaccaat
      121 gtaaaaacct ttttggataa acagcttatc gatatagaca tacacccact catacccgca
      181 tcccgcatcg aggtgtccgt ttttgccgat aggggaacgg ctgtgtactc agctaacagc
      241 ccttttgaac tgagcgaagg cggccgcagg tcattatttg tttcccccga taatcccgac
      301 tatccctata cccttcgctt ttccacggat cgccaaacca tacttactgc cacaattcgc
      361 atcttgactg cggaaaatcc ctggcagctt gaaccagtct ctcccggctt tatcggtcgt
      421 tatgtgcttg actgctctcg caccgtccct gtgggtctga acggcgcgca acagagggat
      481 ggcgaatagc gatgagtgct caagagcttt tttttcatgt tgatcttgat gctttttttg
      541 cgtctgttga gcaacttgac aatcaggcgt tgcggggcaa acctgttatt gttggaggag
      601 acccgtcaaa gcgaggggta gtctcaacct gctcgtacga ggcccgaact tttggcgttc
      661 attcggcaat gcccatggcg caagccctta ggctctgtcc acaagcaatc gtcgtccagg
      721 gccgaatgcg ccgatatcat gataagtcgc gagaagtgat gcacattttt tcctcgtttt
      781 ctcccgatgt ccaacagatt tccgttgatg aggcctttct ggatatgagc ggaaccgaac
      841 ggcttttcgg gaacgcggag aaaagcgcgc gggaattaaa aaaaagggta accgaagaaa
      901 ccggtcttac agtgtctgtc ggggccgcac caaaccggta cgttgccaag atagcctctg
      961 ccctttccaa gccaaatggt ttggtaattg ttcaaccagg agaggaagaa gcgtttatgc
     1021 tttcccttcg tcttaaggat gtgtggggtg tgggagagaa atccagggaa agacttataa
     1081 aagcaggact atcgactgta accctgattc acgacacacc cgaaccgcta ttgcgtgccc
     1141 tttttgggga tgcactttct gcatttctct ttcaagcagt tcgtggtatt gatccgggga
     1201 ttctatccgg tgaaacggca aacaggtcta taagcgcgga gcacaccttt gcacacgaca
     1261 tccgggaccg gaacattgtg gagacccttc ttcttgaact ttctcaggaa ctttcatttc
     1321 gtcttttaga cgaggactta tccgcacgca ccgttacagt taaaattcga tatgatgatt
     1381 ttcgcactgt cagtatccag gaaaccctta agcaagatat tatcgattct gccgagcttt
     1441 ttgagcgggc gcgcctcttg tttcgaaaaa aggatgagcg cgaaaaggaa attcgcttaa
     1501 tcggtcttgg cctttctcat gtacaggatc gaggtgcaac tgagcaattg gatctgtttc
     1561 ccgataaggc ccaaaagaaa cgagaacaag ttgaaaaggc tcttcacgcg ctgacaaaaa
     1621 agcgggggaa gcgaatggta acaagagctc gccttatagg cggggataga cgcgaacagt
//
LOCUS       DAOQXQ010000447         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87967_length_1679_cov_3.334975, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000447 DAOQXQ010000000
VERSION     DAOQXQ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87967_length_1679_cov_3.334975"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKH81_07405"
     CDS             complement(<1..885)
                     /locus_tag="PKH81_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ97901.1"
                     /translation="MENVTFLPGKPLPLGATVTSDGVNFSVFSRHASSVILEIFRSAS
                     DAEPAVSYTLNRNHNRTGDVWHVLARGLRPGALYLYRVDGPFRPQEGLRFNVHKYLVD
                     PYAKALSGESIFKGLDKASKRPPHMDADLAYSSTCSAEGFPKCVTVDDDAFDWEGDRP
                     LNYPLRHSIIYEAHVKGLTRHESSSLGHSGTYLGVIEAIPYLKELGITSLELLPIQEF
                     DEHEIPRVNPRTGERLSNYWGYSTISFFAPKAGYASDGTPGAAVREFKEMVKALHRSG
                     IEVILDIVFNHTAEGNERG"
     gene            948..>1679
                     /locus_tag="PKH81_07410"
     CDS             948..>1679
                     /locus_tag="PKH81_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HNQ97902.1"
                     /translation="MKSIPIVSKKKTTVVAFRDACIRSDGDFIPIILSKEEEAISYFN
                     YELPEIKIIDFGDESINAPNIMKVIHEDPWLLFGGIIAVVSNHQQKTELEKQRDPNIL
                     LVLRRPDFEANADRILKILTQNKQFLFNRGMQQQMNTNETGSFTSDNDPMDILFYCNL
                     ITSYLYNTNRIDDEGRSGVQGALMELLLNAVEHGNCKISYDEKTEWLAAGKNMLDLIS
                     AKSKEPDAINKKVYIAYDISNMRTRI"
BASE COUNT          495 a          422 c          393 g          369 t
ORIGIN      
        1 cccgcgttca ttaccttcgg cagtgtggtt aaaaacaata tccaatatta cttcaatacc
       61 cgagcgatgc agagccttta ccatctcctt aaattcacgg accgcagcgc caggagtccc
      121 gtcggaggca tagccagcct tgggggcaaa aaaggatata gtgctgtagc cccagtaatt
      181 ggacagtcgc tctccggttc tggggtttac ccggggaatc tcatgctcgt cgaattcctg
      241 aatcggtaaa agttcaaggc tggtaatccc cagttccttc aggtacggaa ttgcctcaat
      301 aactcccaga taggtaccac tatgaccaag ggatgatgat tcatggcggg taaggccctt
      361 aacatgcgcc tcgtaaataa tactatgccg caggggataa ttaagcggac gatcaccttc
      421 ccaatcaaag gcatcatcgt cgacggtcac gcattttgga aaaccttcag ctgaacaggt
      481 gctggaatag gccaagtccg cgtccatatg gggcggccgc ttggaggcct tatcaaggcc
      541 cttaaaaatt gattctcccg agagtgcctt tgcataggga tcaacaagat atttgtgcac
      601 attaaagcga aggccttcct gaggccgaaa gggtccgtcc accctataca gatacagggc
      661 tcccggccgt aagccccgag caagaacatg ccacacgtct ccggtacgat tgtgattgcg
      721 attaagggta taacttaccg ccggctccgc gtcggaagcg gagcgaaaaa tttcaaggat
      781 gacggacgag gcatggcgag aaaagacgct gaaatttacc ccgtcagagg taacagtcgc
      841 gcccagcgga aggggttttc ccggtaaaaa ggtcacattt tccatacaga ttccgaaagt
      901 atagtccgtt gggggatttt atgctagaat aacaggatgg agcgcgtatg aaaagtatcc
      961 ccatcgtcag caagaaaaaa acaaccgtag tagccttccg ggatgcctgt atcagatcag
     1021 acggagattt tattcccatc atcttgagca aagaagaaga agctatttcc tatttcaatt
     1081 acgaattacc cgaaataaag attattgatt ttggggacga aagcatcaac gcacccaata
     1141 tcatgaaggt aattcacgag gacccctggc tcctttttgg cggtataatc gcagtggtga
     1201 gcaatcacca acaaaaaact gaactggaaa aacagcggga ccccaacatt cttctggtgt
     1261 tacgcagacc cgattttgaa gctaacgccg accgcatcct caagattctg acccaaaaca
     1321 agcagtttct ctttaaccgc ggaatgcagc aacagatgaa taccaacgaa acaggatcgt
     1381 ttaccagcga taacgacccc atggatatcc ttttttactg caatcttatt accagttacc
     1441 tatacaatac aaaccgcatc gacgatgaag gccgctccgg ggttcaggga gcccttatgg
     1501 agcttttgct caacgccgtt gaacacggaa actgcaagat aagctacgac gaaaaaaccg
     1561 aatggcttgc cgcgggcaaa aacatgcttg acttgatctc ggcaaaaagc aaggaacctg
     1621 acgccatcaa caaaaaggta tatatcgcct acgacatatc caacatgaga acccgcatc
//
LOCUS       DAOQXQ010000448         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_87978_length_1679_cov_2.805419, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000448 DAOQXQ010000000
VERSION     DAOQXQ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87978_length_1679_cov_2.805419"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1679
                     /locus_tag="PKH81_07415"
     CDS             155..>1679
                     /locus_tag="PKH81_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ97903.1"
                     /translation="MEQLATIPLVFRERVRKNPEVMVQAYKIEGGCYGTRTYRQLYED
                     VLDFACALQTLGVSRSQHIGLISDNRREWLISDLAIQSLGSADVPRGCDSMEKEIIFI
                     LSTTDCPLVIVENKNQLTKVLNGKDSLPHLKRIIMFNEPTEEDRKDAKKKGIELFSFW
                     TVLGDGTAKRAGDATVIEAEIDKGTKEDLATIIFTSGTTGEPKGVMLTQGNYIWQLER
                     TPHLLYGEPGDIWLTVLPVWHSFERLMQYIVIERSSGMVYSKPVASVMFPDIAAMKPH
                     VIPGVPRLWDALANGILRTVRKEGGLKKALFFFFLSVGKKYCTAHDLVFGRVARFTRR
                     VRALDTLVGLIPWLLLLFPRFLGNVLVYRKIRQKLGGRIRFCISGGGALQKDVDAFYR
                     AIGLKVIEGYGITETAPVLSLRDQWKPRPGCVGAVFTDTECRIVDSDALDSAISSAKD
                     GLWRVPPALPPGKQGVIVVRGGQVMKGYYKRDDLTKKAIDADGWFNTGDLGMLSWDNE
                     "
BASE COUNT          429 a          362 c          447 g          441 t
ORIGIN      
        1 gccttccccg aacgaggtga gggataaaac aagggcagaa caattccatg aacggttggc
       61 cattgcccta taatgagtaa atggaggctt cctgtcaata aaacttgaca acctgacgta
      121 aaccgtattt atgtaagtaa ggaggttcaa caatatggaa caattagcca caattcccct
      181 ggtttttagg gagcgggtaa gaaaaaaccc ggaggtcatg gttcaagcat acaagattga
      241 ggggggatgc tatgggacgc gaacataccg gcaattgtat gaggatgtgt tggactttgc
      301 ctgtgcacta cagacacttg gtgtttcccg atcgcagcat atcggcttaa tttcggataa
      361 ccgccgagaa tggcttattt cggatctggc gattcaatcc ctgggctccg ccgatgtgcc
      421 gcgcggatgc gactcgatgg aaaaagagat tatttttatc ctgtccacca ctgattgccc
      481 actcgttata gtagaaaaca aaaatcagtt aactaaggta cttaacggca aagatagctt
      541 gcctcatctt aagcggatta tcatgttcaa tgagccgacc gaagaagacc gcaaagatgc
      601 caaaaaaaag ggtattgagc tgttctcctt ttggactgtt cttggggatg gcacggcaaa
      661 acgtgccgga gacgctaccg tcattgaagc ggagatagac aagggaacaa aagaggatct
      721 ggcgaccatt atctttactt ccgggacaac cggagagccc aagggcgtta tgctcaccca
      781 gggtaattat atctggcaac tggaaaggac tccccattta ttatatggag aaccagggga
      841 tatctggtta acggtccttc cggtatggca tagctttgaa cggctcatgc aatatatcgt
      901 tattgaacga tcgagcggaa tggtgtattc aaagccggtt gcctctgtta tgttccccga
      961 tatagctgcc atgaaaccgc acgttattcc cggggttcct cgcttatggg acgcccttgc
     1021 gaacggtatt ttgcgcactg tacgaaaaga aggcggcctt aaaaaagccc tgtttttctt
     1081 tttcctttct gtaggaaaaa agtattgcac cgcccatgat ctggtctttg gtagggttgc
     1141 ccgttttact cgtcgcgtta gagccttgga cacccttgtt ggcttgattc cctggctctt
     1201 gcttttattc cctcgcttcc tgggtaatgt gttggtgtat cgcaaaatca gacaaaaact
     1261 gggtggccga atccggtttt gtatctccgg aggtggggcc cttcagaaag atgtagacgc
     1321 cttttaccgg gctatcgggc ttaaagttat tgaagggtat ggaattacag aaaccgcacc
     1381 ggttttatcg cttagggatc aatggaagcc tcgccccggt tgtgtaggcg cggtttttac
     1441 tgacactgaa tgccggattg tagacagcga tgcgttggat agtgcaattt cttccgcgaa
     1501 agatgggctc tggagggttc ctcctgcctt accgccgggg aaacagggtg ttatcgtagt
     1561 tcgtggtggc caggttatga aaggctacta taaacgggac gatttgacta aaaaagcaat
     1621 tgatgctgat ggatggttta acactgggga cctgggtatg ctctcgtggg ataacgaga
//
LOCUS       DAOQXQ010000449         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_88076_length_1678_cov_2.835490, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000449 DAOQXQ010000000
VERSION     DAOQXQ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88076_length_1678_cov_2.835490"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1025)
                     /locus_tag="PKH81_07420"
     CDS             complement(36..1025)
                     /locus_tag="PKH81_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ97904.1"
                     /translation="MNRIFVKPELTYLNGKAWLAVPDFQHNLFSELSIDPLKPRQGVS
                     IWGDLVYCPSCSVPAFWHRTRLDKPFTAHFSSIKEAADILRSIQRNWAHYPVSSFRRA
                     ALITEKLPFIRDKPRVYPYIVPQNPMGLWSLLDDHTLFASAETSSPFPRGEIQFEEDH
                     ENPPSRAYLKMYEALAWVSYLYPSSEPTTRYILPSGARCVDAGACPGGWTWALTKLGA
                     KVTAIDRSPLAPSLMAHPDVTFIKHDAFTLTPQELGPQDWVCSDVICYPPRLLEWVNK
                     WLASGLCENFICTIKMQGETDHKTTQEFAAIPGSHIMHLTANKNELTWIRIGK"
     gene            complement(1000..1368)
                     /locus_tag="PKH81_07425"
     CDS             complement(1000..1368)
                     /locus_tag="PKH81_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNQ97905.1"
                     /translation="MLTVRMSREKRLTPRFRDFAKARVDELCQLPGYLEDISRTGCKV
                     RFPHTFPVDAEREYTLTVLPAGKSGLKEFDLTVKPEWISPDAEPFEVGFSVLCSPGLH
                     QLRKYVEMLAALDEQDICEA"
     gene            1437..>1678
                     /locus_tag="PKH81_07430"
     CDS             1437..>1678
                     /locus_tag="PKH81_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97906.1"
                     /translation="MAYRIRILSLCAFCLVSSVCIGIFSSCDFFSSDNPFPDEYYTAN
                     FIASIGFNQFAGAESASSQVIQARWDLAYRYLSWNG"
BASE COUNT          426 a          377 c          432 g          443 t
ORIGIN      
        1 gcgaatttgt gcgttgaggg acagagcatt ccaggttatt tcccgattcg tatccaggtc
       61 agttcatttt tattagccgt aagatgcatg atatggcttc cgggaatggc tgcaaattcc
      121 tgtgttgttt tgtggtctgt ttcaccttgc atcttgatgg tgcagataaa gttttcacat
      181 aaaccggagg caagccactt attcacccac tcaaggagcc ttggcggata gcaaataacg
      241 tcagagcaca cccaatcctg agggcccaac tcctgcgggg ttagggtaaa ggcgtcgtgt
      301 ttaataaaag taacgtccgg atgagccata agagaggggg caaggggcga tctatctata
      361 gcagtgacct tggccccgag tttggtaagc gcccaggtcc agcccccggg gcatgctcct
      421 gcgtccacgc atctagctcc gctggggagt atatatctgg tggttggttc ggaagaagga
      481 tagagatacg acacccatgc aagagcctca tacatcttaa ggtacgctct actgggcggg
      541 ttttcgtggt cttcttcaaa ctgtatttct cctcggggga aagggctcga ggtttcagca
      601 gaggcaaaaa gagtatggtc atccagtaag gaccaaagcc ccatcgggtt ttgaggaaca
      661 atataggggt atactctcgg tttatcacgt ataaacggaa gtttttctgt aatcagagct
      721 gctcgccgga aagaactgac aggatagtga gcccaattac gctgaattga cctcaggatg
      781 tcggctgctt cctttataga agaaaaatga gcggtaaaag gcttgtcgag tcgggttcga
      841 tgccaaaaag cggggacaga gcatgaaggg caatacacta aatcacccca gatggacacc
      901 ccttgtcggg gtttgagcgg gtcgattgac agttcggaaa acaaattgtg ctgaaaatct
      961 ggaacggcaa gccaggcctt tccgttcaaa taggtgagtt caggcttcac aaatatcctg
     1021 ttcatccaaa gcagcaagca tttcaacgta ttttcgcagt tgatgtaatc cgggggaaca
     1081 aagtacggaa aaacccacct caaaaggctc tgcatcggga cttatccact ctggtttaac
     1141 cgtaaggtca aactccttta gtcctgattt acccgcgggc aagacggtaa gggtgtattc
     1201 ccgttcggcg tcaaccggga aggtgtgggg aaaacgaact ttgcaaccgg ttctgctgat
     1261 atcttcaaga tagcccggca actgacatag ttcgtcaacc cgtgctttcg caaaatcgcg
     1321 aaatcgcggg gtcaatcgtt tctctctact catacggacg gtcagcattg acctttttca
     1381 accgtttgtc aataactggg gcttggggta tacttataca taaggatgga aagaagatgg
     1441 catatcgaat ccgcatcctc tctttatgtg cattttgttt ggtaagctca gtatgtattg
     1501 gcatattctc atcatgcgat tttttttctt ccgataaccc ctttccggat gaatactata
     1561 cggcaaattt tatagcctct attgggttta accagtttgc cggagccgaa agcgcttcca
     1621 gtcaggtcat tcaggctcga tgggatcttg cgtatcggta tctttcctgg aatgggta
//
LOCUS       DAOQXQ010000450         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_88494_length_1673_cov_3.147095, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000450 DAOQXQ010000000
VERSION     DAOQXQ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88494_length_1673_cov_3.147095"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKH81_07435"
     CDS             <1..734
                     /locus_tag="PKH81_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ97907.1"
                     /translation="RQTLKEQAIDVPAQDCFTRDNVQVRVDGVLYLQVVDPKKASYGI
                     RDYATATVLLAQTTMRSVVGQLELDSTFEAREQINATVVKAVDEASDPWGVKVTRYEI
                     QNIRVSQSIMDAMEAQMKAEREKRAEIARSVGEMETKINLSLAALEEAVNLSDGQRQR
                     LINEAEGEASEIVAVAEATALGIKKIAASISLSGGEEAANLNLSEAWIEAVGGIAKSG
                     SKVILSSDLTDLRDVVGKASRMITK"
     gene            complement(956..>1673)
                     /locus_tag="PKH81_07440"
     CDS             complement(956..>1673)
                     /locus_tag="PKH81_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97908.1"
                     /translation="TTPTTESTIPTIDSLVGTWDLQTCDALRGGNTHTTTREMEITKT
                     GPTTATFIMTATSVDKDSAGAVVDTWYMGQKGTFSLTTEGILTKSVLEIYDSRDPLTA
                     SSTWDDGEGMTQVYPIGFINSILYDGVYYRQGSGTGLTGTWKLLIHEGDDYYEEFTRG
                     TITLTSDTITQLSEESDDGTTWTVTASFGPAPCTVTTTFLQITSSGATQEILYYQQGN
                     CLFLPRTGETSTTNFGWVKR"
BASE COUNT          400 a          378 c          430 g          465 t
ORIGIN      
        1 atcggcaaac gcttaaggag caggcgattg acgttcccgc gcaggattgt tttacccgcg
       61 acaacgtgca ggttcgcgtt gacggtgtgt tgtatcttca ggtagtagat cccaagaagg
      121 cgagttacgg tattcgagat tacgcgaccg cgacggtgtt gcttgcacaa acgacgatgc
      181 gttcggttgt gggtcaactt gaacttgata gtactttcga ggcacgtgag caaattaacg
      241 cgacggttgt taaggcggta gacgaggctt ccgatccctg gggcgtcaag gttacccgct
      301 atgaaattca aaacatcagg gtttcccagt ctattatgga cgcgatggag gcgcagatga
      361 aggccgagcg cgaaaaacgt gccgagatcg cccggtcggt tggagagatg gaaaccaaga
      421 taaatctttc ccttgcagcc cttgaagaag cggttaactt atccgatgga cagcgccagc
      481 gcttgattaa cgaggcagaa ggagaggcaa gcgagattgt cgccgttgcc gaggctacag
      541 cccttggtat taaaaagatt gccgcttcca tcagtctctc cggtggagag gaagcagcaa
      601 acttgaacct ttccgaagcc tggatcgagg ccgttggcgg catcgccaag agcggctcca
      661 aggttatctt gagttcagat ttaacagatc ttagggatgt agtcgggaag gcttcccgaa
      721 tgattaccaa ataaaaaaag agggcagtct gaaaagtttt caggctgccc ctttacgtta
      781 cggttgtttg cttttctttt ctttcttaag ttcgcgcttt tcttttaggg tgtgctgagg
      841 ttttttcttg tcctcggcct ttcccttgtc ttttcccttt ggcatactag atacctcctc
      901 ttgacgggtc attacccgaa gtataccgct gggtgtacga tttgtacaga aaaaactatc
      961 gctttaccca tccaaaattg gtagtgctgg tttctcctgt tctgggcaag aagaggcaat
     1021 ttccctgttg ataatagagt atctcttggg tagcgccgga acttgttatc tgtaaaaaag
     1081 tcgtggtaac ggtacatggt gcagggccaa atgatgccgt aactgtccat gtggtaccgt
     1141 catcagattc ttcagataat tgggtaatag tatccgaggt taaggtaata gtccctctgg
     1201 taaattcctc ataataatca tcaccctcat gaataagcag tttccaggta ccggtaagtc
     1261 cagtaccgct cccctgccga taatagaccc catcgtacaa aatagaatta ataaaaccaa
     1321 ttggatacac ctgagtcatt ccttccccgt catcccatgt gctggaagcg gtgaggggat
     1381 cgcgagagtc gtatatttcc aaaacactct tggtcaggat gccttctgtt gtcagtgaaa
     1441 aagttccttt ttgtcccata taccaggtat caactacagc tcctgcagaa tctttatcaa
     1501 ctgaagtggc tgtcatgata aaagtcgcag tagttggtcc ggtctttgtg atctccatct
     1561 ctctggttgt tgtgtgggta ttacctcctc ggagagcatc acaggtttgt agatcccagg
     1621 ttcctacgag gctatctatg gttgggatgg tgctttctgt cgttggcgtc gtc
//
LOCUS       DAOQXQ010000451         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_89041_length_1667_cov_2.859181, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000451 DAOQXQ010000000
VERSION     DAOQXQ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89041_length_1667_cov_2.859181"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PKH81_07445"
     CDS             <1..1438
                     /locus_tag="PKH81_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNQ97909.1"
                     /translation="DAMMAVDITDQEMKAWLYATPPGPDGADLSADMIITFLKNNRVV
                     AGIDEQRISDFVDRPIYKQNYLVAEGLKPQDGADAHIMYNFETDRSKIRLKESASGKV
                     DFKELNLVQNVVEGQPLAKKIEAERGKAGKTVTGKYLEAKNGRDIPLPLGKNTKVADD
                     GLTIVAETNGQVLILNNKINVEPILNIEGDVSLKTGNIMFLGTVYISGNVEDGFSVKA
                     SGNIEVKGTVGKSELDAEGDIVVSQGIAGKGEGFIRSGKSIWSKFIENTKVEAGEFVI
                     VSDGIINSHVAANRKILCQGKRAAIIGGSLSAAEEIHAKTLGSYGGASETTLNVGFDP
                     RSKERFDALVANKQAAERELEDVQLNFNTLMNLKKQKKELPEDKEASLKKLGERQYIL
                     QTELEEIETELKQIQNYLNTLKTKGRVSASIKVFAGVKIVIRDVTEEVRSDCKATTFF
                     LENGLIRYGKYQGPDDDMKRVPSGYSAD"
     gene            1419..>1667
                     /locus_tag="PKH81_07450"
     CDS             1419..>1667
                     /locus_tag="PKH81_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97910.1"
                     /translation="MGIQPIDLQTLYAQLEKVGKAQGQQQAAQQLLREEEQARSRKEA
                     EERLRTVGETAAGNEGAGKVHERNGSSPQASGDSSQREK"
BASE COUNT          526 a          313 c          437 g          391 t
ORIGIN      
        1 cgatgccatg atggcagtgg atattaccga tcaagagatg aaggcatggc tttacgcgac
       61 ccctcccggt cctgatgggg cggatttgtc tgcagatatg atcattacct ttctcaagaa
      121 caatcgagta gtcgccggga tagatgagca aaggatcagc gactttgttg atagaccgat
      181 ttataagcaa aactatcttg tcgcagaggg ccttaagcct caggacggcg ctgacgctca
      241 tattatgtat aattttgaaa ctgatcgatc aaagattcgc cttaaagaaa gcgcttcggg
      301 taaggttgac tttaaagagc ttaatcttgt tcaaaacgtg gtagaaggcc agcccctggc
      361 aaagaagata gaagcagagc ggggaaaagc tggtaaaacg gtaaccggca aataccttga
      421 ggcaaaaaat ggtcgcgata tcccgcttcc tcttggaaaa aacaccaagg ttgcagacga
      481 cggtttaact attgtagccg aaacaaacgg tcaggttctt attttgaaca acaagatcaa
      541 tgttgagcca atattgaaca ttgaaggtga tgtttccctt aaaacaggta acatcatgtt
      601 cctgggaact gtgtatataa gtggtaacgt agaggatgga ttttctgtta aggcgtcggg
      661 aaacatcgag gttaagggaa ccgtcgggaa aagcgagctt gacgcagagg gtgatatagt
      721 cgtcagtcag ggaattgcag ggaaaggcga gggctttatt cgctccggta aatctatttg
      781 gtccaagttt atcgaaaata ccaaggttga agcaggagag ttcgttattg tatcagatgg
      841 tataatcaac tctcatgttg ctgcgaaccg caagatactc tgtcagggca agagagccgc
      901 aattataggt ggaagcttaa gcgcggcaga agagattcac gctaaaaccc tgggtagtta
      961 tggcggagcg agcgaaacaa cacttaatgt cggctttgat cctcgcagta aagagcgatt
     1021 tgatgccctt gttgccaata aacaggctgc cgagcgtgag cttgaggatg tacagcttaa
     1081 ttttaatacc ctgatgaatc ttaaaaagca aaaaaaagaa cttccggaag acaaagaagc
     1141 atccttaaag aaattgggtg aacgccaata tattttacaa acagaattgg aagaaataga
     1201 gactgaactt aaacagatac aaaattacct gaacaccctt aaaaccaagg gtcgtgtttc
     1261 tgcgtcgatt aaggtttttg ctggcgtcaa gattgttatt cgtgatgtga ccgaggaagt
     1321 acgatctgat tgtaaggcaa ccacattctt tttagaaaat ggcttgatac gatacggaaa
     1381 gtatcaggga ccggatgacg atatgaagag ggttcctagt gggtattcag ccgattgatt
     1441 tacaaaccct gtatgcccaa ctagaaaagg ttgggaaagc gcagggccaa caacaagctg
     1501 ctcaacaact gttgcgggaa gaagagcaag ctcgttcgcg taaggaagca gaagagcggt
     1561 taagaaccgt tggtgaaaca gctgcaggaa acgaaggtgc cggcaaggtt catgaacgca
     1621 atggatcgag tcctcaagct tcgggagaca gtagtcaacg ggaaaaa
//
LOCUS       DAOQXQ010000452         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_89166_length_1666_cov_1.965239, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000452 DAOQXQ010000000
VERSION     DAOQXQ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89166_length_1666_cov_1.965239"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKH81_07455"
     CDS             <1..769
                     /locus_tag="PKH81_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97911.1"
                     /translation="KGNEKGRVERAIRDIRSFVESNDFTDIRDLNEKTDRWRREWNVR
                     THRTTHTAPASMLTNERLLPLPVISYKPYRIIQVMISTTAFVEFDTNRYSVPTDYAAT
                     AASVLAYPDHLEIVVHDRKIAYHARSFGKYQKIEHPAHRQKLLDRTPQAKYERINHLM
                     TRMGSEIDHFLTVTESKGEDRLGIAYRLFCLLKFSSKEMLLSVVREAVSHQHTIRFIE
                     DTLAPRFFDRTAVYPQNTSLLDISYDRRELSDYDSLA"
     assembly_gap    877..886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            935..1492
                     /locus_tag="PKH81_07460"
                     /pseudo
     CDS             935..1492
                     /locus_tag="PKH81_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          457 a          414 c          411 g          374 t
ORIGIN      
        1 caagggtaat gagaagggac gggtggagag ggccatacgg gatatacgca gcttcgtaga
       61 aagcaatgat tttaccgaca tccgcgatct caatgagaag acggaccgat ggagaagaga
      121 atggaacgtc cgtacccacc gtacaacaca cacggcgccg gcatcaatgc tcacgaatga
      181 gcggcttctt ccgttaccgg ttatctcgta caaaccatac cggattatac aggtaatgat
      241 cagtacgacc gccttcgtag agttcgatac gaaccgctac agcgttccca cggactacgc
      301 cgcaacggcg gcatctgttc ttgcctaccc cgatcatctg gagatcgtcg tccatgacag
      361 aaagatcgcg tatcatgcga ggtccttcgg gaaataccag aagatagaac atcccgccca
      421 cagacagaag cttctcgacc gcacccccca agcaaagtat gaacggatca accaccttat
      481 gacgcgcatg ggaagcgaaa tcgaccattt tctcaccgta accgaatcga agggcgaaga
      541 cagactcgga atagcgtatc gcctcttctg tcttcttaaa ttctcatcaa aagagatgct
      601 cctctctgtc gtacgggagg cggtaagcca tcagcatacc attcgcttca tcgaggatac
      661 tttggccccc cgcttttttg atcggacagc ggtgtatccg cagaacacct cattacttga
      721 tatctcttac gacagacggg agcttagcga ctatgacagc cttgcatgag ttattaaaag
      781 agttcaggtt cctcgccggt tgcgaaagcc tctcatcaga tgaggaggct ttcctggaga
      841 ggttcctcac ccgtgaacaa tacttacgga aacagannnn nnnnnnaaaa gggaataacc
      901 ggcatgttaa gcacgcttaa aacagaagaa acaggaatac ctcctgcgca tgtcgggaat
      961 caagcgggta aagctgctct ctgattttga ctgggcgttc aatccgaaaa taccgaggga
     1021 gaagttcatg gagttcctga gaaccgattg gctcgtaaaa ccgtgtaatc tcgtattgat
     1081 cgggccaagc ggcaccggga agacgcacgc agcgagcgcg ctgtgttatg atgcggtgac
     1141 aaaagggaaa caaacagtct ttctcactct ctttgacctg actgcaaaac tgacgaaggc
     1201 gcggagtaca tatactctta tcgactacta tgcgaaagcg ccggtgctct gtattgatga
     1261 agtcggctac gtcatgcctt cacgggagca tgccgattat ctcttccaga tcatctcaaa
     1321 acgaggtgag accggtacca ctatcgtgac gacaaatctt gttccttctc agtggggaaa
     1381 ggtcttcgac tcaaccaccg catccgccat actggaccgg ttgtctatga acggcacgtt
     1441 catcaccttt gaagggaggt cctataggaa cagaaaaaac ggggcgatgt gatcgtattc
     1501 atctaattgc acacaaaatg atgcaacctc tctgcataaa gagacgagcg tttggggatg
     1561 aggactatct tgccatcaga aatgagggta ttgacatcgt cagaaatgag ggcggattga
     1621 ttggcgctaa caagcgtcaa gcactttttg tttatctgta tttttt
//
LOCUS       DAOQXQ010000453         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_89491_length_1662_cov_2.177971, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000453 DAOQXQ010000000
VERSION     DAOQXQ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89491_length_1662_cov_2.177971"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKH81_07465"
     CDS             <1..1268
                     /locus_tag="PKH81_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97912.1"
                     /translation="LVRFLSFIERWDLSGMPIYCAMTDLSPIVFCHSLFGWGEGMFGG
                     YPYFVGVHRLLKKKGASLPPALFPSTGPISSLHDQACELFYQLKGGRVNYGKAHSQKY
                     GHAQYGRTYDIPLYSIWDSAHPLDFVGHSMGAPVIRMLQHLLAENFFSAEDECDYSCS
                     ASWVRSLTSVAGVHNGSTLTWVLGFDEDTSEVPSNAWAIQLITRAFSKYAALQARKRE
                     SADFYDLHLEHWGIANARDLGFSIERVLSDPRFMLSGDWAFHDLTPNAMEAFNRRTRD
                     YPDTRYYSFSIRSTISLPIGISLPVPYATHFFLIPFSFAMGKYKCASKKWKAVFSRSW
                     RANDGMCNTWSQNCPKLGREDTYGRFPRPVRYRLTSKKIPPKGVWLWVSSLRFVDHAE
                     VAMLPHLLRLSFSTRFYRRIFYLVTKTRE"
     gene            1271..>1662
                     /locus_tag="PKH81_07470"
     CDS             1271..>1662
                     /locus_tag="PKH81_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97913.1"
                     /translation="MAEDCVKCLLRSDQMIKPDEIPLMTQYIPNRLARTIGELPCCFF
                     ERKLEPLTGMVAYFDIVGFTPIVANHVNSGRDVALLSTIFRNYYSTIIETIKLMGGSV
                     YQFAGDSLLICFESLYGESEKETWDRNLS"
BASE COUNT          385 a          384 c          392 g          501 t
ORIGIN      
        1 ccctggtaag gttcctgtct tttatagaaa gatgggattt aagtgggatg ccgatttact
       61 gcgccatgac tgatctttcc ccaattgttt tttgccatag cctgtttggt tggggagagg
      121 gcatgtttgg cgggtacccc tattttgtag gtgtccatcg tctattaaaa aagaagggcg
      181 cctcgctccc tcctgcgctt ttcccttcca ccgggcctat ttcctccctg catgatcaag
      241 cctgtgagct tttttatcaa ttaaagggcg gcagggtaaa ctacggtaag gctcatagtc
      301 agaagtacgg ccatgctcaa tatggaagaa cctacgatat ccccttgtat tccatatggg
      361 atagcgctca tcctcttgat tttgttggcc actctatggg ggccccggta atccgcatgc
      421 ttcagcattt attagcggag aatttttttt ctgcggaaga tgaatgtgat tatagttgct
      481 ccgcatcctg ggttcgctcc cttacctcgg ttgctggggt tcacaatggc tctaccttaa
      541 cctgggtatt gggttttgat gaggatacct cagaggtacc ttccaatgcg tgggcgatac
      601 agctaataac cagggctttt tcaaaatacg ctgcgttaca agcgcgaaag cgcgaaagcg
      661 cggattttta cgatcttcat cttgagcact ggggtattgc gaacgcccgg gatcttggat
      721 tttccatcga acgagtgtta tccgatcccc ggtttatgct aagcggtgat tgggcttttc
      781 atgatctaac acccaatgcc atggaggctt ttaatagacg tacccgtgat taccccgata
      841 cccgctatta tagtttttcc attcgctcta cgatttctct tccgatcggg atttccttac
      901 cggttcctta tgcgacacac tttttcttga tcccgttttc ttttgcgatg ggtaaataca
      961 agtgcgcaag caaaaaatgg aaggctgtgt ttagccgttc ctggagagcc aatgatggca
     1021 tgtgcaatac ctggtcgcaa aactgtccca agcttggaag ggaagatacc tatggaaggt
     1081 tccctcgtcc agttcgctac cgattaacat ccaaaaagat tcccccgaaa ggcgtgtggc
     1141 tgtgggtttc ttcgcttcga ttcgtcgacc atgcagaggt tgccatgttg ccacaccttt
     1201 tgcgtctctc tttttccact cgtttctata ggcgcatatt ttatttagta acaaaaacca
     1261 gggagtagta atggcagaag attgcgtaaa gtgtctgttg cgaagcgatc aaatgattaa
     1321 gcccgatgaa attccgctga tgactcagta cataccaaac cgcttggcaa ggacgatagg
     1381 ggagttaccc tgttgttttt ttgaacgaaa acttgagcca cttaccggca tggtcgcgta
     1441 ctttgatatt gtcggtttta ctcccatagt ggcaaatcat gttaactcag gaagggacgt
     1501 agcactgcta tctactattt ttcgcaatta ctattctacc atcatagaaa ctatcaaatt
     1561 aatggggggc tcggtatatc agttcgcggg agattccctg cttatctgtt tcgaatcact
     1621 gtatggcgag tccgaaaaag aaacctggga tcgaaatctt tc
//
LOCUS       DAOQXQ010000454         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_89781_length_1658_cov_2.941360, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000454 DAOQXQ010000000
VERSION     DAOQXQ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89781_length_1658_cov_2.941360"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="lysS"
                     /locus_tag="PKH81_07475"
     CDS             <1..1344
                     /gene="lysS"
                     /locus_tag="PKH81_07475"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ97914.1"
                     /translation="NMPKPEELEKFLRFPITMVPDTFGRDESYARHHEVDVESVLPTV
                     GITPEFLYQAERYRSGMYAEGMKTALNNRARIKDCLNRYRDEAHQIPEAEEYWPVSIF
                     CNACNRDTTSITGWDGEWGLSYSCECGHSETVDIRSAKGVKLGWRVDWPMRWKHEKTV
                     FEPAGKDHHSQGGSFDTARLVSKEIFDWEAPVTFRYDFIGIKGTPGKMSSSKGKVVDL
                     PDILRVYQPEIARYLFAGTRPNTEFVISFDLDVIKIYEDYDKTERIAWGLEKAKNEEV
                     YAKERRIWELSQVGELPSMASYQVPFRHLCNLLQTNDGDIDAVIASFGDVHPDQVERL
                     KTRSRCAWYWITECAPEDFRFALRKDGSVATLGVNELTAVRKIRDEVLPKMDGVDEKG
                     LSELVYAVAQEVGLEPKALFTAVYQALIAKDQGPRLASFMKIIGKERIASILASY"
     gene            1410..>1658
                     /locus_tag="PKH81_07480"
     CDS             1410..>1658
                     /locus_tag="PKH81_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97915.1"
                     /translation="MLEKKPLITDLYSADPSANVFNGKLYIYPSHDRDIEMESNDNGD
                     QYDMEDYHVYEMEDMNTPPRDCGIALHIKDIPWASKQVW"
BASE COUNT          406 a          387 c          446 g          419 t
ORIGIN      
        1 aatatgccca aaccggagga gcttgaaaag tttcttcgct tccccattac catggttccc
       61 gatacctttg gtcgcgacga aagttatgcc cgccatcatg aggtggacgt tgaatcggta
      121 ttgcccactg tcggcattac gcccgaattc ctgtatcagg ctgaacgcta tcgcagcggt
      181 atgtatgccg aagggatgaa gacagcccta aacaacaggg ccaggattaa ggattgcctt
      241 aatcgctatc gggatgaggc tcatcaaatt cctgaggccg aggagtattg gcctgtctcc
      301 atattctgca atgcgtgtaa ccgggacaca acctcgatta ccggctggga tggtgaatgg
      361 ggtctttctt attcatgtga atgcgggcat agcgaaaccg tggacattcg tagtgcaaag
      421 ggcgttaagc ttggttggcg ggtagactgg cccatgcgct ggaaacatga aaaaacggtc
      481 tttgaaccgg ccgggaagga tcatcattcc cagggaggtt cattcgatac cgctcgtctt
      541 gtatccaagg aaatttttga ctgggaggcg ccggtaacct tccgctatga ttttattggg
      601 attaagggta ccccgggaaa gatgtcttcc tccaagggaa aggttgtcga tttgccggat
      661 atccttcgcg tgtatcagcc ggaaatcgcc cgctatctgt ttgccggaac ccgaccgaat
      721 accgaatttg ttatcagctt tgaccttgat gttatcaaga tttacgagga ttacgacaag
      781 accgaacgta ttgcctgggg tctggaaaag gccaagaacg aagaggtgta cgcaaaagaa
      841 cgccgtattt gggagttatc ccaggttggc gagttgccct ccatggcttc gtatcaggtc
      901 cccttccgtc atttatgcaa tcttttgcaa accaacgatg gagatataga cgcggttatt
      961 gcctcctttg gggatgttca ccccgatcaa gttgaacggt taaagactcg ctctcgctgc
     1021 gcctggtatt ggattaccga atgcgcacca gaagacttcc gtttcgcgct caggaaggat
     1081 ggttcagtcg ccactttagg agttaacgaa ctgaccgcag tgcggaagat tcgcgatgag
     1141 gtgcttccca agatggacgg cgtggatgaa aaaggcttgt ccgagctggt ctatgctgtt
     1201 gcccaggagg tcggccttga gccaaaggcc ctgtttaccg ccgtgtatca ggcgcttatt
     1261 gccaaggacc aagggccacg gcttgcctcg tttatgaaga ttattggtaa ggagcgaatc
     1321 gcttctatcc ttgcttcgta ttgaatcgtt agcatcaaaa cgccgggtag tgtatactac
     1381 aaggtataat ttacaggggg tataacacca tgcttgagaa aaaaccactt attaccgatt
     1441 tgtacagcgc cgatccttcg gcgaatgtgt ttaatggaaa actgtatatc tatccttccc
     1501 acgaccgcga catcgagatg gagtccaacg ataacggcga tcagtacgac atggaagatt
     1561 atcatgtgta cgagatggag gatatgaata cacctccccg tgattgcggt attgccctgc
     1621 acattaagga tatcccgtgg gcttccaagc aggtgtgg
//
LOCUS       DAOQXQ010000455         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_90141_length_1654_cov_2.554722, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000455 DAOQXQ010000000
VERSION     DAOQXQ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90141_length_1654_cov_2.554722"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1654
                     /locus_tag="PKH81_07485"
     CDS             32..>1654
                     /locus_tag="PKH81_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97916.1"
                     /translation="MAAIFLLLLYASPSALVLYWTANNLFSLIKTAILQTKNPRKICY
                     AILWVSLTFLCVYIAFFRHHSRSYRLRNMILTFSVAGILAAIPLYLRFIVWTGKKCIP
                     PYSGTEVSYRALFLSASAALALLMGLYIPITVMASSPFEFSFLGDTAHPFEILLFPLA
                     QSIGLFFVWPTCLYLLFSQRIKRLLTVISVSTLVYAISFSMIAPSPAGSLTAALRFIE
                     GGAFIVSSATEIRSVLIALLLLSLTAFLITKGKLSLLTKVSLLCAVSFMALGVYRGRI
                     LLSGYSDKKMILSRESDSQSTHGAIPKKISLSLDGHNVVLIMLDKAISSYLPLILEER
                     PELQEIFEGFVYYPNTLSFGQKTIIGAPPLFGGYEYTPQAMNLRSNEPMVDKHNEALL
                     VLPTLFMKNGYSTLITDAPFVNYQWIADGDFFRSRGIGATNLQGTLSSRYIHDYFGEI
                     TALGPDTLLKRNFIIFSLMSAAPHRLREALYNDGRYWNSTLLTLNTHTLDSYAVLHYL
                     PDLTSFTASGNTFTSITNDLTHEPSLLSWPDYT"
BASE COUNT          386 a          432 c          320 g          516 t
ORIGIN      
        1 tcctcttcgg gaaaaaatcc aattatacag catggcagct atttttcttc tcttgctgta
       61 cgcatcacct tctgcacttg ttctctattg gacagccaat aatctttttt ctctcatcaa
      121 gactgctata ttacagacga agaatccccg taaaatttgt tatgctattc tctgggtctc
      181 tcttaccttc ctctgtgtat atatcgcttt ctttcgccat cacagcaggt cctatcggct
      241 cagaaacatg atccttactt ttagcgttgc aggtattctt gccgcaatac ccttgtatct
      301 tcgctttatt gtgtggacag gaaaaaaatg tattcctcct tattccggga cagaggtatc
      361 ctatcgagcc ctgttcctga gcgcatcagc agcgcttgcc ctgttaatgg gattatatat
      421 cccgataacc gtcatggcgt cctctccctt cgaattttct tttctgggag acaccgcaca
      481 cccctttgaa atccttctct ttccgttagc tcaatcgatc ggtttatttt ttgtctggcc
      541 aacgtgcctg tatttactct tttctcagcg aatcaagcgt ttgcttaccg tcataagcgt
      601 cagtaccctt gtatacgcga tttctttttc tatgattgcc ccttcgcctg caggatcatt
      661 gactgcggcc cttcgtttta ttgaaggggg agcgtttatc gtttcatcgg ctactgaaat
      721 tcgttctgtc ctgatagcgc ttctcttgtt gtctcttacg gcctttttaa ttacaaaagg
      781 aaaactgtct ctcctgacca aggtatccct gttatgcgcg gtatcattta tggcccttgg
      841 cgtgtatcgg ggaagaatac tactgagcgg gtattctgac aaaaaaatga tcctgagcag
      901 ggagtcggac agtcaatcaa cacatggcgc gattccaaaa aaaatatcgc tttcccttga
      961 tgggcacaat gtcgttctca tcatgttgga taaggcgatc agttcttacc tgccgcttat
     1021 cctggaagag cgaccggagt tacaagagat tttcgagggc ttcgtctatt atcctaacac
     1081 cctatcattt ggccaaaaaa ccattattgg cgctccaccg ctttttggcg gttacgaata
     1141 tacaccgcag gcaatgaacc ttcgcagtaa tgaacctatg gtggataaac ataatgaggc
     1201 acttctcgta cttcccaccc tttttatgaa aaacggctat tcaacactca taaccgacgc
     1261 gcccttcgtg aactatcaat ggatcgccga cggagatttt tttcgctcaa ggggaatcgg
     1321 agctactaac ctccagggca cgctctcaag ccgatacatc cacgattatt ttggagagat
     1381 aacggcactc ggccctgata cgcttttaaa gcgtaatttt attatcttct ccctcatgag
     1441 tgcagctccc caccgtcttc gtgaagccct gtataacgat ggacgatact ggaactctac
     1501 cttattaacg cttaataccc ataccctgga tagctatgcc gtattgcatt atctacctga
     1561 tcttacctcg tttactgctt cgggtaatac ctttacgagt ataaccaacg acttgacgca
     1621 cgagcccagt ctcctttcct ggccagatta cacc
//
LOCUS       DAOQXQ010000456         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_90500_length_1650_cov_2.555486, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000456 DAOQXQ010000000
VERSION     DAOQXQ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90500_length_1650_cov_2.555486"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..275)
                     /gene="csrA"
                     /locus_tag="PKH81_07490"
     CDS             complement(54..275)
                     /gene="csrA"
                     /locus_tag="PKH81_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNQ97917.1"
                     /translation="MLILSRKINEKIMIGEDISLTIIEIRGDQVKVGVEAPKSVKVFR
                     QEVYEAIQSENRAAAVSATNLDGLSNIVL"
     gene            complement(275..718)
                     /locus_tag="PKH81_07495"
     CDS             complement(275..718)
                     /locus_tag="PKH81_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNQ97918.1"
                     /translation="MDIRTKSMGTVSIVEKQIIDLGKGFYGFGEFHRFALIDATQKPF
                     VWVQSMDKEDLAFLAIDPFLFRPDYEIDIDDTLLQPLEVASPSDVIVFALVTVPTDGS
                     SVTANLQGPLIINKRNNRAMQAVLTDPRWHTKHDIVAESAAKRGR"
     gene            complement(731..>1650)
                     /locus_tag="PKH81_07500"
     CDS             complement(731..>1650)
                     /locus_tag="PKH81_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HNQ97919.1"
                     /translation="NISVEVDELLKELVSYGNATGPDGTRLFSGTKSFTEPFETVMGD
                     LNGAGEAMIREVRYNGSVDGKKIEADELTYLETEQAGNRTFWAERQSLFSELDAKDYS
                     VKADSVIEVDGVSINLTPGDTVHSIISKINDSGAAVKANLDPVTNGLNIETTDARQLW
                     LRDAAGSTVLSDLGMIKPDQRPPYNLSASVRVSGGSLFDAVIALRDSLFKGDQEAIGS
                     RVLGSIDGAVDNLSTRLAGIGSRYERAQATLSRIEVQKINVTAAESRESDLDFTKAVT
                     DLKMFEYTHQAALGVAGKLYSNTLLNYVK"
BASE COUNT          424 a          419 c          355 g          452 t
ORIGIN      
        1 ccccgaccgg acacccgtga ccgttcaaag gaattttccg aatcgctgta gctttataat
       61 acgatattgg aaaggccgtc caggttcgtt gcagaaaccg cggcggcgcg gttttcactt
      121 tgaatagcct cgtacacttc ttgacgaaac accttgaccg attttggcgc ttctacacca
      181 acctttacct gatctccgcg aatttcgata atcgttagcg agatatcctc accgatcatg
      241 attttctcat tgattttgcg cgacagtatc agcattatct gcctcgcttt gcggccgatt
      301 ctgccacaat atcgtgcttg gtatgccaac ggggatcggt taagaccgct tgcatggccc
      361 ggttgtttct cttgttgatt atgagcgggc cctgcaaatt cgcggtaacc gaacttccgt
      421 cggtagggac ggtaaccaat gcaaacacaa taacatccga aggagaggct acctccaagg
      481 gttgcagcaa ggtgtcgtca atgtctatct cgtaatcggg tctaaataaa aaaggatcaa
      541 ttgccaaaaa ggccaaatct tccttatcca ttgactgcac ccatacgaat ggtttttgag
      601 ttgcgtctat taacgcaaag cggtgaaatt caccaaatcc ataaaaaccc ttaccaagat
      661 caataatctg cttttctacg atggaaacgg ttcccatcga ttttgttcgg atgtccatgc
      721 ctgttcaacc ttatttgacg taattcagaa gtgtatttga atacagtttc ccggccacgc
      781 cgagcgcagc ttgatgtgta tactcgaaca tcttaagatc tgtaaccgct tttgtaaagt
      841 caagatccga ttcccgggac tctgcggcgg taacatttat tttttgaacc tctattctgg
      901 acaaggtcgc ctgcgcgcgt tcataacgag agccaatacc agctaatctt gttgataaat
      961 tatcgactgc gccgtctatc gatcccaaga ctcgactacc aatggcttcc tgatctccct
     1021 taaacaagga atcacgcagg gcgattaccg catcaaacaa agaaccgcct gagacgcgaa
     1081 cagaggccga aagattataa ggagggcgtt gatccggctt tatcatacca aggtccgaaa
     1141 gaacggtaga accggcagca tcccgaagcc aaagctgtct ggcatcggtg gtctcgatat
     1201 tcaatccatt ggttactgga tcaaggtttg ccttaacggc cgcgccagag tcgtttattt
     1261 ttgaaataat tgaatgaacg gtatcaccgg gagtaaggtt aatggatacg ccgtcaactt
     1321 cgattacgct gtcggcttta accgagtaat ctttcgcgtc aagctcggag aaaagacttt
     1381 gccgttctgc ccaaaaggtt ctgtttcccg cctgctcggt ttccagatag gtaagctcat
     1441 cggcctcaat tttttttccg tccaccgaac cgttataccg aacttcccgg atcatcgctt
     1501 ctcctgcccc gttcaagtct cccataacgg tttcaaatgg ctcggtaaaa ctttttgttc
     1561 ccgaaaaaag cctggtacca tcgggtcctg ttgcgtttcc ataagaaaca agttctttta
     1621 aaagttcatc aacctctaca gaaatatttt
//
LOCUS       DAOQXQ010000457         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_90874_length_1646_cov_2.806411, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000457 DAOQXQ010000000
VERSION     DAOQXQ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90874_length_1646_cov_2.806411"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1646)
                     /locus_tag="PKH81_07505"
     CDS             complement(<1..>1646)
                     /locus_tag="PKH81_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ97920.1"
                     /translation="NLDLLCIADTDGHFIRVNREWSNILGYTTEDLESRQFLDFVHPD
                     DLDSTLKTLSLLDEQKDVINFVNRYRCKDGSYRFIEWRSHPQGKRIYAAARDITDRKK
                     NDALLQSYLSMQEILIRMSSRFINIPLEHMDSAINDSLREIGTFIHSDRSYVFEYRWA
                     EQYCINTHEWCNAGTSSQMAKLQHVDVTDVPEWTAAHRSGSAIVISDVQTLDTDSRIR
                     HILLEQEVKSLLTIPMMERDECIGFIGFDWVHTLHEYSEKEKLLLTMFSDMLINFFNR
                     FKLEKNLVEARDLANSANQAKSDFLANMSHEIRTPMNAIIGLTSLVMDSTLTEEQYGL
                     LRKIDISSKLLMEIINNVLDFSKIEAGKMEIDEHPFSFETIIDQLQSLFSESAKEKNI
                     ELFLSLDSKIPRFLIGDSLRLYQVLANIVSNAIKFTENGSVTCSITLASAHGSETRVA
                     FTISDTGIGMNRDELAGLFRAFSQADSSTSRKYGGTGLGLVISSRLVSAMGGSISATS
                     QRGKGSTFSFELVFHIPVPSETDADNREIFVPSFTTQETG"
BASE COUNT          481 a          371 c          344 g          450 t
ORIGIN      
        1 ggcctgtttc ttgcgttgta aatgagggaa caaatatctc ccgattatcc gcatctgttt
       61 cggatggaac aggaatatga aacactagtt caaaggaaaa ggtactcccc tttccccttt
      121 gcgaggtagc agagatagac ccgcccatcg cagatacgag tctgctgcta ataacaagac
      181 cgagccccgt acctccatac tttcgcgatg ttgaagaatc tgcctgcgag aatgccctga
      241 aaagacctgc aagctcatcc cgattcatac cgataccagt atcagaaatc gtaaaagcga
      301 cacgagtttc actgccatga gcagatgcaa gagtaatcga gcaggtaaca gatccatttt
      361 cggtaaattt gatcgcgtta ctcacaatgt ttgcgagaac ctgatacagc ctcagagaat
      421 ctccaattaa aaagcggggg atttttgaat ccaaagaaag aaatagctca atgttcttct
      481 cttttgctga ctcggaaaaa agcgattgaa gctgatcaat aatcgtttca aaactgaaag
      541 ggtgctcatc aatctccatt ttacccgctt caattttgga aaaatcaagt acgttattga
      601 ttatctccat taatagtttt gaggaaatat ctatcttgcg caacaagcca tattgctcct
      661 cggtaagtgt tgaatccatc accagggaag tcaatccaat aatcgcgttc atcggagttc
      721 taatctcgtg actcatattc gccaaaaaat ccgactttgc ctggttcgca gagttcgcaa
      781 ggtcccgcgc ctcgactaaa tttttttcca gtttgaagcg gttaaaaaaa ttaatgagca
      841 tgtcggaaaa catggttaac aagagttttt ctttttcaga atactcatga agtgtatgta
      901 cccagtcaaa gccaataaaa ccaatacact cgtcccgctc catcatcgga atggtcaaca
      961 ggctttttac ttcctgttca aggagtatgt ggcgtatacg cgagtccgtg tcgagtgttt
     1021 gtacatcgct gatcacaatt gcagagccag atcgatgcgc ggcagtccat tcaggaacat
     1081 ccgttacatc aacatgctgt aacttggcca tctgggaact tgtgccagca ttgcaccatt
     1141 catgggtgtt tatacaatac tgttctgccc aacgatattc aaagacataa gagcgatcgg
     1201 agtgaataaa ggttcctatc tcccgcaatg aatcgtttat agcactgtcc atgtgctcaa
     1261 gaggtatatt gataaatcga gaagacattc tgataagaat ttcctgcatg gaaaggtagg
     1321 actgaagcaa cgcgtcgttt ttcttccggt ccgtaatatc cctggccgca gcataaatcc
     1381 gctttccctg tggatgagat ctccattcaa taaagcggta acttccatcc ttacaccgat
     1441 accgattcac aaagtttatt acatcctttt gctcgtccag aaggctaagg gttttaaggg
     1501 ttgagtcaag atcatcagga tgaacaaaat caaggaattg tctggattca agatcctctg
     1561 tggtatatcc gagtatgttg gaccattcac gattaacgcg aataaagtga ccgtctgtat
     1621 cggcgatgca caacaggtca aggttt
//
LOCUS       DAOQXQ010000458         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_90963_length_1645_cov_2.796855, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000458 DAOQXQ010000000
VERSION     DAOQXQ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90963_length_1645_cov_2.796855"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKH81_07510"
     CDS             complement(<1..540)
                     /locus_tag="PKH81_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ97921.1"
                     /translation="MPRCKPVASIPLLLSFVLLASCMAKGQHNTSNAPSSLTNPPERI
                     VSLAPATTSIIIALNATDRLVGVDSWSAKTHKIPSIIPQFDLIRPDAETLMALEPDVI
                     LLSSLTGEGKPEESLSPLIQRGIKIEIFHTSENLADVFADIQRIADLLGYSSKGQDLV
                     HRLTDEVEAIRASGAAIPEE"
     gene            644..1357
                     /locus_tag="PKH81_07515"
     CDS             644..1357
                     /locus_tag="PKH81_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ97922.1"
                     /translation="MKFILASDIHSNLPALEAFFSYLTSHGLSGEPVFFLGDYVNLGP
                     FPEETVSLLRTYPAQVFLSGNHDRYIINEHALDHNPYFGSPEGVTHCRWTREQLSPQS
                     IEWLKNLHTRHRFEALSWKCDLIHGRHGSDEETLDESKIDTTSRILYICGHTHVPRNQ
                     TVGKARIFNPGSLGKPLDRDNRASFGIVHLTEDDATFEIVRIPYDIERSVKALEDRKV
                     PWRGGIIQSLRTAVYTDEE"
     gene            1347..>1645
                     /locus_tag="PKH81_07520"
     CDS             1347..>1645
                     /locus_tag="PKH81_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNQ97923.1"
                     /translation="MKNNLCTVFLAGDVCASVGVRAVTNLLPKLIERESIDFCVINGE
                     NATGGIGLSSGDAEAILAAGADVITGGNHSFEKRDHWPVLDVNPAVLRPANFPSRG"
BASE COUNT          390 a          416 c          426 g          413 t
ORIGIN      
        1 ttcttccggt atcgcggctc cagaagcgcg aatcgcttct acctcgtccg ttagtcggtg
       61 aacaagatct tgcccctttg atgagtagcc aagaagatcc gcgattcttt gtatatcggc
      121 aaaaacatcc gcaaggtttt cgcttgtatg gaatatctct atcttgatgc cccgctgtat
      181 aaggggagaa aggctttctt ccggctttcc ctctccggtt aagctggaaa gaaggatcac
      241 gtcgggctca agagccataa gtgtttccgc gtccgggcgt ataaggtcaa attggggaat
      301 gatggaggga atcttgtgcg ttttggcgga ccatgagtcc acgccgacaa gccggtctgt
      361 cgcgttaagg gcaattataa tcgaagtggt tgccggtgcg agtgaaacaa tccgttcagg
      421 cgggttagtc aggctcgaag gagcattgga ggtgttgtgt tggcctttag ccatgcacga
      481 ggcaaggagg acgaaagaaa gaaggagagg gatggaggcg acaggcttac atcggggcat
      541 atagccacta tacagtcgtc caaaggcgca agcaaggggg tggaagtttt cttctgtttc
      601 cttgattgaa ttcccttagg aagtaggcta gaatagagcc ttaatgaaat tcatactggc
      661 ttctgatatc cactccaact taccggcctt ggaagccttc ttttcctacc tgacctcaca
      721 tggtctttcc ggcgaaccgg tattcttctt gggcgattac gttaatctag gtccctttcc
      781 cgaagaaacc gtctccctgc tcagaaccta tcccgcacag gtgtttttat ccggcaatca
      841 tgatcggtat atcatcaatg aacacgccct tgatcataat ccctattttg gcagtccgga
      901 aggggtaacc cactgtcgct ggactcgtga acagctttct ccccaaagca tcgaatggct
      961 taaaaattta cacacccgcc atcgcttcga agcgctctcc tggaagtgcg atcttattca
     1021 cggccgccat ggctcggacg aagaaacttt ggacgaaagc aaaatagaca ccacgtcgcg
     1081 catcctgtat atctgcggtc acacccatgt tccccggaat cagactgtag gcaaggcgcg
     1141 catttttaat cccggaagcc tgggtaagcc cctggaccgc gataatcgag cctcgttcgg
     1201 tattgttcac cttaccgaag atgacgccac cttcgagatt gtccgaattc cctacgatat
     1261 tgaacgatcc gtaaaagccc tggaagatcg aaaggtcccc tggaggggcg gcattattca
     1321 aagccttcgc actgctgttt ataccgatga agaataatct gtgcaccgta tttctggccg
     1381 gggatgtgtg cgcgagcgta ggcgtacgcg cggttaccaa tctactaccc aagcttatag
     1441 agcgagaatc cattgatttt tgtgttatca acggcgaaaa cgcgactggg ggtattggcc
     1501 tttcctcagg agacgccgaa gcaatccttg cagccggcgc cgatgtgata accggtggca
     1561 atcattcttt tgaaaaacgt gaccactggc cggttcttga tgtaaacccg gcggtattgc
     1621 gtcctgcaaa ctttccttcc cgggg
//
LOCUS       DAOQXQ010000459         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_91001_length_1645_cov_1.934591, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000459 DAOQXQ010000000
VERSION     DAOQXQ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91001_length_1645_cov_1.934591"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1645
                     /locus_tag="PKH81_07525"
     CDS             <1..>1645
                     /locus_tag="PKH81_07525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ97924.1"
                     /translation="DESYSIATSNHSFSHIISLVSRDSHPPLYSLVLRLAVLLFGHSL
                     LVIRGVSAMAVVGTVALAYAFLRRFWGSPNAIVFCIAFLITPMTIGAAQEARMYTLAS
                     FFVTGMILSGFAALTDNKKRDWAALVIFASLAGWTHYFALIASGMYWCVLLVKTIKTG
                     KPGGVKANLAKGDLLRRLLIAGAVVLALYLPWMFNLISQATRVAQNYWIGPITLNSVF
                     AILSFPFGQRFGGPHGMSSFNLFIIVQALSITGIVWAIKNKDKNAFLPLSALLTYWLT
                     FTCGILLSWAIRPIFVERYLITCLGALILAFSAFLIQFQIKRFTILGLIVYLFITFPI
                     LRSTYSTQVNGPGDTVAELYKDKVKPGDIFVHGSEHTFGILRYYFPDNLHYLYIPKDF
                     TPFGNHAVFAPNAEVGSDLRKYTAQPVTLWLVNRTGEYYSTPWMEIIGAPYREAVNKI
                     ERFNKKPGWLTMLIQEVRYNPDKIANGQESTTGQLTLNITGVNPALKGQVVYALYTSD
                     PIMPDNMAHSGAVNATAQTVPLFINNLTHGEYALFCFHDI"
BASE COUNT          383 a          449 c          352 g          461 t
ORIGIN      
        1 tgacgaaagt tattccatcg cgacctcgaa ccattccttt tcgcatatca tctctctggt
       61 gtcccgggac agccaccctc ccctgtatag cctggtcctc cgactcgcag tactcctttt
      121 tggccattca ttgctggtta tccggggtgt atcggccatg gcggtagtag gaacagttgc
      181 attggcgtat gcctttctta ggcgtttttg gggaagtccc aacgcgattg tcttttgcat
      241 cgcattcctc atcaccccca tgacgatcgg cgcggcgcag gaagcccgca tgtataccct
      301 tgcctctttc tttgttacgg ggatgatcct ttccggattt gccgccctta cagacaataa
      361 aaagcgagac tgggcggccc ttgtcatctt tgcttctctt gcgggatgga ctcactattt
      421 tgcccttata gcgtcgggaa tgtactggtg tgttcttttg gtaaagacta tcaagacagg
      481 aaaacccgga ggcgttaagg ctaaccttgc aaagggagat ctcttgcgac ggctcttgat
      541 tgccggagca gtcgttctcg cgctctatct tccctggatg ttcaacctga tcagccaggc
      601 aacacgggta gcacaaaact actggatcgg ccctattacc cttaactccg tattcgccat
      661 tctttccttt ccctttgggc aacgctttgg tggccctcat ggaatgtcct cgtttaacct
      721 gtttattatt gtccaagccc tatccattac aggaatagta tgggcgatta aaaacaagga
      781 caagaacgcc ttccttccgc tttcggccct tcttacctac tggcttacct ttacctgtgg
      841 tattcttctt tcatgggcaa tcagacccat atttgtagaa cgatatttaa tcacctgcct
      901 aggagccctt attcttgcct ttagcgcatt cttgatccaa ttccaaatta aacgctttac
      961 cattcttggc ttgatagtgt atctctttat cactttcccg attcttcgca gtacctactc
     1021 cacccaggtt aacggaccgg gagacactgt agccgaactc tacaaagaca aggtaaaacc
     1081 gggcgatatc tttgttcatg gcagcgagca tacctttggc attcttcgct actactttcc
     1141 cgacaacctt cattatctgt atatccctaa agactttacg ccctttggca atcacgcggt
     1201 ctttgcaccc aacgcagaag tcggaagcga tcttcgtaaa tacaccgcac aaccggttac
     1261 actgtggctt gtcaatcgta ccggggaata ttattcaact ccctggatgg aaatcatagg
     1321 ggcaccgtat cgcgaggcgg ttaacaagat agaacgattt aacaagaaac cgggttggtt
     1381 aaccatgcta attcaggaag tccgctataa tccggataaa atagcaaatg gccaggaatc
     1441 cacaaccggg cagttgacac tgaacattac cggggttaat cctgccctga aaggacaagt
     1501 cgtatatgct ctgtatacga gcgaccccat tatgccggat aacatggccc attctggggc
     1561 agttaacgca acggcccaaa cagttcctct tttcatcaac aacctgaccc atggagaata
     1621 cgccctcttt tgcttccatg acatc
//
LOCUS       DAOQXQ010000460         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_91872_length_1635_cov_3.203165, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000460 DAOQXQ010000000
VERSION     DAOQXQ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91872_length_1635_cov_3.203165"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKH81_07530"
     CDS             <1..896
                     /locus_tag="PKH81_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNQ97925.1"
                     /translation="AAPGDVEHIENPRARSIDMLFPIITLIVCAVVFFPLTTWLAAVD
                     GQKYLTLGAAVSAIPIGVAFNDTDASVALFYAAVFTLVITYVYYLARRLLTIKSASES
                     LRDGIASMVPALIVLTMAWTIGGVIKSSPADGGLGLAKYLSEVVVGGGFPVWLLPAVV
                     FILSAVISFSTGTSWGTFTIMIPIVMPIIVALAETKGLAGGALINACMISVSAVLSGA
                     IFGDHSSPISDTTILSSTGAGCPHLEHVSTQLPYALFVAGASLVGFAVGGVLLSDIAG
                     IISALCVFTLGLVFLPRLYKG"
     gene            complement(912..>1635)
                     /locus_tag="PKH81_07535"
     CDS             complement(912..>1635)
                     /locus_tag="PKH81_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNQ97926.1"
                     /translation="AGRRINLVISRGVIIDRIENYVGQNLDEVKMQLQAMFTSMATPL
                     IVITEPPLYRFSEEPAGTILEQKPAPDTPLAGPTKVELVVSRGPEGDRVTVPDITGRS
                     IREMYSIMEKTNLVYDFSLRNPEGSERAGTIVSQMPAGEARVNSYSRISTVLAVPVAN
                     PEGKVYGIFSETLPEYPYPFQIKLSAISPNGERSPLVSVKHPGGNFSVPYLLEEGTVL
                     VLTILNKEVGTREVHADTDESQ"
BASE COUNT          303 a          423 c          451 g          458 t
ORIGIN      
        1 gggcagcccc gggggatgtt gaacacatcg aaaatccccg cgcccgatcg attgatatgc
       61 ttttccctat catcacctta attgtctgcg cggttgtctt ttttcccctt accacctggc
      121 ttgccgcagt tgatggtcag aaatatctaa cccttggcgc cgctgtgtcc gccatcccga
      181 tcggtgttgc ctttaacgac accgatgcct cggttgcact tttttatgcc gcggtattca
      241 cgcttgttat tacctatgtg tattatctgg cccgcaggct cttaaccata aaaagcgcga
      301 gcgagtccct tcgcgatggt atcgcctcca tggtacctgc tcttatcgtg ttaacaatgg
      361 catggactat tggcggggtt atcaagtctt ccccggcgga cggggggctt ggactggcga
      421 agtatctgtc tgaggtggtc gttggagggg gattcccggt gtggctcttg cccgcggtgg
      481 tatttattct ttcggcggta atctcgtttt ctaccgggac cagttgggga acctttacca
      541 ttatgatacc gattgtcatg ccgattatcg tggccctggc cgagactaag gggcttgccg
      601 gcggggcact gattaacgcc tgtatgatta gcgtgtcggc ggtgttaagc ggcgcaatct
      661 ttggcgatca ttcctcgcca atttccgata ccactattct ctcttcaact ggtgcggggt
      721 gtccgcattt ggagcacgtg agtacgcaac ttccctatgc actttttgtg gccggagcaa
      781 gtttggtcgg tttcgcagtg ggaggcgttc tcttgagcga catcgcaggt attatctccg
      841 ctctctgtgt ctttacgctt ggactggtgt tccttccccg gctctacaaa ggctgagccg
      901 ggcagggagc atcactgact ttcgtcagtg tccgcgtgaa cttcccttgt tcctacttcc
      961 ttgttaagga tcgtcaacac aaggacggtt ccttcttcca ggaggtaggg cacgctaaag
     1021 tttcctccgg gatgctttac gcttaccagg ggagatctct ctccatttgg gctgatagcc
     1081 gaaagcttga tctggaaggg ataggggtat tcgggcaggg tttccgagaa gatgccgtac
     1141 accttacctt ccggatttgc gacggggaca gccagcaccg tggagattcg gctatagctg
     1201 tttacgcggg cttctccggc aggcatctga gatacaatgg tacctgcgcg ctccgatcct
     1261 tcgggatttc ttaaagaaaa atcatacacc agattggttt tttccattat ggaatacatc
     1321 tcgcgaattg atcttccggt aatatcgggt acggttaccc gatctccttc ggggccacgg
     1381 cttacgacaa gttcaacctt ggtgggacct gcgagcgggg tgtccggggc gggcttttgc
     1441 tcaaggatgg ttccggcagg ctcttcgcta aagcggtaga gcgggggctc tgtaattaca
     1501 atgagcggtg ttgccatgga ggtaaacata gcctgaagct gcatctttac ctcgtccagg
     1561 ttttgaccga cgtagttttc aatgcgatcg atgataacac cgcggctgat aaccagattg
     1621 atccttcttc ccgcc
//
LOCUS       DAOQXQ010000461         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_91909_length_1635_cov_2.234810, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000461 DAOQXQ010000000
VERSION     DAOQXQ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91909_length_1635_cov_2.234810"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PKH81_07540"
     CDS             complement(<1..1035)
                     /locus_tag="PKH81_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97927.1"
                     /translation="MKDIHTIQAAIEHCNASSETNFSRLAEAFPQLLSVVADTSSNTS
                     LPDLHNLLAALLSGFTDTSTGNDGDEDFFTRYNAETSSLFDTLNERMNALEHIHGRVE
                     GIRKDSEELELISLNAMVISIKSGEKGRAFSCITENLKRLSAQLISLSNNLILEEKRL
                     LEKNADFRSRFQSVSRQESLDGFRENALAGKAIVPALTEGTIALRTMAERATEVKKPI
                     RDAMSGIQLQDIIRQSNDQIILALKEVSPVTSFSSAEEALDHASVNRELLEICKTISE
                     DVARNLESSISIFTSNWATVNRILDEVDSLRRDFSTRYLEHTESATSLPNLLARITAS
                     SRCRCRPLKMP"
     gene            1155..>1635
                     /locus_tag="PKH81_07545"
     CDS             1155..>1635
                     /locus_tag="PKH81_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ97928.1"
                     /translation="MKQSTKKIVVILAVIALVCLMVYNFFTGNYNQMVSLDQGVKSQW
                     GQVENVYQRRFDLIPNLVATVKGYAAHESSVLTEVTEARSRAGGVMNISDEVLNDPEA
                     FARFQRAQGEISGALQRLLMVTENYPDLKANQNFIALQDQLEGTENRISVERKRFNER
                     "
BASE COUNT          457 a          359 c          407 g          412 t
ORIGIN      
        1 cggcattttc agcggcctgc aacggcagcg tgaagaggcc gttatccggg ccaaaagatt
       61 cggcagggat gtggcggatt cggtgtgttc caaataccgt gtggaaaaat cccttcgcag
      121 agagtcaacc tcgtcaagga tacgattaac cgtagcccaa ttagaggtaa aaatggaaat
      181 cgaggattcc aggtttcgtg ccacatcctc ggaaatggtt ttgcatatct ccaaaagctc
      241 ccggttcacc gaggcatgat ccaaagcttc ttcggcggag gaaaaagaag taaccggaga
      301 gacttcctta agggcaagaa taatttgatc attagattga cgaataatgt cctgaagctg
      361 tataccgctc atggcatccc gtatgggttt tttaacctct gtggcccgtt ctgccatagt
      421 gcgtaaggca atagtgccct cggtaagagc tggtacaatc gccttgccag caagggcatt
      481 ttccctgaat ccatcaagag attcctgtct ggagactgac tgaaaacgac tcctgaaatc
      541 cgcgtttttt tccaaaagcc gcttttcttc cagaataagg ttgttcgaaa gactgataag
      601 ttgggcagac aggcgtttta gattctctgt aatacaggaa aaggcccgtc ccttctcgcc
      661 ggatttgatt gaaattacca ttgcgttcag ggaaataagc tccagttcct ctgaatcctt
      721 gcgtattccc tctacacgtc catgtatatg ctcaagggca ttcatgcgct cgttgagcgt
      781 atcaaaaaga gacgaggttt ccgcattata gcgggtaaaa aaatcttcat ccccatcgtt
      841 tcctgtagac gtatcggtaa aaccggaaag aagagcggcc agcaagttat gaaggtcggg
      901 aagagaggta tttgaagaag tgtcggctac aaccgaaagc aattgcggaa aggcctcggc
      961 aagacgggag aaattcgttt ctgaggaggc gttacaatgc tcgattgctg cctgtattgt
     1021 atgaatgtct ttcacagtat acacaatcat aacggactgg ctacaggctg tacagaggaa
     1081 gaataaaaaa acacttcttg acccattgag agctttcccg taccttttat ctacagctac
     1141 ttttggagga aggaatgaag caatcgacaa agaaaatcgt tgtgattttg gcagttatcg
     1201 ctcttgtgtg ccttatggtg tataactttt ttacgggaaa ctacaatcaa atggtttcac
     1261 ttgatcaggg ggtaaaaagc caatgggggc aggttgagaa tgtctatcaa cggcgttttg
     1321 accttattcc caatctggtt gcaacagtaa agggatatgc cgctcatgaa tcgtcggtgt
     1381 taaccgaggt taccgaggca agaagccgcg caggcggcgt tatgaatatc tctgacgagg
     1441 tactcaacga ccctgaggcg tttgcccgtt ttcaacgcgc acagggagaa atttctggcg
     1501 cacttcagcg gcttttaatg gtaacagaga actacccaga ccttaaagca aaccaaaact
     1561 ttattgcact tcaggaccag ctggaaggca ccgaaaaccg tatcagtgta gagcgcaagc
     1621 gttttaatga acgag
//
LOCUS       DAOQXQ010000462         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_91966_length_1634_cov_2.996833, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000462 DAOQXQ010000000
VERSION     DAOQXQ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91966_length_1634_cov_2.996833"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1634
                     /locus_tag="PKH81_07550"
     CDS             <1..>1634
                     /locus_tag="PKH81_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNQ97929.1"
                     /translation="RCGVFAKTDIQPLINEGARREDIAASIYQAVVSQTISGLACGKP
                     IRGNVAFLGGPLHFMDQLRHRFIETLKLKDDEIFVPEHSQLFVAAGAAYSARLPGEGE
                     EEEEKEALRLISPAALREDLKKLVGAEMNEIQRLEPLFANEKELEEFRIRHAAEMAAS
                     ADLDSARGPVFLGLDAGSTTTKAVLIDDAGRILWRFYDTNGGNPVDLAVRVLKDLYRR
                     LPSSVRIMRSCSTGYGEALFQAALGVDAGEVETIAHYRAADFFVPGVEFILDIGGQDM
                     KCLRMKNGAITSIQLNEACSSGCGSFLDNFARSLGMDINTFSNRALLADKPVDLGSRC
                     TVFMNSRVKQAQKEGASVGDISAGLSYSVIKNALFKVIKLRDASAIGEKVVVQGGTFN
                     NDAVLRAFEKISGRHVFRPDVAGLMGAYGAALIALDQWKGAGSPGDARTGLATPEQLE
                     HFAVELELRRCGKCSNNCLLTINTFTAGVVTTGPVEQMVAPPRRFITGNRCERGLEID
                     LSVKPAVGDADHAGNTGAISIAENHDEPIGRGKPEK"
BASE COUNT          358 a          414 c          451 g          411 t
ORIGIN      
        1 ccggtgtgga gtgtttgcca agacagatat ccagcctctt attaacgagg gtgcccggag
       61 ggaagacatt gcggccagta tttatcaggc cgtagtaagt caaaccatct ccggcctggc
      121 atgcggaaaa cccattcgcg gtaatgtcgc ctttcttggc ggaccgcttc attttatgga
      181 tcagcttcgc caccgcttta tcgaaaccct taaacttaaa gacgacgaga tttttgttcc
      241 ggagcactcc caactctttg tagccgccgg ggcagcttat tccgctcgcc tccccgggga
      301 gggagaagaa gaagaggaaa aagaagcgct gcgtcttatc agtcccgcag cccttcgcga
      361 ggatcttaaa aaactggtcg gcgccgagat gaacgagatc caacgccttg aacctctttt
      421 tgcgaacgaa aaagagcttg aagaatttcg cattcgtcat gcggcagaga tggccgcttc
      481 tgcggacttg gatagtgcac gcgggccggt ctttttaggc ctggacgcgg gctcaactac
      541 taccaaggct gtgttaatcg atgacgcggg aagaatcctc tggcgtttct acgatacaaa
      601 cggcggtaat ccggttgatt tggccgttcg tgtccttaag gatctctatc gccgcctgcc
      661 ttcttcggtt cgtattatgc gctcctgttc taccggctat ggcgaggctc tgttccaggc
      721 tgccctgggg gtagacgcgg gagaagtaga aacaatagct cactaccgtg ccgcggattt
      781 ctttgtcccc ggtgttgagt ttatccttga tattggcggt caggatatga agtgtttgcg
      841 catgaaaaac ggcgcgatta cctcaatcca gcttaacgag gcctgctctt caggatgcgg
      901 tagcttcctg gacaactttg cccgatccct cgggatggat attaacacct tctccaatcg
      961 tgcccttttg gcggacaaac cagttgatct gggaagccgc tgtactgtct ttatgaatag
     1021 tcgggttaag caggcgcaaa aagaaggagc ttccgttgga gatatctccg cgggtctttc
     1081 gtattcggtt atcaagaacg ctctttttaa ggttatcaaa ctacgcgatg cttctgccat
     1141 tggcgagaag gttgtggttc aaggcggtac ctttaacaac gacgcggttt tacgcgcgtt
     1201 tgaaaagatt tccggcaggc acgtattccg tccggatgtt gccggcttga tgggtgccta
     1261 tggagcagcc cttattgcac tggatcaatg gaagggcgcg ggctctcctg gggatgcccg
     1321 aacgggactt gcaactcccg aacagcttga acattttgcc gttgagcttg aattgcgccg
     1381 atgtggcaag tgttccaata actgtttact caccatcaat acctttaccg ccggggttgt
     1441 taccacagga cccgtagaac agatggtcgc tcctccccgc aggtttatta cgggcaaccg
     1501 ctgtgagcgg ggcctggaga ttgatctttc ggtaaaaccc gctgttgggg atgccgatca
     1561 tgccggaaat accggcgcta tctctattgc ggaaaaccat gatgagccga ttggtcgggg
     1621 aaagcccgag aaac
//
LOCUS       DAOQXQ010000463         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_92052_length_1633_cov_3.924588, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000463 DAOQXQ010000000
VERSION     DAOQXQ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92052_length_1633_cov_3.924588"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PKH81_07555"
     CDS             complement(<1..155)
                     /locus_tag="PKH81_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ97930.1"
                     /translation="MFKKLNDKDRKRLKRKIHIRKRLFGTEARPRMTVYRSNSNLSVQ
                     VINDDAGV"
     gene            complement(168..707)
                     /gene="rplF"
                     /locus_tag="PKH81_07560"
     CDS             complement(168..707)
                     /gene="rplF"
                     /locus_tag="PKH81_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ97931.1"
                     /translation="MSRVGKLPVAIPAGVKVTVTNQTVVVEGPKGKLSQDFQPVVDIK
                     VEGDVANVTRKDDSKVSRSCHGLYRNLLNNMVVGVTSGFTKTLVINGVGYRAEVQGNL
                     LVMNLGYSTDFIAGIPEGLTVTADAQGRIVITGVRKDQVGEFAAQVRKLRGPEPYKGK
                     GIRYETEVIKRKVGKSGVK"
     gene            complement(722..1120)
                     /gene="rpsH"
                     /locus_tag="PKH81_07565"
     CDS             complement(722..1120)
                     /gene="rpsH"
                     /locus_tag="PKH81_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNQ97932.1"
                     /translation="MSVSDPIADMLTKVRNAAMARHEKVDIPASKLKLEIVKILKTEG
                     FIKNFKKVNQEGSNCIRVFLKYDDKNLPVIHGIEKLSTPGRRMYAGYKDLPRVYNGYG
                     TVIVSTSLGVTTGKKAQEKQVGGELICTVW"
     gene            complement(1136..1321)
                     /locus_tag="PKH81_07570"
     CDS             complement(1136..1321)
                     /locus_tag="PKH81_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNQ97933.1"
                     /translation="MATTAMINKANATPKYSTRKYNRCRVCGRPRGYLRKYAMCRVCF
                     RKLASEGLIPGVTKSSW"
     gene            complement(1333..>1633)
                     /gene="rplE"
                     /locus_tag="PKH81_07575"
     CDS             complement(1333..>1633)
                     /gene="rplE"
                     /locus_tag="PKH81_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881648.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ97934.1"
                     /translation="QGNEVGVMVTLRGAKMYEFFDRFVNVALPRVKDFRGINPNGFDG
                     HGNYSLGITEQIIFPEIDFDKIERVAGLNINVVTSAKTDAEAKALLSKFGMPFRK"
BASE COUNT          386 a          433 c          358 g          456 t
ORIGIN      
        1 accccagcgt cgtcgttaat tacctgaacg gaaagattgc tattgctgcg gtataccgtc
       61 atgcggggac gagcctcggt tccgaaaagc cgcttgcgta tgtgaatctt tctcttaagt
      121 ctcttgcgat ccttatcatt aagtttcttg aacatttcgc cttaccctta tttgacgccg
      181 gatttaccga ctttccgctt gataacttcg gtctcgtaac gaataccctt acccttataa
      241 ggctcgggtc cacgaagttt gcgtacctgt gcggcgaact cgccaacctg atccttgcgg
      301 actccggtaa tgacgatcct accctgggcg tcagccgtta cggttaaacc ctcgggaata
      361 cctgcgataa agtcggttga ataaccgaga ttcatcacca acaggttgcc ctggacctct
      421 gcacgatacc caacaccgtt aataacgagt gttttggtaa agccggaggt tacgccgacg
      481 accatattat taagaagatt gcgatacaaa ccatggcacg agcgggagac ctttgagtca
      541 tccttacggg ttacgtttgc aacgtctcct tctactttga tatcgactac cggctgaaaa
      601 tcctgggaaa gctttccctt tggaccttca acaacgacag tctgattggt aacagttacc
      661 ttaactcccg ccggtatcgc aacaggaagt ttaccaactc ttgacatctg ttactcctgt
      721 cttaccagac ggtacagatc agctcaccgc cgacctgctt ctcctgggct tttttaccag
      781 tggtaacgcc aagagaggtt gatacaataa cggtaccata cccgttataa acacggggta
      841 ggtccttata tccagcatac atccgtcttc ctggtgttga aagtttttca ataccatgga
      901 ttacgggaag gttcttgtca tcgtacttga gaaatacgcg aatgcagtta ctgccttcct
      961 gattaacctt cttaaagttt ttaataaaac cttcggtctt caggatcttc acgatctcga
     1021 gttttaactt ggaggcggga atgtccactt tttcgtggcg ggccatcgcc gcattccgga
     1081 ccttggtcaa catgtctgct atgggatctg aaacgctcat ttaatgtcct ccctcctacc
     1141 agctcgactt ggtgacgccc ggaatcaggc cttcacttgc taacttccga aaacatacac
     1201 gacacatcgc gtatttccgg agatatccac gggggcgacc gcataccctg caccgattgt
     1261 attttcgggt agaatacttc ggggtggcgt tcgccttgtt aatcatcgct gttgtagcca
     1321 tgaactcctc acttactttc tgaagggcat gccaaacttg ctcaaaagcg ctttggcttc
     1381 ggcatctgtt ttggcactgg taaccacgtt gatattcaaa ccggcgactc tttcgatttt
     1441 gtcgaagtcg atctccggga agataatctg ttcggtgata cccaatgaat agtttccgtg
     1501 accatcaaat ccgttaggat tgataccgcg gaaatccttg acgcgaggaa gtgctacgtt
     1561 tacaaagcga tcgaagaact cgtacatctt tgcaccgcgc agcgttacca taacgcccac
     1621 ttcatttccc tga
//
LOCUS       DAOQXQ010000464         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_92745_length_1626_cov_2.461489, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000464 DAOQXQ010000000
VERSION     DAOQXQ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92745_length_1626_cov_2.461489"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKH81_07580"
     CDS             <1..844
                     /locus_tag="PKH81_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ97935.1"
                     /translation="ELYGSIPTAITSFTEVVGRKALVILSDGEHLSHPDSRLDFTIKQ
                     GIDAAIREGVTCYVVNFGTSRDNTLPDLADASGGVVFNAASSQDLSDIYERIRSSILD
                     EWAIRYIASMTAGDRRYVRVSSPSNKIPSGPARVYYSGGVLGTGMARPSWFHLFAILL
                     PLAAWLALVFCKLERETTDAEIRLLYGASSGQTRAFTLTSAQTVIGGAASSDITIAGN
                     PSLKDNAATVVYDKTRARYTIVAESGVTVNNKPVKNRVLEDGDVINMAGTVVVFDDRK
                     KPVK"
     gene            complement(1062..>1626)
                     /locus_tag="PKH81_07585"
     CDS             complement(1062..>1626)
                     /locus_tag="PKH81_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97936.1"
                     /translation="ETIIRVAYDRLTPKPMSIRYQAVDAVSAKDITPASRLSVLEKGS
                     WVPLDTIKPEMLQTGTIRKFRIEADGYYEEIYSLKIAHSQDELFINARLVPLSGTLSI
                     KSNSSRVRITVNGSTDVILGGMEMKRESLASWRGEAASWKLPSGQYTIKVYSSKGEGT
                     LAVSVEAGKTVRIVATEKKGAIILQKE"
BASE COUNT          378 a          396 c          389 g          463 t
ORIGIN      
        1 cgagttatat ggttccattc ccaccgccat cacctctttt accgaggtgg tcggacgtaa
       61 ggcccttgtt atcttgtccg atggcgagca tctttctcat cctgattcac gccttgattt
      121 taccatcaag caaggtatag atgccgcgat tcgcgaaggg gttacctgct atgtggttaa
      181 ttttggaacc tcccgcgata atacccttcc ggatctggca gatgcatcgg gtggcgtggt
      241 gtttaatgcc gcaagctcac aggatctaag cgatatctat gagcgaatca gatcgagtat
      301 cctggatgaa tgggcgatcc gttatatcgc atccatgacc gcaggagaca ggcgctacgt
      361 tcgtgtatca agcccttcca ataagattcc ttccggaccg gcgagagtgt attattcagg
      421 tggtgtattg ggtaccggaa tggctcgccc ttcatggttt catctttttg cgattctgct
      481 tccactggcg gcatggttag cccttgtgtt ttgtaagctt gagcgcgaaa caactgatgc
      541 ggaaatcaga cttttgtatg gagcctcatc gggtcaaacc agggccttta cgcttaccag
      601 tgcgcaaaca gtcataggtg gcgctgcctc ttctgatata accattgccg gaaacccgtc
      661 ccttaaggac aatgccgcga ctgtcgtata tgataaaacc cgggcacggt acacgattgt
      721 cgctgaatcc ggggtaacgg taaacaataa gccggtaaaa aacagggtcc tggaagatgg
      781 cgatgttatc aatatggctg gtacggtggt agtctttgat gaccgtaaga aacccgtaaa
      841 atgagtctgt aaggacctgt attttctgct atttgtcgct ttgggctgca aatagcagaa
      901 aattgtaaca ataatgaaaa aatctccaaa aaaatggaat tatctattga ttctttttcc
      961 tctatcgagt aatttatcaa tgaaacctgc caccgctctt cagcatgtga tgcttccaag
     1021 ttctacttgc cgaatacctc cgattggcag gttttatttt tttattcctt ctgcaagata
     1081 atcgcgccct tcttctccgt tgccacaata cgaactgttt tccccgcctc tacagacact
     1141 gcgagcgttc cttcaccctt tgatgaatag accttgattg tgtactgtcc cgaaggcagc
     1201 ttccaggaag ccgcttcccc acgccaggag gccaggcttt ctcgtttcat ctccattccg
     1261 ccaagaatga cgtcggtgga cccattgacg gttattcgca cccgggaaga attgctcttt
     1321 atgctcaggg ttccgctcag gggcaccagg cgggcgttaa taaagagctc atcctgtgaa
     1381 tgggcaatct ttagggaata gatctcttca taataaccat cagcttctat acggaatttt
     1441 cggatagtgc cggtttgcag catttctggt ttgatggtgt ccaaaggtac ccagcttcct
     1501 ttttccagaa ccgacaatcg cgaggcaggg gttatatcct ttgcgcttac cgcatcaacg
     1561 gcctgatagc gtatgctcat cggttttggt gtaaggcggt cataggctac tcgaatgatg
     1621 gtttct
//
LOCUS       DAOQXQ010000465         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_92799_length_1625_cov_4.219745, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000465 DAOQXQ010000000
VERSION     DAOQXQ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92799_length_1625_cov_4.219745"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PKH81_07590"
     CDS             complement(<1..1351)
                     /locus_tag="PKH81_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ97937.1"
                     /translation="MQTALLDSYAMLPLAASLLVQGVVGLFLFRIAKHSHERAPEAIS
                     FKLPAISIAVLCLFTLATLVFPSLLVLSSGTLLSSIFIAIFLIVLSLTLPAQQEQPEL
                     VQPIPDSDSIQEEETETEDPLVATGQQFISRLSETLTGEVNIVRLLDYINDTLIEQTH
                     ADGGIVFLADDFEDSIAAKSFKGSFPPPYKLPQDLPHKPVRVETNFRYCQFNLGDNIF
                     GEVAASGKPVLIESGTMDDRIFVNGPEEFLKPGSYIVVPLMVKDRVVGVAGLARLPEN
                     APFNGQEFRIAEVLSEYAGASINNVYNVQEILEHADLEREASIASKIQKTLQPKRLPE
                     LPEVGFGSLFNATKGVCGDYYDVILARRDRIVVTIADVAGKGIQSSMVMIMLRSILHL
                     VTNTTKSAATVLDWVNKGITGKIDMDHYATLSYLSYCPENHTIEYASAGHQPLLVWRS
                     "
     gene            complement(1385..>1625)
                     /locus_tag="PKH81_07595"
     CDS             complement(1385..>1625)
                     /locus_tag="PKH81_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNQ97938.1"
                     /translation="VYLPVMAVILKRKLVRKFRNRTPSDLYVWIVKQWDELKQKYGSD
                     FSLDQATDTLEIAGERRQNRFFQALRSVFKRFIQT"
BASE COUNT          455 a          349 c          394 g          427 t
ORIGIN      
        1 gtgatctcca gactaaaaga ggctggtgac ctgcgctggc gtattcgatg gtgtgattct
       61 cgggacagta actgagatac gaaagggtag catagtgatc catatcaatc ttgccggtaa
      121 tgcccttatt tacccaatca aggacagtag cagcagattt agtggtgttg gttaccagat
      181 gtaagataga cctgagcata atcataacca tgctggactg tatgcctttg ccggcaacat
      241 ctgcgatggt aacaacaatg cggtcgcgac gggcaagaat cacatcataa taatctccac
      301 acacaccctt tgttgcgtta aacaatgaac caaagccaac ttctggaagc tcaggtaggc
      361 gcttgggttg cagtgttttt tggattttac ttgcaatact tgcttcgcgt tcaaggtctg
      421 cgtgttcaag aatttcttga acattataga cgttattaat cgacgcacct gcgtattcag
      481 agagaacttc agcgattctg aactcttgtc cattaaaagg tgcgttctcc ggtaaacggg
      541 caagaccggc gactcctacg acacggtctt taaccatgag cgggacaacg atataactac
      601 caggcttaag aaattcttcc ggaccgttaa caaaaatacg gtcatccatt gtaccgcttt
      661 cgattaatac cggtttgcct gaggcggcaa cttctccaaa gatattatct ccgagattga
      721 attggcagta tcggaaattc gtttctactc gcactggttt gtgcggtaaa tcttggggaa
      781 gcttatacgg aggagggaag ctacccttga aggattttgc tgcaatactg tcttcaaaat
      841 catcagcaag gaaaacaata ccaccatccg catgggtttg ctcaattaag gtgtcgttga
      901 tgtagtcaag aaggcgaacg atatttactt ccccggtaag ggtttccgaa agtcgggata
      961 taaactgttg accagttgcg acaagaggat cttcggtctc tgtctcttct tcctggatag
     1021 aatcactgtc cggtatgggt tgaaccaatt ccggctgttc ttgttgagcg ggaagggtaa
     1081 gggacagaac aattaaaaaa atggcaatga atatggatga gaggagagtc cctgaggata
     1141 gcaccaataa gcttggaaaa accaaggttg ccaaggtaaa aagacaaaga acagcgatcg
     1201 aaatagccgg aagtttgaat gaaatggctt cgggtgcccg ctcatgagag tgtttggcta
     1261 ttctaaacaa gaacagacca actaccccct gaaccagcag actggcagcc aacggcagca
     1321 tggcgtatga atcaagtagt gctgtttgca ttatgcctca gtgtaccatt gaaatttcgg
     1381 gtcgtcaagt ttgaatgaac cttttgaaaa ccgaccgcag cgcttgaaaa aagcggtttt
     1441 gacgcctttc cccggctatt tccagggtat cggtggcttg atctagagag aagtcgcttc
     1501 cgtatttctg cttaagctcg tcccattgtt taacaatcca cacatacaga tcgcttggtg
     1561 tgcggtttct aaacttgcgg actagtttac gtttaaggat tacggccata accggaagat
     1621 agaca
//
LOCUS       DAOQXQ010000466         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_92821_length_1625_cov_3.167516, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000466 DAOQXQ010000000
VERSION     DAOQXQ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92821_length_1625_cov_3.167516"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKH81_07600"
     CDS             complement(<1..643)
                     /locus_tag="PKH81_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97939.1"
                     /translation="MNYQRWPIVTLILCFVCIIGYIFINDANWKMDSAFRDVQVNTYR
                     AYSAWHRYIGTLKGLTLTNEGFGTSLDTARDLQRDTERLLIELSKKSETMNPDIAQTI
                     EAFSASIQASITLGQELIENGYLLLAQPDLPLIYSEGRIGLSSLSGKDVTEILGPLSS
                     YQYYQLIRRLKGLNTLFDQLYSSRMDGILTKIENQSERLRRDFFLLRLIVLGIT"
     gene            complement(644..>1625)
                     /locus_tag="PKH81_07605"
     CDS             complement(644..>1625)
                     /locus_tag="PKH81_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97940.1"
                     /translation="SALCIDNAEMIEDLAPALQAILARHTVTIILAGRNSRTLETLID
                     RHFSTEGSISRETITVYPLSYPEFIQATGRKESRETLDLYCRTGGLPQSLLIDPTSPD
                     SRELHRIRSNSFLLTELIEVHAIRNPVYIRHILSLVARNTGERLSARMVCAVFSRMRL
                     TISPQSALDYLAFCQDSGILIATPLFDIAKNRIVENCESWYFADAGLRSSFCPLDNNR
                     DLSRAEENLALLRLISDGWKVMRGRLGSGSASKEEITFVCLRGDQKAYVEILGSRTSG
                     ADRLKKTKVLLCVPDAWPRYLVDPAGTEDSADGINRLSIRELLLEGLKGS"
BASE COUNT          404 a          375 c          407 g          439 t
ORIGIN      
        1 aggttattcc aaggacaatg agcctgagca ggaaaaaatc tcgtctcagt cgttcggatt
       61 ggttttctat tttggtaagg atgccatcca tgcgggagga ataaagttga tcaaaaaggg
      121 tgttaagtcc tttgagtctt ctgatcaact gataatactg ataggaggac agcgggccca
      181 gtatctcggt tacatccttg cctgatagac ttgataggcc aattcttcct tcgctatata
      241 tcaggggcag gtctggctgt gcaagcagga ggtatccatt ctcgatcagc tcctgcccaa
      301 gggtgatgct tgcctgtatg ctcgcgctaa aggcttctat tgtttgcgca atatccggat
      361 tcatggtttc gctttttttc gaaagttcga taagcaagcg ctctgtatcg cgctgtaaat
      421 cccgggcggt atcaaggctt gttccaaagc cttcgttggt aagggttagt cctttcagtg
      481 tgccgatata tcggtgccat gctgaatagg cgcggtatgt atttacctgc acatcgcgga
      541 aggcgctgtc cattttccag tttgcgtcgt taataaaaat gtagccgatt atacagacaa
      601 agcagagaat aagggttacg attggccagc gctggtaatt catctagctc ccttttaatc
      661 cttccagtaa taactcgcga attgatagcc ggtttattcc atcggcggag tcttcggtac
      721 ctgccgggtc cacaagatag cgaggccacg catcaggtac acaaagcaat acctttgttt
      781 ttttcaggcg atctgctccg cttgtgcggc ttcccagaat ctccacgtag gctttctgat
      841 cgcctcgcaa gcaaacaaac gttatctctt ccttgctggc ggaccctgaa ccgagtcttc
      901 ctcgcattac cttccagcca tcactgataa ggcgtaaaag agcaaggttt tcctcggccc
      961 gggaaagatc gcggttattg tccaaaggac aaaaggacga gcgcagtcct gcatcggcaa
     1021 agtaccaaga ttcacaattc tctacgatgc gattttttgc tatatcaaac aggggtgtcg
     1081 ctattagtat accagagtcc tgacaaaacg ccagataatc cagtgcggat tgcggggaaa
     1141 ttgtcagacg catacgggaa aaaacggcac aaaccattct tgcactcagt cgctctccgg
     1201 tatttcgtgc aaccagcgaa aggatatggc gaatatagac aggatttctg attgcatgaa
     1261 cttcaatcaa ttcggttaac aagaatgaat ttgagcggat gcgatgcaac tctcgtgaat
     1321 ccggactggt cggatcgatg agaaggcttt gggggaggcc tccagttcgg caatacagat
     1381 caagggtctc tcttgactct ttacgaccgg tagcctggat aaactcagga tatgagaggg
     1441 ggtatacggt gattgtttcc cgactgatac tgccttccgt gctaaaatgc cgatcgatca
     1501 gtgtttcaag ggttctggaa ttccttccgg caaggataat cgttacggta tgtcgtgcca
     1561 gtattgcctg tagggcagga gcaaggtctt cgatcatctc tgcgttatca atacataagg
     1621 ccgac
//
LOCUS       DAOQXQ010000467         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_93030_length_1623_cov_2.234694, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000467 DAOQXQ010000000
VERSION     DAOQXQ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93030_length_1623_cov_2.234694"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKH81_07610"
     CDS             <1..368
                     /locus_tag="PKH81_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNQ97941.1"
                     /translation="CLSGRCGSCTVLLDGKPVASCIVPVFLVRHGSVETLESFSKTEA
                     YRDIKDGFAEAGVEMCGFCNAGKILHAHYLLEVHPRPTVEEIQACFTASICRCTSMED
                     LIEGVKKAAAIRRRRSNRA"
     gene            361..1200
                     /locus_tag="PKH81_07615"
     CDS             361..1200
                     /locus_tag="PKH81_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNQ97942.1"
                     /translation="MLNKRTTVYYAASIAEAQTILKNIAGISLCAGCTEIARRQTGRN
                     LHLPAHVLSLSRVPELCTISKTERYIEFGSSVTLSSMLELGKKNLSEVLFEAIQTIAN
                     PSIRTLATLGGNIAAKGHRLTTFAPLLALDARLEVRSPTEALWVPMTRHFSNTGKETP
                     VTGEFISKIRVPNDNWDLQIFKRVGRPGVITDSTSSFAFLVKSQKKLLADIRIAWAGK
                     FFFRQREFENLIIGRSLPLSERDIINLMDKSSLFFDPGLFPPSYERSCFFNLLEDSLR
                     LLT"
     gene            complement(1207..>1623)
                     /locus_tag="PKH81_07620"
     CDS             complement(1207..>1623)
                     /locus_tag="PKH81_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNQ97943.1"
                     /translation="HVGLGTFLPVRAERIEDHVMHEEHYQVSAEAAAMVTKAKREGRP
                     VVAIGTTSIRVLESAWNDEKQELTAGEGSTNIFIYPGYRFKAVNRVFTNFHTSESTLL
                     MLVSAFAGRDVIMSAYQHAIEKRYRFFSYGDSMLIV"
BASE COUNT          411 a          437 c          374 g          401 t
ORIGIN      
        1 gttgcctgtc cggtagatgc ggttcatgta cggtccttct tgacggtaaa cccgttgcct
       61 cgtgcatcgt gcctgttttt ttggttcgcc acggatcggt ggaaaccctt gagtcttttt
      121 ccaaaaccga agcataccgg gatattaagg acgggttcgc cgaagcaggg gtcgagatgt
      181 gcggtttttg caacgccgga aaaattcttc acgctcatta cctattagaa gtgcatcccc
      241 gtccaaccgt agaagaaata caggcatgct ttaccgcaag catatgtcgc tgtaccagta
      301 tggaagattt gatagagggc gttaaaaagg ccgcggcaat acgcagaaga aggagtaacc
      361 gtgcttaata aacgaaccac cgtctactac gcggcctcca tagccgaagc gcaaacaatt
      421 ttgaagaaca tagccggcat ttccctgtgc gcgggctgca ccgagattgc tcgacggcaa
      481 acaggaagaa accttcactt accggctcac gtactttccc tttcccgagt acccgaatta
      541 tgtaccatca gtaaaacgga acgctatata gagttcggct cttcagttac cctatcgtcc
      601 atgttggagt tgggcaaaaa gaacctgagc gaagtactct ttgaagcaat acaaacaatt
      661 gccaatccct ccatccgaac ccttgcaacc cttggaggta acatagcggc aaagggccat
      721 cggctgacca cctttgcccc ccttttagcc ctggacgcgc gccttgaggt tcgtagcccc
      781 accgaagcac tctgggttcc catgacgagg catttctcta acaccggaaa ggaaacaccg
      841 gtgactggcg agtttatcag caagatacgg gtgcccaacg ataactggga cctacagatt
      901 tttaagcgag tgggaaggcc aggagtcata accgattcaa cctcctcctt tgccttttta
      961 gtcaaatcgc aaaagaagct gcttgccgac attcgcattg cctgggcagg aaaattcttt
     1021 tttagacagc gagaatttga aaacctgatc attggacgat ctctgccctt aagcgagcga
     1081 gacatcatca acttgatgga caaatcttcc ctgttttttg atccagggct ctttccccca
     1141 tcctatgagc gcagctgttt ttttaatctg ctggaagact cactacggct acttacctaa
     1201 ggccttttac acaataagca ttgaatcccc atacgagaaa aagcggtatc gcttttctat
     1261 ggcatgctgg tacgcactca taattacatc gcggccggca aacgcggaaa caagcatcaa
     1321 gagcgtggac tcgctggtat ggaagttagt gaacaccctg ttcaccgcct taaagcgata
     1381 gccgggataa ataaagatat tcgttgaacc ttcgcctgcg gtcagttctt gcttctcgtc
     1441 attccaggcc gattcaagaa cgcgaatact tgtagtgcca atcgccacca ccggccgccc
     1501 ttcccttttt gcctttgtca ccattgctgc cgcctcggcg cttacctgat agtgctcttc
     1561 gtgcatcaca tgatcttcta tacgttccgc tcgtaccgga agaaaggtgc caagacctac
     1621 atg
//
LOCUS       DAOQXQ010000468         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_93694_length_1616_cov_3.049327, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000468 DAOQXQ010000000
VERSION     DAOQXQ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93694_length_1616_cov_3.049327"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..559)
                     /locus_tag="PKH81_07625"
     CDS             complement(254..559)
                     /locus_tag="PKH81_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97944.1"
                     /translation="MVKAAVVHADMLYMIQLDDLIILLQAVLSSPEVIIVTVAVLLYM
                     SIINWIVRYRKRPPRPARKKAPVAVAEKKSEVQEDEDERDGPDDEEDEAPPVKKKRR"
     gene            587..1225
                     /gene="hisH"
                     /locus_tag="PKH81_07630"
     CDS             587..1225
                     /gene="hisH"
                     /locus_tag="PKH81_07630"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNQ97945.1"
                     /translation="MTGIIDYKAGNIRSVELALRTLGVNYRISDTPEKLEGADRLIFP
                     GVGEAKYAMKNLKERGFDAFLHQWASSGKPLLGVCLGSQIIFEYSEEGDTPCLALVPG
                     AVRRFPDHFREHGLKIPHMGWNNVTFTNGGSRLFDGIQEGTDFYFVHSYYVDPADRSI
                     ISATVDYGMEVPCGIRHGAIEAFQFHPEKSAMGGLRILANFTAADRQKGPLC"
     gene            1219..>1616
                     /locus_tag="PKH81_07635"
     CDS             1219..>1616
                     /locus_tag="PKH81_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNQ97946.1"
                     /translation="MLKKRIIVCLDVKDGRTTKGVQFLDNVDIGDPVEMALEYYRQGV
                     DELVFYDIMASARDRGPTLDLIARVAEAIHIPFCVGGGITTVEDIRSTILSGAEKISL
                     NSQAVKNPSLIQEGAAIFGNQCIVLGMDAKR"
BASE COUNT          376 a          418 c          418 g          404 t
ORIGIN      
        1 gtttgcgatg gaaaagctga agtcttgccg actgaggacc gtcttgtcct tgattttacc
       61 cggctacatc cccgctcgta tagtcaaaac gatatggcgg aaattcttta cgccggggct
      121 aacgcacatg agcggatgag gggctggcaa tatagctgga gagtcagcga tgtacacgcc
      181 tcaatcgccg ctcttcttga agccggaaca ttgcagaaaa gccgctactg gccacagaag
      241 ggcagactct accttagcga cgctttttct ttaccggcgg ggcctcatct tcttcgtcgt
      301 cagggccgtc ccgctcatct tcgtcttcct ggacttcact ctttttttcg gcgacggcaa
      361 caggggcctt cttgcgggcg ggacgtggcg ggcgtttccg gtagcgaaca atccagttaa
      421 taatcgacat atacaacaat actgctacgg taacgattat tacctccgga gaggagagta
      481 ccgcctgcaa taatataata agatcatcca gttgtatcat atagagcata tcggcatgta
      541 ccacagccgc cttgaccact accctcgcca ttggtacgat acgcatatga cgggaataat
      601 tgactataaa gcaggtaata tccgcagcgt tgaacttgcc ttgcgtacgc ttggggttaa
      661 ctaccgcatt tccgacactc cggagaagct tgaaggggca gaccgcctta tctttccggg
      721 agttggcgag gccaagtatg ccatgaaaaa ccttaaggaa cgcggctttg acgccttttt
      781 gcaccagtgg gcctcatccg gcaagccctt gcttggggta tgccttggct cacaaatcat
      841 ttttgaatac tcggaggaag gggacacgcc ctgtcttgcc ctcgtccccg gtgcggtgcg
      901 ccgctttccc gaccacttcc gcgagcatgg ccttaaaatt cctcatatgg gctggaacaa
      961 tgtcaccttt accaatggtg gctctcgcct gtttgacgga attcaggaag gcacggattt
     1021 ttactttgtt cactcatatt acgttgatcc cgcagatcgc tccattatca gcgcgactgt
     1081 cgattacggt atggaggtcc cctgcggaat ccgccatggc gccattgaag cctttcagtt
     1141 tcatccagag aaatcggcta tgggcggctt acgaatcctt gcaaacttta ccgccgcaga
     1201 tcggcaaaag ggtcccttat gctaaaaaaa cgaattatcg tatgcctgga cgttaaggat
     1261 ggaaggacaa ccaagggcgt acaatttttg gataatgttg atattggcga tccggttgaa
     1321 atggctctgg agtattaccg ccagggtgtg gatgagctcg ttttttatga cattatggca
     1381 tcggcccgcg atcgcggtcc gacgcttgat ttgatcgccc gggtagccga ggcaatacac
     1441 atcccctttt gtgtgggcgg aggcattacc accgtagagg atattcgctc caccatcctc
     1501 tccggcgcgg aaaaaataag ccttaattca caggcggtaa aaaacccgtc gctgattcaa
     1561 gaaggcgcgg ctatttttgg caatcaatgc attgtgttgg ggatggacgc aaaacg
//
LOCUS       DAOQXQ010000469         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_94005_length_1613_cov_2.900513, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000469 DAOQXQ010000000
VERSION     DAOQXQ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94005_length_1613_cov_2.900513"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKH81_07640"
     CDS             <1..918
                     /locus_tag="PKH81_07640"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97947.1"
                     /translation="APIPQNRSYSSVFPINHPRRGEPVSFTEDFLKPFQRSLMQSLYR
                     KHKHYIFFAEGIPMGERVSWPIEDRVREDEGYFSVVDAFHWYDGVTLLSRRWRPWLTA
                     DGERGTAVLGKARVKKSIHTQLERMVLRAAADGVPGFLAEFGLPFNLNRGSAYQSGDY
                     SRHEAALASYYDGVDSNLLHSTIWNYTAGNTHTKGDGWNGEDLSVYCGETLSPRAVKG
                     FSRPYAMAVCGTPRRMSFDTKNAEFSLEWDGEAGETEVYVPDHWYPQGWQSLVVCGTA
                     RLEERADRQRLVIVSETAGFLSIRISRRS"
     gene            complement(915..>1613)
                     /locus_tag="PKH81_07645"
     CDS             complement(915..>1613)
                     /locus_tag="PKH81_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNQ97948.1"
                     /translation="LAVFIFALVVLAGKIIYTNFDSMAMSILGADEEVHLASIIAPDQ
                     FHLQAIKGGRATDPALTLYRSEAARADVLWFYTHITGDSEISRIILDNASKNNIPPAL
                     AFALAWEESRYQIRAVNKNATSIDRGLFQLNNKAFPKLTEREFFNPETNAHNGLTHLS
                     YCLDLSGNEVAALAMYNAGTTKVRNNNTPKRTLDYVSRIFSYREGLDKLFVQQVAARY
                     KIDEDNRVKAVVAR"
BASE COUNT          359 a          377 c          447 g          430 t
ORIGIN      
        1 gctccgatac cgcagaatcg aagttactct tcagtgtttc ccataaacca tccccgcagg
       61 ggcgagccgg tgtcctttac cgaggatttt cttaagccct tccagcgctc ccttatgcag
      121 tccctgtatc gtaaacataa acattatatt ttctttgccg agggaatacc gatgggagag
      181 cgagtgtctt ggcccataga ggatcgcgtt cgcgaggacg aaggctattt ttctgttgtt
      241 gacgcgtttc attggtacga tggcgttacc ttgctgtccc gtcgatggcg cccctggtta
      301 accgcagacg gggagcgtgg cactgcggta ttggggaagg cccgcgttaa aaaaagtatt
      361 catacacagc ttgagcgcat ggtcttacga gccgcagctg atggagttcc cggctttttg
      421 gcagagttcg gtcttccgtt taacctaaac cgcgggtcag catatcagag tggagactac
      481 tcgcggcacg aggcggcctt ggcatcttat tacgatggag tggattcaaa cttgctgcat
      541 tccacaattt ggaattatac cgcggggaat acccatacca aaggcgatgg atggaacgga
      601 gaggatctgt ctgtctattg tggtgaaacc ctctctccgc gagcggtaaa gggtttttcc
      661 cggccatacg caatggctgt ctgtggcact ccccggcgta tgagctttga tactaaaaat
      721 gcagagttta gccttgaatg ggatggagaa gcaggagaga cagaggtcta cgttccggat
      781 cactggtatc ctcagggatg gcagtctctg gtagtctgcg gcaccgcgcg attggaagag
      841 cgcgcagacc gtcagcgact agtgattgta agcgagactg cgggtttctt gtcgatacgg
      901 attagtcgac gctcttaacg agcaaccacc gctttgacgc gattgtcttc gtcaattttg
      961 tatcgtgcgg ctacttgctg tacaaatagc ttgtccagac cttcacggta ggaaaatatg
     1021 cgggagacat agtccagcgt tcttttcggt gtattgttgt tgcgaacctt tgttgttccg
     1081 gcgttgtaca tggccagggc agcgacctcg tttcccgaaa ggtcaaggca ataactgagg
     1141 tgagtcaatc cgttgtgtgc gtttgtctcg ggattaaaaa attctcgttc ggtgagcttt
     1201 ggaaaggcct tgttgtttaa ctgaaataaa ccccgatcta tggatgtagc gtttttgttt
     1261 accgcccgga tttggtagcg gctttcttcc caagccaggg caaaggcaag ggctggcggt
     1321 atattgttct tgctcgcatt atcaaggatt atcctgctga tttcactgtc gccagtgata
     1381 tgggtgtaaa accacaggac atcggcgcgg gctgcttcag acctatagag ggtaagggct
     1441 ggatcggttg ctctgccccc cttgattgct tgcaggtgaa attggtccgg ggcgatgatc
     1501 gaagccaggt gtacctcttc gtccgctcca aggatactca tggccattga atcaaaattg
     1561 gtataaatta tttttcccgc aagcacgacc agggcaaaaa taaatactgc aag
//
LOCUS       DAOQXQ010000470         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_94359_length_1610_cov_1.960129, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000470 DAOQXQ010000000
VERSION     DAOQXQ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94359_length_1610_cov_1.960129"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKH81_07650"
     CDS             complement(<1..631)
                     /locus_tag="PKH81_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ97949.1"
                     /translation="MPEQQNEYEDDIATLEAADESDTPVSAPRTLSPRAKQMIGLTDN
                     PTIPILIVDADLCIQHITQAARLLFSEYYQIEKKPFFNVFQQSLSLDEIRSLVRALHS
                     ADQGFGWSGNLRHKTRSTATLHTTANFVPFFNEDRSLAGHLVYFVDTTDVYKGNLRNT
                     FNGILEAAKLKDNDTGLHNERVSYYCRKMCEYLYDIHKYPQIDPDFIDSI"
     gene            complement(624..800)
                     /locus_tag="PKH81_07655"
     CDS             complement(624..800)
                     /locus_tag="PKH81_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97950.1"
                     /translation="MSLIPVPPPPTGISSIMISLAQEKELMENSAHSAYTKGIADHEG
                     PLYVRKSDAEIANA"
     gene            complement(877..1314)
                     /gene="tsaB"
                     /locus_tag="PKH81_07660"
     CDS             complement(877..1314)
                     /gene="tsaB"
                     /locus_tag="PKH81_07660"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNQ97951.1"
                     /translation="MNRIAIDTSGPILSVSVTGAATEAHISVKGEGQHAQSIIEVLEA
                     TLLKAEVPKTDIHRIYCALGPGSFTGLRIGYAAAKALALASDAPVIPISPLDCYARSY
                     SFWPGAVIAVIDARKERFYLQVFRSGKPVTDILDIQCSISFLS"
     gene            complement(1311..>1610)
                     /gene="tsaE"
                     /locus_tag="PKH81_07665"
     CDS             complement(1311..>1610)
                     /gene="tsaE"
                     /locus_tag="PKH81_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNQ97952.1"
                     /translation="VLGLDIEEPVTSPTFTLISEYEGRLHLYHMDAYRLDCAEAFIDL
                     GVEDYLGERGVCIIEWSERVMEELPSTTILISIEHTDGENRTITITNWPYGELSI"
BASE COUNT          403 a          356 c          420 g          431 t
ORIGIN      
        1 cgatagagtc gataaaatcg ggatcgattt gcgggtactt gtgaatgtca tagaggtact
       61 cacacatttt tcgacaatag tagctcactc gctcgttatg aagtccggtg tcgttatcct
      121 ttaattttgc cgcttccaga attccgttaa aggtatttcg gaggtttccc ttgtacacat
      181 cggtagtatc tacaaaataa accaggtgtc ctgccaggct tcgatcctcg ttaaaaaagg
      241 gtacaaaatt tgcggtggta tgcagtgtcg ccgtagagcg agttttgtgg cggagattcc
      301 ctgaccagcc aaagccctgg tcggccgagt gtagggctcg aaccagagat cgtatctcgt
      361 caagggaaag actctgctgg aatacgttaa agaagggttt tttttcaatt tgatagtatt
      421 cagaaaacag tagccgagct gcctgggtta tatgctgaat acacagatcg gcatctacaa
      481 tcaatatggg aatggtgggg ttatcggtaa ggccaatcat ttgtttggcg cgtggtgata
      541 acgttcgtgg tgcggaaacc ggagtatcag attcatccgc agcttctagg gtcgcaatat
      601 catcctcgta ttcattttgt tgttcaggca ttggcaattt ccgcgtcact ttttcggaca
      661 tacagcggcc cttcgtgatc cgctattcct ttagtatagg cggaatgagc cgagttttcc
      721 atcagttcct tctcttgggc aagggaaatc ataattgagg agatgcccgt aggaggcggg
      781 ggaacgggga taagggacat atcggctcta atggcatgga gagcttcgca aaaggtaagc
      841 gcatcatatc cggttaccag tattcgctct tccggatcaa gataggaaag agatgctgca
      901 ctgtatatcc aatatgtcgg taaccggttt tcctgagcgg aacacttgta ggtaaaaccg
      961 ctctttcctg gcgtcaataa cggcgataac cgcgcctggc caaaaggagt aggatcgagc
     1021 gtagcaatca aggggagata tcgggataac aggggcgtct gaggccaggg caagggcctt
     1081 ggccgcagca tatccgatac ggagcccggt aaaggatcca ggccccaggg cacaataaat
     1141 gcggtgtata tctgttttgg gaacttcggc tttgaggagg gttgcctcca gaacctcgat
     1201 tatgctttgg gcatgttgac cttcgccctt tacagaaata tgtgcttcgg ttgccgcacc
     1261 tgttacggat accgaaagga taggaccgct tgtgtcaatg gcaatacgat tcatattgac
     1321 agctctccat aaggccagtt tgtaatggtt attgtacggt tttcaccgtc ggtatgttca
     1381 atggatatga gtatcgttgt agaaggtaat tcttccataa cgcgttcgct ccattcgatg
     1441 atgcacaccc cgcgctcacc cagataatct tccacgccca aatcaataaa ggcctctgcg
     1501 caatcaagtc tatacgcgtc catgtggtat agatgcaagc gaccttcata ctcagaaata
     1561 agggtaaagg ttgggcttgt tactggctct tctatgtcca atcccagaac
//
LOCUS       DAOQXQ010000471         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_94497_length_1608_cov_3.483580, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000471 DAOQXQ010000000
VERSION     DAOQXQ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94497_length_1608_cov_3.483580"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /gene="rpsK"
                     /locus_tag="PKH81_07670"
     CDS             <1..353
                     /gene="rpsK"
                     /locus_tag="PKH81_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNQ97953.1"
                     /translation="KSVYEGNVYIQATFNNTIVTITDLNGNALSWASSGGLGFNGAKK
                     STPFAAQTVAETAVQKALTYGLREVHVFVKGPGVGRESAVRSLGALGLRVKSISDVTP
                     IPHNGCRPRKTRRI"
     gene            368..1003
                     /gene="rpsD"
                     /locus_tag="PKH81_07675"
     CDS             368..1003
                     /gene="rpsD"
                     /locus_tag="PKH81_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNQ97954.1"
                     /translation="MARYTGPLCRLCRTEQKKLFLKGDRCKTDKCPINKKRPIPGKDP
                     KARIGKRSDYGVQLREKQKLKRTYGMLEKQFRIFFDRALRMPGITGENLIGLLERRLD
                     NVVFRMHFAVNRNQARQIVQHGHILVNGKPVNIPSYLVKAGDVVEIKEVSKKLNVVKD
                     ALMEISKSGTYPWLSVDADALKGTFVSVPHRADVTDLADVKEQLVVELYSK"
     gene            1015..>1608
                     /locus_tag="PKH81_07680"
     CDS             1015..>1608
                     /locus_tag="PKH81_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ97955.1"
                     /translation="MARKNLLKGFKKPKGLTFEHDESTSDYGKFVASPFETGFGTTVG
                     NTLRRVLLSSIQGYAISAVKITSYDADGVPHNISSEFETIPNVVEDTLEVLNNLKLIR
                     LRLPEDVDQDTFLYEFTGPVNVTSDMFARDSQLEILSEPFHIFQMMEGGKVEFEVQVD
                     FGRGYVPAEVNERYIEVVGTIPLDAIYSPVTRVRYKIE"
BASE COUNT          426 a          402 c          400 g          380 t
ORIGIN      
        1 agaaaagtgt atacgaaggt aatgtataca tacaggcaac gttcaataac acgatcgtta
       61 caataaccga cctcaatgga aatgctcttt catgggcatc ttccggtggt cttggattta
      121 atggcgcaaa gaagtcaacg cccttcgcgg ctcagactgt tgcggaaact gcggttcaga
      181 aggcccttac ctatggtctt cgtgaagtgc atgtatttgt caagggtccc ggcgttggac
      241 gtgagtctgc cgttcggtct ctgggcgctc tcggtttgag ggtaaagtca attagcgacg
      301 taacccccat tccccacaat ggctgccgtc cccggaaaac ccggcgtatc tgacagagga
      361 gacatacatg gctagatata caggacccct ttgcagactt tgccgcacag agcagaaaaa
      421 actcttcctt aagggagatc gctgcaaaac tgacaagtgc cccatcaata agaaacgccc
      481 catccccggt aaggacccca aggcccgtat tggtaagagg agcgattatg gtgttcagct
      541 tcgcgaaaag cagaaactca agagaaccta cggtatgctc gaaaagcagt tccgtatctt
      601 ctttgaccgc gctcttcgca tgcccggtat caccggcgag aaccttatcg ggcttctcga
      661 gcgacgcctt gataacgttg tgttcagaat gcactttgcc gtaaaccgca atcaggctcg
      721 gcaaattgtt cagcacggac acattttggt aaacggcaaa cccgtgaaca tccccagcta
      781 tctcgttaaa gcgggtgacg ttgtggaaat caaggaagtt agcaagaagc ttaacgtagt
      841 caaggatgcc ctgatggaaa tttccaaatc aggaacctat ccctggcttt ccgtagacgc
      901 tgacgctctt aagggaacct tcgtatccgt acctcatcgt gcggacgtaa cagaccttgc
      961 agacgtcaaa gagcagctcg tcgtcgagct ctattctaaa taagaggagt tcgaatggcc
     1021 cgcaaaaatc tgttaaaagg atttaaaaag ccgaagggtc taaccttcga gcatgacgag
     1081 tctacatctg attacggaaa atttgtggct tcccccttcg aaaccggctt tggaactacg
     1141 gtgggtaaca ccttgcgccg cgtgcttttg tcttcaatcc aggggtacgc gattagcgcg
     1201 gtaaaaatta cctcttacga tgcagacgga gtaccgcaca acatctcaag cgagtttgaa
     1261 accattccta atgtggtgga agacactctt gaggtgttga acaaccttaa gctgatccgt
     1321 ttacggctgc ctgaggatgt tgaccaggat accttcctgt acgaatttac cgggccggta
     1381 aacgttacga gcgatatgtt cgcccgcgac agccagctcg aaatactgag cgaacccttc
     1441 catatctttc agatgatgga aggtggcaaa gttgaattcg aagttcaggt cgattttggt
     1501 cggggatatg tacctgccga agttaacgag cgctatatcg aagtagtggg aacaattccc
     1561 ctcgacgcga tttactcgcc cgtgacccgc gttcgctaca aaatcgag
//
LOCUS       DAOQXQ010000472         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_94642_length_1607_cov_2.248711, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000472 DAOQXQ010000000
VERSION     DAOQXQ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94642_length_1607_cov_2.248711"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PKH81_07685"
     CDS             <1..1232
                     /locus_tag="PKH81_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNQ97956.1"
                     /translation="REGKEYGDAARELFDRAIRTAQNSRDRDVAIWYLLDQSLAERTS
                     LCLDDIERYGPQWSDGSYYADILERLTMSIIRDRSWSSLERLSRILPDATDRDSRARV
                     DYLSARLAPIPAEEKTLALRRAWEGDHDSLYYRVLAADALGLPLGEEQLPVAGRGIPR
                     EDANAASRAASVLAVLESMVRYGLYDRVYPFARERAADIGLYATSMEVHRLADALSGA
                     GFYSEALRLSVLAFRSSSGSISNEDLAYIYPRPHQGFVATAAERFGIPEYLLYALLRS
                     ESFFNEKVVSHAGAMGLAQLMEPTARDIARKLKVETWDLLDPATNITFGAYYLSELIY
                     RLDGRVMSALFAYNAGISRVRTWEREGKDLPDDAFAESLPFTETREYGRKVLAAAAVY
                     GYLYYNVSPGQVVRDIF"
     gene            1257..>1607
                     /locus_tag="PKH81_07690"
     CDS             1257..>1607
                     /locus_tag="PKH81_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNQ97957.1"
                     /translation="MSIVQAIFLGALQGLAEFLPVSSSGHLALAAYFMELSEVPLLFD
                     ILLHLATLAAVVVVFRVRIIELCLTLGRLITRRSSTEDARNTSLILALIAGTMVTGVL
                     GFALKDLVENLSPFW"
BASE COUNT          305 a          403 c          454 g          445 t
ORIGIN      
        1 cccgcgaagg aaaagagtat ggagatgcgg cacgcgagtt gtttgaccgc gcgattcgca
       61 cggcacaaaa tagccgggat cgggatgtgg caatctggta tcttcttgat caatcccttg
      121 ccgaacgaac ctcgctgtgt cttgacgata ttgagcgcta tggcccgcaa tggtcggacg
      181 gttcctatta tgccgatata cttgagcgcc taacgatgtc cattataagg gatcgctcct
      241 ggtcgtcgtt ggaacgcctt tcccgcattc ttcctgatgc tacagacagg gatagccgtg
      301 ctcgtgtgga ctatctgagc gcgcgccttg ccccgatacc agcggaagaa aaaacgcttg
      361 cccttaggcg agcctgggaa ggggatcatg attctctgta ttatcgagtt cttgcggcag
      421 acgcccttgg cttaccgctt ggcgaggagc agttgccggt tgccggacgg ggtattcccc
      481 gcgaagatgc gaatgcggcc agcagggcgg cctcggtact tgccgttctt gagtctatgg
      541 tgcgctatgg gctttacgat cgggtgtatc cctttgcgcg cgagcgggca gctgatattg
      601 gcttatacgc aacgagtatg gaagttcatc gacttgccga cgctctttcc ggtgcgggtt
      661 tttatagcga ggctttgcgt ctttccgtat tagccttccg gtcatcctcc ggtagtattt
      721 ccaacgagga tcttgcgtat atatatccac gtccgcatca gggctttgtt gcgaccgcgg
      781 ccgaacgttt tggcatccct gagtatcttc tgtatgcgct attgcgaagc gagagttttt
      841 ttaacgagaa ggtggtctct catgccgggg cgatggggct tgctcaattg atggagccaa
      901 cggcacggga cattgcccgc aagcttaagg tagagacctg ggatcttctt gatccggcaa
      961 ccaatattac ctttggggct tactatttat ccgagttgat ataccggctt gacggcaggg
     1021 tgatgagcgc cctgtttgcc tataatgcgg ggatttcccg tgtacgcacc tgggagcgag
     1081 agggaaagga tttgccggac gatgcctttg ccgaaagttt gccgtttacg gaaacccgtg
     1141 agtatggccg aaaggtgctt gcggccgcag cggtttacgg gtatctttat tacaacgtgt
     1201 cccccggaca ggttgtccgc gatatatttt aatcctttac tttccggagc gtcctcatga
     1261 gcatagtaca ggctattttt cttggagcct tgcaaggcct tgccgaattt cttcctgtgt
     1321 ccagttccgg ccatcttgcc ctggccgcct attttatgga attgtccgaa gtaccccttc
     1381 tttttgatat ccttttacat ctggcaaccc tggctgcggt tgttgtagtg tttcgggtgc
     1441 gcattattga attatgcctg acccttggtc gcctgattac ccgccggagc agtacggaag
     1501 atgctcgcaa tacctccctg atactggccc ttattgcagg aacgatggta acaggggtgc
     1561 ttggctttgc gcttaaagat cttgtggaaa acctttctcc gttttgg
//
LOCUS       DAOQXQ010000473         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_95415_length_1599_cov_2.000000, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000473 DAOQXQ010000000
VERSION     DAOQXQ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95415_length_1599_cov_2.000000"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="PKH81_07695"
     CDS             complement(<1..114)
                     /locus_tag="PKH81_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNQ97958.1"
                     /translation="MHIIPIASGKGGVGKSLLSANLAIALGQAGKRVLLADM"
     gene            complement(105..461)
                     /locus_tag="PKH81_07700"
     CDS             complement(105..461)
                     /locus_tag="PKH81_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97959.1"
                     /translation="MYELVKLFFLMRDGTGSGLQSLSWYAGAPLLVIPPVLFFLFTMD
                     ETSFAPWLRLASLIKVLSVPSFVAFIVAAVPLAREFGLTGGGQELTNMALAGIFALVD
                     GAIALYCFGRGRILCT"
     gene            550..>1599
                     /locus_tag="PKH81_07705"
     CDS             550..>1599
                     /locus_tag="PKH81_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ97960.1"
                     /translation="MTKDQALSDNTFMNPKRLWSSALTFALLLLCFTLFSPALFARGT
                     ETDGSTERYLQIFQYVFGFVQENYVDEVDPRVLYEGAMRGMLDSLGDPYTSYIDSKSI
                     LGLNLKDTTTGAFGGVGLSITKPLLSTPEKPAYVEVASPIEDTPGWKAGIQPGDLILR
                     IGETDTAEITMEEVLALLRGPVDTEVTIRVRRGKLLEFPVTLKRALIEVPTVKFEMMD
                     KGIGYLRIIEFTPLTPGRVQDALESFEKAGYTSLLIDLRNNPGGLITSVVDVADKFID
                     SGVIVSTKSRISYENREFKAKSTTTTFKKDIPVVVLINKGSASAAEILAGALKDYHLA
                     YLVAEKVGFEGNIVFN"
BASE COUNT          417 a          389 c          387 g          406 t
ORIGIN      
        1 catatccgcg agcaagaccc gcttgcccgc ttgaccgagt gcaatagcca gattggcaga
       61 aagtagactc tttcccactc cgcccttacc gcttgcaatt ggaattatgt gcatagtatt
      121 cgacctcttc caaaacagta cagggcaatg gctccgtcaa ccagggcaaa aatcccggcc
      181 aaagccatgt ttgtcagttc ttgtccccct ccggtcaagc caaactcgcg ggcaagggga
      241 acggcagcta caataaaggc gacaaaagag ggtaccgaca gtaccttgat aaggctagcc
      301 aggcggagcc agggggcaaa cgaggtttca tccatggtga acaaaaaaaa cagaactggc
      361 gggataacaa gtaggggtgc tcccgcgtac cagcttaagg attgtaagcc agatcctgtt
      421 ccgtcccgca tcaggaaaaa cagtttaact agctcataca tgatgaatat cgggtacgtt
      481 tttggtctgg gtcgcattat atagaagtct acgcaacatg accgctccgg tcaaggggtt
      541 ttatcaagct tgacaaagga ccaagctctg tctgacaata cctttatgaa tccaaaacga
      601 ttatggtcca gtgccctgac ctttgccttg ttgctcttgt gttttaccct tttttccccg
      661 gcgctctttg ctcgtggaac agaaaccgac ggctcaactg aacgatatct tcagattttt
      721 cagtatgtgt ttggctttgt acaggaaaat tatgtagacg aggtagatcc tcgtgtttta
      781 tacgagggtg ccatgcgagg catgctcgat tctcttggtg atccctacac aagctatatc
      841 gactcaaaaa gcattcttgg gttaaacctc aaagatacaa caacaggggc ttttgggggc
      901 gtcggtttgt ctattaccaa gcccttgctc tcgactccag aaaaacctgc ctatgttgag
      961 gtggcttctc caatagagga tacacccggc tggaaggccg gtattcaacc aggagacttg
     1021 attcttcgca taggcgaaac tgataccgca gaaatcacca tggaagaggt ccttgccctg
     1081 cttcggggcc cggttgatac cgaggtaacc attcgcgttc gtcgggggaa gctcctggaa
     1141 ttcccggtaa cccttaagcg ggccctgatc gaagtaccta cagtaaaatt cgagatgatg
     1201 gataagggaa ttggttattt acgcattatc gaatttactc cgctaacccc tggccgggtt
     1261 caagatgccc tggaatcctt cgaaaaagcg ggctacacat cactgcttat tgatcttcgt
     1321 aataatcccg gtggtcttat cacgagtgtt gtggatgttg cggacaagtt tattgattca
     1381 ggtgttattg tatctaccaa atcgcgcatt tcctacgaga atcgggagtt taaggcaaag
     1441 agcaccacaa ctaccttcaa gaaggatatc cccgttgtgg tccttatcaa caagggttct
     1501 gcaagcgccg cggaaatcct tgccggagcc ctaaaggact atcatcttgc ttatctggtt
     1561 gcagaaaaag ttggcttcga aggaaacatt gttttcaat
//
LOCUS       DAOQXQ010000474         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_95465_length_1598_cov_3.147764, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000474 DAOQXQ010000000
VERSION     DAOQXQ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95465_length_1598_cov_3.147764"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1418
                     /locus_tag="PKH81_07710"
     CDS             228..1418
                     /locus_tag="PKH81_07710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNQ97961.1"
                     /translation="MDRIFLKKREDQRIRLGHPWIFDNEIGRYQGECPTGSPVQVYSA
                     DGLYLGTGIMNRNSRICVRIALRDSERVMDRAFFSERITNALSLRERLYAPEDSCRLV
                     FAESDLIPGLIIDRFVSIEGKIFLSIQFLTLGIDIFRDEIISILHELISPDGIFERSD
                     VHVRALEGLPERKGWIGSAHDPRIVIRENGVLLRVDVENGQKTGYFLDQKANRALVAA
                     LSRGMRVLDTFTHTGAFGLNAWKGGAISVESIDISREAIETVQEAIKLNGASPCVTAR
                     EADVFNVLKEYDTQERRFDIIVLDPPAFTKNARMVDKAYGGYKEINLRAMKILEPGGF
                     LITCSCSHFFTPEHFYHMLQNAAQDAKRTVQIIERTGAGPDHPMLSGYPESDYLTCAV
                     LRVW"
     gene            1412..>1598
                     /locus_tag="PKH81_07715"
     CDS             1412..>1598
                     /locus_tag="PKH81_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HNQ97962.1"
                     /translation="MVASSAGLSNALVVLGPTACGKTSLGVHLCTLFGGEVISADSRQ
                     VYRSLDIGTGKDLHEYGE"
BASE COUNT          391 a          376 c          404 g          427 t
ORIGIN      
        1 atgaaacgcc atcaattgtt tcaatccaat ccccgagttc tgcaggatct gccagatcct
       61 ccgtttgtat taaagctgcg ttagcctcgg gcggcgggag ttggacttct gcggttacaa
      121 ggccatgaca aagaatacag gttacgacga taatctttga tacattcata cccaagaata
      181 acacaccagt tggcttaagg caacgaaaca gctatactcc ctctactatg gacagaatct
      241 ttttaaagaa gcgggaagat cagcgaattc gtctgggaca tccctggatt tttgacaatg
      301 aaattgggcg atatcaaggg gaatgcccaa ccggctcgcc agttcaggtg tatagtgcgg
      361 acggcctgta cctgggcacc ggaatcatga accgaaactc acgcatctgt gtgcgcattg
      421 ccctgcgcga cagtgagcga gtaatggatc gcgctttttt tagcgagcgc atcactaatg
      481 cgctgtccct tcgcgagcga ctgtatgcac ctgaggattc ttgtcggctt gtattcgctg
      541 agtctgacct tatccctggc ctgatcatag atcgctttgt gtccattgag gggaagattt
      601 ttttatcgat acagtttcta acccttggca ttgatatctt tagggatgag attatcagta
      661 tcctgcatga attgatctcc cctgacggaa tctttgagag aagcgatgtc catgtacggg
      721 ccctggaagg tttacccgag cgtaagggat ggattggctc tgcccatgat ccgcgaattg
      781 tcatcaggga aaatggagtt cttcttcggg ttgatgtgga aaacggtcaa aaaaccggct
      841 attttctcga tcaaaaagct aaccgcgccc tggttgccgc tctgtcccgc ggcatgcgcg
      901 ttttggacac ctttacgcat accggtgcct ttggtttgaa tgcctggaag gggggagcga
      961 tcagtgttga atcaattgat atctcccggg aagcgataga aaccgtacaa gaagcaatca
     1021 agttaaatgg agcgtcgccc tgtgttaccg cacgggaagc tgacgtcttt aatgttctta
     1081 aagaatatga cacacaagag cgccgcttcg atattatcgt tttggatccc cctgctttta
     1141 caaaaaacgc acgaatggtg gacaaggcct atggaggcta caaggaaatt aatcttcggg
     1201 caatgaaaat tcttgagccg ggaggattct tgattacctg ttcctgttcc cattttttta
     1261 ccccagaaca cttttatcat atgttgcaaa acgcggctca agacgcaaag cgcaccgttc
     1321 aaattattga aagaactggc gcaggacccg atcatcccat gctctcgggg tatcctgaat
     1381 ctgattattt aacctgtgcg gtgctcaggg tatggtagcg tcttctgccg ggctatccaa
     1441 tgccctggtg gtccttggcc ctaccgcatg cggtaagact tcgctcggcg tacacctgtg
     1501 tacgcttttt ggtggagagg tcatttccgc agattcccga caagtatacc gttcccttga
     1561 tattggcacg ggtaaagatt tgcatgaata tggggaag
//
LOCUS       DAOQXQ010000475         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_95478_length_1598_cov_2.767336, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000475 DAOQXQ010000000
VERSION     DAOQXQ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95478_length_1598_cov_2.767336"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1393
                     /locus_tag="PKH81_07720"
     CDS             113..1393
                     /locus_tag="PKH81_07720"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ97963.1"
                     /translation="MAAKKTSKRDKVVLAYSGGLDTTVIIPWLKENYDYDVIAVCIDV
                     GQGDDWKTIKQRALDTGAAECYVVDAREEYITDFIYPALKANAVYEDQYLLGTSTARP
                     LIAKILVEYARQCKAVAISHGATGKGNDQIRFELTIKAFAPDLKIIAAWRDDKWNMDS
                     REAEIKFLEDRGIPVPMKKDQSYSRDENIWHLSHEGLELEETENEPNWKHMLQLTTVP
                     EEAPESGEYVTVEFDKGIPISVNGKKMKPLALMQELNKIGGRNGVGLIDIVENRVVGM
                     KSRGVYETPGGSILYTAHEMLDHLCLDRDTYFFKQQVSLKMSELIYNGRWYTTLMESL
                     MAFMDKTQETCSGWVKLKLYKGSIRPAGSHSPYSLYNANIASFTTGDLYNHKDADGFI
                     TLFGLPLKVRAMMEQSMGKGQAVDGKNLKKRRTE"
BASE COUNT          440 a          351 c          421 g          386 t
ORIGIN      
        1 ccgcccctgg taatcagtaa agaagacatt gataaaggcc tttcgattct aaaggccgta
       61 ttggcctcat aagtatctaa cgcgttgtag tggcgacata aggagtaagc agatggcagc
      121 aaaaaaaacg agcaagcggg acaaggttgt ccttgcctat tcaggcggac ttgacaccac
      181 ggtaatcatt ccctggctca aggaaaatta tgactatgat gttatcgcgg tgtgtattga
      241 tgttgggcag ggcgatgact ggaaaaccat caagcagcgt gcccttgata ccggtgctgc
      301 tgagtgttat gtcgtagatg cccgggaaga gtatataacg gattttatat accccgccct
      361 caaggcgaac gcagtgtacg aggatcagta tcttttgggc acctctaccg cacgtcccct
      421 tatcgccaag attcttgttg aatatgccag acagtgcaag gctgttgcca ttagccatgg
      481 tgcaaccgga aagggtaatg accagattcg ttttgaactg accatcaagg cctttgcacc
      541 cgatcttaag attattgccg cctggcgcga tgataaatgg aatatggata gtcgggaagc
      601 cgagattaaa tttcttgaag atcggggaat cccggttccc atgaagaaag atcaatcgta
      661 tagcagggac gaaaatatct ggcacttgtc ccacgaaggc cttgagcttg aagaaaccga
      721 gaacgagcca aactggaagc atatgcttca actgaccacc gttcctgaag aagcgcccga
      781 gtctggcgaa tacgtaacgg ttgaattcga caagggtatt ccgatatcgg tcaatggcaa
      841 aaagatgaaa cccctggccc tgatgcagga attaaacaag atcggtggcc gcaatggggt
      901 agggttaatc gacatcgtgg aaaaccgtgt tgtcggaatg aaaagccgtg gcgtatacga
      961 aactcccgga ggctctatcc tgtataccgc acacgagatg cttgaccatc tgtgtctgga
     1021 tcgggatacc tatttcttta agcagcaagt ttccctaaaa atgagcgagc tcatttataa
     1081 tggtagatgg tataccactt taatggagag cttgatggcg tttatggaca agacgcaaga
     1141 aacctgctcc ggttgggtaa aactcaagct gtacaagggt tctatccgcc cggcgggctc
     1201 tcattctcca tatagcctgt ataacgcgaa catcgccagt tttaccaccg gcgatttgta
     1261 taatcataag gatgctgacg ggtttattac cctctttgga cttccgctca aggttcgcgc
     1321 catgatggag caaagtatgg ggaagggtca ggccgtagac ggcaagaacc ttaaaaaaag
     1381 acgaactgag taaacgcttg ttgtaataca aaggaggtat ccatgggcga gtttatcgtt
     1441 tcgaatgcag agagcgctcc actggacatg ttgttaaagc ctttttcctc aataggtcat
     1501 gactggatgc tcattaccgc gggaagaagc gttgaacgcg gcgaatggaa taccatgacc
     1561 gcctcctggg gcagttttgg tgtgttttgg aatcgcag
//
LOCUS       DAOQXQ010000476         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_96229_length_1590_cov_2.502280, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000476 DAOQXQ010000000
VERSION     DAOQXQ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96229_length_1590_cov_2.502280"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1590)
                     /locus_tag="PKH81_07725"
     CDS             complement(<1..>1590)
                     /locus_tag="PKH81_07725"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNQ97964.1"
                     /translation="HSIPAIEEIQDIVESVLIEANEVRIAKAYILYRARHEAIRDTKS
                     LLLDIDETMDGYLSQSDWRVKENANVNFSLGGLILHNSGTITANYWLKNIYPEEVAEA
                     HRTAAFHIHDLSMFSGYCAGWSIRQLIAEGLGGVPDKITSKPACHLSTLIQQIVNFLG
                     IMQNEWAGAQAFSSFDTYLAPFVKKDGLTDKEVKQCLQSFIYGVNTPSRWGSQAPFTN
                     ITLDWNCPQDLADKPAVVGGKDMPFTYGDCQKEMDTINRIFIELMLEGDAAGRGFQYP
                     IPTYNITQDFEWSSHNAQLLFEMTARYGTPYFQNFLNSDLNPADVRSMCCRLQLDKRE
                     LRKRGGGLFGSDEFTGSIGVVTINMPQIAYLAKTDAQFYARLDYLMEQAKISLRLKRK
                     VIERLLDGGLFPYTRRYLTHLNNHFSTIGICGMNEACLNFLGVDITDPRGKAFAEKVL
                     LHMRQKLADFQVETGDLFNLEATPAESTSYRLARHDKKKYPDIITSGDADPFYTNSSQ
                     LPVGYTADIFDALDHQEALQTK"
BASE COUNT          367 a          354 c          442 g          427 t
ORIGIN      
        1 tttggtttgc agtgcctcct gatgatcaag cgcgtcaaag atgtcggcgg tgtagccaac
       61 gggaagttga ctcgagttgg tgtaaaaagg atctgcgtct ccggatgtta taatgtccgg
      121 gtacttcttt ttgtcgtggc gtgcaaggcg ataagaggtg ctttccgccg gggttgcttc
      181 caggttaaaa aggtctccgg tttccacctg gaaatctgcg agtttctgcc tcatgtgaag
      241 aagcactttt tcggcaaagg cctttcccct cgggtcagta atgtctacgc caaggaagtt
      301 aagacacgcc tcgttcattc cacaaatacc aatggtagag aagtgattgt taagatgtgt
      361 cagataacgc ctggtgtagg ggaacagtcc tccgtccaaa aggcgttcaa taacttttcg
      421 cttaaggcga aggcttatct ttgcctgttc catcagataa tcaagacgag catagaactg
      481 tgcatcggtt tttgccaaat aggcaatttg gggcatgttg atggtaacaa cgccgatcga
      541 tccggtaaac tcatccgatc caaacagccc gccgcctcgt tttctgagct ctcgtttgtc
      601 cagctgaagt cggcagcaca tggagcgcac gtcggccgga ttaaggtctg aattaaggaa
      661 gttttggaag tagggcgtgc cgtagcgggc ggtcatctcg aacaaaagct gtgcattgtg
      721 gctcgaccat tcaaaatcct gagttatgtt gtacgtcggg atggggtact ggaaaccgcg
      781 tccggcagca tcgccttcaa gcatcagctc aataaagata cggtttatcg tgtccatttc
      841 tttttgacag tcgccatagg taaagggcat gtcctttccg ccgaccacgg ccggtttatc
      901 ggcaaggtct tgggggcaat tccagtccag ggtaatgttg gtaaagggcg cctgacttcc
      961 ccagcgactt ggtgtgttaa cgccgtagat aaagctttgg aggcactgct ttacttcttt
     1021 gtcggtaagg ccgtcctttt ttacaaaggg agcaaggtag gtgtcgaaag aactaaaggc
     1081 ttgtgcaccc gcccattcgt tttgcataat ccccaaaaag ttaacaatct gttgaatcaa
     1141 ggtagaaagg tggcatgctg gttttgaggt tatcttgtcg ggtacaccac caaggccttc
     1201 ggctataagc tggcggatag accagccggc gcaataaccg gagaacatgg aaagatcgtg
     1261 tatatggaag gccgcggtac gatgcgcttc tgccacttct tcgggataga tattcttgag
     1321 ccagtagttg gcggtaatgg ttcccgagtt gtggaggata agaccgccaa gggagaagtt
     1381 aacgttcgcg ttttctttga cgcgccagtc agactgactg aggtagccgt ccatggtttc
     1441 gtcaatgtcc agaagaaggc tctttgtgtc tcggatagct tcgtggcgtg cgcggtagag
     1501 gatgtatgcc ttggcgattc gcacttcatt ggcttctata aggacgcttt ctacaatgtc
     1561 ttgaatttct tcgattgcgg gaatggaatg
//
LOCUS       DAOQXQ010000477         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_97566_length_1577_cov_3.049277, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000477 DAOQXQ010000000
VERSION     DAOQXQ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97566_length_1577_cov_3.049277"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="thiD"
                     /locus_tag="PKH81_07730"
     CDS             complement(<1..770)
                     /gene="thiD"
                     /locus_tag="PKH81_07730"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNQ97965.1"
                     /translation="MIKVATIAGSDASGGAGLEADLKTFQEYGVYGMAAVTLIATMDG
                     DRDWAHQVFPLSEDVLRAQLHTIFDGIGLDALKTGMLGSTYAVDMAADMIRTHAIDRY
                     VLDPVMVCKGAGEALHPELNERVASVLLPLSLVVTPNLFEAGQLAGMDTPATIDEMKE
                     AARRIHARGARNVFIKGGSRLEGQASALDIFFDGKDFNQVESALIDTRWTHGAGCTVA
                     AAITAGLGLGLEPYEAVSRAKKFITYSLRSGFALNRWVG"
     gene            903..1523
                     /locus_tag="PKH81_07735"
     CDS             903..1523
                     /locus_tag="PKH81_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNQ97966.1"
                     /translation="MTTSSGVYIRKLVVTGALGALAIFLGITRLGIFPWISGASLTIL
                     HIPAIIGAILEGPVVGAGIGAIFGAFSFFQASTSPASPIDFAFRNPLVSLLPRILFPI
                     LTWAIFHFGSKIKILPAVVVASVAGTIIHTILVLTMLVVTAGSGVLTGGADGVTLWAV
                     IWAIFIANGFPEAVAAGIFTTLVVAAKMGISSKRNTSRLTDELKKN"
BASE COUNT          379 a          440 c          360 g          398 t
ORIGIN      
        1 ccaacccagc gattaagggc aaagccactt ctaaggctat aggttataaa ctttttagca
       61 cggctcactg cttcgtaggg ctcaagaccc agaccaagcc ctgcggtaat agccgcagcc
      121 acggtacagc ctgctccatg agtccagcgg gtatcaataa gcgcactctc tacctgatta
      181 aaatccttac catcaaagaa gatatccagg gcactcgcct gcccttcaag acgcgatcca
      241 cccttaataa acacattacg ggctccacgg gcgtgaatcc gtcgggcggc ttctttcatt
      301 tcatctatcg tagcgggggt atccataccc gcaagctgac cggcttcaaa cagattaggg
      361 gttaccacaa gagaaagcgg cagtaatacg gaggcaactc gttcattcaa ttcggggtgt
      421 agcgcttcgc ccgctccctt gcacaccata acgggatcaa gtacataccg gtcaatcgca
      481 tgggtgcgaa tcatatccgc ggccatatcc accgcgtagg tactccccaa catgccggtc
      541 tttaaggcat ccagaccaat cccgtcaaaa atggtatgca gctgagcgcg caagacatcc
      601 tcggaaaggg gaaatacctg atgagcccaa tcccgatccc cgtccatagt ggcaataagg
      661 gtaactgcgg ccatgccgta taccccgtac tcctgaaatg ttttaagatc tgcttcaagt
      721 ccggcgccac cggatgcatc ggacccggca atcgttgcta ctttaatcat gtataaaagc
      781 tcctcttgcc cagaattata gcgaaaacca ccgggcggaa acaatagcaa acgtatttta
      841 tcttgacact taacagctgt tttgtcatca tacaatcacg ttactatgta aaggagcaca
      901 caatgacaac atcttccggt gtgtacattc gcaaactggt tgttaccggc gctctgggcg
      961 ccctggcaat atttcttggt ataacccgtc tgggtatttt cccctggatc tcgggcgcat
     1021 cgctcaccat tctccatatc ccggcaatta ttggcgctat cctggaaggc cccgttgttg
     1081 gcgcaggtat cggcgcaatt tttggagcat ttagcttctt tcaagcaagc acgtctcctg
     1141 cctctcccat agactttgcc tttcgcaatc ccctggtatc cctgttaccc cgcatccttt
     1201 tcccgattct gacctgggcg atatttcatt tcggctccaa gatcaagatc ctaccggcgg
     1261 ttgtagttgc ctcggtagcc ggaacgatta tccatacaat tttggtatta acgatgctcg
     1321 ttgttaccgc tggttccggg gtgcttaccg gcggggctga cggggttacc ttgtgggctg
     1381 ttatttgggc aatctttatc gccaacggat ttcccgaggc agttgctgcg ggtattttta
     1441 ccaccctggt ggttgccgca aaaatgggga tttcttccaa acggaacaca tcacgcctga
     1501 cagacgagct aaagaagaac taaattactg ctcgcaggat tgttggtatt ttcctccttt
     1561 tcccataaaa aagtaag
//
LOCUS       DAOQXQ010000478         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_97774_length_1575_cov_2.832895, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000478 DAOQXQ010000000
VERSION     DAOQXQ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97774_length_1575_cov_2.832895"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1575)
                     /locus_tag="PKH81_07740"
     CDS             complement(<1..>1575)
                     /locus_tag="PKH81_07740"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ97967.1"
                     /translation="FRQIGSRCPGHPEFGYTDGVETTTGPLGQGVSTAVGMAIAESML
                     AARFNTADASIVNHYTYALVGEGCLMEGVASEASSLAGHLKLGKLIVFYDENRICIDG
                     PTDMTLTEDIGARYAAYGWQVLSGDMYDYAGIEALVAKAKADGRPSLVMLKSVIGKGA
                     ASVAGSAKAHGAPIGAEGIAAAKKDLGLDPAKDFQIAPLALDFFAARRKELAAAEADW
                     NSLFDSWSKKNPELRSQWDAAFAPGGVDPALLAKAELPSFAQGDSQATRNSSGTALNA
                     FAKVLPNLVGGSADLQGPNVVGIKDAKAYSPANPSGRYLHFGVREFAMAAITNGIQVH
                     GGFRAFCATFLVFADYLRPALRLSALMKLPAVYVLTHDSIYLGEDGPTHQPVETLASL
                     RIIPNVRVLRPADAEETVEAWKMALERHDGPSCLALSRQNLAVFAKDDPDWKHTIKCG
                     AYIARKGGDKPDVTILATGSEVTLALEAASKVTGKTVRVVSVVSKELFEAQDAVIQKA
                     IVPEGSRVISCEAGVA"
BASE COUNT          404 a          432 c          419 g          320 t
ORIGIN      
        1 aagcaacccc tgcttcgcag gagataactc ttgaaccctc tggtacaatt gccttctgga
       61 taacggcatc ctgagcctcg aacaattcct ttgataccac agagacaacc cgaacggtct
      121 tgccagtaac cttggaagca gcctccaagg caagcgttac ctccgagccg gttgccagaa
      181 tcgtaacatc gggtttatcc ccacccttac gcgctatata cgcgccacac ttaatggtgt
      241 gtttccagtc gggatcgtcc ttggcaaaaa ccgcaaggtt ttgacgggag agggcaaggc
      301 acgaaggacc gtcatggcgc tcaagagcca ttttccaggc ttccacggtt tcttccgcgt
      361 cggcaggacg aagcacgcga acgttgggaa taatacgcag agaagcaagg gtttccaccg
      421 gctgatgagt tgggccgtct tctccaagat agatggaatc gtgggttaac acatacacag
      481 caggaagctt cataagggca gaaaggcgaa gggctggcct gaggtaatcg gcaaacacca
      541 ggaaggtggc acagaaggcg cggaatcctc cgtgaacctg tataccgttg gtaatcgctg
      601 ccatggcaaa ctcgcgcacg ccaaagtgaa gatatcgacc ggaaggatta gcggggctgt
      661 aagccttggc atcctttatg cccacgacgt ttggaccctg caagtcagca gaaccgccaa
      721 ccaggttggg cagtaccttg gcaaaggcat taagggctgt tccggatgaa ttgcgtgtcg
      781 cctggctgtc gccctgcgca aacgagggaa gctctgcctt ggctaacagg gcgggatcta
      841 caccgccagg ggcaaaggcc gcgtcccatt gagagcggag ttccggattt tttttggacc
      901 agctatcaaa caagctattc caatctgctt ctgcggccgc aagctccttg cgtctggcag
      961 caaaaaagtc cagggcaagg ggggcaattt gaaagtcctt ggcaggatca agaccaaggt
     1021 ctttttttgc tgctgcaatg ccttcggcac caataggagc gccatgggcc ttggccgaac
     1081 ccgcaacgga cgcggcaccc ttaccaatga ccgacttgag cataaccaaa gaaggccggc
     1141 catccgcctt ggccttggca accagggctt cgattccggc gtaatcatac atatcaccag
     1201 aaagcacctg ccaaccatag gcagcataac gagcgccaat gtcctcggta agcgtcatat
     1261 cagtaggacc gtctatacag atccggtttt cgtcgtaaaa cacgataagc ttaccaagtt
     1321 taagatgccc tgcaagactc gaagcctcgg aagcaacacc ttccatcaag caaccctcgc
     1381 caacaagggc ataggtataa tgattaacaa tgctcgcgtc tgcggtatta aagcgggcgg
     1441 caagcattga ttcggcgata gccataccaa ccgcggtaga tacaccctga ccaagaggtc
     1501 ctgtagtggt ttctactcca tcggtatatc caaattcggg atgaccgggg cagcgcgaac
     1561 ctatttgccg gaaat
//
LOCUS       DAOQXQ010000479         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_98398_length_1569_cov_3.437252, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000479 DAOQXQ010000000
VERSION     DAOQXQ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98398_length_1569_cov_3.437252"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKH81_07745"
     CDS             <1..689
                     /locus_tag="PKH81_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ97968.1"
                     /translation="FMDIEGENLIGVFSANEYLTRANLMKAYDTDRAATPLYQAKTVA
                     VVGGGNVAMDAARMAYRLGADKVYCIYRRTRAEMPARSEEIQHAEEEGVEFLYLQNPT
                     RIVGDETGRVRAIEVLDYELGESDASGRRSPVAKAGTEHEIAVDAVIVALGNESNPLM
                     AQTTEGLDVTKRGNIVVDEEQRTSLKRVWAGGDIVLGAATVILAMGEGRKAAANINRY
                     LAGDEEEIAD"
     gene            689..1087
                     /locus_tag="PKH81_07750"
     CDS             689..1087
                     /locus_tag="PKH81_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ97969.1"
                     /translation="MAGRLYEFSLRTERQGFTDITAQVAEAAHKSGVSDGLCTVFCPH
                     TTAAITINENADPDVIGDMLYGLDKAFPDRSEFRHAEGNSAAHLKSSTVGASETVILS
                     GGRLVLGTWQGIYFCEFDGPRTRRFFVSIC"
     gene            complement(1084..1539)
                     /locus_tag="PKH81_07755"
     CDS             complement(1084..1539)
                     /locus_tag="PKH81_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97970.1"
                     /translation="MMVGPPGQTLIHLARDIQFLYSREFDMIGVGPFIPHPYTPLKDS
                     PAGSVHLTLRVVALLRLTTRNAWLPATTALGSMDKDYRVEALKAGANVLMPNFSPVDA
                     KKKYEIYPGKRCVTEKAGACAGCTEGIAHAAGLETDWTRADSLKRRGEG"
BASE COUNT          390 a          360 c          420 g          399 t
ORIGIN      
        1 agtttatgga tatagaaggc gaaaacttga tcggtgtatt tagcgccaat gagtatctaa
       61 cccgagcaaa cttaatgaag gcctatgata ctgaccgtgc ggcaactcct ttgtatcagg
      121 caaaaacggt tgctgtagtc gggggcggta acgttgctat ggacgctgcg cgcatggcgt
      181 atcgccttgg agctgataag gtctattgta tttaccgccg aacccgcgca gagatgcccg
      241 ctcgttcaga agaaatacag catgccgagg aagagggtgt agaatttctg tatctgcaaa
      301 accccacccg gattgttggt gacgaaacag gacgcgttcg tgcgatagag gtgctggatt
      361 atgaacttgg agaaagtgat gcttccggaa ggcgaagtcc tgttgcaaaa gctggaactg
      421 agcatgagat agctgtagat gctgttattg tagcccttgg taatgaatca aatcctctta
      481 tggctcaaac caccgaaggt cttgatgtaa ccaagcgagg aaatattgtt gtagacgaag
      541 agcagcgcac aagcctaaag cgtgtttggg ctggtggaga tatcgttctg ggcgcagcta
      601 cggttattct tgccatgggt gaaggacgca aggcagccgc taatattaat cgctacctag
      661 ctggcgacga agaagagatc gcagattaat ggctggcagg ttgtatgagt tctccctgcg
      721 taccgagcga cagggattta cggatataac cgcacaggtt gcagaagcgg cgcacaaaag
      781 cggcgtttcc gatggcttgt gtacggtgtt ttgtcctcat acaaccgccg ctattaccat
      841 aaatgagaat gctgatccgg atgttatcgg tgatatgcta tatggactcg acaaggcatt
      901 tccggatcgt agtgagtttc gtcatgctga aggaaattca gctgctcact taaaatcaag
      961 tactgttggt gcaagcgaaa ccgttatcct ttccggaggt cggctggtat taggcacctg
     1021 gcaggggatt tatttttgtg agtttgacgg accccgaacc agacgattct ttgtaagcat
     1081 ttgctaaccc tctccccttc tctttaatga gtctgcccga gtccaatcag tttccagccc
     1141 ggccgcatgg gcaatacctt ctgtacatcc cgcgcacgcc cccgcttttt ccgtaacaca
     1201 ccgcttcccc ggataaatct cgtatttctt ttttgcatct accggagaaa agttcggcat
     1261 aagtacgttg gctcctgcct ttagtgcttc cacgcgataa tccttgtcca tactgcccag
     1321 ggctgttgtg gcaggaagcc atgcattacg cgtggtgagc cgtaaaagtg caacaaccct
     1381 aagggtaagg tgtacacttc ctgccgggga atctttaagc ggagtatacg gatgcgggat
     1441 aaaagggccg acgccgatca tgtcaaactc tcgtgaatac aagaattgaa tatcgcgcgc
     1501 aagatggata agtgtttgac cgggaggtcc caccatcata ccgcttccag tttggtatca
     1561 aggttctgt
//
LOCUS       DAOQXQ010000480         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_98727_length_1566_cov_2.730642, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000480 DAOQXQ010000000
VERSION     DAOQXQ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98727_length_1566_cov_2.730642"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKH81_07760"
     CDS             <1..804
                     /locus_tag="PKH81_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97971.1"
                     /translation="FPSVLVMTTITTVDPDLSNLLEPGASSPQQRLGILSSARERGFS
                     TGVMAMPLCPFLTDTPENTHALFTDCAKAGAQFIYPGSLTLRPGRQKEHCFKILTEKN
                     PSLVQRYTQLYGENRQSGMPRLEPIKPLMRQWNDALRTMNMPSLIPHSIFKKHLSLCD
                     TVFVLLCHMDSLYAQRGVATDALKRATKRYATWLSDQRTQLRRKRIRVSPSDPFPITR
                     TLDDELRYKATKAELMYALFDNERLAHLISSLILNDSVFDYPSLSQMPG"
     gene            complement(755..>1566)
                     /locus_tag="PKH81_07765"
     CDS             complement(755..>1566)
                     /locus_tag="PKH81_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNQ97972.1"
                     /translation="SKKQVQYSNEALLFIANSYAREAGVRNFEKNLDKIHRKYATEIV
                     FGDRTEKEKIAPDKPAVQKMLGKPIFREDDIKSADRPGTAVGLAWTSMGGDTLIIEAI
                     TNPGKEGFNLTGQMGDVMKESAAIAWSWVKHYVTEAGEVGSEWYEKQVVHLHIPEGAT
                     PKDGPSAGITMATALLSLIRGKAIKPRLAMTGELSLTGQVLPIGGLKEKTIAARRNGI
                     KEIIIPDANVRDLEEIPEHVKKGITFYPVTRMEDVVTLAFAKGKGNRKPSR"
BASE COUNT          385 a          425 c          322 g          434 t
ORIGIN      
        1 tttccttcgg tacttgttat gactacaatt accaccgttg atcctgatct ttcaaacctg
       61 cttgaaccag gggcctcgag tcctcaacaa cgattgggaa tacttagctc tgcacgagag
      121 cgaggttttt ccaccggggt tatggccatg cctctgtgtc cttttctaac cgacacacca
      181 gagaatacac acgctctttt taccgattgc gccaaagcgg gcgctcaatt tatctatccc
      241 ggatccctta ccctcagacc aggacggcaa aaagaacact gtttcaagat acttacagaa
      301 aaaaatccgt cgttagtaca gcgatataca cagctttatg gagaaaaccg gcaatcggga
      361 atgccccgtc tggaaccgat aaaacccttg atgcggcagt ggaatgatgc gttacgcacg
      421 atgaatatgc cttctcttat tccgcattcc atattcaaaa agcacctttc gctttgcgat
      481 acagtatttg tactgctttg ccacatggac agcctgtatg ctcagcgtgg tgttgcaaca
      541 gacgccctaa agcgggccac gaaacgctat gcaacatggc tcagtgacca acgtacacag
      601 ctcaggcgaa aacgtatccg cgtttctcca agcgatccat ttcccataac acgaactttg
      661 gacgacgagc taagatacaa ggctaccaag gcggaactaa tgtacgctct ctttgataat
      721 gagcgacttg ctcacctcat ttcgtctctt atccttaacg actcggtttt cgattaccct
      781 tccctttcgc aaatgccagg gtaacaacat cttccatcct ggttaccgga taaaaggtaa
      841 tacccttctt tacatgctcg ggaatctctt ccaaatcccg tacatttgca tcggggatga
      901 ttatctcttt aataccattt cgtctggcgg caattgtctt ttctttaagc ccgccaatgg
      961 gcaatacctg cccggtaagg gaaagctccc ctgtcatggc gagtcttggt ttaattgctt
     1021 ttccccgtat cagggacaag agtgcggtcg ccatagtaat acccgcggaa ggtccgtcct
     1081 tgggagttgc accctcgggg atatgtagat gcactacctg cttttcatac cattcagaac
     1141 cgacttctcc tgcctcggta acataatgtt ttacccaaga ccaggcaatc gcggccgatt
     1201 ccttcataac gtcgcccatt tgaccggtca gattaaaccc ttctttacca ggattcgtta
     1261 tcgcttcgat aattaaggtg tcccctccca tactggtcca ggcaagacca acggctgttc
     1321 cagggcggtc ggcgctctta atatcatctt ccctaaaaat cggtttacct aacatctttt
     1381 gtacagccgg tttatcaggc gcaatttttt ctttctctgt cctgtcacca aatactatct
     1441 ccgtggcata cttgcgatgg atcttatcaa ggttcttctc aaaattacgt acccctgcct
     1501 cgcgggcata ggagttcgcg atgaataaca gagcttcgtt ggaatactga acctgttttt
     1561 tggaaa
//
LOCUS       DAOQXQ010000481         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_99096_length_1562_cov_4.074320, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000481 DAOQXQ010000000
VERSION     DAOQXQ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99096_length_1562_cov_4.074320"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /gene="aspS"
                     /locus_tag="PKH81_07770"
     CDS             <1..373
                     /gene="aspS"
                     /locus_tag="PKH81_07770"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757427.1"
                     /note="catalyzes a two-step reaction, first charging an
                     aspartate molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; contains discriminating
                     and non-discriminating subtypes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ97973.1"
                     /translation="ESDPGSVKGDLYDLVLNGYELASGSIRIHDPELQKRIFKIVGFS
                     EEEGQKKFGFLTEAFKYGAPPHGGIAPGLDRLVMLMAGETSIKEVIAFPKNSFAQSPM
                     DECPSSVDQTQLDELHIKFTE"
     gene            417..1424
                     /locus_tag="PKH81_07775"
     CDS             417..1424
                     /locus_tag="PKH81_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNQ97974.1"
                     /translation="MIMLFALISFFSCAKPVPKQTFFVLGTTCTINLGQAGTKDVYAE
                     CFSYLSELELVLSANRSDSNVEAINREAGVKPVVAHPYTLEVLTTALDIARMTNGAFD
                     PTIGPLVKLWGIGTEDERRPSNEEIDEARALVSWKMVQVDTEAGTVYLPVKGMRLDLG
                     AIAKGYAADGLSRLLSTRGVHRGIIDLGGNILTLGDKSPGQAWKIGIRDPRITRGGPV
                     MGLPVISKTVVTSGIHERFFVENGNHWHHIIDPATGYPAQSKLLSVSIIGSTSMIADA
                     LSTSVFIMGQEKGLDLVQKFPDIEAILIDNTNKVYVSPGLTNSISILDTAFIKASDTT
                     Q"
     gene            complement(1414..>1562)
                     /locus_tag="PKH81_07780"
     CDS             complement(1414..>1562)
                     /locus_tag="PKH81_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNQ97975.1"
                     /translation="FSITLVLEQGREYQFRYLMDESRWENDWAADKYSPSQYGNCDNS
                     VVIV"
BASE COUNT          433 a          379 c          339 g          411 t
ORIGIN      
        1 tgaatcagat cctggaagcg taaagggaga cctttacgat cttgtcctta atgggtatga
       61 acttgcctct ggctcaattc gaatccacga tccggaactt caaaaacgaa tttttaagat
      121 cgttggattc agcgaggaag aaggccaaaa gaagttcgga ttcctaacag aagcattcaa
      181 gtacggcgcg cctccccacg gcggtattgc tcccggcctg gatcgtcttg taatgcttat
      241 ggctggtgaa acatccataa aggaagttat cgccttcccc aaaaacagtt ttgcccaaag
      301 cccaatggac gaatgtccgt cttctgttga ccaaacccaa cttgatgaac ttcacatcaa
      361 atttaccgaa tagtcggttc cccaaaacac aacactcaat tttaagggcg aacagcatga
      421 taatgctgtt cgccctcatt tccttttttt cgtgcgcaaa accagtcccc aaacaaacct
      481 tttttgttct tggaacaacc tgtactatta acctgggcca agctggaacc aaagatgtat
      541 atgcagagtg tttttcgtat ttaagtgaac ttgaacttgt tcttagcgcc aaccgttcag
      601 atagtaatgt agaagcaata aaccgggagg ctggagtcaa acccgttgtg gctcatccct
      661 ataccctgga agttcttaca acagcgcttg atatcgcccg tatgacaaat ggagcctttg
      721 atcccaccat cgggcctctc gtcaagctgt ggggtattgg aaccgaagac gaacgacgcc
      781 cctctaacga agaaattgac gaggctcgcg ctcttgtttc atggaagatg gttcaggtag
      841 atacagaggc aggcaccgtg tatttacccg taaagggcat gcgtcttgat cttggcgcca
      901 tcgccaaggg ttatgctgcc gacggtcttt ctcgcctttt atcaacccgg ggagtacatc
      961 gagggattat tgatcttggt ggaaacattt taacccttgg agataaaagt ccgggacaag
     1021 cctggaaaat cgggattcgc gatccccgga taactcgagg ggggcctgtt atgggcttac
     1081 ccgtgatctc aaaaacagtt gttacctcgg gcatccacga acgctttttt gtagaaaatg
     1141 gaaaccactg gcaccatatt atcgatcctg caacgggtta ccctgctcaa agcaagcttc
     1201 tttcagtctc tataatcggt tcaacatcaa tgatcgccga tgccttatca acttcggtat
     1261 ttattatggg ccaggaaaaa ggtctggatc ttgtgcagaa attcccggac atagaagcaa
     1321 ttcttatcga caatactaat aaggtgtatg ttagtcctgg tcttacaaac tcgatatcaa
     1381 tactggatac agcctttata aaagcttctg atactacaca ataacgacag agttatcaca
     1441 attaccatat tgcgaggggg aatacttgtc cgcagcccaa tcattttccc atcgagattc
     1501 gtccatcaag tacctgaact gatactcccg tccctgttct aacacaagcg ttatcgaaaa
     1561 ca
//
LOCUS       DAOQXQ010000482         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_99122_length_1562_cov_2.948242, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000482 DAOQXQ010000000
VERSION     DAOQXQ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99122_length_1562_cov_2.948242"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKH81_07785"
     CDS             complement(<1..687)
                     /locus_tag="PKH81_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97976.1"
                     /translation="MKRLAQLIQRLPLIASLTLLLCLPAWGDTQRPFTIVILHSYNLE
                     YDWTAEIHAGLFDRLKTLVRPRLVCTEFLDWKRFPSEALIQNLASHLADKYAGIPVDV
                     IIASDDKALEFAARYRQQIFSDAPIVFTGVYEEAVPALTAGQDRITGVYENQDVKTTI
                     RHALSIQREARAAYIISDMNESGQAVEQKLREALNTIAPDLPVHSLSDNPIGVIEKIV
                     SRFTTKDLLFI"
     gene            complement(699..>1562)
                     /locus_tag="PKH81_07790"
     CDS             complement(699..>1562)
                     /locus_tag="PKH81_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ97977.1"
                     /translation="RSVFGSDIKFCVGGGALLEIRQQEFFNAIGVPIYQGYGLTENAP
                     IICANSVKRHKFGTSGTIIPTLEVKIMKDDGSECAPGETGQIVTRGDSVMKGYYKNPE
                     ETAKTIRDGALWSGDLGYIDQDGFLVVTGREKALLIAADGEKYSPEAIEEAVINSSRY
                     VHQIMAYNEQCKFTSALITLNMDTLRADMGDERNPDVILDAIAKDVLSFRTKQEYKTI
                     PPQWQPASFALIAEPFSEEYGLINSTLKLVRHKVKAHYQSRIQEMYASGVADIHLQGN
                     RDALEKIMGAT"
BASE COUNT          353 a          365 c          418 g          426 t
ORIGIN      
        1 aataaaaagc aggtctttgg tggtaaagcg ggatacgatt ttttctatta cgccaatggg
       61 gttgtcgctt aaggagtgga caggtaagtc cggggcaatg gtgttcaagg cttcgcgaag
      121 tttttgttcc acggcctgcc ccgattcatt catatcgctg ataatatagg cggctcgtgc
      181 ctctcgctgt atggaaagcg catggcggat agtcgtcttt acgtcctggt tttcgtagac
      241 tccggtaatt ctgtcctgtc ccgcggtcag tgcgggtacg gcttcttcgt agacaccggt
      301 aaacacaata ggtgcatcgg agaagatttg ttgtcggtat cgcgcggcga actccagcgc
      361 cttgtcatcg ctggcgataa taacatctac cggaataccg gcatatttat ctgcaaggtg
      421 ggaggcaagg ttttgtataa gggcttccga ggggaagcgc ttccagtcta aaaattcggt
      481 acaaaccaag cgtggtctga ccagggtttt aagtcggtca aagagtcctg cgtggatttc
      541 tgcagtccaa tcgtattcaa ggttatacga atgaagaatg acaatggtaa aaggtctttg
      601 ggtgtcaccc caggcgggaa ggcacaagag cagggtaagg gaagcaataa ggggaagtcg
      661 ctgtatcaat tgtgccaaac gcttcattac accgtcctct atgttgcgcc catgattttt
      721 tccaaggcgt cgcgatttcc ttgcaggtgt atgtctgcta cgcctgatgc gtacatttct
      781 tggattcgtg attgatagtg agccttaacc ttgtggcgaa caagcttgag tgtagaattg
      841 atcaggccgt attcctcgct aaagggttca gcaataagtg caaaggaggc gggttgccac
      901 tgcgggggaa ttgtcttgta ttcttgtttt gtccggaagg aaagcacatc ctttgctatc
      961 gcgtcgagta ttacatccgg attgcgctca tcgcccatgt cggcgcgtag tgtatccatg
     1021 ttaagggtga taagggcaga ggtaaacttg cactgttcgt tatatgccat tatttgatgt
     1081 acataccggg acgaattgat aacggcctct tcgatggctt cgggactata cttttcccca
     1141 tccgctgcga taaggagtgc cttttcccga ccggttacca ccagaaaacc gtcctggtct
     1201 atatatccca agtctcctga ccacagcgcg ccgtcgcgga tagttttagc cgtttcctca
     1261 gggttcttgt aatagccctt catcaccgaa tccccgcgcg ttacgatctg gcctgtctct
     1321 ccgggcgcgc attcgcttcc gtcgtctttc attattttca cttcaagggt ggggataata
     1381 gttccagagg tgccgaactt gtggcgctta accgaattgg cgcagataat cggggcattc
     1441 tcggtaaggc catatccctg atagatggga acacctatgg cgttaaaaaa ctcttgttgg
     1501 cgaatttcca gcagggcgcc accgcctacg caaaatttga tgtccgagcc aaagaccgat
     1561 ct
//
LOCUS       DAOQXQ010000483         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_99568_length_1558_cov_3.105788, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000483 DAOQXQ010000000
VERSION     DAOQXQ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99568_length_1558_cov_3.105788"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1558)
                     /locus_tag="PKH81_07795"
     CDS             complement(<1..>1558)
                     /locus_tag="PKH81_07795"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ97978.1"
                     /translation="AEMAIAGEKNGVDLVVVETMSDLYEMKAAVLAVKEHTSLPVIAS
                     ATFQENNRMLNGADPATVVTVMEGLGVDAVGFNCGGDLKAAQILAHTFLAHASIPVFM
                     EPNAGIPVVENGTTVFKIPPEEFADTMEEAARAGVRILGGCCGTVKEHIAAMVKRCSP
                     LTPLALNPKQTTCISSWSDTICIGDDPVIVGERINPTGKKRFKEALLAGDQEYILSEA
                     QSQIDSGAHILDVNVGLPGIDEASVMDQTVSALQRTFRTPLQIDSSESAVLDRALRHY
                     NGKALVNSVNGKKSVMDSVFPLIKKYGGVVVALTLDEEGIPPTAEGRLDIAKKIIKRA
                     GDFGIDKKNIIVDTLTLTVSSQQKEALETVKALSLVRKELGVRTILGVSNISFGLPLR
                     DQVNSIFLTACLSAGLSACIINPLSQSMMGAYHTWRALAGYDENCLSYIDQFTGAQAV
                     SVESRPQTLSDTDKKKSEASASPSDDSDLFSIILCGLRDRSQKAAHLLLETKSPVEII
                     DGYIVPALDRV"
BASE COUNT          356 a          395 c          390 g          417 t
ORIGIN      
        1 cgacacggtc aagcgcaggg acgatgtatc cgtcgataat ctctaccggg ctttttgttt
       61 ccaatagcag gtgagcggcc ttttgcgatc tatccctgag tccacaaaga ataattgaaa
      121 ataaatcact gtcgtccgag ggagatgcgc ttgcctcgct cttttttttg tcggtgtcag
      181 aaagtgtctg ggggcggctc tccacgctta ctgcctgagc tccggtaaac tggtctatgt
      241 acgaaaggca attctcgtca tatccggcaa gcgccctcca ggtgtgatag gcgcccatca
      301 tggactgcga aagcgggtta ataatacacg ccgaaaggcc agcagacaga caggcggtaa
      361 ggaatattga gtttacttga tcccgtaagg gtagcccaaa cgaaatattg gataccccca
      421 gaatggttct tactcctagt tccttgcgca ccagggaaag cgccttgact gtttccaacg
      481 cttccttttg ctgagagctg acggtcaagg tcagggtatc gacgataatg tttttcttgt
      541 ctataccaaa atcgcctgcg cgcttaataa tcttttttgc tatatcaaga cggccttccg
      601 ccgtaggagg aattccttct tcgtccaggg ttagggcaac gacgacgcct ccatactttt
      661 tgataagcgg gaatacgctg tccataactg atttttttcc gttaaccgag ttaaccagag
      721 ccttcccgtt ataatggcga agagcgcgat ccagaacggc gctttcgctt gaatcaattt
      781 gcaggggtgt gcggaaggtt cgctgaaggg cgctaacggt ttggtccatc acgctcgcct
      841 cgtctattcc cggcaatcct acatttacgt caagaatgtg cgcgcctgaa tctatttgcg
      901 attgcgcttc ggagagtatg tattcttgat ctccggcgag caaggcttcc ttgaatcttt
      961 tttttccggt cgggtttatg cgctctccca cgattacagg atcatcccca atacagatgg
     1021 tatcgctcca ggaggatata caggtcgttt gcttgggatt aagggcgagc ggggtgagcg
     1081 gtgaacagcg cttgaccatt gcagcgatat gttctttaac cgtgccgcag caaccaccca
     1141 ggattcgcac gcctgctctt gccgcttctt ccatggtatc ggcaaattct tcgggcggaa
     1201 tcttgaatac tgttgttccg ttttctacta cgggaatacc cgcgtttggc tccataaaaa
     1261 cagggattga ggcatgcgca agaaatgtat gtgcaagaat ttgggcagcc ttaaggtcac
     1321 cgccgcagtt aaatccaacc gcgtccacac caagtccttc cattacggta accacggttg
     1381 caggatcggc gccgttaagc attcgattgt tttcctgaaa agtcgcggag gcgataacgg
     1441 gtagcgaagt gtgttcctta acggctaata cagcagcctt catttcatac agatcgctca
     1501 ttgtctcgac aacaacaagg tctacaccgt ttttttctcc ggcaatggcc atctcggc
//
LOCUS       DAOQXQ010000484         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_99817_length_1556_cov_2.062625, whole genome shotgun sequence.
ACCESSION   DAOQXQ010000484 DAOQXQ010000000
VERSION     DAOQXQ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99817_length_1556_cov_2.062625"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PKH81_07800"
     CDS             <1..1077
                     /locus_tag="PKH81_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ97979.1"
                     /translation="YHTIAMGQFSVLSTMPLSIVYQAVFAVARQNILLTAIVLLLSIL
                     AVWFFSKTVSRPVMALVAASQKIEKGAYELEIEATTQDELGLLTESFAAMGRGLAERE
                     RIKETFGKFVNREIAEQALTGRLELGGVRRTATIFFSDIRSFTAISEKLAPEAVVEFL
                     NEYMTRMVDCIEKTHGVVDKFIGDAIMGVWGAPMTQGSAAKDAYMAIQAMLMMRSALV
                     EFNKGRGSTDKPVIRIGCGLNTGPCLAGQIGSAQRMEYTVIGDAVNLASRIEALNKPF
                     GTDILISENTLKLVADYVVVEPMPPIKVKGKEAPLQIYALINLKGHSGPQTLAEVRSL
                     LGIVPPQKQVNPEEEEVKYEILKK"
     gene            1058..>1556
                     /locus_tag="PKH81_07805"
     CDS             1058..>1556
                     /locus_tag="PKH81_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNQ97980.1"
                     /translation="MKFSKSDLLFTSVIILLSTLFSVLFVRELNATIFRSGEKQLGTV
                     IFKKKTATRKGGQTLRWERLKNESPVFRGDTLRTASGSEAQVFFLDGTSLDIYENSML
                     RLDSSSDLPELEILEGSLSLGSVAIDSQADTSRSIARVRIGDSVVEVDPTATVSFLRT
                     GDQVTV"
BASE COUNT          420 a          399 c          382 g          355 t
ORIGIN      
        1 tatcatacca tagcaatggg acaattttcg gtgttatcaa ccatgcccct ttctatagtg
       61 tatcaggccg tatttgccgt cgcacggcaa aacatactcc taaccgcgat tgtactgtta
      121 ttgtccatcc ttgccgtgtg gttcttttcc aaaaccgtat cccgccccgt catggccctg
      181 gtagccgcct cgcaaaaaat tgaaaagggc gcctatgaac tggagataga ggcgaccacc
      241 caagatgaac ttggcctgct taccgaaagc tttgccgcga tgggacgagg ccttgccgag
      301 cgggagcgca tcaaagaaac ttttggcaaa ttcgtaaacc gcgagattgc cgaacaagcc
      361 ttaaccggac ggctagaact tggaggggtc agacgaacag ccaccatttt cttttcggat
      421 ataagatcct ttaccgccat cagcgaaaaa ctcgcaccgg aagcagtagt agaatttctt
      481 aatgaataca tgacacgcat ggttgactgc atagaaaaaa cccatggcgt cgtagacaaa
      541 tttattggag acgccatcat gggcgtgtgg ggcgcaccga tgacccaagg ctccgctgca
      601 aaggatgcgt acatggccat acaagccatg ctcatgatgc gctctgcatt ggtggaattt
      661 aacaagggtc gcggcagcac cgacaaaccg gtaatcagaa tcgggtgcgg ccttaatacc
      721 ggaccctgcc ttgccggaca aatcgggtcg gcgcagcgta tggaatatac cgttattggg
      781 gacgcggtaa accttgcaag ccgcatcgaa gcccttaaca aaccctttgg caccgacata
      841 cttatttcgg agaacaccct aaagctcgtt gccgattatg ttgttgtgga gccgatgccg
      901 ccgattaaag taaaagggaa agaagccccg cttcagatat acgccctgat aaatcttaaa
      961 ggccacagcg gcccgcaaac cctggcggaa gtaagatccc ttttaggaat agtgcccccg
     1021 caaaaacaag tgaaccccga ggaagaagaa gttaagtatg aaattctcaa aaagtgatct
     1081 tctttttacc tcggtcatta tactgctttc taccctcttt tctgtactct ttgtacggga
     1141 gctcaatgcg accatttttc gctcgggaga aaaacagtta ggaacggtaa tctttaaaaa
     1201 aaagacagca acccgaaagg gcggacaaac acttcgatgg gaacggctta aaaacgagtc
     1261 tccggtattt cgcggagaca cgctccgtac cgcttccgga tcggaagcac aggtgttttt
     1321 tttggacgga acgagtcttg atatctatga aaacagcatg ctcaggctcg actcatcgtc
     1381 ggaccttcct gaacttgaaa ttttggaagg ctcgctgtct cttggctcgg ttgcaatcga
     1441 cagccaggca gacacatccc gctcaatagc tcgcgtgcgc ataggcgatt ccgtggtaga
     1501 ggtagatccc accgcgacgg tatccttttt acgaaccgga gaccaggtaa ccgtag
//
LOCUS       DAOQXQ010000485         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_100056_length_1554_cov_1.939960, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000485 DAOQXQ010000000
VERSION     DAOQXQ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100056_length_1554_cov_1.939960"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="flgB"
                     /locus_tag="PKH81_07810"
     CDS             <1..376
                     /gene="flgB"
                     /locus_tag="PKH81_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNQ97981.1"
                     /translation="AMDVNALRYGVAANNLSNSGVPNFKRTQVNFESELERALESEQR
                     AKGQFQLAVSNDRHIASDGIIDYQTIEPRRVLDYTSTVKANGNNVDPEQEAMDILKIQ
                     MNYQLLTQVQSFQFSQVNSVLR"
     gene            392..847
                     /gene="flgC"
                     /locus_tag="PKH81_07815"
     CDS             392..847
                     /gene="flgC"
                     /locus_tag="PKH81_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNQ97982.1"
                     /translation="MGLFTSINIAASGMTAERLRTDVISDNIANASTTRTQEGGAFRR
                     SRVVLAQKEGGIDWRNPFLPAQLDRGPGSGVRVMSVEKDTADLRMVYDPTHPDAIQSG
                     PKAGYVEYPNVNIVTEMVDLISASRSYEANSSVIQGSKEMFMRALEIGR"
     gene            853..1161
                     /gene="fliE"
                     /locus_tag="PKH81_07820"
     CDS             853..1161
                     /gene="fliE"
                     /locus_tag="PKH81_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNQ97983.1"
                     /translation="MNIDPVSVESFRSTMVSGKNLVDKANRPLTASFEQTLLAAFDKV
                     NEKQQVTTDIAQQMIVDPESVDVHDVTIAMAEANLSLKMAQSVIDRLIKGWNEITTNR
                     "
     gene            1243..>1554
                     /locus_tag="PKH81_07825"
     CDS             1243..>1554
                     /locus_tag="PKH81_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HNQ97984.1"
                     /translation="MNEWLKKLFEQISTLWKKWTVTQKAVLAGVILAAIAGLFILFSV
                     SAGPSMVPLLDTPITDESVRDKIILRLNEENIKTSVSATGIISVADESTARRMRSILI
                     RE"
BASE COUNT          451 a          316 c          375 g          412 t
ORIGIN      
        1 ggctatggat gttaatgccc ttcggtatgg ggttgccgcg aacaatcttt ccaattcggg
       61 tgttcccaat tttaaacgaa cccaggttaa ctttgagtca gaactggagc gggccttaga
      121 gtcggaacag agagctaagg gacagtttca gcttgcagtg tccaatgatc gacatattgc
      181 ctctgatggg attattgatt accagacaat agagcctcgc agggttcttg attatacctc
      241 taccgtaaaa gccaatggaa acaatgtcga tccggaacag gaagccatgg atatccttaa
      301 gattcaaatg aattatcaat tattgactca ggttcagagc tttcagttct cccaggtcaa
      361 ttcagtatta cgatagcagg agcgatagat aatgggtctt tttaccagca tcaatatagc
      421 agcaagcggt atgacagccg aacggctaag aaccgatgtt atctctgata atatcgcaaa
      481 tgcatctacc acccgcactc aggaaggcgg ggcttttaga agaagtcgcg tcgttttggc
      541 acaaaaagaa ggcggaatag actggagaaa tccttttttg ccagcacaat tggatagggg
      601 acctggctct ggtgttcgag ttatgtcagt agagaaagat acagcggacc tgcgcatggt
      661 gtatgatccc actcatcctg atgccataca aagtggtcca aaggccgggt atgttgagta
      721 tcccaatgtt aatatagtga cggaaatggt tgatcttatt tcggcttcgc gctcatatga
      781 ggcgaattca tctgtaattc agggttcaaa agagatgttt atgagagccc ttgagattgg
      841 taggtagaac gtatgaacat agatccggtt tccgttgaat catttcgcag tactatggtt
      901 tctggcaaga acttggtaga caaggcaaat cgaccgctaa ctgcgagctt tgagcaaact
      961 cttttagcgg cctttgacaa agttaacgag aaacagcagg ttacaacgga tattgcccaa
     1021 caaatgatag tagatccaga atcggtcgat gtacatgatg taaccattgc gatggccgaa
     1081 gcaaatttat ccctgaagat ggctcaaagt gtaattgatc gccttattaa gggctggaac
     1141 gaaattacga cgaaccgcta aaaacgtctg tcattctgtg ctatagtatg gtacaatata
     1201 caattaaaac ttgacaggtt cgttctgtaa cgggagggga gcatgaacga atggctgaaa
     1261 aagctatttg agcagatatc gacactatgg aaaaaatgga cggttaccca aaaagctgtc
     1321 ctcgctggtg tcatccttgc cgctattgcc ggcttgttta ttcttttttc agtctcggca
     1381 gggccatcaa tggttccttt gctggacacg ccaataaccg atgagtctgt tcgggataag
     1441 ataatacttc gtcttaacga agagaatatc aagacctctg tatcagcaac cggaataatc
     1501 tctgtagcag atgaatcaac tgctcgaaga atgcgttcaa tccttatccg cgag
//
LOCUS       DAOQXQ010000486         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_100378_length_1550_cov_3.968562, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000486 DAOQXQ010000000
VERSION     DAOQXQ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100378_length_1550_cov_3.968562"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..318
                     /locus_tag="PKH81_07830"
     CDS             184..318
                     /locus_tag="PKH81_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97985.1"
                     /translation="MAFKLDGAKFDTLDELVEALYPLYSDKMSIEEFKKYAEENAQQS
                     "
     gene            complement(395..>1550)
                     /locus_tag="PKH81_07835"
     CDS             complement(395..>1550)
                     /locus_tag="PKH81_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97986.1"
                     /translation="KKLADDNRILLQKTIAEALGLGTGENLFCLMQEQRSGLWYLRTS
                     NEIHANGLWASLNGFETQVFLNIREVEDEADGRYRTLYRSLNGKGVRDIHMALQAIVY
                     ADLYAALDEVLTPLFYSYFKTVCLPDSALSQNSESGPELPTNLKQAVHALYHVASTRL
                     NTTANDAGVNKATLTCMKFLSRLDKLCRRSSRTGQGPLVTIKGFPEALAASSIILALS
                     DILPKVNQSIALPDLIGQWSLDRRIADRYEEINFVSYLEVSEFRTHISLWAALEPLVR
                     IGANKGCIQAIITFISRLCAPQRAARNVVKQAITTLAGDTRVHREIRLHTWDGVAWFD
                     KEKAETLVHKTCTASLVFGPVKKVDRFERVAIAYLTNSEYRFEKLTQNVR"
BASE COUNT          404 a          357 c          364 g          425 t
ORIGIN      
        1 ttcctgattc acttaccggt attcgggaca aggttgccgg agcacagagt attccggtaa
       61 gcactgggca tctacgaaca attcttgaaa acttttctgt aaggggatga cgttccaggg
      121 tgtatttctt gcgctcttca acaaaatcca ttatactaaa gacgcatttt ccagggggtt
      181 cgcatggctt ttaaactaga tggagcgaag ttcgatacac ttgatgaatt agtggaggca
      241 ttgtatcccc tctattccga caagatgtct atcgaggaat tcaaaaagta cgccgaagaa
      301 aacgcccagc agagctgacg ttatcggtca cacatacaaa aacgcgcagg agtagatcct
      361 gcgcgttttt tttatccaac gatctgttac ttagttaacg aacgttctgt gtcagttttt
      421 caaaccggta ttctgaattg gtaagatatg caatagccac ccgttcaaat cgatcaactt
      481 tcttgacagg tccaaacacc aaagaagccg tacacgtttt gtgtaccaag gtctccgctt
      541 tttccttgtc gaaccaagcg acaccatccc aggtatgaag ccgtatttct ctgtgcactc
      601 gtgtgtcgcc agccaatgta gtaatcgctt gttttacaac attgcgggct gctcgctgtg
      661 gtgcgcatag ccgcgaaata aaggtgatga tggcttgtat gcatcctttg ttagcgccga
      721 ttcgtacaag aggttcaaga gcggcccaca aggaaatatg tgttcggaac tccgaaacct
      781 ccagatacga aacaaaatta atctcttcgt atctgtctgc tatgcgccga tcaagactcc
      841 attggccaat aagatccggt aaggcaatcg actggtttac cttgggtaat atgtcggaca
      901 gtgcaaggat gatagagctt gcagccaggg cttccggaaa accctttatt gttacaagag
      961 gtccttgtcc ggtacgcgaa gatcggcggc aaagcttgtc cagtcgagac aggaatttca
     1021 tacaggtaag cgttgcctta ttgactcctg catcattggc cgttgtgttg agtctggttg
     1081 atgctacgtg atataaagcg tgaactgcct gtttcagatt tgttgggagc tcgggtccac
     1141 tttcagaatt ctgcgacaag gctgaatctg gcaggcacac ggtcttgaaa tacgaataaa
     1201 aaagcggtgt taacacctca tccagggccg cgtacaaatc ggcgtacaca atagcttgca
     1261 aggccatgtg aatatcgcgt acgccctttc cgttaagaga ccggtacagg gttctataac
     1321 gtccatctgc ttcatcctca acctcgcgaa tattcaagaa tacctgagtt tcaaatccat
     1381 tgagagatgc ccacagacca ttggcgtgta tttcgttgga ggttcgcaga taccacaagc
     1441 cgcttcgttg ttcttgcatt aagcaaaaaa ggttttctcc tgttccaagc cccagggctt
     1501 cggcgatggt tttttgaagg agaatacgat tatcgtcagc taattttttg
//
LOCUS       DAOQXQ010000487         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_100398_length_1550_cov_3.019398, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000487 DAOQXQ010000000
VERSION     DAOQXQ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100398_length_1550_cov_3.019398"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKH81_07840"
     CDS             <1..464
                     /locus_tag="PKH81_07840"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNQ97987.1"
                     /translation="RGLLKEDTAGKTPWITEKRKVNVETDNPFDPAKTLPNALGYLLD
                     DHPNNRPALEYALSYLLLDKDLTAFMQYMQSAQSLYTKLPIHYQEAICIYYATVESNP
                     EAFSSYGIDRTVYERFIKFCSVAGNGPKALQQEFGNTYYYYAQFEETPQRN"
     gene            476..>1550
                     /locus_tag="PKH81_07845"
     CDS             476..>1550
                     /locus_tag="PKH81_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97988.1"
                     /translation="MKTRHVILAGSVVVVLSAGLLIGSSLQKSYPENAVTESRYPDLY
                     PDYTGVSVPSNMAPLNFKLDAVYDKSYVKVTNRDQSRQMECFGSHKVQFDMADWHNLL
                     QACAGDSLWIEVSARSKQQWTTWKKFAIYVSKDTIDPYMVYRLIEPGYEKWHIVGIYQ
                     RNLETFHEKPVIENKLTGYNCVNCHSFCNGNPDRMMFHMRADNGGTYIVSDGEIEKLK
                     TKTDQTVSNLTYPYWHPSGRYIASSVNTIRQFFHAVPGKKMEVFDAESDVVVYDVSQK
                     EILSKSSLITKDAYETFPSFSADGKWLYFCSAPALKMPEKYNQLRYSLCRVPFDAANG
                     TIGSQVDTLVHADSISVSFPRMSP"
BASE COUNT          405 a          386 c          382 g          377 t
ORIGIN      
        1 tgcgggggtt gctaaaggag gataccgccg gaaagactcc ctggattacc gaaaagcgga
       61 aggtgaatgt ggagaccgac aatccgttcg atcctgctaa aacgttaccc aatgccctgg
      121 gctatctgtt agacgatcat cccaacaacc ggccggcgct ggaatatgcc ctgtcgtacc
      181 tgcttttaga caaggatctg accgctttta tgcaatatat gcagtcggcc caaagtctgt
      241 atacaaaact tcccatccat tatcaggagg cgatctgtat ttattatgct acggtggagt
      301 ctaatcctga agctttcagc tcgtatggaa ttgaccggac cgtatacgaa cggtttatca
      361 agttctgcag tgtggcaggc aacggtccca aagcgcttca gcaggaattc gggaatacat
      421 attactatta cgctcagttt gaagaaactc ctcaaaggaa ctaaggatga aaagaatgaa
      481 aacacgccac gttatacttg ccggatcggt cgttgttgtg ctgtcggctg gactgttgat
      541 tggtagctcg cttcaaaaaa gctatcctga aaatgccgta acagagagcc gttatccgga
      601 tctgtacccc gattataccg gagtatcggt accctccaat atggctccgc ttaatttcaa
      661 gttagatgcc gtatacgaca aaagttatgt aaaggttacc aacagggatc aaagcaggca
      721 gatggaatgt tttggctctc acaaggtgca gtttgacatg gctgactggc acaacctgtt
      781 gcaggcatgt gccggcgact cgctgtggat tgaagtctcc gcccgttcca aacagcagtg
      841 gaccacctgg aagaagtttg ccatctatgt cagcaaggat acaatagacc cctatatggt
      901 ttaccgcctc atcgagccgg gatacgagaa atggcacatt gttgggattt atcagcgtaa
      961 tctggagaca ttccacgaaa agcctgtcat cgagaataag ctaacgggct acaactgcgt
     1021 gaactgtcac tcgttctgca acgggaatcc cgaccggatg atgtttcata tgcgtgccga
     1081 caatgggggt acttacatcg tatccgatgg tgagattgag aagctgaaga ccaaaacaga
     1141 tcagactgta agtaacctca cctaccccta ttggcaccct tcgggcagat acatcgcgtc
     1201 gtctgtaaac acgatccggc aattctttca tgctgtaccg ggtaaaaaga tggaggtatt
     1261 cgatgccgaa tcggatgtgg tggtgtatga tgtctcccaa aaagagattc tatcgaagtc
     1321 gtcgcttatt accaaggatg cctacgagac cttcccctca ttctcggccg acggcaagtg
     1381 gctctatttc tgttcggccc ctgccctgaa gatgcccgaa aagtacaacc agcttcggta
     1441 cagtctgtgc agggtaccct tcgatgctgc caacggaacc atcggttcgc aggtagacac
     1501 cctggtacat gccgactcca tcagtgtatc cttcccccgc atgtctcccg
//
LOCUS       DAOQXQ010000488         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_100524_length_1549_cov_2.945114, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000488 DAOQXQ010000000
VERSION     DAOQXQ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100524_length_1549_cov_2.945114"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..961
                     /locus_tag="PKH81_07850"
     CDS             134..961
                     /locus_tag="PKH81_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNQ97989.1"
                     /translation="MCDRDSISDYLREIRKYPLLTFEQEIDLSKRIQDGDLSARKKLI
                     ESNLRLVVTVARKYAAPDLPLLDIIQEGNLGLITAAQKYEFSYNVRFSTYACWWIQQS
                     ITRAIANKRRLIRLPFRKEELAGRIRRTAAELYQQLSRCPTHEEIAEALCINPREVMD
                     IMLSSSSVTSLDMQLDEDDSASLKDILPDSTYNPEDQFMKQYTHDCMMNILSELKDNE
                     RDIMMKRYNLVNDGNSYTLKTIGNQYGVSAETIRQIELRALRKLKSRSSELRDIVYS"
     gene            998..>1549
                     /locus_tag="PKH81_07855"
     CDS             998..>1549
                     /locus_tag="PKH81_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97990.1"
                     /translation="MERKKTHSPKKRAVRSKARSKKAGLFLRELKTNLVLAAVIFGFC
                     LALLLGRFVLWPVGESGVVSLPPVAEQSPTVQAPVPQAPAPAPPAPPPAVTSVPKTPS
                     AAPVKRPLATTPEKPMAERSRPAPAALRSPPEQPVPPRTKGSLILVFDDAGHNLDQLQ
                     KFLDLPFPCTIAVLPGLQYSKEAA"
BASE COUNT          394 a          388 c          353 g          414 t
ORIGIN      
        1 aatagtatac atttacctgt atgaaatatt ccctttaata tctctaatcc ctttccctgt
       61 aaggacttac atccgaccaa tcagtcgtct ttaaaagttg gcacggtaat tgcttaatgt
      121 aggcggggga tatatgtgtg atcgtgattc aataagtgac tatctcagag aaattagaaa
      181 gtacccgttg ctaacctttg aacaggaaat tgatttatcc aagcggatac aggatgggga
      241 tctttctgcc cgaaaaaaac tcatagagtc caacctgcgg cttgtggtta ccgttgctcg
      301 caaatacgca gcgcctgatc tacctttgct tgatatcatc caggaaggaa accttgggct
      361 tattactgcg gctcaaaaat atgagttttc gtataatgtc cggttttcca cctatgcctg
      421 ttggtggatt caacaatcaa ttaccagggc tattgccaac aagcgccggc tcattcgtct
      481 tcctttcagg aaagaagagc ttgccggcag aatcagaaga actgctgccg aattgtatca
      541 gcagctttcc cggtgcccca cgcacgagga aatcgccgaa gcattgtgca ttaatcctcg
      601 cgaggtaatg gatatcatgc tcagttcaag ttccgttacg agtctggata tgcagcttga
      661 cgaggatgat tcagcatcac ttaaggatat ccttcctgat tctacctaca atcccgagga
      721 tcagttcatg aagcaatata cccatgattg catgatgaat attctctcgg agcttaagga
      781 caatgagcgg gatatcatga tgaagcgcta taatctggtc aatgatggaa actcctatac
      841 tcttaagacg ataggaaatc agtacggggt ttccgcagaa acgatccgtc aaattgaact
      901 tagggccctg cgcaaactca aatcccgctc cagtgaactt agggacattg tgtatagttg
      961 acacgtcagg tagcagtctt tatccttccg tattgatatg gagaggaaaa agactcactc
     1021 acccaaaaaa agagccgttc gttccaaagc ccgctccaag aaagcgggct tgtttttacg
     1081 cgaactcaaa acaaacctcg tattagccgc agtaatcttt ggcttttgcc tggccctgtt
     1141 gctgggccgt tttgtgttat ggccagttgg ggaatcgggt gtggtgtctc tccctccggt
     1201 agccgagcaa agcccaacgg tacaagcgcc tgttccgcaa gctcctgctc ctgcccctcc
     1261 tgcgcctcct cctgcggtca cttcggtgcc aaagacgcct tcagctgcac cggtaaagcg
     1321 gcctttggct actacgcctg aaaagcccat ggcagagcgg agtcgacctg ctcctgctgc
     1381 actacgcagt cccccggaac agcctgttcc cccccgaaca aagggatctc tgatccttgt
     1441 ttttgatgat gcggggcata acctggatca attgcagaag tttcttgatc ttccctttcc
     1501 ctgtactatt gccgttttac cgggtttaca gtattctaaa gaagcagcg
//
LOCUS       DAOQXQ010000489         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_100764_length_1547_cov_2.221180, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000489 DAOQXQ010000000
VERSION     DAOQXQ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100764_length_1547_cov_2.221180"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKH81_07860"
     CDS             complement(<1..407)
                     /locus_tag="PKH81_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNQ97991.1"
                     /translation="MKVYIMTDLEGVAGVLDSKNWCFSEGRYYLKAKELLTMEVNAAI
                     EGFIAGGATEILVADGHGPGAVNQYQLHPRAEYSRNWASGLSAFGLADRKFDYAAWIG
                     QHPMAGTIGGHLCHTGNMGVVEQTVNGIKVGEFG"
     gene            complement(557..>1547)
                     /locus_tag="PKH81_07865"
     CDS             complement(557..>1547)
                     /locus_tag="PKH81_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNQ97992.1"
                     /translation="NTGIAPDKPGMNPAQAIYSKVLKKPINWTRYKHHTENGVEQMKV
                     YIMTDLEGVAGIMDYGNWCSEKSRYYENAKELLSKEINAAVEGFLEGGATEILIADGH
                     GSGAVNPELLHEKVELSRYGVSLFIAKEKIDFLAFVGQHPMAGTIGGHLSHSGNFSVV
                     EQTINGLIVGEFGDLVLTAGQQDIRTIFATGCEAFCGEAQALVPGIETVAVKRGTQTT
                     AGNHLPKEVYREHNKSAIHLHPAEARKRIKAGARKAIERAQKEKFGLIELPKPPYERI
                     RIMRGDSDYPPRILKQSHPKSILDMRHSKVSLYELEINPADPKLSKLIGKQIA"
BASE COUNT          314 a          419 c          342 g          472 t
ORIGIN      
        1 ccgaactctc caaccttgat gccgttgacc gtttgttcta cgacccccat gttgcccgta
       61 tggcacagat gtcccccgat agtccctgcc atgggatgct gacctatcca tgccgcgtaa
      121 tcaaacttcc tgtccgcaag gccgaaagcg gatagcccgg aagcccagtt ccgtgaatat
      181 tccgcccgtg gatgaagctg gtactggttg acagcgccgg gcccgtgtcc gtctgcaaca
      241 aggatttccg tagcccctcc tgcgatgaac ccttctatgg cggcgttgac ctccatggta
      301 agaagctcct tcgccttcaa gtaatacctt ccttcagaaa aacaccagtt tttggaatcg
      361 agaactcccg caactccttc caaatcagtc attatatata ctttcatctg ttccacctcc
      421 attttattgg aaatttttaa taccgtgcga taaatcgctt gtgccgggtt catcccgggc
      481 ttgtccgggg ctattccggt atttgtcccg taaggggcac cgctgcaatt tcctgtagat
      541 taacgactac aggatatcat gctatctgtt ttcctatcaa cttgctcagt tttgggtctg
      601 cagggtttat ctccagttcg tacaaggaga ctttagagtg tctcatgtcg agaatacttt
      661 tcggatgcga ttgcttgagt attctcgggg gataatcgct gtctccccgc atgattctta
      721 ttctctcgta aggaggcttc ggcagttcaa tcaaaccgaa cttttccttc tgcgctctct
      781 caattgcttt ccgggcgcct gcttttatcc tttttctggc ttcggcagga tgaagatgga
      841 tagctgactt gttgtgctcc ctgtatacct cttttggcag gtggtttccg gctgtcgtct
      901 gggttcccct cttcactgcg accgtttcta ttcccgggac gagcgcctgc gcttccccgc
      961 agaaggcttc gcatcctgtg gcgaaaattg ttctaatatc ctgctgtccg gcagtcaaaa
     1021 cgagatctcc gaattctcca actatcagcc cgtttatagt ctgttccaca acgctgaaat
     1081 tgccggagtg agaaaggtgt ccccctattg ttccggccat tggatgttgt cccacaaagg
     1141 caaggaaatc aattttttct ttggctatga aaagagatac tccatatcgc gaaagttcca
     1201 ctttttcatg aagaagttca ggattaacgg ctcccgagcc gtggccgtca gcaatcagaa
     1261 tttccgttgc tcctccttcc aggaaacctt ccaccgcggc gttaatctct tttgaaagca
     1321 gctcttttgc attttcgtag taacggcttt tttcagaaca ccagttgccg taatccatga
     1381 tgcctgcaac gccttccaga tctgtcataa tgtatacttt catctgttct accccgtttt
     1441 cagtatggtg tttgtagcga gtccaattta tgggcttttt taataccttg ctataaatcg
     1501 cttgtgccgg gttcatcccg ggcttgtccg gggctattcc ggtattt
//
LOCUS       DAOQXQ010000490         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_101062_length_1544_cov_2.613835, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000490 DAOQXQ010000000
VERSION     DAOQXQ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101062_length_1544_cov_2.613835"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKH81_07870"
     CDS             complement(<1..397)
                     /locus_tag="PKH81_07870"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ97993.1"
                     /translation="MRNTDVITIDDFDELYTTFGPMVLRRCRYLLRDEEAALDAMQDV
                     FVRVIERHGKLKGVCASLFYTVATSVCLNKIRSDKVRRGPRIDHILHEIADNKTARHE
                     EQTDTSLFLDLLFDGAQEDTKYMATLHYVD"
     gene            complement(547..>1544)
                     /locus_tag="PKH81_07875"
     CDS             complement(547..>1544)
                     /locus_tag="PKH81_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ97994.1"
                     /translation="LREKPASHAGAIRATAGCTLVAGADKDREALTAFGTQWKLPPQA
                     LFTDPAQMLKEIEPDLLSIAADSESHIALLDLCLEHKVPLIILEKPVAETAAEAQEVL
                     TRVEQAERAGTSRVVVNHERRFARDYRKLQELLENRTFGRLVSINARLYMGKTKTPAR
                     VLWHDGTHLVDIISFLIGTWQIEGVHGDVDSTDTPFCAIGKATGGDTPLVILDVSPGH
                     DHLCFELEFNLEKGRIRCGNGVWEVWKSEPSPYYEQFRSLRLLSPLQRSFRGKTRYFA
                     NMMAHAAALARDPSMKNESSFADGARALAILDRIISLSREATGTKGSGDVHTTGD"
BASE COUNT          330 a          397 c          431 g          386 t
ORIGIN      
        1 cgtccacgta atgaagagtc gccatgtact tggtgtcttc ctgggcgccg tcgaacaaaa
       61 ggtcaagaaa cagggaggta tccgtttgct cctcgtgtct ggccgttttg ttgtcggcaa
      121 tctcgtgcag gatatggtca atgcgtggac cgcggcgaac cttgtcggaa cggatcttgt
      181 taaggcatac cgatgtcgca accgtataaa acaggcttgc gcatacgcct tttagcttcc
      241 cgtgccgctc aattacccgg acaaacacat cttgcatggc atcaagggcc gcctcctcgt
      301 ctcggaggag gtatcggcag cgcctgagca ccatgggacc aaaagtggta taaagctcat
      361 caaagtcgtc gatggtgatg acgtcagtgt ttcgcattgt tccttccctg catacgccct
      421 ttagaacacc ctttttggac aattgtgtta ccgggggtat aaactatttt actgatcttg
      481 attgtaacaa agttaataag gaagaaaaag ggggaaggat gagctctgat atagctacgg
      541 cagggtctag tcgcccgttg tgtgcacgtc gccagagcct ttggttcctg ttgcctcacg
      601 ggagaggctg ataatccggt ccaggattgc cagggcccgc gctccatcgg caaagcttga
      661 ctcgtttttc atggaaggat cgcgggcaag ggctgcggca tgggccatca tgttggcaaa
      721 gtaccgtgtt tttccccgga acgagcgctg taagggagat agcaggcgta atgaacgaaa
      781 ttgctcgtag tacggggatg gttcactctt ccatacttcc cacaccccgt tcccgcatct
      841 gattcgccct ttttccagat tgaattcaag ctcaaaacag aggtgatcgt gtccgggaga
      901 cacatcaagg ataacaaggg gcgtgtcccc gccggttgcc tttccaatgg cgcaaaaagg
      961 agtatccgtg ctatccacat cgccgtgaac gccttctatc tgccaggtac caattaaaaa
     1021 ggagatgata tctaccaggt gggtgccatc gtgccacagg acgcgtgcgg gagtttttgt
     1081 ttttcccatg tagaggcggg cgtttataga gacaagccga ccaaaggtcc tgttctcaag
     1141 aagctcttgc aatttccggt aatcccgggc aaaccggcgt tcgtggttta cgacgaccct
     1201 gcttgtaccc gctcgttcgg cttgttctac ccgagtaagc acttcttgtg cttccgccgc
     1261 ggtttcggct accggctttt caaggataat gagcggaacc ttgtgttcaa gacatagatc
     1321 aagcagggca atgtggctct cgctatccgc ggcaatggag agcaggtcgg gctctatttc
     1381 cttgagcatc tgtgcggggt cggtaaacag ggcttggggt gggagtttcc attgtgtacc
     1441 aaaggccgtt agggcttccc tgtctttgtc cgcgccagcg accagagtgc atccagccgt
     1501 tgcccgtatg gctccggcgt gacttgccgg cttttcgcgt agcc
//
LOCUS       DAOQXQ010000491         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_101318_length_1542_cov_1.862139, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000491 DAOQXQ010000000
VERSION     DAOQXQ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101318_length_1542_cov_1.862139"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKH81_07880"
     CDS             <1..255
                     /locus_tag="PKH81_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97995.1"
                     /translation="IACFVSGVAVTLFWLFFVHAKESIPLGLCAMMFSKPSLFPSLAN
                     VDPIIAALPVSSLVFLLVNARTPPVEADVTEKAFNGIIDA"
     gene            450..>1542
                     /locus_tag="PKH81_07885"
     CDS             450..>1542
                     /locus_tag="PKH81_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNQ97996.1"
                     /translation="MIEATGAEIVVRLLEKHGIDLVAGIPGGSVLPLYEELGKSTIRH
                     ILVRHEQAGGFLAQGIARSTGKTAVCIATSGPGAMNLLTAIADARCDSVPLVAITGQV
                     NTALIGTDAFQEADTFGLSFPITKHSVMVSSARELLEAIPRAFQIAQEGRPGPVLVDI
                     PRDIQLQKVSFESWPQPIQVEKKAVRFRTDGEAFARRIDEAARALLAAKQPVLYIGGG
                     CNSSEAAEGIATVRKELPLPVVTSLMGIGAVDAEDPLLAGMIGMHGSMCANRAMYESD
                     LVMAVGVRFDDRATGVIARFCPSARIIHIDIDAAEVNKIMSAAIAIISDAESALPAIA
                     ERVREVRREKGTINDSALHDWNTRIRSLKA"
BASE COUNT          345 a          341 c          437 g          419 t
ORIGIN      
        1 atagcctgtt ttgtctccgg tgtcgcagtt accctgtttt ggctattctt tgtccatgcc
       61 aaagaatcaa ttccccttgg actgtgtgcc atgatgttct caaagccatc gctgtttccc
      121 tcgcttgcta acgtagatcc tattattgcg gccctgccgg tgtcctcctt ggtgttttta
      181 cttgtcaacg cgaggacgcc accggtagaa gctgatgtta cggaaaaagc gtttaatgga
      241 atcattgatg cttgacaata tgcataatta tgcataatat gtgaatatga atacagttat
      301 cggcctggtt gtagtccttc ttctcgtcgt cgtactcgtc gtagtacctg catgggggga
      361 aagtctgtcg cgggcatgag tgttcgtata acagagtggc cctccggaaa tccggagggc
      421 cttttttttt actattttgg aggcaagaaa tgattgaagc taccggagcg gaaattgtgg
      481 tgcggttgct ggaaaagcat ggaatcgacc ttgttgcggg aatacccgga ggttcggtac
      541 ttcctttata tgaggaatta gggaaaagca cgatacggca catccttgtc cgtcatgaac
      601 aggctggtgg atttttggcc cagggtattg cgcgctctac cggcaaaact gcggtgtgta
      661 tcgcgacaag cggccctggt gccatgaatt tgcttaccgc gattgccgat gcccgctgtg
      721 attctgtacc ccttgtagcg attaccgggc aggtaaatac ggcactgatt ggtaccgatg
      781 cgtttcagga agctgacacc tttggtcttt cttttccgat taccaaacac agtgtaatgg
      841 tcagttctgc ccgggaattg ttggaagcta ttccccgagc gtttcaaatt gcgcaggaag
      901 gtcgtccggg gcctgtgttg gttgatattc cccgggacat tcagcttcag aaggttagtt
      961 ttgagtcatg gccacagccg attcaggtag aaaaaaaggc tgtcaggttt cgcaccgatg
     1021 gagaagcctt tgcccggagg attgatgaag ccgccagggc cttacttgcc gccaagcaac
     1081 cggtactgta tataggcggt ggatgtaata gcagcgaagc cgctgaagga atcgccactg
     1141 tgcgaaagga actgccctta ccggtagtaa ccagtttaat gggtattggc gctgtagatg
     1201 cagaagatcc actgttggcc ggcatgatcg gtatgcacgg gagtatgtgc gcaaaccggg
     1261 ctatgtatga gtcagacctg gttatggcgg ttggtgttcg ctttgatgat cgggcgaccg
     1321 gggtaatcgc acgtttttgt ccctctgcca gaattattca tattgatatt gacgcggctg
     1381 aggtgaataa gataatgagc gcggcaattg cgattattag cgacgcagaa tccgctttgc
     1441 cggcgattgc cgagcgagtg cgggaagtgc gcagggaaaa ggggaccatc aacgatagtg
     1501 ccttacacga ttggaatact cgtattcgtt cgttaaaagc cg
//
LOCUS       DAOQXQ010000492         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_101557_length_1539_cov_3.518868, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000492 DAOQXQ010000000
VERSION     DAOQXQ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101557_length_1539_cov_3.518868"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKH81_07890"
     CDS             <1..1018
                     /locus_tag="PKH81_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97997.1"
                     /translation="YTFSGQSWPKARVLLVEDNELNQEVAVRLLRKASIEADIAPNGF
                     EAVRAVSRHPYDLILMDIQMPGMDGYEASKKIRKHCPTVPIIALSAAVLDEDRRRVFD
                     SGMNGHIGKPIDSSELLRVLHRFLTSPTKGSVPDDSPITDWTHADLPGFSVPQGLHAL
                     AGDELFYRKILLMFRDQLDREYKDVESILSGDNPVEAKRCIHTLKGLAGTVAANDIHA
                     IATEIDYALSQNMHVSETHIRALQQALDVARSSLAQLSNIVDDTSVSHKIGKEALEAM
                     RQSLMGNHPVDDDLLSQVISYLERIFGPGSVKDLATMVDEFEYDKAMKELADLEKRIK
                     ESDR"
     gene            1015..>1539
                     /locus_tag="PKH81_07895"
     CDS             1015..>1539
                     /locus_tag="PKH81_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ97998.1"
                     /translation="MSWKQGKKPEILIVDDQPLNLQLLSRVLSEEFDLRVARSGQKAL
                     EIAFGEDIPDLVLLDINMPDIDGYEVCKLLKGDVRTQSIAIIFITARTSVEDEEKGFL
                     LGAADYITKPFSPVVVRARVRNHINLKIRTDILESVAHTDSLTAVSNRRSYDEYLPDM
                     WNHCMRSGSSLSVLM"
BASE COUNT          417 a          310 c          384 g          428 t
ORIGIN      
        1 gtataccttt agcggacaat cttggcccaa ggcgagggtg cttcttgtag aggataatga
       61 attaaatcag gaagttgcgg ttcgattgtt acgaaaggca agcattgaag cagatattgc
      121 cccaaatgga tttgaggcgg ttcgtgcagt ctcgcgccat ccttatgatc ttatcttgat
      181 ggatattcag atgcctggta tggatggcta tgaagcatcc aaaaaaattc gcaagcattg
      241 cccaaccgtt ccaattatcg ctttgtctgc agctgttctt gacgaggata gaaggcgggt
      301 ctttgattcc ggaatgaatg ggcatattgg aaaaccaatc gacagctcag agcttttacg
      361 ggtcttacat cgttttttaa cctctccaac aaaaggttcg gtaccggatg actccccgat
      421 aacagattgg actcatgccg atctgcctgg attttcagta ccccaggggc ttcatgctct
      481 tgcaggagat gagctttttt atcgtaagat actgttgatg tttcgcgatc agcttgaccg
      541 ggaatataag gatgtcgaga gtattctttc tggcgataat ccagtagagg caaaacgttg
      601 tatccatact cttaagggcc ttgccggaac ggtggcagct aacgatattc atgcaattgc
      661 cactgaaatt gattacgccc tttcccagaa tatgcatgta agtgaaacgc acatacgtgc
      721 tttacagcaa gctcttgatg ttgcccgaag ttctcttgca cagttgagta atatagttga
      781 tgacacatcg gtatcgcaca aaattggaaa agaagcactt gaagcgatgc gccaatccct
      841 gatgggtaat catccggtgg acgatgacct tctgtcgcag gtaatttcct atcttgagcg
      901 tatattcgga ccgggaagtg tgaaagatct ggcgacgatg gttgatgaat ttgagtatga
      961 taaggcgatg aaggaacttg cggatcttga gaaacggata aaggagagtg atcgatgagc
     1021 tggaaacaag gcaaaaagcc ggagatattg atagtggacg atcaacccct aaatctgcaa
     1081 ctcctttccc gggttctctc cgaggaattt gatttgcggg tagcacgatc cggtcaaaaa
     1141 gccctcgaga ttgcctttgg agaagatatt cctgatttag ttcttcttga tatcaacatg
     1201 ccagatattg atggctatga agtgtgcaag ttactgaagg gggatgtgcg aactcaaagc
     1261 atcgccataa tatttatcac cgcgcgcaca agtgtagagg atgaagaaaa gggtttttta
     1321 cttggagcgg cagattatat taccaaaccg tttagcccgg ttgtggtgcg cgctcgtgtg
     1381 agaaatcata ttaaccttaa aattcgcact gatattcttg aatcagtagc tcacaccgat
     1441 tcactaactg ctgtttccaa tcgacgatcg tacgatgagt atcttccgga tatgtggaat
     1501 cattgtatgc gtagtggtag ttcgctaagc gttctaatg
//
LOCUS       DAOQXQ010000493         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_101896_length_1536_cov_3.282242, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000493 DAOQXQ010000000
VERSION     DAOQXQ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101896_length_1536_cov_3.282242"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKH81_07900"
     CDS             <1..930
                     /locus_tag="PKH81_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ97999.1"
                     /translation="RERERLGKSGLLGALLWAVALGTLLLIPSRPVPPMDQEFLPVVL
                     RLTQQPSEPARASPKKEAPETRQTPVTKEPRATRSPSSPARVKPVATAPDKTSSGLGI
                     PDFDQPLSNSPRGTTSGSVASSGISDTQTRPRNIEPVHEFEGAIATISPRQSSEAVVS
                     RSGSGATSASATSETLESLSSISKGASEATTNQSSKATSTRTVSATAVSQDSSSPISF
                     EGTPRRLISPADPSITLPDHLARQIDGDRSVTVMFTVRPDGSVPSSLITFSPGALLPS
                     DVRDYLRQEFSRWRFERGQDNGQARFVYSISVK"
     gene            927..>1536
                     /locus_tag="PKH81_07905"
     CDS             927..>1536
                     /locus_tag="PKH81_07905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ98000.1"
                     /translation="MIRDIDAVAFDIDGTLYPNLALYLRLIPFFMHNRRLMSEFGQVR
                     REIRRWQHEHPQEVHQDFMLWQAQLLSRRLGCTAKEAGALLDERIYKGWIPVFESISL
                     YPGVRETFAAFRAASLKIGILSDFLPSQKGELWGLPFFCDVVLGSEETGAVKPSIIPF
                     RVLAERLGCEASRILYVGNSPRSDIDGARKAGMKSALITGPFG"
BASE COUNT          329 a          437 c          387 g          383 t
ORIGIN      
        1 cgggaaagag agcgtcttgg aaaaagcgga ctccttgggg ctttgctttg ggcggtcgct
       61 cttggtactc tgcttttgat accctcccgg ccagtgcctc caatggacca ggagtttttg
      121 ccggtagtcc ttcggttaac ccaacagcca agcgaaccag caagagcttc cccaaaaaag
      181 gaagcgccag agactcgtca aacccccgtg accaaagagc ccagggcaac ccgatctcct
      241 tcatctcctg ccagggtaaa acctgtagct actgctcccg acaagacctc gtcagggctt
      301 ggcataccgg attttgacca gcccctttcc aattcacctc gcggaactac ttcagggtca
      361 gttgcatcga gtggcatctc tgacactcaa acccgcccac gcaacataga gccggtacat
      421 gaatttgaag gggcaatcgc caccatttcg cccaggcaat cctcagaggc ggtggtttcc
      481 cgatccgggt cgggtgcaac aagcgcttcg gcaacatccg aaaccctgga aagcctttcc
      541 tctattagta agggtgccag tgaggcaacg acgaatcaat cgtcaaaggc cacgtctaca
      601 cgtacggttt cggcaaccgc ggtcagtcaa gattcttcgt ccccgattag ttttgagggt
      661 accccgcgcc gtcttatcag ccctgccgat ccttcgataa ccttacccga ccacctggcc
      721 cgtcagattg acggggaccg aagtgttacc gttatgttta ccgttcgacc cgacggctcc
      781 gttccttctt cacttataac cttttctcct ggcgccctgc ttccttccga tgttcgcgat
      841 tacctcaggc aggaattctc tcgttggcgg tttgaacggg gtcaagataa tggacaggcc
      901 cgatttgtct atagtataag tgtcaaatga tacgagatat tgacgctgtt gcctttgata
      961 ttgacggaac cctctatccg aaccttgcct tataccttcg cctgataccc ttttttatgc
     1021 acaatcgccg gctcatgagc gaatttggcc aggttcgccg cgaaatccgc cgctggcaac
     1081 acgagcatcc gcaagaggta catcaggact ttatgctctg gcaagcccag ttgctttccc
     1141 gtcgcctcgg ttgcacggct aaagaggccg gcgcgcttct tgatgagcgc atttataagg
     1201 gatggatacc tgtgtttgaa tcaatatccc tgtaccccgg tgttcgggaa acctttgctg
     1261 cctttcgcgc cgcatccctt aaaatcggga ttttatctga tttcttgccc tcccaaaaag
     1321 gtgagctgtg ggggcttccc tttttttgtg atgtggtact tggctcggag gaaacagggg
     1381 cggttaagcc gtcaataatt cccttcaggg tgttagccga gcgccttggc tgcgaagctt
     1441 cgcgaatcct gtatgttggc aatagtcccc gctcggatat agatggtgca cgtaaggcgg
     1501 gaatgaagag cgctcttata actggacctt ttggga
//
LOCUS       DAOQXQ010000494         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_101997_length_1535_cov_3.257432, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000494 DAOQXQ010000000
VERSION     DAOQXQ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101997_length_1535_cov_3.257432"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..803
                     /locus_tag="PKH81_07910"
     CDS             42..803
                     /locus_tag="PKH81_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98001.1"
                     /translation="MKMRTLLVIMLLSLTLSFVSAQDQEKPYMLYVSVGGGIAGQYHE
                     QYSPLALYGPSLRIEGGWAIRNDFFLWNGQLSYNTETLSSSRGMGFEVESEMLGIQTS
                     LVALLPVMPFKTTIGLGVSMDCVGLFTENFYKYEIDVYNALFAFLGPSLFAENKILDR
                     WTFRLQCSVPLAGIQWSPVWVNGSGGINKTTLIVIPDFLYVDGRLSALLRLSERTELL
                     CMYTCIIESHKKPFDSRLFNNFLSLGLEIDLGATK"
     gene            800..>1535
                     /locus_tag="PKH81_07915"
     CDS             800..>1535
                     /locus_tag="PKH81_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ98002.1"
                     /translation="MKKQFMPFLIIFALTILGSSCSLILNEDPSDSPLEVYDSFWNVL
                     NRDYAGFYTKTYQDWPALRLTYRDTIINNPNDETLLSACTDIINQLNDGHMILFTGTQ
                     EITVYPDYTTIPFDLSVIQKTYLSQERWDASGSICTGFLPDGSYYMHVSRFSGEGWGK
                     NFDTELDRFVPGGFLIIDLRHNGGGNSEIGEDLLAQFVGESVQYGRDILHLGSDTTPL
                     NRELWIHPAQGAPRQHRVVLLINETSA"
BASE COUNT          400 a          357 c          338 g          440 t
ORIGIN      
        1 gatatactta gggtgttata tacaaaaagg aaaaagtatc tatgaaaatg cgcaccctac
       61 tggttattat gttgctttct ctgactctat cttttgtctc ggcccaggat caagagaagc
      121 catacatgct ctacgtaagc gtgggaggtg gaatcgccgg ccaatatcat gaacagtatt
      181 caccgctggc cttgtatggt ccttcgttac gaatagaggg cggatgggct attcgcaatg
      241 acttttttct gtggaacggc cagctaagct ataacactga aactctttcc agttcgagag
      301 gtatgggctt tgaagttgaa tcggagatgc ttggcattca aacatcactc gtagctctcc
      361 tgcctgtaat gccttttaag acaactatcg gtctgggtgt ttctatggat tgcgttggat
      421 tatttaccga gaacttttat aaatacgaaa tagatgtata caacgccctc tttgcatttc
      481 ttggcccgtc actctttgcc gagaacaaaa ttttggaccg ttggacgttt cgactacaat
      541 gctcagtccc ccttgctggc atccaatgga gcccggtatg ggtaaatgga tccggtggta
      601 taaataaaac tacattgatc gtaattcctg actttcttta tgttgacggg cgcctttcgg
      661 cactcttgcg attaagcgag agaaccgagt tgctgtgcat gtacacctgc attatcgaaa
      721 gccataaaaa accatttgac tcaaggctat ttaacaactt tctgagcctt ggcctggaaa
      781 ttgaccttgg agcgacaaaa tgaaaaaaca gtttatgccc ttcctgatta tattcgctct
      841 taccattctg ggaagctcct gctcgcttat tcttaacgaa gatccttctg attcaccact
      901 tgaagtatat gactcgtttt ggaatgtact taaccgggat tacgcaggct tttataccaa
      961 aacataccag gactggcctg ctcttcgcct tacgtatcgc gacactatca tcaacaatcc
     1021 taacgacgaa acgcttcttt cggcttgtac tgatattatc aaccagctta acgatgggca
     1081 catgatttta tttaccggca cgcaggagat aacggtgtat cccgattaca cgacgattcc
     1141 ctttgacctc tccgttattc aaaaaacata tctgtcgcag gaaagatggg atgcctctgg
     1201 ttctatctgc acgggatttc ttccggacgg atcgtactat atgcatgtct cccgtttttc
     1261 cggcgaggga tggggaaaaa actttgacac agagcttgat cgattcgttc ctggtggttt
     1321 tttgataata gatctaagac acaacggcgg cggtaattcc gaaattgggg aagatctact
     1381 cgcacagttt gtcggtgaat ctgttcagta cggcagagac atccttcatt taggaagcga
     1441 cacaacaccc ctgaatcgcg agctatggat ccatcccgca cagggcgccc ccagacaaca
     1501 tcgcgtcgtg ctattaatta acgagaccag cgcaa
//
LOCUS       DAOQXQ010000495         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_102063_length_1535_cov_1.504730, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000495 DAOQXQ010000000
VERSION     DAOQXQ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102063_length_1535_cov_1.504730"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKH81_07920"
     CDS             <1..506
                     /locus_tag="PKH81_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNQ98003.1"
                     /translation="LFHILQTRHDEILNGNEELLLEIVKRCVQAKARVVERDLRESGE
                     RMFLNLGHTFGHALESVAGFGSVTHGEAVAWGIGRAIELGIRLGITESEYKQDVFPTL
                     EAYGWCTSAIHPSLKDLSDVGNTLLQAMKGDKKKRGGTIRFVLQREINSTLVTEVPDG
                     EVLAVLS"
     gene            503..>1535
                     /locus_tag="PKH81_07925"
     CDS             503..>1535
                     /locus_tag="PKH81_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ98004.1"
                     /translation="MRVYLDWAATAPRDTDILVEYARLSAEYWANPSSRHAEGRKSRE
                     FLEDARRRCAQILKVQSNQLYFTSGGTESNHLPILSLLTRPTHGSIATSILEHPSIIE
                     QLKAMEATGWHTRVIPSTEEGHITSDAVCASLAEDTALVLVQAVNNETGAIQDTASIA
                     RALMISRNGKRKPHFHVDAVQAIGKIDFFCNEPGIDSASLSAHKIGGLPGSGLLYIAK
                     KTEAFLRGGGQEGGLRSGTEDVAAAGALALALEKAQKGPEKIRSISSVLYSILTDIPN
                     LHFVPSTREHHDPRFSPAIYQCAIDGIPGEVAVRMLDEAGCAVSTGSACSSRKNKRPV
                     LEAMRINPET"
BASE COUNT          429 a          364 c          376 g          366 t
ORIGIN      
        1 aactctttca tattctacaa actcgacacg acgagatcct caatgggaac gaagaacttc
       61 ttttggaaat agtcaaacgc tgtgtacaag caaaggcccg ggttgtagaa cgtgatttac
      121 gggaatccgg agagcgaatg ttcctgaatc ttggccatac ttttggccat gcgctggaaa
      181 gtgtcgcagg ctttggttca gtaacccatg gtgaagcagt agcttgggga attggacggg
      241 ccattgaact ggggattcgt ctcgggatta ccgagtcgga atacaagcaa gacgtattcc
      301 ccaccctgga agcatatggt tggtgtacgt ctgctattca tccctcgctc aaagacttaa
      361 gcgacgtagg gaataccctt ttacaggcca tgaaaggaga caagaaaaaa cgcggaggaa
      421 ctatccgttt tgtactccag cgagaaataa atagcaccct ggttacagaa gtccccgatg
      481 gggaggttct ggcggttctt tcatgagagt ctatcttgac tgggcagcta ccgcgccccg
      541 ggatacggat atccttgttg aatatgcacg attaagtgcg gaatactggg caaatccttc
      601 aagccgacac gcagaaggac ggaaaagccg tgagttcctg gaagatgctc gccgccgatg
      661 cgctcaaatt cttaaagttc aatcgaatca gctttatttt acctctggcg gaactgaatc
      721 aaaccatctg ccgatactct ccctgttaac ccgccctacc catggatcga ttgcaacatc
      781 tattctggaa catccatcta tcatcgagca gcttaaagcg atggaagcaa ctggttggca
      841 cacacgagta atcccttcta cagaagaagg tcatataaca agcgacgccg tttgtgcatc
      901 actagccgaa gacacggcct tagttcttgt tcaggcggtc aataatgaaa caggagcaat
      961 acaggatact gcatccattg cgcgagcgct tatgataagc agaaacggaa aacgaaaacc
     1021 gcattttcac gtggacgccg ttcaagctat aggcaaaatt gatttttttt gtaatgagcc
     1081 gggcattgat agcgcatcgc taagcgccca taaaattggt ggtttgccag gttcaggttt
     1141 attgtatatt gcaaaaaaaa cagaagcctt tctccggggt ggaggacaag aagggggcct
     1201 gagaagcgga acagaagatg ttgcagccgc cggagcatta gcccttgccc ttgaaaaagc
     1261 acaaaaggga ccagagaaga ttcgatccat ttcttctgtt ctgtattcta tattgaccga
     1321 tattcccaat ctccattttg ttccatcgac cagggaacac catgatcccc gtttttctcc
     1381 ggccatctat caatgtgcaa ttgacggtat ccccggagaa gtagcagtac gtatgctcga
     1441 cgaagcaggg tgcgccgtat ccactggttc tgcctgctca tcaagaaaaa acaagcgccc
     1501 ggtacttgaa gcgatgcgta taaatccgga aacac
//
LOCUS       DAOQXQ010000496         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_102092_length_1534_cov_3.214334, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000496 DAOQXQ010000000
VERSION     DAOQXQ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102092_length_1534_cov_3.214334"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..578
                     /locus_tag="PKH81_07930"
     CDS             150..578
                     /locus_tag="PKH81_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98005.1"
                     /translation="MKKAMFVVLLVSTLFCSLAAFAQSADEFVVQSVTGKVEREVSAG
                     KWEAVEKGMKLAPSTVINTGLNSSLTLNTGDSKVTVKAMQKGTVEKLTSAVASKKSGV
                     KIGAKATKSSAAEEGGPARTNVSTASTRASDATGDLEWAE"
     gene            596..1012
                     /locus_tag="PKH81_07935"
     CDS             596..1012
                     /locus_tag="PKH81_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98006.1"
                     /translation="MKKVCTFVCIALLTAFAVAAESKTDYTVLAAAGKVEKITATGSV
                     AITKGMALLATDEVSVGLNSRLVVKNGNKTLVVSAMQKGPLASLASSGNDKGGIKMSG
                     KATKSSAADQSGPDRTNVSTASTRAEAAESEEVDWE"
     gene            complement(1072..>1534)
                     /locus_tag="PKH81_07940"
     CDS             complement(1072..>1534)
                     /locus_tag="PKH81_07940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ98007.1"
                     /translation="MIVGNMGTPNKMDYTIMGNAVNLAARLEGVNKQYNTKGILISEH
                     TRAQIGEEFLLRRLDRVRVVGVQTPLRLYELVGFLHDATPEEMEAIEQWEQAIDLYEQ
                     KQFDKARRIFFSLAAKDPHDKVAPLYIKRCDDFIASPPDSSWDGVFNLTQK"
BASE COUNT          425 a          389 c          398 g          322 t
ORIGIN      
        1 cgaattctaa ccgccaaccc tactgtcttc cggggagggg aagaaagcag gaggaagata
       61 caatcgagga ataaacgacg gaaatgcccc cgaaggcatg aaaacttcgg agaaagcgat
      121 atacttaaag agaattgtgg aggtgaaaaa tgaagaaagc tatgtttgtt gtgcttctgg
      181 taagcaccct gttttgctca ctagccgcgt ttgcccaaag cgccgacgaa ttcgtcgtac
      241 agtcagtaac cggaaaagta gagcgtgaag tctccgccgg taaatgggaa gcggtagaga
      301 aaggcatgaa gctcgcaccc tcaaccgtca tcaatactgg actcaactcc tcgctcaccc
      361 ttaacacggg cgacagcaag gtaactgtta aggcaatgca aaaaggaacg gtagagaagc
      421 ttacgagcgc agtggcatcc aagaagtctg gcgtaaaaat tggcgccaag gcaaccaaga
      481 gctcggccgc cgaagagggc ggacctgcac gcaccaatgt gtcaaccgcg tctacccgcg
      541 caagcgacgc aacgggcgat cttgaatggg ccgaataaac cgcaggagag catatatgaa
      601 aaaagtatgc acctttgtgt gtatcgcctt gcttaccgcc tttgccgtag ctgcagaaag
      661 caagacagat tacacagtgc tcgctgcagc gggtaaggta gaaaaaatta ctgctaccgg
      721 atcagtcgca ataaccaagg gcatggccct tctggcaacc gacgaagtct cggttgggct
      781 taattcccgt ttagtggtaa aaaacgggaa caaaaccctg gtagtatcag ccatgcaaaa
      841 aggcccgctt gcaagccttg cttcctccgg gaacgacaag ggcggcatca aaatgagcgg
      901 caaggctacc aagagttccg cagccgacca atcaggacct gatcgaacca atgtgtctac
      961 tgcctcaacc agggcagagg ccgccgaatc agaagaagtt gattgggaat agttgcagga
     1021 tagtttctga gtagataata aaaaaggacc ggcgtgcgcc ggcccttttt tttatttctg
     1081 cgtcaagtta aacacaccgt cccaggatga gtcgggggga cttgcgataa aatcgtcgca
     1141 ccgttttata tacaggggcg caaccttgtc gtgcggatcc tttgcggcaa gggaaaagaa
     1201 aatccttcgt gccttgtcga actgtttttg ctcatacagg tcaatcgcct gttcccactg
     1261 ttcaatcgct tccatttctt ccggagttgc atcgtgaaga aagcccacca gctcatagag
     1321 cctcagcggt gtttgcacgc ccacaacgcg cacccggtcc agccggcgca acaggaactc
     1381 ttccccaatt tgcgcacggg tatgctcgct aatcaggata cccttggtgt tgtattgctt
     1441 atttacacct tcaaggcgag cggcaaggtt aaccgcgttc cccataatcg tataatccat
     1501 tttattggga gtacccatgt tgccaacgat catg
//
LOCUS       DAOQXQ010000497         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_103253_length_1524_cov_2.763785, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000497 DAOQXQ010000000
VERSION     DAOQXQ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103253_length_1524_cov_2.763785"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKH81_07945"
     CDS             complement(<1..704)
                     /locus_tag="PKH81_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98008.1"
                     /translation="MNTFNVTELQDGYFPTEDLYLDKKFLLLTPGVAITSELRRELRD
                     WEFLKVYSEGHLTDKMEPKKIQSMAGASTEKTDTKKEESKTEAEAIENESDESRFNQV
                     ERDYMEFQNCIAEIYTKYVTRKDLQLQYISDKVKEMCEFIRDNKRYVLRIQPSQENRD
                     KNYLVVHAMRSTVYAIVIALQLKFPIHKLIELYYAEHGEYPKTTASALVSGPSGTSPW
                     TDSNCAVGNARSDWVPG"
     gene            754..>1524
                     /locus_tag="PKH81_07950"
     CDS             754..>1524
                     /locus_tag="PKH81_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98009.1"
                     /translation="MKFKALFFTINIVLILAFLSIFFLPLFLVSSSYVLPFWRHTFYF
                     PLLALVVLAVINTIFAMNWTMLTLLEREDWPALAQYLERRVFESKRYSLRHARLLCDS
                     LVLVGDFAAIDTLEALLAQNKPRLYRRLASRFAAIAVLTGKYSRSRDICAQALQNRAF
                     LGGYPRAMLQLYQGLSLYLDRDLSAAMDVFLSLLQHTRDPLITAFVGYVLSRIAERRH
                     SLAPAALSASVDAKKRLKAQFGSKQWIRYLDREKTDMEV"
BASE COUNT          318 a          386 c          343 g          477 t
ORIGIN      
        1 cctggcaccc aatcgctccg tgcattaccg acagcacaat tgctatcagt ccaaggtgat
       61 gtgccactcg ggccactcac taacgcactc gcggtcgtct tcggatattc tccatgctca
      121 gcataataca gctcgataag cttatgtatg ggaaatttga gctgtagggc tattacaatg
      181 gcgtagaccg tagagcgcat cgcgtgaacg acaagatagt ttttgtcgcg gttttcctgc
      241 gaaggctgta tacgcaggac atatcgcttg ttatcccgta taaactcgca catctcttta
      301 accttgtcag atatgtattg gagttgcagg tcttttcggg taacgtattt agtgtaaatc
      361 tcggcgatgc agttttggaa ttccatatag tcgcgttcaa cctgattaaa gcgcgactca
      421 tcgctctcgt tctcgattgc ttcggcttcg gttttggact cttctttttt tgtgtcggtt
      481 ttttcagtag aagcgcccgc cattgattga atctttttgg gctccatctt atcggttaag
      541 tgtccctcgg agtacacctt caggaattcc caatcgcgaa gctcacgacg taattcgctc
      601 gttatggcta ctccgggcgt caggaggaga aacttcttgt caagatacaa gtcctctgtg
      661 gggaagtatc catcctgtaa ttcagtgacg ttgaaggtat tcacttgtta ttttccttgt
      721 attacaataa tactcatttc caggagcttt ttcatgaaat ttaaagcact cttcttcact
      781 atcaatatcg tcctaattct cgcttttctt tccatttttt ttcttcccct gtttttagtc
      841 tcttcttcgt atgtattacc cttttggagg cacacctttt atttcccgct tttggccctg
      901 gtagtgcttg ccgttatcaa cacgatattt gccatgaatt ggacaatgct cactcttttg
      961 gagagggaag attggcctgc tcttgcccag tatcttgaac gaagggtatt tgaatctaag
     1021 cggtattccc ttcgacatgc gcgccttctc tgcgattcgc ttgttttggt aggcgatttt
     1081 gccgctatag acactctgga ggcccttctg gcgcagaata aaccccggct gtatcggcgt
     1141 cttgcctccc gatttgccgc aatcgctgtc ctgacgggaa agtattcccg ttcacgggac
     1201 atatgcgcac aggccttaca aaatcgcgct ttcctgggag gctaccctcg cgcgatgctt
     1261 cagctatatc aaggcctttc tctgtatctg gatcgagact tatcggcagc catggatgtc
     1321 tttctttccc tgttgcagca tacccgtgac ccgctgataa ccgcctttgt tgggtatgta
     1381 ttgtcccgta tagccgagcg gcggcactcc ctcgcccctg ccgcccttag tgcttcggtg
     1441 gatgccaaaa agcggctaaa ggcacaattt ggcagcaaac agtggattcg ataccttgat
     1501 cgcgaaaaaa ctgacatgga agtt
//
LOCUS       DAOQXQ010000498         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_104273_length_1515_cov_3.731507, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000498 DAOQXQ010000000
VERSION     DAOQXQ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104273_length_1515_cov_3.731507"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKH81_07955"
     CDS             complement(<1..347)
                     /locus_tag="PKH81_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNQ98010.1"
                     /translation="MKQRLCTLIAGSLLLLFPFVHAQAVFPDQAEKGEQQRQAFIQAA
                     RSYLGAPYVSGGTTKRGMDCSGLVWRAGQDGPGLALPRTVVALSGQAERIADANRQPG
                     DLLFFNTTGRLSHV"
     gene            447..>1515
                     /locus_tag="PKH81_07960"
     CDS             447..>1515
                     /locus_tag="PKH81_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNQ98011.1"
                     /translation="MKSVTRQPWAFLDSWRGKAFFGQWPTMPEMFRISAERYGDRPCL
                     TVFEPNRVSLTYNETLAKVEQLAAWLSEKGIGRGDHVAVSGKNSPEWAVVYLGTLFAG
                     ATVVPIDWGLHDGEIENLLRVAKPKFFFVDEERYEKFSKIKVSGKVYSLSPSFPDTYV
                     YGLAAKKKIQIEAAHEEDIAAIMFTSGTTGNPKGVMLTHRNLVADCYIAQSNLDIYST
                     DVFYALLPIHHSYTMLAVFIEALSVGAEIVFGKSMAVSKMLKELREGKITMLLGVPLL
                     FNKLLAGILKGIRDKGIVVYGIMKFLMAVSYMIKKITGKNPGKKIFHSVLEKASLSTV
                     RIAISGGGPLAASVFKVYNELG"
BASE COUNT          386 a          319 c          391 g          419 t
ORIGIN      
        1 acatgggaaa gtctgcctgt tgtattaaaa aaaagaagat ctcctggttg ccggtttgcg
       61 tccgcgattc gctctgcctg gccagaaagg gcgaccacgg tgcggggtag ggcaagtcct
      121 ggcccgtcct gtcctgctcg ccagaccagt cctgaacaat ccatgcctcg cttggtggtt
      181 ccccccgaaa catagggggc gccaaggtat gatcgggccg cctgtataaa ggcttggcgt
      241 tgttgttctc ccttttctgc ttgatcggga aagactgcct gtgcatgtac aaagggaaat
      301 aaaagcagga gtgagccggc tatcagggtg cataatcgtt gtttcatagt aaaagtatcg
      361 gcgtattgca accgttgcct tgaattcagg gtcataattt gaatgatatc tttgttttca
      421 ttttatttcc agaacaaggg gttgatatga agagtgtaac caggcagcca tgggcatttc
      481 ttgattcttg gagaggaaag gctttttttg gccaatggcc aacgatgccc gagatgttca
      541 gaatttcggc cgagcgatat ggagatcgcc cctgtttaac cgtatttgaa ccaaaccgtg
      601 tttcattaac gtataacgaa acccttgcaa aggtagagca gcttgccgcg tggcttagtg
      661 aaaagggtat tggccgaggc gatcatgtgg cggtatcggg aaagaactct cctgaatggg
      721 cagtagtcta tttgggcact ctttttgccg gggctactgt ggttcccatt gattggggcc
      781 ttcatgacgg tgaaattgaa aacttgctcc gggttgcaaa acccaagttc ttctttgtag
      841 acgaagagcg atacgagaaa ttcagcaaga taaaggtttc tggaaaggtg tattcattaa
      901 gtccgtcatt ccccgacacc tatgtatatg gtcttgccgc gaagaaaaag atacagatcg
      961 aagcggcgca cgaagaagat attgcggcta ttatgtttac ctcgggcaca acgggaaatc
     1021 caaaaggggt aatgcttact catcggaatc ttgtagcaga ttgctatatc gcccaatcga
     1081 atcttgatat ttactcaacc gatgtatttt acgcactcct gccgattcat cattcctata
     1141 ctatgcttgc tgtttttata gaagctctgt ccgttggcgc cgagattgtg ttcggaaaga
     1201 gtatggctgt atccaagatg cttaaagagc ttcgcgaggg aaagattacg atgcttcttg
     1261 gcgtgcccct cttgtttaac aaactccttg ccggcatttt aaaaggtatt cgtgataagg
     1321 gcattgtggt gtatggcatc atgaaattcc ttatggccgt atcgtatatg ataaaaaaaa
     1381 taaccggtaa aaatcccggt aaaaaaatat ttcattcggt gttggaaaaa gcgagtcttt
     1441 ctaccgttcg tatcgcaatc tccggaggag ggcccttggc cgcaagtgtg ttcaaggttt
     1501 ataacgaact gggca
//
LOCUS       DAOQXQ010000499         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_104284_length_1515_cov_3.281507, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000499 DAOQXQ010000000
VERSION     DAOQXQ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104284_length_1515_cov_3.281507"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PKH81_07965"
     CDS             complement(<1..157)
                     /locus_tag="PKH81_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNQ98012.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGEPVVIQNSEGGRTTPSIVGFTS
                     KGERVVGQ"
     gene            complement(173..817)
                     /locus_tag="PKH81_07970"
     CDS             complement(173..817)
                     /locus_tag="PKH81_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNQ98013.1"
                     /translation="MRKNPENETEQMTNGEEVPVSAAADAATTSGQTAAPADPAQGEG
                     SQSNGAVEQSASDRIAELEKLNAELQDQYLRKAADFDNYRKRMIREKQEAIDFANTNL
                     LVDLVQTLDDFDRAISAFGEVEPGSHAASFVDGVVMIKNQMASMLEGKYGLSYYQAKG
                     ELFDPNIHEAIATNPSSEVQEPTVAEEFQKGYKLKDRVIRLSKVMVQMPAEKSE"
     gene            complement(901..>1515)
                     /gene="nudC"
                     /locus_tag="PKH81_07975"
     CDS             complement(901..>1515)
                     /gene="nudC"
                     /locus_tag="PKH81_07975"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNQ98014.1"
                     /translation="DPCGGESSGKWIRLRSIFASDDPLLASLASPAAHALGLINWHNS
                     CRFCARCGTAVIDKPGEIARVCPACSAIYYPRISPAIIVLVHKGDTVLLARHAARNQE
                     VYSCIAGFLEHGETLEECVAREVFEETGILVTDIRYAGSQSWPYPDQHMIAFHARWAS
                     GEVTVDPKEILEARWFPRDAMPATPMPGTVAWRLIFGDWGDDFR"
BASE COUNT          367 a          426 c          351 g          371 t
ORIGIN      
        1 gctgtccaac gactcgctca ccctttgagg taaaaccaac tatggaagga gttgttctgc
       61 cgccttccga gttttgaata acaacaggtt ctccgccttc cataacagac acgcatgaat
      121 tggtggttcc aaggtcaata ccgataatct ttcccatatt aatttctcct tattactcgc
      181 tcttttctgc gggcatctgt accatcacct tggacagtcg tattacccga tcctttagct
      241 tatagccctt ctgaaattcc tctgccacag tgggttcttg aacctcagat gaggggttag
      301 tcgcaatagc ttcgtgaata ttggggtcaa aaagctctcc ctttgcttga taataggaaa
      361 gcccatattt accctcaagc atagaagcca tctgattctt gatcatgaca accccgtcca
      421 caaatgatgc tgcatggctt cccggttcta cctcgccaaa ggcggaaatt gcccggtcaa
      481 agtcgtccag ggtctgcacc aaatccacaa gcaagttcgt attggcaaaa tcaattgctt
      541 cttgcttctc gcgtatcatg cgcttgcgat aattgtcaaa atccgcggcc ttgcgcagat
      601 actgatcctg aagctcagca ttcagctttt caagctccgc tatgcgatcg ctcgcgctct
      661 gttccaccgc tccattcgac tgagagcctt ctccctgagc cggatctgcc ggcgcggcgg
      721 tttgaccgct ggtagtagcg gcatctgccg cggcggaaac gggtacttct tccccgtttg
      781 tcatctgttc tgtttcattt tcagggtttt ttctcatcta taacctcgca ctgaagatac
      841 taacgagcag gcacaggggt caactgaaag tgtctctttt ttgcgcaggc gagggtttga
      901 ctagcgaaaa tcatcgcccc aatcaccaaa aataaggcgc caggccaccg ttccgggcat
      961 aggagtggca ggcatggcat cgcgggggaa ccatcgggct tccaaaatct cttttgggtc
     1021 caccgtaacc tcgccggaag cccaccgagc gtgaaaagct atcatgtgtt gatcgggata
     1081 gggccaactt tggcttcccg cataacgaat atcggtgacc aagatgccgg tttcctcaaa
     1141 cacctcccgg gccacgcatt cttccagagt ctctccgtgt tcaagaaagc ccgcaataca
     1201 ggagtacacc tcctgattcc gagcggcatg gcgtgccaaa agaaccgtat ctcccttatg
     1261 aaccaaaacg ataatcgccg gggatatacg cgggtagtaa atcgcagaac aagcaggaca
     1321 gacacgggca atctcccctg gcttgtcaat taccgccgtt ccgcaccgtg cgcaaaaacg
     1381 acaggagtta tgccagttta tcaaaccaag agcatgggcc gccggagagg caagggatgc
     1441 aagaagcgga tcatccgatg cgaatatgct tctaagacga atccactttc cagaagattc
     1501 tcccccgcaa ggatc
//
LOCUS       DAOQXQ010000500         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_104838_length_1510_cov_3.216495, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000500 DAOQXQ010000000
VERSION     DAOQXQ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104838_length_1510_cov_3.216495"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKH81_07980"
     CDS             complement(<1..579)
                     /locus_tag="PKH81_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ98015.1"
                     /translation="MKELIILSGKGGTGKTSVTAAFATLASTQGKAVFADCDVDAADL
                     HLIFSPAIKESVPFISGHVARVPTTNATILDREQGEHAQSLCRFEAISYSPETGLSID
                     EGSCEGCGVCFRFCPPGSVEFVDRRCGERYISSSRFGPVVHAALDTRAENSGKLVTAV
                     RKEAKRIAEETGAQWLIVDGSPGIGCPVIASIT"
     gene            complement(576..1436)
                     /locus_tag="PKH81_07985"
     CDS             complement(576..1436)
                     /locus_tag="PKH81_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ98016.1"
                     /translation="MILSVASGKGGTGKTTIAVSLALSSPVGVSLLDCDVEEPNSRLF
                     LGSKDWEEESVTVPVPVIDETLCTACGECARFCEFKAIVHIGKTVMVFNDLCHSCGGC
                     ALVCPVHAIHEEEEAIGSLEYSSSPVRFIQGTLSVGKAMSPPVIRAVKRKAASGAVTE
                     LTIIDSPPGTSCPMCTAVSGSDATILVTEPTPFGLHDLDLAVKTVRAMKIPFGVVINR
                     SDSGDDRVVQYCTREGIPVLMQIPEDVRIAQGYARGVPLIEAAPEYRDAFAHLVTEIM
                     SGTCFTGGAL"
BASE COUNT          440 a          419 c          333 g          318 t
ORIGIN      
        1 ggtaatggat gcaataacgg ggcatcctat accaggagag ccatctacaa taagccattg
       61 cgcaccggtt tcttccgcta tccgcttggc ttccttacga actgcggtaa caagcttacc
      121 agagttctcc gccctggtat ccagagccgc atgaacgacc ggtccaaacc gagaagaaga
      181 tatatagcgt tcaccgcatc ttctatctac aaattcaaca gaacctggag ggcaaaaacg
      241 aaaacatacg ccacagcctt cacaggagcc ttcatcaata gaaagtcccg tctccggact
      301 ataggaaatc gcttcaaacc tgcaaagcga ttgcgcgtgt tcgccttgct ctctatccaa
      361 tatagttgca ttcgttgtag gaacacgcgc tacatgacca gaaatgaacg gaacactctc
      421 tttgatggcg ggagaaaaaa tcaaatggag gtcggcggcg tcaacatcgc aatcagcaaa
      481 taccgccttc ccttgtgtgg aagcaagggt agcaaaggca gcggtaacgc ttgtctttcc
      541 tgtccctccc ttcccgctca aaataatcag ttctttcata aggcgccccc agtaaaacac
      601 gtaccgctca taatctcggt aacaagatgc gcaaaggcat cacgatattc aggagccgct
      661 tcaataaggg gaacaccccg agcataccct tgagcaatac gcacatcttc gggtatctgc
      721 attaacacag ggattccctc acgggtacaa tactgcacaa cccggtcatc accagaatcg
      781 cttcgattga taacaacccc aaacggaatc ttcatcgcac gaacagtctt aaccgcaaga
      841 tccagatcat gaagcccaaa cggtgttggc tcggtaacaa ggattgtcgc gtccgagccg
      901 gatacagcag tacacatcgg acaagaagtt ccgggaggtg aatcgataat cgtaagttca
      961 gttaccgcgc cagaagcagc tttgcgttta accgccctga ttaccggagg agacattgcc
     1021 ttccccacag aaagggttcc ttgaataaag cgtacaggcg aagaagaata ctcaagggat
     1081 ccaatcgcct cttcttcttc atgaatggcg tgtaccggac agaccagggc acagccaccg
     1141 caagaatgac agagatcgtt aaacaccata acagtctttc caatgtgaac aatggcttta
     1201 aactcgcaaa accgggcgca ctcgccacag gcggtacaca gtgtttcgtc gatcactgga
     1261 acaggaacgg taacggactc ttcttcccag tcctttgaac caagaaacag acgtgagttt
     1321 ggctcctcta catcgcagtc taaaaggctt accccaacag gagaactaag agcaagagaa
     1381 accgcaatag tcgttttccc ggtgccaccc tttcctgatg caacagaaag aatcataccc
     1441 aatgaccctc aacatcagcc tcgcgaatct gagtaagaga accttccctg taggcggcta
     1501 tcgcttgtct
//
LOCUS       DAOQXQ010000501         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_105534_length_1504_cov_3.498965, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000501 DAOQXQ010000000
VERSION     DAOQXQ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105534_length_1504_cov_3.498965"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1504
                     /gene="argS"
                     /locus_tag="PKH81_07990"
     CDS             <1..>1504
                     /gene="argS"
                     /locus_tag="PKH81_07990"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ98017.1"
                     /translation="VNVFLAKAMMSNTILSSILEAGSSYGKGTSMAGKRVMIEFSSPN
                     TNKPLHLGHLRNDALGESISRILAYSGAEVFKVNIVNNRGIHICKSMLAYSKFFAGQS
                     PESLGLKSDRFVGDCYVKYNNYSKEHPEQAEKEAQDLLLAWEAGDKEVIELWKRMNEW
                     AEQGIRQTYERTGISFDKLYYESETYLKGRDEILSGLQKGIFYQEEDGSIWVNLEPIG
                     LEKKILLRKDGTSIYITQDIGTAIYRHGDWAFNQLIYVVGNEQQYHFKVLFYVLKALG
                     YDWASDLYHLSYGMVNLPEGRMKSREGTIVDGDDLINSLRDGALEEIQAKGREEAVGD
                     PADVAEKIALAALHYFLLQVSPAKDMIFNPHESLSFQGNTGPYLQYMGARMCSILKKV
                     ESSSEYASISASPVSAELLDSEAEWELIKVLGDFGKQVERAAEGHDPSIITAYLYDLS
                     RAFSRFYHDCPILGAASPAMSRSRLELVKAARITLINAMDLVLVPFVDSM"
BASE COUNT          386 a          340 c          372 g          406 t
ORIGIN      
        1 gtgttaatgt ctttcttgcc aaggcaatga tgtccaatac aattctctcg tcaattctgg
       61 aagcgggctc ttcgtatggt aagggtacgt ctatggccgg caagcgggtt atgatcgagt
      121 tttcaagccc caataccaat aagcccctgc atctgggcca tttacgcaac gacgctcttg
      181 gcgaaagtat atcgcgtatt ctcgcttact ccggcgccga agttttcaag gtcaatatcg
      241 ttaataaccg cggaattcat atttgtaagt caatgcttgc ctattcaaag ttttttgccg
      301 ggcaaagccc tgagtccctt ggccttaaaa gcgaccgttt tgtcggggat tgttacgtaa
      361 aatacaacaa ttacagcaag gaacaccctg agcaagcaga aaaagaagcc caggatttgt
      421 tgcttgcctg ggaagccggt gacaaagaag taatagaatt gtggaagcgg atgaacgagt
      481 gggccgagca gggtatacgc cagacctacg agcgaaccgg tatttccttt gacaagctct
      541 attatgaaag cgaaacgtat ctgaagggta gggacgaaat tctctccggt ttacaaaaag
      601 gaatctttta tcaggaagaa gatggctcta tctgggtaaa ccttgaaccg attggtcttg
      661 aaaaaaagat tctgttacgg aaggacggca cttccattta tataacccag gatattggaa
      721 cggctatata ccgtcatggg gattgggcct ttaatcagct catctatgtt gttggcaacg
      781 agcaacaata tcattttaag gttttgtttt atgtacttaa ggcccttggc tacgactggg
      841 ccagcgacct ctatcatctt tcgtacggta tggtaaacct tcccgaaggc aggatgaaaa
      901 gccgcgaggg aaccattgta gacggcgatg acctgataaa tagcctccgc gatggggccc
      961 ttgaagaaat tcaggccaag gggagggaag aagcggtggg agacccggcg gatgtcgccg
     1021 aaaaaatcgc ccttgccgcc ttgcattact tccttttaca ggtgagcccc gcaaaggaca
     1081 tgatttttaa tcctcacgag tccctgtctt tccagggtaa taccggcccc tatctacaat
     1141 atatgggggc gcgtatgtgc tctattctta aaaaggttga atcatcatct gagtatgctt
     1201 ctatttctgc ttcaccggtt tccgctgaat tgctcgacag tgaagcagaa tgggagctta
     1261 taaaggttct gggagacttt ggcaaacagg tagaacgagc agcagagggc catgatccaa
     1321 gcattattac tgcctattta tacgatcttt cccgggcctt tagccggttc tatcacgatt
     1381 gtcctatttt gggagctgct tctccggcta tgtcccgctc ccgccttgaa ctggttaagg
     1441 ctgcccgcat taccctgatc aacgcgatgg accttgtatt ggttcctttt gttgattcaa
     1501 tgta
//
LOCUS       DAOQXQ010000502         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_105536_length_1504_cov_3.463078, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000502 DAOQXQ010000000
VERSION     DAOQXQ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105536_length_1504_cov_3.463078"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="proC"
                     /locus_tag="PKH81_07995"
     CDS             complement(<1..651)
                     /gene="proC"
                     /locus_tag="PKH81_07995"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNQ98018.1"
                     /translation="MNKRKVGCIGSGVMGSALMDAVISVIGSSEVAIRDSDTTKAEAF
                     AAKTGCTRLDTNRSIVESSTFVFLAVKPQYLPQVLEDISPVISEDTILVSMAAGVRIE
                     QIKKHLDRHPKIIRIMPNTPVAVGQGVIAIAPDQTVQNTDIDELARILGKAGLVELTP
                     EPLMDAVTAVSGSGPAYGYLFIEALSDAACMMGIPRQQALRYAAQTLKGAAEMVLRT"
     gene            complement(664..1458)
                     /gene="proB"
                     /locus_tag="PKH81_08000"
     CDS             complement(664..1458)
                     /gene="proB"
                     /locus_tag="PKH81_08000"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNQ98019.1"
                     /translation="MTRDLISSARKIVIKIGSNTLAKSDGSVNMAFMEGLAEQCTALM
                     KAGKQFVLVSSGAQVAGLATLDRWARKKDIHYRQALCAIGQVELMDNWRNAFAKSGTH
                     IGQLLLTGEDFKDDQRTLNIRNTLFTLVDEGVVPIINENDSVSYEEIKIGDNDNLSAL
                     TAILWSADLLILLSDIDGVYDKNPKEHPDAQLIEVVPDLHILKSTISIGSANSFGTGG
                     MQTKLEAADKATAYGIPMLLTNGGRPRFLEALADGSQNGTVFLAKD"
BASE COUNT          335 a          401 c          374 g          394 t
ORIGIN      
        1 ggtgcgaagc accatctcgg cagccccttt aagggtttgt gcggcgtaac gaagcgcttg
       61 ttgacgggga attcccatca tacaagcggc atcggagagg gcttctataa acagatatcc
      121 ataggcagga ccagagcccg acaccgcggt aaccgcgtcc atcaggggct cgggggttag
      181 ttccaccaaa ccagccttgc caagaatgcg ggcaagctcg tctatgtctg tgttttgtac
      241 ggtttgatcg ggagcaatgg ctatgacgcc ctgacccacg gcaaccggcg tattaggcat
      301 gatgcggatt atcttgggat ggcgatccag atgctttttt atctgttcaa tgcgaacacc
      361 cgctgccatg gacactaata tggtatcttc gcttattacc ggggaaatgt cttcgagcac
      421 ctggggcaga tattggggct taacagctaa aaacacaaaa gttgagcttt ccacaatact
      481 ccggtttgta tccagccggg tacagccagt ctttgcggca aaggcttccg ccttggtggt
      541 atctgaatct cgtattgcaa cctcgctcga gccaataacc gagatcaccg catccatcag
      601 ggctgagccc ataacaccgg aaccgataca gcctactttt cgtttattca ccagaaccct
      661 cccttagtct tttgccaaaa aaaccgtacc gttttggctc ccgtcggcaa gggcctccag
      721 aaatcggggg cgccctccgt ttgtcaagag catcgggata ccataggcgg tggccttgtc
      781 ggctgcttcc agtttggttt gcattccacc ggtcccaaag ctgtttgcac tgccgataga
      841 gatcgtactc ttgagtatgt gcaagtcggg tacaacttcg ataagctggg catccgggtg
      901 ttctttggga tttttatcat acaccccgtc aatatcgctg agcagtatta acagatccgc
      961 gctccacaat atggcggtaa gcgcgctcaa gttatcgtta tccccaatct ttatttcttc
     1021 gtacgagact gagtcgtttt cgttaatgat gggaacaacc ccttcatcaa ccagggtaaa
     1081 cagggtgttg cgaatattga gcgttctctg gtcatcctta aaatcctctc cggttaaaag
     1141 gagctgtccg atgtgtgttc cgcttttggc aaaggcgttg cgccagttat ccatcagttc
     1201 aacctgccca atcgcacaga gcgcctggcg ataatggatg tctttttttc gcgcccagcg
     1261 atccagggtt gcaagccctg ctacctgtgc cccggaggac accagcacaa actgctttcc
     1321 cgccttcatc aaggcggtac attgttcggc gagcccttcc ataaaggcca tgttcaccga
     1381 accatcgctt tttgccaggg tgttcgatcc tatttttatg actatcttgc gggcagaaga
     1441 gataagatcg cgtgtcatgg gcgtacctgt ccttgtcctt ctatcagaaa ttttacggtt
     1501 gtta
//
LOCUS       DAOQXQ010000503         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_105539_length_1504_cov_3.355418, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000503 DAOQXQ010000000
VERSION     DAOQXQ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105539_length_1504_cov_3.355418"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PKH81_08005"
     CDS             complement(<1..960)
                     /locus_tag="PKH81_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNQ98020.1"
                     /translation="MNKSSWVVSPAPFLLTRPTVSRIIWISMATLIPHLIMLMADGDW
                     KALLVILVSTIGALSSQAIVDLRMHREPIQYPSLLLYGILTGFLLPSTIHPIIAFGTC
                     FIGFFITRIVYGSPLASWIHPVATSIALAFISQPSLFPSNLVTPDSVRAVGDAFRALK
                     IDQFSLLPIDQGITTVINTYILSPFRIVLPDGYITLFWHSPSTIPAFRYNIFTLGASI
                     VLLSLRIIDWLLPFFFLLTYSLLVYCNSLMPFSLSPSGGDILFACLTGGILFIAFYIL
                     PEYGTLPRTRTGKAVSGILAGIIAFALCGPGGTPAGAIFTVLLI"
     gene            complement(957..>1504)
                     /locus_tag="PKH81_08010"
     CDS             complement(957..>1504)
                     /locus_tag="PKH81_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNQ98021.1"
                     /translation="KNPLVCIDAVTALSVRQAIIKNQPPLFRYVRVCGPAVVHQRILK
                     VKIGTPVGDIIEECGGFKSKPSRIVVNGLLSGMALYDLDTPITQGVKSLHLMDYDTCP
                     DFNVSDCIHCGRCLQVCPVHIDPMRVVTAIRKGKITPQLTDSLKRCQYCGCCAMVCPS
                     RIPLHHIIREGGQLVIGGNHL"
BASE COUNT          518 a          289 c          352 g          345 t
ORIGIN      
        1 aataaggagt acggtaaaaa tagcaccagc tggtgtacct ccaggtccac agagggcaaa
       61 agcaataatg ccagcaagaa taccgcttac tgcttttccg gttcgcgtgc gaggcaatgt
      121 tccatattca ggaagaatat aaaaggcaat aaaaagaata cccccggtaa ggcaggcaaa
      181 aagaatatct cctccggaag gagaaagaga aaatggcata agagaattac agtagacaag
      241 taatgaatag gttaaaagaa aaaagaatgg aagtaaccaa tcgataatgc gtaaagagag
      301 taatacaatc gaagcaccta gagtaaaaat attgtaccgg aatgcgggga ttgtcgaggg
      361 agaatgccaa aagagcgtaa tatagccatc cggtaagaca atccgaaagg gagagaggat
      421 gtaggtgtta ataactgttg taatgccttg gtcgataggc aataacgaga attgatcaat
      481 cttaagggca cggaaggcat ctcccaccgc tctgacacta tctggagtta ctaaatttga
      541 aggaaaaaga gatggttggc taatgaaggc caatgcaata cttgtcgcaa ctggatgaat
      601 ccatgatgct aaaggcgatc cgtaaacgat tcgagtaata aagaaaccta taaagcatgt
      661 tccaaaagcg attattggat gaatcgtaga gggaagtaaa aaaccagtga gaatcccata
      721 taaaagtaac gaaggatact gaattggctc ccgatgcata cgcagatcaa caatagcttg
      781 ggaactgaga gccccaatag ttgacacaag gataacaaga agcgctttcc agtcgccatc
      841 tgccatcagc ataataagat gtggaatgag cgttgccatg cttatccaga ttattcgcga
      901 gacagttgga cgagtaagta aaaaaggagc tggagataca acccaggagc ttttgttcat
      961 aaatgattac ctccgataac taactggcca ccttcccgga tgatatgatg taagggtatt
     1021 cgtgaagggc ataccatagc acaacatcca cagtattggc accttttcaa cgaatcagtc
     1081 agttgaggcg ttatcttgcc ttttcggata gcagtaacta cgcgcatagg atcgatatga
     1141 acaggacaga cttgtaaaca ccggccacag tggatgcaat cgcttacatt aaaatcggga
     1201 caggtgtcat aatccattaa atgaagagat tttactcctt gcgttattgg ggtatcaaga
     1261 tcatagagag ccataccgga aagcaaacca ttgacaacaa ttcttgatgg cttggatttg
     1321 aatcctccgc attcttcaat tatgtctcca acaggagtcc cgatttttac tttgagaata
     1381 cgctgatgaa caactgccgg tccgcatact ctaacataac ggaaaagagg aggctggttt
     1441 ttgatgattg cctgtcgtac cgaaagagcc gtaacggcat ctatgcacac caaaggattt
     1501 tttt
//
LOCUS       DAOQXQ010000504         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_105561_length_1504_cov_2.716356, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000504 DAOQXQ010000000
VERSION     DAOQXQ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105561_length_1504_cov_2.716356"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..438)
                     /locus_tag="PKH81_08015"
     CDS             complement(67..438)
                     /locus_tag="PKH81_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98022.1"
                     /translation="MIALIPLNLPFLIPVVTILMYSLIQQLCLLIFKQTAEGPTGERL
                     FLLGIVFLSLHEALSFIDALCMILSSVASLIIVTTILFSVRERLALYSIQSEWKGLPA
                     ILVSLGLCALALYGLDVSWWL"
     gene            complement(615..1193)
                     /locus_tag="PKH81_08020"
     CDS             complement(615..1193)
                     /locus_tag="PKH81_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98023.1"
                     /translation="MKKNAGLLLFALCPLIPATSRLAYAIPLAVCLLIYFFSGLICKA
                     IISQRPQGNATQALELICLGGTASVLSTILSALYPVLSISLNLYVYASAFSFIVLVSI
                     DSFSLESASKTAILLFIPLMLIFSLAREFLGYGSVSIPISTGIREFQLISTGMPIPLR
                     FWGTGAGALILAGIASWIIRLSLKKNQGRATP"
     gene            complement(1190..>1504)
                     /locus_tag="PKH81_08025"
     CDS             complement(1190..>1504)
                     /locus_tag="PKH81_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98024.1"
                     /translation="RALSDSLSIEISLVPQKKLPSMALAYTRSYRDAKTGAIYCLVSI
                     TGRAGPFPAVFSLAPAHASFISLVGLQYPKDIQRYGITEPLIHTWEQRLTAIIQQEDA
                     NQ"
BASE COUNT          540 a          291 c          336 g          337 t
ORIGIN      
        1 gaaaacaaga taatgctcgc taaaacgaca gcgatactga tgatataata cagcatgtaa
       61 tcctccctat aaccaccacg aaacgtccag gccatacaag gctagagcgc ataatcccag
      121 agaaacaagg atcgcaggga gtcccttcca ttcgctttgg atagagtaca aggctaatcg
      181 ctcacgaaca gaaaaaagta tagtagtaac tataataagc gaagctactg atgacagtat
      241 catgcatagg gcatcaataa atgatagagc ttcatgtaat gatagaaata caattcccag
      301 aagaaaaagc cgctcaccag ttggaccttc tgctgtttgc ttgaagatta gcagacacaa
      361 ctgttgaatc agagaataca taagaatagt tacaacagga atcaaaaagg gaagattcag
      421 tggtattaga gcaatcacag taactggcca taaaataaca attgagatac aaagaacaca
      481 gagaaacagg ggaatacgag aaaaaaactt aggaccatta tgggattcaa aatatgaacg
      541 ctctaatcca aaaccgtaaa aaaataaagc ggaacctgac atagcataaa aaaagagcgt
      601 taaaggaatt acattcatgg tgttgcccta ccctgatttt tttttagcga gaggcgaatt
      661 atccaagaag caatacccgc cagtatgagt gctccggcgc cagtacccca gaatcgcagg
      721 ggaatgggca tccctgtcga gattaactga aactcacgaa tcccagttga aataggaatg
      781 gatacacttc catagccaag aaattctctt gcaagagaga atatcaacat aagagggata
      841 aacaggagaa tagctgtttt agaagcactt tccagagaaa aggaatcaat actaactaac
      901 acgataaatg aaaaggcgct agcatacaca tagagattca gggatatcga taataccgga
      961 taaagtgctg agagaatcgt tgaaagaaca gatgcggttc cacccaggca gattagctca
     1021 agcgcttggg ttgcattacc ttgtggcctt tgtgaaatta ttgctttaca aatcaacccc
     1081 gaaaagaaat agatcaacaa acacacggca agggggatag cataagcaag ccgtgaggtt
     1141 gcagggataa gtggacaaag agcaaagaga agaagacctg cgtttttttt cattgattgg
     1201 cgtcctcttg ttgaataatg gcggtgaggc gttgttccca cgtatgaatc aggggttctg
     1261 taattccata acgttgaata tcttttggat attggagccc taccaacgag ataaaagaag
     1321 cgtgtgcggg tgcaagagaa aatacagcgg gaaaaggacc tgccctaccg gtaatactta
     1381 ccaaacagta tatagcccct gtctttgcat ctcggtaact gcgtgtatat gcaagggcca
     1441 tagatggtaa tttcttctgt ggtacaagag aaatctctat ggacaatgaa tctgatagtg
     1501 ccct
//
LOCUS       DAOQXQ010000505         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_106320_length_1498_cov_1.966736, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000505 DAOQXQ010000000
VERSION     DAOQXQ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106320_length_1498_cov_1.966736"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKH81_08030"
     CDS             <1..1225
                     /locus_tag="PKH81_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98025.1"
                     /translation="SGSTDPSLQETFFKEMRQAISREADRGSPLSLVCDFNGSARTLS
                     IDKEFFASLGLPFFSINDQPGHIVHRIVPEGESLTWCSNELERLRKEGPGDEERHVLF
                     GYMPDCDGDRGNIVYWDEAQHKTGILEAQEVFALSVISELSFLAHSGITENLAIAVND
                     PTSLGIEAIARSFGAEVWRAEVGEANVVNLARELRSRGKTVRILGEGSNGGNITHPSA
                     VRDPLNTMCALIKLMTIRDSGNTIGLFHRWCTLSGQENRYHEDFTFADVRASLPEFTT
                     TSVFESYAQLHITTEDHAVLKSRFQDVFIREWNTDTARIRTDWGFAGWKAIAYNGTVA
                     SDCSDNFAKSAKGGLKILFTDTDDNAVGCIWMRGSGTEPVFRILADIQGNDRTREERL
                     RSWLTSMVLEADKQI"
     gene            1260..>1498
                     /locus_tag="PKH81_08035"
     CDS             1260..>1498
                     /locus_tag="PKH81_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNQ98026.1"
                     /translation="MGIRGELFTTEVTLDNRTYFFNIKENRTGDVFLQVVESKNAEGA
                     GFDRHAIVVFEDDMQRFLQGLESSLSFIEKNRKAKV"
BASE COUNT          424 a          314 c          389 g          371 t
ORIGIN      
        1 ttctggtagc accgatcctt ccctgcaaga gacttttttt aaggaaatgc gccaagcaat
       61 ttctcgggag gcagatagag gttcaccctt atccttagta tgcgacttta atggcagcgc
      121 acgaaccctt tccatcgaca aggagttttt tgccagtctt gggctcccct ttttttctat
      181 caatgatcag ccaggacaca tagttcatcg catcgtcccg gaaggcgaaa gccttacttg
      241 gtgcagtaat gaactggagc gactgagaaa agaagggccg ggagacgaag agcggcatgt
      301 actttttggc tacatgccag actgtgacgg agaccgcggg aacatcgtct attgggatga
      361 agcgcaacac aaaaccggta ttcttgaagc tcaagaggtt tttgcacttt cggttatctc
      421 ggaattatcc tttttggccc actcgggaat aaccgaaaac ctggcaatcg cagtaaatga
      481 tccaacatct cttggtatag aggctattgc ccgctcgttt ggagccgagg tatggagggc
      541 ggaggttgga gaagcaaatg tagttaatct tgcgcgggag ttacgaagca ggggaaaaac
      601 agtaagaatc cttggagaag gctcgaacgg agggaacata acccatccaa gcgcagtacg
      661 cgatccgctt aacacaatgt gtgccctaat taaacttatg accattcgcg atagcgggaa
      721 cactatcggc ttattccatc ggtggtgcac gctttccggt caggaaaatc gataccacga
      781 ggactttacc tttgcagacg tcagggcaag cctcccagag tttactacca cctcggtctt
      841 tgaatcctat gcccagctcc acattaccac ggaagatcat gcggtgctta agagcaggtt
      901 tcaggatgtt tttattcgtg agtggaatac cgatacggca agaattcgca cagactgggg
      961 atttgccgga tggaaagcaa tagcctacaa tggaacagta gccagtgatt gctcagacaa
     1021 ttttgccaag tccgcaaagg gcggattaaa gattctattt acagatactg acgataatgc
     1081 agtcggatgt atctggatgc gaggatcagg aacagagcca gtatttcgca tccttgccga
     1141 tatacaggga aatgatagaa ccagagaaga gcggcttaga tcctggctta cgtcgatggt
     1201 acttgaggcg gacaagcaaa tatagtagta tctgagccag tatcgaagga aggttattaa
     1261 tgggaattcg cggtgagctg tttacaacag aggtaacgct tgacaacaga acgtactttt
     1321 tcaacataaa ggaaaatcgc actggcgatg tgtttttaca ggttgtagaa agtaaaaacg
     1381 cagaaggtgc aggttttgac cgtcatgcga ttgttgtttt tgaagatgat atgcagagat
     1441 ttttgcaggg actggaatca agtctttctt ttatcgaaaa gaacagaaaa gccaaggt
//
LOCUS       DAOQXQ010000506         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_106376_length_1497_cov_3.445215, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000506 DAOQXQ010000000
VERSION     DAOQXQ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106376_length_1497_cov_3.445215"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKH81_08040"
     CDS             complement(<1..560)
                     /locus_tag="PKH81_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HNQ98027.1"
                     /translation="MKNKQSGELAVCGFEAVKALADKHGEKIERLFFNQDKARFFSET
                     CKSLASRRKIYRLVEDSELEKLCKSVHHQGVVAMIANPEIPALDRQTVSSWEVSCERV
                     LVLDRVGNANNLGAIVRSAAFFGISSLVISREDDQAELSTSSWRVAQGGMEYVNIYSA
                     GSAAALLEDTAGRFVRIGADHRSSVD"
     gene            719..>1497
                     /locus_tag="PKH81_08045"
     CDS             719..>1497
                     /locus_tag="PKH81_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ98028.1"
                     /translation="MMDRTEESKPTSRRKQILDAAFFLATKSEVWSLADIAAVVGVSK
                     TAIYRHFTNRAEIEEEMTRELIEAVSTAIDTAKESPNAIRLAVVSLLRAKPGYLQYIV
                     QRIITTEGFVPIMIESLISQNERIASFFSRLERLRKGVQSHIRADLLISSVSVIIASF
                     ADDRLRLIQDQLLESLARGFPELQAVTESRLAELDKICLLEKRNEEESDRLISAIART
                     IQAHGLKNTTIENIAQEMGMAKSSLYFYCKNKNEMLASLIK"
BASE COUNT          429 a          372 c          367 g          329 t
ORIGIN      
        1 atatcgacac ttgagcgatg atcggctcca atgcggacaa aacgaccggc ggtatcttcc
       61 aaaagggctg ctgcggatcc tgcgctgtag atgttgacgt attccatacc gccttgggca
      121 acacgccaag agctggtact gagctcggcc tgatcatctt cccggctaat aaccagggac
      181 gatatgccaa aaaaggcggc gctgcgtaca atggcaccca ggttgtttgc gttccctacg
      241 cgatcgagaa ccaaaacccg ctcgcaggaa acctcccagg atgagacggt ttgccggtca
      301 agggcgggga tttcggggtt ggctatcatg gcgacaacgc cttgatgatg aacgctctta
      361 caaagctttt ccagctccga atcctctacc aaacggtaga ttttgcggcg tgaggcaagg
      421 gatttgcagg tctcgctaaa aaagcgggcc ttgtcctggt taaaaaaaag gcgttctatt
      481 ttttctccgt gcttgtcggc aagggcttta accgcctcaa agccgcacac agccagttcg
      541 ccggattgtt tgtttttcat ggcatgctcc gaagccgcag tgtatcacaa gggtaatccg
      601 gcgggaagca taaacagaaa aacaaagagt aaaaacgctt gaccaaagag cgtgaaaaaa
      661 ttatcttcag gttaccgaac gttcggtaac agatttggta cagtacggtg ttaaggatat
      721 gatggataga acagaagaga gcaaaccaac atcccgcagg aaacaaatcc tggacgcagc
      781 ctttttcctg gccaccaaaa gcgaggtctg gagcttggcg gatattgcgg ctgtcgttgg
      841 tgtatccaaa actgcaattt accgtcactt tacaaatcgc gcggagatag aagaagaaat
      901 gacgcgggag ctcatagagg cagtaagcac agccatcgac acggccaaag aaagccccaa
      961 cgcaatcaga ctcgccgttg tctcactatt gcgtgcaaaa cccggatacc ttcaatatat
     1021 cgtgcaacga ataatcacta ccgaaggctt tgtccccatc atgatcgaat cgctcatcag
     1081 tcaaaacgag cggatcgctt ccttcttctc ccggctggaa cgccttagaa agggcgtaca
     1141 aagccatata cgtgcagact tgcttatcag ctctgtttcg gtaatcatcg ccagttttgc
     1201 cgacgatcgg ctgcgcctta tacaagatca acttcttgaa tcacttgcgc ggggattccc
     1261 agagctacag gctgtaacag aaagtcgcct tgctgaattg gacaagatct gcctccttga
     1321 aaaacgaaac gaggaagaat cagaccgtct tatttcggca attgcgcgaa cgattcaagc
     1381 ccatgggtta aaaaacacca caatagaaaa cattgcccag gaaatgggaa tggcaaaaag
     1441 cagtctctat ttttattgca aaaataaaaa cgaaatgctc gcgagcctga taaagca
//
LOCUS       DAOQXQ010000507         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_106514_length_1496_cov_2.702984, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000507 DAOQXQ010000000
VERSION     DAOQXQ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106514_length_1496_cov_2.702984"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKH81_08050"
     CDS             complement(<1..1075)
                     /locus_tag="PKH81_08050"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNQ98029.1"
                     /translation="MISIAEKAVPGGYCIARPEGKAVFVTGALPGETVDIEIVEDKKK
                     YSFAKVISVLEPSNRRVTPPCPFFGVCGGCSLQMADQDFQREIRLSLLQDCLDKAAVP
                     FTGIIGFEGASGYGYRSRFQFHRDESGKIGFKHTSDDTIVPVNDCLVAQETVRKALSS
                     GYFSTIPDCPSRFHVFSSDSHVWSEPVNPEVSITIGGKDMSFDVRGFFQSNIPLFERL
                     LIDIFSRSPVITGPLLDFYSGVGTFSLAATGFCDEVVLVEHNRSACQWAQRNLDKQGT
                     TVHMCAVSDDAWPHCKQSRIPFSTAIVDPPRSGISKTALDWFIKSSVCDLRYVSCDPV
                     TFSGGAAILSNAGFHLETLTLYDF"
     gene            1114..>1496
                     /locus_tag="PKH81_08055"
     CDS             1114..>1496
                     /locus_tag="PKH81_08055"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ98030.1"
                     /translation="MITNQSIFDASDGAAITIHSWVPNTIPKGIILLAHGMAEYARRY
                     DYFADKATEQGYAVYAPDLRGHGETAGCLKNLGYLADHNGFVRVALDIGEITVHLQHM
                     NPDIPVFLMGHSFGSFISQYIIETHG"
BASE COUNT          464 a          355 c          309 g          368 t
ORIGIN      
        1 aaaaatcata gagtgtaagg gtttcaagat gaaagcctgc gttactcaat atggcagcac
       61 cccctgaaaa agtaaccgga tcgcaagaaa cataccgcag gtcacaaaca ctacttttta
      121 taaaccagtc aagcgcggtt ttggatatac cagagcgagg cgggtcaaca atcgccgttg
      181 aaaagggaat acgtgattgc ttacaatgtg gccacgcgtc atccgaaact gcgcacatat
      241 gtactgttgt tccctgttta tcaagattac gctgtgccca ttggcacgca gagcgattat
      301 gttctacaag aactacctca tcgcaaaaac ccgtagcagc aagggaaaag gttcccacgc
      361 cagaatagaa atcaagtaaa ggtcctgtaa taactggaga tctggaaaag atgtcaatca
      421 ataaacgctc aaacaatgga atatttgatt gaaaaaagcc ccgaacatca aagctcatgt
      481 cttttccgcc aattgtaata gaaacttccg gatttaccgg ctcagaccat acatgactgt
      541 ctgatgaaaa gacatgaaat cgggatggac aatccggtat agttgaaaaa tagccggaag
      601 aaagagcttt tcgtaccgtc tcttgcgcaa ccaaacagtc attgaccggt acaatggtgt
      661 cgtcagatgt atgtttaaat ccgatttttc cagactcgtc acgatgaaac tgaaaccgcg
      721 aacgataccc atatccactg gccccttcaa acccaattat tcccgtaaaa ggaacagcag
      781 ccttatcaag acaatcttgt aaaagagata gccggatttc acgctgaaaa tcctgatctg
      841 ccatctgaag gctgcaaccg ccacatacac caaaaaacgg gcagggtggg gtaacccgtc
      901 gattggaagg ctccaaaaca gagatgacct tcgcaaagga gtactttttt ttatcctcga
      961 caatctctat atcaacggtt tctccaggaa gtgcaccggt gacaaacact gctttacctt
     1021 ccggacgcgc aatacaatat ccccccggaa ccgctttctc tgctatactt atcatcgtat
     1081 gacagtatag cataaactca aggagtgcgt tttatgataa ctaatcaatc gatatttgac
     1141 gcatcagatg gggcagcaat aacaattcat tcctgggttc ctaataccat ccctaaagga
     1201 attattctgc tcgctcatgg tatggctgag tatgcgcgta gatacgatta tttcgccgat
     1261 aaggctactg aacaaggata tgccgtatat gccccggatc ttcgcgggca tggagaaaca
     1321 gccggatgcc ttaaaaacct gggatacctt gccgatcaca atggatttgt tcgcgttgcc
     1381 ttagatatag gagaaatcac cgtccattta caacacatga accccgacat tccagttttt
     1441 cttatgggac actcttttgg ctcgtttatt tcacaatata tcattgaaac acacgg
//
LOCUS       DAOQXQ010000508         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_106875_length_1493_cov_2.559110, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000508 DAOQXQ010000000
VERSION     DAOQXQ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106875_length_1493_cov_2.559110"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..>1493
                     /locus_tag="PKH81_08060"
     CDS             190..>1493
                     /locus_tag="PKH81_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ98031.1"
                     /translation="MNQLYQKGITIKSLKSYFAPYFAGMTRPTANKFFLFLLAIISIQ
                     GIQSVRFLYTWFLKRIDTSSCLNSYYYLLSEGKISRSLLNQITVRIALSCIPEECSNY
                     PIFLIIDDTLQPKYGTHFEEYQLLFDHACHNGSNYLKGHCFVGLVISVPVEVDGKLRY
                     LSVPVGYRLRSKNENKLELAAKMVEQAIQEFPTETKTILLCDSWYPKGAIRKLVSENP
                     QLELIANVRKDTKLYDLPPVPTGKRGRPAKKGKVLDYQTDFKLHTRIGDYMVGTRKVM
                     TNLFPESVYATITTFNPNNSSSYRIFISTIMPEDILLDDQELETLCPEVPEADRSRLL
                     PYCLYGFRWAIEVIFYEQKTFWSFGKYMVRSKNGIESYVNFLAIAYSCVQLLPFKQER
                     YAHLKEESSQVKKQLIGMAIQQEVFFYTFVLSIENRIKSLAIL"
BASE COUNT          450 a          303 c          293 g          447 t
ORIGIN      
        1 aaactgataa agtttgtctg tccggaaatc atctccgtac atacaaactt tctctctata
       61 tataggtcga attaaatacc accactttac aacttttcac gcccctaagg gcataaaaat
      121 acaaacaacc ccaaattttt tgtatgattg atttggataa aaaccaacaa aaaaaggggg
      181 ttgtttgcta tgaatcaatt atatcaaaaa ggcattacaa tcaaaagttt aaaatcatat
      241 ttcgctcctt attttgccgg gatgacccgg ccaacagcga ataagttttt cctttttctt
      301 ttggcgatca tttcgataca agggattcaa tccgtccgtt ttttatacac ttggtttttg
      361 aagaggatag atacctctag ctgtttgaac tcgtattact atctcctatc tgaagggaaa
      421 atatcccgat ctctattgaa tcagattact gtccgtatcg cgttgtcttg cattccggag
      481 gaatgcagta actatcccat ttttctgatt attgatgata cccttcaacc taaatacggc
      541 acacattttg aggaatacca attgctgttt gatcatgcat gccataacgg aagcaactat
      601 ctaaagggac attgtttcgt tgggttggta atcagcgtcc ctgtggaggt agatggtaaa
      661 ctgcgctacc taagcgttcc cgtcggctat cggttacgtt ccaaaaatga gaataaattg
      721 gaattagctg ctaagatggt tgaacaggca atccaagagt ttccaacgga aacgaaaact
      781 atcttgcttt gtgatagttg gtatccaaag ggggctatcc ggaagcttgt atctgaaaat
      841 cctcaattgg agttaatagc caatgtaagg aaagatacaa agctatatga cttgcctccc
      901 gtaccgaccg gtaaacgcgg gcgtcccgcc aagaaaggaa aagttttgga ttaccaaacg
      961 gactttaagt tgcatactcg gattggcgat tatatggtag gcactcgaaa agtgatgacc
     1021 aatctattcc cagaatctgt ttatgcaaca attacaacat tcaatcctaa taactcttcc
     1081 agctatcgca tttttattag cacgattatg cccgaagata ttttactcga tgaccaagaa
     1141 ctggaaacac tctgtccaga agtgcctgaa gcggacagaa gccgtctttt gccttactgt
     1201 ctatatggat ttcgttgggc aattgaagtt atcttttatg aacagaagac cttctggtcc
     1261 ttcggtaaat atatggtcag aagcaaaaat ggaattgaaa gttacgtcaa ctttttagcc
     1321 attgcctata gttgcgttca gcttcttccc ttcaaacagg agaggtatgc ccatttgaag
     1381 gaggaaagtt ctcaagtgaa gaaacaactc attggaatgg cgatccagca ggaagtattt
     1441 ttctatactt tcgtgctttc catcgaaaac aggattaaat cgctggctat cct
//
LOCUS       DAOQXQ010000509         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_107284_length_1490_cov_2.015331, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000509 DAOQXQ010000000
VERSION     DAOQXQ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107284_length_1490_cov_2.015331"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            728..>1490
                     /locus_tag="PKH81_08065"
     CDS             728..>1490
                     /locus_tag="PKH81_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ98032.1"
                     /translation="MKKAILMAFLLFVSAIAFAQEEEYYDSTPVFDAIDQRDMASLRK
                     LIKDGMYIWGKNHEGENPLMYATRVSNEQAVAVLLPCYADTSILDERNWSDKNILEIA
                     EAQNNSVIRSRIVNLAYKELKQPGNFDTLYKLVFSGNDSVRRGIEMGAIDVNSRSEDG
                     ESLLHYCASTYHPEDETKGLSMAQFLIRQGIDVNASRSDGDTALHIAIARSHTALASL
                     LIDSKADLNRIGDYSETPLSLALKHKNKEIFLKLVA"
BASE COUNT          459 a          328 c          326 g          377 t
ORIGIN      
        1 actacccggc atattggggc tatgatacaa acggaaatgt aactgcgctc gtaattgatt
       61 ttttgctact gagtctataa caagggtaac aagaatgaaa tggattccat ttctaaaaac
      121 atactttatt accttgatag tactactttt agtagactca ttactctgct atatgttggt
      181 tttttcagat ttcatattta tcaaagaggg aagcccgccg ttccctgcta tatctctcaa
      241 aatggcagat gccgtgatat ggttttttct tcacctcata ccattattgt gtctatggca
      301 gattgacata attcaagagg aactaactct ttgcataatt gccatactga cacaaaacat
      361 gcttctatct tacttactta ctttgtttat acataaaaaa aagaaacagg atcgactttt
      421 acactgatat catgacaaac tggcaggcaa tcgcaacaaa gaatctagtg tctcaaagtg
      481 ttgaaacagc ggattttgat aatgccagaa acgaatggta tgtcacagaa aaagtaatcg
      541 acagctactt atcgggatgc aacgattagg aaaatagatt atagattaga attcttacat
      601 atgaaagatc atgaatacaa gttgatagtg ccgtatctgg ataagaatca gaaggccacg
      661 tgtaataata tcagacgaga aaaggactaa aactatgaaa aaaagtttct tatacagaga
      721 taggcaattg aagaaagcga tattaatggc cttcttgctg tttgtatccg ctatagcatt
      781 cgcccaggaa gaagagtatt acgattccac gcccgtattc gacgcgatcg accaacggga
      841 catggcctcg ctgagaaagc tgatcaagga cggaatgtat atatggggta aaaaccacga
      901 aggcgaaaac ccgctcatgt acgcgacgcg cgtctcgaac gagcaggcgg ttgccgtgct
      961 ccttccctgc tacgctgaca catcgatcct cgatgaacga aattggtcag ataaaaacat
     1021 cctcgagatt gcggaagcgc agaataattc tgttatccga tcgcgtatcg taaacctggc
     1081 atacaaggaa cttaagcaac caggaaattt cgatacgctc tacaagctcg tcttttccgg
     1141 aaacgattca gtgcgacggg gtatcgaaat gggagcgatc gacgtcaata gcaggagcga
     1201 ggacggcgag tcactgctgc actattgcgc gagtacatac catcccgagg acgagacaaa
     1261 gggtctgagc atggcgcagt tccttattcg tcagggaatc gacgtcaatg cgtcgcggtc
     1321 agacggcgac acggcattac acatcgcgat cgcgcgttcc cacaccgctc tcgcgtctct
     1381 gctcatagac tcgaaggccg acctgaaccg cattggcgat tattcggaaa cgccgctctc
     1441 cctcgccttg aaacacaaga acaaagagat cttcttaaaa ctcgtggctt
//
LOCUS       DAOQXQ010000510         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_107344_length_1489_cov_3.246862, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000510 DAOQXQ010000000
VERSION     DAOQXQ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107344_length_1489_cov_3.246862"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PKH81_08070"
     CDS             <1..1381
                     /locus_tag="PKH81_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98033.1"
                     /translation="ILKSPFYRLFVVQYRNPWYTSSIMTAKKTRKSNRKTHEPVSLAS
                     RIRSLPKTVRAHPKKAALIASVPLLALLLFVVFLVMISRAPRIGIAFYRVPESLKNAV
                     IAEAEKAPLAKKPGFRIIPLEDDKSLTSQLNKNRKIALLFTEDGYAAERLAPISRVTL
                     NEDRRLMPSAMRSSGTSLFPQGKDISSYALPILIDHLELIYHKKTFEQITPPEVFSEI
                     ESRAQQIKENKGFLWALSCAGGNDEDLLSFIGAVYLSQHGDESYRLFVEALRGNPVLT
                     DLLATTKLKETLTLLTDWKAKMILHPEWYNFTRQDIEDFMWMQRAPMAVISLSHHRAI
                     ANSTLQNYDSTTFPTHSDNPDRLIIAPVYVGLRPNPRLDDVVSTDFLRSLITNNVQER
                     LASHTGFSPVNSTAQTRDKQASDLRLWVARSRRPVPNPFTASLDNPFHRTQLAKALRE
                     YLSVRGTGY"
BASE COUNT          387 a          418 c          314 g          370 t
ORIGIN      
        1 aatactaaaa tctccatttt accggctttt cgttgttcag tatcgtaacc catggtacac
       61 ttcctctatt atgacagcca aaaagacgcg aaaatccaac cggaaaacac atgaaccggt
      121 ttctctggcc tcccgtatcc gctcgcttcc caagacggtg cgtgcacacc ccaaaaaggc
      181 ggcccttatt gcctccgtcc ccctgctggc tctcctcttg ttcgttgtgt tcctggtgat
      241 gatatcgcgt gcgccacgaa ttggaatcgc cttttaccgg gtgccggaat ccctaaagaa
      301 tgcggttatc gcagaagcag aaaaagcgcc tctggcaaaa aagccaggct tccgcatcat
      361 tccccttgaa gacgacaaga gccttacaag tcaacttaac aaaaatcgta aaatagcctt
      421 gctctttaca gaagatggct atgcagccga acggcttgcg cctatatccc gcgttaccct
      481 aaacgaggat cgacggctta tgccctctgc catgcgaagc tccggtacaa gccttttccc
      541 tcaaggcaag gacatttctt cgtatgccct tcccatactt atcgatcatc ttgagcttat
      601 ttaccataaa aaaacctttg aacaaataac tcccccagag gttttttcag agatagaatc
      661 cagggcacag cagattaagg aaaacaaggg gtttctttgg gcactgagct gtgccggagg
      721 gaacgatgag gacctgcttt cctttatcgg ggccgtctat ctttctcaac atggagatga
      781 aagctaccgt ttgtttgtgg aagcactacg gggaaacccg gtgcttaccg atcttttagc
      841 aactaccaaa ctcaaggaaa cgctcacctt actgacggat tggaaagcaa agatgatcct
      901 tcatcctgaa tggtacaact ttactcgcca ggacattgag gactttatgt ggatgcaacg
      961 tgcgcccatg gcggtaatct ctttgagcca tcatcgtgca atagcgaaca gtacattgca
     1021 aaactacgac agcacgacct tccccaccca ctctgataat ccggaccgcc ttatcatcgc
     1081 gccagtttac gttgggctca ggcctaatcc tcgccttgat gatgtggtta gcaccgattt
     1141 tttacgctct cttataacga acaatgtgca agaacgactt gcttcccata ccggtttttc
     1201 tccggttaat tcaaccgcgc aaaccaggga caaacaagcc tctgatctac gcctctgggt
     1261 ggcgcgctca cgccgccccg tccccaatcc ctttactgcc tcgctcgaca atcccttcca
     1321 tcggacgcaa ctcgccaaag ctctgcgcga atatctatcc gtccgcggca caggatacta
     1381 agttaaaaaa aagagagcgg atgtgaatcc gctctcttga gcaaagaact cttgataata
     1441 ctctgttacg gaacttgtcg tctcaaatac acaatctgga agttagtgg
//
LOCUS       DAOQXQ010000511         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_108296_length_1481_cov_2.711080, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000511 DAOQXQ010000000
VERSION     DAOQXQ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108296_length_1481_cov_2.711080"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="PKH81_08075"
     CDS             complement(<1..1369)
                     /locus_tag="PKH81_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNQ98034.1"
                     /translation="MEKIFGFLETPRESVRYRRRDERVRDYREVELLRDSESASSQAS
                     RCMNCGTPFCHSACPLGNYIPEWNALVREGRWQEAWERLSLTSTFPEITGRLCPALCE
                     GACVLAYNDDSVTIRQNELDIIERAFSEGWVTPKKPVKSSDKSVAIVGSGPAALSCAQ
                     WLNRAGHKVLVFERDQKPGGFLRYGIPDFKLDKAVLDRRISLMEREGIVFECGVEIGR
                     DRDAKQLKGDFDAIVLSGGSRECRDLSIPGRELDGVLMATEYLSMSNRSVSGERSLPD
                     SFNARGKRVVVIGGGDTGSDCVGTANRQGAISVHQLELLSRPPDTRSDAQPWPQFPRI
                     FKHTSSHDEGVIQEFDVNTKAFVGRDGHLEKLECVRVSWDTSGPRPVMSEIAGSEFTL
                     EADMVILALGFTGAEKHPMLSQLGVELSPRGLVSAEADYMTNVPGIFSAGDIRRGQSL
                     IVWAFQ"
BASE COUNT          389 a          422 c          329 g          341 t
ORIGIN      
        1 cctgaaaggc ccacactatt aaagactgac ctcttctaat atctcctgca ctaaagatac
       61 caggaacatt agtcatataa tctgcttcgg cgctgacaag acctcgagga gaaagttcaa
      121 ccccaagctg ggaaagcatg ggatgctttt ccgctccggt aaagccaagg gcaagaatca
      181 ccatatctgc ctccagggta aactccgacc cggcaatttc gctcattacc ggtcttggac
      241 cgctagtgtc ccacgacacc cttacgcatt caagcttctc caggtgtccg tccctgccga
      301 caaaggcctt tgtattaaca tcaaactcct gaatgactcc ctcatcatga cttgatgtgt
      361 gtttaaaaat acggggaaac tgtggccaag gctgagcgtc gcttcttgtg tcaggcggac
      421 ggctcaacaa ttccagttga tgaacagaga tagcgccctg tctgtttgca gtgcccacac
      481 aatctgagcc ggtatcgcca cccccgatta ccacaacccg tttcccgcgg gcgttaaacg
      541 aatcgggcaa ggatctttcc cctgacacag agcgattact catggaaaga tattcagtgg
      601 ccatcaaaac accatcgagt tctcttcccg gtatcgacaa gtctcgacat tccctgctac
      661 cgccagaaag aacgatcgcg tcaaagtctc ctttaagttg cttggcatct cgatctcgac
      721 caatctcaac accacactca aatacaatgc cttctctttc cattaaacta attctgcgat
      781 caagaactgc cttgtcgagc ttaaaatcgg gaatgccata acgaagaaaa ccgcccggtt
      841 tttgatcacg ttcaaataca agcaccttat gcccggcacg attgagccat tgcgcgcacg
      901 aaagggcagc tgggcctgat ccaacaatgg cgacgctctt atccgagctc tttaccggct
      961 ttttgggggt aacccagcct tcactaaagg cacgctcgat aatatccagt tcattttgcc
     1021 tgatcgtcac actgtcatcg ttataggcaa ggacacacgc cccttcgcag agtgcgggac
     1081 aaagccggcc ggtaatttct ggaaaagtag aagtaagaga aagcctttcc catgcttcct
     1141 gccatcgtcc ttcgcgaaca agggcattcc attcagggat atagtttcca agcggacagg
     1201 ccgaatggca aaaaggagta ccacaattca tgcaacgact agcctgagag ctggcactct
     1261 cgctatcgcg gagaagctcc acctcgcgat aatcccgaac acgttcatcc cgacgccgat
     1321 agcgaacgct ctcgcgcggg gtttccaaaa agccgaagat tttttccatt agagttctcc
     1381 ctttttctca aggtaagagc gatagtcgcg cggcatgacc ttacggaaca agggtagcgc
     1441 gtgcgaaaaa tcctcaagga ttgagcgggc ttttacgctg c
//
LOCUS       DAOQXQ010000512         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_108405_length_1480_cov_3.889123, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000512 DAOQXQ010000000
VERSION     DAOQXQ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108405_length_1480_cov_3.889123"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKH81_08080"
     CDS             <1..205
                     /locus_tag="PKH81_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNQ98035.1"
                     /translation="YQNRFEMAYKGIAIASENLQAAESRIRDADMAKEMVDFTKNQIL
                     SQAGNAMIAQANTQPQSVLRLIQ"
     gene            416..778
                     /locus_tag="PKH81_08085"
     CDS             416..778
                     /locus_tag="PKH81_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNQ98036.1"
                     /translation="MSIEVASIGQQLTAMDRRLEGSVNASLRTTSQALQTTEKASDAA
                     PISEEKVSQIAQQLESISSLFDRTLKFQVSKELEQVIVKVIDSSTDKVIREIPSAEIQ
                     NLQLRIKEALGLLFDESI"
     gene            836..>1480
                     /locus_tag="PKH81_08090"
     CDS             836..>1480
                     /locus_tag="PKH81_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HNQ98037.1"
                     /translation="MSDISIPGVTASKYKTDELIQGLMKVERVPRDRAEEELKTYRSQ
                     QSAWRGVNQHAGKLRDTSRSLFSYNNPFNEKIAESTDPRAVTASANREAQDQRFRLTV
                     NQIAQADSFLSSELPSEGKVSKGRYVFNVGDSEIAFNWKGGSYREFIDALNRRGKDVL
                     RASLVQVSPTTRSLLIESLKTGANERLKFSEDALSFALESGIIKSLLPEAWLGST"
BASE COUNT          426 a          351 c          357 g          346 t
ORIGIN      
        1 ctaccagaac cggtttgaaa tggcatacaa gggaatcgct atcgcatctg aaaatctgca
       61 agccgcggaa tcacggattc gtgatgccga tatggcaaag gaaatggtcg actttaccaa
      121 gaaccagatc cttagtcagg caggaaatgc aatgatcgcc caggccaata cgcaaccgca
      181 atcggtccta cggctgatcc agtagtttcc gcagaataag ggctacacct aagagacctc
      241 tggacgtcat ctcttggata tatcccggtt tttgttggaa gaagaagaag cgcccttctt
      301 cttcctttta aaaatagccg gttttgaacc ggttcatata caatacccgt atcacggatg
      361 atacggccac gttagaaata ccgtggattg tgtttctttc aaggaggttg ccgatatgag
      421 catagaagtt gcaagcatcg gacagcagtt aacggcaatg gatcgccgtc ttgaaggttc
      481 tgtaaacgca tcattgcgaa ctacatctca ggctttacag acaacggaaa aggcctcaga
      541 cgccgctccg atcagcgaag aaaaagtatc tcaaattgcg caacagttgg aaagcatctc
      601 aagcttgttt gaccggacgt taaaattcca ggtcagtaaa gagctggagc aggtaatcgt
      661 caaggttatc gattccagca ccgataaggt tataagagaa ataccgtctg cggagataca
      721 gaatcttcag ttaaggatca aggaggcatt aggccttctc tttgacgagt cgatataacg
      781 gatgtgcact gccgtcattc cgtacctctg acagcgggcg tgagggaggt cagcattgtc
      841 cgatataagc ataccgggcg taacggcaag caaatataaa actgacgaac tcatccaggg
      901 attaatgaag gttgagcgag tcccccgcga ccgcgccgaa gaggaattaa aaacataccg
      961 gagtcagcaa tcggcatgga gaggtgtaaa ccaacatgcc gggaagctcc gcgatacctc
     1021 gagaagcctc ttttcctaca ataatccctt taacgaaaaa atcgctgaat ctactgatcc
     1081 acgtgcggtt accgcatcgg caaatagaga agcgcaagat cagcgatttc gtcttaccgt
     1141 taatcaaatt gcccaggcag acagctttct ttcctcagaa ttaccttcgg aaggcaaggt
     1201 atccaagggt cgctatgtct ttaacgttgg ggattcagag attgccttta actggaaggg
     1261 gggtagttat cgcgagttta tcgatgccct taaccgccgg ggaaaggatg tattacgcgc
     1321 atcccttgtt caagttagtc ccactacccg ctcgctttta atagaatcct taaaaaccgg
     1381 cgctaacgaa agacttaaat tctccgaaga cgcccttagc tttgccctgg aaagcggtat
     1441 tatcaaatct cttctcccag aggcgtggct cggatcaaca
//
LOCUS       DAOQXQ010000513         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_108436_length_1480_cov_2.712982, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000513 DAOQXQ010000000
VERSION     DAOQXQ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108436_length_1480_cov_2.712982"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKH81_08095"
     CDS             complement(<1..759)
                     /locus_tag="PKH81_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ98038.1"
                     /translation="MKKSVSMAVFAVIAACALWAGGAKDAGAASGKITKDNIKVGFVY
                     IGTIDDEGYTQAHDQGRLALNAMGIATAYIEHVAENADCEKAIRDLIDQGCNVVYTNS
                     FGYMDWTIKVAKDHPDVFFGHCSGYKRAANVSTYFGKIYQARYLSGIAAGKKTKVNRI
                     GYVAAMPIPEVIRGINAFTLGIQSVNPKATVEVIWTNTWFDPAVEKQAALELLNKGCD
                     VIAQHQDTTAPQIAAQEKGVFAIGYNSPTASAAPK"
     gene            complement(805..1386)
                     /locus_tag="PKH81_08100"
     CDS             complement(805..1386)
                     /locus_tag="PKH81_08100"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNQ98039.1"
                     /translation="MRLLQDRIRKDGIVLPGNILKVGSFLNHQLDVALFNEIGREFAR
                     RFAGTPVSRILTIEASGIAIACITAQYFSNVPVVFAKKAAGANLSGDFYSTPVSSYTH
                     GNTYTMTVSREYLQQGDHVLLIDDFLANGCALEGLAALVRQAGATVSGAGIVIEKGFQ
                     NGGERLRAAGVRVESLAVIDAMSQEEGVVFRRT"
BASE COUNT          381 a          391 c          359 g          349 t
ORIGIN      
        1 cttgggagcg gctgatgcag tgggagagtt atacccgatg gcaaacacac ccttttcctg
       61 tgcggcaatc tggggagcag ttgtatcttg atgctgggca ataacatcgc agcccttgtt
      121 caaaagctcc agggcagctt gcttttctac agctggatca aaccaggtgt tagtccagat
      181 aacctctacg gttgccttgg ggtttaccga ttgaatgcca agggtaaagg cattgatgcc
      241 ccggattact tccggaattg gcatagcggc aacatagccg atgcgattaa ccttggtctt
      301 tttccctgca gcgataccgg agaggtagcg agcctgatag atctttccaa aataggtaga
      361 cacattggca gcacgcttgt aaccggagca gtggccaaag aatacatcgg gatggtcctt
      421 ggcaaccttg attgtccagt ccatgtagcc aaagctgttg gtatacacta cgttgcagcc
      481 ctgatcaata agatcccgaa tcgccttttc gcagtcggcg ttttccgcaa cgtgctcgat
      541 ataggcggtg gcaatcccca ttgcgtttaa ggcaagacga ccctgatcgt gtgcctgggt
      601 gtaaccttcg tcgtcgatgg ttccgatgta gacaaaaccg accttgatgt tgtccttggt
      661 gattttaccg ctagcagcac ccgcatcctt tgcgccgccg gcccacaggg cacatgctgc
      721 gataacggca aaaaccgcca tgcttacgct ctttttcatt tccttctcct ctatattaaa
      781 aaaaaactac ccgattagaa gcgcttaggt gcgtcgaaac acaacccctt cttcctgact
      841 catcgcatct atgaccgcaa gggattccac tcgtacaccg gcagcgcgaa ggcgctcccc
      901 gccattttga aagccttttt ctattacaat gcccgcgccg ctcaccgtag ctcctgcctg
      961 acgaaccagg gcggcaaggc cctcaagggc gcatccattg gcaagaaaat cgtcaataag
     1021 caacacatga tcgccttgtt gcaggtactc acgagaaacc gtcatggtat aggtattacc
     1081 gtgtgtatag gaagaaaccg gggtagaata aaaatccccg ctcaaatttg ctccggcggc
     1141 ttttttggca aacacaactg gtacattgga aaaatactgg gcggtaatgc acgcgatggc
     1201 aataccggaa gcctcgatgg taagaatacg ggatacggga gtgccggcaa acctgcgggc
     1261 aaactcgcgg cctatctcgt tgaacagggc aacatcaagc tggtgattca agaaacttcc
     1321 aaccttaaga atattaccgg gaagaactat cccgtccttc cgtatcctgt cctgcagtaa
     1381 ccgcatctat aaccccttta tgattgcttt gataattgca tctgcgcccc gagctgccaa
     1441 cggtgaacac ggagaaaaaa accgtaaaaa agtaaaaaga
//
LOCUS       DAOQXQ010000514         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109009_length_1475_cov_3.317606, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000514 DAOQXQ010000000
VERSION     DAOQXQ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109009_length_1475_cov_3.317606"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            785..>1475
                     /locus_tag="PKH81_08105"
     CDS             785..>1475
                     /locus_tag="PKH81_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNQ98040.1"
                     /translation="MSTHMQSYVITKKSWKLFGRISIVWNAIQGKRLVYLVAILSLAI
                     EVLFTFTSPFIVKITIDSIIGKEPLSIPLFLNQGIIEWLPTREWFLTRLWVPALAFVG
                     CILFQSLFSFIAGYSANVCAEHAAKRLRDRLYAHIQNLPYETLLRSQSGDWFQRCTSD
                     VDTVRRFICSESMEIFRTLFLVSVAFPVMYSLSPRLTFWGILVVPLIILYSCGFHVIV
                     ERVFIKADEKEG"
BASE COUNT          411 a          279 c          334 g          451 t
ORIGIN      
        1 cactttctgg taagcgattc aagatctgcc cagatgccac gattaacact ggggtgttga
       61 ggagagatat tgctgtaata gaaacaatcc ttcatagctt gagcggagta tcgcatgtcg
      121 gccgcagggg ttagatgtcc tcggtctaaa ccggagcctt tataatctga atcgctagca
      181 gtgtcgtctg gaaaaaaggg atctggataa aatcgagttg cgcgggatat agccttagtt
      241 gttgcctctg tttttgataa agaccaaccc acccacagtg attgtcgggt tgcaatatta
      301 aagcaaagct gatggccttc gtatacatga tagttgcaag gataggctgt aaatggttct
      361 tcgagattgc tttgatttgc aaaaagaagt cgtagaatga taaaacaaag acctgtcaat
      421 gagttcggta agaaatacat atatagagta tatcacaaga aatgttaacc aacaataaca
      481 taaaaaatgc ttgacacagt gtatggggaa ggataggatt tgctatactt ttaaaaagga
      541 ggaagggata tgtactattt cgcacgaata cagatgcatg tcatggcgaa taaaagcggg
      601 atacctgtat ccaagtatcc attttgtggc gcatatccaa tcctttccgg gctaatttac
      661 taaacccgta tatacagaaa gatagaggat cggcgccaag ggttgccgat cttttttttt
      721 gttctggatt cctgacaaac aaagacggtg acataacccc tggaatactt cctggaggaa
      781 gctgttgtct acacatatgc aatcttatgt aatcacaaaa aagtcctgga aattatttgg
      841 tcgaatttcg atagtatgga atgctattca aggaaaaaga ctggtgtatc tagttgcgat
      901 actatctctg gccatagagg ttctattcac ctttacctct ccatttattg tcaagataac
      961 cattgatagt attattggaa aggaaccgct ttcgattcct ttatttctga atcaaggaat
     1021 catagaatgg ttgccaacaa gagagtggtt tttgactcgt ttgtgggttc ccgccttggc
     1081 gtttgtgggt tgtattctgt ttcaatccct tttcagcttt atagccggct atagcgccaa
     1141 tgtctgtgcc gagcatgctg ccaagaggct acgagaccgc ttatacgcac atattcaaaa
     1201 tcttccctat gaaaccctat tacggtccca aagtggagac tggtttcaac gatgtacctc
     1261 ggatgttgat accgttcgtc gctttatttg tagcgagagt atggaaattt ttcgcactct
     1321 gtttcttgtt tctgttgcct ttcccgtaat gtactccctt agtcctcgcc ttaccttttg
     1381 gggaatttta gttgttcctc taattattct gtactcatgt ggctttcatg taatagttga
     1441 aagagtgttt atcaaagcgg atgagaagga agggg
//
LOCUS       DAOQXQ010000515         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109101_length_1475_cov_1.617606, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000515 DAOQXQ010000000
VERSION     DAOQXQ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109101_length_1475_cov_1.617606"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKH81_08110"
     CDS             complement(<1..1108)
                     /locus_tag="PKH81_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNQ98041.1"
                     /translation="MAVKRFQNLLKTRNFGFIIGFLFSVFFCLTGFSSGLFGDLEAKL
                     LDTYFSVKSLGSRNAIQKGVWKADANDAVSPDILILGIEAKTLQRFGNWPFPRGVHAD
                     LVNSLSRIKNQEEREKSLFLDIFFIDETQNPADDQKLADSISQSGRVFLESILDASSY
                     GEAHDREMLYRQNFLLEKTGSFAHIGDGWKTMRPFQGIQTNLPRFNRAAKGYGAANVV
                     QDGDDKLVRRQPLVFKYSLLAGELVYEELDEEFTVDEAAYERLSWYDREGKEHQISLP
                     LHKAELADLKTVLWDQAPPLVYDSDGDGEAETETRVVEIYRDFFIPSITFSLALDYWG
                     SSLSDCRVYPGSHIEVSRPSAPPVGRRRRRGGRWP"
     gene            complement(1122..>1475)
                     /locus_tag="PKH81_08115"
     CDS             complement(1122..>1475)
                     /locus_tag="PKH81_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ98042.1"
                     /translation="AAEIAYSRKEWQVAREYYLSAAKAAPKAYTAGIALFNAAVSAEE
                     SGDEDGALALFTQAANTKGFGLVPRALFNAARIEEQRSQKDAALALYTRLVSEFADDS
                     WGKLAKSRILSLESN"
BASE COUNT          440 a          351 c          292 g          392 t
ORIGIN      
        1 gaggccagcg tccgccacgt cttcttctcc gcccaaccgg aggagctgaa ggacgggata
       61 cctcaatatg tgaaccagga tagaccctac aatcagagag gcttgaaccc cagtaatcaa
      121 gagccaggga gaaggtaatc gatggaataa agaaatcacg atagatttca actacacgcg
      181 tttcggtttc tgcctcacca tcaccgtcag aatcatagac taaaggaggg gcttgatccc
      241 ataaaacagt cttaagatca gcaagttctg ctttatgtaa gggtaacgaa atttgatgtt
      301 ccttaccctc tctatcatac caggacaacc gctcatacgc agcttcatct acagtaaatt
      361 cttcgtcaag ttcttcatac acaagctctc cggccagcaa tgaatacttg aacactaatg
      421 gttgacgacg aacaagttta tcatccccat cctgaactac atttgcagct ccgtaccctt
      481 ttgccgctcg attgaaccga ggaagatttg tctgaatacc ctgaaagggg cgcattgttt
      541 tccaaccatc gccgatatgt gcaaaagaac ctgttttttc cagtaaaaaa ttctggcgat
      601 acaacatttc tctatcatgt gcttcaccat aggaagatgc atcaagaatc gattcaagaa
      661 ataccctgcc cgattgagaa atactatcag ctagtttttg atcatcagct ggattttggg
      721 tttcatcaat aaagaaaata tccaggaaaa gagatttttc ccgttcttcc tgattcttga
      781 ttcttgataa agaattgaca aggtctgcat gtactccccg gggaaagggc caatttccaa
      841 aacgctgtaa tgtttttgct tctataccaa gaataaggat atcaggagag acggcatcat
      901 ttgcatccgc tttccatacg cctttttgga tagcatttcg tgaaccaagg ctttttaccg
      961 aaaaataggt atcaaggagc ttagcttcaa gatcgccaaa caggccggaa gaaaaaccgg
     1021 tcagacaaaa gaaaacagaa aacaaaaaac cgataataaa accaaagttt ctggtcttaa
     1081 gcagattctg gaatctcttt acggccatag ctcctcccgt attagttact ttccagcgat
     1141 aatatccttg atttagctaa ttttccccat gaatcgtctg caaactcgct tactaatctg
     1201 gtatataagg caagggccgc atctttttgt gaacgctgct cttcaattct cgcagcatta
     1261 aataatgctc tgggaaccaa tccaaaacct tttgtgttag ccgcttgggt aaacaaggca
     1321 agcgccccat cttcatcgcc ggattcttca gcgcttaccg ctgcgttaaa aagagcaata
     1381 cctgcggtat aggctttggg agcagctttt gcagctgaaa gataatactc acgagctacc
     1441 tgccattcct tacgggagta agcaatttca gcagc
//
LOCUS       DAOQXQ010000516         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109671_length_1470_cov_2.494700, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000516 DAOQXQ010000000
VERSION     DAOQXQ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109671_length_1470_cov_2.494700"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="gdhA"
                     /locus_tag="PKH81_08120"
     CDS             complement(<1..950)
                     /gene="gdhA"
                     /locus_tag="PKH81_08120"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNQ98043.1"
                     /translation="MANAYISEVLASVKARNGHEGEFLQAVDEVLVSLEPVVNQMPEI
                     QKHSILERMVEPERVIMFRIPWVNDAGKVCVNRGYRVQFNSAIGPYKGGLRFHPSVNL
                     SILKFLGFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDGEVMRFCQSLMTELYRHIGAD
                     TDVPAGDIGVGGREIGYMFGQYKRLSNEFTGVLTGKGLAYGGSLIRPEATGYGCVYFA
                     ENMLAVKGDGFKGKVCSVSGSGNVAQYAVEKLNELGAKVVTLSDSDGFIYDADGIDAE
                     KLAYVMEIKNVKRARISEYVKKYSKATYTAGERPWSVKVD"
     gene            complement(1155..>1470)
                     /locus_tag="PKH81_08125"
     CDS             complement(1155..>1470)
                     /locus_tag="PKH81_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98044.1"
                     /translation="PSENMATMRAINPPDEIKDILKKNGLGNNGFEKIIVITAAFGVV
                     EMEEAFAGQNEQMQDNPEMQSYMDEAMKQLDELKASVHKDDFALVTKRRADIAGVLSA
                     SE"
BASE COUNT          364 a          394 c          328 g          384 t
ORIGIN      
        1 caatcaacct taacagacca aggcctttct cctgcagtat aggtagcctt gctgtatttt
       61 ttgacgtact cgctaatgcg ggcgcgcttt acgttcttga tctccataac ataggcgagc
      121 ttttcggcgt cgataccgtc cgcgtcgtag ataaagccat ctgagtcaga aagggtaacc
      181 accttggcac caagctcgtt gagcttttca accgcgtact gggcaacgtt accggaaccg
      241 gaaacagagc acaccttacc cttgaatccg tctcccttaa cggcgagcat gttctcggca
      301 aagtatacac aaccataacc ggtagcctcg ggacgaatga gcgagccacc gtatgcaaga
      361 ccctttccgg ttaatactcc ggtaaactcg ttggagaggc gcttatactg accaaacata
      421 taaccaattt cgcgacctcc tacgccgata tctccggcgg gtacgtcggt atccgcgccg
      481 atatggcgat agagttcagt catcaggctc tggcaaaagc gcattacctc gccgtcgctt
      541 tttcccttgg gatcaaaatc ggaaccaccc ttaccaccac ccatagggag tgtggtcaag
      601 ctgttcttga atacctgctc aaaaccaaga aatttaagaa ttgaaagatt aacggaagga
      661 tggaagcgca agccgccctt gtaaggtcca atagcgctat tgaactgaac gcgatatcca
      721 cggttaacac atacctttcc ggcatcgttt acccagggga tgcggaacat gataacccgt
      781 tcgggctcaa ccatccgctc aaggattgaa tgtttctgga tttccggcat ttgattaaca
      841 accggctcaa ggcttaccag aacttcgtct actgcctgaa ggaactctcc ctcatgaccg
      901 tttcttgcct taacagaggc aagcacttca gaaatgtacg cattcgccat ttttcttact
      961 cctcattgag caatccggcc gcggtccgcg cccggtctgc cacctttggc atctttataa
     1021 gcttattgag ggaaatacta tcaccaggct aggaggagtg tacacacaaa aactgcccga
     1081 aactgatgaa atttcgggca ggcttgtata aaaatgcagt ttttttgtat atcgaacgta
     1141 taaaaaagag gcgtttattc gctcgcgctt aagaccccgg cgatatcggc gcgtcgtttg
     1201 gttaccaggg caaaatcatc cttatgaaca cttgctttaa gttcatcaag ctgcttcatc
     1261 gcttcgtcca tgtacgactg catctcgggg ttatcctgca tttgctcgtt ttgtccggca
     1321 aaggcttctt ccatttctac cacaccaaag gctgcggtta taacgataat tttctcgaac
     1381 ccgttgttcc caagtccatt cttcttgagg atgtctttaa tctcgtccgg aggattgata
     1441 gcgcgcatcg tcgccatgtt ttcggacggg
//
LOCUS       DAOQXQ010000517         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109731_length_1469_cov_3.419378, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000517 DAOQXQ010000000
VERSION     DAOQXQ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109731_length_1469_cov_3.419378"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKH81_08130"
     CDS             complement(<1..846)
                     /locus_tag="PKH81_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNQ98045.1"
                     /translation="MKKLCLATLALILSWALSAATITVSVIDRDLDIPLEGATISLQG
                     FPEKTVTGSDGIANLELPPDFKRGLLITELPGYLGKRITVTADQQKVSVALSITDVIE
                     GKELVVERTVPGKTDEKAGVSVVMNKDDMKTTANFGMVEDVMSSIMTLPGVGFSGGWN
                     AQPSIRGGYPEEMGCVLDDVYIINPFHWGGAFSIFNPNMVETAKMSHGIFSSRYGRAM
                     SGLLEVTTVKPKSDEVRFNVGISTISADAFAQVPLGQKASVFLGGKVTYLETLQILYD
                     DIIGYK"
     gene            complement(862..1392)
                     /locus_tag="PKH81_08135"
     CDS             complement(862..1392)
                     /locus_tag="PKH81_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98046.1"
                     /translation="MDVGVYLDTDSNLFLQYQGGTDLFYTTFLMGFDTQWSWDWDHQT
                     ETFQLGFGIGNRFQLSRRLSLDLELIGRWIFDVTEENPYMDKVRENEEIVIETEEDWD
                     EFYDEFYAGFIPSLRLSFNVAIARHFALFTAVNLDFRFLDIDRRAFEIGKHSEPIKLD
                     KEELWVYPSLSFGVKF"
BASE COUNT          381 a          368 c          356 g          364 t
ORIGIN      
        1 cttgtagcca ataatgtcgt cgtaaagaat ctgcagcgtt tcaagatagg taacctttcc
       61 accaaggaat acgcttgcct tttggccaag cggcacttgc gcaaaggcgt ctgccgatat
      121 agtggagata cccacgttaa agcgaacctc atccgatttt ggttttactg tagtaacttc
      181 caaaagtccg gacatagccc gtccatatcg agaggaaaaa ataccgtggc tcatcttggc
      241 ggtttctacc atgttcggat taaagatgga gaaggcccca ccccaatgga atggatttat
      301 aatgtacacg tcgtcaagta cgcagcccat ttcctcggga tagccgccgc gaatggacgg
      361 ttgcgcattc cacccgccgg agaagccaac gccgggaagg gtcataatgg aagacataac
      421 gtcttctacc atgccgaagt tcgcggttgt tttcatgtcg tccttattca taacgacgga
      481 tacacccgct ttctcgtcgg tctttccggg aacagtacgc tcaacaacca gttcctttcc
      541 ttcgataaca tcggtaatgg aaagggctac cgataccttt tgctggtcgg cggttacggt
      601 aatgcgttta cccaggtatc cgggtaattc ggtaatcaag agtccccgct taaaatcagg
      661 aggaagttca aggttagcta ttccatcgga tccagtaacg gtcttttccg gaaacccttg
      721 caaggagata gttgcccctt ccagcggtat gtcaagatcg cggtcgataa ctgatacagt
      781 tattgtcgcc gcgctaagcg cccaggaaag gatgagcgcc aaggtggcaa ggcaaagttt
      841 tttcatcgat atcccctgtt attaaaattt tacgccaaag gacagcgagg ggtataccca
      901 cagctcttcc ttgtcaagtt tgatcggttc ggaatgcttt ccgatttcaa atgcgcgtcg
      961 gtctatgtcc aggaatctaa agtccagatt cactgcggta aagagcgcaa agtgtcgtgc
     1021 tatggcaacg ttaaaggaaa gtctgagcga cgggataaac cctgcgtaga actcgtcgta
     1081 gaactcatcc cagtcttcct cggtttcgat aacgatttct tcgttttcgc gcaccttgtc
     1141 catatacgga ttctcttctg ttacgtcaaa gatccatcgg ccaataagct ccaggtccag
     1201 ggagaggcga cgggagagtt ggaaccggtt tccgatacca aagccaagct ggaaggtctc
     1261 tgtttgatga tcccaatccc agctccattg agtgtcaaat cccataagga aggtagtgta
     1321 aaaaagatca gttccgccct ggtattgcaa aaagaggtta ctgtcggtat caaggtaaac
     1381 cccaacatcc aacacgccgt cctggataaa gttaagaaga cccagggcga gcccgaaaca
     1441 attccgggct cgccctgggt cttcttaac
//
LOCUS       DAOQXQ010000518         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109903_length_1468_cov_2.242038, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000518 DAOQXQ010000000
VERSION     DAOQXQ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109903_length_1468_cov_2.242038"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH81_08140"
     CDS             <1..795
                     /locus_tag="PKH81_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98047.1"
                     /translation="NFGKLLTDKNFWGAVENTFVMWGWNFAPQLGIALLLSIWLSDIR
                     LKLKGKGFFRAVIYMPNLLTAASVALLYRSLFGYPLGPVNQFLQSLDIYSESIRDGEV
                     LREAFNFFRNVTMSRAIVAFIQWWMWYGHTVIMLMAGITSISASLYESAVVDGANSRQ
                     TTWYITLPMLRPMMLYILVTSMIGGMQMLEVPFLLTDMRGAPDFKIRTTNVYVYNLAF
                     QGVNDYAYAAAISIGVFVITITLALIIFLFLQDRSDLKKAKKEASV"
     gene            792..>1468
                     /locus_tag="PKH81_08145"
     CDS             792..>1468
                     /locus_tag="PKH81_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ98048.1"
                     /translation="MKSYNNALNIKRFFVYAFMIFLAILAIIPIYLLLVNATRNNAQI
                     NEGISLIPGGNTMFNWRTLTSRNFQIQQGFTNSFFIAVTTTIVSVYFSAMTAYGLHIY
                     RFKGRNYLWGLILFVMMLPPSLGFIGFYQFVAKLRLLDNYIPLIIPTIAAAGTVLFLR
                     QYLSSVLSLDLIDAARIDGAGEFRTFNTIIFPIITPALAAQSIFTFVGSWNNFFTPFV
                     LISSVKK"
BASE COUNT          339 a          351 c          298 g          480 t
ORIGIN      
        1 aatttcggaa agcttcttac tgacaagaac ttttggggag cagttgagaa tacctttgtt
       61 atgtggggtt ggaactttgc ccctcagctt ggaattgccc ttctgctttc aatttggctc
      121 tctgatattc gtcttaaact taaaggaaaa ggctttttca gggccgttat ctatatgcct
      181 aacctgctta cggcggcatc agttgccttg ctgtatcgca gtctctttgg atatcccctg
      241 ggtccggtta accagttcct ccagtccctg gatatctatt ctgaatccat ccgcgacggt
      301 gaagttctcc gcgaagcatt taactttttc cgtaacgtaa ccatgtcgcg agcaatcgtt
      361 gcatttatcc aatggtggat gtggtacggt catacggtta ttatgctcat ggcaggtatt
      421 accagtattt ccgcttcgct gtatgaatct gcagttgtag acggcgccaa tagccgacag
      481 accacttggt atattaccct tcctatgctt aggcccatga tgttgtacat cctggtaacc
      541 tccatgatcg gtggtatgca gatgctcgaa gtccccttcc ttcttaccga tatgcgtggt
      601 gcgcctgact ttaagatccg aaccacaaac gtatatgtat ataaccttgc cttccagggc
      661 gttaatgatt atgcgtatgc cgcggctatt tccatcggcg tatttgtgat caccatcacc
      721 ctggcactga ttatattcct ctttcttcaa gatcgaagtg atctaaagaa agcgaaaaag
      781 gaggcaagtg tatgaaatcc tacaacaatg ccctgaacat caagcgtttt tttgtatacg
      841 cctttatgat ctttcttgcc attttggcaa ttattcccat atacctattg cttgttaacg
      901 cgaccagaaa caacgcgcaa atcaacgaag gtatttctct tattcccggt gggaatacca
      961 tgtttaactg gagaacgctt acaagccgca atttccagat tcaacagggt tttaccaaca
     1021 gcttctttat cgccgtaact acaaccattg tgtctgtgta tttctccgca atgacggcat
     1081 atggactcca tatttaccga tttaagggac gcaattatct ttggggtctt atcctctttg
     1141 ttatgatgct tcctccttct ctcggcttta tcggattcta tcagttcgtg gccaagctta
     1201 gactcttgga taactacata ccgcttatta ttccaacgat tgcggcagca ggtacggtat
     1261 tgtttttgcg acagtatctt tcctctgtcc tctctcttga tttgattgac gcggcacgta
     1321 ttgacggagc tggtgagttc agaactttta acactattat tttcccgatt attactcccg
     1381 ctttagcggc gcaaagtatc tttaccttcg tcggttcctg gaataacttc tttactccct
     1441 ttgtacttat ctcgagcgta aagaaata
//
LOCUS       DAOQXQ010000519         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_109904_length_1468_cov_2.212314, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000519 DAOQXQ010000000
VERSION     DAOQXQ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109904_length_1468_cov_2.212314"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PKH81_08150"
     CDS             complement(<1..176)
                     /locus_tag="PKH81_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ98049.1"
                     /translation="MANNAIVNMFRIKELRERIIFTITILAVFRLGSVLTIPGIDPNA
                     LTTFFKSQVKGNAFV"
     gene            complement(198..671)
                     /gene="rplO"
                     /locus_tag="PKH81_08155"
     CDS             complement(198..671)
                     /gene="rplO"
                     /locus_tag="PKH81_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ98050.1"
                     /translation="MSDFNLYAPEGANKKKRIVGRGSSSGRGTTAGRGNKGQQSRSGG
                     KVYVGFEGGQMPLYRRIAKRGFSNYPFRVDYEVFNLHQLETRYTDGETVNRESLVIKG
                     LLKKVSAPIKVLGEGDFTRKVTVSVDKVSASAKEKIEKAGGTVVQADGTDTRTAQ"
     gene            complement(671..856)
                     /gene="rpmD"
                     /locus_tag="PKH81_08160"
     CDS             complement(671..856)
                     /gene="rpmD"
                     /locus_tag="PKH81_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNQ98051.1"
                     /translation="MANRICIKLVRSTIGQKPKVVATVRSLGLKKLNSTVEHDTTDPV
                     LGMVNAVKHLVEVKEIK"
     gene            complement(862..1377)
                     /gene="rpsE"
                     /locus_tag="PKH81_08165"
     CDS             complement(862..1377)
                     /gene="rpsE"
                     /locus_tag="PKH81_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ98052.1"
                     /translation="MDHHKDFNKEREPKEFVEKLVKLNRTAKVVKGGRRFSFSALTVV
                     GDQKGRVGFGFGKANDVSEAIRKSIDKAKRNMVHVPIKNGTIPHEIQASFKSSEVLLR
                     PACSGTGLIAGGPVRAVLEAAGATDVLSKSLGSSSSVNVIRATFEAIKGLMDAKTVAK
                     NRGKTLKDLWG"
     gene            complement(1386..>1468)
                     /locus_tag="PKH81_08170"
     CDS             complement(1386..>1468)
                     /locus_tag="PKH81_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ98053.1"
                     /translation="FDRNGYIYHGVVKAVADGARSAGIEF"
BASE COUNT          364 a          389 c          335 g          380 t
ORIGIN      
        1 acaaaggcgt ttcccttaac ctgactctta aaaaaagtgg tcaatgcgtt gggatcaata
       61 ccaggaatgg tcaataccga accaagacgg aataccgcga gaatggttat cgtaaagata
      121 atccgctccc gcagctcctt gattctgaac atatttacaa ttgcgttgtt tgccatgttt
      181 ctacacctgt ttaaggatta ttgagcggta cgggtatccg ttccgtcggc ctgaacaacg
      241 gttccaccgg ccttttcgat cttctccttc gcagaagcag aaaccttgtc cacagatacg
      301 gttaccttac gggtaaagtc accttctcca aggaccttaa tgggagcaga aaccttcttg
      361 agaagaccct taattaccag agattcacga ttaacggttt ctccatcggt ataacgagtc
      421 tcaagctgat gaaggttaaa cacttcataa tcaacacgga acggatagtt tgaaaatccg
      481 cgtttggcga tacggcggta taagggcatc tgaccgccct cgaagccgac gtacaccttg
      541 ccgccggaac gggactgctg tcccttgttt ccacgacctg cggtggtacc gcgacctgag
      601 gatgaacctc ggccaacgat acgttttttc ttgtttgccc cttccggtgc atacagattg
      661 aaatcagaca ttacttgatc tccttgactt caacgaggtg cttcacggca ttcaccattc
      721 ccaatacggg atcggtggta tcgtgctcaa ccgtcgaatt gagcttcttc aagcccagag
      781 aacgaactgt cgcaacgact ttcggtttct ggccaatggt gctgcgaaca agcttaatac
      841 aaattctatt tgccatttaa gttaacccca cagatccttc agggttttgc ccctgttttt
      901 ggcaacggtt ttcgcgtcca tcaaaccctt gatagcctcg aaagtagcac ggataacgtt
      961 tacggaactg cttgatccaa gactcttgga aagcacatcc gtcgctccgg ctgcctcgag
     1021 aaccgcgcga accgggccac cggcgataag accggttccc gaacaggcag ggcgtaaaag
     1081 aacttcggag ctcttaaagc tagcctgaat ttcatgggga atggttccgt tctttatggg
     1141 aacatgaacc atattgcgtt tggccttgtc gatacttttg cggatagctt cggatacgtc
     1201 gttagcctta ccaaaaccga atccaacacg gcctttctgg tcgcccacaa cagtgagcgc
     1261 ggaaaaggag aaacggcgtc cgccctttac aaccttagcg gttctgttga gctttacgag
     1321 cttctcaacg aactcttttg gttccctttc cttgttaaag tccttatggt gatccatagc
     1381 cactcctaga attcgattcc ggcgctgcgg gcaccgtcgg caacggcctt aacaacacca
     1441 tgatagatat agccgtttcg gtcgaaca
//
LOCUS       DAOQXQ010000520         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_110632_length_1462_cov_2.803838, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000520 DAOQXQ010000000
VERSION     DAOQXQ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110632_length_1462_cov_2.803838"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKH81_08175"
     CDS             <1..505
                     /locus_tag="PKH81_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ98054.1"
                     /translation="KNLDILQPSEIFGEMAILENSPRSATAIAYDDVKALEFNKANFE
                     VLMMGNPAIAMKLLKTFVKRIFAQKRRFMVLTVPEKMARVGDVFLMLDETQASIDRST
                     EFRTFTISMDEVARWAGLPVEEVEDEMHRFIDQGKMEIFDDRIVVKNITELSRYVNAR
                     RSKLTEK"
     gene            545..617
                     /locus_tag="PKH81_08180"
     tRNA            545..617
                     /locus_tag="PKH81_08180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Thr,seq:cgt)
     gene            complement(747..>1462)
                     /locus_tag="PKH81_08185"
     CDS             complement(747..>1462)
                     /locus_tag="PKH81_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNQ98055.1"
                     /translation="LILERIGIAPALEASGLQVSPEFALVNCEVSGTATQKVGKVWHA
                     WLLVKYPQKEKERMLERWNASVLTIKELKAREEKIPVQFRVSFSTADGRSQFRAGEQV
                     IFRFTSERDCHLLILDHQSDGTTVLLFPNRFHPDSFVAKGEVLLIPPANAPFRIVVGE
                     PFGDDRIEAIASTDESALHRDVAKRVRDLKGGLDFAAAERASFTRVIDSEFSGNGKDS
                     DGSKALWSRAEINFSTWPK"
BASE COUNT          380 a          374 c          350 g          358 t
ORIGIN      
        1 gaaaaacctg gatatcctcc aaccttccga aatttttggc gagatggcaa tcttggagaa
       61 ctctccccga tcggccacgg ccatagccta cgacgatgtt aaggcgcttg agttcaacaa
      121 ggcaaacttc gaggttttga tgatgggaaa ccccgccatc gctatgaagc ttcttaaaac
      181 ctttgtcaaa cgtatctttg ctcaaaaacg ccgctttatg gttctgacgg ttccggaaaa
      241 aatggcccgc gtaggcgatg tcttcttgat gctcgacgaa acccaggcca gcatagatcg
      301 atctaccgag tttagaacct ttactatcag tatggacgag gtagcacgat gggcagggct
      361 ccctgtagag gaagttgaag acgagatgca ccgctttatc gaccagggca agatggaaat
      421 ttttgacgac cgcatcgttg taaagaatat caccgagctt tcccgctatg taaacgcacg
      481 tcgcagcaag cttaccgaaa aataattgac gaggggccct ctttttgtta ctatcatcac
      541 tcacgccaag atagctcagt tggcagagca gcaccctcgt aacgtgcagg tcaagggttc
      601 gattcccttt cttggcttaa aatggaaaag gtcagtcgca agactggcct ttttctatta
      661 taggtaggca agatggggaa tcgatattac tgctcgcagg attgttggta atcttgcaga
      721 atagttttag tacaagagct ctttctttat ttaggccagg tcgagaagtt gatctcggcc
      781 ctgctccaca gcgcctttga tccgtctgag tctttaccgt tcccggaaaa ttcggaatcg
      841 ataacacgag taaacgaggc acgctctgct gcagcaaaat caagtcctcc cttaagatcg
      901 cgtacccgct tcgctacatc cctgtggagt gcgctttcgt cggtagatgc aatcgcctct
      961 atacgatcgt cgccaaaggg ctctccgaca acaatgcgaa agggtgcatt ggctggagga
     1021 ataagcaaaa cttctccttt tgcaacaaaa ctatcagggt gaaagcggtt cgggaataaa
     1081 agaacggtag tcccgtcgct ctgatgatcc agaataagca gatgacagtc gcgctcggaa
     1141 gtaaacctga agattacctg ctctcccgcc ctgaactggg atcttccatc cgcagtagaa
     1201 aaggagacgc gaaactgaac cggtatcttt tcttccctcg cctttagttc cttgatggtt
     1261 aagaccgagg cattccaccg ctccagcatc cgctcctttt ccttttgggg atattttacc
     1321 aatagccacg catgccaaac ctttccaacc ttctgggtag cggttccgga aacctcgcag
     1381 ttaacaaggg caaactctgg cgaaacctgg agtcccgaag cctcaagggc cggggcaatc
     1441 cctatacgct caagaatcag gg
//
LOCUS       DAOQXQ010000521         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_110860_length_1460_cov_3.136655, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000521 DAOQXQ010000000
VERSION     DAOQXQ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110860_length_1460_cov_3.136655"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1460)
                     /locus_tag="PKH81_08190"
     CDS             complement(<1..>1460)
                     /locus_tag="PKH81_08190"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ98056.1"
                     /translation="FDEEGISWGSSVRSEDGKILLFDGASLTALFNGKNDRYAASVAL
                     EKAVSGLSVSLDSNGIVITGVADGEYPGVVLVVTDDKGGKYKGEPVTIVVDSALPELS
                     VDTTARPYWLQNVLPVSGKATDGRGIAKVEYSLDSGLSWTALNGADFKANIDISALPD
                     GKIDVIIRATDRAGRVSYDWRVFLKDTVAPLVEAVFPAPADVVNGETRIGFRLADSSP
                     VVLGEYRAPGDRSAKDRSQYLPLELSSLTHTKVGTAEQPISDKMEFRFTDSAGNATST
                     SSWLFTVDAKADLPVVEIHLPEENEVIRKDFVISGVVYDDDQPAKVWYKIDNGPFTEV
                     LIENSYSIPVALLSLTDNEHTITLYAEDIHGVRGEQVVRKIRVSLEEPKAAVQTPSFE
                     TTNKAVIDIKGVASDKNGIDRVEISLDNGNSFNLAEGTESWSYRFDTRVIEDGTHVVF
                     IRVYDKYETIGLYSSLINIDNTAPSIRLELPLDGSR"
BASE COUNT          374 a          390 c          337 g          359 t
ORIGIN      
        1 gcggcttccg tccagaggaa gttcaagacg aattgatgga gcggtattgt ctatgttgat
       61 aagactggaa tacagaccga ttgtttcgta tttgtcgtaa acacggataa agacaacatg
      121 ggtgccgtct tcaattacgc gggtgtcaaa gcgataactc caactttccg ttccttccgc
      181 aaggttaaaa ctgttaccgt tatccaaaga aatctcgacc cggtctatac cgtttttgtc
      241 cgaggctaca cccttgatat cgataacagc cttgttggta gtttcgaacg agggcgtctg
      301 aaccgcggcc ttgggttctt ccaacgatac acgaatcttg cgaacaactt gctcgcccct
      361 gacgccatga atatcctccg cgtacagggt aatggtgtgc tcgttatcgg taagacttaa
      421 cagagctacc gggattgaat agctgttttc tatcaacacc tcggtaaaag gtccgttatc
      481 aatcttgtac catacctttg ccggctgatc atcgtcgtac acaacaccgg agataacaaa
      541 atccttacgt ataacctcgt tttcttcggg taaatgtatc tctacaacag gaaggtccgc
      601 ctttgcgtct accgtaaaga gccaacttga agtgctggtt gcgttaccgg cggaatcagt
      661 aaaccggaat tccatcttgt cggatattgg ctgctcggcc gtaccaacct tggtatgagt
      721 aagggacgac agttccagtg gcagatactg agagcgatcc tttgccgaac ggtcgccagg
      781 ggcacgatat tcacctaaga ctactggcga tgaatccgcc aggcggaacc caatccgggt
      841 ttcaccgttg accacatctg cgggagcggg gaacaccgct tctaccaggg gtgctaccgt
      901 atccttaagg aatacacgcc agtcgtacga tacgcggccc gcgcgatctg tggcacgaat
      961 aattacatca atcttcccgt caggaagtgc gctaatatct atatttgcct taaagtctgc
     1021 tccattaaga gctgtccagg aaagacctga atccagggag tattcaacct ttgcaatgcc
     1081 ccgaccgtct gtagccttgc cgcttaccgg taacacattc tgaagccaat agggtcgagc
     1141 agttgtgtca acagacaatt ccggcaacgc gctatccact acaatggtta cgggctctcc
     1201 cttgtatttc ccgcctttgt cgtcggtaac cacaagcact acgccaggat actcaccatc
     1261 ggctacgccg gtaataacaa ttccattgga atccaaggaa accgacaaac cagatactgc
     1321 cttttccaat gcaacggagg ccgcataccg atcattcttg ccgttaaaaa gggctgtaag
     1381 cgaagcgccg tcaaacagaa gtatcttgcc atcctcggat cgaaccgagc ttccccagga
     1441 tatgccttct tcatcaaaca
//
LOCUS       DAOQXQ010000522         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_111020_length_1459_cov_2.356838, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000522 DAOQXQ010000000
VERSION     DAOQXQ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111020_length_1459_cov_2.356838"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKH81_08195"
     CDS             complement(<1..1076)
                     /locus_tag="PKH81_08195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HNQ98057.1"
                     /translation="MSALDKPWMDTSKTREQRTALLVEAMTIEEKIAQMVQISYSLVT
                     EAEADQWALRGAGSFLHTLGNNARRLQRLACETRLGIPVIFGIDAIHGHAIHNGATVF
                     PSQLALAAGWNLELAEKMAEVTAKEVAAEGLHWTFSPVFCLGRDSRWGRIDETFGEDP
                     LLAGRMGAAFVRGYQGDDPAHPERIIACAKHYIAYGESTGGRDSYDTSVSLRTVRDIF
                     LPPFKEAIQAGCHTVMAGYESVDRVPCSASKKLLTDVLKKELGFEGFVVTDWSNLTSL
                     IKRQHLADNFRDASKIAIEAGNDMIMTSPEFYDAALSLVRDGTIPLSLIDESVRRIIS
                     VKFAAGIFDTPEKVKTLSIEHDPA"
     gene            1211..>1459
                     /locus_tag="PKH81_08200"
     CDS             1211..>1459
                     /locus_tag="PKH81_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ98058.1"
                     /translation="MLDKMRNIGIMAHIDAGKTTTTERILFYTGKIHRIGEIDDGDAT
                     MDWMEQEQERGITIQSAATTTWWKDHQINIIDTPGHVDF"
BASE COUNT          372 a          358 c          362 g          367 t
ORIGIN      
        1 cttgcaggat catgttcaat gctgagtgtt ttaacctttt ccggggtatc aaaaatacct
       61 gcagcaaatt ttacggaaat aattcgcctg actgattcgt caataaggga aagggggatc
      121 gtcccgtccc gaacgagaga cagggccgca tcgtagaact cgggcgaggt catgatcatg
      181 tcgtttcctg cctcgatagc tatttttgac gcgtcgcgaa agttatctgc aagatgttga
      241 cgtttaatca agctggtaag attggaccaa tcggttacaa caaagccctc aaagccaagc
      301 tcttttttta ggacatcagt gagcaatttt ttactcgctg agcagggcac ccgatcgacg
      361 ctttcgtatc cagccataac ggtgtggcat cctgcctgta tcgcttcctt aaaaggaggc
      421 aagaagatgt cccggacggt acgcaaggaa acagacgtgt cgtaggaatc ccttcctcca
      481 gtgctttctc catacgctat ataatgcttg gcgcaggcga taattcgctc tggatgggca
      541 gggtcgtcgc cctgatatcc acgaacaaag gccgccccca tacgtcctgc taaaagcgga
      601 tcttctccaa aagtttcgtc tatgcgtccc caacggctat cgcgtccgag acagaacacc
      661 ggagagaatg tccagtgtaa tccctctgcc gcgacttctt tggcggttac ctctgccatt
      721 ttttccgcaa gctccagatt ccagccggcg gcaagggcga gctgagaggg aaatacggtg
      781 gctccattat gaatcgcatg gccatgaata gcatcgatac caaagataac cgggataccc
      841 aaacgtgttt cgcaggcaag gcgttgtagg cggcgggcgt tattgcccaa ggtatgtaaa
      901 aatgagccag ccccgcgcaa ggcccattgg tccgcctcgg cttcggttac aaggctatag
      961 cttatctgca ccatttgcgc aattttttcc tctatggtca tggcctctac aagtaaggcg
     1021 gttctctgtt cccttgtttt gctcgtatcc atccagggtt tatctagtgc tgacatcttt
     1081 gacctctcgt taaaagtttc ttccagaaac aattatccct gatgcggtgc ttttttgcaa
     1141 gggtcaaatg tcgttcccct tgagaaaacc cgggtaaacc ggtagtatga aggggaatca
     1201 ggagtcagta atgctggata agatgcgcaa tattggaata atggcccaca tcgatgcggg
     1261 gaaaactact acaaccgaac gaattctttt ttataccgga aaaattcatc gaattggaga
     1321 gattgacgac ggcgatgcaa cgatggactg gatggagcag gaacaggagc gcggcattac
     1381 cattcagagc gctgcaacga ctacctggtg gaaagatcat caaataaata ttatcgatac
     1441 cccaggccac gttgatttt
//
LOCUS       DAOQXQ010000523         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112206_length_1450_cov_2.335484, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000523 DAOQXQ010000000
VERSION     DAOQXQ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112206_length_1450_cov_2.335484"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKH81_08205"
     CDS             complement(<1..818)
                     /locus_tag="PKH81_08205"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ98059.1"
                     /translation="MKVPRARTIVFSLCVCLIVCVFTLYMGMEYRQRKNELISIRLAL
                     LSSRLDATVANLSDFSRFVYETSVSRDEVTALMNRAWKASLSERSALRNELRAMMADE
                     YELLTRFNFRQLHFHFPDTTSFLRMHSPLVFGDALGDVRLSIKKANSELVPVGGFEEG
                     RIYNGFRFVYPLFYQGEHSGSVEVSFSMKNVLDILAEVSAGDLQFIISRSVVEDVVFS
                     DQLDHYRESSFSSDFMYDGDVLPGESNRALLDSLDSSFTARLAAWADFGIDTRF"
     gene            complement(875..1432)
                     /locus_tag="PKH81_08210"
     CDS             complement(875..1432)
                     /locus_tag="PKH81_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ98060.1"
                     /translation="MGGGNMKKEKTLMNRYRIAVIAIGLFILLLLIKKELAPLALASL
                     SRQVLTMFFVIPPIFLLLGLLDVWVPRETLIQFMGEGSGIRGKLIAFAIGSAAAGPLY
                     GAFPVAAVLMKKGASFTNILTFLGAWSTTKIPMLLFEMQSVGFRFALARLIIDIPGIV
                     VIAWAVKRCLGTEEIERMYEKARES"
BASE COUNT          473 a          357 c          321 g          299 t
ORIGIN      
        1 aaaaatctgg tgtcaatacc aaagtctgcc catgcagcca atcgagcagt aaaggatgaa
       61 tcaagagaat caagcaaggc tcggttggac tcgccgggaa gaacgtcccc gtcatacata
      121 aaatcggaag aaaaagaact ttcccgatag tgatccaact ggtcagaaaa aaccacatcc
      181 tccaccaccg accgggaaat aataaattga agatctccgg cgctcacttc ggctaaaata
      241 tcaagaacgt tcttcatgga aaaagaaacc tctacggagc cagaatgttc accctgataa
      301 aaaagcggat acacaaagcg gaaaccgtta tagatacgcc cctcctcaaa gccgcccacc
      361 ggaacaagtt cgctattcgc ttttttaatg gaaagccgca catccccaag ggcatcgcca
      421 aaaaccaggg gactatgcat gcgtaaaaaa ctggtagtat ccggaaagtg gaaatggagc
      481 tgtcggaaat taaagcgtgt aagtagctca tattcatctg ccatcattgc gcgaagttca
      541 ttgcgtaagg cggaacgctc cgaaagggat gccttccatg ccctgttcat taaagcggtt
      601 acctcatcgc ggcttacgct tgtctcatag acaaaccggg aaaagtccga aagatttgcg
      661 acagtcgcat caaggcggga agaaagcaag gcaagccgta tagaaataag ttcatttttc
      721 ctctggcgat attccattcc catatacagg gtaaagacac acacaataag acaaacacac
      781 agggaaaaca caatggtgcg ggctcttgga actttcatga tttatataag tgtaaccgag
      841 agcccactgg ctgtcaaaaa aaagatggtg tatctcagga ttcccgagcc ttctcgtaca
      901 ttcgttcaat ttcttctgta ccaagacacc gcttaaccgc ccaggcaatg acaacaatac
      961 ccggtatgtc gataataagg cgagcaaggg caaaccgaaa tccaaccgac tgcatttcaa
     1021 acaaaagcat cgggattttt gtggtagacc aggcacccaa aaaggtcaga atattggtaa
     1081 acgaagcacc tttcttcatc aatacggcag ctaccgggaa ggctccgtat aaggggcctg
     1141 ctgcggcgga cccgatggca aaggctataa gctttccccg gataccggac ccttcgccca
     1201 taaactgaat taaggtctcg cggggaaccc aaacatcaag taaaccaaga agaagaaaaa
     1261 tcggagggat cacaaaaaac atcgtgagta cctggcgcga cagggatgca agggcaagcg
     1321 gagcaagctc cttttttatc agcagtaaaa ggataaaaag accaattgct atcaccgcga
     1381 tccgataccg gttcataagt gtcttttctt ttttcatatt cccaccaccc atgctacaaa
     1441 aaaggctgct
//
LOCUS       DAOQXQ010000524         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112300_length_1449_cov_2.856528, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000524 DAOQXQ010000000
VERSION     DAOQXQ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112300_length_1449_cov_2.856528"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKH81_08215"
     CDS             complement(<1..429)
                     /locus_tag="PKH81_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ98061.1"
                     /translation="MDTVSIPFRDKEIECASREKIEAIQLEALRRQVEHALKTDFYSK
                     RFAKAGITGPQDIKTLSDISRLPFTTKGDLREAYPFGLLAVPQSEVVRVHASSGTTGT
                     PTVIYLTQKDLDLASDTMARSLTAAGGSKNDIIQNMMTYGL"
     gene            complement(448..1017)
                     /locus_tag="PKH81_08220"
     CDS             complement(448..1017)
                     /locus_tag="PKH81_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNQ98062.1"
                     /translation="MTYDIILAGVGGQGVLSLATIIARGAMIDGFEVRQSEVHGMSQR
                     GGGVQAHMRISDTKIHFDLIPLGGADMLLSMEPLESLRYLSFLKAEGILVTASEPFVN
                     IPDYPDTEDLYKKIRALPRSTIVHAEQIAREAGNLKAVNMALIGAASRHLPVSEASLE
                     KSVHDIFARKDPRLVEINMKAFKLGEQAQ"
     gene            complement(1014..>1449)
                     /locus_tag="PKH81_08225"
     CDS             complement(1014..>1449)
                     /locus_tag="PKH81_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HNQ98063.1"
                     /translation="VFAARASGEIPVEQLCQMMIANQKATYGDGLDGQALHPYMWLVK
                     GHYYSSDLAFYNFPYAFGQLFALALYGRSRQEGSSFAGVYEDILLDTGRMDAVKVTAR
                     AGFDIESPDFWRQGIKHFIEKIEEFERLVEGQSGRDNGGDTQ"
BASE COUNT          370 a          384 c          334 g          361 t
ORIGIN      
        1 aaggccgtag gtcatcatgt tctgaataat atcgtttttg gaaccgccag ctgcagtaag
       61 cgagcgagcc atggtgtcgc tggcgagatc caaatccttt tgcgtaaggt aaataaccgt
      121 gggagtcccg gtagtaccgc ttgaggcatg cacgcgcact acctcgcttt gcggaacagc
      181 aagtaaacca aagggatagg cttcgcgcaa atctcccttg gtggtaaagg gcaggcgact
      241 gatatcagag agtgtcttga tgtcctgcgg accggtaatg cccgccttgg caaagcgctt
      301 ggaatagaag tcagttttta gggcatgttc tacctgtcgg cgaagagctt ctagttgaat
      361 tgcctcaatt ttttccctac tcgcgcactc aatctcttta tccctgaacg gaatactcac
      421 cgtatccatc gcgtagctcc ttatttttta ttgagcctgt tctccaagct taaaggcctt
      481 catgttaatc tccacgagtc gaggatcttt gcgggcaaag atatcatgga cacttttttc
      541 caaagaggcc tcgctcaccg gaaggtggcg cgaagcggct ccaatcaagg ccatattaac
      601 cgccttcagg ttccccgcct cgcgggcaat ctgttcggca tgaacgatgg tgctcctcgg
      661 aagggctctt atctttttgt acagatcttc tgtgtcgggg taatcaggaa tattaacaaa
      721 gggttcagag gcagtaacca aaataccctc tgcctttaag aaagataggt agcgtaacga
      781 ctcaagcggt tccatggaca acagcatatc tgcgccccca agcggtatca gatcgaaatg
      841 aatctttgta tccgaaatac gcatgtgggc ttgaacccct ccgccccgct gactcattcc
      901 gtgtacctcg gactgtcgaa cctcaaatcc gtcaatcata gccccacggg caataatggt
      961 cgccagagaa agaactccct gtccaccaac tccagcaaga ataatatcgt aggtcattgt
     1021 gtatctcccc cattatcgcg gccagattgg ccttccacaa gtcgctcaaa ttcttctatc
     1081 ttttctataa agtgtttaat tccctgccgc caaaaatccg gcgattcaat atcaaaaccg
     1141 gctctggcgg ttacctttac cgcatccatt cttcccgtat cgagcaagat atcctcatag
     1201 acaccggcaa atgaagagcc ctcctgacgg gatcggccgt acagggcaag agcaaatagc
     1261 tgtccaaagg cataggggaa attataaaac gcaagatcgc ttgaatagta atgtcccttt
     1321 accaaccaca tatatggatg aagggcctgg ccatcaaggc cgtcgccata cgttgccttt
     1381 tgattggcaa tcatcatctg acatagttgc tcgacaggaa tttctcccga agcgcgtgcg
     1441 gcaaacaca
//
LOCUS       DAOQXQ010000525         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112460_length_1448_cov_2.562096, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000525 DAOQXQ010000000
VERSION     DAOQXQ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112460_length_1448_cov_2.562096"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKH81_08230"
     CDS             <1..185
                     /locus_tag="PKH81_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98064.1"
                     /translation="DARVCMELFLVCVKKLCLERGCAVPHTIQTEAPSKDLVNHHKAE
                     EDIYEEDGSEDWIEEQ"
     gene            complement(197..1285)
                     /locus_tag="PKH81_08235"
     CDS             complement(197..1285)
                     /locus_tag="PKH81_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ98065.1"
                     /translation="MKQKTESNTKKPSKRLVKAAPLDEESLNDEDMSLDETTGEDSPV
                     VSPKLKRDTTAEFDSKENDPLALYLKQISRYPLLTITDEQEIGQKIQKIKLAVVSLNE
                     AFQDRPSDPAFLRESGKLEAQLRELKNRMITANLRLVVSIAKNYQHRGLGLLDLIDEG
                     NIGLIEAVERFDYTRGCRFSTYGTWWIRQAIIKSLADKGRIIRIPIHMLNTISKCFFV
                     AKQLTQDLGRDPSPEELSEYLQIPPNRIREIMNVSQETTSLDTTVDDDNNTRLSDLIS
                     DDSLIEPFELVFTATLQETMDDVLSQLTEREMNIIKLRFGLTDKGPLTLEETGKLLGI
                     TRERVRQIQGKAIGKLRNHEVLQALYKD"
     gene            complement(1315..>1448)
                     /locus_tag="PKH81_08240"
     CDS             complement(1315..>1448)
                     /locus_tag="PKH81_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HNQ98066.1"
                     /translation="QTIAFGEQMGTLGSDSLSGKPQLYFMVYNKDVPVDPAKAPRGY"
BASE COUNT          413 a          304 c          298 g          433 t
ORIGIN      
        1 atgatgctcg agtatgcatg gaattatttc tggtatgtgt taagaagctt tgcctggaga
       61 gaggatgcgc agtcccgcat acaatccaaa cagaagcgcc ttcaaaagat ttggtcaatc
      121 atcataaagc cgaagaagat atatacgaag aagatggatc agaagattgg attgaggaac
      181 aataaaagca gctgatttaa tccttgtaga gcgcctgtaa tacctcgtgg tttctgagtt
      241 taccgattgc cttaccctga atttgcctaa cgcgctcacg agtaataccc aacaactttc
      301 cggtttcctc aagagttaat ggccctttat ccgttaatcc aaacctgagt ttaattatat
      361 tcatttcacg ttctgttaat tgactgagta catcgtccat agtctcttgt aaagtcgccg
      421 taaaaacaag ttcaaagggt tcaattagag agtcatcgct aattaaatct gataaacgag
      481 tattgttatc atcatcaaca gtggtatcaa gacttgttgt ttcttgagaa acattcataa
      541 tctcccgaat tctgttaggt ggtatttgaa gatactcgga cagttcctct ggactcggat
      601 cgcggccaag atcctgagta agctgctttg cgacaaaaaa gcacttgctg atagtattga
      661 gcatatgaat gggaattcgg ataattcgtc ccttatcagc aaggctttta attattgcct
      721 ggcgaatcca ccaggttcca taggtagaaa atcggcatcc acgggtataa tcaaagcgtt
      781 caaccgcttc aatcaaacca atatttcctt cgtcaatcaa atcaagaaga ccaagtcctc
      841 gatgttgata atttttagca atcgaaacaa ccagacgaag attagctgtt atcatcctgt
      901 tttttaactc gcgtagttga gcttccagtt tgccggattc acggaggaaa gccggatcgg
      961 aaggacgatc ctgaaacgct tcattgagag aaaccacagc aagcttgatt ttttgaatct
     1021 tttgtccaat ctcctgttca tcggtaatcg tcaaaagagg atatcgggag atttgtttaa
     1081 gatataatgc aaggggatca ttttccttgg aatcaaactc cgcggttgta tcccgtttta
     1141 attttggaga tacaacaggg gagtcttcgc cagttgtttc atccaaagac atatcttcat
     1201 cattcagaga ttcttcatcg agaggcgcag cctttaccag gcgttttgac ggtttctttg
     1261 tgttactttc tgttttttgc ttcattcgtc cacgcctgtt acttaagata tttcttaata
     1321 cccccggggc gccttggcag gatccacggg aacatcctta ttatagacca taaaatacaa
     1381 ttgcggttta ccagaaagtg aatctgagcc cagtgtgccc atttgctcac caaaagcaat
     1441 cgtttgac
//
LOCUS       DAOQXQ010000526         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112524_length_1448_cov_1.671931, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000526 DAOQXQ010000000
VERSION     DAOQXQ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112524_length_1448_cov_1.671931"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1146
                     /locus_tag="PKH81_08245"
     CDS             142..1146
                     /locus_tag="PKH81_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNQ98067.1"
                     /translation="MAKLVSILIPLYNAEQWFRETIESALSQTWPNKEIIIVDDGSTD
                     NSLQLAKQYESKIVRIVSQENGGAAAARNKAYEYAQGDYIQWLDADDLLAPDKIENQM
                     RVAEETGPQVLLTSSWAKFYYRYKKAKFRPDNLWQDLNPVDWLITKFNENVFMNPAVW
                     LVSRELSDLAGPWNVRTAPDDDGEYIARVVVASKKVRFVPEARCYYRKSNPASLSKDP
                     SDKALQGILLSMRMSTSYLLSLEDSERTRAACIKVLQTSAGIFSRGRGDILKKADELA
                     IELGGQLSSPIVNWKYALTKRLLGPNLAKRTKMASARVKQTVAWNWDKLLYNLSEKRI
                     "
     gene            1179..>1448
                     /locus_tag="PKH81_08250"
     CDS             1179..>1448
                     /locus_tag="PKH81_08250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNQ98068.1"
                     /translation="MREEIHISAVIPAYNRGKTVARAIDSVLAQDYPASEIIVIDDGS
                     KDATGEIVRKYGDKVRYVYQDNAGVAAARNRGASEAKYEWIAFLDS"
BASE COUNT          431 a          300 c          381 g          336 t
ORIGIN      
        1 tattcagggt tctactcctc tcctggagag attcagcagg caattaacga taacgggaga
       61 gaaacgctgg ctgaagggat tgtatctcga cgtgccggaa gaatgggatg acccgtaccg
      121 ttttttccgc tggtgaaggc tatggctaaa cttgtttcta tccttatacc cttatacaac
      181 gcggaacaat ggttccgtga aaccatagag tcggccttat cccaaacatg gccaaacaag
      241 gaaataatca tcgttgatga cggatctacc gacaattcac tgcaactcgc aaaacagtat
      301 gaatcgaaga tagtaaggat agtttcccag gaaaacggag gcgctgctgc ggcgaggaac
      361 aaggcatacg agtatgcgca gggagactat attcagtggc ttgatgcaga tgatcttctc
      421 gcgccggaca aaattgagaa tcaaatgaga gtggcagaag agactggacc gcaagttttg
      481 ctaacctcat cgtgggcaaa attctactac cgatacaaga aggccaaatt tagacctgac
      541 aacctctggc aggaccttaa tcctgtagac tggcttatca caaaatttaa cgaaaacgtg
      601 tttatgaatc cagctgtatg gctcgtaagc cgtgaattat cggatctggc aggtccgtgg
      661 aatgtaagaa cagcgccgga tgacgatgga gagtatattg cacgggtggt agttgcctct
      721 aaaaaggtca ggtttgtacc ggaggcaaga tgttattaca gaaaaagcaa tccggcgagc
      781 cttagcaaag atccttcaga taaagctctt cagggtatcc ttctttcaat gcgtatgagt
      841 actagttatc tattatccct ggaagacagc gagagaacca gggcagcatg cattaaggtg
      901 ctccaaacca gtgctggaat attcagtcgg ggtcgcgggg atattttgaa aaaggcggat
      961 gaacttgcta ttgagctcgg tgggcagttg tcttctccta tcgttaactg gaagtatgct
     1021 ttaacgaaaa gattacttgg cccgaatttg gcaaaaagaa caaagatggc atcagccaga
     1081 gtaaagcaga ctgtagcatg gaactgggat aaactgttat acaatttatc agagaagcgt
     1141 atatgaagcc aatggaacca ggcggattag attgcagagt gcgtgaagaa atccatatta
     1201 gcgcagtaat tccagcctat aaccggggaa aaaccgtggc ccgggcaatc gacagcgtgc
     1261 ttgcgcaaga ttaccctgcc tctgaaataa tagtgatcga cgacgggtca aaagacgcca
     1321 ctggggaaat tgtacgaaaa tatggcgata aggtgcgtta tgtttatcag gacaatgcag
     1381 gcgttgccgc cgccaggaat cggggcgcct ctgaggcaaa gtacgaatgg atcgccttcc
     1441 tggattcg
//
LOCUS       DAOQXQ010000527         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112706_length_1446_cov_2.844716, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000527 DAOQXQ010000000
VERSION     DAOQXQ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112706_length_1446_cov_2.844716"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKH81_08255"
     CDS             <1..504
                     /locus_tag="PKH81_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98069.1"
                     /translation="NRYHQVGFDLATVLAGINLRGEFGANMTEDISGDDPSIYNPSLV
                     WSLGFDSSLFAGITFNVQVAGSVRLFDDDVGSKPFDIEDGTDITKTRITTIVSRKFLR
                     DQLEIKGTMISGIEDTDYYLIPSASWTRDDIVFELSAGFFGGSRNGELGCYRENDYLS
                     ASITLAF"
     gene            complement(659..>1446)
                     /locus_tag="PKH81_08260"
     CDS             complement(659..>1446)
                     /locus_tag="PKH81_08260"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98070.1"
                     /translation="QYPGFTHAGADGDGLDTDGSGGTFFLNSFSWSILSGTADDTQIA
                     SMLEVVRKKLKTPFGLKLVSPNDLSRVVSDAASAEYFPGDRENGAVFKHASMMAVSAM
                     LDAAKRVQDPSLAKELAGEAWTMIDLVYPGRTMADPFTLAGNPRFCTQYNNSETGENI
                     GPLLSGTSTWFILALLKAYGIELSGKGLTMDPILRPEDRSMALTLTMGDATVHIHYEK
                     DEGFTRAKDCTMSVTLDGTAIKGHCIPLPLSAGEHELTVRLTY"
BASE COUNT          352 a          334 c          356 g          404 t
ORIGIN      
        1 aatcgctatc atcaggttgg atttgatctt gcaaccgttt tggcagggat taaccttagg
       61 ggtgagttcg gtgcaaatat gactgaggat atctctggcg atgatccgtc catatataac
      121 ccatcgctgg tatggtcgct gggttttgac agttctttat tcgcgggaat tacctttaat
      181 gtgcaagtgg ccgggtccgt gcgactgttt gacgatgatg taggatcaaa accctttgat
      241 attgaggatg gcaccgatat aacaaagaca aggataacga caatagtctc gagaaagttt
      301 ttacgtgatc aacttgaaat aaagggtact atgataagcg gcattgaaga cacagattat
      361 tatctaatcc cttccgcctc atggactcgc gacgacattg tctttgagtt gtctgcggga
      421 ttctttggtg gttcacgcaa cggagaatta ggctgctatc gggagaacga ttatctttcg
      481 gcttcaatta cccttgcgtt ttaatcatac gttttatgta tggctgagta ttgactaagc
      541 gattcgaagg cttgctcctc ggtaacacag gcgaatacaa aagattccgt tgaaaggcga
      601 gggttcatct cctttaataa agtagctaga tctgaaatcc ccgccattat tttctccctt
      661 aatatgtaag cctaacagta agctcatgct ctcctgcaga aagcgggagc gggatacaat
      721 gtccctttat cgccgttccg tcaagggtaa cactcatcgt gcaatctttt gctcgagtaa
      781 agccctcgtc tttttcatag tggatatgta ctgttgcatc tcccatggtc agcgtaagtg
      841 ccattgatcg atcctcaggt ctcaggatgg gatccatagt caaacccttt ccggaaagtt
      901 caatgccata ggccttaaga agggcgagaa taaaccatgt tgaggtaccg ctcaataatg
      961 gtccgatgtt ctcccctgtt tcgctattgt tgtattgcgt acaaaagcgg gggttgccgg
     1021 caagggtaaa gggatcggcc atggttcgac cgggatatac cagatcgatc atagtccatg
     1081 cttctccggc tagctccttt gctaaagaag ggtcttgcac ccgcttcgcg gcgtcgagca
     1141 ttgcagatac cgccatcatg ctcgcgtgct taaatacagc cccgttttcc cggtcgccag
     1201 ggaagtattc ggcgcttgcc gcgtcactga ccacccggct caggtcattg ggagatacca
     1261 gctttagccc gaacggtgtt ttaagttttt tgcgtactac ctcaagcatg gaggctattt
     1321 gtgtatcgtc cgcagttccc gaaagaatag accaggagaa gctgttaagg aagaaggttc
     1381 caccgcttcc gtctgtatca agaccatccc cgtcagctcc tgcatgggta aatccgggat
     1441 actgat
//
LOCUS       DAOQXQ010000528         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_112825_length_1445_cov_3.039568, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000528 DAOQXQ010000000
VERSION     DAOQXQ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112825_length_1445_cov_3.039568"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKH81_08265"
     CDS             <1..694
                     /locus_tag="PKH81_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ98071.1"
                     /translation="IQHGADVNVCKKGNMTPFCYATSSEYIEIMRMLIKAGADIRTPY
                     YSYSSYTSPMKEALSTYGSRERNPEVVEILLDAGAGFESQIEKEDPPLLIAVRDSKPE
                     IVKLLLDKGAPLFITKGLRDSEKSILDAAAWKNRQVILDKINALFVGKTFTTQDNLRL
                     RNRSGTGAETLLTLLKGSRVHVVETGSIDTQENIQSCWVKVRTEPGAKNREGKSIKEG
                     TEGWVFAGFLEE"
     gene            complement(708..>1445)
                     /locus_tag="PKH81_08270"
     CDS             complement(708..>1445)
                     /locus_tag="PKH81_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ98072.1"
                     /translation="GFRSGAVDYVSKPFQKEEVLARVSTHISLREAKRCIEIEREKSE
                     RLLHTILPKRVAEELKNTGVSEPELFSDVSVFFSDFVNFTQNARSLSAKELIGELNAM
                     YTAFDDIMERNGCERIKTIGDAYLAVCSISGKVSDHASRMARSAREIVDFLIERNKGQ
                     ALQWKIRIGLHCGDIVGGIVGTKKYLYDIFGDTVNVASRMESLAGEMSICCTKKFCES
                     AGPGFSFCDERKIDVKGAGEMTVCTLG"
BASE COUNT          418 a          364 c          333 g          330 t
ORIGIN      
        1 tatacaacac ggcgctgatg taaatgtctg caaaaaagga aacatgacgc ctttttgtta
       61 tgccacctcg agcgagtaca tcgagattat gcgcatgctt atcaaagcgg gagcggatat
      121 tcgcacgcct tattattcct atagctcgta cacgagcccg atgaaagaag cgctcagcac
      181 ctacggatca agagaaagaa atccggaagt agtagagata ctgcttgacg caggcgcggg
      241 gtttgaaagt caaatagaaa aagaggatcc accgctactg attgccgtac gcgattcaaa
      301 gcccgagata gtaaaactcc ttcttgataa aggggctccc ctttttatta caaaggggtt
      361 acgagatagc gaaaaaagca ttctggacgc agccgcatgg aaaaacagac aggttatact
      421 cgacaaaata aatgcactgt tcgttggcaa aacctttacc acacaagata accttaggct
      481 tcgcaataga tcagggacag gggcggaaac attacttaca ttgctaaaag gctcccgcgt
      541 acatgtggta gaaaccggtt ccattgatac acaggagaac attcaatcat gctgggttaa
      601 agtacgcaca gaacccggcg caaaaaatag agaaggtaaa agcattaaag aaggaactga
      661 aggctgggtc tttgcaggct ttttagaaga gtaaaaaaga cccctcctta gccaagagtg
      721 cacaccgtca tctcgccggc gccttttaca tcaatcttac gttcgtcgca aaaagaaaac
      781 ccgggaccgg ctgactcgca gaattttttc gtacagcaga tactcatctc gccggcgagg
      841 gattccatgc gcgaagcaac gtttaccgta tcaccaaaga tatcgtacag gtatttttta
      901 gtgccaacga tgccgccgac gatatccccg caatggagtc ctatcctgat cttccattgc
      961 agagcttggc ccttgttccg ctcaattaag aaatcgacga tctcgcgcgc cgagcgggcc
     1021 atgcgcgacg catgatcgct cactttgccg gaaattgaac acaccgcaag gtaggcatcc
     1081 ccgatagtct tgatcctctc gcagccgttc ctctccataa tatcgtcaaa ggcggtatac
     1141 atcgcattaa gctccccgat cagctccttg gccgaaaggc tccgcgcgtt ttgtgtaaag
     1201 ttgacaaagt cggaaaagaa aaccgacacg tcgctaaaga gctcgggctc gcttactccg
     1261 gtgtttttta attcctcggc aacccgttta ggcagtatgg tatgaagaag tcgctcagat
     1321 ttttcccgtt caatttcgat acagcgcttt gcctctctaa gtgaaatatg cgtagaaacg
     1381 cgggctaaaa cctcttcctt ttgaaacggc tttgacacat aatccaccgc acccgagcgg
     1441 aaccc
//
LOCUS       DAOQXQ010000529         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_113082_length_1443_cov_3.514409, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000529 DAOQXQ010000000
VERSION     DAOQXQ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113082_length_1443_cov_3.514409"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKH81_08275"
     CDS             <1..1085
                     /locus_tag="PKH81_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNQ98073.1"
                     /translation="SVEPAEAEEALASCNARILTLDAEMKQYEEELSRLRSERSTLIG
                     KRDTQQKSITAHLAQLSESEKEFLLARQAALFPTEDAVSQAYLEEAECSHLEEQNAQW
                     RRDRDACKSAIERLDKEVGSWQGPASHTIEEECTRLAEQSKSLSEQLEEKNAERVSLT
                     SLCEQFAALEAQRAERSLKAGRLKALSDDLSGNNSRKISFDAWILGMYLEEITEYANL
                     RLQRISDGRYRIQLNDSYRKGNSLAGLDLEILDSYTGKTRPTGTLSGGETFITSISLA
                     LGLADSIQSRSGGIELDAVFIDEGFGSLDEASLERAISILDEIRGTRMVGIISHVVDL
                     RNRIPNRIEVVKTPSGSRIEKESCNE"
     gene            1078..>1443
                     /locus_tag="PKH81_08280"
     CDS             1078..>1443
                     /locus_tag="PKH81_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNQ98074.1"
                     /translation="MNNSALFTDFYELTMAQGYWKRQSNHKAVFDMFFRRQPFSGGFS
                     VFSGLDPLLDAIESFRFSPEDIAFLDSQKKFEQGFLDYLSNFRFTGEIHAMKEGTIVF
                     PNEPLLRIHANIIEAEIIEG"
BASE COUNT          406 a          357 c          343 g          337 t
ORIGIN      
        1 ccagcgtaga accggcagag gcagaagaag cccttgcttc atgtaatgcc cgcatcctta
       61 ccctggatgc cgaaatgaag cagtacgaag aagagctgtc tcgactgcgc agcgaacgtt
      121 caacccttat tggtaaaagg gatacccaac aaaagtcgat aaccgcacat cttgcccaac
      181 tgagtgaaag cgaaaaagag ttccttcttg cccggcaggc agctctcttt cccacagagg
      241 atgcggtatc ccaggcatat cttgaggaag cggagtgtag ccaccttgaa gagcaaaacg
      301 ctcaatggag gcgcgaccgt gatgcctgta agtcagcgat cgaacggcta gacaaagaag
      361 ttggatcgtg gcaggggccg gcctcgcaca ccatcgaaga agaatgtacg cgtcttgctg
      421 aacagagtaa gagcttgagt gaacaactcg aagaaaaaaa cgcagagcgc gtttccctta
      481 cctctctttg cgagcagttt gccgctctgg aagcgcaacg ggcggaacga tcactcaagg
      541 caggtcggct taaggccttg tctgatgact tgagcggaaa caatagccgg aaaataagct
      601 ttgacgcatg gatactggga atgtatcttg aagaaataac tgaatacgca aaccttcgtc
      661 tccaacgcat cagcgacgga cgataccgca ttcaactgaa cgacagttac cgcaagggaa
      721 acagccttgc cggccttgat ttggaaattc tggattcata tacgggaaaa acaaggccca
      781 ccggaacact ctccggagga gaaaccttta tcacctccat aagccttgcc ctcggccttg
      841 ccgactcaat ccagtcacgc tccggcggca tagaactgga tgcggtcttt attgacgagg
      901 gatttggatc tcttgatgag gcaagccttg aacgtgcaat ttctatcctt gacgaaattc
      961 gcgggacccg tatggtcggc attatctctc atgtggtaga cttacgaaat agaataccaa
     1021 acagaatcga agtagttaaa actccttccg gttcacgaat agaaaaggaa tcttgcaatg
     1081 aataacagcg cgctttttac cgatttttat gaattaacca tggctcaggg ctattggaaa
     1141 cgccagtcaa atcataaagc cgtttttgac atgtttttcc gccgtcaacc cttctcgggc
     1201 ggtttctccg tattttccgg acttgatccc cttcttgatg cgattgaatc cttcaggttt
     1261 tctcctgaag atattgcgtt tcttgacagt caaaaaaaat ttgaacaagg ttttcttgac
     1321 tacctttcaa actttcgttt taccggagaa atccacgcga tgaaggaagg caccatcgtt
     1381 tttccaaacg agccacttct acgcatacac gcaaacatca ttgaagccga gattatcgaa
     1441 ggg
//
LOCUS       DAOQXQ010000530         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_113723_length_1439_cov_1.455925, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000530 DAOQXQ010000000
VERSION     DAOQXQ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113723_length_1439_cov_1.455925"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..363
                     /locus_tag="PKH81_08285"
     CDS             238..363
                     /locus_tag="PKH81_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98075.1"
                     /translation="MPILAAFIGLVSIFIGIADLKDKQEARKEEEAAKAAAAKDN"
     gene            491..919
                     /gene="rplM"
                     /locus_tag="PKH81_08290"
     CDS             491..919
                     /gene="rplM"
                     /locus_tag="PKH81_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ98076.1"
                     /translation="MKTLFVKEHEAPRSWFVIDAQDKPIGRIAAKAASMLRGKHKVTY
                     APNQETGDYIVIINAEKAVLSGTKTEDKMYRHHTGFVGGLRSVSYAKLLERHPSDPIL
                     LAIKGMLPKGPLGRKMLTNVKVYAGTDHPHAAQNPQAVEV"
     gene            937..1325
                     /gene="rpsI"
                     /locus_tag="PKH81_08295"
                     /pseudo
     CDS             937..1325
                     /gene="rpsI"
                     /locus_tag="PKH81_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
BASE COUNT          352 a          335 c          378 g          374 t
ORIGIN      
        1 tagacggagg tagttgatcg cagtgcatgc cttactatat caagagggga ttctgttgac
       61 catcccccgg aagtgtgaga aaatcgatag tgcttactgt caggaggcct tgattatggt
      121 agctttagtt gttggaataa ttcttattgc gtttaccgtt tttgcggctt cgccccggcg
      181 ctgttgggtt ttgggctcgg ttggggtgcg gatattctgc tgtttctccg tggttgtatg
      241 ccgatccttg cagcgtttat cggtttggta tccattttta ttggaatcgc cgaccttaag
      301 gacaaacagg aagcccgtaa agaagaagaa gcggcaaaag ctgctgctgc caaggataac
      361 tgatctcgcc ctccgctatg ggcggtcttg acattcatcc tgctttttct ctatagtaaa
      421 caacccacat tatctgcgtt tgaccgcaac gaatcgcggt caacagacta ctaatttgta
      481 aggtatgcac atgaagacac tttttgttaa agaacatgag gcaccgcgct cttggtttgt
      541 tattgatgca caggacaaac cgattggacg cattgccgcc aaggccgcca gcatgttacg
      601 gggcaagcat aaggtaacct atgcacccaa ccaggaaact ggtgattata tcgtcattat
      661 caatgccgag aaggcggttc tttccggaac caagaccgag gacaagatgt atcgtcatca
      721 taccggtttt gttggtggac ttcgctcggt tagctatgca aagctcctgg agcgccatcc
      781 gtcggatccc atccttcttg ctattaaggg aatgcttccc aagggaccgc tgggtcgcaa
      841 gatgctgacc aatgtcaagg tatatgccgg tacggatcat ccccatgccg ctcagaaccc
      901 ccaagcggtt gaagtataac gaggagtaat caggtgatca aaaatattgg aatgggaaca
      961 ggaagaagga agacagcggt agcccgtgtg tttgtccgcg aaggaaaagg aaacctcctg
     1021 gtaaatgata aggacgtttc tgattacttt gctaccccgg agcaggttca ggttgttcgc
     1081 caaccgctta tggtaaccgc cagcgatacc aaattcgaca tcgttattac tgttaacggc
     1141 ggcggattaa acggacaggc cggtgcatgc agtcatggta tcgcccgagc tctttcccag
     1201 gtagacgtag agaatcatgc ctctcttaag gcaaacggtc ttttacccgc gattctcgta
     1261 tggttgagcg caagaagtat ggtcagcgtg gcgcacgcag aagattccag ttcagcaaac
     1321 gctaatacag gaatttccat ccttcatata gaacgggcct cagccggtgt acttacagta
     1381 cagccggtcg aaggcccttc ttttttggtt cgggaggtat atgtggcaga gcctcttcg
//
LOCUS       DAOQXQ010000531         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_114555_length_1432_cov_3.572985, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000531 DAOQXQ010000000
VERSION     DAOQXQ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114555_length_1432_cov_3.572985"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKH81_08300"
     CDS             <1..682
                     /locus_tag="PKH81_08300"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNQ98077.1"
                     /translation="LSIIAVALPIACNLLYVFRLIPGIKRDFSSLVFAISGLLFMISI
                     IRYRLFDTLPIEYKNVMDTISKSLILVDATGIIQDINEQALSIPGFSALIGKHISDSL
                     GIQMTVVEDAALSNGATSFSLILSGEHEYICFAERIKFNSEDSGFSITLTQASPASLS
                     FRMSRREQAVYTFLVEGLTTKEISEKLCISENTTKTHIRHIFEKLQVNNRKDLKGLTG
                     QAPQSGRA"
     gene            complement(679..>1432)
                     /locus_tag="PKH81_08305"
     CDS             complement(679..>1432)
                     /locus_tag="PKH81_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ98078.1"
                     /translation="TSGRESSLIIEPLYYREERLGYIVFEQTSRSARVYESLAAQISS
                     ALEGAVLIERVMSAEKAIQERSRQIEALVKPLMDSIRSVAQTTSEQHVVISKLEDLNQ
                     KSLKSVNGMEGNTRALAEAIEKTGALVGEINNISEVINVVAINASIEAAHVGREGAAF
                     AVIAGEVRKLSASTRQNSERITGFLGEVETKISSLAQFNKELSSAFAELNATIRATVS
                     TLETITGRMEGLDRGSGEILSLMNDRGQTSHY"
BASE COUNT          333 a          361 c          315 g          423 t
ORIGIN      
        1 tctttcaatt attgcggtgg ccttgccaat cgcttgtaat cttttgtatg tgtttcgtct
       61 gattccggga atcaaacgcg atttttcttc gcttgtgttt gccattagtg gtttgctttt
      121 tatgatatcg attattcgct atcggctttt tgacacgctt cctattgagt ataaaaacgt
      181 aatggatacc atcagcaagt cgctgatcct cgttgatgct accgggatta ttcaggatat
      241 caacgagcaa gctctgagta ttcccggctt ttccgctctt atcggcaaac atattagcga
      301 ttcactcggt atccagatga cagttgtgga agacgcagct ctttcaaacg gtgcgacctc
      361 tttttcactg attctttcgg gtgaacatga atacatttgt tttgcggaac gtattaaatt
      421 taatagcgag gattcagggt tctccattac ccttacgcaa gcttctcctg cttctttaag
      481 ctttcgcatg agtcgacggg aacaagccgt atataccttc ttggttgaag ggcttaccac
      541 caaggagata tcagagaagc tgtgcatttc ggaaaatact accaaaaccc atatcagaca
      601 catcttcgag aagctgcaag tcaataaccg caaggacctt aagggcttaa cggggcaagc
      661 cccgcaaagc ggtcgcgctt aatagtgact cgtctgtcct ctgtcattca tcaaggacaa
      721 tatttcacca ctgccacgat cgagcccttc catgcgacct gtgatggtct caagggtact
      781 tactgttgcc cggattgtcg cgtttaactc tgcgaaggcg gaggacagtt ccttgttgaa
      841 ctgggcaagc gaggatatct ttgtctcaac ctcgccaagg aatccggtta tccgctcaga
      901 gttttgccgt gttgaggcag agagttttct tacttcgccc gcgattacgg cgaaggcagc
      961 accttcgcgt cctacatggg ctgcctctat actcgcgttt atggcaacga cgttgataac
     1021 ctcggagata ttatttatct cgccaacaag cgcacccgtt ttttctatgg cttctgccaa
     1081 agcgcgagtg tttccttcca tcccgttaac agacttcagg ctcttttgat tcaggtcttc
     1141 caacttggaa ataaccacat gctgttcact cgttgtttga gcaacagagc ggatcgaatc
     1201 catcagtggt tttaccagag cctctatctg tctactgcgc tcctgtatcg ctttttctgc
     1261 ggacatcact cgctctatca acaccgcccc ttcgagcgca ctactgattt gagctgccag
     1321 agattcatag acacgagcag accgggatgt ttgctcaaag acgatatatc caagtcgctc
     1381 ttcccgataa tacaagggtt caataatgag tgagctctcc cgtcccgaag ta
//
LOCUS       DAOQXQ010000532         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_115292_length_1427_cov_2.701895, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000532 DAOQXQ010000000
VERSION     DAOQXQ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115292_length_1427_cov_2.701895"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKH81_08310"
     CDS             complement(<1..606)
                     /locus_tag="PKH81_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNQ98079.1"
                     /translation="MNTSGKGMSVRALSIIGITCFIVCVLLIGCTRDSDTRLAVDKDG
                     KTVVERYGRLQVIGTNLCNEAGQPVQLRGMSSHALHWYGKYANAMVLKWLRDDWNAQL
                     WRAAMYVTEGGYMTGKAVKFKMIDSIEAAIENGMYVLVDWHVHRDRNPNIYKKEAIEF
                     FTEIAQQYHSYPNIIYEICNEPNGDDVSWSGEIKPYAEDVIA"
     gene            complement(676..>1427)
                     /locus_tag="PKH81_08315"
     CDS             complement(676..>1427)
                     /locus_tag="PKH81_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ98080.1"
                     /translation="LHFFLEGSLSFQCNRTKYPVSPGRLFLTGPYDFHSILPEAVSTP
                     VTYYAVLFSLDSAGSSVERSLRQSLYEAIAGRAVILSIDAHFRFQFEDLLLMSKSADP
                     ALNESALHLLQSFLYRWFCRSSSLQGEARSEERKTNRLHAQKALALMEKQVRENILVE
                     DFARKLGLSDEHFIRVFRDEVRMTPHQYFTRLKVEGASGMLMSTDKSVGEISDWFGFE
                     NQFHFSRIFKKCTGMSPLQYRRTYLQIVDFS"
BASE COUNT          426 a          346 c          289 g          366 t
ORIGIN      
        1 agcgataacg tcttccgcgt agggtttgat ctctccgctc caagacacat catcgccatt
       61 gggctcattg cagatttcgt aaataatatt gggatatgaa tgatactgct gggcaatctc
      121 ggtaaaaaac tctatggctt cctttttata gatgttggga ttacgatccc tatgcacgtg
      181 ccaatccact aacacataca tgccattttc aattgccgcc tctatagaat cgatcatttt
      241 aaacttaaca gcctttccgg tcatgtagcc accttcggta acatacatgg ctgctcgcca
      301 caattgcgcg ttccaatcgt cccgcaacca cttcaatacc atggcattgg catacttacc
      361 gtaccaatga agggcatgag aactcatacc cctaagctga acgggctgcc ccgcttcatt
      421 acatagattt gtcccgataa cctgaagccg tccatagcgc tcaacaaccg tctttccatc
      481 cttgtcgacg gctaaacgag tgtcgctatc gcgcgtacac cctatcaaca agacacacac
      541 aataaagcaa gttattccta taatagacaa agcgcgtacg ctcatacctt tacctgatgt
      601 attcataggt ccatcctcct gtgcaaaagt atatcatgac attagtatgt tgcgatagta
      661 aacaaataca gtgttttacg agaaatcaac gatttgaaga taggttcttc tgtattgaag
      721 aggagacatc ccggtacatt ttttgaaaat ccgggaaaaa tgaaattgat tttcaaaccc
      781 gaaccagtcg gatatctctc caaccgattt gtctgttgac atgagcatac cgctggcacc
      841 ttctaccttt agccgtgtaa agtactggtg aggcgtcata cgaacctcgt cgcgaaacac
      901 tcttataaaa tgctcgtcgg acaaaccaag cttgcgagca aaatcttcca ccaaaatgtt
      961 ctcccgaacc tgcttctcca taagagccag agctttttgg gcatgcaaac gatttgtttt
     1021 acgctcctct gaacgtgctt cgccctgcag ggaacttgac ctgcaaaacc atcgatataa
     1081 aaaactttgc aacaggtgta gagcagactc gttaagggca gggtcggcag attttgacat
     1141 taatagaaga tcttcaaact gaaacctgaa atgtgcgtca attgacagta ttaccgcacg
     1201 acctgcaata gcttcataca gggattgccg aagagagcgt tcaacagaag accccgctga
     1261 atcaagtgaa aaaaggactg cgtaataggt gactggagtt gaaacagcct ctggaagaat
     1321 cgaatgaaag tcatatggac cggttaaaaa aaggcgccca ggcgacacag gatatttagt
     1381 tcggttacac tgaaaagata gcgatccttc caagaaaaag tgtaatt
//
LOCUS       DAOQXQ010000533         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_115568_length_1425_cov_2.943066, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000533 DAOQXQ010000000
VERSION     DAOQXQ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115568_length_1425_cov_2.943066"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKH81_08320"
     CDS             complement(<1..421)
                     /locus_tag="PKH81_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNQ98081.1"
                     /translation="MKMSRMFMPTLREVPAEAVIASHRLLLRSGMIRKLGNGLFTYLP
                     LGLRSFRKVEAIIREEMNASGALEFKPSVVVPGEIWRESGRWETMGAGMLRVVNRVDQ
                     ELVVSPTAEEAFTALLRSEISSYKQLPINVYQINTKYR"
     gene            543..>1425
                     /locus_tag="PKH81_08325"
     CDS             543..>1425
                     /locus_tag="PKH81_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ98082.1"
                     /translation="MRKTIAVLTNHMGGAKLPYERSFVSAIADAAKQNDDKVIFYSGG
                     FYNTPDRFESQRNFVYELVERDRPDGIIMLSSLLGEFCPKKDIYALCKTFGSIPLVSS
                     GMSVPGVPSVLINNYVAMRSVTDHVFSVNTPGKIVYLGGPKDGDEAQERFRAFSDSLT
                     NAGYRLEDVRIFEGIYLKISGCNAALKLLEEDTPFDTVISASDEMAFGFVECMEEHGI
                     RCPDDFRITGFDNVKGASLFSPALTTVEQNTYEMGTASFEALRRIINGEPAQEVTWLP
                     TRLLIRDSCGCGGGALPE"
BASE COUNT          331 a          371 c          372 g          351 t
ORIGIN      
        1 cgcgatattt ggtgttaatc tgatagacgt tgatgggtaa ctgcttatac gaggatattt
       61 cacttcgcag gagtgcggta aaggcttctt ctgcggttgg actcaccacc agttcctgat
      121 ccacccggtt taccacacgg agcattcctg cgcccatggt ttcccaccga ccggactccc
      181 gccaaatctc gccgggaacc actaccgaag gtttaaactc aagggcaccg gatgcattca
      241 tttcctcgcg gataatcgct tcaaccttac ggaacgaacg caacccgagc ggtaaatacg
      301 taaacaagcc attgccgagc ttgcggatca ttccggagcg gagcaacagg cgatgactgg
      361 cgataacagc ttcggcgggt acttcccgca gtgtcggcat gaacatcctg gacattttca
      421 tgatacaaaa ccccttgtgt gctggcattt tagccgtaat cggcgccttt atcaaggaga
      481 gaaagacgcg cgcgcgccct gcttttgttt gacggcagga cagggctctg gtactataag
      541 gtatgcggaa gaccatcgct gttttgacca atcacatggg cggggccaag cttccgtatg
      601 aacggagctt tgtatctgcc atcgcggatg cggcaaaaca aaatgacgac aaggttatct
      661 tttactcagg cgggttttat aacactcccg atcgctttga aagtcagcga aactttgtgt
      721 atgaattggt agagcgggac cgtcccgacg gtattatcat gttatcttcc cttctggggg
      781 agttttgccc aaaaaaagat atctacgccc tgtgcaaaac ctttggctcc atccccctgg
      841 taagttcagg catgagcgtt cccggtgttc ccagcgtcct gatcaacaac tacgtcgcca
      901 tgcgctcggt aaccgatcat gtctttagcg ttaatactcc cggcaagatt gtctacctgg
      961 gcgggccaaa agacggggat gaggcacagg agcgctttag ggcttttagc gactccctta
     1021 ccaacgccgg ttaccgtctg gaagatgttc gtatattcga gggtatctac ctcaagattt
     1081 ccggttgtaa tgcagcctta aagctcctgg aagaagacac tccctttgat accgttatct
     1141 ctgccagcga tgaaatggcc tttggctttg ttgaatgtat ggaagagcat ggcatccgct
     1201 gtccggacga cttccgtatt accggatttg acaacgttaa aggagccagc ttgttctccc
     1261 ctgcattaac caccgtggaa caaaatacct acgagatggg cactgcctcc tttgaggctc
     1321 tacggcggat aattaatggg gagccggcgc aagaggttac atggcttcca accaggctct
     1381 tgattcggga cagctgtggc tgcggtggag gtgccctgcc ggaga
//
LOCUS       DAOQXQ010000534         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_115847_length_1423_cov_2.726608, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000534 DAOQXQ010000000
VERSION     DAOQXQ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115847_length_1423_cov_2.726608"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="istA"
                     /locus_tag="PKH81_08330"
     CDS             complement(<1..1239)
                     /gene="istA"
                     /locus_tag="PKH81_08330"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNQ98083.1"
                     /translation="MDTKQEILRRYFRECDSERKIARDLQLNRKTVKKYLEEYLETKK
                     KSEEECNKEILQDYSTSPPDYNASGRVKRKLTLEIAEIIREQVEENERKRRNGLRKQI
                     KRKIDIHEYLLSLGYQIGYTTVCNHIRNQDVSDREAYIRQVYIPGEECEFDWAETKLT
                     IGGVQRRYFLAVFTSSYGNYRFARLYSRQDTLSFMESHNDFFAHVGGVFHEMVYDNMR
                     VAISEFAGRHEKQPTTALSNLAGWFQFRWRFCNVRRGNEKGHVERSVELVRRKAFSNR
                     DSFESLEQAQDHLMSTLDKLNRLSSCSTGKSPLMALTEERSHLWKYPGEMECYLMESL
                     KVDKYATFSYGTNRYSVPDYLVGRMVDVKVYSNRLKVYHNNLHLCSHERNYGTFQWKI
                     EIDHYLVTLSRKPGAVHGSVA"
BASE COUNT          360 a          348 c          280 g          435 t
ORIGIN      
        1 ggctactgag ccgtgaacag cgccaggctt gcgtgaaaga gttaccaggt aatgatctat
       61 ctctatcttc cactggaaag tcccgtagtt cctctcgtgg ctgcataagt gcaggttgtt
      121 gtggtatact ttaaggcggt ttgagtaaac tttgacatcg accattctcc caaccagata
      181 atcaggtaca gagtaacggt tggtcccata actgaaagtg gcatatttat cgactttaag
      241 actctccatc aggtagcact ccatctcacc cgggtacttc cacagatgcg atctttcttc
      301 tgttaaagcc atcaaaggac ttttccctgt ggaacagctg gaaagtctgt tcagcttatc
      361 aagggtgctc ataaggtgat cctgtgcctg ttcaaggctc tcaaaactat cccggttact
      421 gaaggcctta cggcggacaa gctcaacact gcgctctaca tgacccttct cgttgcccct
      481 tctgacgtta cagaacctcc aacggaactg gaaccatcct gcaaggttac taagagctgt
      541 tgtgggttgc ttttcatggc gtcccgcaaa ttcggatatc gctacccgca tgttatcata
      601 aaccatctca tggaacacac cccccacatg tgcaaaaaaa tcattatgcg attccatgaa
      661 tgacaacgta tcctgcctcg aataaagcct cgcaaaacgg taattaccat acgatgaagt
      721 gaatacagcc agaaaatatc ttcgctgtac ccctccgatt gtcaatttag tctctgccca
      781 gtcaaactca cactcttcac cgggaatata tacctggcgg atataggctt cacgatccga
      841 cacatcctgg ttgcggatat gattgcacac tgttgtatat ccaatctgat atcccaggga
      901 aagaagatat tcatgtatat cgatcttgcg ttttatctgc ttgcgcaatc cgttgcgccg
      961 tttacgctca ttctcttcta cctgttcacg gattatttcc gctatttcaa gggtaagctt
     1021 ccgttttacc ctcccggatg cattatagtc gggaggagag gtactgtagt cctgaaggat
     1081 ttctttatta cattcttctt ccgacttttt cttcgtttca aggtattctt ccaggtattt
     1141 cttaacagtc ttgcggttaa gctgaagatc cctggctatt ttcctttcgc tgtcacattc
     1201 gcgaaaatac cttctcaaaa tttcttgttt cgtatccatc tttatcatct gctttacgtt
     1261 gcgtttgact tctcaaggca acgggttaaa catttaaagt ggtatacttt tcaacgagaa
     1321 tgtggtatac ttttcaatta atatatacat aactttcccc tccttggaaa ggaggggaaa
     1381 gttatggtgc tatatatctg cacattaaac accaatttga gat
//
LOCUS       DAOQXQ010000535         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_115877_length_1423_cov_2.416667, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000535 DAOQXQ010000000
VERSION     DAOQXQ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115877_length_1423_cov_2.416667"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKH81_08335"
     CDS             <1..555
                     /locus_tag="PKH81_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNQ98084.1"
                     /translation="TTLLKDDDPAKAGFILEQLGNQEDYADQMQEELSQYLVKTSQLP
                     LSDRTRENVRLMLRIVDDLESMTDDIFSISMLIQRSRDKKMNFRGDDIELLTPYIGLV
                     ERFLNFVYENLNKPLSKDKLDFAHEMEDQIDLFRKNLKKTARKRLEEGANVKAELLYI
                     DIVRGIEKLGDHAFSISEALAATK"
     gene            complement(529..1176)
                     /locus_tag="PKH81_08340"
     CDS             complement(529..1176)
                     /locus_tag="PKH81_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ98085.1"
                     /translation="MKISKLTGGPLAVNTWFVFNTDGSAMVIDPGVSADSIIAHLKQN
                     AIEALSIVLTHGHLDHITAIAELTSQYPSAKVYCHRDDAFFLGEGAVERHRSFFEQIG
                     AGFVSRFIPEHIPQADEFLEEGQTLGEFTILHTPGHSPGSVCLYAPSEQVLFSGDTLF
                     NAGWGRTDSPGGSDAALFESLTRLSKLPGQTRVLCGHGEDTTIAREFLLRGGQSL"
     gene            1210..>1423
                     /locus_tag="PKH81_08345"
     CDS             1210..>1423
                     /locus_tag="PKH81_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98086.1"
                     /translation="MTVLVSLIILTALLFYVALPLLSREAQKKHYNGILTDIDERVGI
                     SGSVTTVRDGKIWITADHRQEDSDTPL"
BASE COUNT          371 a          369 c          357 g          326 t
ORIGIN      
        1 actaccctcc taaaagacga tgatccggcc aaggcagggt tcatcctgga acaactgggt
       61 aatcaggagg actatgcaga tcagatgcag gaagagctct cccagtacct ggtcaagacc
      121 tcgcagctcc ccctgtccga ccgtacccgc gagaacgtgc ggcttatgct tcgtattgtg
      181 gacgatcttg aaagcatgac tgatgacatc ttctccattt ccatgctgat tcagcgaagc
      241 cgcgacaaaa agatgaactt taggggagac gatattgaac ttctgactcc ctacattggt
      301 ctggttgaac gcttcctcaa ttttgtctac gaaaatctta ataagcccct tagtaaggat
      361 aagcttgatt ttgcccacga gatggaagat caaatagatt tgttccggaa gaacttgaaa
      421 aaaaccgccc gaaagcggct tgaggaagga gcgaatgtta aggccgagct actctatatc
      481 gatattgtcc gcggtatcga aaagcttggc gatcacgcct ttagtatctc agaggctctg
      541 gccgccacga agtaaaaact cccgggcgat ggtggtatcc tccccgtgtc cacacaggac
      601 gcgggtttga ccaggcagct tcgatagccg ggtaaggctt tcaaacaggg ctgcatcgct
      661 tccaccaggg ctatccgtac gaccccatcc tgcattaaac agggtgtctc ccgaaaacag
      721 tacctgctca gatggggcat agaggcaaac cgagccgggg ctatgaccgg gggtatgtaa
      781 aatagtgaac tctccgagcg tctgtccctc ttccaaaaat tcatccgctt gcgggatatg
      841 ctccggaata aaccgggaaa caaaacccgc tccaatctgt tcaaaaaagg agcgatggcg
      901 ctctactgcc ccctctccca aaaaaaacgc gtcatcccga tggcagtaga ccttagccga
      961 gggatactgg gaggtaagtt ccgcaattgc ggttatatga tcgagatgcc catgggtcaa
     1021 gacgatggac agggcctcga tggcgttttg tttaaggtgt gctataatcg agtcggcgct
     1081 tacccccggg tctatcacca tagccgatcc atccgtatta aagacgaacc aggtattaac
     1141 cgccagcgga ccgccagtaa gttttgatat cttcataagt agggcgagta taccgcgaaa
     1201 ggaggattca tgacagtcct tgtttccctg atcatcctga ccgctctatt attttatgtg
     1261 gccctacccc tgctttcgcg cgaggcgcag aaaaagcact acaacgggat tctgaccgac
     1321 attgacgagc gtgtcggtat ttccggcagt gttaccactg tccgggatgg aaaaatatgg
     1381 attacagccg atcaccgtca agaagattct gataccccgc ttg
//
LOCUS       DAOQXQ010000536         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_116918_length_1415_cov_2.600735, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000536 DAOQXQ010000000
VERSION     DAOQXQ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116918_length_1415_cov_2.600735"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..779)
                     /locus_tag="PKH81_08350"
     CDS             complement(150..779)
                     /locus_tag="PKH81_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HNQ98087.1"
                     /translation="MTSDWRDLSLIADLTEAEADLFKSSCQTLLSKSFIMRGTETSNI
                     LWDFAIRNITLLEVWFSCAGITLKRDELLGVIAMRPTPAMRAHLGKEETFALLVLRLL
                     FEEKRTELTLSAFPSIRVFDLLQRYKAVVGIDLKKTRLVEILRSFSRWKIIDAQGDLD
                     DSETTIALLPSIAMTLDQESIDEILNAIGTTETHTERDEEVIEEEGEAE"
     gene            complement(776..>1415)
                     /locus_tag="PKH81_08355"
     CDS             complement(776..>1415)
                     /locus_tag="PKH81_08355"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HNQ98088.1"
                     /translation="TSSLPVSESRERLLVMLEEIKDILKAVDPLLEEIDRRNMLYAKS
                     SVERVKVLLESESTISGKIVRIITSLYETPYVYQTLSHHLYRVRAITPESRYRRWLRE
                     TINLEYVESPTIDPAEFDRMEAEFTLRLARQLTPAKIERWLDEKGGKEKILSAKSLAD
                     TSEGFVRLLYAVLFADSQPETFAYQVEESQDAGEVTTASGWIIPEISLRRNT"
BASE COUNT          336 a          320 c          350 g          409 t
ORIGIN      
        1 aagatcagct gccagtgcgt actggatagc gtcgacgatt gttgacttgc ccattgcgtt
       61 attaccggag ataagggttc tttctcctac cgcgatggtt tcgtccctga agtagtgcca
      121 attcacgatt cttactttcc gcaaggcgat cattcagcct ctccttcttc ttcaatgact
      181 tcttcgtctc tttccgtatg tgtttcggtt gtacctatcg cattcagaat ttcatctatc
      241 gattcttgat caagggtcat cgcaatggat ggtaatagtg caatcgtggt ttcgctgtca
      301 tcaaggtctc cctgagcatc gattattttc caacgagaga atgacctgag tatttctacc
      361 agacgagttt ttttcaagtc gataccaaca actgccttat agcgctggag aaggtcaaag
      421 acgcgtattg aaggaaatgc agaaagtgtg agttctgttc ttttttcctc gaaaaggaga
      481 cggaggacaa gaagcgcgaa tgtttcttct ttaccaagat gtgcgcgcat cgcaggtgtg
      541 ggtcgcatgg cgataacccc aagaagctcg tcgcgtttga gcgttattcc tgcacaggaa
      601 aaccatactt caagcaaagt gatattccgt attgcaaaat cccaaaggat atttgatgtt
      661 tcagtaccgc gcatgataaa actcttggaa agcagggttt gacaggagct tttaaaaaga
      721 tcagcttctg cctcggtaag atcggcgata agggaaagat cgcgccagtc gcttgtcatg
      781 tattcctccg taggctaatt tctggaatta tccagccgga agcggttgtt acttcgcctg
      841 cgtcttgaga ctcttccacc tggtatgcaa aggtttcggg ttgactgtcc gcaaagagga
      901 cggcatataa gagtcgaacg aatccctcgc tagtatcagc gagcgacttg gcggagagta
      961 tcttttcttt accccccttt tcatcaagcc accgctctat ctttgccggt gtaagttgcc
     1021 gtgcaaggcg aagggtaaac tcggcctcca tgcgatcgaa ttcggccggg tctattgttg
     1081 ggctttcaac atactcaagg ttaatcgttt ctcgcagcca ccggcgatag cgtgattcgg
     1141 gtgttattgc gcgtacacgg tagaggtggt gcgaaagcgt ttggtataca tagggtgttt
     1201 cgtaaagtga tgtaatgatg cgaacaattt tgcccgatat cgtcgattcg ctttccaaaa
     1261 gaactttcac ccgctcaacg cttgatttgg cgtagagcat gtttcttcgg tcaatttctt
     1321 caagcagggg gtcaacagcc ttgaggatat ccttgatttc ttccagcatt actagtagcc
     1381 gttcgcggga ttcggaaaca ggaagtgatg aagtg
//
LOCUS       DAOQXQ010000537         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_117834_length_1408_cov_3.563193, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000537 DAOQXQ010000000
VERSION     DAOQXQ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117834_length_1408_cov_3.563193"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKH81_08360"
     CDS             <1..652
                     /locus_tag="PKH81_08360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519705.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ98089.1"
                     /translation="LREISTTRGEVSGRKGYPGYLYSDLAELYERAGKIKGSSGSITQ
                     IPILSMPNDDISHPIPDLTGYITEGQIVLDRELSQKGIYPPVAGLPSLSRLMKDGIGA
                     GMTRDDHKDVSSQLFASYSKVKSIRNLAAIIGEEELSDSDKLYLRFGELFEQKFLTQG
                     EFENRSIEETLDIGWCLLSVLPRSELYRIKAEYIETYLQAALDKDPSFLSGVKARI"
     gene            656..1285
                     /locus_tag="PKH81_08365"
     CDS             656..1285
                     /locus_tag="PKH81_08365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNQ98090.1"
                     /translation="MARLPVAPTKSNLLAIKEQLAIARDGYELLEQKREILVMELMQM
                     VEKVKLLEADIEKQIDKAYPSLKRMFMAVGRDRTERVSRTVSYDFHLVEKRVVMAGMN
                     FSTLSVELPKRRLAWSYLNSFADCDKVMIDFFELLRLLTDMASIRTIVWRLAREVKKT
                     QRRVNALDKQVIPQTRETSSYIESVLEERERENVFVLKALKAKNGGLSR"
BASE COUNT          378 a          320 c          344 g          366 t
ORIGIN      
        1 cctgcgcgaa atatcaacaa ctcgaggcga ggtttccggg cgcaagggat atcctggata
       61 cctgtattcc gatcttgccg agctctatga gcgtgcaggc aagataaagg gttcttccgg
      121 ctcaattaca cagataccaa ttttatccat gccgaacgac gatatcagtc atcccattcc
      181 ggacttaacc ggatatatta ccgagggtca aattgttctt gaccgtgagc tttctcaaaa
      241 aggtatatat cctccggtcg cgggcttacc gagtctatcc cgtcttatga aggacgggat
      301 cggagcgggt atgactcgcg atgatcataa ggatgtatcg agtcagttat tcgcttccta
      361 ctcaaaggta aaaagcattc gaaacctggc ggcaattatc ggtgaggaag agctttctga
      421 ttccgataaa ctctatcttc ggtttggtga attattcgag cagaaattct tgactcaggg
      481 tgaattcgaa aaccgctcga tagaggaaac cctggatatc ggttggtgtc ttctttcggt
      541 tttgccccgt tcagaactgt atcgcatcaa ggctgagtat atcgaaacct accttcaagc
      601 tgcccttgat aaggatcctt cgttcctttc cggggttaag gcccggatat aagatatggc
      661 cagattaccc gtagccccga ccaagtcaaa ccttctcgcc ataaaggagc aactagctat
      721 tgcccgggat ggttatgaat tgttggaaca aaaacgggag atactcgtta tggagcttat
      781 gcagatggtc gaaaaggtta agctccttga ggcggatata gagaaacaaa ttgataaggc
      841 ctatccatcc ctaaaaagaa tgtttatggc cgtaggtcgc gaccggacag aacgggtatc
      901 acggacggta tcctatgact ttcatctggt tgagaaacgt gtcgtgatgg ctggtatgaa
      961 cttttcgaca ctttctgtcg aattgccaaa acgtcggctt gcctggtcat atttaaattc
     1021 atttgccgat tgcgataaag tcatgataga ctttttcgag cttctacgcc tattaactga
     1081 tatggcttcc atcaggacca ttgtgtggcg gttggcacga gaagtaaaaa aaacgcaacg
     1141 gcgcgtgaac gccctcgata aacaggtgat cccgcaaaca cgcgaaacca gctcctatat
     1201 agaaagtgtc cttgaagaac gcgagcgcga gaatgtgttc gttcttaagg cactcaaggc
     1261 aaaaaacgga ggcctttcga gatgatcaaa ccgcttttcc agaacattct cgtactgctc
     1321 aatggaaccg atgcttcaat caacgcggtg cggtatgccg tccttatggc aaaactgtat
     1381 cgtagcaggg tatacgcggt ctatgttg
//
LOCUS       DAOQXQ010000538         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_118104_length_1406_cov_3.838638, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000538 DAOQXQ010000000
VERSION     DAOQXQ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118104_length_1406_cov_3.838638"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PKH81_08370"
     CDS             <1..1350
                     /locus_tag="PKH81_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HNQ98091.1"
                     /translation="IGAGGAGMAAGVSAHQNGATVIILEKMPKVGGNTISAGGAMNAV
                     DDGSETALKNKDSVALHYKRTFEGGDKKGDEALVKTLVENAWDSVAWLQSMGMKFLPD
                     PFTVTGGLWPRAHKPVDPVGTGFFKTYNAYIESNKGIEVRLNVKADSIILENGKVTGV
                     IATGKTGNKITVKANKGVVVATGGFGQNIAMRQEFNKAWPTLDESVKSTNHPGATGDG
                     IAMAKAVNAQLVQMENIQLLPLGDPETGSLSGNIETDVEKRIFVNTEGKRFVDEGARR
                     DTMTLALFKQPGAKLWIIVDAHSYPQGTKKNNFNESIDDLVAAGRAFKGETLEELAKN
                     IGVPPENLVATVADFNKHVASKKPDAFGRTLFSEPLDKGPFYAGARIPTVHHTMGGIK
                     IDKETHVIDVNGQIIPGLYAAGEVTGGIHGTNRLGGNALTDITVFGRIAGKNAAAGK"
BASE COUNT          409 a          346 c          365 g          286 t
ORIGIN      
        1 attggagcgg gcggtgctgg aatggccgca ggcgtttcag ctcaccaaaa cggcgcaacc
       61 gttattatcc ttgaaaaaat gcccaaggta ggaggaaaca ccatctctgc cggtggtgcg
      121 atgaatgctg ttgatgacgg tagcgagaca gcgcttaaaa acaaagactc agtagccctt
      181 cattataagc gaacctttga aggtggagat aaaaagggag acgaagcgct tgttaaaacc
      241 ctggtagaaa acgcatggga ctcggtcgcc tggcttcaat ccatggggat gaagttcctt
      301 cctgacccct ttaccgttac cggaggtctg tggcctcgcg cgcataaacc ggtcgacccc
      361 gtaggaaccg gcttttttaa aacctacaat gcgtatatcg aatcgaacaa aggaatcgaa
      421 gtcaggctca atgtaaaggc cgattcgatc atccttgaga atggaaaggt tactggcgtt
      481 atcgcaaccg gcaaaaccgg aaacaagatt accgttaaag ccaataaggg tgtcgtcgtc
      541 gcaaccggtg gttttggtca gaacatagcc atgagacaag agtttaacaa ggcttggccg
      601 accctggacg aaagcgttaa aagcactaat caccccggcg caactggaga cggaattgcc
      661 atggcaaagg cggtaaacgc tcagctggtg cagatggaaa acatccaact tctgcctctg
      721 ggagacccgg aaaccggaag cttgagcgga aacatagaaa ccgacgttga aaaacgaatc
      781 tttgttaata ccgaagggaa gcgctttgtt gacgagggag ctcgtcgcga caccatgacg
      841 cttgccctct ttaagcagcc tggtgcaaaa ctctggatta tcgtggatgc gcactcgtat
      901 ccccagggaa caaagaaaaa taactttaac gaatccatcg acgaccttgt cgccgcaggc
      961 agggccttta agggtgaaac ccttgaagaa cttgctaaaa acatcggggt acccccggag
     1021 aacctggttg ctaccgttgc cgactttaac aagcacgtcg catccaagaa gcccgatgcc
     1081 tttggacgga cactcttttc agagcccctt gataaaggcc ccttctacgc tggcgctcga
     1141 attcccaccg ttcatcacac catgggagga atcaagatcg acaaagaaac ccatgttata
     1201 gatgttaacg gccagattat tcccggcctg tacgcagcgg gagaagttac tggcggaatt
     1261 cacggaacca accgtctagg tggaaacgcc cttacagata taacggtatt cggaagaatt
     1321 gccggaaaga acgcagcagc gggtaagtaa aaaaaacgag tactccataa aaagagccct
     1381 tgtgcatctc gcgcaagggc tttgta
//
LOCUS       DAOQXQ010000539         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_118578_length_1403_cov_2.764095, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000539 DAOQXQ010000000
VERSION     DAOQXQ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118578_length_1403_cov_2.764095"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKH81_08375"
     CDS             <1..637
                     /locus_tag="PKH81_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98092.1"
                     /translation="HRLGLLYYHNGEHSGGMYYSGQINESLLPYMEGAISSSSLVRSL
                     DGSTLTQRSDGWSADFLAGMSYAPSFADVSVFLEYRYRSSGYSESDWKNIEIALSSLH
                     IDRPYDYYPVCGYLSTTIPYLETAIHAVGLRIQNTGLVAGFLNYSLTSFFLAPDGFYS
                     AARIELHAIDRLSLAVQGVSIVSVDRAGETAWWNNTWNIQIAARWTVRAYE"
     gene            663..>1403
                     /locus_tag="PKH81_08380"
     CDS             663..>1403
                     /locus_tag="PKH81_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98093.1"
                     /translation="MSKELVVALRSLIAHRRKTWAIVASVAMGICALVLVGGYYEYNF
                     WGLKQALIRSQYAHLQLYQKGYLGERDVSPFTLPISDSSGLIELLEKKSSVITAAPRS
                     RAMGMINGHPVEIWGVDPDREARIFTFTTAKRGVTLLSSDRTSCQISPRLASNIGVAL
                     NQSVTLMAIRQDGMVNALELKVQSLIGSYAEDFDQMVVIVPKDTFIDLFGTDMVHEVV
                     VLLPDDTPLPAYRRTLQAELRKAGYDLDI"
BASE COUNT          361 a          282 c          344 g          416 t
ORIGIN      
        1 acaccgtttg ggtcttttgt attatcacaa tggagaacat tccggcggga tgtattattc
       61 cggacaaatc aatgagagct tgcttcctta tatggaaggc gcaatctcaa gttcatctct
      121 cgttagatca ttggatggaa gtactcttac gcaaagaagt gatggatgga gtgcggactt
      181 cttggccgga atgtcatatg ctccttcctt tgcagatgta agtgtgtttc tggagtatcg
      241 ataccgatct tcggggtata gcgaaagtga ctggaagaat attgagatag ctctatcgtc
      301 gttacacata gatcgcccgt atgactatta tccagtttgc ggctatctgt ctacaactat
      361 tccgtatctg gaaaccgcta tccatgcggt aggcctgagg attcaaaaca ctggacttgt
      421 agcgggtttt ttgaattata gtcttacttc gttttttctt gcaccagatg gattctatag
      481 tgctgccagg atagagcttc atgccattga ccgactgagc cttgctgttc aaggggtttc
      541 aatcgtgtct gtggacaggg ctggagaaac agcctggtgg aataacacct ggaatataca
      601 aatcgcggct cgctggactg ttagagctta tgagtaaggt cattatgacc gggagattgt
      661 ctatgagtaa ggaactggta gtagcactaa gaagtcttat tgcacaccga cgaaaaacct
      721 gggcgatagt tgccagcgtt gctatgggaa tttgcgctct tgtattggtt gggggctatt
      781 atgaatacaa tttttgggga ctcaaacaag cccttatcag aagccaatac gcgcatctgc
      841 aattatatca aaagggatac cttggcgaac gggatgtttc gccttttacc ttgccgatat
      901 ccgattcatc tggattgata gagctgcttg aaaaaaaatc ttcggttatt acagcggctc
      961 cccgatctcg tgctatggga atgataaacg gacatcctgt tgagatatgg ggagttgatc
     1021 cagatagaga agcaagaatt tttaccttta ctaccgcaaa acgcggcgta actttacttt
     1081 cttctgatag gacatcgtgt caaatatcac cgcgacttgc atctaacatt ggcgttgcgc
     1141 ttaatcagtc tgttacgctt atggctatcc ggcaggacgg gatggtaaat gcccttgagc
     1201 tcaaggttca atcgttaatt ggtagttatg ccgaagattt tgatcaaatg gtagtgattg
     1261 ttccaaaaga tacctttata gatctttttg gtacagacat ggtccatgag gttgttgtgt
     1321 tactgcctga cgataccccg cttcccgcat atcggcgaac gcttcaagca gaactacgaa
     1381 aggccggata tgatcttgat att
//
LOCUS       DAOQXQ010000540         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_118732_length_1402_cov_2.556050, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000540 DAOQXQ010000000
VERSION     DAOQXQ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118732_length_1402_cov_2.556050"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PKH81_08385"
     CDS             complement(<1..1389)
                     /locus_tag="PKH81_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98094.1"
                     /translation="MDHFVQFGDMFTGIRDILQVKIQSDILQAERSLGNELALKVLKA
                     LFLVKYVKGFHSTVDNITKIMLPTFHVDFIEFKEQIQVALNKLEQQSYIERAAGDEYH
                     FLTNEEKDIENAIKSTDLAPDAAGKELSKILFTEIYSEPKIRINNNTRLNYSYGRFID
                     EVQDGREQDFYLHFFTPMAENQYTDSATLLIKSMQYSNHLIVALGEDKTLGRDLIMYL
                     KAEKCLNFIQSAAADEYKRQIIIDKRKLNAERRREVVLRMQELISDARLYIAGQELTD
                     ITARDIRLRISGGLQQLVTSVYTNLRLLTKDYDENTLRTICNDSHATDAFGYQLEECD
                     SEVLGFINRNKQNSVRSTVKGVVDLFKSKPYGWYDLATVCILAKLFKMNKLSFRYDGS
                     MLEDRDLVNYLTASARQANIIIDLEEAIPESQVRKLKTLFQNYFGRPCTQTEAKAIHK
                     EFIEALAVVTAEL"
BASE COUNT          368 a          311 c          281 g          442 t
ORIGIN      
        1 aagctcagcg gttactacag caagtgcctc tataaactct ttatgaatcg ctttagcctc
       61 cgtttgagta caaggtctgc caaaataatt ctggaaaagg gttttcaatt tgcgcacctg
      121 cgattcgggg atggcttcct ccaagtctat tatgatattt gcctgacggg cagatgctgt
      181 aaggtagttt accaaatcgc gatcttccag catagagcca tcgtaccgaa acgaaagctt
      241 attcatctta aagagcttgg ccagaataca aaccgtagcc aggtcgtacc agccgtatgg
      301 tttcgatttg aataaatcta ccaccccttt taccgtgctt cgcacactgt tttgtttgtt
      361 gcggtttata aaaccaagca cttcgctgtc gcactcttcc agctgatagc caaaagcatc
      421 ggttgcgtga ctatcgttac atatggtgcg gagggtattt tcgtcgtaat ctttagtaag
      481 cagacgtaaa ttggtatata cacttgttac caactgttgc agacctcccg aaatacgaag
      541 gcgtatatcg cgtgctgtga tatctgtaag ttcttgtccg gcaatataca aacgtgcgtc
      601 cgagattaat tcctgcatgc gaagtacaac ctcgcgacgg cgctcggcat ttagcttgcg
      661 cttgtcaatt ataatttgcc ttttgtactc gtcggctgca gccgactgaa tgaaattcaa
      721 acatttctct gcttttaaat acattatcaa gtcgcgtccc aacgttttgt cttcgcccaa
      781 agctacaatc aaatggtttg aatattgcat ggactttatc agcaaagtgg cactatccgt
      841 atattgattt tctgccatgg gggtaaagaa atgcagataa aaatcttgtt cccttccgtc
      901 ttgcacctca tcgataaatc gtccgtatga atagttaagc cgggtgttgt tgttaatgcg
      961 aattttgggt tcactgtata tttcagtaaa caagatttta gacagctctt tccctgctgc
     1021 atccggagca agatcggtac ttttaatcgc attctctata tctttctctt cgttggtaag
     1081 aaagtgatat tcgtctcctg cagctctttc gatataggat tgttgctcga gtttatttaa
     1141 tgcaacctgt atctgttctt tgaattcaat aaaatctaca tggaaggtag gcaacataat
     1201 tttagtgata ttatctaccg tagagtggaa tccttttaca tatttaacca ggaatagcgc
     1261 ctttagcacc ttcaatgcca actcgttacc caatgagcgt tcggcctgca gaatatcgct
     1321 ttgaattttt acctgcagaa tgtcgcggat acccgtaaac atatcgccaa actgaacaaa
     1381 atggtccaaa tccttgtctt ta
//
LOCUS       DAOQXQ010000541         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_118967_length_1400_cov_4.488476, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000541 DAOQXQ010000000
VERSION     DAOQXQ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118967_length_1400_cov_4.488476"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1324)
                     /locus_tag="PKH81_08390"
     CDS             complement(143..1324)
                     /locus_tag="PKH81_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNQ98095.1"
                     /translation="MTDKIIEINEEKVKDHLGEFVRNTVEETLNAMLEEEATAICKAQ
                     KYERTESRRGTRAGRYERKLLTKSGEVTLKVPKLRKVTFETAIIERYKRREISVEEAM
                     IEMYIAGVSVRRVEDITEALWGTKVSPGTISNLNKKIYEQIERWRNEPLKTHYTYVYL
                     DGIWLKRTWGGEVKNVSVLVAIGVNEEGFRDIIGVAEGFKEDKESWKRFLQYLKGRGL
                     ERIDLIISDKSLGLIETIPDFYPRAKWQRCMVHFYRNVFSLVPHGKVKEVATMLKAIH
                     AQENIEEARRKKDMVADKLVEMKLGKAAEIVRDGCVETFSYYRFPVEHWKRIRTNNGL
                     ERIMKEIRRRTRVVGAFPDGESALMLVAARLRHVASSTWSSRKYLNMTLLNDIDIDSV
                     A"
BASE COUNT          343 a          364 c          244 g          449 t
ORIGIN      
        1 aaattttgtg gttattattt aaatcgtcag atcgaggact tttttattat ctaaaagtgc
       61 gaaagatatt ttacactacc aacctgggta acgtcaataa tttttcgcac attcaaagtc
      121 ctcgatcatc cgacctaata ccctaagcaa cagaatctat atcgatatca ttcaataaag
      181 tcatattcaa atactttctt gagctccagg tcgatgatgc cacgtgacgc agtcgggcag
      241 cgaccagcat aagggccgac tcaccatcgg ggaaggctcc aacgaccctg gttcgacgcc
      301 gtatctcttt catgatccgc tcaagaccgt tattggtcct gatccgtttc caatgttcaa
      361 caggaaatct gtaatacgaa aacgtttcca cgcatccatc acgaacgatc tcggcagcct
      421 tgcccagttt catctcgacg agtttatctg cgaccatatc cttcttgcgc ctggcttcct
      481 cgatgttctc ctgggcatgg atcgccttga gcatcgttgc aacctcttta accttgccat
      541 gaggaacaag tgaaaagacg tttcgataaa aatgaaccat gcaccgttgc catttcgccc
      601 tgggatagaa atccggtatt gtttcgatca ggcccaatga tttgtccgat ataatcagat
      661 cgattcgttc cagaccacgg cctttcaaat attgtaaaaa ccgtttccag ctctccttgt
      721 cctccttaaa tccttctgca acgccgatta tatcgcgaaa tccttcttca ttgaccccta
      781 tagcaaccaa tactgataca ttttttactt caccgcccca ggtccgtttc agccatatcc
      841 catcaagata cacgtacgta taatgggttt ttaaaggctc attacgccat ctctcgatct
      901 gttcatagat tttcttgttt aaattgctga tcgtccccgg tgataccttg gttccccata
      961 aagcttcggt aatatcttca actcgacgaa ccgatacccc ggctatatac atctcgatca
     1021 ttgcttcttc tactgatatt tctcgtctct tataccgttc aatgatcgcg gtttcaaatg
     1081 ttactttccg tagcttggga actttcagag taacttcgcc acttttcgtc aagagcttgc
     1141 gttcgtaacg cccggctcgt gttcctctgc ggctttctgt tcgttcatac ttttgtgcct
     1201 tacagattgc tgttgcctct tcttccagca tggcgttcaa ggtctcctca actgtattcc
     1261 ggacaaattc ccctaaatga tccttgactt tttcctcatt aatttcgata atcttatcag
     1321 tcatacaagt cctcctctga gagttaaatt ttgtggttat tatttaaatc gtcagatcga
     1381 ggactttttt attatctaaa
//
LOCUS       DAOQXQ010000542         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_119150_length_1399_cov_2.784226, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000542 DAOQXQ010000000
VERSION     DAOQXQ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119150_length_1399_cov_2.784226"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PKH81_08395"
     CDS             complement(<1..>1399)
                     /locus_tag="PKH81_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNQ98096.1"
                     /translation="WKFISALRFGLLDIYKQQICLLHVRPANESYVIEAIKTVNMCET
                     DRISETIVPGMVSITQTRLLPFLQGQQIFVWDESAQQKQLTHIFGKGVEFNLLDLRPQ
                     IGSVIPQVKSLSLLKLKDLSRIGRYQQKSIGELEGFFNLFISFLQRKNGLYGKRETEE
                     LQLVALGTLADLMPLKNENRILVRLGLAAMNEKPRAGISELLSRQNLLGKKIGTQELA
                     WQISPAINATGRMGNPGLAIRLFLSTDPTERTALSAEVIQLNTDRKQMGSDGWTVVEP
                     LARESFERFNSKLVVAATNLVHRGVTGIMANRLANCFSVPAIVICIMDDGSAVGSIRS
                     ARGFHLEGLLAPCADLFTDHGGHAFAAGFSLPQTKLEQLISRLERVSQDITFEGEGND
                     EEILVDAELPHEYLQASLLDIADRFEPWRRQRTLGIPFAQDARYLRGHHGQNRKATSK
                     TDTGLWHSQVASHFLA"
BASE COUNT          347 a          323 c          352 g          377 t
ORIGIN      
        1 tgccaaaaaa tggctggcca cttgtgagtg ccacagtcca gtgtcagttt tagatgttgc
       61 ttttctgttt tgcccatgat gtccgcggag ataacgcgca tcttgcgcga aaggaatacc
      121 aagggttcgt tgtcttcgcc atggctcaaa gcggtcggcg atatcaagta atgaggcttg
      181 aaggtattca tgtggtaatt cagcgtccac caggatctct tcatcattgc cttcaccttc
      241 aaaggtgata tcttgcgaaa cccgttcaag acgcgatatc aattgttcta gtttggtttg
      301 agggagggag aaccccgctg caaaagcgtg tcccccatga tcagtaaaaa ggtctgcaca
      361 gggagcgagt aatccttcca gatggaagcc gcgggccgac ctgattgaac cgacggcaga
      421 gccatcgtcc ataatgcaaa taacaattgc tgggaccgaa aaacagtttg ccagccgatt
      481 tgccattata ccggtaacgc cccggtgtac cagattggtt gccgcaacaa caagtttaga
      541 gttaaatcgc tcaaaacttt cccgtgccag gggttccacc acagtccatc catcactgcc
      601 catttgcttg cgatcggtgt taagctgaat aacctcggca gaaagggcgg tgcgttcggt
      661 tggatcggta gaaaggaata atcgaatggc aaggccggga ttgcccatcc gaccggtggc
      721 gttaatggca ggagatattt gccatgcaag ctcttgggtg ccaatctttt ttccaagcag
      781 gttttgacgt gacaatagct cggaaatacc ggctcttggt ttttcgttca tcgccgcaag
      841 tcccagtcga accaggatgc ggttttcgtt ttttagcggc atcaggtcgg caagggttcc
      901 cagggcaacg agttgcaatt cttctgtttc gcgctttccg tataaaccgt tttttctttg
      961 gagaaatgaa ataaacaaat taaagaatcc ctcgagctca cctatgcttt tttgctgata
     1021 gcgaccgatg cgagagaggt ctttgagttt taacaagctg agggatttta cttggggtat
     1081 caccgatcca atttgtggtc ttagatcaag cagattgaac tcgactccct ttccaaagat
     1141 gtgtgtaagc tgcttttgtt gtgcactctc gtcccacaca aagatttgct gcccctgcaa
     1201 gaagggcaga agccttgttt gtgtaatgga taccataccg ggaacgattg tttcactaat
     1261 gcggtcggtt tcgcacatgt taacggtttt aatggcttcg ataacatacg actcatttgc
     1321 ggggcgtaca tgcaacaggc agatttgctg tttgtatatg tcaagtaagc caaagcgcag
     1381 ggcagaaata aacttccac
//
LOCUS       DAOQXQ010000543         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_119597_length_1396_cov_2.668158, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000543 DAOQXQ010000000
VERSION     DAOQXQ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119597_length_1396_cov_2.668158"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PKH81_08400"
     CDS             <1..1240
                     /locus_tag="PKH81_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNQ98097.1"
                     /translation="WREEELDEHRIVYYESSRGCPYSCSYCLSSIEKSVRFLSLERVY
                     ADLAWFMEKRVPLVKFVDRTFNLDRRRYQAIWEYIAQNWNGSTVFHFEIAGEILDEDS
                     YSIMERIGEGAIQFEIGIQSTNARTLTACGRSANTEMLFKNIRKIPRTIHCHVDLIAG
                     LPYEDLASFERSFNEVWALEADMLQLGFLKILSGTSMESFALQTKGYRWSRHCPYEVM
                     ESPWLTREELYLLKDVEHLVDGWFNSSLARNTLLFLAQECSSSAFTLFVRLAAFVRSF
                     FPDGDLFLPRRPSAAFECLAAFIATLSPIAAASALERLKLDFLLLGKPGTVPAWFIRR
                     YSRDAHDAALTAQGLLDAAEGAHAPSRRTVYARSEWEALDLDGKDGVRSGYLFVYPEA
                     GGSKKVSGSTIMYYTGAIRS"
BASE COUNT          302 a          345 c          373 g          376 t
ORIGIN      
        1 ctggcgggag gaagaactgg atgaacatcg catcgtctat tatgagtcaa gccggggctg
       61 cccctactcc tgctcgtatt gcctttcttc tatagaaaag tcggttcggt ttctgtcttt
      121 ggagcgggtg tatgcggacc ttgcctggtt tatggaaaag cgggtgccgc tcgtaaaatt
      181 tgtggaccgc acctttaacc tggatcgccg ccgctaccag gcaatctggg aatatatcgc
      241 gcagaactgg aacggtagca cggtgtttca ttttgagatt gcgggggaga tactggacga
      301 ggacagttat tcgataatgg agcgaatcgg cgagggcgcc atccaattcg agatcggcat
      361 acagagcacc aatgcgagaa ccctaaccgc gtgcggccgt tccgcgaaca cagagatgct
      421 ttttaaaaat atccggaaaa ttccgcgaac catacactgt catgtggatt tgattgcggg
      481 ccttccatat gaagaccttg cgagctttga gcgctccttt aacgaggtgt gggctctgga
      541 agcagatatg ctccagcttg gttttttgaa aattttaagc ggtacgtcca tggagagctt
      601 tgccttgcaa acaaaagggt atcgctggtc ccggcactgc ccctacgagg ttatggaatc
      661 tccctggctt actcgcgagg aactctacct tttaaaagac gttgagcatc tggtggacgg
      721 ctggtttaat tcatcccttg cgcgaaatac tcttttattt cttgcgcaag agtgctcctc
      781 ttccgccttt actctgtttg tccggttggc ggcctttgtt cgctcctttt tccccgacgg
      841 ggatctgttc cttccccgaa ggccaagcgc cgcctttgaa tgtcttgccg cctttatcgc
      901 gactctttcc cctatcgctg cggcttctgc ccttgagcgg ctaaagttag actttttgct
      961 tttgggaaag cccggaaccg ttcctgcctg gtttatccgc cgctattccc gggatgcaca
     1021 cgatgcggct ctgactgctc agggcttact ggatgcggcc gaaggcgccc atgcaccgtc
     1081 ccggcgcaca gtctatgcgc gaagtgaatg ggaggccctt gatcttgatg gcaaggacgg
     1141 ggttcgttcc ggctatcttt ttgtgtatcc ggaagctgga gggtcaaaaa aagtttcggg
     1201 aagtacaatt atgtactata ctggagcaat caggagttaa aacaatggac gtaacaataa
     1261 taaacccgtt tttaagcgcg acaatcaatt tgtttcaaaa tatgttttct atcgacgcgc
     1321 aggctggcac gccctattta ctggaagggg aaattaaaca ccgttgggag atatccggca
     1381 ttcttgggct taccgg
//
LOCUS       DAOQXQ010000544         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_119995_length_1393_cov_2.627803, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000544 DAOQXQ010000000
VERSION     DAOQXQ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119995_length_1393_cov_2.627803"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKH81_08405"
     CDS             <1..218
                     /locus_tag="PKH81_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="K+/H+ antiporter"
                     /protein_id="HNQ98098.1"
                     /translation="ALAVLTYRSTAAVGGSGFLAVYIAGLVAGNSEFIYKKSMLRFFD
                     GLAWLSQIAMFITLGLLVFPSRIISII"
     gene            243..464
                     /locus_tag="PKH81_08410"
                     /pseudo
     CDS             243..464
                     /locus_tag="PKH81_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            573..794
                     /locus_tag="PKH81_08415"
     CDS             573..794
                     /locus_tag="PKH81_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNQ98099.1"
                     /translation="MIPFSAAISGKPIVEIGLPRDSLIVLINRNENFLVPSGGTVLEE
                     GDVVLVLVNQKNLAEVRETLMRWKKPEEG"
     gene            1083..>1393
                     /locus_tag="PKH81_08420"
     CDS             1083..>1393
                     /locus_tag="PKH81_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98100.1"
                     /translation="MARTSQQAGGKGRPSQQTKLSQKGLPPQTCISHEISVIGEFHGY
                     RFNNGNFAVLKAKIISVNSNGDGTGGTRPSGLHTIFTIVSPHLDPFVEVLDEGIDEEF
                     Y"
BASE COUNT          351 a          333 c          333 g          376 t
ORIGIN      
        1 tagctttggc ggtacttacc tatcgttcaa cggcggcagt cggaggaagc ggttttcttg
       61 ctgtttacat cgcgggcttg gttgccggca acagtgagtt tatctacaag aaaagcatgt
      121 tgcgattctt tgacggcctt gcctggctca gccagatagc tatgtttatc acccttggcc
      181 tgctcgtttt cccttccaga ataatctcaa tcatttaggc cggtttagcc tgttcagcct
      241 tcctcatgct tgtagcccgt ccgataagcg tttttctttc tttgctcttt gtgcagctta
      301 gatggcggga aaaggtcctt gtttcctggg ttggcctgag gggcgctgtc cctatagttc
      361 tggcgacttt cccactcgtt gccggtttgg ctgaagccga gtcgatattc aatatcgttt
      421 tctttatcgt cctaacttcg gccctgctcc aaggctggtc gattcccgct gtcgcacgat
      481 ttttaaggct ccaggcgccg atcatcccaa agcggcaata ccctatcgag tatgctccgg
      541 tagatggggt tgatactcaa cttgtcgaat tcatcattcc cttcagcgca gccatttcag
      601 ggaaaccaat agtggaaata ggtcttccac gcgacagcct cattgttctc ataaaccgca
      661 atgaaaactt tttagtgcca agcgggggta cggttcttga ggaaggtgat gtcgttctgg
      721 ttcttgtcaa tcaaaaaaat ctggcagaag tccgggagac cctaatgcgg tggaagaaac
      781 ccgaagaagg ataatcggaa aaaatctgat ttgccgatag gaagaaaatc ttatcgaaac
      841 aacataatta acccttgccc cttacttttt ctcccgaacg ggaagagatc aaaggcagat
      901 ataattaagg cttgcagccg tatctttgca tcggaaggtc agaggccagg gtctgatctc
      961 cagcatgagc tgtgaaggca tatggtagat aaatacagcc ttattgccgt tgaagatttt
     1021 aaaaaatcta ccgtggttcc catgtatttt tcatgggaac aaaatacggt aatacaggat
     1081 aaatggcaag gacatcgcaa caagcgggag gaaaagggag accttcgcag caaacaaaac
     1141 tttcgcaaaa aggattgcct ccgcaaacct gtatttccca tgagatctcc gtcataggcg
     1201 aattccacgg ctaccggttt aacaacggca atttcgcggt cctgaaagcc aaaattatca
     1261 gcgtcaactc caacggcgac ggtacaggcg gtactcgccc ttcgggcttg cacacaatat
     1321 tcacaattgt atcaccgcac cttgatccct tcgtcgaggt acttgatgag gggatagatg
     1381 aagaattcta tga
//
LOCUS       DAOQXQ010000545         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_120574_length_1389_cov_2.940030, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000545 DAOQXQ010000000
VERSION     DAOQXQ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120574_length_1389_cov_2.940030"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="ychF"
                     /locus_tag="PKH81_08425"
     CDS             <1..900
                     /gene="ychF"
                     /locus_tag="PKH81_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNQ98101.1"
                     /translation="VDIAGLVKGASKGEGLGNQFLSHIREVGVIAQVVRCFENPDIIH
                     VNNAVNPASDIETINIELALADLETLDKRSERASRASKVQGKDVQKEADMVLGAIAKI
                     RPLLQEGKPARSAPLSDEEKAAIYDCHLITMKPQMYVCNVDEEGMKNGNAHIETVKAI
                     AAGEGADVVVICGKFESELADLESQEEKLAFLEEIGLTESGLTSLARAAYHLLGLRTF
                     FTAGEDECRAWTIRAGDTAPKAAGVIHTDFEKGFIKAEVYSYDDFVKYGSEQKIREAG
                     RYRQEGKEYQVADGDIIFFKFNV"
     gene            912..>1389
                     /locus_tag="PKH81_08430"
     CDS             912..>1389
                     /locus_tag="PKH81_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNQ98102.1"
                     /translation="MKRSVLNLGCALAGFITLVGMSSCGQKPVSSVPEGMTLVWSDEF
                     DTDGAPDSEKWDYSIGGNGWGNGEAQKYTNTRENSIVKKGNLVITALYDGLWSSARLK
                     TQFKARWTYGYFEIRAKLPRGVGTWPAIWMLPEFDKYGMWPRSGEIDIMEHVGFDED"
BASE COUNT          374 a          291 c          396 g          328 t
ORIGIN      
        1 gtagacatag cgggattagt taagggcgcc tccaagggcg agggcttggg taatcagttt
       61 ttatcgcata tccgcgaggt cggcgttatc gcgcaggttg ttcgctgttt tgaaaatcct
      121 gacataatcc acgtgaataa cgcggtgaat cccgctagcg acattgaaac gatcaatatt
      181 gagcttgctc ttgccgacct tgaaaccctg gataaacgtt ccgagcgggc gagccgggca
      241 tccaaggttc agggaaaaga cgtgcaaaaa gaagcggata tggttcttgg ggcgatcgcc
      301 aagattcgcc cacttttgca agaagggaaa cctgcccgaa gcgctccgtt gagcgatgag
      361 gaaaaggccg ctatctacga ctgccatctt ataaccatga aaccccagat gtacgtgtgc
      421 aacgttgacg aagagggaat gaaaaacggt aacgcacata tagaaaccgt caaagccata
      481 gctgccggag agggcgctga tgtggtggtt atctgcggga aattcgaaag cgaactggcg
      541 gacctggaaa gccaggaaga aaagctcgcg tttttggaag aaatcgggtt aacagaatcc
      601 ggtttgacga gtcttgcccg tgcagcctat catttactgg gtttgcgtac tttttttacc
      661 gccggagaag atgagtgccg tgcctggact attcgcgcag gagataccgc accaaaagcc
      721 gcaggtgtta ttcacacgga ctttgagaaa ggctttataa aggcagaggt gtatagttac
      781 gatgactttg taaagtacgg aagcgagcaa aaaattcgcg aggccgggcg atatcgacag
      841 gaaggaaagg aataccaagt cgccgatggc gatattatct tttttaaatt caacgtgtag
      901 gagatcccag aatgaagcgg tctgtactga accttggatg tgcccttgca ggttttatca
      961 ctcttgttgg aatgagttcc tgtggtcaaa aaccggtgtc ctccgttccc gaaggaatga
     1021 ccctggtatg gtccgatgag ttcgacacag acggagctcc ggatagcgaa aagtgggatt
     1081 attctatcgg cggcaatggc tggggtaacg gtgaagccca aaaatacact aatacccggg
     1141 agaactccat tgtaaaaaag ggaaacctgg ttattactgc tttgtatgat gggctgtggt
     1201 cgagcgctcg attaaaaacc cagttcaagg cccggtggac ttatggatac tttgaaatac
     1261 gtgccaaact tcctcgtggc gttggtacgt ggcctgctat ttggatgtta ccggagtttg
     1321 ataagtatgg aatgtggccc cgttcgggcg aaattgacat aatggaacac gttggctttg
     1381 atgaagatg
//
LOCUS       DAOQXQ010000546         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_121386_length_1383_cov_3.647590, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000546 DAOQXQ010000000
VERSION     DAOQXQ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121386_length_1383_cov_3.647590"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKH81_08435"
     CDS             <1..715
                     /locus_tag="PKH81_08435"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNQ98103.1"
                     /translation="FRCDADESGQNSKGELYYGRVRAKVGKLSGASTYRISSPSMVAG
                     VRGTDFGCDHIFVRPQVAGGEGTAEVASTAVVNRVFCFEGSVLVVPVSDPGLKTVLVG
                     GGEMIESSATTASADGSIPVELTKTPVSTEVTEFWKGRQFRGTEHSSLSAEAAQAQIL
                     AEEQLQVVAQEKKRVRNHKVRLAGSAALFGAGALVAGLAYPDYKSDGFTKSVIVNTSY
                     GGALICTSLGLLIYDLIMY"
     gene            860..>1383
                     /locus_tag="PKH81_08440"
     CDS             860..>1383
                     /locus_tag="PKH81_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98104.1"
                     /translation="MKKLFSILVCALAAMYLLAEAPIVMFSVTPQILFPVSPEDEEGD
                     VLYGMGGGASLASVISPRSLRWLSGIIEAGFDYLPTNVTDDSLSLIRLGAGVGVGYQG
                     SKFGARLTGSGGYYYGFYGNTGGGNLYYDGGVQCSYRINEAFTLSLLGGWRDYLSLAD
                     SSETFLVRTPYAGLS"
BASE COUNT          320 a          304 c          358 g          401 t
ORIGIN      
        1 tttccgttgc gacgccgatg agtctggtca aaactctaaa ggcgagctct attatggacg
       61 ggtacgcgcg aaggttggaa agctctcggg tgcctcaacc tatcgcatca gttcaccctc
      121 tatggtagcg ggtgtacgtg gaactgattt tggctgcgat catatctttg ttcgtcccca
      181 ggttgcagga ggtgaaggta cagccgaagt tgccagcact gcggttgtaa acagggtctt
      241 ttgttttgaa ggctctgtgc ttgtagttcc ggtatctgat cctggactta agacagttct
      301 tgtgggaggc ggagagatga tcgaatcatc ggcaacaacc gcttctgctg acgggagtat
      361 ccccgtggaa ctcaccaaaa ccccggtttc tacagaggta acagagtttt ggaaaggacg
      421 acaattccgc ggcacggaac attcttctct ctcggccgag gctgctcaag cacagatact
      481 agccgaagaa cagcttcagg ttgtggcaca ggagaaaaaa agggttcgca atcataaggt
      541 ccgtcttgcc ggatctgcag ccctgttcgg tgcaggggcg ttagtcgcag gtcttgcata
      601 tcccgattat aagagtgacg gatttaccaa aagcgtcatt gtaaatactt cttacggcgg
      661 agcccttatc tgcacctccc ttggcttgct tatctatgat ttaattatgt attaatcgtt
      721 gcgttattta aacattgaac tcccagagta tccgcgatac acttaaggcg gataatcttt
      781 atcgatctta aaaaaagtat agccgcacct aagattctcc taatgatgtg tatcttgcgc
      841 attctttggg gagatgtaga tgaaaaagtt gttttccatt cttgtctgtg ccttggcagc
      901 aatgtatctg ctagcagaag caccaattgt catgttctct gttacaccac aaatcctctt
      961 tcctgtttct ccagaagatg aagaaggaga cgtattgtat ggaatgggag gaggcgcgtc
     1021 tttggcgtct gttatctctc cccgatcctt acgctggctt tctggaatta ttgaagcggg
     1081 attcgattat cttccaacaa acgttactga cgactccctt agcttgatac gtctgggagc
     1141 aggagttggt gtcggctatc agggttcgaa gttcggagct cggttaacgg gatctggcgg
     1201 atattactat gggttttatg gaaataccgg cggagggaac ctgtattatg atggtggagt
     1261 acaatgttcc tatcggataa atgaagcttt taccttgtcg cttcttggag gttggcggga
     1321 ctatctttcc ttggcagact cttccgagac ttttttggta cgaactccgt atgccggact
     1381 ttc
//
LOCUS       DAOQXQ010000547         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_121406_length_1383_cov_2.972139, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000547 DAOQXQ010000000
VERSION     DAOQXQ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121406_length_1383_cov_2.972139"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKH81_08445"
     CDS             <1..657
                     /locus_tag="PKH81_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNQ98105.1"
                     /translation="IKGIRKEKLVFTLDGYTGEFHLSVPGRHNARNAAGAIALAVSLL
                     AEEKRTITSSDLALLRQALDSFKGSKRRCEIIGEAGGVIVIDDYAHHPTAIKTTLEGL
                     KEFYPDRRLVVDFMSHTYSRTASLLEEFASSFAASDELILHKIYASAREQYDGTVDGK
                     TLYEAARKKIKKCSYFEEVLDAREYVAKTLKPGDLLVTMGAGDNWRLGTAFLEDRTIQ
                     "
     gene            654..>1383
                     /locus_tag="PKH81_08450"
     CDS             654..>1383
                     /locus_tag="PKH81_08450"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNQ98106.1"
                     /translation="MKSMTGYAFVETDKNEMTVSIEIKSYNSRFLDLSINQPYWLSRL
                     EIKVREFLSSRIQRGKVECTIRIKEAKSNLQVTADPDAAQAYLSAIRSVAQGIGSNDE
                     VPLSLVIAQEGVLRAERVLDQETYWKRINEALIQAFDLFDKARIKEGHNLEKDILSMI
                     ERIRSSVSLIETCVPEMEKNFKDSIRQKFQDMLGNAVDEQRVLQETAALLVKYTINEE
                     LVRLRSHLDSIAHEFSVNPAPGRKA"
BASE COUNT          416 a          278 c          314 g          375 t
ORIGIN      
        1 atcaagggta tacgcaaaga gaaactcgtg tttacacttg acggatacac cggagagttt
       61 cacctaagcg ttccgggtcg gcacaacgct cgaaatgctg ccggtgctat cgctcttgct
      121 gtatcattat tggcagaaga gaaaaggacg attacttcaa gcgatcttgc cttgcttcga
      181 caagctttgg atagttttaa agggtcaaaa cgtagatgtg aaatcattgg agaagcaggt
      241 ggggttatcg taatagatga ctatgcccat catccaaccg caattaagac aaccttggaa
      301 ggattaaagg aattttatcc cgatcgccgt ttagtcgttg attttatgtc ccatacctat
      361 tcccgtactg cctccttgct cgaagaattt gcatcatctt ttgctgcctc tgatgagctc
      421 attcttcata aaatatacgc ttcggctcgt gaacaatacg acggaacagt cgatggtaaa
      481 accctttacg aagcagcacg aaaaaaaata aaaaaatgct cgtactttga agaagttctt
      541 gatgcacgcg aatatgtagc aaagacgctt aaacctggtg atcttttggt taccatgggt
      601 gctggagata actggcgtct tggcacagcg tttttggagg accgaactat acaatgaaaa
      661 gcatgaccgg ttatgccttc gtagaaacag acaaaaatga gatgactgtt tccatagaga
      721 ttaagagtta taattcacgt tttttagatc tgtccattaa ccaaccatac tggttaagtc
      781 gtctagaaat aaaggttcga gagttcttga gttcccgaat tcaacgcggc aaggtggaat
      841 gtacaattcg cattaaagaa gcaaaatcaa atttgcaggt taccgcggat ccagatgcgg
      901 ctcaagctta tctttcagca atcaggagtg tagctcaagg tattggcagt aatgacgagg
      961 ttccactgtc tctggttatt gcacaagaag gtgtgttacg cgccgagcga gtacttgatc
     1021 aggaaacata ctggaaacgt attaacgagg ctcttataca agccttcgat ttatttgata
     1081 aggcacgtat caaggaaggc cataatcttg aaaaggatat actttccatg atagagcgta
     1141 tacgatcttc tgtttcactc atagaaacct gtgtcccgga gatggaaaag aattttaagg
     1201 attctattcg ccaaaaattt caggatatgt tggggaacgc cgtagatgag cagcgagtcc
     1261 ttcaggaaac tgctgccttg ttagttaaat atacaatcaa cgaggaattg gtcagactcc
     1321 gatcgcattt agattcaata gcacatgagt tttcagtaaa tccagccccc ggacgcaaag
     1381 ctg
//
LOCUS       DAOQXQ010000548         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_121708_length_1381_cov_2.950980, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000548 DAOQXQ010000000
VERSION     DAOQXQ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121708_length_1381_cov_2.950980"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKH81_08455"
     CDS             <1..374
                     /locus_tag="PKH81_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98107.1"
                     /translation="GCGVLYAHKGVVRLLTREEIPETIYGREKFIWLFTQQLTRALEK
                     DGVLGVAKIITDVFTSEPEQAKSLAYRLFTIAERKGWAQEAYAYNSLVIAWQDIQSKA
                     ADLQSQRKGGKQATMFEVQNH"
     gene            389..>1381
                     /locus_tag="PKH81_08460"
     CDS             389..>1381
                     /locus_tag="PKH81_08460"
                     /inference="COORDINATES: protein motif:HMM:NF037501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HNQ98108.1"
                     /translation="MANNHLQVQEGFRVLLFALSDYICKELKQEFGENWWINGVMDVL
                     YEEQKRGLPDSGDWPTLVDSLDVARCLLLFDLHWQRVFRKKLSIDHRTWAKELYGVRN
                     KLAHIGGLDFSDDDAWRALDTMSRLCEQIDPESAEEIRGFLRASRYGTTGGSTTVTEA
                     PSSKEVSVAKKNSGILSSSPINGLPSWRDVITPHPDVAQGRYKNAEFAADLAQVARSE
                     GALEYRDPIEFFARTYMTEGMMGLLDQALRRINGMDGEPVIQLKTAFGGGKTHSMLAL
                     YHLLRGRVSVDKIANIKPVLQRAGVTTLPKANVAVLVGTALDPTRSKRPNTMPGI"
BASE COUNT          354 a          336 c          379 g          312 t
ORIGIN      
        1 ccggttgtgg tgtgctctat gcgcacaaag gcgtggtaag gctattgacg cgagaagaga
       61 tccccgaaac gatttacggc cgcgaaaagt ttatctggct ttttactcag caactcactc
      121 gcgccttgga aaaagacggc gtcctcggcg ttgcgaaaat cataacagac gtttttactt
      181 cagaacctga acaagccaaa tcgttggcgt accggctttt cacgatagcc gaacgaaagg
      241 gttgggcaca agaagcctat gcctacaact cgctcgttat cgcgtggcaa gacatacaga
      301 gcaaggccgc tgatctgcaa tcacagagga aaggcggtaa acaagcgact atgtttgagg
      361 ttcagaatca ttaactgggg ggttgataat ggcaaataac cacttgcaag ttcaggaagg
      421 atttcgggta ctactctttg ctctatctga ctacatatgc aaggaactta aacaggagtt
      481 cggagagaat tggtggataa acggcgttat ggatgtactc tacgaggagc agaaacgcgg
      541 actgccggat tccggcgact ggcctacact ggttgattcg ctcgacgtcg cccgttgcct
      601 cttgctgttc gatttgcact ggcagagagt gtttcgcaag aagttgtcta tcgaccaccg
      661 aacttgggca aaggaacttt acggcgtccg taacaaactg gcgcatattg gtgggttgga
      721 tttcagcgat gatgacgctt ggcgcgcgct cgatacgatg tcgcgtctat gcgaacagat
      781 agatcccgaa agcgcggaag aaattcgggg ttttcttcgc gcttctcgct atggcacgac
      841 aggtggctcc acgacggtta cagaagcacc atcctcaaag gaggtaagtg tggcaaaaaa
      901 gaactccggt atattaagct catcccccat aaatgggctt ccaagttggc gcgacgttat
      961 caccccgcat cccgacgtgg cgcagggaag atataagaac gccgagtttg cagccgacct
     1021 cgcacaggtc gctcgcagcg aaggcgcgct cgaatatcgc gaccccatag agttttttgc
     1081 ccgtacgtat atgacggaag gtatgatggg gcttttagat caggctttac gccgtattaa
     1141 cggtatggat ggcgagcctg ttatccagtt gaagacggcg ttcggaggcg gtaaaaccca
     1201 tagtatgctt gccctttacc atctcctgcg cggcagggtt tccgtcgaca aaattgcaaa
     1261 cataaagcct gttctacaaa gagcgggcgt aacgacattg ccgaaagcga acgtagccgt
     1321 tttagtcggc acggcgctgg atcccacccg aagcaagcgt ccaaacacga tgccaggtat
     1381 t
//
LOCUS       DAOQXQ010000549         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_121724_length_1381_cov_2.634992, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000549 DAOQXQ010000000
VERSION     DAOQXQ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121724_length_1381_cov_2.634992"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PKH81_08465"
     CDS             complement(<1..1026)
                     /locus_tag="PKH81_08465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ98109.1"
                     /translation="MADIPESIGKYKILSLVAKGGMGAVYKAVHPTLKRNVIIKKLTI
                     RGNQVIVERFKREARILMDLDDPRIVHFFDYFKEGTSHYIVLELVDGCSLDSLIRKRR
                     YLSAPLALYIFLEACKALSYAHQKGIIHRDIKPGNILISKTGAVKLADFGIAASEDSE
                     DGDLTKTGTTLGTASYMPPEQFSDSKTVDSRADIYALGVMLYEMVTGKRPFPGNFAPE
                     TLYLIQKGKYPNPAKLNPDIPRIARTLIKKMIQPSPKKRYQNLNRVIRIIERYLHRFS
                     RDEIQKALVAFVARDGVEEPFFKVRARKRVVIPVVLSTVLCIAGICGYAWKAGLIHRT
                     ILSRSYGE"
     gene            complement(1053..>1381)
                     /locus_tag="PKH81_08470"
     CDS             complement(1053..>1381)
                     /locus_tag="PKH81_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="calcineurin"
                     /protein_id="HNQ98110.1"
                     /translation="TFIASHAEPSRFFNQEELIDGRNRNEVLLGLTWTANDEAEEGSV
                     ERMLREYSESAETTVYISGHRPVKGTYALRASGKLVQIHNPSLRVGAIVSPARPFNPD
                     VDIHQW"
BASE COUNT          379 a          325 c          296 g          381 t
ORIGIN      
        1 ctctccatat gagcgagata atatagttcg atgaataagg cccgccttcc aggcatatcc
       61 acagataccg gctatacaaa gaacagttga cagtaccaca gggataacca cgcgttttcg
      121 ggcacgtacc ttgaaaaaag gctcttctac accatctcgg gcaacgaagg ccacaagcgc
      181 cttttgaatc tcgtcgcggg aaaaacgatg aagatagcgt tcgattattc tgataacgcg
      241 atttagattt tgatagcgct tctttggaga tggctgaatc atttttttaa taagggttct
      301 ggcgattcgc ggtatatccg gattcagctt ggcgggattc ggatacttac ccttttgaat
      361 cagatacaag gtctcgggag caaaatttcc cgggaagggt cttttacccg ttaccatttc
      421 gtagagcatc acgccaagcg catagatgtc ggcgcggcta tcaacagttt tagaatcact
      481 aaattgctcg ggaggcatat aggatgcagt cccaagcgtt gttcctgttt tggttaaatc
      541 gccgtcttcg ctgtcttcgc ttgcggcgat accaaaatcc gcaagtttta cagcccctgt
      601 ctttgatata agaatattcc ccggtttgat atcccggtga ataattcctt tttgatgcgc
      661 atacgaaagc gccttgcacg cctccagaaa aatatacagg gccaagggag cacttaagta
      721 ccgtcgtttt cgtatcaaag aatcaagaga acagccgtct accaattcga gcacaatgta
      781 atgggatgta ccttccttaa agtagtcaaa aaaatgaaca atccggggat catctaaatc
      841 catcaaaatt cgcgcttcac gcttaaagcg ctctacaatg acttgattgc ctcgaatcgt
      901 cagttttttg ataattacat ttcgcttgag cgtgggatga acagccttgt acaccgcacc
      961 cattccaccc tttgcaacca aagaaagtat tttatatttt ccaattgatt caggtatgtc
     1021 tgccacagag attcctcctt acatcaaagt tattaccatt gatgaatatc aacatcagga
     1081 ttaaatggac gagcgggact gacgatagca ccaactctca gtgatggatt gtgtatctgc
     1141 acgagttttc cgctagccct gagcgcatac gtacctttta cgggtcgatg cccgcttata
     1201 tacacagttg tttctgcaga ttcggaatat tcacgcaaca tacgctccac agacccttcc
     1261 tccgcttcat cattagctgt ccaggtaaga ccgaggagaa cttcattgcg gtttctgcca
     1321 tcgattaatt cttcttgatt aaaaaaacgg gaaggctctg catgggatgc gataaaagta
     1381 g
//
LOCUS       DAOQXQ010000550         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_122138_length_1378_cov_2.844293, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000550 DAOQXQ010000000
VERSION     DAOQXQ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122138_length_1378_cov_2.844293"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKH81_08475"
     CDS             complement(<1..238)
                     /locus_tag="PKH81_08475"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNQ98111.1"
                     /translation="MYLSVVFNLPLNQSFTYRTMEGETGLLLGKRVEVYLGNRRTTGF
                     VIAESESLDEGCAIDPKNIKEIRRIIDKEAVFGPD"
     gene            complement(247..924)
                     /locus_tag="PKH81_08480"
     CDS             complement(247..924)
                     /locus_tag="PKH81_08480"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ98112.1"
                     /translation="MNAEQKDILYSLLETALCHLSDGKRSFRPSPGFTDDLIHPANEI
                     HDLEGLSQAVTVCELCATAQHTSELREGEGSPAPRVLVVTDAPVQDSSTNQLFTPPQK
                     ALLEKMLAAISLSLSDNTRLTAMTRCYNGSPHFCRPWFDLELALSAPSVLLVLGEAAL
                     SELLHTQQSLDTLRNKALYYQGLPVVVTFHPRDLLLDEQLKRPAWEDLKGLKKILESP
                     GHPDPAV"
     gene            complement(971..>1378)
                     /locus_tag="PKH81_08485"
     CDS             complement(971..>1378)
                     /locus_tag="PKH81_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98113.1"
                     /translation="IFTGFVNWTDLHTYYGMSDIFFTASLSEMHSMTILEALISSLPI
                     VARQDESYLDTVFPGENGYLAQSDQQMKQLTLSLIDDRDAREAFGKASADIAKRFSIE
                     THTKRTVAFYQAVLDSWPHQLDEDELHRRIALV"
BASE COUNT          354 a          312 c          367 g          345 t
ORIGIN      
        1 gatcaggccc aaacacggct tccttgtcga ttatccttct gatttccttg atattcttgg
       61 gatctattgc acaaccctca tcgagggatt cgctttctgc gatgacgaac ccggtcgtac
      121 gtcgattgcc cagatacact tcaacccgct tgccaagcag caacccggtt tctccttcca
      181 tcgtacggta ggtaaaggat tgattgagcg ggaggttaaa cacaacggaa agatacatca
      241 caagtcctac acagcgggat cgggatggcc aggtgattca aggatttttt ttaatccttt
      301 taagtcttcc catgcaggtc gtttgagctg ttcatcgagc aacaggtccc ggggatgaaa
      361 ggttactact acgggaagcc cttggtaata cagggccttg tttcgcaggg tatcaagcga
      421 ttgttgtgtg tgtaaaagct ccgaaagggc agcttctccc aggactaaaa gaaccgaggg
      481 cgcgcttagg gcaagttcaa gatcaaacca gggacgacag aaatgaggcg atccattgta
      541 acagcgtgtc atggccgtaa ggcgggtgtt atccgaaagg gaaagggata tcgccgcaag
      601 cattttttcc agaagggctt tttgtggagg ggtgaataat tgattcgtgg aagaatcttg
      661 aactggagca tcggttacaa cgagtactcg cggagcggga cttccttctc cttcgcgaag
      721 ttcactggtg tgttgtgctg tcgcgcacag ctcgcataca gtaacagcct gggacaagcc
      781 ttcaagatcg tgaatctcgt tagcgggatg aataaggtca tcggtaaatc ctggagaagg
      841 acgaaaggag cgcttgccat cgctaagatg acacagggcg gtttcaagga ggctatacag
      901 gatatctttt tgctcggcat tcacactgca gaggatatcc tatggctcct tttttggcaa
      961 ccgagatgcc ttataccaag gcgattctcc gatgtaattc atcttcgtca agttgatggg
     1021 gccatgagtc caaaactgcc tgataaaagg ctacggttcg cttggtatgt gtttcaatcg
     1081 agaatcgttt ggcaatgtcc gcgcttgcct tgccaaaggc ttctctggca tcccgatcgt
     1141 caatcagaga aagggtaagc tgcttcatct gttgatcact ttgggcaaga tagccatttt
     1201 ccccggggaa cacggtatca agatagcttt catcctggcg ggcaacgatg ggcagggagg
     1261 atataagagc ttccaggatc gtcatggaat gcatctccga cagggaggcg gtaaaaaaga
     1321 tgtcgctcat accataatag gtatgcaaat ccgtccaatt aacaaaaccg gtaaagat
//
LOCUS       DAOQXQ010000551         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_122268_length_1377_cov_2.925113, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000551 DAOQXQ010000000
VERSION     DAOQXQ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122268_length_1377_cov_2.925113"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKH81_08490"
     CDS             complement(<1..527)
                     /locus_tag="PKH81_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ98114.1"
                     /translation="MKTQLIKDILVSAPDGREVTVYGWVRTKRETKNLVFIQVNDGSC
                     FASIQVTLDRDRGITEETEAQLKRISTGASIEATGLLIPSPASGQAVEIQGLAITVLG
                     EAPVETYPLQKKNHSLEFLRDIAHLRPRTNTFGAVARMRSQMAYAIHVFFQERGFNYV
                     HTPIITASDCEGAGE"
     gene            727..>1377
                     /locus_tag="PKH81_08495"
     CDS             727..>1377
                     /locus_tag="PKH81_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNQ98115.1"
                     /translation="MQYEGASASYFESLSRLTEQNDCIDPELYVKYDVKRGLRDSDGK
                     GVLVGLTRIGNVYGYEVVDGKPVAVPGKLIYRGYDVEDIINDIEKSEQFGFEQVIYLL
                     LFGELPNASQLAEFVALLGEFRELPQNFVEDMILKSPSSDIMNKLARSVLVSYSYDPN
                     PEDVSIANNLYQCIQLIARFPAMVAYGYQAKRRHYDGKSMYLHNPLPNLSTAENFLR"
BASE COUNT          345 a          299 c          323 g          410 t
ORIGIN      
        1 atttctcccg ctccttcgca gtcggaagca gtaatgatcg gggtatgaac atagttaaat
       61 ccccgttcct ggaaaaacac atgaatcgca tatgccatct ggcttctcat gcgggctaca
      121 gctccaaagg tattggtcct ggggcgtaaa tgggcaatat cgcgcaggaa ctctaatgag
      181 tgatttttct tttgaagggg ataggtttct acgggcgcct ctcctaacac agttatcgcc
      241 aagccctgaa tctctacagc ctggcccgaa gcgggcgagg gaataagtaa acccgttgcc
      301 tcaatggagg cgccggtgct gattcgcttc aattgggctt ctgtttcctc ggtaatgccc
      361 cgatctcgat cgagcgttac ctggattgac gcaaaacaag atccgtcatt tacctgtata
      421 aaaacaagat ttttggtttc gcgcttggtg cgtacccagc catatacggt tacttcccgg
      481 ccgtcaggag cggaaacaag gatatccttg ataagctgtg ttttcatgga agcacactat
      541 cataattatt cagtatcagt caaagggcct taccgggtta tgcggtattt ttatacattt
      601 ttaagtatat ttatacaaaa atgatgtgta tctatgcatt tttttgattt tcaataggtt
      661 ttggacttgc gctaattcgt caatgcgtgt ataaaaatac atatttcttt accggagggt
      721 aatattatgc aatatgaagg tgcgtccgct tcgtactttg agtcactgtc ccggttaacc
      781 gagcaaaatg attgtattga cccggaactc tatgtcaaat acgacgttaa acgtggtttg
      841 cgtgattccg acggtaaagg ggttctcgtc ggcttaacca gaattggaaa tgtgtacggt
      901 tacgaggtgg tagatggaaa gcccgttgca gtacccggaa aattgattta tcggggatat
      961 gatgttgagg atattattaa cgacatcgaa aaaagcgagc aattcgggtt tgaacaggtt
     1021 atttatcttc ttctttttgg agagcttccc aatgcctccc agcttgccga gtttgtggct
     1081 ctgttgggtg aatttcgcga gttaccccag aactttgtag aggatatgat ccttaagtct
     1141 ccttcctcag atattatgaa caagctcgcc cgctcggttc ttgtctccta ttcgtacgat
     1201 cccaatcccg aggatgtgtc tatcgcgaat aacctttatc agtgtattca gctcatcgcc
     1261 cggttcccgg caatggttgc ctatgggtat caggcaaaaa gacgacacta tgacggtaag
     1321 agtatgtatc tgcataatcc attgcccaat ctgtccactg cagagaattt tttacgg
//
LOCUS       DAOQXQ010000552         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_123421_length_1369_cov_3.390411, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000552 DAOQXQ010000000
VERSION     DAOQXQ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123421_length_1369_cov_3.390411"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1369)
                     /locus_tag="PKH81_08500"
     CDS             complement(<1..>1369)
                     /locus_tag="PKH81_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ98116.1"
                     /translation="IFYVLITAKTVDKGGSIRTEGHYVEALYHAQAKIKENEQKSVFY
                     NSGVPFHSWETSRKAQREITLIATVSLVLAALLLLVVFRSPGPLFLTIGAIICGTATA
                     FSVTQMIFGSVHLLTLAFGTSLIGISMDYALHFFVSLKRSPLVHVCTAGLLGLITTLT
                     GYCVLAVAPFSLLRQISVFSIIGLLSVFTTVFLVFPRVSSKIKPANLPLYPVRVYLSL
                     VNQTARVSRRTRLVCICVLFLACIPGLLRLNFTSDIRTLYTMGKDLYESERMTRSIMN
                     HGMNGWYYIIEADSDEELLLKEEALSVYLDKEIDTQSITSYVATSRYLPSLARQKESW
                     TVIGDQLLPLCEEQIQSIGYEKKDVEVCIQEWNLAEENFLTPSVFFGLPVARFVSSLW
                     VGELEGKYYSAVFPLNAVSPEPLQQIANKIDGVFFVNKTADISAALERMSLGRLWLLM
                     ASFIV"
BASE COUNT          454 a          288 c          272 g          355 t
ORIGIN      
        1 attactataa aagaagccat aaggagccat aaacgaccta aagacattct ttctaacgca
       61 gcgctaatat ccgctgtttt gttgacaaaa aaaactccat ctattttatt tgcaatctgt
      121 tgtaacggtt caggactaac cgcattcaaa ggaaacaccg cgctataata ttttccttcc
      181 aactcaccca cccacagaga agatacaaat ctagctactg gtagaccaaa aaacacagaa
      241 ggagtaagaa aattctcttc tgccaagttc cattcctgaa tacatacttc tacatctttc
      301 ttctcgtatc cgattgactg tatctgttcc tcgcaaagag ggagcaactg gtcgccaatt
      361 acagtccacg attctttttg cctcgctaag gaaggaagat atcgtgatgt tgccacataa
      421 gatgtaatag attgtgtgtc aatttcttta tcaagatata cagatagtgc ttcttcttta
      481 agaagaagct cctcatctga atctgcttcg attatataat accagccatt catcccatgg
      541 ttcattatgg agcgagtcat ccgttctgat tcatacaaat cctttcccat tgtgtataaa
      601 gtccgaatgt cactggtaaa attaagccgc aacaacccgg gaatgcatgc aagaaaaaga
      661 acacagatgc atacaagtct tgtccttcgg gacacacgag ctgtttgatt aacaagagac
      721 agatagacac gaacaggata taagggaaga ttcgctggtt taatcttgct tgatacacgg
      781 gggaatacca agaagaccgt ggtgaataca gaaagaagcc caatgataga aaacacagat
      841 atctgtcgga gcagggagaa tggggcaaca gcaaggacac aatagccggt taatgttgta
      901 atcaggccaa gaagacctgc cgtacataca tgaacaaggg ggctccgctt taatgataca
      961 aaaaaatgaa gggcgtaatc catactaata ccgataaggc tggtgccaaa ggccaatgta
     1021 agcaagtgaa ctgaaccaaa aatcatctgt gttaccgaga aagctgttgc agtgccacag
     1081 atgattgcgc caattgtcaa aaataagggg ccaggagatc gaaaaacaac aagcaataga
     1141 agagccgcaa ggactaagga gacagtcgca ataagagtta tttccctttg agcttttctt
     1201 gaagtttccc atgaatgaaa gggcactcct gaattataaa aaactgattt ttgctcgttt
     1261 tcttttattt ttgcctgagc atgatacaag gcttctacat aatgtccttc agtgcgtatt
     1321 gatccgccct tatccacagt ctttgcagta ataagaacat agaatattc
//
LOCUS       DAOQXQ010000553         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_123424_length_1369_cov_3.247336, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000553 DAOQXQ010000000
VERSION     DAOQXQ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123424_length_1369_cov_3.247336"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKH81_08505"
     CDS             complement(<1..954)
                     /locus_tag="PKH81_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNQ98117.1"
                     /translation="MSSVSSSPAHLHSLRDPHKEAQRFIDAAVPHRIIRVCVVTEPGE
                     SYVAKVLRQRFPGILLIALRYQNEFFRDTDTLWDAVWRPASGISIGVFLSTHIDEEIL
                     PLTVCIPWKASENAWPERARQVWDEIAVFVRLQAQISKTRHYFGPRWLNNMVKNAILV
                     RHPTIFTKTRVPVCIAASGPSLCNVATLLKESSFPIIAVSSALSCLQDQHIIPDAVIS
                     SDGGYWALSHLTGLSEQIPMLAPLEASLPSKVLEHNPLAFLDFGSPLEHSLLGIFGYK
                     AHRTTACATVTGVAAQWALDISTGYVYSGGLDLAPSQSFSHC"
     gene            complement(954..>1369)
                     /locus_tag="PKH81_08510"
     CDS             complement(954..>1369)
                     /locus_tag="PKH81_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98118.1"
                     /translation="LAQQGPINQDKEVLLSRVHASLADGKIANLFSLSFSNLFDSLEP
                     IESDERSFTLYLAMIHRLKHLVGPANCMYTGQDKIVRIVVFSSKQLDHRMYLPQIMDP
                     MESLFAKHRLSSVTLSLFPSTSHKQDILSFLFGET"
BASE COUNT          431 a          282 c          307 g          349 t
ORIGIN      
        1 acaatgagaa aaggattgag acggtgctaa gtcaagtcct cctgaataca catatccagt
       61 tgaaatatcc aaagcccatt gagcggcaac accagttact gttgcgcagg cagtcgttct
      121 gtgagccttg tagccaaaaa tgcctaataa agaatgttcc aggggacttc caaaatcaag
      181 aaaggccagg ggattatgtt caagaacctt actaggcaaa gatgcttcta agggggcgag
      241 catcggtatt tgttctgata atccagttag atgggagagg gcccaatacc ccccatctga
      301 tgagataacc gcatcaggaa taatatgttg atcctgtaaa caagagagcg cagaagagac
      361 agcgataata gggaaagagc tctcctttag caaggttgct acattacata aactaggacc
      421 ggaagcagca atacaaacag gcactcgtgt ttttgtaaaa atcgttggat ggcgtaccag
      481 aattgcgttc ttaaccatat tgttgagcca tcttggacca aaataatggc gagtcttact
      541 aatttgtgcc tggagacgaa caaatacggc tatctcatcc caaacctgtc gggctcgctc
      601 aggccaggca ttctctgatg ccttccatgg aatacatact gttaagggga gaatctcttc
      661 atcaatatgc gtagataaaa aaacaccgat tgatatcccg ctcgccggtc tccaaaccgc
      721 atcccataag gtatctgtat ctctaaagaa ctcattttga tagcgaaggg ctatcaataa
      781 aatacccgga aaccgctggc gtaaaacttt tgcaacatat gactctcccg gctctgttac
      841 tacgcaaacc ctgattatcc gatgaggaac cgctgcatca ataaaacgtt gtgcttcctt
      901 gtgaggatca cgtaaagaat gaagatgcgc aggcgaagaa gaaacagaag acattatgtc
      961 tctccgaaga gaaaggatag tatatcttgt ttatgtgaag ttgaagggaa cagcgaaagg
     1021 gtaaccgagg ataaccgatg cttggcaaaa aggctttcca tgggatccat aatctgtgga
     1081 agatacattc ggtgatcaag ttgttttgag ctaaatacta caattcgcac tattttgtcc
     1141 tggcctgtat acatacagtt agcaggtcca acaaggtgtt ttaaccgatg tatcatcgcc
     1201 aaataaagtg taaaactgcg ttcatcgctt tctattggct ctaatgagtc aaagagatta
     1261 gaaaaagaaa gagagaataa atttgcgatt tttccatccg caagtgaagc atgtactcgg
     1321 gaaagtaata cctctttatc ttgatttatt ggtccttgct gggctaatg
//
LOCUS       DAOQXQ010000554         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_123427_length_1369_cov_3.211568, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000554 DAOQXQ010000000
VERSION     DAOQXQ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123427_length_1369_cov_3.211568"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKH81_08515"
     CDS             <1..967
                     /locus_tag="PKH81_08515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNQ98119.1"
                     /translation="ISGSGPATAATMATVALPEMKKYRYSDELASGTVAAGGSLGMLI
                     PPSVVFIVYALMTEQSIGKLFIAGIVPGIVISVLFCLSIFFRCLKNPSLGPVGPRASW
                     KERCICLAGVGETLLLFGFVMGGMFAGLFTPIEAAAVGAAGSIVIALAKRALTMRIFY
                     RILLETMRTAGMIFFIVASAVVFGRFLAVSQIPFTLASWFTNLPVPSFVTIALIILLY
                     LFLGCFIDSLALVLLTTPIFYPVIIALGCNPIWFGVLIVVITQMGVITPPVGVNVYVV
                     SGIERDIPLRTIFKGSLPFLYMLILFATLLIIFPDIALFLPNLGQ"
     gene            977..>1369
                     /locus_tag="PKH81_08520"
     CDS             977..>1369
                     /locus_tag="PKH81_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ98120.1"
                     /translation="MATLDVFNRILVCADFSRSSLRAFEYALALCSANPEAQLCLFHA
                     IPEPDAQFWKTYLYEIDQIDDKAKADIDRKLQECYLDRAPKGLTINTRVDVGSAAERL
                     LEAVQAESIDLVVLGREDSGGPSTLFWGD"
BASE COUNT          262 a          305 c          345 g          457 t
ORIGIN      
        1 cattagcggc tctggaccgg ctaccgcagc aaccatggca accgttgccc ttccagagat
       61 gaagaagtat cgctactctg atgagcttgc cagcgggact gttgccgcgg gaggaagtct
      121 tggcatgctt attccgccaa gcgtggtatt tatcgtatac gcccttatga ctgagcaatc
      181 cattggcaag ttatttatag ccggtattgt ccctggtatc gtcatcagcg tgcttttttg
      241 tctttccatc tttttccgct gtcttaaaaa tccctccctt ggtccagttg gtccaagggc
      301 tagttggaag gagcgttgta tctgtcttgc tggcgttggc gagacgcttc ttttgtttgg
      361 ttttgtcatg gggggtatgt tcgcgggttt atttacacca attgaagctg ccgcagttgg
      421 ggctgccggt agtattgtga ttgcattggc aaagcgggcg ctaacgatgc gcatttttta
      481 ccggatactc ctggaaacaa tgcgaactgc gggaatgatc ttttttattg ttgcctctgc
      541 ggtagtcttt ggtcgctttc tggcagtctc tcaaataccc tttacgcttg catcatggtt
      601 taccaatctt ccggtgccgt catttgtaac gatcgccctg atcatccttt tgtatctgtt
      661 tcttggttgt tttatcgatt cacttgccct ggtgttgtta accacaccga ttttttatcc
      721 ggtgatcatt gctctaggat gtaatccaat ttggtttgga gttcttattg tagtgattac
      781 gcaaatgggt gttattactc cgcctgttgg ggtaaacgtg tatgtggtaa gcggcatcga
      841 acgggacatt cctttgcgta caatattcaa gggctcttta ccgtttttgt acatgcttat
      901 cctgtttgct actctgttga ttatctttcc ggatattgct ttgtttttgc cgaatcttgg
      961 tcagtaggag gtgctcatgg ctacgctcga cgtgttcaat cgtattctcg tttgtgctga
     1021 tttttcccgg tcgtctcttc gcgcttttga gtatgccctt gccctgtgtt cggcaaatcc
     1081 cgaagctcaa ctgtgtcttt tccatgcgat tcctgagccg gacgcgcagt tttggaaaac
     1141 ctatttatat gagatagacc aaattgacga caaggcaaag gccgacatag accggaagtt
     1201 gcaagagtgt tatctcgatc gcgctcctaa aggtcttacg ataaacaccc gagttgatgt
     1261 tggtagtgca gcagaacgtc ttttagaagc ggttcaagcg gaatcgattg atcttgtcgt
     1321 tctgggtagg gaagattccg gcggtccttc aactcttttt tggggggac
//
LOCUS       DAOQXQ010000555         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_123573_length_1368_cov_4.001523, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000555 DAOQXQ010000000
VERSION     DAOQXQ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123573_length_1368_cov_4.001523"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKH81_08525"
     CDS             complement(<1..976)
                     /locus_tag="PKH81_08525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ98121.1"
                     /translation="MVKLEAISEKTNFQEKFCYRQVGSATWQIDPVDVSSSLEYVMAQ
                     FNNSPDLPAIPVERNGALIGILERSHVEKMTESAWARFWQKDLDAYITAPLLSLRADD
                     YIEKHIVRGMQLNNEQGIRYFAVYYRRSFFGIAGLHQMLERMTELRTQDLAKAYAVQR
                     HLLESNCAQPDPRFSLITWNRMANEVGGDFYKDFSLAGEDRHIIGCFDVSGKNVAASL
                     STMAIGSFFTALALCDTGSRSFGELTSLMDAYVQNLTPSDVFITAALCYVDTAKGSLF
                     IQNCGHTPVYLFVPGENRKIVGKTLPANLPPLGMGIVGGEEATGYRVPI"
     gene            complement(1029..>1368)
                     /locus_tag="PKH81_08530"
     CDS             complement(1029..>1368)
                     /locus_tag="PKH81_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ98122.1"
                     /translation="LLNREGYVTECTGDNIFIVKKGVLKTSAPWLGLLEGITRNVVIS
                     LAKDLGIPVEETTMTRFDLYTADEVFLTGSGAEMIPVRCIDGRLIGTGSRGEITSRVM
                     EAFRLAVGAC"
BASE COUNT          419 a          349 c          261 g          339 t
ORIGIN      
        1 caattggaac ccgatagcct gtggcttctt cgccaccaac aattcccatg ccaagagggg
       61 gaagatttgc gggaagtgtt ttaccaacaa tctttcggtt ttctcccgga acaaacaggt
      121 acacaggcgt atgcccgcaa ttttgaataa acaaagaacc tttagctgta tccacatagc
      181 aaagggccgc ggtaataaag acatcgctcg gggttaaatt ttgaacatac gcatccatca
      241 acgaagtaag ctccccaaaa gaccgggaac cggtatcaca aagcgccaag gcagtaaaaa
      301 atgagccgat agccatggta gaaagcgaag ctgcgacatt ctttccggaa acatcaaaac
      361 aaccaataat atggcgatct tctccagcca ggctaaagtc cttgtaaaaa tctccgccaa
      421 cttcatttgc cattcgattc caggttataa gagaaaatcg tggatctggc tgtgcacaat
      481 tactttccaa taaatgtcgt tgtactgcat acgccttggc taaatcttga gtcctgagct
      541 ctgtcatccg ctcaagcatc tgatgcaaac cggctatccc aaaaaaagaa cgtcgataat
      601 aaacagcaaa atatcgaata ccttgctcat tattaagctg catccctcgc acaatgtgct
      661 tctcaatgta atcatcagcc cggagcgaaa gaagaggcgc tgtaatatac gcgtccaggt
      721 ctttttgcca aaaccgggcc caagccgatt cagtcatttt ttctacatga gagcgttcca
      781 gaattccaat aagggccccg ttacgctcta ccggtattgc cggcaagtcg ggtgagttat
      841 taaactgagc catgacatac tccagagacg aactaacgtc cactggatca atttgccagg
      901 tagcagaacc aacttgtcga taacaaaatt tttcctggaa attggttttt tcagaaattg
      961 cttctaactt aaccatggct tagtatcagc ccgtttacca agtcagtcaa cgacaaaaaa
     1021 atgatctttc aacacgctcc aactgctagc cgaaaggctt ccattacacg agaagttatc
     1081 tctcctctgg agccagttcc aataagcctg ccgtctatac accgaacagg tatcatttcc
     1141 gcgcctgacc cggttaaaaa gacctcatct gcggtataca aatcaaaacg tgtcatcgtt
     1201 gtttcttcaa caggaatacc caagtccttg gctaacgaaa taaccacatt acgagttatc
     1261 ccctcaagta aacccaacca gggagcgcta gttttaagaa ctcccttttt tacaataaaa
     1321 atattgtcac ctgtacactc cgtaacgtag ccctctctat ttaagaga
//
LOCUS       DAOQXQ010000556         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_123756_length_1367_cov_2.641768, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000556 DAOQXQ010000000
VERSION     DAOQXQ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123756_length_1367_cov_2.641768"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..>1367)
                     /locus_tag="PKH81_08535"
     CDS             complement(190..>1367)
                     /locus_tag="PKH81_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ98123.1"
                     /translation="KKAGSDVMMALNLGTRGIEDARNFVEYCNHPGGLYWSDLRRSHG
                     REEPYNFRVWCLGNEMDGPWQIGCKTADEYGKLAGEVAKALKLFDPDLELVVCGSSGP
                     GMPTFPQWEATVLEYCYDHVDYISLHTYARKEGDDSYTFLFESVKFENFIKTVESTVQ
                     FIKAKKRSRKDVKLSVDEWNVWYHSNGQESDDDLWQVGRRLLEDVYTVEDAIVVGCFL
                     IVLLRHAHSVRMACLAQLVNTIAPILAEPEGPAWKQTIYYPFLHCSLYGRGEVLETRI
                     ESPLFESDVYEPVPYVDSVAIVAHEDKQISFFCVNRHLSDNVEVSVRLMGFSGLSVIE
                     HITVCDSDPLARNTRDDPQRIVPKIKATTVCTGDCLTMMLSPLSWNVIRVSYRDRAE"
BASE COUNT          405 a          359 c          257 g          346 t
ORIGIN      
        1 caaaggccca agggttcttc ctccaatgat aaatccttca ttcccgccag tgtgcttctt
       61 cattgaatac acaccaatcg cgagtagaac cacgagatac accaaaaact ctattatgta
      121 aatcatttca aaatactacg tctagagaga aaggctgtca agaaaaagaa ctgagcattc
      181 cacaatagat cactctgcgc gatcgcgata ggaaaccctg atcacattcc aggaaagagg
      241 agagagcatc atggtaaggc aatccccagt acacacggtt gtcgccttaa ttttaggtac
      301 aattcgctgt ggatcatccc gggtatttcg cgcaagcggg tcagaatcac agacagtaat
      361 atgttcaatt actgaaagac cggaaaaacc cataagacgc acggacactt ccacattatc
      421 ggaaagatgt cggtttacac aaaaaaagct tatctgctta tcctcatgtg caacgatggc
      481 taccgaatca acatacggaa caggctcata cacatcgctt tcaaacaggg gagattctat
      541 gcgcgtttcc agcacctcgc cccttccata gagtgaacaa tgtaaaaagg gataatagat
      601 tgtttgcttc catgccgggc cttctggttc tgcaagtatc ggggcaatgg tatttaccag
      661 ctgcgcaaga caagccattc taaccgagtg agcatgccga agcagcacga taaggaaaca
      721 accaaccact atcgcatctt ctacggtata tacatcctca agtaaccgtc tgccaacttg
      781 ccaaagatca tcatcacttt cttgtccatt ggaatggtac catacgttcc actcatccac
      841 cgaaagtttt acatctttac ggcttcgctt cttcgccttt ataaattgca cagtgctttc
      901 tactgtttta ataaaatttt caaacttaac agattcaaat aaaaaagtat aactgtcatc
      961 cccttcctta cgcgcatagg tatgcaagga gatataatca acatgatcgt agcaatattc
     1021 caaaacggtc gcttcccatt gtggaaaggt cggcattccc ggtccagaac ttccgcaaac
     1081 aaccagctca aggtcaggat caaaaagctt aagagccttt gccacctctc ctgcaagctt
     1141 gccatactca tcggcagttt tacaaccgat ttgccatggt ccgtccattt cgtttccaag
     1201 acaccacacc ctgaagttat acggttcttc tctaccatgc gagcggcgta aatcgctcca
     1261 gtacaggcct cctggatgat tgcaatactc tacaaaattt cgcgcgtcct caatacctcg
     1321 agtcccaagg ttaagggcca tcatcacatc gcttccggct ttctttg
//
LOCUS       DAOQXQ010000557         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_125024_length_1359_cov_2.019939, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000557 DAOQXQ010000000
VERSION     DAOQXQ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125024_length_1359_cov_2.019939"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKH81_08540"
     CDS             <1..415
                     /locus_tag="PKH81_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ98124.1"
                     /translation="ESSDFCLEITETATRLSPALMNENMAKFVAAGFRLAIDDYGTGY
                     SNVEMMLETTFHTVKIDRSMITAADQSEEGRKVLEDLIMMFKSMDLSIVAEGVETGDQ
                     LAMVESLGVDLIQGYYYSRPLPEKEFVLFLDKRRR"
     gene            complement(1168..1296)
                     /locus_tag="PKH81_08545"
     CDS             complement(1168..1296)
                     /locus_tag="PKH81_08545"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98125.1"
                     /translation="MIDFTDRNTCVLFGDGAGAIVLGPAGHEDEGIFDVLIGADIA"
BASE COUNT          338 a          344 c          276 g          401 t
ORIGIN      
        1 ggaaagttca gatttttgtc tggagataac cgaaacggcc acgcgactct ctccggcctt
       61 gatgaacgag aacatggcca aatttgttgc agctggattc cgtcttgcca ttgacgatta
      121 tggaaccggc tattcgaatg ttgaaatgat gttggagacc acctttcata cagtaaaaat
      181 tgaccgttca atgataaccg ctgccgacca atccgaggaa gggagaaagg ttctggaaga
      241 cttgatcatg atgtttaaga gcatggatct ttccattgtt gcggagggag tagaaacagg
      301 cgaccagcta gccatggttg aatcccttgg cgttgacctg attcagggat actattactc
      361 ccgccccctg ccggaaaaag aatttgtttt attcctggac aagaggaggc ggtaatgctt
      421 gtaatggatt atgacgttgt tgccgtcttg ataaatacca tgacgctctc tctgttctat
      481 gcaaagaaga gggtgtccta ccaacataat tttttgtttg tgactattct gtggctattg
      541 ctgtccggct ctctttttaa tatacccagt gtgtatggtc ttaataatac ccagcaagtg
      601 ccacttatcc ttaccctgac ctttttacat atagccttta tcatctttca taccctgccc
      661 ttcttgactg ccctgtatat ttcttcccgc accaggctca ccctgcaaaa cagctcgacc
      721 gtgcgcctta ccgcccttcc ctgggtatct gcgatgctcc ttattgcatc aaacccgtta
      781 accggttggg tatataccct cactgagcgg caaggctatc ttcgagggcc actgtatcct
      841 gctttgtatc ttcccatact tttttatgtt gcgctcatag ctcgcgcaat aatgcgtcca
      901 accagaaagg ttaacaaaag agatggtaag gcgtttatga ttgccctcct gcttcccgta
      961 atcgcgatca tacttaaacc cttctttcct catgtcttgt tggagtcatt tgcaacatct
     1021 ctggtaggcc tttttattct gttaactatc caaaacagcg cggaccttat tgatggcaca
     1081 acagggcttt tttcaaaatc tgcctttacc cgtctcctcc aggagagaat cgacgaaaaa
     1141 tcgcgtttta ctcttttagc tattcattta agcgatgtcc gcgcctatca aaacatcgaa
     1201 aatgccttca tcttcatgcc cggcgggacc caatacaata gctcccgctc cgtcgccaaa
     1261 aagcacgcag gtatttctat cggtaaaatc tatcacttta gataatatat ccgcccctat
     1321 aaccaggaca tattcacaaa cgccggtttc aataagcga
//
LOCUS       DAOQXQ010000558         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_125464_length_1356_cov_2.593390, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000558 DAOQXQ010000000
VERSION     DAOQXQ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125464_length_1356_cov_2.593390"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKH81_08550"
     CDS             <1..360
                     /locus_tag="PKH81_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ98126.1"
                     /translation="IASDHAPHSLEDKQKGAPGFSGIQAVFPVCYTALVSNKRISLSR
                     LSALLSANPASILGIQAGLIGPGYPADLVVVNTEGETLFEGQSWYSKSINSPYLGHRY
                     AGQVVATFYQGKMVFAR"
     assembly_gap    433..442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <443..787
                     /locus_tag="PKH81_08555"
     CDS             <443..787
                     /locus_tag="PKH81_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ98127.1"
                     /translation="RQIIRSIESAILSGFLKKGEKLPTIRSLAIELKINPNTIAKAYA
                     ELEQRGTVTTQVGSGTYVSAQMPGEGVPERQELINDVLRCCIRDLKALGVSIQEIPGM
                     IEQFKEAIDETV"
     gene            774..>1356
                     /locus_tag="PKH81_08560"
     CDS             774..>1356
                     /locus_tag="PKH81_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ98128.1"
                     /translation="MKQYKDTIPEILVKNPVKRRSPLVIFGIRSSIVIGFVVIGVILR
                     FVMGYTMVSEQGLLWTVIPVVFGLSIVLSVRKMNEWERAIIFRFGRFSRVKGPGLFLL
                     MPLAEQIASIVDLRIRVSDFTTQNTLTRDSVTVTVDALCFWLVWDPEKAICEVQDYEA
                     AVILSAKTALRSAVSSHDLTLFLEKGDVIEQYIR"
BASE COUNT          362 a          258 c          331 g          395 t
ORIGIN      
        1 attgcttcgg atcatgcccc tcatagcttg gaagacaaac agaagggagc tcccggtttt
       61 tccgggattc aggccgtatt tccggtctgt tatacagccc tcgtatcaaa taagcgtatc
      121 agcttatcac gactttcggc gcttctttcg gcaaatcctg catcaatcct tggaatacaa
      181 gccggattaa taggtcctgg atatccagct gatctggttg tagtcaacac tgagggtgaa
      241 accctctttg agggacagag ctggtattca aaaagcatta attctcccta tctcgggcat
      301 cggtatgcag gccaagtcgt agcgaccttt tatcagggaa aaatggtttt tgcacgataa
      361 ttttcttttc tcttgacaaa ttgtactagt acaaactagg atttgtacta gatgagtagt
      421 acaattatga atnnnnnnnn nncgccaaat tattagatct attgagagcg caattctttc
      481 cgggtttctc aaaaagggag aaaaactacc aacaatacgc tcgttggcaa ttgagctcaa
      541 gattaatcca aatacaatag ctaaagcgta tgcggagctg gaacagcgtg gaacggttac
      601 cacccaggtt ggaagtggta cctatgtatc tgctcaaatg ccgggagagg gtgtaccgga
      661 aagacaagaa ttgattaatg acgtattgcg ttgctgtatt cgggatctga aggctcttgg
      721 ggtcagtatt caagaaatac ctgggatgat tgaacagttt aaggaggcaa ttgatgaaac
      781 agtataagga tacaataccg gaaatccttg taaagaatcc ggtaaaaaga cgatctcctc
      841 ttgttatttt tggtattcgc tcatcaatcg ttataggttt tgtggtaatc ggggtaatcc
      901 tgcgctttgt tatggggtat accatggtaa gtgagcaggg ccttctgtgg acagttattc
      961 cagtggtttt tgggttgtct atagtgttat ctgttcgcaa gatgaacgag tgggagagag
     1021 cgattatttt tcgctttggg agatttagca gggtaaaagg tcccgggctt tttttactga
     1081 tgccccttgc cgaacagatt gcctcgatcg tggatttacg aattcgggtc agcgatttta
     1141 caacacagaa taccctaacc cgagattcag tgaccgttac cgtcgatgcc ctttgctttt
     1201 ggcttgtatg ggatcctgaa aaggcgatat gcgaagtcca ggattatgaa gcggcagtta
     1261 ttttgtctgc taaaaccgcc ttgagaagcg cggtaagcag tcatgattta acgctttttc
     1321 ttgaaaaagg tgacgtgatt gaacagtata ttcgta
//
LOCUS       DAOQXQ010000559         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_125859_length_1353_cov_5.134823, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000559 DAOQXQ010000000
VERSION     DAOQXQ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125859_length_1353_cov_5.134823"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PKH81_08565"
     CDS             <1..799
                     /locus_tag="PKH81_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98129.1"
                     /translation="QKIIHGGGGVWGDHVMAAHPQITVPQATSMVQYIMSLADAKPTV
                     KEIPLKGTFKMQAPANDNKRGGYLLRVAYQDKGTADMQPLTSEKIIALRNPVLDPELN
                     DVSHGTQLFTTPSRSFSMIGDNAYLGYKNIDLTEIKQIELLVQAQPRSGALGGIVEVH
                     LDSPDGTLVGKSEPIVPKEVDFRTAIMEMRKNSKGPNQGGELSAATIDFNALRRFMSI
                     SAKIPVNNLQGSHDVYFVFKNPEAGQDKVLVQMVEIQFQNKIETSGK"
     gene            827..>1353
                     /locus_tag="PKH81_08570"
     CDS             827..>1353
                     /locus_tag="PKH81_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98130.1"
                     /translation="MKKTTSRRQFLGAASTLIAGSMIASHDTFGIPNFINNLGKSGST
                     IKGVKLGVITYSFRDLPDQSAEATLEYIRQCGITNVEMMGGPAESFAGAPKNPVDFRV
                     LMPIWRKRQQQQELTEEDKKTLADVEAKNKAYREEMGAWRQKASLSKFEEFGKMYKKA
                     GIQIYAFKPDAFGMQ"
BASE COUNT          414 a          324 c          318 g          297 t
ORIGIN      
        1 tcagaaaatc attcacggtg gcgggggtgt ttggggcgat catgtaatgg cagctcaccc
       61 gcaaattacg gtaccgcaag ctaccagcat ggtgcagtat attatgagtc ttgccgatgc
      121 aaaacctacc gtaaaggaaa ttccgcttaa aggtactttt aagatgcagg ctcctgccaa
      181 cgacaacaaa cgcggtggct acctgctgcg cgtggcatac caagacaaag gcacagccga
      241 tatgcaacca cttacttcgg aaaagattat tgcgctgcgt aaccccgtgc tggatcctga
      301 gcttaacgat gtgtcgcatg gcacccaact gtttaccaca cccagtcgtt cgttctctat
      361 gattggcgat aatgcgtacc tcggctacaa gaacatcgac ttaacggaga ttaaacagat
      421 tgaactttta gtacaggccc aaccacggtc gggtgcattg ggcggtattg ttgaagtaca
      481 cctggatagc ccagatggaa cactggtagg caaatctgaa ccaatcgttc cgaaggaagt
      541 ggacttccgc accgccatca tggaaatgcg gaagaatagc aaaggaccaa accagggtgg
      601 cgaactgagt gctgccacca tcgactttaa tgcactgcgc aggttcatgt caatctcggc
      661 aaaaatacct gtcaataacc tgcaaggaag ccacgatgtc tatttcgtat tcaagaatcc
      721 ggaagcagga caagacaaag tgcttgtgca gatggttgaa atacaattcc aaaacaaaat
      781 tgaaacttcg ggcaaataac ttatccactc cataattaaa ttcactatga aaaaaacaac
      841 atccagacga cagtttcttg gagcagcctc aacgctgatt gcaggctcca tgattgccag
      901 ccacgatacc tttggcatac caaattttat caacaacctg ggcaagtccg gttcaaccat
      961 taaaggcgtg aaattgggag ttattaccta ctccttccgc gatctacccg atcaaagtgc
     1021 cgaagccacc ctggaataca tccgtcagtg cggcattacc aatgtggaga tgatgggcgg
     1081 tccggccgaa tcttttgcgg gcgctccgaa aaacccggtt gacttccgtg tcctgatgcc
     1141 tatctggcgt aaacgtcagc aacaacagga attaaccgaa gaggacaaga aaacccttgc
     1201 cgatgtagaa gcaaagaata aagcgtatcg cgaagagatg ggagcctgga ggcaaaaggc
     1261 atctctatcg aaatttgaag agtttggaaa aatgtataag aaagcaggaa tacaaatcta
     1321 tgccttcaaa ccagatgcat tcggtatgca aaa
//
LOCUS       DAOQXQ010000560         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_126692_length_1348_cov_2.732405, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000560 DAOQXQ010000000
VERSION     DAOQXQ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126692_length_1348_cov_2.732405"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKH81_08575"
     CDS             complement(<1..484)
                     /locus_tag="PKH81_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ98131.1"
                     /translation="MGQYDWLADIWTVGTFTAFDTETTGLDPKSNRIVEIGGLRFDNL
                     GIAARFNSLINPGRPMPEEASRINGITDTMLAGQPTAAEVFPDFLRFAGNSVLIAHNA
                     PFDISFVNEELSRSGKPALTNKVVDTRILARELFPGLPKYTLQDLARHFGITAIEAHR
                     A"
     gene            complement(496..>1348)
                     /locus_tag="PKH81_08580"
     CDS             complement(496..>1348)
                     /locus_tag="PKH81_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNQ98132.1"
                     /translation="TDPDADRFGSAVPDKDGRFVLISGNQMGALLTDYICLSRTQTNT
                     MPSNPAIIRSIVTSQMGDRIAASYGVDSVECLTGFKWIASVMASFEEKGSHNYVFGYE
                     ESYGYNIETEVRDKDGISAAALCAEMTLYWRSQGKSLLDRLNELFTQHGYFEERAISK
                     AFPGASGGQTMKNLMSKLRNEGLARLGGIPVQSIRDIEQGIVYNPSSPTVRKPSGLPS
                     SNVLQFFLEDGTVVSARPSGTEPKIKFYISSPLPVGGKDLEEVKKAVSSKIALIEADI
                     RAVLGEI"
BASE COUNT          352 a          314 c          317 g          365 t
ORIGIN      
        1 cggcgcggtg agcttcaatg gcagtaatcc cgaaatgccg tgcaagatcc tgtaaggtgt
       61 attttggaag gcccgggaat aattctcggg caagaatacg tgtatcaaca actttgttag
      121 tcagtgcagg cttacctgag cgggagagct cttcgttaac aaaactgatg tcaaaaggtg
      181 cattgtgtgc aattagcact gaattgccgg caaaacggag aaagtcaggg aacacttcgg
      241 ctgctgtggg ctgaccggca agcatggtat cagtgatacc gttaatcctg gatgcttctt
      301 cgggcatggg gcgtcctgga ttgatcaagg aattaaaccg agctgcaatt ccaagattgt
      361 caaagcgtaa tccaccaatc tctacgatcc tgtttgattt aggatctagc cctgtggttt
      421 ccgtatcgaa ggccgtaaag gtccccactg tccatatatc tgccagccag tcatattgtc
      481 ccatgctcag tgtacctata tctcaccgag cacagctcga atatcagctt caataagcgc
      541 gatttttgat gataccgctt tttttacttc ctcaaggtct ttaccaccca cggggagggg
      601 agaggagatg taaaatttaa tctttggctc ggttccgctt ggacgggcgc ttacgaccgt
      661 tccatcctct aggaaaaact gaagaacgtt gcttgaaggc aaaccgctcg gttttcgtac
      721 agttggggag gaaggattat atacaatacc ttgttctatg tccctgatgc tttgtacggg
      781 aattccacca agacgagcca atccttcgtt tctgagttta ctcatcagat ttttcatagt
      841 ttgtccgcca ctagctccgg ggaatgcctt gctgatagcc ctttcttcaa aataaccatg
      901 ttgggtaaac agctcattca accggtcaag gaggctcttc ccctggcttc gccaatataa
      961 agtcatttcg gcacaaaggg cggcggcaga aataccgtct ttgtcccgaa cttcggtctc
     1021 aatattgtaa ccatagcttt cttcataccc aaaaacatag ttatgagagc ctttttcttc
     1081 aaaggaggcc attaccgatg cgatccactt aaagccagta aggcattcca cagaatcaac
     1141 tccataagaa gcggcaatac ggtcgcccat ttgagatgtt actatggatc tgataattgc
     1201 gggatttgaa ggcattgtat tagtttgtgt acgggaaagg caaatataat cggttaataa
     1261 agctcccatc tgattacccg aaataaggac aaatcgtccg tccttgtcgg gaacggcact
     1321 tccaaagcga tctgcatcgg ggtcagtt
//
LOCUS       DAOQXQ010000561         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_126720_length_1348_cov_2.415313, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000561 DAOQXQ010000000
VERSION     DAOQXQ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126720_length_1348_cov_2.415313"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKH81_08585"
     CDS             complement(<1..274)
                     /locus_tag="PKH81_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ98133.1"
                     /translation="MELELLGTGTSHGIPVISCTCDVCASVDSRDKRLRASALIRLDN
                     GMTILIDSGPEFRIQALRAGLSRLDTVLFTHSHADHTHGLDDVRIFT"
     gene            371..991
                     /locus_tag="PKH81_08590"
     CDS             371..991
                     /locus_tag="PKH81_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98134.1"
                     /translation="MAPREEERELMLVLDYILNRCTLREIDAVETAVERRRMDLTSQT
                     GIVSLDPSRAAQKMSGVVQDSIEKGMEGIRRTFREYAVDLIRKEAPELSEDQMAELID
                     GWIPENRSHDEKPATPGPYSGLSRKGLINGVPPDAMYEMVCQFVAYSTGKMGISEEAS
                     LRDEIGNWTDSYWKTFPAQIRECIRDFLSAAIDGAEMDRRLSRLLQ"
     gene            1001..>1348
                     /locus_tag="PKH81_08595"
     CDS             1001..>1348
                     /locus_tag="PKH81_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ98135.1"
                     /translation="MRIAVIGNGIAGINVAASLKTLGGDAVTVVVYSKEIHPFYSRVR
                     LPEVIAGESTPEDIRFYPEQWYEKKGFTVRLGTEVRSIDRAAKQLILVDGTAEHWDAL
                     VLATGARSNCPAIP"
BASE COUNT          331 a          303 c          390 g          324 t
ORIGIN      
        1 gagtaaagat gcgcacatca tcgagtccat gggtatgatc tgcatgagaa tgggtaaaaa
       61 gaactgtatc caatcgggat aacccggcgc gaagcgcttg gatgcggaat tcgggaccgc
      121 tgtcaatcag aatagtcatt ccattatcaa ggcgaatcaa ggcgcttgcc ctgagtcgct
      181 tgtcccggct gtcgactgat gcgcacacgt cgcaggtaca gctgattacc ggtataccat
      241 ggctggttcc ggtgccaaga agctcaagtt ccatgtttca gagcataaat ggagcgggca
      301 ggggcggtca agggctggcc cggcgctttg tcttgcgaaa tacggagtct cgggatattg
      361 ttataggtct atggcaccaa gagaagaaga gcgggaatta atgctcgttt tggattatat
      421 acttaatcgt tgtaccctgc gtgaaataga cgcggtagaa actgcggtag aacggcgcag
      481 gatggatttg acgagccaga cgggtattgt ctcccttgac ccttcccggg cagctcaaaa
      541 gatgagcggt gtggtacagg attcaattga gaaggggatg gagggaatac gacggacgtt
      601 cagggaatac gccgttgatt tgattcgcaa ggaagctcct gagcttagcg aggatcaaat
      661 ggccgagctg atcgacggct ggattccgga gaatcgcagt cacgacgaaa agcctgctac
      721 acccggtccg tattccggtc tttcccgcaa gggattgatt aacggcgtac ctccagacgc
      781 catgtacgaa atggtctgtc aatttgtggc ctatagcacc ggtaagatgg gcatatccga
      841 ggaagcctcg cttagggacg aaataggcaa ttggactgat tcctattgga aaacctttcc
      901 tgcccagatt cgcgaatgta ttcgggattt tttgtccgca gccatagacg gggccgagat
      961 ggacagaaga ctttccagat tattacagta ggagatctct atgcgtattg cggtaattgg
     1021 caacggcatc gctggcatca atgtggcggc ttctcttaaa accttgggcg gggatgcggt
     1081 tacggtcgtt gtgtatagta aagaaattca tcctttttat tcccgagtgc gccttcccga
     1141 ggttattgcg ggagaaagca cccccgagga tatccggttt tatcccgaac aatggtatga
     1201 gaaaaaaggt tttacagttc gccttggcac tgaagttcgc tcgattgaca gggcggcaaa
     1261 gcaacttata ttggtagatg gaacagccga gcactgggat gccctggttt tggccactgg
     1321 tgcccgctct aactgtccgg ctattccc
//
LOCUS       DAOQXQ010000562         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_127175_length_1345_cov_2.875194, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000562 DAOQXQ010000000
VERSION     DAOQXQ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127175_length_1345_cov_2.875194"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKH81_08600"
     CDS             <1..458
                     /locus_tag="PKH81_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98136.1"
                     /translation="SRSVTSRSAASPVYQSAPVAAPQATESAVKAPPPAAPAVPGPAK
                     MSSLVSVVVSNELFHNGNVEAWKRIIRSYNAKYPNLQVMVYYDGERIVDINTLFKWGK
                     VKHGSCIQFSVAGENIQDLAKLSRYFRQGASPQFEAFLHGSPDTVMNLF"
     gene            469..>1345
                     /locus_tag="PKH81_08605"
     CDS             469..>1345
                     /locus_tag="PKH81_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNQ98137.1"
                     /translation="MNSEKSIHYFSQKETVSKKIDKGMLGIRGRQANEFAELGLPILP
                     GFIIDSELASSLEGEQVYKLVSPYLRKCSDVVGKSFGDQDNPLLMKLVISPNLAIANY
                     PTLHNFGLTKNTIAGFEKWVGTNFAAHEVLFLLRGILSIQLQCAQLEERKIEVSALEV
                     ALKEIGECLKTGKIEGAGSSVMDRYAHFLPGGFFETPEQQIELSLKAISRLLALDEQN
                     DSDTAILVQPMVYGNYGKSSCSGSFFSRNIITGEKILQGKFFEEKFDDINAVGKDINQ
                     ITPAFLKKLQQIAWML"
BASE COUNT          368 a          283 c          309 g          385 t
ORIGIN      
        1 aatcccgttc tgttacttcc cgttctgctg cttctccggt gtatcagagc gcccctgttg
       61 cagctcctca agctaccgag tctgcagtta aagcgccgcc tcctgccgca ccggcagtac
      121 ctggccctgc caagatgtct tcgcttgtat ctgtagtagt aagcaatgag cttttccata
      181 acggaaatgt tgaagcctgg aagcgaatta ttcgcagcta caacgccaag taccccaatc
      241 ttcaggtcat ggtgtattat gatggcgagc gaatcgtgga tatcaatacc ttgtttaagt
      301 ggggtaaggt caagcatgga agttgtatac agttcagcgt agctggagaa aacatccagg
      361 atcttgccaa actttcccgt tatttccgtc agggagcaag tcctcagttt gaagccttcc
      421 ttcatggatc gcctgataca gttatgaatc ttttctagga gtgcatgtat gaacagtgaa
      481 aaatcaatac actatttcag ccagaaggaa acggtaagca aaaaaatcga taaagggatg
      541 ttgggtattc gtggtcgcca ggcaaatgag tttgctgaac ttggcttgcc aatccttccg
      601 ggttttatta tcgattcaga acttgcgtcg tccttggaag gggagcaggt atataaactt
      661 gtttcgccct atttacgtaa atgtagcgat gtcgttggaa aaagttttgg tgatcaagat
      721 aaccctcttt taatgaagct ggttatctct cctaatcttg caatcgcaaa ttatcctaca
      781 ttacataact ttggattaac aaaaaacacc atagctggtt ttgagaaatg ggtaggaaca
      841 aacttcgcgg cccatgaggt tctctttttg cttaggggta ttttgagtat tcaattgcaa
      901 tgtgcacagc tggaagagag aaagatcgaa gtatcagctc ttgaagttgc attaaaagaa
      961 attggtgagt gtttaaaaac cggaaagatc gaaggggcag gcagttctgt tatggatcgc
     1021 tatgcgcatt tccttcctgg tggattcttt gaaactcctg aacagcagat tgagctttct
     1081 cttaaggcaa taagtcggtt gcttgctctt gacgagcaaa acgacagtga taccgccatt
     1141 cttgttcaac ccatggtcta tggcaactat ggcaagagct cctgttctgg atcatttttt
     1201 agccgaaaca tcatcactgg agaaaaaatc cttcagggta agtttttcga agaaaagttt
     1261 gacgatatca atgccgtcgg taaggacata aatcagatta cccccgcatt cctcaaaaaa
     1321 ttacagcaga ttgcctggat gctcg
//
LOCUS       DAOQXQ010000563         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_127940_length_1341_cov_1.911353, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000563 DAOQXQ010000000
VERSION     DAOQXQ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127940_length_1341_cov_1.911353"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKH81_08610"
     CDS             complement(<1..432)
                     /locus_tag="PKH81_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNQ98138.1"
                     /translation="MAKQLLFNEEARKKLLSGVEQISRAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEIELEDSFENMGAQLLKEVATKTNDVAGDGTTTATVLAYSMVREG
                     LKSVAAGMTPIELKRGIDKAVIIAVEEIKKNPNSRQLYLNIW"
     gene            586..>1341
                     /locus_tag="PKH81_08615"
     CDS             586..>1341
                     /locus_tag="PKH81_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98139.1"
                     /translation="MNGTVVQWGIVATGGIAAKFAQACNFESNRSGKSIIAAVASRSL
                     DKALDFAQKWSIPRAYGSYEELFADPLITAVYIATPHSSHPELSIQALKAGKAVLCEK
                     PVAITLKDFLPVIQTARECKLFFMEAMWMKFNPCFQKMLSWIQEGRIGRVKYLRSDFF
                     LDAPFNPAGRYYNPALGGGALLDVGIYPVTCAVMVGGRRRPQSIDSWMRFAQTGVDLY
                     GKVRMVWDDALVADLASSIDLQGLDHMSSAVVIG"
BASE COUNT          301 a          296 c          331 g          413 t
ORIGIN      
        1 ccaaatgttg agatagagtt gcctactgtt ggggttcttc ttgatttctt caacagcaat
       61 gattactgct ttgtcgatac cgcgctttaa ctcaataggg gtcattcctg ctgcgacgga
      121 tttcagtcct tcgcgaacca ttgaataggc cagaacggtt gcagtggtgg ttccgtctcc
      181 ggctacatca ttggtctttg ttgctacttc ttttagtagc tgtgctccca tgttttcgaa
      241 ggaatcttca agctcgattt ccttcgcaac ggatacacca tccttggtta cggtcggggc
      301 tccgaatttc ttgtccaaaa gaacgtttcg tcccttgggt ccaagtgtaa ccttgacggc
      361 gcgggaaatc tgctccacac cggaaagaag ctttttccgg gcttcctcgt taaacagtag
      421 ctgtttggcc atttcttttc tccttattgc tctacctgaa tggtattaaa taaacgaaag
      481 taccccttct tttttggggg ttcaccgtat aaaatacaga aaactaccct aaacggcaat
      541 cggttttttt tcaatagggt taaagttagg gatacggagg ttgggatgaa tggtactgtt
      601 gtacaatggg gaatagtcgc tactggtgga attgcagcca aatttgcaca ggcgtgtaat
      661 tttgagtcaa atcggagcgg aaaaagcatt atcgctgcag ttgcttcgcg gtcgctcgat
      721 aaggccctcg attttgccca gaaatggtct attccgcgcg catacggctc ctatgaagag
      781 ctgtttgccg atccccttat tacggcggtc tatatagcaa ccccgcactc ttctcatcca
      841 gagctttcaa tacaggccct taaagccggg aaggctgttc tttgcgaaaa accggttgct
      901 atcaccttga aggatttttt accggttatt caaactgcac gggagtgcaa gcttttcttt
      961 atggaagcta tgtggatgaa attcaaccct tgttttcaaa agatgctttc ctggatacag
     1021 gaaggcagaa ttggacgggt aaaatatctt cgttcggact tttttttgga tgcaccgttt
     1081 aaccctgccg gacgctacta taaccctgct cttggcggag gagccctttt ggatgtaggt
     1141 atttatccgg ttacctgtgc ggttatggtt gggggaagga ggagacccca atctattgat
     1201 tcctggatgc gttttgcgca aaccggagtg gatctgtatg gtaaggttcg tatggtctgg
     1261 gatgatgctc ttgttgccga tcttgccagt tctatagatc tccaaggatt ggatcatatg
     1321 agcagtgccg tcgttatcgg t
//
LOCUS       DAOQXQ010000564         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_128304_length_1338_cov_2.780203, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000564 DAOQXQ010000000
VERSION     DAOQXQ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128304_length_1338_cov_2.780203"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKH81_08620"
     CDS             <1..197
                     /locus_tag="PKH81_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNQ98140.1"
                     /translation="FFSGFVYSMLGIPRELYTPIFAVSRIAGWAAHRLEEIVSGGRIY
                     RPAFKQVGDLREYIKLSERR"
     gene            complement(293..>1338)
                     /locus_tag="PKH81_08625"
     CDS             complement(293..>1338)
                     /locus_tag="PKH81_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ98141.1"
                     /translation="YRSIGKKLVETKELFAGAIGVATKLPIAKTNDTLSATQDAQPFM
                     KLRTPNPVYSMAVAAKDKKDDDKLGELLVRACEEDMTLSFSFNAETKQSVLSGMGDLH
                     INIILNRIKNQTKIDILTSIPRIAYRETIQRKSQAEYTHKKQSGGHGQYARVVLAIDA
                     LERGEGYTFSNAVFGGAISKGYIPGVEKGVKEAMENGVLAGYPVVDVGVTVLDGKEHP
                     VDSSEMAFKIASRNAFKDAMRNAGPILLEPIMNLTVFVQSSYLGDIMSDLSGRRGHIL
                     GQSSPGNGIEEIRAQVPHHELLRYAIDLRSMTSGTGSFDITFDHYSPISGKIADEVIT
                     AAKEFIKQQAEDD"
BASE COUNT          302 a          318 c          304 g          414 t
ORIGIN      
        1 atttcttctc gggttttgta tactcgatgc ttggtattcc ccgtgagtta tacaccccga
       61 tattcgcggt ttcccgcatt gccgggtggg cagctcatcg gcttgaggag attgtgtccg
      121 gcggcagaat ttatcggcca gcctttaagc aagttggcga tttacgagaa tatattaagc
      181 tttcagagcg ccgctagaat agaacgagat aaaaaaaaga ccggaggatt tctccggtct
      241 ttttttttgt aatacctgtt taatgctcaa atatactttt tatactgttg gtctagtcat
      301 cctcggcctg ctgtttaata aactctttgg ctgctgtgat tacttcatcg gcaatctttc
      361 cggatatcgg gctgtaatga tcaaatgtta tatcaaagga tccggttcca ctggtcattg
      421 aacgtaaatc aattgcgtaa cgcaataatt cgtggtgggg tacctgcgcg cgaatttctt
      481 caattccatt ccctggactt gactgtccta gaatatgacc tcgtcgtccg gacaagtcgc
      541 tcatgatatc acccaaatag gatgattgga caaatacagt caagttcata atcggctcaa
      601 gcagtatggg tccggcgttg cgcatcgcat ccttaaaagc gttgcgggaa gcaatcttga
      661 acgccatttc ggaagagtcc accggatgtt ccttgccatc aagcacagtt accccaacgt
      721 cgactaccgg atacccggcc aatacgccat tttccatcgc ttccttgacg cctttttcta
      781 ctccagggat gtagcctttt gatattgcac caccaaaaac tgcattggaa aaggtgtatc
      841 cttctccccg ctccagggcg tcaattgcca ggacgacacg cgcatactgt ccgtgtcctc
      901 ctgattgttt tttatgcgtg tattctgcct ggctctttcg ctgaatagtt tcccgatatg
      961 caattcgcgg aattgaggta aggatgtcaa tttttgtttg attcttgatt cgattaagaa
     1021 taatgttgat gtgcaggtct cccattcccg aaagaacgct ttgttttgtc tccgcgttaa
     1081 acgaaaagga tagagtcatg tcttcttcac aggcgcgaac caggagctct ccgagcttgt
     1141 cgtcgtcttt tttatccttt gctgccacgg ccattgagta caccggattt ggcgtgcgaa
     1201 gcttcataaa gggttgggcg tcctgtgttg cgctaagagt gtcgttcgtt ttggctattg
     1261 gaagcttggt tgctacaccg atagctccgg caaataattc ctttgtctct accaattttt
     1321 ttccgatact ccggtaca
//
LOCUS       DAOQXQ010000565         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_128953_length_1334_cov_3.314308, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000565 DAOQXQ010000000
VERSION     DAOQXQ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128953_length_1334_cov_3.314308"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1232)
                     /locus_tag="PKH81_08630"
     CDS             complement(210..1232)
                     /locus_tag="PKH81_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNQ98142.1"
                     /translation="MKQRGLFDEDERLQKISALGDPLEKLDKVINWNIFRPTLTTALR
                     KEAKGPGGRPPYDYVMMFKILILQRLYNISDAQTEYQIQDRLSFMRFLGLTLKDAIPD
                     EKTIWYFREQLVKEKVSEKLFKKFLTELEKQNLISRQGSIIDATFVDVPKQRNTKEDN
                     DIIKNDKVPEDWDKPENKAKKSQKDMDARWAKKLDETHYGYKAHVKADSKSKLILKYT
                     VTPASVHDSQELANLVDTKDVAVYADSAYVGEPIEKCLQRNKIKNNICAKAFRNKPMT
                     KKQEKENRKISRTRSRIEHIFGFMTNSMNGICIRSIGFARAEFNIGLMNLTYNMSRYR
                     FLEAQG"
BASE COUNT          329 a          280 c          247 g          478 t
ORIGIN      
        1 ttacgagcga tttaccctgg gaaaaagaca acgatagctg caaagtatca taaaagggaa
       61 cctcgaaaaa ctactgtttc tcgtgtatct ccgtcatatt aaactcaagt cctgtaaaac
      121 tagagcattg aaaacgattt caggcccttt tattttttcc tgattaccga ttttttaatc
      181 ggcaatcaaa aaagggcaga ctgccccttt catccttgtg cctcaagaaa tcggtagcgg
      241 ctcatattgt aggttagatt cataaggcct atgttaaatt ctgctctagc aaatccaatg
      301 ctacgaatac aaataccatt cattgagttt gtcatgaatc caaatatgtg ttctattcgt
      361 gatcgagttc ttgatatctt cctgttctct ttttcctgtt tcttggtcat cggtttatta
      421 cgaaatgctt tggcgcaaat gttgttctta atcttgtttc tttgtaagca cttttctatc
      481 ggctcgccga catatgcact atcggcgtac acggcaacgt ccttggtgtc tacaagattt
      541 gctaattctt gactgtcatg aacgctagca ggggttacgg tatatttcag tattaacttg
      601 ctcttgctgt cggcttttac atgagccttg tatccataat gcgtttcatc cagtttcttt
      661 gcccaacggg catccatgtc cttttgtgat ttctttgctt tgttttctgg tttatcccaa
      721 tcctcgggaa ccttgtcatt cttgatgata tcattgtctt ctttagtatt tctttgtttc
      781 gggacatcaa caaaggttgc atcaataata cttccttgcc gagatatcag gttctgcttt
      841 tccaattcag ttaagaactt tttgaaaagc ttctcactga ctttttcctt gaccagctgc
      901 tcccggaaat accatatggt tttctcgtcc gggatagcat ctttcaaggt taagccaaga
      961 aatcgcataa agctcaaacg atcttgtatt tggtattcgg tttgcgcatc gctgatatta
     1021 tacagtcttt gcagaatgag aatcttgaac atcattacat aatcatacgg cggtcttccg
     1081 ccaggacctt tcgcttcttt ccgtaatgcc gttgtcaatg taggccggaa gatattccaa
     1141 ttaataactt tatccagttt ttcaagagga tcaccgagcg cgcttatctt ctgtaatcgt
     1201 tcatcttcgt caaaaagtcc gcgttgtttc atgaggttat tttatcacat ttttcgatag
     1261 tttttagagg tgcccataaa gcattttgtg ttctctactt attaaaggag ttgagtgttg
     1321 tttatagaaa atgg
//
LOCUS       DAOQXQ010000566         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_129172_length_1333_cov_2.675274, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000566 DAOQXQ010000000
VERSION     DAOQXQ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129172_length_1333_cov_2.675274"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKH81_08635"
     CDS             complement(<1..230)
                     /locus_tag="PKH81_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ98143.1"
                     /translation="MTRSREAIYALLTSEPYPLSASDAWRLLHGQFDQATVYRTLHYL
                     EGKGMADSFVLHCSEHGTERYYTIREDSHVHR"
     gene            complement(227..1093)
                     /locus_tag="PKH81_08640"
     CDS             complement(227..1093)
                     /locus_tag="PKH81_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ98144.1"
                     /translation="MTTIISALMDPTLSFLRNAVLAGILSSILFGILGAIVTVRRVAS
                     LAGAISHAVLGGIGMALYLSTTGIVPGLPPVAGALAFAVIAAIIIGLVSLKAKQREDT
                     VINAIWAIGMSLGVLFMARTPGYTDPSSYLFGNILLVSTRDLWLLAALDIIVLVITWR
                     FYPQIKLSSFDEEYARVRGINTHSIFFIILTITAIAIVLLQTFVGIVMVIAMLTLPAG
                     TAGFRAKSLWLLMGSAIGWALFFSLGGLAAGWHFDLPVGAMAVVIAGLVFLVFSAHRA
                     LTRKGNKVERKS"
     gene            complement(1090..>1333)
                     /locus_tag="PKH81_08645"
     CDS             complement(1090..>1333)
                     /locus_tag="PKH81_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98145.1"
                     /translation="GSTTVLIVTHDTAFVSSLTDTVLCLGDRAADGRKRGIVRHETIP
                     ATNAPALPCPRIIRAAQVLHESTIQSHCGCCGEDEK"
BASE COUNT          366 a          330 c          365 g          272 t
ORIGIN      
        1 tgtcggtgca catgtgaatc ctcgcgtatc gtgtaatacc gctctgttcc atgttccgaa
       61 caatggagaa caaaagagtc tgccattccc tttccttcaa ggtaatggag cgtacgatac
      121 acggttgctt gatcgaactg tccgtggagc aacctccagg catcggaggc ggaaagggga
      181 tagggttcgc ttgtaagtaa cgcatatatt gcttcgcgag atcgcgtcat gatttcctct
      241 ccaccttgtt gcctttgcgg gtgagtgctc gatgagccga gaaaacaaga aagactaatc
      301 ctgcaataac aacagccatc gcgccaacag gaagatcaaa atgccatcct gcggcaagtc
      361 ctcccaggga aaagaagagc gcccagccaa ttgctgagcc catgagaagc cacaggctct
      421 ttgccctgaa cccggctgtt cctgccggaa gggtgagcat ggcgataacc ataacgatac
      481 caacaaaggt ttgtaaaaga acaatcgcga tcgcggttat ggtaaggata ataaaaaaga
      541 tactatgtgt gttaatgccg cgcacacgcg cgtattcttc gtcgaacgag gatagcttga
      601 tctggggata aaaccgccag gtgattacaa gaacgataat atccagagcg gccagaagcc
      661 acaggtcccg ggtggacaca agcaagatat tcccgaatag atacgaggac ggatcggtgt
      721 agccgggagt acgcgccata aagagcacac caaggctcat gccaatcgcc catatggcgt
      781 taataacggt gtcttctcgt tgtttcgctt taagagaaac aagaccgatg atgatagctg
      841 caataaccgc aaaggcaagg gctccggcaa ccggaggtag tccgggtact atgcctgtgg
      901 tagacaggta gagcgccata ccaatgccac cgagtacagc gtgggaaatc gctccggcaa
      961 gacttgctac tcggcggacg gttacaatgg cgccaaggat gccaaagagg atagacgaaa
     1021 gaatacctgc aagcacggca ttgcgtaaaa aggaaagggt agggtccatc aaggcagaga
     1081 taatagtggt catttctcat cctccccgca acagccgcaa tgggattgaa tagtactttc
     1141 gtgcagtacc tgcgccgccc ttattatacg ggggcaaggc agcgcaggcg cattcgtcgc
     1201 cggtattgtc tcgtggcgca caattccccg ctttcgacca tcggctgcgc ggtcgccaag
     1261 acagagaacg gtgtcggtaa gggaggaaac aaaggctgtg tcgtgtgtta ctatcaagac
     1321 cgtagtgctt ccc
//
LOCUS       DAOQXQ010000567         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_129258_length_1333_cov_1.663537, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000567 DAOQXQ010000000
VERSION     DAOQXQ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129258_length_1333_cov_1.663537"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKH81_08650"
     CDS             <1..213
                     /locus_tag="PKH81_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98146.1"
                     /translation="VMKSIHLALIALVFSFLSLTLSAQEEPEQGSKTYATIDEAFIAR
                     MSGLFAAEDPKPCIALTSTIARLNSF"
     gene            168..>1333
                     /locus_tag="PKH81_08655"
     CDS             168..>1333
                     /locus_tag="PKH81_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98147.1"
                     /translation="MYSFNLYDSEVEFFLDGSWEASLEGALLITFDHTGSRMSLSPPI
                     FTQRVDLTTWLFIDKTWYFEAAFAEEFTRNTVAAGYVGDDESAIKHVRIGNASIVFPD
                     RYPFIVVGGGRAIQPGIMGTFSGEGWKADALVRYDSLKEKEMVLSGMNEVTDTLIPIE
                     NPVRGKWFALPDPVVSGSVEVYVEDENGKFRDDTSYAAGRRWRKLDSDEWRLEGIGGI
                     LTLNSATTKSVAIVYSGAWAGAGPALQSFIQDTRLWFESSGQSISSSIIPDPSLPDYQ
                     SELANKYITRIDGKEALLIFERGRFSPFNLASRYECEGTTLDVINSESETPYTGFDTT
                     LLEGSWAELFITPATWGERRETREAESRFPLANRFPGIYLRPGLDTTGIVIRSRG"
BASE COUNT          361 a          313 c          331 g          328 t
ORIGIN      
        1 gtgatgaaga gcatacacct tgcccttatc gctctagtat tctctttttt atccctgacc
       61 ctgagcgcac aggaagagcc tgaacaaggc agcaaaacat atgcaacaat tgatgaagcc
      121 tttattgccc gaatgagtgg cttattcgcg gccgaagatc ccaaaccttg tatagcttta
      181 acctctacga tagcgaggtt gaattctttt tagacggtag ctgggaagca agtctggagg
      241 gagcgctact aattaccttt gatcacaccg gttcacgcat gagcctttcc cctccaatat
      301 ttacccaacg tgtagattta acaacctggc tctttatcga taaaacctgg tacttcgaag
      361 cagcctttgc cgaagagttt acccgaaaca ccgttgctgc cggttatgtc ggggatgatg
      421 agagtgcaat taaacatgtg cgcataggaa atgccagtat cgtttttcct gaccgctatc
      481 cctttattgt cgttgggggc ggaagggcta ttcaaccggg tataatggga accttctcgg
      541 gcgaaggatg gaaagccgat gccctggttc gctacgatag ccttaaagaa aaagagatgg
      601 ttctatcggg tatgaacgag gtcaccgaca cccttatacc aattgaaaat ccggttcgcg
      661 gtaaatggtt cgcccttccc gatcctgttg tttcagggtc agtagaagta tatgtggaag
      721 atgagaacgg aaaattccga gacgacacaa gctatgctgc cggcagaaga tggagaaaac
      781 tcgatagcga cgaatggaga ctcgaaggaa tcgggggaat acttaccctt aacagtgcaa
      841 ccacaaaatc ggtagcaata gtgtattcag gggcatgggc aggagctgga cctgctttac
      901 aaagttttat tcaggataca cgtctatggt ttgaatcctc aggtcaaagt atctcttcat
      961 caattatacc agacccatcg cttccggatt atcaaagcga gcttgccaat aagtatataa
     1021 cgcggataga cgggaaagaa gccctgctta tttttgaacg aggtcgcttt tctcccttca
     1081 accttgcctc gcgctatgaa tgcgagggga caacgctgga tgttataaac agcgaatcag
     1141 aaactccgta tacaggattt gacaccacgt tattggaagg gtcctgggcc gagctcttta
     1201 ttacaccggc aacctggggc gaacggagag aaacgaggga agcagaaagc cgcttccctc
     1261 tggcaaaccg cttccccggc atctatcttc gtccgggcct tgatacaaca ggtatcgtca
     1321 tacggtccag ggg
//
LOCUS       DAOQXQ010000568         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_129326_length_1332_cov_2.836335, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000568 DAOQXQ010000000
VERSION     DAOQXQ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129326_length_1332_cov_2.836335"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKH81_08660"
     CDS             complement(<1..703)
                     /locus_tag="PKH81_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98148.1"
                     /translation="MFSFLKRLFIKKSDVVPDLDDIPQENWVCTFSSKTDDRFTGECG
                     DGYEAIVNREGVILTIFRNNCFAWITNPVYRYQDAVIEALIEFPDPIPRADSMAGSMA
                     AGIHFRCLNDSTFYSVMVSDGGMIRLDAVVNETPLPVLGWTETAMGNQDSQGDALLPY
                     HENPNVFSLRIIARGTSITLIVNDEWVAGCEDDTIQAAGRIAFAAQTWKERKDVSVYL
                     KAIGIDSRPMEIEALH"
     gene            complement(709..1119)
                     /locus_tag="PKH81_08665"
                     /pseudo
     CDS             complement(709..1119)
                     /locus_tag="PKH81_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
BASE COUNT          347 a          313 c          315 g          357 t
ORIGIN      
        1 agtgaagtgc ttctatctcc atcggtcgac tgtcaatccc gatagcttta aggtatacag
       61 atacatcctt gcgctctttc cacgtttgtg ccgcaaaggc tatacgccct gcagcctgaa
      121 tcgtatcatc ctcgcatccg gctacccatt catcatttac gataagtgta atactggttc
      181 cccgggcaat aatccttaga gagaatacat tgggattttc gtgatagggt aatagtgcat
      241 caccttgtga atcctggttt cccatcgcgg tctctgtcca accaaggaca ggcagcggtg
      301 tttcattaac aactgcatcc aggcgaatca ttccaccatc agagaccatt acgctgtaga
      361 atgttgaatc gttgaggcat ctaaagtgaa ttcctgcggc catggatcct gccatagagt
      421 cggctcttgg aatgggatca ggaaattcga taagggcttc gataacagca tcttgatagc
      481 ggtatacagg attggtgatc catgcaaaac aattgtttct aaagatggtg agtataacgc
      541 cttcgcggtt tacgattgcc tcgtatccat cgccacattc gcctgtaaat cgatcatcag
      601 ttttggacga aaaagtgcag acccaatttt cctgggggat atcgtcgaga tcgggaacaa
      661 catcagactt ttttatgaaa agacgtttca ggaatgagaa cataccgctc aaaggtatcc
      721 caaatcacgg tatctgtcta cgcaaaagtc cactgcagag cgcatccgga ctaaacggaa
      781 gagtgcttcc ggtttcgctc caggaaaggt tcccctaatg atattgtcac tcttatgttc
      841 ctcaaaatct tgtccaatat gagcgaatcg ctcttgatgt aatacgggtt cctgccatac
      901 ggaacgacaa ggtaagccaa aatgtgatga tatcgctgtg tacgtaaggg tgtctgtgtg
      961 atccatccaa tctgccagat gaaaggctgt gcagctttcc caggagcagc cgagcatcaa
     1021 gataagggca tcttttgtat agaggctacc cagcggggac tgtgggccga gtccttggcg
     1081 ccatgtatgg gatgacatca atttttttgc tccgtaacct tccgcgcaaa agagtaaaaa
     1141 ggatgattag aacgcttgat tccagctgtt tttctaaaaa gctccggcaa agcgcccata
     1201 gcgcgacaag tagtgtccct tttgttatac acaaacggta ctgcaaaggc ctgtgctgga
     1261 tctggggtat cgctgaatgt gggcataacc agggtggcat cctttttttt gcagcttatc
     1321 cgcaaggcct ct
//
LOCUS       DAOQXQ010000569         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_129552_length_1331_cov_2.202978, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000569 DAOQXQ010000000
VERSION     DAOQXQ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129552_length_1331_cov_2.202978"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKH81_08670"
     CDS             complement(<1..625)
                     /locus_tag="PKH81_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98149.1"
                     /translation="MEEEKKYKEIELRSEEVQEVMGRVPPWILRWGIAVLLGILLILL
                     CVSFFYRYPDVITAEVTITTVDPPVSIIARATGKLDKIYVENNSQVSADVPLAVIQNP
                     ARTEDILWLSSQVSEWITAGSDLLMARQRFGARPLQLGSVQGAYSLFLTSLQDYRDYK
                     SLNYYPQKIAFQQKQLSMQHQYYGGVVEQNKLTEIQSELARSMFQRDS"
     gene            complement(641..>1331)
                     /locus_tag="PKH81_08675"
     CDS             complement(641..>1331)
                     /locus_tag="PKH81_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98150.1"
                     /translation="RDYVLENINLTIPENKVTAIVGASGSGKTTLIKLLLGFYEPNKG
                     SIKLGETPLKHLNPHMWRSKTGSVMQDGFIFSDSIAGNIAAGQEMIDPERLRHAVTVA
                     NIRDFIDSLPLGYNTKIGMEGNGISQGQRQRMLIARAVYKNPEFIFLDEATNALDANN
                     EKEIMEHLNEFYKGKTVVVVAHRLSTVRDADKIVVLDGGKIVEEGTHQELTATKGKYY
                     QLVKNQLELGQ"
BASE COUNT          365 a          360 c          235 g          371 t
ORIGIN      
        1 ccgaatcacg ctgaaacatc gatcgggcca actcagactg aatttctgtc aatttattct
       61 gctccaccac tcctccgtaa tactggtgct gcatactaag ttgcttttgc tgaaaggcaa
      121 tcttttgagg atagtaattt aacgatttat aatccctgta atcctgcaag ctggttagaa
      181 aaagagaata agctccctga acacttccca gctgaagagg acgagcgcca aaacgctggc
      241 gagccattag cagatcactt cctgctgtaa tccattcact aacctgcgaa gacaaccaaa
      301 gtatatcttc cgtacgtgcc ggattctgga taaccgccaa aggaacatca gccgacacct
      361 gactattatt ctctacataa atcttgtcca gtttacctgt agcacgagcg ataatactta
      421 ccggaggatc tacagtcgtt atggtaacct ctgcagtaat gacatccgga taacgataaa
      481 agaaacttac gcaaagcaag ataagcagaa tacccaataa tacagcaata ccccagcgca
      541 atatccacgg cggaactcgc cccatcacct cttgcacctc ttcgcttctt agctctatct
      601 ctttgtattt cttttcttct tccatttgtg ttgtaccgtc ttattgtcca agttcaagct
      661 gattcttcac cagctgataa tacttccctt ttgttgcagt aagctcctgg tgcgtgccct
      721 cctcaacaat cttaccccca tcaagaacca caatcttgtc ggcatcccgt accgtactga
      781 gtcgatgagc cactaccacc acagttttac ctttataaaa ctcattcaag tgctccatta
      841 tctccttttc attgttggcg tccaatgcat tggtagcctc atccagaaaa ataaactccg
      901 gatttttata tacagcgcgt gcaataagca tccgttgccg ctgtccctga ctgattccat
      961 taccttccat acctatcttg gtattatacc ccaacggaag cgaatcaata aaatcgcgga
     1021 tgtttgccac tgtaaccgca tggcgtaaac gttccggatc tatcatctcc tgcccggcag
     1081 caatatttcc ggcgatagag tcggaaaata taaacccatc ctgcataaca gagccggttt
     1141 tactacgcca catatgcgga tttaaatgct tcaagggggt ttctcccaat ttgatggatc
     1201 ccttgttggg ttcataaaac ccaagcagca acttaatcag cgttgtcttt ccactaccac
     1261 tcgcaccaac aatagcagtc accttgtttt caggtatggt aagattgata ttctccaaca
     1321 cataatcgcg g
//
LOCUS       DAOQXQ010000570         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_131005_length_1322_cov_3.418311, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000570 DAOQXQ010000000
VERSION     DAOQXQ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131005_length_1322_cov_3.418311"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKH81_08680"
     CDS             <1..225
                     /locus_tag="PKH81_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ98151.1"
                     /translation="HMGQSFRTYLNGLRIDYARMLLSEQPDTPIIDIIYDCGFNSKSV
                     FNAAFRASCGKTPSEWRKACLRTSDSESEE"
     gene            226..1107
                     /locus_tag="PKH81_08685"
     CDS             226..1107
                     /locus_tag="PKH81_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ98152.1"
                     /translation="MACPFCTSARSALVRTASSPTGSGEVCYYRCRSCASIYQYPLPS
                     PEGLCAYYERYLDVKASMNPGYLDSASVLSVRRERSLTLREIGFDPGRIIGGTNAELG
                     CANGLFLRYLVEEGSLRTIGLDLSESLLSAIVLSGVRLLRGGLELLDEKSVDNLYLFN
                     VAEHALDIEALFYQASRVLAQEGCVVLELPLSGLVSRFFGQRWRFLMPDEHLAIPSMK
                     GLRILLRKTGFTIRGQTRFGSGWTSGSIYRPIKRLCDFAAKHLGFGDRGCFLLEYRNP
                     VAPRFFGLIQGCIRTKK"
     gene            complement(1036..>1322)
                     /locus_tag="PKH81_08690"
     CDS             complement(1036..>1322)
                     /locus_tag="PKH81_08690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ98153.1"
                     /translation="HIRKTYIACRYGGDEFLVFLPGANELAARNIAESIRSDILNDFS
                     YSEEGEEIKITVSVGVSENRMDDSFDGLFLRADAALYQAKKTGRNRISVL"
BASE COUNT          290 a          306 c          348 g          378 t
ORIGIN      
        1 catatgggcc aaagttttcg tacctacctt aatgggctta gaatcgatta tgcccggatg
       61 ctcttgagcg aacaacctga taccccaata atcgatatca tttatgattg cggatttaat
      121 tccaaatcgg tgtttaatgc agcgttccgc gcttcttgcg ggaaaacccc aagcgaatgg
      181 cgaaaggcat gtttgcgtac gagtgattca gagtcggaag aatagatggc gtgtcctttt
      241 tgcacatcag cgcgttcagc tttagtacgt accgcttctt ccccaacagg gtcaggggag
      301 gtctgttatt atcgatgccg atcgtgcgct tcgatctatc aataccctct gccgtcacct
      361 gaaggccttt gtgcctatta cgagcggtat cttgatgtta aggctagtat gaacccggga
      421 tatcttgact ctgcttctgt tctttccgta cgtcgggagc ggtcgcttac tcttcgggaa
      481 attggttttg atccaggacg aataataggc ggaacaaacg cggagcttgg ctgtgccaat
      541 gggcttttct tgcggtattt ggttgaagaa gggagcctcc gaacgattgg cctggatctt
      601 tctgaatctc tgttgtcggc aatcgtcttg agcggagtac gactccttcg tggaggactc
      661 gagctacttg atgaaaagag tgttgataac ctctaccttt ttaacgtagc cgagcatgcc
      721 cttgatattg aagccttgtt ttatcaggct tctcgggttc ttgcccaaga aggttgcgtt
      781 gttcttgagt tgcctctttc gggcctggtc tcccgctttt ttggacagag gtggagattt
      841 ctgatgcccg atgaacattt ggctatccct tcaatgaagg gccttcgaat cctgttacgt
      901 aaaaccgggt ttaccatccg gggccagacc cgctttggca gcggatggac gagtggcagt
      961 atctacaggc cgataaagcg cttgtgcgac tttgcggcaa aacatctggg ctttggggac
     1021 agagggtgtt ttctgttaga gtaccgaaat ccggttgcgc cccgtttttt tggcttgata
     1081 caaggctgca tccgcacgaa gaaataagcc gtcaaaggaa tcgtccatcc ggttttcgga
     1141 cacacccacg ctgacggtta tttttatctc ttcgccttct tcagagtacg aaaaatcatt
     1201 gaggatgtcg ctacgaatag attctgcgat attgcgagcc gcaagttcat tagcaccggg
     1261 aaggaagacc aggaactcat cgccgccgta gcggcaggca atatatgttt tgcggatgtg
     1321 tt
//
LOCUS       DAOQXQ010000571         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_131124_length_1322_cov_1.921863, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000571 DAOQXQ010000000
VERSION     DAOQXQ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131124_length_1322_cov_1.921863"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKH81_08695"
     CDS             complement(<1..671)
                     /locus_tag="PKH81_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNQ98154.1"
                     /translation="MAGIAVVESASATKAPLAPWLIVDLGTNAEIALVTDTRIYASSA
                     SAGPAFEGGRISCGTGGIPGAVSSVRIEGSRFTYTTITREGMAVKPVGICGSGILDLT
                     ACALDTGLIEENGSLVPVCEQTGIVLDAQAGLRLTSADVREIQLAKAAIRAGVETLIH
                     EAGITEEAISTVYVAGAFGRYLQESSAIRTGLLPATFAGKIRAAGNTSLAGAVLALAG
                     PGTSG"
     gene            complement(708..1178)
                     /locus_tag="PKH81_08700"
                     /pseudo
     CDS             complement(708..1178)
                     /locus_tag="PKH81_08700"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          298 a          391 c          351 g          282 t
ORIGIN      
        1 tcgccagatg tgcccggccc ggcaagggca aggacagctc ccgcaaggga ggtgttccct
       61 gcagcgcgga tctttcccgc aaaggttgcc ggcaacaggc cggttcgaat cgcgcttgat
      121 tcctgcagat acctgccaaa ggctcctgct acatacacgg tcgagatcgc ttcctcggta
      181 atccctgctt catgtataag ggtttccact ccagcgcgga ttgccgcttt ggcaagctga
      241 atctctcgta catcggcgga tgttaaacgt aatccagcct gggcgtccag cactattccc
      301 gtctgttcgc acacgggcac caagcttcca ttttcctcta tcaagccggt atccagggca
      361 caggcggtca ggtcaagtat tcccgagccg cagataccaa ctggctttac ggccattcct
      421 tcacgggtta tggtggtgta cgtaaagcga gatccctcta tccgtaccga ggaaacagcc
      481 ccggggatgc ccccggttcc acaggagatt cgcccccctt caaaggctgg gcccgcggag
      541 gcagaggatg cgtatatgcg ggtatcggtt accagagcaa tttccgcatt cgtgcccaaa
      601 tccactatga gccagggggc taatggtgct ttcgtggcag aagcggactc gactactgca
      661 atgcccgcca ccaaatctcc tccggcaaag gcgcttattg ccggtataag acggacgggg
      721 caagaggccg caagcgggtt ttgcgcgcca aggacagcgg acgcatccat taccggaaag
      781 tctgtttgat ggggggtaaa gggagcgacg gcgagctcat ccaccggtaa gccaagcaag
      841 aggtgtacca tggcggtgtt cgcctagata cagagcgagc ctatcttccc cggactctcc
      901 tccgctcgct caagaatgag ccgtataagg gaacacaggt catcctgagc cgtctggcgc
      961 aagctttccc ccttgccaag ggttgccgca cgcaaacgag atattacgtc gcttccatag
     1021 cgtttctggc tattaagcat tgcttgttct gcccaatagt gtacgccgtc tccgtcgcta
     1081 agggcaccaa caatggtcgt tgtaccaagg tctactgcaa gacccaggtt cccctccacc
     1141 ttgcgcgcaa gagttgcttc tgcgcaagga gcgctcatta ccatcgctgc tgttcctctt
     1201 cgcctattga tcccttccac gcatacaagc caaggcgaac acatactgca agggcaatga
     1261 atgatattgg tatcaatgta gcggtaagac caagctcata aaaaccatga accagtataa
     1321 ca
//
LOCUS       DAOQXQ010000572         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_133630_length_1307_cov_3.585463, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000572 DAOQXQ010000000
VERSION     DAOQXQ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133630_length_1307_cov_3.585463"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>1307
                     /locus_tag="PKH81_08705"
     CDS             208..>1307
                     /locus_tag="PKH81_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ98155.1"
                     /translation="MKSYNKQSREEILAGIIETESHLNKIQDVDVLMEQILTEARRVV
                     HADAGSIYVRDGDRLAIHYAQNDTLQKQLPPGQKMLYSYFSFPINEKTIAGYSALTDQ
                     LVNEPDVYEIPLEKPYKFGRQTDVVSGYRTVSNLTIPLRTSGGRILGVLQVLNALDES
                     GNVTFFDKDDELYMSHFASNATVALEHAHLTRSMVLRMIKMSELRDPKETGPHVNRVS
                     SYAVEIYDRWAFNNAISENESKKYRDTLKIASMLHDVGKVAISDLILKKPARFTPEEF
                     EVMQGHTWLGAKLFNEIESPVDKIAREVALTHHENWDGTGYPGAVRVENGEPIKINHD
                     TGKALGLKGEEIPISGRIVALADVYDALSCKRV"
BASE COUNT          387 a          260 c          302 g          358 t
ORIGIN      
        1 aagttggtaa tcaagcctgt gccggctgta gacataccgt ggaaacgaac cggttgagca
       61 ttttgcacaa tcgatttaat caaaaattcc ttttctattt gtgaaacatt ctgaatcatg
      121 atatatagta taccacggaa ccagggtgga cgcatcaaaa aaactattgt agtattgtgt
      181 tccatatcgc ttggcaggag cattttaatg aagtcataca ataaacagtc aagagaagag
      241 attcttgccg gaattatcga aactgaatca catttaaata aaatacagga cgttgacgta
      301 ttgatggagc agattttaac tgaagctcga cgtgtcgtcc atgcagatgc cggttcaatc
      361 tatgtacggg acggtgaccg tttggcgatc cattatgcgc aaaacgatac cctgcaaaag
      421 caacttcctc ccggacaaaa gatgttgtac tcgtattttt cttttcctat taatgaaaaa
      481 accattgcgg gatatagcgc cttaacagat cagttggtga atgaacccga tgtctatgaa
      541 attccgcttg aaaaaccata taaatttggc agacaaactg atgtcgtctc tggatatcgt
      601 accgtatcca atttaactat accgctcaga acctctggcg gcaggattct gggcgttctt
      661 caagtgctta atgccctcga tgaatcgggg aatgttacct tttttgataa agatgatgag
      721 ctgtacatga gccattttgc ttctaatgct acggttgccc tggaacatgc tcatttaacc
      781 cgttccatgg ttcttagaat gattaaaatg tcagaactcc gcgatcctaa ggagactggc
      841 ccccatgtta atcgagtatc aagctacgcg gttgagattt atgatcgatg ggcctttaat
      901 aacgctatct cggaaaacga aagcaagaaa taccgggata ccttaaaaat cgcttcaatg
      961 ctccatgatg tgggaaaagt tgctatatct gatcttatac ttaaaaaacc tgcccggttt
     1021 accccggaag aatttgaggt tatgcaggga catacctggc tcggagccaa gctgtttaac
     1081 gagattgagt ctcctgtaga taagatagcg cgagaagtag cgcttactca tcatgagaat
     1141 tgggatggaa ctggctatcc tggagcggtg agggtagaga atggtgagcc gattaaaata
     1201 aaccatgaca ctggcaaggc tctagggctt aagggagaag aaatccccat aagtggtaga
     1261 attgttgcgc ttgcagatgt gtatgacgcg ctgtcctgta agcgtgt
//
LOCUS       DAOQXQ010000573         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_134195_length_1304_cov_2.663731, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000573 DAOQXQ010000000
VERSION     DAOQXQ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134195_length_1304_cov_2.663731"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1304
                     /locus_tag="PKH81_08710"
     CDS             <1..>1304
                     /locus_tag="PKH81_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNQ98156.1"
                     /translation="GFLAKRMELGAVIAAAPLSHVVREEPENGDIVILVGGGTGRDGI
                     GGATGSSKVHTEESVTTAGAEVQKGNAVEERKIQRLFRDGSVTRLIKRCNDFGAGGVA
                     VAVGELASGLDIDLDSVPKKYEGLNGTELAISESQERMAVVVREEDALAFIKAANGEN
                     LNAAIIARVTGNNRLVMRWQGQTIVDIDRAFLDTAGAQRNAKVSIEKPGPAWDWGTAK
                     GTGKDRLLSVLSHLSVAGQRGLTERFDGSIGSSAILFPFGGTYQATPAAGMASRIPVG
                     PGKETTTASLMTMGYDPKALSWSPFHGAQYAVLESLTKILALGGDPRKARLTFQEYFE
                     RTSSSQAWGKPTAALLGALTAQLEAGVPAIGGKDSMSGTFGELSVPPTLVSFAVATTD
                     QALVRDGALTTSGKQLVLIMTPWKEDKTPDWQAFRSIAKDLL"
BASE COUNT          311 a          367 c          365 g          261 t
ORIGIN      
        1 ggatttttgg caaagcgaat ggaacttggc gccgttatcg ccgccgcccc gctttcccat
       61 gtagtgcggg aagaacccga aaacggagac attgtcatac tcgttggcgg cggaaccggt
      121 cgcgacggaa tcggcggagc aaccggttct tccaaggttc acaccgagga gtcggtaacc
      181 acagccggtg ccgaggtaca aaaaggtaac gcggtggaag agcgaaagat tcagcggctg
      241 ttccgcgacg gctctgttac ccgccttatc aagcgctgta acgattttgg ggccggtggt
      301 gtcgccgttg ccgtaggaga gctcgccagc gggctggata tagatcttga ttcagtacca
      361 aaaaaatacg aaggacttaa cggaacggaa ctcgccatat cagagtccca agagcgaatg
      421 gccgttgtag tgcgcgaaga agatgccctt gcctttatca aggctgccaa cggcgaaaac
      481 ctgaacgccg cgattattgc gcgggtaacg ggaaacaacc gactggttat gcggtggcaa
      541 ggacaaacaa tcgtagatat tgaccgcgcc ttcctcgata cagccggagc gcaaaggaat
      601 gccaaggtct ccatagaaaa gcctggccca gcatgggact ggggcaccgc caagggcact
      661 ggcaaggatc ggctgctttc ggttctgtcc catctttcgg tagccggtca acgagggctt
      721 acagaacgct ttgacggatc tatcggttcc tcggccatcc tctttccctt tggcggtacc
      781 tatcaagcca caccagcggc cggtatggcc tcgcgcattc ccgtaggacc gggaaaggaa
      841 acgacaaccg caagcttgat gactatgggc tatgatccaa aagccctttc gtggagtccc
      901 ttccacggcg ctcaatacgc tgttcttgaa tctcttacca aaatactggc cctgggtgga
      961 gacccgcgca aggcacgcct aacctttcag gaatattttg agcgaacttc ctcgagtcag
     1021 gcatggggaa aacccaccgc agcacttttg ggagccctga ccgcccagct cgaggcagga
     1081 gtgccggcta ttggcggaaa ggacagcatg tccggcacct ttggggaact ttccgtccct
     1141 ccaaccctgg tttcgtttgc cgtcgcaaca acggatcaag ctcttgttag agacggagcc
     1201 cttaccacga gcggcaaaca gctcgtcctg ataatgactc cctggaagga agataagact
     1261 ccagactggc aagccttccg ctccatagcc aaagaccttc ttca
//
LOCUS       DAOQXQ010000574         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_134222_length_1304_cov_2.431545, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000574 DAOQXQ010000000
VERSION     DAOQXQ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134222_length_1304_cov_2.431545"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKH81_08715"
     CDS             <1..1057
                     /locus_tag="PKH81_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="neuraminidase"
                     /protein_id="HNQ98157.1"
                     /translation="NDKIIQSEPHSVIYRAAGPWITIDNLIMGDFAIERPWLMGTAGY
                     SLNEEEALALRSKETSKEEKRRIQAKTVEKVEISFDNGKTFIPTEAGKKWRYRLETGD
                     LREGYHFMVVRATMKTGEVTVSRSIIQVDKTPPTIRLISPGEGGRYNNELVFSGLSSD
                     DVQLDSVMLSLRPGDKSAYAIPAFIQGLYFDWHFWGATLYDIGVGLTFFDNNVKLQVQ
                     FGQFTDEQRAVFTDSTMRYGGNVFGLKLLANLLYLPMDYFFGPDFYWLSASAAIGANF
                     SIFTETQSGQPQILSAILFQLEFPRVTMPKRKTFKTFSLYTEGQFWFIPTDIDSSEVS
                     VDSVLPHITAGIRLNVF"
     gene            1100..>1304
                     /locus_tag="PKH81_08720"
     CDS             1100..>1304
                     /locus_tag="PKH81_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98158.1"
                     /translation="MKFTKTSVFGLVSVFLLSALSLTCTIGLGDSVDTKAPQVAIAYP
                     PVQSIIRNTFTMNGTASDDVKLLG"
BASE COUNT          312 a          307 c          313 g          372 t
ORIGIN      
        1 aaacgacaag attattcagt ccgaacccca ttcggtgata taccgtgcag ccggtccctg
       61 gattaccatc gacaatttaa tcatgggaga ctttgccata gagcggccct ggctaatggg
      121 tactgccggc tattcactta atgaggaaga agcccttgct cttagatcca aggaaacttc
      181 caaagaagaa aagcgacgta ttcaagccaa gactgttgaa aaggtagaga tcagctttga
      241 taatggaaag acctttattc ctacggaggc cggaaagaaa tggcgctatc gtctggaaac
      301 cggcgatctt cgcgagggat atcactttat ggttgttcgc gcaacaatga aaaccggaga
      361 ggttactgtt tctcgatcga ttatccaggt agataaaacg ccgccaacta ttcgccttat
      421 ttctcctggc gagggtggac gatacaacaa cgaactggtg ttctccggtt tgagtagcga
      481 cgatgttcaa ctggactccg ttatgctctc gcttcgccct ggggacaagt ctgcctatgc
      541 aataccggca tttattcaag gtctgtactt tgactggcac ttctggggag ccacgctcta
      601 tgatattggc gtgggactta ccttctttga caacaacgta aaactccagg tgcagtttgg
      661 acagttcacg gatgagcaga gagccgtctt taccgactct accatgcgat atggcggaaa
      721 cgtctttggc cttaagctgc ttgccaactt gttgtatctg ccgatggatt acttctttgg
      781 gcctgacttc tattggcttt ctgcctctgc agctattggc gctaacttct ccatctttac
      841 agagactcag agcggtcagc cgcaaatcct gtctgcgata ctcttccagc ttgagttccc
      901 ccgggtaacc atgcccaagc gtaagacgtt caagaccttc tctttgtata cagagggaca
      961 gttctggttt atcccgacgg atatagattc atccgaggtt agtgtagatt cggtgttacc
     1021 gcatattact gccggtatca gacttaatgt gttttgatag ttattttttg taagaaatat
     1081 gtcagtaagg aaggtatgta tgaagttcac taaaaccagt gtgttcgggt tggtgtcggt
     1141 atttttgctt tcggcattgt cccttacctg taccattggc cttggcgatt ctgttgatac
     1201 caaggctcct caggttgcta ttgcctatcc accggttcag agcatcattc gcaatacctt
     1261 taccatgaac ggaaccgctt ccgacgatgt taagctcctg ggtg
//
LOCUS       DAOQXQ010000575         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_134888_length_1300_cov_3.612048, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000575 DAOQXQ010000000
VERSION     DAOQXQ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134888_length_1300_cov_3.612048"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..276
                     /locus_tag="PKH81_08725"
     CDS             100..276
                     /locus_tag="PKH81_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98159.1"
                     /translation="MSKAHRGKGIRGNPNRGRGTCPVCKKEGVKVLYEQEIDGAKTVI
                     CKVCKATLKNGKAS"
     gene            440..>811
                     /locus_tag="PKH81_08730"
     CDS             440..>811
                     /locus_tag="PKH81_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98160.1"
                     /translation="MKVSKIVCVALLACVVMGVHAAGQKEANVIKIGVAGAHSGDLAS
                     YGLPSVNAAKLVVEEYNAKGGINGKQVELVIVDDQCKPELATNAASKLVSDQVVAVVG
                     HICSGATKSALGIYKDSGIVCI"
     assembly_gap    812..821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <822..>1300
                     /locus_tag="PKH81_08735"
     CDS             <822..>1300
                     /locus_tag="PKH81_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ98161.1"
                     /translation="LTQSGEYPNFFRSIAPDDAQARLAVTFIKNNLKLTSLAVLHDKG
                     DYGKGFADLVKEYAEKEGIKVLLFEGVNPGAPDYSAVVNKIGNAAPDGVVWGGYHPEA
                     SKLVQQMRDKGMKIPFVSDDGVKDNTFIEVAGKYAEGVYATGPMDTSKLPMAVKAIN"
BASE COUNT          368 a          262 c          345 g          315 t
ORIGIN      
        1 aaggctggag cgactaaaaa aagtgctgga gaaaagaaag ctccggctgc taagaaaagc
       61 gcacccaaaa aagcggccga agccggggag gagtaaggta tgtccaaggc acatcgcgga
      121 aagggaattc gcggcaatcc taacaggggt cgcggtactt gcccagtttg caaaaaagag
      181 ggcgtaaagg ttctttacga gcaggaaatc gacggagcaa aaaccgttat ctgcaaggtt
      241 tgcaaggcaa ccctcaagaa tggcaaagca agctaatagt ttttttgtca taataaaacc
      301 gtccactccg gacggttttt ttttgggcaa agcaggtttc agtgctgtaa ataggtggac
      361 acggcgtaga agtacgccta aaataacatt atatatccac tattaaagta atggatataa
      421 aagaggagag gaggaataca tgaaagtatc caaaattgtg tgtgtcgccc ttttggcttg
      481 tgttgtcatg ggtgtccatg cagcaggaca gaaagaggca aatgtaatca agattggtgt
      541 tgctggagcc cattcaggcg atcttgcgtc gtatggattg ccctcggtta acgcagccaa
      601 acttgttgtg gaagaatata atgccaaggg cggcattaac ggtaagcagg tagaacttgt
      661 tattgttgac gatcagtgta agcccgaatt ggcaaccaac gccgcttcca aactggtgag
      721 cgatcaggtt gtagctgttg tcggtcatat ctgttccggt gccaccaaga gcgcgcttgg
      781 tatttataag gactcgggaa ttgtatgtat tnnnnnnnnn ntgttaaccc agagtggaga
      841 atatcctaac ttcttccgct ctattgctcc ggacgacgcc caagctcgtt tggcagtaac
      901 ctttatcaag aacaatctta agcttacatc tcttgctgta ttgcacgaca agggtgatta
      961 cggcaagggc tttgccgatc tggttaaaga atatgccgag aaggaaggca ttaaggttct
     1021 tttgtttgaa ggtgttaacc ccggtgctcc cgattattca gccgttgtta acaaaattgg
     1081 gaacgcggct cccgatggcg ttgtatgggg cggttaccac cctgaagctt caaagctcgt
     1141 acagcaaatg cgcgacaagg gtatgaagat cccctttgtg tctgacgacg gtgtaaagga
     1201 taataccttt atcgaagttg ccggaaaata cgccgaaggt gtatatgcaa ccggaccaat
     1261 ggataccagc aagctgccga tggctgttaa ggctattaac
//
LOCUS       DAOQXQ010000576         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_136192_length_1293_cov_2.546042, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000576 DAOQXQ010000000
VERSION     DAOQXQ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136192_length_1293_cov_2.546042"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKH81_08740"
     CDS             complement(<1..452)
                     /locus_tag="PKH81_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ98162.1"
                     /translation="MKDTLMKKTYIIGHRNPDTDSIVSAAAYTALKKAQGYSGCVAAR
                     AGKPTPQTEYIFNRFKVPLPEFVSDLVPKVAYHYNPDITTIGEETSLWEAMEILGSGT
                     VRVLPVVDGEGKYRSLLHYSFLAQKVFKMINPKQKTVIQTSIELLSSV"
     gene            504..1097
                     /gene="pth"
                     /locus_tag="PKH81_08745"
     CDS             504..1097
                     /gene="pth"
                     /locus_tag="PKH81_08745"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNQ98163.1"
                     /translation="MISLVAFLGNHGKTYRHNRHNVAWLFADSLSLAASLSWQSKFKG
                     RYASCSIAPLDGGPRRVVHFLKPETFMNLSGESVAEAAAFYRIPPADILVVHDELELP
                     PGTVSLKWSGGLGGHNGLRSLEASLGTADFWRLRFGIGRPVHGDVASFVLSDFTGDEQ
                     ILMSQVWSLSEDLFTRTLSSEPQHLLPEWAKKRLVEP"
     gene            1110..>1293
                     /locus_tag="PKH81_08750"
     CDS             1110..>1293
                     /locus_tag="PKH81_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNQ98164.1"
                     /translation="MNTPLSAETMSAFNRLYETIIRLRAPGGCPWDIEQTPKTLRETL
                     IEEAYETVEAITDEDPE"
BASE COUNT          300 a          331 c          342 g          320 t
ORIGIN      
        1 aataccgagg aaagcaattc tatgcttgtc tgaataacgg ttttttgctt ggggttaatc
       61 atcttgaaga ctttttgggc aagaaagctg taatggagca gcgaacggta cttgccctcg
      121 ccatccacaa cgggaagtac ccgaaccgta cccgatccca gtatttccat ggcttcccac
      181 agggatgttt cttcgccgat ggtggtgatg tccggattat agtggtaggc aaccttgggt
      241 accagatccg agacaaactc tggaagggga accttaaaac ggttaaagat atattcagtt
      301 tgcggcgtag gtttacccgc gcgggcggcg acacagcctg agtatccttg cgccttttta
      361 agcgcggtgt aggcggcggc tgagacaata gagtcggtat cgggattacg gtgcccgatg
      421 atataggttt tcttcatgag tgtgtctttc acgttgctgg aagtatacac taaaacagaa
      481 gttttcggta caatggccgc atcatgatct cgcttgttgc cttcctggga aaccacggaa
      541 aaacctaccg tcacaaccgt cacaatgtcg cctggctttt tgccgatagc ctgtctcttg
      601 ccgcttctct ttcctggcag tccaagttca agggccgtta tgcctcttgc tctatcgctc
      661 ccctggacgg agggccacgc cgtgtggttc attttcttaa accggaaacc tttatgaatc
      721 tttccgggga aagcgtggcc gaagccgccg ccttttaccg cataccaccc gccgacatcc
      781 tggtggtgca cgatgaactt gaacttcccc cgggaacagt gagccttaag tggtcaggcg
      841 gacttggcgg gcataatgga ctgaggtctc ttgaggcctc gctcggtacg gcagactttt
      901 ggcggctacg atttggaatt ggccgcccgg tccatggaga cgttgcctcc tttgtacttt
      961 cggattttac cggcgacgaa cagatactga tgtctcaggt gtggtcctta agcgaggatc
     1021 tttttacccg cacactcagc tcggaaccgc agcatcttct gcctgaatgg gcaaaaaaac
     1081 ggcttgtgga gccgtaaagg aataccgtca tgaatactcc gctttccgca gaaacgatga
     1141 gcgcctttaa ccgattgtac gaaacgatca tacgtttacg cgccccaggg ggctgtccct
     1201 gggatataga gcaaacaccc aagaccctta gggaaacgct tatagaagaa gcatacgaga
     1261 ccgtagaggc tataaccgac gaagatccgg aac
//
LOCUS       DAOQXQ010000577         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_136219_length_1293_cov_2.281099, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000577 DAOQXQ010000000
VERSION     DAOQXQ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136219_length_1293_cov_2.281099"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKH81_08755"
     CDS             complement(<1..697)
                     /locus_tag="PKH81_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98165.1"
                     /translation="MAKTIDDKKIIYTMDRVSKNHGTKQVLKDISLSYFYGAKIGVIG
                     LNGSGKSSLLRILAGTDTEFLGETSCAPGYTIGFLEQEPHLESGKTVKQIVAEGAKET
                     MDLLAEFDSINEAFGDPDADMDKLMERQSRVQDQLDALNAWELESQLELAMDALRCPP
                     ADQVVDVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAETVAWLERHLQHYEGTII
                     AVTHDRYFLDNVAE"
     gene            complement(796..>1293)
                     /locus_tag="PKH81_08760"
     CDS             complement(796..>1293)
                     /locus_tag="PKH81_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ98166.1"
                     /translation="AADETNLYYLMKIADSKVITGKHGKDFWNEDSMEFYFNFTGNLA
                     TKAYAPGIAQINIPAINIGKKAGDAITVIGSNSETVKVNAAVVKTADGWMFEASVSLA
                     NVNPEHGLTIGIQAQANGATEKDRDTKLIWSKKDKGDGSYQDPSLFGKGVFFKVGSAD
                     TPKAQ"
BASE COUNT          314 a          300 c          302 g          377 t
ORIGIN      
        1 attcggcaac gttatccaaa aaataacgat cgtgggtaac ggcgataata gttccctcat
       61 agtgttgaag gtgtctttct agccatgcga cggtttctgc atccaaatgg tttgttggtt
      121 cgtcaagaag caagatgtcc ggtttttgta aaagaaggcg gcataaggca actcggcggc
      181 gctctccacc agagagaacg tcaacaactt ggtcagcggg aggacagcga agcgcatcca
      241 tcgcgagttc aagctggctt tccagctccc atgcgttgag cgcgtcaagt tgatcctgca
      301 cgcgcgattg gcgttccata agcttgtcca tatctgcgtc gggatcgcca aaggcttcgt
      361 ttatcgagtc aaattctgca aggagatcca ttgtctcctt ggctccttcg gcgactattt
      421 gttttacggt ctttccactt tcaagatgag gctcttgttc caaaaaacct atggtgtatc
      481 cgggcgcaca ggaagtttct ccaagaaact cagtgtctgt tcctgcaaga attctaagga
      541 gacttgattt tcccgaacca tttaatccga taacaccaat ctttgctcca taaaaatagg
      601 atagactgat gtctttaagt acttgctttg tcccgtgatt ttttgaaacc cggtccattg
      661 tataaatgat tttcttgtcg tcaattgtct ttgccataga cttgagtgta ctcaaaacag
      721 taaaaagatg ctatggcggt aaacgataaa aaaagccgcc ccaacgggcg gcctttattg
      781 gtcggggatg taagcttact gagctttagg agtgtctgcg cttcctactt taaagaagac
      841 tcctttccca aataaagagg gatcctggta tgagccgtcc cccttatctt tcttgctcca
      901 gatcagtttt gtatctcggt ctttttcggt tgcgccattt gcctgtgcct ggataccgat
      961 ggtaaggccg tgctccgggt tgacgttagc aagcgaaaca gatgcctcaa acatccaccc
     1021 gtcggctgtc ttgacaacag cggcattaac cttaacggtt tcggagttgc taccaattac
     1081 tgttatagcg tctccagcct tcttgccgat gttgatagcg ggaatattaa tttgggcgat
     1141 gccaggggca tatgcctttg tagccaaatt cccggtgaag ttaaaataga actccatgga
     1201 atcttcgttc caaaaatctt ttccatgctt accggtaatg acttttgaat cggcgatctt
     1261 cataaggtag tagaggtttg tttcatcggc agc
//
LOCUS       DAOQXQ010000578         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_136389_length_1292_cov_2.329022, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000578 DAOQXQ010000000
VERSION     DAOQXQ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136389_length_1292_cov_2.329022"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="flgK"
                     /locus_tag="PKH81_08765"
     CDS             complement(<1..517)
                     /gene="flgK"
                     /locus_tag="PKH81_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688407.1"
                     /note="with FlgL acts as a hook filament junction protein
                     to join the flagellar filament to the hook; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNQ98167.1"
                     /translation="MSTFSGIEMGKRSLFAHSQSMQTAGHNLSNSSTEGYSRQRVELK
                     ATDPLYRPDLSRVETPGQLGQGVSVQSIRRMRDELLDQRIVAQTNSEGYWQTRDNYLL
                     MMEQVYNEAGDTSVRTRMDQFWDSWEELSLYPESRSARQAVLARGETLTDAIHQQYRG
                     LKGIRDIIHGDV"
     gene            complement(531..1028)
                     /locus_tag="PKH81_08770"
     CDS             complement(531..1028)
                     /locus_tag="PKH81_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98168.1"
                     /translation="MNDAWVYMTAIERVLEQETALLLSLYAGQKRLSESVVARDWRAL
                     ESEIHLCSSLADKFNEIEQERLDLVANALGQKEAVNGFYRLTSFFPDADRTRINALYR
                     EVKRLLLLCRTESEVFTSYVSNAKSLLSGMFDTVLSGGAGKVYTKSGNLASRTLDSVV
                     LNRSF"
     gene            1159..1230
                     /locus_tag="PKH81_08775"
     tRNA            1159..1230
                     /locus_tag="PKH81_08775"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1191..1193,aa:His,seq:gtg)
BASE COUNT          334 a          328 c          305 g          325 t
ORIGIN      
        1 caacgtcacc atgaatgatg tccctgatgc ccttgaggcc ccggtactgc tggtgaatgg
       61 cgtcggtgag ggtttctccg cgcgccagaa ccgcctgacg ggcgctccgg gattcgggat
      121 acaaggaaag ctcttcccag gaatcccaaa attgatccat gcgggttctg accgatgtat
      181 cgccggcttc gttatatact tgctccatca ttaacaggta gttatctctg gtttgccaat
      241 aaccttcgct attggtttgc gcaacaatgc gttgatctaa aagttcatcc ctcattcgtc
      301 taatggactg gacagataca ccctgaccaa gctgtccagg agtttcaaca cgtgaaagat
      361 cgggacgata gagcggatca gttgccttaa gctctaccct ctggcgtgaa taaccttcgg
      421 tagatgaatt ggaaaggtta tgaccggcag tttgcattga ttgcgagtga gcgaataagc
      481 ttcgcttacc catttctatt ccggaaaagg tagacatcta ctactcctta tcaaaacgaa
      541 cggtttagga caacactgtc cagagtgcga gaagcgagat ttcccgactt ggtgtacacc
      601 ttaccggcac cacctgaaag taccgtatcg aacatcccgc tcaaaaggct ctttgcgtta
      661 ctgacatacg aggtaaagac ttcgctttct gttctacata aaagaaggag gcgtttaacc
      721 tcccgataca gggcattgat gcgagttcgg tctgcatcgg ggaaaaatga ggttagtcgg
      781 taaaagccgt taaccgcctc tttctgtcca agtgcgttag ctacaagatc aagacgctct
      841 tgttcaattt cattaaactt gtcggcaagg cttgaacaca gatggatttc actttccaga
      901 gcccgccagt cgcgcgcgac aacagattcg ctaagccgtt tttggccggc atacagggac
      961 agtaaaaggg ctgtttcctg ttccaaaacc cgttcaattg ccgtcatata tacccatgcg
     1021 tcattcattg tgcacactcc ttcaactcat tatcggaact aacagatcaa ttcctgagac
     1081 aaagaaagca aaaacgataa aatcatcgat attgacattt tcacccgctt ctgccattat
     1141 gggcctcgtc aattctcggt gggtgtagtt caggggtaga gcaccagatt gtggatctgg
     1201 ctgtcgcggg ttcgaatccc accacccacc ctctgaaaac gcccgctggg agaagtccca
     1261 aaagggcttt tcggccgaaa aactggacga ga
//
LOCUS       DAOQXQ010000579         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_136919_length_1289_cov_2.573744, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000579 DAOQXQ010000000
VERSION     DAOQXQ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136919_length_1289_cov_2.573744"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1289
                     /locus_tag="PKH81_08780"
     CDS             <1..>1289
                     /locus_tag="PKH81_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNQ98169.1"
                     /translation="NTGGKTVTLKTAALLSLINQTGWPIPAAEGSALPIFDAVYCDIG
                     DEQSLSQSLSTFSAHMKNIASILEHATDNSLILLDELGSGTDPQEGSAIAMAVLDRLL
                     AVSSLVLITTHHGILKNYGYTHAQCVNASVDFDENTLSPTYRILMGVPGESHALDIAR
                     RNGLDGEVVDHARQHLIEERADVSALIKGLTAKHEELEGFEQVKKKEEAALREKRRKT
                     DLKELQLRQKELELREAGYRRLETLFDESRKQLENLVREIREGELTREKTQQVKAWMA
                     SFEELVEGEKDGLRLCREETQSARRALEQTQAQEAASRSQDAPFSRSRRGTKKVPGVS
                     SPVSAKLPDLAPGIEVYIGENRRLGVLVREEKGGKWIVATGQLRMTVRSEEIQAVPLR
                     PGMEKNRIEVHTEMGSDDSPVFELKLLGMRHDEALKA"
BASE COUNT          332 a          305 c          351 g          301 t
ORIGIN      
        1 caaataccgg cggtaaaacg gttaccctaa aaacggcggc acttttatcc ctgattaacc
       61 aaaccggctg gccaattcct gctgccgaag gaagtgctct tccgattttt gatgcggtgt
      121 attgcgatat tggcgacgag cagtctctgt ctcaatctct ttctaccttt tccgctcaca
      181 tgaagaatat cgccagtatt ttagaacacg caaccgacaa cagtcttatt cttcttgatg
      241 agcttggtag tggtaccgat ccccaagagg ggagcgctat cgccatggcg gtgttggacc
      301 ggcttcttgc tgtgtcttct cttgtgctta ttacgaccca tcatggcata ctcaaaaatt
      361 atggatatac ccatgcccag tgtgttaatg cctcggttga ctttgatgaa aacaccttga
      421 gtcctaccta ccgtattttg atgggcgttc ctggggaaag tcacgccctg gacattgccc
      481 gccgaaacgg tctggacggc gaggtagtcg atcatgctcg acagcattta atcgaagagc
      541 gggcggatgt gtcggccctg attaagggct tgacggcaaa gcacgaggaa cttgagggtt
      601 ttgaacaagt caagaaaaag gaagaagcag ccctccgcga aaaacggcga aaaactgatt
      661 tgaaagaact acagctacgt caaaaagaat tggaactgcg cgaagcaggc tatcgccgac
      721 ttgaaacatt gtttgacgaa agtcgtaaac agctggaaaa tctggttcga gaaattcgtg
      781 agggtgagtt aacaagagag aaaacccaac aggtaaaggc atggatggca tcctttgaag
      841 agcttgttga gggtgaaaag gatggcttac ggctgtgccg cgaagaaacc cagtcggcca
      901 gaagagcgct tgagcaaact caggcgcaag aagcggcttc tcgctctcag gatgcccctt
      961 tttcccgttc gcgtcggggc acaaaaaagg tccccggggt ctcttcccct gtgtctgcaa
     1021 agcttcccga tttagcgccc gggattgagg tgtacattgg cgagaatcgc cgccttggtg
     1081 tgttggttcg cgaggaaaaa ggcggtaaat ggattgttgc taccggacag ctgaggatga
     1141 ccgttcgctc tgaagaaatt caggccgttc cgcttaggcc gggaatggaa aaaaaccgga
     1201 tagaggtcca caccgagatg ggtagcgacg actctcccgt ctttgaactt aaactcttgg
     1261 gtatgcgcca tgacgaggcc ttaaaggcc
//
LOCUS       DAOQXQ010000580         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_137107_length_1288_cov_2.720195, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000580 DAOQXQ010000000
VERSION     DAOQXQ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137107_length_1288_cov_2.720195"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKH81_08785"
     CDS             complement(<1..348)
                     /locus_tag="PKH81_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98170.1"
                     /translation="MKKRTIFYLLVVIGTLLHLEAQETRTVKADLLGNGSQQELSFSL
                     GNNGNSGFYGFSLTNNGYTKAVFNRKNISDYCDPDDFFVGGVSAYSQADFIVGGLVAV
                     IRQKSRALLWLTRD"
     gene            complement(663..1163)
                     /locus_tag="PKH81_08790"
     CDS             complement(663..1163)
                     /locus_tag="PKH81_08790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ98171.1"
                     /translation="MKTNAQNTKSDNQEKRAESYSVGKLIYDPKVYDGLNRHTEDISF
                     YRTWIKDQHICKVLEVCCGTGRIAIPLAQSGVEMTGLDLNESMLSEARSKAEKANVPI
                     DFHQGDMRRFSLGKQYQLVFIPFNSIHCLYTHEDITNTFASIRAHVADGGYFIIVILT
                     PISRIS"
BASE COUNT          364 a          296 c          243 g          385 t
ORIGIN      
        1 gtcgcgggta agccatagca gggcccggct cttttggcgg ataaccgcca ccaatccccc
       61 aacgataaag tcagcctgcg aatatgcgct tacgccccca acaaagaaat catcgggatc
      121 gcaatagtcg ctaatgttct tccgattaaa aaccgctttt gtatacccgt tgtttgttag
      181 cgaaaaacca taaaatcctg agttaccatt gttccccagg gaaaatgaaa gttcctgttg
      241 gctcccgttt cctagaaggt ctgctttaac ggtacgtgtc tcctgtgcct ccagatgcaa
      301 cagtgtccct atcactacta gtaagtaaaa aatcgttctc tttttcattc ctcaatcctt
      361 atgtattttt ctacaaacta taagctgcgt aggggatcca gaaataaatg gattcatttg
      421 ataatctccg tatttcgcag caatggtaaa accacagctt ttaatataat agtcaagctc
      481 ttgaggataa aacattctca tatcaaggga ttcgaccgaa tgagacctac cattaacgat
      541 gtgttcccat ttgattctgt ttatttgcga atctctttca tagtgcatgc tttgtttaat
      601 gcaaatgtct ctaccgtcat cggtgacgaa attggcaatt tccatcgatg acccctgatt
      661 ctctacgata tacgagatat cggggttaaa ataacaataa taaaatagcc cccatccgct
      721 acatgcgcac gaatagaagc aaaggtattg gtaatatcct catgagtata taaacaatgt
      781 attgagttaa acgggataaa cacaagttga tattgtttgc ctaaagaaaa acgcctcata
      841 tcgccttgat gaaaatcgat cggtacattt gccttttccg ccttacttct ggcctcgctg
      901 agcatcgact cattaagatc caagcctgtc atttctacac ccgattgggc aagaggtatg
      961 gctatccgcc ctgtcccgca gcatacttcc aggaccttgc agatatgttg atctttaatc
     1021 caggttcggt aaaaggagat atcttccgta tgacggttta acccgtcata caccttggga
     1081 tcatagataa gcttcccaac tgagtatgat tcggctcgct tttcttgatt atcgctcttg
     1141 gtgttttgtg cattagtttt catatatctc catcattata tcattaaata attagcatag
     1201 aaaagcgttt gacttaccat gaatctttac ggatacttct acggcgggga caacgtgatc
     1261 cgactagata aggactacca atatgcac
//
LOCUS       DAOQXQ010000581         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_138146_length_1282_cov_3.327628, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000581 DAOQXQ010000000
VERSION     DAOQXQ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138146_length_1282_cov_3.327628"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1282)
                     /gene="hydF"
                     /locus_tag="PKH81_08795"
     CDS             complement(1..>1282)
                     /gene="hydF"
                     /locus_tag="PKH81_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNQ98172.1"
                     /translation="LMIESPKSVRLHIGIFGRTNSGKSSILNLITAQETSIVSPIAGT
                     TTDVVRKSMELLPLGPVTFLDTAGIDDSGQVGELRVKRSREALSSSDIVLLVCEVNCW
                     TAYEEEVLSLCKAQNTPVIILVNKVDTSSPSESFTALIKQNDSRTVFLSALNAQRDAQ
                     ARENFMSSLKHLLITLCPESFLEPPAILGDLLPSGSSLPLVVLVVPIDLQAPKGRLIL
                     PQVQSIRDALDSDAAVLVVKEREMRALLDSLSRKPDLVVCDSQVALKMVADTPDDVPC
                     TTFSILFSRFKGNIETMAAGAAAINKLQHNDKVLMAEACTHHALEDDIGRVKIPRWLR
                     EYTGLELDITHACGKDYPQDLSSYSLAIHCGACTLTRREMLWRIEQARLSGVPVTNYG
                     MAISALQGVIERTLSPFPSALEAYRTALAKGGTD"
BASE COUNT          334 a          289 c          344 g          315 t
ORIGIN      
        1 tcaatcggtt cctcctttgg caagggcggt tctatacgct tccagagcag aggggaaggg
       61 ggaaagcgtt cgttcaataa ctccttgcaa ggcagatatt gccatgccgt aattggtaac
      121 gggaacgccg gagagccttg cttgttcaat tctccacagc atctcgcggc gggtcagggt
      181 acacgctccg caatgaatcg ccagagagta tgaagaaaga tcttggggat aatcttttcc
      241 gcaggcatga gtaatgtcta attcaagtcc tgtgtattca cgcagccagc ggggaatttt
      301 taccctgcca atgtcatctt ccagtgcatg gtgcgtacag gcctccgcca tcagtacctt
      361 gtcgttgtgt tgtagtttgt tgattgccgc ggcgccggcg gccatggtct caatattccc
      421 cttaaagcgg gaaaaaagaa ttgagaatgt ggtgcaagga acatcgtctg gagtgtcggc
      481 aaccatcttt agcgctacct gggaatcgca aaccaccagg tccggctttc tggaaagact
      541 atccagaagc gcgcgcattt cgcgctcctt tactacaaga acggcagcat cgctatcgag
      601 cgcgtcgcgg attgattgta cttgaggaag aatgagccgc cctttgggag cctgcaaatc
      661 gatgggaaca acaagaacca caagcggcag agaagatccc gaaggaagca ggtccccaag
      721 aatagctgga ggttcaagaa aggattcggg gcacagagta attagaagat gcttaaggct
      781 ggacataaaa ttttctcgtg cctgtgcgtc tctctgtgcg ttaagagcgg agaggaaaac
      841 tgtgcgagaa tcgttttgct ttataagggc cgtaaaggat tcggaaggag aagaagtatc
      901 aactttattg accagaataa taaccggcgt gttttgcgcc ttgcacagag acagcacttc
      961 ttcttcgtac gccgtccagc aattcacttc gcatactaaa agtacaatat ctgaactgga
     1021 gagggcttcg cgagatcgtt tgacacgaag ctctccaact tgacctgaat catcaatgcc
     1081 cgcggtgtca agaaaggtaa ctggtcccag tggtaaaagt tccatgcttt ttctaacaac
     1141 atcggtagtc gtccctgcaa ttggggagac aatggatgtt tcttgagcgg ttataagatt
     1201 gagaatgctt gatttgccgg agtttgttct accaaagatg cctatgtgga gacgtaccga
     1261 tttaggcgat tcaatcatga gg
//
LOCUS       DAOQXQ010000582         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_138218_length_1282_cov_2.327628, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000582 DAOQXQ010000000
VERSION     DAOQXQ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138218_length_1282_cov_2.327628"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKH81_08800"
     CDS             complement(<1..333)
                     /locus_tag="PKH81_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNQ98173.1"
                     /translation="MNAHKIAAQVWAVHADVHTDTRFEGIWPIPHGVTLNSYLVTGTK
                     TALIDLAKDWAGSIDQLETQLASAGSSFEKIDYLILNHLEPDHTAFIIEMRKRNPKVE
                     IISTPKGIS"
     gene            429..>1282
                     /locus_tag="PKH81_08805"
     CDS             429..>1282
                     /locus_tag="PKH81_08805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNQ98174.1"
                     /translation="MITALAPMATLTHEALRLLIHRFGDPTEYYSEMIHAPSYVCGGV
                     FEKWYVRTAPAPDKMVWQLTGPTASSMAQAAALLLPLGGLGIDVNMGCSAPDIVKTGC
                     GIAWMSKEGAEVRTLISSIRSCIDQYLTDNPGQQRRLGVKLRLGEDEDYTRLLDFCRM
                     LVDEGVEQLTLHPRTRREKYGRPPRHGYTVRLGQDIGVPVYGNGAISSAGEAVSFMEK
                     YPCSGLMIGRAAITRPWIFRQIELGLGAIPQEKDRRAIDLLELSLFFLDRLAESQPPE
                     FFMTRARR"
BASE COUNT          294 a          308 c          346 g          334 t
ORIGIN      
        1 cgagatgccc tttggcgtgg aaataatttc taccttgggg tttctttttc gcatttcaat
       61 aataaatgcg gtatggtcag gttccagatg attaagaatc aaataatcaa tcttttcaaa
      121 ggaagaacct gccgaggcca actgggtttc aagctggtct atcgaaccag cccaatcctt
      181 ggccaggtcg atcagcgcgg tctttgtgcc ggtaaccaga tatgaattaa gggtaactcc
      241 atgggggata ggccaaattc cctcgaatcg ggtgtcggtg tgtacatctg cgtgaacagc
      301 ccatacctga gcggctattt tgtgtgcgtt catatccatc tcctttagaa ccctcagtat
      361 aaaccggctt gtaaacttaa ttcaacaatc ccatagtctc attttttaaa agctggtaca
      421 gtgcatgcat gattaccgct ctggcgccta tggcgaccct tactcacgaa gccctgcgcc
      481 ttcttatcca tcgttttggc gatccaaccg agtattactc ggagatgatt catgccccat
      541 cgtatgtctg tggaggggtt ttcgagaagt ggtatgtcag gacagcaccg gcccccgaca
      601 aaatggtctg gcaattaacc gggccgaccg cttcttccat ggctcaagcc gcggccttgt
      661 tgctgccgct tggcggcttg ggtatagatg tgaatatggg atgcagcgct cctgatatcg
      721 taaaaaccgg ctgtggaatc gcctggatga gtaaggaagg ggcggaggta cgcaccctta
      781 tatcctcaat tcgctcgtgt atagaccagt atctaactga taacccgggg caacaaagac
      841 ggcttggggt aaagctccgc cttggagaag atgaggacta tacgcgtctg ttggattttt
      901 gccgaatgct cgtagacgag ggggtggagc aacttaccct gcatccgcgc actcgtcggg
      961 aaaagtatgg ccggcctccc cgccacgggt acacagtccg tcttggtcag gacattggtg
     1021 ttcccgtgta tggtaatgga gctatatcga gcgcagggga agccgtgtct tttatggaaa
     1081 aatatccgtg ttcgggtttg atgatagggc gcgcggctat tacccgtcca tggatattca
     1141 ggcagattga gcttggcttg ggggcaatcc cccaggaaaa agaccgaagg gcaattgatc
     1201 tattggagct gtcgcttttc tttttggacc gacttgcgga aagccaaccg ccagagtttt
     1261 ttatgactcg cgcgcgcagg tt
//
LOCUS       DAOQXQ010000583         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_138906_length_1278_cov_3.747343, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000583 DAOQXQ010000000
VERSION     DAOQXQ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138906_length_1278_cov_3.747343"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKH81_08810"
     CDS             complement(<1..1017)
                     /locus_tag="PKH81_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98175.1"
                     /translation="MRNVCGYAFKNIWRSRVRSVALLTIITVGVAVLTLIAAGYEDMF
                     YSAAKESREQEGDISFVDHSNDAKRSLSWESYLLLKERLLSTGMVHKVQASAQISGII
                     GGVDRSAPCSGIAYEGGIPLGEEDLVPIKMGGALAKTLSLAPTDTVGALLYDCGMTGR
                     VEQTVVTEVLLKDRFYVELPLEAFIVREEVPRINSVYIWLSKPLLAPRLYKDSDSPEY
                     QHIVELCSQAVELEGFTLYALPNNNTNVARIVQIYRTNYQVVLVVVIVTLLLAFLNVL
                     TLSVYERQQELGTLRSLGTPLVHIQILIVLETVCIALIGWLIAVALSFTASMIIQAAG
                     GLVFA"
     gene            complement(1014..>1278)
                     /locus_tag="PKH81_08815"
     CDS             complement(1014..>1278)
                     /locus_tag="PKH81_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98176.1"
                     /translation="RPHQLSGGQRQRVAIARSLMNMPDLIFADEPTANLDHTVGKEIL
                     ELLVSLNRDMNVTIVFSTHDPSVMACARRVISLQDGKIVGDTQ"
BASE COUNT          379 a          325 c          259 g          315 t
ORIGIN      
        1 ggcaaacacc aaaccaccgg cagcctgtat gatcatactt gcagtaaatg acagagcaac
       61 ggcgattagc catccaataa gggcaataca tacagtttca aggactatca atatctgtat
      121 atgcacaagt ggcgtaccaa gagatctgag cgtccccaat tcctgttggc gctcatatac
      181 cgataaggtc aatacattta gaaaagccag caaaagggtt acaataacaa ctaccagaac
      241 cacttgataa tttgttcgat atatctgaac gattctcgcc acattggtat tattatttgg
      301 cagggcatac agggtaaaac cttcaagctc tacagcttgt gaacacaact ctacaatatg
      361 ttgatactca ggagaatctg aatctttata taatcgaggg gcaagcaatg gttttgagag
      421 ccagatatat accgagttta tccgcggaac ttcttccctc acgataaagg cttcaagggg
      481 aagctcaacg taaaaacgat cctttaacaa aacctctgtt acgaccgtct gctctactcg
      541 acccgtcatt ccacaatcat acagcagcgc tcccaccgta tctgtgggag caagggaaag
      601 cgttttggca agagctccgc ccatttttat cggaacaagg tcctcttcgc caaggggaat
      661 cccgccttca taagcgattc cactacaagg ggccgagcgg tcaacacctc cgatgattcc
      721 gctaatctgc gctgaagcct gaaccttgtg caccataccc gtagataaca gacgctcttt
      781 aagcaaaaga tatgactccc aggaaagact ccttttagca tcattggaat gatcgacaaa
      841 actaatatcg ccttcctgtt ctctgctttc tttggccgca ctataaaaca tatcctcata
      901 tccggctgcg attagggtta acactgctac ccctacggtt atgatagtca acagggcaac
      961 cgatctaacg cgagacctcc agatattctt aaaggcatac ccgcatacgt ttctcattgt
     1021 gtatccccga ctatttttcc gtcctgtaag gaaataaccc gccgcgcaca ggccataaca
     1081 gacggatcat gagtggaaaa cacaatcgtt acattcatat ctctgttaag agagacaagc
     1141 agttcaagaa tctcttttcc caccgtatga tcaagattcg cggtaggctc gtctgcaaaa
     1201 attaaatcgg gcatattcat caaagagcgc gcgatagcaa cacgttggcg ctgacctccg
     1261 gaaagttgat ggggtctt
//
LOCUS       DAOQXQ010000584         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_139889_length_1273_cov_2.852217, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000584 DAOQXQ010000000
VERSION     DAOQXQ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139889_length_1273_cov_2.852217"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKH81_08820"
     CDS             complement(<1..845)
                     /locus_tag="PKH81_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98177.1"
                     /translation="MKDFALSNEDDLLELETSFSLDELDDWHTSTGLVESAMSMIRRR
                     VREADTHIFVHMPEAREYREIDREGLASIPDDSIFIACLAMCETGIPLNTIFQDYVPD
                     DDMLRELLTTVYRGYYIIPVVHRFSLLAFIMLCAPTPESKIRLPDQSQKDFLAELTSR
                     LRINLYAASIADQRQRELLRLAEYPASLHARRSVADLSEHLLDDLKKELDFTCGVYYE
                     YEEYLEKLIPIAWTGTKKEPQVLQAGCGISGQVIERRRPLWVPDRSKHPSFAMMSEEK
                     FIEGS"
     gene            complement(914..1108)
                     /gene="rlmJ"
                     /locus_tag="PKH81_08825"
     CDS             complement(914..1108)
                     /gene="rlmJ"
                     /locus_tag="PKH81_08825"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNQ98178.1"
                     /translation="MLHVQCRVPAPEAEQGFGLKGSAMIVINPPWKLDEDITAFLPWL
                     GKHLAPGLDNPTSLTWLSPN"
BASE COUNT          338 a          265 c          315 g          355 t
ORIGIN      
        1 aaagagcctt cgataaattt ttcttcgctc atcatggcaa aagatggatg tttagatcta
       61 tcaggaaccc agagaggcct tcgcctttct atgacctgtc ctgatatgcc acatccagct
      121 tgaagaactt gaggctcttt ttttgtgcct gtccaggcaa tgggtatgag cttttccaga
      181 tattcttcgt attcgtaata tacaccacag gtaaaatcga gttccttttt tagatcatcc
      241 agaaggtgtt cgcttaaatc tgcgacagat cggcgagcat gtagtgatgc gggatattcc
      301 gcaaggcgaa gtagttcccg ttgacgctga tctgcaattg aagcggcata tagattgatt
      361 ctaagtcggc tcgtcaattc agccagaaag tctttttgcg attggtctgg aaggcgaatc
      421 ttggattcgg gagtgggtgc gcagagcatg ataaatgcaa gaaggctaaa gcggtgaaca
      481 accgggatta tgtaatagcc gcggtataca gttgtaagaa gctcccgtaa catatcgtcg
      541 tctggaacat agtcctgaaa aatggtgtta agcggtatac cggtttcgca catggcaaga
      601 caggctataa agatggaatc gtcggggatg ctggcaagtc cttcgcggtc aatttcccgg
      661 tattctcggg cttcgggcat atgaacaaag atatgcgtgt ctgcttcgcg gacccttctg
      721 cgaatcatgg acattgctga ttcaactagg cctgttgagg tgtgccaatc gtcaagttcg
      781 tccaacgaaa agctggtttc aagttcaaga aggtcatctt cattggaaag ggcaaaatct
      841 ttcatatatg gatagattgt atgacgctct agtaaaatgt gtcaataaaa atctatcgat
      901 atagactatc cccttaattt ggggatagcc aggtaagtga cgtcgggtta tccaatccag
      961 gggcaaggtg tttgccgagc caaggtaaaa aggccgtaat atcctcatcg agtttccatg
     1021 ggggattaat aacaatcatt gccgaaccct taagcccaaa gccttgttct gcctcgggtg
     1081 ctggcactct acattggacg tgcaagattg agggaaagcc ggagtaactg atgttaactg
     1141 ataggaactg tcatttcgag tttttcgcgg ttttgggtca aaattcggtt ttttccccga
     1201 actacgactg ttttcgcaaa tccaatcaga attttccggc aacgccgact ccaagaagtg
     1261 taattccagt cta
//
LOCUS       DAOQXQ010000585         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_140043_length_1272_cov_3.385374, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000585 DAOQXQ010000000
VERSION     DAOQXQ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140043_length_1272_cov_3.385374"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKH81_08830"
     CDS             <1..622
                     /locus_tag="PKH81_08830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNQ98179.1"
                     /translation="HKEALRAAGVPYIGHTEIFGALTGTADPLTMFQVGALRIFFLTR
                     HVSLRQACSMVTKERIIDYVKRCSSMLASLRLTTGTMAIAGLNPHCGDGGLFGQEEIE
                     EIQPAVKYLQQQGIAVEGPVSADSVFHLAHQGRYSSVLSLYHDQGHIAAKTLDFERTI
                     AVTGGMPILRTSVDHGTAMDIAGKNLASGVSMEEAIRIAAGYVQSI"
     gene            complement(628..>1272)
                     /locus_tag="PKH81_08835"
     CDS             complement(628..>1272)
                     /locus_tag="PKH81_08835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ98180.1"
                     /translation="KGDPVAWIIMSERTTALEHIRSDLVFMYVSVLLSLILLISFLIL
                     IRRDRLRLKTLSLTDSLTGIANRVSLFDFLSREIHKSVRYDFDLSLILFDIDHFKQIN
                     DSYGHAEGDRVLVKTVEAVRSEIRSSDFLARLGGEEFVVILSHCPVEAAITAAEKIRR
                     AVAQAKVCESSPVTISVGVASLQKEERADSLIARADQAMYRAKEAGRNCVRVSP"
BASE COUNT          355 a          322 c          302 g          293 t
ORIGIN      
        1 ccataaagag gcgctccgag ctgcgggagt accctatatt ggtcacaccg aaatattcgg
       61 cgctcttacg ggaactgccg acccgcttac catgtttcaa gtaggagccc tgcgtatttt
      121 tttcctgact cgtcatgtgt ccctgaggca agcctgctcc atggttacca aagagcgcat
      181 catcgattat gtaaaaaggt gttcgtcaat gcttgcctca ctcaggctta ccacaggaac
      241 catggcaata gcgggattaa atccccattg cggcgatggt ggactttttg gacaggaaga
      301 aattgaagag atacagccag cggtgaaata tctgcaacaa cagggtattg cagtagaagg
      361 gccggtaagc gcagattcgg tttttcatct ggcacatcaa ggccgctatt caagtgtatt
      421 gtccctctat cacgatcagg gacacattgc cgccaagacc ctggattttg agcgaacaat
      481 cgcggtaacc gggggcatgc ccatcttgcg aacatcagtt gaccacggaa cggctatgga
      541 cattgcggga aaaaaccttg caagcggcgt aagtatggaa gaagcgatac gcattgccgc
      601 aggctatgtg caatcgatct aagtttttta aggactcacc cgaacgcagt tacggccagc
      661 ttcctttgcc cggtacattg cctgatccgc gcgagcaata agagaatcag cgcgctcttc
      721 cttttgtaaa gaagcgacac ccacactgat cgtaaccggg gaggactcgc aaacctttgc
      781 ttgtgcaact gcccgcctga ttttttcggc tgcagtgata gctgcctcta ctggacaatg
      841 cgaaagaatc accacaaatt cttcgccgcc aaggcgtgcc aaaaagtcag aagacctgat
      901 ctccgagcga actgcctcca cagtctttac caatacgcga tctccctccg catggccata
      961 ggaatcgtta atctgtttaa aatgatcgat gtcaaataaa ataagggaaa ggtcaaaatc
     1021 gtaacgaacc gatttgtgga tttctcgcga cagaaaatca aaaaggctta cgcggttcgc
     1081 aatccccgta agagaatcgg ttagcgaaag agtcttaagc ctgagccggt cccgacgaat
     1141 aagaatcaaa aaacttatca acagtatcag ggaaagcaac acacttacgt acataaatac
     1201 aagatcgctt ctgatatgtt caagggctgt agttcgctcg ctcataataa tccaggctac
     1261 cggatcgccc tt
//
LOCUS       DAOQXQ010000586         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_140623_length_1269_cov_2.826194, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000586 DAOQXQ010000000
VERSION     DAOQXQ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140623_length_1269_cov_2.826194"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..352
                     /locus_tag="PKH81_08840"
     CDS             65..352
                     /locus_tag="PKH81_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98181.1"
                     /translation="MCWKCGKAIIAEHPVGRTLTCETCGQDIRSCKNCAFYSPQSFHE
                     CAESVEDPVRDKERANFCDLFRINPSFTVDHDLTSSKDTSSTRAFNSLFGD"
     gene            364..1167
                     /gene="murI"
                     /locus_tag="PKH81_08845"
     CDS             364..1167
                     /gene="murI"
                     /locus_tag="PKH81_08845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNQ98182.1"
                     /translation="MSEAASSSYQYAFLDSGTGGLPYLRELARHADSARAVYVADTAH
                     FPYGEKTRDDVIHYAVDTTARLIERFSPSVVIVACNTISVAALSSLRQRFPVPFVGTV
                     PAIKQAALKSKNRRIGLLATERTVRDPYTDDLIEQWASDCTIVKIGDSRLISRIEGDL
                     ITASTEERIKAVGPSIDRFVSEGCDTIVLACTHFLHVSEEIALAAGEGVAIIDSREGV
                     VHQALRLSPPSDSASAARAFFYTGGAACAREDQYKSYASLFTCSWGGLL"
BASE COUNT          268 a          322 c          327 g          352 t
ORIGIN      
        1 ccggtaaaca taccactact gtaggttatt gcgcgttttt ggtcaaatgg gttatcatgt
       61 gcacatgtgc tggaaatgcg gcaaggccat aatcgcagaa catcccgttg gcaggacgct
      121 tacctgcgag acctgcggtc aggatattcg ttcgtgcaaa aattgcgcgt tttactctcc
      181 gcaatccttt catgagtgtg cagaaagcgt ggaagaccct gttcgggaca aggagcgcgc
      241 taatttttgc gatctgttcc ggattaaccc gtcgtttacc gttgaccatg acctgacctc
      301 gagtaaggat acctcctcca ctcgtgcctt taacagcttg tttggcgatt agggagttat
      361 cttttgtccg aagccgcttc tagttcctat caatacgcat ttcttgattc tggcaccggg
      421 ggcttaccct atcttagaga attagctcga catgcagatt ccgcccgggc ggtctatgtt
      481 gccgataccg cgcattttcc ctatggggaa aaaactcgtg acgatgtgat tcactatgca
      541 gtagacacca ccgcccggtt gattgaacgc ttttcgccct ctgtggttat agttgcgtgc
      601 aatactatct cggtcgcagc cctcagttcc cttcgacagc ggttcccggt tccctttgtc
      661 ggtacggttc cggcgattaa acaggccgcg ctcaagtcta aaaacagacg cattgggctt
      721 ttggctacgg agcgtaccgt acgcgacccc tatacagacg acctgatcga gcaatgggcg
      781 agtgactgta ccattgtaaa aattggcgac tcgcgtctta tttcccgaat cgagggcgac
      841 ttgataaccg cctctaccga agagcgcatc aaggcggtcg gcccgtctat tgatcgtttt
      901 gtctcggagg ggtgcgatac tattgtatta gcctgtactc attttttgca tgtaagcgag
      961 gagattgccc ttgccgccgg ggaaggagtc gcgattatcg attcccgcga aggcgtcgtt
     1021 catcaagcgc ttcggctttc tcctccttca gattctgcca gtgctgctcg cgcttttttt
     1081 tatactggtg gagccgcttg tgctcgcgag gaccaataca agtcatacgc ctctttattt
     1141 acctgttcat ggggaggcct tctgtgattg aaggacttgt ccttggcggt accaacaatc
     1201 aattcgaggt ggaatgtccg gatactcttg tgcggatatg cgggataaag ggcaagatac
     1261 ttaaagaat
//
LOCUS       DAOQXQ010000587         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_140838_length_1268_cov_2.562242, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000587 DAOQXQ010000000
VERSION     DAOQXQ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140838_length_1268_cov_2.562242"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKH81_08850"
     CDS             complement(<1..446)
                     /locus_tag="PKH81_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98183.1"
                     /translation="MIRPKAIATLARALIVALTVVFLASCATAPGQISGSAFSTLGDG
                     ALCYLVFPVREHQGLARRIGGEGEENLARAIDRTDRLWAGLYPASGSAEANNSIRLVA
                     SGSYPKSARSLVFPSSKGWVPALENAHGPWYFRGSEGGSIPRSGLYV"
     gene            complement(433..>1268)
                     /locus_tag="PKH81_08855"
     CDS             complement(433..>1268)
                     /locus_tag="PKH81_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNQ98184.1"
                     /translation="LRSPSDGKSVHLESYPVYDESLRDSALEFKMESVQKAVSMGRSL
                     RYQFNLKTRQPLKAVELVTRNPEEKKVLLEMEESIREELNVKEVIFHDKEDDLVEYTA
                     KANFRVLGKELGASMKQAASAIEGLSSSEIQSILEGATLSIEVEGIHVELTEEKILIH
                     RIEKANLRVVNEGTLTVALDTEVTEELLLEGCIRDLVRGVQNLRKERGLEVTDRIQLR
                     VSASGEGTDVLKKAFDNFSEYLSSETLAASALWVASGTHKDPVTVDAGEYLWEISLDK
                     A"
BASE COUNT          285 a          357 c          313 g          313 t
ORIGIN      
        1 acatataagc cggaccttgg aatgctacca ccttctgaac cccggaaata ccaggggcca
       61 tgggcgttct ccaaggctgg aacccatccc ttggaagaag gaaacaccaa ggatcgggca
      121 cttttgggat acgagccgct tgccacaagc ctgatactat tgtttgcctc ggccgagccg
      181 gaagcgggat acaatcccgc ccaaagtcga tcagttcggt ctatagcccg tgcaagattc
      241 tcctcccctt ccccgccaat acgccgggca aggccctggt gttcccgtac gggaaacacc
      301 agatagcaaa gggcgccgtc tcccagggtg gaaaaggcac tcccgctgat ctggcctggc
      361 gcggtggcgc acgatgccag gaagaccacc gtaagcgcta cgatcagagc gcgggccagg
      421 gtggcaatag ccttaggcct tatcaagcga tatctcccac aggtactctc cggcgtccac
      481 ggtaaccggg tctttgtgcg taccactggc gacccacaga gcgcttgcgg caagggtttc
      541 gctcgacaga tactcgctaa aattatcaaa cgcttttttg agtacatcgg tgccctctcc
      601 agaagcgctg actcgcaatt gtatgcgatc ggtaacctca aggccacgtt cttttcgtaa
      661 attttgcaca ccgcgaacca aatcgcgaat gcagccttca agaaggagtt cttctgttac
      721 ctctgtgtcc agggcaacgg taagggttcc ctcgttaacc actctaaggt ttgccttctc
      781 tatgcgatga atcaatattt tttcttcggt aagctcaaca tgtatgcctt ctacttcaat
      841 acttaaggtt gccccttcca atatggattg aatctcgctt gagcttaagc cctcaatggc
      901 agaggctgcc tgtttcatgg aagcgccaag ctctttcccc aaaacgcgga agttggcctt
      961 ggcggtatac tcaaccaggt catcttcttt atcgtggaag ataacctcct taacgttgag
     1021 ctcctcgcgg atgctttctt ccatttccag taataccttt ttttcctcgg gatttcgggt
     1081 taccagctcc acagccttaa ggggttggcg agttttaaga ttaaactgat agcgaaggct
     1141 tcttcccatg gacacggcct tttgcacgct ttccatcttg aactcaagcg cgctgtcgcg
     1201 aaggctttcg tcgtacacag gataggattc aaggtgtact gactttccat ccgagggtga
     1261 gcggaggt
//
LOCUS       DAOQXQ010000588         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_141235_length_1266_cov_2.528489, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000588 DAOQXQ010000000
VERSION     DAOQXQ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141235_length_1266_cov_2.528489"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PKH81_08860"
     CDS             complement(<1..1132)
                     /locus_tag="PKH81_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98185.1"
                     /translation="MLTKEQIKHSKWYRQSGAGIPLIGGLVHMGVPRMHEYSGFRMPL
                     VGLIIKTGDAFSFYNYLDYALVEKEADSILHKVDGDKNWFRGLYSQLSETAFTAEAVG
                     RRMLEERVGSPEFKADYELFIRTAVKGWGSSLFLDILDPFEKKIFSFIFGDRADSLKP
                     RDLTVLTMPDERSYVQRQQKDLLAIYKVAAKDGLKAVDRLVHEHAVKYHWLKNDFERV
                     EYLGVEHYMVQLETMLADPGMIKDIETGLARFDEAQKEKAALLSKLGLGDAIKGKLDF
                     FNWMASFRDERKRLAQITNYYMYGAGEKVATAMGLDMSWMQYALPMELLPLLCGDEKV
                     KSAIQIRQDIGVLFHVNENDHQEIISGPSIREYYEALEQRVAS"
BASE COUNT          320 a          353 c          255 g          338 t
ORIGIN      
        1 acgaagcgac ccgctgttcg agagcttcgt aatattccct gatggacggg ccggaaatga
       61 tctcctgatg gtcattttca ttgacatgga acaaaactcc gatatcttgt ctgatttgga
      121 ttgctgattt gaccttctca tcaccgcaca acaacggcag aagttccata ggcaaggcgt
      181 attgcatcca gctcatatcc aagcccatag ccgtggccac cttctccccc gcgccgtaca
      241 tgtaataatt ggtgatttgc gccaaacgct tacgctcatc gcggaaagag gccatccagt
      301 tgaagaaatc caatttccct ttgatcgcat cccccaaacc cagtttagat aatagcgctg
      361 ctttttcctt ctgggcttca tcgaagcgcg ccaggccggt ctcgatatct ttaatcatgc
      421 cggggtcagc aagcatcgtc tccagctgta ccatgtaatg ttccacgcct aaatactcta
      481 ccctttcaaa atcgttcttg agccaatgat acttaactgc atgctcatga accaagcgat
      541 ccaccgcctt tagcccatcc ttggccgcca ctttgtatat ggccagcaga tccttttgtt
      601 gtctttggac atacgagcgt tcatcgggca tggtcagaac agtcagatcc cgtggtttga
      661 gactgtcggc ccggtctcca aaaataaagc tgaatatttt cttttcaaaa ggatcaagta
      721 tatccaaaaa caaagacgat ccccatcctt taaccgcggt tcgaatgaaa agttcatagt
      781 cagccttgaa ttcaggggac cctacccgct cttcgagcat cctccgcccc acagcttctg
      841 ccgtaaacgc agtttctgat aattgagagt acaaaccacg gaaccaattc ttatctccat
      901 ctaccttgtg cagtatcgaa tccgcctcct tctccaccaa cgcataatca aggtagttat
      961 agaaggaaaa agcatcgcct gtcttgatta tcaggccgac caagggcatc ctaaaccccg
     1021 agtactcatg catgcgcggc acgcccatgt gcaccaagcc accgatgagg ggtattcccg
     1081 caccggactg cctataccat ttgctgtgtt tgatttgttc cttggttaac atatctaatc
     1141 aattacgaat tacgaattaa aaattacgat ctttgtcatt tcgagtgcta atcgagaagt
     1201 tcctttcggg gctatcgggc ttaagggatc tctccacctt ggtcgagatg acaatacccc
     1261 ccttct
//
LOCUS       DAOQXQ010000589         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_141584_length_1264_cov_3.185277, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000589 DAOQXQ010000000
VERSION     DAOQXQ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141584_length_1264_cov_3.185277"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKH81_08865"
     CDS             complement(<1..511)
                     /locus_tag="PKH81_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98186.1"
                     /translation="MTILCAQDFTPISPRIVSPRLMGFGGAYSALEAGTDTLSTNPAA
                     LAFTQPRWSFARTAANVSGPLFDLPSAFKADDVTYELLDLVQENNGIYIAAEATGPLA
                     VSRVEKNVGFGFFNRTMLLTDVPSLTKATILVGEEVLLVGGYGLTVWEKGAHSLSVGL
                     QLKGFFQVFV"
     gene            complement(559..>1264)
                     /locus_tag="PKH81_08870"
     CDS             complement(559..>1264)
                     /locus_tag="PKH81_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98187.1"
                     /translation="NKWTKAGVVLALSCVAFSSCSDIFTTSTASALERDSPGVSSSAS
                     VSDLMDIANSNTGANPEVAKEILDILADKKTSDLTSLSIEDKTAILNLAGTAAIPTGT
                     ITALAQDITEEGADSNALVEEAIGRFDSSVNLTAIEVLLSDPETLESASEESLVFASA
                     AVLADVANEVGANVVMEIMADPSLMQDSDLSQDQKDQINLVINVLDVIDSRPAEDGQT
                     SLGDFTLTDLLRGNKT"
BASE COUNT          323 a          305 c          316 g          320 t
ORIGIN      
        1 tcacaaagac ttgaaaaaag cccttaagct gtaatcctac agacagtgaa tgtgcccctt
       61 tttcccagac tgttagcccg tatcctccaa ccaacaatac ctcctctcca accaaaatag
      121 ttgccttggt aagagagggc acatcagtaa ggagcatcgt tcggttaaaa aaaccgaagc
      181 cgacgttttt ttcaacgcga gatacagcca atggtcccgt tgcttcggcg gcgatatata
      241 taccattgtt ttcctgtacc agatcaagca gttcgtaggt tacgtcatca gccttaaatg
      301 ccgaagggag atcaaacaag gggcccgata cattggccgc tgttcgtgca aatgaccatc
      361 ggggttgtgt aaaggcaagg gctgcgggat tggtggagag ggtatctgta cccgcttcca
      421 gagccgagta cgcgcctccg aatcccataa gccgcggaga gacaatacga ggtgatatcg
      481 gagtaaagtc ttgagcacac aggatcgtca tgctcagcgc gtatagcgcg cacagtagaa
      541 gtgtcgtcgc cctgtatctc atgttttatt acctcgcagt aaatctgtaa gggtaaagtc
      601 tcccaagctg gtttgtccgt cctctgcggg acgtgaatca ataacatcca gtacattgat
      661 aacaagattg atttgatctt tttgatcttg actgagatct gaatcttgca tgagcgacgg
      721 atcggccatt atttccataa caacatttgc tccaacctcg ttcgctacat ctgcaaggac
      781 tgcggcggaa gcgaatacaa gggattcttc gctggcggat tccagggttt ccggatcgct
      841 caggagaacc tctatcgcgg taagatttac cgatgaatca aatctcccaa tcgcttcttc
      901 caccagggca ttactgtctg caccttcctc ggtaatgtct tgagcaaggg ctgtaatcgt
      961 tccggttggg attgcggctg tgccagccag attaaggatg gctgttttgt cctcgattga
     1021 gagggaggta agatcggatg tttttttgtc ggccaatatg tccagaattt ctttagccac
     1081 ctcaggatta gccccggtgt ttgaattggc tatatccatc aggtcggaca cagaagcgga
     1141 agatgacacc ccgggtgaat cacgttcaag ggcagacgcc gtcgatgtgg taaaaatatc
     1201 gctgcatgat gaaaaagcaa cacaggaaag ggccagtacg acgccggctt ttgtccactt
     1261 gttc
//
LOCUS       DAOQXQ010000590         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_141866_length_1263_cov_2.113411, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000590 DAOQXQ010000000
VERSION     DAOQXQ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141866_length_1263_cov_2.113411"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1263
                     /locus_tag="PKH81_08875"
     CDS             <1..>1263
                     /locus_tag="PKH81_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ98188.1"
                     /translation="VKNSEIILKDAKKNGTTSDIRIPRTQNGSILVKWPKKEFKDYQT
                     LSAWSLIGYNRHEAAFVKNLKVMSEAGLFAYYEEEQTPYDLYSNAQYIREMLSEGENV
                     QDGITFDTYCAYRRDYLEKADAFLNGPYEKAILADNSGDAELEDYITELFAITRTQHA
                     ELLAMRETVAHTAKGAFCIIGVDATSMTDLGLTTFQERFPNVGIHATVANMLLSEEFL
                     DDTPIAITIIIALILSLSLGFFIKQLDTKKSMLAGAGTLVISVVVLLIFFIITRRYIG
                     VVVPFSSVTATFLSLSVFTFLTTVREKSFLRSAFSRYLSPAVINEIINDPSKLNLGGE
                     KRQMTAIFTDIRGFSGISEQMDPADLVNLLNLYLTEMSNIVLEHRGTIDKYEGDAIIA
                     FFGAPISMEDHATQACRTAICMKKAEVTL"
BASE COUNT          334 a          333 c          297 g          299 t
ORIGIN      
        1 gtaaaaaact ccgaaatcat ccttaaggac gcgaaaaaaa acggcaccac gagcgacatt
       61 cgcatccccc gcactcaaaa cgggtcgatc ctggttaagt ggcccaagaa agagtttaaa
      121 gactaccaaa ccctttctgc ctggagctta atcggctata accgccatga agccgccttt
      181 gttaaaaacc ttaaggttat gagcgaagcg ggcttatttg cctattacga agaagagcaa
      241 accccgtacg atttatattc caacgcgcaa tatattcggg agatgcttag cgaaggagag
      301 aacgtccagg acggaataac ctttgatacc tactgcgcct atcgcaggga ctatcttgaa
      361 aaggcggacg cgtttttaaa cggaccgtat gaaaaggcga tccttgccga caacagcggg
      421 gacgcggagc tggaggacta cataacggag ctctttgcca taacccgcac gcaacatgcc
      481 gagttactgg ccatgaggga aaccgtagcg cacacagcca aaggggcgtt ttgcataatc
      541 ggggttgatg ctaccagcat gaccgacttg ggccttacca cctttcagga gcggttcccc
      601 aacgttggca tacacgcgac ggttgcgaac atgctcttga gcgaggaatt ccttgatgac
      661 acgccaatcg caataaccat catcattgcc ctgatactgt cgctcagcct gggctttttt
      721 atcaaacagc ttgatacaaa aaaatcaatg ctagcgggtg cgggaactct cgttatctcg
      781 gtagtggtct tacttatctt ctttataatc acccgtcgct atatcggggt tgtcgttccc
      841 tttagctcgg taaccgcaac cttcctgagc ctgtctgtct ttacgtttct taccacggtt
      901 cgcgaaaaat cctttctccg ctctgccttc tcgcgctatc tttcgccggc ggttattaac
      961 gagattatca acgatccttc caaactgaac ctgggcggag agaaacggca gatgacggct
     1021 atctttaccg acatccgcgg tttttccggt atctcggaac aaatggaccc ggcagacctt
     1081 gttaatcttc ttaaccttta cctgaccgag atgagtaaca ttgtactgga acaccgcggc
     1141 accatagata aatacgaggg agacgcaatt atcgccttct ttggtgcgcc catcagtatg
     1201 gaagatcatg caacccaggc atgccggacg gctatctgca tgaaaaaggc agaggtaacc
     1261 ctg
//
LOCUS       DAOQXQ010000591         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_142798_length_1258_cov_1.994181, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000591 DAOQXQ010000000
VERSION     DAOQXQ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142798_length_1258_cov_1.994181"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1258
                     /locus_tag="PKH81_08880"
     CDS             149..>1258
                     /locus_tag="PKH81_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98189.1"
                     /translation="MKTKQLPVLWFLVACTLPLLGILAINQFRWLQELQTRERNDKQE
                     KIASSVQNLSRYLRDELLFFPTLLRVRNRSGQDGKAAFAERYQIWQNYALSPQLMSNI
                     YLYDTRSKVAQEWIDGSFITVGCPPCIQTLVSDLTFDDVPETFFRHRNKSILLVFPLP
                     HTTRRTTFVFCVLDSEVILTQIIPRLAEDSIDLNHDFDFRIHDYGNTRTLYMSPGNRT
                     EEEYSRPDVDISLRSSIGSAYTLRPNPLLQQRIASKRITETELAFSTERIIPDRPDID
                     SVLKSFESIRIQILYRDGSLEQITRRTTRQNAAISFGLVILLAVVIITLAEATRKSRA
                     LAASQKTFIASVTHELKTPLAVISSASQNLADGIVA"
BASE COUNT          344 a          319 c          253 g          342 t
ORIGIN      
        1 cattgcggga caagccttaa gttgacgcat caaggaattc tgtggatcct tcgtcaatgg
       61 caagacaaat tgtaacgata gtgtaaaata agcgctcacc gccggtcaac ctttaaccct
      121 ttactaaaaa tgaagtacaa tcacttttgt gaaaaccaag caattgcccg tcctctggtt
      181 tttggtggcg tgcacgttac cgcttctggg tatcctggct ataaatcagt ttagatggct
      241 tcaggaattg caaacccgtg aacgaaacga taaacaggaa aagatagcgt cctctgttca
      301 aaatttgtcc cgctatcttc gggacgagct tcttttcttt ccaaccctgc tgcgagtgcg
      361 taaccgctcg ggccaggacg ggaaagcggc ctttgccgag cgatatcaaa tttggcaaaa
      421 ttatgccctg tcgcctcaac tcatgtccaa tatctattta tacgacactc ggtcaaaagt
      481 agcccaagag tggatcgatg gttcatttat taccgttgga tgcccgccgt gtatacaaac
      541 gctggtgagt gatcttacct tcgacgatgt cccggaaacc tttttccgtc atagaaacaa
      601 gtcaattctc ctggtatttc cccttcctca tactaccaga aggacgacct ttgtcttttg
      661 cgttttagat tctgaggtta tccttacaca gataatcccg cgtcttgcag aagatagtat
      721 tgatttaaat cacgattttg attttcgtat tcatgattat ggaaacaccc gaaccttgta
      781 catgtccccg ggtaatcgga cagaagaaga atattcccgt ccagacgtcg acatctcttt
      841 gcgaagttca ataggcagcg cctacacact cagaccaaac ccactcttgc aacaacgaat
      901 tgcctcaaaa cgaattaccg aaaccgaact tgcgttttct accgagcgta ttattccgga
      961 cagaccagac atagattcag ttcttaaaag ttttgaatct atcagaattc agatacttta
     1021 ccgcgacggg tctcttgaac agattacccg tagaaccacc cgacaaaacg cggccattag
     1081 ttttggcctg gtaatccttc ttgccgttgt gattatcacc cttgccgaag ctaccagaaa
     1141 gtcaagagca cttgccgcga gccaaaaaac gtttattgcc tctgtaacgc atgaactaaa
     1201 aacccctctg gcagtcatat cttccgcctc acaaaatctt gcagatggca tcgttgcg
//
LOCUS       DAOQXQ010000592         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_142970_length_1257_cov_2.061564, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000592 DAOQXQ010000000
VERSION     DAOQXQ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142970_length_1257_cov_2.061564"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="eno"
                     /locus_tag="PKH81_08885"
     CDS             <1..280
                     /gene="eno"
                     /locus_tag="PKH81_08885"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422107.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ98190.1"
                     /translation="LNQIGTVTETIDAVRMAQKAGYTAVMSHRSGETEDSFIADLSVA
                     LETGQIKTGSMSRSDRVAKYNQLMRIEDELGITARFAGREPFELFVTP"
     gene            complement(372..977)
                     /locus_tag="PKH81_08890"
     CDS             complement(372..977)
                     /locus_tag="PKH81_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNQ98191.1"
                     /translation="MRNEEEEEDEQENKAPAEIDPLAHKFLKTRQILLSGEINKALAE
                     RIIRQLLVLEADSDEPIKLFIDSPGGDADAGFAIFDMIRFIKAPVYTIGMGLVASAGS
                     IILLAAPKARRLALPNSHYLIHQPLSGIKGVATEIEIHALELEKMRVRINELIAEETG
                     MEVDRVARDTDRDFWMSAGESKEYGLVSKVITTRAELESLS"
     gene            complement(1072..>1257)
                     /locus_tag="PKH81_08895"
     CDS             complement(1072..>1257)
                     /locus_tag="PKH81_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNQ98192.1"
                     /translation="ARHLKVDPGVALTRTNAKFTRRFTYVEHAMKKAGLPLDKAHLGE
                     MDVFWDEAKQQERQKKG"
BASE COUNT          307 a          327 c          295 g          328 t
ORIGIN      
        1 gttaaaccaa atcggaacgg ttaccgagac tatagacgcg gtgcgtatgg cccaaaaagc
       61 cggttacacc gcagttatgt cccatcgctc gggcgaaacc gaagacagct ttatcgccga
      121 cctgtccgtg gcccttgaaa ccgggcagat aaaaaccggc tctatgagcc gctctgaccg
      181 ggtagccaaa tacaatcagc tgatgcgtat cgaggacgaa ctcggtatta ccgcgcgatt
      241 tgccggcagg gagccttttg agctttttgt taccccataa gggaatcaac atacaataac
      301 aacataaaaa aaaggctgtc cgacacggtt gtgttgggca gccttttgta ttaccagctc
      361 atggaaaaaa atcaggacag gctttcaagc tcagctcggg tagtaatcac cttggacaca
      421 aggccatatt cctttgactc gccagcgctc atccagaaat ctcgatcggt gtcccgggct
      481 acccgatcta cctccatacc ggtttcttcg gcaataagct cattgattct gacccgcatt
      541 ttttctagct caagggcatg aatttcaatc tcggtagcaa cacccttaat ccccgacaag
      601 ggttgatgaa taaggtaatg gctattggga agggctaaac gtcgggcttt gggagcggcg
      661 agcaggataa tcgagcctgc gcttgctacc agtcccatcc caatggtata caccggtgcc
      721 ttgataaagc gaatcatgtc aaatatagca aagccagcgt cggcatcccc gccaggagag
      781 tcgataaaga gcttgattgg ctcatctgaa tcggcttcca atacgagtaa ttgtctgata
      841 atcctttcgg ccagggcctt gtttatctcg cccgaaagca aaatttggcg tgtttttaag
      901 aatttatgtg ccagaggatc aatttccgcg ggagccttat tctcctgctc gtcttcttct
      961 tcctcttcgt tgcgcattgc acggcgtata tcggtatatc tggcttgttt cactcatctc
     1021 tccttatgtt gtatcttgaa tgtagcaaaa aaaacggcaa ccgtacaggg tttacccttt
     1081 tttctgccgt tcctgctgtt ttgcctcgtc ccaaaacaca tccatttccc ctaaatgggc
     1141 cttgtccaga ggaagtcccg cctttttcat cgcatgttcc acataggtaa agcgccgggt
     1201 aaatttggcg ttagtacggg ttagggctac ccccgggtct accttaagat gacgggc
//
LOCUS       DAOQXQ010000593         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_143607_length_1253_cov_3.535058, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000593 DAOQXQ010000000
VERSION     DAOQXQ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143607_length_1253_cov_3.535058"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKH81_08900"
     CDS             <1..243
                     /locus_tag="PKH81_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNQ98193.1"
                     /translation="FVKALDEKGMGGDVIIRQTGCMGLCQNEPTVEIVMKDMPNIIYG
                     RVDAKVAKQIVEKHILQKELLNEQILDRPAADILKK"
     gene            256..>1253
                     /locus_tag="PKH81_08905"
     CDS             256..>1253
                     /locus_tag="PKH81_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ98194.1"
                     /translation="MAYNHYILVCGGTGCESSNADGIYNNLISEAKAAGVLEKVQIVK
                     TGCFGFCEKGPIVKVLPEDSFYVEVKPEDAKEIINEQIVKGREVTRLLYNKERKSTES
                     VEEIQFYQKQLRIVLRNCGVINPENIEEYIARDGYLALEKALFDMTPQQIIDELKASG
                     LRGRGGAGFPTGLKWEGALKTQNDQKYVVCNADEGDPGAYMDRSTIEGDPHSVIEAMA
                     ICGKTIGANQGFVYIRAEYPLAIDRLKIAMNQAREAGLLGKNILGSGFDFDIEIRLGA
                     GAFVCGEETALLRSIEGHRGMPTPKPPFPAQSGLWGKPTVINNVETWANVPVILTKG"
BASE COUNT          353 a          324 c          316 g          260 t
ORIGIN      
        1 tttgtcaaag cgcttgacga aaagggtatg ggtggagacg tgattatccg ccaaaccgga
       61 tgcatgggcc tttgccaaaa cgagccgacc gtagaaatcg tcatgaaaga catgccgaac
      121 atcatctatg gccgagtaga cgcaaaagtt gcaaaacaga tcgtggagaa gcacatcctt
      181 cagaaggaac ttcttaacga gcagatcctc gaccgccctg cggccgacat tctgaaaaaa
      241 taaggagaga agtacatggc atataaccac tacattctcg tctgcggcgg aactggatgc
      301 gaatccagca atgccgacgg gatttacaac aacctgatca gcgaagccaa ggcagccggc
      361 gttctggaaa aagtccagat cgtcaagacc ggttgtttcg gtttttgcga aaaaggaccg
      421 atcgtcaagg ttctacccga agattctttt tacgtcgagg taaagcccga ggacgccaaa
      481 gaaatcatca acgagcagat tgttaaggga cgcgaagtta ctcgactcct ttacaataaa
      541 gagcgaaagt ctacagaaag cgtagaagaa attcagttct accagaagca acttcgcatc
      601 gttttgcgaa actgcggtgt tatcaatccg gagaacatag aagaatacat cgctcgcgat
      661 ggatacctcg cgcttgaaaa agccctgttt gacatgaccc ctcaacaaat catcgacgag
      721 ctcaaggcct ccggtcttcg tggtcgcggt ggtgccgggt tccccaccgg gctcaagtgg
      781 gaaggtgctc ttaaaaccca gaatgatcaa aaatatgttg tctgtaacgc cgacgaggga
      841 gaccccggag catatatgga ccgctcaacc atcgaaggag acccccactc cgttatcgag
      901 gccatggcga tctgcggaaa aactattgga gccaatcagg gctttgtgta tatccgcgct
      961 gaataccccc ttgccataga ccgactcaag atagcgatga accaggcacg cgaagcgggt
     1021 ctccttggaa agaacattct tggctccggc tttgactttg acatcgaaat ccgccttggc
     1081 gcgggagcct ttgtgtgcgg cgaggaaact gcccttctca ggtcaatcga aggtcatcgc
     1141 ggtatgccaa cccccaaacc tcccttccct gctcagtccg gtctctgggg caaacccact
     1201 gtaatcaata acgttgaaac atgggcaaac gttccggtaa tccttaccaa ggg
//
LOCUS       DAOQXQ010000594         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_143613_length_1253_cov_3.434057, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000594 DAOQXQ010000000
VERSION     DAOQXQ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143613_length_1253_cov_3.434057"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKH81_08910"
     CDS             complement(<1..590)
                     /locus_tag="PKH81_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98195.1"
                     /translation="MDKKLSVVEVVKEGIGIGMVNMVPILVNTLLWILTIWIPYLNIG
                     TTIGMVVGIVTKASKGESISMTEIFNPQYRKYMGEFFLTSGFVSMGVGAALVFLIIPG
                     IVLAIAWSLALLLAVDKEKNPMEAISLSNKITYGNKWAIFLSEFVVLLGAGILSSVFS
                     RIPGIGGLLVLAVMIVAIFAGIGIRAYIYKTLTANV"
     gene            706..>1253
                     /locus_tag="PKH81_08915"
     CDS             706..>1253
                     /locus_tag="PKH81_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98196.1"
                     /translation="METLLKVQGLTKKYGPHTAVAGVTFSVNRGEIFGLIGANGAGKS
                     TTIECIVGTKKPDKGTITILGVDGIAQGKSICGRIGFQFQETPYQDRILVKEACRVTS
                     SLYTNPLPWHEVLTRVGLLEKSNMEVSKLSGGERQRLSVALALIPNPELVFLDELTTG
                     LDPKARRSMWKMIEELRDRGVS"
BASE COUNT          392 a          298 c          256 g          307 t
ORIGIN      
        1 tatacgttag ctgtcaatgt cttataaatg taggctcgaa tgccaatccc agcaaagata
       61 gccactatca ttacagcaag aacgagtaaa ccgccaatgc caggaatcct ggagaataca
      121 ctgcttaaaa tacctgcgcc aagcaataca acgaattcag aaaggaagat agcccacttg
      181 tttccatagg ttattttatt gctcagggat atcgcttcca taggattctt ttctttatcc
      241 acagccaata acaaggctaa actccaggct atggcaagaa caattccagg aataataaga
      301 aaaacaagag ccgccccaac acccattgaa acaaaaccgc ttgtcaaaaa gaactcaccc
      361 atgtatttac gatactgagg attaaaaatc tcggtcattg aaatcgactc gcccttactg
      421 gcctttgtaa caataccaac aaccatacca atagtcgttc cgatattcaa atacggaatc
      481 cagatagtca gtatccaaag cagggtgttc actaaaatgg gaaccatgtt caccattcca
      541 ataccaatgc cctctttaac gacctctaca acagacaatt ttttatccat aacgaatcct
      601 ccttaggaaa ctatacatat agtttatcac gagcgctctc ttgtccgcaa ttttttttct
      661 tttttttctt gactctcccg ttacggcaga ctgtacgctt gaattatgga aacactacta
      721 aaggttcaag gccttacaaa aaagtacgga ccgcacactg cggtcgcagg ggttactttc
      781 tccgtgaaca ggggagagat tttcggttta atcggtgcaa atggcgcggg taaatccaca
      841 accatcgaat gcatcgtagg aacaaaaaag cctgataagg ggaccatcac aatccttggt
      901 gtggacggaa tcgcacaggg taaatccatt tgtggccgga tcggttttca attccaggaa
      961 acgccgtatc aggacagaat ccttgttaag gaagcctgca gggtaacatc atcactttat
     1021 acaaatccac tgccctggca cgaagtactc acccgggtcg gacttctgga gaaaagcaac
     1081 atggaggtat ccaagctctc ggggggagaa agacaaagac tttctgttgc attggccctt
     1141 atccccaatc cggagcttgt ctttcttgat gagcttacaa cagggctgga tccaaaagcc
     1201 agacgatcca tgtggaagat gatagaagaa ctgagggaca ggggggtatc cat
//
LOCUS       DAOQXQ010000595         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_144202_length_1250_cov_3.242678, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000595 DAOQXQ010000000
VERSION     DAOQXQ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144202_length_1250_cov_3.242678"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PKH81_08920"
     CDS             <1..>1250
                     /locus_tag="PKH81_08920"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ98197.1"
                     /translation="VTNIMELHAPIGTPTIWYAKLQYISFTYLPIVWLFFCLQYTGWI
                     SWTRKWLIAIAVLAPLPLLVLTFTNEYHGFLWRVLTFAQAGGLSVLRAEYGPLYWFLF
                     TYSWLFIGTGAVMVVRSYVTGQGLYYRQSLWMILGLFFPAVTNLLNLTRVFPSMGKDF
                     TPMGYAVSGLCFLAAMYIHRLFWIMPASRGVILQEIDIGILVVDRNGWIVDHNQMIDQ
                     LIGITEIAVGKKAVDMVDVTEFFSACGIRELASDEKPSSGMLEWKGRMISWSRQAYQG
                     RRYGFIFTAKDITEVISLQAEIVKLKEEFINREKLASIGRLTATLAHEINNPLAYITA
                     DLRSLQRILALPEQNRLSTDITDTVSSLSSGLQRIEGVVTSLLAFSRKGSIETVMGEY
                     DMHRGISITLEFMRFELHDSVQVT"
BASE COUNT          290 a          300 c          300 g          360 t
ORIGIN      
        1 ttgttacgaa tatcatggaa cttcatgctc ccataggcac ccctaccatc tggtacgcaa
       61 agcttcaata catatcattt acctatcttc ccattgtgtg gctgtttttt tgtttacaat
      121 acaccggctg gatcagctgg acccgaaaat ggcttatagc cattgcagtt cttgcccccc
      181 tgccgcttct tgtgttgacc tttaccaacg agtatcacgg ttttctttgg agagtactaa
      241 cctttgctca agcgggggga ctttcggtat tacgggctga gtatggtccg ctttattggt
      301 ttctctttac ctactcatgg ctctttatcg gaaccggtgc agttatggtg gttcgctcgt
      361 atgtaaccgg acagggactt tattatcgcc aatccctgtg gatgatcctt ggcctgtttt
      421 ttccggcagt aacaaatcta cttaatctaa ccagggtttt cccgtccatg ggcaaagact
      481 ttaccccaat ggggtacgcg gtttcgggcc tgtgtttttt agccgcgatg tatattcacc
      541 gtcttttttg gatcatgccc gcctcccgcg gggttatcct gcaggaaata gacatcggta
      601 ttctggtggt cgatcgaaat ggatggatcg ttgatcataa ccagatgatc gaccagttaa
      661 tcggcataac agaaatcgcc gtggggaaaa aagcggtgga catggttgat gttaccgaat
      721 tctttagcgc atgcggaatc cgggagttag cctctgacga aaagccgtcc tccgggatgc
      781 ttgaatggaa aggaagaatg atttcctggt cgcgtcaggc ctaccagggc aggcgatatg
      841 gatttatctt tacggctaag gatattaccg aggttatttc gttacaggca gagatagtca
      901 aactcaaaga agaatttata aatcgggaga agcttgcttc aatcggtcgg ttaacggcaa
      961 cccttgccca cgagattaat aatcctcttg cgtatatcac cgcagatctt cgctccctcc
     1021 agcggatact ggctctgcca gaacaaaacc gtctgagcac agacattacc gacaccgttt
     1081 cctcactatc ctccgggctc cagcgcatcg aaggggtggt cacgtcactc cttgcgtttt
     1141 cccgaaaagg aagcatcgaa acagttatgg gtgaatatga catgcatcgt ggcatttcca
     1201 ttacgcttga gtttatgcgc tttgaacttc atgatagtgt tcaggtgacg
//
LOCUS       DAOQXQ010000596         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_144564_length_1248_cov_3.242246, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000596 DAOQXQ010000000
VERSION     DAOQXQ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144564_length_1248_cov_3.242246"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1248
                     /gene="secA"
                     /locus_tag="PKH81_08925"
     CDS             <1..>1248
                     /gene="secA"
                     /locus_tag="PKH81_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695026.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ98198.1"
                     /translation="RISALLTKKGVRHEVLNAKNHAREALIIAEAGAKGSVTIATNMA
                     GRGTDIKLGGNPEFRARRRAGTEASEEQYQKILAEEYEKWKADYLEVKSLGGLYVIGT
                     ERHESRRIDNQLRGRSGRQGDPGRSRFFISLDDDLMRMFGGENLKSIMSKIGMASGEP
                     IWHPWLNKSIERAQKKVEERNFEIRKHLLEYDDVLNEQRTFIYKQRSDILADQKLSER
                     ILNTAEEFLDELCDEYSHAQRRGKDEALASFVTALKERFGYALTEEEKSAMLLSPDAG
                     KEAVLASLRTDITSKENLAGTEGLNAFIRFQYLQAIDKKWLDHLENLESLREAVYLRS
                     YGQKNPLTEYKLEGFDIFYSMLDDIRMEIASRVFLVKVRTEGNEPVSRHTRQVSINAQ
                     HQGLGSFAGGRSGPNATSNPENV"
BASE COUNT          383 a          275 c          307 g          283 t
ORIGIN      
        1 aagaatctct gcgctcttga ctaaaaaagg tgtacgccat gaagtcttaa acgccaagaa
       61 ccatgcacgg gaagccctta ttattgccga agcgggcgct aagggttcgg taaccatagc
      121 aacaaacatg gcaggacgcg gaacagatat taaattgggc ggcaatccgg agttccgcgc
      181 gcgaagaaga gctggaaccg aggcctccga agagcagtat caaaagatcc ttgccgaaga
      241 atatgaaaaa tggaaagccg attatcttga agtaaaaagc cttggtggct tatacgtaat
      301 tggaaccgaa cgacatgaaa gccgccgtat agacaatcag ctacgcggtc gctctggccg
      361 acagggagat cccggacgat ctcgcttttt tatatcgctt gacgatgatt tgatgcggat
      421 gtttggcgga gaaaatctta aatcaattat gtccaagatc ggaatggcca gtggagaacc
      481 catctggcat ccctggctca ataaaagcat tgaacgtgcc caaaagaagg tagaagagcg
      541 taactttgaa attcgcaagc atcttcttga atatgatgat gtgctgaatg aacagagaac
      601 ctttatttac aaacaacgaa gcgatattct tgcagaccaa aagttaagcg aacgtattct
      661 taatactgcc gaggaattcc ttgatgaact gtgcgatgag tattctcatg cacaacgccg
      721 cggaaaggat gaagcccttg cctcttttgt gacggcactg aaggaacgat ttggatatgc
      781 gttaaccgaa gaagaaaaaa gcgcaatgct attaagtcct gatgccggaa aggaagcagt
      841 actcgcctcg cttcgcacgg atattaccag caaggaaaat cttgcaggca cagaagggct
      901 taatgccttt atacgattcc agtatctaca ggcaattgac aaaaaatggc ttgatcacct
      961 tgagaatctt gaatcccttc gcgaagcggt atacctcagg tcctatggac aaaagaatcc
     1021 ccttaccgaa tataaactgg aaggctttga tatattctat tcaatgcttg acgatatccg
     1081 gatggagatt gcgagcaggg tattcctggt taaggttcgc accgaaggga atgagccggt
     1141 atccagacac acccgacaag tgtccattaa cgcccaacat cagggcttgg gatcctttgc
     1201 tggtggacgt tcaggtccca acgctacgag taaccccgag aatgtaca
//
LOCUS       DAOQXQ010000597         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_145420_length_1244_cov_2.460892, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000597 DAOQXQ010000000
VERSION     DAOQXQ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145420_length_1244_cov_2.460892"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..748
                     /gene="trpA"
                     /locus_tag="PKH81_08930"
     CDS             8..748
                     /gene="trpA"
                     /locus_tag="PKH81_08930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNQ98199.1"
                     /translation="MAHLVAGFPSEENFFAAASALAQGGVSFFEIQFPFSDPSADGPA
                     IQGACAASLAAGFTVARGFQMVRSLRERYPDIPVYIMTYGNLAYKCGIDSFVEQSAAA
                     GVSGLIIPDLPFDSDEGLGEACARHGLLSVPVAAPSMSDARKRELLSHKAPLVYAALR
                     SGITGSKTSIDEATLSFLASLSGNGTRVLGGFGIQDGEQSRALSPHVHAVVAGSAFVN
                     LISANASRGSASIQDALTAKARELCGLA"
     gene            818..>1244
                     /locus_tag="PKH81_08935"
     CDS             818..>1244
                     /locus_tag="PKH81_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ98200.1"
                     /translation="MKLKKIAQTIHIILSIFMVLILFSRLGMVIWGTEVYPYRLVNEI
                     PAVRTFALMVIPLIVLVLLLILLSVSGVMDSRERLHISLSQLYQSVFHGLSTGLLVLD
                     GQGRIRYINTTGCRLLGWESGSPPQLMHYSELVDPLLVPV"
BASE COUNT          219 a          336 c          332 g          357 t
ORIGIN      
        1 caggcttatg gcccatttgg ttgcgggttt tccatcggaa gagaattttt ttgccgcagc
       61 cagcgccctt gcccaaggcg gtgtttcgtt ctttgaaatt cagttcccct ttagcgatcc
      121 aagtgcggac gggccggcta tccagggcgc atgcgcagcc tcccttgccg cgggttttac
      181 cgtagccagg gggttccaga tggttcgctc tctccgcgag cgctaccccg atatcccggt
      241 atatattatg acctacggga accttgcgta taagtgcggt atagactctt ttgtcgagca
      301 aagcgcggca gccggggttt ccggcttgat tatcccggat ttacccttcg actcggacga
      361 gggccttggc gaagcctgtg ctcgccatgg gcttctctct gttccggtcg cagctccttc
      421 catgagcgac gctcgtaagc gggagctctt gtcccataag gcaccactgg tatacgcagc
      481 gctcagaagc ggtattaccg gcagtaaaac aagcatagat gaggcaaccc tttcgttttt
      541 ggcgtcttta agtggaaacg gtacccgggt cttgggcggt tttggtattc aggacgggga
      601 gcaatctcgc gcactttctc cgcatgttca tgccgtagtg gccggttcag cctttgttaa
      661 tctgatatct gccaatgcaa gccgtggatc ggccagtatt caggatgcgc ttactgcaaa
      721 ggcacgggag ctctgcggct tggcatagca ggtccaggca gtttgatccg cccgtttgct
      781 ttttaccctg tattctgcct atactggaag tctaaagatg aagctcaaaa agatcgcgca
      841 aactattcac attattctct cgatctttat ggtcctgatt cttttttccc gtttgggtat
      901 ggtcatttgg ggcaccgagg tgtatcccta tcgccttgtc aatgaaattc ctgccgttcg
      961 cacttttgcc ctcatggtca tcccgcttat tgtcctggtt cttcttctta ttttactgtc
     1021 ggtaagcggg gttatggatt cccgcgaacg gttacatatc agtctttccc agctctatca
     1081 gtctgtgttc cacggccttt ctaccgggct tttggtcctt gatgggcagg gccgcattcg
     1141 gtatatcaat accaccggct gtcggctttt gggctgggag agcggctcgc ctccacaatt
     1201 gatgcactac agcgagctgg ttgatccgct cctggttccc gtgg
//
LOCUS       DAOQXQ010000598         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_145552_length_1243_cov_3.695286, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000598 DAOQXQ010000000
VERSION     DAOQXQ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145552_length_1243_cov_3.695286"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKH81_08940"
     CDS             complement(<1..300)
                     /locus_tag="PKH81_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HNQ98201.1"
                     /translation="MRRISSNIQHDDIQYSLRRQESRLSDANNSIASQRKNNRLRDDP
                     LAAGHTIRYQSYLSRLDRFDRNVKTVQDQFKITEGYVNQSLQVMQRVRELAVTGAH"
     gene            complement(311..>1243)
                     /locus_tag="PKH81_08945"
     CDS             complement(311..>1243)
                     /locus_tag="PKH81_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNQ98202.1"
                     /translation="RDGTGANGKTGLDFFVEQDFINNIAGNFDQNGDGEYDSSYIYRI
                     TGANQLSAREQIGLSGTLTLSAAEGNITVPYSSTDMVADVVARINNSGSEVVAHLDRD
                     GRLVLKGTTSANKENPDFVIRHVEDSGRFLAGYAGILSAQGEEGAFRWNQADAVTALS
                     GGELSWSVSPIAHPAGWMEINQVLSRDPLGIAAGFPGSDGQVNPGDNRAALAIAAIRN
                     SPVMVGNTRTFDDFFADAVTAIGLKGEQASRANETQLAILKELKGMRDSISGVNIDEE
                     LADIIKFQHGYNAAAKFISTINEMFDTVINRMGV"
BASE COUNT          318 a          310 c          282 g          333 t
ORIGIN      
        1 atgagcgccg gtaacggcaa gctctctaac acgctgcatg acctgtaagg actgattgac
       61 atatccttcg gtaatcttga attgatcttg cacggtttta acgttgcgat caaagcgatc
      121 gagccttgaa agataggact gatagcgaat ggtatgtcca gcagccaatg gatcgtctcg
      181 aaggcgattg ttttttcttt ggctcgcaat tgagttgttt gcatcgctca gtcgagactc
      241 ttgcctgcga agcgaatact gtatgtcatc gtgctgaatg ttgctgctta tacgtctcat
      301 cagttacccc ttatacgccc atacggttta tcaccgtatc gaacatctcg ttaatcgttg
      361 aaataaactt ggccgccgcg ttatatccat gttgaaactt gataatgtcc gcaagctctt
      421 cgtcaatatt tactccggaa atggaatccc tcattccctt taattctttc agaatggcca
      481 actgagtttc atttgcccgt gaagcctgct caccctttag accaatagca gttactgcgt
      541 ctgcaaaaaa atcgtcaaag gttctggtgt ttccaaccat aaccggagag ttgcgaatag
      601 cagcaatggc tagggccgca cgattatcgc cgggattaac ctgtccatca gagcccggga
      661 atccagcagc tattccaagg gggtcgcggg acagcacctg gtttatttcc atccatccgg
      721 caggatgggc gatgggagac acagaccagg aaagttctcc gccagagagt gcagttaccg
      781 catcagcttg attccaacgg aatgcccctt cttcaccttg agcgctcagg atgcccgcat
      841 agccggccaa aaaacgacct gaatcttcga catgtctgat aacaaaatca gggttttctt
      901 tattagccga agtagttcct ttaagcacaa gacgaccatc gcgatctaga tgcgctacaa
      961 cttcagatcc ggaattattg atacgtgcaa caacatcggc gaccatatcc gtagaagaat
     1021 agggaacggt aatgttcccc tcagctgcgg acagggtaag ggttcctgat agaccaatct
     1081 gctcccgtgc ggacagctgg tttgcaccgg ttattcgata gatataactt gaatcgtatt
     1141 cgccatcacc gttttgatca aagtttccag cgatgttatt gataaaatct tgctctacaa
     1201 aaaagtccag gccagttttg ccatttgctc cggttccatc acg
//
LOCUS       DAOQXQ010000599         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_145790_length_1242_cov_2.592249, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000599 DAOQXQ010000000
VERSION     DAOQXQ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145790_length_1242_cov_2.592249"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..>1242)
                     /gene="mtaB"
                     /locus_tag="PKH81_08950"
     CDS             complement(25..>1242)
                     /gene="mtaB"
                     /locus_tag="PKH81_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNQ98203.1"
                     /translation="EASMGMDPALCIVNTCTVTGKAEQKARRLIRLLLRSWPSTTVLV
                     TGCYAEVEEHAISAIDPRVLVLPGKRKNELASLPSFINTALLTRGNADIATIVHQFIQ
                     DTHHEAGAFRLSTDNFRFHSRASIKIQDGCNNRCAYCRICLARGASVSLPADEVISRI
                     KEIEQANWAEVVLTGVNLSQYRSGEGDFAQLLSSILAQTKHIAIRISSLYPDRVDSEL
                     VPLLNNPRIRPHFHFSVQSGSEKILSAMRRPYHNDQVLEAVHQLRLVKDDPFIACDII
                     TGFPGETDKDFDQTLEMCLKAHFAWIHAFPFSPRPGTEAWTMGNRVPERIAGDRVKVL
                     TDLAHANLEAYSRRWIGKTVKAVIEKSSGQHQGQALTENYLTVSLDELCPPRGTSVVV
                     ELIDMGKARLIPQ"
BASE COUNT          307 a          291 c          345 g          299 t
ORIGIN      
        1 attcaaacaa gagcaaagct caacttattg gggtatcagt cgtgcttttc ccatatcaat
       61 gagctcaaca actaccgatg tccctcttgg cgggcaaagc tcatcaaggg aaacggtaag
      121 ataattctcg gtaagtgcct gaccctggtg ctggccagag gatttttcga ttaccgcttt
      181 caccgtttta ccgatccatc gccgcgaata agcttcaaga ttagcatggg caagatcagt
      241 gagtacctta accctatctc ccgcaatgcg ctctggaact cggtttccca tagtccacgc
      301 ttcggtgcct ggacggggag aaaagggaaa ggcgtgtatc caggcgaagt gtgcctttag
      361 gcacatctct agggtttggt caaaatcctt gtcggtttcg ccgggaaagc cggtgattat
      421 atcgcaggcg ataaagggat cgtccttaac aagacgcagt tgatggacag cctcaagaac
      481 ctgatcgtta tggtacgggc gcctcatggc agaaagtatt ttttcgctac cggattgaac
      541 tgaaaaatga aaatgcggac gaatacgagg attattcagg agagggacga gctctgaatc
      601 gacgcggtcg gggtagagac tggatatcct gattgcaatg tgcttggttt gagcaaggat
      661 ggatgaaagg agttgggcaa aatcgccttc cccgctacgg tactgcgata aattgacccc
      721 agttaagaca acttctgccc agttggcctg ttcaatttcc ttaattcgcg aaataacctc
      781 atcggctgga agggaaaccg aggcgcccct tgcaaggcat attctgcaat acgcgcagcg
      841 attgttgcat ccatcctgga tctttatcga agctcgggaa tgaaaccgga aattatcggt
      901 ggaaagcctg aacgcccccg cttcatggtg agtgtcctgg ataaattgat ggacgatggt
      961 cgcaatgtcg gcgttgccac gggtaagtaa ggcagtgttt ataaaggagg ggaggctggc
     1021 aagctcgttt ttccgctttc ccggaagaac caggactcgg gggtcgatag cgctaatggc
     1081 gtgctcttca acttctgcgt aacagccggt aaccaagacg gttgtggagg gccatgaacg
     1141 gagtaacagc cgaatgagtc ttctggcctt ttgctcagct tttccggtga cggtacaggt
     1201 gttgacgatg cagagggcgg ggtccatccc catactcgcc tc
//
LOCUS       DAOQXQ010000600         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_146232_length_1240_cov_2.100422, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000600 DAOQXQ010000000
VERSION     DAOQXQ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146232_length_1240_cov_2.100422"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1189
                     /locus_tag="PKH81_08955"
     CDS             425..1189
                     /locus_tag="PKH81_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98204.1"
                     /translation="MRKVYTAIFMLLIAACFAIANISILSSQEKALKKIYISSIQGSG
                     VPEPLAKKARERIEFALYENFGKKYRVLTDDDVKVMFKKAQAIMATGCTAESCQTEIA
                     DAVDADEIIYGELSRDGTVLRLTTRNLLRDRKSLAITKKSMVVLSFIDEELDHYAAEA
                     AKKLIEPAYAIQKPAVKTYDGVSLAAVKLEEVKGLDIAVMRFTSDDATIQAMLTYLKE
                     LVEEWGSMRNFRNCSEASQRQCVGRVTTRVYNPLFW"
BASE COUNT          313 a          304 c          334 g          289 t
ORIGIN      
        1 ccccccggcc ccctctccca ttaagcttgg aagaaaggag agggggtgtc tcatgtttcg
       61 ggcttttccg cttagcggta atcgcgcagt agatctatta tcggtgctat cgcaagcaga
      121 ccagggacga gcgcttcgct atgctcgtgt tcgcggtaaa gatactcgag gagactgcgc
      181 ttggggttgt cgagagggcg taggctgggc gtttgtgaag cactgccctg gcaataattt
      241 tcgcaataat tataccctgt cgaaccattg caaaaatgtt gacatgcctc ccgatatccg
      301 ttatcgtact tcaaactcaa gccgcgcacg tcgcaccggg ccggcgcccc ctcgcgatta
      361 agaggggggt atgcctcgct cgcgcttgtg atagttccct tattatcgaa ggaagatgtg
      421 tgctatgaga aaagtgtata ccgcaatctt catgctttta atcgccgcat gctttgccat
      481 tgcgaacata tcgatacttt cctcccagga gaaggcgctc aagaagatct acatatcgtc
      541 catccaaggg agcggcgtcc cggagcctct ggcaaaaaag gcccgcgagc gcattgaatt
      601 cgctctctat gagaatttcg gcaaaaaata ccgggtgctg acggacgatg acgtcaaggt
      661 gatgttcaag aaagcacagg caataatggc caccggctgt accgccgaga gctgccagac
      721 ggagatcgcc gacgcggtgg acgcggatga gatcatctac ggagagcttt ccagggacgg
      781 gaccgtactc aggctaacga cgcgaaacct gctccgcgac cggaaaagcc tcgcaattac
      841 caagaaatcc atggtggttc tttcctttat tgatgaagag cttgatcact acgctgctga
      901 agcggctaaa aagcttattg agcccgcgta tgcgatacag aaacccgccg tgaaaacgta
      961 tgatggtgtt tcacttgctg ctgtaaagct tgaggaagtg aaaggcctgg acatagcggt
     1021 aatgcgtttt acatccgatg atgcgactat acaggccatg ttaacgtatt taaaggagct
     1081 ggttgaggaa tggggctcta tgcgaaactt tcgtaactgt tcggaagctt ctcaaagaca
     1141 atgcgttggc cgtgtaacaa caagggttta caaccccttg ttctggtgaa gacacaaagg
     1201 ccatcgttac ttatttccta cgatggaaaa gaaaggccta
//
LOCUS       DAOQXQ010000601         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_146421_length_1239_cov_2.127534, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000601 DAOQXQ010000000
VERSION     DAOQXQ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146421_length_1239_cov_2.127534"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1239
                     /locus_tag="PKH81_08960"
     CDS             <1..>1239
                     /locus_tag="PKH81_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98205.1"
                     /translation="NKTSVDWKNTMLASHEKREALEKIARKYEQNTGQYIRPINLIQV
                     ERTGKDQRASGMIHAEDVKEFLIKKCAISEDHVAIKSSEKDDIEGIDLLDPNCPIRYI
                     ITKQALQEGWDCPFAYILTILTNPSSQLSITQLVGRILRQPFARKTKIKDLDESYVFC
                     FKQKAVDLVKNIKVGLESEGLGDIAGRVTLDGGDDAGGLNLATREIQFRDKFKKFSGK
                     IYLPKFVIQEKDSWRDINFEVDILGAINWNDVDLKDLKTIELAKSKELEQELVYGFSQ
                     DKEKLIEEHDRKTRKGSLEINEVFLTRHIIDIIPNPWIAYEIGSKALQMFFKKYDKEI
                     VSSNFVYIIEEIKKALLKERDRMSEGVFRSLIKKGKLKFFLVVEKGGALPSRIKVRSN
                     KKLVRNDNSDIQMSLFDYVP"
BASE COUNT          409 a          231 c          271 g          328 t
ORIGIN      
        1 caacaagacg agcgttgatt ggaaaaatac catgcttgct tctcatgaaa agcgcgaggc
       61 gcttgaaaag atagcaagaa aatacgaaca gaataccggc caatatattc gtccgataaa
      121 tcttatacaa gtcgagcgca ccggtaagga ccagcgggcc agtgggatga tccatgcaga
      181 ggatgtgaag gaatttttaa tcaaaaaatg tgctatctcg gaagaccatg ttgccataaa
      241 aagcagtgaa aaggatgata tagaaggtat tgatttactc gatcccaact gcccaatacg
      301 atatatcata acaaaacagg ccctccagga gggctgggac tgcccctttg cgtatattct
      361 tactatattg accaatcctt cgtcgcaatt atctatcact cagctcgtgg gcaggatcct
      421 ccgtcaaccc tttgcgcgta aaacaaaaat taaagatctt gatgagtcat atgtcttttg
      481 ttttaaacag aaggcggttg atttagtaaa aaatattaaa gttggcctcg aaagtgaagg
      541 tcttggtgat atagccggca gggtaaccct tgatggtgga gatgatgccg gggggcttaa
      601 tcttgcgaca cgcgaaatac aatttcgtga taaatttaaa aagttttccg gtaaaatata
      661 tctcccgaaa ttcgttattc aggaaaaaga ttcctggcgt gatatcaatt ttgaagtaga
      721 tatactcggc gccatcaatt ggaatgatgt tgatttaaaa gacttaaaaa ctattgagct
      781 ggcaaaatcc aaagagctag agcaggagct ggtatacggg ttttctcagg ataaagaaaa
      841 gttaatcgag gagcatgacc gtaaaacaag aaaaggatcg ttggaaataa atgaggtatt
      901 cttgactcgt catatcattg atattatccc caatccctgg attgcctatg aaatcggctc
      961 aaaagccctg caaatgttct ttaaaaagta tgataaagaa atagtatcct ctaactttgt
     1021 atatatcata gaagaaataa aaaaggccct tctcaaagag cgagatcgca tgtctgaggg
     1081 agttttccgt tccttgatca agaagggaaa gcttaagttt ttccttgtag ttgaaaaagg
     1141 cggtgctctc ccctcccgca tcaaagtcag gagcaataag aaacttgttc gtaatgataa
     1201 ttccgacatt caaatgagcc tattcgatta tgtgcccga
//
LOCUS       DAOQXQ010000602         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_146911_length_1236_cov_3.712108, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000602 DAOQXQ010000000
VERSION     DAOQXQ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146911_length_1236_cov_3.712108"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKH81_08965"
     CDS             complement(<1..601)
                     /locus_tag="PKH81_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNQ98206.1"
                     /translation="MKKVIVASLFAACMGILSLPLLAQGEVIKLEAIASFYADDFHGK
                     PTSSGEIFDMHALTAAHKTLPFGTMLEVTNLANGRKVFVRVNDRGPFVEGRELDVSKG
                     AAQELGMLETGTARVSIRKIADNSGIATATNVAKETKVDGRWRIQLGSFTNEDNATRL
                     VMRLRSEGFNPAFEKSDGLTRVVLSGIAGAELDQTKGKLH"
     gene            788..1143
                     /gene="ssrA"
                     /locus_tag="PKH81_08970"
     tmRNA           788..1143
                     /gene="ssrA"
                     /locus_tag="PKH81_08970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          286 a          342 c          308 g          300 t
ORIGIN      
        1 ggtgtaattt accctttgtt tgatctagtt cagctccagc aataccggag agtacgacac
       61 gggtaagtcc atctgatttt tcaaacgcgg gattaaatcc ttcagagcga agtctcataa
      121 ccagtcgggt tgcgttatct tcattggtaa aggacccgag ctggattctc catcgtccgt
      181 ccacctttgt ctctttagct acgttagtag cggtggcaat gccgctatta tcggcaatct
      241 ttctgattga cacccgggcc gtacctgttt caagcatgcc aagctcttgg gccgcaccct
      301 ttgacacatc aagttctctt ccctcaacga agggaccacg atcattgacg cgaacaaaaa
      361 ccttccgccc atttgcaaga ttagttactt ccagcatcgt accgaaggga agcgtcttgt
      421 gcgccgctgt gagcgcatgc atatcaaaga tttccccgct ggaagtcggc ttcccgtgaa
      481 aatcatcagc ataaaatgaa gcaatcgcct caagtttgat aacttctccc tgtgccagaa
      541 gaggcaggct caatatgccc atacaagccg cgaacaggct tgctacaatc acctttttca
      601 tgtattcctc ccgaataaca gatcattact actatcggat tatctgggtg ctctcttaat
      661 tgttgtttca tgcgtggagg gatatatccc tttctttgct tgacactgga cgctaatact
      721 cgtatgtttg ctttgaaagt cgagggtttt ggcactgcca ggacccagcg attaagcttg
      781 cttaatcggg ggtgcaccgg tttcgactgt ggtggtcgac gcttgggctg caggtgaggt
      841 tgcctgtcct ctccaacggg taaaaactta taactgccga aacaaacgac agttacgctc
      901 tcgccgcgta agcagcagag cgcggaaacc ggatgatgct gctccgaagc atccgagctt
      961 tccgacgcca accggagctt gccaactgtt gggccagaac gacggaaggg acactaacct
     1021 ggatacggaa ccgacgcttg cggcactgcc accggatccc gacaactacc tcgtgtcgct
     1081 aaacctgtag acgcccctgc ctcgcacttc aggacggggg ttcgactccc cccatctcca
     1141 caaatgcaaa agccgcccga cagggcggtt tttgcatttg tgagggggag gagaacccga
     1201 ggcgcgtcgt cgcggccagg aaggccgcga agcgcc
//
LOCUS       DAOQXQ010000603         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_146993_length_1236_cov_2.363251, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000603 DAOQXQ010000000
VERSION     DAOQXQ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146993_length_1236_cov_2.363251"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>1236
                     /locus_tag="PKH81_08975"
     CDS             142..>1236
                     /locus_tag="PKH81_08975"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNQ98207.1"
                     /translation="MAKIQMKNAIAELDGDEMTRILWGVIKEKLLLPFIDLQVEYFDL
                     GLKARDDSDDRITYESAQAIKRLGVGVKCATITSNTARVEEYKLKGLTPSPNGIIRAE
                     LDGTVFRKPIIVKNIQPTVSCWTKPLVLGRHAYGDVYKNCEMYVDGPGKAELVFTGKD
                     GKEIRKTIMEMKGPGVLQGIHNLDKSIESFARACFYYGLSEKINVWFATKDTISKTYD
                     GRFKEIFQKIYDSEYKDQFEKAGIEYFYTLIDDVVARVMKNEGGFLWACKNYDGDVMS
                     DMVASASGSLAMMTSVLVSPDGKFEYEAAHGTVQRHYYKYLKGEKTSTNPVALIFAWT
                     GALAKRAELDKTPELADFAVKLEKATLDTIE"
BASE COUNT          360 a          254 c          314 g          308 t
ORIGIN      
        1 ttcgctgtgg aagatctcat gtctgtgttg tacgccggga agcaggcgat cgtcaagaac
       61 tgtctgccgg atttccgtat tattgcattg gaaactgatt ttggggtaca ctggatacca
      121 tcattataca aggaatactc tatggccaag atacagatga aaaacgcgat agccgaactg
      181 gatggagacg aaatgacccg tatcctgtgg ggcgttatca aggaaaaact cctgcttccc
      241 tttattgatt tacaggtgga gtattttgat ttgggtctta aagcacgaga cgacagtgac
      301 gaccggatta cctatgaatc ggcgcaagcc attaaacgct tgggtgtcgg ggtaaaatgc
      361 gcaacgataa cctctaacac cgcccgcgtt gaagaataca agcttaaggg acttacccca
      421 agccctaacg gcattatccg tgcagaactg gacggtaccg tttttagaaa accaattatc
      481 gttaaaaata ttcagccaac agtgtcttgc tggaccaagc ctctggtact tggccgacat
      541 gcatacggag atgtgtacaa gaactgcgag atgtacgtcg atggaccggg aaaagccgag
      601 cttgtgttta ccggcaagga cggaaaagaa attcgcaaaa cgattatgga aatgaagggc
      661 ccgggtgttt tacaaggaat tcataaccta gataaatcaa ttgagagttt tgcccgcgcc
      721 tgtttttact atggattatc cgaaaaaatt aatgtgtggt ttgcgacaaa ggacactatt
      781 tctaaaacct atgatgggcg ttttaaggaa atttttcaga agatttatga cagcgaatac
      841 aaagatcagt ttgaaaaagc aggcatcgag tatttttata ccctgatcga tgatgtcgtt
      901 gcccgcgtta tgaaaaacga gggcggattt ttgtgggcgt gcaaaaacta cgacggggat
      961 gtgatgagcg atatggtcgc ctctgcttca ggaagccttg ctatgatgac cagcgttctg
     1021 gtttctcctg acgggaagtt cgagtatgaa gctgcccacg gcaccgtaca aagacattac
     1081 tacaagtatc ttaagggaga gaaaacctcc acgaatccgg ttgcgctcat ctttgcctgg
     1141 accggagctt tagcaaaacg tgcggaactg gataagacgc cggaacttgc ggattttgcc
     1201 gtaaaactgg aaaaggcaac gcttgacacc atagaa
//
LOCUS       DAOQXQ010000604         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_147137_length_1235_cov_3.219492, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000604 DAOQXQ010000000
VERSION     DAOQXQ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147137_length_1235_cov_3.219492"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1173
                     /locus_tag="PKH81_08980"
     CDS             217..1173
                     /locus_tag="PKH81_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98208.1"
                     /translation="MNDKQHNISIFQEYYEDKPFVDRYLSTPDHAIDVIIPIIHANEL
                     WKANLMSFYREIPIHKLLIGDGGCIDDSMSIAEGFPRAQIFDHRSYKSLGYSIRKLIE
                     AVETEWFAYIHADAYLPAGWFDAMEKHQQEYDWFGCPMQHTVMIEYNVSYGERPWAGT
                     QMGRKKAFDSGLSRVDDDYVYRQEDYVFSDIVAKAGYREGKISDTFHYHQTMQKQTPW
                     SRHIKSVNFDLEISPEEEIRTCMTMAKGIVKYLDPPVLAEAAVLNIDRLVEMGVFSWR
                     DFMGWVKAENPDWVPVLKRQRLAMLPYKLKPVLRTIRNILGK"
BASE COUNT          342 a          264 c          312 g          317 t
ORIGIN      
        1 ttacccgccg gagcttggaa agttgcgtcg tttcggtgtc aaaatgttat atgccccgtt
       61 tttttgccag cccctccgct atatggacac ccatataacc ccatctattg ccaactggtt
      121 aaaacaggga agctggatga atgttattgc cgaaaaaagg tagttcccat caccgtaata
      181 gtttcattgc ttccatcaac aaaagaggtt acaggtatga acgataaaca gcacaatatt
      241 tccatatttc aggaatacta tgaagataaa cctttcgtag atcgctatct atccacaccg
      301 gatcatgcaa tagatgtcat tatccctata atacatgcaa acgagctttg gaaagctaat
      361 cttatgtcat tctaccggga gattcccatt cacaaactac taatcggaga tgggggctgt
      421 atcgatgatt ccatgagtat agccgaggga tttccccgtg cgcagatatt tgaccaccga
      481 agctataaat cgctgggtta cagcatccgg aaacttatag aagcagtgga gacggagtgg
      541 tttgcctata tacatgcgga tgcatatctg cccgcgggat ggtttgacgc gatggagaag
      601 caccagcagg aatacgactg gttcggatgc ccgatgcaac ataccgtaat gattgaatac
      661 aacgtgagtt atggagagcg tccctgggcg ggaacccaga tggggcgcaa aaaagcgttt
      721 gattcgggcc tgtcccgggt agatgacgat tatgtttaca ggcaggaaga ttacgtattt
      781 tcagatattg ttgccaaggc gggatacagg gaaggaaaga tttcggatac cttccattat
      841 caccagacca tgcagaaaca aacaccttgg tcgcgccata ttaaatcagt caatttcgat
      901 ttggagataa gtccggaaga ggagattcgt acctgtatga ccatggctaa ggggatagtc
      961 aaatatctcg atccgccggt tcttgcagag gcggcggtgc tgaatatcga tcgcttggtg
     1021 gaaatgggcg tgttcagttg gagggatttt atgggctggg tcaaggcaga aaacccggac
     1081 tgggtaccgg tgctgaaacg tcagcgtttg gccatgcttc cttacaagct caagccggta
     1141 ttgcgcacta ttcgtaatat ccttgggaaa tgagaaaaaa tgtcctgatt gcggtcagta
     1201 ttggaactca cttcatagag ttatcccgtg tcgca
//
LOCUS       DAOQXQ010000605         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_150313_length_1220_cov_2.620601, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000605 DAOQXQ010000000
VERSION     DAOQXQ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150313_length_1220_cov_2.620601"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1220)
                     /locus_tag="PKH81_08985"
     CDS             complement(<1..>1220)
                     /locus_tag="PKH81_08985"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNQ98209.1"
                     /translation="DIVHSHISAAYPVTAGGIFAAVAKMSFGNRIGVHLDSAAIEALS
                     IPEIFTRSNSGDTALTAPLYGSLIVETDGSGRDLLIKTIPSAVCIGHTTDEEAVVVGQ
                     ERIPLADAQHAWEAPLASVFPAVSSVTDQETLPAWACQLYQGQQASEGLRAPQVLRTA
                     KPRVIIPVFPGTNCEYDMERAFRLSGADTRILPLRNRTKEDLNASLDELRSAIRSSEI
                     LALSGGFSAGDEPDGSGKFIANILREHSIQEAILDLVHQRKGLVLGICNGFQALIKVG
                     LVPFGQITPPLESNPTLTYNTVGRHISRFSLTRIASNRSPWALGPGLEPGSVHCIPFS
                     HGEGRVIIQEELARRLFQNGQVFTQYVDQSGTPVMQEPDNPNGSLYAIEGMTDETGRI
                     LGKMGHNERPLDPGA"
BASE COUNT          272 a          308 c          369 g          271 t
ORIGIN      
        1 gcgcctggat caagaggacg ctcgttgtgt cccatctttc caagtatacg tccggtttcg
       61 tcagtcatac cttctattgc gtacagtgat ccgtttggat tgtcaggctc ctgcatgacc
      121 ggcgttccgc tctggtctac gtactgggta aagacttggc cgttttgaaa aagacggcgg
      181 gcaagctctt cctgaataat aaccctgcct tcgccgtgag agaagggtat gcaatgcaca
      241 gaacctggct caagtccggg gccaagagcc cagggggagc ggttggaagc aattcgtgta
      301 agcgaaaaac gggagatgtg acggcccacg gtgttgtatg ttagcgtggg gttactctcc
      361 aggggagggg taatctgccc aaagggaacc agcccaacct taataagggc ctggaaaccg
      421 ttgcagatgc caaggacaag ccccttacgt tgatgaacca gatcgagtat cgcctcttga
      481 attgaatgtt ctcgcaggat attggcaata aattttcccg atccgtcagg ctcgtctcct
      541 gcggaaaaac cgccggaaag ggcgagtatt tcgctggaac gtattgcgct tcgcagctcg
      601 tccagggatg cattcaagtc ctctttagtg cggttccgca aggggagaat acgggtgtcg
      661 gcgccagaga gacggaaggc cctttccatg tcgtattcgc agttagttcc ggggaacacc
      721 gggataatta cccggggctt ggcggttcgc aggacctgtg gcgcacgaag tccctcgctt
      781 gcctgttgtc cctggtacag ctggcaggcc caggcgggga gcgtctcttg gtcggttacc
      841 gaagaaaccg cggggaacac cgatgcaagc ggggcttccc aggcgtgctg tgcatctgca
      901 aggggaatgc gctcttgtcc cacaacaacg gcctcttcat cggtggtgtg gccaatacat
      961 acggcagagg ggatggtctt gataagcagg tccctgccgc ttccgtctgt ttccacaata
     1021 agggagccgt atagaggggc ggtaagcgcg gtatcgccag agttagagcg ggtaaaaatc
     1081 tccgggattg aaagggcttc tatagcagcc gaatcaagat gcacaccgat acggtttcca
     1141 aaggacatct tggctacggc agcaaagata ccgcctgcag taaccggata ggctgcgctg
     1201 atatggctgt gtactatgtc
//
LOCUS       DAOQXQ010000606         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_151014_length_1217_cov_2.068847, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000606 DAOQXQ010000000
VERSION     DAOQXQ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151014_length_1217_cov_2.068847"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKH81_08990"
     CDS             complement(<1..881)
                     /locus_tag="PKH81_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ98210.1"
                     /translation="MAQHPKSRAVSLLALSTAGSISCMLLALAVGTVWISPRSLFSAL
                     SSLNSPEPSLEKAIILSLRLPRVLLAFAAGAAFSVSGAALQAVLKNPLASPFTLGVSA
                     GASLGAGSVILFGLSSSLAAASGILSLASAPLAGFLMAMLTVGILLRFSRAVDPDLSG
                     TTVILAGMVISLFLNALLTLATALSRESVQRLVFWQMGSFALKGPYAVVVVWVTALAG
                     ISLLSLFHRELDILTFGDDEAVSLGVNSGRMRVILIAISAVMTGTAVSFVGVIGFVDL
                     AVPHAVRRIAGPAGGYAG"
     gene            complement(865..>1217)
                     /locus_tag="PKH81_08995"
     CDS             complement(865..>1217)
                     /locus_tag="PKH81_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98211.1"
                     /translation="ELSPAPALYSFGSGVYLDELLSIAGAHNCLADKTGWLAISAEEI
                     LARSPDVILTNTAFIDDPVHEILSRPGWTHIPAVQNHAVYKIDSNASSQPGPDVLVAL
                     REIYKAVYPNGTTP"
BASE COUNT          343 a          302 c          373 g          199 t
ORIGIN      
        1 cccgcgtaac cgccggcggg acctgcgatc cggcgcaccg catgaggaac ggcaagatcg
       61 acaaagccaa taactcccac aaaggaaacg gcggtacccg tcataaccgc cgaaatagct
      121 ataagaatta ccctcattcg ccctgagtta acgccaaggg agacagcttc atcgtctcca
      181 aaggtaagga tgtccagctc gcgatgaaag agactaagca ggcttatgcc cgcgagcgca
      241 gtaacccaca ccactacaac cgcgtaggga cctttaaggg caaaactgcc catttgccag
      301 aacacaaggc gttggacact ttcgcgggag agcgcggttg caagggtaag cagagcgttc
      361 aggaagagcg atataaccat tcccgcaaga ataaccgtag tgcccgaaag gtctggatct
      421 acggcgcggg aaaaacgtaa caaaatacca acagtaagca ttgccatcaa aaaacccgca
      481 aggggcgcgc tggcaaggga aaggataccg cttgcggcgg caagggagga gcttagtcca
      541 aaaagaatta ccgagccagc gcccagggat gcaccggcgg atacaccaag ggtaaaaggg
      601 gaggcaaggg ggtttttaag cacagcctgg agagctgccc cgctaacgga aaaggcagcc
      661 cctgcggcaa aggcaaggag tacccgggga agcctgagag aaagaataat ggccttttcc
      721 aaagagggtt cgggtgagtt aagcgaagac agcgcggaga aaagcgaccg aggggatatc
      781 cagaccgttc caaccgcgag ggcgaggagc atacaagaga tagagcccgc agtggacagg
      841 gcaaggaggg aaacagcgcg cgatttaggg tgttgtgcca ttggggtata ccgccttgta
      901 aatttcccgc agggcaacaa gaacatcggg accgggttgg gaacttgcgt tactgtctat
      961 tttgtacact gcatggtttt gtaccgcggg aatgtgggtc catccaggcc tgcttagtat
     1021 ctcgtgcact gggtcgtcaa taaaggcggt gttggtaagg ataacatcgg gggagcgggc
     1081 aagaatctct tccgcgctta tggcaagcca cccggttttg tcggcaaggc agttgtgcgc
     1141 gcccgcgata gaaaggagct catcaagata gaccccagag ccaaagctat agagggcggg
     1201 cgctggtgat agttcaa
//
LOCUS       DAOQXQ010000607         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_152529_length_1210_cov_1.984416, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000607 DAOQXQ010000000
VERSION     DAOQXQ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152529_length_1210_cov_1.984416"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKH81_09000"
     CDS             complement(<1..798)
                     /locus_tag="PKH81_09000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNQ98212.1"
                     /translation="MARNIKIFDTTLRDGEQSPGCSMNLTDKLEVAESLAALGVDVIE
                     AGFAIASPGDFQSVQAIARNIKGVTVASLSRALEKDIETAWSAIKEAESPRIHTFLAT
                     SPLHMEYKLKKTPEEVYEQATRMVKFARNLCGDIEFSLEDASRSDPAFMYRVIEGVIN
                     AGASTINIPDTVGYAVPEEFASLITNIRSNVPNIDKAIISVHCHNDLGLGVANSLAAA
                     KAGADQIECTVNGIGERAGNAALEEIVMNLYTRKEYYDMSCRVDTTQL"
     gene            957..>1210
                     /locus_tag="PKH81_09005"
     CDS             957..>1210
                     /locus_tag="PKH81_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98213.1"
                     /translation="MQFIERKTVLDQLQCARSRSSIVVISGLYGAGKSTLLAQIAHLL
                     REERPPIRILTIDGENRDEQGRTGAELVRDAKALGVGPSA"
BASE COUNT          316 a          293 c          280 g          321 t
ORIGIN      
        1 caactgcgta gtgtccacgc gacagctcat gtcatagtat tctttccggg tatacagatt
       61 catcacaatt tcttcaaggg cggcattacc cgcacgttcc ccaataccat taaccgtgca
      121 ttcaatttga tcagcgccgg ccttagccgc agccagagag tttgccacac caaggccaag
      181 gtcgttatga caatgaaccg agattatcgc cttgtctatg tttggtacat tggagcggat
      241 attggtgata agcgaggcaa attcctcggg aaccgcataa ccgaccgtat cgggtatgtt
      301 gattgttgag gcaccggcgt taatgactcc ttcgataacc ctatacataa aggccggatc
      361 ggagcgggaa gcgtcttcca gggagaattc aatatcccca cacagattgc gggcaaactt
      421 gaccatacgg gtcgcttgct catacacctc ttccggggtc tttttaagct tatattccat
      481 atgcaacggg cttgtggcca ggaaggtatg aatgcgggga ctttctgctt cttttatggc
      541 actccatgcc gtttcgatgt ctttttccag ggcgcgagaa agagaggcaa cggttacccc
      601 cttgatgttt ctggctatag cctgaacaga ttgaaaatcc ccagggctgg caatcgcgaa
      661 accagcttca ataacatcaa cacccagggc ggcaagactt tcggcaacct caagcttgtc
      721 agtaagattc atactgcatc ccggagactg ctctccgtcg cgtaaggtag tgtcaaaaat
      781 cttaatgttg cgagccatac aaaaacctcc tcatcgcctt tgtataacga tgtatctgaa
      841 atgtaccgga ttgaattacc atggacaagc agagaaaact catttcagat aatttattgt
      901 attttatata gtacaaatat tgtattaata catatttgta gattattatt gacaatatgc
      961 agtttattga acgaaaaaca gttcttgatc agctacaatg tgctcgctcc agatcatcca
     1021 ttgtggtcat ctctggtctc tatggagcgg gtaaatccac gttgctcgcc caaattgccc
     1081 acttgcttag agaagagcgg cctccgatca gaattctgac cattgatggc gagaatcggg
     1141 acgaacaagg tcgaaccggt gcagagcttg tccgtgacgc aaaagccctt ggcgttgggc
     1201 cgtcggcctt
//
LOCUS       DAOQXQ010000608         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_152656_length_1209_cov_2.898614, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000608 DAOQXQ010000000
VERSION     DAOQXQ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152656_length_1209_cov_2.898614"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..826)
                     /locus_tag="PKH81_09010"
     CDS             complement(8..826)
                     /locus_tag="PKH81_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98214.1"
                     /translation="MINLKQSLSTGITILFLSLLAVSCAQEADDEARLNPEETFTQVD
                     APLSYTVAVLNVTGPWGYQKSVNKERLYPLLVSGCWGEGESNYQKIEQRYPAFVIDYQ
                     KNGVSDGEKLADWIDLAIAEGYRIDPNRIYLTGFSMGGSGSFPLARGMHNKGKFFAAI
                     IRVAGQSESDLGEEIAKETAVWYHIGLTDTEKRVQVARQALETFRVYACNKKAKETTE
                     TDDITGHERTTITLTRSGTPYFKYSEYTDMGHTPSPCYKDKELFPWMFSMSLDN"
     gene            966..>1209
                     /locus_tag="PKH81_09015"
     CDS             966..>1209
                     /locus_tag="PKH81_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNQ98215.1"
                     /translation="MLDNQLKKAINEQINKEFYSSYLYLAMAAHFDANALPGFAHWMK
                     VQAAEEWEHGMKFYEYLYEVGAEVELAAIPCPPLKFG"
BASE COUNT          292 a          265 c          303 g          349 t
ORIGIN      
        1 taacccatta gttatcaaga ctcatggaga acatccaggg aaagagttct ttatccttat
       61 aacagggcga aggggtgtgc cccatatctg tgtattcgct gtatttaaaa tagggcgtac
      121 ccgatcgggt aagggttatg gtggtgcgct cgtgaccggt aatatcgtcg gtctctgttg
      181 tttcttttgc ttttttatta cacgcataca cgcggaatgt ttcaagagct tggcgtgcaa
      241 cctggactcg cttttcggta tcggtaagcc cgatgtgata ccaaacggcc gtttctttgg
      301 ctatctcttc gccaagatcc gattcgcttt gacctgcaac cctgatgatt gcggcaaaaa
      361 acttgccctt gttgtgcatg ccgcgggcaa gggggaaact tcccgaacct cccatggaaa
      421 atcctgtcag gtatatccgg tttggatcga tgcggtagcc ttcggcaatt gccaggtcta
      481 tccagtccgc gagcttttct ccgtcggata ccccgttttt ttgatagtca ataacaaagg
      541 ctggataacg ctgttcaatt ttctggtagt tactctctcc ctctccccag catcccgata
      601 ccaaaagcgg gtagagccgt tctttattta ccgatttttg gtatccccag ggaccagtta
      661 cattaaggac ggctacagta tatgaaaggg gcgcgtcgac ttgggtaaag gtttcttcag
      721 gattaaggcg agcttcatcg tcagcctcct gtgcgcagga aaccgctagt aaagaaagga
      781 aaaggatggt tataccggtg gaaagtgatt gttttaggtt tatcatgggt tctccttacg
      841 caagcgcttc ctagcatact atcacgtttt atgccgaaat ctcagaatta ccaacaatcc
      901 tgctaacagt aactaaaagt tgctttgaac cggtatcgtg attatacttg ttataggagg
      961 ataacatgtt ggataatcaa ttgaaaaagg cgataaacga gcagattaac aaggaatttt
     1021 attcatcgta tttgtacttg gctatggcgg ctcattttga cgccaatgcc ttaccggggt
     1081 ttgctcactg gatgaaggtt caggctgccg aggaatggga acatggaatg aagttctatg
     1141 agtacctgta tgaggtcggg gctgaggttg agcttgcggc gataccctgt cctccactga
     1201 agttcgggt
//
LOCUS       DAOQXQ010000609         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_152919_length_1208_cov_2.335646, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000609 DAOQXQ010000000
VERSION     DAOQXQ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152919_length_1208_cov_2.335646"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1208
                     /locus_tag="PKH81_09020"
     CDS             <1..>1208
                     /locus_tag="PKH81_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RarA"
                     /protein_id="HNQ98216.1"
                     /translation="EVNKALVSRSRVFQLKALTSADLARVAQFALTDKERGYGRWNVS
                     FAPDALEHLVQTASGDARSLLNALELAIESSVPSWPPADGTAIAIDRDTAEESIQQRI
                     VLYDRDGDYHYDVISAFIKSLRGRDPDAALYWMARMLVAGEDPHFIFRRMLISAAEDT
                     GLADPMAIVVVESCAAAFDRIGLPEGRFHLTQAALYLATAPKSNSTLAFFDAIASVEG
                     EDAEVPNHLKDGNRDKEGFGHGEGYMYPHAYRDHWAAQQYLPSSLVGRVFYTPSRQGH
                     EGAIRQEVLTRRELQIAAILEEKDESRAEGEMLTFSPGNSKRNQWVKRLDSGTSESLL
                     RIREALFENCALARHHRILVWGADDGLLIWEASRRAPEGFVAGLCRREQALGELEQYA
                     RTLDELDRPQL"
BASE COUNT          297 a          319 c          330 g          262 t
ORIGIN      
        1 gaagtaaaca aggcgctggt aagcagaagt cgggtatttc aactcaaggc tcttacctcc
       61 gccgatcttg cccgggttgc acagttcgcc cttacagaca aggagcgcgg ttatggcaga
      121 tggaatgtca gctttgcccc ggatgccctt gaacacctgg tacagacagc atcgggcgac
      181 gcccgaagcc tgttaaatgc ccttgaactt gccattgaat cttcggtacc ctcttggcca
      241 ccggcagatg gaacggccat agcgatagac cgggacacag ccgaagaaag catccagcaa
      301 agaatcgttt tatacgaccg cgacggtgat tatcactatg acgttatcag cgcgtttatc
      361 aaatccctgc gagggagaga cccggacgcg gccctgtact ggatggcgag gatgcttgtt
      421 gcgggagaag atcctcactt tatttttaga cgaatgctta tatccgcagc cgaagacacc
      481 ggccttgccg atcccatggc cattgtggta gttgaaagct gcgccgctgc ctttgaccgc
      541 ataggcttgc ccgaaggacg ctttcatcta acgcaggcag ccctgtacct ggcgacagca
      601 ccaaaatcga acagcacgct cgcttttttt gacgctatcg cctcggtgga aggggaagac
      661 gctgaggttc cgaaccatct taaagatgga aatcgtgata aagagggttt tggacacgga
      721 gaaggatata tgtatcctca tgcttaccgc gaccattggg ccgcccagca atacctgccc
      781 tcatcactgg taggacgcgt tttttatacc ccatccaggc aaggccatga gggtgcaatt
      841 agacaggaag tcctgacacg acgggaactg cagatagctg ccattttgga agaaaaagac
      901 gaatcccgcg ccgaaggcga aatgttaacc tttagccccg gcaattcaaa acgtaaccaa
      961 tgggtcaagc ggcttgacag tggaacatct gaatccttac tgcgaatcag ggaggcactg
     1021 tttgaaaact gtgccctggc gcgccaccat cgcatccttg tgtggggtgc cgatgacggc
     1081 ttgcttattt gggaagcttc ccgacgagcc ccggaaggtt ttgtggcggg cttatgccga
     1141 agagagcagg ctcttggaga acttgaacag tatgcgcgca cccttgatga attagaccgt
     1201 ccccagct
//
LOCUS       DAOQXQ010000610         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_153132_length_1207_cov_2.201389, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000610 DAOQXQ010000000
VERSION     DAOQXQ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153132_length_1207_cov_2.201389"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1104)
                     /locus_tag="PKH81_09025"
     CDS             complement(172..1104)
                     /locus_tag="PKH81_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ98217.1"
                     /translation="MESKKIKKSISRFFAWVGLNTCVLSGKILPQPLLYGFAGMVARM
                     GYLCAVKQRKVAIESLKTAFGTEKSDEERKKIARQCFAYMAKSGVEIMFLVDKPELMR
                     RKVSFVHKERLDKALEQGKGVVLVSAHFGNFPLLLGRLSLEGYRTGGIMRPMRDARAE
                     KIFLQKRERYGIKTIYSQPRKECVEETVRSLRNNEIIFIPLDQNFGTGGIFVDFFGTK
                     AATATGPAILAQRTQAALLPCFIVRQPDDTHKIIFEEPLMIPGGGTTRGPAQVVEVIQ
                     SLTRIIESYIRQYPAEWGWIHRRWKSKPSVKGGE"
BASE COUNT          295 a          357 c          280 g          275 t
ORIGIN      
        1 aacaggaata aaccgataca ccccctgacc acaacgagca ggtcgcacca ccattgataa
       61 accgccagtg cgcccaaggc caaaaagaca agacccagga gtaccttgaa gacggtccag
      121 aacacctttg cgccgtctgc ctttgcctcc tgctttaact cttgagccat ctcactcacc
      181 tcctttaacg cttggcttac tcttccatct ccggtgtatc cagccccatt cagccggata
      241 ctgcctaata taagattcaa tgatccgcgt gaggctttgg atgacctcga cgacctgggc
      301 tgggccacgg gtagtacccc cgccaggtat catcagcggt tcctcaaaaa tgatcttgtg
      361 ggtatcgtcg ggctgccgca cgataaaaca aggcagcagc gccgcctgag tcctttgagc
      421 cagtattgcc gggccagtgg cagtggcggc cttggtaccg aagaaatcaa cgaaaatccc
      481 cccggttccg aaattctggt ccaggggaat aaaaataatc tcgttattgc gtaaagacct
      541 gactgtctct tcgacgcatt ccttgcgcgg ctggctgtat atcgttttga tgccgtagcg
      601 ttcgcgtttc tgaaggaata tcttttccgc gcgcgcatcg cgcatcggcc gcatgatccc
      661 gccggtccgg tatccctcca ggctcaggcg ccccagcaag agggggaagt tcccgaagtg
      721 cgcgcttacc agaacaactc ccttaccctg ctccagggcc ttatccaggc gctccttatg
      781 cacaaagctc actttcctgc gcatgagctc aggcttatcc actaaaaaca ttatctcaac
      841 cccggacttt gccatatacg caaagcactg cctggctatt ttcttacgtt cctcatcgga
      901 cttttccgtg ccaaaagcgg ttttaagact ctcgatagct accttgcgct gtttgaccgc
      961 gcacagatac cccatgcggg caaccatccc ggcaaaccca tagagcaaag gttggggcag
     1021 tatcttgccg gacaaaacac aggtattcag gccgacccag gcaaagaaac gcgaaatgct
     1081 tttcttaatc ttcttggatt ccatattttt atagagcagg attataacaa aaggggcaaa
     1141 aaatgcaagg taaaagttac cgcttatagc cgatagtgcg caggaattcg ctgcgccagg
     1201 ccaagtc
//
LOCUS       DAOQXQ010000611         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_153283_length_1206_cov_2.894005, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000611 DAOQXQ010000000
VERSION     DAOQXQ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153283_length_1206_cov_2.894005"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1206
                     /locus_tag="PKH81_09030"
     CDS             <1..>1206
                     /locus_tag="PKH81_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNQ98218.1"
                     /translation="LFFLLMQMALTSFDLLQTGREYLKAGMENQTITAEKDTDEGLIG
                     KHVKSYHSARFLQNQQIRKSFSVLVLRSFFCFLLAMRLLSLLKQELRNPLVTVSSKLD
                     ILTSHGGKETGLIDIVTNDEFTPIFRAINTLIEQQGEQVQSSLDRLDSIIEHAADPVI
                     SFNDEGCILIFNPAAQRYFGKSRDEAERMILKDLFTRDEIDYCGCGEDNRAFVEYLIS
                     EQNKLRRFSGKGEGRVIHFEANISKTRSVEGPVYTAILRDISAQIEIEENLTRAKIAA
                     ENANRLKSEFLANMSHELRTPLNAVLGFTQLLMSDDTIGESQKEKIKIISRSGEHLLG
                     LINDILDISKIEAGKAELNTSVCAIHRFLEEIHEMFTLRCEKKGLNLDIEILDTLPEY
                     IEVDTGKLR"
BASE COUNT          336 a          253 c          295 g          322 t
ORIGIN      
        1 cttgtttttt cttcttatgc aaatggcgct aacgtctttt gatttattac aaacagggcg
       61 ggaatatctt aaagctggaa tggaaaatca aaccatcaca gcggagaaag atactgacga
      121 aggtttgatt ggcaagcatg taaaaagcta tcactccgcg cggtttttgc aaaaccagca
      181 aattcgtaaa agttttagcg tgttggtgct tcgctctttt ttttgtttcc ttttggcaat
      241 gcgtctcttg tcgcttctta aacaagagct ccgtaatcct ttagtaaccg tatcttccaa
      301 gctggacatt cttacctctc atggaggaaa ggaaacaggc cttattgata ttgttaccaa
      361 tgatgagttt actccgatct tccgggcaat aaacaccctg atagaacagc aaggggaaca
      421 ggtccaatcg agccttgatc gccttgattc gattatagag catgctgccg atccagtaat
      481 ctcgtttaat gatgaaggct gcatcctgat ttttaatccg gcggctcagc gttattttgg
      541 taaaagcagg gacgaggctg agcggatgat ccttaaagat ttatttaccc gcgacgagat
      601 tgattactgt ggttgtggcg aggataaccg cgcctttgtg gaatatctta tcagcgagca
      661 aaataaactc aggcgatttt ccgggaaggg cgaggggcgg gtaattcatt ttgaggcgaa
      721 tataagtaag acgcgcagtg tggaaggccc tgtgtataca gcaattttac gcgacattag
      781 cgctcagata gaaatagaag agaacctgac ccgggccaag atcgccgcag aaaacgcgaa
      841 ccgcttaaaa agcgagtttc ttgccaacat gagtcatgag ctacggactc cccttaacgc
      901 ggtgcttggg tttactcaac tccttatgag cgacgacacg attggcgaga gtcaaaagga
      961 aaagatcaag attatctccc gaagcgggga gcatctgttg ggccttatta acgacatcct
     1021 tgatatctcc aagattgagg cgggcaaggc ggaactgaat acctcggtgt gcgccattca
     1081 tcgctttttg gaagagattc acgagatgtt tacccttcga tgtgaaaaaa aaggattgaa
     1141 cctggatata gaaattcttg acacccttcc tgagtacatc gaagtggata ccggtaagct
     1201 caggca
//
LOCUS       DAOQXQ010000612         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_153311_length_1206_cov_2.442224, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000612 DAOQXQ010000000
VERSION     DAOQXQ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153311_length_1206_cov_2.442224"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKH81_09035"
     CDS             <1..624
                     /locus_tag="PKH81_09035"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNQ98219.1"
                     /translation="IFEFIYFARPDSVMDGRSLYHARVDMGRMLAKEHPVKADYVIPV
                     PDSGTPAAIGYASKSKIPFSDVLVKNRYVGRTFIQPTQDIRELGVKLKLNPIKQVING
                     KDIVLVDDSIVRGTTSRQIVRMLKDSGARKVHMRVASPPIIGTCRFGIDTASKKELIA
                     SYMSVEEIRRYLGVDSLGYISLEGLEKAISLKNDPLCMGCLNLKYPS"
BASE COUNT          339 a          282 c          268 g          317 t
ORIGIN      
        1 atattcgagt tcatctattt tgccaggccc gacagcgtta tggacggcag aagcctttat
       61 cacgcccgcg ttgatatggg gcgcatgctt gccaaggaac atcccgtaaa agccgattat
      121 gtgatccctg tccctgattc cggcacgccc gccgccatcg gatatgcttc aaagtccaag
      181 atcccgttca gcgatgtcct tgtcaagaac cgctacgtgg ggcgcacttt catccagccc
      241 acacaggata tcagggagct tggagtaaag ctaaaactca atcctatcaa acaggtgata
      301 aacggcaagg atatcgtgct ggtggacgat tcgatagtac gaggaactac aagccgccag
      361 atagtcagga tgctcaaaga ctccggcgcc cgcaaggtgc acatgagagt ggccagccct
      421 ccgataatcg gcacgtgcag gttcggcata gataccgcct caaagaaaga gctgatcgcc
      481 tcgtatatgt ccgtggaaga gatccgccgg tatcttggag tggattccct aggatacata
      541 agccttgaag ggctggaaaa ggccatttcg ttgaaaaacg atcctctttg tatgggatgt
      601 ttgaacttaa aatacccatc ctaagcagac tacagaaatt cacattctcg ccaagcgatg
      661 gaaaccgatt tttgtaagac accagagaat caagcacaac acggttattt cgctcgtttc
      721 gaaacgattg ggtaatgcac gaaacgaata taccacctag gtcaacattg ttaggttgaa
      781 tttcaagggc ccttgcaaag aaggcttttg cccgaataaa attaccttga ttgtaaaaag
      841 cccgtccact ttcataatag tagacaaaag aaatttcatc taatatctcc tggtcggtgg
      901 tattttgaat tacggcttca aaacattttt tatagagttg tttatcattg ttcttaaaca
      961 aaacagtttg agtaaggttg taaaactcac cctttatcat ttctgcagtt attccctttt
     1021 cctttagcct ggatattcgc ccgataagcc ctgctttttt tagcgggtca agattggagg
     1081 tctccaatct ggccgcagaa aaatgcaaca atagatactc tgaacgaaca ttggggtaaa
     1141 accaataacc cttttttatt tgctcgtagg cccccgcgat atcgttatgg tcttgtctaa
     1201 ataagg
//
LOCUS       DAOQXQ010000613         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_153759_length_1204_cov_2.416014, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000613 DAOQXQ010000000
VERSION     DAOQXQ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153759_length_1204_cov_2.416014"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKH81_09040"
     CDS             complement(<1..914)
                     /locus_tag="PKH81_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ98220.1"
                     /translation="MIDRLGEIAALGTSVCWTVTALAFEYAGKRIGTLALNILRLAVA
                     LLIFAIVGLIVRGNPIPLDAPPELWFWLSLSGLVGFVFGDIFLFQSYIDIGARKALVV
                     FVSSPLITALLGFGLLGEKISPVGIAGMCIVIAAITWVVFGSREDTSESLAVAASRKS
                     RQTRGVLFALLGALGQSGGLVLSKMAAPSYDAIAATQIRVIAGLVGFTIVALFLRRSR
                     ECLAAIQNIPALKILSIGAFFGPFLGVSLGMFAIQRTSSGIAATLMGLTPILILVPSV
                     LIRKERITIREVLGSALAVFGSALIFLH"
BASE COUNT          365 a          305 c          277 g          257 t
ORIGIN      
        1 caatgcaaaa aaatcagggc agagccaaag acagccagag cagaaccaag aacttcccga
       61 attgtgattc gttccttgcg aattaacacc gaaggtacaa gaatcaggat tggtgttaag
      121 cccattagcg ttgccgcaat tccggacgac gttctttgta tggcaaacat accaaggctg
      181 actcccaaga aggggccaaa aaaagcgcct atggataata tctttaaggc agggatgttt
      241 tgtatggcag ctaaacattc ccgggatcgc cttaagaaca gggctacaat ggtaaatcct
      301 acaagccccg cgataactcg aatctgtgtc gcagcaatag catcgtatga gggtgcggcc
      361 atcttggaaa ggacaagccc gcccgactgc ccaagggcac ccaacaaggc aaagagcacg
      421 cccctagttt gtcttgattt tctggaagca gctaccgcaa ggctctcgct cgtatcttcc
      481 ctggatccaa aaacaaccca ggtgattgcc gcgataacga tacacattcc cgcaattccg
      541 actggagaga ttttttcccc aagaagccca aaaccgagca aggcagtgat gagcggggaa
      601 gatacaaaca cgaccagggc ttttctggcc ccgatgtcta tataggattg aaataaaaag
      661 atatcaccaa aaacaaaacc aacaagaccg gaaagagaca gccaaaacca gagctcgggc
      721 ggagcatcca ggggaatggg attgccgcgc acaatcaggc caacgatggc aaagatcaac
      781 aaagcaacgg caagcctcaa tatgttcaag gctaaggttc caattcgctt gcctgcatat
      841 tcaaaggcga gcgcagtaac cgtccagcat accgaagttc ccaaagccgc aatctctcca
      901 aggcgatcta tcatgatact tccgtccaac gcgattgatg gcgtcagcgt atcacagaac
      961 cccaggcgat ttctactata cgctcttggc taaaaaaact tggcagtaat taccacatta
     1021 gtagcgggca tgataaaagt ggaagtacca ccacttgtgg taattaccgc ggacgttact
     1081 gttattcctg gaatgtttat acctacctta cgatttggtc caagagtcgc cgtcagggta
     1141 atggcctctc taaactgcgc ccttttgtca acacattttt tatcgcagtt taaggtgtat
     1201 tatg
//
LOCUS       DAOQXQ010000614         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_154166_length_1202_cov_2.405405, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000614 DAOQXQ010000000
VERSION     DAOQXQ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154166_length_1202_cov_2.405405"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKH81_09045"
     CDS             complement(<1..597)
                     /locus_tag="PKH81_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNQ98221.1"
                     /translation="MTNLTNLLHSFRECAQTEREKGTYFERLIQDYLTVEPAYADLYE
                     KVWMYGDWAREQGIDARDTGIDLVALTFNSEYHAIQCKLYAEDYTLQKSDIDSFFTAS
                     GKKPFTHRIIVSTTNKWSVHAEAALADQQPPVTKIDLRALEESVIDWARYKSGKTVVS
                     KPQKKLRKHQEEAIKEVVKGLSVADRGKLLMACGTGKTF"
     gene            782..>1202
                     /locus_tag="PKH81_09050"
     CDS             782..>1202
                     /locus_tag="PKH81_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98222.1"
                     /translation="MTLLVPGRHHILTDFQSSYLFRFCNAGMDGELDMEGRPLSGRID
                     RVVFAVTSADHSLTRRNPLPFFARAMALHELGSNLGINLLVYGIPEAGERPDFAEYTL
                     KTIFHESEGLVSLTPDDCIVACATTVAELYRKAGFRVW"
BASE COUNT          286 a          303 c          273 g          340 t
ORIGIN      
        1 gaaggttttc cccgtaccgc aagccataag gagctttccg cggtcggcaa ccgacaaacc
       61 ttttacaacc tctttgattg cctcctcttg atgcttcctt aacttctttt gaggcttact
      121 aacaactgtc ttaccgcttt tataccgtgc ccaatctatg acagattctt ccagggcacg
      181 aaggtcaatc ttggttaccg gtggctgttg atccgcaagg gccgcttccg cgtgtacgct
      241 ccatttattg gtagtgctta cgataattcg atgggtaaaa ggtttcttcc ccgaggcggt
      301 aaagaagctg tcaatatcgg atttttgcag ggtatagtcc tcggcataga gtttgcattg
      361 gatggcgtgg tattcactgt tgaaggtaag agcgacgagg tctatacccg tatcccgtgc
      421 gtcgatcccc tgctcccggg cccaatcgcc atacatccat accttctcat aaagatccgc
      481 ataggcaggt tcaacagtaa ggtaatcctg aatcaggcgt tcaaaatacg tgcccttttc
      541 acgctcggtt tgggcacact cgcgaaaact atgtaaaaga ttggtcaggt ttgtcataag
      601 gatagctcat ctccattttt ttccaggaca acttcataat atcacttaaa agaggtaaat
      661 gggctattga ttgcagaaat ccacttcttt actgcaatac cttttatttt catctccata
      721 taggacatca acttcatttc cgatattatc gcgtcatgat atcatagcta tggaggatta
      781 catgacgctt cttgttcctg gccgccatca tatactgacg gactttcaat cttcgtactt
      841 gttcagattt tgcaacgccg gaatggacgg tgaacttgat atggaaggcc gaccgctctc
      901 gggccggatt gaccgggtcg tctttgccgt taccagtgcc gatcattccc tgacccgcag
      961 gaatcccctg ccattttttg cccgggcaat ggcgctccat gaactgggat caaaccttgg
     1021 catcaacctt cttgtatacg gtattcccga agcaggcgaa cggcctgatt ttgccgaata
     1081 cacgctcaag acaatatttc acgaaagcga aggattagta tcccttacgc cagatgattg
     1141 catagtggcc tgcgccacca cagtggcaga gctgtatcga aaggcgggct ttagagtatg
     1201 gg
//
LOCUS       DAOQXQ010000615         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_154584_length_1200_cov_3.635808, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000615 DAOQXQ010000000
VERSION     DAOQXQ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154584_length_1200_cov_3.635808"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="dctP"
                     /locus_tag="PKH81_09055"
     CDS             complement(<1..341)
                     /gene="dctP"
                     /locus_tag="PKH81_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNQ98223.1"
                     /translation="MFQKKNLLIALIITLVAFSVFPQQKIKLKIASVAPARSPWDIEQ
                     RALAQEWSKITGGRVSVAFYDSFSLGGEKAVIQKFRSVRPGQKPMLDGAIFSTIGLNE
                     LAPQANIYTLS"
     gene            complement(351..>1200)
                     /locus_tag="PKH81_09060"
     CDS             complement(351..>1200)
                     /locus_tag="PKH81_09060"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNQ98224.1"
                     /translation="PPSDKPIEGANPMAALTGENDPQLVKEFFPTALKLYEIMHLQNP
                     QHEELAVMTGQLYVMYANAFIQGPAEQLPPEDFTMQEEQYRRAGVFYIRGKDFALKAL
                     QKRYKGFTDAVFGLDRDAAQTMLSSCSKKDVPALFWAASGLMGAFSLSPLETSYLELL
                     PGSLAMIERASILDPTYNNGAIWEILAAFYAAAPESLGGGKDKAQQAYTKALEITGGK
                     SPGTHILYARSFCIPAQDGQGFDEHIDKALAINPEDDPDNRLVITIAQRQARWLKEHK
                     ADFILE"
BASE COUNT          294 a          268 c          332 g          306 t
ORIGIN      
        1 atcgagaggg tatagatatt tgcctgagga gcaagctcgt tcaggccgat ggtggaaaag
       61 attgccccat cgagcatcgg tttttgaccg ggacgaacgg aacggaattt ttgaattacc
      121 gctttttcgc caccaaggga aaaagagtcg taaaaggcaa cgctgacgcg tccgccggtg
      181 attttggacc attcttgagc aagggcacgc tgttcaatat cccaaggaga tcttgcggga
      241 gcgacagagg cgatcttgag cttgattttt tgctggggaa aaaccgaaaa ggcaacaagg
      301 gtaatgatga gtgcgatcaa taaattcttt ttctgaaaca tgtgaatctc ctattcaaga
      361 atgaagtccg ctttatgctc cttgagccag cgggcttgtc gttgggcaat ggtaatgaca
      421 aggcggttgt ccgggtcatc ttccgggtta atagctaagg ctttgtcgat atgttcgtca
      481 aatccctgtc catcctgtgc cggtatacaa aagctgcggg catagaggat gtgtgtaccg
      541 gggcttttcc cgccagtaat ctcgagtgcc ttggtgtatg cctgttgagc cttgtctttg
      601 cctcctccaa gcgattctgg cgcggcggcg taaaacgcgg cgagtatctc ccaaatagca
      661 ccattgttgt aggtgggatc aaggatactc gctcgttcta tcatggcaag ggatcccggc
      721 aagagttcaa gataacttgt ttcaagcgga cttaacgaaa aggctcccat aaggccggag
      781 gcagcccaaa agagggcagg gacatccttt tttgagcagg aagagagcat ggtctgtgcc
      841 gcatcccgat caagaccaaa aaccgcatca gtaaaacctt tgtatctttt ttgtaaggcc
      901 ttaagggcaa aatccttacc tctgatatag aacactccag ccctgcggta ttgttcttcc
      961 tgcatggtaa agtcttccgg agggagctgt tcagcaggcc cctggataaa ggcgttggca
     1021 tacataacat acagttggcc agtcataact gccagttctt cgtgttgggg attttgcaga
     1081 tgcataatct cgtagagctt aagggcagtg ggaaagaact ccttaacaag ctgggggtca
     1141 ttctctccgg taagggcggc catcgggtta gccccttcta tgggtttgtc tgagggaggg
//
LOCUS       DAOQXQ010000616         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_155312_length_1197_cov_2.513135, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000616 DAOQXQ010000000
VERSION     DAOQXQ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155312_length_1197_cov_2.513135"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1197)
                     /locus_tag="PKH81_09065"
     CDS             complement(<1..>1197)
                     /locus_tag="PKH81_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ98225.1"
                     /translation="LAFRFTVDKIYQRGSALPVLYFISFFYAAIAIASQLTERRRVSQ
                     ETRKAIFIFVPVTMVPVLVQFAFPAILVQGLGLAFSEMIILLTVQDFGRYFNSSTGLY
                     NRDGLIARLNAVNWKRIPFTVYLVCLDSVEFLQNALGSEDFTALEKEVSKRLKQEPLH
                     MQVVSRISPGNFALVSTKRESMQEQENILEGLFTGCWRIEHHELSLNAHLCAIRFPED
                     CRDIERIFLAQNHLSRFSQHYPENHILRLEDFSLAQTDHQLTAAQALKEAVRDNGFEV
                     FYQPIVESSTGKVIAAEALVRLSEKRHAKISPAQFIPLAELDGSIYTIGEFVLEKSAC
                     FLAQLRQGGHDLAYFEVNLSALQCLQTDIVETILSRTRKYTLESSDFCLEITETATRL
                     SPALMN"
BASE COUNT          312 a          261 c          312 g          312 t
ORIGIN      
        1 cgttcatcaa ggcaggagag agtcgcgtgg ccgtttcggt tatctccaga caaaaatctg
       61 aactttccaa tgtatacttg cgagttcggg acagtatggt ttctacaata tcagtctgga
      121 gacattgaag ggcggagagg tttacttcaa agtatgcaag gtcgtgtcct ccctggcgga
      181 gctgggcaag gaagcatgcg gatttttcca gcacaaactc accgatggta tagatgctgc
      241 catccagttc ggccaatggt ataaattgag ctggagagat ttttgcgtgg cgtttttcgc
      301 tcagccgaac cagggcttct gccgctataa ccttgccggt tgatgattca actattggtt
      361 ggtaaaacac ttcaaatcca ttatcccgaa ctgcttcttt aagagcttgg gctgcggtaa
      421 gttggtgatc tgtttgtgca agagaaaaat cctccagccg taagatgtga ttttccggat
      481 aatgctgaga aaaacgtgaa agatgatttt gcgccaggaa gatgcgttct atatcgcggc
      541 aatcttcggg aaagcgaatg gcacaaagat gggcgtttaa cgaaagttca tggtgttcta
      601 ttctccagca cccggtaaac aggccttcaa gtatattctc ctgttcttgc atgctttcgc
      661 gcttggttga caccagggca aaattcccgg gggatatgcg ggagactacc tgcatgtgca
      721 ggggttcttg cttcaggcgt ttacttactt ctttttcaag ggcggtaaag tcttcgcttc
      781 ccagtgcatt ttgcaagaac tcaacggaat caaggcagac tagatatacg gtaaaaggaa
      841 ttcgtttcca gttaaccgcg ttaaggcgcg ctatcagtcc gtctctatta tataaaccgg
      901 ttgagctatt aaagtagcgg ccaaagtcct ggactgtcag gaggattatc atttccgaga
      961 aggcgagacc aagcccttgt accaatatgg ccgggaaggc aaactggacc agaacgggaa
     1021 ccatggtaac cggcacaaag ataaaaatcg cttttcttgt ttcctggctt acccgtctgc
     1081 gctcggttaa ttggctcgca atggcgatgg ccgcgtaaaa aaaggaaatg aaatacagga
     1141 cgggcagggc gcttccacgt tgatatatct tgtcgacggt aaaacgaaag gccagac
//
LOCUS       DAOQXQ010000617         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_156837_length_1190_cov_2.912775, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000617 DAOQXQ010000000
VERSION     DAOQXQ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156837_length_1190_cov_2.912775"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1023
                     /locus_tag="PKH81_09070"
     CDS             88..1023
                     /locus_tag="PKH81_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98226.1"
                     /translation="MAVMMSAVTESKKNGTKKGVAKNHERALFYALLLPAFILVTLFI
                     IYPVVNTIFYSFYDWNGFGPLKNFVGLKNLRTLLSDPLFWNALRNNGIVILFSIGIQL
                     PIAFLLAILVTRSTNRLTIFFRTVFFLPYILSEIITGVIWRFVYHPQFGLLQQVFKFF
                     SPEYSYTGLLADPKTVFIAILFVIFWKYFGFHMILYVAGLQGIPHEYYEAALIDGANP
                     VQAFIHVTLPFMKSAIQMSLFFCIIGSLQVFDVIWSMGQGGPVHAAETAVVYLYKFGI
                     KSSKMGFGSMVAIVIFCICLVFNVLYQRMINKEDR"
BASE COUNT          301 a          205 c          262 g          422 t
ORIGIN      
        1 gctgttgata gatcatatca ggcagagaag aagtaaaaaa ataagcggcg ggatttctcc
       61 tctcccgccg ctttccaggg ggtagtgatg gctgtcatga tgagcgcggt tacagaaagt
      121 aaaaagaatg ggacgaaaaa aggtgtggct aaaaatcatg agcgtgcgct tttttatgcc
      181 ttgcttttac ccgcatttat tctggttacc ttgtttataa tttatccggt agtaaataca
      241 atcttttatt ctttttatga ttggaatggg ttcggtccgt taaagaattt tgtagggctt
      301 aaaaatctac ggacattatt atccgatccc ttgttttgga atgcgcttcg gaataacggg
      361 attgttattc ttttttctat tggcatacaa cttccaatag cgttcctgct tgcgatactg
      421 gtaactcgaa gcactaatcg attaacgatt ttttttagaa cggttttctt tttaccgtat
      481 atcctttctg aaataattac cggagtaatt tggcgatttg tgtatcatcc acagtttgga
      541 ttgctacaac aagtgttcaa gtttttctct cctgagtatt catataccgg tttgcttgcc
      601 gatccaaaaa cggtttttat tgctattctt tttgttatct tttggaagta ttttgggttt
      661 catatgattt tgtatgtagc gggtcttcag ggtatccctc atgaatatta tgaagcagct
      721 cttattgacg gagctaatcc agtgcaagcg tttattcatg taaccttgcc ctttatgaaa
      781 tcggcgatac agatgtcctt atttttctgt attattggtt cacttcaagt ctttgatgtg
      841 atttggtcca tgggacaggg tggcccggta catgctgcgg aaaccgcggt tgtgtattta
      901 tataagtttg gcattaaatc ttcaaagatg ggctttggca gtatggtcgc tattgtgatc
      961 ttctgtatat gtcttgtatt taacgtgctc tatcagcgca tgataaacaa ggaagacaga
     1021 taatgattga acgaacagta aatataaaga agaacatgaa tcttgtacag ggaactcagt
     1081 atgcactatt gatacttttt gccctggtta ttctggtgcc gatttatctt gcggtaattg
     1141 gcggtcttaa aacgattggg cagcttaacg ctgatccggt aggttttcca
//
LOCUS       DAOQXQ010000618         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_157750_length_1186_cov_2.914235, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000618 DAOQXQ010000000
VERSION     DAOQXQ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157750_length_1186_cov_2.914235"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1186)
                     /locus_tag="PKH81_09075"
     CDS             complement(<1..>1186)
                     /locus_tag="PKH81_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98227.1"
                     /translation="TEKYYEDTNTIRTWKLETPAADGEWKIAGISLNDEDRSILAAGQ
                     DMRLIIRPSPASAINESDPLAIRLSTATIEITETEFTASLEPQQPNGSGTFVTERLDT
                     HNLAARKDEVLSKFISSSVNRVLAFGFTPHLHTISARLTRFFPEAPFDVYRTFAFYLY
                     PERLPEDDHALDSARMSVRLTRASTTGEKAALDISLSARALESGTWNLISINLDTNTV
                     QVNGRDIPASQVMVNARDRSVRPVRLDLIFTGWPTVPEDQLLTAYGSNAWGFIVDEWY
                     LEETEQEIIGRNQTTLAWKKEGPVVSALSTPVISNPSLSIRSDSATGTGVTESVVSTS
                     AEASVDILRAKTSGFVSASSASDRAIDSAGYTLSIPVGPVTASENYQADFGLQSVRRE
                     DSL"
BASE COUNT          290 a          267 c          317 g          312 t
ORIGIN      
        1 aagtgaatct tctcttcgca cagattgaag accaaaatct gcttgatagt tttctgatgc
       61 ggtaacaggg ccgactggaa tggacagcgt gtatcccgcg ctatctatcg cgcgatcaga
      121 ggcgcttgaa gcagagacaa agcctgatgt ctttgcgcgt aagatatcga cagatgcctc
      181 tgcgcttgtt gagacgacgc tttcggtgac tcctgttccg gtagcactat cagagcgtat
      241 tgaaagagat ggatttgata tgacaggagt ggagagcgcg ctaacgaccg gcccttcttt
      301 tttccatgca agcgttgttt gatttcgacc aattatttcc tgttcagttt cttcaagata
      361 ccactcgtcc acaataaagc cccaggcatt tgagccatag gcagttaaga gttggtcttc
      421 ggggacggtg ggccatccag taaagatcag atcaagacgg actggtcgaa cgcttctgtc
      481 tcgtgcgttg accataacct gggatgcggg aatgtcgcga ccgtttacct gaacagtgtt
      541 tgtatcaagg ttaatgctga tcaggttcca ggtcccgctt tctaaggcgc gggcactcaa
      601 cgatatgtca agggcggcct tttctccggt tgtactcgcg cgggtaagcc gtaccgacat
      661 gcgtgcagag tccagagcgt ggtcatcctc cggaagacgt tcagggtata gataaaatgc
      721 aaaggttcga tatacatcaa aaggagcttc ggggaagaaa cgagtgaggc gagcgctaat
      781 ggtatgtaag tgcggagtaa aaccaaaggc aagcacccgg tttaccgagg aagaaatgaa
      841 ttttgacagg acctcatctt tacgagccgc caggttatga gtatccagcc gttcggtaac
      901 aaaggttcca cttccgtttg gctgttgagg ttcaaggctt gcggtaaact ctgtttctgt
      961 tatttctatg gttgcagtgg acaggcgaat cgcaagaggg tcactctcgt taatagcaga
     1021 agcaggagaa ggacgaatta ttaaacgcat atcctgaccg gctgcaagta ttgaacgatc
     1081 ttcatcgtta agcgagattc ctgctatctt ccattcaccg tccgcggcag gtgtttccag
     1141 tttccaggtc cggatcgtat tagtgtcttc atagtatttc tctgtt
//
LOCUS       DAOQXQ010000619         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_158178_length_1184_cov_3.880425, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000619 DAOQXQ010000000
VERSION     DAOQXQ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158178_length_1184_cov_3.880425"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..609
                     /locus_tag="PKH81_09080"
     CDS             157..609
                     /locus_tag="PKH81_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ98228.1"
                     /translation="MARYKPMLTDEQWKKIEPLLPEFPRSTKGGPKPINNRDCLEGIL
                     WVLRSGARWKDLPDSYPSASTCWRRLRDWEECDVWLTLWRVFLAELDEQGKLDWEQIF
                     ADGSFAPAKKGASAWEKPNAERVRSGWWWQTARVFLSECDLPRPHRRK"
     gene            540..962
                     /locus_tag="PKH81_09085"
     CDS             540..962
                     /locus_tag="PKH81_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNQ98229.1"
                     /translation="MVVADGAGVPLGVRLTSATPAEVTLLEPTLEQVCVPRLRGGAPR
                     NKPCRVIADRAYDSDALRTRLKQRGIELICPHRRGRVRPPTQDGRALRRYRHRWIVER
                     TMAWLSNFRRLVVRYERHLTMYQAFFHVACVIITLRKL"
BASE COUNT          239 a          341 c          363 g          241 t
ORIGIN      
        1 cggcgtcttt ggcgtaccaa acaatgtagt cacccattcg ttcgaggtac ttcgcgccaa
       61 gtggcataga gccggtttca taaccgccca ttcgtaaatt tgcgccatac atgaggttgt
      121 ggtctactgg tccgtcgcaa gcgaaaggag ttcgcgatgg ccaggtacaa gccgatgctt
      181 acagacgagc aatggaagaa gatcgagccg ctgcttccgg agtttccgcg ttccaccaag
      241 ggcggtccca aaccgatcaa caaccgcgat tgcctcgaag gcattctttg ggtcctgcgc
      301 agcggcgcgc gatggaagga cctgcccgat tcgtatccgt cggccagtac gtgttggcga
      361 cgattgcgcg actgggagga atgcgatgtc tggttgacct tgtggcgcgt cttcctcgcg
      421 gaactggacg aacaaggcaa gctcgactgg gaacagattt tcgccgacgg cagcttcgct
      481 cctgcaaaaa aaggggcctc ggcgtgggaa aaaccaaacg cggaaagggt acgaagtgga
      541 tggtggtggc agacggcgcg ggtgttcctc tcggagtgcg acttacctcg gccacaccgg
      601 cggaagtaac gttgctggag ccgacgctcg aacaggtttg cgttccgcga cttcgcggcg
      661 gcgctccgcg aaataagccc tgtcgcgtga tcgccgaccg tgcgtacgac agcgacgcgc
      721 tgcggacgcg actgaaacaa cgcggcatcg aactgatttg cccgcaccgg cgcggtcgcg
      781 tcaggccgcc gacgcaagac ggccgcgcgc ttcggcgcta ccgccatcgc tggatcgtgg
      841 agcgcaccat ggcctggctc tccaacttcc gcagactggt cgttcgttac gaacgccatc
      901 tcaccatgta ccaggccttt ttccatgtcg cttgcgtcat cattacactg aggaagttat
      961 gaaaccggct ctaattgagt cgcggcaaca gcatagcatc agagggcatt gcgcttattg
     1021 tgggtatgcc ttaatcgttg ccggtcctgc atatagatgc ccggagtgcg gccatcgggc
     1081 tatcgcaggt gggcagatcc ccttgaaact cgcaccgccc ggctgggttc gcacgttatc
     1141 gctcggagca aagtcacttc aagtgacggg tggtcttgca gttg
//
LOCUS       DAOQXQ010000620         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_158416_length_1183_cov_3.054965, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000620 DAOQXQ010000000
VERSION     DAOQXQ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158416_length_1183_cov_3.054965"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKH81_09090"
     CDS             <1..291
                     /locus_tag="PKH81_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HNQ98230.1"
                     /translation="MVAYAWGGFGAAFGPLIILALCWKGVSWWGSLSGVLSGSVTIFV
                     VKNFVKIPGEYFYELMPGFIISFVITVLISLITKKPSKETLEVFDQTLSELA"
     gene            complement(344..>1183)
                     /locus_tag="PKH81_09095"
     CDS             complement(344..>1183)
                     /locus_tag="PKH81_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ98231.1"
                     /translation="AGNDDLCAHVQEIYQEALQNAQDKKLTISGLGIGLSGIVDPYNG
                     VILNSNPLSITSPWPIRTILEAKLGIPVFVENDANCCCWGELAFRSNGPSRNFLAFLG
                     EFRNVDIGKNKINGTAFGIGLVIRGNVFHGDTFTAGEFRSLLYDHTKPGNSQFSISDQ
                     EAMRLPGDTGVLERVFDEVAYNGSLLVNCLDMNKVVIAGDFAAYPELLQKKLEQAIQS
                     NWIYTTKKECAIEFSPDGPLAVAMGAAGLFIEKLFSVPDMTDHIDEAVGYILLERISP
                     SPT"
BASE COUNT          310 a          262 c          273 g          338 t
ORIGIN      
        1 atggtggcct acgcatgggg cggttttggt gcggcatttg gaccgttaat cattcttgcc
       61 ctttgctgga agggtgtctc ttggtgggga tcactttctg gggtcctttc aggctccgta
      121 actatttttg tagttaaaaa ctttgtaaaa attcctggcg agtattttta tgaattaatg
      181 ccaggcttta ttatcagttt tgtgattacc gttcttatca gtttgataac gaaaaaacct
      241 tctaaggaaa ctcttgaggt gtttgatcaa acactcagtg agcttgccta aaaagaaata
      301 caaaagaatc tgtgaatagg ggttcccgac gggggcccct tttttatgta ggtgatggag
      361 atattcgttc gagcaggata tatccaacgg cttcatcgat gtgatcggtc atgtcgggaa
      421 cgctaaacag tttttctata aagagcccgg cagctcccat tgcgacagca agcggaccat
      481 cgggagaaaa ctcgattgca cattcttttt tggttgtgta tatccagttg gactgtatag
      541 cttgttcaag tttcttttgc agtagttcag gatacgccgc aaaatctccg gcgatgacaa
      601 ccttattcat gtccaggcaa ttaaccaaca acgatccatt atatgcaacc tcgtcaaaaa
      661 cacgctcgag caccccggta tctccgggaa ggcgcatcgc ttcttggtcg ctgatggaaa
      721 actgggaatt tcccggcttg gtatggtcat acagaagact acgaaattct cctgcggtaa
      781 aggtgtctcc gtgaaagaca tttccgcgaa ttaccaagcc tatgccaaac gctgttccgt
      841 ttatcttatt cttgcctatg tcaacattac ggaactcccc caaaaaggca aggaaattac
      901 gcgaagggcc attgctgcga aacgcaagct caccccaaca acagcaattg gcgtcattct
      961 ccacaaaaac gggtataccg agctttgctt ccagaatggt acggatgggc catggtgatg
     1021 taatactaag gggatttgaa ttaagaatta caccgttgta gggatcaact attccagaaa
     1081 gaccgatacc cagaccgcta atggtaagtt ttttgtcttg agcgttctgt aaggcctctt
     1141 gatatatctc ttgcacatgc gcgcataaat catcgtttcc ggc
//
LOCUS       DAOQXQ010000621         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_159327_length_1179_cov_3.226868, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000621 DAOQXQ010000000
VERSION     DAOQXQ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159327_length_1179_cov_3.226868"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1179
                     /locus_tag="PKH81_09100"
     CDS             23..>1179
                     /locus_tag="PKH81_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ98232.1"
                     /translation="MKGEGETSSTKLISSYLDLSVVACESGTIIRATHESLDSLSSPW
                     LDGTLLSSPSVNGFHGALWELARGCPYSCSYCYESKGIKQVRYFPLSRLSAELHEFVR
                     MGIERVFVLDPTYNCSRERALQILALIEKIAPTIHFNFEVRCELLDREIVRAFSRIPC
                     SLQIGLQSSNPRALTAVSRPTDIKAFTKKIGLLNEAGIVFGLDLICGLPEDSLSTFRS
                     SIDFALSLYPNNLELFRLAVLPGTTLHDQAAFLSLRHQDTPPYHVLSLPSFPAADIDR
                     AMELARAVEFFYTHGRAVSWFLAVTYPLKIKSSRFFSDFAAFLQRQYPAMPLDQVAHR
                     DIEAIQLAFLELVYQEKGLSWAWPVVKDLVSLNGAWTRALADGESSMLDLS"
BASE COUNT          257 a          288 c          276 g          358 t
ORIGIN      
        1 gattatatta ggaataatac gagtgaaggg agagggcgaa acatcttcca caaaactgat
       61 aagctcatat cttgatcttt ctgtggtcgc ttgcgaatcg ggaactatta ttcgggcaac
      121 acatgaaagc cttgatagcc tttcatctcc ctggcttgat gggacccttt tgtcttctcc
      181 gtcagttaac ggctttcatg gcgccctgtg ggaacttgcc agggggtgcc cgtattcctg
      241 ttcgtattgc tatgaatcaa agggtatcaa gcaggtcaga tatttccccc tttcccgtct
      301 tagcgcagaa ttacatgagt ttgtacgcat gggaattgag cgggtttttg tgttggatcc
      361 aacgtataat tgctctcggg agagagcctt acagatattg gcccttatag aaaagatcgc
      421 gcccacgatt cattttaact ttgaggttcg gtgtgaatta cttgaccggg agattgtccg
      481 cgctttttca cggattccct gttcactgca aatcggattg caaagttcaa atccccgcgc
      541 ccttaccgct gtttcccgcc caactgatat aaaagcgttt actaaaaaga taggcctgtt
      601 aaatgaagcg ggtatagtgt ttggccttga tttaatctgt ggcttacctg aagacagtct
      661 gtccacattc cgctcgagca ttgattttgc cctgtccctg tatcccaata acctggagct
      721 gttccggctt gctgttctcc ctggaactac actacacgat caagccgcgt ttttgtcttt
      781 acggcatcaa gacacaccgc cgtaccatgt gctttctctt ccttcttttc ctgctgccga
      841 catcgacaga gccatggagc tcgctcgcgc cgtagaattt ttttacactc atggcagggc
      901 ggtttcctgg tttttagccg ttacatatcc gctaaagata aagagttccc gttttttctc
      961 ggattttgcc gcgtttctcc agcgtcagta tccagcgatg ccccttgatc aagtcgcaca
     1021 tcgagatata gaagccatac agcttgcctt tttggagctc gtataccagg agaagggcct
     1081 ttcttgggca tggcctgtgg taaaggattt ggtttctctc aatggtgcct ggacacgggc
     1141 tcttgccgat ggtgagtctt caatgctaga tctatcgta
//
LOCUS       DAOQXQ010000622         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_159788_length_1177_cov_3.180036, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000622 DAOQXQ010000000
VERSION     DAOQXQ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159788_length_1177_cov_3.180036"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1177)
                     /locus_tag="PKH81_09105"
     CDS             complement(<1..>1177)
                     /locus_tag="PKH81_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNQ98233.1"
                     /translation="IIGSMFLGVIEVLGVSTIMPFIAVASQPELIRTNEYLSWAYQTF
                     GFTSDKTFLLALGILLFAFVLLRNIYLALYTFAQIHYTSMRRHSLSFKLFRLYLSQRY
                     PFFLNKNSYDFVKNINIEIERMINGTIMQLVNIICYLFQIVLLTAFLFYINTFVTLSI
                     TVVLVSVYGLIYVLIKKEVHRLGIERYKMTSLLARIVNEAFWGIKETKITGCERSFIS
                     SYTGPSRTFSNNFTKEEVINVIPKYILEVVSFSSILALILIMLGKSDNFTEVISSISV
                     YAYAGYRLMPSVQSVFRSFNKFKYSMPAAQKIVQEFDYKHNEQDHIDNEENRLQFSSS
                     IELRNIAFAYENNKQKNVLSDVSLSITANTLVGFVGKTGSGKTTLVDILLGLLVPDQG
                     "
BASE COUNT          379 a          234 c          252 g          312 t
ORIGIN      
        1 ccctgatccg gtaccaaaag tccaagcaat atatcaacaa gtgtcgtctt tcccgatccg
       61 gttttaccga caaatccgac aagggtattc gccgttatgg aaagactcac atctgagaga
      121 acattttttt gtttgttgtt ttcataggcg aaagcgatat ttcgcagttc tatagatgag
      181 gaaaactgaa gtcgattctc ctcattatct atatggtctt gctcgttatg tttgtaatca
      241 aactcctgaa ctattttctg tgcggctggc atagagtact tgaacttgtt aaatgatctg
      301 aacacgcttt gcaccgaggg catgagccga tagccggcat aggcgtacac acttatcgat
      361 gaaataacct ccgtaaagtt atcgcttttc ccgagcatga taagtatcaa ggccagtatt
      421 gaactaaatg atacgacttc aagaatatat ttaggtatta cgttgataac ctcttccttg
      481 gtaaaattat tggaaaaggt tcgtgacgga cctgtgtatg aactaatgaa cgaccgctcg
      541 cagccggtaa tctttgtttc tttgataccc caaaaagcct cgttaacgat acgcgctaaa
      601 agcgaggtca tcttgtatcg ctcaatacca agacgatgga cttctttttt tataaggacg
      661 tatataaggc cataaaccga aacgagaacg accgttatgg agagggtaac gaaagtgttg
      721 atgtaaaaca aaaaggcagt taacagcact atctgaaaga gatagcaaat gatattaacc
      781 aattgcatga ttgtaccatt tatcatgcgc tctatctcaa tattaatatt cttgacaaaa
      841 tcatagctgt tcttgttcag gaaaaaagga tagcgctggg ataaataaag acgaaaaagc
      901 ttaaaggaga gtgaatgacg cctcatgctg gtataatgaa tctgcgcaaa ggtatacagg
      961 gcaagatata tatttcgtaa aaggacaaag gcgaacagca atatccccag cgcaagaaga
     1021 aaagtcttgt cgctggtaaa gccgaaggtc tggtaggccc aagaaagata ttcattcgtt
     1081 cgtatgagct ccggctggga tgccacggca ataaagggca taatggtact gaccccgaga
     1141 acctcaataa caccgagaaa catcgagcca ataatca
//
LOCUS       DAOQXQ010000623         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_160237_length_1175_cov_3.058036, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000623 DAOQXQ010000000
VERSION     DAOQXQ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160237_length_1175_cov_3.058036"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKH81_09110"
     CDS             complement(<1..854)
                     /locus_tag="PKH81_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ98234.1"
                     /translation="MTDILGIGNALLDIFCFSEEETALSLGIHPNSTNHVSPDRLDEL
                     LLGIRTPVLVSGGSASNALKAASSLGLQCSFIGCTGTIEGESDRWALVLGADLASYGV
                     HLALEDRSHTTGRCLIIHMPGALKSIVCAPGAAPSIRPEQITQEAVNNARIVMLDGQL
                     LRYSELSNHICTLASQAKVPLALDIASVDIAKNHSILLPGLLTRGKTILFCNKDEAEE
                     LALVLADTVPGDRGFQTREELVHSVFSFYTARKRSFPCIVRKEGPLGATAWCSGSVIH
                     REGQAIDLP"
     gene            complement(870..>1175)
                     /locus_tag="PKH81_09115"
     CDS             complement(870..>1175)
                     /locus_tag="PKH81_09115"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700355.1"
                     /note="involved in coenzyme B(12)-dependent 1,
                     2-propanediol degradation; important for the synthesis of
                     propionyl coenzyme A during growth on 1,2-propanediol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HNQ98235.1"
                     /translation="HLGIKLDLAKNELARTRNAETCISADDSQIKIFVIPTDEELVMT
                     EDAYALMKGTYDVHTKFTYSFQSPDYVNKGREEGLIRDLEKNPGLRDIIVKIPGTKK"
BASE COUNT          262 a          279 c          337 g          297 t
ORIGIN      
        1 ggcaggtcta tcgcttgtcc ttcccggtga ataacacttc ccgaacacca ggccgtagct
       61 ccaagcgggc cttcctttct gacgatacag gggaagcttc gttttcgggc ggtataaaac
      121 gaaaacacgg aatggaccag ttcttctctt gtttgaaagc cgcgatcccc gggaacggta
      181 tcggcgagca ccagggctaa ttcttctgcc tcgtctttgt tgcaaaagag gatggttttg
      241 cctcgggtaa gtaagccggg cagcagaatg ctgtggtttt tagcgatatc aaccgaggca
      301 atatcaaggg caaggggaac ctttgcctgg gacgcaaggg tacaaatatg atttgaaagc
      361 tcactatagc gcaacagttg tccgtcgagc ataacgatgc gggcgttgtt aactgcttcc
      421 tgggttattt gttcaggtct gatggacggg gcggctccgg gggcgcagac gatggatttt
      481 agcgcgccgg gcatgtgaat aatgaggcat cggccggtgg tgtgcgagcg gtcttccagg
      541 gcaaggtgta ccccgtacga ggcaaggtct gcacccaaga cgagggccca tcggtcgctt
      601 tccccttcga tcgttccggt gcatccgata aatgagcact gtaagcccag agaggaggct
      661 gcctttaggg cgttagatgc tgaaccgcca gaaaccagga caggggtacg aattcccaag
      721 aggagttcgt ccagcctgtc cggtgaaaca tggtttgtgc tgtttggatg gatacccaga
      781 gagagggctg tttcctcttc cgagaagcaa aagatatcaa gcagggcgtt tcctattccc
      841 agaatatccg tcactggcgc tccttggttc tattttttgg tacctggtat cttgacaata
      901 atatcacgca aacccggatt tttctcgaga tcacggataa gaccttcttc ccggcccttg
      961 tttacatagt cgggagactg gaaggagtag gtaaacttgg tatgaacgtc gtaggttccc
     1021 ttcatcaggg cataggcatc ttcggtcata accagctctt cgtcggtggg gattacaaag
     1081 atcttgatct gtgaatcgtc ggcgctgatg caggtttctg cgtttcgggt tctggcaagt
     1141 tcattctttg caagatcgag cttgataccc agatg
//
LOCUS       DAOQXQ010000624         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_160754_length_1173_cov_2.048301, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000624 DAOQXQ010000000
VERSION     DAOQXQ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160754_length_1173_cov_2.048301"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKH81_09120"
     CDS             complement(<1..749)
                     /locus_tag="PKH81_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ98236.1"
                     /translation="MSISSTALLISYILNIASIFFILFFERSTPARRFTWILAIAFIP
                     GLGVLLYILLSGSFFTRTKRMAEATSRANARYAHILKSQHTNLEELVKTGTHPGISEI
                     SSLIHMNLVHGKSPVTIHNEVTILTSGQEKYPMLFEEIQLAEKSIHLSYFIFKNDVIG
                     RHLVSLLAERAARGVQVRLLYDHVGSISTSPRLFLPLIQAGGQVSRFFPVSPFNLFPL
                     NYRNHRKIVVIDGNIGYFGGMNIGDEYANAST"
     gene            complement(769..>1173)
                     /locus_tag="PKH81_09125"
     CDS             complement(769..>1173)
                     /locus_tag="PKH81_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98237.1"
                     /translation="LKSACAFGYDSSEGLSLVPTLELRLRRLAATVALRGVWGNQYGS
                     LDKGEGRAQAGFSLRSIGSIMSAGIWVLGGTDSLYQKPEDINLTARLSSGLSFQCILP
                     STNLLISTEASWERDGWGNEGIACRAGFGLIF"
BASE COUNT          324 a          268 c          292 g          289 t
ORIGIN      
        1 gtgcttgcat tcgcgtactc atccccgata ttcataccgc cgaaataacc aatgttcccg
       61 tcgataacca caatcttgcg gtggttgcga taattaaggg gaaacaggtt aaaaggagaa
      121 acagggaaaa aacggcttac ctgacctccc gcctggataa ggggcaggaa aagacgggga
      181 gaggtgctga ttgaaccaac gtgatcatag agtaacctga cctgaacgcc tctagcggct
      241 cgctcggcaa gaagagaaac cagatgcctt ccgataacat cgtttttaaa aataaaatac
      301 gagaggtgaa tgctcttttc ggcaagctgt atttcctcaa agagcatggg atacttttct
      361 tgtccggatg tcagaatagt aacctcgttg tggatggtta ccggactttt accatggact
      421 agattcatgt ggataagcga gcttatttcg ctaattcccg gatgagttcc tgtctttacc
      481 agttcttcca gattagtgtg ctgactttta aggatgtgtg catatcgtgc gtttgcacgg
      541 gatgtggcct cggccatccg ttttgttcgg gtaaaaaacg atccggaaag caggatgtat
      601 aacagaaccc caaggccggg aataaaggct atggccaata tccaggtaaa gcggcgggca
      661 ggggttgagc gttcaaaaaa aaggataaag aaaatcgacg cgatgttaag aatatacgag
      721 ataagcaggg ctgtagagga gatactcatc gttttttttc ctttcggttc agaaaattag
      781 tccaaatccc gcacgacatg ctattccctc gtttccccag ccatcacgtt cccaggaagc
      841 ttccgtagaa atcaagagat tggttgacgg aagtatacac tggaatgaaa gtccgcttga
      901 aaggcgggct gtaaggttga tatcttctgg tttttgatac aaggaatccg ttccccccag
      961 aacccatata cccgcgctca tgatcgatcc tatggatcta agagaaaatc cggcctgtgc
     1021 cctgccttct cctttatcaa gtgatccata ttgattcccc cagacacctc gcagggcgac
     1081 tgttgccgca agacggcgca accggagttc aagggtcggt acaagagaaa gtccttcgct
     1141 actatcgtag ccaaaggcgc aggcgctttt aag
//
LOCUS       DAOQXQ010000625         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_161847_length_1168_cov_2.707996, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000625 DAOQXQ010000000
VERSION     DAOQXQ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161847_length_1168_cov_2.707996"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKH81_09130"
     CDS             <1..715
                     /locus_tag="PKH81_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98238.1"
                     /translation="LIQDLYFKRLAFFVEKAGYRGTLVSDSDMQGKHGFNAHDYRAES
                     LAQFFEKARRENFQLNREELELRDILLAEGIIQNGSDGYTEGSGAVLSLSRQSAEYLR
                     YLFLTHECFHGLYFTDPDFRSQVSQIYHATDERARIFLQTYFSLMDSLSYDLDDEYLM
                     ENEFMAYLLQQPVDKVGDYFTGVISERFLRYGGESEQVDYIKESDAIDFIEAATELEA
                     YVFRRWGLAAGRAGLWFSK"
     gene            complement(708..>1168)
                     /locus_tag="PKH81_09135"
     CDS             complement(708..>1168)
                     /locus_tag="PKH81_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98239.1"
                     /translation="HNHETHIKGLQTLLRIYTPKVYSADAELKGIKTVLLQGDGTFVA
                     AGFAVRYFAAPGHSPDSLMYQIEHVIFTGDALSAGRLGVTNNAYGKRNLLTHLRNKLL
                     NQNDDILLMSGHGPPSTIGAERLFNSELMHSGTDDHDILREILRSPSILT"
BASE COUNT          276 a          250 c          303 g          339 t
ORIGIN      
        1 tttgatacag gatctctatt ttaagcggct cgccttcttt gtggaaaagg ccggatatcg
       61 ggggaccctt gtttcggatt ctgatatgca aggtaaacat gggtttaacg ctcacgatta
      121 tcgcgcagaa agccttgccc aattttttga aaaagcgcgc agggagaact ttcagctcaa
      181 tcgagaggag cttgaactcc gtgacattct tcttgccgaa gggattatcc aaaatggaag
      241 cgacgggtat accgaaggct ctggagccgt tctgtcactc tctcgccagt ctgccgagta
      301 cctacgctat ctttttttga ctcacgaatg ttttcatggg ttgtatttta ctgatcctga
      361 ttttcgctcc caggtatcgc agatttatca cgctaccgat gagcgagctc gaattttttt
      421 gcagacctat ttttcattga tggatagcct ttcctacgat cttgatgacg agtatctaat
      481 ggaaaacgag tttatggcat atctgttgca acagccggtt gacaaggttg gagattattt
      541 taccggtgtt atcagcgagc gatttcttcg ttatgggggg gagagcgagc aggttgacta
      601 tattaaggag agcgatgcca tcgattttat agaggccgca acagaactgg aagcatatgt
      661 ttttcgcaga tggggccttg ccgccggcag ggcgggactg tggttttcta agtaagaatg
      721 gatggcgagc gcaaaatttc tctgaggata tcatgatcgt ccgtacccga gtgcatcaat
      781 tcgctgttaa aaaggcgttc ggcgccaatg gtggaaggcg gtccatgccc gctcatcagt
      841 agtatatcgt cgttttggtt gagcagtttg tttcgtaaat gtgttaaaag atttcttttt
      901 ccgtacgcgt tatttgttac cccaaggcga cctgccgaaa gggcgtctcc ggtaaagata
      961 acatgttcaa tctgatacat aagggaatcc ggggaatgtc cgggggcagc aaagtagcga
     1021 actgcaaagc ccgctgcgac aaaggttcca tccccctgca acaatacggt tttgataccc
     1081 ttaagctccg catctgctga gtataccttt ggggtataga ttcgtagcag cgtttgcaat
     1141 ccctttatat gcgtttcgtg attgtgcg
//
LOCUS       DAOQXQ010000626         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_161860_length_1168_cov_2.548068, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000626 DAOQXQ010000000
VERSION     DAOQXQ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161860_length_1168_cov_2.548068"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1168
                     /locus_tag="PKH81_09140"
     CDS             160..>1168
                     /locus_tag="PKH81_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ98240.1"
                     /translation="MSRTFFTLGASLSLAITAAVALSFILPAGAALIPLFAHQGMPLT
                     FDHLRALVSALMFTATQALLSSLVAILVGIPAAFLVARREFPLRRLLLSLSGIPLCVP
                     PVIVAFAFVLFYGRQGYLNTVLQALTGAVDPPITFLYSLWGVVLAHGLYNFPVVLRAV
                     SQIWERTDSCTEEAAALLGAGRLRILRTVVFPQLAGAVFSSGALVFLYCFFSFVIVLL
                     FGGIGGTSLEVELFQAARSDLNFRYAALVALTETLAGLGIVLAYVRIEGQLSSRSSRA
                     PQLRPRHRLHGTGELCLSIAYLLSIAVFFLGPLVSVVCRSLSIVPSGTISLSLLRAPW
                     QT"
BASE COUNT          181 a          311 c          295 g          381 t
ORIGIN      
        1 gtatccggtg tctccctcgg ttactctccc ttcctcctat gtggccgctc cgcacgccgc
       61 aaagaaattg tttgtagatc cggcctctct tgctccagct acgcgcaaaa tgctgtctat
      121 tttgggtaat tgatgagagg ggaatgtgag cgaaaggtga tgtctcggac tttttttacc
      181 ctgggagcaa gcctgtcgct tgcaataacc gcagctgttg ccctttcgtt tatacttccc
      241 gcaggcgcgg ccctgattcc cttgtttgcg catcagggga tgcctcttac attcgatcat
      301 ctgcgcgccc tggtatctgc cctgatgttt acagcgaccc aggccttgct ttcgtcgttg
      361 gttgccatcc ttgtaggaat acccgcagct tttttggtag cccggcgtga gtttccgcta
      421 agacgattgc tgttatccct ttccggcatt cccctgtgtg tgccgccggt tattgtcgca
      481 tttgcctttg ttctttttta tggcagacag ggttacctga ataccgtact acaggctctt
      541 accggcgctg ttgatccccc cataaccttt ttatattctt tgtggggggt tgtattggct
      601 catggattat acaattttcc tgtagtactg cgagcggtga gccagatctg ggagcgcact
      661 gactcctgta ccgaagaggc agccgctcta ttaggagcgg gaaggcttcg tatattgcga
      721 acagtggtgt ttcctcagct cgccggggcc gtattctctt cgggggctct tgtcttttta
      781 tattgctttt ttagttttgt tatcgtgctt ttatttggcg gaattggagg cacctcgctg
      841 gaggttgagt tgttccaggc cgctcgctct gatcttaatt ttagatatgc ggccctggtt
      901 gccttaacag aaacccttgc aggacttggc attgtattgg cctatgtgcg gatagaaggt
      961 cagctatctt cccgctcttc tcgagcccca cagctgcgcc ctcgccatcg cctgcatggt
     1021 actggcgagt tgtgtttatc tatcgcgtat ctcctctcca tcgcggtgtt ttttttgggg
     1081 cctcttgtct cggttgtttg tagatccctg tctattgttc cttcgggaac catctctctt
     1141 tctctcttgc gggcaccctg gcaaaccc
//
LOCUS       DAOQXQ010000627         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_162200_length_1167_cov_1.625000, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000627 DAOQXQ010000000
VERSION     DAOQXQ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162200_length_1167_cov_1.625000"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1167
                     /gene="lepB"
                     /locus_tag="PKH81_09145"
     CDS             35..>1167
                     /gene="lepB"
                     /locus_tag="PKH81_09145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ98241.1"
                     /translation="MTILFTVCAAVSAAFALSSFLPGSAAALVSLSLSFATALAFFIA
                     TRRLIKTPSRMSLSLVRKLSEYVPFILFTAFIIRRSGDREGSFALDLVSALLWVAALV
                     SSLIVQWRLADKRLERFLPSCASLPASAVRKKTIITHLLEWADALLQAGCLVLLINLF
                     IFQMYAIPSESMVPEFMIGDRVVVLKTPSGPKFPLSEVGIPRMRDYHRGDIVVFSNPH
                     YNDTRQDRIRSFASQLVYMLTFTAVNINLDEYGAVKADPLVKRVCGVAGEKLMMVDGV
                     LYARTEGMDDYRPVSEDASWATWDIASLPRSQLALVKNVPLSKEAFDLVTSVESKRAN
                     LDLEAAKTDAIALANRFDLLKPSRDSAFLPEEIFSPARMELLSL"
BASE COUNT          235 a          299 c          268 g          365 t
ORIGIN      
        1 tttttgctat actaattttc tgtttggagg ttccatgacc attctcttta ctgtctgtgc
       61 cgccgtgtcg gcagcctttg cattaagctc ttttttacct ggctccgctg ccgcccttgt
      121 ctctcttagc ctatcctttg ctactgccct tgcttttttt atcgcgacca gacgacttat
      181 aaaaaccccc tcgcgtatga gtctttcgct tgtcagaaag ctttccgagt atgtaccgtt
      241 tatcctcttt accgccttta ttattcgccg ctcgggagac agggaaggct cgttcgctct
      301 tgacctggtg tccgcccttt tatgggtggc cgcgttggta agttccctga tcgttcaatg
      361 gcgccttgca gataaacgtc ttgaacgatt tcttccttcc tgcgcatcct tacccgcatc
      421 tgcagtacga aaaaaaacga tcattactca tcttcttgaa tgggcagacg ctctgttaca
      481 ggcgggctgt cttgttcttc ttattaatct ctttattttt cagatgtatg cgattccctc
      541 tgaatcaatg gttcccgagt ttatgatcgg agatcgtgtt gtagtattaa agacaccatc
      601 gggacccaag tttccattga gcgaggttgg tattccccga atgagagact atcatcgcgg
      661 ggacatcgtg gttttttcta acccgcatta taacgatacc cggcaggacc gtatccggtc
      721 ctttgcatcc cagttagtgt atatgcttac ctttaccgcg gttaatatca atcttgatga
      781 atatggcgca gtaaaggcgg acccgctggt aaaacgggtg tgcggggttg caggcgaaaa
      841 actgatgatg gtcgacgggg tcttgtatgc acgcacagag ggtatggacg actatcgacc
      901 agtgtcagaa gacgcttcct gggctacctg ggatattgcc tccctgccca gatcccagct
      961 tgccctggtt aagaacgttc cactctctaa agaggccttt gatttggtta cttccgttga
     1021 atcaaaaaga gctaatcttg accttgaggc ggcaaagacc gacgcgattg cccttgctaa
     1081 tcggtttgac ctgcttaaac cttccaggga ttctgctttt cttcccgagg aaatctttag
     1141 tcccgctcgc atggagttgc tttctct
//
LOCUS       DAOQXQ010000628         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_162541_length_1165_cov_2.693694, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000628 DAOQXQ010000000
VERSION     DAOQXQ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162541_length_1165_cov_2.693694"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKH81_09150"
     CDS             complement(<1..413)
                     /locus_tag="PKH81_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNQ98242.1"
                     /translation="MLYYLTSMIQQYFGPLRLLQSYAILIIIALYAGFFLTVRLLPRV
                     YRFLPKDRGREFSVNADAAMGKPTGAGAFFITLFVLVSFVFTPVTLPQAVTLLLTWLV
                     MCTGYLDDRSIHPWGEYLKGALDLALSLGASVALL"
     gene            complement(501..>1165)
                     /locus_tag="PKH81_09155"
     CDS             complement(501..>1165)
                     /locus_tag="PKH81_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ98243.1"
                     /translation="FGGIVGCNREIDGEAAEEMSKTFLEVVVAPSFSTEALEVFSKKK
                     NLRLIKADIVPASRIESLSVDGGVLVQERDAQLFSEWNVVTERAPTAAQAAEMAFGMT
                     IALFVKSNAIVVCKDRIALGIAGGQVNRIWPTDQALSRAKGLTDAGIPCLDGTTSTES
                     ASVMISDAFFPFADCVETAAKYGIQAIVQPGGSMRDQESIDAANRLGIAMVFTGTRHF
                     KH"
BASE COUNT          313 a          285 c          297 g          270 t
ORIGIN      
        1 aacaacaggg caacagatgc gcccagggac agggctaaat caagtgctcc tttaaggtat
       61 tcgccccagg gatggataga ccgatcatca aggtatcctg tacacatcac cagccaggtt
      121 aaaagcaggg ttaccgcttg cgggagggta acgggagtaa agacaaacga aacaagaaca
      181 aacagggtaa taaaaaaagc tcccgcgccg gtcggctttc ccattgcagc gtctgcgtta
      241 acggaaaatt ccctgcctct atccttgggc aaaaaacggt acacgcgagg taagagcctg
      301 acggttaaaa aaaagcccgc atacagggct ataataatca atatggcgta agactgcagt
      361 aggcgtaagg ggccaaaata ctgctggatc attgatgtaa ggtaatacag catggagcat
      421 tgtagaccta aaatacctga ttggacaatc cccgtcgttt tggcaaaacg atggggatcg
      481 gatcaaagcg ttgcttttta ttagtgttta aagtgtcggg tcccggtaaa gaccatcgca
      541 atgcccaaac gatttgctgc gtcgattgat tcctggtcgc gcattgagcc acctggttgg
      601 acgatcgctt gtataccata ctttgccgcg gtttccacac aatcggcaaa ggggaagaag
      661 gcatcagata tcatgacgga agcgctctct gtggaggttg ttccgtcaag gcaaggaatg
      721 cctgcatctg taaggccctt ggctcgggat agtgcttggt ctgtaggcca aatccggttc
      781 acctgtccac cggcaattcc cagggcaata cggtctttgc agactacaat cgcgttcgat
      841 tttacaaaga gcgcaatagt catgccaaag gccatctccg cggcttgtgc cgctgtgggc
      901 gctcgctctg ttacgacgtt ccactcgctg aataattgag catcgcgctc ttgcacaagg
      961 acgccaccgt ccacactcaa ggattctatt cgcgaggcag ggactatatc tgccttgatg
     1021 agtctcagat ttttcttctt ggagaatact tcaagagcct ctgtggaaaa cgagggagcg
     1081 acaacaactt ccaaaaaggt tttggacatt tcttcagcgg cttctccgtc aatttcgcgg
     1141 ttacatccaa cgattccgcc gaata
//
LOCUS       DAOQXQ010000629         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_164637_length_1156_cov_2.923706, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000629 DAOQXQ010000000
VERSION     DAOQXQ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164637_length_1156_cov_2.923706"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..871)
                     /gene="surE"
                     /locus_tag="PKH81_09160"
     CDS             complement(134..871)
                     /gene="surE"
                     /locus_tag="PKH81_09160"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNQ98244.1"
                     /translation="MTILITNDDGLSSEGIQILAAHLRDTHEVWVVAPSSNRSGVSHG
                     ITMGGSLELTRCGHQEYSCSGLPVDCSITAFEAIMPRRPDAVISGINRGANIGTDIVF
                     SGTAAAARQASFSGIPGIAVSLVCEDERFHWEPLARFVRDNLDTLVSLCDRDIFVNIN
                     APSLPRYRGARITGISRRLYKDSIRLETGNDGQTRSFFEGGTIDTHGDDDCDWRAVSS
                     GFVSVSRVHAQPLSASLGEIAEPAFKV"
     gene            complement(885..>1156)
                     /locus_tag="PKH81_09165"
     CDS             complement(885..>1156)
                     /locus_tag="PKH81_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNQ98245.1"
                     /translation="SHEGLRDRFEISCPELDWLVKRALEFVIPDVPELVCSRMTGRGF
                     GGCTYAILPKIHVDAYLKKLEDYERIFGFKPVQYQVCASDGACVL"
BASE COUNT          335 a          316 c          271 g          234 t
ORIGIN      
        1 aataggccag ctgaaggttt ccccctccct ctccggaagg aattcctaaa aaaaccgtaa
       61 gcgcgtctgt ccattgcccg ctacggtaca ggttaatccc ctctttcatc gtatccgaca
      121 tcatccctcc acattacacc ttaaaagccg gttcagctat ctccccaagg gaagccgaca
      181 ggggctgagc atgaactcgc gataccgaca caaagccgga ggatacagcc ctccagtcac
      241 aatcgtcatc cccatgggta tcaatagttc caccttcaaa aaagcttctt gtttgaccgt
      301 cgttgccggt ttccaacctg atactatcct tgtaaagccg gcgcgatata ccggtaattc
      361 gcgcgccccg gtaccttggt aaagacggag cgtttatatt aacaaagata tcccgatcgc
      421 ataaggacac aagggtgtca agattatccc gcacaaaacg tgcaaggggc tcccaatgaa
      481 accgctcatc ctcgcagaca agactgacgg cgataccggg aataccggaa aaagacgcct
      541 gacgagcggc ggcagcggtg ccggaaaata caatatcggt tccgatattt gcgcctcgat
      601 tgataccgga aataaccgca tcgggacgcc ggggcattat cgcttcaaag gcagtaattg
      661 aacagtcaac gggaaggccc gaacaggagt attcctgatg tccgcaacga gttagctcaa
      721 gggaaccgcc catggtaata ccatgggata caccggagcg attagaagaa ggggccacaa
      781 cccacacctc atgagtatcg cgtaaatgag cggctaatat ctgaattccc tcggatgaaa
      841 gaccatcgtc gttagtaata agaatcgtca tgaaaggcag tatatcataa aacgcacgcg
      901 ccgtcagagg cgcatacctg atactgaacg ggtttaaagc caaagatacg ctcgtaatcc
      961 tccaattttt taagataggc atctacatgt atcttgggca gtatggcata ggtacagcca
     1021 ccaaagccgc ggccagtcat tcgcgagcat accagctccg ggacatccgg aataacaaat
     1081 tcaagagcgc gttttacaag ccaatcaagc tcagggcagg atatctcaaa gcgatcccgt
     1141 aagccctcat gagagc
//
LOCUS       DAOQXQ010000630         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_164664_length_1156_cov_2.645777, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000630 DAOQXQ010000000
VERSION     DAOQXQ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164664_length_1156_cov_2.645777"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1082)
                     /locus_tag="PKH81_09170"
     CDS             complement(177..1082)
                     /locus_tag="PKH81_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98246.1"
                     /translation="MWYSPNGPVSYYYPIENYHYDDDSTHCPFFIRHAVASEDGNILL
                     GFGGGPDVRDPSHLPKMSVDGGKSWTKITNTDIPGAGWTRAVGISPDGTIQCILTRYF
                     TLYIRKNNMRWKKIKIPAEPKDFHTDGILEVTNSGTLITTGNYGKLYISKDLGKTWKT
                     EGLGSHKLAITKDGKSIIALLYLGYGNADDQSILMRSDDLGDSWTTIATFKERIQRFL
                     ISEDAQVLVAQNSDRTIISSRDSGLTWKTLLELDKDDFISAFFLSTEDNLTIKVLAYT
                     DYIEYLSVDGGLTFTKESILKKIQG"
BASE COUNT          269 a          263 c          253 g          371 t
ORIGIN      
        1 ccacggccgt aattcgcctt gctgttttct tcagtaattc atcgcgggta tacagtagta
       61 gatgattccc ctgtgcgtat tctgcaagaa agcggtgcag ggccggatta cgacatcccc
      121 tttctttacc ggggagttca tcattccgcc ttcaagaacg gtgtcttctt ccatatctat
      181 ccctgtatct ttttgagtat tgattctttt gtaaaggtta atccgccatc cacgctcagg
      241 tattctatat aatctgtata ggctaagacc tttatagtca gattatcttc cgtcgaaagg
      301 aagaaagcac tgatgaaatc gtctttatct aattccagta gtgtcttcca cgtaagcccg
      361 gaatctctgc tggagataat cgttcggtcg ctattttggg caactaatac ttgtgcgtct
      421 tcgctaatca ggaatctttg tatcctctct ttaaaagttg caattgttgt ccaggaatcc
      481 ccaagatcat cggagcgcat caagatgctc tgatcatctg cgtttccata gccgagatac
      541 aaaagtgcga taatactttt tccgtctttg gtaatcgcaa gtttgtggga acctagccct
      601 tcagttttcc acgtttttcc cagatccttg ctgatataga gctttccata gttaccggtg
      661 gtaattagcg tgcctgaatt ggttacctca agaatgccgt ctgtatgaaa gtcttttggt
      721 tctgcgggta tcttgatttt tttccagcgc atattgtttt ttctgatgta gagggtgaaa
      781 tacctcgtga gtatgcattg tatcgttccg tctggggata tgccaactgc tctcgtccat
      841 ccagctcccg gaatatcagt attcgttatc tttgtccagc ttttcccgcc atctacgctc
      901 atctttggca aatggctcgg gtcccgcaca tcagggcctc cgccaaaccc aagaaggatg
      961 ttgccatcct ctgatgctac ggcgtggcga ataaaaaagg ggcaatgggt tgaatcatcg
     1021 tcgtaatgat aattttctat tgggtaatag tagctaactg ggccgttggg ggagtaccac
     1081 ataagaaatg aatagtatca ttgacagccg atgaaagcaa taattactgc tcgcaggatt
     1141 gttggtattt tcctcc
//
LOCUS       DAOQXQ010000631         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_164927_length_1155_cov_2.461818, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000631 DAOQXQ010000000
VERSION     DAOQXQ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164927_length_1155_cov_2.461818"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..869)
                     /locus_tag="PKH81_09175"
     CDS             complement(9..869)
                     /locus_tag="PKH81_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ98247.1"
                     /translation="MTLALFIDSLTGISGYQEAVWLGVTDGATQAGVRIITFAGGALD
                     FSPLNPFEKSRNIAYELFSPTTASGLLLCGGTLGNDCSRETFSAFCSRYSVVPTVSVG
                     PAVPGIPRVLVDNDRGMKEMVNHLIRDHGYTRIGFISGPKGNIDADRRRKMYQEALTE
                     NSLIYEEAIVYEGDFNDHSGYEAVLHWISRGGAMPQAIVASNDNMAFGAINALREKGL
                     SVPHQIAVTGFDDVSSAATATPPLSTVSQPIYLQGKKAVELLIQRIKGGTIPEETLVL
                     SGRATWIYRL"
     gene            complement(949..>1155)
                     /locus_tag="PKH81_09180"
     CDS             complement(949..>1155)
                     /locus_tag="PKH81_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNQ98248.1"
                     /translation="GVPWFEYQETVRVKVFYEDTASIVQRSMMYRVAGVRSVSFWRLG
                     QEDPLIWKELSVKVPRLASQQTEN"
BASE COUNT          291 a          303 c          273 g          288 t
ORIGIN      
        1 atgtttgctc aaagacgata tatccaagtc gctcttcccg ataatacaag ggtttcctcg
       61 ggtattgttc cgcccttgat tctctggatc agtaactcaa ctgctttttt cccttgtaga
      121 taaatgggtt ggctcaccgt ggaaagaggc ggggttgccg tggccgcgga cgaaacatcg
      181 tcaaaaccgg taacggctat ttgatgtgga acggacaggc ctttttcccg taatgcgtta
      241 atcgcgccaa aggccatgtt gtcgttcgag gcgactattg cctggggcat cgctcctccc
      301 ctggaaatcc aatgcagtac cgcctcgtac cctgagtggt cgttaaaatc accttcatat
      361 actatcgcct cttcatatat caaggaattt tctgttaggg cttcttgata cattttgcgg
      421 cggcgatctg cgtcgatatt tcccttgggt ccactgataa acccaatgcg ggtatatccg
      481 tgatcgcgta tcaggtgatt gaccatctcc ttcatgcccc gatcattatc aactaaaacg
      541 cgggggatac cagggacggc aggaccaacg ctcaccgtcg gcactacgga ataacgagag
      601 caaaatgcgc tgaaggtttc gcgggaacag tcgtttccca gggttcctcc acacaaaagc
      661 aagccacttg ctgttgtggg agaaaacaac tcataggcga tgtttcggga tttttcaaag
      721 ggattcaggg gggaaaaatc cagggcacct ccggcgaagg tgattattct gacgccagct
      781 tgtgttgccc catcggtaac accaagccag acagcttctt gatacccgct aatcccggta
      841 agggaatcaa taaaaagcgc tagggtcata taaagcagtc ctccggttgt atatacacag
      901 atataccctg aatctacaaa aagcgctagt ccaaaatcac caatatctct aattttccgt
      961 ttgttgggaa gccaggcggg ggactttaac ggacaattct ttccatataa ggggatcctc
     1021 ctgtccgagt ctccaaaacg acaccgagcg cacgcccgcc acgcggtaca tcatgctccg
     1081 ctgtacgatt gaggcggtat cttcataaaa caccttaacc cgcaccgttt cttgatactc
     1141 aaaccaggga actcc
//
LOCUS       DAOQXQ010000632         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_165341_length_1153_cov_2.820583, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000632 DAOQXQ010000000
VERSION     DAOQXQ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165341_length_1153_cov_2.820583"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1025
                     /locus_tag="PKH81_09185"
     CDS             423..1025
                     /locus_tag="PKH81_09185"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNQ98249.1"
                     /translation="MSLSIERRILYRIRAKGRGWVFSARHFADFGSRSGIDLALHRLS
                     KQGKIRRLGQGLYDYPRIHEKIGVLSPSIEAVAREIADKNNSKLKIVGSQAANALGIT
                     TQVPAKIVFLTDGPTRQIKIGNQTIQLKHASPKVMTTADNGSGVVIEALKYVGRENID
                     ERTIRKIKGFLSEFDKKELQKNLNAVPEWMKPRLLELITD"
BASE COUNT          346 a          232 c          299 g          276 t
ORIGIN      
        1 cgtatccgaa agatcattgg cggtccaagg aaggagcgca tggatgcgcg ttccgcggat
       61 caaaatccag ccttcgtcga tcaccggccg cgcggggtcg ttcgtataga tccggacgtt
      121 gtggacaagg gtatccatga atcgcgggag caatcggctt caagtccgac cggcggacga
      181 agagttaagt gcaactccac tgcgaattta gcagataccg ccccgaatcg gaaaggttag
      241 tgtgaggttg tagagggtgg aattccgcaa attttctgtt tctatcattt tcagctttcg
      301 agttcactta aggattcagg atagagtatt ccttttctgt accttcaaga ttattaagat
      361 ttaggacttt tttatttgta agaaaaatgt agtatatttt cttacaagat ggtgatacct
      421 gtatgtcgct aagtattgaa cgaagaatac tttatagaat tagagccaaa ggaagaggtt
      481 gggtgttttc agccagacac ttcgcagact ttggtagcag aagcggcatt gatctggcac
      541 ttcatcgtct gtcgaaacaa ggtaagatcc gacggttagg gcaaggacta tacgattacc
      601 caagaatcca cgagaaaatc ggcgtgctga gtccgtcaat cgaggcggtt gcacgagaaa
      661 tcgcagataa gaacaacagt aaactgaaga ttgtcggctc tcaggctgca aatgctttgg
      721 gtataacaac tcaggtgccg gcgaagattg ttttcttgac agacgggcca acacggcaga
      781 taaagatcgg taatcaaaca atacagctca agcatgcttc accaaaggta atgacgaccg
      841 cggataacgg aagcggtgtc gttatcgaag cgctcaaata cgttggacgc gaaaatattg
      901 atgaacgcac gattaggaaa attaaggggt ttctatcaga atttgacaag aaggaactgc
      961 aaaagaatct caacgccgtc ccggaatgga tgaagccaag gttactcgaa ttgataacag
     1021 actgagttta tggacaaatt tgcgagaacc tcgcgcgaaa accgacaatt ggttttccag
     1081 gaaaccgcca gcaggaaagg gacaagtccg caaatcgccg aaaaagattt ctgggtatgt
     1141 tggactctta aga
//
LOCUS       DAOQXQ010000633         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_166360_length_1149_cov_3.305302, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000633 DAOQXQ010000000
VERSION     DAOQXQ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166360_length_1149_cov_3.305302"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKH81_09190"
     CDS             <1..308
                     /locus_tag="PKH81_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNQ98250.1"
                     /translation="IVSSFNPLALLAWKGIGGHTTAAIYCPELSVPFYMRHKECLYLS
                     GADIKKPSLETALASPGFETGKKPVLVWTVDTPSTAKLLLEGGVSAIITNRIQDWQS"
     gene            complement(305..880)
                     /locus_tag="PKH81_09195"
     CDS             complement(305..880)
                     /locus_tag="PKH81_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ98251.1"
                     /translation="MFSALEVANLCGVVNQTAINWIRNGHLKAFNTPGGQYRIYKDDL
                     LAFIKTRGMRVPEILQQGEPSANWNSLIVIDDDEALNNAIHSYFAKHLPDVTVYQSFD
                     GFDAGAQLVEKKPGFVILDLALPGVVGQEICKRIKTDPAFGRPFIIVITALSDDGLEA
                     EMLAMGADCFFRKPLTLSDILSSVTKAIEEN"
BASE COUNT          298 a          278 c          265 g          308 t
ORIGIN      
        1 atatagtctc gtcttttaat ccccttgccc tgctcgcctg gaagggaata gggggacata
       61 ccactgcggc aatttattgt cctgaactga gtgttccatt ttacatgcgc cacaaagaat
      121 gtctgtatct gtccggcgcc gacataaaaa aaccttctct ggaaacagcc cttgcttcgc
      181 caggttttga gacggggaaa aaaccggtgc tggtgtggac ggtagatacc ccgtcaacgg
      241 caaaactcct cctggaagga ggagtttcgg cgattattac caatagaata caggattggc
      301 agtcttagtt ttcttctatg gccttggtta cgcttgaaag aatatcggag agggtaagcg
      361 gtttccgaaa gaaacaatct gctcccatag caagcatttc cgcttccaga ccgtcgtcgg
      421 aaagcgcggt aataacgatg ataaacgggc ggccaaaggc tggatcagtc ttgatgcgtt
      481 tacaaatctc ctgaccaacg actcccggaa gggcaaggtc aagaatgacg aatccgggct
      541 ttttttccac aagttgcgcc ccggcatcaa aaccatcaaa ggattgatag accgtaacat
      601 ccggtaaatg tttggcaaaa taggaatgaa tggcgttatt gagggcctcg tcgtcatcga
      661 taacgataag actattccaa ttcgcgctgg gttctccctg ttgaagaatc tccggaacgc
      721 gcattcctcg tgtcttgata aaggccaaca gatcgtcttt ataaatacga tattgaccac
      781 caggggtatt aaaggccttg agatgaccgt tgcgtatcca gttaatggct gtctgattca
      841 cgactccaca gagattagcg acttcaaggg ccgaaaaaat ctgaattcgt tttgttttca
      901 attccttgct catactacct cggtaaaaaa ctatagccca taattggggc gctcacaagt
      961 aaggtttccc gttttttatt tttttttaag gcattcgcgg atagtccggg agctaaagcc
     1021 ctttcgtata agggcatcta gcaacttttt tccctctttt ccccgcttta caagcttctc
     1081 ccgtgctata cagcactgtt caagctcgga taccgttaat ccgaacgccg ccacggcgct
     1141 ttcggcaat
//
LOCUS       DAOQXQ010000634         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_167136_length_1146_cov_3.063245, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000634 DAOQXQ010000000
VERSION     DAOQXQ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167136_length_1146_cov_3.063245"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /locus_tag="PKH81_09200"
     CDS             <1..>1146
                     /locus_tag="PKH81_09200"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98252.1"
                     /translation="ADDPITDRVVAIIKEAIKRAGKRHDKNSLPLLQMHLARNEWLRS
                     RYKSAIRHFHKGRNLAETIDNPRIKNSTAIFSQFFLYWLGRYRDVVKSYEAFVPDIED
                     FPKENLPLLATLTVGACFAYTGQLSQGLGMLDAIRAKGRSIGNLSVTAVANVAIGQLL
                     METNRVDESRQYIEQALKEAKESRNLYAHIACLAFLALIHYHSDEHDKTVSTLREAIE
                     LGNRSQMPMRHGSVVIELCWAMEQGKLPYTAGLSLNEEINSALEGGNIFMKGVALRYR
                     ALNQHFKGLPDKDVIASLHRSIRYLNESGHTLEKAKAQLEMARAYLRLGRIEKAKQWA
                     TPAAQVLRGYNDSLIPDDLRPLVSVYSSNLNLLDEILYLGQELVTIR"
BASE COUNT          299 a          302 c          279 g          266 t
ORIGIN      
        1 cggcggatga tcccattacc gaccgcgtcg tcgccattat caaagaagca ataaaacgcg
       61 ccggcaaacg ccatgataaa aactcgctgc ccctgcttca gatgcacctg gcgcgtaatg
      121 aatggctcag gtcgcgctat aaatccgcca tcagacattt ccacaaaggg cgaaatcttg
      181 cagagaccat cgataatccc cggattaaaa attcaaccgc tattttcagt cagtttttcc
      241 tttactggct gggacgttac cgcgatgtcg tcaaaagcta tgaggctttt gttcccgata
      301 tagaagattt tccaaaagaa aacctgccac tgctggctac actgaccgtc ggggcctgtt
      361 tcgcgtatac aggacagttg tctcaggggt tgggtatgct cgatgcaatc agagcgaagg
      421 gccgcagtat cggcaatctc agtgtaacgg cggtggccaa tgtcgccatc ggacagttac
      481 ttatggaaac gaaccgggtt gacgaatcga gacaatacat tgaacaggct ctgaaagaag
      541 caaaggaaag ccgcaattta tacgctcata tcgcctgtct ggcttttctt gcccttattc
      601 actatcactc agacgaacac gacaaaaccg tttcgactct ccgtgaagca attgaactgg
      661 gcaaccgctc tcaaatgccg atgcggcacg gctctgtcgt gatcgaactg tgctgggcca
      721 tggagcaagg aaaacttcct tatacggctg gtctttccct gaatgaggaa atcaactccg
      781 ccctggaggg cggaaacatc ttcatgaaag gagtggcctt gcggtatcgg gcgcttaacc
      841 aacacttcaa gggacttccc gacaaagatg tgatcgcttc cttgcaccgt tccatccgct
      901 acctgaatga atccgggcat accctcgaaa aggccaaggc acaactggaa atggcgcgtg
      961 cctatctgcg gcttggacgt atcgaaaaag caaagcaatg ggcaacgcct gccgctcagg
     1021 ttctgcgtgg ttataacgat tcactgatcc ccgatgacct tcgtccttta gtgagcgttt
     1081 atagttcaaa tctaaacctc cttgatgaaa tcctctatct gggtcaggag ctggtaacca
     1141 tcaggg
//
LOCUS       DAOQXQ010000635         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_167852_length_1143_cov_3.453125, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000635 DAOQXQ010000000
VERSION     DAOQXQ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167852_length_1143_cov_3.453125"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PKH81_09205"
     CDS             <1..828
                     /locus_tag="PKH81_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNQ98253.1"
                     /translation="VIVVSILLQQYFAERIGSGRVEVLTLVGPGQSPHSYEPTPRQMA
                     LLSRSGVWVLSGTDFERTLTPKVQSQYPELTIIDGTAGAVFRSVEAHQHEEEGDGSHH
                     ESDTSALNLDRHTWLGRHNAKILAAHVRDALVLLDREGSSVYEKAYQETIADIDATFD
                     SLSRELMPMRGKTVFVFHPAFGYFLDEFGMSQEAVETGGKEPTPKTLSALITKARSEG
                     ITVIFVQKQFPVSAARELATSVGATVVPLDALASNWLENIRYMGASIAQSLASPARP"
     gene            complement(868..>1143)
                     /locus_tag="PKH81_09210"
     CDS             complement(868..>1143)
                     /locus_tag="PKH81_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNQ98254.1"
                     /translation="VAALTFGPKKTVVVAGYNKIVRDMREAEDRLELVASPLNNKRLG
                     TGNPCTKTGRCADCHADTRICKVYSVFKRKPGKSDFTVIIIGEALGY"
BASE COUNT          260 a          299 c          295 g          289 t
ORIGIN      
        1 gtaatagtgg taagtatcct gctgcaacag tactttgccg aacgcattgg ttcaggccgt
       61 gtggaggtgc ttaccctggt tggtcccggg caaagtcccc attcgtatga accaactccg
      121 cggcaaatgg ctcttctttc ccgctccggt gtgtgggttc tttcgggtac cgattttgaa
      181 cgtaccctta cccccaaggt tcaatctcaa tatcctgagt tgacgattat tgatggaaca
      241 gccggagctg tgttcaggtc ggtggaagca catcaacatg aggaggaggg ggatggctca
      301 catcacgaaa gcgacacttc cgctcttaac cttgaccggc atacctggct tggcagacac
      361 aacgcgaaga ttcttgccgc ccatgttcga gacgccctgg tgctgctgga tcgggaaggt
      421 tcatcggtgt atgaaaaggc ctatcaggaa acgatagccg atatagatgc aacctttgat
      481 tctctttccc gtgaactcat gccaatgcgg ggaaagacgg tctttgtctt ccatccggcc
      541 tttggctatt ttttggatga atttggcatg agccaggaag cggtagaaac cggaggaaag
      601 gagccaacgc ccaagaccct ttcggccctg ataaccaagg cccgttccga gggaattacg
      661 gttatttttg tgcaaaagca atttcccgta tctgcagccc gcgagcttgc tacctcagta
      721 ggcgcaaccg ttgtgccgct tgatgcgctt gcttccaact ggctcgaaaa catccgctac
      781 atgggcgcct ctattgccca gtcgctcgct tccccagcac ggccttaaga tacacggacg
      841 taagatacac ggccggattc ttccggccta gtagccaagg gcttcgccaa taataataac
      901 ggtaaagtcg ctttttcctg gttttcgttt aaaaacagaa tacaccttgc agatacgggt
      961 gtcagcgtgg caatctgcgc aacgccctgt tttagtgcag gggttccccg tgccaaggcg
     1021 tttgttgtta aggggagagg caacaagttc aagtcgatcc tcggcttctc tcatatcccg
     1081 aacgatttta ttgtatccgg caacaactac cgttttttta ggtccaaagg taagcgctgc
     1141 aac
//
LOCUS       DAOQXQ010000636         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_167916_length_1143_cov_2.480699, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000636 DAOQXQ010000000
VERSION     DAOQXQ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167916_length_1143_cov_2.480699"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1143)
                     /locus_tag="PKH81_09215"
     CDS             complement(<1..>1143)
                     /locus_tag="PKH81_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ98255.1"
                     /translation="DEETVFIPIEQVNLVQRYIGSEGEAPRLDRLGSKSWENRKNKVK
                     KSVEDIAARLIDLYSKRKATSGFPFPKDGEWQTAFEAAFPYEETEDQLTVSAEIKLDM
                     EKPSPMDRLVCGDVGYGKTEVAMRAAFKAVMGGKQVAFLAPTTLLAEQHYDSCISRFE
                     KFPVRIAQMSRFVTKADQKKTLERLARGEVDILVGTHRIIQKDILFKDLGLMIIDEEQ
                     RFGVKDKERLKELRTNVDSLALSATPIPRTLHMSLLKIRDMSLLATPPQNRHPIETFI
                     DEMNADRVAGAIRREMERGGQVFYLHNRVESLEETRIMLEKLVPEALVDIAHGQMSAG
                     ELDDIFRRFKMGGFNVLIATTIIENGIDIPNVNTIIIDRADMYGVS"
BASE COUNT          237 a          297 c          301 g          308 t
ORIGIN      
        1 tgggatacgc catacatgtc ggcgcggtcg ataataatgg tgttgacgtt gggaatatcg
       61 atgccgtttt ctataatggt agtggcaata agcacgttaa agccgcccat cttgaagcgg
      121 cgaaagatgt cgtccagttc gcccgcgctc atctgtccgt gggctatatc caccagggcc
      181 tcgggaacca gtttttccag catgatccgg gtttcctcaa ggctttctac ccggttatga
      241 agataaaata cctgtcctcc cctctccatc tcgcgtctga tggccccggc aacacggtcc
      301 gcgttcatct cgtcgataaa ggtttctatg gggtgacggt tttgtggcgg cgttgccaag
      361 aggctcatgt cgcgaatctt taaaaggctc atgtgtaagg ttcgtggaat gggggttgcg
      421 cttaaggcaa gagagtccac attagtcctc agttccttga gccgctcctt gtcctttacg
      481 ccaaagcgct gttcttcgtc gattatcatc aggccaagat ctttaaacag gatgtccttt
      541 tgaataatac ggtgtgtgcc caccaggatg tccacctctc cgcgcgcaag gcgctctagg
      601 gtcttttttt gatccgcttt agtaacgaag cgcgacatct gcgcgatgcg tacgggaaat
      661 ttttcaaagc gggagatgca gctgtcgtag tgttgttcgg caagaagggt tgttggcgca
      721 aggaaggcga cttgcttgcc gcccataacc gccttaaaag ccgcgcgcat agcaacctct
      781 gttttgccat agccgacgtc gccgcagaca agccggtcca tgggagaagg cttttccata
      841 tccagtttta tctcggcgga gacggtaagc tggtcttcgg tttcctcgta gggaaaggca
      901 gcttcaaaag cagtttgcca ttctccgtcc ttgggaaaag ggaaaccaga tgtcgcctta
      961 cgtttagagt acaagtcgat aaggcgcgcc gctatgtctt cgacggactt tttcacctta
     1021 ttctttctgt tttcccaact ctttgatccc aggcggtcaa gtcgcggcgc ttcgccctcg
     1081 ctgccaatgt aacgctgtac caggtttacc tgttcgatgg ggataaacac ggtctcttca
     1141 tcc
//
LOCUS       DAOQXQ010000637         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_167931_length_1143_cov_2.354779, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000637 DAOQXQ010000000
VERSION     DAOQXQ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167931_length_1143_cov_2.354779"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..617
                     /locus_tag="PKH81_09220"
     CDS             27..617
                     /locus_tag="PKH81_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98256.1"
                     /translation="MAFLYTAITIAPVHSYTVQFKEQFYKLYHVHYNQYPDDVMENIY
                     WLERALEADFANPLYAMGKIATPKDWEKYRYLFMMHINLKLTEQHLRLGSKWDKQRAY
                     FYNAPWKAQNLDSLHTAKTCYNTALVYWNEAKQWAEKANIREFRFLFLTDLQAWEDER
                     EGIANGRYNYGKTIERNLARLERVYNDFLAMDENTY"
     gene            627..>1143
                     /locus_tag="PKH81_09225"
     CDS             627..>1143
                     /locus_tag="PKH81_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNQ98257.1"
                     /translation="MDTFTFTPTTGPVSVHFYQELTVPTIPVSGGLYVVDTNTAPLLR
                     AAKGYAEHAPVIVIEAGESHKTLLNIEKILTAAFDAGLARDSLFVGFGGGVITDMTAF
                     AASLFMRGARVELVPTTLLAMADAAIGGKTGIDFKGLKNSVGTFYPAQAIHISVSALR
                     TLDRREFLTGLA"
BASE COUNT          328 a          259 c          247 g          309 t
ORIGIN      
        1 ggatacacca gtgggcggct ctaatcatgg cttttctata taccgccatt actatagcgc
       61 cagtacattc ctatacagtc cagttcaagg aacaattcta taaactctac catgtccatt
      121 ataaccaata tcccgacgat gtaatggaaa acatttattg gctagaacgg gcattagaag
      181 ccgattttgc aaatccgctg tatgcaatgg gaaaaatcgc gactccaaag gattgggaaa
      241 agtatcgcta tctttttatg atgcatataa atctaaaact gacggaacaa caccttagac
      301 ttggctcaaa atgggacaaa cagagagcgt acttttataa cgctccctgg aaagctcaaa
      361 acctggatag tctgcacact gctaaaactt gttacaatac cgctcttgtc tattggaatg
      421 aagccaaaca atgggcagaa aaagcgaata tacgtgagtt tcgtttttta tttcttaccg
      481 atcttcaggc atgggaagac gagcgagaag gaattgcgaa tggaaggtat aactacggaa
      541 agacaataga gcgaaacctg gctcgcctgg agcgagtcta taatgacttc cttgcaatgg
      601 atgaaaatac ttactaatat gtccccatgg acacttttac ttttaccccg actaccggac
      661 cggtttcggt acatttttat caagaactaa ccgttcccac tatcccggta tctggcggat
      721 tgtatgtagt tgataccaat accgccccgc tacttcgagc tgccaaggga tatgctgagc
      781 atgcacccgt tattgtaatc gaagcagggg aatctcataa aacccttctt aacatagaaa
      841 aaatactgac cgctgccttt gatgcaggtc ttgcccggga tagcttgttt gttggatttg
      901 gcggtggggt cataactgat atgacagcat ttgctgcatc cctttttatg cgcggagcta
      961 gagttgaact tgttcccact accctccttg ctatggccga tgccgcaatc ggcggaaaaa
     1021 caggtattga ctttaaaggc ttaaaaaata gcgtcggaac cttttaccct gcccaggcca
     1081 ttcacatttc ggtttctgca ttacgaaccc tggataggcg tgaatttctg accggtttgg
     1141 cag
//
LOCUS       DAOQXQ010000638         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_168723_length_1140_cov_2.427650, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000638 DAOQXQ010000000
VERSION     DAOQXQ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168723_length_1140_cov_2.427650"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKH81_09230"
     CDS             <1..411
                     /locus_tag="PKH81_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ98258.1"
                     /translation="GRHRGVSRYTIGQRRGIAIALGYPAYVIDKNAHDNTVTLGESAY
                     LFASALFARDVSFIPFEHLEGPLRCTARIRYNQGEQPATIYPCESDMVRVVFDEPQRA
                     IASGQSVVFFDGDLVVGGGIIAGIDRSSLTRPTP"
     gene            complement(392..>1140)
                     /locus_tag="PKH81_09235"
     CDS             complement(392..>1140)
                     /locus_tag="PKH81_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ98259.1"
                     /translation="GEVQKSLTGYGDLVEKLTSESMEGSRRVDGVFEETKGLKEMIGR
                     IEAELTTFSQRLSQVREFAAVIEQLAENTNVLAINASIQAARAGTAGKAFAVVAGEVR
                     TLAENSRHTAEDIRDMVGEITSNMAKILKESESGSNHVAQTLERAQEAKQSFESISSV
                     IRSANTEISRIEGAMSGILNMGSSVKGNMDEIDRMSNQSVDRLDEISRSVDELALQGS
                     HLSETANTLRTMATNQGIVFSQLSVKESDE"
BASE COUNT          254 a          316 c          285 g          285 t
ORIGIN      
        1 ggacgtcacc ggggcgtaag ccgctacacc attggtcagc gtcgcggcat tgcaattgcc
       61 cttggatacc cggcctatgt gattgataaa aacgcgcacg ataacaccgt taccctgggc
      121 gaaagcgcgt atctatttgc cagcgccctg tttgcccgtg atgtatcttt tattcccttt
      181 gaacacctgg aagggccact tagatgcacc gcccgaatcc gctataacca gggagaacaa
      241 ccggctacca tatacccctg cgaaagcgat atggtacgag tcgtctttga cgagccccaa
      301 cgagcgatcg cgagcggtca atcggtggtg ttctttgatg gagatcttgt ggtgggtggg
      361 ggaatcattg ccggaataga tcggtcctcg cttactcgtc cgactccttg accgagagtt
      421 gagaaaacac gattccctga ttggtggcca tggtcctaag cgtattcgcg gtttcggata
      481 gatgacttcc ctgcagggca agctcatcca cagagcggga tatttcgtcc aagcggtcaa
      541 cgctctgatt gctcatgcgg tcaatctcgt ccatgtttcc ttttaccgaa gagcccatgt
      601 ttaagatgcc actcatggca ccctctatac gggatatctc ggtatttgcc gatcgaataa
      661 ccgaactgat ggactcaaag gattgcttcg cttcctgtgc gcgctcaagt gtctgcgcaa
      721 cgtgattact gccgctttca ctttctttaa gaatctttgc catgttcgag gttatctcgc
      781 caaccatgtc gcgtatatcc tctgcggtat gacgcgagtt ctcggcaagg gtacgaacct
      841 ccccggcaac aacagcaaag gccttaccgg cggttccagc acgagcggcc tgaatggacg
      901 cgttaatcgc aagaacattg gtattctccg caagctgttc gattaccgca gcaaactcgc
      961 gtacctgtga aagacgctgg ctaaaggtgg ttaactcagc ctcgatgcgt ccaatcattt
     1021 ccttaaggcc cttggtctcc tcgaatactc catccacacg gcgagagcct tccattgatt
     1081 ctgaggtgag cttttctaca aggtcgccat aaccggtcaa ggacttttga acctcgccaa
//
LOCUS       DAOQXQ010000639         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_169686_length_1136_cov_2.629047, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000639 DAOQXQ010000000
VERSION     DAOQXQ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169686_length_1136_cov_2.629047"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PKH81_09240"
     CDS             complement(<1..955)
                     /locus_tag="PKH81_09240"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNQ98260.1"
                     /translation="MLAKRFYELSPYVPGEQPKDREYIKLNANENPYPPSPEVAKVIS
                     SFDVSSFRLYPDPDAGELRDAIAHMIGHGLTRDNLFVGNGSDEVLSFVFFTFFDSCAP
                     LYFPEHTYSFYPVYAGYYDIPIERKPLRADYSIDLDAFLAGSSTGVIFPNPNAPTGIY
                     TPVQTIRSFLQKYPADRAVIVDEAYIDFGGETVVSLLSDFPNLIIVRTFSKSFCFAGA
                     RLGFVIANPPVIQNLFTTKNSFNHFPVDTLTQKIGTAACGDWPYYRRINDEIARTRDM
                     FAQEITAYGWTVLPSLANFVFTRKEGLSGKSIYEAIKREGIL"
BASE COUNT          345 a          240 c          245 g          306 t
ORIGIN      
        1 ccaaaattcc ttccctttta atagcttcgt atatgctctt gccagatagt ccttctttgc
       61 gagtaaaaac aaaattagct aacgaaggta aaacagtcca tccataagcg gttatctctt
      121 gggcaaacat atcacgggtt cgtgctatct catcgttaat tctgcggtaa tacggccaat
      181 caccacaggc tgccgttcca atcttttggg ttagggtgtc tacaggaaag tgattaaagg
      241 agttctttgt ggtgaacagg ttttgtatta cgggaggatt ggcaataacg aacccaagcc
      301 gtgcccccgc gaaacaaaaa cttttggaaa aggtgcgaac aataattaaa ttaggaaaat
      361 cggacaagag agacaccact gtttctccgc caaaatcgat atatgcttca tcaactatta
      421 ccgctcgatc agcgggatac ttttgtaaga aggagcgaat cgtttgaaca ggggtataga
      481 tgcctgtggg tgcgtttgga ttgggaaaga taacccctgt ggacgatcct gcaaggaaag
      541 catccaaatc aattgagtaa tctgctcgca acggttttct ttctatcgga atgtcataat
      601 aacctgcata taccggataa aagctgtatg tgtgttcagg aaaataaaga ggggcgcaag
      661 aatcgaagaa agtaaaaaag acaaacgaga gaacttcatc cgatccgttt ccaacaaaaa
      721 gattgtcacg cgtaagtcca tggccaatca tgtgtgcaat cgcatctctc aactctcccg
      781 catctggatc gggatacaat cgaaacgatg aaacatcaaa cgatgaaatt actttggcta
      841 cctctggcga aggaggatac ggattttcgt tcgcgttgag ttttatatat tcgcgatcct
      901 ttggttgttc tccgggtaca tacggagaca gttcataaaa acgttttgcg agcatcttat
      961 ctctccacca ctgagcgtat cattgtatga aaatcgtaca actttttttt aaggttgacc
     1021 agtatgatga gctgacatat actcaagagc atatccaccc actcaggagt ttatccaaat
     1081 gaaaacagta tctttttcct tcgtcgcgaa gtatccggcc aaaaaagtag ccgact
//
LOCUS       DAOQXQ010000640         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_171198_length_1130_cov_2.576744, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000640 DAOQXQ010000000
VERSION     DAOQXQ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171198_length_1130_cov_2.576744"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKH81_09245"
     CDS             complement(<1..601)
                     /locus_tag="PKH81_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNQ98261.1"
                     /translation="MQEPRILITQELLKLIAEIDEFKGKWEVLKNLSPERLRQLRKVA
                     TIESIGSSTRIEGAKLTDMQVETLLSNLSSMSFKTRDEQEVAGYAEAMDIVFQAYEDM
                     TITENHIRQLHQTLLRHSDKDERHRGEYKKIDNHVVAIDEHGKEIGVVFETATPFDTP
                     RKMEELVRWVNKAITENSFHPLLIVAVFVVVFLAIHPFQD"
     gene            806..>1130
                     /locus_tag="PKH81_09250"
     CDS             806..>1130
                     /locus_tag="PKH81_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98262.1"
                     /translation="MSKYERLSYKVSARIARKNNAVFVRKDFEDLGGYDQIGRILRQL
                     AAAGKLVRIGYGLYAKAKRSALTGEVIPILPLPTLAKEALERLGIEIGASQLEKNYNA
                     GITTQV"
BASE COUNT          297 a          259 c          250 g          324 t
ORIGIN      
        1 catcctgaaa cgggtggatg gccagaaaca ccaccacaaa gaccgccacg atcaatagcg
       61 ggtgaaagct gttctccgta atcgctttgt tgacccatcg caccagttct tccattttgc
      121 gcggggtatc gaagggtgtg gccgtttcaa aaaccacgcc aatttccttg ccgtgctcat
      181 ctatggcaac aacgtgatta tcgattttct tgtattcccc ccggtggcgt tcatctttat
      241 cgctgtgccg caagagcgtt tgatgaagtt gtcggatatg gttttcggta atggtcatat
      301 cctcgtaagc ttgaaagacg atatccatcg cctcggcata accggccact tcctgctcgt
      361 cacgggtctt gaaggacatg gacgatagat tggacaacag ggtttccacc tgcatatctg
      421 tcagctttgc tccctcgatc cgagtagacg aaccgatact ttcaatggtt gccaccttgc
      481 gaagctggcg cagccgctcc ggggatagat ttttcaaaac ttcccacttg cctttaaatt
      541 catcaatctc ggcaatgagc tttaataatt cttgagtaat gagaattctg ggttcttgca
      601 tgaaagtttt ctatatccga ttctatatcc gattctgtac ccgatcatac ccgattacac
      661 ccgatctaac cggttaaaac atatttatca aacagctgaa aactaaggtt cctcaccctg
      721 attaagcttg aattttgata cataatgtcg tatgtttgta acaggtatat gtagtgtttt
      781 tgtatatata aatggctgac ataaaatgtc taaatacgaa agattatcat ataaagtatc
      841 tgcaagaata gcgaggaaaa ataatgctgt ttttgtccgt aaagattttg aagatttagg
      901 cggatatgac cagattggcc gtatactgcg ccagcttgcg gctgccggaa aactcgtcag
      961 aattggatat ggcctctatg ccaaggctaa acgttctgcc ctgacgggag aggttatacc
     1021 cattcttcct ttgccaacct tggctaaaga agccttggag cgtctgggca ttgagatcgg
     1081 cgcttcgcaa ctggaaaaaa attataatgc gggcataacg actcaggttc
//
LOCUS       DAOQXQ010000641         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_172185_length_1126_cov_2.616246, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000641 DAOQXQ010000000
VERSION     DAOQXQ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172185_length_1126_cov_2.616246"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1126)
                     /locus_tag="PKH81_09255"
                     /pseudo
     CDS             complement(212..1126)
                     /locus_tag="PKH81_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          231 a          274 c          265 g          356 t
ORIGIN      
        1 gtggtaaaat gcgaccttcc caaggtcatg tcgcgggtcc gattcccgtc gcccgctttc
       61 tgataatgca tgcatcttaa ccttaccgat gaagtcggta aggttttttt atttaatgcc
      121 tgctgaataa atccctattt ttagcttacg gcttcattag aggttcaatt ctaggggagc
      181 tcatccattg gatgaaagtc aaaaaaatgc gcaatgaaac cttggacagg ttcattgtaa
      241 ggatggataa agcaatcaca cagagactgg tctcttcaag gcgtgttcga ataagcctga
      301 gcccgaactt tcttttcgcc aggctgaagc ctctctcgac ctctactcga tcgcagttgt
      361 cgctgtattc ttgttttttg tcaatcttct gatccttctt gggcctgccg agcgacgggc
      421 cggataatcg gattccgagt tctttgcagt agcttaagtt tgtgcgattt cggtatattt
      481 tatccgccaa aactcgttca ggatacactc caatccgttc tttgtagcgt tccaccgcaa
      541 ctatgaggca ttcgctctcg ttgtaggcat cgaaggaaat tttttcgagt cgggcgtacc
      601 cattggttat gctcatgtcc agtttggctc cgaactccac cgggttcttg gcttttccgc
      661 ggacaatcgg gcggataaac ggctggctga tactgacgat gcgatcctgc actttgtgcg
      721 tcctgcttgt gtgcatgtag agctgctgtt catacaattt ccgcaaagtc cccaacaggt
      781 cggcttgctt cgttcctagc gtataacctt gctccagaaa tgcgtcgatg taacctaaat
      841 cccgccggat gtagttcagt tgcttgccga tggctttgcg aatttgtttg ccacttttct
      901 ttttcttgcg tgcgatgttg agataatccc ttcgggctat tttccgatag gtacgcggtt
      961 tgggaaggtg gtttgcttcg cagatttcgt cgatgatgcc ttccaacttt tcccgacatt
     1021 cgttcaacag ttccgtgtct tgcgggaact tgatttctga aggcgcgcaa gtggcatcta
     1081 gaattagcgt cccttcattc ttagatcccg atgcatcatt gtcatc
//
LOCUS       DAOQXQ010000642         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_172969_length_1123_cov_2.470037, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000642 DAOQXQ010000000
VERSION     DAOQXQ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172969_length_1123_cov_2.470037"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..831)
                     /gene="truA"
                     /locus_tag="PKH81_09260"
     CDS             complement(37..831)
                     /gene="truA"
                     /locus_tag="PKH81_09260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNQ98263.1"
                     /translation="MAMKRKNESEALRNILLIISYDGSRFHGWQAQPVRGCEEDRTVQ
                     GVIESALAKMHGHRVPLIGSGRTDSGVHAVGQAANFYTDIASIPEDRFVPALNSLLPR
                     DVRILSSRSVHPDFNARFSARGRTYRYFLNCALTPFAHEIPYSWHIGRRPDLVTLNRM
                     AAYLCGEIDFTTFTAAGDQSPSRCRYMYGASFFPEGNKVVFEISANAFLWKMVRSLTG
                     TLLHLEKSGAAPEVFKQILDERNRSLAGPTAPAEGLFLWHVRYDSL"
     gene            complement(809..>1123)
                     /locus_tag="PKH81_09265"
     CDS             complement(809..>1123)
                     /locus_tag="PKH81_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNQ98264.1"
                     /translation="RAIELETTGKEMISNLKSFGPDLDKNYGYDGVLYLTALLEYKYG
                     SRTNPEKRVELLSYQRRSLAKMFGLGRSSKNKPGPLLEHARNLYDNIVVELNDNGDEE
                     EE"
BASE COUNT          295 a          288 c          268 g          272 t
ORIGIN      
        1 cccccagagc ctgctgtagc aggaaaagaa acacccttaa agactatcgt agcgtacatg
       61 ccacaaaaaa aggccttccg ccggcgccgt cggacctgcc agagagcggt ttctctcatc
      121 aagaatttgt ttgaacactt cgggagcggc tccgcttttt tcaagatgca acagggtccc
      181 ggtgagcgat ctgaccattt tccataaaaa agcattggca gaaatctcaa aaacgacctt
      241 gttcccttcg gggaaaaaac tggccccgta catataccga cagcgacttg gactttgatc
      301 gcctgctgcg gtaaaggtgg taaagtcaat ttctccacac aggtatgcgg ccatacggtt
      361 aagcgttaca agatcgggcc tgcggccgat atgccaggaa tagggtatct cgtgggcaaa
      421 gggagtgagg gcacaattga gaaagtaccg ataggtcctt ccccgtgcac taaagcgagc
      481 attaaaatct gggtgtactg atcgagacga gagtattcga acgtcccgag gcaacaaaga
      541 gttgagcgcg ggaacaaaac ggtcttccgg tatggaggcg atatcggtat aaaaattcgc
      601 ggcctgcccg actgcatgaa cacctgaatc agttctgcct gagccaatta agggaaccct
      661 atgtccgtgc atcttggcca gggccgactc aataacaccc tgtaccgtac gatcttcttc
      721 gcatccacgc accggttggg cttgccaacc gtgaaaccgg cttccgtcgt acgagattat
      781 tagcaggatg tttcgcaaag cttccgattc attcttcctc ttcatcgcca ttgtcattaa
      841 gttccactac tatgttgtca tacagattgc gggcatgttc caaaagcggg cccggcttgt
      901 tttttgaaga tcttcccaaa ccgaacatct tggcaagaga gcgcctctga taggataata
      961 attcaactct tttttccgga tttgtccggg atccatactt atactcaagc aatgcggtaa
     1021 gatacaacac accgtcatag ccatagttct tatcaagatc ggggccaaag cttttaaggt
     1081 tagagatcat ttccttacca gtggtttcca actctatcgc gcg
//
LOCUS       DAOQXQ010000643         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_174024_length_1119_cov_3.090226, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000643 DAOQXQ010000000
VERSION     DAOQXQ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174024_length_1119_cov_3.090226"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="PKH81_09270"
     CDS             complement(<1..91)
                     /locus_tag="PKH81_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNQ98265.1"
                     /translation="MKFHVLTLFPEIPRAFFESSIMAKAVSRGI"
     gene            complement(100..669)
                     /gene="rimM"
                     /locus_tag="PKH81_09275"
     CDS             complement(100..669)
                     /gene="rimM"
                     /locus_tag="PKH81_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685696.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNQ98266.1"
                     /translation="MDERGVTREADNLLITGIIRTSHGLDGFVKVESSSGEVDHFADL
                     SEIFARQPGSNKEEKLQRLFIEAVEGSSSLLLIKFKGIDSPEQAKKLAGFELLVPRDK
                     ACPLNEGEYYVGDLCQCALVYNGCPIGTIIGVMEGGADDLLEVILTDGSDLDESRPIK
                     RLVPLRKEFVGKIDISGRTVELMHRWILE"
     gene            complement(669..902)
                     /locus_tag="PKH81_09280"
     CDS             complement(669..902)
                     /locus_tag="PKH81_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNQ98267.1"
                     /translation="MEKDLVEYIAKSLVDDPGAVTVSEVEGEKSTILELRVAPADIGK
                     VIGKYGRIAKAMRTVLSASSGKSGKRVSLEILD"
     gene            complement(927..1118)
                     /gene="rpsP"
                     /locus_tag="PKH81_09285"
     CDS             complement(927..1118)
                     /gene="rpsP"
                     /locus_tag="PKH81_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNQ98268.1"
                     /translation="MVVQDSREPRDGKTLDEVGIYHPIEAEEKQVSFDSDRVRVWIDR
                     GAQPTDTVRGLLNRKNFTL"
BASE COUNT          276 a          310 c          253 g          280 t
ORIGIN      
        1 taattccccg cgaaaccgcc ttggccataa tcgaactttc gaaaaaggcc cgaggtatct
       61 cggggaataa ggtcagcaca tggaacttca tgaagcgttt tactccagaa tccagcggtg
      121 catcagctct acggttcgtc ccgaaatgtc gatcttgccg acaaactcct tgcggagggg
      181 aacaagccgc tttatcggcc tggattcatc caggtcggag ccatcggtca ggataacctc
      241 gagaaggtca tccgcgccgc cttccattac accgataatt gtccctattg gacagccatt
      301 gtatacaaga gcacactgac aaagatcacc gacatagtac tccccctcat taagaggaca
      361 agccttgtct cgaggaacca aaagctcaaa gccggctaat ttcttggcct gctcgggact
      421 gtcaattccc ttgaatttaa tgagcaacaa ggatgaagaa ccttcaaccg cctctatgaa
      481 caggcgttgt agtttttctt ccttgttgct tcccggttgg cgggcaaaaa tctctgaaag
      541 gtcggcaaaa tgatctactt caccggaaga actttcaact tttacaaacc cgtccagccc
      601 gtgtgaagtg cgaataatcc ccgtaataag aagattatca gcctcacggg ttactccccg
      661 ctcatccatc aatcaaggat ttccaaagat actcttttac cggatttccc tgaagaggcg
      721 ctcagcaccg tacgcattgc cttggcgata cgtccgtatt tgccgattac cttgccgata
      781 tcagccggcg cgacacggag ttcgaggatg gtggactttt cgccctcaac ctcgctaacc
      841 gttaccgcac cgggatcgtc gaccagggat ttagcgatat attcaaccaa atctttttcc
      901 acttctcggt gccctcttcc cgtgtcttac agggtgaaat tcttgcgatt taacaatccg
      961 cgaacggtgt ctgtcggctg tgcgccgcga tcaatccata cgcgtacccg atctgagtca
     1021 aaagaaacct gtttttcttc agcttcgatg gggtggtaaa tacctacctc gtcaagggtt
     1081 tttccatcgc ggggttcgcg agaatcctgg acaacgatg
//
LOCUS       DAOQXQ010000644         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_174071_length_1119_cov_2.467105, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000644 DAOQXQ010000000
VERSION     DAOQXQ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174071_length_1119_cov_2.467105"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKH81_09290"
     CDS             complement(<1..391)
                     /locus_tag="PKH81_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ98269.1"
                     /translation="MEAFGKRLADARNARGIDFDQASRETNITRRYLEALEREDFTVF
                     PGEPYLLGFLRNYCDYLGLDSNEVITSYKNLKIQEAPIPLKDLMPRRPLTDRLGGSLF
                     LVRAALAVVVIALVGFLVWGGISLASSL"
     gene            complement(415..1113)
                     /locus_tag="PKH81_09295"
     CDS             complement(415..1113)
                     /locus_tag="PKH81_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNQ98270.1"
                     /translation="MITHANRILSLVVLSLLCAFSAFSQTITTADSFFSSVSEVYAGI
                     KDYSATITITSVSGNKSEEMVGRAAFKRPNLLRIDFSKPDEQTIVFNGEILTIYLPAY
                     NVVLSQTVEKKSGAAGASLATPEGLSLMKRYYTIAYATGPEPVQLDEKSREQVVVLLL
                     SRRSTTEMFRTIKLMINPSNKSIRRIEAETIAGDTVLFDFTGYAFNQGILDNRFVYDS
                     PASANMFNNFLFSE"
BASE COUNT          325 a          269 c          238 g          287 t
ORIGIN      
        1 aaagagaact ggcaagactg atgccacccc ataccaaaaa gcccacaagg gcaatgacta
       61 ctacagccag ggcggcgcga acaagaaaca aactgccacc cagtcgatct gttaaaggac
      121 gacgaggcat taaatcctta agcggaatag gggcttcttg aatcttaaga tttttatatg
      181 aagtaattac ctcgttactg tcgagtccca aatagtcgca gtaattgcga agaaatccca
      241 acaagtacgg ttcgccggga aatacggtaa aatcctcgcg ttccagggcc tcaagatatc
      301 tcctggtgat atttgtttct cgggatgcct ggtcaaaatc tattccccgg gcattcctgg
      361 catcggccag acgttttcca aaagcttcca taccttaaac cttctccact ttttttactc
      421 gctaaacaaa aaattattga acatattcgc cgatgcggga gaatcatata caaaccgatt
      481 atcaaggatt ccctggttaa acgcatatcc ggtaaaatca aaaagcacgg tgtctccggc
      541 gattgtctcg gcctctatcc gtctaatact cttgttgctt gggtttatca taagcttgat
      601 tgtcctgaac atctcggtag tacttcggcg agacaagagc agaaccacca cctgttccct
      661 cgatttttca tcaagctgca ccggttcggg ccctgttgca taggctattg tgtaatagcg
      721 cttcatcaag ctcaatcctt ccggtgtcgc aagcgatgcg ccagcagctc cggatttttt
      781 ttcaactgtt tgactcaaca ccacattata ggcaggtaaa taaatagtta aaatttctcc
      841 attaaataca atggtctgct catctggctt ggaaaaatca attctgagca ggttgggacg
      901 tttaaacgcg gcgcgaccaa ccatttcctc ggatttattt ccggaaacgc ttgttatggt
      961 aattgtagcc gaatagtcct tgataccagc atagacttcc gaaacagagg aaaaaaacga
     1021 atccgcggtg gttattgtct gggaaaaagc gctaaaggcg cacaagagcg acaacaccac
     1081 caaagaaagg atacggttag catgcgtaat catggtatc
//
LOCUS       DAOQXQ010000645         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_174550_length_1117_cov_2.928437, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000645 DAOQXQ010000000
VERSION     DAOQXQ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174550_length_1117_cov_2.928437"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="PKH81_09300"
     CDS             complement(<1..>1117)
                     /locus_tag="PKH81_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ98271.1"
                     /translation="GITRLQKKTLDAMVAYCQDHLADQPKMEYETAMSAISARSGSSI
                     YIKKIDDYAWREIDDESHLEMAVREVFPRILERESTRAVRREVLLNPGPATTTDSVKM
                     SQICADICPRESEFGDVMEWISEELSLFVGDKGRVETVLFGGSGTAADEVMISSCVPD
                     GAKLLIVDNGAYGARFAKIASVYKLDYEVLSSSGFLPLDIKAVQTRLVDGKFTHFAIV
                     YHETTTGLLNPVPQLCRFCHDKGIVTIVDAVSAFAAIPIDMDRDCIDFMASTSNKNIQ
                     GMAGVAFVFCRKEALDRVMDYPMRNFYLNLPDQYKYFKKTHQTRFTPPVQAMYSLRQA
                     IIETKIETIEGRYTRYSECWKILLDAVKSLGLTMLVP"
BASE COUNT          288 a          285 c          262 g          282 t
ORIGIN      
        1 tcaggaacga gcatggtaag gccaagtgac ttaacggcgt ccaagaggat tttccagcac
       61 tcactatagc gtgtataccg gccctcaatc gtctcgatct ttgtttcgat tatagcctgc
      121 ctcaggctat acatcgcctg aacaggagga gtaaagcgag tttgatgggt cttcttgaaa
      181 tacttgtatt gatcgggaag attaagatag aagttacgca tgggataatc cataacacga
      241 tccagggctt cctttcggca aaagacaaag gcgacacccg ccataccttg aatattcttg
      301 tttgaagtgg atgccataaa gtcgatgcaa tcccggtcca tatcgatagg aattgcagcg
      361 aaggcgctca ccgcgtccac gatggttacg atacccttgt catgacaaaa cctgcacagt
      421 tgtggcacgg gattaagaag ccctgtggtc gtctcgtgat acactatggc aaaatgggta
      481 aactttccgt caacaagtct ggtttgaact gccttaatat caaggggcag gaacccgctt
      541 gaggatagca cttcgtaatc aagtttatac actgaagcaa tcttggcaaa acgggctcca
      601 tacgcgccat tatcaacgat cagaagtttt gcgccgtcag gaacacacga ggatatcatt
      661 acctcatccg ctgccgttcc cgagccgcca aagagaactg tttcaacccg gcctttatct
      721 cccacaaaaa gagaaagctc ctcggatatc cattccatga cgtccccgaa ctcgctttct
      781 cgcgggcaaa tatcggcgca aatctggctc atcttgacgc tgtcggtcgt agttgccgga
      841 cctggattaa ggagaacttc cctgcgtacc gcccgagtgc tttctctttc caagatacgg
      901 ggaaagactt cccgtaccgc catctcaaga tgactttcat cgtcgatttc ccgccatgcg
      961 taatcgtcga tttttttaat ataaatggaa gatccggagc gcgcgcttat agcgctcatg
     1021 gcggtctcat actccatctt gggctgatcg gccagatgat cctggcaata tgcaaccatt
     1081 gcatcaaggg ttttcttttg aaggcgagtt atgccga
//
LOCUS       DAOQXQ010000646         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_175903_length_1112_cov_2.470199, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000646 DAOQXQ010000000
VERSION     DAOQXQ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175903_length_1112_cov_2.470199"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..>1112
                     /gene="cysK"
                     /locus_tag="PKH81_09305"
     CDS             226..>1112
                     /gene="cysK"
                     /locus_tag="PKH81_09305"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNQ98272.1"
                     /translation="MARVEKITDLIGNTPLVRINSLARDIQAEIYVKLESFNPAGCVK
                     ERIALSMIESGEKDGSITTGTTLIEPTSGNTGVGLAMVAATRGYRLILAMPETMSMER
                     RTLFKAYGAELVLTDGAKGMKGAIERAEDLSREIPGSYILQQFNNPANADIHRITTGP
                     EIWADTEGRVDILVSGVGTGGTITGTGEFLKEKKPTIQVVAVEPEGSAVLSGGKAGPH
                     KIQGIGAGFVPGVLNTSIIDEVYLTDEHKAGSIARRLAREEGILVGISSGAALEAALE
                     IGRRKENAGKVIVAILPDTG"
BASE COUNT          293 a          244 c          310 g          265 t
ORIGIN      
        1 cgcagcctgg cgaggcggaa actccttttc aacgatgtat acgtctaact gtctataatc
       61 ggcttaatga gcgaatagcc gctcttgtgg atcatacaac cttggcttcg ctgattgggt
      121 cgggggattc aacgaattat tcaatttaag aatattgact ttcatatcat cctgtagtat
      181 ataataccta taagtttact aggaattagt aaacggggag gcataatggc cagagtcgaa
      241 aaaatcacgg atttgatcgg aaacaccccg ctggtacgga ttaattccct tgccagggac
      301 attcaggcgg aaatctatgt aaagctggaa agctttaatc ccgccggatg tgttaaggaa
      361 cgcatcgccc tttcgatgat cgaatctggg gaaaaagatg gttccattac aacgggtaca
      421 acgcttatag agccgacgag cggtaatacc ggcgttggtt tggcaatggt cgccgcaacc
      481 aggggctacc gtcttattct tgcgatgccc gaaacgatga gtatggaacg gcgtaccctt
      541 tttaaggcct acggggccga gctcgtactt accgacggag ctaagggaat gaagggcgcc
      601 atcgagcgtg cagaagatct ttcccgcgag atccccggct catatatatt gcaacaattc
      661 aataatcccg caaacgcgga tattcatcga ataactaccg gcccggagat atgggcggat
      721 accgaaggca gggtggatat tctggtaagc ggtgtaggaa ctggtggaac aataactggc
      781 acgggagaat ttctaaaaga gaaaaaaccg acgatccagg tagttgctgt tgaaccggaa
      841 ggttctgctg ttttgtccgg gggtaaggcg ggaccgcata agatacaggg aattggggct
      901 ggctttgtgc ctggcgtcct taatacctct attatcgatg aggtctatct tactgacgaa
      961 cataaagcgg gttccattgc ccgtagactt gcccgggaag agggcatcct tgtgggaatc
     1021 tcgtcgggag ccgcgcttga ggcggccctg gagattggca gacggaaaga aaacgccggc
     1081 aaggttattg tcgcaattct tccggatact gg
//
LOCUS       DAOQXQ010000647         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_176148_length_1111_cov_2.749053, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000647 DAOQXQ010000000
VERSION     DAOQXQ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176148_length_1111_cov_2.749053"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKH81_09310"
     CDS             <1..215
                     /locus_tag="PKH81_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HNQ98273.1"
                     /translation="SRRYTISALVRGGIADESGFSENDVVEIRDIQFPKDKNIVTLQL
                     FTKRRSKGYLEAFIGLGASLDSPSYF"
     gene            228..>1111
                     /locus_tag="PKH81_09315"
     CDS             228..>1111
                     /locus_tag="PKH81_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ98274.1"
                     /translation="MTDTRFIRNFCIVAHIDHGKSTLADRLIEKARVIDERFYHAQMT
                     DNMDIERERGITIKSQAVTIPYVANDGNTYLLNFVDTPGHVDFTYEVSRAIASCEGAL
                     LIVDAAQGVESQTLSNMYLALEHNLEILPVINKIDLPAADIPSTLHQINHDLGLDPDY
                     ACQVSAKQGIGIDELFEAIVARIPPPVGRSDDLLRALIFDCHYDSYRGVVIHIRVFTG
                     RIRKGQTIRFMNSQTDYKVEDTGIFLLKLVPSEELAAGDVGYIIAGIKTVQDVRVGDT
                     ITNADNPCDKPLPGFKEV"
BASE COUNT          291 a          267 c          254 g          299 t
ORIGIN      
        1 caagccgcag atacaccata tctgccctgg ttagaggcgg tattgccgac gagtcaggtt
       61 tttccgaaaa cgatgtagtg gaaattcggg acatacagtt ccccaaggac aagaatatag
      121 taacccttca attgttcacc aaacgtcgca gcaagggcta tctggaagcc tttatcggcc
      181 ttggcgcatc tctggacagt ccctcctatt tctaatatta tacgcttatg acagacaccc
      241 gttttatccg caacttttgc atagtagccc acatcgatca tggtaaatcg actcttgcag
      301 atcggctgat cgaaaaagcc cgcgtaattg atgagcggtt ttatcatgcg caaatgaccg
      361 ataacatgga cattgaacgg gaacgcggta ttaccataaa gagccaggcg gtaacgattc
      421 cgtatgtggc aaacgatgga aacacctact tgcttaactt tgttgacacc ccgggtcatg
      481 ttgactttac ctacgaggtt tcccgagcca tagcgtcttg cgaaggcgcc ttgttgattg
      541 tagatgctgc ccagggagtt gaatcgcaaa ctctttccaa catgtattta gcccttgagc
      601 ataatcttga aattttgccg gtaataaaca agatagacct tcccgccgcg gatataccaa
      661 gcacattgca ccaaattaac catgaccttg gacttgaccc ggattatgcc tgccaggtat
      721 ctgccaagca aggcattggc atagatgaat tgtttgaggc aattgttgct cgtattcctc
      781 ctcccgtcgg acgttctgat gatctattgc gagccttaat ctttgattgt cattatgact
      841 cgtatcgtgg tgtcgtcatt catattcgcg tgtttaccgg tcgaatccgt aaaggacaaa
      901 ccatccgctt tatgaattcg cagacggatt ataaagtcga agacaccgga attttcctgt
      961 taaagctcgt tccctccgag gagcttgcag ccggtgatgt aggatatatt atcgcgggta
     1021 tcaagaccgt acaggatgtg cgcgttggcg atacgataac caatgcagat aatccctgcg
     1081 acaaaccctt gcctggcttt aaggaagtta a
//
LOCUS       DAOQXQ010000648         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_176960_length_1108_cov_2.886040, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000648 DAOQXQ010000000
VERSION     DAOQXQ010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176960_length_1108_cov_2.886040"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKH81_09320"
     CDS             complement(<1..912)
                     /locus_tag="PKH81_09320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ98275.1"
                     /translation="MWVPLQITTAPQVISNSSRENLLFGLFFGIIIAIFFVNLFSYLV
                     MSNKNFLIYMCYLFSLFLYHLRVHGFMYFVPMPFMVLESVLWISLAGVGIFMMIFAKH
                     FLNLSRTCPQINRILNVFIGLFVIQTIFGIFISSLIANYIAYITGFLVPLIILVTSIK
                     LYITGFKEARFYLLALFAMFTGTLIWSTAAYAEAHIPANYFFLIGTSLDSLLFTLAIF
                     DLMRKEIGEKDIMIEREKYYINLSRTDPLTGLYNRRYLNDLVKRLESEGELPAESAMI
                     MLDLDNFKIINDTHGHLVGDAILRQLAK"
     gene            911..1057
                     /locus_tag="PKH81_09325"
     CDS             911..1057
                     /locus_tag="PKH81_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98276.1"
                     /translation="MIESVFTRRYTGFVVSIVSGIGRFQILVGMAISRTSTFFPATNL
                     VPSG"
BASE COUNT          346 a          217 c          268 g          277 t
ORIGIN      
        1 cttggcaagt tgccgcagaa tcgcgtcgcc cacaagatgg ccgtgagtat cgttaatgat
       61 tttgaagtta tccaaatcga gcatgatcat ggctgattct gcgggtagct ccccttctga
      121 ttccagacgc tttacaaggt cgttaagata gcgtcggttg tataaaccgg taaggggatc
      181 ggtgcgagaa agatttatat agtatttttc tcgctcgatc atgatgtcct tttccccgat
      241 ttccttacgc atcaaatcaa aaatagccag ggtaaataga agtgaatcaa gcgaggttcc
      301 aatcaggaaa aaataattcg ccggaatatg agcctccgca tacgctgcgg tggaccaaat
      361 aagagttccg gtaaacatgg caaaaagggc gagtagataa aatcgtgctt ccttaaaacc
      421 ggtaatatag agtttgatgc ttgtaaccaa gatgatcagg ggaacaagaa agccggttat
      481 ataggcgatg taattggcga taagacttga aataaaaatg ccaaagatgg tttgaatgac
      541 aaacaaacct atgaatacat tcagaattcg atttatctgc gggcaggtcc tggagagatt
      601 caggaaatgc ttggcaaaga tcatcataaa aatgccgact ccggctaggg aaatccagag
      661 cactgattca agaaccataa acggcatggg cacaaagtac ataaacccgt gaacccgcag
      721 atgataaagg aagaggctaa agagataaca catgtaaata agaaaattct tgttgctcat
      781 gacgagatac gagaagaggt taacgaagaa gattgcgatg attatcccaa aaaacagccc
      841 aaaaagcagg ttttcgcggg aactgtttga gatgacctga ggcgcggttg taatttggag
      901 gggcacccac atgatcgaat cagtttttac ccgcaggtat accggctttg ttgtatccat
      961 tgtatcgggg ataggcaggt tccagattct ggttggtatg gcgatttccc gtacatctac
     1021 ctttttcccg gccactaatc tggttccatc agggtaaaaa acgtctatgt tctccagact
     1081 tgcgtttttg attgaaataa agcagcgc
//
LOCUS       DAOQXQ010000649         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_178407_length_1103_cov_3.132634, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000649 DAOQXQ010000000
VERSION     DAOQXQ010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178407_length_1103_cov_3.132634"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1103)
                     /locus_tag="PKH81_09330"
     CDS             complement(<1..>1103)
                     /locus_tag="PKH81_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ98277.1"
                     /translation="RRKDGSPSKGTVLVLGSGPIRIGQGIEFDYCTVHCVWALRELGY
                     ETIIVNNNPETVSTDFDISDRLYFEPLTAEDVADIVDNEKPVGAVVQFGGQTAIKLAK
                     AVAAMGVPILGTSARDVDRAEDRELFDQVLEETAIPRAAGKTVFTAEQAIDASHELGY
                     PVLVRPSYVLGGQGMEIAYSDDDIVEFMEIINRSEQENPILVDKYIEGCEAEVDAVCD
                     GENILIPGIMEHVERTGIHSGDSISVYPPIHLTITQKEKMANYAAKLAKSLKVIGLIN
                     IQFIAKGDDIYVIEVNPRSSRTVPYISKVTGLPIVDIATRCMFGEKLADMEWGVGLYR
                     EASYYAVKMPVFSFEKLHDVEIALGPEMKSTGE"
BASE COUNT          266 a          298 c          241 g          298 t
ORIGIN      
        1 ctctccggtt gatttcattt ccggaccaag ggcaatttcc acatcatgta gtttttcaaa
       61 gctaaacacc ggcatcttta ctgcgtagta cgaagcctcg cgatacaaac caactcccca
      121 ttccatgtcc gcaagttttt ctccaaacat gcatcgggtc gcgatatcca cgattggaag
      181 tccggtaacc ttgctgatat agggaacggt tcggcttgat cgggggttaa cctcgataac
      241 atatatgtca tcgcccttag cgataaactg aatgttaatc aagccaataa ccttaagact
      301 ctttgcgagc tttgccgcgt aattggccat cttttctttt tgagtaatcg ttaagtgaat
      361 gggcgggtag actgaaatgc tgtctccgga atgaataccg gttcgctcaa catgctccat
      421 gattcccggg atcagaatgt tttccccgtc gcatacggcg tcaacctcgg cttcgcagcc
      481 ttcgatatat ttgtctacca gaatcgggtt ttcttgctcg cttcgattga tgatttccat
      541 aaactcaacg atgtcatcat ctgagtacgc aatttccata ccctgcccgc ccagaacata
      601 ggagggcctg accaatacgg ggtatcccaa ttcatgagat gcatcgatag cttgttcagc
      661 ggtaaagacc gttttaccgg ccgctcgcgg tatcgctgtt tcttcaagta cctgatcaaa
      721 aagctcccgg tcttccgctc tgtccacatc acgggcgctc gttccaagga tgggcacacc
      781 catagccgcg acagccttgg cgagttttat agcggtttgt ccgccaaact gcacaacggc
      841 tcccaccggt ttttcgttgt ccacgatatc ggcaacgtct tctgcggtaa gtggctcaaa
      901 atagagtctg tcggagatat caaaatcagt actgaccgtc tccggattat tatttacgat
      961 aatcgtttca tagccgagct ctcgcaaggc ccacacacaa tggaccgtac aatagtcaaa
     1021 ctcaataccc tgaccaattc tgattggacc tgaacccagg acaaggacag tacccttgga
     1081 aggtgagccg tcttttcgcc ttg
//
LOCUS       DAOQXQ010000650         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_178659_length_1102_cov_3.603629, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000650 DAOQXQ010000000
VERSION     DAOQXQ010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178659_length_1102_cov_3.603629"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKH81_09335"
     CDS             <1..327
                     /locus_tag="PKH81_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ98278.1"
                     /translation="GKAPFFVPLLFTQSILHYHIYIMTNKNKTVLYTGVTSDLIKRVY
                     QHKEKMVEGFTKKYNITKLVYYELFDDPENAIAREKQIKAGSRQKKIDLVNTMNSSWN
                     DLYETL"
     gene            complement(330..578)
                     /locus_tag="PKH81_09340"
     CDS             complement(330..578)
                     /locus_tag="PKH81_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98279.1"
                     /translation="MAVALPWNPLKKEVSESASKICHCEESRTARDDAVIFMVCRQIA
                     SVVSLPRNDPSTVIAKSPARDEAISRHSEVVVTALHSS"
     gene            560..>1102
                     /locus_tag="PKH81_09345"
     CDS             560..>1102
                     /locus_tag="PKH81_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNQ98280.1"
                     /translation="MEEQPPYGLPAYELPDYLEKVQCQLPALHLLMQLGWEYLTPEET
                     VNLRAGRLGSTILEPLLVDHIRNHCGYDFKGATHPFTENAIQNAVQALKGFRATGAAY
                     ENEQAYDLLCLGTSVPQTVEGDTKSFTIHYIDWDQPENNTYHCTAEYKVERVGYQKHY
                     VPDIVLFVNGIPLVVIECKRS"
BASE COUNT          309 a          267 c          268 g          258 t
ORIGIN      
        1 ggaaaagcgc ctttttttgt acccttactt tttacacaat cgattttaca ctaccacatt
       61 tacatcatga ccaacaagaa caaaacggtt ctctacacag gtgtaacaag tgacctgatc
      121 aagcgggtct atcaacacaa ggaaaagatg gttgaaggat ttacgaagaa atataacatt
      181 acgaagctgg tatactatga gcttttcgac gaccctgaaa atgccatagc aagagagaaa
      241 caaattaagg ctgggtcacg acagaagaaa atcgatctgg tgaatacgat gaatagcagc
      301 tggaacgatc tttatgaaac gctttgagat tacgaggaat gaagcgctgt gacaactacc
      361 tcgctgtgcc gggagattgc ttcgtcccgc gcgggacttt tcgcaatgac ggtgcttggg
      421 tcattgcgag ggagtgaaac gaccgaagca atctgccggc acaccatgaa gattaccgcg
      481 tcgtcccgcg ccgtgcggga ctcctcgcaa tgacagattt ttgaggcact ctctgaaact
      541 tcttttttta gaggattcca tggaagagca accgccatac ggcctaccgg catacgaatt
      601 gcctgattat ttggaaaagg tccagtgcca gcttccggcg ttgcatcttc tcatgcagct
      661 cgggtgggaa tatctgaccc cggaagagac ggtgaacctt cgcgcagggc ggctgggttc
      721 gaccatcctg gagcccttat tggtcgatca catccgcaat cattgcgggt atgacttcaa
      781 aggggcgacg catccattca ctgagaacgc cattcaaaac gccgtgcaag cactcaaagg
      841 atttcgggct accggggcag cctatgaaaa tgagcaggcc tacgacttgt tgtgcctcgg
      901 aaccagcgtt ccccagaccg tagaaggaga taccaaaagc tttaccatac actatatcga
      961 ttgggaccaa ccggaaaaca acacttatca ttgtaccgca gaatacaaag tggagcgcgt
     1021 cggttatcaa aagcattatg taccggacat cgttctcttt gtaaacggta ttccccttgt
     1081 cgttattgaa tgcaagcgct ct
//
LOCUS       DAOQXQ010000651         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_179228_length_1100_cov_3.143541, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000651 DAOQXQ010000000
VERSION     DAOQXQ010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179228_length_1100_cov_3.143541"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKH81_09350"
     CDS             complement(<1..754)
                     /locus_tag="PKH81_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ98281.1"
                     /translation="MIEQRKIGTVLAAILSLSAIFAGGAFAQETDTPLVKELTIEEAV
                     NLAMENNISLASSAIDLRIKKRTDDYALNMLIPTVQLSGTLARSNNVSNPYAPLLKSI
                     NPYYQEAEVTEADHWTAMAGLSISLNLNAAIVEGLRMARKSYEAGLLTWDQARKETEK
                     NVRKAFYAILLQEGSLAIAKDKLATSEERFRQIQINYRNGLVSELILLQTQLAWETQK
                     PAIQESELGLQQQKEFFAFLLGLPAGTEVQLSG"
     gene            complement(751..>1100)
                     /locus_tag="PKH81_09355"
     CDS             complement(751..>1100)
                     /locus_tag="PKH81_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98282.1"
                     /translation="IVMAVQSNYLLVNPDLIPVFNWIRYVTFSNDYQEHEHDIDLDAL
                     TSAFHTQDLFPDGKIDVLCIIGIIKWASILSTSLTISEFKPGVAPEVIQKDIRFLYRR
                     MLGGDELVYKEKT"
BASE COUNT          243 a          259 c          279 g          319 t
ORIGIN      
        1 tcccggacag ctgtacctcg gttcccgctg gaagtccaag aagaaaggca aagaattcct
       61 tttgctgctg caagccaagt tcgctttcct gaatcgccgg tttttgagtt tcccaggcga
      121 gttgggtttg taagaggata agttcgctca ccaaaccatt tcggtaattg atttggattt
      181 ggcggaaacg ttcctcgctt gttgcaagtt tgtctttcgc gatagcaaga gaaccttcct
      241 gaagcagtat ggcgtaaaag gccttgcgta cgtttttttc tgtttccttg cgtgcctgat
      301 cccaggtcaa taggcctgct tcataggatt ttctggccat cctgagccct tccactatcg
      361 ccgcgttcag gttaaggcta atcgaaagcc cggccatagc ggtccagtga tctgcttcgg
      421 taacctcggc ttcctggtag taagggttaa tgcttttaag aagcggcgcg tacggattgg
      481 acacgttgtt ggaccgcgcc agtgtgccgg aaagctgcac ggtcgggata agcatgttaa
      541 gggcataatc gtccgtgcgc tttttaatgc gtaaatctat cgcgcttgat gcaagggata
      601 tgttgttctc catcgccagg ttaaccgctt cttctattgt caattcctta acaagaggag
      661 tgtctgtttc ttgggcgaag gcccctccgg caaaaattgc actcagactg agtatggcgg
      721 ccagtactgt ccctatcttt cgttgctcta tcatgttttc tccttgtata ccaattcgtc
      781 tccgcccagc attcgccggt acagaaaacg gatgtctttt tgtattactt ccggtgcaac
      841 accgggttta aattcactga tggtaagact ggtggataat atcgatgccc atttgattat
      901 cccgatgatg cacaggacgt cgatcttccc gtcgggaaaa aggtcttgcg tgtggaaggc
      961 actcgtcagg gcatcaaggt caatgtcatg ctcatgttct tgatagtcgt tgctaaaggt
     1021 tacatagcgg atccagttaa aaaccggtat caggtccgga ttgaccaaga ggtagtttga
     1081 ttggaccgcc ataacgatat
//
LOCUS       DAOQXQ010000652         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_180040_length_1097_cov_3.210173, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000652 DAOQXQ010000000
VERSION     DAOQXQ010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180040_length_1097_cov_3.210173"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..366)
                     /gene="infA"
                     /locus_tag="PKH81_09360"
     CDS             complement(148..366)
                     /gene="infA"
                     /locus_tag="PKH81_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNQ98283.1"
                     /translation="MAKEEAIEVEGVVKESLPNTMFRVELQNGHVILAHLSGKMRKHY
                     IRIVPGDKVKVALSPYDLNRGRIIYREK"
     gene            475..831
                     /locus_tag="PKH81_09365"
     CDS             475..831
                     /locus_tag="PKH81_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNQ98284.1"
                     /translation="MEKDFAEKMKKSLYELKKEIIDTLISNNEDFRAIVEGIDPKDFA
                     DIASDDMDRKMLETIGSKDMKRMRLIDSAITRIEQGKYGLCMKCGKKIPKARLEAIPY
                     ALMCIECQSADERRNR"
     gene            complement(842..>1097)
                     /locus_tag="PKH81_09370"
     CDS             complement(842..>1097)
                     /locus_tag="PKH81_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNQ98285.1"
                     /translation="IAKTDKVVEINTGGIARKAIDDVYPSSRLLTLLCQAGVKITLSS
                     DAHAAEDLDTGYEKARQSALKAGFTGLWFFEGDGWTQEAL"
BASE COUNT          281 a          223 c          264 g          329 t
ORIGIN      
        1 atccttacct ttttggttgg ggtgttgtta ttgcgagtgt cttttgtgtt tggtattttt
       61 ggtttgatta tctgccttac ggcgattgga aggggttttg ttaattcact aaaggcactc
      121 aggcttttat ttagcctgag gcagtaatta tttttcccgg taaataatcc gtccgcggtt
      181 aagatcgtag ggagacagcg caacctttac cttgtctcca ggaacaattc gtatatagtg
      241 cttgcgcatt tttccggaca ggtgcgcaag gataacgtgt ccattttgta gctctactct
      301 aaacatggta ttgggcagtg attcttttac cacaccctct acttcaatag cttcttcttt
      361 agccacaatg cctccaaaaa gtaagagaaa agcgcagaaa tagtttgatt tttgcggatt
      421 tcgttcgtat aattctaggg tctatcataa cctttgtcaa ctggagcttg ttgtatggaa
      481 aaagatttcg ccgagaagat gaaaaaatcg ctttacgaac ttaaaaaaga aataatcgat
      541 acgctgatat caaataacga ggattttcgc gctattgttg agggcattga tcccaaggac
      601 tttgcagata ttgcctcgga tgatatggac aggaagatgc tcgaaactat tgggtccaag
      661 gacatgaagc gaatgcgttt gatcgattca gcgattaccc gaatcgagca gggaaagtac
      721 ggtctttgta tgaagtgcgg aaaaaaaata cccaaggctc gactcgaagc aattccgtat
      781 gccttgatgt gtatcgagtg tcaaagcgca gacgaacgga gaaaccgcta gcgatttgtt
      841 cctataaggc ttcttgcgtc cacccatcgc cttcgaagaa ccaaaggccc gtgaatcctg
      901 cttttagtgc ggattgacgg gctttttcgt atccagtgtc caagtcttct gcggcatggg
      961 catcagagct aagggttatc ttgactcctg cctggcagag cagggttagt aaacgactag
     1021 agggatatac gtcatcaatc gcttttcggg caatgcctcc ggtgttaatc tcgaccacct
     1081 tgtctgtttt ggcgatg
//
LOCUS       DAOQXQ010000653         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_181176_length_1093_cov_3.161850, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000653 DAOQXQ010000000
VERSION     DAOQXQ010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181176_length_1093_cov_3.161850"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKH81_09375"
     CDS             complement(<1..435)
                     /locus_tag="PKH81_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HNQ98286.1"
                     /translation="MVALISAGSQKGHAMIECTSLEEVRASIDSIDSKIVEKVYRAMI
                     ESFINRELTELKKKKEPSMSTPNLPLAKIDYDQYFSVSNANNKEFSVLELGDLAITSG
                     KIIAADPLVFLGKVSPFTQTIKPGKYPVKMCVYDTDMGSGYAF"
     gene            613..>1093
                     /locus_tag="PKH81_09380"
     CDS             613..>1093
                     /locus_tag="PKH81_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98287.1"
                     /translation="MGKTIREIFMMKKFLLLLVCLVMCGTSIWAQNKTKDTRNFRLPG
                     GDYFFGFSATDPREFQLFYIDDSGSVTMINPGLLPWINTKHSSIRILYPGDKLVWASS
                     VNEEGVDTYGNATILRAGNNTTGKFTVLDTCSKDRDITLVDLSPDGKNILYKVLYPEY
                     "
BASE COUNT          294 a          229 c          228 g          342 t
ORIGIN      
        1 aaaggcgtat cccgatccca tgtctgtatc gtatacacac atcttaaccg gatattttcc
       61 cggctttatt gtctgcgtaa aaggcgatac ctttccaagg aatactaatg gatccgctgc
      121 aataatcttg ccgctggtaa tagcaaggtc gcctaattcc agaacagaga actctttgtt
      181 gttagcgtta cttacagaaa agtactggtc gtagtcgatc tttgctagtg gcaggtttgg
      241 tgtggacatg gaaggctcct ttttcttttt gagttcggtc aattcccgat ttataaagga
      301 ctcgatcata gctctataca ctttttcaac aatttttgaa tctattgaat caatgctcgc
      361 tctaacttct tcaagtgatg tacattcaat catagcatgt cctttttggc ttcctgcaga
      421 tattaatgct accatccata tgtatttcgt acaagggttg acagctcaaa gagaccgaac
      481 ttacaatggt ttattaccgt accatcgtgt tggcacaggt agcggagtgg acggaacctt
      541 ttccttcctg gaatttggcg aaactaaaaa tttttttgta gcagacaacg tactggtatt
      601 tatgttgaat aaatgggaaa aacaataaga gagattttca tgatgaaaaa gtttttgctg
      661 cttctggttt gccttgtaat gtgcggaacc tctatctggg cccagaacaa aactaaagat
      721 acgcgaaact tccggttgcc gggcggagat tatttttttg gtttttccgc taccgatccc
      781 cgggaatttc aattattcta cattgatgac agcggttctg tgactatgat taacccgggg
      841 ttactccctt ggataaacac caagcattcc tccatcagga tcctttatcc cggagataag
      901 ctcgtctggg ctagcagcgt caatgaagaa ggggtagaca catacggtaa tgcaacgatt
      961 cttcgtgccg gcaataatac gactgggaag tttactgtac tggatacctg ttccaaagat
     1021 agagatatca cccttgttga cttatctccc gacggtaaaa atattcttta caaggttctt
     1081 taccctgaat att
//
LOCUS       DAOQXQ010000654         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_181331_length_1093_cov_2.027938, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000654 DAOQXQ010000000
VERSION     DAOQXQ010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181331_length_1093_cov_2.027938"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKH81_09385"
     CDS             complement(<1..231)
                     /locus_tag="PKH81_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98288.1"
                     /translation="MRVKFYLIVLLAGLASCSSEKKKPVSIQSWITTPDRTKQLTQQP
                     DLVFGSEAGTGLTIEVDTSRTYQSIDGFGFALT"
BASE COUNT          336 a          227 c          229 g          301 t
ORIGIN      
        1 ggttaaagca aaaccaaatc catctattga ttgatacgtt ctggaagtgt ccacttctat
       61 ggtaagacct gtgcccgctt ccgatccaaa aacaaggtca ggttgctgtg ttagttgttt
      121 ggtccgatca ggtgtggtta tccacgattg aatactaacc ggtttctttt tttccgatga
      181 acaggaggct agccccgcaa ggagcacaat caggtaaaat tttactcgca taattttaaa
      241 gatacaaaag acacaacgaa ccagaaataa aaaacacctt ctgattgagg cttttcaacc
      301 agtagaagaa tttctgcttt tgcctggtta aaagaccaga agcacgttaa aaataaatac
      361 caagggataa tgctattgtc gataacgaat aagcgcgaga cagagaactc cgaaggagtg
      421 gtattgcatc accttttcag agttttgttt aacgatttat agaatagcca aaaaaccaat
      481 ctcatcggga tttcctcaag ataccagtgt attccctcca caatctgata aaaacattta
      541 agcagaacaa atcccgaagg gatgatagta ttgtagataa cgaataaagc atgaaacagt
      601 gaactccgaa ggagtgacat tatatcaccc cttcagggtt ttgtctttga agccgccacc
      661 ttttggaatt tgtatgacgg catctttgtc gctgccacgg tcggatacat actctctatt
      721 ctccgcagag tctcgcctcg gactctgcgg aatatgggta gtgtcttgac gcagggtccc
      781 cgggcggccg actctgagaa gaaaggaaga catgagcaaa tcatttgcgg gtttgatgtt
      841 gttctatgcc atgggcatga cattaacatg aaagatacgc tcaaggcttt tccctgttaa
      901 actaaattca gtacaagact atgcaaaacg agtatcgatg gtaagtctgg ctgtttaagt
      961 tggctaatta acgagaagca aaactaagag agaacacact tccttttcca ggaagtgatc
     1021 ttacacttat atcacctttc tgtttttgca taatttgctg gcagatactc aaaccaatgc
     1081 ctgagccact ttt
//
LOCUS       DAOQXQ010000655         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_181523_length_1092_cov_2.502411, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000655 DAOQXQ010000000
VERSION     DAOQXQ010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181523_length_1092_cov_2.502411"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKH81_09390"
     CDS             complement(<1..842)
                     /locus_tag="PKH81_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNQ98289.1"
                     /translation="MKREEYIGDAEWHRFLEFSEKLETPCVVVNLRTVKKNYIKLQNS
                     FPFAQIYYAVKANPAPKVISLLSELGSNFDIASRYELDKVLALDVEPERISYGNTIKK
                     SKDIEYFYEKGIRMFATDSKEDLKNIAKHAPGSKVYVRILVENSVTADWPLSRKFGCH
                     PDMAYDLLVQARDAGLTPYGISFHVGSQQRDIGQWNDAIAKTKYLMTSLEEEEGLRLQ
                     MINMGGGFPANYTQPTNDLSEYASEITRYLTEDFGDELPLIVLEPGRSLVGDSGILVT
                     EVVL"
BASE COUNT          250 a          256 c          296 g          290 t
ORIGIN      
        1 ataaggacta cctcggttac aaggattccc gaatccccaa cgagcgagcg gccgggttca
       61 aggacgatca gtggaagctc gtctccaaag tcctcggtaa ggtagcgggt aatctcgctt
      121 gcgtactcgg agaggtcgtt ggtgggctgg gtgtagttag cggggaagcc gccgcccatg
      181 ttaatcatct gaaggcgaag gccctcttct tcttccaggc tcgtcataag gtacttggtc
      241 ttggcaatgg cgtcgttcca ttggccaatg tcgcgctgtt gactgcctac atggaaggaa
      301 atgccgtagg gggtaaggcc ggcatcgcgg gcttgcacca aaagatcgta ggccatgtcg
      361 ggatggcaac cgaatttgcg ggaaaggggc cagtcggcgg taacggagtt ttctaccagg
      421 atgcgcacat acactttgga gccaggggcg tgcttggcta tatttttaag gtcttcctta
      481 ctgtccgtgg cgaacatacg gatacccttt tcataaaagt actcgatatc cttggatttc
      541 ttgatggtgt tgccgtagct gatgcgctca ggctctacgt ccagggcaag aaccttgtcc
      601 agttcgtagc gggatgcgat atcgaagttg gatccgagct ctgaaaggag cgagattacc
      661 ttcggtgccg ggttggcttt gaccgcgtaa tagatctgcg cgaacggaaa ggagttctgc
      721 agcttgatgt aattcttctt aacggtccgt aaattgacca ccacacaggg tgtttcgagt
      781 ttttccgaaa actcgagaaa acggtgccat tcggcatcac cgatgtattc ttctcttttc
      841 aattagtttt attgcccctc ccggatctct aaaaagtgat ttttgcaata taaataccct
      901 gaccgattgt gtcaatcggc aatatttacg ataattgtaa aactatggaa acccttatcc
      961 agattggcgc attattcatc ttgatggcag taggctttgc ccttggtaag cttaaaatcc
     1021 tggaagaggc agcgataaaa gggctctctg ccctggtaat caaggctgcc ctacccgcct
     1081 tgatcctgat gt
//
LOCUS       DAOQXQ010000656         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_182125_length_1090_cov_2.285024, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000656 DAOQXQ010000000
VERSION     DAOQXQ010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182125_length_1090_cov_2.285024"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKH81_09395"
     CDS             complement(<1..573)
                     /locus_tag="PKH81_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ98290.1"
                     /translation="MNFKRTLRLIAVSLSAIACLALVAWFSTCSPGSAQTAFADSPVK
                     TEIPADSIALLEGMQKANRAVSQSILPAVVTLDVIETRTVRSNPLDGFPWFFFGNPQD
                     EKGKEQNGDSEEAPKREYKAEGLGSGVIIRKVGNTFYLLTNQHVAGAANEINVKLYDG
                     REFTGKLIGGDERKDIALVSFESNDSNETRA"
     gene            complement(675..>1090)
                     /locus_tag="PKH81_09400"
     CDS             complement(675..>1090)
                     /locus_tag="PKH81_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ98291.1"
                     /translation="KDALDLLAVTGNCLSAGISACIAGNRVSDISRAVYNTAVSSGYG
                     VVWEYCGHGVGLAVHEDPQIPNVPERGANPRIVPGMVLAIEPMINMGSGDVDVLDDNW
                     TVVTQDRKVSCHMEHTVAVFPDHTEVLTQLDPAED"
BASE COUNT          314 a          267 c          245 g          264 t
ORIGIN      
        1 tgctctggtt tcgttcgaat catttgattc aaacgaaacc agagcaatat ccttgcgctc
       61 gtcaccaccg atgagcttgc cggtaaattc acgcccatca tataatttga catttatttc
      121 gttcgcggca cccgcaacat gctgattggt taacagatag aaggtattgc cgaccttgcg
      181 gataataaca ccggaaccaa gtccctctgc cttgtattct cttttaggag cctcttcact
      241 gtccccattc tgttcctttc ccttttcgtc ctggggattt ccaaagaaaa accagggaaa
      301 gccatcaagt ggattggaac gaacggttcg ggtttcaata acatcaaggg ttaccacagc
      361 gggtagaatc gactgggaaa cagcgcgatt tgctttttgc attccctcaa gaagggcaat
      421 agagtcagcc ggtatttcgg tctttaccgg agaatctgca aaagctgttt gcgccgaacc
      481 gggcgaacag gtagaaaacc aggcaaccag agccaaacag gcaatagctg acaaggaaac
      541 ggcaataagt cttaacgttc ttttaaagtt catgaataca cctccaggag aaggaacgat
      601 tgctctttcc ttaatgtaat aaaaaaaaag ctcttgtggt tacacagtag agaaaaaaag
      661 atgtacagga tacatcaatc ctctgcagga tcaagttggg taagaacttc tgtatgatcc
      721 ggaaatacgg caacggtatg ttccatatgg caggaaacct tgcggtcttg agtaacgaca
      781 gtccagttat catcaagaac atcaacatca ccgctaccca tatttatcat cggttcaatc
      841 gcaagaacca ttcctggtac tatacgcggg tttgcaccac gttcgggaac atttggaatt
      901 tgcgggtctt cgtgaacggc aagaccgacg ccatgtccac aatactccca gacaacaccg
      961 tagccggaac tgacagccgt gttatacaca gcccgggaga tatcggatac tcgattaccc
     1021 gcaatacacg cggagatacc tgcgctcagg cagttgccgg taacagccaa aagatcgagc
     1081 gcatctttgg
//
LOCUS       DAOQXQ010000657         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_183227_length_1086_cov_3.175558, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000657 DAOQXQ010000000
VERSION     DAOQXQ010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183227_length_1086_cov_3.175558"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKH81_09405"
     CDS             <1..442
                     /locus_tag="PKH81_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ98292.1"
                     /translation="GDAKLAQEVVKTSLSTDQLYAIGRSLSSATTKGDILAAELSGDY
                     SSELLSVIADFLLRLREVSFVLVIEVRGEEYRLSARNRDPRLDAGVIVARALEGIGTG
                     GGHPHMAGGLIKPEHYPGAQSLLDMIAQEIDRARSSNDAKPTKN"
     gene            417..>1086
                     /locus_tag="PKH81_09410"
     CDS             417..>1086
                     /locus_tag="PKH81_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HNQ98293.1"
                     /translation="MTQNLRKIELEGRQILLLGTAHVSRESVEEVAQAIREEKPDVVC
                     VELDEARLATLKNNKGWQELDITKVLKEGKGFLLLANLVLSSFQRRIGNDTGSKPGDE
                     MKKALEVAEEEGIRTAMVDRPIHVTLKRAWSKNSFIGKVKLLAALTGSAFTSEELTEA
                     DIEALKNKGAMEEMMNELADYLPTVKEVLIDERDRYLASRIWSAGGTKSLAVLGAGHL
                     AGTEG"
BASE COUNT          289 a          267 c          319 g          211 t
ORIGIN      
        1 tggagacgcg aaactcgcac aggaggtagt aaagacgtcc ctgagcaccg accagctcta
       61 tgccattggg cgctctcttt cctcggcgac taccaagggc gatatccttg ccgcggagtt
      121 atcgggcgac tattcgagcg agcttctttc cgtaatcgcc gattttctgc tacgattgcg
      181 cgaggtatcc tttgttctgg ttattgaagt tcggggggag gaataccgac tgtccgcacg
      241 aaaccgggac ccccggctag acgccggagt aattgtcgcc cgagcccttg agggcattgg
      301 aactggaggc gggcacccgc atatggcagg tggcctgatt aaacctgaac actacccggg
      361 agcgcagtcg cttctggaca tgatagcaca agagatagac agagcaagga gttccaatga
      421 cgcaaaacct acgaaaaatt gagctggagg gtcgacagat actccttttg ggaaccgccc
      481 acgtatcccg cgaaagcgta gaagaggtgg cacaggcgat tcgtgaggaa aaaccggatg
      541 tagtgtgtgt ggaacttgac gaagcacgcc ttgcaactct aaaaaacaac aagggatggc
      601 aggagcttga tattaccaag gtgcttaaag aaggcaaggg gttcttgctt ctggcaaacc
      661 tggtgctctc ttcgttccaa cgacgcatcg gcaacgatac cggaagcaaa cctggcgacg
      721 agatgaaaaa ggccctggaa gttgccgagg aagagggaat tcgcaccgcc atggtagacc
      781 gccccatcca tgtaaccctt aaaagagcct ggtccaaaaa cagctttatt ggcaaggtaa
      841 aactccttgc cgctcttacc ggcagtgcct ttacaagcga agagcttacc gaggcagata
      901 tagaagccct aaagaacaag ggcgcgatgg aagagatgat gaatgaactt gccgactacc
      961 ttccgacggt aaaggaagtg ttgatagacg agcgggatcg ctaccttgca agcaggatat
     1021 ggagcgccgg cggtacaaag tccctggcgg tgttaggggc cgggcattta gccggaacgg
     1081 aaggta
//
LOCUS       DAOQXQ010000658         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_183484_length_1085_cov_3.348544, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000658 DAOQXQ010000000
VERSION     DAOQXQ010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183484_length_1085_cov_3.348544"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PKH81_09415"
     CDS             complement(<1..804)
                     /locus_tag="PKH81_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98294.1"
                     /translation="MTRRHSTFFFFVFCLVVVSSCSTRYSRVPCTGAPHDKLIALTDA
                     ARDGVLTGPSSRAWFSFGDSALESAQERAIEVMVSAEGSEPVTISLAPVFASDVQHNG
                     SLSSVLSGRHISRLEGLSGTIKLSMIPFDDKQRIAGFAVERAGGGDTRVTITSVSVRQ
                     ARTGWDVREGERWVGFGPEGGSARLGSLPESFPLEVPSHATMELVISPVPPSDAGIPT
                     KPTRFSLVSRDTAFGFRSSPVPHIALVSGSLPQVSSGVLSFEPANPPVQA"
     gene            complement(801..>1085)
                     /locus_tag="PKH81_09420"
     CDS             complement(801..>1085)
                     /locus_tag="PKH81_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HNQ98295.1"
                     /translation="HYLADLGASIVREDQFHYMVSAANFIKSACSALFMENGTFEPSH
                     RGFLSQIQDLKTLPDDFLGRFESFLRPDSELPPARKYEIAKLIARSIVSL"
BASE COUNT          311 a          273 c          306 g          195 t
ORIGIN      
        1 tgcctgaacc ggtggattgg caggctcaaa cgaaagaacc ccagaagaca cctggggaag
       61 cgagccgctt accagagcaa tatgcggaac cggggaggag cgaaagccaa aggccgtatc
      121 cctggaaaca agagaaaagc gtgtgggttt tgtggggatt ccggcatcgg aaggcggtac
      181 cggagagatg acgagctcca tagtggcatg ggagggaact tcgagcggaa aactttctgg
      241 aagtgagcca agtctggcag agcccccttc gggtccaaag ccaacccagc gctcaccttc
      301 gcggacatcc catccggtgc gggcttgacg aacagacacg ctcgtaatgg taacccgtgt
      361 atccccgcct cctgcacgct caacagcaaa acctgcaatc cgctgcttat catcaaacgg
      421 aatcatggat agcttgatag taccactgag cccttcaaga cgggaaatat gacggccgga
      481 aagaacggat gaaagggatc cattgtgttg cacgtcagag gcaaaaaccg gagccagaga
      541 aatggttacc ggctcgctac cttcggcgct taccataacc tctattgccc gctcttgagc
      601 gctctccagg gcgctgtcgc caaaggaaaa ccaggcccgc gaagaagggc cagtaagcac
      661 tccatcccga gcggcgtcgg ttaaggcaat gagcttatcg tgtggagcgc cggtacaagg
      721 cacgcgggaa tagcgggttg aacaggagga tacgactaca agacaaaaaa caaaaaagaa
      781 aaaggttgaa tgtcgtcgtg tcataaactc acaatacttc gggcaataag cttggcgatc
      841 tcgtatttgc gtgcgggcgg cagttcagaa tctggcctga gaaagctttc gaagcggcca
      901 agaaaatcgt cgggcagggt ttttagatcc tgaatctgtg aaagaaaccc acgatgcgat
      961 ggctcgaatg taccattttc cataaagagg gccgaacacg cactcttgat aaaattagcc
     1021 gcagagacca tgtaatgaaa ctgatcctcg cgaacaatag aggcgccaag gtctgccaaa
     1081 taatg
//
LOCUS       DAOQXQ010000659         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_184371_length_1082_cov_2.834469, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000659 DAOQXQ010000000
VERSION     DAOQXQ010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184371_length_1082_cov_2.834469"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1082
                     /locus_tag="PKH81_09425"
     CDS             39..>1082
                     /locus_tag="PKH81_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ98296.1"
                     /translation="MLVTLINEMSASILAKNSQLLEANTLLEGRVAERTTELSKSLAE
                     LRQAQDRLIESGKLSALGLLSAGMAHELNTPLGAILSSNRNVIDFFDSKMQGFRDFFL
                     SLSDREYALYTNALAQGGALSRSLDVPEVDRSRQKEIRAILEERGVQDSRAVTDYLVD
                     LGLDPDDSVLGPLLVENRAVEVLTAVSDLLITRRMAEIVDIAGKKAATVVSALRFYLS
                     TDHEDMQATVDIEQDIDKILTLMHNMLKHGVTIKREYSGVRTKGSSDKLGQVWMNILR
                     NAAQAMDFKGEIIIRTQTQDALTIVSFIDSGPGIPDDILPRVFDPFFTTKKQGEGMGL
                     GLDICRRIIESHNG"
BASE COUNT          289 a          275 c          267 g          251 t
ORIGIN      
        1 cctcgcgtat aaaggggtca gaatatacag acgtaaacat gcttgtcacc ctgattaacg
       61 agatgtccgc aagtatactt gccaaaaact cacagctcct ggaagcaaac accctgcttg
      121 aaggacgagt ggccgagcga accaccgagc tcagtaagtc ccttgccgaa ttgcgacaag
      181 cacaggatcg ccttattgaa tcgggaaaac tatcggcact cggcttgctg tccgcaggaa
      241 tggcgcatga acttaacacc ccacttgggg ctatcctgtc ctcaaaccgg aatgtcatcg
      301 acttttttga ctccaagatg caaggctttc gtgatttttt cctctcgctc tccgatcggg
      361 agtacgccct gtataccaat gcactcgctc agggaggagc cttgagccga agccttgatg
      421 taccagaggt tgatcgctcg cgtcaaaagg aaattcgcgc aatcctggaa gaaaggggcg
      481 tgcaggacag ccgggcagtg actgactatc ttgttgatct gggccttgat cccgatgata
      541 gcgttcttgg gcccctctta gtcgagaacc gagccgtaga ggttcttacg gcagtatcgg
      601 accttttgat aacacgtcgc atggcagaga ttgtggatat cgcgggaaaa aaagctgcta
      661 ccgttgtctc cgcacttcgt ttctaccttt ctacagatca tgaggatatg caagcgaccg
      721 tggacattga acaggatatt gataaaatcc tgacccttat gcacaacatg ctaaagcacg
      781 gtgtaaccat taaacgggaa tactcaggcg tccggacaaa gggatcatcg gacaaactcg
      841 ggcaggtatg gatgaacata cttcgtaatg cagctcaagc tatggatttt aaaggagaga
      901 tcatcattag gacacagaca caagacgccc tcaccattgt gtcattcata gactcaggac
      961 ccgggatccc cgacgatatt cttccccggg tatttgatcc cttttttacc accaaaaaac
     1021 aaggagaagg catgggcctg ggccttgata tctgccgcag gattattgaa tcccacaatg
     1081 gg
//
LOCUS       DAOQXQ010000660         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_185603_length_1078_cov_2.577713, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000660 DAOQXQ010000000
VERSION     DAOQXQ010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185603_length_1078_cov_2.577713"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1078)
                     /locus_tag="PKH81_09430"
     CDS             complement(<1..>1078)
                     /locus_tag="PKH81_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ98297.1"
                     /translation="ELHDYFFELARQADRKGETLAVFLDRIADIRDNGDKIEGIQVPV
                     ERTGGVSIMTIHKSKGLEFPVVFLVDCANAGKNKGNDQSVYMSDRWGITINIGLKGKG
                     KAVSDNPFFLEGKEEERKKNLAELRRLLYVAMTRAELRLYISAQCSCKRDPIPDTEAT
                     ADELRHIMTMIHQESLDKISSSFLSLLSLPLSIQVLPQIRMREVLPAPRDYARQRNRD
                     ASQAATSFPEQAFISLATLPATEYPRPPKRRFTATGLPQSVETEAQKFSAFQHEQESD
                     TESDELSRALEAEGLNSAEFGTLAHKSIEAYLTNTPFTGPQGLASILHAMTQRFIDSH
                     LGQLARDSEWRESEFAFITRMRDG"
BASE COUNT          224 a          263 c          279 g          312 t
ORIGIN      
        1 tctccatcgc gcatacgggt gataaacgca aactcacttt ctctccattc actgtccctt
       61 gcaagctgtc ccaggtgtga gtcgataaaa cgctgagtca tcgcgtgtag tattgatgca
      121 aggccttgcg gtccggtaaa cggggtgttg gtaagatagg cttcgattga tttgtgggca
      181 agggttccaa attccgcact gttgagcccc tctgcctcca gcgccctgga aagttcatcg
      241 ctttcagtat ccgattcctg ttcatgttga aacgcggaaa atttttgtgc ctctgtttct
      301 acgctctgtg gaagtcctgt cgcagtaaac cgccgtttgg gagggcgggg atattctgtt
      361 gcgggcagtg tagcaagcga gataaacgct tgttctggaa aacttgttgc tgcttgcgag
      421 gcatccctgt ttcgttggcg agcgtagtct cttggtgcgg gaagcacttc cctcatcctt
      481 atttgaggta acacctgaat tgaaaggggt agagatagta atgagagaaa ggaactgctt
      541 attttatcca gggattcttg atggatcatc gtcatgatgt ggcgaagttc atctgcggtc
      601 gcttctgtgt cgggtatcgg atcccgttta catgaacatt gggccgagat atacaacctt
      661 agttccgcac gggtcattgc aacatagagc aggcggcgca gttcggcaag attttttttg
      721 cgctcttctt ctttgccttc aaggaaaaaa ggattgtccg agacggcctt tccctttccc
      781 ttgagtccaa tgttaatggt aatcccccag cggtcgctca tgtacacgct ttggtcgttg
      841 cccttgtttt ttccggcatt tgcgcaatcg acgagaaata ctaccgggaa ctccaacccc
      901 ttgctcttgt gaatagtcat gatgcttacc ccgccagtgc gttcaacggg tacttgtatt
      961 ccttcgatct tgtctccatt gtccctgata tccgcgatac ggtccagaaa cacggcgagg
     1021 gtttcgccct ttcgatcggc ttgtcgggca agctcaaaga agtagtcatg aagctccg
//
LOCUS       DAOQXQ010000661         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_185732_length_1078_cov_1.834800, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000661 DAOQXQ010000000
VERSION     DAOQXQ010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185732_length_1078_cov_1.834800"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKH81_09435"
     CDS             complement(<1..306)
                     /locus_tag="PKH81_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98298.1"
                     /translation="MIKLDSLSFRYAEADDYALCDINLDIGKGEFVCIIGAERSGKTT
                     LFRLLNGTAPRAFEGTVSGSLSVDGQDPSLRGHMATGEAVTSIFDDPDSQIISLTVAE
                     "
     gene            complement(303..851)
                     /locus_tag="PKH81_09440"
     CDS             complement(303..851)
                     /locus_tag="PKH81_09440"
                     /inference="COORDINATES: protein motif:HMM:NF028409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HNQ98299.1"
                     /translation="MSAQSEKSHMGTRDIVLAGILLAAGAVIRMFFPKLPVTPNFIIA
                     MYCLVILLVRPRVMEALAIGIVAAILSQLTSGSPVPFLNFISEPVGALVCYLLVRPKY
                     RLSIGSYSLKPAFATLLSTLASGAVFVLILSLVLMSMGKDAKYIMIATVVVPTALANT
                     VITQLAFEPLRRVLRIKDKDAQ"
BASE COUNT          289 a          255 c          296 g          238 t
ORIGIN      
        1 ttccgcaacg gttaagctga taatctgtga atccggatcg tcaaatatac tcgtaaccgc
       61 ttctccggtg gccatgtgtc cgcgaagaga agggtcttgt ccatctaccg acagagatcc
      121 actgacggtt ccctcaaagg ctcggggcgc agtaccgtta agaagtctaa aaagagttgt
      181 cttgcctgaa cgttctgccc cgattatgca gacaaactcg cctttcccga tatccagatt
      241 gatatcacag agcgcgtaat cgtctgcctc ggcatagcgg aaactgaggg aatcgagttt
      301 gatcactgcg cgtccttatc cttgatgcgc agaacgcggc gcagtggttc aaaggccaat
      361 tgggtaatga cggtattggc aagggctgtg ggaacgacaa ccgtcgcgat catgatgtat
      421 tttgcgtcct ttcccatgga cataaggacg agagaaagaa tcagaacaaa gactgcgcct
      481 gaggcaaggg tggaaagcag ggttgcaaag gccggtttaa ggctatagga gccaatcgaa
      541 agccggtatt tgggcctgac aagaaggtaa cacaccaggg cgccaacggg ttcagagata
      601 aaattaagaa agggaacggg cgagccggag gtaagctggg aaaggattgc cgcaacaata
      661 ccgatggcga gcgcttccat tacgcggggg cgaacaagca aaataaccag gcagtacatt
      721 gcaatgataa agtttggggt aaccggcagt tttggaaaga acatgcggat tactgctccg
      781 gcggcaagaa ggattcccgc taatacgatg tcgcgggtgc ccatgtgact tttttctgat
      841 tgagcgctca taatagtctc catacaaaaa ataaaaattc gggcccctag cggtccgtct
      901 gaacagatac tacgccttgg gcttgtttct gtcaatagaa acacggccgg agcattgttt
      961 ttttcaaaaa aaaattattc caggctttcc agttcttttt tccagatcgc ggccgaggca
     1021 tcgcttggca tacgccagtc gccacggggg gaaaggcttc cggacccacc cttgggcc
//
LOCUS       DAOQXQ010000662         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_185911_length_1077_cov_2.707436, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000662 DAOQXQ010000000
VERSION     DAOQXQ010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185911_length_1077_cov_2.707436"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="PKH81_09445"
     CDS             <1..>1077
                     /locus_tag="PKH81_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ98300.1"
                     /translation="RDILDSVSLNLQAGTKAALAGANGSGKSTLMKVLSGEIQCDSGE
                     RAIQKDTRVSYLPQSGIVHSGKTLRDEIDSAFSRGYELLRAFEELGDQLAHESADSPR
                     LDRLISEHHDIGQALEDSQWHRRSALAEQTLYGLGFAPSDLDRNVSEFSGGWQMRIAL
                     AKILLERPDILLLDEPTNYLDLEARTWLETWLREYPGGFLIVSHDRFFLDSTINEVYE
                     LFSGQLKRYPGDYSHYEQVRQVELASLIKRYEEQQEEIAKTEDLIRRFRYKATKAAMV
                     QERIKKLEKMERIEIPESLKKIHFTFPPAPPTGRLVVTLSGLTKGYGERVVIQGLDAV
                     IEKGERLVVVGRNGAGKSTLLRMIA"
BASE COUNT          268 a          267 c          281 g          261 t
ORIGIN      
        1 agggatatcc ttgattcagt atcgcttaat cttcaggcgg gaactaaagc cgccctggcc
       61 ggggcaaatg ggtcgggaaa atcaaccctg atgaaggttc tttctggaga gattcaatgc
      121 gattcggggg agcgggctat tcaaaaagac acccgcgttt cctatcttcc tcagtccggc
      181 attgttcatt caggaaaaac gctccgcgac gagattgaca gtgcatttag ccgcgggtat
      241 gaattgcttc gcgcatttga agagcttggg gaccagcttg ctcacgaaag cgccgattca
      301 ccccgccttg acaggcttat cagcgagcat cacgacattg ggcaagccct agaggattct
      361 caatggcatc gccgttcggc cctcgccgaa caaaccctgt atggcctcgg gtttgctccc
      421 tccgatctgg accgaaatgt gtcagagttt tcgggcggat ggcaaatgcg tatcgctctt
      481 gccaagatat tgcttgaacg accggatatc cttctgttgg atgagccgac taactacctt
      541 gatcttgaag cgagaacctg gctcgaaacc tggctccgcg aatatccggg cggttttcta
      601 atcgtaagcc atgatcgatt ttttttggat tccacgatta acgaggtata tgaactgttc
      661 tcagggcagc tcaaacgcta tcctggcgac tattctcact acgaacaggt tcgacaggtt
      721 gagcttgcct cgcttatcaa gcgatatgaa gaacaacaag aagagattgc aaaaaccgaa
      781 gacttgatcc gacggttccg ctataaggct accaaggccg ccatggttca ggagagaatc
      841 aaaaaacttg aaaaaatgga acggattgaa attccggaaa gccttaaaaa aatccatttt
      901 acctttcctc ctgccccgcc aaccggtcgt cttgtggtaa ccctgagtgg tttgaccaag
      961 ggctatggcg aacgtgtcgt tattcaaggt ttggacgcgg ttatcgagaa aggcgagcga
     1021 ctcgttgtcg tcggtcgtaa cggggcggga aagtctacct tgctcaggat gatcgcc
//
LOCUS       DAOQXQ010000663         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_186150_length_1076_cov_3.375122, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000663 DAOQXQ010000000
VERSION     DAOQXQ010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186150_length_1076_cov_3.375122"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKH81_09450"
     CDS             <1..1024
                     /locus_tag="PKH81_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNQ98301.1"
                     /translation="SYRKLFISKTPISSEVLYAVTFDNTMGVVHSSNRQWTSVTFGSC
                     PSLVRPFVNTYLMLDGTPFTNQTDYATKSFPQECVNRDSRLAQTVRTPGFTRITGGTT
                     VKTAPNYAYAITGYQTCKFTLDDTQFDNVDICDNNVILFRYAEVLLNYAEAKAELGSL
                     TDADWAKTVGALRARAGITGGISSKPVAVDPYLKSTFFPDVSNPEILEIRRERGIELS
                     FEGFEWSDVCRWKLGNSLTRDWEGIYIPEFEVPYDMNGDGTLDVCFTKSLTPANKVPG
                     VYYLYVGEKLANGAKNNSQIAADGHTLVYMKDQKRTWNDKLYFYPIPANDLVMNPNLG
                     QNKGWD"
BASE COUNT          329 a          226 c          233 g          288 t
ORIGIN      
        1 ttcatatcga aaactcttta tcagcaagac tccaatttca agtgaggtac tctatgcagt
       61 tacattcgat aatacgatgg gggtggtaca ttccagcaac cgtcagtgga catcggtaac
      121 ctttggttct tgtccgagtt tggttcgtcc gtttgtaaat acctatctca tgcttgacgg
      181 tacaccattt acaaatcaga ccgattacgc aacaaaatca tttccccagg agtgtgtgaa
      241 tcgcgacagc cgacttgcac aaacggttcg tacacctggt tttacaagga ttaccggagg
      301 aaccactgtt aagacagctc ctaactatgc ttacgcaata accggttacc agacctgcaa
      361 atttacgttg gacgacacac agttcgataa tgtagacatt tgcgacaaca atgtgatatt
      421 attccgctat gcagaagttc tattaaatta tgccgaagca aaggccgaat taggatcgct
      481 tacagacgcc gattgggcta aaacagttgg tgcattacgt gcccgcgcag gtatcacagg
      541 cggaatttca agcaagcctg tagctgtaga tccctatttg aaaagcacct tcttcccaga
      601 cgtttcaaat ccggaaatac ttgaaatccg tagagaacga ggtatagaat tatcttttga
      661 aggattcgaa tggtcggatg tatgccggtg gaaattaggg aattcactta cccgcgattg
      721 ggaaggtatc tatataccgg aatttgaagt accttacgac atgaatggcg atggcacatt
      781 agatgtttgc tttaccaaga gtctgactcc tgcaaacaaa gttcccggcg tatattattt
      841 gtatgttgga gaaaagcttg ccaacggagc aaagaataac tcccagatag cagctgacgg
      901 acatactttg gtgtatatga aagatcagaa acgcacctgg aatgataaat tatactttta
      961 tccgattcca gccaatgatt tggttatgaa tccaaatctt ggacaaaata aaggttggga
     1021 ttaaacgcat aaaagattta ctccaccccc attccggaga taaaggtatc gaagaa
//
LOCUS       DAOQXQ010000664         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_186834_length_1074_cov_2.040236, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000664 DAOQXQ010000000
VERSION     DAOQXQ010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186834_length_1074_cov_2.040236"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKH81_09455"
     CDS             complement(<1..541)
                     /locus_tag="PKH81_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNQ98302.1"
                     /translation="MTPSRRRAPARGRLQELIKTARDEGWAQWIRSEADEKAVLKGCF
                     FDVQAGERVCTFFEKFLRHSKGQWAGQPFVPLPWQREDILFPLFGWKQRNGFRRFRVA
                     YIEVPKKNGKSTLCAGMGLYLLTKDDEPGAEVYSAAADIDQASIVYREAETMAQSSPA
                     LAKRLRILPSRKNILYPAMN"
     gene            complement(528..>1074)
                     /locus_tag="PKH81_09460"
     CDS             complement(528..>1074)
                     /locus_tag="PKH81_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HNQ98303.1"
                     /translation="TSVQIREIRTRGHTRADRSSFWEGISMGSPGRPPKPSIVKALEN
                     NPGKRPITVEVRYREGLSAKPPTWLSREGKKLWKHLVPQLLEIPGLLQLVDRTAMETL
                     CESYAIWKQAVQTIREEGTTYTGVTKGGYEYITERPEVKIAAKHQKLMMEALAVFGFT
                     PASRTRISIPQPAGDDGFDAL"
BASE COUNT          183 a          360 c          286 g          245 t
ORIGIN      
        1 tgttcattgc cgggtaaagg atgttcttcc tcgacggcaa gatcctcagc cgcttagcca
       61 gcgccgggga actctgggcc attgtctccg cttccctgta gacgatgctg gcctggtcga
      121 tgtcggcagc tgccgaatac acttccgcgc cgggctcgtc atccttcgtc agaagataga
      181 gccccatgcc tgcgcaaagc gtcgacttgc cgtttttctt gggcacctct atgtaggcga
      241 cccggaaccg acggaagccg ttccgctgct tccacccgaa aagagggaac aggatatcct
      301 cccgctgcca ggggagcgga acaaacggct gccccgccca ctgtcccttc gagtggcgca
      361 ggaacttctc aaagaacgtg cagacccgtt cccccgcctg gacatcaaaa aagcagccct
      421 tcagaactgc cttctcgtct gcctctgacc ttatccattg tgcccatccc tcgtcccgcg
      481 ccgtcttgat aagctcttgc agtcttcccc gggcaggtgc ccgccgccta gagggcgtca
      541 aagccatcat ctccggctgg ctgcggtatg gagatccgcg tccggcttgc cggcgtgaag
      601 ccgaaaaccg ccagggcctc catcatgagc ttctggtgct tcgccgcgat cttgacctcc
      661 ggccgctccg taatgtattc gtagccgccc ttggtgactc cggtataggt tgtaccctct
      721 tcccgaatcg tctggacggc ctgcttccag atggcatagc tctcgcagag ggtttccatt
      781 gcggttctgt ccaccagctg cagcaggccc ggtatctcca gcagctgagg caccaggtgc
      841 ttccaaagtt tcttcccttc cctggagagc catgtcggcg gcttcgcgga taacccctcg
      901 cgataccgga cctctacggt aatcgggcgt tttcccgggt tgttctcaag agccttcact
      961 attgacggtt tgggcggcct ccctggactg cccatgctga tcccctccca aaaactactt
     1021 ctatccgcgc gcgtgtgacc cctcgtccgg atttcgcgga tttggacaga tgtc
//
LOCUS       DAOQXQ010000665         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_187404_length_1072_cov_2.528024, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000665 DAOQXQ010000000
VERSION     DAOQXQ010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187404_length_1072_cov_2.528024"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKH81_09465"
     CDS             <1..575
                     /locus_tag="PKH81_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ98304.1"
                     /translation="ISEKEAIEKIMLTLSDRDVIVSTTGMASRELYELREKHGMGHER
                     DFLTVGSMGHASQIALSIALNQKTRRVVCLDGDGATLMHMGALATVGTRKPGNMIHVV
                     LNNAAHDSVGGQPTVARDIDLSSIAQACGYRKVQTVQDGESLTKALGQSAEGPLFLEV
                     IVTKGSRKDLGRPKASPQENKQALMKFLQD"
     gene            587..>1072
                     /locus_tag="PKH81_09470"
     CDS             587..>1072
                     /locus_tag="PKH81_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ98305.1"
                     /translation="MIRQAVIMAGGLGSRLKGRTESMPKGFLEIGGQAIVERSVQKLF
                     AHGVEEIIIGTGHCSEWYEKLAAKWKCIRLARNDRYADTGSMGTLACCAPLVKGDFLL
                     LESDLIYDDIGLFSLINDDHVNVILASGATRSGDEVYLESDEQGTLVKHSKNKQELSS
                     IF"
BASE COUNT          292 a          228 c          283 g          269 t
ORIGIN      
        1 aaatttctga aaaagaagcc atagaaaaga ttatgctgac gctatcggat cgggatgtga
       61 ttgtctccac caccggaatg gcttcacgcg agttatatga actgagggaa aagcatggaa
      121 tgggacacga acgagatttt cttaccgttg ggtccatggg ccatgcgtct caaatagccc
      181 tatcgatcgc ccttaatcaa aagacgcgcc gtgtggtctg ccttgatggc gatggcgcaa
      241 ccctcatgca catgggcgcc ctggcaacgg taggaacgag aaagccggga aatatgatcc
      301 atgtcgtatt aaacaatgca gctcatgact ctgttggagg acaaccgact gttgctcgcg
      361 atatcgatct ttcctcgatc gctcaggcct gtgggtatcg gaaggttcaa accgtgcagg
      421 atggagaatc gttaacaaag gctcttggtc aaagtgctga aggccccttg tttctagaag
      481 taatcgttac gaaaggatca agaaaagatt taggacggcc aaaggcaagt ccgcaggaaa
      541 ataagcaagc cttgatgaaa tttttacagg actaaggggt acgacaatga tacgacaagc
      601 ggtaattatg gcgggaggtc ttggctcccg tttaaagggc aggaccgagt caatgcccaa
      661 gggttttttg gaaattggcg gacaggctat cgttgagcga tcagtacaaa agctttttgc
      721 gcatggggtt gaggaaatca tcatcggtac cggccactgt agcgaatggt atgagaaact
      781 cgccgctaaa tggaaatgca tccgtcttgc ccgtaatgac cggtatgccg acaccggaag
      841 catgggtacc cttgcctgtt gtgccccgct cgttaagggg gattttttac tgcttgagtc
      901 tgatttaatc tatgatgata tcggtttatt ctctttaatt aatgacgatc acgtcaatgt
      961 cattctcgcc tcaggagcta ctcgttcggg agatgaggtg tacctggaat ctgatgagca
     1021 aggtactctc gttaagcatt caaaaaacaa acaagagctt tcttctatat tt
//
LOCUS       DAOQXQ010000666         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_188453_length_1069_cov_1.661736, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000666 DAOQXQ010000000
VERSION     DAOQXQ010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188453_length_1069_cov_1.661736"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKH81_09475"
     CDS             complement(<1..416)
                     /locus_tag="PKH81_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNQ98306.1"
                     /translation="MPGKSGNDQKAFYRYDLFISYASEDFEQARKLVTRLQQDGYRVW
                     FDKTEMPGTGQVSGNLGQAVDDSRQFIICLSDTYCRKDYTQYELQIIFSKDPANSRNT
                     RIVTQIAASLPEKIPDSISFVPRLDLSRQENYEQEY"
     gene            complement(830..>1069)
                     /locus_tag="PKH81_09480"
     CDS             complement(830..>1069)
                     /locus_tag="PKH81_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98307.1"
                     /translation="PGKHPQTEQVPDTSHQGSYHHGGQSLSSPLQQLAISGWIEWGVH
                     IPGQKEVDVQVPYRAMDYLLERREMLGKMETYFIL"
BASE COUNT          282 a          261 c          237 g          289 t
ORIGIN      
        1 tgatattcct gttcgtaatt ctcttgccga ctgaggtcaa ggcgtggaac gaagctgata
       61 ctatccggaa ttttttcggg aagagacgct gcaatctggg taactattcg cgtgttacgc
      121 gaattggcag gatccttgct gaagataatt tgcagttcat attgcgtgta atctttacgg
      181 cagtaggtat cactcaaaca aatgataaat tgccgggagt catccaccgc ctggcccaaa
      241 tttcctgata cctgaccagt tcccggcatc tcggttttat cgaaccagac acggtatcca
      301 tcttgctgca gtctggtcac cagtttgcgt gcctgctcga aatcctcgct ggcataactg
      361 atgaataggt catatctgta aaaggccttc tgatcattcc cactcttgcc gggcaaggat
      421 acctccttac aaggttgaat ctttcctgat cattgccttt ttaatgaagt actgaaacta
      481 taactgagcg aggctggata aaccccactc gccggataac tataccgcat gtcccgcgaa
      541 acaaaccgtt ttctaccgac tcatcgtgcc tgttaaacta taaccgacat tgttacaatt
      601 aagcaagagc gatgagtatc cactgcaatc gaagattagt acaaaaacgc gcataaataa
      661 cgaggcggga atttttgcct attcaggagc taggaggttg ccggacttaa taggctaatg
      721 ctgaaattgg cttccccatg actagccttg gaccaagagc tatcatacag gctaatatag
      781 taattatgcg aagagaaaat caagcaaatt caacactacc ctctcttttt tacaggataa
      841 aataagtttc catttttccc aacatttccc ggcgttctaa caagtagtcc atcgccctgt
      901 aagggacctg gacatcaacc tccttctgac cagggatgtg gacgccccac tcgatccacc
      961 ctgaaatggc cagttgctgg agggggctgg acaacgactg gccaccatgg tgataactgc
     1021 cttgatgact ggtatctggg acttgctctg tttgcgggtg ttttccagg
//
LOCUS       DAOQXQ010000667         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_188717_length_1068_cov_2.023692, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000667 DAOQXQ010000000
VERSION     DAOQXQ010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188717_length_1068_cov_2.023692"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..864
                     /locus_tag="PKH81_09485"
     CDS             283..864
                     /locus_tag="PKH81_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HNQ98308.1"
                     /translation="MTTKETADALGVSESTIQRHAKELGLTENGKQTELDERAVTIIK
                     TKIERSGRTDLRNVAELPNISTDLEMMALDAKVSAWKTRKIEELQKQLADAQPKIAFH
                     DAVTGSKDTIDIGEAAKVLAVKGYGRNNLFERLRNEGILMQNNQPYQKYIDAGYFRTI
                     ESSFTAPDGTTHVNIKTVVYQRGLDFIRKVITK"
     gene            861..>1068
                     /locus_tag="PKH81_09490"
     CDS             861..>1068
                     /locus_tag="PKH81_09490"
                     /inference="COORDINATES: protein motif:HMM:NF016381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HNQ98309.1"
                     /translation="MIEKPNYTQIPNVYFDSIMQKLNGSENLVFLAIMRKTFGWRKKK
                     DRISYSQIMEMTGLVKSTTAAALKS"
BASE COUNT          362 a          226 c          257 g          223 t
ORIGIN      
        1 tttctttctg actggaaaga gtcacaaggg gcaaggcttg agaatgttaa agcaagaaag
       61 cggtattatt cgattgatga tattccgtca gattgacatt agcacataac cgcgctatac
      121 tctccttagt caatcggttc tgccagatcg gttgacgacc gtcctgttcg gggcgataat
      181 actagaaacc tttattcgag cagggattag atgcgaacac atcgacccgc aaggcctggc
      241 agccgctcga gtaaaggttt ttttattttc cagaggtgga aaatgacgac aaaagaaacg
      301 gctgacgcgc tcggagtaag cgaaagcaca attcagagac acgcaaaaga gttgggctta
      361 accgagaacg gaaagcagac ggagttggat gaacgcgctg taacaatcat caaaacaaag
      421 atagaaagaa gcggacggac agacctccgc aacgttgcgg agctaccaaa catatctaca
      481 gaccttgaaa tgatggcact cgacgcgaag gtatctgcat ggaaaaccag aaagattgag
      541 gaattacaga aacagcttgc cgacgctcaa ccgaagatcg cctttcacga tgcagtgacg
      601 ggatcgaaag acacgataga catcggcgag gccgcgaagg ttctcgcggt aaaaggatac
      661 ggcagaaaca acctattcga gcgattgcgc aatgaaggca tactcatgca gaataatcag
      721 ccatatcaga agtacatcga cgctggatat ttccgcacga ttgaatcaag tttcacagcc
      781 ccggatggaa cgacacacgt aaatataaaa acggttgtat accagcgagg gcttgatttt
      841 atcaggaagg tgataacgaa atgattgaaa aaccgaatta cacacaaata ccaaacgttt
      901 attttgatag cataatgcag aaacttaacg ggtctgaaaa cctcgttttc ttggccatta
      961 tgagaaaaac gttcggatgg agaaagaaaa aagacagaat atcatactcc cagatcatgg
     1021 aaatgaccgg tcttgtaaaa tcaacaaccg cagcggcgtt aaaatctc
//
LOCUS       DAOQXQ010000668         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_189139_length_1066_cov_4.241345, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000668 DAOQXQ010000000
VERSION     DAOQXQ010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189139_length_1066_cov_4.241345"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..782)
                     /locus_tag="PKH81_09495"
     CDS             complement(9..782)
                     /locus_tag="PKH81_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNQ98310.1"
                     /translation="MAQYSVISDMDGVIYRGKALIPGAKEFVSRLVTSGTKFLFLTNN
                     SEQTPIDLVRKLNILGIDGLTEENFITSAMATAIFLATQKPAGSVYVVGGAGLSTELY
                     QRGFSITEHNPDYVVVGKTSSLSFDMISKAASLIRGGAKFIGTNPDMIDPVEKGVEPA
                     AGVILKAVEAASGKKPYVIGKPNSLMMMIARKKLMVHSEDTVMIGDRMDTDIVGGLEA
                     GMKTCLVLSGVSTRESIRDFPYRPDYIFDSVADINPELW"
     gene            complement(818..>1066)
                     /locus_tag="PKH81_09500"
     CDS             complement(818..>1066)
                     /locus_tag="PKH81_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ98311.1"
                     /translation="LIRALPRTGRTHQIRVHLSNSGCPILGDSLYGGPTTLPSLPALT
                     IPRILLHALSIQFVHPLNGQPMNLNTPIPPDFLPLLHE"
BASE COUNT          285 a          232 c          271 g          278 t
ORIGIN      
        1 tatttccatt accatagctc cgggttgata tctgcaacag agtcaaaaat ataatcaggg
       61 cggtaaggaa aatcgcgtat cgattcacgg gtggaaaccc ccgaaaggac caagcaggtt
      121 ttcatgcccg cttctaaacc gccaacaata tcggtatcca tacgatcacc gatcataacg
      181 gtatcttccg agtgaaccat cagttttttt ctggcaatca tcatcatcag cgaatttggc
      241 ttacctatga cgtatggttt ttttcccgaa gccgcctcaa cagcttttag gatgacacca
      301 gctgcaggct caactccttt ttctacggga tcgatcatat ctggattggt tccaatgaat
      361 ttagccccgc cgcggattaa agatgcggcc ttgctgatca tatcgaagct gagggaactg
      421 gtttttccca cgacaacata atcggggttg tgttcggtaa tgctaaaacc acgttgatac
      481 aactcggtgg aaagaccggc cccgcccact acgtatacgc tccctgctgg tttttgggtt
      541 gccaggaaga ttgcggtggc cattgcagag gtaataaaat tttcctctgt aagaccgtca
      601 ataccaagaa tgttaagctt gcgtacaaga tctattgggg tttgctccga gttattggtt
      661 aaaaaaagaa attttgtacc actggtaaca aggcggctaa caaattcctt agcgccagga
      721 attaaggctt tgcctctata gataacaccg tccatatctg aaattacgct gtattgcgcc
      781 atgagtacct ctctggtgga agtataccgg gactgaacta ctcgtgcaat aaagggagaa
      841 agtcgggtgg tattggggta ttcagattca tgggctgtcc gttaaggggg tgcacaaact
      901 gaattgatag ggcatgaagc aaaatgcgag gaatggttag tgccggaaga gaaggaaggg
      961 tcgtggggcc tccatagagt gaatctccca ggattggaca tccggagttg gaaagatgta
     1021 cacgaatttg gtgggttcgc cctgttcggg gcaaggctct gatgag
//
LOCUS       DAOQXQ010000669         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_189451_length_1065_cov_3.673267, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000669 DAOQXQ010000000
VERSION     DAOQXQ010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189451_length_1065_cov_3.673267"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1065)
                     /locus_tag="PKH81_09505"
     CDS             complement(<1..>1065)
                     /locus_tag="PKH81_09505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNQ98312.1"
                     /translation="MTRGLVKAAAASKDLTVGKEVQKMFIPLETDDTGRKLTCGSTVD
                     DHAEFFGYYEGAKGVSGDYFDYIKLDDRHYAIIKCDVAGKGVPAALIMVEVATLFLDF
                     FKDWKYERNGYRLEIIVSKINDLIESRGFKGRFAAFTLCIFDSASGMAHFCNAGDNLV
                     HRYSASRGKMEVITLPESSAAGVFPSFMIDMKGGFQVVSTKLDKGDVLFLYTDGIEEA
                     KRLFRTPSLEVYTCAESGLELDAPHGNHSVGQDNEEIGPDRVNAIIEAVLSRKRYALQ
                     KWHNPEGDVTYEFDFSDCQGTIEEAILALVSVEKVFRMYLDGQATDFDRVQVDRRVDR
                     FLSAHFLQYERYCSQRRDHP"
BASE COUNT          315 a          278 c          203 g          269 t
ORIGIN      
        1 caggatgatc gcgacgttgg gaacaatatc gctcatactg caaaaaatga gcggatagaa
       61 atcgatctac cctgcggtca acctgaacac gatcaaaatc tgtggcctgc ccgtccagat
      121 acatccggaa cactttttcc accgaaacaa gcgctaaaat agcttcttct atagttccct
      181 gacaatcact aaaatcgaat tcataggtaa catctccttc aggattatgc catttttgca
      241 aggcatatcg ttttctggaa aggacggcct ctataatggc gttaacacga tcaggaccaa
      301 tctcctcgtt atcctggcca accgaatggt ttccatgtgg agcgtcgagt tcaagcccac
      361 tctctgcaca ggtatacacc tctagagatg gagttcggaa taaacgcttt gcctcttcaa
      421 taccgtcagt atacagaaac aaaacatcac ccttatccag tttagtgctc actacctgga
      481 aaccaccttt catatcgatc ataaaagaag ggaatacccc ggctgcggaa ctttccggga
      541 gcgtgattac ttccatcttt ccacgagatg cgctatagcg atggacaagg ttatctcccg
      601 cattgcagaa atgagccatt ccggatgctg aatcaaaaat acacaaggta aaggcggcaa
      661 agcgcccctt aaatccgcga gattctataa gatcatttat ctttgaaacg ataatctcca
      721 atcgatatcc attgcgttca tacttccagt ccttaaagaa atccaaaaac agggttgcaa
      781 cctcgaccat gatcaaggct gcgggaacac ctttaccagc gacgtcgcat ttaattatgg
      841 cgtaatgacg atcgtccagt ttgatataat caaaatagtc tccagaaaca cccttggccc
      901 cctcgtaata cccaaaaaac tcggcatggt cgtcaacggt gcttccgcag gtaagctttc
      961 ttccggtatc atcagtttcc aatggaataa acatcttctg aacttcttta ccaacagtca
     1021 aatccttgga tgccgctgct gccttaacaa gaccacgagt catat
//
LOCUS       DAOQXQ010000670         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_189882_length_1064_cov_2.271556, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000670 DAOQXQ010000000
VERSION     DAOQXQ010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189882_length_1064_cov_2.271556"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1064)
                     /locus_tag="PKH81_09510"
     CDS             complement(<1..>1064)
                     /locus_tag="PKH81_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98313.1"
                     /translation="PRWFLEQSLASMDHHGLDWTLADTRLTMYRLGWLGQENPSRDFH
                     QAGYPAIWIQGNQNIIPALEHMARSLAIDHPTQKDSHYLLRYLRGRLVFIGEEVTVIL
                     MIISFTLILSFVFIFSFLHGKQNEQRLRDLAKLWWLPFTYLVVTTLCLIAGQAVAQLI
                     VRSRFSDSSAWALLPRTALMTKFAFAWFFITIIFSLNQVIHFPSDSFVYGYIASIAAM
                     INIFVFSAMDFSLSILFLTVYLISIAAYRISPPVFHLPLIALMALPFFPYAKTLLLSG
                     KPAIESIFLADTTANLRIAFFLIPFQLLFARFFHRLGLYGRRQRFYLPVNLFVSSIPL
                     LLVSGFSLFYPAWSPAKPLE"
BASE COUNT          355 a          214 c          281 g          214 t
ORIGIN      
        1 cctcaagcgg ttttgcaggc gaccatgcgg gataaaaaag ggagaaaccg gaaacaagaa
       61 ggagcggtat actggataca aacaaattga ccggaagata aaaacgttgt cgccgtccat
      121 acaggccaag acggtgaaaa aatcgcgcaa acagtagctg aaacggaatt agaaaaaatg
      181 cgatgcggag gttcgctgtg gtatccgcaa ggaatattga ctcaatagcc ggcttgccgc
      241 ttaacagcag ggttttagcg taaggaaaga aaggaagcgc catgagcgcg atcaggggga
      301 gatgaaaaac aggtggagag attcggtagg cggcaatgct gattaaatac acggtaagaa
      361 aaaggataga aagcgaaaaa tccatcgcag agaacacaaa gatatttatc attgccgcga
      421 tgcttgcaat gtagccgtag acaaagctgt ccgatggaaa gtgaataacc tgattgagtg
      481 aaaaaataat cgtaataaaa aaccaggcaa aggcaaattt cgtcatcagg gcagttcggg
      541 gaagtaaggc ccacgccgaa gaatccgaaa atctggatct aacaattaac tgtgcaacag
      601 cttgtccggc tataaggcat agagtcgtaa caaccaggta ggtaaaggga agccaccaaa
      661 gctttgccag gtctcgcaga cgctgttcat tttgcttgcc atgtaaaaaa ctaaaaataa
      721 atacaaagga gagaattaag gtaaacgaaa taatcatcag gattacggta acttcttctc
      781 caataaaaac taggcgtccc ctcaggtatc gcaaaagata atggctgtct ttttgtgtag
      841 ggtggtctat cgcaagagac cgggccatgt gctccagggc cgggatgatg ttttgatttc
      901 cctgtatcca gattgcagga taccccgcct ggtggaaatc ccgggaggga ttttcctgcc
      961 caagccaacc cagtcgatac atggttaacc gagtgtcggc aagggtccag tccagaccat
     1021 gatgatccat ggaagccagg gattgttcaa gaaaccatcg ggga
//
LOCUS       DAOQXQ010000671         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_191048_length_1060_cov_2.453731, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000671 DAOQXQ010000000
VERSION     DAOQXQ010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191048_length_1060_cov_2.453731"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKH81_09515"
     CDS             <1..569
                     /locus_tag="PKH81_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98314.1"
                     /translation="LLAHGLQLVADDRSDARALSFPDTKIRLVTISSPFNGIKASSHC
                     GIPALHIASVGTTVVICQAIAGSTWSEIHPSSDFINNPGQMSVNVSTHLKINTDEDNI
                     CRTQDAQGNCMEDDFVFGLDEQYSSVIDNDTRVSNIELKAGHARVIGSSGRPPFELIE
                     TLESTSIMKKQTTLSYAENLALLERLYQ"
     gene            589..>1060
                     /locus_tag="PKH81_09520"
     CDS             589..>1060
                     /locus_tag="PKH81_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ98315.1"
                     /translation="MSSMRRHQLSLSVLAFVLCVSCTQTPIDTAIKGITRGDNATLRI
                     EWQGKVFYVDPIGTIPSERVDAVFISHRHSDHYDPVALSTFNPTVPIYAPFDAKNITR
                     LPVGQKIVVAGIEIEAVPSYNIEKKQYHPQKDGNAGYIFTLDHIRLYVAGDTERI"
BASE COUNT          279 a          263 c          223 g          295 t
ORIGIN      
        1 aactgctcgc gcacggcctt cagctcgtcg ccgatgatcg cagtgatgcg cgcgctcttt
       61 cattcccgga tacaaagatt cgcctggtta ccatttccag tccatttaac ggcatcaagg
      121 cttcttctca ttgcgggatt cctgccttgc acattgctag tgttggcact actgtcgtca
      181 tctgtcaggc aattgccggg tctacctgga gcgagattca tccttcctct gattttatta
      241 ataaccccgg ccagatgtct gtaaacgtat ctacacatct caaaatcaat actgatgaag
      301 ataacatctg ccgcacccaa gatgcccagg ggaattgtat ggaagacgac tttgtgttcg
      361 gtttggatga gcagtattct tccgttatag acaatgatac acgagtcagt aatattgaac
      421 ttaaggcagg acatgctcgt gtaattggtt cttcgggacg ccccccattt gagcttatag
      481 aaacgcttga atccacctcg atcatgaaaa agcaaactac tctcagttat gcggaaaacc
      541 tcgcactact ggagcgatta taccaataaa ttattacgag gttttataat gagtagtatg
      601 cgtagacatc agctttccct ttctgttctt gcctttgtat tgtgtgtatc ctgtacacag
      661 acacccatcg ataccgctat taagggcatt acccgtggag acaatgcaac gctcaggatt
      721 gagtggcaag gtaaggtgtt ctatgttgac ccgatcggca ctattccaag tgaacgagta
      781 gacgctgtct ttattagcca ccgacacagc gatcactatg atccggtcgc cctttccacc
      841 ttcaacccaa cggtaccaat atatgcccct tttgatgcaa aaaacattac ccgtcttccc
      901 gttggacaga aaattgtagt tgcgggtata gaaatagagg cggttccttc ttacaatatc
      961 gaaaagaaac aataccatcc acaaaaagac ggtaatgcag gttatatttt cactctggac
     1021 catattcgcc tctatgttgc cggagacacc gagcgtattc
//
LOCUS       DAOQXQ010000672         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_191287_length_1059_cov_3.318725, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000672 DAOQXQ010000000
VERSION     DAOQXQ010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191287_length_1059_cov_3.318725"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKH81_09525"
     CDS             complement(<1..261)
                     /locus_tag="PKH81_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNQ98316.1"
                     /translation="MRPLIEDREAVLARLGGSETMLARLLDKFCRSYSRAPEELETLL
                     ESGQHEEARRLVHSVKGVSANLGLASIYRSSIALENKLKDESE"
     gene            362..>1059
                     /locus_tag="PKH81_09530"
     CDS             362..>1059
                     /locus_tag="PKH81_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ98317.1"
                     /translation="MTIKNARNFVCILTFAGLIFLSCVSNPTTPQPSPADGNSLATLV
                     ASGQSAEVKKRFTNAESVNQKDAQGQTLLHVAALQNNADMVQILLDLKADTRIKNNSG
                     ETAMAAAVNAGCYDAVKTLALAGSDIFATNAAGMSVYNQARQRGPDAFSAILSPATIV
                     LQDPDGKTALHHACASLDEDAVSAILAVGSPSSRTDSTGETPLAASYKNPVSDASARI
                     ASLILLAAGEPLRG"
BASE COUNT          273 a          307 c          242 g          237 t
ORIGIN      
        1 ctctgactca tcttttaact tgttttccaa agcgattgag cttcgataga tgctcgccag
       61 tcccagattg gcggaaactc ccttaactga atgaacaaga cgtcttgcct cttcgtgttg
      121 tcccgactcg agtaacgttt ccagttcctc gggagcccgc gaatacgagc ggcagaattt
      181 atccaaaagc cgggcaagca tcgtctcgct tcccccaagc cgggccaaaa cagcctctcg
      241 atcctctatt aagggtctca ttgcatccat ccctgttatt gtataccgat ccgcacgatt
      301 ttacagcaaa atcgcatcaa ccaagacact tttccggtcg atgaatatag gggagagtat
      361 catgaccatt aaaaacgcac gcaatttcgt atgtatcctt actttcgcag gccttatatt
      421 cctgtcctgc gtatcaaatc caactacacc gcaaccgtca cctgcagatg gaaatagtct
      481 ggcaacgctg gttgcaagcg gccagagtgc cgaggttaaa aaacgcttta ccaacgcaga
      541 atcggtcaac caaaaagacg cacaaggaca aacactcttg catgttgctg cactgcaaaa
      601 caacgcagac atggttcaaa ttcttttgga ccttaaggcc gacacccgca ttaaaaacaa
      661 ctcaggagaa acggcaatgg ccgcagcggt taatgccgga tgctatgacg cagtaaaaac
      721 ccttgccctg gccgggtcgg atatctttgc taccaatgcc gcgggaatgt ctgtgtataa
      781 tcaagcgcga cagagaggcc ccgacgcatt ttcggccatc ctttcgccgg cgaccattgt
      841 gcttcaggac cccgatggca aaacagccct tcatcatgcc tgcgcgtccc tggatgaaga
      901 cgcggtaagc gccatattgg cagtaggatc gccctcatcg cgcaccgaca gcaccggaga
      961 gacacccctg gctgcctcgt ataaaaaccc ggttagcgac gcaagcgctc gtatcgcatc
     1021 tctgattctt cttgccgcag gggaaccctt gcgaggcga
//
LOCUS       DAOQXQ010000673         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_193898_length_1051_cov_3.201807, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000673 DAOQXQ010000000
VERSION     DAOQXQ010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193898_length_1051_cov_3.201807"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..804
                     /locus_tag="PKH81_09535"
     CDS             70..804
                     /locus_tag="PKH81_09535"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HNQ98318.1"
                     /translation="MTDFKNSGFMFAIGGCVALFVIAQSVFFLRKAIERGRELGISSE
                     TVRKTIRSSAIFSVLPSVSILLGLITLSYALGLPLPWIRLSVLGAVTYELPAATAAMN
                     ALGSSITEPISSAHALSAVAWVMTIGCISPLIIIPLFLKRIQRGVLSIQQMDNRWGAI
                     FMSALFLGMISAFLGMGISGGLLSILVLLSSAAIMMACGILIKKCGLFWLEDFALPTS
                     MLASMALAVLYARLLPESVLPINLFK"
     gene            815..>1051
                     /locus_tag="PKH81_09540"
     CDS             815..>1051
                     /locus_tag="PKH81_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98319.1"
                     /translation="MKDTHATVLTGYERNIHRWGRIWMMCALAVMLAVPIAMCLHFNS
                     WPGLIPVIKGLLAVAPVYWTVCAIEVLTFAPMLGA"
BASE COUNT          210 a          242 c          261 g          338 t
ORIGIN      
        1 tggcttactt ttttatggga aaaggaggaa aataccaaca atcctgcgag cagtatatta
       61 ggagccgata tgacagattt taagaacagc ggatttatgt tcgctatcgg cggatgcgtg
      121 gcgctatttg tgatcgcgca atccgttttc tttttacgaa aggccataga gcgaggtcgc
      181 gagttaggaa ttagctctga aacagtccgt aagactatcc gctcctcggc aatattttct
      241 gtgttgcctt cggtgtctat tttactcggg ctaattacac tctcgtatgc cctgggttta
      301 ccccttccct ggattcgctt aagcgtgttg ggggcggtaa cgtatgagct tcccgcggcc
      361 actgcggcca tgaacgccct gggctcatcc attaccgaac ccatatcaag cgcacacgca
      421 ctgtccgcgg ttgcctgggt catgacgatt ggctgcatct ctccgctcat tattattcct
      481 ctttttctta aacgaattca gcgtggtgtt ctatccattc aacaaatgga taaccgatgg
      541 ggtgcaattt ttatgtctgc tctctttttg ggaatgatct cggcctttct tggtatgggt
      601 atctccggag gtcttctctc tatattggtc ttgttaagtt ctgcggcgat tatgatggcg
      661 tgcggaatac tgataaaaaa atgtggtctt ttctggcttg aggattttgc cttacctacg
      721 agcatgcttg cttccatggc gcttgcggtt ctctatgcgc gtcttttgcc cgaaagtgtg
      781 cttccgatta atttgtttaa ataaggggaa tgctatgaag gatactcatg ctactgtatt
      841 gactggttac gagcggaaca ttcaccgatg gggaagaatc tggatgatgt gcgcccttgc
      901 tgttatgctg gctgttccta ttgcgatgtg tcttcacttt aattcttggc ccgggcttat
      961 tcccgtaatc aaaggacttc ttgccgttgc tccggtgtac tggactgtat gcgccattga
     1021 agtgctcacc tttgctccca tgcttggcgc t
//
LOCUS       DAOQXQ010000674         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_195240_length_1047_cov_2.877016, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000674 DAOQXQ010000000
VERSION     DAOQXQ010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195240_length_1047_cov_2.877016"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKH81_09545"
     CDS             complement(<1..291)
                     /locus_tag="PKH81_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ98320.1"
                     /translation="MKIGIDAFGCDHGRSGVGSYILSLVRNLPQNDAIIDLFGPELDR
                     YTYTSGQEKASYSGLSVSDSLLAERVWHLLHYPRFVTKQKYDAVLFPAGVRLL"
     gene            367..>1047
                     /locus_tag="PKH81_09550"
     CDS             367..>1047
                     /locus_tag="PKH81_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ98321.1"
                     /translation="MNTFTDLSLSTDLVEALGASGITKPTPIQCAALPRLLTGENLLF
                     SSETGTGKTLCYLLPAFEKCRQETERRAPAVLIIAPTHELASQIKRESLSLAERSHIP
                     VTTALLVGGAPLKRQIDVLKQKPRIVVGGPARIIELVRLKKLDIRSVSMLVLDEIDRM
                     LAPEMRDMVKELILMIPDSAQLIACSATISAYQARLVEDMARESGRVKAAPASYIVPL
                     LSQPSEDIL"
BASE COUNT          293 a          266 c          236 g          252 t
ORIGIN      
        1 aagaagacgt actcctgcgg ggaataacac agcatcatat ttttgcttag taacaaagcg
       61 gggatagtga agcaggtgcc atactcgttc ggcaagcaga ctatcggaaa ctgatagacc
      121 tgagtatgag gccttttcct gccccgaggt ataggtataa cgatccagtt caggaccaaa
      181 aaggtcaata atcgcatcgt tttgaggaag attccgtacc aatgacagta tatacgagcc
      241 gactccggag cggccatgat cgcaaccaaa ggcatcaata ccaattttca aaagtgtctt
      301 cctccaaagc tgtcgggtat aaaaccgatt atagcacttt tatctccaat atgctatcct
      361 gaacaagtga atacctttac cgacctttcc ctttccacgg accttgtaga agctcttgga
      421 gcatccggga ttaccaagcc cacaccgata cagtgtgcag ccctgccccg ccttcttaca
      481 ggcgagaatt tactctttag ctcagaaaca ggaacgggga aaaccctttg ctacctgcta
      541 ccagcctttg aaaaatgtcg gcaggaaacc gagcgacgcg ctccggcagt gttgattatt
      601 gccccaacac atgaacttgc ctcccaaatt aaacgagagt cattatctct tgcagaacgt
      661 tcacatatcc cggttaccac agcgctgctt gtgggtggag ccccgcttaa acgtcaaatt
      721 gatgtactca agcaaaagcc ccgtatcgtg gtaggaggtc ctgcccgtat tattgaactt
      781 gtacgtctta aaaaactgga tattcgatct gtgtccatgt tggtactgga cgagattgac
      841 cgcatgctcg ccccggaaat gagggatatg gttaaggaat tgatcctgat gattccagat
      901 tcagcccagc ttattgcctg ttcagcaaca ataagcgcat atcaagcacg actcgtagaa
      961 gatatggccc gcgaaagcgg gagggttaag gcggcaccag cctcctatat tgtcccactc
     1021 ttgtcccaac caagcgaaga tatatta
//
LOCUS       DAOQXQ010000675         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_195933_length_1045_cov_2.650505, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000675 DAOQXQ010000000
VERSION     DAOQXQ010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195933_length_1045_cov_2.650505"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKH81_09555"
     CDS             complement(<1..878)
                     /locus_tag="PKH81_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98322.1"
                     /translation="MKCKQATYVLVAAVLVLVLALSTRVYFGFTKESFHVDEGITLAL
                     TNGNWAPGMDVVTKDAWISGEVAKDMTFNATIAERGSPDIKAIAISTAHDVHPPLYYW
                     LFGYARTILGVHRSVTAGYVLNLALFMLSCVFFVVLVRRIWSDPVIIIAALACFALSS
                     AAVSLSVFLRMYELLQTVCLGLLLSAHCVLFPSVKAEQSVLSRSLEFWLGLVGLFACA
                     LAGFLTQYYFLFFVAPVCAFALVWLLVQKRPGDLFWGIVAVLAAFYLADMIFPPMKAH
                     LTESYRAKQSIQNFASA"
BASE COUNT          333 a          272 c          214 g          226 t
ORIGIN      
        1 gcactggcaa aattttgaat actctgcttt gcgcgatagg actccgtcag atgtgccttc
       61 atcgggggga atatcatgtc tgcaagataa aaggccgcca acaccgccac gattccccaa
      121 aatagatcgc cgggtctttt ttgcacaagc aaccacacca aggcaaaagc gcatacaggc
      181 gctacaaaaa agagaaaata atactgagtt aaaaaaccgg ctaaagcgca ggcgaaaagg
      241 cctaccagtc ccaaccaaaa ctctaacgat cggctcaaga cactttgttc cgccttgaca
      301 gacgggaaca acacacagtg tgcactcagc aacaagccca aacatactgt ctgcaacaac
      361 tcatacatac gcaaaaacac agaaaggcta acagcagcag aggaaagggc aaaacaggca
      421 agggcggcaa tgataattac cggatcgctc caaatgcggc gcacaaggac aacaaagaaa
      481 acgcaagaga gcataaaaag tgccaggtta agcacatatc ctgctgtaac cgaacggtgc
      541 acgcccaata tggttcgtgc atacccaaac aaccagtaat acaagggtgg atgcacatca
      601 tgtgccgtgg agattgcaat agcctttata tccgggcttc cccgttcggc tatggttgca
      661 ttaaaggtca tatcctttgc cacttccccg ctgatccacg catccttggt aactacatcc
      721 atacccggag cccagttacc attggttaat gcaagggtaa ttccttcatc gacatgaaag
      781 gattctttgg taaacccaaa gtacacccgt gtcgaaaggg ccaaaaccag gaccaatact
      841 gccgcaacaa gaacatacgt tgcctgttta cacttcatcc attcttctcc ttacgctttt
      901 tatctcgctt ctagtttacg aacgttcctt aatgatatag acaggacgct tcttggtttc
      961 cagatatgtt tttgcaagat actgcccaag gataccaata gtaaaaagct ggctgaaaac
     1021 cggattccat aagaattttt ctacc
//
LOCUS       DAOQXQ010000676         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_196058_length_1045_cov_1.963636, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000676 DAOQXQ010000000
VERSION     DAOQXQ010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196058_length_1045_cov_1.963636"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKH81_09560"
     CDS             complement(<1..597)
                     /locus_tag="PKH81_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNQ98323.1"
                     /translation="MGLALFVAAFPVLFMNEGCAVKTYRDLSSLGNEVISVDSLKVDP
                     ANNLKPVHMSGTAKTDEVLTDSAFNYSANAIHLDRSVEMFQWIESKSQKTQKKLGGGE
                     ETVTTYDYKTGWSSSAVDSSIFKQPQGHQNPGAMPYEAMRISAQSVVLGAFRLIPGQI
                     SGIGGAETVNFTAAEIAKLPPDIRAKAKPSGEWLFLGAN"
BASE COUNT          242 a          273 c          289 g          241 t
ORIGIN      
        1 attggcgccc aggaagagcc attcgccgga gggcttggcc tttgcccgaa tatcgggcgg
       61 aagcttggcg atctcggccg cggtaaagtt gacggtctcc gcccctccta taccgcttat
      121 ctgtccgggt atgagcctga atgccccaag tacgacgctt tgggccgaga ttctcatggc
      181 ctcatagggc atggcgcccg ggttttggtg gccctggggc tgtttgaaga tcgaggaatc
      241 gaccgcggac gatgaccagc cagttttata atcgtaggtg gtgaccgtct cctctcctcc
      301 gccaagtttc ttctgggttt tctggctttt ggattcaatc cactggaaca tctcgaccga
      361 acggtccagg tggatcgcgt tggcgctgta attgaatgcg ctgtcggtta acacctcgtc
      421 ggtcttcgcg gtaccgctca tgtgtaccgg tttaaggttg ttggccggat ccacttttag
      481 agaatctacg cttataacct cattccccag gctgctcagg tcgcgatagg ttttaacggc
      541 gcaaccctcg ttcataaaga gtaccggaaa ggccgcgacg aagagggcga ggcccacaag
      601 cacccccttg atggactcca tgaggcgcgc gccccatgat ttggaactga cttccgtaac
      661 cgaatcggac atcgtatctc ctccctgatt ttgattctgt cggaaacggc cgacaaggcg
      721 tgagagtagt atgttaaaaa acaaatgtca accaataatc gggcaatatt acgctaagct
      781 gccattcttt aacaggacaa catggggtat gtatcataat acagtggact tgttgcgtat
      841 ctgacaaaaa tgtcgcgcgc gagaaggccg caggccgacg tggcgatctc agaatacaag
      901 gaattattca tagaaacaga aagctaaaga gattgcgctg gccaggatgg ccgagtgccg
      961 cgaagcacac ggatgtgcgt gagcggccca cgccccgata cgaccggggc tcgcaatgta
     1021 ctcagtaaag caacaagtct aatat
//
LOCUS       DAOQXQ010000677         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_198552_length_1037_cov_3.114053, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000677 DAOQXQ010000000
VERSION     DAOQXQ010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198552_length_1037_cov_3.114053"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="pdxS"
                     /locus_tag="PKH81_09565"
     CDS             complement(<1..786)
                     /gene="pdxS"
                     /locus_tag="PKH81_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNQ98324.1"
                     /translation="MDQKEQFAVKIGLAEMLKGGVIMDVTNAEQAKIAEQAGAAAVMA
                     LERIPSDIRRDGGVSRMSDPKMIAEIQKAVSIPVMAKCRIGHFVEAHILEALEIDFID
                     ESEVLTPADDEFHISKHQFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGNVVEA
                     VRHMRQVMADIRRIANMDEAELMTAAKELQAPFELVLETRKLGKLPVPNFAAGGVATP
                     ADAALMMHLGAETVFVGSGIFKSKDPEQRAKAIVRAVTHYRDQK"
     gene            complement(804..>1037)
                     /locus_tag="PKH81_09570"
     CDS             complement(804..>1037)
                     /locus_tag="PKH81_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98325.1"
                     /translation="PGASSLTLLPLAIDAGVSFLPGPLQDSEYRCINSLRLCYGSLSP
                     AEISEGIRRLAKVVRMYLTERHQDPIPAGIGDY"
BASE COUNT          234 a          268 c          255 g          280 t
ORIGIN      
        1 cttttggtcg cggtagtggg taacagctct aactatagcc tttgctcgct gctccgggtc
       61 tttagattta aatataccag aacccacgaa gacagtctct gcgccaaggt gcatcataag
      121 agcggcgtcg gccggtgttg ctactcctcc tgcggcgaaa ttgggaacag gaagttttcc
      181 gagcttgcga gtttccagaa ccagttcgaa gggagcctga agttctttgg ctgcggtcat
      241 gagttcggct tcgtccatgt tggcaatgcg gcgaatgtcg gccattacct gtcgcatgtg
      301 gcgtactgct tcaacaacgt tcccggtacc cgcttctccc tttgtacgga tcattgcagc
      361 tccttcccca atacgacgga gtgcttcgcc cagatttcgg catccacata caaagggaac
      421 cttaaattgg tgtttgctaa tatgaaactc gtcgtcagcg ggggtaagga cctcgctttc
      481 gtcaataaag tctatttcga gggcttccag aatgtgcgct tctacaaaat gtccgatacg
      541 gcattttgcc ataacgggaa tagaaacggc tttttgaatc tcggcgatca tcttgggatc
      601 gctcattcgt gatacaccac catctctgcg tatgtcagag ggaattctct ctaaggccat
      661 aaccgctgcg gctccagcct gttcggcgat ttttgcctgc tcggcgttgg ttacatccat
      721 tataaccccg cccttaagca tctctgccaa accaatttta acggcaaatt gttctttctg
      781 atccatcgta ctctcctttc ggtttaataa tctccgatac ccgcagggat cggatcctga
      841 tgtctttctg taagatacat acgcacgacc tttgcaaggc gtcgtatacc ttccgatatt
      901 tccgcagggc tcaacgagcc ataacagagt ctgagtgagt tgatacagcg atattctgaa
      961 tcctgcaaag ggccaggaag gaaagatact cctgcatcga ttgcgagcgg gagtaaggtt
     1021 agactggaag cgccggg
//
LOCUS       DAOQXQ010000678         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_199282_length_1035_cov_2.429592, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000678 DAOQXQ010000000
VERSION     DAOQXQ010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199282_length_1035_cov_2.429592"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKH81_09575"
     CDS             complement(<1..526)
                     /locus_tag="PKH81_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ98326.1"
                     /translation="MNEGAYLSVLNPEQREAVTHEGSPLLILAGAGSGKTRVITTKIA
                     WLIGERGVDSRSILAVTFTNKAAREMSERARRLESRAEESVLRTFHSFGAWILRRNAS
                     LAGLDPNFTIYDDDDATTLLSKALPALTRQQASLAVRKIARAKDYCLTPDDPLLVEIE
                     SDPDFAQVYRTYEKR"
     gene            complement(523..>1035)
                     /locus_tag="PKH81_09580"
     CDS             complement(523..>1035)
                     /locus_tag="PKH81_09580"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNQ98327.1"
                     /translation="GRSFLALRTTILTGFPGESDESFELTARFLADIRPLWSGSFTYS
                     PEEGTVAAELKKKVPKKTALARAAAFKELQTRITEEELAVFVGQELTVLVEEVIPDQG
                     SPDPADPVPPSRLALGRAWFQAPEVDGSVVLQFEDGQTGVDGESVCAGSVVKALVIAV
                     HGVDVEAVVQ"
BASE COUNT          241 a          293 c          270 g          231 t
ORIGIN      
        1 gacgcttttc gtacgtgcga tacacctggg caaaatcggg gtcgctttct atctcgacaa
       61 gaagtggatc gtcgggcgta aggcagtagt ccttagcccg cgcgatcttc cgtaccgcaa
      121 gggacgcttg ctggcgggta agggcgggca gggccttgga caagagagtt gtcgcgtcat
      181 catcgtcata aatagtaaag tttggatcaa gtccggcaag ggaagcgttt cgacgcaaaa
      241 tccaggcgcc aaacgaatgg aaggttctta gtacggattc ttcggcacga ctttcaagtc
      301 gtcgagcccg ctcgctcatc tccctggctg ccttgttggt aaaggtaacg gccaggatag
      361 acctgctgtc cactccccgc tcgccgataa gccaggcaat cttggttgtt attacccgag
      421 ttttccccga acctgcaccg gcaaggatca atagcggaga tccctcgtgg gtaaccgcct
      481 cccgttgttc cgggtttaat actgaaagat aggcaccttc attcattgca ccaccgcttc
      541 tacatccacg ccatgaacgg cgataactaa agccttaact acagagcctg cacaaacgct
      601 ctcgccgtct acaccagttt gaccatcctc aaactgaagg actactgagc catcaacctc
      661 tggagcctga aaccaggcgc gtccaagggc aaggcgggac ggaggaactg gatctgccgg
      721 gtcgggactg ccttgatcag gaatcacctc ttcaaccaat accgtaagct cctggccaac
      781 aaaaaccgca agttcttcct cggtaatccg ggtctgtaat tccttaaagg ctgcagctct
      841 ggctaaggcg gttttcttgg gaaccttctt ctttagttcc gcggctaccg tcccctcctc
      901 cggggaatag gtaaacgagc ctgaccacag ggggcgtatg tccgcaagaa accgtgcggt
      961 tagttcaaac gattcatcac tctctccggg aaatccggtt aatattgttg tacgcaaggc
     1021 caggaaggag cgccc
//
LOCUS       DAOQXQ010000679         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_200911_length_1030_cov_2.893333, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000679 DAOQXQ010000000
VERSION     DAOQXQ010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200911_length_1030_cov_2.893333"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKH81_09585"
     CDS             <1..785
                     /locus_tag="PKH81_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98328.1"
                     /translation="GPHIARQGIMERHYAQINKAYREGCPALSADAMEKLAEHFGEVP
                     QSCDVLGGESFMKFFGMKPSEMNDLWVSSQGFGKVKKLSPGVYCAPYSTGRFMTKYLL
                     NGFHAMQLANFTSPGRITAAVSLRSGDNASSWSEMRSDFAGATDPSRAKSGSVRNWLL
                     NNRAAIGMQTVDTQLNGIHLSAGPIESMAEIPNWFPWVKPHQTVMGRLLGAFMSEEEI
                     SGLALNPEVPGQGKPIFDLTEGKEPEDAVQAAYAVKSSRMEP"
BASE COUNT          277 a          237 c          283 g          233 t
ORIGIN      
        1 cgggtccgca tattgcccgg caggggataa tggagaggca ttacgcccag ataaacaagg
       61 cttacaggga aggctgcccg gccctttccg ccgatgccat ggaaaaactt gcggagcatt
      121 tcggtgaagt cccgcaaagc tgtgatgtgc tgggcggaga atcctttatg aagttttttg
      181 ggatgaagcc gagcgagatg aacgatctgt gggtaagctc ccagggtttt ggcaaggtca
      241 aaaaactatc tccgggggtt tattgcgctc cttacagcac cgggcgcttc atgaccaaat
      301 atcttttgaa cggattccat gctatgcagc tggccaattt tacttctccc ggccggatca
      361 cggccgcagt ttccctgaga agcggagaca atgcttcttc ttggtcagaa atgagaagcg
      421 actttgccgg ggctacggac ccttccagag cgaaatccgg aagcgtcagg aactggcttt
      481 tgaacaatag agcggctatc ggcatgcaaa cggtggatac tcaattaaac ggcatacacc
      541 tttccgccgg acctatagaa tctatggccg agatacccaa ctggttcccg tgggtaaaac
      601 cccatcagac agtaatgggc agactgctcg gggcattcat gtcggaagaa gagatatcgg
      661 gtcttgccct gaatccggaa gttcccggac aagggaagcc gatattcgac cttactgagg
      721 gcaaagagcc cgaagacgcc gttcaagcag cttatgccgt aaagagttcg cggatggagc
      781 cttaacgcgc cgcatttgat tattttacct ttatttgtta atatataccc tatcaatata
      841 acagggaagg ggtgttagga aaatggtagt agaaagatca gagtaccagg gcaagccgat
      901 gatcgtgctt aaaaggaacg agaacgacag gtatcctttt atgttcgggc tgtccaaggc
      961 caagcttgtt gtagaaaata tcgaggaagt aaagaaattc atagccgaga acgaccagcc
     1021 ggcttagatc
//
LOCUS       DAOQXQ010000680         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_200924_length_1030_cov_2.734359, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000680 DAOQXQ010000000
VERSION     DAOQXQ010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200924_length_1030_cov_2.734359"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKH81_09590"
     CDS             complement(<1..871)
                     /locus_tag="PKH81_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ98329.1"
                     /translation="METIHDLGSYTFTAMLANSLKKFSDRPALSYVSGTPINYAEVGT
                     RIETLRNMLWTMGIRKGDRVILFGPSSPNWGIAYFAIVTIGAIAVPLLPDFSVKEAET
                     CIRHSGASSMIVQSRLSEKVSNPSALGLDRVIRLDDLSLLSGTLGEVIPFPGAQIHEE
                     DTASIIYTSGTTGRSKGVELSHRNLVFTAIGGQFFQRINKFDVGLSILPMSHVYEFSI
                     GFLMFFLNGACIYYLEKPPAVTTLLPALKVVRLTIMLSVPIIMEKIYKNKIVPTFTSK
                     PLVAALYKIPFFRV"
BASE COUNT          316 a          226 c          234 g          254 t
ORIGIN      
        1 ctacgcggaa aaaaggaatc ttgtacaagg ccgccaccaa tggctttgag gtaaaggtag
       61 gtacaatctt gtttttatat attttttcca taatgatcgg aacactcagc ataatcgtga
      121 gcctgactac ctttagcgct ggaagcaagg tggtaacagc cggcggtttt tccagataat
      181 aaatgcacgc accattcaga aagaacatta aaaagcctat gctgaactca tagacatgcg
      241 acatgggtag aatagaaagg cctacatcga atttattgat gcgctgaaaa aactgaccgc
      301 cgattgccgt aaataccaaa ttccgatgag acagttccac tcccttggaa cggccggttg
      361 ttccagaggt ataaataatg gaagcggtgt cttcttcgtg tatctgtgct ccgggaaagg
      421 gtattacttc cccaagtgtt ccactcaata gcgacagatc atcaagtcga attacccgat
      481 ctagaccaag ggcagaagga ttggaaacct tctccgaaag ccgtgactgg acaatcattg
      541 agcttgctcc actgtggcgt atacaggttt ccgcctcttt tacactaaaa tcgggaagca
      601 agggaacagc gatagcgcca atggtaacaa tggcaaagta tgctatgccc caattgggag
      661 agctggggcc aaaaagaata actcgatctc ccttacgaat gcccattgtc cagagcatat
      721 ttcgtagcgt ttcaatgcgc gtgccaactt ccgcgtagtt aatgggagtg ccagaaacat
      781 acgaaagagc aggtctatcc gaaaactttt ttagactatt tgccaacatg gccgtaaacg
      841 tatacgaacc aaggtcatgt attgtttcca ttgtgtactc ctttgtaaag catggtagaa
      901 acgaccaggc gtctctcgcc ttaagtgtat gacatttttt tatgaactgt cattaaaata
      961 cggcggcaaa atgtcaaaaa tagtgaaaaa aaagggggac gatagccaaa aaaggcaatc
     1021 gtcacccctg
//
LOCUS       DAOQXQ010000681         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_202630_length_1025_cov_2.484536, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000681 DAOQXQ010000000
VERSION     DAOQXQ010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202630_length_1025_cov_2.484536"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="PKH81_09595"
     CDS             complement(<1..>1025)
                     /locus_tag="PKH81_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein"
                     /protein_id="HNQ98330.1"
                     /translation="VMVFALLVSCVSLYGLSVTARETIFIRQEVVALITGESMSVEKK
                     KRSVALRKRGMGLRFKLAFFTTTLVISVVLIVSIPIGIRFSANQERTLAKGLESRVQV
                     LLESLSSGARAYLPSQNILELGFLPGQMSALEEARYATITGGAIQQDSTGIDNVWATN
                     DPDIESKIDSGTYTPGRSRITGGENDDITAMVAVLDAEAERTVGELSRGISDLTQEGI
                     KLALKTDSESVHRRDEIQVISRQLEEKLNRELTRLSASGTGSYPRYDPDALSRDTTSY
                     VFYKPVLYRSGSDSRYVRGTVRVEISTLSLLEAVNKDRQELVQTTFYIALFAVLILAV
                     AALFFLH"
BASE COUNT          288 a          245 c          231 g          261 t
ORIGIN      
        1 aaatgcaaga agaagagggc tgccactgcc aggattaaaa ccgcaaacag tgcaatgtaa
       61 aaggttgtct gaacgagctc ctgccggtct ttattcaccg cttccagtaa actcaatgta
      121 gagatttcaa cacgaaccgt tccacgaaca taccgggagt ctgatccact tcgatatagt
      181 acaggcttat aaaagacata acttgtagta tcgcgggaaa gtgcatcagg atcatagcgt
      241 ggataggaac cggtgccgga tgctgacaga cgagttaatt cacgattcag tttttcttca
      301 agttgtcgtg atattacctg aatctcatcg cggcgatgaa cagattcact atctgttttt
      361 agagcaagtt taataccttc ctgggttaga tcgctaattc cccgagaaag ctcacccact
      421 gtccgttctg cctcggcgtc caacaccgcg accatcgcag tgatatcgtc attttctcca
      481 ccggttatac gggatcgacc tggcgtatag gtaccactat caatctttga ctcaatatct
      541 ggatcgttgg tcgcccacac attatcgatg ccggtagaat cctgctgtat cgctcctcca
      601 gttattgtcg catagcgcgc ttcctcaagt gcagacattt gtccgggaag gaacccgagt
      661 tcaaggatat tttgacttgg caaatacgca cgggctccag atgaaagact ctccaataaa
      721 acctgaacac gggattcaag tccttttgca agggtacgtt cctggttggc agagaaacgt
      781 attccaatcg gaatggacac aataagtacg acggagataa ccaaagttgt ggtaaaaaat
      841 gcaagtttga agcgaaggcc catcccccgt tttcgtaatg ctacggagcg ttttttcttt
      901 tcgaccgaca tggattctcc tgtaatcaag gctacaactt cttggcgaat aaatatagtt
      961 tcccgtgcgg taacagaaag accgtagagg gacacgcatg atactaacag agcaaagacc
     1021 attac
//
LOCUS       DAOQXQ010000682         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_202933_length_1024_cov_3.022704, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000682 DAOQXQ010000000
VERSION     DAOQXQ010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202933_length_1024_cov_3.022704"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKH81_09600"
     CDS             <1..262
                     /locus_tag="PKH81_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ98331.1"
                     /translation="HSVCFGLFHPAAVVFISERVPKRLLAVGLTIYTSVAVSMSSVIG
                     NVAGGFIIDRWGYRVLFVFFSVFPLLGVLFFMLMRTRKQGTH"
     gene            297..>1024
                     /locus_tag="PKH81_09605"
     CDS             297..>1024
                     /locus_tag="PKH81_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNQ98332.1"
                     /translation="MLGTIVNCIAVIIGSCVGILFARKVSEELSKVICSAAGIVTLII
                     GFQLAFKAQNIIYLALSLIIGGILGSWWDIDGKILSFGRFLERRFGGKGQAAEGQVSS
                     RFAYAFLNASVLFCVGAMAIVGSFKAGTEGDYTLLYTKSVLDGFMSVVFAAAMGVGTI
                     FSALSLLVYQGALTLASGVVKPFVSEQMILELTGIGGAFIVMIGINLLDLKKLKTANY
                     LPAMLVTILFVLADPFIAKIAGFFV"
BASE COUNT          199 a          205 c          258 g          362 t
ORIGIN      
        1 tcattcagta tgttttggtc tttttcatcc tgctgcggtt gtttttatca gcgaacgggt
       61 gcctaaacga ttattggccg ttggattaac aatctacact tcggtcgcgg taagtatgtc
      121 gtcggtaatc ggtaatgttg ccggtggttt tatcatagac aggtggggct atcgcgtatt
      181 gtttgtcttt ttttcagtct tcccgctgtt aggtgttttg ttttttatgc tcatgcgtac
      241 gcgcaaacag ggtacgcatt gacagccccc aagtacgtag ttagtatcag accattatgt
      301 taggaactat cgttaattgc attgcggtga taattggttc gtgtgtcggt atattattcg
      361 ctcgcaaggt atccgaggag ttatccaagg tcatctgctc tgcagcaggt attgtaacct
      421 tgataattgg ttttcagctt gcctttaagg ctcaaaatat tatctatctt gccctgtctc
      481 tgatcattgg ggggatattg ggctcctggt gggacattga tggcaagatt ctttcttttg
      541 gccgtttttt ggagcgtcgc tttggtggaa aaggacaagc ggcagagggt caggtatcct
      601 ctcgattcgc ctatgccttt ttaaacgcat cagttctttt ttgtgttggg gccatggcta
      661 tcgttggctc ttttaaggcg ggtaccgaag gcgattacac cctgttatat accaagtctg
      721 tactggacgg ctttatgtcg gtagtctttg ccgccgctat gggtgttggt acgatttttt
      781 ctgctctttc gctgctggtg tatcaggggg ccttgaccct tgcctccgga gtggtaaaac
      841 cctttgtttc cgaacagatg attcttgaat taacgggaat tggcggggct tttatcgtta
      901 tgatcgggat taacctgctt gatcttaaaa agctgaaaac tgccaattat ttaccagcca
      961 tgctggtaac tattctattt gttcttgcag atccttttat cgccaagatc gcgggctttt
     1021 ttgt
//
LOCUS       DAOQXQ010000683         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_203770_length_1022_cov_2.041365, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000683 DAOQXQ010000000
VERSION     DAOQXQ010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203770_length_1022_cov_2.041365"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1022
                     /gene="priA"
                     /locus_tag="PKH81_09610"
     CDS             77..>1022
                     /gene="priA"
                     /locus_tag="PKH81_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNQ98333.1"
                     /translation="MIPSGRRETGGVPVGIDDSDFSPVRHALSEEQIAAIDTITGESR
                     PKSVYLWGVTGSGKTEVFLQAAEHILNQGKDILYLVPEIALTHQVVEAVVNRFGTQAA
                     VLHSGLSGSKKLGEWMRILRGDARIVIGARSAVFAPVKNLGLIIIDEEHDGSYKSGTT
                     PRYHARTVAMRRATVTGATLVMGSATPSLEAWYQIRSGALVSCRLTKRLAGGAEPRLS
                     VVDLSNSNGPLSPELIEAIRTAKQSGRQTILFLNRRGFTHFYRCKTCGYELMCKNCSV
                     PLTLHRSISVMKCHYCGWQVPPPGSCPSCGSLDTAYAGF"
BASE COUNT          253 a          259 c          268 g          242 t
ORIGIN      
        1 tgggcctgat catattgacc tggcccgttg gatttcctct ttttaccttt gttcagtagg
       61 ggaagcgctt tcggcaatga ttccttcagg acgcagagaa acaggaggcg tgcctgtcgg
      121 cattgacgac agtgatttct cccctgtccg ccatgcgctt tccgaagagc aaatcgccgc
      181 tatagacacc attaccggag aatcgcggcc aaagagcgtg tacctttggg gggtaaccgg
      241 ttcagggaaa acagaagtct ttttacaggc agcagagcac atcctgaatc aaggaaagga
      301 catactgtat ctggtgccgg aaatcgcttt gacacatcaa gtagttgagg cggtagtcaa
      361 tcgctttggc acccaggccg cagtcctcca ctctggctta tcaggctcaa aaaaactggg
      421 ggaatggatg cgcattctcc gcggagatgc cagaattgtt atcggggccc gaagcgccgt
      481 ctttgcaccg gttaaaaacc ttggactaat cataatagac gaagagcacg atggttcata
      541 taagtctgga acaacgccgc gataccatgc acgaacggtt gcaatgagac gagctaccgt
      601 aaccggcgcg acattggtaa tgggaagcgc aacaccctct ctggaagcct ggtatcaaat
      661 tcgcagcggt gcccttgtct cctgccgact gacgaaacgc ctcgcaggag gagcggagcc
      721 tcgtttgtcc gtagtggatc tatcgaactc gaacggaccg ctgtcgccag agcttattga
      781 ggcaatcaga acagccaaac aaagcggaag acagactatt ctctttttaa atagacgtgg
      841 cttcactcat ttttatcgat gtaaaacctg tggctatgag ctgatgtgca agaactgctc
      901 ggtaccgctc accctgcatc gaagtatatc ggttatgaaa tgtcattatt gtggatggca
      961 agttcctccc ccgggaagct gtccttcctg tggatccctt gataccgcat atgccggctt
     1021 tg
//
LOCUS       DAOQXQ010000684         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_204000_length_1021_cov_2.745342, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000684 DAOQXQ010000000
VERSION     DAOQXQ010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204000_length_1021_cov_2.745342"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKH81_09615"
     CDS             <1..989
                     /locus_tag="PKH81_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HNQ98334.1"
                     /translation="RDMTPDYIGFIRRALSDIGWGHIPVISLSAQSLEKNPGFGISPA
                     LIHRAMMSIMLGDLLMRVLYRTRPYEAVSGSANELYEKFNTRCVEQIRSLSIRGYNRL
                     VRDIVREFDELPLRKVHKARVGVVGEILVKFHPTANNDIFGLIEREGAECVVPDLADF
                     LFYSFVNGVYKYQKLAFPKKAERVARLLVWFLERYRTEIKRSLTRSKRFTPPESIYKL
                     QEGVDDIVQLGNMTGEGWFLTAEMIELIHTGVPSIACVQPFACLPNHVTGKGMIKELR
                     RRFPESNIAAIDYDPGASEVNQLNRLKLLLANAPIGSHPDETKIGPARLVKK"
BASE COUNT          238 a          249 c          270 g          264 t
ORIGIN      
        1 accgggatat gaccccagac tacatcggat ttatccggcg tgccctttcc gatataggat
       61 ggggtcatat cccggtaata tctctgtctg ctcaatcgct cgaaaagaat ccgggttttg
      121 gtattagccc agcccttatt catcgcgcaa tgatgtccat catgcttggg gatttattga
      181 tgcgcgtgct ctaccgaacc cgtccctacg aggccgtcag cggttcggct aacgagctgt
      241 atgaaaagtt taatacccgc tgtgtggagc agattcgatc cttgtcaatt cggggatata
      301 accggctggt acgcgacatt gtgcgagaat tcgacgagct accgctacgc aaggtacaca
      361 aggcccgcgt tggcgttgta ggagagattc tggttaagtt ccatccaacc gcgaacaacg
      421 atatctttgg cctgatagag cgggagggcg ctgaatgtgt ggtccccgat cttgcggact
      481 ttttgttcta ttcctttgtt aatggggtct acaagtatca aaaacttgcc ttccccaaaa
      541 aggccgagcg agttgcccgt ctgttggtgt ggttccttga acgctaccga accgagatta
      601 aacgctctct tacgcgcagc aagcggttta ctccgccaga gtccatctat aaactccagg
      661 aaggggtaga tgatatcgtt cagttaggca atatgaccgg ggagggttgg ttcctgactg
      721 ccgagatgat cgagctcatt cataccggtg tgcccagtat agcctgtgtg cagccgtttg
      781 cgtgtttacc caaccatgta accggtaaag gcatgattaa ggaactgaga cgacgtttcc
      841 ctgaatcaaa tattgccgcg atcgactacg accctggagc aagcgaggtt aatcagctta
      901 accgtcttaa acttctgctt gccaatgcgc cgattggctc acatccggac gagaccaaaa
      961 taggtcctgc ccgtttggtc aagaagtagg gattttttgc cgatattggg ggagatatga
     1021 g
//
LOCUS       DAOQXQ010000685         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_205764_length_1016_cov_3.559834, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000685 DAOQXQ010000000
VERSION     DAOQXQ010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205764_length_1016_cov_3.559834"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKH81_09620"
     CDS             complement(<1..943)
                     /locus_tag="PKH81_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNQ98335.1"
                     /translation="MKHYRITTPKKVLILGSGAPRIGQAGEYDNAGLQALRALKDGGI
                     TTVLINPNMTAVQTSASAADSIYFLPVTAEFAAKVIEKEQPDSLIVSYGGKDALYCAL
                     ELEKEGILEKHSIQILGTSLSNLALAANRDEFSRAMKAAGIPTPASFPAATEAEAIEL
                     SNGLSFPVLMRTNGSDEAELCNTPQELSAYFNAHGPLILEEWLGGWKEIECVTVRDAM
                     DNCVLSCTLECIDPVGIHSGDSIVVSPCQSLTDCELQAIRDTAFATARALGVIGEVSL
                     RFAKDPASAAFRLLEANPRVSRSTALASKATGVPLGYL"
BASE COUNT          240 a          238 c          284 g          254 t
ORIGIN      
        1 agaggtagcc gaggggaact cctgttgctt tggatgcaag ggccgtgctg cgggatactc
       61 gtggatttgc ttccagtaga cgaaaggccg cagaagcggg atctttggca aaacgaaggc
      121 tcacttctcc aattacccca agagcgcggg ctgtagcaaa ggcagtatcg cggatggctt
      181 gcagctcgca gtcggtaagg ctttgacagg gagaaaccac aatagaatca cctgaatgga
      241 tgcctaccgg atcgatacat tccagcgtgc aggaaaggac acagttgtcc attgcgtccc
      301 ggacggttac gcattcgatc tccttccatc ctccgagcca ttcttccagg atcaagggcc
      361 cgtgtgcgtt aaaataggcg gagagttctt gcggggtgtt gcatagttcc gcttcatcgc
      421 taccgttagt gcgcataagg acgggaaatg aaagaccatt agaaagctcg attgcttctg
      481 cctcggttgc agcggggaac gatgccgggg taggaattcc ggcagccttc atcgcacgag
      541 aaaactcgtc gcgattggcc gccagggcga gattactaag gctggtgccc agaatttgaa
      601 ttgaatgttt ttccagaatc ccttcttttt ccaattccag ggcgcaatac aaagcgtcct
      661 ttcctccata ggaaacaata agagagtcag gttgttcctt ttcgattacc tttgcggcaa
      721 actctgcggt aactggtaaa aaatagattg aatcggcagc gctagcagat gtttgaactg
      781 cagtcatgtt gggattgata agtacggtgg taatgccgcc atcttttaag gctcggaggg
      841 cttgaaggcc cgcgttgtca tattctcctg cttgtccgat tcgtggagct ccggagccaa
      901 ggataagaac tttttttggt gtggtgatgc gatagtgctt catgtctggg gtcctccatt
      961 gtctacaagc tgtacaaact ctgtccaaag aaaatccgct tcatgtggac cggggt
//
LOCUS       DAOQXQ010000686         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_205765_length_1016_cov_3.499480, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000686 DAOQXQ010000000
VERSION     DAOQXQ010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205765_length_1016_cov_3.499480"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          192 c          216 g          312 t
ORIGIN      
        1 tgccgcatcc attgagggca ggcgttgctt cctttcgatc cgtaatactc ggttcggatt
       61 taagatcgcg acccaccatt tgtagatgaa cagaaactca gctttggtgg tctttgatca
      121 atttcaacct tattgatgac aaatgcaagt cgttaacccc cagatgttga gagattattg
      181 aatggcgatc gaaagcgcgc acaaatctga aggccaattt ctccccttaa aattaggacc
      241 gctgcttcct gaaatcgagt atatcaattt tctgccatat atgatagatt acaaaccaat
      301 ttcattgttc ctgcttatgc tgttttaaca agataaaact tgcttgatta tttgtttcaa
      361 gacagattta caagttgtaa tggtttggaa ggcgatctta ccttataatt tcagttcatt
      421 ttcttatcaa ttaataaccg acgaagctgc cttatgaaat actggataaa atcacggagt
      481 atatcaaaaa cggaagcaat acagtatata actattgccc ttctgatata ctccgctatt
      541 tatttggttt tctgcgagat ccacatgccc tccgtggatg acaacaggtg gagggatatt
      601 caactgtcgg aggcggaact gcgggatacg tctttgataa agaaaatcac ggtggagaat
      661 attgaacagg acaatcttag ttattgtaat gatcagagca taatgggttt tttcaagaaa
      721 ttattgttcg ggcagaaaca agtccctttc ggttgtgcaa accggtcatg gctagatttt
      781 aagtcccggt ttaatcctgg agatgagctt tggacgttca gatattcctg gaatagtggg
      841 ccgcttgcat cgctatatat tgaagggttt tgcatcgttc ggaagagcaa aatcattcgt
      901 acatgggcaa cggagagtgt tacctcgtga agtcagcaag ccacttctat tggtatgaat
      961 aatcattctc aaagggaagt aaatacagaa gttttttaac ctccatcaaa ggtatt
//
LOCUS       DAOQXQ010000687         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_206283_length_1015_cov_2.047917, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000687 DAOQXQ010000000
VERSION     DAOQXQ010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206283_length_1015_cov_2.047917"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKH81_09625"
     CDS             <1..597
                     /locus_tag="PKH81_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98336.1"
                     /translation="ESKPNCWAWEAGTSWVLPLTNPRWRFTVNLEYSHADKWTYNRWQ
                     PYLTMYQRQVLTGGWDGLDIPLGHPEGGDVDQIGLRFVAMTRNGKKIEASYTWISKGP
                     VYLGRIEEIQVQGETRYIPVYYDYDSWTSEGELDRLLGNTRKHSHVIALSLVWPVGKN
                     WSLKGDVDYRLILNAAHVKGRVDHELVWKAGAARYFGK"
     gene            complement(598..>1015)
                     /locus_tag="PKH81_09630"
     CDS             complement(598..>1015)
                     /locus_tag="PKH81_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNQ98337.1"
                     /translation="EESPFTLFDSLVQGDLEQAIEIMNKLTLSRESSPVQTIAGLTWC
                     FRRLADWHALSDSGMTDDFSLKKAGFSSKKAITQYRQAAKRWDSMDSQRLLALLADTD
                     LEIRRTGTAVQDTLIQGALYSICCKRGVPLEGVEYR"
BASE COUNT          276 a          252 c          257 g          230 t
ORIGIN      
        1 gagtcaaaac caaactgctg ggcctgggaa gcagggacaa gctgggttct tccattaact
       61 aatccccgtt ggcgattcac ggtcaacctg gagtatagcc acgcagataa gtggacctat
      121 aatcgctggc aaccctatct aacaatgtat caacgacagg ttcttaccgg gggctgggat
      181 ggacttgata taccgctggg tcatccagaa ggcggagacg tagaccaaat aggacttcgc
      241 tttgtcgcca tgacgcgcaa cggtaaaaaa attgaagctt cttatacctg gatatccaag
      301 ggcccggtgt atctgggaag aattgaagag atacaggtac agggcgaaac cagatatatt
      361 cccgtgtatt acgattatga ttcctggaca agcgagggcg aacttgatcg cctactcgga
      421 aacacccgaa aacactctca tgtaattgcc ctctccctgg tatggccggt tggaaaaaac
      481 tggtcgctca aaggagatgt cgattaccgg ctcattctga acgcagccca tgttaaagga
      541 agagttgatc atgaactggt atggaaagcg ggcgctgccc gctattttgg caagtaacta
      601 acgatactca acaccttcca gggggacccc tcgtttacaa cagatcgagt acagggctcc
      661 ctgaataagc gtatcttgca cagccgttcc cgtccgacga atctcaagat ccgtatctgc
      721 aagaagggcg aggaggcgct gactgtccat actgtcccat cgtttagcag cttgccgata
      781 ctgcgtaatt gcctttttac tggaaaatcc cgcttttttt agcgaaaaat catcggtcat
      841 gccgctgtcc gacaaggcgt gccaatctgc aagccgcctg aaacaccagg tgagaccggc
      901 aatcgtctgg acgggagagg attcccgcga gagagtcaat ttattcataa tctcgattgc
      961 ctgctccaag tcgccctgga caagagaatc aaaaagagta aagggacttt cttcg
//
LOCUS       DAOQXQ010000688         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_208117_length_1010_cov_2.326702, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000688 DAOQXQ010000000
VERSION     DAOQXQ010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208117_length_1010_cov_2.326702"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PKH81_09635"
     CDS             complement(<1..167)
                     /locus_tag="PKH81_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNQ98338.1"
                     /translation="MEVASMAKIYLDPITRIEGHLSIEVDVQNGKVVDAKSKGDMFRG
                     FEKILQGRNPV"
     gene            complement(145..>1010)
                     /locus_tag="PKH81_09640"
     CDS             complement(145..>1010)
                     /locus_tag="PKH81_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HNQ98339.1"
                     /translation="SCSGCSVSILNAIDPGIATVITEHISLNYHQTLMGGTGDAAIRV
                     LEEAVKKQRKDFVLVVEGSIPVDSEEYCTIGMLNGKHVSALEWVRELGKNAKAILAFG
                     TCAAYGGIPAAESRKTGKNPTGAKSLAEILPNAAIINVPGCPPHPDWMIGTVLHYLLK
                     GMPELDEYKRPKLFFGKTVHDQCEHLPDYKRGRFAKHWGDEGCLYLLGCLGMDSGCDI
                     PKRKWLGGVNSCTGCGSGCIGCTEPVFPDYGQRGLYKHLTASLDEINSLEEPQIRAAV
                     LNLRNGGSING"
BASE COUNT          193 a          290 c          290 g          237 t
ORIGIN      
        1 tcgacgggat tacgcccctg aagtatcttt tcgaagccgc ggaacatatc gcccttgctc
       61 ttggcgtcca cgaccttccc gttctggaca tcaacctcta tcgaaagatg cccttcaatt
      121 ctcgtgatgg gatcaagata aatcttagcc attgatgcta cctccattcc tgagattcaa
      181 caccgccgcg cggatctggg gctcctcgag agagttgatt tcgtcgaggc ttgcggtgag
      241 atgcttgtag agaccacgct ggccataatc ggggaacacg ggctcggtgc atccgataca
      301 tccggaaccg cagcccgtgc aggagttaac gccgcccagc cactttctct tcgggatgtc
      361 gcatccactg tccatgccga gacagccgag caggtacagg cagccctcgt caccccagtg
      421 cttggcaaag cggccccgtt tgtaatcggg aaggtgctcg cactggtcgt ggacggtttt
      481 cccgaaaaaa agtttcggtc gtttgtattc gtcgagctcg ggcatgccct tgagcaggta
      541 atgcaggacc gtgccgatca tccagtccgg atggggcggg caaccgggca cgttgattat
      601 cgcggcgttc ggaagaattt cggccaggga tttggccccc gtggggttct tgccggtctt
      661 cctgctttcc gccgcgggta tgccgccata ggccgcgcat gtgccgaagg ccaggatcgc
      721 cttggcgttt ttcccgagtt cgcggaccca ttcgagggca ctgacatgct ttccgttcaa
      781 catgccgatg gtgcagtatt cctcggagtc aaccggtatt gatccctcga ccacaaggac
      841 gaaatccttg cgttgttttt ttacggcttc ctcgaggacc cggattgccg cgtccccagt
      901 gccgcccatc agcgtttgat gatagttgag gctgatgtgc tcggtgatga cggtcgcgat
      961 accgggatca atcgcgttga gtatagaaac tgaacagccg gagcaggact
//
LOCUS       DAOQXQ010000689         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_208755_length_1008_cov_2.754460, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000689 DAOQXQ010000000
VERSION     DAOQXQ010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208755_length_1008_cov_2.754460"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1008
                     /locus_tag="PKH81_09645"
     CDS             <1..>1008
                     /locus_tag="PKH81_09645"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNQ98340.1"
                     /translation="VRVSDQFDPVSFAERLSSAGYLRVPRVTVKGEFALRGEVLDIFL
                     PGDVDAHRIVFDFDRISAIKAFNPEDQSSTGTVSSLTIYPARELLWTEERIATLEATL
                     TALPEFSERSACIIEHLRERGGFEGEELFYPLAWDGKHSILDYLGSDRTVIYVDFDRQ
                     ENARESLEREYLGLYRKHRGAGGESERFPLLAEVPDPSHELLDFRSVTESFERSIYLR
                     TIHRDQHANSDRVRLSCDPPRSFFGNMVFFREELATLIKDGWRVVVFAESEHQAARVA
                     EIIKDETIDILPLAISTGFGVPDLKLLIIQEGEIFGRRRHIPRSVKHAKSAVIDTFVE
                     LN"
BASE COUNT          223 a          286 c          252 g          247 t
ORIGIN      
        1 gtgcgggtga gcgaccaatt tgatcctgtt tcctttgccg aacgcctttc ctcagcgggc
       61 taccttcggg ttccccgcgt aaccgtaaag ggcgagtttg cccttcgcgg agaggtgttg
      121 gatatctttc ttcccggaga tgtagacgca caccgcatcg tgttcgactt tgaccgaatc
      181 tctgcgatca aggcctttaa tcccgaagac caaagttcta cgggaacggt ttcttcgcta
      241 actatctatc ccgcccgcga gctgttatgg acagaagagc gtatagccac acttgaagca
      301 acacttaccg cgcttcccga gttttccgaa cgctctgctt gcataattga acacctacgg
      361 gagcggggcg gctttgaagg ggaagagctt ttttatcccc tggcctggga tggaaagcat
      421 tccatactgg actatctggg aagtgaccgc accgttatct atgtagactt tgaccgtcaa
      481 gagaacgcac gggaaagcct tgaacgcgag taccttggcc tgtatcgcaa gcatcgtggg
      541 gccggcgggg aaagcgagcg cttcccgctc ctggcggaag tacccgatcc ctcgcatgaa
      601 cttcttgact ttcgctcagt tactgaatcc tttgaacggt ccatttacct tagaaccatt
      661 caccgcgacc aacatgccaa ctctgatcgc gtgcgccttt cctgcgatcc ccccaggagt
      721 ttttttggca acatggtctt ctttcgcgaa gaacttgcta cccttattaa agacggctgg
      781 cgcgtcgtcg tctttgcgga aagcgaacat caagcggccc gcgttgccga aataataaaa
      841 gacgaaacga tcgacattct tcccctggcc atatccaccg ggtttggcgt tcccgaccta
      901 aagctattga taattcagga aggcgaaatc ttcggccgcc gccgccacat tcctcgctcg
      961 gtaaagcatg ccaagagcgc ggttatcgac acctttgttg aactaaat
//
LOCUS       DAOQXQ010000690         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_1032
            NODE_209156_length_1007_cov_2.370798, whole genome shotgun
            sequence.
ACCESSION   DAOQXQ010000690 DAOQXQ010000000
VERSION     DAOQXQ010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852375
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:54:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,931
            CDSs (total)                      :: 1,902
            Genes (coding)                    :: 1,885
            CDSs (with protein)               :: 1,885
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 7 of 17
            Pseudo Genes (incomplete)         :: 8 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209156_length_1007_cov_2.370798"
                     /isolate="AMR_MDS_1032"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..248)
                     /locus_tag="PKH81_09650"
     CDS             complement(18..248)
                     /locus_tag="PKH81_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98341.1"
                     /translation="MIYSFFMEEIITAPKDSKRRMLIGELTVFLLVAIAITLNIPKNP
                     VMNLIFLGLGIILEAGSIYTQMVRHFDIKYRI"
     gene            330..>1007
                     /locus_tag="PKH81_09655"
     CDS             330..>1007
                     /locus_tag="PKH81_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNQ98342.1"
                     /translation="MLDVLSILSGSDLFSGLSARSLERIANHLKPVRFDQDVVICREG
                     DIGRTMYIIVSGSVSVSSDMGWGQRELDRKGPGSVFGEMALISNDVRSATVRTLESTE
                     CLQLDNESFDALLDKDLDLAQRIAKIMTRRFSTLVHKTSSELLGAYRALTFALANITE
                     SRDPETGAHLERTRNYCVLLAEALYREERYKGLITPQFIDGLYQATPLHDVGKVAIPD
                     AILFKPEG"
BASE COUNT          240 a          216 c          256 g          295 t
ORIGIN      
        1 aacttgtttg tcaggcgcta gatacgatat ttgatatcga aatgacgaac catctgtgtg
       61 tagattgaac cggcttccag gattatccca aggcccagaa atatcaggtt cataaccggg
      121 tttttaggaa tattcagggt aatggcaatg gcgacaagga gaaaaacagt tagttctcca
      181 atgagcatac ggcgtttgct gtctttgggg gcggtgatta tttcttccat aaaaaaagag
      241 tatatcatac tattggtcat acttaccctt ttttacgctt gttttagggc ggaaacgggt
      301 ggatactttt tatcagcagg aggaatctca tgcttgatgt cctgtccatt ctttccggaa
      361 gtgatttgtt ttcggggcta tccgctcgtt ctcttgaacg cattgccaac catcttaaac
      421 cggtccgctt tgatcaggat gtggttattt gtcgggaagg cgatattggt cgaaccatgt
      481 atattattgt ttccggcagc gtttctgttt caagcgatat gggatggggc cagcgggagc
      541 tcgatcgtaa aggtcctggc tcagtctttg gagaaatggc tcttatttcc aacgatgttc
      601 gctccgcgac cgtgcgaacc ctggagtcaa ctgaatgtct gcagcttgat aacgaaagct
      661 ttgatgcctt gctcgataag gatcttgatc ttgcccagag gattgccaag attatgacca
      721 gaaggttttc caccctggta cacaaaacct ccagcgagct tctgggtgcg tatcgggcgc
      781 tgacctttgc gcttgccaat ataaccgaat ccagagatcc ggaaacaggg gctcatcttg
      841 agcggaccag aaattattgt gtgcttctgg cagaagccct ctatcgtgaa gagcgttata
      901 agggtttgat tacccctcaa tttatcgatg gtttgtatca ggcgaccccg cttcatgatg
      961 taggcaaggt tgctattccg gacgcgattc tctttaagcc ggaagga
//
